LOCUS       DAUVUY010000001        87423 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.001, whole genome shotgun sequence.
ACCESSION   DAUVUY010000001 DAUVUY010000000
VERSION     DAUVUY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 87423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87423
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.001"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..504
                     /locus_tag="VOX58_000001"
     CDS             <1..504
                     /locus_tag="VOX58_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HES5877283.1"
                     /translation="LSPQLVTYLAALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQI
                     DNTLVESIYKALTYKGIFSEVEKEHLSTGAYQTKNALYSNDELETLLNYNKYLYLKAA
                     KHIKKGHFLINPYTSDGKTVQGDQLKAITRFEADLDMAQARRLVTLPAKEKKECFLTL
                     MRKESHL"
     gene            465..4133
                     /gene="addA"
                     /locus_tag="VOX58_000002"
     CDS             465..4133
                     /gene="addA"
                     /locus_tag="VOX58_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HES5877284.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERCSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLGQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDITHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYSIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNIFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLPLALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRL
                     QTPQLLNPDDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKVAYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKVKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALREIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
     gene            4273..5085
                     /locus_tag="VOX58_000003"
     CDS             4273..5085
                     /locus_tag="VOX58_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HES5877285.1"
                     /translation="MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAV
                     SPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKAD
                     LAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
                     EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSL
                     KTSDGDAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
     gene            5226..5402
                     /locus_tag="VOX58_000004"
     CDS             5226..5402
                     /locus_tag="VOX58_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HES5877286.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(5530..5892)
                     /gene="mscL"
                     /locus_tag="VOX58_000005"
     CDS             complement(5530..5892)
                     /gene="mscL"
                     /locus_tag="VOX58_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HES5877287.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAA
                     APTQEELLTEIRDLLAQK"
     gene            6022..7836
                     /gene="dnaG"
                     /locus_tag="VOX58_000006"
     CDS             6022..7836
                     /gene="dnaG"
                     /locus_tag="VOX58_000006"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HES5877288.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADMEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPIPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     EILYQRLKQQGYITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            7845..8954
                     /gene="rpoD"
                     /locus_tag="VOX58_000007"
     CDS             7845..8954
                     /gene="rpoD"
                     /locus_tag="VOX58_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HES5877289.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            9190..9528
                     /locus_tag="VOX58_000008"
     CDS             9190..9528
                     /locus_tag="VOX58_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HES5877290.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            9666..10520
                     /gene="rfbD"
                     /locus_tag="VOX58_000009"
     CDS             9666..10520
                     /gene="rfbD"
                     /locus_tag="VOX58_000009"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HES5877291.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFAMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            10639..11793
                     /locus_tag="VOX58_000010"
     CDS             10639..11793
                     /locus_tag="VOX58_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HES5877292.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEDNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDRLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            11783..12715
                     /locus_tag="VOX58_000011"
     CDS             11783..12715
                     /locus_tag="VOX58_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HES5877293.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            12718..13521
                     /locus_tag="VOX58_000012"
     CDS             12718..13521
                     /locus_tag="VOX58_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5877294.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYVVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            13521..14726
                     /locus_tag="VOX58_000013"
     CDS             13521..14726
                     /locus_tag="VOX58_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5877295.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            14751..15758
                     /locus_tag="VOX58_000014"
     CDS             14751..15758
                     /locus_tag="VOX58_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HES5877296.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTVYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEMSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            15755..17500
                     /locus_tag="VOX58_000015"
     CDS             15755..17500
                     /locus_tag="VOX58_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HES5877297.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMGPYFENFEEKEAVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSVFETDDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLFVYI
                     AWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLMEKLK"
     gene            17497..19971
                     /locus_tag="VOX58_000016"
     CDS             17497..19971
                     /locus_tag="VOX58_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HES5877298.1"
                     /translation="MIKDTFLKTNWLNISHHIILLVFGFYFSFYSLAKELVSSTAQPV
                     NYYAHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVRAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLKSNKPSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVALLAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHT
                     ISEFYQGKNRIAMHPASANNFNRKTVYSNLGFSKFLALSGSKDKFKNIENVGLLTSDK
                     TVYNNILSLINPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTSYARL
                     LSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFIWSNG
                     TNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKAIQND
                     LKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            20150..20845
                     /locus_tag="VOX58_000017"
     CDS             20150..20845
                     /locus_tag="VOX58_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HES5877299.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            20847..21188
                     /locus_tag="VOX58_000018"
     CDS             20847..21188
                     /locus_tag="VOX58_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HES5877300.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILALLI
                     LLFTWLPNILNKMSHFLGIHSPTNMLFFLGFCLSLAIIFSLTNNISLQNDKVKRLTQE
                     VALMKKEKTND"
     gene            21181..22467
                     /locus_tag="VOX58_000019"
     CDS             21181..22467
                     /locus_tag="VOX58_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HES5877301.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSADSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFMVYTAII
                     LYSKNLTLALVAVCIVSLVFIMYYDIGHSKKFQKLMFSELLSNISFQNSLKLLKESFP
                     LFLNGFLIIYIYTQPKYAIELMTTLGEVALGSQTIFNILFMPAFVMNLLILFFRPHIT
                     QMAIALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSILYGTNLTDYWVD
                     FMLIMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLFVMKYHILGAA
                     LSFLITMLVWLGLSIMIYLFIMNRFKKGRVNATIYD"
     gene            22448..23944
                     /locus_tag="VOX58_000020"
     CDS             22448..23944
                     /locus_tag="VOX58_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HES5877302.1"
                     /translation="MQQSMTKVKYLWKENNNYIYSTFLTLLLTVILYANSLNLPLKFI
                     SLLFVVSTLLIVFLPKKLEVATIIFILVFGTISAIISPINDIPDEYVHYSRTVYISEG
                     DINLTNNNKKLRISKDVDKLIKQSGKTFITSNLKATKHSTREYSYPYIKGTNAYYSFS
                     YIPQALGILVGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVIAVVTLLP
                     MNIYLAASFNQDGFAIGLVLVTIGLFINLLSSKDKSNYNTKFFLYLVLCGLLVLSKFT
                     YFLLVCLPLFIPNEKFGKNTKLVILKKLGGLLLIFLFAAMWFRLYGQVKTPYVADFLK
                     EVNVSQQVKNMLESPIVYSSIIIRHMVINLINMNNIFQFGALSYGITNLFPLYVCFFF
                     FVYISNASKITINIVEKMGIIFVISAIIGATVLAMYLTWTPVGSSTVLGVQSRYLIGI
                     IPLVLLLFSSQQQKFKQIEDILSDKLAIHVSLLFILAMLMSTIFRYYH"
     gene            24038..25261
                     /gene="pepT"
                     /locus_tag="VOX58_000021"
     CDS             24038..25261
                     /gene="pepT"
                     /locus_tag="VOX58_000021"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HES5877303.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALELAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            25302..25790
                     /locus_tag="VOX58_000022"
     CDS             25302..25790
                     /locus_tag="VOX58_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HES5877304.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHFEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(25777..25974)
                     /locus_tag="VOX58_000023"
     CDS             complement(25777..25974)
                     /locus_tag="VOX58_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HES5877305.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            26023..26499
                     /locus_tag="VOX58_000024"
     CDS             26023..26499
                     /locus_tag="VOX58_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HES5877306.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKASSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            26514..27194
                     /locus_tag="VOX58_000025"
     CDS             26514..27194
                     /locus_tag="VOX58_000025"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HES5877307.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPISFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWV
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    27218..27341
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            27356..27886
                     /gene="infC"
                     /locus_tag="VOX58_000026"
     CDS             27356..27886
                     /gene="infC"
                     /locus_tag="VOX58_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HES5877308.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            27928..28125
                     /gene="rpmI"
                     /locus_tag="VOX58_000027"
     CDS             27928..28125
                     /gene="rpmI"
                     /locus_tag="VOX58_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HES5877309.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            28184..28543
                     /gene="rplT"
                     /locus_tag="VOX58_000028"
     CDS             28184..28543
                     /gene="rplT"
                     /locus_tag="VOX58_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HES5877310.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(28834..31044)
                     /locus_tag="VOX58_000029"
     CDS             complement(28834..31044)
                     /locus_tag="VOX58_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HES5877311.1"
                     /translation="MTSLGKNDKEVATVKKFKTLITGFINTRLGFIITLLFCYWIKTL
                     WAYHTDFSLDLGNIYQVFLTIINPIPLAFLLLGVALYVKNTRAFYICSWVVYIILNIL
                     LISNSIYYREFSDFITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIT
                     KKIKNDQRPFNKRAAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPA
                     FTLYSGNQTYQAQKERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLES
                     FQQFLIDYKLKEGDKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSL
                     FGLNSGSFMVNYGGENTQFATPSILTQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYF
                     FDSSYFSKQNSKNSFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGE
                     SSEEGFPLAKTDDETINGYFATANYLDAALKSFFDYLKATGLYENSIFVLYGDHYGIS
                     NSRNSSLAPLLGKDSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLL
                     HILGIDTSQFVQLGQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNP
                     DEMTIAKTKEIRSAVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKD
                     ISAKLGSESTSLYSKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNPKNKE"
     gene            31152..32315
                     /locus_tag="VOX58_000030"
     CDS             31152..32315
                     /locus_tag="VOX58_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HES5877312.1"
                     /translation="MNKLYIDSFVEKKLTAGVQLLDEKDFSNIKEKNQLVQLVTKSNR
                     PIGTAYISKQNKGIGWYLGPEKIDLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQ
                     EGDGFGGVTIDLYKDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDT
                     ETAHLYGELAQETFSILENGIAYQVFLNEGLMTGVFLDQHDVRRALVDGLAMGKSLLN
                     LFSYTAAFSVAAAMGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYF
                     KYAKRKKLIFDVIVIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAA
                     NMTVSQFKKQIIKGFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            32312..32998
                     /locus_tag="VOX58_000031"
     CDS             32312..32998
                     /locus_tag="VOX58_000031"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HES5877313.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            33092..34258
                     /gene="aroC"
                     /locus_tag="VOX58_000032"
     CDS             33092..34258
                     /gene="aroC"
                     /locus_tag="VOX58_000032"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HES5877314.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            34319..34660
                     /locus_tag="VOX58_000033"
     CDS             34319..34660
                     /locus_tag="VOX58_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HES5877315.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            34882..36234
                     /gene="gorA"
                     /locus_tag="VOX58_000034"
     CDS             34882..36234
                     /gene="gorA"
                     /locus_tag="VOX58_000034"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HES5877316.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKDKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(36322..37590)
                     /locus_tag="VOX58_000035"
     CDS             complement(36322..37590)
                     /locus_tag="VOX58_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HES5877317.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(37620..38060)
                     /locus_tag="VOX58_000036"
     CDS             complement(37620..38060)
                     /locus_tag="VOX58_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877318.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSATEGTQLPNATGAEDANTGLPTNS"
     gene            38295..39437
                     /locus_tag="VOX58_000037"
     CDS             38295..39437
                     /locus_tag="VOX58_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HES5877319.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            39449..40663
                     /gene="thiI"
                     /locus_tag="VOX58_000038"
     CDS             39449..40663
                     /gene="thiI"
                     /locus_tag="VOX58_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HES5877320.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAIAVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            40701..41993
                     /locus_tag="VOX58_000039"
     CDS             40701..41993
                     /locus_tag="VOX58_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HES5877321.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNKLAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    42098..42172
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            42207..42521
                     /gene="rplU"
                     /locus_tag="VOX58_000040"
     CDS             42207..42521
                     /gene="rplU"
                     /locus_tag="VOX58_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HES5877322.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            42533..42859
                     /locus_tag="VOX58_000041"
     CDS             42533..42859
                     /locus_tag="VOX58_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HES5877323.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            42887..43180
                     /gene="rpmA"
                     /locus_tag="VOX58_000042"
     CDS             42887..43180
                     /gene="rpmA"
                     /locus_tag="VOX58_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HES5877324.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            43538..44452
                     /locus_tag="VOX58_000043"
     CDS             43538..44452
                     /locus_tag="VOX58_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HES5877325.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLSDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            44449..44907
                     /locus_tag="VOX58_000044"
     CDS             44449..44907
                     /locus_tag="VOX58_000044"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HES5877326.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMVHLDFVDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            44897..45787
                     /locus_tag="VOX58_000045"
     CDS             44897..45787
                     /locus_tag="VOX58_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HES5877327.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDTLAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            46182..46703
                     /gene="pyrR"
                     /locus_tag="VOX58_000046"
     CDS             46182..46703
                     /gene="pyrR"
                     /locus_tag="VOX58_000046"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HES5877328.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            46719..47978
                     /locus_tag="VOX58_000047"
     CDS             46719..47978
                     /locus_tag="VOX58_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HES5877329.1"
                     /translation="MKDVIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKVIGNDWIDKILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            48039..48974
                     /locus_tag="VOX58_000048"
     CDS             48039..48974
                     /locus_tag="VOX58_000048"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HES5877330.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            49018..50100
                     /gene="carA"
                     /locus_tag="VOX58_000049"
     CDS             49018..50100
                     /gene="carA"
                     /locus_tag="VOX58_000049"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HES5877331.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            50346..53522
                     /gene="carB"
                     /locus_tag="VOX58_000050"
     CDS             50346..53522
                     /gene="carB"
                     /locus_tag="VOX58_000050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HES5877332.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPTLDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQDLQMHVEHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNIEAI"
     gene            53729..55000
                     /locus_tag="VOX58_000051"
     CDS             53729..55000
                     /locus_tag="VOX58_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HES5877333.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVAVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            55000..55710
                     /locus_tag="VOX58_000052"
     CDS             55000..55710
                     /locus_tag="VOX58_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5877334.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            55722..56942
                     /locus_tag="VOX58_000053"
     CDS             55722..56942
                     /locus_tag="VOX58_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5877335.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGEGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            57196..58929
                     /locus_tag="VOX58_000054"
     CDS             57196..58929
                     /locus_tag="VOX58_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HES5877336.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTLVVVSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     SRLVRIISSLY"
     gene            59056..59328
                     /gene="rpsP"
                     /locus_tag="VOX58_000055"
     CDS             59056..59328
                     /gene="rpsP"
                     /locus_tag="VOX58_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HES5877337.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            59338..59577
                     /locus_tag="VOX58_000056"
     CDS             59338..59577
                     /locus_tag="VOX58_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HES5877338.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
     gene            60509..63535
                     /locus_tag="VOX58_000057"
     CDS             60509..63535
                     /locus_tag="VOX58_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HES5877339.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQITSGSQLEQSKESLSLNKTVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTIGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINRAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            63555..63956
                     /locus_tag="VOX58_000058"
     CDS             63555..63956
                     /locus_tag="VOX58_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HES5877340.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(64146..65021)
                     /locus_tag="VOX58_000059"
     CDS             complement(64146..65021)
                     /locus_tag="VOX58_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HES5877341.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCALHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLKESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSGIEVN"
     gene            65157..65678
                     /locus_tag="VOX58_000060"
     CDS             65157..65678
                     /locus_tag="VOX58_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HES5877342.1"
                     /translation="MTKDRQIKKTKTAIYSAFLALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMS
                     ANELLKYYLELIK"
     gene            65893..66411
                     /gene="rimM"
                     /locus_tag="VOX58_000061"
     CDS             65893..66411
                     /gene="rimM"
                     /locus_tag="VOX58_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HES5877343.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DQFVQEVTIVSHRKQKHFDIIKLKDMYHINAIEKYKGYTLKVSKANQGDLQEGEFYYH
                     QIIGMAVYEKDRLIGYVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            66401..67132
                     /gene="trmD"
                     /locus_tag="VOX58_000062"
     CDS             66401..67132
                     /gene="trmD"
                     /locus_tag="VOX58_000062"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HES5877344.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALGQGED"
     gene            67132..68124
                     /locus_tag="VOX58_000063"
     CDS             67132..68124
                     /locus_tag="VOX58_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HES5877345.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGVELTENLIKQLNRFEDRITICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLTTSTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            68303..69361
                     /locus_tag="VOX58_000064"
     CDS             68303..69361
                     /locus_tag="VOX58_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HES5877346.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            69374..70297
                     /locus_tag="VOX58_000065"
     CDS             69374..70297
                     /locus_tag="VOX58_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HES5877347.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     VVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPNNQDAGHQV
                     ADLLNKANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            70553..71266
                     /locus_tag="VOX58_000066"
     CDS             70553..71266
                     /locus_tag="VOX58_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HES5877348.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKTIAQRASQFIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGIDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            71263..72174
                     /gene="pfkB"
                     /locus_tag="VOX58_000067"
     CDS             71263..72174
                     /gene="pfkB"
                     /locus_tag="VOX58_000067"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HES5877349.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETEIN
                     GQGPVISREQLEDLKTKLSQLTAEDTVVFAGSAPANLGNAVYKELFPLVRQSGAQVVC
                     DFEGQTLIDALTYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            72171..74117
                     /locus_tag="VOX58_000068"
     CDS             72171..74117
                     /locus_tag="VOX58_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HES5877350.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            74210..74809
                     /locus_tag="VOX58_000069"
     CDS             74210..74809
                     /locus_tag="VOX58_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HES5877351.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYNTKLNELIEWYQLTNFDGLMK"
     gene            74961..75668
                     /locus_tag="VOX58_000070"
     CDS             74961..75668
                     /locus_tag="VOX58_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HES5877352.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
     gene            complement(75725..76042)
                     /locus_tag="VOX58_000071"
                     /pseudo
     CDS             complement(75725..76042)
                     /locus_tag="VOX58_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(76240..76584)
                     /locus_tag="VOX58_000072"
     CDS             complement(76240..76584)
                     /locus_tag="VOX58_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877353.1"
                     /translation="MLRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQEFDFKIRKF
                     LMESKEIHLTKSPYIRGSLEIHSKNRKHEKINLYDAKPNSTRSDVLKKYKDNKTINMK
                     DFSHFDIYLWTK"
     gene            complement(76625..77650)
                     /locus_tag="VOX58_000073"
     CDS             complement(76625..77650)
                     /locus_tag="VOX58_000073"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HES5877354.1"
                     /translation="MRKRCYSTSAAVLAAVTLFALSVDRGVIADSFSANQEIIYSEVT
                     PYHVTSVWTKGVIPPANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNM
                     LHWWFDQNKEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKA
                     FPGLSARRIGVMPDLALDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGD
                     QSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTCANVRINHVINLWGADFDSN
                     GNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQ
                     DSWNQTN"
     gene            78154..78356
                     /locus_tag="VOX58_000074"
                     /pseudo
     CDS             78154..78356
                     /locus_tag="VOX58_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            78558..78938
                     /locus_tag="VOX58_000075"
     CDS             78558..78938
                     /locus_tag="VOX58_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HES5877355.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            78938..79786
                     /locus_tag="VOX58_000076"
     CDS             78938..79786
                     /locus_tag="VOX58_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HES5877356.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            79911..81119
                     /locus_tag="VOX58_000077"
     CDS             79911..81119
                     /locus_tag="VOX58_000077"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HES5877357.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDH
                     IEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            81116..82993
                     /locus_tag="VOX58_000078"
     CDS             81116..82993
                     /locus_tag="VOX58_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HES5877358.1"
                     /translation="MSHFLVKKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDASSVLDTVLSSDVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDATNLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGSYSDYLDEKAFEKETVEADLAKAIVTEEVPLPPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(83091..83168)
                     /locus_tag="VOX58_000079"
                     /pseudo
     CDS             complement(83091..83168)
                     /locus_tag="VOX58_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            83885..84295
                     /locus_tag="VOX58_000080"
     CDS             83885..84295
                     /locus_tag="VOX58_000080"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HES5877359.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLSLTGLTAQICQHELDHLEGILI"
     gene            complement(84378..86390)
                     /locus_tag="VOX58_000081"
     CDS             complement(84378..86390)
                     /locus_tag="VOX58_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HES5877360.1"
                     /translation="MKKYFILKSSVLSILTSFTLLVTDVQADQVDVQFLGVNDFHGAL
                     DNTGTAYTPSGKIPNAGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANS
                     ALLQDEPTVKVFNKMKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEH
                     EASHQTIVIANVIDKKTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQ
                     NYEHYQFLDAAETIAKYAKELQEQHVHAIVVLAHIPATSKDGVVDHEMATVMEKVNQI
                     YPEHSIDIIFAGHNHQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFMTTPSAN
                     VIAVAPGIKTENSDIKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNL
                     VTTAQLTIAKKTFPTVDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQM
                     TGQHIYDVLNQQYDENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLT
                     TTYTVVVNDFLYGGGDGFSAFKKAKLIGAINTDTEAFITYITNLEASGKTVNATIKGV
                     KNYVTSNLESSTKVNSAGKHSIISKVFRNRDGNIVSSEIISDLLTSTENTNNSLGKKE
                     TTTNKNTISSSTLPITGDNYKMSPIMTILALISLGGLNAFIKKRKS"
     gene            86610..87260
                     /locus_tag="VOX58_000082"
     CDS             86610..87260
                     /locus_tag="VOX58_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HES5877361.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            87263..>87423
                     /locus_tag="VOX58_000083"
     CDS             87263..>87423
                     /locus_tag="VOX58_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5877362.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIM"
BASE COUNT        28194 a        14240 c        17530 g        27459 t
ORIGIN      
        1 ttgagtccac aactagtgac ttacctcgca gccttaaaac agattgcacc gcacgatata
       61 aaccaattat ttggggctat gtaccttcat ttgcaagatc ctaaattgga tctagtaaca
      121 tttaaacaaa ttgataacac attagtggag agtatctata aagcgttgac ctataaaggt
      181 attttttcag aagtcgaaaa agagcatcta tctactggtg cttaccaaac caaaaatgct
      241 ctttattcca atgacgaatt ggaaacactt ttaaattata acaagtacct ctatcttaaa
      301 gctgctaaac acattaaaaa gggacatttt ttgattaatc cttatacaag cgatggtaag
      361 acagttcaag gagatcaatt aaaagccatt acccgttttg aagcagatct tgacatggca
      421 caggcaaggc gactagtgac tctccctgct aaggagaaaa aagagtgctt tttaacatta
      481 atgagaaagg agagccactt gtgatttctt ttgccccatt tttaagcccc gaagctatta
      541 aacatttaca agaaaacgaa aggtgcagtg atcagtctca aaaacgcaca gctcaacaaa
      601 ttgaagcaat ttatactagt ggccaaaata tacttgtatc agcttctgct ggttcaggaa
      661 aaacctttgt aatggtcgaa cgcatacttg ataaaatttt gagaggtgtt tcaattgatc
      721 ggctttttat ctcaaccttt actgttaaag cagctacaga actgcgtgaa cggattgaaa
      781 acaaattata ctcacaaatt gctcaaacta cagattttca aatgaaagtt tatttaacag
      841 aacaattgca atctcttggt caagctgata ttggtactat ggatgctttt gcacagaaag
      901 tagtaagtcg ctatggttat agcattggca tttcatccca atttcgtatc atgcaggata
      961 aagcagaaca agatgtttta aagcaagagg tgtttagcaa actctttagt gagtttatga
     1021 atcaaaaaga ggcaccggtg tttagggctc ttgtgaaaaa tttttctggt aactgtaaag
     1081 acacttcagc ttttagagag ttagtttata cttgttattc ttttagccaa tcgacagaaa
     1141 atccaaaaat atggttgcaa gaaaattttc taagcgctgc taaaacttac caaaggcttg
     1201 aagatatccc ggatcatgat attgaactct tacttttggc aatgcaagac actgcaaatc
     1261 agctaagaga tgtgactgat atggaagatt atgggcagct gactaaggca ggtagccgat
     1321 ctgctaaata cactaaacac ttaacgatca tagaaaagtt gtctgattgg gtgcgtgatt
     1381 ttaaatgttt gtatggaaaa gccggattgg atcggttgat cagagatgtg acaggcctta
     1441 taccatctgg gaatgatgtt acagtctcga aggtaaaata ccctgttttt aagaccttgc
     1501 atcaaaaatt aaaacaattt aggcatttag aaacaatttt aatgtatcag aaagactgtt
     1561 ttcccttatt ggaacagtta caagattttg tgcttgcgtt ttcagaagct tatttagctg
     1621 tcaaaataca agaaagtgct tttgaatttt cagatattac acactttgca atcaaaattt
     1681 tagaggaaaa tacggatatt cgccaatcct atcagcaaca ctatcatgag gtgatggttg
     1741 atgaatatca agataacaat catatgcaag agcgactcct gaccttacta tcgaacggtc
     1801 ataatcgctt tatggtagga gatatcaaac aatcgatcta tcgatttcgg caagccgatc
     1861 ctcagatttt taatcaaaag tttagagact atcaaaaaaa acctgagcag gggaaagtga
     1921 ttttactcaa agaaaacttt cgtagccaat cagaggtgtt aaatgtcagc aatgctgttt
     1981 ttagtcactt aatggacgaa tcagtaggag acgtcttata cgatgagcaa catcagttaa
     2041 tagcaggtag tcatgctcaa acagtcccct atctagaccg tcgtgctcag ttattgctat
     2101 ataatagcga taaagatgat ggcaacgccc cttcagatag tgagggtatt tcatttagtg
     2161 aggttacaat tgttgccaaa gaaattatta agcttcacaa tgataagggt gtcccttttg
     2221 aagacattac gttactcgtt tcttcaagaa caagaaatga tatcatttct catacattca
     2281 atcaatatag tattcctata gtaacagatg gtgggcagca aaactatctt aaatctgttg
     2341 aagtgatggt tatgttagat acattacgca ccattaataa cccaagaaat gattatgccc
     2401 ttgtggcttt actgcgctca ccgatgtttg cctttgatga ggatgattta gcaagaatag
     2461 cacttcaaaa agacaatgag ctagataaag attgcctata tgacaagata caaagggctg
     2521 tgattggaag aggtgctcat cctgaattga ttcacgatac cttgcttggc aagttaaata
     2581 tttttttaaa gacgttgaaa agttggcgtc gatacgctaa gctagggtcg ttgtatgact
     2641 tgatttggaa aatttttaat gatcgttttt attttgattt tgtagctagt caagcaaaag
     2701 cagaacaagc acaagctaat ctatacgcat tagctctacg tgctaatcag tttgaaaaat
     2761 cgggctataa agggctatac cgttttatta aaatgattga taaggtactt gagacgcaaa
     2821 atgacttagc agatgtggaa gtggctgctc ctaaacaagc tgttaattta atgaccattc
     2881 acaagtctaa aggtttacaa tttccgtatg tatttatcct taattgtgac aagcgcttct
     2941 caatgacaga tattcataaa tcatttattc tgaatcggca gcacggtatc ggtatcaagt
     3001 accttgcaga tatcaaaggt ttacttggtg aaacaacact caattctgtt aaagtaagca
     3061 tggaaacctt accttatcaa ttgaacaaac aagagttgcg cttagcaact ttatcagaac
     3121 aaatgcgctt attgtatgtt gctatgacac gagctgaaaa aaaagtttat tttattggta
     3181 aagctagtaa gagcaaaagt caagatatca cagatcctaa aaagttaggc aaacttttgc
     3241 cgctggcttt acgagaacag ttattgacat tccaagattg gctattagca atagcagata
     3301 tattttcaac tgaagatctt tattttgatg ttcgctttat tgaagatagt gatttgacac
     3361 aagagtcagt cggacgactt caaacaccac agttattaaa tccagatgat cttaaagata
     3421 atcgtcaatc agaaacaatt gcacgggctt tagatatgtt agaagcagtg tctcaattga
     3481 atgccaatta tgaagcagct attcatttgc caacagttcg aacgcctagc caacttaagg
     3541 tagcttacga gcctttatta gaacccattg gtgtagatat tatagagaaa tcttctcgat
     3601 cgctatctga ttttactttg ccacattttt caaaaaaagt aaaagttgaa gcaagtcata
     3661 ttggatcagc tcttcatcag ttgatgcagg tgctcccttt gtcaaaaccg ataaatcaac
     3721 aaacgctttt agacgcttta agagaaattg atagtaacga agaggtaaaa acagctcttg
     3781 atctcaaaaa aatagagtcg ttcttttgtg atacaagcct aggccaattt tttcagactt
     3841 accaaaaaca cttgtatcga gaagcaccat ttgctatttt aaaagttgac cctattagtc
     3901 aagaagaata tgtcctacgt ggtattatag atgcctactt tttgtttgat gatcatattg
     3961 tattagtgga ctataaaaca gataaataca agcagcccat tgagttaaaa aagcgttacc
     4021 aacaacagtt ggagttatat gcagaagctc tcactcaaac gtataaactt cctgtgacta
     4081 agcgctatct tgttttaatg ggaggtggaa agccagaaat tgtcgaagtt taaggttgtt
     4141 gtgacacaag ggtattttgc aaatgataat agtatagttg aggattttac gaaatgcttg
     4201 aaaagctatt gacctttgaa taataataca atataataag ttttattagt gacaaaaagg
     4261 aataaatata ctatgaattt taaaaaaata agatttggtt ttttgctttg cctcctactt
     4321 tatcctttag cagcttgtaa taagtctgaa caacttaatc actacgaaag aattaaaaaa
     4381 acaagaaaat tagtcgttgc tgtaagccct gattatgctc cttttgaatt taaagcttta
     4441 gttaatggaa aagatactat tgttggagca gatgttcaat tagctcaggc tattgctgat
     4501 gaattagacg ttgatcttga gctttcacca atgagttttg ataatgtttt gtctagttta
     4561 cagactggta aagctgatct tgctatttca ggaatctcac atactaaaga aagagctaaa
     4621 gtatatgatt tttcaattcc ttactatcag gcagaaaacg ctatagtgat gagagcatct
     4681 gatgctaaag tgaccaaaaa tattagtgac ctaaacggta agaaagtagc cgctcaaaaa
     4741 ggtagtatcg aggaaggttt agttaaaata caattaccaa aggccaatct gatttctttg
     4801 actgctatgg gagaagccat taatgaacta aaagcaggac aagtctatgc agttacctta
     4861 gaagcacctg tagctgctgg ttttttagcc caacataagg atctggcttt agcacccttt
     4921 agcttaaaaa ccagtgatgg agatgccaaa gcagtggccc ttcctaaaaa tagtggtgac
     4981 ttaaccaaag ccgttaataa ggttattgct aagttagatg agcaagaacg gtacaagtca
     5041 tttattgcag agacaatagc actaacgaaa aatactatga agtaataagg ttaataaaaa
     5101 ctttattagt atccgttgga aaagtgttta gaataaacaa taaaagaggt ttgacttttt
     5161 ttaaaaaata atgtaatata attgggaacg tgaaaatgtt acaatatctt gaggaggtga
     5221 aacacatgtc aaaaacagta gtacgtaaga atgaatctct tgacgatgct cttcgtcgct
     5281 tcaaacgttc tgttactaaa gctggtactc ttcaagaatc acgtaaacgt gaattctacg
     5341 aaaaaccttc tgtaaaacgt aaacgtaaat cagaagcagc tcgcaaacgt aaaaaattct
     5401 aatggtaaaa aataaaaaca gttctcacaa ttagaactgt ttttattttt tatccagaag
     5461 aagcaaggga gcttttttga tatcaaaaaa agcagttaga catgcctaac tactttatca
     5521 aaaatcgctt tatttttgag ccaacaaatc acggatttct gtcaataatt cttcttgagt
     5581 aggagctgca gcttcttttt cttttttagg catagctttt tcagcggctt ttacaacaaa
     5641 gaaaagactg gtaccgatga taagaaaatt aataacggct cctaaaaagc taccatattt
     5701 aacaccattc catgacaatt gagtgatgtt ttcaacattt gcggccttca gagctgggtt
     5761 taaaataagc ggtgtgataa tatcgttcac aaaagaagtg acaatagcac caaaagctcc
     5821 cccgatgata actgctacag caagttcaat aatgtttcct ctaaacaaaa atgcttttaa
     5881 ttctttaacc attaaatatt ccccctttta atatctctaa gttaagcata gcataattag
     5941 taaacaaaat ccaatggaat cacttcagtt tttaaattta caaaaaagag tttcggtggt
     6001 ataataaaac gtaaaactag aatgggattt ttatggggag gtgacgattt ggcaattgac
     6061 aaagaaatga tttcccaagt aaaaaatagc gttaatattg tcgatgtcat tggagaagtg
     6121 gtcaaacttt cccgatcagg gcggcattat ctcgggcttt gcccatttca taaggaaaag
     6181 acaccctctt tcaatgttgt tgaagacaga caattttttc actgctttgg ctgtggaaaa
     6241 tcaggggatg tttttaaatt tattgaggaa taccgccaag tccccttctt agaaagtgtt
     6301 cagattattg ccgataagac tggtatgtcg cttaatatac cgccaagtca ggcagtactt
     6361 gctagccaac acaagcaccc taatcacgct ttgatgacac ttcatgagga tgctgctaaa
     6421 ttttaccatg cagttttgat gaccactacc attggtcaag aagctaggaa gtacctttac
     6481 cagagaggct tggatgacca attaattgag catttcaata ttggtttagc cccagatgag
     6541 tcagattatc tttatcaagc tctttctaaa aaatacgagg aaggtcaatt ggttgcttca
     6601 ggattgttta acttgtccga tcaatccaat accatttacg acgcctttcg aaatcgtatc
     6661 atgtttccct tatcagatga ccgagggcat attattgcct tttcaggacg tatctggacg
     6721 gcagctgata tggaaaagag acaggccaag tataaaaatt caagaggaac agttcttttt
     6781 aacaaatctt atgaattgta tcatctggac aaagcaaggc ctgttattgc caaaacccat
     6841 gaagtgtttc taatggaagg gtttatggac gtgattgccg cttaccgttc cggctatgaa
     6901 aatgctgttg cttcaatggg gacggcattg actcaagaac atgtcaatca ccttaagcaa
     6961 gtcactaaaa aagttgtttt gatttatgat ggtgacgatg ctggacaaca tgctattgca
     7021 aaatcactag aattgcttaa agattttgtt gtcgaaattg tcagaatccc caataaaatg
     7081 gatcctgacg aatttgtaca acggcattcc ccagaagcat ttgcagattt gcttaagcag
     7141 tcacggatca gtagtgttga attttttatt gattacctaa aacctactaa tgtagacaat
     7201 ctgcaatcac aaattgttta tgtggagaaa atggcaccat tgattgctca atcaccatcc
     7261 atcacagctc aacattcgta tattaacaag attgctgatt tgttgccaaa ctttgactat
     7321 tttcaagtag aacaatcagt aaatgcatta aggattcaag ataggcaaaa gcgtcaaggt
     7381 caaatagctc aagccgtcag caatcttgtg accttaccaa taccaaaaag tttgacagct
     7441 attgctaaga cagaaagtca tctcatgcat cggctcttac atcatgacta tttattaaat
     7501 gaatttcgac atcgtgatga tttttatttt gatacctcta ccttagaaat actttatcaa
     7561 cggctgaagc aacaaggata cattacatct tatgatttgt cagagatgtc agaggaagtt
     7621 aaccgtgctt attacaatgt tttagaagaa aaccttccca aagaagtagc tcttggtgag
     7681 attgatgata ttttatccaa acgtgccaaa cttttagcag agcgcgatct tcacaaacaa
     7741 gggaaaaaag ttagagaatc tagtaacaaa ggcgatcatc aagcggctct agaagtacta
     7801 gaacatttta ttgcgcagaa acgaaaaatg gaatagagga aattatgaca aaacaaaaag
     7861 aaataacaac ttttaacgtt caagttgcgg agtttattcg ccatcacaaa aaagaaggaa
     7921 cagcgattga tgatgacgtg accgaaaaac ttgtcattcc atttgctttg gatgctgatc
     7981 agattgatga tttacttgag cggttaacag atggtggtat ctcgattacg gataaagaag
     8041 gtaacccatc atcaaaatac attgttgaag aaccaaaacc agaagaatta actgatgaag
     8101 aattaattgg tagtaattca gctaaagtaa acgaccctgt ccgcatgtac ctcaaagaaa
     8161 ttggtgtggt accgctttta acaagtgaag aggaaaaaga attggctgtt gcagttgcca
     8221 agggagattt aatggctaaa caacgtttag cggaagcaaa ccttcgtctt gttgtctcaa
     8281 ttgcaaaacg ttatgttgga cgtggaatgc aatttcttga tttaattcaa gaaggtaaca
     8341 tgggactcat gaaagctgtt gataaatttg attattcaaa aggattcaaa ttctcaactt
     8401 atgcaacatg gtggattcgt caagctataa cacgcgcaat tgcagatcaa gcacgtacga
     8461 ttcgtatccc tgttcatatg gttgaaacga ttaataaatt agtccgtgaa caacgtaacc
     8521 ttcttcaaga acttggtcaa gatccaacgc cagaacaaat cgcagaacgt atggaaatga
     8581 ctccagacaa ggttcgtgaa attctaaaaa ttgctcaaga acctgtttct ttagaaacac
     8641 caattgggga agaagatgat agtcacttag gtgattttat cgaagatgaa gttattgaaa
     8701 atccagtaga ttatacgacg cgtgttgttc ttcgtgagca attggatgaa gttttagata
     8761 cgcttactga ccgtgaagag aacgttcttc gcctacgctt tggccttgac gatggtaaaa
     8821 tgcgtacgtt agaggacgta ggtaaagtct ttaatgttac tcgtgagcgc attcgacaga
     8881 ttgaagctaa agcccttaga aaacttcgcc atcctagtcg tagtaaacaa ttaagagact
     8941 ttatagagga ctaaaatcgt ccagtggacg attttaggtc gttcctttga aataagaaag
     9001 gagcgaaaat cctaagtggc acttatcagc ggtacaccta caaattagaa atcggaccga
     9061 atgtggtggg tatttttaag gttggacttg atatcttaaa caagcccaga gggcaattcc
     9121 tctaaatagg caaagcttta gtggtttttt tacttatcta cgacatattc aaaaaatgaa
     9181 aggaaagcca tgtctgatac accaaaatat acacaagatc aggtgatagc tatcaagaat
     9241 agaattcttg aagctttaga aactgttatt gatcccgagc taggtattga tgttgttaat
     9301 ctaggtttga tttatgaaat tcggttcaat gataacggtt atacagagat tgatatgaca
     9361 ctcacaacaa tgggttgtcc tttggcagac ttgttgacag attacatcca tgatgccctg
     9421 caagatgtgc cagaagtcac taagactgag gtcaaattag tctggtaccc tgcttggacg
     9481 gttgataaaa tgagccgcta tgctaggatt gcgctgggta ttcgctaatc attgatagta
     9541 aagggatgaa atattccctt ttttgatgta gaataaaagg aaatacaggt gaaagccttt
     9601 ggttaatgtt agagaacact aagatactga gaaaagtgtt ctacctatgg caaaaaggag
     9661 aactgatgat tttaattaca ggaagcaatg gtcaattagg aacagaactt cgatacttat
     9721 tagatgaacg aggtgttgat tatgtagccg ttgatgtcgc agaaatggac atcacaaatg
     9781 aagacaaagt cgaagctgtt tttgcgcagg tcaagccaac attggtctat cattgtgctg
     9841 cttatacggc agttgatgca gcagaagacg aaggaaaagc cttgaatgag gctattaatg
     9901 taacaggctc agaaaatatt gccaaggctt gtggaaaata tggggctacc cttgtttaca
     9961 tttcaacaga ctacgtattt gatggcaata aaccagtcgg tcaagaatgg gtagaaacag
    10021 atcgtccaga ccctaagaca gaatatggcc gtacaaaacg tttaggagaa ctagctgttg
    10081 aacgatacgc agagcatttt tatattatcc gtactgcttg ggtatttgga aattatggta
    10141 aaaactttgt ctttgccatg gagcagttag cggaaaatca ctcccgtttg actgttgtca
    10201 atgaccaaca cggtcggcca acgtggacaa gaaccttggc agaattcatg tgttatttga
    10261 cagagaatca aaaagccttt ggttactatc acttgtcaaa tgatgctaag gaagacacca
    10321 cttggtatga ttttgccaaa gaaatcttaa aagacaaggc tgttgaagtg gtgccagttg
    10381 attcatcagc tttcccagca aaagccaaac gtcctttaaa ttcaaccatg aatcttgata
    10441 aggcaaaagc aacagggttt gttattccaa catggcaaga agccttgaag gcattttacc
    10501 aacaaggact gaaaaagtaa gtattctaaa aagagttggc tttctaaggc caactctttt
    10561 tcatgggttt aaggtcgcat tagccttcta attgtggtat aatatcactg attatatttt
    10621 agaatggggt agtatgcaat gcaggatgtt tttatcattg gtagcagagg gttaccagcg
    10681 aaatacggtg gctttgaaac ctttgtcgaa gagttgatta gtcatcaatc atctaaaaac
    10741 attcgttatc acgttgcttg tttaagcgat acgaagcata aggttcattt tgattataag
    10801 ggggcagatt gcttttacct taatccccca aaacttggtc ctgctagagt tattgcttat
    10861 gatatgatgg caattaccta tgccctgtct tatagcgacc aacaccagat tcagaaccct
    10921 attttttatg tgttgggcaa tactgtaggc gcttttattg cgccatttgt gaagcagatt
    10981 cacaatcgag gtggacgctt ttttatcaat ccagacgggt tagagtggaa acggtctaaa
    11041 tggtcaaaac cagtgcaggc ctacctgaag ttttccgaaa agcaaatgac tagacaggct
    11101 gatttggtca tttcagataa tattggcatt gaccgctacc ttaaacaggt ttatccttgg
    11161 tctaaaacat gttttattgc ttatggaacg cagacacaac cttcacgttt gacaacagca
    11221 gatagcaagg tcagagctta ttttcaaacc tttgatatta gagaaaagga ttactatctc
    11281 attttaggac gctttgttcc tgaaaataat tacgaaactg ctattaaaga atttatggct
    11341 tcctcaacta agcgtgattt agtgattatt tgtaatcatg aagacaatgc ttactttaag
    11401 caattacttg ctgagacaga gtgtgataaa gacccacgca ttaaatttgt tggtaccctt
    11461 tatgacaaag aactattagc ctacattcga gaacaggctt atgcctatat ccatggtcat
    11521 gaggtaggag ggactaatcc aggccttcta gaagcactag cccataccaa tcttaatttg
    11581 gttttaggag ttgattttaa ccagtcggtg gctaagtctg ctgcgctgta ttggtccaag
    11641 caaaaagggc aattagcaga gctgattaac caagttgatg caggttttga tagtgatcgt
    11701 ctgggcaaag aagccaaagc tattattcaa gaacattaca cttgggaaaa aattgtaggg
    11761 gagtacgagg ctttattttt aaatgaacat taatatttta ctatccacct acaatggaga
    11821 acgcttctta gcagaacaaa ttcaaagtat ccaaaggcaa acagttaacg actggacctt
    11881 attgattaga gatgatggat caactgatgg cacccaggat attattcgca catttgtcaa
    11941 ggaagacaaa cgaattcagt ggatcaatga ggggcagaca gaaaatctgg gagttattaa
    12001 gaatttttac accctcttaa agcaccaaaa agcagatgtc tattttttca gtgaccaaga
    12061 tgatatatgg ttagacaata aactggaagt gaccttattg gaggctcaaa aacatgaaat
    12121 gacagctcct ttattggttt atacagacct caaagtggta acccaacatt tagctatttg
    12181 ccatgacagt atgattaaga cccaatctgg tcatgcaaac acaagcctac ttcaagaatt
    12241 aaccgaaaat accgtaacag gcggcacaat gatgattact catgctctgg ctgaggaatg
    12301 gaccacttgt gacggtttat tgatgcatga ctggtattta gctttactag cttctgctac
    12361 tgggaaatta gtttaccttg atattccaac agaattatac cgtcagcacg atgctaacgt
    12421 gttgggagct agaacttggt ctaaacgcat gaaaaattgg ttaacccccc atcatttagt
    12481 caacaaatat tggtggttga ttacttcaag ccaaaaacag gctcaattac tattagattt
    12541 accattaaaa ccaaatgacc atgaattggt aacggcctat gtttctttat tggatatgcc
    12601 ttttaccaaa cgcttagcta ccttgaaaag gtacggtttt agaaaaaatc gcatctttca
    12661 cacctttatt tttcgtagtt tagttgttac cttatttggt tacaggagaa agtaagaatg
    12721 aattttttaa caaagaaaaa ccgtatttta ctgagagaaa tggtaaaaac ggattttaaa
    12781 ttacgctatc aagggagcgc aattggctat ctatggtcta tcttaaaacc gcttatgatg
    12841 tttacgatta tgtacttggt atttattcgt tttcttcgcc tgggtggaaa tgtacctcat
    12901 tttccagtag cgcttttatt ggcaaatgtc atctggtctt ttttttcaga agcaactagc
    12961 atgggaatgg tatctattgt atctcgggga gacttgttgc gaaaattgaa cttttctaag
    13021 cacatcattg ttttttcggc agtgttagga gctttaatta attttcttat taatttggtt
    13081 gttgttttaa tttttgcgtt gataaatggt gtgactatat cagggtatgc ttatctctct
    13141 ctttttcttt ttatagaatt agttgtttta gtgcttggaa ttgcattgtt attgtcgaat
    13201 gtctttgttt attatcgtga cttagctcaa gtctgggaag tactattaca agcaggtatg
    13261 tatgccactc caatcattta tccgatcact tttgttttag atagccaccc tttggcggca
    13321 aagttgttga tgctaaatcc agtagcacaa atgattcaag attttcgtta tttattgatt
    13381 gacagagcca acgtaacgat ttggcagatg tcaaccaatt ggttttacgt tgttattcca
    13441 tatttagtac catttgttat attatttatt ggcatctttg tctttaagaa aaatgccgat
    13501 agatttgcgg agattattta atgattaata gagaaattgc agtaaaagta cagcatgtta
    13561 gtaaaacatt taaactgcct acagaagcaa ctaaaagttt tcgcacgact ctggttaatc
    13621 gcctaagagg gattaaagga tttactgaac aacatgtgct aaaagatatt aactttgatg
    13681 tttataaagg cgattttttt ggaattgttg ggcgtaacgg ctcgggaaaa tcaacgctcc
    13741 ttaaaattat ttcccagatc tatgttccag aaaaaggaca ggttacagta gatgggaaga
    13801 tggtttcttt tatagagctt ggagtgggat tcaatccaga attaacagga cgagaaaacg
    13861 tctatatgaa cggcgccatg ctgggattta ccaaagacga agttgatgat atgtacaatg
    13921 atattgttga ttttgcagag ttgcatcatt ttatgaatca aaaattaaaa aattattctt
    13981 ctggcatgca ggtacgttta gcattttcag tggctattaa agcccaaggt gacgttctta
    14041 ttttagatga ggtgcttgct gtaggagatg aagcttttca gcgaaaatgt aatgattatt
    14101 ttatggaaag aaaagacagt ggtaagacga ctattttagt gacacatgat atgggagctg
    14161 ttaagaagta ttgtaaccgt gcagttctta ttgaagatgg tcttgttaaa gcatatggag
    14221 aaccatttga tgttgctaat cagtatagtg tagataatac agagacagct gaggatgcaa
    14281 tgaatgctga aaaaatatct gtatctgata ttgccaaaga tttgaaagtg tcattaatct
    14341 caaatccaag aataacacca aatgacacaa tcacatttga agtttcttat gaagttttaa
    14401 aagatgattt tacctacatt gccttttctt taacggatat tgatagaaac atttgggttt
    14461 acaacgacaa ttctttagat tatccaacac aaggtatagg tagaaaatgc atttcctacc
    14521 aatgtcaact atctcaactt aatgatataa aactaaaatt agaagtaact gtccgagata
    14581 agaacggtca gatgctactc ttttcaacag cagaacaatc accccagata gtgattcaaa
    14641 gaaacgatat tgatcaagat gatctgtcag ctctagattc agcaagtggc ttgtatcaac
    14701 gtaatgggaa gtgggttttt cattaatgag taatagctag aagggaattt atgtataaag
    14761 tttcaattat ttgcaccaat tataacaaag ctccttggat atctgatgca cttgacagtt
    14821 ttttaagcca agtgacagat tttgaggttg aaattattgt tattgatgat gcttctacag
    14881 atgactcacg agagatttta aagagttatc aaaaaaagtc ttcaggaaaa attaaattac
    14941 tctttaacga aactaatatt ggaataacaa agacttggat aaaagcatgc ctttatgcaa
    15001 aaggcaaata tattgctaga tgtgatggtg atgattactg gacggatagt tttaaactcc
    15061 aaaaacaagt tgatgtctta gaagcttcca aacggtcacg atggtgtaat actgattttg
    15121 actttgtaaa tagtcaaggc gacttattat atgctaatgc tttttcatct ggtcatacac
    15181 ctttgacaga tacttacgaa aaagttttag ccttaaaagg gatgacgatg gcatcgacat
    15241 ggttagttga tgctgattta atgagacaag taaaccaaaa aatcaatgta gatacaccag
    15301 acgatacttt tgacattcaa ctagagttat ttcaactgac tcaactgacc tatattaaag
    15361 actcaacgac ggtttatcgc atgacaaccg attctgattc aagacctact gatacaccaa
    15421 aaatgattca tcgtattcaa aaactacttg atactcagtt aaattactta aaaaagtata
    15481 atcaggtaga tacaaaagaa atgtctgaac tattattaca acaagatgct aagcaagaaa
    15541 tacgaattca tgagttgagt tgctttattc aagaattaca acaaactatc gtagataaaa
    15601 caaagcagca agaaacacgt gaagtggagt tgcaaaatgt catagaagaa caaaaaaatc
    15661 aactatctga attaagacaa caataccatg ccattattaa ttcacgtcaa tggaaataca
    15721 cgtctaagct cattgctttt attaggagaa aaaaatgaat cgtattttat tatatgttca
    15781 cttcaacaaa tacaataaaa taagtgcaca tgtttactat caattggaac aaatgagatc
    15841 actgttttct aagattgttt ttatctcaaa tagtaaagtg agtcatgaag atttaaaacg
    15901 tctcaaaaac cactgtctta ttgatgagtt tttgcaacgc aaaaataaag gttttgattt
    15961 ttctgcatgg cacgatggct tgattactat gggatttgac aagttggaag agtttgactc
    16021 attgacgatt atgaacgata cttgttttgg tcctatttgg gagatgggtc cttattttga
    16081 aaattttgaa gaaaaagagg cagttgactt ttggggaatt actaataata gaggtacaaa
    16141 agcttttaaa gaacatgtcc aaagttattt tatgacgttt aaaaatcaag tcattcaaaa
    16201 caaagttttt caacaatttt ggcaatcgat aatagaatat gaaaacgttc aagaagtgat
    16261 tcagcattat gaaacgcaat taacatccat tctcttaaat gaaggcttta gttatcagac
    16321 ggtctttgat actagaaaag ctgagtcgtc attcatgcca catccggatt tttcatatta
    16381 taatccaaca gcaattctaa aacaccatgt gccttttata aaagtaaaag ctattgatgc
    16441 taaccagcat atcgctcctt acttactgaa tctgatacga gaaacaacca attatcctat
    16501 agatcttatt gtttcacata tgtcgcaaat ctctttgcca gatgctaagt accttttatc
    16561 tcaaaaatac ctaaactgcc aacggttagc aaaacaaact tgtcaaaagg tagctgttca
    16621 tttacatgtt ttttacgttg atttgctaga tgaattttta acagctttcg aagactggaa
    16681 cttccattat gatcttttta ttacaacaga tagtgatatc aaaagaaaag agatcaaaga
    16741 aatacttcaa agaaaaggta aaactgctga tatacgcgtt acgggaaata gaggaagaga
    16801 tatttatcca atgttattac taaaagataa actttctcaa tatgattata ttggtcattt
    16861 ccatacgaaa aaatcaaaag aagctgattt ttgggcagga gaatcttggc gaaaagaatt
    16921 gattgatatg ctcgtgaaac cagcagatag tattcttagc gtttttgaga cagatgatat
    16981 tggtattata atcgcagata ttccaagttt ttttagattt aataagattg tcaatgcttg
    17041 gaatgagcat ttaatcgctc aagaaatgat gtctttgtgg agaaaaatgg atgttaaaaa
    17101 acaaattgat tttcaagcta tggatacatt tgttatgagt tatggaacat ttgtatggtt
    17161 taaatacgat gctctaaaat cactttttga tttagaatta actcaaaatg atattccaag
    17221 tgaaccttta ccacaaaact ctatattaca tgcgattgag cggttatttg tctatatagc
    17281 gtgggggaat tcttatgatt ttaggattgt taaaaatccc tatgagttaa caccttttat
    17341 tgacaataaa ctgttaaatt tacgtgaaga tgaaggagct catacctacg ttaactttaa
    17401 tcaaatgggc ggtatcaaag gagcattaaa atatattatc gttggtcccg ctaaagcaat
    17461 gaagtatata ttcttgcgtt taatggagaa acttaaatga ttaaagacac atttttaaaa
    17521 accaattggt taaatattag tcaccatatt atccttcttg ttttcggttt ttatttcagt
    17581 ttttacagtt tggcgaaaga actagtaagc tccacggcac aaccggtaaa ctattatgct
    17641 catttactaa atgtttcttt tgtgggatat attatatcac tgattggatt atcttattat
    17701 ttgagtcgcc aagttagtcg acagttgttt ttgaaaacta gttttattgt gatatcttat
    17761 ctaattgtca gctattgggt acaaataaca cagcacctga atgataaacg gtttgatatc
    17821 tggtcattaa ctaaaaatca attttatcaa tttcaagctc taccttcttt actcattatt
    17881 ttagtgatgg ccactttaat aaaaatattg gtagcatatt ttgcaataga aaaagataga
    17941 tttgggctat taggctatca aggtaatact ttttctgtag ctctgatttt agcagttgtg
    18001 ccaattaatg acatacatct gttaaaacta ataagttctc gattttctga attagtaaga
    18061 gcaggtaata gccaaattgc actgttaaaa ataagtggac tgttgatagt tttacttgtc
    18121 atatttgcaa caatcatata cgtggtttta aatgctctaa aacaccttaa gtcaaataaa
    18181 ccttcatttt cagtagcagc tactactagt ttgtttttag cattagtttt taactatacg
    18241 tttcagtatg gcgtaaaagg tgatgaagca ttgctaggat attatgtttt ccctggagct
    18301 actctttttc agatagtagc tattacacta gttgctcttt tagcatacgt gataacgaat
    18361 agatattggc caactacctt ctttttgctt attctgggaa caattatttc tgttgttaat
    18421 gatttaaaag aatcaatgag aagcgagccg ttattagtaa ctgattttgt ttggttacaa
    18481 gaattaggtt tagtgacaag ctttgttaaa aaatcggtga ttgtagaaat ggttgtagga
    18541 cttgctattt gtattgtggt agcttggtat ctacatggcc gagttttagc agggaaatta
    18601 tttatgagcc ctgtcaaacg ggcaagtgct gtattaggtt tatttattgt atcttgtagt
    18661 atgttaatac cattttctta tgaaaaagaa ggtaaaatat tatctggtct tccgattatt
    18721 tcggctttaa ataatgataa tgatataaac tggttaggtt tttcaacaaa tgctaggtac
    18781 aaatctttag catatgtttg gacaagacag gtgaccaaga aaataatgga aaaaccgaca
    18841 aattatagcc aagaaacaat agcgagtatc gctcagaagt accaaaaatt agcagaagat
    18901 attaataaag acagaaaaaa taatattgct gaccaaacgg ttatttatct tttaagtgaa
    18961 agtttgtcag atcctgatag agtatcaaat gttactgtta gccacgatgt tttacctaat
    19021 atcaaggcaa tcaaaaatag cacaactgcg ggactcatgc agtcagactc ctacgggggt
    19081 ggaactgcta acatggagtt tcaaacgtta acaagcttac ctttttataa tttttcttct
    19141 tcagtatctg ttctttattc agaagtcttt cctaaaatgg ccaaacctca tacgattagt
    19201 gagttttacc aaggaaaaaa tcgtattgcg atgcatcctg ctagtgctaa caattttaat
    19261 agaaaaacag tttatagtaa tttaggtttt tccaaattct tagctctatc gggttctaag
    19321 gataagttta agaacattga aaatgtcggt ttattgacta gcgataaaac tgtctataat
    19381 aatattttat ctttaattaa tcctagtgaa agccaatttt tctcagttat tacaatgcaa
    19441 aatcatattc cttggtcatc cgattatcct gaagaaattg ttgctgaagg aaaaaatttc
    19501 acggaagaag aaaatcacaa cctaacaagt tatgctcggt tattatcgtt tactgataag
    19561 gaaacaagag catttttaga aaaattaaca caaattaaca agcctatcac agtggtgttt
    19621 tacggagatc atttacccgg tttatatcct gatagtgctt ttaacaagca tattgaaaat
    19681 aaatacctta ctgattattt tatttggagt aatggtacta acgagaaaaa aaatcatccg
    19741 cttatcaact caagtgattt tactgcagct ttatttgagc atactgattc aaaagtatca
    19801 ccttactatg ctttgttaac agaggtactg aataaagcta gtgtcgataa atcaccagat
    19861 agtcctgaag ttaaagctat tcagaatgat ttaaaaaata tccaatacga tgtgactata
    19921 ggaaaaggtt accttttgaa acacaaaact ttttttaaga tatcacgtta agaaaaatgc
    19981 taggctgtcc gagtatagac gagcatcaag gtttggtaat ttgctcaaat gattgtcaac
    20041 agagtgtcta ggaccaggtt taaaagatta taattcttaa ggtcttattc gtttataaaa
    20101 aatgaatact cttttaaaat cttaattgaa atgaatagga gaagttttcg tgaaaaagtt
    20161 aatcattatt cctgcttaca atgaaagcag taatattgtc aatactatac gtactattga
    20221 atcagatgcc ccggattttg actatatcat tattgatgat tgctcaacgg ataatacgtt
    20281 agcaatatgt caaaaacagg ggttcaatgt tatttctttg cccattaacc tgggaattgg
    20341 cggtgcggtg caaactggct atcgttatgc acaaagatgt ggatatgacg ttgcagttca
    20401 agtagatgga gatggtcagc acaatccatg ctatttggaa aaaatggttg aggtattagt
    20461 tcaatcttca gtaaatatgg taattggatc acgatttatc acaaaagaag ggtttcagtc
    20521 atcatttgct cgacgtatcg gcataaagta ttttacttgg cttattgcgc tactaacagg
    20581 aaaaaaaata acagatgcaa cgtcaggtct aaggttaatt gaccgttcat tgattgaacg
    20641 ttttgctaat cattatccag atgattatcc tgaacctgaa acagttgttg atgtattggt
    20701 tagtcatttt aaagtgaaag aaattcctgt tgtaatgaat gagcgacaag gcggtgtgtc
    20761 atctatctcg ttgacgaaat cggtctatta tatgattaaa gtgactttag ctattttagt
    20821 tgttagattg aaaggaaatc gttaaaatga cggtaacagc cagtttattt ataagtttta
    20881 ttgtattaac ctttgttttc tttttgatta acttaatcaa aaaagataaa ctagcgatta
    20941 agtactcttt attgtggttt attttagctt tattaatttt actatttacg tggttaccta
    21001 acatattaaa taaaatgtct cacttcctgg gtattcattc accaaccaat atgttattct
    21061 ttttaggttt ttgtttgagt ttagctatta ttttttcact gactaataat atttcacttc
    21121 aaaatgataa agtaaaacgt ctgacccaag aagtggccct tatgaaaaaa gagaaaacca
    21181 atgattaatc cttcaaaaca aaaccaaacg atttttttat ggaatatgtt aggaagttta
    21241 tcaacagctg taatatcagt tatattactt atggtggtta ctaggttact aacttcggct
    21301 gattcagata tttacgcctt tgcctattct tttgctaata tgatggtagt tgttggactt
    21361 tttcaggttc gtaattatca agcaacagat attaatgaaa aatattcctt tagtcagtac
    21421 ttagtggcaa gactgatgac ttgtttatta atgttagcca taacagtaat ttatttaacg
    21481 ttaactaaga cagatagcta caagagtaca attgtctttc tagtctgttt ctatagatcg
    21541 acagatgctt tttcggattt atatcaggga atgttccaac aacatgaacg gctggatatt
    21601 gcaggtaaat cgctagccta cagaaatact cttattttta tggtgtacac agcaataata
    21661 ttatattcca aaaatcttac tttagcatta gtagctgtct gtatagtttc attagttttt
    21721 ataatgtatt atgacattgg tcattcgaaa aaatttcaaa agttgatgtt tagtgaatta
    21781 ttaagtaata tttcatttca aaacagttta aagttattaa aagaaagttt tccacttttt
    21841 ctaaacggtt ttttgattat ctatatttat actcaaccaa aatacgctat tgaactaatg
    21901 acgactttag gtgaagttgc tttgggctct caaacaattt ttaatatttt atttatgcca
    21961 gcctttgtta tgaatttgct aattttattt tttagacctc atattacaca gatggctatt
    22021 gctttaatca gaggtcaaat aaaagagttt aataagatac aagttcaatt gtttgcttat
    22081 ttaggagttt tttcactgat agcgttagtt ggaagtggtt tgtttggtat tcccttttta
    22141 tcaatactat atggcactaa ccttacggat tattgggtag attttatgtt gattatgcta
    22201 ggaggctcga ttggtagttt tgcgacagtt atcgataata tcttaaccgc tatgagaaaa
    22261 cagcaacttc ttcttattcc atatacagga ggatttttga tttcgttatt aattaccaat
    22321 ctttttgtta tgaaatatca tattttagga gctgctttga gctttttaat aacaatgttg
    22381 gtttggttgg gattatccat tatgatttat ctatttatta tgaatagatt taagaaggga
    22441 agagttaatg caacaatcta tgactaaagt taaatatctt tggaaagaaa ataataacta
    22501 tatttattcg acttttttga cattacttct gacagttatt ttatatgcca atagtttaaa
    22561 tctaccttta aaatttatta gtcttctttt tgtggtgagc actttactga tagtgttttt
    22621 accaaaaaag ttagaggttg ctacgatcat ttttatttta gtatttggaa cgatatctgc
    22681 tatcatatca cctataaatg atattcctga tgaatatgtt cattattcaa ggacagttta
    22741 tatctcagaa ggtgatatta atcttaccaa caataacaaa aagttaagaa tatccaaaga
    22801 tgttgataag cttattaaac agtcaggaaa aacatttata acatcaaact tgaaagcaac
    22861 taaacacagc acaagagagt atagctatcc ttacatcaaa ggaactaatg catattatag
    22921 tttttcttat ataccacaag ctttgggtat tttagtagga aatgcgctgg atttacctat
    22981 acttttaaca tattatttcg gaagattgtg caaccttatt agctacgcta tgcttgcttt
    23041 tatagctatc aagttgtctg gttcttttaa acaagtgata gctgttgtta ctttactacc
    23101 tatgaacatt tatttagcag catcttttaa tcaagacggc tttgctatcg gtttagtttt
    23161 agtaacaatt ggattgttta ttaacttgct ctcatcaaaa gataaatcta actataatac
    23221 caaattcttc ctttatttag ttctatgtgg gttactagtt ctttcaaaat tcacatactt
    23281 tttattagta tgccttccat tatttattcc taatgaaaaa ttcggaaaaa ataccaagtt
    23341 agtcatcttg aaaaaattgg gaggactatt actcattttt ctttttgctg caatgtggtt
    23401 tagactttat ggacaggtta aaaccccata tgtcgcggat tttctaaaag aagtaaacgt
    23461 tagccagcaa gttaaaaata tgctagaatc ccctatagtc tatagcagta ttattattag
    23521 acatatggta attaatctga ttaatatgaa taatattttt cagtttggcg cattaagcta
    23581 tggtataacg aaccttttcc cgctttatgt gtgtttcttc ttttttgttt atattagtaa
    23641 tgctagtaaa ataactatta atattgtaga aaaaatgggt ataatctttg ttatttcagc
    23701 gattataggt gcaactgtat tagctatgta cctgacttgg acaccagtag ggtcctcaac
    23761 tgtcttaggt gttcaaagtc gttatctgat aggaattatc ccattagtgt tgcttctttt
    23821 tagtagtcaa cagcaaaagt tcaaacaaat agaggatatc ctttctgaca aactggctat
    23881 tcatgtttcg cttttgttta ttctcgcaat gttaatgagt actattttta gatattatca
    23941 ttaaactctt aattagaggc tttaaaaaac ttagcttgaa taagtatttt aaagcctttt
    24001 atgctataat taaattgaga aattattgag gtggcctatg aagtacgata acttgttaga
    24061 tcgatttata aaatatgtca aagtgaatac aagaagcgat ccagacagtg agactactcc
    24121 tagtacggag agtcaagaag catttgcctt aacgatttta aagcctgaaa tggaggcaat
    24181 tggtttgcaa gatgttcatt ataatccagt taatggctat cttattggaa ctctgcctgc
    24241 caataatcca accttgacgc gtaaaattgg ttttatagca catatggata cggctgattt
    24301 caatgctgaa aatgttaatc cgcagattat tgacaattat caaggagggg acattacact
    24361 tggcagttct aattataagt tggatccaaa agcatttcca aatcttaata attatatcgg
    24421 tcaaacattg attaccactg atgggactac acttttaggg gcagatgata aatctggtat
    24481 tgctgaaatc atgacagcaa tagaattctt aacatcacaa ccacaaatag agcactgtga
    24541 cattaaagtg gcttttggcc ctgatgaaga gattggggtc ggagctgata agtttgaggt
    24601 ggctgatttt gaggtggatt ttgcttatac tatggatggc ggtcctttag gcgaattgca
    24661 atatgaaact ttcagcgctg cagctctaga agtgactttt ttaggcagaa atgttcatcc
    24721 tggtacggct aaggatcaaa tgattaatgc ccttgagtta gcaatagatt ttcatgagaa
    24781 gctacctgct aaagagcgac ctgaatacac agatggttat caaggcttct atcacttaac
    24841 cggactaact ggaacagttg aagaggcacg cgcatcttac attattcgtg attttgaaga
    24901 agcatcattt gaagctagaa aagtgaaagt tgaaaatata gctcaatcga tgaatgctca
    24961 gctaggaaca aaacgtgtct tggtagagct taatgaccaa tactacaata tgaaaaaagt
    25021 tatcgaaaaa gatatgactg ctattgagtt agctaaagaa gtaatggaag agcttgctat
    25081 caagcctgta atcgaaccca tacgtggagg aacagatggc tctaaaattt catttatggg
    25141 tattcccact cctaatattt ttgcaggggg tgagaatatg cacgggcgtt ttgagtttgt
    25201 tagcttacaa acgatggaga gagctgtcga tgtcattatt ggacttgttt gcaaagccta
    25261 attatcatgt aattgatatt tagtggaagg ggcttataaa aatgattaaa ctatttggta
    25321 aaataaggta tcactggcag ccagagttgt cttggtctat tatttattgg tctattgcgt
    25381 ttgcacctat ttttgtaggt ttatctttgt tgtatgagcg cactgaaata cctagtcggg
    25441 tttttattct atttgctatt tttgctgtcc tagttggcat tggattacat cgttatttca
    25501 ttattgaaaa taatggtatt ttaagaatcg tatcttttaa attgtttggt ccacgtaagc
    25561 tacttatttc tactattact aagattgagg tgaccaaatc aacactttgt ctgcactttg
    25621 aagataaaag ttatctcttt tatatgcgca aatggcctaa aaaatatttt cttgatgcct
    25681 tagcggttaa tccatacttc caaggagaag ttatattaag tgataacttt attaaattag
    25741 actattttga agtttatcag catgataaaa aagcccttac tcgagggtaa gagctttagt
    25801 tgggcaagat ttgacagcta aaatagcgtc cttatctgaa ggacaaattg actgacttgt
    25861 ttcagatgag cttgaaaatg tgacgatacc attgtcatga taatcaaaaa gtgatgaata
    25921 ggtttggcat agaccacaag cgatgcattt ttctggaatg atagatactt tcataactat
    25981 tattctaata tgattttgtt aaattaacaa ggggggacga tcatggctaa agaaccatgg
    26041 gaagaaaaaa ttgttgatga tactataggg acacgaacac gtaaatcaag aaatgctttc
    26101 attagcacgc cttggttgac tgctttatta agtgtattct ttgtcatcat tgttgctata
    26161 ctttttattt tcttttatac atcaaatagc ggtagtaata gacaagctga aacaaatggt
    26221 ttttatggag catccactca taaaaaaaca aggaaagctt ctaacgctaa aaaagcatca
    26281 agtagttcaa caactacaga cacaacacct tctagcgaag aaacacttgc ttctagtgaa
    26341 ggaactggcg aaacccttac tgtattggca ggtgaggggg cagcttctat tgcagcccgt
    26401 gcaggtattt ctgtggagca gttacaagca cttaatccag agcacatgac tcaaggatat
    26461 tggtatgcca atccaggaga tcaagtcact attaaataat tgaagaggaa aaaatgaaag
    26521 caattaaaat tgcgattgat gggccagctt caagtggaaa aagtacagta gccaagatta
    26581 ttgcaaaaaa tcttggctat acttatctag atactggtgc tatgtatcgc tcagcaactt
    26641 atatcgcact gacacatggc tatacgggta aagaagtagc ccttatttta gaggaacttg
    26701 agaaaaatcc tatttccttt aaaaaagcaa aagatggctc ccaattagtt tttcttgggg
    26761 atgaggacgt gaccttagcc attaggcaaa atgatgtgac taacaatgtt tcttgggtct
    26821 cagctttgcc agaaattaga gaagagctgg ttcatcaaca aagacgcatt gcacaagctg
    26881 gtggcattat catggatggt cgtgacattg gaacggttgt tctcccagat gcggaactaa
    26941 aaatcttttt agtggcttct gtcgaagagc gggcagagcg ccgctataaa gaaaacctag
    27001 aaaaaggaat tgaatcagat tttgagacgc taaaagaaga gattgcagct cgtgattaca
    27061 aggatagtca tcgtaaggtt tcacctctaa aagcagctga agatgccctt atttttgata
    27121 ccacaggtgt ttctattgat ggcgtggtgc aatttatcca agaaaaagca gaaaaaatag
    27181 ttgacatgtc ctagagccta tgttagaata ttaacaatga gaaaagtaga agtgagagct
    27241 tctcgccttg cgacttaggt tgtctggccc taacatgtca aattcctatg gaatattatg
    27301 tgtgcgggct tgatttatca agtccgcttt cgtttttctc taaaaaaata aagaggtgaa
    27361 gatcatagct aaaaaggatc tattcattaa tgatgaaatt cgcgttcgcg aagttcgtct
    27421 cgttggtctt gaaggtgaac aattaggtat taaaccattg tctgaagcgc aatcattggc
    27481 tgatgcttca aatgttgatt tggttttaat ccagccacaa gctgttcctc ctgttgccaa
    27541 acttatggac tatggaaagt tcaaatttga gtatcaaaag aaacagaaag aacaacgtaa
    27601 aaaacaaagc gttgtgactg tgaaagaagt tcgtctcagt ccagttatcg ataaaggtga
    27661 ctttgaaaca aaacttcgta acggtcgtaa attccttgaa aaaggaaata aagttaaggt
    27721 ttctattcgt ttcaaaggac gtatgattac tcataaagag attggtgcca aggtactagc
    27781 tgactttgct gaagcgactc aagatattgc tatcattgag caacgagcaa aaatggatgg
    27841 acgccaaatg tttatgcaac ttgcaccaat ttcagacaag aagtaattgt cacagtttca
    27901 ttatatcttt aagaggagaa tactaaaatg ccaaaacaaa aaacacaccg cgcatcagct
    27961 aaacgtttta aacgtacagg ttcaggcggt ttgaaacgct tccgcgcctt tacatcacac
    28021 cgtttccacg gaaaaactaa aaaacaacgt cgtcatcttc gtaaagctgg tttggttagc
    28081 tcaggagatt tcaaacgtat caaagcaatg gttactggtc tttaatttta aaggcagtag
    28141 acatttacga gtattataag aattatttga aggagaacta ttaatggcac gtgttaaagg
    28201 tggcgttgtt tcacgcaaac gtcgtaaacg tattttaaaa ttagctaaag gttactatgg
    28261 agcaaaacac atcttgttcc gtactgcaaa agagcaagtg atgaactctt actactatgc
    28321 ataccgtgat cgccgtcaga aaaaacgtga tttccgtaaa ctatggatca ctcgtatcaa
    28381 tgcggctgct cgtatgaacg gtttgtcata ctcacaattg atgcacggtt tgaaacttgc
    28441 tgaaatcgaa gtaaaccgta aaatgcttgc tgatttagca gtagctgatg cagcagcttt
    28501 cacagctctt gctgatgcag ccaaagcaaa acttggcaaa taagaaacgc tatacctaaa
    28561 aaataacttt ctattaccta tagaaagtta taaagaaaca aaaatgaagg agacgatgga
    28621 cgtcttcttt ttattatact caatataata aaaagcagtt tcccatgttt tattaccagt
    28681 aatgtgggat atttagatgg tagcaaggag ttttatagct gatttgtttt ctttaggtct
    28741 aattagcata ttttgattac tgataaactt gaatatcgcc aataaaaaag aagcaaaatt
    28801 attattttgc ttctagtcag atcattaaat tgattattct ttatttttgg ggttatcctc
    28861 ttttagttca gaagttgccg cgtcagcttc tacgggattt agttctaagt aagatggtgc
    28921 tttaaaaagt ttctgagttg atttatgacc atttttactg tataaacttg ttgactctga
    28981 tccgagtttt gctgaaatat ctttcagttg ttttaactgc ttagtgtaga taaactggtt
    29041 aggatcaata gcttttagac cattttgagt atcaaagcgc aggagatcac ccgtttgaat
    29101 agcgtcgcta gctgctagtt gttgagcaac agcagagcga atttccttag ttttggctat
    29161 agtcatttca tcgggattag tgatttctaa acctgtttgc gtgttgtaca gacgtccact
    29221 atagttagtg tattcaggag tcatataagt cccagatgtt cgttgtgcca caatctggct
    29281 attttgaggt gataataagt cttgtcctaa ttgaacaaac tgactagtgt caataccaag
    29341 tatgtggagt aaagtaggaa gagcatcgat ttcaccgcca aaggtttctt tgatactacc
    29401 attcgtataa cctggaatat ggatcatata cgggactcgt tgtagcatag cattatcata
    29461 ttcagaccaa gtttcagaat ctttaccaag aagtggagca agactagaat tacgagaatt
    29521 tgaaatacca taatgatcac catataaaac aaaaatagaa ttctcgtaca aaccagtggc
    29581 tttcaagtaa tcaaaaaaag atttaagggc agcgtctaaa taattggcag tagcaaagta
    29641 accattgatc gtttcatcgt ctgttttggc taaaggaaaa ccttcttcac tactttcgcc
    29701 ttttagacta gtataaggat agtgattact cacagtgata aacttggtat aaaaaggttg
    29761 ttgcatctgt tcaagatatt ttatggaatc cttaaacatg tacttatcat ttaacccata
    29821 ttgaaaagag ttcttactat tttgtttaga gaagtagcta gaatcaaaaa aataattata
    29881 gccccattgt ttataagcat tattgcgatt ccagaaagtt ccaacgttac catgaaagac
    29941 agcgctggta tagccacctt tttgggttaa aatacttgga gtagcaaatt gtgtattttc
    30001 accaccgtag ttcaccataa aagaaccact atttaaacca aataaggaat tctccatcat
    30061 ggtttcagca tcagaagttt tacctgcttt aacctgatga aaaaagttag ggaatgctaa
    30121 ggtagcattt gagtgataaa gcgagttgat aaaaggtgtt acctcatatt ctttatctcc
    30181 ttcttttagt ttataatcta ttaagaattg ttgaaagctt tctaaatgaa gaacaataat
    30241 atttttgcct tttccaattc caaaatactg aggatcaggt gctgcataat gtcctttgac
    30301 ataagtttta acatcaatta attcttctgc agtagctcca tttcgttctt tttgagcttg
    30361 ataggtttga ttgccgctat agagggtaaa cgctggtagt cctaaagctc tcacaatata
    30421 agtatttgaa aagccacgtg ttaatagttc cggacgatca atttctgcta aaaaaagatt
    30481 aatagaaagc aatagactag ataaagcggt gatggcaaat gctgcgcgct tgttaaaagg
    30541 acgttggtca tttttgattt ttttagtgat agataaacta attagaataa taaaatcaag
    30601 aatatagata atatcccaga tgcgtaaaag gtttaaagca gagtccccta atccagcgga
    30661 gactttacta ctagctagca tggcactcac tgtaataaag tctgaaaatt ctcggtaata
    30721 tatagaattt gatatcaaga gaatgttaag gataatataa accacccaag aacagatata
    30781 aaaggctcgg gtattcttaa cgtaaagagc aacccccaaa agtaaaaaag ctagtgggat
    30841 tggattgata atagttagaa agacttggta tatatttccc aaatctagtg aaaagtctgt
    30901 atgatatgcc cacagggtct ttatccagta acaaaagagt agagtaatta taaaacccaa
    30961 tcttgtgttg ataaaacctg tgataagtgt tttaaatttc ttcacagtag caacttcctt
    31021 atcgtttttt cctaaagatg tcatctccaa ataaaatctc agattaaatt ataccaaaaa
    31081 tgtgaagcta atgcttgttg taagttcaaa tttagtagga ttttttatca gattttgtta
    31141 taataaaaac tatgaataaa ctctatattg attcttttgt cgaaaagaag ctgacagcag
    31201 gggtacaatt attagatgaa aaagattttt ctaatataaa ggaaaaaaat cagttggttc
    31261 aactggtgac caagtccaat cgtcccattg gaacagcata tatctctaag caaaataaag
    31321 gaattggttg gtatctagga ccagaaaaaa tcgacttgtc tatctcttat tttgttagtc
    31381 tattttcagt tgctaaggcc aaaagacaag attttgctca atcggacgag accaatgctt
    31441 atcgcctttt taatcaagaa ggagacggtt ttggtggagt aacaattgat ctttataaag
    31501 attttgcagt cttttcatgg tacaatgcct ttgtctatga caaaaaagaa atgattatgg
    31561 aggcttttca acaggttttt cctgaagtca aaggagctta tgaaaagtgt cgctttaaag
    31621 gtccagatac tgaaactgct catctgtatg gtgagctggc acaagaaacc ttttcgattt
    31681 tagaaaatgg catagcctat caagtcttct tgaatgaggg cttaatgaca ggtgtttttc
    31741 tagaccagca tgatgttaga agagcactag tagatggttt agctatgggc aagtcccttc
    31801 ttaacctatt ttcctatacg gctgcctttt ctgtagcagc tgctatgggt ggtgctatcg
    31861 agaccacttc tgtggacttg gctaaacgat cgagagaatt atcactagct cattttgagc
    31921 ataaccagtt aaacttagcc tcacatcatt ttgtggtgat ggatgttttt gaatacttca
    31981 aatatgccaa acggaaaaaa ttaatctttg atgtgattgt gattgaccca ccaagctttg
    32041 ctagaaataa aaaacaaact ttctctgtga gcagggacta ccacaaatta atcacagagg
    32101 ccttggacat attgagtcct aagggaacta ttatagcttc aacaaatgct gctaatatga
    32161 ctgttagcca gttcaaaaag caaattatca aaggatttgg aagtcgtcgt cctgagtcta
    32221 tgactctcca gcagcttccg agtgacttta ctatcaataa agcagatgaa agaagtaact
    32281 atttaaaggt atttactata aaggtacgag aatgagaatt gtagcaccag tcatgccaag
    32341 acattttgac gaggctcaag ccattgatat ttctaaatat gaagatgtta atttaattga
    32401 atggcgagca gactttcttc ctaaggatga gattgtcgca gtagcaccag ctatttttga
    32461 aaaatttgcc ggaaaagaaa ttatctttac ccttcggacc gttcaagaag gggggaatat
    32521 taccctttca agtcaggagt atgttgatat tatcaaagaa atcaatgcta tttataatcc
    32581 agactatatt gactttgagt attttacgca caagtcagta tttcaagaaa tgcttgattt
    32641 tccaaatctt attttgtctt atcacaactt tgaagagact cctgaaaatt taatggaagc
    32701 tttttcagaa atgaccaagt tggcaccgcg tgtggttaaa attgctgtga tgccccagag
    32761 tgaacaagat gttctagatt tgatgaacta taccagaggc tttaagacgc tcaatcctga
    32821 gcaagaattt gcaactatat ctatggggaa attaggaaga ttgtctcgtt ttgcgggaga
    32881 tgtcattggc tcttcctgga catacgtttc attagatcat gttagcggtc caggtcaagt
    32941 gactctcaat gatatgaaac gtattattga ggtcttagaa atggatatct ctaactagtg
    33001 aatggtctaa aaatattatt tctacaatag ttaagaaatg atgtttttag attaatgttt
    33061 ttaaaataag tggaaccaaa aaggagaaaa attgcgttat ttaacagcag gagaatctca
    33121 tgggccatcc ttaacagcta ttattgaagg tataccagca ggattaactc ttcatccagc
    33181 ggatattgat cacgaattac agcgacgtca aggaggttat ggccgtggtg ccagaatgtc
    33241 tattgagact gatcgggttc aaatatcctc aggagttcgt catggcaaaa caacaggggc
    33301 ccccattacc ttaacggtaa ttaataagga ccaccaaaaa tggctagatg tcatggctgt
    33361 aggagacatt gaagagactc tcaagttaaa gcgtcgtgtt aagcatccta gaccaggaca
    33421 tgcggatttg gtaggaggta ttaaatatca ttttaacgat ttaagagatg ctttagagcg
    33481 ttcctcggct agagaaacaa cgatgagagt ggctgtaggc gctgttgcta aacgtatctt
    33541 agctgaactt ggaattgata tgttacatca tatcttaata tttggtggta taacgatcac
    33601 aataccttct aagctctctt ttagagagtt gcaagaaagg gctctccatt cagagttatc
    33661 tattgttaat ccaaagcaag aagaagaaat taaaacgtat attgataaga taaaaaaaga
    33721 aggggatact attggtggta ttatcgaaac gatagttcaa ggggtgccag caggtcttgg
    33781 atcttatgtc caatgggata aaaaattaga tgccaagctg gctcaagcag ttctttccat
    33841 taatgctttt aaaggggtgg aatttggagt tgggtttgat atgggattcc aaaagggctc
    33901 tcaggtaatg gatgaaatca cctggacacc tacccaaggc tatggtcgtc aaacaaacca
    33961 cttaggtggc tttgaaggtg gcatgaccac aggacaacct cttgttgtta aaggggttat
    34021 gaagcccatt cctactttgt ataaaccact catgtcggta gatattgata gtcatgaacc
    34081 ttataaagca acggtggaac gctctgatcc gacggcctta ccagctgcag gggttatcat
    34141 ggaaaatgtg gtggcgacgg tgcttgccaa agagatttta gagacttttt cttctactac
    34201 catgtctgaa ttgcaaaaag ctttttcgga ctatcgtgcc tacgtcaagc aattttagat
    34261 agcgagatac tggcttttat ggtatcatac aggtattaat gaacaaaagg agactgtcat
    34321 gtcacaagaa atttatgact atgctaatca acttgaaaga gcagttcgtg ctctgccaga
    34381 ataccaaaaa gtgttagaag taaaagaggc cattcaagca gacgctagtg ctagcgaact
    34441 gtttgatgag tttgtcgcta tgcaagaaaa aatccaaggg atgatgcaat caggccaaat
    34501 gccaacggct gaagaacaaa caagcattca ggaacttagc caaaagattg aagctaatga
    34561 ccagctaaaa gcttattttg aagcgcagca ggccttatct gtttacatga gtgatattga
    34621 gcgtattgta tttgctccgt tgaaagattt agtcaagtaa gccaattaag ataggaaaaa
    34681 aaacagattg ctggcagctc gtcaggatct gttttttagt gtttattttc tttgtatcag
    34741 aaagttgagc ctctttctta tttgtgatat agtcaaataa attgaccaaa atagcaggcc
    34801 agtagtggct ttaatgaagg caagttgtct gctaactggc tatgttaaaa catcagtatt
    34861 agaaagtaaa aggttataaa aatggttatt ccttatgatt atattgttat tggtggaggg
    34921 agtgcaggta ttgcttctgc gaacagggca gccatgcatg gggccaaagt attgttagct
    34981 gaaggtaaag agattggggg aacctgtgtt aacctaggct gcgttcctaa aaaagtcatg
    35041 tggtatggcg ctcaagtagc agatatttta gggacttacg ctaaggatta tggatttgac
    35101 tttaaagaca aagcatttga ttttaaacaa ttgaaagcaa atcgtcaagc ttatattgat
    35161 cggattcatg cttcttatga gcgaggcttt gagcagaatg gtgttgaccg tatttatgat
    35221 tatgctgttt tcaaagatgc tcatacggta gaaattgcag ggcagcttta taccgcaccg
    35281 catattttga ttgccacagg aggacatcct gtttttccag atattgaggg ggcacaatac
    35341 ggtatcagct cggacggctt ttttgcctta gatgaggtac caaaacgtac ggctgtggtt
    35401 ggggcaggct atattgctgt ggaacttgcc ggtgttttgc atgctttggg gtcgaagacg
    35461 gatttgttta tccgtcacga tcgaccttta agaagttttg acaaagccat tgtggatgtt
    35521 ttagttgagg aaatggcagt tggtggtcct tgcttacacc ctcattcaga agtggcaaag
    35581 gttgtcaaaa atactgatga gtccttgacc ctttacctca aagatggcca agaagttgag
    35641 gttgaccagc tcatttgggc tatcggtcgc aagcctaatc tagaaggttt tggtcttgac
    35701 aaaactggag tgacgttaaa tgataaaggt tatattgaga cagatgccta tgaaaacacg
    35761 tctgtcaaag gtatttatgc cgtgggagat gttaatggga aactggcttt aactccagtt
    35821 gctgtggcag ctggacgtcg cctatcagag cgtcttttca atggcaaaac agacgagaaa
    35881 ttggactacc aaaacgttgc tactgtgatt tttagtcacc ctgttattgg ctctgttggc
    35941 ttgtcagaag aagcagcagt caaacagtat gggcaagaag cagttaaaac atatcaatca
    36001 cgttttacgt ctatgtttac tgctatcact aaccatcgcc aaccttgtct gatgaagttg
    36061 gtaacagtcg gagataccga aaaaatagtt ggtcttcacg gaatcggcta tggggttgat
    36121 gaaatgattc agggctttgc agtagccatc aaaatgggag caaccaaggc ggattttgat
    36181 aacaccgttg ctattcaccc aactggctca gaagaatttg tcaccatgcg ctaaaaaatg
    36241 tagcctaagg caataaaaaa tgatgttaac agtttttgag gaaagtcttc taaactgcta
    36301 gcatcgtttt ttgttaggtg attaggggaa tatgttttta aagatatttg cgttgctcag
    36361 acaatattgc cttacctcgg agataaaata aagtgaccct gtaacaacaa agagttcatc
    36421 agatgttacg gaaccttgta accagtctct gtaatctttt acctgtgggt atttatcagg
    36481 ataatcctct aaaagatttg cttcagggaa atcaaaagtc gtgacagata cttcaaaagt
    36541 atctagttga gccagcaagt cggctaaagg cttacgtttg agacctgcaa agagaatatg
    36601 aataggacgt ttgggaaatt cttcttgcag tagagacttt aatttagcaa tggaatctgg
    36661 attgtgagcg ccgtctaata gaagattagg ttgaataaat tcggaccggc caggccaagt
    36721 tgttgcctca attccttttt gaatcgtttt tggagatatt ttaggaaagg attttctaag
    36781 taattgagct gtcataatgg ctaaacaggc attcgctttt tgatgttgac ctaatagttt
    36841 gagacggagt ttggttaaat tcaagttatc gtgttgaaag gaaaacgtct tttctgaagg
    36901 attgagcaaa aaatcttttc ctaattgata taagcgtgaa ccggcttttt gggtaacttg
    36961 ttgaaagact tgactagcct cctgctcaag ttgaccgata ataacgggtt ctttagcttt
    37021 gataacttct gctttttggt gagcaatttc tgctaggcta taacctaaac gttcttggtg
    37081 atcaaaacta atagaagggc atacgacggc cagagcatga aaaacattgg ttgcgtcggt
    37141 tttcccacca atccctgcct caataatggc aatatcaaca ggtttaagat tggcaaagta
    37201 gcaaaacatt atgagagtca ctaactcaaa ctcggtaggt ctatcccaat ttgtttcaag
    37261 ggacatttta gtgagaatgg gtctaatcac gctcacacat tgcgccaatt ctaggtctga
    37321 aatgggctgg ccattaaggc aaatccgatc gtgaaaagag ataataaaag gagatgtaaa
    37381 ggttccgacc tgataaccag aagttgacag gatatgctgt aagtaggctg tcacagagcc
    37441 cttgccgtta gttccaacaa cgtgaatagc agggaatttg tcttgtggcc ggcctaatgc
    37501 ctcaagtaac caagccattc tggccaaatc ggtcttgcga ccattagcct taaaggtatg
    37561 aatccaggaa atgctatcat aggtagtcat tactatttct tgtccttcta acaatgcttt
    37621 taagagttcg tagggagacc agtattagca tcctcagctc cagttgcatt aggaagttgc
    37681 gtcccttcag tggctgatgg tgtctcagga gctgtaggag ttgtctcgtt gtgagaaggt
    37741 gtggtagaag atgacggaag agttgtctta ttatcatctg tggtttctga agtacctgta
    37801 tggttttgat taccctgaga agactctttt tcaataatct tgacaatggt attactcgat
    37861 tctttgggtg tatcattaga ggtcttatct ggtacaggct gcagtgctct tgtaatggta
    37921 atggttgcca caatgatact caaaatacta ccaataagtg caatggattt ttggaaagct
    37981 gtaaaacctg ttttaatatg ctcggtaggg acacctcctg atttacgagg tttttgttga
    38041 cgggaataac gtgatgacat atttttctcc tttaaaatca tcgtaatgcc aagattaagg
    38101 aggaggaaat agaagtccta gctcctcttt aacacttcat ctatcagtat aatctagaaa
    38161 aaaagtgaag tcaacggttt accttatgtc taggacaagg aagtcctttg ggcttattgt
    38221 aatacgaata ttcttgattt cattttagct attttgtgat agaataaagt attgagtaaa
    38281 aatgaaggtg acacatgatt tattttgata atgcagccac aaccatacct tatggagagg
    38341 ctcttaaaac ttaccaagaa gtagcaacaa aaatatatgg caatccctca agtctgcacc
    38401 aattagggac aaatgcgtct cgcattttag aagcttcccg taaacagatt gctggtttgt
    38461 tgggtgttaa gtcagaagaa atctttttta cgtctggtgg gactgaaagt gctaattggg
    38521 ctattaaggg aattgctttt gaaaaaactg cttttggcaa acatattatt atctcagcca
    38581 ttgaacaccc tgccgtgagt gaaagtgtca aatggcttct cacacaagga ttcgaagttt
    38641 cttatgctcc agtgacaaca cagggagttg ttgatgtgaa tgctttagct gagctgatta
    38701 gaccagacac catcttaatc tcgattatgg ctgttaataa tgaaatggga gctatccaac
    38761 cgattagagc catttcaaac ctattggcta accaaccaac gatcaccttt cacgttgatg
    38821 ctgttcaagc cattggtaaa ataccactct gtgattatat gactaatcgt gtggatttgg
    38881 cttctttttc agggcacaaa tttcatagtg ttcgtggtgt tggcttcctt tataaaaaag
    38941 caggcaagcg actcaatcct ctcttgtcag gcggcggcca agagcaggag ttacgttcaa
    39001 ccactgaaaa tgttgcagga attgctagca tggccaaagc attaagaata gtcactgaaa
    39061 aacaagtgag cgtcttacct aaactaacag cgatgcgaga tgtgatttac aaagctttgt
    39121 ccgcttaccc agatgtgaca gtattttcag ctcaagaagg ctttgcgccc aatattttga
    39181 cctttgggat tagaggggtt cgaggagaag ttattgtcca cgcctttgaa aaatatgaga
    39241 tttatatttc gaccacaagt gcatgttcgt ccaaagcagg taagccagca ggaagcctag
    39301 tggctatggg gattcctgta aaaactgccc agacagccgt tcgtattagt ctagacgatg
    39361 acaacgatat gggacaagta gagcagtttt tgaccatatt caaacagatt tatgataaaa
    39421 cacagaaagt taggtaagat taggtaagat ggactattca gaaattatgg ttcgccatgg
    39481 tgaactgtca actaaaggga agaatcgcat gcgattcatt aacaaactta aaaacaatat
    39541 tcaagacgta ctggctcctt tcccagccat tgctgtccgt tcagatcgtg ataggaccca
    39601 tgtgtctcta aatggcacag attatcagcc tattgtagaa gctttaaagc tggtgtttgg
    39661 tgttcaagct ctatctcctg tttataaact tgaaaaaagt gtccctcttt tggtaacagc
    39721 tgttcaagat atcatgactt ctctttatcg tgatggactg acgtttaaga ttgccactaa
    39781 gcgtagtgat cacgcgtttg aattggatag tcgtgagctt aacagtcttc ttggtggagc
    39841 tgtttttgag gttttaccaa atatccaagc ccagatgaaa catcccgatg tgactctcaa
    39901 agtcgaaatt cgtgatgaag cagcttacat ttcttatgaa gagattaagg gagcaggcgg
    39961 gttaccagta ggaacgtcag ggaaagggat gctgatgtta tcgggcggta ttgattcacc
    40021 agtagctggg tatttagccc ttaaacgtgg cctcgatatt gaagttgttc attttgctag
    40081 ccctccgtac accagtccag gtgccttggc taaagcccaa gaccttactc gtcggttaac
    40141 tcgttttggt ggaaatatcc aatttatcga ggttcctttt acagaaattc aagaagaaat
    40201 taaaaataag gcaccagagg cttaccttat gacattaacc cgccgcttta tgatgcgcat
    40261 cacagatgcc attcgtgagc aacgtaaggg tcttgttatt gttaacggtg agagcctagg
    40321 tcaggttgct agtcaaaccc ttgaaagcat gcaggccatt aatgcggtaa catcaacccc
    40381 aattattaga ccagtcgtga caatggataa acttgaaatt atcgagatgg ctcaggcgat
    40441 tgatactttt gacatttcta ttcaaccgtt cgaagattgc tgtactattt ttgcaccaga
    40501 ccgtcctaaa accaacccta aactaggtaa tgctgaaaaa tatgaagaac gctttgatat
    40561 tgatggtctt gtccagcgtg ctgtgtcagg aattgttgtc acagaaatca cacctgaaat
    40621 cgtcaatgat gaggttgaaa atctcattga tgctttactt taaaagaaaa gatcaagtgt
    40681 aaatctttta gtagtcagta atgatactat tgaaaagcct ctataagggc ttttttgctg
    40741 caatgaagga ggaaaatttt gaggcaagat gttataatag agaaaaagga gggtgtgtct
    40801 tgattaaaac tatgcaatac aaaaaaacga tggcaacagt tgttgctctt atcgtcgcac
    40861 ttttgctatt tggcttgatt tatgatttac ttggggttca aaaaaataag ctagcagctc
    40921 aaaaatctgc tcagccaaag gtcaaaacag cgcgtgttgt agctaatggc gacattttaa
    40981 ttcatgatat tctctacatg agtgctagaa aagctgacga tacctatgat ttcactcctt
    41041 attttgaata tgttaaggac tggattagcg gtgctgactt agctattgga gattatgaag
    41101 ggaccattag cccagattat cccttagcag gctatccctt attcaatgct ccagaagaga
    41161 ttgcaggtgc tctgaaaaat actggctatg atgtagtcga tttagcccat aatcatatct
    41221 tagattccca attagacggt gcccttaaca ctaaaaaggt ttttcatcaa ttaggtatag
    41281 acagtattgg catctatgac aaggatcgtt caaaagaatc cttcttaatt aaaaatgtca
    41341 atggtatcaa aattgcaatt ttaggttatt cttatggtta taacggcatg gaggccacac
    41401 ttagccaaga agactatgag aaacacatgt ctgatttaga tgaagctaag ataaaaaaag
    41461 aacttcagct agctgaaaaa aaggctgacg tgaccattgt catgccacaa atgggaacag
    41521 aatatgcctt agaaccgaca gcagagcaaa aagaacttta ccacaaaatg attgattggg
    41581 gagctgacgt cgtcctagga ggccatccgc atgttattga accttcagag acagttataa
    41641 aaggtaggca aaagaaattc attatttact ctatgggaaa ttttatttca aatcagcggc
    41701 ttgaaaccgt agatgatatc tggactgagc gtggcctatt gatggacctt acttttgaga
    41761 aaaaagataa caaaacaaaa attaaaacag ttgaggccca tccaaccatg gttttagcca
    41821 aaggtaaagg cattgtgggt aaagaaggtt ttgaactata caattatcga acaatggttt
    41881 tagaagattt tatcaaaggt ggaaaatacc acgacaagat tgatgaggaa accaaagcaa
    41941 aagtagcact tgcttatcag gaaattaatg atttagttaa cctgaaatgg taatgtttgg
    42001 tggtttagca tctaacgata aaaaatagag attaaactta atttttcctt gataaaaagc
    42061 agttatggtg ctaaaatagt aacgttgact aaatgcacat cctgtgcaac cgcgcgaaaa
    42121 tcgttgtcaa gtagaccaag tctcacgatt ccaggcgagt cttcacagag ggaaacctca
    42181 aacaaaaatt ttataggagg tgcatcatga gcacatacgc aatcatcaaa actggtggaa
    42241 aacaagttaa agttgaagta ggtcaagcaa tctacgttga gaaaattgac gctgaagctg
    42301 gcgcagaagt cacttttaac gaagttgttc ttgtaggtgg tgacaaaact gtagttggta
    42361 ctccagttgt tgaaggagct actgtcgttg gaactgttga aaaacaagga aaacaaaaga
    42421 aagttgtaac attcaaatac aaacctaaaa aaggtagcca ccgtaaacaa ggtcatcgtc
    42481 aaccttacac taaagttgtc atcaacgcaa tcaacgctta atttactagc ttatgattaa
    42541 agcaattttt actcgccaaa aaaatggtca actgagcagt gttacactga caggtcatgc
    42601 tggtagtggt aaacatggct ttgatattgt ttgtgcctct gttagtactc tagccattaa
    42661 ctttgttaat tctcttgagg tgttagcaga ttgccaagca ttagtagatc ttaatgatgt
    42721 tgaaggtggg tatatggcta ttacaatacc accacatgac aacaaggagg aagttcaatt
    42781 attatttgaa tcttttcttc taggaatgac tagtttggcg aaggattctt cgaaatttgt
    42841 caacactcag gtcatctaaa tttaatttaa gagaggaaac aacattatgt taaaaatgaa
    42901 tcttgctaac ttgcaacttt tcgcccacaa aaaaggtgga ggttctacat caaacggacg
    42961 tgactcacaa gctaaacgcc ttggtgctaa agctgctgac ggtcaaactg tttcaggtgg
    43021 atcaatcctt taccgtcaac gtggaactca tatctaccca ggtgtaaacg taggccgtgg
    43081 tggagatgac acactttttg ctaaagttga aggtgttgta cgtttcgaac gtaaaggacg
    43141 tgataaaaaa caagtttctg tttacccagt agctaaataa ttgtttaaca tcgcattata
    43201 taaggctttc caggtgtctg gaaagccgat ttttttattt tggtactcat gttgctatcc
    43261 atattttaaa aatcaatgta ggaagagagc ttgtcagcag ttttatcttt tgaggattta
    43321 gtaacatgag cgtagatgtt catggtagtt tggatagttt catgaccaag tgtattggat
    43381 tttcattgtt tgtttctatc ctactaaaga ttgtagttgc ttatttagag caatagttct
    43441 aattgtaaaa agtcagcata ataaaacaat gtcataagat gttgttataa cgggctttat
    43501 ggtataatta aggatactca ttttaggaag gaaagcaatg aatattcaac aattacgcta
    43561 tgtggtagcc atcgctaata atggaacttt tcgagaagct gcctctaaat tgtttgtcag
    43621 tcagcctagc ttatcagttt ctatcaaaga tttagaggcg gaattagggt ttcaaatttt
    43681 taatcggact acttcaggaa cagtcttgac aagtcaagga ctagtttttt atgagaaagc
    43741 tttagaagta gtcaaatctt ttgattcttt tgaaaaaacc ttttctcaag ctgatctgga
    43801 tcaaaatgaa ttttctattg ctagccaaca ttatgacttt ttgccacctc ttattacagc
    43861 tttttcgcaa caatacgatg gtcatcgtgt ctttcgtatc tttgaatcaa caactattca
    43921 aattttagat gaggttgctc aaggaaacag tgaaattggt attatttact tgaatgtaga
    43981 caatcaaaaa ggtctctttc agcggatgga taagctgggc ttggaatatg tttctctaat
    44041 tcctttcact actcatattt atctttcaaa aacgcatcca ttggctaatc gcgaagcgct
    44101 ttatttgagc gatattcaag gattacctgc ggtaaggttt acccaagaaa gggacgagta
    44161 tttgtattat tctgaaaatt ttgtggatac tagtgaatgc cctcgtattt ataatgtgtc
    44221 agatagggca accttgaacg gtattttaga gagaaccaat gcctttgcga caggatcagg
    44281 ttttctcgat catcggagtg ttaatgggat taaagtgata cctctagctg atcatattga
    44341 caatcaaatg atttatgtca aacgaaaaga taaaaacctt tctgttgctg gggccacttt
    44401 tgtgactatt ttaaaagact attttgataa aagaaggaag gccttaacat gaaaaaacga
    44461 ttgtttgtgc ttagcttgat cctccttgta gctttggatc aacttagtaa attttggatt
    44521 gtttctcata tagcgcttgg agaagtgaaa ccctttatcc caggtatcgt cagcttgact
    44581 tacttgcaaa acaatggggc cgccttttcc atattgcagg accagcaatg gttctttgtt
    44641 gtcataacgg ttttagttat cggttatgct atttattacc ttgctactca tccccattta
    44701 aatatctgga aacaattagc tctcttgctt attatttctg gtggaatcgg gaattttatt
    44761 gatcgtttgc gtttagctta cgtgattgat atggttcatt tagactttgt ggattttgcc
    44821 atttttaatg tggcagattc ataccttacc gttggtgtca tattattagt gatatgttta
    44881 tggaaagaag aggattatgg aaattaatgt gataacatcg gggcaacgct tggataaggc
    44941 actagctgat ttaagtcccc tgtcacgtgg tcaagccaat gaccaaatta agcaaggcct
    45001 tgttttagtt aatggtcagc aaaaaaaggc taaatatacg gttcaagcag gagatgtgat
    45061 ttgttttgaa ctccctaaag aagaagtctt agagtaccaa gctcagaata ttcccttgga
    45121 cattatctat gaagatgata ctctcgccat tattaacaag ccacagggaa tggtagttca
    45181 cccttcggcg ggacaccctt caggaacaat ggttaatgcc ttgatgtatc atatcaagga
    45241 tttgtcctca atcaatggtg tggttcgtcc aggaattgtt catcggattg ataaggacac
    45301 ctcaggtctc ttaatggtag ctaaaacaga tgcagctcat caagccttgg cagaagaatt
    45361 aaaggctaaa aaatctctca gaaaatacct cgccattgtc catggaaacc taccgaatga
    45421 tcgcgggatg attgaagctc ctataggtcg tagcgaaaag gaccgtaaaa aacaggctgt
    45481 tacagcaaaa ggcaaagaag cagtgacacg ttttacagtg ctagaacgtt ttggcgatta
    45541 cagtctagtg gaattacaac tggaaactgg tcgaacgcac caaattcgtg ttcatatggc
    45601 ctatattggt catcctgttg ctggggaccc tttatatgga ccacgtaaga cactatcagg
    45661 tcatggccaa tttcttcatg caaaaacatt gggattaacg catcctatga caggaaaaga
    45721 aatgatattt actgttgaag ctcccgaaat ttttcaaaag gtgttaaagc tgctaagaaa
    45781 aaaataaaca gcgaggagta gccattattt ttagtcttat atctttgtgt aaagttttta
    45841 acattataaa aaatactaaa aagcatttac gataagccag gaaatatgag aagtctttta
    45901 tgttattggg atatgcaaca tatctgttag agatctttat ttaaactctt aaagaaaaga
    45961 gatattccaa atagatgcta ggtattgtct tttttacttg tttttctttt ctttttttgc
    46021 tatactgatt gaaataaaat attcctttaa aacagtcctg tgaggctgtc aaggagacgg
    46081 attaaaaaca tgcactatga tacttaattg tgctatattt tgctgaatct ccttgtttcc
    46141 aaggagatct ttttatttga atttttagag gaggtttcaa catgaaaact aaggaaattg
    46201 ttgatgacgt gaccatgaaa cgcgccatta cccgtattac ttatgaaatt attgaacgta
    46261 ataaacaatt agacaatgtg gttttggcag gcatcaaaac gagaggtgtt tttctagctc
    46321 gtcgcattca agaaagactg catcaattag aagggttgga tttaccaatt ggtgagttag
    46381 acatcaagcc ttttagagat gacatgagag ttgaggaaga cacaactctg atgtcagtag
    46441 atattacagg caaggatgtg attttgattg atgatgtgct ttatactggt cgtaccatcc
    46501 gcgcggccat cgataactta gtgagtttag gtcgaccagc ccgtgttagt ctagctgttt
    46561 tggtggatcg tggccatcgg gaattaccga ttcgcgcgga ttacgttggg aaaaatattc
    46621 caacaagcag tgtagaagaa attgttgttg aggttgtcga agttgatggc cgtgatcgtg
    46681 ttagcattat tgacccaaca taagaaggag gttttactgt gaaagatgtc atttatgatg
    46741 tggaagaagt gccaaaggca ggtatgttag ttggtttgtc ctttcagcat ttatttgcca
    46801 tgtttggggc gacggttctt gttcctattt tagttggaat tgacccgtct gtggccctcc
    46861 tatcgagtgg tttaggaaca cttgcacatt tatcggtaac caaatttaaa attccagcct
    46921 acatgggctc aagttttgct tatatagcag ctatgcagtt actgatgaag acaaatggta
    46981 ttggtgctgt agctcaaggg gctatgacag gaggtctcgt ttacctgatt gtagctttga
    47041 ttgttaaagt tattggcaat gattggattg ataagatttt accaccgatt gtagttggtc
    47101 cgattgttat ggtcattggt ctaagtctag cttctacagc tgttaatgat gtcatgttaa
    47161 aaaatgggaa ctataatctg acttatcttg ttattgggtt agtcacctta ttatcagtta
    47221 tttttttcaa tatttatggt aagggaattg ttgctattgt accgctttta ttagggctat
    47281 tagttggtta tgttgtagcc cttttagtgg gtgttctcac cggtcaagaa attgttgatt
    47341 ttaccaatgt ggctcaggcc aaatggttta gtatcccatc agtggaaatt cctttcttaa
    47401 cctacggggt taaattctac ccaagtgcca ttttaactat ggcacccatt gcctttgtta
    47461 caatgacaga acattttgga cacattatgg tcttaaatag tttgacaaaa agagattatt
    47521 tcaaggatcc aggacttgaa aagactttaa ctggtgatgg ttttgcgcaa atcattgctg
    47581 gcttcttagg ggcacctccg gtcacttctt atggtgaaaa catcggtgta atggctctga
    47641 ataaaatatt ctcggtctat gttattgcag gtgccgcagt gattgccgct ctcctcagtt
    47701 ttatcggtaa ggtatcagcc ttaattcaat ctattccaac accagttatt ggaggtatat
    47761 cagttgcctt gtttggtgta atagcttcta gcggtttgaa aatcttgatt gaatccaaag
    47821 ttgatatgga taataagaaa aacttattga ttgccagtgt cattttggtc tctgggattg
    47881 gaggattgat gcttcaagta aatggtcttc aaatttcagg tgtcgctttc tcaacacttc
    47941 ttggcatcat cttatatcag gtacttcctg aaaaatagga agaattgttg ttagaccact
    48001 tacatagtca tcagtcgtta gtgataaagg ggataatcat gtcagttgta aataatcgcg
    48061 ttgcacttac caatttagtt tcaatggaag cattaaccac cgaagaagtt ttaggtctta
    48121 tcaaccgtgg ttcagaatat aaggctggta aggttgttat ttcggatcat caaaaggatc
    48181 ttgttgccaa tttattcttt gaaaactcca cccgaaccca taagtctttt gaagtggctg
    48241 agaagaaact cggcttgaca gttcttgatt tcaatgctga tgcaagtgcg gttaacaagg
    48301 gagaaagttt gtatgatact gtgttaacca tgagtgcact tggaacagac atctgtgtga
    48361 ttcgtcaccc agaagatgat tattacaagg aattagtaga aagtccaact attacagctt
    48421 ccattgtcaa tggaggagat ggttctggac aacacccgag tcaatgttta cttgacttat
    48481 taaccatcta tgaagaattt ggacgctttg aaggcttaaa gattgcgatc gctggtgact
    48541 taactcactc tagggtggca aagtccaaca tgcaaatcct caaaagatta ggagcagagc
    48601 tttatttcta tggacctgaa gaatggtatt cagaagcctt taatgcttac ggcacttata
    48661 ttgctattga ccagattatt aaggaattgg atgttcttat gttgctgcgt gtacagcacg
    48721 aacgtcatga cggccatcag agtttttcaa aagaaggtta ccatcaagcg tttggattga
    48781 cacaggaacg ttaccaacaa ttaaaagatt ctgctattat tatgcatcca gctccagtta
    48841 atcgggatgt ggaaattgca gatagtttgg tggaagctcc taaagcgaga attgtatctc
    48901 agatggctaa tggtgtcttt gttcgcatgg ctattattga agccattttg aatggtagaa
    48961 ataaaaactc atgatgaaag attaaagcat gtttagaagg taggaaaagg taggaaaatg
    49021 acaaaacgat tacttatttt agaagacggc actatttttg aaggagaacc ctttggtgct
    49081 gatattgatg tgactggtga aattgtcttt aatacaggaa tgacaggcta ccaagaatcc
    49141 atcactgacc aatcctacaa tgggcaaatt ttaaccttta cctatccttt gattggaaat
    49201 tatgggatta atcgagatga ctatgagtcc atttcaccca cctgtaaagg ggttgttgtg
    49261 tctgaagtca gtcggttggc aagtaattgg cgcaagcaaa tgacgctaga tgcgttcttg
    49321 aaaataaaag ggattccagg gatttcggga attgatacga gagctttgac caaaattatt
    49381 cggcaacacg gtaccatgaa agctacgatg gcagatgatg gagatagtat ccagcattta
    49441 aaagatcaac tgcgtgcaac agtcttaccg actaacacca ttgaacaagt gtcaactaaa
    49501 acagcttatc ctgcaccagg gatcgggaaa aatattgtat tggttgattt tggtttaaaa
    49561 cattctattt taagagaatt ttctaaaaga caatgtaata tcaccgttgt ccctttcaac
    49621 atcactgcag aagaagtgct ccaactaaat ccggatggtt tgatgctatc taatggccca
    49681 ggaaacccag aagatttgcc agaggcttta gacatgattc gtggggtaca aggtaagatt
    49741 cctatttttg gtatttgtat gggacatcaa ctctttagtc tagcaaatgg tgccaagact
    49801 tgtaaaatga cctttggtca tcgtggcttt aaccatgccg ttagagaaat tgcaacggga
    49861 agaattgatt ttaccagtca aaaccatggt tatgccgttg aacgtagtag tctacctgat
    49921 actttaatgg ttacgcatga agacattaac gataagacgg tcgaaggagt gaaacataga
    49981 gattttccag ctttttcagt ccaatttcac cctgatgcgg ctccaggacc tcatgatgcc
    50041 agctacttat ttgacgaatt tttggaaatg attgattctt ggagatgtac gagtaaatag
    50101 taaatcagtt gcaatttcac gactatatca cctcttgtta ctctatttat taacatttac
    50161 agttgattta gtaccctatc aaaaatatta tttaatgttg ctctgtgagg caacaaatag
    50221 agaaaggaag gcaaggagaa cacaagtaaa taatttagta aaaagttagg ttttcaaaga
    50281 gataaagctc ttattgagaa gtgctgactt taccgcttct tgtggtattc ttggtatcat
    50341 taattatgcc aaaacgaaaa gatattcaaa aaatcatggt tatcgggtct ggcccgatta
    50401 ttattggaca agctgctgaa tttgattatg cagggactca ggcctgtttg gctctaaaag
    50461 aagaaggcta taaggtcata cttgttaatt caaatcctgc aacgattatg acagataaag
    50521 aaatcgctga taaggtatat attgagcctt tgactcttga atttgttaat cgtatcattc
    50581 gaaaagaaag gccagatgcc atactgccaa ccttaggtgg tcaaacgggc ctcaatatgg
    50641 caatggcatt atctaaagct ggtattcttg atgatttaga gattgaattg ttaggtacta
    50701 agttatctgc gattgatcag gcagaagaca gagacctttt caaacaatta atgcaagaac
    50761 tagaccaacc cattccagaa tctacaattg ttaaaacagt tgatgaagct gttacatttg
    50821 ctagagatat cggttatcca gtcattgtgc gcccagcttt tactcttggt ggaactggtg
    50881 gaggaatatg tagtagtgaa gaagaacttt gtgaaattac tgaaaacggc ctaaagttat
    50941 caccggtcac acagtgtttg attgaacgct caattgctgg cttcaaagaa attgaatacg
    51001 aagtaatgcg agatagtgct gataacgcgc tggttgtttg taatatggaa aactttgatc
    51061 ccgtagggat tcacacagga gattctattg tatttgcacc aacacagacc ctttctgata
    51121 ttgaaaatca aatgttacgt gatgctagtt taaaaataat tcgagctctg aaaattgaag
    51181 gtggctgtaa cgtccagtta gcactagatc cttatagttt caaatattat gttattgaag
    51241 taaatcctag agtgtctcgt tcctctgccc ttgcttcaaa agcaacaggt tatcccattg
    51301 ccaaattggc tgcaaagatt gctgttggcc taacacttga tgaaatgatt aaccctatca
    51361 caggcactac ttacgctatg tttgaaccta cccttgatta cgttgttgct aaaattccgc
    51421 gttttccgtt tgataaattt gaacatggtg aacgccaact gggaactcaa atgaaagcaa
    51481 caggtgaagt catggctatt ggccgtaatt tggaagagtc cttactaaaa gcctgtcgtt
    51541 ctttagaaat tggtgtttgc cataatgaga tgacaagttt aagtaacatt agtgatgaag
    51601 agttagtgac taaagtgatt aaagctcaag atgatcgact tttttacttg tcagaagcaa
    51661 tacgacgagg ttatagtatc gaagaacttg agagcttgac caagattgat ttatttttct
    51721 tggataaatt acttcatatt gttgaaatag agcaagatct acaaatgcat gttgagcatt
    51781 tggaaagcct caaaaaagca aagcgttatg gattttcaga tcaaaaaatt gcagaaattt
    51841 ggcaaaagga tgaaagtgat atcagagcga tgagacattc tcatagcctg tatcctgtct
    51901 ataaaatggt agatacttgt gcagctgaat ttgatgctaa aaccccttat ttttattcaa
    51961 catatgagtt ggaaaatgaa tctgttcagt cgaataaaga atcaatttta gttttaggtt
    52021 caggccctat tcgtattggt caaggggttg aatttgacta cgcaacggtt cattctgtta
    52081 aagccatcca aaaagctggc tatgaagcca ttatcatgaa ttcaaatccg gagactgtat
    52141 ctacagattt ttcggtatca gataaattat attttgaacc gttaaccttt gaagacgtca
    52201 tgaatgtgat agatttagag cagcctaagg gagtaatagt tcagtttgga ggacaaactg
    52261 ctattaactt agcacaagca ctttctgagg cgggtgttac aatattagga acacaagtag
    52321 aggatttgga tagggctgaa gacagagatt tatttgaaaa ggcgttgaaa gagcttggaa
    52381 tcccacaacc acaaggtcaa acggcaacta atgaagaaga agcattagag gcagctaaaa
    52441 aaattggttt tccagtctta gttagacctt cttacgtact tggcggccgg gctatggaaa
    52501 ttgttgaaaa caaagaagat cttagagaat atataagaac agcggttaaa gcaagtcctg
    52561 aacatcctat cttagtggac tcttatattt tcggtaagga gtgtgaagtg gatgccattt
    52621 cagatggaaa atcagtctta attcctggta ttatggagca catcgaaaga gcaggtgttc
    52681 attcaggaga ttcaatggca gtttatccgc cacagcaatt atctaagcaa attcaagaaa
    52741 caatagctga gtatactaaa cgtttagcca ttgggttaaa ttgtattggt atgatgaatg
    52801 ttcaatttgt tattaaaaat gaacaagtct atgttattga agtgaatcca cgcgctagtc
    52861 gtaccgtgcc tttcttatca aaagtaacag gtattccgat ggctcagatt gctactaagt
    52921 tgattttagg tcaaacatta aaggatctag gatatgaaga tggtctttat ccccaaagtc
    52981 aactggtgca tatcaaagcg cctgtgtttt catttacaaa gttggcacag gttgatagtc
    53041 ttttaggtcc tgaaatgaaa tcaactggag aagtaatggg ttcggatact agtcttgaaa
    53101 aagcacttta taaagcgttt gaagccaata attctcattt atcagaattt ggtcaaattg
    53161 tctttactat agcagatgat agtaaggcag aagctctaag tctagcaaga cgatttaagg
    53221 cgattggcta tcaaattatg gctactcaag gtacagcagc ttactttgct gagcaaggct
    53281 taagcgcgtg tttagttgga aaaattggtg atgctgccaa tgacatcccg acactcgtgc
    53341 gacatggtca cgttcaggca attgttaata cagttggaat taaacgcaca gctgataaag
    53401 acggacagat gattagaagt tcagctattg aacaaggagt tcctttattt acagctttag
    53461 atactgcaaa agctatgtta acggttttag aaagcagatg ttttaacatc gaagcgatat
    53521 aaaaattaaa aaatcatttt taagaaaact taaaaatgat ttttttagat tataatgaca
    53581 aaagtttcag taagctatta atttgtcata gcatataagt cattatcttg tggcttgttt
    53641 tcgactaagg gttaagaatt tcttgacaac aatataaaaa ggtcatatac ttgtcttata
    53701 caaatagtac aaaaagggga ggatagaaat gtctaaaaga ggaaaaatta aaataacaac
    53761 gaaaacaaag cttattacag ctagtgttat aacgctagta ttaattataa ctggagtagt
    53821 cttgtggaaa caacaacaaa atacgctaac agctgatatc gctaaagaac cttactctac
    53881 tgttagtgta actgaaggga gtattgcttc ttcgacttta ctctcaggta ctgtaaaggc
    53941 tttatcagag gaatatattt atttcgatgc taataaagga aatgatgcaa ctgttacagt
    54001 taaagtaggt gatcaggtaa cgcagggcca gcaattagtt caatataata caacaacagc
    54061 tcagtcagct tatgatactg ctgttaggag tcttaacaag attggccgac aaattaatca
    54121 tcttaaaaca tacggagttc ctgctgttag tacagaaact aataaagatg aagctaccgg
    54181 tgaagagacg acaacaacag ttcaaccatc agctcagcaa aatgctaatt ataaacagca
    54241 gctgcaagat ttaaatgatg cttatgcaga tgcacaagca gaagtaaata aagcgcagat
    54301 agcgttaaat gatacagtag ttatcagtag tgtctctgga actgttgtgg aagtaaataa
    54361 tgatattgat ccttcttcaa agaacagtca aacacttgtt cacgtagcaa ccgaaggaca
    54421 gcttcaggtg aaaggaacat tgacagagta tgatttagca aacgttaagg ttggtcaatc
    54481 tgtaaaaatt aagtctaaag tttattctaa tcaagaatgg actggaaaaa tatcatatgt
    54541 ttcaaactat ccaactgagt ctaatgcagg ttcaacaacg ccagcaggta gcactggagc
    54601 gggaagttct acaggagctg cctatgatta caagattgat attataagtc ctctcaacca
    54661 gcttaaacaa ggtttcactg tttctgttga ggttgttaat gaagctaaac aggccttagt
    54721 tcctttaacg gctgttatta agaaagataa aaaacactat gtttggactt atgatgatgc
    54781 tactggcaaa gccaaaaaag tagaggtgac acttggaaac gcagatgcac aacaacaaga
    54841 aattcataaa ggagtagctg ttggtgacat tgttattgcc aatccagata aaaatatcaa
    54901 accggataaa aaactagaag gggttatttc aataggtacc aacacaaaac cggaaaaaga
    54961 ttctcaatca aagaataaaa aatcaggggt ggataaatag tgtcgcatga aaaaaaacaa
    55021 ttaatgcaat tatcaaatat tgtgaagagc tatcaaaatg gggatcaagt tcttaaagtt
    55081 ttgaaaggta ttaatttaac agtatatgag ggtgaatttt tagctattat gggtccgtct
    55141 ggctcgggaa aatcaacact aatgaatatt attggtttat tagatcggcc aacatccgga
    55201 gattacacat tacataatac taaaatagaa atccttaatg atcgtgaatt agcaaaagta
    55261 cgaaacgatg aaatcggctt tgtctttcaa caattctttc tgttagctaa attaactgca
    55321 ttacaaaatg ttgagttgcc tcttatttat gctggtgtaa acgtctcaaa aagacgtgaa
    55381 caggctaagc aatttcttga aaaagtagga cttggacgac gtatcaaaca tctaccgtca
    55441 gaactgtcag gaggtcaaaa gcaaagggtg gctatcgcac gagctttagt aaatgaccca
    55501 tctattattt tagcggatga accaacaggt gcactagata ctaaaacagg tcagcaaatt
    55561 atggaactct tgacagagtt aaacaaagaa ggtaaaacaa ttattatggt tactcatgaa
    55621 ccagaaattg ctgattttgc aactcggaaa attattattc gtgatggtga tatcacaacg
    55681 gatactacag ctagtgttgt tattgactag gaggaaaaat aatggaaaat tggaaattcg
    55741 ctcttagctc aatttggggg cataagatgc gctcaatcct gacaatgttg ggaattatca
    55801 tcggtgtagc agcagttgtg attattatgg ggttgggaaa tgctatgaaa aatagtgtaa
    55861 caagcacatt ttctagcaag caaaaagata ttcaactcta ttttcaagaa aaaggagaag
    55921 aagaagacct gtatgccgga ttgcacacgc atgaaaataa tcacgaagtg aagccagagt
    55981 ggttagaaca aattgttaaa gatatcgatg gtattgattc ttattacttt accaattctg
    56041 ctacttctac tatttcgtat gaaaaaaaga aagtagacaa tgcttcgatc ataggggtaa
    56101 gtaaagatta ttttaacata aaaaactatg atatcgtagc tggtcgtaca ctaacagata
    56161 atgattattc taatttttcg cgtatcattc ttttagatac agtattagcc gatgacctat
    56221 ttggcgaagg taattataag tcagctttaa ataaagtggt aagtttatct gataaagatt
    56281 atttagttat tggtgtttat aaaactgatc agacgccagt ttcttttgat ggattatcag
    56341 gcggcgctgt catggcaaat actcaggtag cttcagagtt tggcaccaaa gaaataggat
    56401 ctatctacat tcatgtgaac gatattcaaa acagtatgaa cttaggaaat caagcagctg
    56461 atatgctaac caacatatcg catattaaag atggacagta cgctgttcct gataatagca
    56521 aaatcgttga agaaatcaat agtcagttca gtatcatgac aacagtcata ggttcaattg
    56581 cagcgatctc tcttttagtg ggaggaattg gtgttatgaa tattatgttg gtatctgtca
    56641 cagagcgaac tagagaaatt ggtttacgta aagcacttgg tgcaacacgc ctgaaaattt
    56701 tatcacaatt tttgattgaa tccgttgttc taacagttct tggaggcttg ataggattat
    56761 tattagctca attaagtgta ggggctttag gaaatgcgat gacattaaaa ggagcttgta
    56821 tttctcttga tgtggcctta atagcagttt tattttctgc ctcaataggt gtcttttttg
    56881 gaatgttgcc agctaataaa gctagtaagt tagatccaat tgaggcatta cgttatgaat
    56941 agaatagttc gtatcaatta atctatagac gctgactagt ttaacgtctg attatatcta
    57001 gtttttgtat tagcacagta gttatatcat tgaagtgtta gtgcattttt tgaggtgata
    57061 atatctagca aagctatcat tggtatgatg atgtggatag cgtataagtg atacatctct
    57121 tatttctagc tgaacttcgg ttcggctagg aagttgtcag gcaatgagtt gtttaaaata
    57181 aaggagatta gttcaatgac atttttatct gatttgatat cattaatgac aaaaatcaga
    57241 ttatcttggg taataaaggc gggtattttt caattattat ttgtaacgat tgctaatatt
    57301 gtcttatcag aatttttcta ttttatatta gacgttactg gtcaatatca tttagataaa
    57361 gacaatgttg tgactttttt aaaaaatcct atagcacttg ctttattagg tgcctattta
    57421 tttttattag ctgcttttat tcaccttgag ttttttgctc tatatcgaat tattgcggat
    57481 caagaaatta gtttctatct ttttagaaaa cagttttctt attacctaag ggggctttgg
    57541 aaaacatttt ctggttacca attattactt tttttgcttt atatcctatt gactattcca
    57601 gtcttacata ttggtttatc ttctgtgatt actcaaaagc tttatcttcc agaatttatt
    57661 gttggggaat tatcaaagat aactagcaca aagtacttgc tttatggcag tcttattctt
    57721 gtattttacc ttaacctaag attagtatat tttttaccat tgatagcaat caaccatcgt
    57781 acggttgctc aagcatggag agagagttgg caaaagacta aaaagaaaca tgtattgtta
    57841 tggatgaaac tttttgcaat caatggtctt acgcttgtag tcgtatcgct agctatttcc
    57901 atgattctta tttttgttga tatgtttaat cctaagggga ataatattat tgttcagctg
    57961 ggagctttga cctttacatg ggagctcatt ttttttacta ctattttttt taaactctgt
    58021 tcagcaatga ttttaaaaga ggcaattgaa ccacaaaagc aatatgatga gccaagaaga
    58081 agtaataagg catatgttgt aatctttatc gtggtgacag taggttttgc ttatcaatct
    58141 cttgaacgtt taactttttt tgacacatct cactctaaga cagttatcgc gcatagagga
    58201 cttgtatcag caggtgtaga aaattctctg gaagcccttg aaggtgctaa gaaagcagga
    58261 agtgattatg tagaactgga tctaatcttg actaaggata atcactttgt ggtgtctcat
    58321 gataatcgat tgaagcgttt agctggagta aataagacga ttcgcaactt aaccttaaaa
    58381 gaagttgaac atctaacgag tcatcaagga catttttcag ggcgttttgt ttcttttgac
    58441 actttttatc aaaaggctaa gaagttgaat atgccattac ttattgaact caagccaatt
    58501 ggtacagaac ctggaaatta tgtcgatttg tttttagaaa cttatcatcg acttggtata
    58561 agcaaagata ataaagtcat gtctttagat ttagaagtaa tagaagctat caagaaaaaa
    58621 aatccatcaa ttacgactgg ttatatcata ccaattcaat ttggcttttt tggagatgaa
    58681 tttgttgatt tctatgtcat tgaagacttt tcttatcggt cttatttgtc gtcccaagct
    58741 ttttggaata ataaagaaat ttacgtttgg actattaatg atcccaagcg catagagcat
    58801 tatctcctaa agcctattca gggaattatt acagatcaac cagctttaac taatcaattg
    58861 attaaagact taaaacaaga taattcttat tttagtcgat tagtcagaat tattagtagt
    58921 ctttattaaa ggtttaaaga aagtgtcaag aaaaaaactt gacactttct ttaaactgag
    58981 ttatactgtt taacatagga atttagcgta gctgtatttc ttatacatta ctaaaagaaa
    59041 agagactatt taaaaatggc agtaaaaatc cgtttaactc gtatgggttc taagaaaaaa
    59101 cctttctacc gtattaatgt tgcagattca cgtgcaccac gtgatggtcg ttttatcgaa
    59161 acagttggaa catacaaccc attagtggct gaaaatcaaa ttactattaa agaggatcgt
    59221 gttcttgagt ggttatctaa aggagcgcag ccttctgata ccgttcgtaa catcttgtct
    59281 aaagctggtg ttatggctaa gttccacgac caaaaattct ctaaataata acagcctatg
    59341 gataccattg aaaatcttat tatcgctatt gtgaaacctt tgatttcaca accagataat
    59401 cttaccatta aaattgaaga tactcctgac tttttagagt atcatcttga cttggatgct
    59461 caagacatcg gtcgtgttat cggcaaaaaa ggtcgaacta ttactgcgat aagatcgatt
    59521 gtctactcgg ttccaacact aggaaaaaaa gttcgcttag ttattgatga gaaataatca
    59581 ctaacttgag atggattgaa aagcttgttg acttaaagtc aacaagcttt ttgaatagca
    59641 aaaatcttaa tatttctgaa atgaaaagat tagatttttc tgtctttaac taaaaagaag
    59701 ttaaagaatg gcttagctga ttgcctttat aagagattat actggttttt gatagctgct
    59761 gtcatgtaaa atagctacaa ttcatcttac gagattgacc ttagcattgc cagtgcagtc
    59821 tttattttct aacctgctgt ttgttgtatg tttatagttt cctaaaaaat gctctctcag
    59881 ttttttgggg gacattccta aaattaagta tacttaattt taggaatgtt tatattcttt
    59941 acattctgat gaaaatacaa attatcctaa catgatgtat tgaaaggaga aatagtttat
    60001 gttagtatac tgattaagtg aagctgtaat gaggcttata cgatcaaaaa agttagagta
    60061 actttataaa acagtggtgt tgaagcaagt ttagcttctt ctcagaaata gattatctat
    60121 aaaaaatccc aaaagtagtt tgtttccagt taatgaactt cttttgggaa ggcttttttg
    60181 ctgatcgcca cttcacttgt atatcgggaa cattcacgct aagatgtata agttgaatcg
    60241 tttgtctatg gcttgatgaa aattaatcaa aagataatgt tcaactctat aattagtaca
    60301 agcaaaatga gataacttat tcattagtaa gcataagata taacaagaga aattacctta
    60361 aaacaggaag tactagagta tttaaaataa gtgatagact tggatattat taggtcgact
    60421 agtgttcttt tcaataattt aatatcgtta ttattagtat cagccaatca aatgtggata
    60481 agttggcttt tagaaaggtt ttaccacaat gaagaaacat cttaaaacag ttgccttgac
    60541 cctcactaca gtatcggtag tcacccacaa tcaggaagtt tttagtttag tcaaagagcc
    60601 gattcttaaa caaactcaag cttcttcatc gatttctggc gctgactacg cagaaagtag
    60661 cggtaaaagc aagttaaaga ttaatgaaac ttctggccct gttgatgata cagtcactga
    60721 cttattttcg gataaacgta ctactcctga aaaaataaaa gataatcttg ctaaaggtcc
    60781 gagagaacaa gagttaaagg cagtaacaga gaatacagaa tcagaaaagc agatcacttc
    60841 tggatctcaa ctagaacaat caaaagagtc tctttcttta aataaaacag tgccatcaac
    60901 gtctaattgg gagatttgtg attttattac taaggggaat acccttgttg gtctttcaaa
    60961 atcaggtgtt gaaaagttat ctcaaactga tcatctcgta ttgcctagtc aagcagcaga
    61021 tggaactcaa ttgatacaag tagctagttt tgcttttact ccagataaaa agacggcaat
    61081 tgcagaatat accagtaggg ctggagaaaa tggggaaata agccaactag atgtggatgg
    61141 aaaagaaatt attaacgaag gtgaggtttt taattcttat ctactaaaga aggtaacaat
    61201 cccaactggt tataaacata ttggtcaaga tgcttttgtg gacaataaga atattgctga
    61261 ggttaatctt cctgaaagcc tcgagactat ttctgactat gcttttgctc acctagcttt
    61321 gaaacagatc gatttgccag ataatttaaa agcgattgga gaattagctt tttttgataa
    61381 tcaaattaca ggtaaacttt ctttgccacg tcagttaatg cgattagcag aacgtgcttt
    61441 taaatcaaac catatcaaaa caattgagtt tagaggaaat agtctaaaag tgatagggga
    61501 agctagtttt caagataatg atctgagtca actaatgcta cctgacggtc ttgaaaaaat
    61561 agaatcagaa gcttttacag gaaatccagg agatgatcac tacaataacc gtgttgtttt
    61621 gtggacaaaa tctggaaaaa atccttatgg tcttgctact gaaaatacct atgttaatcc
    61681 tgataagtca ctatggcagg aaagtcctga gattgattat actaaatggt tagaggaaga
    61741 ttttacctat caaaaaaata gtgttacagg tttttcaaat aaaggcttac aaaaagtaaa
    61801 acgtaataaa aacttagaaa ttccaaaaca gcacaatggt gttactatta ctgaaattgg
    61861 tgataatgct tttcgcaatg ttgattttca aaataaaact ttacgtaaat atgatttgga
    61921 agaagtaaag cttccctcaa ctattcggaa aataggtgct tttgcttttc aatctaataa
    61981 cttgaaatct tttgaagcaa gtgacgattt agaagagatt aaagagggag cctttatgaa
    62041 taatcgtatt gaaaccttgg aattaaaaga taaattagtt actattggtg atgcggcttt
    62101 ccatattaat catatttatg ccattgttct tccagaatct gtacaagaaa tagggcgttc
    62161 agcatttcgg caaaatggtg caaataatct tatttttatg ggaagtaagg ttaagaccat
    62221 aggtgagatg gcatttttat caaatagact tgaacatctg gatctttctg agcaaaaaca
    62281 gttaacagag attcctgttc aagccttttc agacaatgcc ttgaaagaag tattattacc
    62341 agcatcactg aaaacgattc gagaagaagc cttcaaaaag aatcatttaa aacaactgga
    62401 agtggcatct gccttgtccc atattgcttt taatgcttta gatgataatg atggtgatga
    62461 acaatttgac aataaagtgg ttgttaaaac gcatcataat tcctacgcac tagcagatgg
    62521 tgagcatttt atcgttgatc cagataagtt atcttctaca atggtagacc ttgaaaagat
    62581 tttaaaacta atcgaaggtt tagattattc tacattacgt cagactactc aaactcagtt
    62641 tagagacatg actactgcag gtaaagcgtt gttgtcaaaa tctaacctcc gacaaggaga
    62701 aaaacaaaaa ttccttcaag aagcacaatt tttccttggc cgcgttgatt tggataaagc
    62761 catagctaaa gctgagaagg ctttagtgac caagaaggca acaaagaatg gtcagttgct
    62821 tgaaagaagt attaacagag cggtattagc ttataataat agcgctatta aaaaagctaa
    62881 tgttaagcgc ttggaaaaag agttagactt gctaacagga ttagttgagg gaaaaggacc
    62941 attagcgcaa gctacaatgg tacaaggagt ttatttatta aagacgcctt tgccattgcc
    63001 agagtattat atcggattga acgtttattt tgacaagtct ggaaaattga tttatgcact
    63061 tgatatgagt gatactattg gcgagggaca aaaagacgct tatggtaatc ctatattaaa
    63121 tgttgacgag gataatgaag gttatcatgc cttggcagtt gccactttag ctgattatga
    63181 ggggctcgac atcaaaacaa ttttaaatag taagcttagt caattaacat ctattcgtca
    63241 ggtaccgact gcagcctatc atagagccgg tattttccaa gctatccaaa atgcagcggc
    63301 agaagcagag cagttattgc ctaaaccagg tacgcactct gagaaatcaa gctcaagtga
    63361 atctgctaac tctaaagata gaggattgca atcaaaccca aaaacgaata gaggacgaca
    63421 ctctgcaata ttgcctagga cagggtcaaa aggcagcttt gtctatggaa tcttaggtta
    63481 cactagcgtt gctttactgt cactaataac tgctataaaa aagaaaaaat attaaattga
    63541 tttaaggaga agaaatgata acacaagaaa tgaaagatct tattaataac caattagcaa
    63601 tggttgcgac ggttgatgcc aaaggtcaac ccaatattgg tccaaaacgt tctatgcgtc
    63661 tctgggatga taaaacattt atttataatg aaaatacaga tggtcaaact cgtattaaca
    63721 ttgaagataa tgggaaaatt gaaattgcct ttgttgatcg tgagcgcctt ttaggttaca
    63781 ggtttgtagg aactgctgaa attcaaacag aaggtgctta ttacgaagca gcgaaaaaat
    63841 gggcacaagg acgcatgggg gtaccaaaag cagttggtat tattcatgtc gagcgtattt
    63901 ttaatttaca atctggtgct aatgcaggta aagaaataaa cagtgaaagt aactaaaatt
    63961 aactaaaatt aactgtaaca gtatttagga cccttatttt aaatgctaaa aaacaagcat
    64021 actctttttt acagcatagt gactatgttg taaaaagggg tatgcttatt aggtgctctt
    64081 aatatttcta gataactgca cctaataagt tttcaataga cttatagcac attagttgac
    64141 ttcaattagt tgacttcaat accagaaaga gaagaacagt gatgttggtg ttcgtttaaa
    64201 gaagtatcga tttccacagt gactgagttt atattgtacc tttgacaaat cagtctaata
    64261 gattttttgc aatgtttttc agtggtagat tctttcaagc agcaatgtat agttgctcta
    64321 tggtcaatac catccatcga ccaaacatta agctgattaa cagacactat atgaggaagt
    64381 tggctgagtt catggtgcaa agcgcaatag tctatcgaat caggaacacc atctaaaaaa
    64441 atgttagcgg ttgctactaa ctttggaaga gctttagata atataaaaga agcaatggca
    64501 attgataata aaggatcaag aatgtaccaa ggtttccatt ttaagatcag tgacagtatg
    64561 ataatggcta accatccaag gatatcttct aaaaagtgaa ggcttaatat ttcttcattt
    64621 tttgtctggt tagagtgaat gatgaagcta gcaaatccat tgattataat tgcaaaaata
    64681 gctaaaacga acataccgtc ataattaaca atagtaggat gccataattt tggtatcgtt
    64741 tcaatcatta ctaataatga cccgctaata agtattagat ttgtcgttag cgctcctaaa
    64801 aggctaaatc gcttatagcc tagtgaaaaa tttggactct cttttttaac agcctttctt
    64861 tctaagatag ctgagatacc aatagcaatg gcatctccaa aatcgtggac agcatctgct
    64921 aaaatagcac ccgaaaagaa taatgttcca aaaataaatt caatgaggga aaaactaaga
    64981 tttaatataa aaataatggt gactttttta cttgctggca taattttctc cttttgttat
    65041 actgataata tgtatagtag aattctttat tctattatct aaaagttaaa atacaattac
    65101 tataaccaaa aatgagcacc tgttcaatag tgaacaacat ttagaaagtg tccgatatga
    65161 caaaagacag acaaatcaaa aaaacaaaaa cagctatcta tagtgctttt cttgctttat
    65221 tacaaaaaaa agaatattcg aaaataactg ttcgagatat gattacactt gctaatgtag
    65281 ggagatcaac cttttatgca cattatgaaa gcaaagaaat gttactaaaa gaactctgtg
    65341 aagaactttt tcatcatttg tttaggcaaa aaagaaatgt gacttttgag gactatttgg
    65401 tacatatttt aaagcatttt gaacaaaata aagacagcat tgcgacgttg ttactttcta
    65461 atgatcccta ttttttatta agatttaaaa acgaactaga acatgatgtt taccctaact
    65521 tgcgctgcaa gtatatcgac aaaacgacta ttccagaagt attcctgaaa caatttgttc
    65581 tatctagttt tatagaaacc ttaaaatggt ggctccatca aaggcaaagg atgtctgcaa
    65641 atgaactatt aaagtattat ttagagctca taaaataatg actagtgagt catcttgcca
    65701 aattcaattt tttaatacag acggatttta acaatttaac agagaaagac aaaactgtaa
    65761 gtgcaaaata gactataacg tgataaaata ataggtaatt tggttctgaa aaagaagtgt
    65821 ctatcaaact ttagaatcaa gagaggtcgc taaagtgacg tgagataaag agttaacgat
    65881 ttaggagcta ccatggaata ttttaatgtt ggaaaaattg tcaacacgca aggcctgcaa
    65941 ggagagatgc gcgtgttgtc tgtcagtgat tttgctgagg agcgctttaa gaagggatcg
    66001 caattggctt tgtttgatga caaagaccag tttgttcaag aagtgacaat tgttagtcac
    66061 cgtaaacaaa agcattttga tattatcaaa ttgaaagaca tgtatcatat taatgcgatt
    66121 gaaaaatata agggctatac gttaaaagtg tcaaaagcta atcaaggaga cctgcaagaa
    66181 ggagaatttt actaccatca gattatcggt atggctgttt atgaaaagga ccggttgatt
    66241 ggctatgtca aggaaatctt gcagccaggt gctaatgatg tttgggtagt gaaacgccaa
    66301 ggaaaacgag acttgctctt accatatatt cccccagtag ttcttaacgt agatgttcct
    66361 aacaagcgcg tagatgttga attgatggaa ggcttagacg atgaagattg atattttaac
    66421 cctttttcct gaaatgtttg cccccctaga acattccatt gtaggcaaag caaaagaaaa
    66481 aggattactt gacattcact accataactt tcgagattat gctgaaaaag ctcgtcatgt
    66541 ggatgacgaa ccctatggtg ggggccaagg catgttattg agagcacagc ccatttttga
    66601 caccattgaa cagattgaag ccaaaaaacc aaggattatt cttttagatc cagcaggtaa
    66661 accctttact caggcttatg ctgaagaatt agctcttgag gaagagttaa tttttatttg
    66721 tggtcattat gaaggttatg atgagcggat taagaccttg gtgacggatg aaatttcatt
    66781 aggtgacttt gtattaacag gaggtgagtt ggcggccatg actatggtgg atgcgactgt
    66841 caggttaatt ccacaggtac ttgggaaaga aagtagccat caggacgatt ctttttcatc
    66901 aggactttta gaataccctc aatacacaag accttatgat tatcgtggta tgacagttcc
    66961 agatgttcta atgagtggtc atcacgaacg tattcgcttg tggcgtctag aagaaagtct
    67021 cagaaaaacc tatttgagaa ggccagattt gttagagcgg tacgattttt ccgaggaaga
    67081 gcgcaagcta ttggataaga ttaaagaagc attgggtcaa ggagaagatt agtgaaagat
    67141 aaagcatatg atattacgat tattggtggt ggtccaatag gtttatttgc agccttttat
    67201 gcaggtctta ggggcgtaac agtaaagatt attgagagtt tgtcagaact tggtggacag
    67261 cctgccattc tttatcctga gaaaatgatt tatgatattc cagcttatcc atctttaaca
    67321 ggtgttgagt tgacagaaaa tttaataaaa caattaaacc gttttgaaga tcgtataacc
    67381 atttgtttaa aagaagaagt attaaccttt gacaaggtta aaggtggttt ttcgattagg
    67441 actaataagg cagagcattt ttcaaaagcc attatcattg cttgtggaaa tggcgctttt
    67501 gcaccaagaa ctttagggtt agaaagtgag gaaaactttg ctgatcataa ccttttttat
    67561 aatgttcacc agttggacca gtttgcggga caaaaagttg tgatttgtgg tggtggagat
    67621 tcagcagttg attgggcttt ggctttagaa gatattgctg agagtgtgac ggttgtgcac
    67681 cgtcgtgatg ctttcagagc acatgaacac agtgtggaat tattgacaac atcaacggta
    67741 aacctcttaa caccttatgt gccaaaagca ttgaaaggaa ttggaaacct ggctgaaaaa
    67801 ttagttattc aaaaagtgaa agaagatgaa gtgttagaac ttgaattgga cagtcttatt
    67861 gttagttttg gtttttcaac ttctaataag aatttgaaaa attggaatct cgactataag
    67921 cgttcaagta ttactgtttc accacttttt caaacaagtc aagaaggtat ttttgccatt
    67981 ggtgatgcag ccgcttataa tgggaaagtg gatttgattg ctactggttt tggcgaagca
    68041 ccaacagcag ttaatcaggc cattaactat atttatccag atcgagataa ccgtgtggtg
    68101 cattcaacct ctttaattga ctaagggagt cggaagtttc aagagcagta gcaacagcta
    68161 atattcagtt tgtcaggaga ggacaaatgg ttttttacct aagtcttcgt gacaagtttt
    68221 atactttgtg ataaaatagt cattgtgaaa aaagtgtgat ggttttcaca gattaacaca
    68281 ttacaatgaa aaggggtcta tcatgacaac aacagataaa gagacattta gttcttttat
    68341 gaataaggtc ttggctggta cagcgattgc tatcgttgtg gcactcattc caaatgctat
    68401 tttagcgact ttcttaaaac cgcttttgcc aaacatggca gctgctgaat ttttacacat
    68461 tgtgcaagtc ttccaattct tcacgccgat tatggctggt ttcttgattg ggcaacaatt
    68521 taagtttaat cctatgcaac agttggctgt tggtggagct gcttatatcg gttctggggc
    68581 ttgggcttat acagaagtca ttcaaaaagg tgtggcgaca ggaacattcc aacttagagg
    68641 tattggggac ttaatcaata tgatgattac agctagcctt gcggttttag ctgtgaaata
    68701 ttttgggaat aaatttggct ctttaaccat tatcttgtta cctatcacta taggaactgg
    68761 cgtaggttat attggttgga aatttttgcc ttatgtgtct tacgtgacaa ccctgatcgg
    68821 acaaggtatt aattctttta caaccttaca acctatttta atgtctattt taattgcagt
    68881 tgctttctcg cttatcattg ttagtccgat ttcaactgtt gcaatcggtt tagctattgg
    68941 gcttaatgga atggctgcgg gagcagcttc tatgggaatt gcatcgacag cagcagtctt
    69001 agtgtgggcg acgcttaaag ttaataaatc tggtgtacca attgctatcg cacttggggc
    69061 tatgaaaatg atgatgccaa acttcttgaa acatcctatc atggctattc cgatggtgtt
    69121 cactgcagca attagctcat taacagtacc attgtttaat cttgttggaa caccagcttc
    69181 atctggtttt ggcttagttg gtgcagtagg tccgattgct tctttagcag gtggtagctc
    69241 aatacttatt attatccttg cttggatcat tgttccgttt gcggttgctt ttgcggcgca
    69301 taaggtttct aaagatattc taaaacttta taaagaagac attttcgtct ttgaaggcta
    69361 ataggaggaa aatatgttag tttatattgc tggctcagga gccatggggt gccgttttgg
    69421 ttaccaaatt tcaaaaacaa ataacgatgt gattttgtta gataactggg aagatcatat
    69481 caatgccatt aaagaaaacg gtttagtggt gacaggggat gtggaagaaa cagttaagct
    69541 tcctattatg aaaccgactg aagccactca agaagccgat ttgattatct tgtttacaaa
    69601 agcaatgcag ttgccacaaa tgcttcaaga cattaaaggc attattggaa aagaaaccaa
    69661 agtgctttgc cttttaaatg gtcttggaca cgaagacgtt attcgtcagt acattccaga
    69721 gcacaacatt ttgatggggg tcactgtctg gactgctggt ctagaaggac caggtcgtgc
    69781 tcatcttcaa ggagttggtg ctcttaactt acaaagtatg gatccaaaca atcaagatgc
    69841 gggacatcaa gtagctgact tgttaaataa agctaactta aatgcgactt atgatgagaa
    69901 tgttgtgcca aacatctggc gaaaagcttg cgtgaatggt accatgaatt cgacttgtgc
    69961 tcttttagat tgtacgattg gcgaattatt tgccagtgaa gatggtctca aaatggtaaa
    70021 agaaatcatc catgagtttg ttatcgttgg tcaagcagaa ggtgtcgaac taaacgaaga
    70081 agaaatcaca caatatgtaa tggatacatc tgttaaagct gctcaccatt acccatcaat
    70141 gcaccaagac cttgtgcaaa atcaccgatt gactgaaatt gattttatca atggtgcagt
    70201 caataccaaa ggtgaaaagc ttggtatcaa tactccatac tgccgtatga ttacagaact
    70261 agttcacgct aaagaagcag tgttgaacat ccaataaacg ctatcaaatc gttatactca
    70321 atattatggc taaagcttta atattgagtt ttttgttatg ctgatagtat ttttcttagg
    70381 cgtcattatt tgtgagccta gattatgagt taataaaaga aataataatt ggtagaattt
    70441 gatagatttt gaaaggttat gcttgacttt tagttataaa atagtataat aattgtaaac
    70501 gatttcagtt aggggtggtt ctatcttaaa atcaaaacga aaacaattaa tcatggcaaa
    70561 gatcactgaa gaaaactatg tttctttaga ggatttaatg caattactca actcctctga
    70621 gtctactatc cgtagagatc taggagagtt ggagcaagaa ggacggctac atcgtgttca
    70681 tggaggtgct gaactttttc actctttaca agaggaactt tctaaccaag aaaagtctgt
    70741 caaaaacagt catattaaaa aaactattgc gcaaagagcc tctcagttta tctatgacaa
    70801 cgatgtgatc tttattgatg ctgggacaac gactgaattt ttattacctt ttttacaagc
    70861 taaaaattta actgtagtta ctaattctat tcatcatgct gctagattag ttgaactctc
    70921 tattgagacg attattgtag gtggttatgt caaacaaacg accgatgcca gtattggtaa
    70981 tgtagctttg gaacagatta ggcagatgaa ttttgacaag gcatttcttg gaatgaatgg
    71041 tattgatgat tcttacctga cgactcctga tatggaagaa gcagttatta aaaaagcggt
    71101 tctgtcaaat gcaaaattag cttacatttt agtagatggc actaaaattg gtcaggtatc
    71161 gtttgtaaaa gtagctccta ttaatgatgt gactattatt acattaggag gatcagcaag
    71221 tattcttaaa caaataaagg aaaaggcaaa ggtgattgaa ttatgattta taccgtgacc
    71281 ttaaaccctt ctattgactt tattgtcaga attgaccaaa taaatcttgg gtcagtgaat
    71341 cgcatggcga gtgatgataa gtttgctgga ggtaaaggca ttaatgtgag tcgcatcttg
    71401 caacgtttag acattgctag cacggcgaca ggattcttgg gtggttttac aggacgtttt
    71461 atcgaagaaa gtttgagtgc agaaggcgtt aaaactgatt ttgtaaaagg tgatcaagat
    71521 actcgaatca atgttaaaat caagtcacaa gaagagactg aaataaatgg tcaaggacct
    71581 gtcattagtc gggaacaact agaggatttg aaaactaagc tttcccagct aacagcagag
    71641 gatacagttg tctttgcagg ttcagcgcca gctaatcttg gcaatgctgt ttataaggaa
    71701 ttatttcctt tagttaggca aagcggtgct caggtggttt gtgattttga aggacagact
    71761 ttaatagatg cgcttacata taatcctctg ttggttaaac ctaataatca tgaactagaa
    71821 gctatttttg gtacgattct gactagtctt gatgatgttg aaacctatgc ccgtcgtctt
    71881 ttagagatgg gtgctcaaaa cgtcatcatt tcaatggctg gtgatggtgc tttgctggtt
    71941 actaaagaag cgacttattt tgctaaaccg attaagggag aagttaaaaa ttctgtcggt
    72001 gctggtgatt cgatggttgc tggctttaca ggcgaattta tgaagagtca aaatccaata
    72061 gaagccttaa aatggggcgt ggcttgtggt acagcgacgg ctttctctga tgacttggcg
    72121 acaattgcat ttattaagga aacatatcat aaagttgagg tagaaaaacg atgaaaattc
    72181 aagatttatt gagaaaagat attatgattc tcgatttaca ggctatttct aaagaagttg
    72241 ccattgacga gatgattact aaattagttg aaaaagatat tgtacatgat tttgatgtct
    72301 ttaaaaagag tatcatgaca cgtgaagaac aaacatcaac agggcttggt gatgggattg
    72361 ccatgcctca ttctaaaaat attgtagtag ataaaccagc ggtcttgttt gcgaaatcaa
    72421 ataaaggtgt ggattataag gctttagatg gtcaaccaac ggatctcttc tttatgattg
    72481 ctgcgcctca aggagcaaat gatactcact tggctgcgct agcagaatta tcacaatacc
    72541 ttttgaaaga cggctttgct gataaacttc gtgctgcagc gacaccagaa gcagtcattg
    72601 ctgtttttga tgaagcgtca acagctaaag aagaagtggt tgctccaaca agtggtcaag
    72661 actttatcgt tgctgttaca gcttgtccga caggaattgc tcacacttat atggcagaag
    72721 aggctttgaa aaaacaagct gcagaaatgg gagtagctat taaagttgaa acaaacggtg
    72781 cttctggtgt ggctaatcgt ttaaccgctg aagatattca gagagctaaa ggagttattg
    72841 ttgcagctga taaagcggtt gaaatggacc gttttgatgg taaacaattc attgctcgcc
    72901 ctgttgcaga tggtatcaag aaaagtcagg aattgatttc tttgatttta aacaatgaag
    72961 gaaacactta tcatgctaaa aatggaaaat ctgaaacagc agtatcaact gagaaaacaa
    73021 gtttaggcgg tgctttctat aaacacttga tgggcggtgt ctcacaaatg ttgccatttg
    73081 ttattggtgg cgggattatg attgctctag catttttatt ggataacatg cttggtgtgc
    73141 caaatgatca gcttggaagt cttggatcct atcatgaaat agcagctatt tttatgaaca
    73201 ttggtggagc agccttttcc ttcatgttac cagtactagc aggttatatc gcttattcta
    73261 ttgctgaaaa accgggctta gtagctggtt ttgtggcagg tgccattgcc tcaaatggtc
    73321 ttgcttttgg taaggtacca tttgcagcag gaggagaagt gagcttggga ttaacgggcg
    73381 tgccatcagg tttccttggg gcgcttgtgg gtggtttcct tgccggtggt gttattcttg
    73441 cccttcgtaa attgttggca ggcctaccac gttctctaga aggggttaaa tctatcctcc
    73501 tttacccatt acttggcgtc cttgtcactg gtttcttgat gctttttgtc aatattccaa
    73561 tggcagctat taacacagct cttaatgatt tcttacaagg tctttcagga agctctgctg
    73621 tccttatggg acttcttgtt ggtggaatga tggctgttga catgggtggt cctgttaata
    73681 aggcagctta tgtttttggt acaggaactt tagcagctac tgtggcaaat ggtggttcgg
    73741 tcgttatggc cgctgttatg gcaggtggta tggtacctcc tcttgctgtt tttgtagcca
    73801 ctctcttgtt taaagataaa tttaccaaag aagaacgcga atcaggtttg accaacattg
    73861 tcatgggact ttctttcatc actgaaggag caatcccatt tggcgcagct gacccagcgc
    73921 gtgcaattcc tagctttatt gctggttctg ccttgacagg agctcttgtt ggtttggctg
    73981 gcattaaatt aatggcgcca catggtggta tcttcgtaat cgctttaaca agcaatccaa
    74041 tcttatatct tgtctttgtt gtgattggtg cccttgtatc aggtatctta tttggcgctc
    74101 ttcgcaaaaa agcctaaaag gtaaagaact tttttcttac taaaactaga atctgtcaag
    74161 gttctagttt tttgctatac tagagttaaa ggataagaaa tgaggacctt tgaaggtgaa
    74221 aaaaaggaga agaagggcta aatcatcagt aaatcgactt gtcttagggt tagtattact
    74281 taatctgatt gttagtatgt ggacactaaa attgggaaac cagcgattag ctccgtatgc
    74341 ggatcacgaa actctgacat ttgtcaggaa gattagtcat gctgctcaat cagtcgctca
    74401 aaagaaacag ttatacagtt cggtgatgat ggctcaggcc attttagaat ccaataatgg
    74461 caagtcacaa ctaagtcaaa aaccttatta taattttttc gggattaagg ggagctataa
    74521 agaacggtca gtcatttttc caactttaga agacgatgga caagggaatc tttatcaaat
    74581 tgatgctgct tttcgttcct atgggagcct gacagcttgt tttttagatt acgcaagagt
    74641 tttgaatgat ccactttatg acaaaaccca caaaaagttt tggtctcatt atcaagatgc
    74701 tactgcaacc ttaacaggca cttacgccac tgatacaact tacaatacca aattaaatga
    74761 attgattgaa tggtatcaac tcactaattt tgatggtcta atgaaatagt tatccacaat
    74821 tttaaaaaaa atcagaagaa atatgtcgat ttcaagacaa aagttatcaa tctcattaaa
    74881 ttatctgtgg aaaagttaat ttaaacgttc gtgtgctata atagaaacat agaaaatgta
    74941 taaaaaagga tatgtgaata atgacaaaaa agaaaggtaa gcttgtactt atcagtcttt
    75001 ttgttctagc agcttgttta ggagcttata gtgcaatgag acaatctcat aaaacttcaa
    75061 acgtgtcagc tgagactatc gccagttctt caacccgaca ttttattgat gaaattggtc
    75121 caactgctag tactattggt caagaacgtg atctttatgc gtctgttatg atagcacaag
    75181 cgattttaga atcaagtaat ggtaaatcaa gtctaagtca agcaccttat tataattttt
    75241 tcggtattaa gggtgcctat aacggttctt ctgtgacgat gtcaacttgg gaagatgatg
    75301 gtaatggcaa tacctatacg attgatcaag cctttagagc ttatccaagt attgcagact
    75361 cccttaatga ctatgcagac ttgctaagtt ctagcactta tataggtgct agaaaatcaa
    75421 acacgctatc gtatcaagat gctacagcag ctctaactgg cttatacgct acagacacca
    75481 gttataactt aaaactaaat aatattattg caacctatgg tttgacggct tatgatgttg
    75541 ctaatagctc agctcaagaa actggtctag caacttcggg ttatgtttgg aatgagtatc
    75601 gtcgtaatta cactgatgcg gagaccctag cagttgatga ggcttgggct aaacgcatga
    75661 cttattaatg taacactaac aacaatctgc ttgcactgac cccaaaaaag ctggatactc
    75721 tattttaagt aaaggattta gttctgtatt gtacagaact aaatcctttt agttttactt
    75781 tgatatgttt atcgttgtaa taatcaatgt catttgtaat cgcttattct gagtcttcta
    75841 gtgatttaca gttattctca taaccataga acatttctga ttttaagatg ccaaagaaag
    75901 attccatcat gccattgtca ggcctgtttc ttttacgtga catagacgga cgaattcctt
    75961 tagagtttag gaaatgatga taatagttgt gttgatattg ccaaccttga tcactctatc
    76021 atatagttcc gtcatagcat ttatttggag atgccttttt ccattattgg tttaatgtga
    76081 tttaagcaag gttattagct aaacttaatg ccagacaaaa acaccccaat tattagattt
    76141 tttgtctaac ttttggggcg caattcaatc tgagcagttt tagatttggt atcaatgtta
    76201 ttataggtca attgatcata tttatccaga gcttttagct tatttagtcc aaagataaat
    76261 atcaaaatgg ctgaaatctt tcatgtttat agtcttattg tctttatatt tttttaagac
    76321 atcagatctg gtgctattgg gttttgcgtc atataaattg attttttcat gtttcctatt
    76381 tttactgtga atttctaaac tcccccttat ataaggagac ttagtcaaat gaatttcttt
    76441 tgattccatc aaaaattttc ttattttaaa atcgaactct tgaatcgtaa caatatcctt
    76501 atcgattttt ataggattta ttaatgtttt ttgctgttgc ccatctataa aaaaattccc
    76561 tgcaatagta ttagtcctta gaataccatc atctgttgaa tgtaatcttt cttattgata
    76621 cgctttaatt ggtctgattc caactatctt gccctgttga aagtgtaaat aaccctagta
    76681 cttgagcacc aatattatct tcttttattt ctttagcaga aatagctact tttccagcgg
    76741 agttaacacc aacaaagtat ttcttcatac caatagatgc attactatca gagtctgtta
    76801 cataaatagc tttaaggttc ccgttagaat caaagtcagc tccccacagg tttataacat
    76861 ggttgatgcg tacgttagcg caggtgtgtg ataggcctag agccttgcct tcggttaact
    76921 ctttcttaat gagatcactg atttctttga gatttttttc tttaaaatca tgacgacttg
    76981 tcaatagctt actttgatca cctcttgtaa atacggcgtc aaaaatacca cctcggcgat
    77041 ctttctcttg ataggttcta ttgacatcag tagtctgtgt cttataaaca tttaagtaat
    77101 aaccattgat aaacatatct aaagcaagat caggcataac tccaattcgg cgtgctgaca
    77161 aaccggggaa agctttatct cggaaataat taaaaagctc gctatttgtt tggtcacctt
    77221 tagtattaat aacttttctt acatccaata attcttgatc accaaacatg attttttgtt
    77281 tatctgggtg tttttttaga tatgcttcaa ttttttcttt gttttgatcg aaccaccagt
    77341 gaagcatatt ccctgctgtg gcagccccgc aaagaagatc gtcttttcca ttgaatgttt
    77401 tggtaatatc ataccatcct tggttagcaa cataaggagc gtgaaaaaca tcttcgcctt
    77461 gagtgaagtt tgctggagga ataactcctt tggtccaaac ggaagtaacg tgataaggtg
    77521 ttacttccga atatataatc tcttgattag cagaaaaact atctgctata acaccacgat
    77581 ctaccgatag agcaaataaa gtcactgctg ccaatactgc agctgaagtt gaatagcatc
    77641 tttttctcat aagaatacct ttttctatat aattttatta taaaaagatt ataacatata
    77701 gacatctatt gttgcaattt tttaaaataa tacttaattt tttaaataat ttttaataaa
    77761 cgtaaacaag cgattgacaa tggagatatc acaagagata cagtagtggt tgtgcttaaa
    77821 atggacggtt ttttgatgat atgttgccgc atgaggaagt gagcaatagg agagctgtga
    77881 tagaagaaag agaggaagaa gcgttgatga aattagacaa ataaaaaaaa agacctcatc
    77941 cagaagaggt cgtggtataa ctggggacga taggttcaat tgataaaaaa tcttgttagc
    78001 ttacgatcta tgttgttttg aatgccttta actcaacatc gttaatggat tttgagataa
    78061 aaaaataaat ccaatagtct ggtactttcc tattttttgt ctgttaaaac gtttttattt
    78121 tataatggat aaaaggagaa gaagggcgga gcgatgatta agctctatgg gtttaagtat
    78181 gggttagttg gagaggtttt gcggcggatt gaacgcctag gatttacgtt tgagcgatta
    78241 gagctaagac aagctagttc gaaatacttg gctaagcatg atgaggcctt ggtgataaac
    78301 ctttttatcc tggacttaaa acttacctga ccagtggttc tgttttaatt gaggtgcagt
    78361 acaagaccac taacgggtgt ttttttgata taatgtcagt ataaggacga ctaaatataa
    78421 ataggtgaaa aaatagatat ctgttcgaga gatagcactg tttaaaacgt tatgcgaaaa
    78481 tagtagttta ggggctactt taataatctt caaatctatg ctataatgga tcatattgat
    78541 accaaggaga ataaaaaatg caattagttc gtgaaaaaga atttgtcaac cagtaccatt
    78601 atgatgctag aaatttagag tgggaaaaag aaaacggaac accagagacc aattttgaag
    78661 tgacttttca gttgattgat aaagatgagc agcaaaaaga aacagtaatt gtatctgtat
    78721 tgcaatttgt tattgtcaaa gaagaatttg ttattagtgg ggtcatctca caaatggttc
    78781 gtattttaga tcgtttagtg gataaaccaa gcgaatttac gcaagaggaa gttgaatcct
    78841 tagctgctcc tttgctagac atggttaagc gattgactta tgaagtcaca gaaattgcct
    78901 tggatcgtcc aggtattcat ttggagttta aaaattaatg aaattagcag taattactga
    78961 cagtacagca acactaccaa cagatctgaa acaagataaa gctattttta gccttgatat
    79021 accagttatc attgacgatg agacctattt tgaaggtcga aatttgagta tagatgattt
    79081 ttatcaaaag atggctgact cccaaaatct tcccaagaca agtcagccaa gtttatctga
    79141 actagataat cttttaggac ttttgtcttc taagggctat acacatgtta ttggcttgtt
    79201 cttagctgga ggaatctcag gcttttggca aaatattcaa tttttagctg aagaacatcc
    79261 tgagattgaa atggcatttc cagacagtaa gatcacatct gctcctttag gtagtatggt
    79321 aaaaaatgtg ctagattggt cacgtcaagg aatgacattt caagctattt taaacaagct
    79381 gcaagaacag attgatggga cgacagcctt tattatggtt gatgacctta atcatctggt
    79441 taaaggcgga cgcctgtcaa atgggtctgc tcttcttgga aatcttttga gtattaaacc
    79501 tatcttacgc tttgatgaag agggaaaaat cgttgtttat gaaaaagtga gaacagaaaa
    79561 gaaagccatg aaacgcttgg ttgagatttt aaatgacttg atagcagacg gtcagtataa
    79621 tgtgtctatt attcactcaa aagcacaaga caaagctgat tatttgaaac gactcttaca
    79681 agacagtggt tatcaatatg acattgaaga ggttcatttt ggagcggtga ttgctactca
    79741 tttaggagaa ggagccattg catttggcgt aacaccgagg ctttaattgt cgattagaaa
    79801 aaatagttaa ttgcaagaat cactaaaaca ataacaaaat gtgatagaac ccaagtagcc
    79861 taatcacact ttagattggg ctacttggat gataggttag aaaacaacgt atgaaattaa
    79921 tgacaatgcc ttctgagttt cagaaggctt tacccatatt gactaaaata aaagaagctg
    79981 gttatgaagc ctactttgtg ggaggaagcg tcagagatgt tttactcgag cgtcccattc
    80041 atgatgtaga tattgcaact agttcctatc ctgaagagac taaagcgatt tttaacagaa
    80101 ctgtcgatgt tggcattgaa catggtacag tgctcgtgct tgaaaatggc ggtgagtatg
    80161 aaatcacaac ctttaggaca gaagatgtct atgttgacta tcgccgtcct agtcaggttt
    80221 cttttgtgcg ctctttagag gaagacttaa aacgaagaga ctttaccgtc aatgccttgg
    80281 ccttggatga aaatggtcaa gttattgata aatttagagg actcattgat ttggagcaaa
    80341 aacggttacg agcagtgggt aaagcagagg aacgttttga agaagatgca ctacgcatta
    80401 tgagaggctt tcgatttgcg gcgagtctgg attttgacat tgaagctgca acttttgaag
    80461 ctatgaggag ccattcacct ctattggaaa agatttctgt ggaacgttct tttactgaat
    80521 ttgataaatt attgatggca ccgcactggc gaaaagggat ttctgccatg attgcttgtc
    80581 aggcttatga ctatttacca ggcttgaagc agcaggaagc aggcttgaat catctgatag
    80641 tttctttgaa agataacttt acctttagtg atcaccacca agcttgggct tatgtgatga
    80701 ttagtttagc cattgaggat cctaaaagct tcctaaaagc atggaagact tcaaatgatt
    80761 ttcagcgtta tgtcacaaag ttgattgctc tttatagaat tcgtcaggaa agatcttttg
    80821 aaaaattgga tatttaccag tacggcaagg aaatggcaag tctagtggaa ggcctccgaa
    80881 aagctcaaag cttatctgtt gacatggacc acattgaagc tttagatcaa gcgctagcca
    80941 ttcataacaa atacgatatc gtccttaatg gttctcacct gattaaggat tttgggatga
    81001 aaccaggtcc tcagctaggg cttatgcttg agaaagttga attagcgatt gtagagggac
    81061 gcctagataa tgatttcact acaattgaag catttgttag agaggaatta gcaacatgag
    81121 ccattttttg gtgaagaaat tgacaaaaac ggtgggagat aaaacagtct ttcaggatat
    81181 ttcctttatt attcatgact ttgaccgtat tgggattatt ggggttaatg gaacaggaaa
    81241 aacaactctt ttagatgtct tgtcgggaag gcttggtttt gacggagacc attcaccatt
    81301 ttctaaagcc aatgattaca aaatcgctta cttaacacaa gatcctgaat ttaacgatgc
    81361 atcctctgtt ttagatacgg tattgtcatc tgatgttaag gcgattcagc tgattcgtca
    81421 gtatgaacta ttgatggcga attatactga agataaacaa gagaccttag agtccctcat
    81481 gtctgaaatg gatcgcttag atgcttggtc tattgaaagt gatgtcaaaa cagtcctatc
    81541 aaaattaggc atcacggatt tggaacaaaa agtgggagac ctatcaggtg gtatgagaag
    81601 acgtgtccag ttagctcaag tattgctagg agctgctgac ttactcttgt tggatgagcc
    81661 gaccaaccat ctcgatattg acaccatcgc ttggttaaca acctatctca agacagctaa
    81721 aaaaaccgtc ctttttatta cccatgaccg ttatttcctt gatcatgtgg ctacccgtat
    81781 ttttgaattg gataaagcgg gactgacaga atatcaaggc aattaccaag actatgtgcg
    81841 cttaaaagca gaacaagacg aacgagatgc tactaattta cacaagaaga agcaactcta
    81901 taaacaagaa ttagcttgga tgaggacgca gccacaggcg cgtgcaacca aacagcaggc
    81961 tagaatcaat cgtttcactg acttgaaaaa agaagttcac caagacagtt ctgcagataa
    82021 attggagatg gctttcgaaa cgagccgaat tgggaagaag gtgattcact ttgaagacct
    82081 ttcttttgct tatggcgacc gacagttgat taaggatttt aacttgatta ttcaaaataa
    82141 agatcgtatt ggaattgttg gagataacgg cgtcggaaaa tctaccctgt taaatatcat
    82201 caacggtgat ctcaaaccaa cttctgggaa gcttgatatt ggagatacta ttcgtgtagg
    82261 ttatttctct cagcagctca aagatatgga tgagaccaaa cgggtcatta attaccttca
    82321 agaagtggct gatgaggtta aaacaagcgt gggaacaacg agtatcagtg agcttttaga
    82381 gcaatttctt tttccgagat caagtcatgg cacgctgatt gctaaattat ctggtgggga
    82441 gaaaaaacgg ctctacttgc taaaactgct cattgaaaag ccaaatgttc ttctccttga
    82501 tgagccaaca aatgatttag acattgccac cttgacggtc ttagaaaact ttttagctaa
    82561 ctttgcagga ccagtcatta cggttagtca cgatcgttat ttcctagaca aagtagccac
    82621 taagatttta gcctttgaag aaggcgatat tcgtattttt tatgggagtt acagtgatta
    82681 ccttgatgaa aaagcttttg aaaaagaaac agttgaggct gacttagcta aagcaatagt
    82741 cactgaagaa gtgccacttc ctccgaaaga ggagaaaaag cgcatgtcct accttgaaaa
    82801 acaagaatgg gctcagatag aagataaaat agcaactatt gaggctaata ttgaagaaat
    82861 tgaaaaccaa atgttaactg tagttagtga ttatggccag ttagctcaat tacaaaagga
    82921 actcgatcag cgcaataatg atttattatt ggcgtatgaa cggtttgagt atttgagtgg
    82981 actagacata taagtatact atggtaatgt tatgtttata ataaacaagt gataatagaa
    83041 aagaagtgct cttgcactga gccccaaaag ttggctactc tatttgaagt aaaggattta
    83101 gttctgtatt gtagaggatt aagtcctttt cgttttattt tgatacgttt attgttgcaa
    83161 taatcgatga taatagtagg tagaacgagt caatttagcc gttcttaaaa gaaaatctaa
    83221 tcagaactcg tctttgtcca ttctttataa tggttttttg tcttcttctt tgcagagatg
    83281 cttctttcaa acggagttcc ctcggttttt ttagcgctaa atatctagct tatcttcata
    83341 gcttaatgtc atacaaaaac accccaaacg ttagattttt ttatctaacg tttggggtgt
    83401 agtttacatt atggcgcttc ttttctatta ttacgtgagg aaaaatcaag taaaaaagtt
    83461 aagtcaactg aaaaacttga cagaaatatt cagaaaattt ataatactaa ctattaacaa
    83521 catatcagct attaaagcat ttcaaacagc aggtgtttta tttggacctg ttaaggctgc
    83581 aaatgcaaga gaaagcagta tctggtttga aaatcttaca aagcaataac tatctctctt
    83641 agacatttga agaagcagac agtaaattcg ataatattgt gatgagaact tagcaacaat
    83701 ctgctgatgc aggtcaattt ttatttggta ctcatattgc agaatttcat aaagttgccc
    83761 aagatatctt gaaataagga atcacctgat gttttaaaca gatgatacta agtctatcat
    83821 atggtgataa tagtccattg aacatgtagt ttatgaatta aaaagaaggt ttaaggagat
    83881 aagaatgatt agagaaatca taacagatca ttttttatta cagcaaaaag cgcaagtcgc
    83941 caagaaagaa gatctatgga ttggccaaga tttacaagat accttagcct tttatagaca
    84001 ggaatgtctt gggatggctg ccaatatgat tggtgaacaa aagcgcattg ttattgtgag
    84061 tatgggattt attgatttgg ttatgtttaa tcctgtgatg gtttctaaaa agggaattta
    84121 ccaaactaaa gaatcttgtt tgtcgttatc tggctatcgc aaaacacagc ggtatgataa
    84181 gattactgtt gagtatttgg accataattg gcgaccaaag cgattaagct taacaggctt
    84241 aactgctcaa atatgccagc acgaattaga tcatctagaa ggtattttaa tttaacaaaa
    84301 aacctagagt aggacgcttt aggttttttt tggcaaagat gcctgattat ataagggtga
    84361 tcgattacta gtaaacgcta ggatttcctt tttttaataa aagcgtttag tccacctaag
    84421 cttatcaagg caaggattgt cataatagga gacattttat aattgtcccc tgttattgga
    84481 agagtggaac tagagatagt atttttgttt gttgttgttt cttttttgcc aaggctgtta
    84541 ttagtgtttt cagtagaagt caaaaggtct gaaatgatct cactagacac tatattgcca
    84601 tcacgatttc taaaaacctt actaatgata ctgtgtttac cagcactatt aacttttgtc
    84661 gaactttcaa ggtttgaagt tacataattt ttaacccctt ttatagtagc attaacagtt
    84721 ttacctgatg cttctaaatt tgtgatatat gtgatgaaag cttcagtatc tgtgttaata
    84781 gctccaatta atttagcttt tttaaatgct gaaaagccat caccaccacc ataaagaaag
    84841 tcgttgacaa caacggtgta agtagttgtt aagttaattt cttcaccatt gtctttataa
    84901 accttaacta tcttgaaggg ggtatcagag ttcttaggat cattatctgt ataagtgtat
    84961 gttaaacctg acatttgaag aaaataggtc tggttttcat cgtattgctg atttaggaca
    85021 tcgtaaatgt gttgaccagt catttgaatg acttgaagga tattaccaaa tggttgtaca
    85081 gcctgtgcag ctccccaggt gatggtccga tcatttttga caactaggtc acttcgaata
    85141 ccaccattat tggtcatagc aaagtcaaca gttggaaaag ttttcttagc aatagtaagc
    85201 tgagctgttg ttactaagtt accgacagga gattctttat caatattttc tgttttagaa
    85261 atagttgaag aattagttgc agttccgatt tttcgttcag taactgtttt aacaatatca
    85321 ttagcatgat ttattatagc tttgatatct gaattttctg ttttgatacc tggtgctaca
    85381 gcaataacat ttgctgatgg agttgtcata aaatcattgg tatcagtatc tagcgtacca
    85441 cggacatctg cataagcttt tccttgagag agagcttgaa cgatacgtgt tttaccgata
    85501 gttccattag tgtattgatg attatgtcct gcaaaaataa tatcaatgct atgttcggga
    85561 tagatttggt tcactttttc cataaccgta gccatttcat gatcaacaac accatcttta
    85621 cttgttgcag gaatatgagc taaaaccaca atagcatgaa catgttgttc ttgtagttct
    85681 ttagcatatt tggcaatggt ttcagctgca tctaaaaatt gatagtgttc atagttttgc
    85741 tttaaaacga gatttggaat ctctgtagtc acaacaccaa tgaagccaat cttaacgatt
    85801 ttgtcattaa tggctatgtc ttttatagca taaggtttcc aaccataggg gatatcattg
    85861 gtttttttat caataacatt agcaatgacg atggtttgat gcgaagcttc gtgctcatat
    85921 tgtttggtga tatcattaat tgttgattca ggatcaggcg cttgacctgt catgatacgg
    85981 ttaaattcat ctagtccttc gtcaaattca tgattaccaa gagtgccata ttcaaatttc
    86041 attttgttaa agacttttac agtaggctca tcttgtaaaa gtgcagagtt agcaggactt
    86101 gctccgacca tatctccagc ttgaacacgt atacttgttc cgtcttgatt tgcttgcttg
    86161 aagtctatct cagcgtcatc catataagca cctaattgag cagccgtccc agcatttggt
    86221 attttaccac ttggtgtgta agctgttccg gtattatcaa gagcgccgtg aaaatcattg
    86281 acgccaagga attgcacatc aacttgatct gcttgaacgt ctgtaactaa tagagtaaaa
    86341 ctagtcagga tactcaatac actacttttt aaaataaaat attttttcat aaaatctcct
    86401 aataagttaa cgtaatcgtt atagatcaag tttactatgt tttcgtttgt aattcaatgg
    86461 tatttttaaa aaagaacgca caaaaaatat atgaaagcga tttaaaaaca aacgataaag
    86521 atcgtttaca gttatatagt tagaaataaa gataaccgat cattgtagag atttaagcat
    86581 ttgttgagaa aatttgttat aataagcgta tgaaagaaag actagaacat tattctacca
    86641 gctctatata tagcggattt gaagtgtact tgccgttggt actacagaca ataacagatg
    86701 tgattattgc tgagaatatc aaaagcaaaa aagaaacagg gtttaaacta tacgaacact
    86761 ttacatcccg tattaaatcg gaagcttcga tgattgaaaa atgtcaacgc aaacaacttc
    86821 ctctaacaag caaatctgca ttaaaaatca ttaaagatag tatcggtatt cgcattattt
    86881 gtggttttat tgatgatatt tatcggatgg tagacttgct caaaagcatt cctggtatgt
    86941 cagttaacac tgaaaaagat tatattttaa atgccaagcc taatggctat agatcatatc
    87001 atctcatttt ggaattagaa acacattttc cagatatttt aggggaaaaa aaaggatgtt
    87061 attttataga agtgcagttg cgaaccattg ctcaagactc ttgggctagc ttggaacatc
    87121 aaatgaagta caaacatcaa gttgctaatg cagaaatgat tacaagagag ttaaagcgtt
    87181 gtgcagacga gttggcatct tgtgatgtta ccatgcaaac cattcgtcaa ttgattcaag
    87241 aaacgacgga ggaagagtaa ccatgaaaat tttgttagct gaagacgaat ggcagatgtc
    87301 taatgttttg acaactgcca tgactcatca aggttatgac gttgatgtgg tttttaacgg
    87361 tcaagaggca attgataaag ctaaagacaa tgcatatgat attatgattt tagatatcat
    87421 gat
//
LOCUS       DAUVUY010000002        65404 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.002, whole genome shotgun sequence.
ACCESSION   DAUVUY010000002 DAUVUY010000000
VERSION     DAUVUY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65404
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.002"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..609
                     /gene="prs"
                     /locus_tag="VOX58_000084"
     CDS             <1..609
                     /gene="prs"
                     /locus_tag="VOX58_000084"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HES5877363.1"
                     /translation="EVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRHGL
                     VGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGKKC
                     ILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVLDT
                     IYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            795..1550
                     /gene="recO"
                     /locus_tag="VOX58_000085"
     CDS             795..1550
                     /gene="recO"
                     /locus_tag="VOX58_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HES5877364.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            1653..2660
                     /gene="plsX"
                     /locus_tag="VOX58_000086"
     CDS             1653..2660
                     /gene="plsX"
                     /locus_tag="VOX58_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HES5877365.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMRKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            2653..2895
                     /locus_tag="VOX58_000087"
     CDS             2653..2895
                     /locus_tag="VOX58_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HES5877366.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHLAIPDEDVEDMVFMGDVLDYLVQRLT"
     gene            3046..3750
                     /locus_tag="VOX58_000088"
     CDS             3046..3750
                     /locus_tag="VOX58_000088"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HES5877367.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVIAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            3874..7599
                     /locus_tag="VOX58_000089"
     CDS             3874..7599
                     /locus_tag="VOX58_000089"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HES5877368.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKALRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIESLPGQFDQRAVSSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFETYKADDFTAYKVEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRAGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNDETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEARITSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNAYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKILSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFEAIQTQHKIT
                     AASAVKYGGVLESFALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKLEEVYPTEFEQTDVLEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEVAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQTLFASAVKYFTGK"
     gene            7760..9214
                     /locus_tag="VOX58_000090"
     CDS             7760..9214
                     /locus_tag="VOX58_000090"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HES5877369.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDRGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            9242..10264
                     /locus_tag="VOX58_000091"
     CDS             9242..10264
                     /locus_tag="VOX58_000091"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HES5877370.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELINGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            10432..10986
                     /gene="purN"
                     /locus_tag="VOX58_000092"
     CDS             10432..10986
                     /gene="purN"
                     /locus_tag="VOX58_000092"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HES5877371.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLTGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            11170..12717
                     /gene="purH"
                     /locus_tag="VOX58_000093"
     CDS             11170..12717
                     /gene="purH"
                     /locus_tag="VOX58_000093"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HES5877372.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVVLKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(12775..13899)
                     /locus_tag="VOX58_000094"
     CDS             complement(12775..13899)
                     /locus_tag="VOX58_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HES5877373.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFTVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            14152..15417
                     /gene="purD"
                     /locus_tag="VOX58_000095"
     CDS             14152..15417
                     /gene="purD"
                     /locus_tag="VOX58_000095"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HES5877374.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGVPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVRPVLEGMVAEGRPYLGVLYVGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFVENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIRE"
     gene            15695..16186
                     /gene="purE"
                     /locus_tag="VOX58_000096"
     CDS             15695..16186
                     /gene="purE"
                     /locus_tag="VOX58_000096"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HES5877375.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            16173..17246
                     /gene="purK"
                     /locus_tag="VOX58_000097"
     CDS             16173..17246
                     /gene="purK"
                     /locus_tag="VOX58_000097"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HES5877376.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGYKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENIDADGLDAVVSACQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLGGLDFTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTVFPVQENIHCNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFSDLPDEVEEF"
     gene            17248..18666
                     /locus_tag="VOX58_000098"
     CDS             17248..18666
                     /locus_tag="VOX58_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877377.1"
                     /translation="MYLKYIFKIKDRNLVSDYESLVRNLTINLQKLVIKDRTALIGDK
                     QLPITIYYRGENEKFPTGIVKVESDSVEGLELFDKTIKEIVGNESRFGKFTCIYDGIS
                     TYYSTEIYSELHEIERRTQEFLREAFFLSDKSGELKEYQATIKKMDSSDFSFSKLINE
                     IFKASDITDTFIDHVRACVEFGTVPNGDLLPKSFWDSLDDSLLDDKEQGLEINNLLNE
                     IRSTRNTIAHCKEFKKKDYINCIEQVKKLKQALDKIIASIESTDISKMSREVSRINNS
                     LQADNDYIKQKYFIEYDIFEKDILARTGLLEIDWENVSDDIEPLKIHRNFYESISEKT
                     DSEVIEEIKNLEDINFTDEKGNDLDDLTIIVPAQDEGFKRVFLGQREWYDIRIGKSKR
                     NRIKYIAGYEVAPESGVKYWAEVDKIVPSDNYVGYWKVIFKGEVKEYSEYKRLGNTWP
                     PQNSRYVSKKALDEAKYLSDVF"
     gene            18686..19978
                     /gene="purB"
                     /locus_tag="VOX58_000099"
     CDS             18686..19978
                     /gene="purB"
                     /locus_tag="VOX58_000099"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HES5877378.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEELDELFNPIYYTKRVDDIFKRLGI"
     gene            20109..21020
                     /gene="rgg4"
                     /locus_tag="VOX58_000100"
     CDS             20109..21020
                     /gene="rgg4"
                     /locus_tag="VOX58_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HES5877379.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     LILLELYLAYLDIEGMDGQYSDKIFYDSLLDNLSEQFEQFELDELFIVNKIIIDISSL
                     SLKNNRLDNLEKAIEMSQKIMAKIQDWNRMPILKLIEWKYFLIKQKDIIKAEQSFMKA
                     CLFAQMTADQYLENKLIQEWEKDVKSY"
     gene            21104..21199
                     /locus_tag="VOX58_000101"
     CDS             21104..21199
                     /locus_tag="VOX58_000101"
                     /inference="COORDINATES: protein motif:HMM:NF038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system DWW-type pheromone"
                     /protein_id="HES5877380.1"
                     /translation="MLKKYKYYFIFAALLSFKVVQELSAVDWWRL"
     gene            complement(21324..22412)
                     /locus_tag="VOX58_000102"
     CDS             complement(21324..22412)
                     /locus_tag="VOX58_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HES5877381.1"
                     /translation="MIEKYTKKDGTTAYRLRAYLGVDPITGKQVRTTRQGFKTEREAK
                     RAEVKLIDDFQRQGAWKSNDKTTFDDVAKLWFEQYRNTVKPSTFLVNQNYYKSTIKPH
                     IGKSQINKITVMACQNLINHLAQYSACASYISVINRIFKFAVHLGIIDNNPMDRTIRA
                     RCTYAHKEKMFYSKEELLIFLDIVKKEESLEMLLIYRLLAYGGLRIGEAVALYDTDFD
                     FINNTISVTKTLAQTKVGKVIQNPKTQKSSRTISMDQETMRLAKQYMRQSVTPLHGSF
                     RVFNLLTSTVRHRLKRTVERHHLKKINPHGFRHTHASLLFEAGVPAKLAQERLGHAKI
                     SITMDLYTHLSKNQTDNVAEKLADFIAI"
     gene            complement(22582..23103)
                     /locus_tag="VOX58_000103"
     CDS             complement(22582..23103)
                     /locus_tag="VOX58_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877382.1"
                     /translation="MKKVVYLFALSILIVCLGACSGGSKKDTTSKTPSSDTSKVSGDM
                     SEKEYFDTLISKIDKVTTDNYKSEEYLLYDYKTILRDPKKYFSLKVRINNLKIIQISD
                     KDKYTKVLANTPNGDLYMLFIETKRLETKLLETDNITINGRYLLSYEYTTTSGSENSV
                     PLIYIDGYLLLDK"
     gene            complement(23166..23492)
                     /locus_tag="VOX58_000104"
     CDS             complement(23166..23492)
                     /locus_tag="VOX58_000104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877383.1"
                     /translation="MYFDEFKEYVENNSRAHDYFFERALDYQNAKNARRTPAHKRWKE
                     QKVLKEVETMWTNVVKQLYDNIKLNGKFRKRFMHQEEIDEWVSFMNEHEILESFADSM
                     AEIEFE"
     gene            complement(23513..24070)
                     /locus_tag="VOX58_000105"
     CDS             complement(23513..24070)
                     /locus_tag="VOX58_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HES5877384.1"
                     /translation="MLEKVERLISEINRIHLVYSQDYFETGKVEKINLKHTFSKVPVQ
                     AILDYRLNLHESINDYLMKADVKDIPYVYRVKTSESILDKIERFSKRQDGYPVNSILN
                     DIFGARIILSSEDISQVMEQLDDWKDKFDLKNWYLRDKDSYTGIHVYFKNKSNHYYPW
                     ELQIWDEKDVDQNIESHKLFKRHFV"
     gene            complement(24072..24839)
                     /locus_tag="VOX58_000106"
     CDS             complement(24072..24839)
                     /locus_tag="VOX58_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5877385.1"
                     /translation="MRTNEEIITIIDELRKEKRLSISELARQVGMAKSGLSRYFNRTR
                     GFPLDRANDFAKVLGVTPEFLLGVQPINSEDKITTTEISNQPEIVSIYNQLEQPRQEK
                     VLNFANEQLEEQNKVVSMFDKKLEEEDYINDYVEGLVAAGNGTFQEDNLHMEVRLRAS
                     DVPEEYDTIAKVAGDSMEPLIQDNDLLFIKVSSQVDMNDIGIFQVNGKNFVKKLKRDY
                     DGAWYLQSLNNNYEEIYLSEDDNIRTIGEVVDIYRED"
     gene            25014..25232
                     /locus_tag="VOX58_000107"
     CDS             25014..25232
                     /locus_tag="VOX58_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5877386.1"
                     /translation="MGVDYLRVKAERIAKGYTQDYMAERMGWSDRARYAKRENGIVSF
                     DADELAKVVHILGFSKDQIGIFFTDSVH"
     gene            25263..25412
                     /locus_tag="VOX58_000108"
     CDS             25263..25412
                     /locus_tag="VOX58_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877387.1"
                     /translation="MPEDLIKQLEAGSEFLAKTCLHSKIIITVDGIRLVETKEFHPVS
                     GTLLD"
     gene            25487..25642
                     /locus_tag="VOX58_000109"
     CDS             25487..25642
                     /locus_tag="VOX58_000109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877388.1"
                     /translation="MVTKFSEEIKIVRTKSSVYVNSVLDVADKKDPASVAAVAELLKA
                     YKSLTEL"
     gene            complement(25987..26196)
                     /locus_tag="VOX58_000110"
     CDS             complement(25987..26196)
                     /locus_tag="VOX58_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877389.1"
                     /translation="MNPNYFHVQFKLGEKVSYNTPSAEGREVIPIKGAEVTKMIFADG
                     NELLSVIYNETVDVYASFPIVLEYH"
     gene            26271..26459
                     /locus_tag="VOX58_000111"
     CDS             26271..26459
                     /locus_tag="VOX58_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877390.1"
                     /translation="MRPKRYPYQKNKLIIQSKNAKTKIELDSGKITISAEEIKVGDLA
                     NEISKVQLTKEKVKLPMS"
     gene            26563..26820
                     /locus_tag="VOX58_000112"
     CDS             26563..26820
                     /locus_tag="VOX58_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877391.1"
                     /translation="MQSQLTYDLLKKQIAEELFDEFKKLIQEKDLANQWVNQTTLVTE
                     YGYSWQTIKRMESYGLKSFKNGKDKMYCLADINDIKHLMKQ"
     gene            26838..27032
                     /locus_tag="VOX58_000113"
     CDS             26838..27032
                     /locus_tag="VOX58_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877392.1"
                     /translation="MNKLEILLLTSTIILAIIARVQHEVIKKHNSPENKRRIFREVAL
                     ENSKGWSDKRSRGEVEKCLL"
     gene            27077..27838
                     /locus_tag="VOX58_000114"
     CDS             27077..27838
                     /locus_tag="VOX58_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HES5877393.1"
                     /translation="MHIDEVRNNKFYQFPQWLLEEPYSNLSYRAMLMYMLLFDRRGLS
                     EENQWFDDNGKIFMYFTNEQFMKALKCSEPSVLKSKKELHQAGLLDEVQQGVNKPNRL
                     YINGTKNFLGQELKTFKHGTKNFLGQDLKSFKGINTNNINTNISNYYDDKGTSFLEEI
                     ENLWGRQFNGYEVQQINRFLLEEHISNDLLKKAIEISLANGARNMNYVSVVINNWIDS
                     GITTVEQVDAAQELRLKQKSKTNSKDEEVSEYDTW"
     gene            27825..28607
                     /locus_tag="VOX58_000115"
     CDS             27825..28607
                     /locus_tag="VOX58_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HES5877394.1"
                     /translation="MILGDEDALAKIAISYQQNTKKEDVVCEKHNCRYITVLKTGLTV
                     CPDCHREELENQNTLHVQKQYERELENKRLYYLKKLSIMDNELENASFDNFRADTAKH
                     KEVLSWAKTMANDWFIGGKGNIIMTGKAGRGKSHLAYSIIRGLSDKTKKLGLLVNVTD
                     LLSEIKRDFSKEAFWMDKLKDVDYLVLDDLGAEKVSDWSTSIIYSLLNKRTNTIITTN
                     LTPAEIRKIYGERIASRIRKGCDKRHIMEFDGMEDERMKLWN"
     gene            28598..28834
                     /locus_tag="VOX58_000116"
     CDS             28598..28834
                     /locus_tag="VOX58_000116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877395.1"
                     /translation="MELKKRNKFIENTANLILQMADENKKENIANLPDYIRGMFDLCI
                     AFMLEEDKEGTGYDSVIVMIAEESERSKNERDAN"
     gene            28965..29312
                     /locus_tag="VOX58_000117"
     CDS             28965..29312
                     /locus_tag="VOX58_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877396.1"
                     /translation="MVVPLDELEKRIFCYLPRGSNRKVNSKEICRTFDIKDPKTLRDI
                     IHGMVMKGYLIGSSRRKGGGYYLIDNDDERAEAISSIESQVRKEQKRINILKNGNLNE
                     FVEIANEVRNGRN"
     gene            29299..29553
                     /locus_tag="VOX58_000118"
     CDS             29299..29553
                     /locus_tag="VOX58_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877397.1"
                     /translation="MVEIRINGETITFDSNFRDAFIFTIDCLRGSEEPTLKQTYQEFK
                     DYTDEDLMDYIETEFDVKPELIPNRRIDSKWIFKQHILDD"
     gene            29556..29729
                     /locus_tag="VOX58_000119"
     CDS             29556..29729
                     /locus_tag="VOX58_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877398.1"
                     /translation="MGKSDFKFSEDWESNYFEVQALLGQEIDKLQNRVVQLANENRRL
                     KAENWQLKHRKRK"
     gene            29731..30060
                     /locus_tag="VOX58_000120"
     CDS             29731..30060
                     /locus_tag="VOX58_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877399.1"
                     /translation="MNIKEKIVVLRKTKDGSFLKSFKNRDAVLAYNKEFTNIIQAASF
                     LPEEAYNMQKDKIDNLAETFGCDVVVVEASYDLKFIDGEDVPELTKEQKIESMVNGMF
                     EQVFGGE"
     gene            30063..31022
                     /locus_tag="VOX58_000121"
     CDS             30063..31022
                     /locus_tag="VOX58_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HES5877400.1"
                     /translation="MANELSERQITSGVNKRIEEKQNENFIVPPNYSLGNALSNAYYE
                     LKNSSSGNLLSQCTDESIYISLLDMVAQGLSPAKKQCYFIKYGDKVQLRRSYFGTMKV
                     VKELNEVKDIWAGVIFEGDVFKSEIVNGRRRFVSHESDWENQDNPIKGAYCIIKDINN
                     EEYLTIMTKKQIDKAWSKAKTKNVQIDFPDQMAMRTVINRAAKLFINTSNDSSYFVEA
                     LNRTTENEYDNDRQVKDVTPQETNNLDDLIGHQNEKIDAPSNLKDVTEDLHSEPEKTL
                     TDENKAVLEDTSYPADEIPDFDQETGEIKASEGNLFDNLGDLI"
     gene            31019..31219
                     /locus_tag="VOX58_000122"
     CDS             31019..31219
                     /locus_tag="VOX58_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877401.1"
                     /translation="MIKLVKIEGYYINPEYVVGVWERSALNFDDLDEKVVVVQFVGDR
                     EREEFVFYDTPIDEVIKRLLDD"
     gene            31212..32009
                     /locus_tag="VOX58_000123"
     CDS             31212..32009
                     /locus_tag="VOX58_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HES5877402.1"
                     /translation="MTSLDLLGKDYYSRESAIRYWSISQYKRFRECEARALAELRGDW
                     TDTRDNTALLVGNYVHSYFESKAAYSNFVEANKTAMLSTRGATKGQLKKDYAVANQMI
                     EALKSDSNFTAIYQGEKEAAITGLIGGVEFKGKIDCLNVDRGYFVDIKTTKGPIDDTI
                     WNGEERVRWFEAYGYILQMAAYKTMLEAKYNKPFEPIIYAVTKETPPDTRAIRIQNVD
                     AMQMELDSLAQSIKRLDDVKKGLEKPKPCGKCEYCRQNKLSVRVEIF"
     gene            32187..32528
                     /locus_tag="VOX58_000124"
     CDS             32187..32528
                     /locus_tag="VOX58_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877403.1"
                     /translation="MEQIKITGTGTALILDRVNRIFAISGGLTMQWDFISDFKKIDDE
                     PSLDEDGELFEVAYDLVLEAKPKTKINLTSSYFAKEHKKDTDEIIKVFSFIEDNKKNI
                     FETLGIRGVLE"
     gene            32525..33037
                     /locus_tag="VOX58_000125"
     CDS             32525..33037
                     /locus_tag="VOX58_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877404.1"
                     /translation="MSNLVLSLDISTSGTGWALFKGSDLIQSGVLKHKSDSYFERGRY
                     MASQLRLIQSRALKKYDCSFSTIAVEKNSVMGPNQQSMLKIGIVTGIILGRLIADNVV
                     FVNVSTWRKHWKFSYKDRSKKSMKLQSKTKVEQEFNKSVKDDEADAILIGSYYVNQGY
                     LDGLETHDYY"
     gene            33024..33218
                     /locus_tag="VOX58_000126"
     CDS             33024..33218
                     /locus_tag="VOX58_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877405.1"
                     /translation="MTTTKKRVVRVYNKGITATYMVYDKNLFKEHEFVTKVEARQFIR
                     RLELANDKRVTEYYLREVKE"
     gene            33215..33631
                     /locus_tag="VOX58_000127"
     CDS             33215..33631
                     /locus_tag="VOX58_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877406.1"
                     /translation="MIPKFRAFNKKTKKMYSIDGFKASERKIYRCSFADDEFRSGRLE
                     TFHFVEDNLDDYILMQSTGLKDKNGVEIFDGDVVNFMVPNQMISGTYQIRQAKSGEWR
                     LDNRVQGRPLYISGSYHCEVVGNVWEDGDLLESVEE"
     gene            33824..34000
                     /locus_tag="VOX58_000128"
     CDS             33824..34000
                     /locus_tag="VOX58_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877407.1"
                     /translation="MTKEELIEEIEGEINDIDLLIDDYQYEIDELQRDIDELDTRKDI
                     LVRKLQNLLESVEE"
     gene            33997..34266
                     /locus_tag="VOX58_000129"
     CDS             33997..34266
                     /locus_tag="VOX58_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877408.1"
                     /translation="MTEEQMIDCLLYELAKKDKAIEKKDIIIAVLKVMLCLVFVFGIA
                     LQNHHKPQITGLQSQLRRTQKQLKRASEQNQQQTKRIAELTGNGG"
     gene            34358..34567
                     /locus_tag="VOX58_000130"
     CDS             34358..34567
                     /locus_tag="VOX58_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877409.1"
                     /translation="MTEQEIKNLLNDSRKLVSEAINKCYLATSKIPLSVDNSLIFSAY
                     DYIIHDIGDVDKIAKEISKAGENEH"
     gene            34843..35451
                     /locus_tag="VOX58_000131"
     CDS             34843..35451
                     /locus_tag="VOX58_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013794414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HES5877410.1"
                     /translation="MNIEEVLYLPVKREGLSKGPDEFDCKHRYRTLDGDEVASSFKAK
                     SKEELLDQPKPVVPQCAIDYYEQYKELLSDFEEWFGDFYDSYFLKEFPKGIELAEWLH
                     DNDNKTNLQHELALATLIVNGPDAVTVEKEKLYTVEIPNPNIIGNEHTVLMKNGFGQI
                     VMFRVYGDDWRTDKGYQNTESEIRKYFDWAWREGFQKEVTDD"
     gene            35444..35617
                     /locus_tag="VOX58_000132"
     CDS             35444..35617
                     /locus_tag="VOX58_000132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877411.1"
                     /translation="MTEELGVLLDKEGIEYVKMVFDDGFVWTVRNPLNAALLKRQEAE
                     QFPQFTWVPLTDL"
     gene            36084..36521
                     /locus_tag="VOX58_000133"
     CDS             36084..36521
                     /locus_tag="VOX58_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HES5877412.1"
                     /translation="MGNIPTTKANNFLEELKTIPHLIETLERDANLMSRSLVKSPQWS
                     DMKVSGGVKQSQEDKNIKMLHMVSYYSDQIERLKDRRQEMANLIVQSMGICESHVLLT
                     TYLDCDGDYERARERLNIGNRNKYFMFVRRGKESLELILKNTN"
     gene            37133..37609
                     /locus_tag="VOX58_000134"
     CDS             37133..37609
                     /locus_tag="VOX58_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HES5877413.1"
                     /translation="MGLTTKQELFVQGIVSGLSQRQAYRRAYKSENMADDTVDKKASL
                     LFNKGEIRGRYRELLKQFSNMSLWSREQAFNEYEWLKNKARQDIENEGVRQANSNAFL
                     SALEGMNNIAFKELELEDKKLVKEIELLQIKLDAEKGSKPDTSLMRALLDAVEGDD"
     gene            37647..38897
                     /locus_tag="VOX58_000135"
     CDS             37647..38897
                     /locus_tag="VOX58_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HES5877414.1"
                     /translation="MRRPFNYELEVNEGTPRSGKTTAGHFRYARYLIESEDENHLVTA
                     YNQEQAYRLFIDGDGTGLMHIFDGNCEIKHDERGDHLLIMTPKGNKRVYYKGGGKVNS
                     VGAITGMSLGSVVFCEINLLHMDFIQECFRRTWAAKLRYHLADLNPPAPQHPVIKDVF
                     DVQNTRWTHWTMDDNPILTAERKQSIINNLRKNPYLYKRDVLGQRVMPQGVIYGLFDM
                     EKNVLDTLIGEPVEMYFCADGGQSDATSMSCNIITRVRDNGKISFRLNRVAHYYHSGA
                     DTGQVKAMSTYALELKVFMDWCVKKYQMRYTEVFVDPACKSLREELHKLGVFTLGAPN
                     NSKDVSSKAKGIEVGIERGQNIISDGAFYLVNHSEEEYDHYHFLKEIGLYSRDDNGKP
                     IDKDNHAMDEFRYSVNVFVHRYYN"
     gene            38911..40413
                     /locus_tag="VOX58_000136"
     CDS             38911..40413
                     /locus_tag="VOX58_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HES5877415.1"
                     /translation="MGVIQKIKNLFTRSKYVMTTQSLTNITDHPKIAISKLEYDRITT
                     NLKYYKSDWDSVLYLNTDGETKKRNLNHLPIARTAAKKIASLVFNEQAEIKVDDDAAN
                     KFISETLKNDRFNKNFERYLESCLALGGLAMRPYVDGDKVRVAFVQAPVFLPLQSNTQ
                     DVSSAAIVIKSVKIINGKEVYYTLIEFHEWRSSDDYVISNELYRSDDKTKVGSRVPLS
                     EVYEDLKDEAKVTDVTRPIFTYLKTPGMNNKDINSPLGLSIFDNAKTTIDFINTTYDE
                     FMWEVKMGQRRVAVPESMTALKIHTADGDVIPRPRFESDQNVYIRMGGRDLDSTAIQD
                     LTTPIRADDYIKAINEGLSLFEMQIGVSAGLFSFDGKSMKTATEIVSENSDTYQMRNS
                     IVALVEQSLKELVISIFEIAKAYDLYQGESPNMDNISINLDDGVFTDRDAELDYWIKV
                     VNAGFGTREMAIQKVLNVTEEKAQEIAAEINTGIVDKINQQRTDTHLYGE"
     gene            40418..41914
                     /locus_tag="VOX58_000137"
     CDS             40418..41914
                     /locus_tag="VOX58_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HES5877416.1"
                     /translation="MKKKPIKLNDEQLLLEASQLSDMYHQLTLDLFDQVIERIKARGS
                     ASLADNPYLWQANKLHDVGLLNADNIKLIAKYSGIAEAQLRYIIKNEGFKIYKNTSEQ
                     LEEALGRESGVNSTIQDDLSNYARQAIDDVHNLINTTLPISVIGAYQGIIQDAVAGVV
                     TGLKTPDQAINQTVIKWFKKGFYGFTDKAGRKWRADSYARTVINTTTWRVFNEAKEAP
                     AREFGVDTFYYSKKATAREMCAPLQHQIVTTGEAREEEGIKILALSDYRHGEPDGCLG
                     INCKHTKTPFVVGVNSKPELPEHLKNITPAQAKANANAQAKQRAIERSIRKSKELLHV
                     AKKLGDRDLISQYQSDVSSKRDALNYLINNNAFLHRNQVREKRYNNSYTKTQSEVEVR
                     KEKAKLDKRRDVESAIIGVETSEGIPLKITKHLAERAVLRNIAPIDIVDSIREPLKIA
                     PIKYDNLDRPSQKYIGKRVSTVINPIDGNIVTVYATSTRIRKKYGGNR"
     gene            41911..42138
                     /locus_tag="VOX58_000138"
     CDS             41911..42138
                     /locus_tag="VOX58_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877417.1"
                     /translation="MKLKNILNDSQIDFVKNELPGLPVDIEVTSEKYDVFCEGIETYY
                     QTESFDEKYNITAKGKLAESIIDLLTDKGYW"
     gene            42212..42481
                     /locus_tag="VOX58_000139"
     CDS             42212..42481
                     /locus_tag="VOX58_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877418.1"
                     /translation="MNKRIKKKRKLETAIVLLIAENTMQAEAIKNQDRQIAELRAIVQ
                     QNAQATNNELATVTGVCFDNQAAIVDIAIDVNYIKQNYKRKWGKK"
     gene            42483..42740
                     /locus_tag="VOX58_000140"
     CDS             42483..42740
                     /locus_tag="VOX58_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877419.1"
                     /translation="MEYTEKAKELASQEFTCLSGREIKPEDCFVVWFCKTLQNWKALV
                     STNQIKTGEKCGDYAEITHNGDKKETYVDVYAKVSNQAFAD"
     gene            42978..43586
                     /locus_tag="VOX58_000141"
     CDS             42978..43586
                     /locus_tag="VOX58_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877420.1"
                     /translation="MENVMNENVDQETTGLENNQQEDKAFTQDDLNRVGTKEHSKGYN
                     KAVKDLGFDDVESAKDALKAFQEWKESQKSESEKQTEILNAKDKELEEARAINKALNA
                     KLAAMSLGVKAESIDDVIALSERLVTDETSIEDAIKTVLGKYPHFGQTKDKAPKITVA
                     GNPSADNGQGSVSKEDFAKMSYQEKLDLKLKNKNLYDQLKGN"
     gene            43589..44425
                     /locus_tag="VOX58_000142"
     CDS             43589..44425
                     /locus_tag="VOX58_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HES5877421.1"
                     /translation="MAVGTTKMAQMLDPEVLADMIDAEVGKAIRFAPLAEVDTTLEGQ
                     PGTTLTVPKWDYIGDAEDVAEGEAIPMTQLGFKKTTMKIKKAGKGVEITDEAILSGYG
                     DPVGQAAKQIVEAIDHKVDADVLTALSGSTQTVSAPASVEGVSQALDIFNDEDDAETV
                     IVMNPADASTLRLNAAKEWLGATEVGANRIVSGVYGEVLGVQIVRSRKCPKGTAYMVR
                     KGALRIMLKRNTMVETDRDITKAINQIVANKHYGVYLYKAEKTVKITITEAKKKEKNF
                     EM"
     gene            44435..44704
                     /locus_tag="VOX58_000143"
     CDS             44435..44704
                     /locus_tag="VOX58_000143"
                     /inference="COORDINATES: protein motif:HMM:NF024351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877422.1"
                     /translation="MNKYKAMVGFTSKAEGVSCSKGQVVELDEAIANQINDKSFEKYA
                     DYAPFLELVEQKKQTVADIKKALEEKGIDYPSKATKEELLALLAE"
     gene            44736..45137
                     /locus_tag="VOX58_000144"
     CDS             44736..45137
                     /locus_tag="VOX58_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013794407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877423.1"
                     /translation="MTFLTQKEFVDLGFDEVEDFEKMEKRAGHIINLYCRNRYDYKDF
                     KNEIALVQKAVKRAIAYQIAYLNDSGVMTAEDKQSFAGISLGRTSISYTVGHGQSSQQ
                     KTLADRFNLCLDAENELLAVGLGYTGISYDR"
     gene            45127..45459
                     /locus_tag="VOX58_000145"
     CDS             45127..45459
                     /locus_tag="VOX58_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HES5877424.1"
                     /translation="MIDKRLLIDKLQVKLVKEKGDYGGFVYDEPFIVSPVRFDRNLST
                     AGKDNARQETKPSVIFIYPKYCKVAADRTWVDAAVLDGDTEYIVDKVIPVYHPLTNKV
                     FCYEVEVI"
     gene            45459..45815
                     /locus_tag="VOX58_000146"
     CDS             45459..45815
                     /locus_tag="VOX58_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HES5877425.1"
                     /translation="MAKIVVELGGIKRKVSPQALARGKLVMNNQVMMSMNPYVPYRDG
                     ALRGSSRANSMGVMWSGPHARAQFYGGAYNKYKSFKFKKYTTPGTGKRWDKRALANAT
                     IVKDWEKSLLRGMGFK"
     gene            45812..46210
                     /locus_tag="VOX58_000147"
     CDS             45812..46210
                     /locus_tag="VOX58_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HES5877426.1"
                     /translation="MTNDFATVLRQFIEGLDLGLKPRLDYLTRQEDLAIYPMPGGKVN
                     SEYMDGTREISLPFEIAIKTKNQELANNVMWTINSALSNFDLKLPSLNNSYTFTSLDV
                     EKPFLNDLSDQGFYIYVLDITAHLEIERKN"
     gene            46210..46698
                     /locus_tag="VOX58_000148"
     CDS             46210..46698
                     /locus_tag="VOX58_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HES5877427.1"
                     /translation="MARQKNALRGHFIAPYKTGISETEVTDETVMELAKWIKDISDDT
                     DEKTEDEAYYDGDGTEETTVVGVKGAYTFEGTYDPEDKAQAHIASLKYKLGDERKVWH
                     LIVAADGKKQWLGVATVTEIIAGSGAAADFEAFGCKVTYNTLPKESSEMTLKKKEKNF
                     EM"
     gene            46741..47175
                     /locus_tag="VOX58_000149"
     CDS             46741..47175
                     /locus_tag="VOX58_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877428.1"
                     /translation="MSMINIDLKRTGFPVKIGVVELWFDTSQERLLEFFDIETEVSRR
                     LNEYEKQIIEANLNKRIEDEGVTKEVAKGALDLETKYLEINYDLLFGDGTFNKLYETY
                     PDKEALESTLEVVSREIEVKLKELAIEREKIVKQKMKKYKKE"
     gene            47179..47760
                     /locus_tag="VOX58_000150"
     CDS             47179..47760
                     /locus_tag="VOX58_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage Gp15 family protein"
                     /protein_id="HES5877429.1"
                     /translation="MKLNDPLVVSFEFRGETYPIDLSFNKVLDVFDVIDDDFLNETEK
                     CFLCLDILLDRTELPFTYAVDLWIYIKKNYIDTERPEKPQLDIKGNPMPIVKEKEDNK
                     KVIDFSLDAEFIYASFRQAYQINLLKVQNKLSWIEFKALLNALPEDTIMQRIIAIRQW
                     EDDGKGDKDYRDNMRKLKAKYSLDDGEEGDDGS"
     gene            47750..51010
                     /locus_tag="VOX58_000151"
     CDS             47750..51010
                     /locus_tag="VOX58_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HES5877430.1"
                     /translation="MAADGKVTILVDVDGKQVKVLNNELDKVAKHGDKGSSSLKKFAV
                     GAGVFKLASAAVDLVSQSLGKAITRFDTLEKYPRVMKAMGHSAEDVARSTDKLANGID
                     GLPTTLDEVVGTAQRLTSITKDINKSTNLTLALNNAFLASGASSEAASRGLEQYAQML
                     SAGKVDMQAWKTLQETMPYALQQTAEAFGFAGASAQKDFYEALKNGQITFDQFSNKLI
                     ELNDGVGGFAELAKENSKGIETSFNNIKNAIAKGVANSIKALDDLSKAATGKSIADHF
                     DSLKVVINASFSAINASIKASTPLFKLLFSVIGAGISVVKALSPALVGVASGLAAMRA
                     VNETITMIKALNRAWVMASASMSIGATTIKTVTAVQAVSTTMTKADMVARLSQLGVLK
                     ASTVIYGVMTGAISLSTAATIASTAAVTALKAALVALTGPVGWVVGAIGALVAVGVSL
                     WSWLTKESDETKKLKKEQEGLVESNKQLRDSVREGVQERKKSLESVKESTAAHQKLAD
                     EIIKLAAKENKTAGEKQNLKNKIDQLNGSIDGLNLAYDKNSNSLSHNADQIKSRISAM
                     EAESTWQTAQQNLLNIEQKRSEVSKKLAENAELRKKWNEEANVSDSVRKEKIAELTEE
                     EGKLKNMQTQLQEEYNKTSATQQAAADAMAAAEESGSARQVLAYENMSEAQRTAIDNM
                     RTKYSELLETTTSIFDAIEQKTALSVEQMNANLEQNRLATEQWATNLEILAQRGVDQG
                     ILEQLRRMGPEGATQTQVFVDATDAELAPLQENFRAATETAKNAMGSVLDSAGVEMPE
                     KVKGMVTNVSTGLQAELQAANFAQLGQEIPNGVSQGISQGTGKASDASTKMGQEVKRS
                     FQGELGIHSPSRVFTEYGGHITDGLSNGVTNGTSKVMQTMQSMAQQMSQKGQQIVNDM
                     RTKSNQITDAFSTMSGPMHSHGVNAMQGLANGIYAGSGVALAAAQSIAARITATIQSA
                     LDIHSPSRVMRDEVGRFIPQGIAVGIDADRKVIDSSMQKLKESMTINATPELASGFSG
                     GVAGVANQTTNNSNNSFTLNVKVDESDGNSREKYHRLFREFSWYIQQQQGRLGDVK"
     gene            51007..51723
                     /locus_tag="VOX58_000152"
     CDS             51007..51723
                     /locus_tag="VOX58_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HES5877431.1"
                     /translation="MTAFIKFDGKKSSDFGLRIINDVEHDSTFYDVDQVKVPGRDGVV
                     LKNNQRLEAVERSYPLRLYSKRRLTEAETDISNWLNVTGWKDLELSWEPDYVYKATHI
                     TPFSIKEVLRNFGRLKVNFLIHPVKYLKTGKQEVSLVNGGTLQNPGNVQSKPILKIKG
                     TGNGVLTINGFETGLENVQGELLIDMERRLVYKDVLSAWDNIVRTERHRMPLFDIGQN
                     KISWTGNFTITAVPNWGVKV"
     gene            51720..53867
                     /locus_tag="VOX58_000153"
     CDS             51720..53867
                     /locus_tag="VOX58_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HES5877432.1"
                     /translation="MIPVLYEARETKFRTFGLGEIADAYEVKATRERNGNYSLYIKYP
                     LDGIFASTFKEEMKIKSDAGRRTKWQTFEINRVLRNSKDNIEIFARHISMRTQDIALK
                     PFVNGASVGAESALEIWKENLVGDDTFDVKSDILTLGSFNWEIDKVGNARSALGGVAG
                     SILDVYGGEYEFDNRTIILHKQMGRKAPTVLEYGRNIVSVEEERLLDGNYTSIYPFVR
                     YTPQSKPQEEASGKPHVGEQEQPEEQLVTLPEFILDGQYLDLYAQRRIQMVDLSSHFN
                     DDKGKKEPTVEEIRKLAQKYLKDNNVGAPKVSIEVDYIDLSQTLDYQDFRVMEEVELC
                     DIVPLYYPKFGITTESEKVVEIVYDVYTDSNHTIKLGTIGQSISKSLTGGVSERINAL
                     ENNQKVITNNQKQFELNLPKYLNDLNGNRVWYEKPDDNIEHKIGDYWFEKNGKYQRTW
                     IWDGNQWVKVLDTEDLNPNQRAFDEAMAEIEKAKKAQEEINQRTDKELEEFRDTLKNL
                     ALPEEAIKKITEAIKVDDIPSIKQSFDDLKNKVSETSEESRLTAEILGNNGKTRYNKN
                     LLVGDPNRTKTYDEDFIEVEANDGGFKRGETYTISFSQTCDLLKKVAITLTQVNNKGL
                     KLVLTPTKAKMEPQTFNLTKDKEVISVYPLSYTAVLTGEWYKSKQVDLNASEVQNLAL
                     EMAYRDVADARGATIIGKQSDKPKIILDGRRDR"
     gene            53864..55222
                     /locus_tag="VOX58_000154"
     CDS             53864..55222
                     /locus_tag="VOX58_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877433.1"
                     /translation="MTLEERIPIKVLFDRKDAAEWQRLNPVIDDGELVVELDTHRLKV
                     GDGKLNYNDLPYYEGPRGESITKVQLSENGDLSVWIGEKETKLGNIEGQKGDKGTSIT
                     DITKDGETMTIKLSDDTQKTFSIPNGQKGDRGKGVESARVDGSGHLRLKIEEEQEKDL
                     GNVKGDKGDSITITDQQRVSEGVQLTFSDKTKVVIPKGEKGDTGDVNGINLDDYVKKS
                     DLKTVGAPDVKAINDFLGLSQKVFTSKYSYSDSLLKSYSSPSYSASWYVNESTKDVNV
                     KDKVLITISNTTTRMDNYLEVSVTYVGSNYVTATSTGKLLTTPGEVNVLTKEQIAAAY
                     AKKEHKHEIDDIDGLQGTLNRKADVIHYHRIDDIAGLQQAFNDRPTMERISQTLATAL
                     EDVVRQGQLSGYVRSADIQNQLNNVGKVKDEVTGQYLSVRVVDNGQVPRDTSGMIIFE
                     RA"
     gene            55222..56067
                     /locus_tag="VOX58_000155"
     CDS             55222..56067
                     /locus_tag="VOX58_000155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877434.1"
                     /translation="MMDLSDKYLMSIVDGRISSFTTGDSLVVNNYYIDYNDTYKFDNE
                     DLTFYYATSSHEAIAYKANRNNYKVLNPQNKAVNSLYSAITVNGQQIAKIVSNGKIIW
                     QKDKMTFLDLSVGRRVQVSFNGRTITIVFGATPNSKDFEQFFAASNKDLLLTIDNQSY
                     DVVAAEYVKFASLVKFTVTQFSSYKNTTATNVTVAVKTDDTAQSQVLLFDGRPTTTFT
                     KLDLYKTYIFERPGYPPETREATALDFGYLKLGDGHLLINKSDTGLYIKSYGTAFDVK
                     IYGKN"
     gene            56078..57982
                     /locus_tag="VOX58_000156"
     CDS             56078..57982
                     /locus_tag="VOX58_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HES5877435.1"
                     /translation="MSRDPTLILDESNLVIGKDGRVHYTFTASQDNQKVRLKSNCLGT
                     AHFNQLMIERGDQATDYVAPVVVEGSGQSTGVFKSLEEMLSGLQSINLELTDTENSKL
                     WSKIKLTTNGMLREYHRDNITTEIVESADGIATRISEETAGKLALINDTVSGIRREYQ
                     DADRKLSSSYQAGIDGLKATMASDKLGLQAEIKASAQGLSQKYDDELRKLSAKITTTS
                     SGTTEAYENKLDGLRAEFTRSNQGLRVELESQISGLRATQQSTASQISQEIRDRTGAV
                     SRVQQSLESYQRRLQSAEDNYSSLQQTVNGLQSDVGSPTGKIQSRLTQLAGQIEQRVT
                     RDGVMSIISGAGDSIKLAIQKAGGIDAKMSASEIISAINLNGYGVRISGERIALDGNT
                     TVNGTFTTKIAEAIKIRADQIIAGTIDAAKIRVINLNASSIVGLDANFIKAKIEHTIT
                     SLLEGKVIRARNGAMMIDLNSAKMDFNSNATINFNNKDNALVRKDGTHTAFVHFSNAT
                     PKNYTGSALYASIGITSSGDGINSASSGRFCGARFFRYARGYEHTALVDQAEIYGDDI
                     VFSDDFNIDRGFKMRPSLMPKMVDLNKMYQAILALGRCWLHANNTAWTFNSDTTSAII
                     REYNSYINGL"
     gene            57991..58419
                     /locus_tag="VOX58_000157"
     CDS             57991..58419
                     /locus_tag="VOX58_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HES5877436.1"
                     /translation="MDLTVQNKDLNTLYSVLDKIKVTNMRVNRGRAKLLAKVEAKLRE
                     YAKDEVDIIDQYAAKNDKGKWLVDDKGNAKLADVTKVTELNDCLDELANEPIVIKGHE
                     YSKRFIDFLEYLAEAEDEFTGAEIVLIDNILEQYEESKGE"
     gene            58422..59036
                     /locus_tag="VOX58_000158"
     CDS             58422..59036
                     /locus_tag="VOX58_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HES5877437.1"
                     /translation="MARNWKVTGKYPQFDSTGAVASTHVIIAAEDGAVIPQLVKKDLT
                     GTNDKDIITAVLEEFKKSEYVEIAMGEAVKKVDDLEKISQETAKTAKTAQSAAGLAKV
                     SAERTQKMINLQTIHVLTSSDKVEPDIYKGMLELIEPAKKGEYQAYDVFTIVDESHEE
                     QAGEGNLVFVHVNEPFTYDKQTLKKLEEEDKVTVIKYADLVKQD"
     gene            59047..59376
                     /locus_tag="VOX58_000159"
     CDS             59047..59376
                     /locus_tag="VOX58_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877438.1"
                     /translation="MHEILIQIKELAGAVSALALIGGAMIWIYKKLVIEPDSRLAERL
                     QTENNKLLTDTVNPLTDAIKDLNYNLNTATKERAEMRKDIEVHEGRLDAHDIRLTVLE
                     TKEIKEK"
     gene            59378..59710
                     /locus_tag="VOX58_000160"
     CDS             59378..59710
                     /locus_tag="VOX58_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HES5877439.1"
                     /translation="MQEITNIITGSSLSILTILAGIVVKLVKDYLLKKGGEKAVKIAE
                     IVARNAVEAVEQIAYDKDIKGIEKLTEAKVAVRDELSKHNVYLSDKQMEVFIEAAVKR
                     MNDNWKGQ"
     gene            59712..60929
                     /locus_tag="VOX58_000161"
     CDS             59712..60929
                     /locus_tag="VOX58_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HES5877440.1"
                     /translation="MTVDTEKAIAWMGSKEGRVSYSMTYRNGPNSYDCSSSVCSALIY
                     AGASNPGWLLNTEYMHDWLVKNGYTLIAENDGWDSQRGDIFIWGQRGQSAGAGGHTGM
                     FVDADNIIHCNYASNNITIDNYNQTAAASGWMYSYAYRYVGATDTFVGSKRIDELAQE
                     VLAGKHGSGEQRKLSLGSNYDAVQTKVNEMLKQPQVAEQSPAVKQDGDLLFNGVVLKR
                     AILDKILAKCKEHDILPSYAITVLHFEGLWGQSAVGRSDNNWGGMTWPGKGERPSGIT
                     VTQGTSRPLNEGGHYMHYESVDDFLTDWFYLLRAGGSYKVSGAKTFSEAVKGMFIIGG
                     AKYDYAASGFDSYIAGMASRLKAIEQENGPLSKYNQQTDINVGQSDKIDVVIDSLEIT
                     INGVNYTATKKPI"
     gene            61288..61800
                     /locus_tag="VOX58_000162"
     CDS             61288..61800
                     /locus_tag="VOX58_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877441.1"
                     /translation="MIVELKEIVNDYELNFCGKRCKVETNFKHLPEFMILFDIRDLYH
                     LLGIHKLKTKYRATNWVEAVKADVFLLSNYSKHPNFREVLPRVGNYNFLYEIFYQFRV
                     NICILDKDLTKNTMKLSVVFYKDNKKKLVVVGLKRDETGVFRPATLHESRNNPYKRIR
                     HTAIKSITWI"
     gene            complement(61945..62745)
                     /locus_tag="VOX58_000163"
     CDS             complement(61945..62745)
                     /locus_tag="VOX58_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HES5877442.1"
                     /translation="MSKANRRIWQGLAVILIAILTTFTTSTATAAKKIRNFPTTTEIL
                     SGTKATEAPGILPFTGSYKLVLGDLDNLRRPTFAHIQLKDQDEPKIQRKGLKFNPPGW
                     HNYKLTDANGKTTWLMDRGHLVGYQFSGLNDEPKNLVTMTKYLNTGFSDRNPLGMLYY
                     ENRLDSWLALHPNFWLDYKVTPIYHENELVPRQVILQYVGIDEDGNLLQIKLGGEKES
                     VDNYGVTSVTLDNVSPLAELDYQTGMMLDSTQNEEDSNVETEEFEEAA"
     gene            62985..63167
                     /locus_tag="VOX58_000164"
     CDS             62985..63167
                     /locus_tag="VOX58_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HES5877443.1"
                     /translation="MLTYDEFKQAIDDGYITGDKVTIVRKNGQIFDYVLPHEEIRDWE
                     VVTDEKVEEVVVELDK"
     gene            63358..64356
                     /gene="ruvB"
                     /locus_tag="VOX58_000165"
     CDS             63358..64356
                     /gene="ruvB"
                     /locus_tag="VOX58_000165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HES5877444.1"
                     /translation="MARILDNDVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            64494..64931
                     /locus_tag="VOX58_000166"
     CDS             64494..64931
                     /locus_tag="VOX58_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HES5877445.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMSKEY"
     gene            64954..65355
                     /locus_tag="VOX58_000167"
     CDS             64954..65355
                     /locus_tag="VOX58_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877446.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
BASE COUNT        21297 a        11385 c        14442 g        18280 t
ORIGIN      
        1 gaagttgctg gtgtagatcg cttattaacg gttgatttac atgctgctca aattcaaggg
       61 ttctttgata ttccagttga tcacttgatg ggtgcacctt taattgcaga ctattttgat
      121 cgtcatggtt tagtgggtga agatgttgtc gttgttagtc ctgaccatgg tggtgtgact
      181 cgtgcgcgta aattagcaca attcctccaa acaccaattg caattatcga taagcgtcgt
      241 agtgtggata agatgaacac cagtgaagtg atgaatatta tcggaaatgt ttcgggcaag
      301 aaatgtatct tgattgacga tatgattgat accgcaggga ctatttgcca tgcagcagat
      361 gcgcttgctg aagcgggggc aacagctgtg tatgcgtcat gtacgcatcc cgttctttct
      421 ggaccagcac ttgataatat tcaacgttcg gctatcgaaa aactaattgt tcttgatact
      481 atttatctgc caaaagaacg ccttattgat aaaattgaac aaatttcaat tgctgatttg
      541 gttgcagaag cgattattcg cattcatgaa aaacgtccgt tgtcaccttt atttgagatg
      601 ggcaattaaa cttaacgaag cctgagataa tagatctcag gctttttgac ttgaataaaa
      661 tgtaagggta atgtggtatt aattattgtg ggatgaacta tgcaattcta aaggattttg
      721 ggtataataa gaaagataac aacagtttat tgtaaactaa gataaaaggt aagagaaaaa
      781 tacgacgagg taatatgcaa ctaacagaat cactaggcat cgttcttttt aataggaatt
      841 atcgagaaga tgataaatta gtcaaaatat ttactgaagt agcaggtaag cagatgtttt
      901 tcgtgaaaca tattagtcgt tccaaaatgt cctcaatcat tcaaccacta acgattgctg
      961 attttatttt caagttaaat gatacaggct tgtcttatgt tgttgactat agtaacgtta
     1021 acacttatcg gtatattaat aatgatattt ttcgattagc ctatgctagt tatgtcttag
     1081 cattagctga tgctgcgatt gcagataatg aatcagattc gcatttgttt acgtttttga
     1141 aaaaaacact tgatttgatg gaagagggcc tagattatga aattttgaca aatatttttg
     1201 aaattcagat attagatcgt tttggtatta gtctaaactt tcatgagtgt gccatttgtc
     1261 atcgcactga tttaccgctt gatttttccc atcgtttttc agctgtactt tgttctgaac
     1321 attattacaa agacaaccga cgtaatcatt tagatccaaa tgttatctac ttgttgagtc
     1381 gatttcaaaa aatcacattt gatgatttga gaactatttc attgaataaa gacatcaaaa
     1441 agaagcttcg tcagttcatt gatgagttgt atcacgacta tgtaggaatc aaattaaaaa
     1501 gtaaaacatt cattgataat ttagttaagt ggggagatat tatgaaatga tagggcctct
     1561 ttcgtttatg ggaattggtt tcagaatatg ctataataaa aatactatta atagattgtg
     1621 taagaacaca agatataatt aaaggagtgg aaatgaagag aattgcaata gatgctatgg
     1681 gtggagataa cgctcctaaa gctattgttg agggtgttaa tcaagcaata gaagcatttt
     1741 cagatatcga aattcaactt tacggtgatc agacaaagat taactcctat cttattcaat
     1801 cagatcgtgt tgctattatt catacagatg aaaaaattat gtcagatgat gaacctgcaa
     1861 aagctgttcg ccgtaaaaag aaggcttcaa tggttttagc agctaaggca gtgaaagaag
     1921 gaaaagcaga tgccattatt tcagcaggta atacaggtgc tcttttagct gtaggtttgt
     1981 ttgttgtggg gcgtatcaag ggagttgatc gtccaggatt attatcaact attcccacag
     2041 taaccggatt aggatttgat atgttggatc ttggagctaa tgctgaaaat acggcaaaac
     2101 atttacatca atatgctatt ttaggctctt tctatgctaa gaatgtacgt gggattgcta
     2161 atccacgtgt tggtctatta aacaatggta cagaagaaac aaaaggagat ccccttcgta
     2221 aggccactta tgaactttta acagcagata atacaatatc ttttgttggt aatgtcgagg
     2281 cgcgagaact tatgtcaggt gttgcagatg ttattgtatc agatggtttc acgggaaacg
     2341 cagtgttgaa atcaatagaa ggaacagcta ttagcattat ggggcaactg aaacagataa
     2401 ttaatagcgg cggtattaag actaaaattg gagctagtct acttaaaagt agtctttacg
     2461 agatgaggaa gactcttgac tactctagtg ctggaggtgc agttttattt ggtttaaagg
     2521 cacctgttgt aaagtctcat ggttctagtg atgtaaaagc tattttttca acgataaaac
     2581 aagtacgtac gatgctagac actaacgtgg tcggacaatt agtagaagaa tttgctaagg
     2641 agactcaagt aaatgactag acaggagatt tttgaaagac tgattaactt aattcagaag
     2701 caaagaagct atttgagtgt agctattaca gaacaaacac atttaaaaaa tgatttagcg
     2761 gttgactcta ttgaactagt tgaatttatt attaatgttg aagatgaatt tcatcttgct
     2821 attcctgatg aagatgttga ggatatggta tttatgggag acgttttaga ttatttagtt
     2881 cagcgtttga cttagttaga ataataaaaa gagcgtaagc tctttttatt attctttaaa
     2941 aacgaacctt tttttaaaaa aacttataaa cgtaaataat atcttgaata tttttataga
     3001 gtatattact cttaaatgaa aacagaaaga aaggcgaata atcaagtgac taaccagctt
     3061 atttataaag gaaaagcgaa agatatttat agtacaaaag atgaaaatgt gattagaacg
     3121 gtctataaag accaagctac tatgttgaat ggagctcgta aggagactat tgatggcaag
     3181 ggagcactaa ataatcagat ttcatctctt atttttgaga aattaaataa ggctggtgtg
     3241 gtgacacatt atattgagca aatttcaaaa aacgaacaat tgaataaaaa agttgatatt
     3301 attccacttg aggtggtgtt acggaatgtg atagcaggct cattttcaaa acgttttgga
     3361 gtggaagaag gacgtgttct tgaaacgcca attgtggaat tttactacaa aaatgatgat
     3421 ctgaatgatc cttttatcaa tgatgagcat gtgaagtttt tgggtattgt taatgatgag
     3481 gagattgctt atctgaaagg agagactcgc cgtattaatg aactcttgaa aggttggttt
     3541 gctcagattg ggcttaactt gattgacttc aagttggagt tcggatttga tcaagaaggt
     3601 acaattatct tagcagatga attttcgcct gataactgtc gcctatggga taagaatggt
     3661 aaccatatgg acaaggatgt ttttagaaga gaccttggca acctgacgga tgtctatcaa
     3721 gttgtacttg agaagttgat agcattgtaa ctagaagagg aaggaattga atccgttcgg
     3781 gaaactccga aaaaaagatg gaacttactg aaatctcaat ttcagatgtc ctcctatttt
     3841 ccagtcgctc ccttgtcgga cttggtatct tatatgaata aacgtatttt tgttgagaaa
     3901 aaggctgact ttggtattaa atcggctagc cttgtgaaag agttgacgca taatctacaa
     3961 ctgacttctt tgaaggcttt gcgtattgtg caggtctatg atgtcttcaa tttggctgag
     4021 gatttgctgg cgcgtgctga gaagcatatt ttctctgagc aggtgacaga ctgtcttttg
     4081 acggaaactg aaatcactgc ggagcttgat aaggttgcct tctttgccat tgagtcgctt
     4141 cctggtcaat ttgaccaacg tgctgttagt tcgcaagaag ctttgctatt atttggaagt
     4201 gacagtcagg ttaaggtcaa tacagcccag ctttacttgg tcaataagga tattgcagaa
     4261 gcagagcttg aagccgttaa gaactatctt ttgaaccctg ttgattcgcg tttcaaggac
     4321 attactttgc cgcttgaaga gcaggctttc tctgtgtctg ataagacgat ccctaatctt
     4381 gatttcttcg agacttataa ggctgacgat tttacggctt ataaggtaga gcagggcttg
     4441 gctatggagg tcgatgatct tctcttcatc caagattatt tcaaatcaat cgggcgtgtg
     4501 ccaactgaga ctgagttgaa agttttggat acgtattggt cagaccactg tcgtcacaca
     4561 acctttgaaa ctgaattgaa gaacattgat ttttcagctt ctaaattcca aaaacaattg
     4621 cagacaactt atgacaaata tatcgccatg cgtgatgagc ttggtcgttc tgaaaagcca
     4681 caaacactta tggatatggc gactattttt ggtcgttatg agcgtgccaa cggtcgtctg
     4741 gacgatatgg aagtctcaga tgaaatcaat gcctgctcag ttgagattga agtagatgtt
     4801 gatggtgtga aagagccttg gctcctcatg tttaagaatg agacacacaa tcacccaaca
     4861 gaaattgagc cattcggtgg agcggcgact tgtatcggtg gtgctattcg tgacccattg
     4921 tcaggacgtt catacgttta tcaggctatg cgtatttcag gcgcaggcga tatcacgact
     4981 ccgattgcgg aaacacgtgc tggtaaattg ccacaacagg ttatttctaa gactgcggcg
     5041 cacggctatt cttcatatgg taaccaaatt ggacttgcga caacttatgt gcgtgagtac
     5101 ttccaccctg gcttcgtagc caaacgtatg gagcttggag ctgtggttgg tgctgcacct
     5161 aaggaaaatg tggttcgtga aaaaccagaa gcaggtgatg tggtaatctt gcttggtggt
     5221 aaaacagggc gtgacggtgt cggcggtgcg acaggttcat ctaaggttca aacggttgaa
     5281 tctgtggaaa cagcaggagc agaggtacaa aaagggaatg ccatcgaaga gcgtaagatt
     5341 caacgtcttt tccgtgctgg caatgtcact cgtctgatca agaaatcaaa tgacttcggt
     5401 gcaggtggtg tctgtgttgc catcggtgaa ttggctgacg gtcttgaaat cgatttggac
     5461 aaggtgcctc ttaaatacca aggtcttaat ggtactgaaa ttgcaatctc agaatctcaa
     5521 gagcgtatgt cagtcgttgt tcgtccaagt gatgtggatg ccttcatcgc agcctgcaac
     5581 aaggaaaata tcgatgcagt cgtagttgcg actgtgactg aaaaaccaaa tcttgtcatg
     5641 acttggaatg atgaaacaat tgttgatttg gaacgccgtt tccttgatac caatggtgtc
     5701 cgtgtcgttg ttgatgctaa agtcgttgac aaggacttga cagttccaga agcacgcata
     5761 acatcagcag agacacttga agcagatacg cttaaggtct tgtctgacct caaccatgct
     5821 agtcaaaaag gtctccaaac catctttgac tcatctgttg gtcgttcaac cgttaaccac
     5881 ccaatcggtg gtcgttacca aatcacaccg acagaaagtt ctgttcaaaa attgccagtt
     5941 caacatggtg tgacaacaac tgcatctgtt atggctcaag gttacaatgc ttatattgca
     6001 gagtggtcac cttatcacgg tgctgcctat gctgttattg aagcgacagc tcgcttggta
     6061 gcaacaggtg ctgactggtc tcgtgcacgt ttctcttacc aagagtattt cgagcgtatg
     6121 gataaacagg cagagcgttt tggtcagcca gtatcagctc ttcttggttc tattgaggct
     6181 cagattcaac ttggcttgcc atcaatcggt ggtaaggact ctatgtctgg tactttcgaa
     6241 gacttgacag taccaccaac cttggtagct ttcggtgtga caacagcgga cagccgcaag
     6301 attctctctc ctgagtttaa agcggctggt gagaatatct actacattcc aggtcaagct
     6361 atttcagaag atattgattt tgaccttatc aaggctaact tcagtcagtt tgaagctatt
     6421 caaactcaac ataagattac agctgcctca gccgttaaat acggtggtgt cctagaaagt
     6481 tttgctctca tgacttttgg taaccgtatc ggtgcttctg ttgaaattgc agagcttgac
     6541 agcagcttga cagctcaact cggaggtttt gtctttacat cagctgagga aattgctgac
     6601 gcggtgaaaa ttggtcaaac tcaggcagac tttacagtca ctgtcaatgg aaatgacctt
     6661 gctggcgcta gccttctagc agccttcgaa ggcaaattgg aagaggttta cccaacagaa
     6721 tttgaacaga cagatgttct tgaagaagtt cctgctgtgg tatcagatac tgttatcaag
     6781 gctaaggaaa caattgaaaa accagtggtt tacattccag tcttccctgg taccaactca
     6841 gagtacgatt cagctaaggc ctttgaacag gttggagcta gtgtcaactt ggtaccattt
     6901 gtaaccttga atgaggttgc tattgctgag tcagttgaca ctatggttgc taatattgct
     6961 aaggcaaata tcatcttctt tgctggaggt ttctcagcag cggatgaacc agatgggtct
     7021 gctaagttta tcgtcaatat cttgcttaac gagaaggtcc gcgcagctat tgacagcttc
     7081 atcgaaaaag gtggccttat catcggtatc tgtaatggtt tccaagccct tgttaaatca
     7141 ggtcttcttc catacggaaa cttcgaggaa gctggtgaga caagtccaac tctcttctat
     7201 aacgatgcta atcagcatgt tgccaagatg gttgagactc gtatcgcaaa taccaactca
     7261 ccttggttgg caggagttga ggtcggcgat attcatgcca ttccagtttc acatggtgaa
     7321 ggtaaatttg ttgtcagcgc ttctgaattt gcagagctaa gagacaatgg tcaaatctgg
     7381 agccaatatg tggactttga cggacaacca tctatggatt ctaaatacaa tccaaacggc
     7441 tctgtcaatg ccatcgaagg gattaccagc aagaatggtc aaatcatcgg taagatggga
     7501 cactcagaac gctgggaaga cggactcttc caaaatattc ctggtaacaa agaccaaacc
     7561 ctctttgcaa gtgctgtaaa atactttaca gggaagtaaa aaggttcagt gaaccttttt
     7621 agccctcgcc ttgaattagt gaagggcaag gttagaaaga atctcgctta atttttgaga
     7681 aatagtaaac acgggcgata agttgtgcga aaaactttct attttcgctt tacttgacag
     7741 cctttgtatc gaggtaaaaa tgacatacga agtaaaatct ctaaatgaag aatgtggagt
     7801 ctttggtatc tggggacatc ctcaggcagc tcaagtcact tactttgggc ttcatagcct
     7861 tcaacatcgc ggtcaagaag gagctggtat tgtttcaaat gacaatggga aactctatgg
     7921 ttatcgaaat gttgggctcc tttctgaagt ttttaagaat caatctgaat tagataattt
     7981 aactgggaat gcggctattg gacatgttcg gtatgctact gcaggttctg cagatattcg
     8041 caatattcag ccttttcttt ataaatttca tgacgggcaa tttgctttat gccataatgg
     8101 taatttgaca aatgctattt ccttaaggaa agaattagaa aagcaaggtg caattttcaa
     8161 tgcctcctca gatactgaaa ttttgatgca cttgattcgt cgaagccata actcaagttt
     8221 catgggaaag gtaaaagaag ctctaaatac agtaaagggt ggctttgcct atctactgat
     8281 gacagaaaat aaactcattg ctgctcttga ccctaatgcc tttcgtcctt tgtcaattgg
     8341 acaaatgcaa aatggtgcct gggttatttc cagtgagacc tgtgcttttg aggtggtagg
     8401 cgcaaaatgg gttagagatg ttgaacctgg cgaagttatt cttatagacg atcgtggtat
     8461 tcaatgtgat cgttatactg atgaaacgca actggcaatt tgttcgatgg aatatgtcta
     8521 ttttgcaagg ccggattcaa ctatacatgg tgttaatgtt catacggctc gaaaaaatat
     8581 gggaaagcgt cttgcacaag aatttaaaca ggatgctgat attgtaattg gtgtcccaaa
     8641 ttcgtcctta tcggctgcta tgggctttgc tgaagaatcc ggattaccaa atgagatggg
     8701 tcttgtaaaa aatcagtata cgcagcgaac ctttattcaa ccgacacaag aattaaggga
     8761 acaaggtgtt cgaatgaaac tatcagcggt atctggtgtt gtcaaaggaa agcgcgttgt
     8821 tatgattgat gactcaattg taagaggaac gacttctaga aggattgtcg gattattaag
     8881 agaagcagga gctagtgaag tacatgttgc tatagctagt ccagaattaa agtatccttg
     8941 tttttatggt attgatattc aaacaagacg agaattaatt tcagcaaatc acgctgctga
     9001 cgaagtgtgt gatattattg gcgcagatag tctgacctat ctctcactag atgggttgat
     9061 tgaatcaatt ggactcgaaa caaaagcacc aaatggtggt ttatgcgtag cttactttga
     9121 tggacattat ccaacaccgc tttatgatta tgaagaagaa tacctcagaa gtttagaaga
     9181 aaaaacaagt ttttatattc aaaaagtgaa ataagctctg ctaattatat aaggagaaaa
     9241 catgtctgaa aaaaatgctt atgcaaaatc tggtgttgat gttgaagctg gttatgaagt
     9301 ggttgaaaga ataaaaaaac acgttgctcg tacagaacgt gcgggagtca tgggagctct
     9361 aggtggcttt ggtgggatgt ttgacttgag taaaacagga gttagagagc ctgttttggt
     9421 ctcagggaca gacggtgttg gcacaaaact catgcttgct atcaagtacg acaagcacga
     9481 cacaatcggt caagactgtg ttgccatgtg tgtcaatgat attattgcag caggtgctga
     9541 gcccctttac ttccttgact acgtagcgac aggaaaaaat aatccagtca aatttgaaga
     9601 agttgtttct ggcgttgcag aaggttgtgt tcaagcagga gcagcactca ttggaggtga
     9661 gaccgctgaa atgcctggga tgtatggcga agatgattat gaccttgctg gctttgctgt
     9721 tggtgtggct gaaaaatctc agctcatcga cggttcaaaa gtaaaggaag gcgatattct
     9781 tttgggactt gcttcaagtg gtatccattc aaacggttat tctttggtac gtcgtgtctt
     9841 tgctgactat actggtaaag agctgcttcc agaacttgaa ggaaaacaac ttaaggatgt
     9901 ccttcttgag ccaactcgta tttatgttag agcagctctg ccattaatca aggaagaact
     9961 aatcaacggt atcggccaca tcacaggtgg tggctttatc gagaatgttc ctcgtatgtt
    10021 tgcagatgat ttggctgcgg aaatcgatga ggacaaggtg ccagtgcttc cgattttcaa
    10081 ggcgcttgaa aaatatggtg acatcaagca cgaagaaatg tttgaaatct tcaatatggg
    10141 tgtcggtctt atgctagctg ttagtcctga aaatgttaac cgcgtcaaag agctcttgga
    10201 tgaaccagtt tatgaaatcg gtcgtatcat caagaaagca gacgatagtg tggtgattaa
    10261 ataagtcact aaattttata agaaaggagt gtttagttag tttgcctaac tgaacacggg
    10321 attaaatttc taggaaaaaa gataaaatga cttagacgca agcgtcgttg ttcgttttcc
    10381 tatttttcta cgaaattttt cggcaagccg aatcatccct ttgtatcgaa tatgaaaatc
    10441 gctgtttttg cttctggtaa tggttccaac tttcaggtca tagcagagca gtttccagtt
    10501 agttttgtct tttcagatca tcgtgatgcc tatgttttag agcgtgctca gaatttagcc
    10561 attccaagct tcgcttttga actcaaggag tttgagaata aggcggctta cgagcaagcc
    10621 atcgttgatt tgttggacaa acacgagatt gatttggtct gtctaacggg ctatatgaag
    10681 attgttggag aaaccttact cttggcctat gaggggcgta ttatcaatat tcacccagcc
    10741 tacctgcctg aatttccagg tgcccacggt atcgaggatg cttgggaagc tggtgttgac
    10801 cagtctggcg tgaccatcca ctgggttgac tctggtgtgg ataccggtca ggtcatccaa
    10861 caagtgcgcg tgccacgcct agcagacgat agcctagaaa gctttgaaac ccgtatccat
    10921 gaaaccgaat atcaactcta cccagctgtc ttagatagct tgggagtgga gaggaaagta
    10981 atctagcctt tatcttaaaa ttttaggtaa agttaggaaa gatagtaaag aagaattaac
    11041 gttccaaaaa gaaaggaatt gaacccgtct ggaaggcaca gaaaaaagat aaacatctcc
    11101 taggcgcaag cgtcttcgtc gagtttccta tttttccttg gccttctagt cggacttggt
    11161 atcttaaata tgactaaacg tgctttaatc tcagtgtctg acaagtcagg aattgttgac
    11221 tttgcaaaag aattgaaaaa cttgggttgg gatattatct caactggtgg gactaaggtt
    11281 gcccttgata atgctggcgt tgagaccatt gccatcgacg atgtgactgg attcccagag
    11341 atgatggacg gtcgtgttaa gaccctccac ccaaacattc acggtgggct tttggctcgt
    11401 cgcgacgctg acagccacct tcaggctgct aaggacaaca atatcgagct gattgacctc
    11461 gtggttgtca acctctatcc cttcaaggag actatccttc gtccagatat aacctatgat
    11521 ttggcggtgg aaaacatcga catcggcggt ccatcaatgc ttcgctcagc cgctaaaaat
    11581 cacgctagcg tgaccgttgt ggttgaccca gcggattatg cgactgtttt gggagaattg
    11641 gctgacgctg gtcagacgac atttgaaacg cgtcaacgct tggcagctaa ggtctttcgt
    11701 cacacagcag cctacgacgc tttgattgct gagtacttca caactcaagt gggagaggct
    11761 aagcctgaaa aattaaccat cacttacgac cttaaacagg ctatgcgtta cggagaaaat
    11821 ccacaacaag acgctgattt ctatcaaaaa gccttgccaa cagactactc aattgcttct
    11881 gctaaacagc tcaacggaaa agaattgtcc ttcaataata tccgtgatgc cgatgcagca
    11941 atccgtatta tccgtgattt caaagatcgt ccaactgttg ttgtcctcaa acacatgaac
    12001 ccatgtggta tcggacaggc tgatgatatt gagacagctt gggattacgc ttatgaagct
    12061 gacccagttt caatctttgg tggaattgtc gtccttaacc gtgaagttga cgcagcgaca
    12121 gctaagaaga tgcaccctat cttcttggaa atcatcatcg caccatcata ctcagaagaa
    12181 gcgctagcta ttctcacaaa taaaaagaaa aacttgcgaa tccttgagtt gccgtttgat
    12241 gcccaagctg ccagcgaagt ggaagctgag tacactggcg tagttggtgg acttttggtg
    12301 caaaaccaag acgttgtggc tgaaaatcca tctgactggc aagtggtgac agaccgccaa
    12361 ccaacagaac aagaggcgac tgcccttgag tttgcctgga aggctatcaa gtatgttaag
    12421 tctaacggga ttattattac taacgatcac atgacgcttg gactcggtgc aggtcaaacc
    12481 aaccgtgtcg gctcagtcaa gattgctatc gagcaggcta aagaccacct tgacggtgcc
    12541 gttctagcat cagatgcctt cttcccattt gcggacaaca ttgaagaaat cgctgccgca
    12601 gggatcaaag caatcatcca accaggtggt tcagttcgtg accaagactc tattgacgcc
    12661 gcaaacaaac atggcttgac catgatcttc acaggtgtga gacattttag gcattaataa
    12721 aaaaatgtgg tctaattaaa accacatttt ttattaaaag aaaatataag ttaactataa
    12781 tttgttagct ttatcagcat ttacccatgt tccacgagat ccgatttgaa cataaatacc
    12841 attacttggt tgatcaattt ttaatacttt ataactacca gtgacggtaa aatattcacc
    12901 cacacggaga atttgatctc ctaaaacttt tccttggtta tctgtttcat ctaatggtgt
    12961 gggatcaatc cagtttagtg gagttgggtc tcctccggct aattctttat taacgatcaa
    13021 attattaaca agctgatcta cacgaaaaac gccagggaag gtaacagtat caccgacctt
    13081 aatggaaggt tttgtattgt cattttgaac cacagactgt gcgggctctt taatgggaat
    13141 atagcgtcgg tttccagaaa aactgaggta gctaagccat gtataacctc cagcagtcac
    13201 gactttatcg taataaacag attgacctgc ttcatagtaa gcaagatcag ggctatcgat
    13261 actggtttgt cctttgactg gtaatctggt tgtaaagtga taagttccag aggggtcaaa
    13321 tgaagcttga gaaatgtgtt ttagttgtct tgggtaggaa tgacttgtct gagatgataa
    13381 gtctttaaaa tggatataac cacttacctg agattttgga atttgacgct tatggtatct
    13441 ttcagggcct tgtccagcgt tgtaattata ctcttcgata gtgacagtgt ctccacggat
    13501 atcagctacc catgctacat gaccgtaagc agcatttgac tgataagcgt ttttatcaaa
    13561 ccaagcgata gcccctatgc ttggtgtctt attcacagga taaccctgat ttttcgcgat
    13621 atgtccccac gtgcaggcat taccgtagcc tttaggtaac tgaaaaccat tagcagagct
    13681 taaacgaaaa gctgcaaaag aagtgcattg gcggatatac atgttccacg aatcgattcc
    13741 attgcctttt ttccacttac tcggatagtt atcgcctaaa acagctgcat gaacaagatt
    13801 ttcctgttgc gaaataagtg tagatggcat agtaggtact agaccattaa aacctaaaag
    13861 gattactcct gagaccaaaa aacgatgaaa ttttttcatt aattacctcc tgtaacttat
    13921 tattcgaaag caagaaacaa aaaatgttat ttttagatga ttattttgaa aattggcttt
    13981 acttgatatt aaataactgt ttgattggct aatagagtgt tccttatttc aagaattgtg
    14041 gtctactatc acatttttac aatgtttcaa ttattgataa ctaatacatt atgtaaaaaa
    14101 gcgaacgttt atgatataat atctaattat aatacgaaag aggtggatgt tttgaaatta
    14161 cttgttgttg gttcaggtgg tcgtgaacat gcgattgcta agaagttgtt agcgtctaag
    14221 ggtgtggatc aggtttttgt ggcacctggt aatgatggta tgaccttgga tggtctggac
    14281 ttggtgaata tagtagtttc cgaacattcc aggttgattg cctttgctaa ggagaatgag
    14341 atttcttggg cctttattgg tcctgatgat gcgctagcag ctggtatcgt tgatgatttc
    14401 aacagtgctg gactcagagc tttcggtcca acaaaggcag cagcggagct agagtggtca
    14461 aaagactttg ccaaggaaat catggtcaaa tacaatgtcc caacagcagc ctatggcaca
    14521 ttttcagact ttgaaaaagc caaagcctac atcgaagagc agggcgtacc tatcgtggtc
    14581 aaggctgatg gcttggcgct aggtaagggt gtggtcgtgg ctgaaaccgt tgagcaggcg
    14641 gtagaggcgg cgcaagagat gcttctggac aataagtttg gcgattcagg agcgcgtgtg
    14701 gttatcgagg aattcttgga tggcgaggag ttttccctct ttgcctttgt taatggtgat
    14761 aagttctaca tcatgccgac agctcaggat cacaagcgtg cctttgacgg agacaagggg
    14821 ccaaacactg gtggtatggg tgcctatgcg ccagttcctc atctacctca gagcgtggta
    14881 gacacagcgg ttgagacgat cgttaggcct gtccttgagg gcatggttgc tgaagggcgt
    14941 ccttatctag gtgtcctcta tgtggggctt atcctgacag ctgatggacc taaggttatc
    15001 gagttcaact cacgtttcgg tgaccctgaa actcagatta tcctccctcg cctgacttcc
    15061 gatttcgctc agaatattga cgatatcatg atgggcattg agccttacat cacttggcag
    15121 aaggacggtg tgactttggg tgttgtcgtt gcctcagaag gctatccact cgattatgag
    15181 aaaggtgtgc cactgcctga aaagaccgac ggcgatatca tcacctacta tgccggagct
    15241 aagtttgtgg aaaatagcga gctacttctc tcaaacggag gacgtgtcta tatgcttgtc
    15301 accacagaag acagcgtcaa agcagggcag gacaaaatct atacccagct agcccaacaa
    15361 gacacaacag gtctcttcta ccgaaatgac atcggaagca aagctataag agaataagac
    15421 taaccattca aatattagcg tcagcaatca acgaccgaag gtcgctccaa aaagaaagaa
    15481 gaaaggaact gaacacggga ctaatttcct aggaaaaaaa taaaactacc tggacacaag
    15541 cgtcatcgtt agttttctgc gtcgtaggcg tctgatgcta aagcacctag tcgccctgac
    15601 gtgggttcgt cttcgaaatc aaagatttcg gacttaccgc atatttttct acggaaattt
    15661 tcgacaagtc gaaacgcccc tttgtatctt aattatgaaa actccaatta tttctattat
    15721 catgggctcc aaatctgact gggcaaccat gcaaaaaacg gctgaagttc tagacaactt
    15781 tggcattgct tacgagaaga aagtcgtctc tgcccaccgt acgccagacc tcatgttcaa
    15841 gcatgctgag gaagcacgtg gtcgtggcat caaaatcatc atcgcaggag caggtggggc
    15901 agctcacttg cctggtatgg tggcagctaa aacaaccctg cctgttatcg gtgtgcctgt
    15961 caaatcacgt gccctttcag gtttggattc actctattcg attgtgcaga tgcctggcgg
    16021 tgtgcctgtg gcaaccatgg ctatcggaga agcgggagcg actaacgctg ccctgacagc
    16081 cctccgtatt ctctccatcg aggatcaaaa tctggctgat gcgctagctc atttccatga
    16141 ggaacaagga aaaatcgcag aggagtcaag tggtgaactc atttaagacc attgggatta
    16201 tcggtggtgg tcagctgggg cagatgatgg ccattgcggc tatctatatg ggctacaagg
    16261 tcatcacgct ggatcctgct agcgactgcc ctgcctcccg cgttagcgag gtaattgtgg
    16321 cgccttacga tgatgttgag gctttgggac aattggctgc gcgttgcgat gtattgactt
    16381 atgagtttga gaatattgat gcggacggtt tggatgccgt tgtgtcagct tgtcagctac
    16441 cgcaggggac cgatctgctc cgcatttccc aaaaccgtat ctttgaaaaa gactttctgg
    16501 caaataaggc tggtgtgact gttgctccct ataaggtggt gacgtctagc cttgacctag
    16561 ggggacttga ttttaccaag acctatgtcc tcaagacagc gacaggtggt tatgacggtc
    16621 atgggcaaaa ggttatccgc tcagcagaag atttgccaga ggcgcagcaa ttagctaact
    16681 cagctcagtg tgtcttggaa gagtttgtca actttgacct tgaaatctca gtcatcgtgt
    16741 ctggaaatgg agaggatgtg acggtctttc ccgttcagga aaatatccac tgcaacaata
    16801 tcctgtcaaa aactatcgtg ccagctcgta tctcagatca actggctgat aaggctaaga
    16861 aaatggcggt gcagattgcc aagaaactcc agctgtcagg aactctctgt gtggaaatgt
    16921 ttgcgaccgc agatgatatc attgtcaatg aaattgcccc acgtccccac aactcagggc
    16981 actactcaat cgaggcttgc gacttttcac agtttgacac ccacattttg ggcgtgctag
    17041 gagcgccgct cccaaccatc cacctccatg ctccagccgt catgctcaat gtcctcggac
    17101 aacacgtcca gcaggcaatt gactatgttg cccaaaaccc tagcgcccac ctccacatgt
    17161 atggtaaact agaagcgaaa cataaccgca aaatgggaca tgtgacggtg tttagtgatt
    17221 tgcctgatga ggtggaggag ttttaggatg tatctaaagt atatatttaa aataaaggat
    17281 aggaatttag tatcggatta tgaatcttta gttcgaaatt tgacaatcaa tcttcaaaaa
    17341 ctagttatta aagatagaac tgcattaata ggggataaac aacttccaat tacgatatat
    17401 tatcgtgggg agaatgaaaa gtttcctacg ggaatagtaa aagttgaatc agactcagtt
    17461 gaaggattag agttatttga taaaacgata aaggaaatag ttgggaatga aagtagattt
    17521 ggaaagttca catgtatata cgatggaata agtacatatt attcaacaga aatttattct
    17581 gaacttcatg aaattgagcg tcgaacacaa gagtttttaa gggaagcatt ttttctgagc
    17641 gataaaagtg gggaattgaa agaatatcag gcaactatca aaaaaatgga tagtagtgat
    17701 ttctcattca gcaaattaat taatgaaatt tttaaagcaa gcgatataac tgatacattt
    17761 attgaccatg ttagagcgtg tgttgaattt ggaacagttc caaatggaga tttattacca
    17821 aaaagttttt gggatagttt agatgactct ttactagatg ataaagagca aggtttagag
    17881 attaataatt tattaaatga gattcgttca acaagaaata caatagctca ctgtaaagag
    17941 tttaaaaaga aggattatat taattgtata gaacaagtta aaaaattaaa gcaagcattg
    18001 gataaaataa tagcctctat agagtctaca gatatttcta aaatgtcacg agaagtgtca
    18061 cgcataaata actccttaca agccgataat gactacatca aacaaaaata ttttatagaa
    18121 tatgatattt ttgaaaagga tatattagct aggacagggt tattagaaat tgattgggaa
    18181 aacgtttctg atgacataga gcccttaaag atacatcgta acttttatga atcaattagt
    18241 gaaaaaacag attctgaagt tatagaagaa atcaaaaatt tagaagatat aaatttcact
    18301 gatgaaaagg gtaatgattt ggatgactta accattattg ttcctgcgca agacgaagga
    18361 tttaaaagag tctttctagg tcaacgagaa tggtatgata tacggatagg aaaatcgaaa
    18421 aggaatagga ttaaatatat cgcaggatat gaagttgcac cagagagtgg tgtaaagtac
    18481 tgggctgaag tggataaaat agttccttca gataactatg ttggttactg gaaagtaatt
    18541 tttaaaggag aggtgaagga atattcagaa tataaaagat taggaaatac ttggccccct
    18601 caaaacagta gatacgtatc taaaaaggct ttagatgaag ctaaatattt atcggatgtt
    18661 ttttaattaa aggagaaaaa acaaaatgat cgaacgttat tcacgccctg agatggcggc
    18721 aatttggaca gaggaaaata aataccgtgc ttggttggag gttgagattc tggctgacga
    18781 ggcatgggca gagttgggtg agattcctaa ggaggatgtg gctaagattc gtgagaaggc
    18841 ggattttgac attgaccgca ttcttgagat tgagcaggag acgcgtcacg atgtggtggc
    18901 tttcacgcgt gcggtttctg aaacgcttgg tgaggagcgc aagtgggtgc actacggttt
    18961 gacctcgact gacgtggtgg acactgccta cggttacctc tacaagcaag ctaacgacat
    19021 tatccgtcgc gatcttgaga atttcaccaa tatcgtggca gacaaggcgc gtgagcacaa
    19081 aatgaccatc atgatgggtc gtacccacgg tgttcacgcc gagccaacga ctttcggtct
    19141 taagttggcg acttggtaca gcgagatgaa acgtaatatt gagcgttttg aacatgctgc
    19201 tgcaggtgtg gaagctggta agatttcagg tgccgttggt aactttgcca acatcccacc
    19261 ttttgtggaa gaatatgtct gtgacaaatt aggcattcgt ccgcaagaaa tttcaacaca
    19321 agttcttcca cgtgaccttc acgcagaata ctttgcagtg cttgcaagta ttgcaacttc
    19381 tatcgaacgt atggcgacag agattcgtgg tctgcaaaaa tcagaacaac gtgaagttga
    19441 agagttcttt gccaaaggtc agaaaggtag ctctgctatg cctcacaaac gcaacccaat
    19501 cggttcagag aacatgacag ggctagcgcg cgtgattcgt ggtcacatgg tgacagctta
    19561 tgagaacgtg tcactttggc atgagcgtga tatttcacac tcatcagctg agcgtatcat
    19621 cacacctgac acaactatct tgattgacta catgctcaac cgctttggca atatcgttaa
    19681 gaacttgact gtcttcccgg aaaatatgat gcgcaatatg gaatcaactt ttggtttaat
    19741 ttatagtcaa cgtgttatgc tcaaattgat tgaaaaagga atgacacgag aagaagctta
    19801 tgacttagtt caacctaaga cagcttattc ctgggacaat caagtggatt tcaaaccact
    19861 tttagaagaa gacaccaaag ttacctcttg tcttacacaa gaagaacttg atgaactatt
    19921 taatccgatt tattacacaa aacgtgttga tgatattttt aagcgtttag ggatttaata
    19981 taaaaaaaag aaggtgattc ccttcttttt tatttaaaaa acgaatattt tctttaaaaa
    20041 tattaaaaat gttagttttt aattagacgc tgtgatataa taaagaaaac agttgttgga
    20101 gagaaaccat gttagaacat tttggtggaa aagtaaaagt gttaagactt gaaaagagga
    20161 ttagtcgcga ggacttgtgt ggggatgagt ctgaactttc tgttcgtcaa ttagcacgga
    20221 tagaactagg tcaatccata ccaagtttaa gtaaggttat ttttattgca aaagccttaa
    20281 acgttagtgt cggttactta actgatggtg ctgatttaga actacctaag cgttacaaag
    20341 aattaaaata ccttatctta aggacaccaa cttacatgga tgatggaaaa ttacaagtac
    20401 gagaagagca gtttgatgaa atttttgagg attattatga taaattacca gaggaagaga
    20461 aaataatcat tgattgttta caggcaactt tagatacttt attgagtgag aatactaact
    20521 ttggcattga cttacttcaa gaatatttta atcaaataaa gactaaggta cgctttaggc
    20581 aaaatgattt aatacttcta gaattatatt tagcttatct tgatattgag ggaatggatg
    20641 gacaatattc agataagatt ttttatgatt ctttattaga taatctatca gaacaatttg
    20701 aacaatttga attagatgaa ttatttatag ttaataagat tatcattgat atttcatcac
    20761 taagcttaaa aaataaccga cttgataatt tagaaaaagc gattgaaatg agtcaaaaaa
    20821 ttatggcgaa aattcaagat tggaatagaa tgcctatttt aaaactaata gaatggaaat
    20881 attttttaat caaacaaaaa gatataataa aagcagaaca atcatttatg aaggcatgtt
    20941 tgttcgccca aatgacagct gatcagtatt tggaaaacaa gttaattcaa gagtgggaaa
    21001 aagatgttaa aagttattaa caggacaaaa atgtcatttt taaatattta atagtaagat
    21061 ataattccta atataaaaaa taattattaa ggagttttta tccatgttaa aaaagtataa
    21121 gtactatttt atattcgcag ctctactatc ttttaaagta gttcaagagc ttagtgctgt
    21181 tgactggtgg cgattataat aaaagtaggc cactgctctc ttttacttta tgtcataata
    21241 tcatcacacc ttataaatcc cttgaaatca atgggtttct aaaaccaaaa atacgactta
    21301 ctacgttttt aactacgttc gtgttaaatt gcgataaaat cagctagctt ctcagctaca
    21361 ttatcggttt gatttttaga taggtgggtg tataaatcca ttgtaattga tattttggca
    21421 tgacctagac gttcttgtgc tagctttgca ggaactcccg cctcaaatag caatgatgcg
    21481 tgagtatgtc taaatccatg agggtttatt tttttaagat gatgccgttc aacggttctt
    21541 ttcagcctat gtctaaccgt gcttgtaagc agattaaata ctctaaaaga cccatgtagc
    21601 ggtgtgacac tctgcctcat atactgttta gccagtcgca ttgtttcctg atccatggaa
    21661 atagtgcggc tgcttttttg agttttagga ttttgaatga ccttacctac cttggtttgt
    21721 gctaaagttt ttgttacact gatagtgtta ttaataaaat caaaatcagt atcatataag
    21781 gcaacagcct cgccaattct taatccacca taggctaata accggtagat taacaacatt
    21841 tctagactct cttctttttt aacaatatcc aaaaagatta gtaattcctc cttagagtag
    21901 aacatttttt ctttatgggc ataggtacat ctagctctta ttgtcctatc cattgggtta
    21961 ttatcaataa ttcctaaatg tactgcaaac ttaaaaatcc tgttgataac actgatgtag
    22021 ctagcgcatg cgctatattg tgctaagtgg ttgattaagt tttgacaggc cataactgtt
    22081 attttattga tttgtgattt accaatatgc ggttttattg ttgacttata gtaattttgg
    22141 ttaaccagaa atgttgacgg tttgactgta tttcggtact gctcaaacca cagtttagct
    22201 acgtcatcaa atgtagtctt atcattgctt ttccaagcgc cttgacgctg aaaatcatcg
    22261 ataagtttta cctcggctct tttagcttct ctttctgttt taaacccttg tctagttgtc
    22321 ctgacttgtt taccagttat gggatcaaca ccaaggtatg ctcttaagcg ataggcagtt
    22381 gtgccatctt ttttagtgta tttttcaatc attgtttttt cctctctctt cgcgctgggg
    22441 agtgctgatt ttgagatagg atattggcat cacctcctaa aaatggtata attaggtaca
    22501 agaaaacgac ctttttaatg gttgtttctt atacggtttt gccccacgct ctcctcgcca
    22561 aatttgagcg tggggctttt tttatttgtc taaaagtaag tagccgtcta tataaattag
    22621 agggacacta ttctcagaac cacttgttgt cgtatattca taagataata aatatcttcc
    22681 attgatagtg atattatcag tttctaaaag cttagtttct agtcgcttgg tttcgataaa
    22741 caacatatac aagtccccat taggggtgtt tgctagaact ttagtatatt tatctttatc
    22801 agagatttgt attattttta aattattgat tctgacctta agggaaaagt actttttagg
    22861 gtctcttaaa attgttttgt agtcataaag tagatactcc tctgatttgt agttatctgt
    22921 tgttactttg tcaatcttag atattagagt atcaaagtat tctttttcgc tcatatctcc
    22981 agatacttta ctagtatccg atgatggagt ttttgatgtt gtatcttttt ttgagccacc
    23041 agagcaagct ccgagacata ctattaagat tgataaggca aacaaatata ctactttttt
    23101 cacaataaat cccctaaatt aaatttttaa attcttcttt gatcatcact tcgtcagcga
    23161 cattcctact caaactctat ttcagccatg ctatctgcga atgattccaa aatttcatgt
    23221 tcgttcatga aggaaaccca ctcatcaatt tcttcttggt gcatgaatcg tttcctgaac
    23281 ttcccgttta acttgatgtt gtcataaagt tgcttgacaa cattggtcca catagtctca
    23341 acttctttga gtaccttttg ctctttccat cgcttatgag cgggtgttcg cctagcattt
    23401 ttagcgtttt gataatcaag tgccctttca aagaaataat catgagcacg gctattgttt
    23461 tctacatact ctttgaattc gtcaaaatac attactttaa acctccgaga tattatacaa
    23521 aatgacgttt aaataattta tggctttcaa tgttctgatc gacatctttc tcatcccaaa
    23581 tttgtaactc ccaaggatag tagtggttgc tcttattctt gaagtaaacg tgtattcctg
    23641 tataactatc tttgtctcgt aaataccagt tttttaaatc aaacttatct ttccagtcat
    23701 cgagttgctc catcacttgt gaaatatctt cagaagatag aatgatacga gcgccaaaaa
    23761 tgtcattaag aatagaattc acaggataac cgtcttgtct ttttgaaaaa cgctcaattt
    23821 tgtctaagat gctttctgac gttttgacac gataaacgta aggtatgtct ttgacatcgg
    23881 ctttcatcaa ataatcattg atggattcgt gcaaattcaa gcggtaatct aaaatcgctt
    23941 gaacaggtac ttttgaaaag gtatgtttta gattaatctt ttcaactttc ccagtttcaa
    24001 aataatcttg cgaataaaca aggtgtattc tgttaatttc cgagattaag cgttcaactt
    24061 tttccagcat attaatcctc tctataaata tccacaactt ctccgatagt tctgatatta
    24121 tcatcttcag acaagtaaat ttcttcgtag ttgttgttca agctttgaag ataccaagcg
    24181 ccatcataat cacgcttgag tttttttaca aagtttttac cattgacttg gaatatccct
    24241 atatcattca tatcgacttg gctagatacc ttgatgaata gcaaatcgtt gtcttgaata
    24301 agtggttcca ttgaatcgcc agctactttt gcaatagtat catattcttc aggaacatca
    24361 ctagcacgca atctaacttc catatgtagg ttgtcttctt gaaaagtacc gttacctgcc
    24421 gcaacaagac cctcgacata atcattgata taatcctctt cttcgagctt tttatcgaac
    24481 atagaaacaa ctttattttg ttcttctaat tgttcgttag cgaagttgag gactttttct
    24541 tgtctaggtt gttctagttg gttgtagata gaaacgattt caggttgatt agatatttct
    24601 gtcgtagtga ttttatcttc gctattaata ggttgtaccc ctagtaaaaa ttcaggagtt
    24661 actcctagaa ctttagcgaa atcgttcgct ctatcaaggg ggaatcccct tgtcctgttg
    24721 aaatatcttg atagtccaga cttagccatc cctacctgtc ttgctaactc gctgatagat
    24781 aacctctttt cttttcttag ttcatcaata attgtgataa tttcttcatt agttcgcata
    24841 tttcactcct taaaggaatt ataccatcgt tccctaaaaa gtacaacaaa aacccctgaa
    24901 aaaacttttt aaactttttt cgatttattt gttgacaata aagaacaaaa gttgtaaact
    24961 aaaattgttc acttgagaga acaaaataaa ctacattgag aggaggattt attatgggag
    25021 ttgactattt acgagttaaa gctgaaagaa tagcaaaagg ttatactcaa gactatatgg
    25081 ctgagcggat gggttggtct gatagggcac gttatgcgaa acgtgaaaat ggaattgttt
    25141 catttgatgc tgacgaattg gcgaaagtag ttcacatctt aggattttca aaagatcaaa
    25201 taggaatttt ttttacagat agtgttcact aaagagaaca aaactaacga agggagaaaa
    25261 atatgccaga ggatttaatc aaacaactag aagctggttc agaatttcta gcaaagacat
    25321 gtttacatag caagattatt atcacggtgg atggtattcg gcttgtggaa acaaaagagt
    25381 tccacccagt gagcggaact ctactagatt agacaatacg tgtataagtg tgtattttag
    25441 ctaatctatg aatatttgag caaaactaca aaaggagaaa atcaatatgg tgacaaaatt
    25501 ttcagaagaa ataaaaatag taaggacgaa gtcttctgtt tatgtaaaca gcgtcttaga
    25561 cgttgcagat aaaaaagacc ccgcgtctgt tgcagcagtc gcagagcttt taaaggcgta
    25621 taagtcgtta acagaattgt aatcaactat ggtgacttat atacgtgact tgatcgagat
    25681 tgaccaaaac gatgtattga gcgtctgata gtctcagttc aataatattt ggattatcag
    25741 ggcactcatt tgcaacttgg cagaatgtgt gttgcttacc atttgtgaac atgattgtaa
    25801 tggtatcagt ttcattcgct agttgttcga taatatatcg tttcatacaa tcatctcctt
    25861 tctgctcata ttatagcagt taaggacaac tagagaaagg acagtatgca agcaaatcga
    25921 attttttacg gaaaagaaaa accaacaaac gcagtagcgg gcgattgctg gtttagaaat
    25981 aacgaattaa tgatactcaa gaacaattgg gaagctagcg taaacgtcaa cggtttcgtt
    26041 gtaaataacg cttaacaatt cgttaccatc ggcgaaaatc attttcgtaa cttcagcacc
    26101 tttgataggg atgacttctc ttccttctgc tgaaggtgtg ttgtacgata ctttttcacc
    26161 taacttaaat tggacatgaa aataattagg attcatgttt ttacctcctt tccgtaatga
    26221 tagctttatt atagcacgca ggaggaacta cacagataga aaggaagcac atgcgaccaa
    26281 aacgatatcc gtatcaaaaa aataaattaa ttatccagtc gaaaaacgca aagacaaaaa
    26341 ttgaattaga tagtggaaaa ataactatca gtgctgaaga aatcaaggtt ggtgatttag
    26401 caaacgaaat ttcaaaagtg caactaacta aagaaaaagt aaaattgccg atgtcttagg
    26461 tgtcagccta gacgaattta aataacaaaa aaagtccgac gggaatcgga ctctaaacaa
    26521 acttaattta cttaattata tcacgaaagg ggcgataaat ccatgcaatc gcaacttact
    26581 tacgatctac tcaaaaagca gatagcagaa gaactttttg atgagtttaa gaaactcata
    26641 caggaaaaag atttagcaaa tcagtgggtc aatcaaacaa cgctcgtcac tgaatacggc
    26701 tactcatggc aaactataaa acgcatggaa agctatgggc ttaaatcttt taaaaacggc
    26761 aaagacaaaa tgtactgtct tgctgatatt aacgacatta aacacttaat gaaacaataa
    26821 gcgctgggga gtgcaaaatg aacaaattag aaattttact acttacatca actatcatct
    26881 tagcaatcat cgctagagtg caacacgaag tcatcaaaaa acacaattcg ccagagaata
    26941 aacgcagaat ttttagggaa gtagctttag aaaacagtaa agggtggagc gataagcgct
    27001 ctagaggaga ggtagaaaaa tgccttttgt aaaacggttt actaaaaaaa gaacttgaaa
    27061 gagaagaggt ggattaatgc acatcgacga agttagaaat aacaagtttt accaattccc
    27121 tcaatggctt ctagaagaac cgtacagcaa cttgagttat cgagcgatgt taatgtatat
    27181 gcttcttttc gatcgcaggg gattatcgga ggaaaatcag tggtttgacg ataacggaaa
    27241 aatctttatg tattttacca atgaacagtt catgaaagcg ttgaaatgca gcgagccaag
    27301 tgtccttaaa tccaagaaag aattgcatca agcaggactt ctagatgagg ttcaacaagg
    27361 tgtaaataag ccaaatcgct tatatatcaa tggaactaaa aactttttag gtcaggaact
    27421 taaaactttt aagcatggaa ctaaaaactt tttaggtcaa gaccttaaaa gttttaaggg
    27481 aatcaatact aataatatca atactaatat atctaattat tatgatgata aagggactag
    27541 ttttttagaa gaaattgaaa atctttgggg gagacaattc aacggttacg aggtacaaca
    27601 aatcaataga tttttactag aggaacacat ctcaaatgac ttgctaaaaa aagctatcga
    27661 aatctctctt gctaacggtg caagaaacat gaattatgtc tcagtagtga ttaataactg
    27721 gattgacagc ggtataacaa cagtcgagca agttgatgct gctcaagaac ttagattaaa
    27781 gcaaaaatct aagactaata gcaaagatga agaggtatcg gaatatgata cttggtgacg
    27841 aagacgcact tgccaaaatc gctatatctt atcaacaaaa caccaagaaa gaagatgtcg
    27901 tctgtgaaaa acacaattgt agatacatca cagttctcaa gactggtttg acagtatgtc
    27961 cagattgtca cagagaagaa ttagaaaatc aaaacacttt gcacgtccaa aagcaatatg
    28021 agagagaact tgaaaacaag cgattgtact atctcaaaaa attatcaatt atggacaacg
    28081 agttggaaaa tgcatcattt gacaatttta gggctgatac ggctaaacac aaagaggtgc
    28141 tttcttgggc aaagacaatg gctaacgatt ggtttattgg cggcaaggga aacatcatca
    28201 tgactggcaa agcaggacga gggaaaagcc atttagctta tagcatcatt agggggctgt
    28261 ctgacaaaac taaaaaattg ggattacttg taaatgttac tgacttactg tcagagataa
    28321 agcgagactt tagcaaggaa gcattttgga tggataagct aaaagatgtc gattatctgg
    28381 tgctagatga tctaggtgcc gaaaaagtta gtgactggtc cacaagtatt atttacagct
    28441 tgcttaataa gcgtaccaat acaatcatta ctaccaatct gacacctgcc gaaataagaa
    28501 aaatttatgg agagagaatt gcgtcacgca ttagaaaagg ttgcgacaaa agacatatca
    28561 tggaatttga tggcatggag gacgaaagga tgaagctatg gaattgaaaa aaagaaataa
    28621 atttattgaa aacacagcaa acttgatact tcaaatggct gatgaaaaca aaaaagagaa
    28681 catcgctaat ctaccagatt acattagagg aatgtttgat ttatgtatag cgtttatgct
    28741 tgaagaggac aaagaaggaa ctggttacga ttcggtaatt gtaatgatag ctgaagaatc
    28801 ggagaggtca aaaaatgaga gagacgctaa ttagcttttt cggcatttcc gaagaacatg
    28861 cagccaaaat catggcgcta gaagaagatg tccgaacaaa gaaaattgaa gaattgagag
    28921 cgtggagaga ctgctcaaaa gtaacgtttt aggagatgtt taatatggtg gtgccactag
    28981 atgagttaga aaaaagaata ttttgttatt tgccgagagg aagtaacagg aaggttaatt
    29041 caaaggaaat ttgccgaaca tttgatatca aggacccgaa aaccttgaga gacattatcc
    29101 acgggatggt aatgaaaggc tatttgatag gttctagccg tcgaaaaggc ggagggtact
    29161 atctaattga caacgacgat gagcgtgcag aagctatcag ttccattgaa agtcaagtta
    29221 gaaaagaaca aaagcggata aacattttaa aaaatggaaa cttaaacgaa ttcgtagaaa
    29281 tagctaatga ggtgcgaaat ggtcgaaatt agaattaacg gagagacaat cacatttgat
    29341 agcaatttta gagatgcgtt tatctttaca attgactgcc ttagaggaag cgaagagcca
    29401 acactaaagc agacctatca agaatttaaa gactatacag atgaggactt gatggactac
    29461 atcgagacgg aatttgatgt aaaacctgaa ttaatcccaa accgcagaat tgatagtaaa
    29521 tggattttca aacaacatat tttggatgat taaacatggg aaaatcagat tttaaattta
    29581 gcgaagactg ggagagtaac tactttgagg tccaagcttt gctaggacaa gagatagaca
    29641 agctacaaaa tagagttgtc cagttagcga acgaaaacag aagattaaaa gccgaaaatt
    29701 ggcaactgaa acacagaaag aggaaatgat atgaacatca aagaaaaaat tgtagtgcta
    29761 agaaaaacta aagacggaag ttttttaaag agttttaaaa atagagatgc agtgcttgcc
    29821 tataataagg agtttacaaa tatcattcag gcggcatcat ttctaccaga agaagcttac
    29881 aacatgcaaa aagataaaat cgataattta gccgaaacgt ttggatgtga tgttgtagtt
    29941 gtcgaagcat cttatgacct gaaatttatt gatggtgagg atgttccaga gttaacaaaa
    30001 gagcaaaaaa ttgaaagtat ggtaaacgga atgtttgagc aggtttttgg aggtgaatag
    30061 agatggcgaa tgaactaagt gaaagacaga taacttctgg agttaataag cgaattgagg
    30121 aaaaacaaaa cgaaaacttt atcgttcctc ctaattatag tttgggaaat gctttaagta
    30181 atgcttacta tgaattaaaa aactcttcta gtggtaactt gttgagtcaa tgtactgatg
    30241 agagtattta tatttcgctc ttggatatgg tcgcacaagg gttgagccct gcaaagaaac
    30301 aatgttactt catcaagtac ggtgataaag ttcaattaag gcgctcgtac ttcggaacaa
    30361 tgaaagttgt taaagagcta aacgaagtta aagatatttg ggcaggagta atctttgaag
    30421 gagatgtttt caaatcagaa atcgttaatg gtcgtagaag atttgtaagt catgaatccg
    30481 attgggaaaa ccaagacaat ccaatcaaag gtgcttattg cattatcaag gatattaata
    30541 acgaagaata tctaaccatt atgactaaaa agcaaattga taaagcctgg tcaaaagcaa
    30601 aaacaaagaa cgtccaaatt gacttccctg accaaatggc aatgagaaca gtaatcaata
    30661 gagctgctaa attatttata aatacaagta atgatagctc gtattttgtc gaagccctaa
    30721 accgtaccac agaaaatgag tatgacaacg acagacaagt caaagatgtc accccacaag
    30781 aaacaaacaa tctagacgac ctaattggtc accagaacga aaaaatagac gcccctagca
    30841 atttaaaaga cgtaactgaa gatttacatt cagaaccaga aaaaacgctc acagacgaaa
    30901 ataaggctgt tttagaagat acctcttatc cagcagatga aattccggat tttgaccagg
    30961 aaactggcga aattaaagct agcgaaggta acctctttga taaccttggg gacttaatat
    31021 gattaaactt gtaaaaattg aaggatatta tattaatcca gaatatgttg ttggcgtttg
    31081 ggaaagatct gctttaaatt ttgacgattt agatgaaaaa gtagtggttg ttcaattcgt
    31141 tggagatagg gaaagggaag agtttgtctt ttacgataca cctatcgatg aagtgattaa
    31201 gaggttgcta gatgactagt ttagacttac tcggaaagga ctattacagc cgtgaatcag
    31261 ctatcaggta ctggtccatt agtcagtaca agcgttttag agagtgtgaa gcgagggcac
    31321 ttgctgaatt acgaggggat tggacagata ccagagataa cactgcgctg ctcgtcggga
    31381 actatgtcca ctcttacttt gagagtaaag cagcttattc caattttgtt gaagctaaca
    31441 aaactgccat gctctcaacc agaggagcga cgaaaggtca attgaaaaaa gattatgcag
    31501 ttgcaaacca aatgatagag gcgcttaaaa gtgatagcaa cttcacggcc atctatcaag
    31561 gggaaaaaga agcggcaatc acaggtttaa ttggtggtgt ggaattcaaa ggaaaaatcg
    31621 actgcctgaa tgttgatcgc ggctattttg tagacatcaa aacaacaaaa ggaccgattg
    31681 acgacacaat ttggaatgga gaagagcgtg tcagatggtt cgaggcatac ggatatatct
    31741 tgcagatggc tgcctacaaa accatgttgg aagctaagta taataaaccg ttcgagccga
    31801 ttatttatgc ggtaaccaag gaaacaccgc cagatacaag agccatcaga atccaaaatg
    31861 tagatgctat gcagatggaa ttagatagcc tagcacaaag tattaagcga ttagatgacg
    31921 ttaaaaaagg cctagaaaag cctaagcctt gcggaaaatg cgaatattgc agacaaaata
    31981 aattgtctgt aagagtagag attttttaat aacctattgc aaagtgaagc tcggcctttg
    32041 cagtaccaat attttccgag cgagaaagga aagttgaaat atcgtcaaat taacaggatt
    32101 gatgatatga agaattgcta cactcgtcct tgccaatgct cacacacaat tttagggcga
    32161 gtgtggattt taaaaaggtg aaaaatatgg aacaaatcaa aattacagga acaggaacag
    32221 cactaatttt agatagagta aaccgaatct ttgccatctc tggcggtttg actatgcaat
    32281 gggattttat tagtgatttt aaaaagattg acgacgaacc gtcacttgac gaagatggag
    32341 aactattcga agtagcttac gaccttgtgc tcgaagctaa acccaaaact aaaatcaatc
    32401 taacatcatc atattttgct aaagagcata agaaagacac agatgaaatc ataaaagtat
    32461 tctcgtttat cgaagataat aagaaaaata tctttgaaac ccttggcatt cgtggggtgc
    32521 ttgaatgagc aatctagtat tatcgctaga catttcaaca tctggaacgg gttgggcctt
    32581 atttaaaggc tcagacctta tccagagcgg tgtcttaaaa cacaaaagtg actcttattt
    32641 cgaacgtggt cgatatatgg ctagccaatt aaggttgatc cagtcacgag cactaaaaaa
    32701 atacgactgt agttttagca caatcgcagt tgaaaaaaac tcagttatgg gacctaacca
    32761 gcaatccatg cttaaaatcg gtattgtaac tggtatcatc ttaggacgat taatagctga
    32821 taatgttgtg tttgtcaatg tatcaacgtg gcgcaagcac tggaaattta gttataagga
    32881 tcgaagcaag aagtccatga aattacagtc aaaaacaaaa gtggagcaag aatttaataa
    32941 atcggtcaaa gatgacgaag cggatgctat tttgattggc tcatactatg tcaatcaagg
    33001 ctatcttgat ggattggaga cacatgacta ctactaaaaa gcgtgttgtg agagtttaca
    33061 acaaaggcat tacagctaca tacatggttt atgacaaaaa tctgtttaag gagcatgagt
    33121 ttgtaacaaa agttgaagcg aggcagttta ttagacggct agagttagct aatgataagc
    33181 gagtgacaga atattatttg agagaggtaa aagaatgata ccaaaattta gggcatttaa
    33241 taaaaagacc aaaaagatgt atagcattga tggctttaaa gcaagtgaac gcaaaatata
    33301 cagatgcagc ttcgctgatg atgagtttcg ctctggtcgc ttagagacgt ttcattttgt
    33361 cgaggataac cttgacgatt atattctcat gcaatccaca ggactcaaag acaaaaacgg
    33421 tgttgagatt ttcgatggag atgttgtcaa ttttatggtg ccaaatcaaa tgatttcagg
    33481 aacttatcag attagacaag caaaatcagg agagtggaga cttgataaca gagtccaagg
    33541 aagaccactt tatatctctg gaagttacca ctgtgaagtc gtcgggaacg tatgggagga
    33601 tggcgattta ctagaaagtg tggaagaatg aataagcgac aacgtaaaaa gaaaatattg
    33661 aatggattag acaaagaaga aagatatcat agaacacact gtcctgtatg tgacgaaaaa
    33721 ataggagtat ttgataatta tttcaatagc tacggttttt gttcagtttc ttgcgggtat
    33781 gaatactatg gactttagta catgtgaaag atgaggtgga agaatgacaa aagaagaatt
    33841 aattgaagaa atagaaggtg aaatcaatga cattgatctt ttgattgacg attatcaata
    33901 tgaaattgac gaattacaac gagatataga tgaattagat actcgaaaag atattttggt
    33961 tcgtaaatta cagaacctac tagaaagcgt ggaagaatga cagaagaaca gatgattgat
    34021 tgcttgcttt atgagttagc aaaaaaagac aaagcaatcg agaagaaaga catcattatc
    34081 gctgtactga aagtcatgct gtgtctcgtg tttgtttttg gcatcgcact acaaaaccac
    34141 cacaaaccgc aaatcactgg tttgcagtcg caattacgca ggacacaaaa gcagcttaaa
    34201 cgtgctagcg agcaaaacca gcagcagaca aaacggattg ctgaattgac aggaaatggg
    34261 ggatgagatg gatgataaca caacagttgt tttgacactg ctaatactat ttttgccttt
    34321 tattattgga agttttagag attaagtgag gtacaaaatg acagagcaag aaattaaaaa
    34381 tctgcttaat gacagcagaa aactagtatc agaagcaatt aacaagtgct atttagcgac
    34441 aagcaaaatt cctctatctg ttgataatag cttgattttt agtgcttacg attatattat
    34501 tcacgacatc ggtgacgttg ataaaatagc taaagaaata agtaaggcag gggaaaatga
    34561 acattaacaa caaatccata aaaactatta acaggatata cacatacggt gacagactag
    34621 aaatgtgcga aaacatgcaa tcatacgttg cagagagaga cgcgttaata aaagatttgc
    34681 tgagactaaa aaaacctatc gacagggata gggtatccgg acgattatat agagtgcaaa
    34741 ataatattag cgagcttatc ttaagagtga cagatgtatg gcgaggcagt gagcaagtca
    34801 ttgctgagca gtgtttttta aaggtattag aggagatgcg agatgaatat tgaagaagta
    34861 ttatacttac cagttaaacg agaaggttta agcaaaggtc cagatgagtt tgattgcaaa
    34921 catcgttaca gaacgcttga tggcgatgag gtagcatcat catttaaagc aaagtccaaa
    34981 gaagaattac tcgaccaacc aaaaccagtg gtgccacaat gtgctattga ttattatgaa
    35041 caatataagg aacttttatc agattttgag gaatggttcg gagattttta tgacagttat
    35101 tttttaaaag aattcccaaa aggtatcgag ttagcagaat ggctacatga taatgacaat
    35161 aaaacaaatc tacaacacga acttgcacta gctacattaa ttgtaaatgg ccctgatgct
    35221 gtcacagtag aaaaagaaaa gctatacact gttgagatac ctaatccgaa tataatcgga
    35281 aacgaacata ctgtacttat gaaaaatggg tttgggcaaa ttgtgatgtt cagggtttat
    35341 ggggatgact ggagaactga taaaggctat caaaacaccg aatcagaaat ccgaaaatat
    35401 tttgattggg cttggagaga gggattccag aaagaggtaa cagatgactg aagaattagg
    35461 agtattgctt gacaaggaag gaattgaata cgtaaaaatg gtatttgatg atggttttgt
    35521 ctggacggta cgcaacccac taaacgctgc gttgcttaaa cgtcaagaag cagagcagtt
    35581 cccacagttt acgtgggtgc cactgacaga tttataacct cacgcaagcg ctcaagagcc
    35641 tgcaatggct ctgtggggtt aggacctgga atcgtcctta aacttaccct agaaaccttc
    35701 aatcttttat cagccttgcg gattgtcaaa ggtccgtaag gtgtagaccg aaattaatta
    35761 aaaaaggatg tgataagaat ctccttaacc aaaaaaaacc aaatctaacg ctggttatcg
    35821 gtcactagct attattcaag gcgctaatac tgacatcgta cgcctgtgtc aaataatttt
    35881 atccataaat ctaaagcgat aatagtcgtg attatgcaag gcacttaaaa aatcttgtgc
    35941 aaaagacagt ctcgtgtgcc tggaccaact tcagcaaaaa aggaaagaga gggcttttct
    36001 ccaaaaaaaa caaaaagacg tccatgcgaa cgccttcatg attaaaaatt cctaacaata
    36061 ttatatcatg aatggagagt tcgatgggca acattccgac aacaaaagct aataattttt
    36121 tggaagaatt aaagactatc ccacatctca tagagaccct tgaaagagac gctaacttga
    36181 tgagtcgctc gctcgtcaag tctcctcaat ggtctgatat gaaggtatct ggcggggtaa
    36241 aacaatcaca ggaagacaaa aacataaaaa tgttacacat ggtcagctat tacagtgatc
    36301 agattgagcg tttaaaagac cgtcggcaag aaatggctaa tttgattgtg caaagcatgg
    36361 gcatttgtga gagccatgtt ttgttaacga cttatcttga ctgtgatgga gactatgaga
    36421 gagccagaga gcgtttaaac ataggtaatc gtaacaaata ctttatgttt gtcagaagag
    36481 gcaaggaaag tctagaattg atactaaaaa atactaatta gatacaaatt gatactacat
    36541 aatactaata accgtgttaa tattgtagta tatcaaaata acaagaagag ataaccattt
    36601 taccaaccga ctattttatt tagtcgccaa ctttaactac tatcgaactt gttattttgt
    36661 agcctgatgg cggtacagtg agttgagacg acaactgggt atgcaggttc gaatcctgcc
    36721 gtctcaatag tggttattta aaccactaga gcaatacagc gagagatgta gagcggagct
    36781 atagccgttt ttgtgtagat cttatggtat taatcgcgtt cgattcgtga tgggtctatc
    36841 agcgcccaga agaggcgcga ccccacatcc cttttaaggc agacacgttc tgatgtgtgg
    36901 atttttttga ttaaccgcaa gttcaaaacc aagggtcgca accttgcttg tggttagtag
    36961 ggacaacggt tgtaagtcgg ttcgattccg actgttcctg tgaaaagctc taagaagtct
    37021 acgggcctta ggggcgatga agttagagaa ttgcatatcg ctctaactca atatgcacta
    37081 gtcaccaatt gtggtggctt tttattatgt cagaaaggag gtagcccgct atttgggact
    37141 tacaacaaaa caagaattgt ttgtccaagg gatagtgtca gggctatctc aaagacaagc
    37201 gtatagacga gcttacaaat ccgaaaatat ggcggatgat accgtagata aaaaagcttc
    37261 tttgctcttc aataaggggg agataagggg gaggtatcgt gagctactca agcagttttc
    37321 taacatgtct ctctggtcaa gagaacaagc atttaacgag tacgaatggc taaaaaacaa
    37381 agcgagacaa gacattgaaa acgaaggtgt tagacaagca aactctaacg cttttttgtc
    37441 tgctcttgag gggatgaata atatagcatt taaagaactt gaactcgaag acaagaaact
    37501 tgtgaaagaa atcgagttgc tacaaatcaa gctcgatgct gaaaaaggct ctaagcctga
    37561 tactagctta atgagagctt tgttagatgc tgttgaggga gatgattaga atgcagattg
    37621 tattttcaaa caagcaaaaa gacatcatta gacgcccttt taactacgaa ttagaggtca
    37681 atgaggggac acccagaagt ggaaagacca ccgctgggca ttttcgttat gctaggtatc
    37741 tgattgaatc agaagatgaa aatcatcttg tgacggctta taatcaagaa caagcttatc
    37801 gtttgtttat tgatggagac ggtacgggct tgatgcacat atttgacggt aactgtgaaa
    37861 tcaaacacga tgaacgagga gatcacttgt taatcatgac gccaaaaggc aataaacgcg
    37921 tttattacaa aggtgggggt aaagttaata gcgttggtgc tattacaggt atgtcactcg
    37981 gttcagttgt tttttgtgag attaacttgc tacacatgga ttttatccaa gagtgtttta
    38041 ggcgtacttg ggcggcaaag ctgcggtatc atctagcaga ccttaacccg cctgcaccac
    38101 agcacccagt aataaaagac gtctttgatg tgcaaaacac gaggtggact cattggacca
    38161 tggatgacaa tccaatacta accgcagagc gtaaacaatc cattatcaat aacctgcgta
    38221 agaatccata tttgtacaaa cgagatgtac tcggacagcg ggttatgcct caaggggtta
    38281 tttatggtct ttttgacatg gaaaaaaatg ttttggatac tttgattggc gagccagtgg
    38341 agatgtattt ctgtgcagat gggggtcaat cagatgcgac ctctatgtcc tgtaacatca
    38401 taacaagggt tagagataat ggcaaaataa gttttagact taaccgagtg gctcactact
    38461 accacagcgg agctgacact ggtcaagtaa aagctatgtc aacctacgct ttagagttaa
    38521 aagtttttat ggactggtgc gttaaaaagt atcagatgcg ctatacagag gtgtttgtag
    38581 acccggcctg taaatccttg agagaggagc tgcacaagct aggagtattt actctggggg
    38641 ctccgaacaa ctctaaagat gtatctagca aagccaaagg tatcgaggta ggtattgagc
    38701 gtggccaaaa cattatctca gatggcgctt tttatctcgt taatcatagt gaggaagaat
    38761 atgaccacta ccacttttta aaagagatag ggctgtacag tcgtgacgac aatggcaaac
    38821 ctattgataa agataaccat gccatggacg agtttagata cagcgtcaac gtgtttgtgc
    38881 atcggtatta caactaaagg agttgcttaa atgggagtaa tccaaaaaat aaaaaatctt
    38941 tttacaagga gtaaatacgt gatgacaacg cagagtctta caaatataac tgatcatcct
    39001 aaaatagcta tcagtaagct agagtacgac cgtataacaa ccaatctaaa gtactataag
    39061 agtgattggg atagtgtttt atacttaaac acggatggcg agactaaaaa aagaaatctt
    39121 aaccatctgc caattgcacg gacagctgct aaaaagattg ctagccttgt ctttaatgag
    39181 caagcagaga ttaaggttga tgatgatgca gctaataaat ttattagtga gacactaaaa
    39241 aacgaccgtt ttaataaaaa cttcgagcgg tatctagaga gttgtttagc tttaggcgga
    39301 ttagctatga ggccttacgt tgatggcgat aaagttagag tagcatttgt tcaagcacct
    39361 gtttttttac cattgcagag taacacgcaa gacgtttcaa gcgctgctat tgttattaag
    39421 tccgttaaga taatcaatgg caaagaggtc tattatacct tgatagagtt ccacgagtgg
    39481 cgcagctctg atgattatgt tatctcaaat gagctatatc gctcagatga taaaactaag
    39541 gtaggtagcc gagtgccgtt atctgaggta tacgaggact taaaagacga ggctaaagtt
    39601 acagatgtga ctaggcctat ctttacttat ctcaagaccc ctggaatgaa taacaaggat
    39661 attaatagcc cacttgggtt atctatcttt gacaatgcta agacaacgat tgactttatc
    39721 aatacgacct atgacgaatt tatgtgggaa gttaagatgg ggcaacgccg ggttgctgtg
    39781 cctgagagca tgactgcttt aaaaatccat actgcagatg gtgatgttat tccgagacct
    39841 cggtttgagt ctgaccaaaa cgtttatatc cgtatgggtg gcagagattt agattcaacc
    39901 gcaatacagg acttaacaac acctattagg gcggatgatt atattaaggc tatcaacgag
    39961 gggttgtctt tgtttgagat gcaaataggt gtatctgctg gattatttag ttttgatggc
    40021 aagagcatga agactgcaac agaaatagtc tccgaaaact cagacaccta ccaaatgcgt
    40081 aacagtattg ttgctttagt agagcaatcg ctaaaagagt tagttatctc aatttttgag
    40141 attgctaaag cttatgattt gtaccaaggc gaatctccaa acatggataa catcagcatt
    40201 aaccttgatg atggtgtttt tacagaccga gacgctgagt tagactactg gataaaagtt
    40261 gttaatgctg gctttggcac tcgtgagatg gctatccaaa aagtgctaaa cgtgacagag
    40321 gaaaaagccc aagagatagc tgcagaaatt aatactggaa tcgttgacaa aatcaaccaa
    40381 cagcgtactg atacacattt atacggagag tgattagatg aaaaagaagc ctattaagtt
    40441 aaatgacgaa cagcttcttt tggaagctag tcaattatct gatatgtatc atcagctgac
    40501 tcttgattta tttgaccaag tgattgagag gataaaagcc agaggatcag cgagcttagc
    40561 tgataatcct tatctttggc aagctaataa gttacatgat gttggactgc ttaatgcaga
    40621 taacatcaag cttattgcaa agtattctgg cattgcggaa gctcaacttc gctatattat
    40681 caaaaatgaa ggatttaaaa tttataaaaa cacgtctgag cagttagaag aggctctagg
    40741 tagagagtct ggggtaaaca gtactattca agacgaccta tctaactatg ctagacaagc
    40801 tattgatgat gtgcataatt tgattaacac cactttgcca attagtgtca taggagctta
    40861 tcaagggata atccaagacg ccgttgcagg tgtggtgaca ggcttaaaaa cacctgacca
    40921 agctatcaat caaactgtga ttaaatggtt taaaaagggg ttttatggtt ttacagataa
    40981 agcagggaga aaatggagag cagactctta tgctcgtacc gttatcaata ccacgacttg
    41041 gcgagtcttt aatgaagcca aagaagcccc tgctagggag tttggcgttg ataccttcta
    41101 ttactcaaaa aaagctacag ctagagaaat gtgtgcgcct ttgcagcatc aaatagttac
    41161 cactggcgaa gcgagggaag aagaaggaat aaaaatctta gctttatctg attacaggca
    41221 tggagagcct gacggatgtt tgggaattaa ctgcaagcac actaaaacgc cgtttgttgt
    41281 cggcgtgaat agtaaaccag aattgccaga gcacctaaaa aatattactc ctgcacaagc
    41341 aaaagctaat gcgaatgcgc aagctaagca gagggcaatc gagagatcaa tacgtaagag
    41401 taaagagcta ttgcatgttg ctaagaaatt aggtgataga gatttgatta gtcaatatca
    41461 atcagatgtt agtagtaagc gagatgcact caattatctg ataaacaaca atgccttttt
    41521 acatcgcaat caagtcagag aaaagcgtta caataattct tataccaaaa ctcaaagtga
    41581 agtcgaagtt aggaaagaaa aagctaaatt agataaacgt agggatgttg aaagtgctat
    41641 aataggagta gaaactagtg aagggatacc gctaaaaata acaaagcatt tagccgaaag
    41701 ggcggtgctg agaaatatag cgcctattga tattgtcgat tctataagag aaccgttgaa
    41761 gatagctcct attaagtacg ataaccttga tagaccttcc caaaaataca ttggtaagcg
    41821 tgtatcaacg gtaataaacc ctatagacgg aaatattgtt acagtttatg ctactagcac
    41881 gagaatccgc aaaaaatatg gaggaaatcg atgaaattaa aaaatatttt aaatgatagt
    41941 caaatcgatt ttgtcaaaaa tgaacttccg ggattacctg tggatataga ggttacttcc
    42001 gaaaagtatg atgttttttg tgaaggtata gagacttact accagacaga aagctttgat
    42061 gaaaagtaca atataacagc taaaggtaaa ctagctgaga gtattattga tttactgact
    42121 gataaaggtt attggtgaca cgacttatta aatctagagc atttagctaa aggctaggtg
    42181 ctttttttgt acctaaaaac aggaggaaaa catgaataaa cgcatcaaga aaaaacgtaa
    42241 attggaaaca gctattgtgt tgcttatcgc agaaaatacc atgcaggctg aagctattaa
    42301 aaatcaagac cgacaaattg cagagttgag agcgattgtg caacaaaacg cccaagcaac
    42361 aaacaacgaa ttagcaacag ttacaggcgt atgttttgat aatcaagcag ctattgtaga
    42421 tattgcaatt gatgttaact acattaagca aaattacaag cggaagtggg gtaagaaata
    42481 atatggaata tacagaaaaa gctaaagaac tagcatcaca agaatttaca tgtctttctg
    42541 gtcgtgaaat caaaccagaa gactgctttg tagtctggtt ttgtaaaaca ctacaaaact
    42601 ggaaagcact tgtcagcaca aatcaaatta aaacaggtga aaaatgtggt gattatgcag
    42661 agatcacaca caatggcgat aaaaaagaga cttacgtaga tgtttatgcc aaagtctcga
    42721 atcaagcttt tgcagattag caagcggggt aaatgatatg gcattaaaac aacaagaaaa
    42781 agcatcattc aacggcctta catacatgcc agcagaggca gtaatggtaa aaatcccaaa
    42841 gtcagaatat gatctgttga ttgaaattaa aaacaaattt ttaaagtcgt agcaatatgg
    42901 ctttttattg tgcctttatc cgcaggcgtt aaagaacgga aatcagcgac ctatcgcttt
    42961 tataggagga aatacacatg gaaaatgtga tgaatgaaaa cgtcgaccaa gagactactg
    43021 gcttggaaaa taatcaacaa gaagataaag catttacaca agatgatctc aaccgagtgg
    43081 gaactaaaga gcattctaaa ggctataaca aagcagttaa agacttaggt tttgatgacg
    43141 tggaatcagc caaagatgca ctaaaagcct tccaagagtg gaaagagtcg caaaaaagcg
    43201 agtcagagaa acaaacagaa attttgaatg ctaaagacaa agagcttgag gaagctcggg
    43261 caatcaacaa ggctcttaat gcaaagctag cagcaatgtc tttaggtgtg aaagctgaat
    43321 ctattgacga tgtgattgca ctatctgagc gtcttgtcac tgacgagaca agcatagagg
    43381 atgcaatcaa aacggttttg ggtaagtatc ctcactttgg ccaaacaaag gataaagctc
    43441 ctaaaatcac agtggcaggc aacccgtctg cggataacgg acaaggttcg gtgtctaagg
    43501 aagactttgc aaagatgtct taccaagaaa aactggacct aaaactaaaa aataagaatc
    43561 tttatgacca attgaaagga aattaaaaat ggcagtagga acaactaaaa tggcacaaat
    43621 gctagaccca gaggttctag cggatatgat tgacgcagaa gtaggaaaag cgattcgctt
    43681 tgctcctctt gcagaagtag atacaacctt agaagggcaa ccaggtacca cactaacagt
    43741 acctaagtgg gattacatcg gtgacgcgga agacgtggcc gaaggagaag ctatcccaat
    43801 gactcagctt ggatttaaaa agaccacaat gaaaatcaaa aaagctggta aaggtgtaga
    43861 aattactgac gaagctattt tatctggata cggcgatcca gtaggccagg cagccaagca
    43921 aatcgtagaa gctattgacc acaaagttga tgcggatgta ttgactgccc ttagtggttc
    43981 tactcaaacc gttagcgctc ctgcttctgt tgagggagta tctcaagcgc tcgacatctt
    44041 taatgatgag gacgatgctg agacagttat tgtcatgaac ccagcggatg catctactct
    44101 acgtttaaat gctgcgaagg agtggctagg tgctaccgaa gttggggcaa atcgaattgt
    44161 atctggcgtg tatggcgaag ttttaggggt acaaattgtg cgctctcgta agtgtcctaa
    44221 agggactgct tacatggtcc gaaaaggagc tttgcgcatt atgcttaagc gtaatacaat
    44281 ggttgagaca gaccgagata tcacaaaagc aattaatcaa attgtagcga ataagcatta
    44341 tggtgtttat ctttataaag ccgaaaaaac agttaaaatc acaatcacag aggccaagaa
    44401 gaaagagaaa aactttgaga tgtaggggtg gcatatgaac aaatataaag caatggtagg
    44461 ttttacaagc aaagctgaag gggtctcatg ttcaaagggg caagttgttg aattagacga
    44521 agcaattgct aatcagatta atgataaatc ctttgaaaaa tacgctgatt atgcaccctt
    44581 cttggagctt gtcgaacaga aaaaacaaac agttgctgat atcaaaaaag ctttagaaga
    44641 aaaaggtatt gattacccgt ccaaggctac caaagaggag ttgttagcgt tactagcgga
    44701 atagatagcc gctttattct ggggaggtgg ttaacatcac ttttttgaca caaaaagaat
    44761 ttgtagactt aggttttgat gaggtagaag attttgaaaa aatggaaaaa cgtgctggcc
    44821 acattatcaa tctttactgt cgtaatcgct atgattacaa agattttaaa aatgaaatag
    44881 ctctagtaca aaaggctgtg aagcgagcaa tcgcttatca gatagcatac ctaaacgact
    44941 caggagtgat gacggcagag gataagcaat cctttgctgg aatatctcta gggcggacaa
    45001 gcattagcta tactgtcggt cacggccaaa gcagtcaaca aaaaactttg gcggacaggt
    45061 ttaatctttg tctggatgct gaaaatgagc tactggctgt cggattagga tatacaggta
    45121 ttagctatga tcgataaaag attgcttatt gacaaactgc aggtaaaact tgtcaaagag
    45181 aaaggagact atggagggtt tgtctatgac gaacctttca tagtctcccc ggttagattt
    45241 gaccgaaatc tctcgaccgc aggtaaagat aatgcaaggc aagagactaa gccgtcagtt
    45301 atctttattt atcctaaata ttgcaaggtt gcagctgata ggacgtgggt tgatgctgca
    45361 gtacttgatg gtgatacaga gtacattgtt gacaaagtga taccagtcta ccacccacta
    45421 acaaacaagg ttttttgtta cgaagtggag gtcatttaat ggcgaagata gtggtagagt
    45481 tgggaggcat taaacgtaaa gtatctcctc aggcgcttgc tagagggaaa ctcgtcatga
    45541 acaaccaagt catgatgtcc atgaatccct atgtaccata cagggatggc gctcttaggg
    45601 gcagctcgag agctaatagt atgggggtaa tgtggagcgg gccgcatgca agggctcaat
    45661 tttatggtgg tgcatacaac aagtacaagt cctttaaatt taaaaagtac acaacaccag
    45721 ggacaggtaa acgatgggat aaacgtgcac tagccaatgc tacaatcgtc aaggattggg
    45781 aaaaatcctt actgagagga atgggattta aatgacaaat gactttgcaa ctgttttgag
    45841 gcaatttata gaaggcttag atctagggtt aaagcctaga cttgactatc taacaagaca
    45901 agaagatcta gctatctacc caatgccagg tggtaaggtt aatagtgagt acatggatgg
    45961 tactcgagag ataagcctac cttttgaaat tgcaatcaaa actaaaaatc aggagttagc
    46021 aaacaatgtg atgtggacta ttaacagtgc tttgtctaac tttgacttaa aattaccaag
    46081 tcttaataat tcgtacacat ttactagcct tgatgtcgaa aagccatttt taaatgactt
    46141 aagcgaccaa ggcttttata tttatgtgct ggatattacc gcacacctag aaatagaaag
    46201 gaaaaactaa tggcaagaca aaagaacgcc ttacgcgggc attttatcgc accatacaaa
    46261 acaggaatat cagaaacaga ggttactgat gagaccgtta tggaattagc gaaatggatt
    46321 aaggatattt ccgatgatac cgacgaaaag acagaagatg aggcttacta tgacggagac
    46381 gggacagaag aaacaaccgt tgtaggagtt aagggagcgt atactttcga ggggacttat
    46441 gacccagaag acaaagccca agctcacatc gcaagtctta agtataagct gggagatgag
    46501 cgaaaagtat ggcatttaat tgtagcagca gacggtaaaa aacaatggtt gggcgtagca
    46561 acagtaacag aaattattgc tgggtctgga gctgcggcag attttgaagc ttttggttgt
    46621 aaggttacct acaatacttt accaaaagaa agttcagaaa tgacgctaaa gaaaaaagag
    46681 aagaatttcg agatgtaata gagccagctt acgctggctc ttatttaata aggagatgac
    46741 atgtcaatga ttaatattga tcttaagcgt acaggcttcc ctgtaaaaat tggagtggtt
    46801 gaactttggt tcgacaccag ccaggagcgg ctacttgagt tttttgatat agagactgag
    46861 gtttcacgtc gtttgaacga gtacgaaaaa cagattattg aagcaaatct caataagaga
    46921 attgaagacg aaggggtcac caaagaagtc gctaagggcg ctttagattt agaaacaaaa
    46981 tacctagaaa ttaattacga cctgctgttt ggagacggaa cttttaataa gctttatgaa
    47041 acttaccctg acaaagaggc gctcgagagc actcttgaag ttgtctctag ggaaatcgag
    47101 gtcaaactca aagaattagc tatcgaaaga gaaaaaatcg ttaagcagaa aatgaaaaag
    47161 tacaaaaagg agtagcctat gaaactaaac gatccattag tagtgtcgtt tgagtttaga
    47221 ggcgaaacct atcctattga tttgagtttt aacaaggttt tagatgtctt tgatgttatt
    47281 gatgatgatt ttttaaacga gacggaaaag tgtttcttgt gtcttgatat tttattagac
    47341 agaacggaat tgccttttac ttacgctgtg gatctttgga tttatatcaa aaaaaattat
    47401 atcgatacgg aaaggcctga gaaacctcag ctcgacatca aagggaatcc aatgcctata
    47461 gtaaaagaaa aagaggataa caaaaaagtc attgatttta gtctagatgc tgaatttatc
    47521 tacgcaagtt ttaggcaagc ctatcaaatc aatcttttaa aagtgcaaaa caagttatct
    47581 tggattgaat tcaaggctct tttaaatgca ctgcctgaag atactattat gcaacgtatt
    47641 atagctatta ggcagtggga agatgatggc aaaggcgata aggactatag agataacatg
    47701 cgtaagttaa aggctaagta cagtttagat gacggagagg agggagacga tggcagctga
    47761 tggtaaggta acgatacttg ttgacgttga tggtaagcag gtaaaggtgc tcaataatga
    47821 gttagataaa gttgccaagc acggtgataa aggcagctcc tctcttaaaa aatttgcggt
    47881 tggtgcagga gtctttaaat tagcttcggc tgcagttgat ttggttagcc aatctcttgg
    47941 taaggccatc acaagatttg acactctgga aaaatatcca agagtcatga aagctatggg
    48001 gcatagtgct gaggatgttg caaggtcaac agataagctg gctaacggaa ttgatgggct
    48061 accaacaact ctagacgagg ttgtcggaac tgctcaacgg ttgacctcta tcacgaaaga
    48121 tatcaacaaa tcaacaaatc tcacgctggc tttaaataac gccttcttag cttctggggc
    48181 ttcctctgaa gctgcaagcc gaggattaga acaatatgct caaatgttat cagctggcaa
    48241 ggttgatatg caagcttgga aaactcttca agaaacaatg ccttatgcct tgcaacagac
    48301 tgcggaagcg tttggcttcg cgggggcctc agcccaaaag gatttttatg aggcattaaa
    48361 aaacggccaa ataacattcg accaattttc taataaactc atcgagctga atgatggtgt
    48421 cggtggtttt gcagagcttg ccaaagaaaa cagcaaaggg attgaaacct cttttaacaa
    48481 tattaagaat gccattgcaa aaggtgtagc caatagcatt aaggctttag atgatttatc
    48541 taaggccgca acaggcaaga gtatagctga tcattttgat agtttgaaag ttgttatcaa
    48601 tgcctctttt agcgccatca atgcaagtat taaagctagt acaccgctat ttaaactatt
    48661 gtttagtgtt attggggctg gaatatcagt cgtcaaagct ctgtcgccag ccctggttgg
    48721 tgtagcatct ggtctagctg ccatgagggc agttaatgag accataacaa tgattaaagc
    48781 gctaaataga gcttgggtta tggcatctgc atcaatgagt attggggcaa caaccattaa
    48841 gactgtgact gcggtacaag cggtaagtac cacgatgact aaagctgata tggtcgcaag
    48901 gctgtctcag ttaggtgtct taaaagccag tacagttatt tatggcgtta tgacaggagc
    48961 tattagccta tctactgctg caaccatagc cagtaccgct gcggtaactg ctcttaaagc
    49021 ggcgcttgtg gctttaacag gtccagttgg ctgggtagta ggtgcaattg gtgcgttagt
    49081 tgctgtcggt gtaagtttgt ggtcatggct aactaaagag tcagacgaga ccaaaaaact
    49141 gaaaaaagag caggaggggc tagtcgaaag caacaaacag ctaagagatt ctgtccgcga
    49201 gggcgttcaa gagcgtaaga agagtcttga gtcggtcaaa gagagcactg ctgctcatca
    49261 aaaattagct gacgaaatca ttaagttagc cgccaaagaa aacaaaactg caggcgaaaa
    49321 acaaaactta aaaaataaga ttgatcagct taatgggtct attgatggct taaacttagc
    49381 ctatgacaaa aactccaatt ctctttctca caatgcagat caaattaagt cacgcattag
    49441 tgccatggaa gcagaaagca catggcagac agcacaacaa aacctgttaa atattgaaca
    49501 gaaacgtagt gaggttagta aaaagctagc tgaaaatgcc gagctacgta aaaagtggaa
    49561 tgaagaagct aacgtctctg attccgtccg aaaagaaaag attgccgaac tcacagaaga
    49621 agagggcaag cttaaaaaca tgcagactca actgcaggag gaatataaca agacatcagc
    49681 tactcaacaa gctgctgcag atgctatggc tgccgctgaa gaatcaggat cagcaagaca
    49741 ggttttggcc tatgaaaata tgtcggaggc tcaacgaact gccatagaca atatgcgcac
    49801 taagtattcc gagctattag agacaacgac atctatattt gatgctatcg aacaaaagac
    49861 tgctctgtca gtggagcaaa tgaatgccaa ccttgagcag aacaggttag ctactgaaca
    49921 gtgggctact aatttggaga ttttggctca gcgtggtgtg gatcaaggta ttttggagca
    49981 actaaggcgc atgggtcctg aaggagccac acagacgcaa gtttttgtgg atgcaacaga
    50041 cgcagaatta gcacctttgc aggaaaactt tagagcagcc acagaaaccg ctaaaaatgc
    50101 gatggggagc gttttagact cagcaggcgt ggaaatgcca gaaaaagtca aagggatggt
    50161 cactaatgtt tctacgggat tacaggctga actgcaagct gctaactttg ctcaacttgg
    50221 ccaagaaatc cctaatgggg tttctcaagg tataagccaa gggacaggta aagcaagtga
    50281 cgcaagtacc aaaatggggc aagaagtcaa acgctctttc caaggagagc ttggtattca
    50341 ctcgccatct agagtgttta ctgagtatgg tggccatatc acagatggtt tgagcaatgg
    50401 cgtgacaaat gggacttcaa aagttatgca aaccatgcag agcatggctc aacagatgtc
    50461 tcaaaaagga cagcagattg ttaatgacat gcgtaccaaa tcaaaccaaa taacagatgc
    50521 ctttagcaca atgagtggtc caatgcactc tcacggtgtt aatgccatgc aaggtttggc
    50581 caatggtatt tatgcagggt caggggtggc tttagcagca gctcaaagta ttgcagcgcg
    50641 tatcaccgca acaatacaga gtgccctaga tatccactcg ccatctcgtg ttatgagaga
    50701 tgaggttgga cgttttatcc cccaaggtat cgctgttggt attgacgcgg atagaaaagt
    50761 cattgactca tctatgcaaa agctaaaaga gtcaatgacg attaatgcaa ccccagaact
    50821 agcctctgga tttagcggag gagttgctgg tgttgctaat cagaccacaa ataactcaaa
    50881 taacagtttc acccttaatg tcaaggttga tgaatccgac ggtaatagcc gcgagaaata
    50941 tcaccgctta ttcagagaat ttagctggta tattcaacaa caacaaggaa ggttaggtga
    51001 tgttaaatga cagcttttat caagtttgat ggtaaaaaat cttcagattt tggtttgaga
    51061 attattaatg acgttgagca tgactcgacc ttttacgatg ttgaccaagt taaagtacct
    51121 ggtcgtgatg gggttgtttt aaagaataac caaaggctcg aggctgtcga acgctcttat
    51181 cctttgcgtt tgtacagcaa gaggagattg acagaagcag agactgacat aagtaattgg
    51241 cttaatgtaa caggctggaa agatttagag ttatcttggg aacctgacta tgtctataaa
    51301 gcaacacata tcactccatt tagtataaaa gaggtcttac gtaactttgg tagattaaag
    51361 gttaatttct taatccaccc tgtcaaatat ttaaaaacag gtaagcaaga ggtgtctctt
    51421 gttaatggtg gcactttaca aaatcctggt aatgtgcaat ctaaacctat cttaaaaata
    51481 aaaggcacag gcaatggcgt cttgaccatt aatggctttg aaactggact tgaaaatgtg
    51541 cagggtgagc ttcttataga tatggagagg cgtctagtct ataaagacgt cttgtctgct
    51601 tgggataaca tcgtgcggac agaacgccac cgcatgccac tatttgatat tggtcaaaac
    51661 aaaatctcat ggactggtaa ctttactatc accgcagtgc caaactgggg ggtcaaagta
    51721 tgataccagt tttgtatgag gctagggaga ccaaatttag gacttttggc ctcggtgaaa
    51781 ttgcggatgc ttatgaggtt aaagccactc gtgagcgcaa tggtaattac tcactgtata
    51841 tcaaataccc attagatggt atatttgctt ccacctttaa agaggagatg aagattaagt
    51901 ctgacgctgg tcgtagaacc aagtggcaga cattcgaaat caatcgtgtg ctacggaata
    51961 gtaaagacaa catcgagatt tttgcgcgtc atatctctat gcgcacacag gatattgctt
    52021 taaaaccgtt tgtaaacggt gcgagcgtag gagccgaatc agctttagaa atctggaagg
    52081 aaaaccttgt cggtgatgat acttttgatg ttaaaagcga cattctaacg cttggcagct
    52141 ttaattggga aattgacaag gtcggtaatg ctagaagtgc tctaggaggt gttgctggtt
    52201 ctatcctaga tgtttacggt ggtgagtatg aatttgacaa tcgcaccatc atcttacata
    52261 agcaaatggg tcgtaaagct cctactgtgt tggagtatgg tcgcaatatt gtcagcgtag
    52321 aggaggagcg attgctagac ggcaattaca cctctattta tccatttgtt agatatacac
    52381 cacagtcaaa accgcaagag gaagcctctg gtaagccgca tgtaggcgag caagaacagc
    52441 ctgaagaaca actggtgaca ctgcccgagt ttatcctaga tggtcagtat cttgacttat
    52501 atgcccaacg cagaatccaa atggttgatt tatctagtca ttttaatgat gacaaaggta
    52561 aaaaagagcc aacagtcgaa gaaattagaa agctggctca gaaatacctt aaagataata
    52621 acgttggtgc accaaaagtc agtatcgagg ttgattatat tgacttgtca caaacacttg
    52681 actatcaaga ttttagagtc atggaagagg ttgagctttg cgatattgta ccactctact
    52741 atccaaaatt tggcatcacg actgagtctg aaaaagttgt ggagattgtc tatgacgtct
    52801 atacagatag taatcacacc atcaagttag gcacgattgg tcagtccatc tctaaaagct
    52861 taacaggtgg ggtctcagag cgtatcaatg cgttggaaaa taatcaaaag gttattacta
    52921 acaaccaaaa acagtttgag cttaatctgc ctaaatactt aaacgacctc aatggtaacc
    52981 gtgtctggta cgaaaaacca gatgacaaca tcgagcataa gataggcgac tactggtttg
    53041 agaaaaatgg gaagtatcaa cgcacttgga tttgggatgg caatcaatgg gttaaagtac
    53101 tagatacaga ggatttaaac cctaaccagc gcgcttttga tgaggcaatg gccgaaatcg
    53161 aaaaagccaa aaaagcgcaa gaagaaatca accagcgtac tgacaaagag cttgaagagt
    53221 ttagagacac tctcaaaaac ctagctttgc cagaagaagc cattaagaaa atcaccgagg
    53281 ctatcaaagt tgatgacatc ccatctatta aacagtcgtt tgacgacctc aaaaataagg
    53341 ttagtgagac gagcgaagaa tctcgtttaa ctgccgaaat tttagggaac aacggcaaga
    53401 ctcgttacaa caaaaatttg ctggttggcg accctaaccg caccaaaacc tatgatgagg
    53461 attttattga ggttgaagcc aatgatggtg gatttaagcg tggcgagact tataccatca
    53521 gctttagtca gacttgcgac ttactcaaaa aagtagctat cacgctgaca caggttaata
    53581 ataagggtct caaactggta ctgacaccaa ccaaggcaaa aatggagccg cagaccttta
    53641 acctgaccaa agataaagag gtcatcagcg tttatccttt gagctacacg gctgttttaa
    53701 caggcgaatg gtataaatct aagcaagtag atttaaatgc gtcagaggta caaaatttag
    53761 ctctggagat ggcttataga gatgtggcag atgccagagg tgcaaccatt atcggcaaac
    53821 agtccgataa accaaaaatt attttagacg gaaggaggga cagatgacgc tagaagaaag
    53881 aataccaatt aaagtcttat ttgaccgcaa agatgccgcc gagtggcaaa gattaaaccc
    53941 tgttattgat gatggcgaac tagttgttga gctagacact cacaggctaa aggtcggaga
    54001 tggtaaattg aactataatg acttgcctta ctatgaaggt ccacgaggag aatctattac
    54061 taaggtacag ctatccgaaa atggagactt atctgtatgg attggggaaa aagagactaa
    54121 actcggtaac atcgaaggtc aaaaaggtga taaagggaca agtattactg acatcacaaa
    54181 agatggtgag acaatgacaa tcaaactatc agacgacact caaaaaacct ttagtatccc
    54241 taatggccaa aaaggcgata gaggaaaagg agtagagagt gctcgagttg atgggtcagg
    54301 tcacttaagg ctaaaaatcg aggaagagca agaaaaagac cttggtaatg ttaaagggga
    54361 taaaggtgat agtattacca tcacagacca acagcgtgtc tcagagggcg tacagttaac
    54421 ctttagcgac aaaaccaaag tagtcatccc aaaaggtgaa aaaggtgata ctggtgatgt
    54481 caacggcatc aatctggatg actatgtcaa aaagtcagat ctaaaaaccg tgggggcgcc
    54541 agatgttaaa gctattaatg actttttagg gctatctcaa aaggtattta cgagcaaata
    54601 tagctactct gatagtcttt taaaaagcta tagtagccct agttactctg ctagctggta
    54661 tgtcaacgaa tcaaccaaag acgtcaatgt caaagacaaa gtattgatta ccataagtaa
    54721 cacgacaacc cgaatggata attacttgga ggtatcggtt acttatgttg gttctaacta
    54781 cgtgactgct acctcaacag ggaagttgtt aaccactcct ggcgaagtta atgtcctaac
    54841 gaaagaacaa atcgcagctg cttatgctaa aaaagagcat aagcatgaga ttgatgatat
    54901 tgatggctta caaggcactt tgaatcgcaa agcggatgtt atacattatc atcgcataga
    54961 tgacattgca ggactccagc aagcctttaa tgacaggccg acaatggagc gtatatcaca
    55021 aactctagca acagcactgg aagatgtcgt cagacaaggt caattatctg ggtatgttag
    55081 gtcagcggat atccaaaacc aacttaacaa cgttggtaaa gtaaaagacg aggtgacagg
    55141 acagtatctg tctgttcgtg tggtcgataa cggtcaagta ccaagagaca caagtggcat
    55201 gattatcttt gagagggctt gatgatggat ttatcagata aatatcttat gtcaatagtc
    55261 gatggcagga taagcagttt tacgacagga gatagtcttg tagtcaacaa ttattacatc
    55321 gactataatg acacctataa atttgataac gaggatttaa cattttatta tgcgacgtca
    55381 agccacgagg caattgctta taaagctaat cgcaataact acaaagtgct caatccacaa
    55441 aataaagcag ttaatagtct ttatagtgct attacggtta atggccaaca gattgcgaaa
    55501 atagtaagca acggaaaaat catttggcaa aaagataaaa tgacatttct tgatttgtca
    55561 gttggtcgtc gtgtacaggt ctcttttaac ggcaggacta tcacgattgt ttttggcgct
    55621 acgcctaaca gcaaagattt tgagcaattt tttgcagcat ctaataaaga tttgttactg
    55681 actatagata atcagtcata tgatgttgtt gcagctgagt atgtcaagtt tgcaagtctt
    55741 gttaaattta cggtaacaca atttagcagc tataaaaata caacggcgac taatgtaacg
    55801 gtagctgtca aaacagacga tacagcacaa agtcaagtgt tgctttttga cggtcgtcct
    55861 acaacaacat ttacaaagct tgatttgtat aagacgtata tctttgagag accaggctat
    55921 ccacccgaga cgagagaggc gactgccctt gattttggct atttaaaact aggtgacggt
    55981 catttactta ttaataagtc agacactgga ctctatatta agtcttatgg tacagctttt
    56041 gacgtcaaga tttacggtaa aaattaggag gtaacaattg agcagagacc caacacttat
    56101 tttagacgag tcaaacctcg tcatcggtaa agatggacgt gtgcattaca cgtttacggc
    56161 tagtcaagac aatcaaaaag tcaggctaaa aagcaactgc ttaggcacag cgcattttaa
    56221 ccagctgatg attgagcgag gagaccaagc gacggactat gtggcccctg tcgttgtgga
    56281 gggcagcggg cagtcgacag gcgtctttaa aagccttgag gagatgctta gtggcttaca
    56341 gtctattaat ttagagctga cagacaccga aaattccaaa ctttggtcaa aaatcaagct
    56401 gacgacaaac ggtatgttac gcgagtatca ccgtgataac atcacaacag agattgttga
    56461 gagcgcagat ggtatcgcta cacgtatcag cgaggagaca gctggtaaat tagcacttat
    56521 caatgacacc gtctcaggca tcagacgtga gtatcaagat gctgatagga agttatcgtc
    56581 aagctatcaa gctggtatcg atggattaaa agctaccatg gctagcgata aacttggctt
    56641 acaagctgag attaaagcat ctgcacaagg actatcgcaa aagtatgatg acgagctgcg
    56701 caagttatcc gctaaaataa ccacgacatc tagcggcact acagaggcct atgaaaataa
    56761 actcgacggc ttacgagctg agtttacccg ttcaaatcaa ggcttgcgtg tggagctaga
    56821 gtctcaaatt agtgggttaa gagcaacaca gcaatccaca gctagtcaaa tctcacaaga
    56881 gattagagat aggactgggg cagttagtcg cgtgcaacag agcttggaaa gttaccagcg
    56941 gcgattgcag tccgcagagg ataattactc tagcttacaa cagacggtta acggcttaca
    57001 aagtgatgtg gggtccccga ctggtaaaat ccaatcacgc cttactcaac tagcaggaca
    57061 aattgagcag cgggttacta gagatggtgt catgagtatt attagtggtg ctggagacag
    57121 tatcaagcta gctatccaaa aggctggtgg tattgatgct aagatgtccg caagtgagat
    57181 tatctcagca atcaacctaa atggctacgg tgtccgcata tctggcgagc gtatcgcttt
    57241 agacggtaat acgacagtta acggcacctt tactacaaag attgccgagg ctatcaaaat
    57301 cagggctgat cagattatcg caggcacgat tgacgctgct aagattagag tgatcaatct
    57361 caatgctagc agcatcgttg gtttagatgc caattttatc aaagccaaaa tcgagcatac
    57421 gattacaagt ttgcttgagg gtaaagttat cagagctcgc aacggagcga tgatgattga
    57481 tttaaactca gctaaaatgg attttaatag caatgccacc attaacttta acaacaagga
    57541 caatgcgctt gtacgtaaag acggtaccca cacagccttt gtgcatttta gcaatgccac
    57601 gccaaaaaat tatacaggta gtgcgcttta cgcctcgatc gggataacct cgtcaggtga
    57661 cgggattaac agcgcgtcga gtggtcgttt ttgcggtgct aggtttttta ggtacgcacg
    57721 aggttacgag cacacagcgc ttgtcgatca ggccgaaatt tacggtgatg atattgtctt
    57781 tagcgacgat tttaacatcg atcgtggctt taagatgcgg cctagcctaa tgccaaaaat
    57841 ggtcgatctc aataagatgt atcaggccat tttagccctc ggccgctgct ggttacatgc
    57901 taataacaca gcgtggacat ttaactcaga cactactagt gcaatcatca gagagtataa
    57961 ctcatatatt aatggattat aggagacaac atggatttaa cagtacaaaa caaagactta
    58021 aatacgctct atagcgtact agataaaatt aaggttacta atatgcgtgt caatcgcggc
    58081 cgcgctaagc tgcttgccaa agtagaggct aagctcagag agtatgccaa agacgaggtt
    58141 gatattattg atcaatacgc tgctaaaaac gacaaaggta agtggttagt tgatgacaaa
    58201 ggcaatgcta aattagccga cgtcacaaaa gtaactgagc ttaacgactg tttagacgag
    58261 ttagccaacg agcctatcgt catcaaaggc catgagtact ctaagcgatt tatcgacttt
    58321 ttggagtatc tggccgaggc cgaagatgag tttacagggg ccgaaattgt actgattgac
    58381 aacattttgg aacaatacga agaaagtaaa ggagaataat tatggcacgt aattggaaag
    58441 taacaggaaa atatcctcaa tttgacagca caggagcagt cgcaagtaca cacgttatta
    58501 tcgcagcaga agacggagct gttattccgc agcttgtcaa aaaagattta acaggaacaa
    58561 acgacaaaga tatcatcaca gcagtcctcg aagagtttaa aaaatcagag tatgtcgaaa
    58621 tcgctatggg cgaagccgtc aaaaaagtgg atgacttgga aaaaatctcg caggaaaccg
    58681 ctaagacagc aaaaacagct caatcagcag ctggcctagc taaagtatcc gcagagcgta
    58741 cgcaaaagat gattaatttg cagactatcc acgttttgac atccagcgat aaagtagagc
    58801 ctgacatcta caaaggcatg ttagagctaa tcgagccagc caaaaaaggc gagtatcaag
    58861 cttatgatgt ctttactatt gtcgacgagt cgcacgaaga gcaagcagga gaaggtaacc
    58921 ttgtctttgt gcacgtcaac gagccattta cttatgacaa acagacgctt aagaagctag
    58981 aggaagagga taaagttaca gttatcaagt acgccgattt ggttaagcag gattagaggt
    59041 ggttagatgc acgaaatctt aattcaaatc aaagagttag caggggcagt aagcgccctt
    59101 gccctcattg gtggtgctat gatttggatt tacaaaaagc ttgtcatcga accagacagt
    59161 cgcttggctg aacgattaca aacagaaaac aacaaattat tgacagacac ggtaaatcct
    59221 ctgactgatg ccatcaaaga cctaaattat aatctcaaca ctgctacaaa agagcgtgct
    59281 gagatgcgca aagacatcga ggtgcacgaa gggagacttg atgcccacga cattcgacta
    59341 actgttctag agaccaaaga aataaaggag aaataaaatg caagaaatta ctaatattat
    59401 tacaggatca tcactatcaa ttttaactat tttggcaggt attgtggtta agcttgttaa
    59461 agattatcta ctaaaaaaag gtggcgaaaa agcggttaaa atcgctgaaa ttgtcgctcg
    59521 taatgctgtt gaagccgtcg aacaaatcgc ttatgacaaa gacatcaaag gtatcgaaaa
    59581 actaacagaa gctaaagttg cggttcgtga cgaattatca aagcataatg tctatttgtc
    59641 tgataaacaa atggaagtct ttatcgaagc agctgtcaaa cgcatgaatg acaattggaa
    59701 aggtcagtga tatgacagta gatactgaaa aagccattgc gtggatgggg tcgaaagaag
    59761 gtcgtgtcag ctattccatg acctatcgca acggtccaaa tagctacgat tgttctagct
    59821 ctgtttgtag cgctttaatc tacgcaggag ctagtaatcc tggttggcta ttaaacactg
    59881 agtacatgca cgactggcta gttaaaaatg gctacacact gatagccgaa aatgatggct
    59941 gggatagtca acgtggcgat atttttattt ggggtcaacg tggacaatcg gcgggagctg
    60001 gtggtcacac aggtatgttt gttgatgctg ataacattat acattgcaat tacgcaagca
    60061 ataacatcac gattgataac tacaatcaga cggctgctgc tagcggttgg atgtactctt
    60121 atgcttatcg ttatgtcgga gctaccgaca catttgtcgg tagcaaaaga attgatgagc
    60181 tagctcagga ggttctagct ggcaaacatg gtagcggtga gcagcgcaag ctatctcttg
    60241 gtagtaatta tgatgccgtg caaacaaaag taaacgagat gctcaaacaa ccacaagtag
    60301 cagagcaaag cccagcagtc aaacaagacg gagacttatt gtttaatggt gtggtgctta
    60361 aaagggctat tttggataaa atcctagcca aatgtaaaga gcatgacatc ttgccaagct
    60421 atgctattac tgtgttgcat tttgaggggc tttgggggca gtcagctgtg ggacgctcag
    60481 acaacaactg gggcggtatg acgtggccag gtaaaggaga gcgtccaagc ggtattactg
    60541 ttactcaagg tacatcccgt ccgttaaatg agggtggtca ttatatgcat tacgaaagtg
    60601 ttgacgactt tttgacagac tggttttatc tgttacgagc tggaggcagc tacaaggtta
    60661 gcggcgctaa gactttcagt gaggctgtga aaggcatgtt tattatcggt ggtgccaaat
    60721 atgattacgc tgctagcgga tttgatagct atattgcagg catggcaagc cgtctaaaag
    60781 cgattgagca ggaaaatgga ccacttagca agtataatca acagaccgac atcaatgtcg
    60841 ggcagtctga caagattgat gtggttattg atagcctgga gattactatt aatggtgtca
    60901 attacaccgc aactaaaaaa ccaatttagg aggtaaagct ccgagataag acaaacgccc
    60961 tcagcaatcg ctgggggctg ttttttcgca aaaaacttcg catttattga caaaaagttc
    61021 aaggcgtgtc ataatgaggt tggttataga agataaattt cgttcattta tcctcctttc
    61081 taacccaacg tcctcgctgt tcgttgaacc cgtagtgata tgggcgtatg actgaaagca
    61141 catcatacgg cttggcagag cttaagaact gttctcttgc gataagccta agaagcacaa
    61201 tagagagtta gagttttgcg tctctaatcg ttagccctga ccggaggata tttccggtcc
    61261 gtgctttttt attttgtaga aagttttttg atagtggaac tcaaagaaat agttaatgat
    61321 tatgagctaa atttttgtgg gaaaaggtgt aaagttgaga ctaattttaa gcatttaccc
    61381 gaatttatga ttttatttga tataagagat ttgtaccatc ttctaggcat tcataagttg
    61441 aaaacaaagt atcgcgcaac aaattgggtt gaagctgtga aagcagatgt tttcctctta
    61501 tcgaattatt caaagcatcc aaattttaga gaagttcttc ctagagttgg taattataat
    61561 tttttatatg agatattcta tcagtttaga gttaacatct gtattttaga taaggattta
    61621 actaaaaata caatgaaact gagtgttgtt ttttataaag acaacaagaa gaaattagta
    61681 gttgtaggat taaaaagaga tgagacaggg gtcttcaggc cagctacgtt gcatgagagc
    61741 cgaaacaatc cctacaagcg aattcggcat actgctataa aatcaataac ttggatttag
    61801 cgcatacctt ttgtgctgct taccttcgtt gacaccctcg cacaatcatg aaccaattac
    61861 catagtaaga attagccgaa ataataaatc aaccgctcag ataaatttct gggcggttgt
    61921 tttttatagt gcagtcaatt tatgttaagc ggcttcttca aactcttcgg tttctacgtt
    61981 actatcttct tcgttttgag ttgaatctag catcattcct gtttggtaat ccaattcagc
    62041 taaaggagat acgttgtcta gtgtaactga tgttacgccg tagttatcta cactttcttt
    62101 ttcaccacct aacttaattt gaagtagatt tccatcttca tcaattccaa catattgtag
    62161 aattacttgg cgagggacta actcattttc atgataaatg ggagtaactt tatagtctag
    62221 ccagaagtta ggatgtaaag ctaaccaact gtctaatctg ttttcgtaat agagcattcc
    62281 taaaggattt ctgtcactaa aaccagtatt aaggtatttt gtcattgtaa ctagattttt
    62341 aggttcgtca tttaaaccac taaattgata gccaaccaga tggccgcgat ccattaacca
    62401 agttgttttt ccattagcat cagtcagctt gtagttatgc cagccaggag gattgaattt
    62461 aagtcctttt cgctgaattt taggctcatc ctgatcttta agctgtatgt gtgcgaaggt
    62521 tggtcttcgc agattatcaa ggtcacccaa aactaatttg tagctaccag taaatggcaa
    62581 gattccaggt gcttcagtcg ccttcgttcc tgacaaaatc tccgtggtag tagggaaatt
    62641 tctaattttt ttggctgccg tggcagtact tgttgtgaaa gtggttagta tagctattaa
    62701 aatgacagct aaaccttgcc aaatacgacg atttgcttta gacatatcca tgtcctcctt
    62761 taattattta caggttaagt ataccacttt aaattaaaaa aacttaaaat taataaaatt
    62821 aatttgttgc caatattgta ctaaaatttc tttttattta atattcgcta ataataaata
    62881 ttttagtttg taacaaaaat agatgttatt taattttaaa taatctttaa atttaattat
    62941 gttatttcct gtaaaatacg aataataaga taaggaggct ttttatgcta acatacgatg
    63001 agtttaaaca ggcaattgat gacggatata tcacaggaga caaagtcacg attgtgcgca
    63061 aaaacggaca gatttttgat tatgtgttgc cgcatgagga gataagagat tgggaggttg
    63121 tgacagacga aaaagtggaa gaggtggtgg tggaattaga caaataaaaa agacttttcc
    63181 aagtccaaaa atcagatgct ccccagcgca aaaattacta cgttatttac tacgttgttt
    63241 ttaaaatcag tgtaattgac cagataatgt aaggttgtaa aatgttgatt tatcaagtga
    63301 ttgggcaatg ataccgatta agtttaaggt gtctgggatt tatgttataa tagtcctatg
    63361 gctagaattt tagacaatga tgtaatggga aatgaggagt tttcggatcg gactcttcgc
    63421 cctcaatatc tacacgaata tattggacaa gataaagtta aggaacaatt cgctattttt
    63481 atagaagcag ctaaaagacg tgacgaatct ttagaccatg ttttattatt tggacctcca
    63541 ggactgggaa aaacaacaat ggcatttgtg attgccaatg aattgggagt taatttaaag
    63601 caaacttcag gtcctgccgt tgagaaagct ggagatttgg tagctatttt aaatgaatta
    63661 gaaccagggg atattttgtt tattgatgaa attcaccgca tgccaatgtc tgttgaggaa
    63721 gtactatata gtgctatgga agacttctat attgatatta tgattggcgc tggagatact
    63781 agtcgtagta ttcatttgga tctgcctcca tttacattga ttggtgctac tacacgtgca
    63841 ggcatgttat ctaatcccct cagagctcgt tttggtatca ctggtcacat ggaatactat
    63901 caagaaaagg atttgacaga gattgtggag cgtacagcta ctatttttga aataaaaata
    63961 gaccatgaag ctgcacggaa attagcttgt cgcagtagag gaacgccccg cattgctaat
    64021 cgattactaa aacgagtgcg tgactatgct caaattattg gtgatggcat cataacggct
    64081 caaataactg atagagcttt gactatgtta gatgttgacc gcgaaggtct cgattacatt
    64141 gatcagaaaa ttttacgaac gatgattgag atgtatcagg gaggaccagt tggtttgggg
    64201 acattatctg tgaatattgc cgaggagcgt aatacagttg aagagatgta tgaaccctac
    64261 ttaattcaaa aaggctttct catgcgaaca cgaacaggtc gtgtagcgac tcaaaaggct
    64321 tatcgtcatc ttggctatcc ctatcaaaac acttaatgat ttttcaaatc taagaaaatt
    64381 aatctgaacc tgctttttgt gttcggattt tttgataagt tgacagaaaa ttattagatt
    64441 gatttctccg atatcagtta gaaatttgat ataataattc aaaggagtat ttaatgaaaa
    64501 aagtatgttt tgtctgtctg ggtaatattt gtcgaagccc catggctgag tttgtaatga
    64561 agtcaatagt atcaagtgat gttatgatga tagaaagtcg tgctacttct gattgggagc
    64621 atggaaaccc tattcactca ggtacacagt ccatattaaa aacttaccaa attaattatg
    64681 atataactaa atgctccaag cagataacta taactgattt taacaccttt gactatatta
    64741 ttggtatgga tagcgataat gtgaaaaatc tcaaagagat gtcgcaacat caatgggact
    64801 ctaaaatcta cctgtttaga gaagggggtg ttcctgatcc ttggtacact aacgattttg
    64861 aagaaaccta ccaattagtt agaaaaggat gccaggattg gctttctcgt ttaatgagta
    64921 aagagtattg atattagaag ttgagtaaaa gaaatgatag atatgcaaga tatagtaaaa
    64981 aaatggagta ttacacgggc gaagttggaa attgtctcgg tgattgtcat attggtttgt
    65041 gcaatttctg tttttagcgt aagaatttcg aataagacaa gtttgaccta tgataaaggg
    65101 cgtatgcact atactggtta cgtgatcaac cataaaatga atggagaagg gaagcttgtc
    65161 taccccaacg gtgatatcta tgagggtact tttaagaatg gcctatttga aggaaaagga
    65221 acttttactg ctaagacggg ctggttatat aacggggagt ttcataaagg acaagcaaat
    65281 ggcaaaggtg tgttaaaagc aaagaataat aaagtctata aagggatttt taaacagggg
    65341 atatttcaaa aatgagaata aaatggtttt cgtttgttag ggtaacagga cttttactgg
    65401 tttt
//
LOCUS       DAUVUY010000003        48237 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.003, whole genome shotgun sequence.
ACCESSION   DAUVUY010000003 DAUVUY010000000
VERSION     DAUVUY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48237
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.003"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..1538)
                     /locus_tag="VOX58_000168"
     CDS             complement(<1..1538)
                     /locus_tag="VOX58_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HES5877447.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNY"
     gene            complement(1742..2500)
                     /locus_tag="VOX58_000169"
     CDS             complement(1742..2500)
                     /locus_tag="VOX58_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5877448.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            complement(2497..3366)
                     /locus_tag="VOX58_000170"
     CDS             complement(2497..3366)
                     /locus_tag="VOX58_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5877449.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERYFKGVRLTR"
     gene            complement(3711..4709)
                     /locus_tag="VOX58_000171"
     CDS             complement(3711..4709)
                     /locus_tag="VOX58_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HES5877450.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANRQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKNLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVIDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
     gene            5063..6715
                     /gene="fbp54"
                     /locus_tag="VOX58_000172"
     CDS             5063..6715
                     /gene="fbp54"
                     /locus_tag="VOX58_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HES5877451.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRVQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSEMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            complement(6774..8021)
                     /locus_tag="VOX58_000173"
     CDS             complement(6774..8021)
                     /locus_tag="VOX58_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HES5877452.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDMEGLTEVAREKLLQAVSISPEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLLYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(8011..9192)
                     /locus_tag="VOX58_000174"
     CDS             complement(8011..9192)
                     /locus_tag="VOX58_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HES5877453.1"
                     /translation="MPLIPDKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            complement(9250..9726)
                     /locus_tag="VOX58_000175"
     CDS             complement(9250..9726)
                     /locus_tag="VOX58_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HES5877454.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            complement(9947..10987)
                     /gene="rfbB"
                     /locus_tag="VOX58_000176"
     CDS             complement(9947..10987)
                     /gene="rfbB"
                     /locus_tag="VOX58_000176"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HES5877455.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(11231..11824)
                     /locus_tag="VOX58_000177"
     CDS             complement(11231..11824)
                     /locus_tag="VOX58_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HES5877456.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            complement(11824..12693)
                     /gene="rfbA"
                     /locus_tag="VOX58_000178"
     CDS             complement(11824..12693)
                     /gene="rfbA"
                     /locus_tag="VOX58_000178"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HES5877457.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHELAQSLKKN
                     EYGQYLLRLIGEA"
     gene            complement(12752..13858)
                     /locus_tag="VOX58_000179"
     CDS             complement(12752..13858)
                     /locus_tag="VOX58_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HES5877458.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGDDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KTSGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLMPLGYQVDVRPQKGQ
                     LLDYDVHHIISDNYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKSCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSTGKA"
     gene            complement(13898..14686)
                     /locus_tag="VOX58_000180"
     CDS             complement(13898..14686)
                     /locus_tag="VOX58_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HES5877459.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMERDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            complement(14676..15362)
                     /locus_tag="VOX58_000181"
     CDS             complement(14676..15362)
                     /locus_tag="VOX58_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HES5877460.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIT
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            complement(15434..16090)
                     /gene="nth"
                     /locus_tag="VOX58_000182"
     CDS             complement(15434..16090)
                     /gene="nth"
                     /locus_tag="VOX58_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HES5877461.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            complement(16087..16770)
                     /locus_tag="VOX58_000183"
     CDS             complement(16087..16770)
                     /locus_tag="VOX58_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HES5877462.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            complement(16851..17369)
                     /locus_tag="VOX58_000184"
     CDS             complement(16851..17369)
                     /locus_tag="VOX58_000184"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HES5877463.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            complement(17519..19729)
                     /gene="recJ"
                     /locus_tag="VOX58_000185"
     CDS             complement(17519..19729)
                     /gene="recJ"
                     /locus_tag="VOX58_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HES5877464.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYERGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            complement(19726..20490)
                     /locus_tag="VOX58_000186"
     CDS             complement(19726..20490)
                     /locus_tag="VOX58_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HES5877465.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAQEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGTFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(20490..21419)
                     /locus_tag="VOX58_000187"
     CDS             complement(20490..21419)
                     /locus_tag="VOX58_000187"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HES5877466.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFVVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(21434..22069)
                     /locus_tag="VOX58_000188"
     CDS             complement(21434..22069)
                     /locus_tag="VOX58_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HES5877467.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(22062..23300)
                     /gene="hflX"
                     /locus_tag="VOX58_000189"
     CDS             complement(22062..23300)
                     /gene="hflX"
                     /locus_tag="VOX58_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HES5877468.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(23373..24272)
                     /gene="miaA"
                     /locus_tag="VOX58_000190"
     CDS             complement(23373..24272)
                     /gene="miaA"
                     /locus_tag="VOX58_000190"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HES5877469.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLTDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            24400..24576
                     /locus_tag="VOX58_000191"
     CDS             24400..24576
                     /locus_tag="VOX58_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HES5877470.1"
                     /translation="MIMEYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(24694..25602)
                     /locus_tag="VOX58_000192"
     CDS             complement(24694..25602)
                     /locus_tag="VOX58_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HES5877471.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFSYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
     gene            complement(25661..26047)
                     /locus_tag="VOX58_000193"
     CDS             complement(25661..26047)
                     /locus_tag="VOX58_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HES5877472.1"
                     /translation="MLFIAKQTLKSQLIPQDNLLAESRFNEIMDFLTGDFPLVFRPMY
                     NPHRYTISQDNQALEKVKQASYKRMDIAMTHLDGLIGDSGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLAAFMAKMLEM"
     gene            complement(26358..27344)
                     /locus_tag="VOX58_000194"
     CDS             complement(26358..27344)
                     /locus_tag="VOX58_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HES5877473.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQGWAERLEAIGESLPEFVGA"
     gene            27544..27846
                     /locus_tag="VOX58_000195"
     CDS             27544..27846
                     /locus_tag="VOX58_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877474.1"
                     /translation="MTYELCLEYGTYPLSRVYAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
     gene            complement(28209..28280)
                     /locus_tag="VOX58_000196"
     tRNA            complement(28209..28280)
                     /locus_tag="VOX58_000196"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28245..28247),aa:Arg,seq:ccg)
     gene            complement(28393..29598)
                     /gene="rpsA"
                     /locus_tag="VOX58_000197"
     CDS             complement(28393..29598)
                     /gene="rpsA"
                     /locus_tag="VOX58_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HES5877475.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            complement(29725..29796)
                     /locus_tag="VOX58_000198"
     tRNA            complement(29725..29796)
                     /locus_tag="VOX58_000198"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29762..29764),aa:Gln,seq:ttg)
     gene            complement(29847..29927)
                     /locus_tag="VOX58_000199"
     tRNA            complement(29847..29927)
                     /locus_tag="VOX58_000199"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29891..29893),aa:Tyr,seq:gta)
     gene            complement(29988..30218)
                     /locus_tag="VOX58_000200"
     CDS             complement(29988..30218)
                     /locus_tag="VOX58_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HES5877476.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            complement(30282..31349)
                     /locus_tag="VOX58_000201"
     CDS             complement(30282..31349)
                     /locus_tag="VOX58_000201"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HES5877477.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            complement(31427..33886)
                     /gene="parC"
                     /locus_tag="VOX58_000202"
     CDS             complement(31427..33886)
                     /gene="parC"
                     /locus_tag="VOX58_000202"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HES5877478.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKDADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKTDAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            complement(33977..35926)
                     /gene="parE"
                     /locus_tag="VOX58_000203"
     CDS             complement(33977..35926)
                     /gene="parE"
                     /locus_tag="VOX58_000203"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HES5877479.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGSVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVESIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKTEKVSYAWTDGEL
                     EDLRKEFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            36061..36702
                     /gene="plsY"
                     /locus_tag="VOX58_000204"
     CDS             36061..36702
                     /gene="plsY"
                     /locus_tag="VOX58_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HES5877480.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            complement(36760..38028)
                     /locus_tag="VOX58_000205"
     CDS             complement(36760..38028)
                     /locus_tag="VOX58_000205"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HES5877481.1"
                     /translation="MLLIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAASGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLIVGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(38152..38805)
                     /locus_tag="VOX58_000206"
     CDS             complement(38152..38805)
                     /locus_tag="VOX58_000206"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HES5877482.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGIDWLQ"
     gene            complement(38938..39588)
                     /locus_tag="VOX58_000207"
     CDS             complement(38938..39588)
                     /locus_tag="VOX58_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HES5877483.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFLTGLVFGLLLALLGR
                     SHQPLLHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            complement(39617..40480)
                     /locus_tag="VOX58_000208"
     CDS             complement(39617..40480)
                     /locus_tag="VOX58_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HES5877484.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWETIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DAQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPIIDTKDVN"
     gene            complement(40598..42052)
                     /locus_tag="VOX58_000209"
     CDS             complement(40598..42052)
                     /locus_tag="VOX58_000209"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HES5877485.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGKLFAGVPIFLKDLGQELKGDLSTSGSKLFKNHHATKTDLFVK
                     RLEDLGFIILGRSNTPEFGFKNISDSQLHGPVSLPHDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKNSIYQSLQRPLTIAFYQRLSDGSPVSLDTEKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            complement(42249..42878)
                     /locus_tag="VOX58_000210"
     CDS             complement(42249..42878)
                     /locus_tag="VOX58_000210"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HES5877486.1"
                     /translation="MTLGSQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            complement(42936..43628)
                     /gene="pyrF"
                     /locus_tag="VOX58_000211"
     CDS             complement(42936..43628)
                     /gene="pyrF"
                     /locus_tag="VOX58_000211"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HES5877487.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRSIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            complement(43905..44441)
                     /locus_tag="VOX58_000212"
     CDS             complement(43905..44441)
                     /locus_tag="VOX58_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877488.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     EAFIKYYQSPKVPTGEFE"
     gene            44663..45568
                     /locus_tag="VOX58_000213"
     CDS             44663..45568
                     /locus_tag="VOX58_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HES5877489.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLNMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHSKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            complement(45647..46435)
                     /locus_tag="VOX58_000214"
     CDS             complement(45647..46435)
                     /locus_tag="VOX58_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HES5877490.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRIIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKTELGLWRQSIV"
     gene            complement(46428..47141)
                     /gene="deoD"
                     /locus_tag="VOX58_000215"
     CDS             complement(46428..47141)
                     /gene="deoD"
                     /locus_tag="VOX58_000215"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HES5877491.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGRRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVALETLIAND"
     gene            complement(47726..>48237)
                     /locus_tag="VOX58_000216"
     CDS             complement(47726..>48237)
                     /locus_tag="VOX58_000216"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HES5877492.1"
                     /translation="KALGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENL
                     DEFGPRFPDMSDAYTKVYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVL
                     GADAVGMSTVPEVIVAAHSGLKVLGISAITNFAAGFQSELNHEEVVEVTEHIKEDFKG
                     LVKAILAEL"
BASE COUNT        14518 a        10312 c         8530 g        14877 t
ORIGIN      
        1 aagtaattgc caacagttgt attaactaat tctgcacttt ctcttaaaat atcatgactt
       61 ttcttgacat acacaaatcc acgcgtatta actttagcct tagaaataat gcgtttttct
      121 tttttagaaa cagtaatagc aacaatgaaa ataccatctt ctgacaagac tttacggtct
      181 cgtaaaacga tattacccac atcaccgata gcattaccgt caatcatgac atcactggca
      241 ggtacacctc cttcatggag aaaaccttcg tcattaagca ccatgatatc gccacgcttc
      301 agaatatgaa tattctctgg aaaaatacca acttcttcag ctagctttgc atgggcagct
      361 aaatcacgat attctccttg cactggaaaa agatactggg gttttaacaa attcatcaat
      421 agttgcaaat cacgcccatt agcatggcct gacacgcgta agttttgagt gattaattta
      481 acagaaccac cggccttgta aatcaagttt tctacacgcg ccaccatagc ttctttagcc
      541 gtacttggtg tagttacaat ataaacaagg tctccttctt tgatttgaac ataacggtga
      601 cgtcccgctg ccattttttg taagctatta attggctcac ccatacgacc agcttcaagg
      661 atgattaatt catggtcttc gaatttagac atatccttag gtttaattaa aagacgctca
      721 tccgtaatca ttaatttttc tagacgtaaa gctgttctga caatattttc tgcatctgtt
      781 ccggttaaaa caacacgacg cccatgggca gtggcggaat caaatacttg ctgaatccga
      841 acaagatttg aggctactgc agctacaatg acacggccat cagcatcaga gataacactg
      901 tccatttctt caccaacttc agattcgctg gcaatctgat cgttgcttgt cgcattgact
      961 gaatctgata aaagagctag aaccccttct ttcccaatct cagctaaacg tagcaaatcc
     1021 gtctgataac cttctcttgc tgcttgatca aatttgaagt caccggtata aacaatgttt
     1081 cctttatctg ttccaataac aatacccata ctttctggga ttgaatgcgt tgttctaaag
     1141 aacgaaacta ggccatcttt aaattcaatt tcagtatcac tgtctaccac atgaaaatta
     1201 ttaaatttct tagtgctgtt attacttttc acaaatagct tggctaattc aatagtcaat
     1261 tcagatccaa atacaggagc tgatacttca gctaacaaat aaggaagggc tccaatggcg
     1321 tcagcatggc catgactcaa gaaaatccct tgtactttac ctttgttttc aattacataa
     1381 tctaaattgg ggatgactaa atctacccca agttgctcat tttcagggta cttgagccct
     1441 gcgtctaaaa taaacataga atcgttaatt tctaccaaat agaagttttt tccgtattca
     1501 cggacccctc ccaaggcaat cattttaata tcagtcatga tactccttta aatattttat
     1561 tattacaaac ggtccttaca acgtaagtcg aattactgtt atgtagctat gctactatct
     1621 tctatagtat atcatatttg aggcgttttt tctctaaata gccttgatac tttcagatta
     1681 ttttcagtat ggaaatcaaa aaatcactga cttattatta gtcaatgatt tagatcgttg
     1741 attagtcaaa tagttggtaa tagtcagtaa tcgtcagttg ttctttttcc atttgattta
     1801 aatctttaat aatgtttcca tctttcatta caatcagacg attcccataa gttaatgcat
     1861 cttccatatg gtgggtaatc atcaaagctg ttaagccatc ttttgtcaca aactcatctg
     1921 tcaattgcat taaagattga ctcgtcttag ggtcaagggc agccgtatgt tcatctaata
     1981 ataagagagc tggctttttc aacgttgcca ttaataaact taaagcctgt ctttgtccac
     2041 cagacaaaag acctgctggt gtttctaaat gcttttctag gccatttcct gttcgtttaa
     2101 ccaaatcctc aaaactagct aagtgctctg ttatttttcg agaagccaag gagcgttttt
     2161 ctcctctttg acgtgcgatt aagagatttt cagccactgt catccgaggg gctgttccca
     2221 ttttgggatc ttgaaagact cgcgataggt aaagagctct tttttctgcc ggccaatgcg
     2281 tcacatcctg accaagaatc ctaatttgcc cccgtgtcaa acttagcgtt cctgcaatta
     2341 cattaaaaag ggttgatttt ccagctccat ttcctcctaa aatagttaaa aaatcatgtt
     2401 catagatagt tagtgtcaca ttgtccagta ttgttttagc atcttcaaaa ccattatcta
     2461 cgtcaactgt tgcattgatc aattcaataa tttttgtcat cgtgttaacc tcactccctt
     2521 aaagtagcgc tcttttacca ctggtatcat gaggcacagt gctaaaacaa gagcactaat
     2581 taatttcaaa taacttgtat taaaacctag cgtaataact actgaaatta aaaattgata
     2641 aagaatcgag cctattacaa tagcaatcaa acgttctaaa agagtcagtc cagttgagta
     2701 tagtacttca ccaacaatta tgctcgccaa accaataaca ataactccaa tcccttttga
     2761 gacatctgca tagccatctt gttggctcac tagagcacct gataaagcta ttaaactatt
     2821 agaaaccaca agtcccataa cttccatcca atccgtgtta ataccaaaac tcttagccat
     2881 atctttattg tctcccgtag cgatataagc ttgacctaga ttagtgtaaa ggaaatagat
     2941 caaaacgcta ataacaatca caacagttat cagacctgtt aaaaggctat ttaaatcaat
     3001 tgaaaaaggt aaacaatcct gaatacgttt atgatcatgt aaacccaagt ttgctcttcc
     3061 cattaccatt agcataatgg agttacatga cgtcatcacc aaaattccag ctaaaagtgt
     3121 tggcattttt cctttcgtat ataacagccc tgttaaaaaa cctgctaaag cacctgataa
     3181 cataccaagt aaggttgata gaaatggatt ccaccctaag ctaatagcgg tgaccgcaac
     3241 agctcctcct aaaggaaaag aaccttctgt agtcatgtca gggaagttta aaatcctaaa
     3301 ggtcaggtag attcctaatc ctaaaactcc ccatataagg ccttgtgaaa ctgatgaaat
     3361 aatcatattt ttcctcatat ttatattagc gggtaactct cgccagtata aaccctttct
     3421 ttacattaat ttttagattt gttttatact tagttcagca ccgtggctta gtcttttttc
     3481 caatcacttt aacgaaacta agacagaaaa caaaaaaatc gagaatcatg tttaaactta
     3541 ctcaaaaggc aagatttttt acgtgtctga aatagagcat tttaaaacta aaaatgataa
     3601 aaataaccct tacatcctca agacgattgt ctgacttggc atccaaattc atctcagtga
     3661 gggtgttttt tatggttttt tcttgacctt ttatatcaca tgtcattgta ttaattctca
     3721 ataaaagaag ctgaaggaaa atcttccctg ttaatagcaa tacctaattc tgctgctaca
     3781 ttcttattaa ttagcggttg accaatatcc acaactttga caggaacatc tataacacgc
     3841 tttcctttga ttaattttaa cacctgatta gctgtttgaa ctcccaaatc gtactgattt
     3901 tgagaaatgg ctgccaaacc acctttttca accattcgat caacgctagt gtagacagga
     3961 atctttgcag ccttactggt tgtcatgact gatgaaaatg cacttgctat agtattgtct
     4021 tgggggataa ataccgcgtc tactttacct aacataactg acatcgtggc aggaacttcg
     4081 ttagtagaag gaaccgcgta tgaaataact tgatagccct ttttgcgagc taaccgccta
     4141 aactctttaa cttgagataa ggaattatcc tcattacttg catataaaat tccaactgtt
     4201 ttgacatgtg gtgtcagatt tttcattaaa tcaattgtct gtttaacagg cactttatta
     4261 gatagaccag ttacattagc ttcaggttga tccaattgca tcactaatct agatcctact
     4321 ggatcagata ctgctgacat gacaactggg atatccttgc tggcagctgc taagccttgc
     4381 gccgccgggg ttgcgatacc aatcacgata tcacttcctg attgcactaa ttgcctactc
     4441 attgtttgga ttttactctg atccccctca gcattcataa ccttaatcac tacattttgt
     4501 ttttgaggca tctgcttttt gagttgatct tcaatacctc gcttaatatc atcaagagcc
     4561 tcatgagtaa cgaattgtaa gataccaatt gtaatgtttt gtcggttagc caaatcacgg
     4621 ttttcctttg atactccttt tgataataga gagcctatca ctattacagt taaaacaagg
     4681 agggtagcta ttaaactctt gtttttcatc tgaattcctc acatttcatg tcaatctctt
     4741 taaaacgttg atgatgagaa tacttctcaa aagaaaaagc acttagtaat gattactagg
     4801 cgcccttgtg aaatccaatg tcagcgcata gatatctttt tagaagtact ctaaaagtcg
     4861 tatctatgcc aattcataaa cacaacttat ctatgccagc cttgccaatt tactaagtgt
     4921 tgtgttttta ccatcattga cttcctttct cgttttttta acattataac ctaaatcaaa
     4981 aaaattgcaa gctctttcgt ccatattttt agtcaagtat ttgtggtata atgaagagat
     5041 tagaattcag aaaggagaaa ccatgtcttt tgacggcttt ttcttacacc atttaaccaa
     5101 cgaattaaaa gagaacttgc tttacggtcg tatccaaaag gtcaatcaac cctttgaacg
     5161 ggaacttgtc ttaaccattc gtaaccatcg caagaattat aaattgctat tgtctgccca
     5221 tcctgttttt ggaagggtac aaatcaccca agcagacttt caaaatcctc aggtacctaa
     5281 tacctttacc atgattatgc gtaaatacct ccagggtgca gtcattgagc agttagaaca
     5341 gattgataat gaccgcatta tcgaaatcaa ggtatctaat aaaaatgaaa tcggagatgc
     5401 tattcaggca accctaatca ttgaaatcat ggggaagcac agcaatatca tcttggtgga
     5461 ccgtgcagaa aataaaatca tcgaatccat taaacatgtt ggcttttcac aaaactctta
     5521 ccgcaccatt ttacctggct ctacctacat tgagccccct aaaacagctg ctgttaatcc
     5581 ttttacaatt acagatgttc ctctatttga aatcctccaa acacaagaac taacagttaa
     5641 aagcttgcaa cagcattttc aaggtttggg gcgagacacc gcaaaagagt tggccgagct
     5701 tctgaccaca gacaaattaa aacggttccg tgagtttttt gctagaccta ctcaggcaaa
     5761 tctgaccaca gcttcttttg cgcctgttct tttctcagat agccatgcga cgtttgagac
     5821 tttatcagag atgcttgatc acttttatca agataaggct gagcgtgacc gtatcaatca
     5881 gcaagccagt gatttgattc accgtgtgca aactgaactg gacaaaaatc gtaacaagct
     5941 aagtaagcaa gaagctgagc tgctagccac tgaaaatgct gaattattcc gtcaaaaagg
     6001 agaattattg accacctacc tcagtctggt cccaaacaac caagactctg tgattttaga
     6061 caattattat acaggggaaa aaattgagat tgccttagac aaggctctga caccaaatca
     6121 aaatgctcaa cgttatttta aaaaatacca aaagctaaaa gaagctgtca aacacttatc
     6181 tggattgatt gccgatacca agcaaagtat tacttacttt gagagcgtgg actacaattt
     6241 atctcaagca agtatcgacg acatcgaaga tattcgcgaa gaattatatc aggctggctt
     6301 tttgaaaagt cgccaaagag acaaacgcca taaacgtaaa aagcctgagc agtacctggc
     6361 ttcagatgga aaaaccattc tcatggtggg acgcaataac ttgcaaaatg aagaactcac
     6421 ctttaaaatg gctaaaaaag gagaactctg gttccatgct aaggatatcc ctggaagtca
     6481 tgtcatcatc aaagacaatc ttgacccaag tgacgaggtt aaaactgatg ctgccgaact
     6541 agctgcttat tattcgaaag ctaggttatc aaatctcgtt caggtcgata tgattgaagc
     6601 taaaaaatta cacaaaccta gtggcgccaa gccaggattt gtgacttata ctggtcaaaa
     6661 aactttacgg gttactcctg accaagccaa aattctctct atgaaattat cctgataagc
     6721 aactaaaaaa cagctctcct aggaaagtta tttcccctag gagaactgtt tttttattct
     6781 gattgttggt gcttgatatg gtcaagaaag tcttgcatat tcacatcatc aggcacttgt
     6841 ctgaggtaag cggtagccat ttggatagcc ttgtgaaact ggccaaactc ccttaataaa
     6901 taggcataat cctgtaaaaa ctccggattt tctgccaaat cagccgaaat ctcattataa
     6961 agagccaagg caacttcttc ttgttccaag gcgtggtaag cctttgcaat ggtccatttg
     7021 gttaagacat tatcaatcgt ctcacgatta agagcaataa cctcctcaaa acgctcggca
     7081 tcaaaataaa gggtcactaa acgcatgaga atctcttcgt catcaacagc tacttccttt
     7141 gcttgaagca ggtaattttc agcattctgc ctatcgtgta attcataaga aagctgcgat
     7201 gctagtaata ataattgact gtcaaatgca tttttacgta ggccctgttg aaccaagcgc
     7261 aaagcttctg acgtcttatg ctcttcatga agactcaaag cataaccata ttcataacct
     7321 ggataatctg gattgatggt ttccaactgt ttaaagtaaa gattagcttt ttgataattt
     7381 tcttggtcat acaagagggt cgccaattca aaaacagttt catcttcgta ttcaatggcc
     7441 acggcctttt ccaagaattc gatggctgcc tcaaatttcc ctaagctagc ataggctcgt
     7501 ccaatgcgtt gataagtgga aatacctgtc aattcaagga tttgacgatt gtctaactgt
     7561 gcatagtaat caatagcttc tttaaaatac tgcaaggaca tgtcaatttc tgccaaacca
     7621 aaaatgacca atggctctgg gctgatgctc actgcttgaa gcaatttttc acgcgccacc
     7681 tctgtcagtc cttccatgtc atagaggtca gccattacta agagagctga caaataattg
     7741 gggctatctt ttgaaacctt atccaaatac aagaaagctt cttcaatggc gtcgtcttct
     7801 gctgcaatct gagctaaatt tatattgagt tctgggtaat catcagctaa ctgcaaataa
     7861 atacgcttgg catgtggcaa aaaacctata ctttctaagt attctcctaa ggcgattaaa
     7921 ctatctgcat catcttcttt taaagctttt tggaaatatt tttcagcgtg cgctaggtct
     7981 tgctgatcca aggaagcaat cattttttca ctatttaaca tggtccttaa cctcctcaat
     8041 caccaatact tcttcgtcat acaaaccgct aacttttttg taccagtcaa ataattcttt
     8101 aatcacaact ttgacagaag cgtagattgg gatgactaag aagacacccc aaacgccaaa
     8161 cattgatcct gcagtcaaca ataagaacat gatcgtaatc ggatgaatgc tcagcttatt
     8221 tccaagaaca agcggtgcca caaaacggcc ttcgatggtt tgctcaatca taaagataac
     8281 aagcacctta accaacataa atgggccttg taccatagcc ataatcacaa caggtatcat
     8341 ggcaaggaaa cttcctaagt aaggtatcat gttaagaaag ccagcaataa tcccaaaagt
     8401 gatggcatat ttcagtccca caagactaaa catgatagaa aacataaagc cgacaacaat
     8461 tgcaaccgta acctgccctt ggacatagcc tgataattgc ttgttaatat ctcccaagac
     8521 tcgaaccata ggcacccgta atttgagagg taagacattc acaaggccat ttttcatgtg
     8581 gctactgtct cgtaaaaaat aaaagagaat aaagggcgaa ataataatag ccacggtaat
     8641 ccgagcaatg gcaccagcaa aattaccagc ccagtcaata gcattttttg aaaaactttg
     8701 cgcgtaatcc aaagcctttt gacttgtatg ggtaagcatg tcttggagtt ggggcttgta
     8761 gctgactaac cattcatttt caagaagttt attggcttct tcattgacct tgcccacata
     8821 tttgggcaga tcctcaataa aactggtaag ttgggtctgt accatgggaa caaagcctga
     8881 aatcgcccag acaaaaagta aggtaatcaa accaaaaaca ataaaaatag aggtggtacg
     8941 attcgggccc aagtgattaa taaggtcaac taagggtttg gtcagataat acagaatggt
     9001 cgaaataacc aagggcaaca tgatgaccgc aaaaaaagaa atcactggca taaataaaaa
     9061 agagatttta gtaaaaacaa aaatcgttaa aaaggctaaa agtgttatta ctaaagccat
     9121 ggtagcttga ttatttaaaa accatttgta aaataagcta tctgttctag acttgtcagg
     9181 tattaagggc attaagatag ggttcctttc tttgggaaaa tgataagttt tactggtaac
     9241 ctgggtaaat caataaaagg tcacagattt atccatgagt tggttgtttt ggtcataggt
     9301 aaatttggca gagaaaaaag gacggtcttc taaaatccat ttaaaaatgt cataatcacc
     9361 ttcccaagtt ggcttttcca acacttgatc gtaaggtacc cattccaagg ttccttctcg
     9421 tgactcttca tcggaaatga ggtctccttc aaatcctgtt actttaaaaa cataggtata
     9481 ccagtcatga ccaggggtaa attctggaaa agtaatgatt cctttaaagg ccatctctgt
     9541 aacagtcaaa tgggtttcct ctaaaatctc acgacgggca cactcatctg gggtctcacc
     9601 agcctcgagc ttacccccaa ctgaaatcca cttgcctttg tggacatcat tttctttttt
     9661 attgcggtgt aataatagca aagaatcgcc attatcaatg taacaaatgg ttgctaattg
     9721 agtcatctat ctctttcctt tccatctttg tttctattat accaatctct agaaaatatg
     9781 ctatcatttt atgagtcaaa gtgtgaatac ttagtcacgc tcaaaacctt ctaaagatgc
     9841 cttaaaaaag tgtagataag agcctacaat gaaaaacatc aatttaaact atcaaaagaa
     9901 ccttgtcata tcaacgataa ttgatatgac aaggttcttt aatgttttat ttaatcactt
     9961 cttgagtttt agcatacttg gcttctacag catctttttc tgctttccac catgtctcat
    10021 tttctgtata ccacttaata gtttcttcca aaccttctga aaagtttgta aattgtggtt
    10081 cccagccgag ttcttcacgc aattttgtag aatcaatagc gtaacgaaga tcgtgaccag
    10141 cacggtcagt tacgtgatca taagcgtctt ttggttgacc cattttctca aggataagct
    10201 caagaacttc cttgttgttt ttctcgccgt cggcaccaat aaggtatgtt tcaccgatac
    10261 gacccttagt caaaatagcc catactcctg tagaatgatc attagtatga atccagtcac
    10321 ggacattttt cccttcaccg tatagttttg gcttgatgcc tgataaaata ttggtgattt
    10381 ggcgaggaat aaatttctca atgtgctggt aaggtccgta gttattggaa caatttgaaa
    10441 tggtcgcttt cacaccgaag gaacgtaccc atgccttaac gataaggtca gaagctgcct
    10501 tagttgatga gtaaggtgat gatggattgt attttgtttc agcagtgaat ttttcacctg
    10561 gtccttcacc ctgtcctgga aggtcttcac gaagtggaag atctccataa acttcatcag
    10621 ttgacacgtg gtggaaacgg atatcgtatt tacgagctgc ttcaagcaaa gtgtaagttc
    10681 cgataaagtt tgtatggata aatggacttg gatcttccaa tgagttatcg ttgtgactct
    10741 cagccgcata gtgaacaata gcatccgttt tggcagccaa tttgtctacc aattcagcgt
    10801 cagcgatatc accaacaact aactcaacac gatcaccaag aatagcttca atgttagcac
    10861 ggttacctgc atatgtcaat ttatcaagga cagttacatg aacatctggg tggttattgt
    10921 agacatagtg cacaaagtta gatccgatga aaccagctcc accagttacg ataatatttt
    10981 tatacataag atataaaggg acctttcgtt tccgaaaaaa tctattaaaa gtaataactc
    11041 taacaaagac gttttaggcc taagaaaaca tcgctttcta agatttgagt cccatgttca
    11101 gctcaaagag agaaactgaa ctttcctttc atcattttta gttgttactt gttacctgaa
    11161 gtagagaaga ataactcccc tcatatccac ctcaaatgat taatcccaat catctgagta
    11221 tgttgtttcc ctataggtct tttggtttca gtggtttgac atcactaaga agaggatggt
    11281 ttttatctgc ttcagaaact tctgcagctg ctagattttc ccaagtaatc ccaagacttg
    11341 ggtcagcata gtttacaaaa gcatacttag gtttcaagtc aagagcccag tagtcattga
    11401 caagataact gtaagagaca gtctctgaaa gaacttgaaa gccattagca acccctctag
    11461 gaacaaacat tccttttgaa gcatcaatca ctgtctggta gacgtttcca aaagtctctc
    11521 cctcacgaag atcaacccaa gctcctaaaa ccttgccatc atcagcaact gagatgtatt
    11581 tatcccaagg ttcagcatga agtccacgca gcacatgctg acgtgaaaaa gaaacgttgt
    11641 tttgtagttt tccttcctca aaaaaacgtt ctggaaagcc aattggcaac attttttctt
    11701 tttggaaatt ttctttaaac cagccacgat tatccccacg aacaggaata tcaaactcta
    11761 gtaagcctgg gatttctttg atttctcggc aagccaatgg tttgtcaaaa aaagtttctg
    11821 tcattaagct tctccaatca aacgaagcaa gtattgcccg tattcgtttt tctttaaaga
    11881 ctgcgccaat tcatggacat cttctttact gatgtaaccc atgcgatagg caatttcttc
    11941 cagatttgcc acttgagcgt tttgcaaacg ttgaactgtt tcaatatatt gagccgcttc
    12001 aagtagactt tcatgcgttc cagtgtctag ccatgcaaaa ccacgcccca tcaattcaac
    12061 ggagaggtca ccacgttcca agtaagcctt gttgacgtct gtaatttcca actcaccacg
    12121 cgcgcttggt ttaatgtttt tagcaatttc taccacgtca ttgtcataga aataaagtcc
    12181 agtcactgca aagtgggact taggcacttc tggtttttct tcgatagaga tagcattcat
    12241 gttctcatca aattccacca ctccaaagcg ctcagggtct ttcacttgat aaccaaaaac
    12301 ggtggctcct ttttctttgg cagctgcctt ttgaagcatt ttagtgaggc cattaccgtg
    12361 gtaaatatta tctcctaaaa taagagcaac acggtcatca ccgataaact cctctccaat
    12421 aatgaaagct tgtgctaaac catctgggct tggttgttcc ttataggata agctaatgcc
    12481 aaattctgac ccatctccta gcaattcttc gaaacgagga agatcttggg gggttgaaat
    12541 gattaaaata tctttaatcc ctgccaacat caaggttgac aatgggtaat aaatcattgg
    12601 tttgtcataa atgggcatca actgtttaga tgcggcacgg gtcagtggat acaagcgtgt
    12661 ccctgaacct cctgcaagaa taataccttt catacgtagt gctcctattc ttctatatac
    12721 cctatcattc tatcattttt tgaaagcagt gtcacgcttt accagtagac cgcaaaaggt
    12781 aaggctcagg ggaatagtcg ctaggcgtta aaagtccttc gtgtcctagc aataattgag
    12841 ctaattcata gccaatcaaa gggccaactg ttaatcccga tgaccctaaa ccactagctg
    12901 tataaaggtt cttcaatcct ggaacctgac cataaaaagg agaataatca ctggtataag
    12961 ctcttatccc tacacgacat gatttttgag tagcttcttt taataacggc aaataagtga
    13021 gagcttgcat ttcaagtttt tctaagacct gccaatcagg ctccaaatca taacctttat
    13081 cattttcatg gctcgcacca acagaaatct taccttgatt gaaaggaatg aggtctattt
    13141 caccttctgg cataacaacg ggataattgt cgctgatgat gtgatgaacg tcataatcca
    13201 agagttgtcc cttttgtggt cgcacatcaa cctgatagcc taaaggcatc aataagtcag
    13261 gtagccatgc tcctgctgcc agaatgacct gatcaaaata atgaccagca atctcataac
    13321 cacttccttg ctgtttcaga gtgacttttt ggcgaataac tggataacca ctagtcttga
    13381 gtaacgtctc acataaagcg gctccctcca ctctagcagc tcctgaagcg tacagacagt
    13441 tatcaaagcc aatcaatcct ttaaaatcat cccccagttc tttacgattt ttaatggcta
    13501 actcaccaat aatcggtgat tctacctttc ttgctaacgc taattcataa agatctctta
    13561 gtttttcttc ctgctttttt aaaacataga tgccattttg ctgataaaaa tctgttgcaa
    13621 aaccatcttc tttgaggtca ttgattaatt gctgataaaa gtccgctcct aaacgagcca
    13681 tgcggtacca aactttattg cgacgctttg aaaaccaagg actaataata ccagcagcag
    13741 ccttagtggc ttgtccttga ccatggtcaa aaatggtaac ttctttttga ccactttgtt
    13801 gtaaataata agcggcggtt gatcctacaa tccccgcccc aataatggct attttcattc
    13861 ctggtatcct tttatcttac tttaagacct taacagccta taaatggcta aatggattgg
    13921 tcgaggcttt gcttgaaata atgctgacat cccaaccgtt ctcttctttc cacccttgga
    13981 gtttctcctt taatttctcc gtaaaaagca cctcaatatg atgccccggg tcaaccgcaa
    14041 atagtccttc tgtcagcatc tcctgtgctg tatggtaata gatatcccct gtaatataaa
    14101 cgtctgcgcc tttttgcaca gcatcctgat aaaattcacc accacttccg ccacagatag
    14161 caatctttga aatcaaaggg ttttccttat cgtaacggat aagtcgcacg gtatctaaat
    14221 caaacaccct tttgacctta cttgctaact cttctaacgc ttgttcttta actgttccaa
    14281 tacgaccaat gccaaagcct tctttggttt cagacaagta agttgcttcc ttgatttcta
    14341 gcaggtcaca aaaccaatca tttagcccgc ctggtacaat gtcaatattg gtatggctca
    14401 catagacaga aatgtcatgc ttgaccaaat ctagaaggat atcacgctga ggggatgaaa
    14461 ccagatcttt aagcggttta aagatgggag catgcttagt tataatgaga tcaacttcat
    14521 ttttgatggc ttctgctact gttgactctc ggatatctaa agtaatcata accttacgaa
    14581 tgtctttatc caatgaaccc atttgaagac ctttaacgtc tctttccata gataaatcaa
    14641 gtgggcaaaa ggcttcatag gcatcaatta gggttttagc tttcatgaac aatcacctct
    14701 tttatttgtt gtatttttgt caagagaagc tgacgctctt gagttttttc ttctgggatg
    14761 caggacaagg cataagctaa cttatccatt tctcgttgcc acttctcttt aaaaacgact
    14821 gatttttcct gagaaaggta tggaccaaaa cgcaattctg tcgcggataa ggccttttca
    14881 ccatgctcag caacgatgat ttcataatat ttgtcatttt cagccatgat ggtttctgca
    14941 acaattttaa aagcgttaac cgataaccaa gcccgtaaat catcctcacg attgttgggc
    15001 tgtaaaacca gacgttcaat gccctgcaat ttttccttac ctgcctctaa aatatcagta
    15061 atcaaacggc cacccatgcc acaaatggta atggctgtca catcatcagc ttcttcaaag
    15121 gctgcaaggc cattggctaa gcgcacctga atatgctcgg caaggccaga ctgtgtgaca
    15181 tttttaagag cagattcgta gggaccccta accacttcgc cagcaatggc tgctgaaatc
    15241 tgattagttt ctaccaaaaa tatgggaaga taggcatgat cactgccaac atctaataat
    15301 ttgacacctt ttggaacata ggccgcaact tgggctaaac ggtttgataa ttgactatcc
    15361 atcttgattt ctttctaaaa cgtaatggtt ctattatagc aaaattacct ccataaaaaa
    15421 agaaaaccaa aggctaggct ttcgattttc cataagtatt ttgatagtac ttacagtaag
    15481 actgaacggg acaaatctca cattttggtt ttttggctaa acaatggtag cgtccaaaga
    15541 aaatcaatcg atggtgagta ataatccaat ctttctttgg aattttagcc atcaaatctg
    15601 cttcaatctg cttgacatcg gcatctggtg atgaaatatt gagcctcttt gagactctag
    15661 ccacatgagt atcaaccgca atagctggaa caccataaac ctctgccaat accacattgg
    15721 ctgtttttcg gccaacaccc ggtaaacttt caagctcttt gtgggttttt ggcacttgac
    15781 ccttaaaatc atcacgaata gcctgagcag ttttaatgat atttttggcc ttatttttat
    15841 agagtccaat cgttctcaga gcattttcaa catcagaaag ttcagcaaaa gctaagtctt
    15901 ctatttctgg ataagactgc catagtccag gagttacctt attgacggcc ttatctgtcg
    15961 tttgagctga taaaatcacc gcaattaaca attgaaaagg tgtttcccag tctaactctc
    16021 ccttagcctc aggaaacatt tgaccaatga tggtcaggac ttttgctaat cttgcttttc
    16081 caattctcat gaatcactcc ataaattcat cgcagataga aaatcctctg atatcgtcac
    16141 ttgagaaaga tcttcgcctt ctctaacacg tctacgttct tctacttgtc gaagattaac
    16201 aataccttct tttcgccaat tacgtaagat agcctggata tatttccaat ttgttttgcc
    16261 gttgaagaca gcttctttta aagcttctcg tatcaaatca ggatctgttt tatcatcacg
    16321 aagcgttttt tcaagatctt ctaactcaaa aggactcaag aatcgtccta attcacgttc
    16381 aaattcatca actaatcgtt tgaaatggtt tggtgtttct tgtttgtcaa tttcagttgc
    16441 cgtttgactg acaaacaatt gatctaactt ggctaacaca ggactcgcat caaaaataat
    16501 ttcaatttct ccagtaagtt caattgtccg catatctaaa agcccttgac tagtcaaaga
    16561 tgaaatgatt ttattaatat cagcaactga ttttccaagc gcatgagcaa tttgactggg
    16621 cgctaagtcg tctcgctttg tcgtattttg aagatagaaa aactgccaga ccaaaaaatc
    16681 atctgagctc ttaaaaagat ctttgtaatg aaaaagcaag gcacttggga taaccaaatt
    16741 acctgactta taatgttcta aaaaactcat aatccctcaa tcaaaaggct aaaatcgaat
    16801 ctatagcctt tactacttta aatcccctaa tcagatgctg attaatgtgc ttacccagga
    16861 aactgcatta agactttata gtcatagttc ctaatagcat gacgaccatt taaaccttct
    16921 aattcaataa ggaaggcaca accagctaca ataccaccaa gtttttcaat catttcaata
    16981 gttgctttta ccgtaccgcc agtcgctagt aaatcatcga caatcaaaac acgttgtcca
    17041 ggcttaatag catctgcatg catagttaag gtatcaagac catattcttt ttcgtaatcg
    17101 gcagagacaa catctcttgg cagttttcct ggtttacgaa cgggagcaaa accaattcct
    17161 aactcaactg caacaggaca accaatgata aaaccacgcg cttcaggtcc aacaaccata
    17221 tcaatatctt tatcacaagc gtactgtgct atttcacgaa tagcatagct atatgcttta
    17281 ccgtcagcca ttaaaggtga gatatctcga aacgtaatac cagctttagg gtaatctttg
    17341 atcgatgcaa tgtaatttgt taaatccata gttaattctt tctagtggca aattgccaaa
    17401 tataaaatac tcttcattat atcacgaaac atatttttcg taaaataaga cttctgacat
    17461 tatgtttatc tgatacttca ttcgtaacta cctcaataac tactgtcaac tgctttactt
    17521 attgatcgtt tgatttcacc aaataatcat acatctcttt cgggctagcc aaagccatga
    17581 tttcttgaaa tttcaccaat tcttttaagt cttggtaaat gtggctctct gaaatctctc
    17641 gtttttgcgc ttgggggtta accgtcatca gcccatcgtc aatcgtcaca aaagacaact
    17701 cttcaaaaat ttgaatcaac ttaatcagta aaagtttttc tatgtttaaa tagtgactca
    17761 actctgtcaa tttatgccgt aaatcaaatt caggaaactg atagatggtt ttgtagagct
    17821 tggcaaactg ctctctgctg ccaaatcctg ttaaataata aggatgtttc atctgatttt
    17881 taaaatagat ggcgtcaaaa tccttgtgaa caaactggtt gcgccaggtt ttgaaatctt
    17941 cagggatgtc attaatcaga atgactctgg catttgggtc ttcttcaata gtcggaatac
    18001 cttcaggaac tttggctgtt tttgtcctta agtcaagaag ttgcactccg tcaacacgag
    18061 catctactaa catgaattgg agactcgtgt tcccattcca atggttgaca gataaggtaa
    18121 ctgctaattc taaaccagta gcttgtcgga actcttgcag ttgacttcct tgaccaaaag
    18181 ccagtacgtc aaaggaagct ttgccctgac tgactttaaa tttaagatgg ctctggtctt
    18241 gccctatggt acgcgcttga ctaaccctta tgtccttgac ataaaagacc ggcttttgat
    18301 gatccatccc ataaggggct aatttatcta aactctttaa aatatcaagg ctaaggtcgt
    18361 ccaaagacag tctttcatca atagttaacg tatttttggc agtctgatct aagccacgtt
    18421 ctatcacaaa ctgacaaaga aagtctgaaa gagcttccaa gttgtcaacg ggcagggtca
    18481 ttcctgctgc accagcatgt cccccaaagg ctgtaaataa ctcacgcttg ccattcaagg
    18541 cttcaaaaat attaatagct tctaaacttc tggccgagcc ttttgcaaag ccattatcaa
    18601 tggtcaaaac gacgaccgtt tgtccaatag tttccatgat acgaccagcg acaatcccaa
    18661 gaacaccagg atgccagcca gcttgtgcca agacttgaac aggtttttga ggatcaacca
    18721 tagccatcgc ttgatcaaaa atgtcttgga ctaaggcctt acgctcttca ttttttttct
    18781 taatcatcaa ggcaatcgct tgagcttctt ggtcatcaaa gcccgtcagt agctcaatgg
    18841 ctggattagg gtcatctaag cgccctaaag catttaattg cggagcaatc tgaaagccaa
    18901 tagcgtcttc attaaaatgt tccaaatcaa taggagacaa agacatcaac tcctgcaagc
    18961 ccactctttc ggtgtgtttg agcatggcca gaccattttt gactaaaaca cggttttcac
    19021 ctgtcaagct taccatatcg gcaatggttc caatagctac caagtctaga cagtctgttg
    19081 gtaaactttc tagcaaagct gttgccaatt taaaggcaac gccacagcca gctaagtgtt
    19141 taaagggata atcagcatca ggatgctcag gatgaatgat ggcaaaggct tctggcaatt
    19201 cttcaggcag gctatgatgg tcagttacaa tgacatcaac ttcctgttct tgagcatatc
    19261 taatcgcttc atgtccggcc acgccattat caaccgtgat aattagggac acagcttcct
    19321 gctcaataaa atatttatag acgctctggt tggggccata gccatctgta aagcggttgg
    19381 gaagataaac caaaggctct gctcccatca tgtctagggt ttctttgaca atagaagccg
    19441 acgtcatgcc atcagcatca tagtcgccgt aaatgagaat tcgttcgccc tcttcaatag
    19501 cttgacgaat tcttggcaca gcttttgcca tgtcatgtag caagtaagga tcatgcagtt
    19561 gagacaagtc cgctgttaaa aactcatcta aggcttcttc agtccttatc cctcgctcat
    19621 aaatcaactg agcggcggtc tgggttagac cttttgtctt tgctagctta aagaagccat
    19681 catctggctt cttgtctttt attttccagc tatatttgga tttaatcatt tataattaaa
    19741 tacctttctt gcaagataat cagataattt agggaaaagg gtgtaaaatt gatgggtcac
    19801 cgctaaacta aagggcaaat tcaattctcg tttatttttc ccgataatag aaaccaaacg
    19861 cttagccact tgatttggtt ggagagtaaa tgtaccaacg ctttccaaat aatgtccaga
    19921 cgggtcagct tggtcaaaaa acttggtggc aatgggacct ggattcacgg tggtcacgta
    19981 aaccccttta tccgctaatt ctaagcgaag ggcattggaa aatccgataa gggcaaactt
    20041 ggtggctgaa taaatgctcg atttggctga cgcaatcaag cctgccatgg acacaatatt
    20101 aataaggtga ccttgcccct gctctgccat tttctgacca atcaagcaag caaagtgaat
    20161 gctcgctagg gtgttaacct gaaacatatc agctatttct tgggcagaaa actcttcaaa
    20221 gcctttgaaa gctccgtagc cagcattatt aatcaagaca tcaatacggc catagcgctg
    20281 gtaaatctgg gcgaccattt tctcaatggc tactggattg gtaatatcca actcaaggca
    20341 ttctttgttg tcaatatgct gataacagtg ttctaggcgt tctttgttac gtcctaataa
    20401 aatcaagctg tcttccttgg gtaactgctt aacaattgcc tgagccagtc ctccagaagc
    20461 tcccgtgata acaatgattc tttgtgccat taaataatca cttcctctaa atcttgtacc
    20521 attttaacat tttcaaatat agtggcggca tctttttcca tctggcgaca atcacgccct
    20581 aaaaagcgag ctgatacatg attgagcaag aggcgttttg cgcctgcttc atgagcgatt
    20641 tgtgctgcct gcatgttggt agaatgcccg tgatttctcg caatccgctc gtcaccctta
    20701 ccataggtgg attcatgaac caaaacatct gcatctttag cgagtttaac actggcagac
    20761 gttttgcgcg tatcgccgat aatggtgatg attttgcctt ttttaggggc agaaatataa
    20821 tctttggcac aaatcaggcg gccgtcttcc aactcaacat cttgaccatt tttaattttg
    20881 ccaaagagtg gtccgaaggg aacacccgct gcttttaagg cttctgcgtc tagggttcct
    20941 tctaagtctt tttgaactac tcgataaccc atgcaaaaaa tggtatgcgc cagacgctct
    21001 gcatagacca caaacttatc tgtttccatt attttaccta atgatttgtc atcaaattca
    21061 tgaaaatgaa tctgataagg tacacgcgct ccagaaactt ttagactagt taacacgtaa
    21121 gttttaatgc caattggtcc ataaatatca agatctgtct gttcttcact agcttgaaaa
    21181 gaccgacttg ataaaaatcc tggtaatcca aaaatgtggt ccccatgcaa atgagtaatg
    21241 aaaatctttc ttatttttcg tggcttaatc gtcgtttcta aaatttgtct ttgcgttcct
    21301 tcaccgcaat caaacatcca gacttcattg atttcgtcta gtaatttcaa ggcaagactt
    21361 gacacattgc gctgcttggc tggttgacca gctccagttc ctaaaaattg taattccatt
    21421 caatctattc tttctattgg gtgatgtaaa tcataaattc tcgttgggca aaatcataat
    21481 acttttgacc agaaaatgtt tggctaagag cccataactc ctctacatta aaccccttaa
    21541 gcatggtgat accgtttgcc agacggtaaa tcttcgacaa gagtgtgtca tcaggcgtaa
    21601 tgtcttcaca ttctccttgc tcaaactgct tagcctgcat tttaagctgg cctttgtctt
    21661 cataaaccat atattgtggg aaaatttgtg ttaattcaaa gaagagttcg ggtttcttag
    21721 gctctgcttt ttcagaaaat accaatgcaa cttcttggtc attttggtaa gcaaaaccat
    21781 aagcttttcc agacaaattg aggcgtacaa agggcttttc ttcctcaaaa gatggttggt
    21841 cagtaaaaag ccccaaagcc ttagaaagat taaaataata atcagtcgca gcttgcggtg
    21901 actgtttctg gtagatttca gcaaagtagg cattaataac acttggtggt ggtgtgataa
    21961 aagctagttt ttgctggtct gtcaaactgg ctaatagatg gtttaggtag ttggtatcca
    22021 gactagtaaa tccaccatag gtttttgcta aatcaatata attattcata aaaatcgtca
    22081 agtctccatt gctgttttgg ggaaatgtag cccgaaatat cctctatttc ttggtcaaac
    22141 gtgtagtggt ctaaaagagc aactcggttc aaatcataca gcttgtacgc cttgtcttga
    22201 tgcactttga ttctaaaagg aacaaactgg tctcgaattt ggtcaatgat taaacgtctc
    22261 aagagaattt tgctatcctt tgaacgggct gaaatcctaa tatttggaaa agcagtagct
    22321 gtgaattgtt ctgctatatc caccttgtta taaatagcta aacgtggaat attaagcatg
    22381 tctagctctt ttagaaggtt taaaaccact ttttcttgct cactatgatt agggtcgcta
    22441 gcgtcaatca catgtaacag caaatccaca tatttgcttt cttctagggt tgatttaaaa
    22501 gcagccacta attccgttgg caagtcttga ataaagccta cggtgtctgt taaggttgct
    22561 tgaaattgat tttctaaata gagttgcttt gtagttgcat ctaaggtagc aaataattca
    22621 ttggcttcat agtggctgtc atctgtcaac aagttcataa tggttgactt gccagcattg
    22681 gtgtagccaa tcaagccaat tttaaaggta tctgaaccca cacgacgatc tctaattgtt
    22741 tgacgatttt tctctacctg agttaattgg cgttcaatat cagcaatctg atgacgaatc
    22801 gaacggcgat ttaactctag ttggctttca cctggtccac ggctcccaat accacctgct
    22861 tgacgactta acatgatgcc ctgtccaacc aaacgaggta acatatattt caattgggca
    22921 aggtgtactt gtaatttgcc ctcatggctt ctggcacgca tggcaaaaat atctaagatt
    22981 aactgcatac ggtcaatgac ttttacctct aaaacagctt ctagattagc attttgccta
    23041 gcagtcaacc gattgttcac aataactgca tcaatctcat cagcttccac aatcgcctta
    23101 atctcatcca gtttccctga gccgataaag gttttgctgt cgtaacgttc tcgtttttgg
    23161 ctgaaacttg ccataacttt gacgccagct gttttggcaa gattagctaa ttctgtcatg
    23221 gacatatcaa aatgttctgt tgtctgaagc tcaactccta aaagaatcac tctttcttgc
    23281 tgacgctttg tctcaatcat cacctttacc aatcctccaa attccgtaaa ttctattata
    23341 acatatcttc aacagattct gctaattttg cgtcatgact tctctttctg accaagaaaa
    23401 tccctgaccc tatcatgtac tacctgtggg taatcaggag ccgttatggc agtgaagcta
    23461 acagccattc gattgcggaa ccaagttagc tgtcgcttgg caaaacgtct cgtgttttgt
    23521 tttaactgat cactagcttc tgccaaagtc atttcaccta caaaataggg gaataattcc
    23581 ttataaccta tgccacgact agcctgaaca gtcgggtaat gctcatataa ccattttgct
    23641 tcttccaata agccgttttc aagcatacga tccacgcgct gattgatacg gtcatagatg
    23701 acttggcggt catcattcaa gccgataata agcggctcat aagccgtctc ggcattctct
    23761 aattcatcag caaactgagc caattcaagg gctcggatcg cccgcctgcg attcacctgc
    23821 tcaatggtaa tgttattgac ttgcaagcgt tcatacaagt cgtgatcgtc caattgctcc
    23881 aactcattcc gataggcttt taccgcctct tgatcaactt gacctcctag atgataacct
    23941 tcgagtaaac tttgtaagta aagaccagtt ccacctacaa taattggtaa tttcccacga
    24001 ctaacaatgt ctgaaatagc tttttgagca tcttgaacaa aatcgtaggc cgaataagac
    24061 tccgtcacct cacgaatatc agttaagtga tgaaccgctg cttcttgctc ttcttgagtg
    24121 gccttggctg ttccaatatc caactgtcga taaacttgct ggctatctcc tgaaataatt
    24181 tctccattaa aagccttggc taaactaatc ccaagtgctg tttttccaac cgctgtcgga
    24241 ccaacaatca caactatttt tatttttgtc attttcttgt ctttctatta gaaaagcgtg
    24301 tccaaaaacg ccttttactt gaaaattagc ttatttttcg ctaccatata ttataacata
    24361 agattaagga ccactgaggt ccttcactag aaatggagaa taatcatgga atatcaattt
    24421 gttaaaggtc ttgctactgg cgtcctagca actgctgcta ctgttgctgg tgctgtcttt
    24481 gctgtaaaaa aaacaatcat tgaccctgaa gaagaaaaag ctgctttcat tgaagaaaac
    24541 cgcaaaaaag ctgcgcgtcg tcgtgtagct cgctaacgtg acaatggatc aatttaaaag
    24601 agactagcac attaaagtgc cggcctcttt tttagtaatc tcgataacta gttagtagta
    24661 actgtctctt gatgagccaa gatgttagct gctttactta ttttgaaata gaaatctcag
    24721 gtaagcccca taaacatacg tgactaaagc tgtcgccaac aatacctctc ctagcaaaag
    24781 aacctgccaa cctaaacctt gccaacctaa aaaagataag gtcattttta aggaagtggc
    24841 actaatcaca aacggaaagg taaaagctga aaaacctggg ttaaagggac gattgagtaa
    24901 gcgaggaagc tgtacaacca caaaagcata taacagttgt gagctgatga gaagaaagat
    24961 aaccattcct acttgtggtc taggaaaggt tgctaaataa gacgctgaaa gtaaggacag
    25021 aggggcacaa aacgttgaaa tattgggcat tactgcttga cctaaaccaa tcccataagt
    25081 ctttttggcc ataaaaggta aaatcacagc agttaagaga agacaaaccc aaaaaatcac
    25141 ctgtcctaat aaaaactgcc tactagcagg tgctgttaat gccgccattg caattccaac
    25201 aaaaagcacg gaccaggaag gaaaaacgtt atcccatgaa aaagaaaaga caaaacgata
    25261 ctggtaataa gcaatcagga caagatttcc caaaaagaat agccaccaag tgagaaagcc
    25321 taaccaacac cctcctgtca ctttgagaaa gagtgaagac aacaacatcc ctgtcataaa
    25381 aaaagttgga aaaacagagg caattagagg ttgtcgtaac tgcattttgg tatcgttaag
    25441 gttcctaacc atgccaacaa gcaagatacc ataaataaaa agagctgcca gaattcccaa
    25501 ataactaaaa atcgaaacat aagtagccaa caaattgcca aatgacaacg ttcctaatgc
    25561 caatcctgac atgaccaatg gtgggttttt aaggtgtttc ataagtgtcc ttttctgcct
    25621 attaacagct atttttagtg acataatctt gtttatttta tcacatttcg agcatttttg
    25681 ccataaaggc tgccagatgc ggataattct cgtaagattt tggtgttttt tgagaccata
    25741 gcgccatggc gtaagcataa gcatctgcaa tggtttgttg atctcgataa acatgcccac
    25801 tatccccaat aagaccatct aaatgcgtca tagcaatatc catccgcttg taagaagctt
    25861 gtttgacttt ttcaagcgct tgattgtctt gagaaatagt gtagcgatga gggttgtaca
    25921 ttggcctaaa aacaagggga aaatcacctg ttaagaaatc catgatttcg ttgaatcgac
    25981 tttctgccaa aagattatct tggggaatga gctgagattt cagggtttgt ttcgctataa
    26041 agagcaagat aatacctgcc tgtgtgacga tttgaccatc atcaagctgt aaagcttaaa
    26101 ccattcccaa aggattgatg gtaaggcagt cttcagaatc cagttttacc ttctctatct
    26161 cgtaatacag aaactcacaa ttcttttgaa aaggttctag acagagttgg tgttgacgtc
    26221 ttcgctgttg atggctctgc atgacaaaat gagcttggtg tccacgttca ctagtatctt
    26281 gttgcaaagt ctttgcaatt tggtctcatc atctgtatca caaaaaacaa accagtttcc
    26341 tgatttgttt tgagatatta ggcacctaca aattctggca aggattctcc aatagcttct
    26401 aaacgttctg cccatccttg acgtgataaa ccattttctt gcacatagcg attacgttca
    26461 tgtgcacgac attcaggcga gcatcctcga acatactttg tttcgttttc ttctgaagca
    26521 aaaatttgtt tgttacaaaa cggatttgca caattgacat aacgttcaca aggtgttcca
    26581 tcaaaataat ctttactaat gacagttgga ttaacatgat taattggcac cgagatacga
    26641 tcatcaaaaa catacatcgc accatcccaa agttcacctt gaacttccgg atcttttcca
    26701 tatgttgcaa taccaccgtg taattgtcca acatctttaa agccttctcg aaccatccaa
    26761 cccgagaatt tctcacagcg aacaccaccc gtacagtaaa caacaacacg tttttccata
    26821 aacttatctt tgttgtcacg tacccattgt ggtaactcac ggaaattgcg aatatcagga
    26881 cgaattgctc cacggaagtg acctaagtca tattcataat cattacgcgt atctaaaaca
    26941 acagtatctt catcaagcaa ggcttcttta aattgtttag gatttaaata ctcacctgtt
    27001 gtttccaaag gattaatatc actatcaaag ttattgtctt ctaacccaag gtgcacgatc
    27061 tctttcttat aacgaacaaa cattttacgg aaagcttgtt gattttcctc gtcaatcttg
    27121 aaccaaagat cagcaaaacg ctcatcgctg tggacccaat ccatatactt ctgagttgtt
    27181 tcataatctc cagaaacagt tccattaatc ccctcatccg caatcaaaat acgaccttta
    27241 agcccaatag atttacaaaa ttctagatgt ttcgcagcgt actcttgcgc attttcaatt
    27301 gaaacatatt tataatagag taaaactctg attttttctg acattttttc tccttcattg
    27361 ttattcagtt acttcctatt atatctagga aaaacaagtc taggcaagtc atttgtttgt
    27421 cttttttttc acaaaataga tctcaattta tttttgatga gatgagatgc aacccattac
    27481 tcctttaagc agatcattag aagctttatt tagaaaatgc taagataaaa caaggaggcc
    27541 aaaatgactt atgaactttg tttagaatat gggacttatc cgttgtcacg tgtttatgct
    27601 tattggggag aagatcaaaa tcccccaaca ttcattcaag aagatcgact gctttgccat
    27661 aaactagaaa ctatgaatca cctgttccat gatttatttg taactattga atcacaattc
    27721 cattatgttg gttttaacat gcctgaaaaa agagcgcaga ttcggatact atatcaagaa
    27781 gtagctacta tattaaaatc caaatataag gattatccaa ttaagattga gacgtttctt
    27841 ctttaaaaat ctaatactaa aaaactaact gtgcttagac agatgacaca gctagttttt
    27901 taggtaaaac tcttcttaaa ttttaaaacg tttcgaaaaa tcactaaatg ctttaatagc
    27961 acttgctaaa aatggtttca ccatctcatc aataattaac cttgattact tacaatctta
    28021 tgaatctgag tcaaataaaa ttcaggctgt ggcattacag agatatttaa aacctatact
    28081 catcgttttc aaggtcagaa aaataatgat tttgaaaagg ttcgtcaact cttctagata
    28141 attttggaca aattttggaa aagaaacaaa aaaagcctat caaatagggc ttttttatat
    28201 gatttctatc caccttgagg gaatcgaacc cccatctcaa gaaccggaat cttacgtgat
    28261 atccattaca ctaaaggtgg tcacaacttt gtcaataccc ttatagtata tcataaaatg
    28321 aaataatatg aaataacttt tctaaccaga agacaataaa aagttgagga aaacctcaac
    28381 tttttagatt tattacaatt caatatcacc gaaaagatca gccattgaga atccagtttg
    28441 tgtttctggg agttcgtagt cacgtcttga ttcacgtttt ggacgacgtg ggcgtgattg
    28501 acgtttttct tctttgttgt ctccttcagc ttgcgctggg cgttcttcaa gagctttgat
    28561 tgataatgat acgcgctcat cagctgcatt cacttcaaga actttaactg ttacttcttg
    28621 tcctacagaa agtacatctt ttggattttc aacacgtttg tgtgaaattt gtgaaatatg
    28681 aacaagtcca tcaatacctg gtaatacttc aacaaaagca ccgaagtcag tcaagcgttt
    28741 tactttacct tcaacaacat caccttgagc aagtttttgt tcaacgccgt cccatggtcc
    28801 aggtgttgtt gctttaagtg aaagtgacac acgaccagct tcttcgtcaa ttgaaagaac
    28861 tttaacttca acttcttcac caactgaaac aactgattta ggtgacacat tacgttcgtg
    28921 agacaattca gtcacgtgaa caagtccgtc aacaccacca aggtcaatga aagcaccaaa
    28981 acttgttaaa cgcgctacag tacctgtaac gactgcacct tctgaaatct tagagaacac
    29041 ttcagcacga gcttctttag cggcctcttc gataacttca cgacgtgata ggatgaaacg
    29101 gttttctgct gcatcaactt ctttgatttt agcatcaaac tcttgtccaa caaatttttc
    29161 agtgttgcga acaaaacgag tgtcaatcat tgaagcaggg ataaatccac gaagtccttc
    29221 aaattcaact gaaagaccac ctttaacagc acgggtacct ttaacagtaa caacttcgcc
    29281 ttcacgacca acaagtttgt cccaggcttt acgagcttcc aaacgttttt tagatactag
    29341 gaaagtaact gtatcagtat ctttacctac tacttgacga agaacaagta cttcaactgt
    29401 gtcgccagct ttaacaaagt cgttaatatc agcatcgcgg tcgttagtca attcacgaag
    29461 tgtcaagaca ccttcaacac ctgttccttc gataacaacg tttgcttgac cgttatcaac
    29521 agttaaaact tccgcagtga caacatcacc agggttcact tcgctaacac tgtttagcaa
    29581 atcttcaaat tcattcattc taaaaaatcc tccaacaaaa gctagccaat gctagctttt
    29641 gataacaata tattttctca aggtaacccg caacgacaac aactcttatc tttgacggtc
    29701 tcagggagca cctgatacct ctgactgggg tagctggatt cgaaccaacg catgagggag
    29761 tcaaagtccc ttgccttacc gcttggctat accccaaaaa tattttttac agaaaactaa
    29821 ttttctaatt agattagttt ccgaaatgga aagagaggga ttcgaacccc cgaacccgaa
    29881 ggagcggatt tacagtccgc cgcgtttagc ctcttcgcta tctttccgta acaacaaaaa
    29941 ctattctatc atagtttttg taagctggca agtgtttttg tctaatttta atggttcaaa
    30001 ttgtagtttt caatgatatt ttcaaccgct tgttcgagcg ttttgaagaa gcaatctact
    30061 ccatcatttt ttaagacagc aaatttctca tcaagttctg caatttctcc aaccacacgt
    30121 ttaccaattt gtaattgata gccatcaatc ttagtttcat taaccataac tttgtgatca
    30181 atcagttgga tttcaatttt tttatctttt ttactcatca ctttacctca ctttgtctat
    30241 tttacacaaa atcaaaaaag cttgcaagag caagctctat tttaatctac ttttacaatc
    30301 caaccttctg gtgcttcaat atccccaaat tgaataccga ccaactcatc gtataaacgg
    30361 cgtgtcacag gaccaacttc tgtttcactg taaaagacat gtaagttatc cttgtactgg
    30421 ataccaccaa ttggagagat aacagctgct gttccacaag ctcctgcttc cacaaattta
    30481 tccaattcat taattggaac atcaccctca attactgtca taccaagtct ttcttcagct
    30541 agttgtaaga gtgaatactt cgtaatagat ggcaagatag atggactaag tggcgtaaca
    30601 aattcattat ttgcagtaat gccaaaaaag tttgcagctc cgacttcttc aattttagta
    30661 tgggttgctg gatctaagta aatcacatca gaaaagcctg ctgatttagc ggccttacct
    30721 ggtagcaaac ttcctgcata gtttcctccc accttagcag cacctgttcc gtaaggagcg
    30781 gcgcggtcaa acgcttcaga aacaataaaa ttagtcggag caagtccacc tttgaaatag
    30841 tttcctactg gcatggcaaa aatggtgaaa atatattctt cggctggttt aaccccaata
    30901 atatctccaa ctccaattaa taacggtctc aggtaaagcg ttgcacctgt tccgtaaggt
    30961 ggtacaaagt cttcattggc tttaaccact tgcttagctg catcaataaa ttgctctgtt
    31021 gatacttgtg gcatcaacaa gcggtctgct gtggcttgta aacgcactgc attacggtct
    31081 ggtctaaata gttgaataga gccatctttg gtgcgataag cctttaaacc ttcaaaagct
    31141 tgttgcccat aatgaagcgc tggagcgctt tctgagatat gtaaggtggc atcttctgtt
    31201 aattgacctt tatcccattg accatttttg taataagaaa tgtaacgaaa tggcaactta
    31261 tgatattcaa atcctaggtt gtcccaatca attgctattg tcatcatcgt acctcttttc
    31321 tgcttttttc tattgtaact ttttttcaat aattttcaag tttattctac tcttttgcat
    31381 taatattcta aatttaccat aaaattaaaa agagttttca ctctttttaa cgtgttctag
    31441 ctaccagtac ttcttgatcc gaaatagtgt ctgaaataaa ggagccattg ctggttcttt
    31501 ccgataacga aagcgtttct aaagcaatct cgtaggtttg accagtctta ctaatgactt
    31561 caagcatttg ttgattagtt tcttcttcag gtatatcagt gaacaaatca aactgctcag
    31621 ctgaagtatc tgattgaacg cctccggcca gaaaaactct atgaggtttg gtttttaact
    31681 ctcgtaggac caatagtcca cgattggcac gacttgtctg aggaatatca tcgacagcca
    31741 tccgtttgag agaacccctc tgagttaaga caaagaaaga gttacttgtc acagcaaagg
    31801 ctgaggcaag gctgtcatca tttttcaagt taatgccttt aacaccagca gatttcaacc
    31861 cttgaatagg cacttcctga ctggagaatc gcaaagcata accattttta gtcactaata
    31921 ggaggtcttc cataataact ggtgacaagg taaccacgcg gtctttgtca tcctttagct
    31981 tcacatattt ggttgattta gatttataag ttcgccaagg acttagttct ttacgatcaa
    32041 agcgtttgat aaaaccattt tgtgtgacag ccacatacaa gccctggtca aaggaagtca
    32101 caatatcagc gtagagaatc tcttcttctg tcgcgaagtt tgaaatggtt tgtgataaat
    32161 gttcaccgat atccttccaa cgtaaatcgg tcaattcatg gattggccgg taaatgacat
    32221 tgcctaaagt tgtaaacaac aaaaggtgtt ctgttgtttt ggcctgttta acgaagatga
    32281 gttcgtcatc atctctcttg ccaacttctt ccaaactaga cgcattgaag gatcgtgggc
    32341 tggttctttt gaggtatccg ccccgtgtca cactaacaaa agtttcttcc tctgcaatca
    32401 agctggcagt gtcaatctca atgatttgac tttctgcttg taactctgat aaacgaggat
    32461 tggcgaattt ctttttcact tctcttaatt cacgtttcat gacgttatac atggttgctt
    32521 cgtcaccgat aatagcggaa agggtcgtga ttaagtctct taaatcattt tcttcatttt
    32581 ggagagtaac aatatcggta ttagttaaac gataaagctg caaggttaca atcgcttctg
    32641 cttgttcctc agagaaatca tagctgactt tcagattttc tttagcatca gtcttattgt
    32701 cagacgatct aatcaaagca ataatctcat ctaagatgga caagacacga atcaatcctt
    32761 caacaatgtg gaggcgtttt tcagctttgg ctttatcgaa tttagaccgc tcaataataa
    32821 tatctttgcg gtgactgata tagcttgata gtattttttg aagccctact tgacgtggtg
    32881 tgaagtgatc aatggcaacc atattgaaat tataattaac ttgaaggtct gtgtatttca
    32941 acaaataatt tagaatggtt tggctatctg catctttttt caactcaata gctatgcgaa
    33001 gacctgtgcg atcagattca tcacgtactt ctacgatgcc tgggacttta ttgttaacac
    33061 gaacatcgtc aatctttttg accaagactg ctttgttaac ttcataagga atctcagtga
    33121 caataatttg ttgtttacca cctttaagtt cctcaatttc cgtcctagag cgaacaacga
    33181 cacgtccctt acctgtttcg taggcttttt tgatttcgtc agctccttgg ataatgcctc
    33241 ctgtcgggaa atctggccca ggcaaaaatt ccatcaattt ctctaagcta gccttgggat
    33301 gatcaatcat atacacaaca gcatcaatca cttctgacaa attgtgcggt ggaatatctg
    33361 tcgcatagcc tgcggaaata ccagaagacc cattgacaag taagttagga aaagctgctg
    33421 gtaaaacggt gggttctttt tctgtgtcat caaagttcca cgcaaaagag accgtatttt
    33481 tttcaatgtc ttgaagaaga taacctgcaa tttcagacaa gcgagcttct gtataacgca
    33541 tagccgcagg cggatcgcca tccattgaac cattatttcc gtgcatttca acaaggattt
    33601 cacggttttt ccaattttga ctcatccgta ccatggcatc ataaatagag gaatccccat
    33661 gcgggtggaa attccccatg atattaccaa ctgatttggc tgatttacgg taaccttttt
    33721 caaaagtatt gccatcctta ttcatcgaat aaagaatccg acgctgaacg ggtttcaatc
    33781 catctcgaat atcaggaagt gcccgttcct gaataatata cttggaataa cggccgaagc
    33841 gttctcccat aatatcctca agggacatgt tttgaatgtt tgacatattc aaatagcttt
    33901 ctttttcatt atttattaat tacctaaaaa acaatcgtct ttagcacttg taattatctg
    33961 tgagaaactt gtatgactaa aacaccgtat tttcttctaa ggtaaacttg acgttatcct
    34021 cgatccactg acggcgtggg gctgctttgt cacccatcaa cacagaaaca cgtcgctctg
    34081 ctcttgccaa gtcgtcaata gtaacacgaa taagggttct ggtttcaggg tccatagtgg
    34141 tttcccataa ttggttagca ttcatttcac ccagtccttt atagcgttga agaatagctc
    34201 ctttaccaaa ttccttacgt aaatcttcta attcaccatc tgtccaagcg taggacactt
    34261 tctctgtttt acctttacct tttgacattt tataaagcgg gggaagcgca atataaacgt
    34321 gacctgcttc caccaacggt cgcatataac ggtaaaagaa agtcagcaaa agagtctgaa
    34381 tatgagcccc atcggtatca gcatcggtca tgataataat cttatcatag ttgatatcct
    34441 cgaggttaaa gtcagctccc actccagctc caatggtata aaccatagtg ttaatttctt
    34501 catttttcaa gatgtcagcc atcttagctt tttcggtatt taaaactttg ccacgtaatg
    34561 gtaaaatagc ctgaaatttg cggtctcgtc cttgtttggc tgaacctccc gcagaatctc
    34621 cttcgaccag atacaattca tttttcttgg catttttgga ctgggctggg gttaattttc
    34681 ccgacaacaa gcctttgtct tttttgtttt tcttaccatt acgtgagtca tcacgcgctt
    34741 ttcgagcagc ttcgcgagca tctctagctt taatggcttt gcgaactaga tgagaagcta
    34801 cttctccatt ttcgagtaaa aagaaggtta atttttcaga aacaatgctc tctacaatag
    34861 gtctcgctaa gggacttccc aatttgtctt tggtctgtcc ttcaaattgc aagtgctgct
    34921 caggaactaa gatagataat actgctgaga gtccttctcg ataatctgag ccctctagat
    34981 ttttatcctt ttcttttaag agattggttt tacgagcata gtcattcatc gctttggtaa
    35041 tagctgactt tagtcccgtt tcatggcttc caccatcttt ggtgcgcaca ttgtttacaa
    35101 aggataagat attgtcggag aaaccatcgt tatactgcaa agcaacctcg acttgaaagt
    35161 cttggtcttg accttccatg taaataacag gtgtcaaggt ttccttatct tcattgaggt
    35221 attcgacgaa atcttgaacc ccattttcat aatgaaactc ttcaataatc gggtcatctc
    35281 cacgaagatc tgtcaagctc atcttgacat ttttcaaaag aaaagcagat tctttcaatc
    35341 gttctgaaat ggtattgaac ttaaagtcaa tagttgaaaa aatcttatca tctggcataa
    35401 aggttacaac cgttcctgat ttagacttag gagctgtgcc aactttttta agggttgtaa
    35461 caggtttccc cccattttca aagcgctgac gatacactga cccgtctctt gtgatttcga
    35521 cttctaacca ggcagacaaa gcattgacaa cagaggagcc aacaccgtgc aaaccacctg
    35581 acgttttgta gccaccctga ccaaatttac cacctgcatg aagaatcgta aaaataactt
    35641 gaacggtggg aatccccatg gcatgctgac ctgttggcat tccacgtccg ctatctgcca
    35701 cactgaccga gccatcttta ttgataacaa ccttaatatc atctccaaaa ccagacaagg
    35761 cctcatcgac agcattatca acaatttccc aaatcaggtg gtgcaatccc gtagcatctg
    35821 ttgaaccgat atacatccct ggacgcttgc gaacagcatc taacccttct aggacttgga
    35881 tagcatcgtc attataatta ttaattgtaa tttctttctt agtcaacacc gacctccaga
    35941 cagtttcatc ttttttatct tacaagtttt ttaccaattt tgcaaaataa tttgctaatt
    36001 ttttccgaat tcttttccag atctagttct ccaatctatg aatatgttat aataaggatt
    36061 atgaaattac tactttttat taccattgcc tatttacttg gttctattcc aactggacta
    36121 tggattggac agtactttta ccacatcaac ttacgagagc atggatcagg aaatactgga
    36181 accacaaata cttttcggat tttaggtgtc aaggcaggaa cagctacctt agctattgat
    36241 atgtttaaag ggacactttc aatattgtta ccaattattt ttggtatgac ttcaatttca
    36301 tccattgcta tcggcttttt cgcagtttta gggcatactt ttcctatttt tgccaacttt
    36361 aaaggtggta aggccgtagc aacaagtgct ggtgtattgc taggctttgc tccgttatat
    36421 ctcttttttt tagcatctat ctttgtttta gttctctatt tatttagcat gatatcttta
    36481 gctagtgtgg tttcagctat cgttggtgtg ttatctgttt taacatttcc tgccattcat
    36541 tttcttttac caaattatga ctacttttta acttttattg tgatattact tgcgttcatt
    36601 attattataa gacacaaaga taacattagt cgtatcaaac atcatactga aaatctaata
    36661 ccttgggggc taaatttaag caaacaagtg cctaaaaaat aaatttacta aaagcctgac
    36721 ttcctctgaa agtcaagctt ttttgtttta actcatttgt taattagggt aaacaacctc
    36781 tccgtctgca atagtgtatt tcacgacacc tttgagtttg ttaccaataa atggggaatt
    36841 actagcttta gaagcaaaat tctcagtgat cagacgttct tgcttgtctg caaaaataac
    36901 caaatctgct ggaccatttt ctgccaaata acccgcatca aaaccataaa gcaaagctgg
    36961 attaagcgtc attttttcta atagtgacat aagtgtaaga tgacctggtt caaccaaatg
    37021 cgtcaaacct aatgacaaag aagtttcaag tcctgtcata ccagatggag ctttagtcat
    37081 atcatcaaca gctttttcat ccttgtgatg aggagcatga tcagtggcaa tgactgttat
    37141 aacacccgat ttcaagcctt ctatgacagc taagcgatct ctttgtgtac gaaggggagg
    37201 attcatcttg gcgctggtac ctacaatcaa taagagatct tcagttgttg aaaaatgttg
    37261 tggggacact tcagcagtta ccttagcacc caactgctga gcaaaagcca ctacttgaac
    37321 cgattctgct ttagataaat gttggatatg aacatgagct tgcctgtcat aagcaatcat
    37381 gacatctctt gcaatcatac tatattctgc tacaccagtt gccccacaaa aatgaaaatg
    37441 ctcctcagca ataccttcat taaaaccaag aaccccattt aactgaggat cttcctcatg
    37501 caaagaaatg aaagtttggt tggcattagc caaatcaaag gcttctttta aaactttaga
    37561 actttctaag ggaataccat catctgaaaa gcttactgct cctgcttcca acaaagcttt
    37621 aaaatcagtc acgtcttttc cattaaaggc ttgggtgaca ctggcattgg tgtaaatatg
    37681 aatcttttct ttagctgcgc tagctaatac ttcctgtaaa gtttcgacat ctgaaatgac
    37741 aggattggta ttagccatca tgacaaccgt agtcacacca cctgatgctg ctgccaaggc
    37801 tcccgtgtga atatcttctt tgtgcgtttg accgggttct ctaaaatgca catgaatatc
    37861 gaccaaaccc ggagcaacaa taagaccact agcatcaatc acttgagctt cttggcattc
    37921 aatagctgaa gctatcttta caatctgttt accatcaatc aagacatctg ccacttgatc
    37981 tctctgcgat ttcgggtcca taacgcgtcc atttttgatg agtaacatgc tctctccttc
    38041 ttctcactta tctgtaaaac tgatagacag acctgtctgc caataatact gaaactttgt
    38101 ccataggctg cttacctcaa accatctgcc tgataatcac taatgcttgt cctattgtaa
    38161 ccaatctatg ccagtcatgc cttctttttc taaaatagca tttgttcttg aaaagggttt
    38221 gctgccaaaa aagccacgat aagacgacaa agggcttgga tgaggactct cgatgatgtg
    38281 gtgctttgga ttagtaatga aggctttttt cttccttgca taagctcccc acaaaatgaa
    38341 aactacaggg ctgtctttct cattcagcac tttaataaca gcgtctgtaa atggctccca
    38401 tattaagcct gcatgcccat tagcctggcc tgctggcact gttaagcaag cattcaaaag
    38461 cagaacccct tgactagccc aagtgcttaa atcatgatgg tcacgaggac caatgtcatc
    38521 tgctaattct tttaaaatat taataaggga tggcggagca gaaatctctt ctggaactga
    38581 aaatgataag ccctgggcct gtttggggcc atgataaggg tcttgtccta aaatcaacac
    38641 tttggtttct tccaaaggag tgacttgtaa ggctttaaag acattttctc gtggaggata
    38701 gacaagaccg gaagcatagg cttcatctaa aaagtgattg atgcgaccgt agtaatgctc
    38761 cggcaaaaat gatttgattt tctcatgcca aattgaatga gccataagat tcctttctaa
    38821 tcacgtcagt ttctaagtta aatacgatgt ccttttctat ctagtttacc acattttaaa
    38881 tagaaagacc ctgaaaatat gatatttcca gggttaatat tcgtttttta caggatatta
    38941 aacaagtgct agcttatttt ctaagaattc ttggtaacgt tctaacaaaa aagcaataat
    39001 ccaatagata aaggctacta aaatgtacat ggacatgtag tcccactccc taccaccgat
    39061 aattttggca ttttgaaaaa tatctggtac ggtaatcatg gcagctagcg acgaactttt
    39121 aaccatatca ataatgacat tgcctagagg aggaactgca attcgaaagg cttgaggaag
    39181 aataattttc ttaaccataa gagcatatgg caaccctaaa gccttagctg cttcccactg
    39241 tcccttgtca atagcttcta tagaagagcg aaaaacttca gaaatatagg cagcgctaac
    39301 catagaaaaa ccaagataag cgcaaagtaa agctggcaat tctaaaccat aataaggcaa
    39361 cccgaaataa agaacaaaga gcaccacgat catcggcaca cctcgcataa tagagatgta
    39421 ggccctaaca agatagtgta gcagtggctg atgtgacctt cctagtaggg caaggagcaa
    39481 accaaagacc agccccgtta aaaagctaag caacgatatg ccaagggtat aaggtaatcc
    39541 cgataataca aatcctaaac tatctttcat taatgggata ttaatcatgt ggttaggact
    39601 cctttaacat caagtattag ttaacgtctt tcgtatcaat gatgggaatt tttttgtctt
    39661 tgccaaaagg ttttgtcaaa tcttgaccag catagtaggt ttcagatatt ttcttcaagc
    39721 taccatcttt tatcatgtct ttaataacag catctatttt tttcttgagg ctatcgtctt
    39781 ttttgttcat aacaattcct tgttcggttg ggttgtactg agcatcgctc attttgacat
    39841 ttagatttgg attttgagat aacatgtaat ccacaaataa tttttgagct gggtaatcat
    39901 ttggaatgaa atctgttcga ccgttagcca catcgtttag ataaacatct cctgtaacat
    39961 tatcgtaggt caccagttca gctccctgtt tttgcgctac tttcatgtac tctgttccag
    40021 aagcgccagc tgcttttttt cctttccagt cgctaagatc ttttttagcc atgtttgagc
    40081 tgccatcagc acggacaatc atgcctccta ccccgtattt ataaggcgta gagatattgt
    40141 attttttctg acgttttggg gtaatgtcaa agttgttcaa agaaatatcc actcgaccac
    40201 tatcaactga ggtaaaggct tggtcaaatc ctgtttcaac aaacttgact ttaatgttaa
    40261 gtcgtttacc gatttccttg accatatcta cttcgtaacc tactaactca ttattatcgt
    40321 tgtaaaaaga agttggctga taggttcctg gggtagctac ttttaacacc cctttttctt
    40381 tgatagtctc ccaacttgat ttttgagatt tatgtggcgc acatgctgcc aataaaaggc
    40441 tggcaagtcc gatagacagt aaagtaactg atttcttcat agtaactcct ttattaagat
    40501 aagaatagta taacattttg attaagttgt gttatcagtg agcgctcaat ctctttaaaa
    40561 agttaatcta taatagttaa actgttttat aagattatta ttgaggaatc ttcaataatc
    40621 ctgctgcctc aaactgctct gcaattccta ataataggtc ctctctacct ttggctgcaa
    40681 ttagttgaat gcccattggg agcccttcct tagtttcgta agtaggtaaa ctaatagctg
    40741 gctggccagt tagattaggc agagctgtgt aaggattaat agccagactc ttaccaaaca
    40801 tggtttcgac caaattgaat tgctcttcac tgctgaaaat ttcagcttga gccaagttag
    40861 ccatgagttt cgaatctggg acaagttctc cgtgcttagg agctggtgtg ttggttgtga
    40921 acgtcaacaa caggtcataa gtctcatgaa aactagccat ggttgcactg tacgtatccc
    40981 atttttgaag gacctgtgag taacgccaag ctgggatgtc tttacctgat tggtaaatag
    41041 cccaggtcat ggtttccata tcgtctttgg tcatcggacg tccaaaagtg tcttcaatat
    41101 cagcaaacat agcagccgtt tccacactat tcataatata ataatgacga atcacttctg
    41161 tcatatttac aggaaactct tccaattcaa ctagctgatg cccttgttct cttaaccaag
    41221 ttactgcttg tcttaaagct ttttcagtat ctaaagatac tgggctacca tcacttaacc
    41281 gttgataaaa agcaattgtc aagggacgtt gtagcgattg ataaatacta ttttttgtta
    41341 aggtggctag ggggaagggg ctttccattt gttccatctg taaatagtag agtagatttc
    41401 tagtatctct aacggatttg gtcaaggcaa aatgaactga ggctccttgc cagcttcgat
    41461 aagaaccagg tccgacaggc atgcgcccgc ggctaggttt gagcccaatt aagccattaa
    41521 acgaggctgg aattcgaata gaaccaccac catcactagc agttgctagt gcactaattc
    41581 ctgatgacac tagagcagca gctcccccag atgagccacc agcatttcgt gtattatcat
    41641 gaggtaatga aaccgggcca tggagctgac tatcactaat atttttaaag ccaaattcag
    41701 gtgtgttaga acgccctaaa ataataaatc ccaagtcttc taaccttttc acgaataaat
    41761 ctgttttagt cgcatgatga ttcttgaata gcttagaacc cgatgttgat aagtctccct
    41821 tcaattcttg tcccaaatct ttcaaaaaaa tgggaactcc tgcaaaaagc ttacctgaaa
    41881 aatccctttg tttggcttct tctagggctg cttcaaagcg ttctgaggta atagcattta
    41941 aggtagggtt caacttttta gccttataaa tagcctgggt tactaattct agtggtgtgg
    42001 tctgaccggt ctgaacagca atagccatag ccgtcgcatc ttgataagtc atagctactc
    42061 ttcctttcta agatatgaat cctaaaacat tcttttttat taaaacacaa ataaaagaaa
    42121 accaccatta aaaggttaga aattagaatg tgatgtgtaa ggaatgtaga caaaacaatc
    42181 aaaacataac attttgaatt ggggttaaag agattttttc attaaaaata aacctggctg
    42241 atagttcctt attgttgcca gtttacctgg tcttctttaa attttttaag taaatgtaaa
    42301 ccatcattag taatatagcc ttgcaacttt gcaactgcaa ttagctcagt ataattagaa
    42361 agcgtaatca acttgatacc agcttcttta aaattctgac tagcttttgg taactcataa
    42421 gtgaaaatcg caacaactcc cagaacgtca gctccctcac gactggctgc tgcggctgca
    42481 tctagtaccg aaccacctgt tgaaatcaaa tcttcaatga taaccatttt ttggccttta
    42541 agcacacgcc cctcaatctg gttgcctgct ccatgatctt ttggcttact gcgaatataa
    42601 gcaaaaggaa gagtcatttt atctgctata atggcaccgt gaggaatacc tgctgtagct
    42661 gtaccagcga tgacctcaac ttctgggaaa tgagccctga tagtttccac aaacccattc
    42721 tcaattaaat cacgagtctt aggataagaa agggtgacac gattatcagt gtaaataggt
    42781 gatttaatgc cagatgccca cgtaaaagga tcttcaggct ttaaataaac agctttaata
    42841 tcaagtaatt gagttgcaat ttggcttcct agtgtcattt cttattctcc tttttcttct
    42901 ttaacagaca agtgttggtt tcctaatctt atctgttagc ctgcccactc agcttttatg
    42961 gcttggtaag cagccactgg gtcttttgcc tgggtaattg gtcgccctac cacaatataa
    43021 tcacttccaa tgcgtcttgc ttggtttgga gtcatcactc gtttttgatc gccaatatta
    43081 ctgcctttag gacgaatgcc aggagttaag caagtaaatc ccgtaggcgt caccgccttg
    43141 atagcttcta cttcttgtgc cgaacaaacc accccatcta gctgagcttt agctgccccc
    43201 ttgctgtaat gcaaaacaga ctctaaaaga ctcgtttgaa tattttgatc accacgcatt
    43261 tggtcttcac tggttgaagt tagctgcgtt accgcaatta aggtagggcc ttggcctagt
    43321 ccttctcgag ctgcttttag catctctaca ccaccagctg catgaacagt tgccatatca
    43381 atatctaatt ctttaagaac tgccatagca gcacgaacag tatttggaat atcatgtaat
    43441 tttaaatcca aaaatacatt atgccctaac gatttgatag accgaacaat atctggtcct
    43501 tgcgcataat ataattccat accaattttc acgtatagtt tttcttctgc tggaaataag
    43561 tctaaaaatg ctttagtctc ctcaaatgat gaaaagtcta aagcaataat tgggcgttct
    43621 tctttcataa cttatccttt ctataatgtg tcaatatctc attaagcacc aaaaagcctc
    43681 gctaccaaag aagcgaggct acagcaaact aaaagggaat tccctttttt tatcttgtat
    43741 accttgtttg cctctctgga ctcacttaaa ggtgtctaaa tttaaactat tatagctttt
    43801 ttaaaacaag atgtcaaaac tttttgttat ttgaacacat ttttcctact atatcttttt
    43861 ttgtacactt acgtcaactc attttatcat ttttacccaa tcatttattc aaactcacct
    43921 gtgggtacct tagggctttg gtaatatttt ataaaagctt cttcttcaag attaaccctc
    43981 tggatatgtt catagagcac atccggaata ggagcttcct taagtgacaa agcactcact
    44041 tttaaagatg aaaaaacatc acttaagacc ccaaaaactc gaatgcgttt atctccaata
    44101 ataatttgaa agacttcttt ggactggcat aaatctatca gatgatccac tcctttaatc
    44161 caattatcga caatatccaa agttttgttc atcaacttaa aagctgattc ttcataccaa
    44221 aaggtagact caatcaagcg ttgctgcata tccaaggtat caatggccgc aacaatatct
    44281 cccaactcag actgtactaa atgagctgga aattcccctc caagtataga aggagttcgt
    44341 tctacaagct tcagttcatc tcgaaaaaga gtcaaatagt ggatactgac caataacttt
    44401 tggattaaga tttgatgact attttgttca gtaacattca cacgaccacc accctttgat
    44461 tagaaaaata cctatttgtt aatactatta atataacaca agaaaaataa aagggcaatg
    44521 aaattgatag attcaactct caaaattgaa tcatatttat cctaggccat atcgagaaaa
    44581 gactccagcg ctttttcaaa gttctaaacc catgatgacg tttgtgcttg cgtctttatt
    44641 ttttttgagc tagactgtac ttatgagaat tcaacaactg cattatatta tcaaaattgt
    44701 tgagtgcggc tctatgaatg aagctgctaa gcaattattt atcacacaac caagtctatc
    44761 caatgcggtc aaggatttgg aaatggagat gggcattacc attttcaacc gaaatcctaa
    44821 agggatcacc ttgacaaaag atggggtgga atttttatct tatgctcgcc aaattataga
    44881 gcaaacatca ctgttagaag accgctataa aaaccacaat actggccgag agctctttag
    44941 cgtttcatct caacattatg ccttcgtagt caatgcattt gtctcactcc tcaagcggac
    45001 cgacatgact cggtacgaac tctttttgcg tgagacaaga acttgggaaa tcattgatga
    45061 tgtcaaaaac tttcgatctg aaattggtgt tctctttatc aacgactata accgagatgt
    45121 cttgaccaaa ttatttgatg acaatcattt gactgcctct ccattattta aggcacagcc
    45181 tcatattttt gtcagcaaat caaatcctct agcaactaaa agcctattaa acatggacga
    45241 cttgagagat tttccttacc tgagctatga ccaagggatt cacaactcct tttatttttc
    45301 agaagagatg atgtcccaga tgccccatag caaatcaatt gtggtcagtg accgtgccac
    45361 cctctttaac ttaatgattg gtttagatgg ctacaccgtt gccagtggta ttctaaatag
    45421 caatctaaat ggtgaccaga ttgtggctat cccgctagat gtccctgacg aaattgacat
    45481 tgtttttatc aagcatgaaa aagccaactt gtccaaaatg ggggaacgtt ttatcgaata
    45541 ccttctcgaa gaagtaacct ttgattaatt tctttttcaa tcgtcacact aaaaaacacg
    45601 gtagtaagac ctgtatcggt caagttatcg tgttttttga taaatatcag acaatagatt
    45661 gtctccaaag gcccaactcc gttttaataa tggcattcat gtcatcaaag taaaaaggtt
    45721 cacgtcggta gattacacca gaaaaagtcg ccaaggtgat aatgttttcc aaaaaagcct
    45781 gtgaagaaaa atgagtggtg cgatgcagtt cctcttcatc aaaaggctta atcaaatgag
    45841 ccagaggatt acggacagcc ttttcaaaat gtcgtaacaa aaaaaccaag tccttgattt
    45901 cctgaggtaa ggaggtcaac gtcaacatgt ctgctaagct tttggtcgtc acattgcgag
    45961 actgcttttg agacaggtaa gccttaaaaa cctcgtgatc agattccaac atggcctgaa
    46021 aatgccaggt atcatattgg tcattctttg tatcaaaaat ataagccttg aagtttggaa
    46081 tttctgacgt gattagtctg agaaaaagtc ggtaaagaat aggcgacact gacctgacga
    46141 aatcaataat agcaccgttt tttactttaa cttctaaatc caaaatgtaa ttggcaatct
    46201 gctcatcttc aagcgattgg ttaagccata atgaacagtg gtactggtct tcaatgatcc
    46261 gattctctgc tgcatgttcc gccagaaagg caaggttcaa ttctctgcgc tcttttaaca
    46321 tctctagtaa atagagggca gttttaggga tatttggcaa ggctttagca attctaaagg
    46381 cgtgattata agcatagaca tccaaaagga tgtctagtgt ttgattatta gtcattggca
    46441 attaaggttt ccaaggcgac cttcatcata tcagtgaaag tggtttggcg ctcttcagct
    46501 gtagtatctt cggttggatc attcaagtta tctgagatcg tcatgattcc aagggctttg
    46561 acatggtgct gagcagctaa atagtaaaga gctgctgctt ccatttcgat ggctttaaca
    46621 cccaatttac caagagccat attacgctca ggcatgttag tgtaaaagac atcagaagaa
    46681 agcacattcc caacgtgagt tgttactccc atttctctgg cgatatggta agccttgtca
    46741 agtaaaccaa aatcagcaat ttgtgggaaa tcaaattctg ggaagtcatt acggataata
    46801 tttgagttgg ttgctgctgc ttgggcaagc accaattcac gcacatgaac ttctgggtca
    46861 atcgcacccg cagtaccaac acggatcaat gttttgacac cgtaatcaac gattaattca
    46921 cgagcataga tagagatcga aggcatcccc ataccagttc ccatgacaga aacgcggcgc
    46981 cctttgtaag ttcctgtgta gccaaacatg ttacgcactt cgttaaaaca cactgcatct
    47041 tctaggaaat tttcagcgat aaatttcgcg cgcaaagggt ctccaggtag aagaatttta
    47101 tcagcaatat cacctttttt ggcagaaata tggatagaca ttattaagat attaaaggcg
    47161 acaaagaaca aagtaaaaat aggaaactga cgacgcatcc cagatgctag acagtttatc
    47221 ttttttacac agttctccgc ccgtattcaa ttcaatgaat acagttagcc ctacctttcc
    47281 ttttattttg tagaaggtca gagggcggac agaaagcaac tgaaatttag ggaactaacg
    47341 ccatgtgtga gcacaaaagt tagtttatct agtttccgag ctttccgccc gtattcaatt
    47401 gaatacaacc ccattccttt cataattgat attgaataga agaagagagc taaaaatcga
    47461 gttcaaataa gaaaggacta actctctttc ctttattatt taaaaattaa tacgaagaaa
    47521 tattcgagtc catgcttaat ttaaaccgaa tacgtctctt tcccttcgtt ggttatgtta
    47581 gttagaaaag ctagaatctg agttcaacat tcatggaact cacaccccgt gattattttt
    47641 tgattttttt attgcgaaca gatgtgccaa actgacgaca taaaataaca ttcctgtaat
    47701 aatgctccag ttgattttgt tcgtcctaca actcagctaa aatagcttta acaagacctt
    47761 tgaaatcttc tttaatatgt tcagtcactt caacaacttc ttcgtggttc aattctgatt
    47821 ggaaaccagc tgcaaagtta gtgatggctg aaatgccaag tactttaagt ccagaatgag
    47881 cagccacaat aacttctggc acagttgaca tgccgacagc atctgctcct aatactttga
    47941 aagcacggat ttctgctggt gtctcataag ttggaccagt taagcccata taaacaccat
    48001 cttctagttt gatgttcatt ttttcagcca cttcatgtgc cttattgcga taaaccttag
    48061 tataggcatc agacatatca gggaagcgtg gaccaaactc gtccaagttt tcaccgatta
    48121 gtgggttatt tccggtcata ttgatatggt ctgtgattgc catcaaggta cctgggccgt
    48181 agccgatacc accagctgca tttgtgacaa gaacgccttc acatccaaga gctttca
//
LOCUS       DAUVUY010000004        43880 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.004, whole genome shotgun sequence.
ACCESSION   DAUVUY010000004 DAUVUY010000000
VERSION     DAUVUY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43880
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.004"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            59..307
                     /locus_tag="VOX58_000217"
     CDS             59..307
                     /locus_tag="VOX58_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HES5877493.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            344..955
                     /gene="amaP"
                     /locus_tag="VOX58_000218"
     CDS             344..955
                     /gene="amaP"
                     /locus_tag="VOX58_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HES5877494.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGLTEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            966..1154
                     /locus_tag="VOX58_000219"
     CDS             966..1154
                     /locus_tag="VOX58_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HES5877495.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            1167..1706
                     /locus_tag="VOX58_000220"
     CDS             1167..1706
                     /locus_tag="VOX58_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HES5877496.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1747..1947
                     /locus_tag="VOX58_000221"
     CDS             1747..1947
                     /locus_tag="VOX58_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HES5877497.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            1958..2446
                     /locus_tag="VOX58_000222"
     CDS             1958..2446
                     /locus_tag="VOX58_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HES5877498.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTNAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2609..3250
                     /locus_tag="VOX58_000223"
     CDS             2609..3250
                     /locus_tag="VOX58_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HES5877499.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(3393..3935)
                     /locus_tag="VOX58_000224"
     CDS             complement(3393..3935)
                     /locus_tag="VOX58_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HES5877500.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            4053..4754
                     /locus_tag="VOX58_000225"
     CDS             4053..4754
                     /locus_tag="VOX58_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5877501.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4769..7405
                     /locus_tag="VOX58_000226"
     CDS             4769..7405
                     /locus_tag="VOX58_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HES5877502.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWSKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRVALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSATSLSPYFNLLDS
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNANHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            7497..8093
                     /locus_tag="VOX58_000227"
     CDS             7497..8093
                     /locus_tag="VOX58_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HES5877503.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLVNL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            8104..9060
                     /locus_tag="VOX58_000228"
     CDS             8104..9060
                     /locus_tag="VOX58_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HES5877504.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            9157..10497
                     /locus_tag="VOX58_000229"
     CDS             9157..10497
                     /locus_tag="VOX58_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HES5877505.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEM
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANERVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFVKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALIKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVRRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFAALKDQ"
     gene            10613..11497
                     /gene="truB"
                     /locus_tag="VOX58_000230"
     CDS             10613..11497
                     /gene="truB"
                     /locus_tag="VOX58_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HES5877506.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARTGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALWVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            11526..12458
                     /locus_tag="VOX58_000231"
     CDS             11526..12458
                     /locus_tag="VOX58_000231"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HES5877507.1"
                     /translation="MEIEYIKDYRDINQEDDTVLVLGYFDGLHRGHKALFEKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNCTDSDYLTRNFEGQVYTIKEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            12501..12905
                     /locus_tag="VOX58_000232"
     CDS             12501..12905
                     /locus_tag="VOX58_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HES5877508.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDVDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            12907..13185
                     /locus_tag="VOX58_000233"
     CDS             12907..13185
                     /locus_tag="VOX58_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HES5877509.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            13175..13963
                     /locus_tag="VOX58_000234"
     CDS             13175..13963
                     /locus_tag="VOX58_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HES5877510.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVTLEDQ"
     gene            13966..15276
                     /locus_tag="VOX58_000235"
     CDS             13966..15276
                     /locus_tag="VOX58_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HES5877511.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALTMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
     gene            15415..16281
                     /locus_tag="VOX58_000236"
     CDS             15415..16281
                     /locus_tag="VOX58_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HES5877512.1"
                     /translation="MKMKKKFFLLSLLVLSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            16292..17227
                     /gene="pstC"
                     /locus_tag="VOX58_000237"
     CDS             16292..17227
                     /gene="pstC"
                     /locus_tag="VOX58_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HES5877513.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATCWQTIWRVVLNAARP
                     GIFTAIIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            17217..18104
                     /gene="pstA"
                     /locus_tag="VOX58_000238"
     CDS             17217..18104
                     /gene="pstA"
                     /locus_tag="VOX58_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HES5877514.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGSLALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            18120..18923
                     /gene="pstB"
                     /locus_tag="VOX58_000239"
     CDS             18120..18923
                     /gene="pstB"
                     /locus_tag="VOX58_000239"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HES5877515.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            18936..19694
                     /gene="pstB"
                     /locus_tag="VOX58_000240"
     CDS             18936..19694
                     /gene="pstB"
                     /locus_tag="VOX58_000240"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HES5877516.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            19762..20415
                     /gene="phoU"
                     /locus_tag="VOX58_000241"
     CDS             19762..20415
                     /gene="phoU"
                     /locus_tag="VOX58_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HES5877517.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            20620..23157
                     /locus_tag="VOX58_000242"
     CDS             20620..23157
                     /locus_tag="VOX58_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HES5877518.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQPTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
     gene            23504..24178
                     /locus_tag="VOX58_000243"
     CDS             23504..24178
                     /locus_tag="VOX58_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5877519.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRSVGY
                     ILKNNE"
     gene            24171..25481
                     /locus_tag="VOX58_000244"
     CDS             24171..25481
                     /locus_tag="VOX58_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HES5877520.1"
                     /translation="MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYS
                     SVDSSLVSVSNNASSYANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDII
                     LFSANGTILNAFDAFSNYQNFHLDKRRLGSIETTSLMNFYGQEEKYHTITVRVHIKNY
                     PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEK
                     QKMFVENASHELRTPLAVLQNRLESLFRKPNETILENSEHLASSLDEVRNMRILTTNL
                     LNLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYGKIFYFQNQVNRSLRMDKALLK
                     QLITILFDNAIKYTDKNGIIEIIVKTTDKNLLISVIDNGPGITDEEKKKIFDRFYRVD
                     KARTRQTGGFGLGLALAQQIVMSLKGNITVKDNDPKGSIFEVKL"
     gene            complement(25606..25854)
                     /locus_tag="VOX58_000245"
     CDS             complement(25606..25854)
                     /locus_tag="VOX58_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HES5877521.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(25908..26828)
                     /locus_tag="VOX58_000246"
     CDS             complement(25908..26828)
                     /locus_tag="VOX58_000246"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HES5877522.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            27063..27689
                     /locus_tag="VOX58_000247"
     CDS             27063..27689
                     /locus_tag="VOX58_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HES5877523.1"
                     /translation="MKKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEILRKDKGYYVLRSIKQT"
     gene            28340..28729
                     /locus_tag="VOX58_000248"
     CDS             28340..28729
                     /locus_tag="VOX58_000248"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HES5877524.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            28823..29875
                     /locus_tag="VOX58_000249"
     CDS             28823..29875
                     /locus_tag="VOX58_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HES5877525.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            30014..31546
                     /locus_tag="VOX58_000250"
     CDS             30014..31546
                     /locus_tag="VOX58_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5877526.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVAERVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGIPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNKEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVVHDGKIQGIVLPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            31539..32603
                     /locus_tag="VOX58_000251"
     CDS             31539..32603
                     /locus_tag="VOX58_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5877527.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            32605..33561
                     /locus_tag="VOX58_000252"
     CDS             32605..33561
                     /locus_tag="VOX58_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5877528.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(33779..35497)
                     /locus_tag="VOX58_000253"
     CDS             complement(33779..35497)
                     /locus_tag="VOX58_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HES5877529.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKHGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(35673..36242)
                     /locus_tag="VOX58_000254"
     CDS             complement(35673..36242)
                     /locus_tag="VOX58_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HES5877530.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
     gene            complement(36300..36845)
                     /gene="coaC"
                     /locus_tag="VOX58_000255"
     CDS             complement(36300..36845)
                     /gene="coaC"
                     /locus_tag="VOX58_000255"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HES5877531.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(36838..37530)
                     /gene="coaB"
                     /locus_tag="VOX58_000256"
     CDS             complement(36838..37530)
                     /gene="coaB"
                     /locus_tag="VOX58_000256"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HES5877532.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNKLLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            37711..38562
                     /locus_tag="VOX58_000257"
     CDS             37711..38562
                     /locus_tag="VOX58_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HES5877533.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            38559..39758
                     /locus_tag="VOX58_000258"
     CDS             38559..39758
                     /locus_tag="VOX58_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HES5877534.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVINQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNIAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            39755..40753
                     /locus_tag="VOX58_000259"
     CDS             39755..40753
                     /locus_tag="VOX58_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HES5877535.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEEAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            40795..41127
                     /locus_tag="VOX58_000260"
     CDS             40795..41127
                     /locus_tag="VOX58_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HES5877536.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            41120..41932
                     /locus_tag="VOX58_000261"
     CDS             41120..41932
                     /locus_tag="VOX58_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HES5877537.1"
                     /translation="MPSSFDLLGEMIDLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGRHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKENNCE"
     gene            41925..42806
                     /locus_tag="VOX58_000262"
     CDS             41925..42806
                     /locus_tag="VOX58_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HES5877538.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            42853..43872
                     /locus_tag="VOX58_000263"
     CDS             42853..43872
                     /locus_tag="VOX58_000263"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HES5877539.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
BASE COUNT        13796 a         7265 c         8998 g        13821 t
ORIGIN      
        1 acagctgtta ttcttccttg gacaaaaggt acaggagtat ctaaaaagga gaattatcat
       61 gggacttatt tggactttaa tcgtcggtgc gcttattggt gtgattgctg gagcacttac
      121 taaaaaaggc ggttcaatgg gctggattgc aaacatcgct gctggtctag ttggttcttc
      181 cgttggtcaa gctttacttg gttcatgggg cccttcttta gcaggaatgt ctttgatccc
      241 gtcagttatt ggtgccgtta tcgttgttat gatcacttct tttgtactaa acaaaactaa
      301 caactagttt cctctttatt tagtaagtac ttacaaatag agaatgttaa gtagaaaaga
      361 ggtctgtatg tcaaaattgt taaagattag ttattgtctt gtcggacttg ttctattgtc
      421 tgtttttgga tgggtagttg gaataaccgg aggctatata tatttacctt atagttatcg
      481 ttggctaagt tggggtatgg atagttttcc caatctctta gattcagctt tatcctatta
      541 ttacttctgg acagcccttg tattatttgt tataacattt cttgccttat tagttattat
      601 tttataccca agaatttata ctgaggtcca gttacgccat aaaaataaaa aaggaacact
      661 tttattaaaa aaatcggcta ttgaaagtta tgttgcgacc gctattcaaa ctgcaggttt
      721 gatgccaaac ccaacagtca cagctaaatt atataagcgg aagtttaaga ttatcgttaa
      781 aggacgttta gcttctcgtg tagcagtggc agatcaaatt agtggcgtga aagaagggat
      841 cgaaaaaggt ttaaccgagt tcttcggtat taattatcct gttaatttca aagtttatgt
      901 taaagatatt gctgattctg atcgtaaaca tattaccaga aatcgtgtag aataggagat
      961 ctatcatgga attttatgaa aaattcaagt accctattat tggtggcttg gtggggctaa
     1021 ttattgccat attgctcatg gcttttggtt tattcaaaac gctactagca attattttca
     1081 ttattttagg aatatatggt ggcctgtatg ccaaaaaaac aggtattatt gatcaatttc
     1141 ttaatcgcaa ataaaggaga cttattatga ctgaaactta tattaaaaac acatctaaag
     1201 acttaacttc agcaattcgt ggtcaattaa catatgatga taaagtaatt gaaaaaattg
     1261 ttggtttagc acttgaaaat gttgatgggt tactgggtgt aaatggtggt ttttttgcta
     1321 acctaaaaga taaattagta aacaccgaat ctgtacgtga tggcgtgaac gtggaagttg
     1381 gaaaaaaaca agttgccgtt gatcttgata ttgtcgctga atatcaaaag catgtgccga
     1441 ctatttatga ctctattaaa tcgatcgttg aagaagaagt gaagagaatg accgatctgg
     1501 atgtgattga agttaatgtt aaagtagtcg acattaaaac aaaagaacaa tttgaagctg
     1561 aaaaagtaag tttacaagac aaagtttcag acatggcacg ttcaacatct gaatttacga
     1621 gtcatcaagt tgagaatgtt aaagcttcag tggataacgg tgttgaaaaa cttcaagatc
     1681 aaaaagctga gccacgtgta aaataatcat atgtcgtcaa ttaggtaagc taaaggaggg
     1741 tttattgtgt cagatgaaaa atataatgcg aaattagatc aagctggtgg caagttaaaa
     1801 gaaggatttg gtaagatttc tggagataaa tctttagaaa ctgaaggtaa agtagataaa
     1861 gttactggaa aagttaaaga ggttattgca gacgccaaag atactgtaaa aggattagct
     1921 aaaggcttag ataataaaga taaataaagg ggacatgatg gaaaaaactc aaattaaaag
     1981 cactcttact tatgacgata aggtcatcga aaaaattgtc ggtcatgctt tggaaaacgt
     2041 tggcggatta ctagctgtaa caggtggatt tttctcaaat attaaaaata atttggtgaa
     2101 ttctgagtct gtaactgatg gtgtttcagt tgaagttggt agcaaggaag tagcagttga
     2161 tttagcaatc attgtagaat acggtaaaga tattcctgct attgcagaat ctattaaagc
     2221 tattgtttct caaaacgttg atagtatgac acatttgaaa gttgttgaag tcaatgtgaa
     2281 cgttgttgat attcgtacta aggaagaaca tgaagctgct agcgttactg ttcaagatcg
     2341 tgtcactaat gcagcttcaa gtacttcaca atttgtatca gaacaaacgg aaaaattaaa
     2401 agatactatc tctgatactg taaatagtga tgaggcagct aaataagcaa tcgtctagga
     2461 agtattattg actagtggtg ataagtttga gctggtattt accagctctt tttgtcacgg
     2521 ttaaaaagac acggactatt gtcatctctc taaaaatatg atttaattag atagaaattt
     2581 ttcaaattgt tagaaaggag taaattaagt gataagtcct aaaaaagaga taacaagttc
     2641 aaaatatcaa aaaattgcta tttcagtagc tcagcgtatc gcaaatggcg aatatgaagt
     2701 gggcgaaaag ttaaagtcaa gaactactat cgcctcgaca tttaatgtat caccagaaac
     2761 ggctcgtaaa ggcctgaata ttttagctga cttgaaaatt ttgactttaa aacatggtag
     2821 tggagctatt gtgctgtcaa aagaaagagc tattgagttt ataaatcaat acgaatcaac
     2881 ccattccatc gctgttttaa aagaaaaaat acgtgagact attaatgatc agggaaaagc
     2941 aatggaaaaa atggctgttt tggtcaatga ttttttaatg caaagtcaat ctgttagtaa
     3001 gcaataccca ttagcacctt atgagattat ttgtagtcaa gattcggaac attttggcaa
     3061 atctattggt gtgttaaata tctggcacca gacaggagca actatcgtcg ctattgaaca
     3121 tgctggtcag tttatcgttt caccaggtcc ttattctgtt attgaaaaag gcgatcatat
     3181 ttatttcgtt ggtgatgagt cagtcatttc acgaatgaag acctttttta atttacgaaa
     3241 aggattataa gtttgagggc ccacctcaaa ctttttttgt tgctgttgag cattattaaa
     3301 agaggcagat catcatggtt tgtctttcca aaatatctgt ttagacagtt gttttggatt
     3361 gggagcatga tttcagactc ttttgatgtt ttttaattgt ctagagtaac tgctttaaga
     3421 ataatatcgg caacctcttc agtggtttct tgatagccat tagcaatcca atagctgata
     3481 atgctctcaa tactggcaag gtaaacttct aaagcatagg gataaggaat atggtattga
     3541 ttggtcacaa tgtcttggaa accagtaata gtagtcaaaa attggtaaca gaaatccttg
     3601 atgagttgcg gaaatttgag gtaagaaata gtagcgagtg cggttataaa ttctctatgt
     3661 tctataaggt aggacaaggt ttgattgagg acttgtcgag tgtctgtgta gatttcagcc
     3721 tgatttaaca aacggtacaa atcatccaga gtgtcatttt tgaaatgatt catcatgtca
     3781 aatttatcag tgtagtggag gtagaaggtt cctctattga taccagcctt tttagtaagg
     3841 tcagagacag taagagtttc aaaagattgt tcagttagta aggtggttaa ggcggtcttt
     3901 acataagctt tggtttcagt atgtcttttg gtcattctca ggtgctcctt aaatcaacag
     3961 ttgcgtcaga tttgttcttg ttaaatactt atggccatta tataataagg ctataattaa
     4021 atcaacacta tgttcaaata atggaggggc agatggcttt tattgaatta aaacaggttt
     4081 caaaatctta tcaggtagga gagacgactg tttttgccaa ccacgaagtg tcttttgaga
     4141 ttaataaagg agaactagtg gttattttgg gagcatccgg agctggtaaa tcgacagtcc
     4201 tcaatatttt ggggggcatg gacacagtag atgcaggtca agtaattatt gatggcaaag
     4261 acattgctca ttacacgtct aaggccttaa ctcagtatcg tcggaatgct attggtttcg
     4321 tttttcaatt ttataatttg gttcctaatt tgacagctaa agaaaatgtt gaattagcag
     4381 ttgaaattgt agcagatgct ttagatcctg tgaccatttt aaaggaagta ggactcagtc
     4441 atcgtctgga tcattttcct gctcagctct caggtggtga acagcaacgg gtttcgatag
     4501 cacgtgcctt agctaaaaac cctaaattgc ttctttgtga tgaacctaca ggtgcccttg
     4561 actaccaaac aggaaagcaa attctaaccc tcttacagga tatggcacga actaagggga
     4621 ccacggtagt tattgtaacc cacaatgctg ctattgctcc gatagctgat cgtgtcattt
     4681 ttatgcatga tgcacaggtt accaagacag tcattaataa ggaacctgca agcattgaga
     4741 caattgatta ttaagaggaa ggtgtcgtat gataaagaaa accttatgga aggatatttt
     4801 gagagctatc aagaatagta aagggcgatt catttcctta tttttcctaa tggccttagg
     4861 ctcctttgct ttagttggac ttaaagtcac cggtccagat atggaacgaa cagccagccg
     4921 ttacctcgaa agacaccagg tgatggacct aacggtactg gcttctcatc aattttccca
     4981 agccgataaa caagagttag atacgttaaa aggggctcat ttagaatatg gtcatttact
     5041 tgatgtcagt ctaactagca accagaaatc tcttaggctc tatagcgtgc caaagaaagt
     5101 gtctaagcca gtcttggtta aagggagttg gtcaaagcga gagacagatt tggttttatc
     5161 ctcctcactt gctaaaaact atcagattgg agatgaacta gcagtcacct cacctatgga
     5221 aggtttgctg acgacaaccc attttcaagt ggttggtttt gcgaattctt cagaagtttg
     5281 gtccaagtcc aatttaggaa gttcctcaac cggagatgga agtctctacg cttatgcttt
     5341 tgttaatcca aacgttttta aaagtgcctt taacctgctg cgcatccgtt ttagtaatct
     5401 tcgtctgaca aacgcttttt ccaaagacta tcaaaaaagg gtaacccaaa accaagctca
     5461 tttggataat ctacttaaag ataatggcca gaagcgttat gatgacctcc aaaatcagta
     5521 tgaccttgcc ttaaaaaatg gcagagtagc acttgcaaag gaaacagtga aactagctgc
     5581 gagtgaggag aacttaactt ttttagaagg ctctgcttta caagaagcta agcatcagat
     5641 tgaacaaggc aaacaagcat tagccaagga ggaaaagcag ttagagcagg tgcaggctac
     5701 aaaagataag ctagaaaaac ccagttacct gacttataat cgctcgaccc taccaggagg
     5761 agaaggatat catacttatg caacttcaac gacctccatt tcaaatgttg gaaatatttt
     5821 tcctgttgtt ctttatctcg tagctgctct agtagccttt accaccatga cacgttatgt
     5881 tgatgaagaa agaacaagct ctggtctatt aaaagccatt ggttattcta acaaggatat
     5941 cagtttaaag tttcttatct atgggctttt agccagtttt ttagggacaa ctttaggtat
     6001 tattggggga acttacctct tatctgcctt gatttcagag atcttaacag gagctttgac
     6061 tattggaaag actcaccttt atagttattg gttttataat ggcatagctt acttgctggc
     6121 tatgttatct gctgttttac ccgcctactt aattgtcaaa aaggaattat tcctcaatgc
     6181 agctcagtta ttgctgccca aacctcctag taagggggca aaaatctggt tggaacacct
     6241 tacttttgtc tggaaagccc tgtcctttac tcacaaggtg accatacgta atatttttcg
     6301 ctataaacaa agaatgctga tgaccattgt aggcgttgca ggctcagtag cccttttatt
     6361 tgcaggctta gggattcagt cgtcattagc caaagtagtt gagcatcaat ttggtgattt
     6421 aacgacttat gatattttgg ctgtcggttc ggccaaagcg acagcgacag agcaaactga
     6481 cttagctagc tatcttaaac aagaacctat tacagggtac caaaaggtat cttatgccag
     6541 cttaaccctt cctgtaaagg gattacctga taagcaaagt atttccattt tatcaagttc
     6601 agctacttct cttagcccct attttaatct gctggatagt caggagcaaa agaaggttcc
     6661 cattccaacc tctggtgttt tgatttctga gaaattagcc tcctattaca aggtaaaacc
     6721 aggtgatcag ttggtattga ctgatcggaa aggacagtct tataaagtga cgattaaaca
     6781 ggttattgac atgacagttg gccattacct gataatgtct aatacctatt ttaagaatca
     6841 ttttaaagga ttggaggctg ctcctgccta tctgattaag gtaaaagaca aagatagcaa
     6901 gcacataaag gagacagcca gtgacttgtt aaccttaaaa gcgattagag cagtttcaca
     6961 aaacgccaat catattaaat ctgttcagct agtagtcacc tctcttaatc aggtcatgac
     7021 cctccttgtc ttcttgtcta ttttattagc aatcgttatc ctttataact taacgactat
     7081 taatattgct gagcgtattc gagaattatc cactattaaa gttctgggat tttacgatca
     7141 ggaggtcacc ttatatattt atcgagaaac tatttcgcta tccctagtag gcattctttt
     7201 aggtatctat ttaggaaaag gcctgcatac ttatatcatg acaatgattt caactgggga
     7261 tattcaattt ggtgtaaagg ttgatgctta tgtttatcta gtgccaattc tagtaatcct
     7321 tagcttgtta gcggtattag gtatctgggt taatcgccat ttaaaaaagg ttgatatgtt
     7381 agaagctttg aaatccatag attgattggt gaaaagacag atatagacag cacttgaaaa
     7441 gttaatcata ttatgatagt atctaaatac tataatttaa tagaaagata ctatgtatgg
     7501 aaaatagcat tgaggcctat ctagcagata gcagagaaaa ggtaacatta ggtacagttg
     7561 ttgattgttt taaaggaaaa gcagtctcaa gtaaggttgt gccaggtgat gtgggattgg
     7621 ttaatttatc tgacatgggc accttaggga ttcaatatca ccaattaaga actttccaga
     7681 tggatcgtag gcagctcttg cgttaccttt tggaagacgg agatgtctta atcgcctcta
     7741 agggaaccct aaaaaaagtc tgtgtttttc ataagcaaaa tcgagatgtg gtggcatcat
     7801 caaatatcac tgtcttacgc cctcaaaaac tgttaagggg ctactatatc aagttctttt
     7861 tggactcacc tattggtcaa gcgctcttgg acgcagcgga ccatggcaaa gatgtgatca
     7921 atctctcaac gaaagaattg ttggatattc caattcccgt tattccttta gtgaaacagg
     7981 actatttgat taatcactac ttgagaggat taacagatta tcatcgtaaa ctcaatcgag
     8041 cagaacagga gtgggaatat attcagaatg aaattcaaaa aggattaggt taggagtctt
     8101 gtcatgggtt cagttgaacg tacattaaaa atgaccttgg ctaccattgt tgccatttta
     8161 attgcttacc aacttcatct ggattatgca atgtcagcgg ggatcattgc cctgttaagt
     8221 gttttagaca cccgtaagtc cagtttggtg gtcgctagga atcgcttgct atcttttttc
     8281 ctggcttttg ggattgcgat gatgtgcttt agcttgtttg gttttacgac agtaggtttt
     8341 atgtgttatt tgttaattat tattcctttg ctctatcact ttcagataga ggcaggactg
     8401 gttcctataa cagtacttgt gacacatttg attgctaaaa agagtattgc attacctatt
     8461 ttatcgaatg agtttatgct cttttttgta gggactagtg tcgctttact atttaatgct
     8521 tatatgggtc cacaagatca gcaaattcgg tactatcacc aaaaagttga atcggatctg
     8581 aaaggtatct tataccgttt tgaaagtttt ctactggaag gaaaaggtca aaatgaaggg
     8641 ctcttgatca aaaaccttga taaaatatta gatgaagctc taaaactagt ttatcgagaa
     8701 aggcataatc aactatttca gcagaccaat tatcaagttc attattttga gatgcgacga
     8761 caacaaaata gactattggg acaaatggct atcaacgtaa acacgttaat gagacaaagt
     8821 aaggaaagta ttcttttgtc acatcttttt cacgaaactg cttgtcagct aagcgaacaa
     8881 aatccggctt taaccttgat tgatgatatc gaacaattgc ttgaaacctt tcgtcatggt
     8941 gatcttcctc aaactcggga ggaatttgag cgacgtgcgg ttttatttca gctcttacaa
     9001 gacttggaac gttttatctt attaaaggta gagttttatc aggattatca aaatgactaa
     9061 atctctattg cacacttctt atctatgaca gcttgggact taacttcctg agcttttttg
     9121 ctataataaa aacaagaatc tcagaatgga gacaagatga acgaaataaa atgccctcat
     9181 tgtcataccc tatttactat caatgagtca gagtatagtc aattattgga acaagtcaga
     9241 gggcaagcct ttgatgaaga attgcaaaaa cggttaatca atgagatggc cttgctggaa
     9301 gaaaaagcga agcatcaatt gcatgaggtt gtcgcaaaaa aagaaacagc tattaccagc
     9361 ttgacaaacc aattagagca aattgaaaaa gaacaagcct acctcagaca agaagaactg
     9421 gctaaaaaag accagcttat tgcttcttta gaagcgaaac ttgataaatt agcctcacaa
     9481 aatgctttgg aactggccaa tcaattagct gagaaggaca aagaagtggt tagtttgacc
     9541 aatcagctgg acaaattagc acttgaaaaa gatgccacgt tccagtcaaa gcttgctaca
     9601 atcgaaaaag aacgcgatgg aatcaaaaac caattggcgc ttcaagctaa agaaagtgaa
     9661 ttgtcgttgg cttcggtgcg cagcgactac gaagcacagt taaaagcggc taacgagcga
     9721 gttgaatttt ataaaaattt caaagcgcag caatctacca aggctatcgg agagagtcta
     9781 gaactctatg ctgaaacaga gtttaacaaa gtaagaagct atgcttttcc caatgctagc
     9841 tttgtcaaag ataaccaatt gtctagtcgt gggtcaaaag gggactatat ttaccgagag
     9901 gttgatgcca atggtgtaga aatcctctct atcatgtttg aaatgaagaa cgaggcagac
     9961 accaccaaaa caaaacataa aaatagtgat tttttcaagg agttagataa agaccgtcgc
    10021 gaaaaagatt gtgagtatgc tgttttggta agcatgttag aagctgataa tgattactac
    10081 aatacaggga ttgttgatgt cagtcatgag tatcaaaaga tgtatgtggt tcgtcctcaa
    10141 ctctttattc agttgatagg gattctgcga aatgcagccc tgaatagttt acattataag
    10201 caagagttgg ccttgattaa ggaacaaaat attgacatca ctcacttcga agaggatttg
    10261 gatcaattta agaatgcctt tgctaagaac tatcagtctg ccagcaacaa ctttaaaaag
    10321 gctattgacg agattgataa atcgattaaa cgcatggagg aagtgagacg ctttttaacc
    10381 actagtgaaa atcagttacg cttagctaat aataaattag aggatgtttc agttaaaaaa
    10441 ctgacccgcc aaaacccaac catgcgagag aaatttgcag ccctcaagga ccagtaagcc
    10501 tgaaagtgag tctttccctg ctaaaattgg ttaagcgttt attttggcaa aactgatgat
    10561 aaagcggtag aatagaatca gtaacaatta ctaattagag agaaaaatag acatgataaa
    10621 tggaatcatt aatttaaaaa aagaagccgg catgacctct catgacgcag tttttaaatt
    10681 acggaagtta ttacaagaaa aaaagattgg acatgggggg accttagatc ctgatgtggt
    10741 cggtgttcta ccaattgcag ttggtaaggc tactcgtgtg atcgaataca tgacagaagc
    10801 tggtaaagtt tatgaaggac aagtgactct cggttactca acaacgactg aggatgctag
    10861 tggagaggta gttgcgagat cttctttgcc agctgtgcta acagaagaac tcgttgatca
    10921 aacgatgacg acttttcttg ggaaaatcac acagacccca cctatgtatt cagccgtaaa
    10981 ggttaatggc cgtaaattat acgagtatgc gcgtactggt gaaagtgtgg aacgtcctag
    11041 aagagaagtg accatttctc tgtttgaacg caccagccct ttgaatttta ctgaagacgg
    11101 tctttgccga ttctctttta aagttgcttg tagcaagggg acttatgtgc ggaccttagc
    11161 agttgattta ggtcgagctc tatgggtaga gagtcatatg tccttcttgc agcgatcagc
    11221 ttcagcagga ttaaccctcg agactgccta tactttggga gaaattgctg atatggtttc
    11281 taaacaagag atgtcttttt tgttaccgat cgagtatggc gttgctgatc ttcctaaaat
    11341 ggttattgat gatactgaat tgactgaaat ttcatttggt cgccgtctta gtctccctag
    11401 tcaagagcca ctgttagctg cttttcatgg cgaaaaagta attgctattt tagaaaaaag
    11461 agatcaagaa tacaaaccca aaaaagttct tatttaaata gtttgagtaa gtagaatgga
    11521 taattatgga aattgaatat atcaaagatt atcgtgatat taaccaagaa gatgatacag
    11581 tcttggtatt gggatatttt gatggattac accgtggtca taaagcctta tttgaaaaag
    11641 caagagaggt tgcaaataaa gaagggttaa aagtagtcgt ttttacgttt acagagtctc
    11701 cgaaattggc tttttcgcgt ttttctcctg agttattgct tcatattact tatccaaaga
    11761 agcgttatga aaaatttgcc gattacggtg taaataaatt atatttggtt gattttactt
    11821 ctaaattctc taaggtctca tctgatcatt ttataactca ttatattaaa aacctgaaag
    11881 caaagcatat tgttgtcggt tttgactata agtttgggca taattgtaca gatagtgatt
    11941 atttaaccag aaattttgaa ggacaagttt ataccattaa ggaaataaaa gaagatcatc
    12001 gaaaaatttc tgctacatgg ataaggaagc ttattcaaga aggtaatgta gttaaagcaa
    12061 accacttgtt aggctatgac ttatcgacaa gggggagagt cgttcacggg gatgcgcgtg
    12121 gccgtactat tggtttccca acagccaatc tagctccaat tgacaacaca tacttaccag
    12181 ctgatggagt ctatgttaca aatgttattg ttgctaataa aatctatcgc tctatgacga
    12241 gtttaggtaa aaatgtgaca tttgggggga aagagttgcg tctagaagtt aacatttttg
    12301 actttgacga agagatctat ggagaaatta ttgagattgt ttggttagat aagattcgag
    12361 acatggaaaa attcgaggga atcgaagatt tgactgatag attggaatat gataaacgaa
    12421 cagcattaaa ttggaaaaaa gatagcaaat tatcttaaat ttttgtataa taaggtaagt
    12481 aaatttgata gaggaaaaat atggttacct tatttttatc acctagctgt accagttgtc
    12541 gtaaagcaag ggcatggtta gtaaaacacg aagttgactt tcaagagcat aatattatta
    12601 caagtccatt aagccgtgat gaattaatgt caatcctctc ttttacagag aatggaacgg
    12661 aggatattat ttcaacacgt tcaaaggttt ttcaaaaact tgatgttgat gtagaagagc
    12721 tttcaatttc agatttaatt gatttaattg ctaaaaatcc aagtcttctt cgtcgcccta
    12781 ttatcatgga tcaaaaacgc atgcaaattg gttttaatga agatgaaatt agagcgtttt
    12841 tatcgagaga ttatcgcaag caagaactac gtcaagcaac gataaaagca gaaattgagg
    12901 ggtgatatgt caggtaatta ttattatcca cttgacttaa gttggagcac tgaagaaata
    12961 tcctcagtgc ttcatttttt gaataaagtg gagttagcat atgaaaaaaa agtagatgca
    13021 aaacaactcc tagatagtta taagacttat aaaaccattg tcaaaagtaa agctcaggaa
    13081 aagcaaatcg accgtgattt tcaaaaagtt agtggttatt cgacctatca agttgttaaa
    13141 aaagcgaagg caattgagaa aggattcttt tcccttggaa actaaatacg catttgcaag
    13201 gcaaatcatt aaagaagcag ggctttttat caagtcaaaa atgtcagagc agctagatat
    13261 tcaggtgaaa acacaatttg atgatctggt taccaatgtt gatcaagaaa ctcaacagtt
    13321 actgatggat cgtattcatc agacctatcc ttgtgatgcc attttagctg aagaaaatga
    13381 cgttcgtcac cccattaatc aaggaaatgt ttgggtcatc gatcccattg atggaacggt
    13441 taactttatt gtgcagggat cacagtttgc agtgatgatt gcctattatg aacaaggaat
    13501 tggccaattt gggcttattt atgatgtgat ggcagatcaa ttactagctg gtggtggtga
    13561 ttttgaagtt actttaaacg gtgataagct tccagcttac caagaaaaac ctttagagcg
    13621 tagcttaatt ggctgcaatg caggcatgtt tgccagaaat gatcgtaacc ttgctcatct
    13681 aattgcaaag actttaggtg taagggttta tggtggtgca ggtatctgca tggttaaggt
    13741 catgaagcag gaattactgg cctatttttc atttatccaa ccatgggatt acgcagcagc
    13801 aaaagtacta ggcgataaac tgggttatgt tttactaact attgacggat atgagcctga
    13861 ttttcaaaca cgtcaaaaaa tcatgtttgt tcctaaatgc caactcacta ggatagcgtc
    13921 ttttctaaca aaggactctg ccgtcacact tgaagatcaa tagaaatgat gtcattacca
    13981 aaagaattta ttaacactta tcaagcaata ctaggcaaag aattagaaga tttcttggca
    14041 agttttaacc aagagccggt caatgctttt cggattaacc ccttaaagaa tcaactaaaa
    14101 acctttgaac accccattcc aaatacctta tggggatatt atggtaaact atctggcaag
    14161 tctccagagc atgtgtcggg gttggtttat tcccaagaac cagcggctca aatggtggca
    14221 caagtagcgg ctcctcaaaa ggggagtcgt gttttagact tggcagctgc accaggagga
    14281 aaatcaactc atttattagc ttatttagac aatacaggtc ttttagtcag caatgaaatt
    14341 tctaagaagc gcagcaaggt tttagttgaa aatatcgaac gttttggcgc tagaaatgtt
    14401 gtggtgacta atgaatccgc agatcgttta gccaaggtat tttctcatta ttttgatact
    14461 atcgtctttg atggaccatg ttctggtgaa ggaatgtttc gcaaggaccc tgatgctatt
    14521 caatattggc atcacgggta tcccgcagaa tgcgccaagt tgcaaaaatc tattttagaa
    14581 gacgccttaa ccatgttaaa accaggagga gaattgatat actccacctg tacttgggct
    14641 cctgaagaaa atgaagatgt tgtgcagtgg ctcttagaga cttatacctt tttagaattg
    14701 gtcgatgttc caaaactaaa tggaatggtt agtggtatcg gacttccaga gactgctaga
    14761 atgtaccctc atcgttatca aggggaaggg caatttgtgg ccaagctaaa agataagcgt
    14821 caagaaggac aaagcactaa actaaaagca cctaaatcca acttgaccaa agaccagtta
    14881 cgtctttgga aaatgtttga aaaagaccat ctaaacctta ccctatcagg aacactacaa
    14941 acctttggtg attacctata cctcttgcca gatggtcttc ctgctttaga tggccttaaa
    15001 attgctcgga acggtttaga attaggcgtc ttcaagaaga aacgctttga accctcttac
    15061 gccttgggat tagctttaaa gcctgatgag gtccgatcat ctattgagct tagagaggaa
    15121 gactttagac cttatgtgtc aggtaacgtc attactttgc cccaatctta tccaacaggc
    15181 tggtaccaat tactcattaa tggcaatggt ctaggatttg ccaaggtcgt tgacaagacc
    15241 atcaaaaata atttccctaa agggttaaga ttttagcgta aaaaataata ttaaaaagtt
    15301 aaatatttaa agaaaaaatg ctttcaaagc ataggtagat atgttactat aaagaatatg
    15361 tggcacttag tttctacata tatacccttt atttttacga ataggaaata aataatgaaa
    15421 atgaaaaaaa aattcttttt gttaagtctt ttggtcctat caactttctt tttatccgca
    15481 tgttctagct ggattgataa aggtgagtca ataaccgctg taggatcaac agcactacaa
    15541 cccttagtag aagcagtagc tgatgaattt ggaagcagta atctaggcaa gactgtcaat
    15601 gttcaaggtg gtggttcagg tacagggttg tctcaagttc aatcaggagc tgtccaaatt
    15661 ggaaatagtg atgtctttgc ggaagaaaaa gatggtattg atgcttctaa attagttgat
    15721 catcaagtag ctgttgcagg acttgcagtt attgccaatc ctaaagtcaa ggtttccaat
    15781 ctcagtagtc agcagttgca aaagattttt tcaggagaat ataccaattg gaaacaagtt
    15841 ggaggagaag atcttgcgat ttcagtgatc aaccgagcag caagttctgg ctcacgagca
    15901 acctttgaca gtgttatcat gaaaggggtc aacgctaaac aaagtcaaga gcaagactcc
    15961 aatgggatgg ttaaatcgat tgtttcacaa acaccaggtg ccatttctta cctttccttt
    16021 gcctacgttg attcatctgt taaatctttg caattaaatg ggtttaaggc aaatgctaag
    16081 aacgtggcta caaatgattg gccaatctgg tcctacgaac acatgtatac caaagataaa
    16141 ccaacagggt tgaccaagga atttcttgat tatatgtttt cagatgaagt acaacagaac
    16201 attgttacac atatgggata tatttcgata aatgatatgg aagtggtcaa atctcatgat
    16261 ggaaaagtaa caaaaaggta atggaggaat tatggaaaat caggaattgg ctaaaaaatt
    16321 agcctcacca tcaaagaatt caagacttga aacctttggc cgcaccatta cgtttttgtg
    16381 tctggctttg attgttttta tcgttgcgat gattctgatc ttcgttgctc aaaaagggtt
    16441 gtcaaccttc tttgttgaca aggttaatct ttttgatttc ttgtttggaa aggagtggca
    16501 accaagtgtg aaaaatgcgg ccggcattcc ttatcttggt gctctgccaa tgattacagg
    16561 atcctttttg gttaccattt tatctgctat tattgccacc ccatttgcca ttggcgcagc
    16621 cgtttttatg actgaaatct cacctaaata tggcgctaaa ttattacagc ctgcggttga
    16681 gcttttggtt ggaattcctt cggttgttta tgggtttatc ggtttgcaag tgattgttcc
    16741 ttttatgcgc tctatctttg gtggcacagg ttttggaatc ctatctgggg tctgtgtctt
    16801 gtttgttatg attttaccaa cagtgacttt tatgacaaca gacagtctgc gggcggtgcc
    16861 tcgtcattac cgcgaagcgt ctatggctat gggagcaacc tgttggcaaa ccatttggcg
    16921 tgttgttctt aatgcagctc gtccaggtat ttttactgcc attattttcg ggatggcgag
    16981 agcttttggt gaagccttag ccattcaaat ggttgtcggt aactctgctg taatgccaag
    17041 ttcactaacg acaccagccg caaccttaac gtctgtcttg acaatgggta ttggtaacac
    17101 ggttatggga acggtccaaa ataatgtgct ttggtccctt gccctagtct tattattaat
    17161 gagtctggcc ttcaattccc ttgtcaaatt aatcacgaaa gaaagaaaga gaaattatga
    17221 acgctaaaaa agtcgataaa gtagcaactg gtactcttta taccattgct ggaattattg
    17281 tagctatttt agcttcctta attctatata tccttgtccg tggcttgcca cacatcagct
    17341 ggtccttttt aacaggaaaa tcgtcttcgt acgaagctgg tgggggaatt ggtatccagt
    17401 tgtataattc cttcttccta ttgattgtta ccctgattat ttccattcct ttatcaactg
    17461 gagcggggat ttacttggct gaatatgcca aaaaagggcc tgttaccaac tttattagaa
    17521 cctgtattga gattctgtct tccctaccat ctgtggttgt agggctcttt ggttacttga
    17581 ttttcgttgt gcagtttgaa tatggctttt ctattatttc agggtctctt gctttgacgg
    17641 tctttaatct tcctcaaatg acccgtaatg ttgaagatag tttactgcat gttcatcata
    17701 cccaaagaga agcaggatta gctttaggcc tgtcacgctg ggaaacagtt ttttatgtgg
    17761 ttatcccaga agctctcccg gggatggtaa caggtattgt cttagcttca ggtcgtattt
    17821 ttggtgaagc ggcagcgctt atctatactg ctggtcaatc agcaccagct ctggattggt
    17881 caaattggaa tccacttagt gttactagtc caatctctat tttccgtcaa tctgagaccc
    17941 ttgctgttca tatttggaaa gtcaatagcg aagggacaat tccagatgct actcttgtat
    18001 ccgcaggaag tgcagcagtg ctattaattt ttatccttat tttcaacttt tcagctcgct
    18061 ttattgggaa gaaacttcat tctaaaatga cagcagcgaa ataagatatt ggagaaatca
    18121 tgacagaata taattggaat gaacgtcata tcatcacttt tcctgaagaa acgcttgctc
    18181 tagctaccaa ggatttacat gtttactatg gtgcgaaaga agctatcaaa gggattgata
    18241 tgcagtttga aaaacataaa ataacagcct tgattggtcc atctggttgt ggcaaatcaa
    18301 cttatcttcg tagtcttaac cgcatgaacg ataccattga tattgctcgg gttacaggtg
    18361 agattcttta tcagggcatt gatgttaatc gaaaagacat gaatgtttac gagattcgca
    18421 agcacttagg aatggttttt caaagaccca atcctttcgc taaatccatt tataaaaaca
    18481 ttacctttgc ccatgaaaga gctggtgtga aagacaaaaa agttttagat gaaattgttg
    18541 agacttcact gaaacaagcc gcgctttggg atcaagttaa agatgacctt cacaaatcag
    18601 ccttcacctt atcagggggt cagcaacaac gcttgtgtat tgctagagcc atttctgtca
    18661 aacctgatat tctattaatg gatgaaccag cctctgccct tgacccgatt gcaacgatgc
    18721 agctggaaga aaccatgttt gaattgaaga agaattacac gattattatt gtgacccaca
    18781 atatgcaaca agcagctcgt gccagtgatt acactgcctt cttctattta ggcaacctca
    18841 ttgaatatga taagacacgt aatattttcc aaaatgcgca atgtcagtca acaaacgact
    18901 acgtttcagg gcactttggt tagaaaggta atatgatgac agaacctatt ttacagatca
    18961 gagacctttc tgtttactac aatcaaaaga aaacgttaaa agatgtctct ttggaccttt
    19021 atccaaatga aataacagct ttaattggtc cttctggatc tgggaaatca acgctcttgc
    19081 gttctatcaa ccgtatgaat gatttgaatc ctgaagtaac cattacaggc tctatcgtct
    19141 ataacggaca caatatttat agtccaagga cagatacggt tgatctacgc aaagaaattg
    19201 gtatggtatt ccaacaaccc aacccatttc cgatgtctat ttatgagaac gtggtttacg
    19261 gccttcgctt aaaaggtatc agagataaat caatcttaga ccatgctgtt gaaagctctc
    19321 ttaaaggggc ttccatttgg aatgaagtca aagatcgttt acatgattct gcggttggct
    19381 tatcaggcgg acagcaacag agagtttgca tagctagagt tcttgctacc agccccagaa
    19441 taatcctttt agatgaacct acctcagctc ttgatcctat ctcagctggg aaaattgaag
    19501 aaacattatt gttattaaaa aaagattata cactggctat tgtgacccgc tcaatgcagc
    19561 aagcctcacg tttatcagat cgaacaggtt ttttcttgga gggtgactta cttgagtgtg
    19621 gtcctacaaa agctatgttt atgaatccta agcgcaaaga aaccgaagac tatatttctg
    19681 gtaaatttgg ttgagaaaga cgtatatggt tgacccctct gtgtttttat tataatgact
    19741 agattgtagg aagaaaaaat catgttaaga acgaaatttg aagaagaatt agataaattg
    19801 cataaccagt tttattcaat gggaacggaa gtattggcac aaatcaataa aactgttcgt
    19861 gcctttgtta gtcatgatcg tgaattggcc aaagaagtta tcgaagaaga tgacactatt
    19921 aataattttg agacaaagct tgaaaagaaa tcgctggaaa ttattgccct acaacagcca
    19981 gtttcgaatg atcttagaat ggttattact gttttaaagg catcaagtga tattgaacgt
    20041 atgggtgacc atgctgcttc gattgccaag gccactattc gtatgaaagg tgaggagcgt
    20101 attccagtgg ttgaagaaca aatcaacctt atgggaaaag ctgtgaaaca gatggtagaa
    20161 gaagccctta atgcatatat caatgctgac gatacaaaag cttatgaaat cgcagcttct
    20221 gatgagatta tcgatcaata tttccgtaat atccaaacct tagctgttga agaaatccgt
    20281 aagtctccag atgctgtttt tgcaggaaaa gaatattttc aagtattaat gtatttggaa
    20341 cgtattggtg actatgctcg caacatttgt gaatggattg tttatcttaa aactggaaaa
    20401 attattgaat tataagaaat tatgctataa tgagactggg aaacagcttg ccagtccctg
    20461 gttttatacg aaattgtgca agttagaaaa tgaactaaca agaaaagttt tctttgctga
    20521 atctgatttt gttaggttaa caaatatatg tagacagaga tgatacttag gtatcatctg
    20581 ttgatctatt atgatttgtt attagaatta aggagttata tgaagacagt agaacatctc
    20641 atcgaaacat ttgtgcctga aaattataac atctttttag atattaatcg tcagactaaa
    20701 acatttacgg ggaatgttgc gattaatggt gaagctttag ataatcatgt ggctttccac
    20761 caaaaagatt tggacattaa gtctattttg ctcgataatg aagctgttat ttatcaagtg
    20821 gataacgaca atgaagttgt gcgtgttgaa ttacctgaaa caggaatgat gacgcttgtc
    20881 attgaatttt caggtagcat tacagataat atgacaggta tttacccttc atattacact
    20941 aaaaatggtg agaaaaaaga agtcatttca actcagtttg aaagccattt tgcacgtgaa
    21001 gctttcccat gtattgatga accacaagca aaagctactt ttgatttaag cttgaccttt
    21061 gatcaagaaa ttggtgagat agcgttatcg aatatgccag aagtcaatat tgaccgtcgc
    21121 gaggagactg gtctttggac atttgatacg acaccaagaa tgtcatctta cttgctagcg
    21181 tttgcgctag gagaacttca tggaaagact gtagaaagca aaaaaggaac aactgttggt
    21241 gtgtatgcga caacagcgca tcctcttagc tcgcttgatt tttcattgga tattgcagtt
    21301 cgtgttatca acttttatga agactatttt ggagtacatt acccaatccc gcaatctctt
    21361 aatattgcgc ttccagattt ttcatctggt gctatggaaa attggggttt aatcacttat
    21421 cgtgaaattt atttattagt agatgaaaat tcgactgttc aaagccgaca acaagttgca
    21481 cttgtaatcg cccacgagat agcacaccaa tggtttggaa acttggttac aatgaaatgg
    21541 tgggacgatc tgtggttaaa tgagagtttt gccaacatga tggaatatgt ctctatcgag
    21601 gctattgagc cgtcttggaa aataatagaa gatttccaaa caggtggtat tcctcttgct
    21661 ttaaaacgtg atgctactga tggtgttcaa tcagtgcatg ttgaagtcaa tcatccagat
    21721 gagataaata cattgtttga tcctgctatt gtctatgcaa aaggtagtcg tttgatgcat
    21781 atgttacgcc gttttattgg tgatcgtgat tttgccattg gcttacatca ctactttgaa
    21841 aaatatcaat atcgaaatac tgtcgggcgt gatttatgga atattttatc agatacttca
    21901 ggcaaagacg tagctgcttt tatggatgct tggcttgaac aaccaggtta tccagtttta
    21961 acagctcgcc ttgaaaacga tcaattgatt ttgagtcaaa aacaattttt tattggaaaa
    22021 ggtgaagaaa aaggtcgcct ttggccgatt cctttaaaca ctaattggca tggccttcct
    22081 gaaactttga cagaggcaga aatggtcatt ccaaacttta gtcaattggc agcagaaaat
    22141 gaaggagctc tacgtttcaa tattgataat acagctcatt acattacaga ctaccaagga
    22201 tcattattag atgccttagt cactgaactt gcccaacttg ataatacttc tgctttacaa
    22261 gttattcaag aacgtcgttt attggcagat agtggcttga tttcctatgc tgaattggtt
    22321 gacttaattg ctcaattgga cgattctaag tcttatatgg tagcagaagc ggttcagcaa
    22381 gtggtctctg gtttgaaacg cttcattgat gaaggaagct tagcagaaaa atcctttaat
    22441 cgcttggtaa ccaccatcta tcaagaagac tttaatcagc atggttttga gaaaaaagct
    22501 gacgaatcag atgaagatga gatggtacgt caggttgcct tgggccgctt gtggctagca
    22561 gaaaatcaga cgattattga cggattgaga actatttttg aagcatatca aaacaatatt
    22621 gcaagtattc cagcagctgt tcgtcgcttg gttttagcca accaaatgaa atattttgaa
    22681 acagatagct tggttgatat ttattttgag acttatgtag caactactga taataattta
    22741 cgcagtgatt taacggttgc ttttagtcaa acaaaacaac caactactat tagacgtatt
    22801 cttgttagtt taaaagataa agatattatt aaacctcagg atttatctta ttggtacaat
    22861 gctttgcttg gacaaagctt tactcaagat atcatttggg aatgggctag agaaaattgg
    22921 gattggatta aatctgcact tggtggagat atgagctttg ataaatttgt tatttatcca
    22981 gcaagtaact ttaaaacacc aaaacatcta gcagaatata aatctttctt tgaacctaag
    23041 ctagatgata tggctattag ccgtaatatt accatgggaa ttaatgaaat tgaagcacgt
    23101 gttgctttga tcactaaaga aaaagaagca gttattgcag ctctcagtca ttactagacc
    23161 ataagcaaaa gagttgggag gactttccag ctcttttttg tagtgagcta gcttcctttc
    23221 ttagttccgt ctataactaa atgattatcc atagtttaat tcaaaaggag attgcacttt
    23281 aaagcgaagg ctattagcct cttaatactt tttttaccag gtggtgaata agaacaatta
    23341 taaatgattt aatcattatg ctttaaatgc cttaaaggag tattaatatt ttagtaaacc
    23401 ttaagctttt tgtagtatag tctcgttaga aattgttatc agaaatgaga ctaagttaaa
    23461 tgaagtgata gttactttat ttaagaacaa ttgaggtaga gacatgataa aaatattatt
    23521 agtagaagat gacttgagct tatcaaattc catttttgat tttttagatg attttgcaga
    23581 cgtcatgcaa gtttttgatg gcgatgaagg tttatacgaa gcagaaagtg gtatttatga
    23641 tttgattttg ttagacctta tgttgccgga gaaaaatggc tttcaagtct taaaagaatt
    23701 gcgagaaaaa gatattaaaa ttcctgtatt gatcatgaca gctaaagagg gtttagatga
    23761 taaaggtcac ggatttgaat taggtgctga tgattatctg acaaagccgt tctatttaga
    23821 agagttaaaa atgcgaattc aagcactgtt aaaaagaaca gggaaatttg cagataaaaa
    23881 tattagtttt ggcaatttag tggtcgactt agctcgaaaa gaggtgaagg ttgaagggaa
    23941 agtagttgaa ttacttggca aagaatttga tttgttggtg tatcttttgc aaaatcaaaa
    24001 cgttatttta ccaaagacac aaatttttga tcgtttatgg ggattcgata gtgatactac
    24061 gatttcggtt gttgaagttt acatttctaa aataaggaaa aaattaaaag gtacccgttt
    24121 tgttaacagg cttcaaactt taagaagcgt agggtatatt ctaaaaaaca atgaataaat
    24181 taaaaaaaga gattttatca gataactata accacttttt tcattttttt gcggttttta
    24241 caggtatttt tgtcattatg actattatta tcttacagat tatgcggttt ggcgtttatt
    24301 cgtcagttga tagtagttta gtttctgtta gtaataatgc aagtagctat gctaatcgta
    24361 cgatggctag aatatcttct ttttactttg atactgaaaa taacattatt aaggcgctgc
    24421 ctgattcaga tagttctaag ttattaggaa cgcctgcagc taatacagat atcattttgt
    24481 ttagtgctaa tggaacaatt ttaaatgctt ttgatgcgtt ttctaactat caaaattttc
    24541 atttagataa acgccggttg gggagtattg aaaccaccag tttaatgaat ttttatggac
    24601 aagaagaaaa ataccatacg ataactgtaa gggttcatat caaaaattat cctgcagttg
    24661 cctatatgat ggcagtagta aatgtggaac aattagaccg cgctaatgaa cgttacgagc
    24721 gcattattat tatagttatg agtgtttttt ggctaatttc tattttagca agtatttatt
    24781 tagccaagtg gagcagaaaa cctattttag aaagctatga aaaacaaaaa atgtttgttg
    24841 aaaatgctag tcatgaacta aggacccctt tggcggtctt acagaatcgt ctggaatcgc
    24901 tttttcgtaa gcccaacgaa acgatattag aaaatagtga gcatctcgct tctagtttag
    24961 acgaggttcg caacatgcgc atcttaacaa ctaatttatt aaatttagca agacgagatg
    25021 atggcattaa tccacagtgg actcatttag atacagattt ttttaatgct atttttgaga
    25081 attatgaact agttgctaaa gaatatggaa aaatatttta ttttcagaac caagtcaata
    25141 gatcgttaag aatggataag gctttactaa aacaattaat aacgatttta tttgacaatg
    25201 ctattaaata tacagataaa aatggtatta ttgaaattat agtgaaaaca acggacaaaa
    25261 atttattaat ttctgttatt gataatggtc cagggataac agatgaagaa aagaaaaaga
    25321 tttttgatcg tttttatcga gttgacaaag ctagaacacg gcaaacaggt ggatttggct
    25381 tggggttggc tttagctcag caaatcgtga tgtctttaaa aggaaatatt acagtaaagg
    25441 ataatgatcc taaaggtagt atttttgaag tcaaattata agctaaaaag ggcgaccaat
    25501 aaggtcagcc cttttaaagt tatttttaac tgtcaacgct ttattgtatt ttttgagttt
    25561 ttaccgtata aaaaaactcc agctggagtt ttcattggat atttattagc caagttttgc
    25621 agcaaggcgt gctttatcgc ggcttgcttt gtttttgtga atcaaacctt ttgattcagc
    25681 tttatcgatg cttgaagaag cagcgcggaa aagctcttca gatgggttag cttcaaatgc
    25741 tttgatagca gtacgcatag ctgatttttg tgctgagttt ttttcgtttg ctttaacgtt
    25801 aagttcagcg cgtttaatag ctgatttaat atttgccaat gttttcacct ccaaattaat
    25861 ctaactacat tattatatag aaaatatatg cgtttggcaa ccctttatca ctttttgagg
    25921 taaatttcat caattttatg atcagcactt ttatgtaaaa ttaattcagc acggttacgt
    25981 gtaggttcaa tgaatttttc aagattttct aaattaacgg ttttccaaac gttgcgcgca
    26041 aatgctatag cctcagatcg aggtaattgt gcatattggg cataataatt atgagggtca
    26101 cgttttgcta attttaaaat gcttagaaac cgttcaatgt accaggtttc gatgtgactg
    26161 ctgtctgcat caatataaat agaaaaatca aagtaatcac tcatatataa acgattgttt
    26221 tgttgatttt gaaaaacatt aataccttcg acgataagga agtcagggtt attaaatgat
    26281 tgtttttgat ttgggataat atcgtaaata tcatgagaat agacgggtgc aaaagccgtt
    26341 tgaccatttt tgatggaatc tagaaaatct aagagaagtt ccatgttata agattctgga
    26401 aatcctttac gatttaacag tccttgttca ataagaaact gattaggata taaaaaacca
    26461 tcagtcgtta ccagttcgac ttgactattg gggtgcgttc gtgataataa aagttgtaaa
    26521 agacgactag ttgttgattt accaacagct actgaaccag aaattccaat aataaaaggc
    26581 cttaattgaa tgtctttttt tagaaaaagg cttttagaaa aagaaagatt ttcttgcgca
    26641 attttgtaaa cctggatcag attaattaat ggaaggtaga tatcaatgac atcgttaata
    26701 ctgatgttat catttaagct agtaatactt ttaagttcct cttgtgtgag aagagctttt
    26761 gctttttgat gtaatgtttt ccaagactct ctagaaattt tttcaaagtt gataaattca
    26821 tttgacatgg tagctcctaa tcatttgaca gtgcttacat tataccattt tagttaaaaa
    26881 ataaataatt tttcgttagt atttacacga aaattaatac gattaactca aaaaagccac
    26941 acattcctag tacaattatc ctgctatatt ctaacaaata gttaaagttc ggtttttatt
    27001 tgtaatatag ataaaattgt taaaaaacaa gcgtcttgta aaccatttca aagtatgata
    27061 gaatgaaaaa gtaccttaaa aagataggaa aaatgatgac aaagatgtat tatgatgaaa
    27121 atccagatag tctacacgat attcatgaag taaaagtaga gctgcttaat catcccttta
    27181 cttttttgac ggattcaggt gtcttttcaa agaaaatggt tgatttcggt agtcaagtat
    27241 tattagagac actaaatttc aaggagaacg aacgtgtact ggacttaggt tgcggctatg
    27301 gtcctcttgg gattagtcta gccaaggttc aaagggtaga cgcaacctta gtggacatca
    27361 ataatcgtgc tcttgattta gctagaaaaa atgcaactaa taaccaagtg gcagtaacta
    27421 tttttcaatc aaatatttat gaaaatatta gtggccattt tgagcatatt attagtaatc
    27481 ctcctattag agcaggaaag agagtagttc atagtattat tgaaaaaagc atagattttt
    27541 tagtggttaa tggtgactta acgattgtga ttcaaaaaaa acaaggagct cctagcgcta
    27601 aagcaaaaat ggcaactatt tttggtaatg ttgagatatt aaggaaagat aaaggttatt
    27661 atgtattaag aagtataaag caaacctaat ttaaataatc aacaattgct catttaaatt
    27721 aggtttgctt aaatagtaaa gagttatttt taaattatag ataactaata ttatactagg
    27781 gtagcccctc tactcgagta acctttttta atgtatttta actgaaaaag gctttcataa
    27841 ttttaaaaaa atgttaaaat aggtgtataa caactgatta ccaagagatt attatcatta
    27901 tcttctaagg tgtcattgtt atggtgacaa tcaaccatac gctactaaag ataggtagcg
    27961 ttcgatcaca ggttgatcaa gtgattgata cagatattta taattttaca aatatctgta
    28021 tcaatccatg ttgaatagtc tatgcttcta aggtgtcgac gttaatgtac tgacgggacc
    28081 gttatagtct ttctttttgg agaaacgtcg gatgccagtg aactagttga agttaaagat
    28141 gttaattcaa acagtgctga agagattgat atcgttttga acggtcgttt acttcaaagc
    28201 aaagaaatta ccagtctatc ggagatgata taagtgctaa agttagatca cgttaattat
    28261 ctatcaagtc gggtgcatat ttcgttaaag tatctacagg ggtttcaata gatgtagcta
    28321 ttatgaaagg tgatgttaaa tggggactac tgatttagta agttgtgctg tacaagctag
    28381 tgagtatgct tatgtccctt actctcattt tccagtagga gcagctttaa aaacaaaaga
    28441 tggaacgatc tatactggct gtaatattga aaatgttagt tttggtctga caaattgtgg
    28501 agagcgaaca gctatattta aagccatatc tgatggtcat aaagaattgg ttgaaattgc
    28561 tatttatgga gaaactatgc aacctgtatc tccttgtggg gcttgtcgtc aggtaatggc
    28621 agaatttttt gctccttctt ctttagtcac tttaattgct aaaaatggtc aaactgttga
    28681 aatgactgtt ggagatttac tcccctattc ttttacagat ttaatataat ctgctttaat
    28741 ttctgttatc tagccgtctt ggctttatat ttactttcag caacttattt gctagaaatc
    28801 ttttttagga gggttcatat tcatgaacaa gaaatttatt ggtcttggtt tagcgtcagt
    28861 ggctgtgctg agtttagctg cttgtggtaa tcgtggtgct tctaaaggtg gggcatcagg
    28921 aaaaactgat ttaaaagttg caatggttac cgatactggt ggtgtagatg acaaatcatt
    28981 caatcaatca gcatgggaag gcctgcaatc ttggggtaaa gaaatgggcc ttcaaaaagg
    29041 aacaggtttc gattattttc aatctacaag tgaatctgag tatgcaacta atctcgatac
    29101 agcagtttca ggagggtatc aactgattta tggtatcggc tttgcattga aagatgctat
    29161 tgctaaagca gctggagata atgaaggagt taagtttgtt attatcgatg atattatcga
    29221 aggaaaagat aatgtagcca gtgttacctt tgctgaccat gaagctgctt atcttgcagg
    29281 aattgcagct gcaaaaacaa caaaaacaaa aacagttggt ttcgtgggcg gtatggaagg
    29341 aactgtcata actcgatttg aaaaaggttt tgaagcagga gttaagtctg ttgacgatac
    29401 aatccaagtt aaagttgatt atgctggatc atttggtgac gctgcaaaag gaaaaacaat
    29461 cgcagcagct cagtatgcag caggtgctga tgttatttac caggcagcag gaggcactgg
    29521 agcaggtgta tttaatgaag caaaagctat taatgaaaaa cgtagtgaag ctgataaagt
    29581 ttgggttatt ggtgttgacc gtgatcaaaa agacgaagga aaatacactt ctaaagatgg
    29641 caaagaagca aactttgtac ttgcatcatc aatcaaagaa gttggtaaag ctgttcagtt
    29701 aatcaacaag caagtagcag ataaaaaatt ccctggagga aaaacaactg tctatggtct
    29761 aaaagatggc ggtgttgaaa tcgcaactac aaatgtttca aaagaagctg ttaaagctat
    29821 taaagaagcg aaagcaaaaa ttaaatctgg tgacattaaa gttcctgaaa aatagtgttt
    29881 aagaacaaga agcgcgacct ttgtcggtcg ctctttttaa actgagataa tttttatctc
    29941 agtaaagttt gtgataggtg atagatcata ggctttactg aaataaaaga ttgcattgaa
    30001 aggaaatgat cctatgacac agcatgtcat tgaaatgaga gaaatcacga aaaaatttgg
    30061 tgattttgtt gcaaatgacc acattaactt aaatgtcaga aaaggtgaga ttcatgcctt
    30121 attgggtgaa aatggggcag gtaaatcaac tttgatgaat atgctagctg gtctcttaga
    30181 gcctactagt ggagaaattg tcattaatga caagccagtt caaattgatt cgccatctaa
    30241 atcagctaag cttggtatcg gaatggtaca tcaacatttc atgttagtag aagccttcac
    30301 ggttgctgag aatattattt taggaaatga agtagtaaaa aatgggtgct tagatcttaa
    30361 ccaagcatcg aaagatatta aggtactatc cgaaaaatat ggccttgcaa tcaatccatc
    30421 agctaaagtt tctgatattt cagtaggagc acaacaacgt gttgagattt tgaaaacctt
    30481 gtatcgtggt gcagatattc ttatctttga tgagccaact gccgttttaa cacctgcaga
    30541 aattaaagaa ttgatgacta tcatgaagaa tctcgtgaaa gaaggaaaat caattatttt
    30601 aattacccat aaactggacg agattcgtgc tgtcgccgag cgtgtcacag ttattcgtcg
    30661 tggtaagagt atcgaaacag ttgatgtggc aggagcaact tcacaagatt tagctgagat
    30721 gatggtggga cgctcggttt cctttacaac tagcaaaaaa gctgctgaac caaaagatgt
    30781 ggtcttatcc atcaaaaatc ttgaagtaga tgaaaataga gggattccag cagttaaggg
    30841 gctatctcta gatgtccgag ctggtgaaat tgtagggatt gctggaattg acggaaatgg
    30901 tcaatctgag ttaattcaag ctattacagg tttacgtaaa gtcaaatctg gaagtattat
    30961 gattaaaaac aaagaagtca ctcatttgtc ttcacgcaaa attactgagt tatcagtggg
    31021 tcacgtgcca gaagatcgtc atcgagatgg tttgatacta gatctttctt tagctgaaaa
    31081 tacagcctta caaacctatt acaaacaacc tctaagtcaa aatggaatct taaattatac
    31141 taaaattaat gactatgctc gtcaattaat gaaagaattt gatgttcgcg gagccaatga
    31201 gctagtacca gctagaggat tttctggtgg taaccaacaa aaggccatta ttgctcgtga
    31261 agtggaccgt gatccagatt tacttattgt tagccaacca acacgtggtc ttgacgtagg
    31321 agctattgaa tatatccata agcgtttgat taaagaacgt gataaaggta aagctgtcct
    31381 cgttgttagt tttgaacttg atgaaatatt gaatttatct gatcgaattg ctgttgttca
    31441 tgatggtaaa attcaaggta tcgtattgcc agaaaatacc aataaacaag aattgggtat
    31501 tttaatggca ggtggttcaa ttcataagga ggaaggtcat gtctaaaaat gcgcaaaaaa
    31561 ttgctgtacc attgatttct gtcttgctag gatttttact tggcgctatt attatggtta
    31621 tctttggtta tgatcctatc tgggggtatg aaggtttatt ccaaattgcc tttggtagtg
    31681 ttaaaaatat tggagaaata ttccgttcaa tggggccatt gattttaata gcgcttggct
    31741 ttactgttgc tagccgtgca ggcttcttta acgttggttt gtctggacag gctttagctg
    31801 gatggatttc agcaggttgg tttgcactct taaatccaga tatgcctcgt ccattattga
    31861 ttcttatgac agctcttatc ggaatgattg ctggtggtat tgctggagct attccaggga
    31921 tacttcgtgc ttatcttggt acaagtgaag ttatcgttac gattatgatg aactacatca
    31981 ttttatatgt cggaaatgct attgttcaac gtggttatcc tgagagtgtc aaacaaagta
    32041 ttgattcaac tattcaagtt agtgataatg ctagttatca aacgcattgg ttatcagccc
    32101 taactaataa ctctcgtatt aatattggta ttttctttgc cattattgcc attgccctaa
    32161 tctggttctt gcttaacaaa actactttag gttttgagat tcgttcagtt ggtctcaacc
    32221 cacatgcttc agaatacgca gggatgtcat caaaacgtac tatcatttta tcaatgatta
    32281 tctctggagc tcttgcaggt cttggaggag tagttgaagg tctaggaacc tttgaaaatg
    32341 tttttgtaca aggatcttct ttagcagttg gttttgacgg tatggcagtt agcttattag
    32401 cagcaaattc accgcttgga atatttttct catcattcct atttggtgtc ttaaatattg
    32461 gtgctccagg aatgaatatt gcaggcattc caccagaact tgttaaagta gtaactgcat
    32521 caatcatttt ctttgttggt tcacattacc tcattgaacg ttacattatt cgccctaaaa
    32581 aattagtgaa aggtggtaaa taagatgagt ttagtaacaa tttttgcctt attggtgtca
    32641 tctatgctta tttatgcaac accactcatt ttcacaagta ttggaggaac tttctcagag
    32701 cgctcaggcg ttgttaatgt tggtttagaa ggtatcatgg tcatgggagc gttctctggg
    32761 attgttttca acttagagtt tgctgaaaca tttggaaaag caacgccttg gatagccgtt
    32821 ttagtcggtg gtattgttgg tttgattttt tctttgattc acgctgtagc aaccatcaac
    32881 tttcgagctg accacattgt cagtggtaca gtgttgaact tgttagcacc ttcttttgct
    32941 gtcttcttgg ttaaagctat gtacggtaag ggacaaacag acaacattca acaatctttt
    33001 gggaagtttg atttcccagg tttatcacaa attcctgtga ttggtgatat tttctttaaa
    33061 aatactagcc ttattggtta cttcgccatt gctttttcgt tctttgcttg gtttttgctt
    33121 tataaaacca ggtttggtct gcgtttacga tcagttgggg aacaccctca ggcagctgac
    33181 acacttggta ttaacgtcta tttgatgaaa tactatggtg tgatgatctc aggttttctt
    33241 ggtggaatag gtggagctgt ttatgctcag tcaatctccg ttaactttgc tgtaacaact
    33301 atcttaggtc ctggatttat tgctttggca gctatgatct ttggtaaatg gaatccagtt
    33361 ggcgccatgc tatctagtct tttctttggc ttatcgcaaa gtttagccgt tattggtgct
    33421 caattaccat tactagaaaa gattccaacg gtttacttac agattgcacc ttatatggta
    33481 acgattatta ttttagctgc tttctttggt caagcagttg caccaaaagc tgatggaatc
    33541 aactatatta aatctaaata acgttatatt agtagtaagc ctcttagtga ggtttaacag
    33601 cactcctaaa gcaagatagt taggtctagc tttaagggtg cttttttggg acttaccgca
    33661 agcgtttgga ttgccagtca ccatcacaat agaagcaaga atagactaaa aagaactaag
    33721 tcagcggctt agttcttttt gcttgtcttt cgtttttact gatagctctt aagttgtttt
    33781 agccaacaaa ggtattgatt tctgtttcaa tgttagcaat tttttcttga gcgatgtcaa
    33841 gagtatcacc tattgtggca atatagaact tgatttttgg ttcagttcct gaagggcgaa
    33901 ctgcaatcca tgaatcatca gctaacgtgt acttcaaaac attacttgga ggagttgtta
    33961 agttagatat tgttccatca tttttagtag cggtttgttt ttggaaatct tctaataata
    34021 caatatcagt attgttaaat tgttttggac cattttcacg gaatttgtcc ataatcttct
    34081 taatttcagc agctccatcc acacctgaaa gtgttactga aatcgttttt tcagcaaaat
    34141 agccatattc tttgtaaatt tcatcaatac catctgcaag tgttaaacca cgcgaacggt
    34201 aataagctgc gatctcagca accaaaagaa ctgcttgaat agcatcttta tcacggacaa
    34261 atggtttaat aagatatccg aagctttctt caaaaccaaa catataggtg tggttatgtt
    34321 tttcttcaaa ctcttgaatt ttctcagcga tgaatttgaa tccagttaaa acgttaaaca
    34381 ttgtagcgcc ataactttcg gcaatttttg ttactaattc tgttgatacg attgatttag
    34441 cgagagcagc attttctgga agtgttcctg ctgttttatg agcttccagg atgtatttcg
    34501 caataatcgc tccaatttga tttccagaaa ggtttttata agaaccatct ggttggcgaa
    34561 tttcaacgcc taggcggtct gcatcaggat cagtagctac taagacatct gcatcaactt
    34621 ctcgtccgag ttcttcagca agagcaaagg cagcttggct ttcagggtta ggcgatttaa
    34681 cagtagaaaa gtctggatct gccttagctt gtgattcgac tacaacaaca gattcaaaac
    34741 cggcttgagc aagggcacgg cgtgtcaaca tctcaccagt tccgtggaga ggagtataga
    34801 caattttcat gtcttttcca aagttattga ttaaatcttg attgatatta acatctttaa
    34861 cctctctaag atattccata tcaacatctt caccgataac ttgaataaga cctgatgatt
    34921 tgctttcatc taaatcagca agttcaactg caaatggatt ttcaatagca cggataaagt
    34981 ctgttagtgc atcagcatca gctggtggta gttgacctcc gtcttgtcca tagactttat
    35041 aaccattgaa aggtgctgga ttgtggcttg cagtaaccat aataccagca taggcattta
    35101 aatggcgaac agcaaaagag agttctggtg ttggacgaag agcttcaaat acgtaagatt
    35161 taatcccatg ctgtgccaag acctgtgcag actcaaaagc aaattctgga gagaagtggc
    35221 gcgaatcata agcgatggct acaccatgtt ttttagcttc ctctccctta gtttcgatta
    35281 attttgctaa tccttcggtt gcttgacgga caacataaat attaatacga tttgtaccag
    35341 caccaatata accacgcata ccagcagtac caaattctaa gtttgtgtaa aaagcatctt
    35401 cttttgtttt ttcatccatt gatagtaatt cttgacgaag atagtcagga agctgctcaa
    35461 aatctaacca tttttgaaaa ttttctgtgt aagtcattga aatactcctt caatatttat
    35521 aaacgctttc attatatcat tttgaaggaa aaaaaacact aattatttgc agctgtttac
    35581 aaaacttttt ccatattttc aagtagctaa tgtaagaaag ttaaaataac caagttttta
    35641 aaagagaaaa actcggttat tgtgaaagct aattaccttt ttagtcttga tagtgttgga
    35701 ataagtacaa aggtaataat agctgagatg atcatctcaa caatagcgtt tgatgagatg
    35761 atggctgtta gtagtgcttt aatatttcca tcaaaaactg tcgcgaaaaa aataaatatc
    35821 ccagataata caaatatagt atttgttaga gaaccaatga taccggatac tattaaacca
    35881 aactgattag gaatcaattt atagcaataa taaggggtaa taccaatcaa aatacgtggg
    35941 ataatagcaa ttattaaaga agcgaaagtt ccatgatcga caaaaggtga aaagagataa
    36001 tttgtaggca gtagaatgat ggtatttgta atgacactta taatccccat taatcctcct
    36061 aaaatagcac ctatccttgg gccatataag acagaagcaa taataactgg gatatggaca
    36121 agcgttggtt tgattggaat aggccaaata ttaaagacaa gactgctcac aaaatgaatg
    36181 actaacatga tggcaaaaaa gatagcaata cgagaaatat ctgctgattt acggtgattc
    36241 attttactct ccaaaaaata taatacttaa ttctaaaaag gtgatcagaa aaacgactgt
    36301 taaagtttcc aaatcgtatc aatagtagct aagataacat caatatcagc caaagcacca
    36361 ggtcctttat ctccacaggc taataagcta gattttggag gaatttctgt aaaccctatt
    36421 gtcgataagc gtttaatatt ttcttgggta atagggtttt gatacatttt ggtattcatg
    36481 gctggggcta ttaattttgg tgtggtagct ggtagggcaa gagcaacact tgttacaata
    36541 ttatcagcga atccatatgc tagatgagca atagtattag cagaggcagg tgcaacaata
    36601 aataaatctg tgcgttttgc taattcgata tggttgataa cttttgggtc atgctcatcc
    36661 ataacatcta aatgaatagc attttttgat aacacttgca gggttaatgg tgtaataaat
    36721 tgtgtagcag cctgagtcat tatgatgtga acatcatagc caattttagt taattgactc
    36781 gtaagatctg ctgctttata ggcagatata ctaccagaaa cagctaaggt aatatgttta
    36841 gtcatgtttg gtcatccttt catataataa gtcagcaatt gcctctttgg tatctgcaga
    36901 tgcaacttcg ttattggata tcaaaagagc cttgtgcata cctgtttgga tatcaactaa
    36961 atcattggcg agaatataat cagcatggtt tttagctaga cttgctctgg ctactttaat
    37021 taactcttct tgaggcacat taactagaag tttaaagccg actagtttaa tattaggatt
    37081 ccactgttta acataagaga taactttggg tgtttttttc aaaaaaagaa cctgataatc
    37141 agatgcagat gagattttcg gctcactgtt atgttcacat agaaaacaat taaggttatc
    37201 agcttgagag acttgttcca aatcagtcat gtagactgga gtatagtcag aaacagccat
    37261 actatgaatt agaatgtcat ggtgaggaac ctgatctttg agggcagcca ttaagtcgtt
    37321 aactgtttcc acttcaataa tccttaggag cttatttggc aggggtttag tggctgtttt
    37381 agtagtgact agggtaacgt catgatgata ttgtaaaaat cgctctgtga ttaattttcc
    37441 aagttgccca gttgagtgat tagtgatacc tcgtactgca tctataggtt cagttgttcc
    37501 acctgatgtt attattaatt tcatagtcat attttatcaa aaaaggagct gatagtaaac
    37561 ggaagataat gacgccagac attgtcttgc gtttgtatta atatggaata gtaatagatt
    37621 tctagtaatt aaaacgaagt tctggaaagc aagacactat agtagctaaa atctaaattt
    37681 taaggtaaaa tagagcaagc ggtttcaagt gtgaaaggaa gaattgtgat aactattaga
    37741 gatttagcgt cactgccgat ttttgtttac ggtgatggaa ataaaaaagt tccaggagct
    37801 ttgagtcctt ttgtgtggac agaggtgttt ttaaaaacta tcaatcaaga acctaatcaa
    37861 ctgatcttac atatttggcc gatgacaaga acagttatat tagggatgtt agatagacag
    37921 cttccctact ttgagctggc taaaacagag attggcaata atggttatgt tcctgttact
    37981 cgtaatattg gcggtttggc tgttgtcgct gatgatggga ttttgaattt ttcgcttgtt
    38041 attccagacc atttttcgga atctatcagt attagtaatg cttatttaat aatggttgat
    38101 gtcattagag agagtttttc ggattattat cagcgtattg aatatcacga aattaaaaat
    38161 tcttattgcc ctggtaattt tgatcttagt attgcaggcc gcaagtttgc aggtatcgcc
    38221 cagcgtcgta tcaaaaaggg aattgttgtg tctatttact tgagcgtttg tggggatcaa
    38281 gcagcgcgtg gtcaactcat caaggatttt tatgaggccg gaacacaagg agaagttacc
    38341 aaggttaatt accctcaaat tgatccagaa tgcatggcaa ctctttcaga actccttgag
    38401 acgcctttta cagtggcaga agtccttgaa cgtcttcgat tgaccttgcg acagcttggc
    38461 ttttccctca cggaaaaaag tccagatcaa gccttactga ctaattttga tgctgtctat
    38521 gaacggatgc agctagaagt cgtcagaaag gaaggcaaat gaaagaaaga tttagtcctt
    38581 tatttgagcc tttgactttg ccaaatggga gtcaattgga taatcgcttt gttttgtctc
    38641 ctatggtgac caactcatcc acaaaagacg gttatgtcac ccaagatgat gtatcctatg
    38701 ctttgagacg agcagcttct gcccctctac aaattacagg agcggcctat gtggatcctt
    38761 atggtcaatt gtttgaatat ggttttagtg ttacaaaaga tgccgatatt tctggcttga
    38821 aagagctagc tcaagcgatg aaggcaaagg gagctaaagc tgtcttacaa ctgacccacg
    38881 ctggccggtt tgctagccat gccttgacta aatatggctt tgtctatggg cctagttaca
    38941 tgcagttgag atctccccaa cctcatgaag tcaaaccttt aacaggtcag caaatagagg
    39001 aattgattgc agcttacgct caggccactc ggcgggcaat tcaagcaggc tttgatggcg
    39061 tggaagtatc ttcggcgcag cgcttattga ttcaaacgtt cttttcgact ttttcgaaca
    39121 agaggacaga tagttatggt tgtcaaacac tttttaatcg ctctaagtta actttagctg
    39181 ttttgcaagc tgttcaacag gtgattaatc aagaagctcc tgatgggttt atttttggat
    39241 ttcgtgctac accagaagaa actaggggaa atgatattgg ttatagtatt gacgagtttt
    39301 tgcagttgat ggattgggta cttaatattg caaaacttga ttatttagcg atagcaagtt
    39361 ggggccgtca tgtatttcga aatactgttc gttctcctgg cccttattat ggaaggcgag
    39421 ttaatcaggt agttagagat tatttaagaa ataagctacc tgttatggct acagggggaa
    39481 tgaacacacc tgacaaagct attgaagcac tagctcatgc tgattttatt ggagtatcta
    39541 caccttttgt cgtggatcca gaatttgctc ataaaatcaa agaagggtgt gaagagagta
    39601 ttcatttaag gattcgacct gctgatttga aaagtttagc gattcctcag gcttctttca
    39661 aagatattgt acctttgatg gactacgggg aatcattgcc taaagaatca agaactttat
    39721 ttcgtagtct tactcacaat tataaggaga ttaaatgaaa gtcagtattc ttgattatgg
    39781 ggtgattgat aaagaaaaaa caccccaaga agctttatta gaaacgcgct gcttagcaca
    39841 agtagccgac aaacttggtt ttcatcgttt ttgggtagca gagcatcata atatttatgc
    39901 ctttgcaata agcagtccag agctgttgat gatgcattta gcagatcata ccaaacagat
    39961 tcggataggt tcaggaggta ttatgcctct acattatagt agctttaaga ttgcagaatg
    40021 gataatgact ttagaggcct tgcaccctaa tcggattgat ttaggtattg gcaactcttt
    40081 aggaactacg ttagttcaaa gagctctatc aagtatccat tgcaaagaca gctatagtca
    40141 ggttgtcaca gaattatacc agtatcttaa tcctgatcat ttgtcaccac taccgatttt
    40201 tgttaaccct aggggtaata cttaccctca gatttggacg ctaagcaata gtttggagac
    40261 agcagaactg gctggtcaat taggtttagg ctataccttt ggtattttcc cctatatacc
    40321 aaaagatcct ataacagaag ctaaaagagt atcggcacat tatagaaagg cttttaggcc
    40381 ttcaaagcta ttaaaaatcc ccaagttaat attagcagtt tttattgtcc tttctgatac
    40441 cgacgaggaa gcagaagctt tggctaaacc gttggacatt tggatgcttg gtcagcaaga
    40501 ttttaatgaa tttaagacct accctgatgt tgaggaggct agaaactatc acttaacaga
    40561 gaaacaaaga gaagccattg cagcaaatcg tagtcgcatg gttattggat cacctcacac
    40621 agtcaagaaa caattggata ggctgataga agcttgtcaa gctgacgaat tattagccat
    40681 ccccttagtt cctgaatttg ccaatagaca acgaacctta gagctgctag cagacttata
    40741 cgtgaccatt taaccttacc aaaaacaatt ccctaaactg aaaggaaaga aacaatgaaa
    40801 aaaattgcaa attacttatt aattgagaaa acagatgacc gctataccat tagcatgaca
    40861 ccagaattac aagacgatat tggtactatt ggttacgctg aatttacgga taacgaccac
    40921 cttgccgttg atgacattat cttaaatcta gaggcttcta agaccgtcat gtctgtcttg
    40981 tcaccattgg ctggggcagt tgttgagcgc aatgaggcag ccacactaac accaaccctt
    41041 ttgaattcag aaaaagctga ggaaaattgg attgttgtgc taacagatgt tgatcaagca
    41101 gcttttgatg ccttagaaga tgcctagttc atttgattta ttaggggaaa tgattgatct
    41161 gttgcaaaca gagcagctta ctagttcttg ggcctgtccc ttgcctaatg ctttaacaaa
    41221 gcgacaagac ttgtggcggg cgctcattaa ccagcgccct gctttgcctt tatcaaaaga
    41281 ttacttgaat ctggaagatg cttatctaga tgattggcgt gcttcttttg ttcctgtatc
    41341 agtcaaggac tgccaaaaaa ctaattatac cagtcttttt ctttaccatg gggatattag
    41401 gtaccttgct gtggatgcta ttgtcaatgc tgctaatagt gagttattgg gatgttttat
    41461 ccctaaccat ggctgtattg ataatgcgat tcatacgttt gccggaagtc gtttacgctt
    41521 agcttgtcag gcaatcatga ctgaacaagg ccgtaaggaa gccattggcc aagcaaagct
    41581 taccagtgct tatcatctac cagcctctta tattattcat accgtagggc caagaattac
    41641 caaaggacga cacgtttctc cgattcgagc tgatttgtta gcacgttgtt atcgttctag
    41701 tcttgatttg gcggttaaag caggattgac tagcctagct ttttgctcta tttcaacagg
    41761 agaatttggc tttcctaaaa aagaagccgc tcagattgcc attaagacag ttttaaaatg
    41821 gcaagcagaa catccagaat ctaagacttt aactatcatt tttaatacct ttacatcaga
    41881 agacaaggcc ttatacgaca cttatttgca aaaggagaat aactgtgagt aattggacaa
    41941 cctatcctca gaaaaatctc acccaggcag aacaactggc ccaattaatc aaagaagcag
    42001 atgcacttgt agttggtatt ggagctggca tgtctgcagc tgatggtttt acttatatcg
    42061 ggccgcgttt tgagacggca ttcccagatt ttattgctaa gtatcagttt ttggatatgc
    42121 tgcaagctag cctttttgat tttgaagatt ggcaagaata ttgggctttt caaagtcgct
    42181 ttgtcgcttt gaattatctt gatcagcccg taggccagtc ttatcttgat ttaaaagaga
    42241 ttttggaaac caaagactat catattatta ctactaatgc tgataatgct ttttgggtgg
    42301 ctggttatga tcctcataac atttttcaca ttcaaggaga atatggtttg tggcaatgta
    42361 gccagcactg ccatcagcaa acttataagg acgatacggt catccgtcaa atgatcgcag
    42421 agcaaaaaaa catgaaagtc ccaggtcaac tgattcctca ttgccctgaa tgtgaggcgc
    42481 cttttgaaat caataagcgt aatgaagaaa aaggaatggt tgaagatgct gactttcatg
    42541 cccaaaaagc acgttatgag gcttttttat cagaacataa agagggtaaa gtgctttatc
    42601 tagaaattgg tgtgggacat acgacgcctc agtttatcaa acaccccttt tggaaacgtg
    42661 tctcagaaaa ccctaatgcc ttattcgtta ccttgaatca caaacattac cgcatccctc
    42721 tgtcaataag gcgacaaagc ctagaactga cagaacacat tgcacaattg attagcgcca
    42781 caaaaaccat ttaccaaaaa tcgtaaccaa agccaataga cggttaatta agagatatag
    42841 aaggagacta acatgtacct tattgaaccg attcgtaacg gtaaacgtat caccgatgga
    42901 gctgttgctc tagccatgca agtttatgtt caagaaaatc tatttttaga tgatgatatt
    42961 ttatttccct actattgtga tcccaaagtt gaaattggaa aattccagaa tgcggttgta
    43021 gaaactaatc aagagtattt aaaagagcat catatccctg ttgttcgccg agatactggc
    43081 ggtggtgccg tttatgtaga ttctggtgct gttaatattt gctacttgat taatgataat
    43141 ggtatttttg gtgattttaa aagaacctat cagcccgcta ttgaagccct tcatcatttg
    43201 ggagcaacag aggttgaaat gtctggacgg aatgacttgg ttattgatgg gaaaaaggta
    43261 tctggcgctg cgatgactat tgctaatggc cgtgtttatg gggggtattc ccttttgcta
    43321 gatgtggact ttgaagccat ggaaaaagca ttaaaaccta atcgcaaaaa aattgaatca
    43381 aaaggcatca ggtcagttcg tagtcgcgtt ggaaacatca gagaacattt agcaccacaa
    43441 taccaaggta ttacgattga agagttcaaa gatttaatgg tttgccaatt gttacagatt
    43501 gagacgatta gccaagctaa gcgttatgat ttaacggaaa aagactggca acaaattgat
    43561 gctttaacag agcgtaaata tcacaattgg gaatggaatt atggcaacgc gcctcaatac
    43621 cgttaccata gagatggccg ttttaccggt ggaacagtag atattcattt agatattaaa
    43681 aaaggctata ttgcagcttg tcgcatttac ggagattttt ttggcaaggc agacattgca
    43741 gaacttgaag gtcacttgat aggaacacga atggaaaaag aggatgtctt agctaccctc
    43801 aatgcaattg atttagcccc ttatttaggt gcgattacag cagaagaatt aggagatttg
    43861 atattttctt gataaaaaag
//
LOCUS       DAUVUY010000005        42901 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.005, whole genome shotgun sequence.
ACCESSION   DAUVUY010000005 DAUVUY010000000
VERSION     DAUVUY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42901
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.005"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            178..2010
                     /gene="lepA"
                     /locus_tag="VOX58_000264"
     CDS             178..2010
                     /gene="lepA"
                     /locus_tag="VOX58_000264"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HES5877540.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDAKK"
     gene            2178..3400
                     /locus_tag="VOX58_000265"
                     /pseudo
     CDS             2178..3400
                     /locus_tag="VOX58_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164232585.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            3586..4023
                     /gene="msrB"
                     /locus_tag="VOX58_000266"
     CDS             3586..4023
                     /gene="msrB"
                     /locus_tag="VOX58_000266"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HES5877541.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDDFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYEQMEKEGYAQWLTLFDET"
     gene            4153..5172
                     /locus_tag="VOX58_000267"
     CDS             4153..5172
                     /locus_tag="VOX58_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HES5877542.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWYLGRLFPIIGAPVFAILLGMLL
                     ALFYHHRDKAKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLLIIISTIATALL
                     VAYGSQKWLRLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNDGFATFAGTAVNDTSSVTATATAWDALHHSNTLDDATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILFFLLASLITTLMTSFGVS
                     ADVFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            5379..5804
                     /locus_tag="VOX58_000268"
     CDS             5379..5804
                     /locus_tag="VOX58_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HES5877543.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDNFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDDIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            5823..6314
                     /locus_tag="VOX58_000269"
     CDS             5823..6314
                     /locus_tag="VOX58_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HES5877544.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            6331..7140
                     /locus_tag="VOX58_000270"
     CDS             6331..7140
                     /locus_tag="VOX58_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HES5877545.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            7137..7964
                     /locus_tag="VOX58_000271"
     CDS             7137..7964
                     /locus_tag="VOX58_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HES5877546.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            8100..9749
                     /locus_tag="VOX58_000272"
     CDS             8100..9749
                     /locus_tag="VOX58_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HES5877547.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDDLGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQDLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            9753..10541
                     /locus_tag="VOX58_000273"
     CDS             9753..10541
                     /locus_tag="VOX58_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5877548.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            10535..11581
                     /locus_tag="VOX58_000274"
     CDS             10535..11581
                     /locus_tag="VOX58_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HES5877549.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            11694..13091
                     /locus_tag="VOX58_000275"
     CDS             11694..13091
                     /locus_tag="VOX58_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HES5877550.1"
                     /translation="MAYQTIYPYTNEVLHTFDNMTDQDLADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGVKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            13193..14989
                     /gene="uvrC"
                     /locus_tag="VOX58_000276"
     CDS             13193..14989
                     /gene="uvrC"
                     /locus_tag="VOX58_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HES5877551.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLKIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            15174..15776
                     /locus_tag="VOX58_000277"
     CDS             15174..15776
                     /locus_tag="VOX58_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HES5877552.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            15901..17310
                     /gene="pepV"
                     /locus_tag="VOX58_000278"
     CDS             15901..17310
                     /gene="pepV"
                     /locus_tag="VOX58_000278"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HES5877553.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDRSADDQHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLDVLEAALEQFLSEYGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGINGATLLAKFLNQFTFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFNFDSQSADNTIALNFRYPKGTDAATLKSGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(17378..18754)
                     /gene="mnmE"
                     /locus_tag="VOX58_000279"
     CDS             complement(17378..18754)
                     /gene="mnmE"
                     /locus_tag="VOX58_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HES5877554.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQGSNRI
                     ILLNKTDLEQKIELEQLPADLISISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     misc_feature    18924..19059
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            19088..19588
                     /locus_tag="VOX58_000280"
     CDS             19088..19588
                     /locus_tag="VOX58_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HES5877555.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            19653..20018
                     /gene="rplL"
                     /locus_tag="VOX58_000281"
     CDS             19653..20018
                     /gene="rplL"
                     /locus_tag="VOX58_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HES5877556.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
     gene            20277..20876
                     /locus_tag="VOX58_000282"
                     /pseudo
     CDS             20277..20876
                     /locus_tag="VOX58_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            21051..21376
                     /locus_tag="VOX58_000283"
                     /pseudo
     CDS             21051..21376
                     /locus_tag="VOX58_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            21367..21641
                     /locus_tag="VOX58_000284"
                     /pseudo
     CDS             21367..21641
                     /locus_tag="VOX58_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            21690..21899
                     /locus_tag="VOX58_000285"
     CDS             21690..21899
                     /locus_tag="VOX58_000285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877557.1"
                     /translation="MTDRVMKKPIRGRQLSKRDLYDEEFFRTHFAKIEIESRLEFLLN
                     RANSLEELLLKAKEVNLTIDLKQKM"
     gene            22164..22861
                     /locus_tag="VOX58_000286"
                     /pseudo
     CDS             22164..22861
                     /locus_tag="VOX58_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
     gene            23012..23698
                     /locus_tag="VOX58_000287"
     CDS             23012..23698
                     /locus_tag="VOX58_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5877558.1"
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFK
                     GYDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKP
                     FSLKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYR
                     EYEILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKT
                     IRGIGYKWDV"
     gene            23691..25037
                     /locus_tag="VOX58_000288"
     CDS             23691..25037
                     /locus_tag="VOX58_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HES5877559.1"
                     /translation="MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTS
                     HNVGEEFYIKDKQSIKQLNNYMKTLGLDYVVFDRKTDKAMEGKYLSKEFSLFNEVAEE
                     KNNLTFNSVHYDLYTNINYNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIV
                     VALTRFVKEISLNFKEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSL
                     IEREILEKQDLSFQIAALSHDIKTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISV
                     FEGYFNSLISYTRMLSEDRSVKLILVEKLLSELHFEVDDLLNINNIEFSICNRLIITS
                     FYGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLK
                     KGDKLFYTEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI"
     gene            25194..25334
                     /locus_tag="VOX58_000289"
     CDS             25194..25334
                     /locus_tag="VOX58_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HES5877560.1"
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
     gene            25449..27100
                     /locus_tag="VOX58_000290"
                     /pseudo
     CDS             25449..27100
                     /locus_tag="VOX58_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            27084..28328
                     /locus_tag="VOX58_000291"
     CDS             27084..28328
                     /locus_tag="VOX58_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HES5877561.1"
                     /translation="MTPQIKQIVDYIADSFINYEQIVAESENKVLINIESKYRNTFFD
                     KSLSHGVPSLILMYSSLYKVTKDERYLTFSNIYVEKLVDIISRDGIESPSLYAGTAGI
                     ALAIKEASISGKYYPKLLSSLNCLLKEQIKDKLVVSFSNIEKGIIEPYDYDMVNGFSG
                     ITNYLLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREAEFDLGIAHGITGPML
                     ILAKLKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMA
                     WCYGTPGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIY
                     DLIGKNYSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVELESIGILQGVS
                     GIVLFLLNPYSDEELLWQRIFI"
     gene            28347..31313
                     /locus_tag="VOX58_000292"
     CDS             28347..31313
                     /locus_tag="VOX58_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HES5877562.1"
                     /translation="MYKVFPTGMIRIPLQKQEFYNKDYEDLINDKVFMEQLLVASPSL
                     YDSVIDFDKCNEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTKGTTSNVQKV
                     SFIKKAVPDYSWIYSLVKSYEISNLEKLSLKINTAAFTQGDRYVLPFTVNESEEDRNI
                     SFSKPIKLLVEKCKTRYIKYEELIDVFKNNYPDIVSDMLESYIHDLVTNDFLISDLRP
                     PICNINSLDYLLSKLEEGTLCTDLTTLKKMIEDYNDLNIGEGRDLLLQIFDKMESIYK
                     YDNGGNYLKVDSLAEYEGGFNILSETARESIERLAEMFVKLNTNVKRRGGRKNSLEEY
                     QLKFIGKYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQMFEKKYEEAL
                     LNGGHIEFYSKDLDDFQTDQSNSLDSFELNFNIKIINNDVKLYLGANIGSGQAGRSFG
                     RFYGLSETVRETIKNLNHQNNTNVELSFVPKQIRLANVIQNYSDESYNTSFFTTSWDS
                     ENELRLEDIYIRYSDGKFHFTTIDGKNELKFTMNNMLNSDSQSRVLRLLVDLSEFEYG
                     LSHWSLFPWDILAQERVYIPEILFEDITITTAQWNLSAIREEIIHQKIFTGKKDIFNK
                     FRKTYKIPKDIILKYADNELRLDLSIDSDLEILFKNINKYRFVSLRGIEQGESIFKDN
                     LGNYNTEIVVPILKESNGINNTNDNVQFKNEVKKIYLPFCDWLFFKFYGPKERQEELL
                     NNWKKFWLNLPSELFSQSNMFYMRYNDTNDHIRIRINCDSIENNFSLYLSVVQDLLPQ
                     LIESCIISDIEVSSYKPEVNRYGGPHLISYAEEIFCKESILFLNNTIEFSDDERLVCA
                     TYLVLYYLNHFFKDKETRRSFLFENYTGKYKKDFKNLPIDLPIEYMKILNGVTSALDR
                     YDFFQEMDRYLMSYMKEYNRFGGTNDIYNTKFNLVGSFLHLSMNRLNGIDREFEEKVY
                     CFAYYTLNAQQYIEWE"
     gene            31319..32008
                     /locus_tag="VOX58_000293"
     CDS             31319..32008
                     /locus_tag="VOX58_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HES5877563.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESAILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIVLEKEYDGSENLEEL
                     FNNQILFEKRR"
     gene            32014..32763
                     /locus_tag="VOX58_000294"
     CDS             32014..32763
                     /locus_tag="VOX58_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HES5877564.1"
                     /translation="MVQIFLSNLFKIKNTSISKLILIFPIICVMLSLLFSALGGESIL
                     RLAAETTVNQWGVIWINVIIAVNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARVLLI
                     ALINLIISMILSLMLIVISFVLPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKI
                     NVYFAMIINIISPLIIGTSFSLLNKWYLFPYDWSLKLLEPMTRMRINSIPFGAEFVPD
                     YSQIFISLFLGIAFFILLTNLFAISFKKQVK"
     gene            32766..33488
                     /locus_tag="VOX58_000295"
     CDS             32766..33488
                     /locus_tag="VOX58_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HES5877565.1"
                     /translation="MKSYLCSNFLKIKNTSYLLIHILAALLFPLLLFFYWSQRGGLQS
                     RTVLFSYFQIVGVLLPFVASIVCIQLKNLEESSGKYKYLLGYSQSNYKPFIVELVFLW
                     LCYCIVLIISITIFILLLKTIGINVSLRLLILNSLIYIIFAYVTYLINHIISYIFSTG
                     VALGISMVGVIVAAFCETSLGDKVWFLIPWAWLLRISDTLYNQQKMAIVPLIVIFFVS
                     CTVALLHIRIFKSWNQDCLTSS"
     gene            33679..33897
                     /locus_tag="VOX58_000296"
     CDS             33679..33897
                     /locus_tag="VOX58_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5877566.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFSKLYNVSIDYLLGQTDYPYRFHNK"
     gene            complement(33968..34111)
                     /locus_tag="VOX58_000297"
     CDS             complement(33968..34111)
                     /locus_tag="VOX58_000297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877567.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            34198..34418
                     /locus_tag="VOX58_000298"
                     /pseudo
     CDS             34198..34418
                     /locus_tag="VOX58_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            34574..34795
                     /locus_tag="VOX58_000299"
     CDS             34574..34795
                     /locus_tag="VOX58_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HES5877568.1"
                     /translation="MKEAYDELVRIRYEFSNNLSIENDNISFENYMNTIYLRAYKQKV
                     QAVTYEIALPHHKLFIQYFGSKLLKGITA"
     gene            35120..35503
                     /locus_tag="VOX58_000300"
     CDS             35120..35503
                     /locus_tag="VOX58_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HES5877569.1"
                     /translation="MKVHTTLEKDENGNWYRKDQTKTPAGERLIELDDVTIVVLENWR
                     RNQVVNTDTDFIISRFGEPFCKSTICRVIKHKAQEVGVPVITGKGLRHSHASYLINVL
                     KKDILYVAKCMGHADKSTTLNTYSH"
     gene            35766..37001
                     /locus_tag="VOX58_000301"
     CDS             35766..37001
                     /locus_tag="VOX58_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HES5877570.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVDRTYCRRKILKKGSLVLERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(37117..38079)
                     /locus_tag="VOX58_000302"
     CDS             complement(37117..38079)
                     /locus_tag="VOX58_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HES5877571.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMFPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEIGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            38412..39689
                     /locus_tag="VOX58_000303"
     CDS             38412..39689
                     /locus_tag="VOX58_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HES5877572.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASDYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTGFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRQYWKKHIEKNVLLTH"
     gene            39724..40308
                     /gene="folE"
                     /locus_tag="VOX58_000304"
     CDS             39724..40308
                     /gene="folE"
                     /locus_tag="VOX58_000304"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HES5877573.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            40317..41117
                     /gene="folP"
                     /locus_tag="VOX58_000305"
     CDS             40317..41117
                     /gene="folP"
                     /locus_tag="VOX58_000305"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HES5877574.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWVDPGFGFAKSVQQNTELLKGLDRVCHLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            41124..41483
                     /gene="folB"
                     /locus_tag="VOX58_000306"
     CDS             41124..41483
                     /gene="folB"
                     /locus_tag="VOX58_000306"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HES5877575.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGEKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            41480..41980
                     /gene="folK"
                     /locus_tag="VOX58_000307"
     CDS             41480..41980
                     /gene="folK"
                     /locus_tag="VOX58_000307"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HES5877576.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEFYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            42130..>42901
                     /gene="murB"
                     /locus_tag="VOX58_000308"
     CDS             42130..>42901
                     /gene="murB"
                     /locus_tag="VOX58_000308"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HES5877577.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINV"
BASE COUNT        13935 a         6862 c         8805 g        13299 t
ORIGIN      
        1 agaggctaag cagatgattg atttaaaaag gaatcttttc aaaattgatg tccgggcaca
       61 aaaggatgaa gagaaagttt tcatgcgtac ggcgtggata atccgtaaga aaagataggc
      121 aaaggcctac cttttttgtt ataatagaag gaaagactaa atgtatagga aaatagaatg
      181 aacagtcaag atttaaaaaa acgtcaggag aagattcgta atttctccat tattgcacat
      241 attgaccatg gcaaatctac tctcgctgac cgtattttgg aaaagacaga gacggtttcg
      301 tctcgtgaaa tgcaggctca gttattggac tccatggatt tggagcgtga gcgtgggatt
      361 accatcaagt taaacgccat tgagcttaat tacacggcta aagatgggga aacctacatt
      421 ttccacctta ttgatacccc agggcatgtg gactttactt atgaagtatc gcgttcgttg
      481 gcagcctgtg agggagcgat tctagttgtg gatgcagcgc agggcattga agcacagacc
      541 cttgccaatg tttacctagc ccttgataat gatttggaaa ttttaccggt tatcaataaa
      601 attgacttgc ctgctgctga ccctgagaga gtccgccatg aagtagaaga tgtcattgga
      661 cttgatgctt ctgaggctgt tctggcgtca gccaaggctg gtattgggat cgaagagatt
      721 cttgagcaaa ttgttgagaa ggttcctgct cctacgggtg atgtggatgc gcctttacag
      781 gccttgattt ttgactctgt gtatgatgct taccgagggg taattttgca ggttcggatt
      841 gtgaacggta ttgtcaagcc tggcgataaa atccagatga tgtcgaatgg aaaaaccttt
      901 gatgttacag aagttgggat tttcactcct aaggcagttg gacgagattt ccttgcgacg
      961 ggagatgttg gttatgttgc ggcctctatc aaaacggtag cagatacccg tgtgggcgat
     1021 acggtgactt tagctaataa ccctgctaag gaagccttgc atggctacaa gcagatgaac
     1081 ccgatggtct ttgcagggat ttatccgatt gaatcaaata aatacaatga cttgcgtgaa
     1141 gcgcttgaaa aattacaact gaacgatgcc agtttacagt ttgaacccga aacgtcacaa
     1201 gcgcttggct ttggttttcg atgcggtttt ttaggcttgc ttcacatgga tgtgattcaa
     1261 gaacgtttgg aacgtgaatt taacattgat ttgatcatga cagcaccgtc cgtggtgtat
     1321 cacgttcaca caacagatga ggacatgatt gaagtgtcta acccttcaga atttccagat
     1381 ccaacacggg tggcttttat tgaagagcct tatgttaaag cgcaaatcat ggtgccacaa
     1441 gagttcgtag gggctgttat ggaattatcg cagcgcaagc gtggtgattt tgtgaccatg
     1501 gattacattg atgataaccg tgtcaatgtg atctaccaaa tcccgctggc tgaaattgtc
     1561 tttgatttct ttgataaatt gaaatcctca acgcgaggct atgcgagttt tgattatgac
     1621 atgtcagaat accgcaggtc acaattggtg aaaatggaca ttcttttgaa cggtgataag
     1681 gtcgatgcgc tcagctttat tgttcataaa gaatttgctt atgaacgcgg gaaaattatt
     1741 gttgagaaat tgaaaaaaat cattccacgt caacagtttg aagtgcctat tcaagccgct
     1801 attggtcaaa aaattgttgc gcgttcagac atcaaggccc ttcgtaaaaa cgttttagcg
     1861 aagtgttatg gtggtgacgt gtctcgtaaa cgtaaattgc tcgaaaaaca aaaggctggt
     1921 aaaaaacgga tgaaggctat tggttctgtt gaagtccctc aagaagcctt cttgagtgtt
     1981 ctttcaatgg atgacgatgc caagaaataa ccaagcctaa tcgcttagtc ttcagaggag
     2041 aactcttgtg ggtctacatg accaaaaaat ggttatctta tacaaaaaga actttacaat
     2101 catttgcaca taatgttata ataatattat agtgttatct cattattagg tgcgcctgca
     2161 tttaatctta ttaaacagaa agaggaaaca aaaactgtga acaaaacaaa acaaaaacaa
     2221 aacatagcct tctttgtcgc tatggcttaa cctcagcggc tgctcttttg ctgacctttg
     2281 gaggtgcaag tgcagttaag gcggatgaac aagaagagaa agctaaagtt agaactgaat
     2341 taattcaaga gttagctcag ggactagggg gtattgagaa aaaaattttc caactctagg
     2401 tgatgaagat ttagatcata cttatatgac aaagctatta acatacctac aggaacgaga
     2461 acaagctgag aatagttggc gaaaaagact actaaagggt atacaagatc atgcccttga
     2521 tggacaagac ggtcgaaacg gtgaaagagg ggaacaagga ccaacaggtc tcgctggtaa
     2581 agccggtgaa gctggagcca aaggcgaaac cggccccgct ggtccacagg gtccacgtgg
     2641 tgaacaaggc ccgcaaggtc ttccaggtaa agatggtgaa gctggtgctc aaggcccagc
     2701 aggtccaatg ggtcctgctg gtgagcgagg tgaaaaagga gaacctggta cccaaggcgc
     2761 taaaggtgat cgcggtgaaa ccggtccagt aggtccacgt ggtgagcgag gcgaagccgg
     2821 tcccgctgga aaagatggtg aacgtggtcc agtaggtcca gctggtaagg acggccaaaa
     2881 cggccaagat ggtcttccag gtaaagacgg taaggacggc caaaacggta aagatggtct
     2941 tccaggtaaa gacggtaagg acggccaaaa cggtaaagat ggtcttccag gtaaagacgg
     3001 taaggacggt caagatggta aagacggcct cccaggtaaa gacggtaaag atggcctccc
     3061 aggtaaggac ggtaaggacg gtcaaccagg taaaccggct cctaaaacac cagaggtccc
     3121 tcaaaaacca gataccgcac cacatacacc aaaaacccct cagatccctg gccaatcaaa
     3181 agacgtgaca cctgcccctc aaaacccttc taatagaggt ctaaacaaac cacaaacaca
     3241 aggtggtaat cagctcgcaa aaacaccagc agctcacgac acacacagac aattgccagc
     3301 aacaggcgaa acaaccaatc cattctttac agcagctgct gtagctatca tgacgacagc
     3361 tggagttgta gctgttgcaa aacgtcaaga aaacaactaa tcctcttttt gctacctaac
     3421 tgatggaaag taaaactcag agagaacctc aaggttctct cttttttgaa tcaagatagt
     3481 agctctaggt ataacgtctt gtctgaggtg ctgtgaacaa atcagttacc actcacctct
     3541 ttttttggta aaatgtaagc actaagacat caaaaggagt tttccatgga aacatcagat
     3601 gagttaaaac aacgcattgg tgatctttct tacgaggtca cccagcatgc agcaacagaa
     3661 tctcccttta caggcgaata tgatgatttt tttgagaagg gtatctatgt cgatattgtt
     3721 agtggtgagg tcttattttc atcactggat aaatttaatt ctggctgtgg ttggcctgcc
     3781 ttttcaaaac ccattgaaaa tcgtatggtc acaaaccacg atgactcttc ttatgggatg
     3841 agacgagttg aggttaaaag ccgtgaagca ggatcgcatt tggggcatgt ctttagtgac
     3901 ggccccaaag aagctggagg cttgcgttac tgtatcaatt cagcagctct taaattcatt
     3961 ccttatgagc agatggaaaa agaaggctat gcccagtggc tcacattgtt tgatgagaca
     4021 taaccgggtt tcaaaatgac tgaaaaagaa gcgtcagtga ttcccacaag cactggattg
     4081 gtgaggcgta cgggtcaatt ttacaagcct ttgtaaaagg tctataatag ggaatgaaaa
     4141 aaggaggtta gtatgtcaac tcacttaaga aaacttccag ggctgttact ttgcttgttg
     4201 ttagctcttc cagcctggta tttagggcgc ttatttccca ttattggagc acctgttttt
     4261 gctattcttt taggaatgtt gttagccttg ttttatcacc atcgtgacaa ggctaaagag
     4321 ggaattagtt ttacatccaa gtatatttta caaacagcag ttgttttgct tggttttgga
     4381 ttaaacctaa cccaagttat ggcagtgggc atgcagtctt tactgattat catttcaact
     4441 attgcgacag ctcttttggt agcttatggc tcacagaaat ggctgcgctt agatgtcaat
     4501 acagccacct tggtgggtgt agggtcttcc atttgtgggg ggtctgctat tgcagcgacg
     4561 gctcctgtca ttaaggcaaa ggatgatgag gttgctaagg caatttcagt catttttctc
     4621 tttaatatgt tagcagcttt gctatttcca agtttaggac aattactggg cttatctaat
     4681 gatggttttg ctacttttgc tgggacagct gttaacgaca cctcttccgt gactgcaacg
     4741 gccacggcct gggatgccct tcaccattcc aatacactag atgacgcaac cattgtcaaa
     4801 ttgactcgca ccttggctat tcttccaatt actttaggtt tatcccttta ccgagcgaaa
     4861 aaagagcacg acatcgttac agaagaaaac tttagcctta ggaagtcatt ccctcgtttc
     4921 atcctcttct ttttattagc ttccctcatc acgacattga tgaccagctt tggtgtttct
     4981 gccgatgttt tccattccct aaaaacctta tcaaaattct ttatcgtgat ggctatggcg
     5041 gctatcggtt taaatacaaa cctggttaaa ttaatcaaga cgggcggtca ggctatcctt
     5101 ttaggagcta tttgctgggt agctatcacc cttgtcagtt tagccatgca attaagtttg
     5161 ggcatttggt aatcagacac gagaaatcag tttgactgat ttcttttttt gtatcttatt
     5221 tgtgtagaat tgtctacatt ttaaagagaa tagtgcattt taaaaacgtt ttcatttgat
     5281 aaaataaacc tatcgttaaa ccagttcagt aaagacaact ggttgagata agcaattacc
     5341 gtttattcag cggtttttgt taatagagga ggataggaat gaaacgaaaa tttctcattg
     5401 ggagtcacgg cagactagcc agtggtttgc aaagctctat tgacatttta gcaggtatgg
     5461 gacaggcact tgaaaccatt gatgcttatg ttgatgatag tgactacact agccagattg
     5521 ataactttat cgctggggta gcagcagatg agcagggcct tatttttaca gatttgttag
     5581 gcggcagtgt gaatcagaaa atggtaaccg ctgtaatgaa cagtggcaaa gacaatatct
     5641 ttttaatcac aaacagtaac ttagccactt tattgtcgtt ggtgttttta aaaccaggcg
     5701 aagcgcttac taaagatgat attgtaaccg ttatcaatga atcgcaagtt cagttagtgg
     5761 atttggtccc tgagacaaat tcagaagatg acttttttga ttaatatcta ggaggaagac
     5821 aaatgattac tcaaattcgt gttgacgacc gtttgattca cgggcaagtg gcagttgtat
     5881 ggaccaaaga gttaaatgcg ccacttttag tagtggctaa tgatgaagct gcaaagaatg
     5941 aaatcacaca aatgacgctt aaaatggctg tgccaaacgg catgaaactg cttattcgtt
     6001 cagttgaaga ctcgattaag ctctttaatg atccacgcgc caaagacaag cgtatttttg
     6061 tgattgtcaa ttctgttaaa gatgcttgtg ccattgctaa agaagttcca gacttagaag
     6121 ctgttaatgt ggctaacgtg gggcgttttg acaaatcaga cccagcaagc aaagtcaaag
     6181 tgacaccaag tcttttactt aacccagaag aaatggcagc cgcaaaggaa ttagtcagtt
     6241 taccagaact tgatgtcttt aaccaagtct taccatcaaa taccaaggtc catttgagcc
     6301 aattagtcaa ctaaagagag gtaaaaaagt atgttagtac cagcaacaat ggcagcttta
     6361 gctgtattga tttgttttgg gggaaattat ttaaccggtc aaagcatgat ggaaagaccc
     6421 ctagtagttg gactggtcac aggtcttctt ttaggagata tgaaggtcgg tatcttaatg
     6481 ggggcctctc tagaagctct tttcctagga aatgtgaata ttgggggtgt tattgcagct
     6541 gagcctgtaa cagcgactgc catggcaaca acctttacca ttatttcaca tattgaccaa
     6601 aaagcagcca tgaccctagc tgttccaatt ggaatgttgg cagcctttgt ggttatgttt
     6661 ttgaaaaacg tcttcatgaa tatctttgct ccaatggttg ataaagctgc agcagctaac
     6721 catcaaggta aactagtgat gctccactat ggcacttgga ttatctatta cttgatcatt
     6781 gcctctattt ctttcattgg tattttagta ggtagtggac ctgttaatgc ctttgtggaa
     6841 cacattcctc aaaatctcat gaatggcttg agtgctgcgg gaggccttct tccagctgtc
     6901 ggttttgcca tgctgatgaa attattgtgg accaacaaat tagctgtttt ttacctattg
     6961 ggctttgtct taacagctta tttgaaatta ccagcggttg cagttgctgc cctaggagca
     7021 gttatttgcg tcattagttc ccaacgtgat ctagaattag acgcaattac tagaggtgca
     7081 attagtaaac aaacaacctt tgactctaaa gaatcagaag aggaggactt cttcgcatga
     7141 catcacaaga taatttgact aaagaagacc gtaaaatgct tcgctcagtc ttttggcgct
     7201 catggacgat gaatgctagt cgtactgggg caacacaata ccacgcagta ggggttattt
     7261 acactttact accagtcatc aatcgttttt ataagactga taaggataaa gcagaggcac
     7321 tggtccgtca tacaacatgg ttcaatgcca ccatgcacat caataacttt atcatgggtc
     7381 ttgtggcatc gatggaaaag aaaaacagtg aagatcctga ttttgatgct agtgctatca
     7441 ccgctgttaa ggcttcgttg atgggaccta tatctggagt tggagattcc ttcttctggg
     7501 gaattttaag ggttattgcg gcaggtatcg ggatttcctt ggcaagtgca ggctcagcca
     7561 tgggagctgt tgtcttctta ctcttgtaca atattcctgc ttttattatt cactactata
     7621 gcctttatgg tggttattct gtaggtgcag gatttatcaa aaaactttac gaatctggtg
     7681 gtataaaaat cgtcactaaa acctcaagca tgcttgggtt aatgatggta ggctcaatga
     7741 cggcttcaaa tgtaaaattt aaaacgattc ttacagttgc tgcgaaaggt gccaaagaag
     7801 cagcttctat tcaagattac cttgaccaat tgtttattgg tattgtacca ttaatggtaa
     7861 cactagcagc tttttggctt ctacgaaaga aagtgaatat caactggatt atgtttggta
     7921 tcatgttcct tggcattatc cttggacttt taggtatttg ctaataatct cctagcaata
     7981 aaggtaagta agacaagttt tcttgttttg cttacctttt tccttttaag atgatgagac
     8041 ttgttttgat gaaaaacgta gtagcattaa gaaatttgtt ataatgaaag gagcttaaga
     8101 tgcgaggtga acaggtggaa gaacatttta aaaaacaatt acaagatgat atttctagac
     8161 atttttcgta ccaatcgtta atgttatcat tgttgctaat tggtcttttt attatttttt
     8221 cattagcgcc acaacaatta ggcctctatc gtgatatcaa tgccactgca acccgttatc
     8281 accgtttgat tagcaaacaa gaagccttgc ttgatgactt agggaaaaat agcttactac
     8341 cttttttaaa taaaaacctc agcactgctg atttaagcaa gcactatttt catttgcgcc
     8401 atagcagcca aacgtcacca gaacttttac tattttcacc tagtcaagac cttttatttg
     8461 ctagtaaccc acatttagga aatgttttta gtaaatctgt ttatattcaa gaagtcttga
     8521 gagcagctca tcctccaaaa accttgttta aagttgctat ggatagtgaa gatggtcact
     8581 acttgatgat cattaagcca atgattgatc aaaaccaact aaagggatat gcttttttag
     8641 tgatgagtgg caaagatttt cttcatccta caaaaacact gacgtcagag ttggtcattg
     8701 ctgataagct ggacaatacg tttacgtttt ccaatcgtga gtttatagca tctagtctag
     8761 ataagatcaa cagtcagtat ttacaccatt attttgtttt tcaagataat cgagccttta
     8821 tcacaagaaa ggttgcctta caaggaggtc tttggcttta catgtatagg cccttgattc
     8881 caatggtatc ggtgatgtta ttttcactta tttcatcagc tgttatcttt gtgatcttgc
     8941 aacgtaaatc aagtggttta gctaaccgaa ttgcagctaa aaattcaaga gcaatcaatc
     9001 aaatggttag agatatgagt gcgatctctc ggcaagaaaa aagacgtatt gaccttgaga
     9061 gtcaagatga atttcaatat ttatctgatc aaatcaatca aatggtagag cgattgcaac
     9121 agttacatga taaaacgtta gatttggaaa ctcaaaaatt attatttgaa aaacggatgt
     9181 tagaagctca attcaatccg catttcctct acaatacgct agaaaccatt ttgattacga
     9241 gccattatga ttctgcccta acagaaaaaa ttgttatcca attgacaaaa ttgttgcggt
     9301 atagtctcac ggattctagc aaacctgtct tgctcaaaga tgatttaagt gtcatcgagt
     9361 cttatttagt gattaatcag gtgcggtttg aagaattgca atatagcatt aacctatccc
     9421 cagaccttga tagtcttgca gtgcctaaat tgttcttgtt acctttgata gaaaatgcca
     9481 tcaaatatgg cttgaaagaa cgacacgatg tcaagattaa cattgcttgt tattatcaag
     9541 atgaccatat tattttttct gtgcgagata atggttcggg aattgatgct catcaccaaa
     9601 aagtgattcg agagcaattg gaagctggag agtcacacca tgggttaatt aactcttatc
     9661 gtcgacttaa gtatcatttt tcagaagtat ccttggtttt tgatcaaggt gataaacagt
     9721 ttaatgttag ctaccatgtt aaggagtagt ctatgtacaa attagtcatt attgaagatg
     9781 aacatattat taggaaatgg ttgcgatacg ctattgatta taaagcttta gatattttgg
     9841 tcattggcga ggctaaagat ggcaaagaag gagctgtttt aatcaaagag agtcaaccag
     9901 atatcgtttt aactgatatt aacatgccca tcatgacagc ttttgacatg tttgaagcaa
     9961 caaaagatca aacttacgcc aaaattattt tatctggcta tgccgatttt ccaaatgcaa
    10021 gatctgctat acactacggt gtacttgaat ttttaacaaa accgattgaa aaagcagctc
    10081 tttgggaatg tcttcaaacc attattgcta aaatagaaaa gcaaaagggg tctaatcaaa
    10141 aaacagatgc ttgcgtctat attcccttac cccagatgac tgatcagatt cctgaagtag
    10201 tcaaagatat tttggagtgg gtacatgctc attttcaaga caagatcagc acgagccgcc
    10261 tagctcatga cttagggtat agtgagagtt atatctatca aaatatcaaa aagcatcttc
    10321 agatgccttt aagtgattac attaatcaat atcggattaa tcaggcaatt caattgatgc
    10381 aacaagaacc agatttaatg gtatatgaaa tagcacaagc tgtaggtatt tatgactatc
    10441 gctattttga tagggttttt aaaaagtatc taggacaaac tgtaaaggct tttaaagaag
    10501 agcattttat gaaggatacc gataggagta agccatgtta aaactcaaac gcaaatggct
    10561 attaagcttt ttgttagtaa taattattct agcaatcgtt aacattgcaa tgtatatatt
    10621 ctcatcgtca aaaaaagata gtgctaaaga attagttatc ttgactccta atagccaaac
    10681 tattttaaca gggactattc cagcctttga ggaaaagtat ggggttaaag taagattaat
    10741 ccaaggtggg acgggccaac ttattgatca attaggtcga aaagataagc cattaaacgc
    10801 tgatattttc tttggtggca attacactca atttgaaagc cataaagatt tatttgaatc
    10861 ctatgtttct ccgcaggttt ctactgtcat ttcagattac caattgccta gtcatcgtgc
    10921 aaccccatat acgatcaatg gcagtgtact gattgttaat aacgaattag caagaggact
    10981 tcatattacc agttatgagg atttgctaca accagcttta aaaggcaaaa ttgcttttgc
    11041 tgacccaaat agctcatcta gcgctttttc acaattaaca aacatcttac tggctaaagg
    11101 aggctatacg agctcagaag cttgggagta tgtgaaacgt ttattactgt ctatgaattc
    11161 tatcagagca acaagttctt cagatgttta cgaatctgtt gcagaaggta aaatgattgt
    11221 tggtttgacc tatgaagacc cttgcatcaa tctgcaaaaa agcggtgcaa acgtttcgat
    11281 tgtttatccg gatgagggga cagtatttgt tccgtcatca gtcgctatta tcaaaaaagc
    11341 aaaggcaatg aaagaagcta agcaatttat taattttatt ttatcatcag atgtacaaaa
    11401 tgcctttggg cagtcaacta gtaatagacc tatccgacga gatgctcaaa cgagtcatga
    11461 aatgaaacca ttagatgaaa ttattacttt aaaagaagat tacggatatg ttactagaca
    11521 taagaaaaaa atattgaata cctataatcg actacatgaa gcattagaaa gaggaaagta
    11581 gactctaggc ttgttgttgc tatactagta gtgttacaaa atagcgtagt cattttttga
    11641 aatcgatttc ataatctggt agactaagat tatcacgaaa aggagataat ataatggctt
    11701 atcaaactat ttacccttat acaaatgaag tccttcatac atttgataat atgaccgatc
    11761 aagatttggc agatgtcctt gaaagagctc accttctcta taaaaaatgg cgcaaggaag
    11821 atcatttaga agaacgtaaa gcccagcttc atcaagtggc taatattctt cgccgtgacc
    11881 gcgataaata tgctgaaatc atgaccaaag acatgggaaa actgttcaca gaagcccaag
    11941 gtgaagtaga tctttgtgcc gatattgctg attactacgc tgataaggca gatgaatttt
    12001 tgatgtctac gccactagaa acagattcag gtcaagctta ctatttgaaa caatcaactg
    12061 gtgttattct agctgttgaa ccttggaatt tcccttacta tcaaatcatg cgggtctttg
    12121 cgccaaactt tattgttgga aacccaatgg ttttaaaaca cgcttctatt tgcccacgat
    12181 ctgcccaatc ctttgaagaa ttagttcttg aagctggtgc tgaagcaggt agtattacca
    12241 atttgtttat ttcttatgac caagtatcac aagtgattgc tgataaacgt gtggttggtg
    12301 tctgcttgac aggttccgaa cgtggaggag ctagcattgc agaagaagct ggtaaaaact
    12361 tgaaaaaaac aacccttgaa ttgggtggcg atgatgcctt tattatcctt gatgacgcgg
    12421 attgggatca attagagaag gtgctttact tctctcgtct ctacaatgca ggtcaagtat
    12481 gtacctcatc gaaacgtttt atcgtgcttg acaaagacta tgaccgtttc aaagagttat
    12541 taacaaaagt cttcaaaaca gctaaatggg gtgatccaat ggatcccgaa acgacgttgg
    12601 cgccattatc atcagctcaa gctaaagcag atgttcttga ccaaattaag ttggccttgg
    12661 atcatggtgc ggagttggtt tatggtggtg aagcgattga tcatccaggt cattttgtca
    12721 tgccaaccat tattgctggc ctcacaaaag acaaccctat ttactatcaa gaaatctttg
    12781 gcccagtcgg tgaaatctac aaagtgtctt ctgaagaaga agcgattgaa gtggcgaatg
    12841 attctaacta tggccttgga ggtactatct ttagtagcaa tcaagaacat gcgaaagccg
    12901 tagctgctaa aattgaaact ggtatgtctt ttatcaactc aggctggaca agtcttccgg
    12961 aactcccatt tggtggggtt aaacattcag gctatggccg tgaattgagt gagcttggct
    13021 ttacatcttt tgtgaatgaa caccttatct atattccgaa taaaacgaat aatagcaata
    13081 ctaaagtttg atctagattg ttaaagaaga gtgtaacgcc ttaataaaca atcaaggcgt
    13141 tacactcttc ttttttaaag tcaaaatttt aaaaatttga taaactagta acatgaacga
    13201 actgattaaa cataaactag aactcttacc agacagccct ggctgttact tacataaaga
    13261 taaagaagga actattatct atgtcggtaa ggctaagaat ttgaaaaaac gcgtccggtc
    13321 ctattttcgt gggagccatg ataccaaaac agagttacta gtatctgaga ttgtagattt
    13381 cgaatatatt gtaacagagt cagataccga agcgcttttg ttggaaatta acttgattca
    13441 aaaaaatatg cctaagtaca acatcaagtt aaaagatgat aaatcttatc cctttctcaa
    13501 aatcacaaat gagtcttttc ctcggttagt gattacaaga tatattaaga aaaatgatgg
    13561 attgtacttt gggccctacc cagattctta cactgctaat gaagttaaaa agttattaga
    13621 taggattttt ccatttaaaa agtgtaaaaa tcctatcaat aaggtctgtt tttattacca
    13681 tttaggccag tgctgtgctc ataccatttg tcatacagac aaggcctatt gggatcgttt
    13741 aattgatgat gttaagcact ttttaaatgg taaagatgac aaaattattg aagatcttcg
    13801 ttctaagatg ttagcagctt ctgaagaaat ggcatttgaa cgtgccgctg aatatcgaga
    13861 tttgatttca ggtattgcca ccatgcgcac caagcagcgg gtgatgagca aggatttgca
    13921 agatagagat atctttggct attatgttga taagggatgg atgtgtgtcc aagttttctt
    13981 tgtgcgtcaa gggaaattaa ttcagcgtga tgttaacctt tttccctatt ataacgatgc
    14041 agaagaagat ttcttaactt atatggggca attttaccaa gataaacagc atttcatccc
    14101 taaagaagtc tttattccag aagctattga tgaagaactg gtagccgcta ttgtgccaac
    14161 caaaatcatc aaacccaaac gcggtgaaaa aaaacaactg gtagctttgg ctactaagaa
    14221 tgctcgtgtt agtctgcaac aaaaatttga tttattagaa aaagatatca aaaaaacgag
    14281 tggtgctatc gaaaatttag gtcagttact taagattgac aagcctgtgc gcattgaagc
    14341 ctttgataat tctaacattc aagggacaag tccagttgcg gctatggttg tttttgttga
    14401 tggcaaacct agtaagaaag attatcgtaa atttaagatt aaaacagtgg tgggaccaga
    14461 tgattatgcc agtatgagag aagtgctttt tcgaaggtac agtcgggtga aaaaggaggg
    14521 actgcaagct cctaacttga ttattgttga tggaggcgtt ggacaagtca atgtggccaa
    14581 agatgtgata gaaaaacaac ttggactcac tattcctgtt gcgggacttc aaaaaaacga
    14641 taagcaccag acccatgatt tgctttttgg aaacccactt gaagttgtac cgttgccgcg
    14701 ccgttctgaa gagtttttct tactgcatcg gattcaagac gaagtgcatc gctttgcagt
    14761 tacatttcat agacaagtgc gacgtaaaaa ttctttttca tcaacattag atcatatttc
    14821 agggcttggc ccaaagcgaa aacagttatt actaagacac tttaaaacca taacagcgat
    14881 tgcatcagct acctctgagg agattcaagc tttaggtata cctaaaacag tagttgaagc
    14941 aatacagcaa cagataacag acaataaaaa tgatagatcg tctccttaaa taagtgttat
    15001 gttttcagta ctgatgaggt aaaaaggcgc tacttgttac gcacgacatt tcagttcgtt
    15061 attttgaaac tttcagaaag ttgcgaagtg tagtgagttt atgattttaa aaactatttt
    15121 atggtaaaat aaaatggtat taaaaaaaac agatttagat aggaagtttt gaaatgaaat
    15181 ttctagagtt aaacaaaaaa cgccatgcca ttaagacctt taatgaccag ccagttgact
    15241 acgaggattt gcgaacagcc attgagattg caaccttagc acctagtgcc aataacattc
    15301 aaccttggaa atttgtagtc gttcaagaga aaaaagctga gttagcaaaa gggttacctc
    15361 tagcaaataa agtgcaggtt gaacaggctc aatatgtagt ggcgctattt tcagacactg
    15421 atttagcttt acgttctcgt aaaattgccc gtatcggtgt caaatcgtta ccagacgact
    15481 taattggtta ttatatggag actttgccac cacgttttgc tgcctttaat gaggttcaaa
    15541 ccggtgagta cttagctatt aatgcaggca ttgtggctat gaacttggtg ttatccttga
    15601 cggatcaaaa gattgcttcc aatattatcc ttggttttga taagtcaacg accaatgaaa
    15661 ttttagacat cgatccgcgt tttcgtccag aattattgat tactgttggc tatagtgacg
    15721 agaaaccaga accaagttat cgtttgcctg ttgatgaagt catcgaaaga agatagagtc
    15781 aggacaatta gttaaatgta atgtccagat gcgctacgga gcgaccgcta ttgttagact
    15841 gatactattt tggcttattt taagttaaac attatcccta aaacgaataa ggagagtata
    15901 atgacaacca ttgattttaa agcagaagtt gacaaacgta aagaagctat gctagcagat
    15961 ttagttgacc tattacgcat taattcagaa cgcgatgaca gatcggccga tgatcagcat
    16021 ccctttgggc caggtccagt taaggctttg gagcatttcc tcgcaatggc tgagcgtgat
    16081 ggctacaaaa cgcgtaatat tgataactat gcaggtgact ttgaatttgg tcaaggtgat
    16141 gaagtactag gaatctttgg ccaccttgat gttgtgccag ctggtagcgg ttgggataca
    16201 gacccttatg agccagtgat taaagacgat cgcatctatg cgcgtgggtc ttcggatgat
    16261 aagggaccaa ccatggcgtg ctactacgct ctgaaaatca ttaaagaatt gggtcttcct
    16321 gtgtctaaaa aagttcgttt cattgttggt accgatgaag aatctggctg gggggatatg
    16381 gattattact ttgctcataa tggcttgaaa aatccagatt ttggtttctc accagatgct
    16441 gaatttccaa ttatcaatgg tgagaaagga aatatcaccg aatacctcca tttcgctggt
    16501 gacaacaagg gcgcttttgt gcttcatcgc ttccaaggtg ggttgcgtga aaacatggta
    16561 ccagaatctg ccacagctgt cattacggca ccgcatgacc ttgatgtgct agaagcagcc
    16621 ttggagcaat ttttatcaga atatggtgtt aaaggctcta tgaaggctac tgatggtaaa
    16681 attgaagtaa ctattattgg gaagtctgct cacgggtcaa ctccagaagc tggaatcaat
    16741 ggtgccacct tgctggctaa attcctgaat caatttacct ttgaaggggc tgctaaagat
    16801 tatttacatg tggcaggtga agtgcttcat gaagactttg cggcagaaaa actaggcctt
    16861 gcttataccg acgaccgtat gggagccttg agcatgaatg caggtgtctt taactttgat
    16921 agtcaatcag ctgacaatac catcgccttg aatttccgtt atccaaaagg gactgatgcg
    16981 gcaaccttaa aatcaggatt ggaaaaatta ccaggtttaa ctaaggtaag cctttctgag
    17041 catgaacata cacctcatta tgtgccaatg gatgatgaat tggtagcaac cttgcttgct
    17101 gtttacgaaa aacaaacagg tcttaaaggc tacgaacaag ttatcggcgg aggaaccttt
    17161 ggtcgtttgc tagaacgtgg tgtggccttt ggagcaatgt tcccaggtga tgaaaacact
    17221 atgcaccaag ctaatgaata tatgccttta gaaaatattt atcgctcagc agctatttat
    17281 gctgaagcaa tctacgaatt aattaaataa gatgtttatg acagcaaaaa gagctggaac
    17341 agtgttccag ctcttttgtg atgattagag actgttgtta tttaccaaga cagaattgac
    17401 taaagagttg ggtaatgagc tcatcaggag cagcatcccc tgtgatttct ccgagaattt
    17461 cccaagttct ggttaaatca acctgtagta aatcaactgg catgccaagc gccaagccgt
    17521 cattgacagc ttctaaactc tggactgcct tttcaatcaa agaaatgtgg cgggcatttg
    17581 acaggtaagt ggcatcttgt tctactagtc cagcattatc aaagaagagc tggttgatac
    17641 gatcttcgat cagattaatg ttctggttgg ttaacaccga aatagaaata aggtccgctg
    17701 gcaactgttc caattcaatc ttttgttcca aatctgtttt attcagcaat ataatgcggt
    17761 tactgccctg actgagattt aacaaagccc ggtcttggtc agtcagtttt tcagaagcat
    17821 tcagcacaag aagaacaagg tcagcttctt gaagagcttt tttagaccgt tcaactccga
    17881 tttgttcgac cagatcatct gtctcacgaa taccggccgt atcgacaagt tttagaggaa
    17941 ctcccttgat attaacgtat tcttcaatga catctcgggt ggttcctgca atatcagtca
    18001 caatagcctt gtcttctcgc aacaaattat taagtaagct tgacttaccg acgtttggac
    18061 gaccaataat agctgttgat aagccctcac gcagaatttt accgcgttta gctgttcgaa
    18121 ggagattttc caaaaggctt tggaattctt gtgttttttc acgtagcaga gcagtggtca
    18181 tttcttccac atcgtcgtat tcagggtagt caatattaac ttcgacttga gctaaggtgt
    18241 tgagaatttc ttgtcgggtg tcattgataa gctgggaaag ggagccatcc agttgcttga
    18301 ctgcaatcgt catagccttg tctgttttgg cacgaataat gtccatgaca gcctcagctt
    18361 gagtcaaatc aacacgacca tttaagaagg cacgtttggt gaattcgcca ggctctgcca
    18421 ttctagcgcc ttggcgaatc aatagttgca agatttcatt tgtcacagca ataccaccgt
    18481 gcgtattgat ttcaaccaca ttttcacggg taaaggtttt gggggctagc atgactgaca
    18541 ccataacttc atcgataata gttcctgtct taggatcaat gatatggcca tagttaatgg
    18601 tgtgtgaggc gacttgctct aagttctttc ccttaaaaac agattgtgca atggcaagcg
    18661 catctgtccc ggatagacga acaatgccaa tagctccttc accaagaggg gttgaaatgg
    18721 cggtaattgt atcaaattct ttagttatac tcatgccctc tattttaaat caagtcctag
    18781 tagaaagcaa ggtattattt tgtatagaaa ataggactag caccattttg agtttagcta
    18841 atactaaaaa aataaatgta aaaaaatcat ttttttaata atttttattg acaatagtat
    18901 tataaaagaa tataatgata taggttttga taaaaaactg acctaagaca gcaggggagc
    18961 atgctcataa tattcctgcc gaggcacaaa acgtgattaa agaaaataac gtatttgtac
    19021 tttcgtgtct aggtttaggt gcgatttttt tgtacctagc ccaaaaataa aaacggaggt
    19081 aaaactaatg agtgaagcaa ttattgctaa aaaagctgaa caagtcgaac ttattgctga
    19141 gaaaatgaaa gcagcagcaa gtatcgttgt tgttgattca cgtggtctta cagttgatca
    19201 agatactgtt cttcgtcgtt cacttcgtga aagtggcgtt gagttcaaag ttatcaaaaa
    19261 ctcaattttg actcgtgcag ctgaaaaagc aggtcttgat gagttgaaag atgtattcgt
    19321 aggaccatct gcagtagcat tttctaacga agatgttatc gcaccagcta aagttatcaa
    19381 cgactttact aaaactgctg acgcacttga aatcaaaggt ggtgcaatcg aaggcgctgt
    19441 ttcttcaaaa gaagaaatcc aagcacttgc aacattgcca aaccgcgaag gaatgctttc
    19501 tatgcttctt tctgtacttc aagctccagt ccgcaacgtt gcatacgctg tcaaagctgt
    19561 tgcagaaaac aaagaaggcg ctgcttaaga cgtctcagag tagcttaagg ctacgcttaa
    19621 cattttaaat taattatttg gaggaaatca caatggcatt gaacattgaa aacattattg
    19681 ctgaaattaa agaagcttca atccttgagc ttaacgatct tgtaaaagct atcgaagaag
    19741 aatttggtgt aactgcagct gctcctgtag ctgctgcagc tgctggtggt gctgaagaag
    19801 ctgctaaaga ttcattcgac gttgaattga catctgctgg cgacaaaaaa gttggcgtta
    19861 tcaaagctgt tcgtgaaatc acaggtcttg gtcttaaaga agctaaaggt cttgttgatg
    19921 gagcacctgc taacgttaaa gaaggcgttg ctgctgcaga agctgaagaa atcaaagcta
    19981 aacttgaaga agctggagca acaatcactc ttaaataaga agcagatagc aatttgagag
    20041 cgaaatcccg atttatcagt cttttagagc ttatagcgat ttgaagaaac tgataaaatg
    20101 atttggtttt ttgccaaaaa tcctgccaaa aaaatctttg gcaggatttt tattttctat
    20161 aaaaattaag ctgagtagag tggttattct atctatatgt gtctagtaat aattttttta
    20221 ttttcaggag gaaaatcatg caaatgaatg aacagtcatc acgacaaatg gtgattgcca
    20281 tctatctcat ttattccaac tcaaaatgat agtactcctt ggctgttcca agtcgaaatt
    20341 gttagtgatt aagaaaacct acgagactat ggtttgatcg atgaaggcca gcagtctccc
    20401 agtgaaaaag ggcgtttggc taataagatt tatttaggaa agttggaaca tgatttcacc
    20461 ccagtcttaa gttcagacgg ggccagtctc aaaaacagac cagccccgtg ttaaattcag
    20521 cccctagtga gactgaaaag agtgaaactg aaggtagtga aactaaagag agtaatttag
    20581 tcattgagga cgaggaggaa agaaaggagt gcactagtgt gaaaaagact gagggtcact
    20641 ttactcgtca agttgatcat gtcaccaaat atgacaaaga ttatatttgg agtttggttc
    20701 attcccaatt acgtgaaggt ggtttatcac aagctgctag tgacttagtc atgagctatt
    20761 ttgaagaacg ctatgcttat gcgctggaac acatacggtt cgcaagaaca gctgaagcta
    20821 ttgcggaata tgtctttaat ggtgtcttat ccgaatggac caagcaacta aggtgacaag
    20881 aagcgaagtg agtaagatgt tttggtggct attattaggt gtttggggac taaggacaat
    20941 ttggttgatt attgaagtta tcactgcacc agaaatggaa gattatagtt gagtaaggac
    21001 gttacataag cagtaaggtc cttttatttt gtcagaaagg aatgagagat atgcagtttt
    21061 tagatttgtt tgcaggaatt ggaggctttc gattgggttt aattaatcag tgccatgagt
    21121 gtattggctt ttgtgagatt gataagtttg cgaggcaatc ttacaaagcc atctatgaga
    21181 ccgaaggaga aatagaattt catgacatta gacaagtcac agaccaagac tttagacaac
    21241 ttagagggca agtggatatc atctgcggtg gtttttccct tgtcaagcat tttcactcgc
    21301 aggcagacga ctgggatttg agggcactag gagaactctc ttctttgaga ttactcgagc
    21361 gaccaaacag ataaaaatca tacccataat cacatccaat caacgccatt gaccgtaatt
    21421 cagataaaaa attgatatgg aattatgcct tggaacgaaa cctccgtatg attttagacc
    21481 gtatttcaaa aatgactgga gcaaaaatta tcgagaagcg ttattcctac cgtgactatc
    21541 aaaaatatag gcagtctagt cataaatttg aattgaaaca acgtctttat tttttgatgc
    21601 agcagtcaaa gttctttgat gattttttag aaaaagcaga gtaattacat gttcatattg
    21661 attttagtca gaagcatagc agattcatga tgacggatcg agtaatgaaa aaaccaattc
    21721 gaggccgtca actcagtaaa cgagatttat atgatgaaga attttttcgt acacattttg
    21781 ccaagataga aattgaaagt cgattagaat ttttgttgaa ccgtgccaat tcattagagg
    21841 agctactatt aaaagcaaaa gaagtgaatc taaccattga cttaaaacaa aaaatgtaac
    21901 ttttatccta gaagaagata ctcaaaagat aagtttaggt cataaaaaaa taagcgataa
    21961 gaaattatat gatgtcaatt ttttcaagat tactttaaaa ataaggaagt cggtgtttca
    22021 gatcgattag aaagtctaca gaaacagtac atgcttttca agaagaacga gataaagagg
    22081 aggtatccac tgaagagagt aaggaagtct tcaagaaata caaggggcaa cgagatgcca
    22141 tccatgagtt tgaagttgaa cttgtggaac atcaaattga gaggttaatt gctgaggaca
    22201 tttatattaa ggtgtctttt ggtgtaaagc agagtggtct tattttcatt cctaactatc
    22261 aattggatat taggaaagaa gaaaatcata aaaaatataa agtctatatt cgtgagaccg
    22321 cacaattctt catttacaac aaggaggcgt ctaaacttaa tcgctatatg cgagggcgtg
    22381 agctcattta ccagttaacc aatgatagca agagtattcc aaaaagaaga cgaccaaaac
    22441 cattgataca cttaagaaaa agattgaaga aataagtctc ctcattgagt tagatacgga
    22501 gaataagcct tatcaagata ttaaagatga cattgttaaa gatatggctc aattagattt
    22561 gaccattaca gagttgcagg accatattgc tcatctcaat aaggttgcgg aggtcttgct
    22621 taatatgaac aacaacgata tagagaaccg ccgtttggcc agatatgact atgccaagat
    22681 gaacttgact gcagctataa aaatagaaga agttgagaaa gagattgaaa cttctcaaaa
    22741 tgaacttaat atatccatag atgagtatga atatctagta agaaggttgg aaaagtttgg
    22801 agagatcttg agttatagca aaattatcga tacttctcga aatgaaatac aatgggagta
    22861 attatgatat aatttgggtg gtagcaattt ttatatcaga tattaaaatc tagttgatgt
    22921 ttcgtaaata actatattaa agaaatgatt cctgccgctt acattgagaa tagtttattt
    22981 gtaagtattt cattatttaa aaggggtaat aatgtataag attttagcca tagatgatga
    23041 taaagaaatt ctgaagctta tgaagactgc tttggagatt gaaaattacc atgttataac
    23101 ctgtcaagag atagaattac caatagtttt tgatgatttt aaaggatatg atttgatctt
    23161 attggatatc atgatgccta atataagtgg aactgagttt tgttataaaa ttcgggaaga
    23221 agttcattct ccaattattt ttgttagcgc tttagatggc gataatgaaa ttgtccaagc
    23281 tttaaatata gggggagacg attttattgt gaaaccattt agcttaaaac aattcgtagc
    23341 taaagttaac tctcatttga agagagaaga gagagcaaag ataaagaatg aggctgagga
    23401 gagagtgaag cgcagttttc cacctataga aatctatcta gaggaacgta tgttatatat
    23461 tgataaacag ccgttattcc tgacttatag agagtacgaa attttagaat tactgtcacg
    23521 tcatccgtat aaagttttca caaaagaaga gatatatgaa caagtatata gcgatgaagc
    23581 ttcagcattg tttcattcta tttcagaata tatctatcag attagaatga aattttcaag
    23641 ttttggaatt aatccaataa aaactattcg cgggattggt tataaatggg atgtttaaaa
    23701 aatattcaat aaaaaatcgt gtttggcgcg ccgtcgttga aattgtcttt ggagtttgta
    23761 tcactgtttt aatgattgcg ataatatcgc tttctttttc aaagttaaat atagtgacat
    23821 ctcataatgt gggagaagaa ttttatatta aagataaaca gtcaatcaaa cagttgaaca
    23881 attatatgaa gacattggga ttagattatg ttgtttttga tagaaaaaca gataaagcta
    23941 tggaaggaaa atatttatct aaagaatttt ctttatttaa cgaagttgca gaagaaaaaa
    24001 ataatctgac ttttaattct gtacattatg atttatatac taatatcaat tataatattg
    24061 ttataagata taatgagata ccggagtttt ctaaccatta ccttagaaat gtttcatata
    24121 acatgttgac attttatatt ctgggaatag gaacgagtat cagcattgta gttgctttaa
    24181 cacggtttgt aaaagaaatt tctttgaatt tcaaggaaat caagaagtta gcaaataaaa
    24241 tggggataga agtcctttct gaaaatgaaa attattctaa aataatcgag tttgatgata
    24301 tacttagaac tttacatatt aaaggtgata atttgaaaag tttgattgag cgagaaatat
    24361 tggaaaagca agatctatcc tttcaaattg cagcactttc gcatgatata aaaacacctt
    24421 taacggtttt gaaaggaaat cttgagttat tagaattgac tactttaaat aaaaatcagg
    24481 aaggttatat tgtatctatg aacaatagta tttctgtttt tgaaggctat ttcaattctt
    24541 taatatctta tacacgcatg ctttcagagg atagatcagt gaaactaatt ttagttgaga
    24601 agctattaag tgagttacat tttgaagtcg atgacttatt gaatatcaat aatattgaat
    24661 tttctatttg taatagactt attatcactt ctttttatgg agatgaggaa aatctgataa
    24721 gagctctttc taacctttta gttaatgcaa taagatttat gcccgtatta gataaaaaaa
    24781 tcgaagttat actgtctgag tcaggagagc aaattcattt tgaaatatgg aataatggtg
    24841 agcgtttcag tgatagtaca ctgaaaaagg gagataaatt attttacacg gaagattata
    24901 gcagaggtaa caaacattat ggaatcggtt tggcatttgt taaaggcgtt gctatcaaac
    24961 acggtgggaa tcttcaattg aataatccag ctagaggagg tgctagcgcc ataatctcga
    25021 taaaaaagaa aatttaatag atttgattga aactggagta tttcaggcaa aaaatcgtaa
    25081 atttttgtga tttttggcta aaatactttt tttctgaaca aattctgaac ttggggtgtc
    25141 ataataatca tgtaaatata tcggatgcaa aaacgtaaga gaggaactag aaaatgaata
    25201 acacaattaa agactttgat ctcgatttga aaacaaataa aaaagacact gctacacctt
    25261 atgttggtag ccgttaccta tgtacccctg gttcttgttg gaaattagtt tgctttacaa
    25321 caactgttaa ataataaagt tatcattatc atcaacatta taatttcaaa taatttgata
    25381 tatttataaa tttaggggag tagactgatt ttagtttgct cctttattag tagataagga
    25441 agttatacat gattaaagct cagctaaaga atctaataaa aacaattcat ctcgtaaaag
    25501 aggctggttt ctccctactg gtgttctcaa ttgctattag cttatttcaa gggattttac
    25561 ccatttttag tatgctacta gttcaaaaaa tgttaaacat tataacgact gatataaaaa
    25621 attttcacac attgatgatt gcttttatct catatattgc tttaacatta ttgacaatta
    25681 taataggaga agttgatagt tatattgata ccaaattaca gatacttctt cactataaga
    25741 tgaaccattt agttatgcag aaaactgtaa agttaacatt ggctgaattt gagacaccag
    25801 aaatctatga tgatatcact cgcatacaaa atcaaatatc ttataaacct tttcaaatat
    25861 ataagtcaat tatttctgta ttatcttcgt tagtatcatt aatttcctca tttgttattt
    25921 tattaaattg gaagatatca atttttccac caaggagttg aggttaggta cgttagaaag
    25981 ttattttcta aaaaaatatg ataaaaataa tcaattattt attaatcagg aaaatagtat
    26041 aaatagatct aaaatgaaat acggcatagt attaggtgta ttggagattg ctatacaatc
    26101 tattattatg ttccttgcag ttagagaagc atttctagga atattattag ttggaaatgt
    26161 aactactttt ataaggtctt tatctacaat gcaaacaagt acacaaaata tagtaaataa
    26221 tatctattct ttctataatg gttcattgta catggagtta ttgtatgatt ttacgcatga
    26281 tgagttagta cataacccta taatcgaaag tacggttgat acaattcagt cagttgaatt
    26341 tgaaagagtt tctttttcat ataatggtaa ggataatgta ataaacgata taagtttttc
    26401 aattaaagca ggagagaggt tagcaattgt tggtgaaaat ggttctggaa aaagtacgat
    26461 tttcaaatta gcctgcggtc tttacgataa ttatgaaggc aatatatata taaatggtat
    26521 taatcttcgt tcaatacaga aaaagtcata ctacaagaga atatccgcat tgttccaaga
    26581 ttttttaaag tatgaattga cactgcgtga aaatgttgga ttgggtgaat tatcaaagtt
    26641 atatagtgat gaggatttaa taagtgcact gaagtataca ggagttgatt cgatatttta
    26701 ctttaatcag gaaaaattaa atcagataga tctggaacag caattaggga attggtttga
    26761 ggatgggaga cagttatctg gtggacagtg gcaaaaaatt gcattatcaa gagtatatct
    26821 caaagaagcg gattgctatt tgctagatga gcctagttct gcgttagatc ctgagtctga
    26881 aacaaaaatt tttaaaacat tttttgaatt atcgcaaaat aaaattggta tttttattac
    26941 gcataagcca agcattactc aatctgtgaa taaaattttg tatctagata atggatgtat
    27001 agctgaaaca ggaagtttcg ctgatttaaa tattaaagga atgaagttta aaaacttatt
    27061 agataaagaa attggagcca catatgacac ctcaaattaa acagatagta gattacattg
    27121 cagatagttt tataaattat gaacagatag tagctgaaag tgagaataaa gtactaatta
    27181 atattgaatc taaatataga aatacctttt ttgataaatc tctgagtcat ggtgtgccat
    27241 cattaatctt aatgtatagt tcactatata aagtgacaaa agatgagaga tatctaacat
    27301 tctccaacat atacgttgag aaattagtag acataatctc tagagatggt atagaatctc
    27361 cgtctctcta tgctggcact gcaggtattg cattagctat taaagaagcg agtatatctg
    27421 gaaagtatta tccgaaatta ttatcatcct taaattgtct tctaaaagaa caaattaaag
    27481 acaaattggt agtaagtttt tctaatattg agaaaggaat tattgagcca tatgattatg
    27541 atatggttaa cggatttagt ggaataacta attacttact ccttgaacaa gaattttttt
    27601 ctgtagagtt aaatcaaatt ggaaattatc ttttgaaata tattgagaca attttaaata
    27661 aggtaactaa ttgttctgaa gatagagaag ctgaatttga tctaggaatt gcacatggaa
    27721 ttactggtcc tatgctaatc ctagcaaaat taaaatcaga aaaaattttg agtgtagaag
    27781 taggagatat actgaataaa gctataaatt taatgttttt atttagaagg gatgacaaac
    27841 tatggcctgg aaaaatttat actagtaata ttttggatct tgatttaaat tatcttccta
    27901 ctagaatggc atggtgttac ggtacgcctg gaactgcagt tgcccttttt aaaatgtgtc
    27961 aattgataga tttaaatttt attgacgata ttaatgaaag tttaattgct cagatgagaa
    28021 gttcagacaa aaatgtctat tctcttacaa tgtgccatgg tttggcagga gtaactttta
    28081 tatatgactt gattggtaaa aattattcaa gtgagaggct aactcgcttt gcagaagagc
    28141 ttcgccaaca aataattaca tcgttttcaa ctacaaaagt atttgggtat actgacagag
    28201 aaaaatttgg agaaatagaa gttgaactgg agtcaatagg gatattgcaa ggggtatcag
    28261 ggatagtgtt attcttatta aatccttaca gtgacgagga gctattgtgg caaagaatat
    28321 ttatttaatt tgaaaaggag aacaaaatgt ataaggtatt tccaactggt atgattcgta
    28381 ttccattgca aaaacaagaa ttttataata aagactatga agatttaata aatgataaag
    28441 tttttatgga acaactttta gttgctagtc ccagtcttta tgatagtgta atagattttg
    28501 ataaatgtaa tgaaaagaaa aagcaaaatg taaaagtctc attaaatagt tattctaaaa
    28561 gagcagcgtt tagaactact ccatttggta tctttactgc tattaataca gttgatttga
    28621 caaagggaac cacttctaat gtacaaaagg ttagttttat taaaaaagca gtcccagatt
    28681 attcatggat atattctcta gtaaaatcgt atgaaataag taaccttgaa aaactttcat
    28741 taaaaataaa tacagcggcg tttactcaag gtgacagata tgtactacca tttacagtga
    28801 atgaatcaga ggaagataga aacataagtt tttcaaaacc aattaagtta cttgtagaaa
    28861 aatgtaaaac tcgatatata aaatatgaag agttaataga tgtatttaaa aataattatc
    28921 cagatatagt gtcagacatg ctagagtcat atatacacga cttagttaca aatgattttt
    28981 taatttcaga tttaagacca ccaatttgta atatcaattc cttagattat ctactatcta
    29041 aattagaaga aggaacacta tgtactgact taactacttt aaagaagatg attgaagatt
    29101 acaacgattt aaatattggg gaaggtagag acttactttt gcagattttt gataaaatgg
    29161 aaagtattta taaatatgat aacggtggaa attacttaaa agttgattca ttagcagagt
    29221 atgaaggcgg ttttaatatt ctatctgaaa cggctcggga aagtatagaa cgactcgcag
    29281 agatgtttgt aaaattaaat acaaatgtaa agagaagagg ggggaggaaa aattcacttg
    29341 aagagtatca gttgaaattt attggcaaat atggagaaaa ctgctcaatt ccttttgttg
    29401 aagtaataaa taaagatgca ggcataggtt ttcctgaata ttacaaagga ggagaaggtg
    29461 aagcaataga gttaagcgat ccaataatgc aaatgtttga aaagaaatat gaggaagctc
    29521 tattaaatgg agggcatatc gaattttatt caaaagattt agatgatttt caaacagatc
    29581 aaagcaatag tttagattcg tttgaattaa attttaatat aaaaataata aataatgatg
    29641 tgaagttata tttaggagct aatatcggtt ctggacaagc aggtagaagc tttggaagat
    29701 tctatggatt aagcgaaaca gtaagagaaa caattaagaa tttaaaccat caaaataata
    29761 cgaatgtaga gttgtcattt gttcctaaac agataagact tgcgaatgta attcaaaatt
    29821 atagtgatga gtcttataat acttcatttt ttaccacatc atgggattca gagaacgaac
    29881 tgcgattgga ggatatttat ataagatata gtgatggtaa gtttcatttt acaactatcg
    29941 atgggaaaaa tgagttgaaa tttaccatga ataatatgct aaattccgat agccaaagca
    30001 gggttttgag attgttggtt gatttatctg agtttgagta cggtttatcg cattggtcat
    30061 tatttccttg ggatattttg gctcaggaaa gagtatatat tcctgaaatt ttatttgaag
    30121 acattacgat taccactgct cagtggaatt tatcagcaat tagagaagag attatacatc
    30181 aaaaaatatt tactggtaaa aaagatatat tcaataagtt tagaaagact tataagatac
    30241 ctaaagatat tattttgaaa tatgcagata atgaattgag gttagattta tctatagata
    30301 gtgatttaga gattttattt aaaaacatta ataaatatag gtttgtttct ttgagaggaa
    30361 ttgagcaggg agagtctatt tttaaggata atttagggaa ctacaataca gagatagtag
    30421 ttccaattct taaggaatct aatggaataa acaatacaaa cgataatgtt caatttaaaa
    30481 atgaagttaa gaaaatatat ttaccatttt gtgactggct atttttcaaa ttttatggtc
    30541 caaaagagcg acaagaagaa ttactaaata actggaaaaa attttggcta aacctgccat
    30601 cagaactgtt tagccaaagt aacatgtttt atatgagata taatgatacc aatgatcata
    30661 taagaattcg aataaattgt gattcgattg aaaataactt ttctttgtat ttgtcagtag
    30721 ttcaagatct attacctcaa ctgattgaga gttgtattat ctctgacata gaagtgagta
    30781 gctataaacc agaagtaaat agatatggag gacctcattt aataagttat gcagaggaaa
    30841 ttttttgtaa agaatctatc ttatttctta ataatacaat agaattttca gatgatgaaa
    30901 gattagtttg tgctacttac ttggttctat attatctaaa tcactttttc aaagataaag
    30961 aaacaaggcg ctctttctta tttgagaatt ataccggaaa gtataagaaa gatttcaaaa
    31021 atttgccaat tgatttacct attgagtata tgaagatttt gaatggagtc acctcagctc
    31081 ttgatagata tgattttttc caagaaatgg atagatatct catgtcttat atgaaagagt
    31141 ataatcgatt tggtggtaca aatgatatct ataatactaa attcaattta gtaggaagtt
    31201 ttttacatct atcaatgaat cgattaaacg ggattgatag agagtttgaa gagaaagtgt
    31261 attgttttgc ttactataca ctaaacgctc agcaatatat agaatgggag taactaatat
    31321 gttgaaaata caaaatctaa aaaaatctta tggtaagaga accatattga ataatgtcaa
    31381 tatgaatatt cctaagggaa aagtgtatgc tttaatcggt ccaaatggtg ctggaaaatc
    31441 aactattatg aaaattttga caggcttagt tagtaagaca agtggttcta ttatttttga
    31501 aggtagagaa tggtcacgtc gggatctgcg aaaaatcggg agtattattg aagaaccacc
    31561 actttataaa aatttgagtg cttacgataa tatgaaggta gttacaacaa tgcttggtgt
    31621 ttcagaaagc gctatacttc cattattaaa taaagttggt ctaggaaata ttgacaagag
    31681 accagtaaaa caattttctc ttggaatgaa gcaacggtta ggtatagcta tctctttaat
    31741 aaattcacct aaattactta tattagacga acctactaat ggcttggacc caattggaat
    31801 tcaagaatta agggaaatta tagagtcatt taaatcagaa ggaatgacaa ttatgatttc
    31861 aagccatata ctgtcagaag ttgaacatct agctgatttt attggattta tctatgaagg
    31921 aaagattgtt ctggaaaaag aatatgacgg ctctgagaat cttgaagagt tattcaataa
    31981 tcaaatttta tttgaaaaga ggaggtaggg gctatggtac aaattttttt atcaaattta
    32041 tttaaaataa aaaatacgag tattagcaag ttgattctta tttttcctat tatttgtgtg
    32101 atgttatctt tattgttttc agcattaggt ggtgaaagta tcctacgttt agctgcggaa
    32161 acaacagtaa atcagtgggg agttatttgg attaatgtta ttattgctgt aaattctggt
    32221 ttgttaaata aattagaatt agattctaat aagtattctg tttttttatc aagggatgtt
    32281 gatttaaaaa aggtagaata tgccagagtt ctattgatag ctcttataaa tctaataatt
    32341 agtatgattt taagcttaat gcttattgtg attagttttg tcctacctac tccaagtctt
    32401 attagtatag gtaggatact attaactatc ttgttaattt ggttgactac actatggcaa
    32461 atcccgttta ttttatggct atcaagaaaa attaatgtgt attttgctat gattattaat
    32521 attatatctc cactaattat tggtacaagt ttttctctct taaataaatg gtatttgttc
    32581 ccttatgatt ggtcgttgaa gttgcttgag ccaatgacaa gaatgagaat aaatagtata
    32641 ccttttggag cggagtttgt tccagactac tcacagattt ttatatcatt gttcctagga
    32701 attgcttttt tcatcttact gaccaatcta tttgctatct ctttcaaaaa acaggtgaaa
    32761 tagtcatgaa atcatattta tgttctaatt ttttaaaaat caaaaatact tcatatttac
    32821 taatacatat tttagctgca ctattatttc ccttacttct atttttctat tggagtcaac
    32881 gtggaggttt acaaagcagg actgtattat tttcctactt tcaaatagta ggtgtacttc
    32941 ttccttttgt tgcaagtatt gtgtgcattc aactaaaaaa tttggaagaa tcatctggaa
    33001 aatataaata tttattgggt tactcacagt cgaattataa gccatttatt gtagaattag
    33061 tatttctatg gttatgctat tgtatagtat taattatttc aattactata tttattcttt
    33121 tattgaaaac tattggtata aatgtatctc tcagactact tattttgaat agtttaattt
    33181 atatcatttt tgcctatgta acctatctga tcaatcatat tattagctat atatttagta
    33241 caggtgtggc attaggtatt tcaatggtag gtgttattgt tgcagctttt tgtgaaacga
    33301 gccttggtga taaggtatgg tttcttattc catgggcatg gcttttaaga atatcagata
    33361 ctctatataa ccaacagaaa atggcaattg ttccacttat tgttatattt tttgtttcat
    33421 gcacagtagc tttacttcat atacgaatat ttaaaagttg gaatcaagac tgtttgacaa
    33481 gtagttaaat acatctattt tgtttaccta acaataaata tgatttttaa gttataaata
    33541 ctatctaact tgtttaacta aaaaatgact ataaaatttc aatggtttac tagcttatat
    33601 ttaatagact tgtccaaata ggacaattaa acatttttta atcaaatctc ctttatactg
    33661 atattcggag aatgttagat gtatccgcgt attaggaatt tgagagaaga taacgatttc
    33721 actcaaaagt ttgttgcaaa tttactttcc ttctctcatg caaattatgc aaaaattgag
    33781 agaggagagg ttgccttaat ggcagatgtt cttgtgcagt tttctaaact ctataacgtc
    33841 agtattgatt atttattagg gcaaacagat tatccgtatc gttttcataa taaataaaag
    33901 tctcgccttg atttgttcat tgacaactga ataaaccgag gtgggacttt cttgtttgtc
    33961 gagcaattta gtgagatacg ccgtgatagg agcgataatt attagctata aaatagagtg
    34021 gttctctatc tgccatgacc acaaataggg acaatgatac ttctgaacgt tcaggctgcc
    34081 agcgtaaaag gccagtgggt gagattccca tgagtgattt aaccagtcat acccacggag
    34141 gttgtaattt ttttgaaagg agtggaggtg tgaaaatagt aacatataaa aagcttgatg
    34201 aatattggtt ttctcgaaca tactgcaaga aatattatta gagaagcaaa aagaatagct
    34261 ataaaaaagt tgaagaagct cgcgaacttg aacaaaatgc ggtacaatta agtaaatcgc
    34321 cctttgacaa tagaagactt ggtattgctc ctaaagatat tgtagaagag ttagtaggtg
    34381 ttctactctc tgaagaaagt ttagaaagtg agaattaaag atgactataa aaaatcaaaa
    34441 aaagtacaaa ggtgtttatt gtgataagaa tgggaagata ttttatcaag ctgatcttgg
    34501 tgtagatcca gtaacaggta aacgagttca aaagaaagcc agaaagaatt agtatggaaa
    34561 gccatttggt acaatgaaag aagcgtatga tgaactggtt cggattaggt atgagttcag
    34621 taataatctt agtattgaaa atgacaatat atcgtttgaa aactatatga atactattta
    34681 cctaagagca tataagcaaa aggttcaggc tgtaacttat gaaatagcct taccacatca
    34741 taagttattt attcagtatt ttggttctaa acttttaaag ggaataacag cttgagattg
    34801 tgaatctttt agtttacata ttattgaaaa ttatgcaaag aatttatggt cgcgattcaa
    34861 agcttgcatg gggtatgctg aaaggttaga ctatatttca aaagtgccat gtaaggcttt
    34921 ggataatcct aaagggaaac atcccgacac tccattttgg acttatattg agtttcagaa
    34981 ttttataaaa tcgtttgatt tgcaagatta tgaagagcta caaagattta caactatttg
    35041 gctatattat atgacaggtg ttcgtgtaag tgaaggttta tcattatgat gggaaaatat
    35101 cgatttcgaa caaaaattct tgaaagttca tacaaccttg gaaaaggatg aaaatggaaa
    35161 ttggtatcgt aaagaccaaa caaaaacgcc agcaggagaa cgtcttattg agttagatga
    35221 tgtgacaata gtagtattgg aaaattggag aagaaatcaa gtagtcaata ctgacacaga
    35281 tttcattatt tcacggtttg gtgaaccatt ttgtaaatca acgatttgtc gagttattaa
    35341 gcacaaagct caagaagtag gagtaccagt gataacggga aaaggcttgc gacatagtca
    35401 tgcttcctat cttattaatg ttttgaaaaa agacattctg tacgttgcaa agtgtatggg
    35461 acatgctgat aagtctacaa ctttgaatac atatagtcac tgatttaacg cattagataa
    35521 aacagtatct gaagaaataa cccaaaatgt aataagttca ggattagatt ccattttata
    35581 ccaaaattcc tgccaaacaa ctaattgata taacgaagct tattatatcg cccatataac
    35641 aggatgtgtt attgttacag ggtacttgct ttatgagctt gtttaagcta ctaaaggtgg
    35701 gtgccactat acttccttcc aaatttctga gaatttttgc tataatagta actatgaaag
    35761 atataatgat aaaaaggtgg gttatccttt tttttatggt attaacttgt tcagggtttg
    35821 gtaaaacagt tctcgcagct gattttcaag tgggagcaga acatgcgatt gtcgtagagg
    35881 cagactctgg gagagttctg tatgagaaag acgctaagac gccagatgct attgcctctt
    35941 tgaccaaatt ggtgacggct tatctggttt tagataaggt taaatcaggc cagctgcaat
    36001 tatcagatca agttaatctc tcagattatg cctttgagtt aacaaaagat aggtctttaa
    36061 gtaatgtacc ttttgacaaa aaaacttatt cagttcaaga cctgttgaca gctactttgg
    36121 tagcaagctc aaatagcgct gctattgcgt tagccgaaaa agtggcgggc tcagagcttc
    36181 attttgtaaa tcaaatgaga gaacagctat ctcattgggg gattacttct ggtaaaatcc
    36241 tcaacgcctc aggtctgcca aatgaggtgt taaaggacaa tcgttatcct ggttctgcgt
    36301 tggaagaaga aaataccttg agtgctcaag atgttgcagt tgtgacgatg catttgttag
    36361 aagattttcc tgagatttta gagatcacca aacaaacaga agttaatttt gctggcaatt
    36421 ctattaaaag ttttaatcaa ctcttaccag gtatggctaa gggtagagca ggagtggatg
    36481 gtttaaagac tgggacaaca gacttggcag gtcattgtct tgtggtaact tctattgaaa
    36541 atggtatgcg agtgattacc gttattctaa atgccgatgg atctgataaa aaccaaaaca
    36601 cgcgatttga gcaagcaaat cgtttgttgg actatgtgga tagaacttat tgtcgtcgta
    36661 agattcttaa aaaaggaagt cttgtcttgg agcgatcgct ccctattcaa gatggccaag
    36721 taaaagaact accggtatct gttgccgaag atgtaaccat cattttgcaa cagggtgaac
    36781 aagtccccaa acctaaacaa tttatgatat cagagacttc tttactagca ccgattacta
    36841 aaggtgaagt ggttgcttat ttgacgtctc cacgaataac agatcagtca gtgaggtatt
    36901 taaaggaacc taagcgtatt cctcttaaag ctagccaatc tcttaaaaaa gcctctgact
    36961 tacagctgtg gtggcgagat tttcttgaaa aaagacgtta aagataagaa taaaataatt
    37021 ttaaaacaat caaaaaagac agttatgaaa gaattttttc ataactgtct ttttagttta
    37081 cagtgagtat gatttggaat gataaaaaaa ttgatgttat ggttccattg tttgtaaaag
    37141 attttccgga ctcatgtttg ggaggatacg aatccggtta agagaaagaa gtccattagc
    37201 cgcgctagct attccttcgt tagtggtaac acctaattta tagttaagtc tagctgcaat
    37261 ttgtaaggta gtgtcattat atctaccaga tggataagca atggcaatag tgttttggtt
    37321 taattgctta tcaagatagt cctttgaatc tttcatttca gttgtttgaa catcaggact
    37381 agcctgctct aaatcaggat gattcactgt atgatcttgg aaagacatac cgaattgttt
    37441 catttctttc atctgcttaa gagtcagatt ggcagcgctt cctatttcgg ttagtcctgt
    37501 aataacatta ttggtagctt tagcatcata tttttttaag attgggtaag caacgttgta
    37561 gaaatcaatc atactgtcat caaatgttag ccatacaact tttttagcag gcaattcatt
    37621 atttgaaaga gcgcggtaga cctcttcagg actcaaaaaa tagtagccct cgtctttcat
    37681 tttttgaagt tgttgatcaa agagatctgg gttgacaatc aaattagcat tggccgtctc
    37741 ttcagggaac attacatgaa tagcatgata cattaaaatg gggatcttga caggtgtttc
    37801 ttgcttggtc caagttaatt tagaatgggc ctttttttga gattttgagg aatcgaaatg
    37861 agtcttctta agttttgtcg tttctttttt tgccgtcttt ttgtgatgaa aaagacttat
    37921 tttaggtaca aattgagaga catctgacaa gccgaatttg ttagaccctt ggtataaata
    37981 gaaaatgagg gtcaaaccaa gaatgcaggc aaataatagt ataaggttaa tgagagtgaa
    38041 ggcttttctt ttttggcgcc gtttatgtct attgttcatt acttgctcct ttttttgata
    38101 taagtacctc tattgtaaca ttttttagag aaaagaaggc ttgaagtcag ccttttttct
    38161 tgttttttgg aagaacgtgt attaaggagt aaaaaaattg ttgtcacaga taacacttta
    38221 ggaggtagca aagactatgt ggggctggta ttcttatcac gtaaaaaaag caatgaagat
    38281 aaaagtgtag tgtactttag aagttatttg agtgtttggt gaggataata aaaaaaaagg
    38341 gtactactct aggttatctg ctgtcacttt tttttatata tggtatacta aagatagatg
    38401 tgaggtatga aatgacttat gaagagactc tagaatggat tcatgatcac ttagttttcg
    38461 gcatcaagcc aggcttaaaa cgtatgcttt gggttcttgg acagttagga aatccccaaa
    38521 aaaatgtcaa gggagtgcat atcgttggaa ctaacggaaa aggttccacg gtcaatcatt
    38581 tacaacacat ctttacgaca gctggttatg aagtggggac ctttacctct ccttatatta
    38641 tggatttcaa agaacgtatt agcataaatg gtcgaatgat ttcagaaaaa gatttggtta
    38701 ttgctgctaa tcgtattcgt cctttgacag agcgtctagt tcaagagact gattttggag
    38761 aggtaactga atttgaagtg attactttga tcatgttcct ctattttgga gacatgcatc
    38821 ctgtagatat tgctattatt gaagcaggcc ttggcggtct ttatgattcg accaatgttt
    38881 ttcaagcaat ggttgtggtc tgtccctcaa ttggtttaga tcaccaagct attttgggag
    38941 agacttacgc tgatattgcc gcccaaaaag ctggtgtttt agaagggggc gagacccttg
    39001 tttttgccgt tgaaaacccg tcagctcgag aggttttttt aacaaaagct gagcaggttg
    39061 gtgcttcaat ctgggaatgg caagagcagt ttcaaatggc tgagaacgca tctgactatc
    39121 gttttactag tcctttagga gttatttcag atattcacat cgctatgcca ggacaccacc
    39181 aagtgtctaa tgcagcttta gctattatga cttgcttaac cttgaaagac cgctatccta
    39241 ggctaacttc agatcatata agggaaggtc tggcaaatag tttgtggcta ggccgtaccg
    39301 aattattagc accaaatcta atgattgatg gggctcataa caatgaaagc gttgctgctt
    39361 tggtagccgt cttaaaaaat aactacaatg ataaaaagct tcatattttg tttggtgcga
    39421 tcgacaccaa gcctattgcg gatatgttgg ttgctcttga gcaaataggt gacttgcagg
    39481 ttactggctt tcattaccct aacgcctatc cattggaaaa atacccagaa cgttttggta
    39541 gggttgctga tttcaaagat ttcttggccc tgcgtaagca tgccaaagca gatgactttt
    39601 tcgtgattac agggtcgcta tattttattt cagaaattag acagtattgg aaaaaacata
    39661 ttgaaaaaaa cgttctttta acccattaaa aaagagaaaa tgaaaaggaa aaggaaaaaa
    39721 ctaatgaaaa gggaaagact aatgtctatt aataaggaaa aagcagaagc agccatttat
    39781 cagtttttag aggctattgg tgaaaatcca aatcgggaag gtcttcttga cacgcctaaa
    39841 cgggttgcta aaatgtatgc ggagatgttt ttgggactgg gaaaagatcc taaagaggag
    39901 tttacggctg tctttaaaga acaacatgag gatgtggtga ttgtcaaaga cattagtttt
    39961 tattctatat gtgaacacca cttagttcct ttttatggca aggctcatat cgcttacttg
    40021 ccaagtgatg gtcgagtaac aggtttgagt aaattagcgc gagcagttga agtagctagt
    40081 aaacgacctc aactccaaga gcgtttgacc tctcaaatag cggatgcttt agttgaagcc
    40141 ctcaatccta aagggacttt agttatggta gaagcagagc acatgtgcat gaccatgcga
    40201 ggcatcaaaa agccaggtag taaaaccatt accaccactg ccagaggctt atataaggaa
    40261 agtcgtgctg agaggcaaga agttatttct ctaatgacaa aagattagga gaacttatga
    40321 aaattggaaa gtttgtgatt gagggcaatg cggctatcat ggggatttta aatgtgactc
    40381 cagattcttt ttcagatggg gggtcttaca ctactgtgca aaaagcatta gatcacgttg
    40441 agcaaatgat tgctgatggg gctaaaatca tcgacgttgg tggagaatca acacgtccag
    40501 gttgccaatt tgtaagcgct accgatgaaa ttgaccgggt ggttcctgtg atcaaggcca
    40561 tcaaagaaaa ctatgatatt ctaatcagca ttgataccta taaaaccgaa acagctagag
    40621 cagctttaga ggcgggtgcc gatattctca atgatgtttg ggcaggtttg tacgacggtc
    40681 aaatgtttgc cttagcagcc gagtacgatg cgcctatcat cttgatgcat aaccaagacg
    40741 aagaagttta tcaagaggta acacaagacg tttgtgattt tctaggcaat agagcacaag
    40801 cagctcttga tgctggcgtg ccaaaaaaca atatttgggt tgatccagga tttggatttg
    40861 ccaaatctgt tcaacagaat acggagttat taaaaggatt ggaccgcgtc tgtcatttgg
    40921 gctatcctgt cttgtttggt atttcgagaa agcgtgtcgt agatgccttg ttaggcggaa
    40981 acaccaaagc taaagagcga gacggagcga cagcagcctt gtctgcttat gcccttggaa
    41041 aaggctgtca gattgtacgc gtacacgatg tcaaggctaa tcaagacatt gtggctgtgt
    41101 tgagccagtt gatgtgagga cttatggata aaatcgtatt agaaggttgc cgtttttatg
    41161 gctaccatgg agcctttaaa gaagaacaga cccttgggca aatttttctg gttgatttag
    41221 aattgtcagt cgatttgcaa gcagcttctt tgtcagacca attaacagat acggtccatt
    41281 acgggatggt gtttgatagt gtccgtcagt tagtggaagg ggaaaagttt attttgattg
    41341 aacgtttagc aggtgcgatt tgtgaacagc tttttaacga atttccgcct attgaagcca
    41401 tcaaagtggc cattaaaaag gaaaacccac cgattgcagg ccactataag gcagttggta
    41461 ttgaattgga gagacagaga tgaccattgt ttatttaagt ttaggcacca atatggggga
    41521 ccgagcagct tacttgcaaa aagctcttga ggctttagct gacttacctc aaacacggtt
    41581 gcttgctcaa tcatccattt atgaaacgac tgcttggggc aagacaggcc aggctgattt
    41641 tctcaatatg gcctgtcaat tagatacaca attaaccgcg gctgatttct taaaagaaac
    41701 acaagctatt gagcaatctc ttggtcgtgt gaggcatgaa aaatggggat caagaactat
    41761 tgacattgat atcttgctat ttggagaaga gttttatgac acaaaggaat tgaaagtgcc
    41821 acacccttat atgactgagc gtgcttttgt tttaatcccc ttattggaat tgcagccaga
    41881 tttaaaattg cctcctaatc ataagttttt aagggattat cttgcagctt tagatcaatc
    41941 agatatcacg ctcttttcag ctcaacagac agagttctag actctgtttt cggagtaatg
    42001 cctgctataa gccaatccat aaagttttgc caaattcatc gttatttttt ctaaaatttg
    42061 atataatagt atcggcttta tgccgatttt tttacgcgta taagaagtga taaaagaaag
    42121 aagataacta tgataactga acttcatggg attgatattc gagagaatga acccctaaaa
    42181 cattacactt acactaaggt aggtggccca gcagatttct tggcttttcc tagaaatcac
    42241 tacgagctat cacgcatcgt tgcttatgcc aacaaagaaa atatgccttg gcttgttttg
    42301 ggaaatgcca gcaatctcat tgtgcgagat ggtggtattc gtggctttgt catcatgttt
    42361 gataagctaa atgcagtgca cttaaatggt tataccttag aagccgaagc tggtgccaat
    42421 ctgattgaaa caacgaagat tgccaaattc catagtttaa cagggtttga atttgcatgt
    42481 ggcattcctg gaagcattgg gggtgctgtt tttatgaatg cgggtgccta tggtggggaa
    42541 atatcacata ttttcttatc cgctaaggtg cttacctcaa gtggagagat caaaaccatt
    42601 tcagctaggg atatggcttt tggttaccgt cactctgcca ttcaagaaac aggtgacatt
    42661 gtcatttctg ctaagtttgc tcttaaaccg ggtaattatg atacgatcag ccaagaaatg
    42721 aatcggttaa atcaccttcg ccaactaaaa caacctttag aatttccatc ttgtggatct
    42781 gtgtttaagc gcccgccagg acactttgca ggtcaattaa tcatggaagc aaatcttaaa
    42841 gggcatcgga ttggtggtgt ggaagtttcc gaaaaacacg ctggttttat gattaatgtg
    42901 g
//
LOCUS       DAUVUY010000006        35556 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.006, whole genome shotgun sequence.
ACCESSION   DAUVUY010000006 DAUVUY010000000
VERSION     DAUVUY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35556
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.006"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            3..560
                     /locus_tag="VOX58_000309"
     CDS             3..560
                     /locus_tag="VOX58_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HES5877578.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLETKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            complement(596..1420)
                     /locus_tag="VOX58_000310"
     CDS             complement(596..1420)
                     /locus_tag="VOX58_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HES5877579.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(1422..2039)
                     /locus_tag="VOX58_000311"
     CDS             complement(1422..2039)
                     /locus_tag="VOX58_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HES5877580.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAVVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            complement(2236..3213)
                     /gene="lacD"
                     /locus_tag="VOX58_000312"
     CDS             complement(2236..3213)
                     /gene="lacD"
                     /locus_tag="VOX58_000312"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HES5877581.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            complement(3260..3713)
                     /locus_tag="VOX58_000313"
                     /pseudo
     CDS             complement(3260..3713)
                     /locus_tag="VOX58_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            complement(3723..4238)
                     /gene="lacB"
                     /locus_tag="VOX58_000314"
     CDS             complement(3723..4238)
                     /gene="lacB"
                     /locus_tag="VOX58_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HES5877582.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVNGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLESHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            complement(4253..4678)
                     /gene="lacA"
                     /locus_tag="VOX58_000315"
     CDS             complement(4253..4678)
                     /gene="lacA"
                     /locus_tag="VOX58_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HES5877583.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            complement(4935..6386)
                     /locus_tag="VOX58_000316"
     CDS             complement(4935..6386)
                     /locus_tag="VOX58_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HES5877584.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYADHNHLPVLVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            complement(6412..6717)
                     /locus_tag="VOX58_000317"
     CDS             complement(6412..6717)
                     /locus_tag="VOX58_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HES5877585.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQMSDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEMPIPTVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            complement(6710..7183)
                     /locus_tag="VOX58_000318"
     CDS             complement(6710..7183)
                     /locus_tag="VOX58_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HES5877586.1"
                     /translation="MFPNILFTEARTQPELFDLVASYLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            7420..8190
                     /locus_tag="VOX58_000319"
     CDS             7420..8190
                     /locus_tag="VOX58_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HES5877587.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQFTQLLVAHENKE"
     gene            complement(8394..8597)
                     /locus_tag="VOX58_000320"
     CDS             complement(8394..8597)
                     /locus_tag="VOX58_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HES5877588.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGQAKVT
                     GRPLTFLLKRALKDTKFELHQSS"
     gene            complement(8611..10842)
                     /locus_tag="VOX58_000321"
     CDS             complement(8611..10842)
                     /locus_tag="VOX58_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HES5877589.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKMVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPPFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGYP
                     NMDSLVALATSFAFAYSLYGVVEISLGQANFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTSQAIQKLLSLKATVVRVWRNSQWQLLPLEEVSYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGPGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADKVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQKLLT
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNAALMGILGIDLSCVQSVCAKASEKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALHQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVIADLRSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            complement(10842..11279)
                     /locus_tag="VOX58_000322"
     CDS             complement(10842..11279)
                     /locus_tag="VOX58_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HES5877590.1"
                     /translation="MNQNISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKT
                     LIGRLVEKKALGTYRQGRAYIYQALLDETLLQKEALATVLDGICQRQHTHLLLERLYD
                     LPMTLEEIEAFQELLEVKKENAVLEVSCNCLPGQCHCQKKETY"
     gene            11442..12443
                     /locus_tag="VOX58_000323"
     CDS             11442..12443
                     /locus_tag="VOX58_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HES5877591.1"
                     /translation="MKKTLKSTLILLGLTLAIGASTQVDASSRSWKSWFIEKYFWLKG
                     DKGYYSEQDETKFDQYVNSAGEASNKGFDLKEEQVNGTLVTKTVDNMQVHTWNENPSN
                     KANQKVIFYLHGGSYLNNPTTYHIDMLKTLSTSLDAKIILPIYPKAPTYSYSDAMPKL
                     VDLYRDTLKETTAQNITIMGDSAGGGLALGLALALKNESLKQPKDIVLLSPWLDVTMS
                     NPDIESYESNDPILSAWGLRKVGEIWARRDPNNTKHPYVSPINGDVSGLAPITMFVGT
                     HEIFYPDVKKFDDILTAKGHQHELIVADKMNHVYPIYPIEEAKTAQYQIIDTIQKAY"
     gene            complement(12496..12846)
                     /gene="rbfA"
                     /locus_tag="VOX58_000324"
     CDS             complement(12496..12846)
                     /gene="rbfA"
                     /locus_tag="VOX58_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HES5877592.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDKKD"
     gene            complement(13052..15913)
                     /gene="infB"
                     /locus_tag="VOX58_000325"
     CDS             complement(13052..15913)
                     /gene="infB"
                     /locus_tag="VOX58_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HES5877593.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            complement(15933..16235)
                     /locus_tag="VOX58_000326"
     CDS             complement(15933..16235)
                     /locus_tag="VOX58_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HES5877594.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAVADAGFSKKMRTLME"
     gene            complement(16228..16524)
                     /locus_tag="VOX58_000327"
     CDS             complement(16228..16524)
                     /locus_tag="VOX58_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HES5877595.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            complement(16540..17697)
                     /gene="nusA"
                     /locus_tag="VOX58_000328"
     CDS             complement(16540..17697)
                     /gene="nusA"
                     /locus_tag="VOX58_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HES5877596.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV"
     gene            complement(17872..18408)
                     /gene="rimP"
                     /locus_tag="VOX58_000329"
     CDS             complement(17872..18408)
                     /gene="rimP"
                     /locus_tag="VOX58_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HES5877597.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            complement(18516..18602)
                     /locus_tag="VOX58_000330"
     tRNA            complement(18516..18602)
                     /locus_tag="VOX58_000330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18566..18568),aa:Ser,seq:gga)
     gene            complement(18667..19302)
                     /gene="trmB"
                     /locus_tag="VOX58_000331"
     CDS             complement(18667..19302)
                     /gene="trmB"
                     /locus_tag="VOX58_000331"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HES5877598.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            complement(19302..20093)
                     /locus_tag="VOX58_000332"
     CDS             complement(19302..20093)
                     /locus_tag="VOX58_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HES5877599.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            complement(20157..21191)
                     /locus_tag="VOX58_000333"
     CDS             complement(20157..21191)
                     /locus_tag="VOX58_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5877600.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGYAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAILLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNVLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            complement(21194..21919)
                     /locus_tag="VOX58_000334"
     CDS             complement(21194..21919)
                     /locus_tag="VOX58_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5877601.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            21991..22410
                     /locus_tag="VOX58_000335"
     CDS             21991..22410
                     /locus_tag="VOX58_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HES5877602.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQLATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEKDGISIQYTTHEPNFPVLEKLARQINQEVSS"
     gene            22407..22763
                     /locus_tag="VOX58_000336"
     CDS             22407..22763
                     /locus_tag="VOX58_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HES5877603.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQEDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(22874..24157)
                     /locus_tag="VOX58_000337"
     CDS             complement(22874..24157)
                     /locus_tag="VOX58_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HES5877604.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDSTASSH
                     TPTTPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(24166..24690)
                     /locus_tag="VOX58_000338"
     CDS             complement(24166..24690)
                     /locus_tag="VOX58_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HES5877605.1"
                     /translation="MIQQSLVIEEGVPQDAKAVLSLVAAAAKETDFITGVETILEATP
                     HELSDFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQVEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            complement(24665..25126)
                     /gene="tsaE"
                     /locus_tag="VOX58_000339"
     CDS             complement(24665..25126)
                     /gene="tsaE"
                     /locus_tag="VOX58_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HES5877606.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHDSAKSRY"
     gene            complement(25280..26740)
                     /locus_tag="VOX58_000340"
     CDS             complement(25280..26740)
                     /locus_tag="VOX58_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HES5877607.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSSVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            27004..27816
                     /locus_tag="VOX58_000341"
     CDS             27004..27816
                     /locus_tag="VOX58_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HES5877608.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKIIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTHHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
     gene            28168..29160
                     /gene="manX"
                     /locus_tag="VOX58_000342"
     CDS             28168..29160
                     /gene="manX"
                     /locus_tag="VOX58_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HES5877609.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            29249..30058
                     /locus_tag="VOX58_000343"
     CDS             29249..30058
                     /locus_tag="VOX58_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HES5877610.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAATEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            30075..30986
                     /locus_tag="VOX58_000344"
     CDS             30075..30986
                     /locus_tag="VOX58_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HES5877611.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            31100..31459
                     /locus_tag="VOX58_000345"
     CDS             31100..31459
                     /locus_tag="VOX58_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HES5877612.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGIGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            31851..33128
                     /gene="serS"
                     /locus_tag="VOX58_000346"
     CDS             31851..33128
                     /gene="serS"
                     /locus_tag="VOX58_000346"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HES5877613.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRSYMGGETVISPK"
     gene            complement(33361..34131)
                     /locus_tag="VOX58_000347"
     CDS             complement(33361..34131)
                     /locus_tag="VOX58_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HES5877614.1"
                     /translation="MTDVSRILKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(34128..34994)
                     /locus_tag="VOX58_000348"
     CDS             complement(34128..34994)
                     /locus_tag="VOX58_000348"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HES5877615.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            complement(35003..>35556)
                     /locus_tag="VOX58_000349"
     CDS             complement(35003..>35556)
                     /locus_tag="VOX58_000349"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HES5877616.1"
                     /translation="GTIEFLLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIK
                     IAAGQPLAISQEDITITGHAIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSA
                     VYNGYAIPPYYDSMIAKIIVHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDK
                     RVIAGDYDTSFLMETFLPHYNKD"
BASE COUNT        10865 a         7651 c         6249 g        10791 t
ORIGIN      
        1 tgatgactga taaagcactc tcaaaaagat cacttcaaaa cttaacacaa tttaataaag
       61 aaacacgacg catagcacgt gaatctatgg aaatcgcctt actgaatctt ctagaaacaa
      121 aacctttagg tgatattacg atttcagaat tggtgacgaa agcaggagta tctcgtaacg
      181 ctttttatcg taattatacc tctaaagaag ccattataga acaactgtta gtaggggtta
      241 tccgtcgtat ttttcgtgga ctaaaacaat ttgatattaa aactcaggct tatcaagcct
      301 ggctctatct ttttactgaa gctaaaaaag aagctcagct tcttaaaatg attttcaaac
      361 atcacctgca ccatcttcta acacgattag tcactaaacg tctgaaagct tatcaaaaat
      421 taaaagacaa aaaacaatcg cactataagc gccttttttg gagcaatgct attgtttctg
      481 tcttgacaaa ctggatttca gatgacatga tcgttcctgc cgaagaaatg gctgctatgg
      541 gcttgcctct attaacgtaa aaaggttcct tttgggaacc ttttttatat aatagttagt
      601 ctttttgagc tgcaagtgca aactgacgga tagcctgggc cacacccgcc atatcattag
      661 tcaaagtgac tttgtcagct aatggcttga tagctgctga agcattttcc atagcaactc
      721 ctaaaccagc ataagttaac atctcaatgt cattgggagc atccccaata gccatcactt
      781 ggtcagcagt taaacccaaa tcttctacta attctttgag agccgatgcc ttgctgactc
      841 cctgagggag aatttctaaa atattatctt ggctacgaac aacatgaaaa ctctgactaa
      901 gctgatttct caccgcacgt tcaaaggcat ctagcgcagc tttttctcct aagtacatgg
      961 cttgaaaaat caggcgagga gtgtccgaca gctctgctaa ggagacaggc ttgacaatgg
     1021 taaataccaa atctccatct tcctgcacaa ggtctggcac ttcttcttca agcaccaaat
     1081 aatcgttttc ctcagttaaa gtcaggtaaa tatctggaaa actttggctt agctcttcca
     1141 gcaattcaat atctgaaacc ttcaacattt ttgaatggcg taattgccag tcaggactac
     1201 tataagtact acagccatta ttgataatga ggaattcctc ttgtgtcaaa cctaattggt
     1261 caaaataggg tcttgtccct gactgaggcc ggcctgtgca taagacaatt tttaagcctg
     1321 actgagctgc ttcttggatg gctgtgatat tctcttttgg aatctgtttg tcctggttta
     1381 ataaggtgcc gtccaaatca attgcaatta gttgaatcat cctatctttc ctctttccat
     1441 ttgattttag ggaggttcca ttttttacgg ttgccaatca tgcgtaaagt cgtgagtaaa
     1501 acaaccaagg cataacattc aatcgttgaa tctgctagtt taaaatgcaa aacaatagct
     1561 gcaagaatag accacccagc atagatttca ctacgcaaaa cactaggttt tcgccctgcc
     1621 agaatatctc tgacaacacc accgcctgca ccagtcaaca cagctgctac aatgacagca
     1681 cttaaaggct ggttaagacg gacagcatgc aaggctcctt ggacactaaa agctgccaga
     1741 ccaatggcat cagtcaaaac aactgcccgt acccaacctc tagctattag cttaggaaaa
     1801 agcataatca aaaccatagc aaagaaagcg caagtgaatt ctggcttttg cccccataag
     1861 gcctctatag gtaaaccaat tagggtgttt cgaatggctc cgcctccaaa ggctgtcacg
     1921 aaacctaaaa taaaaatccc taaaatatca aattcttctt ccatggcaac aatagcacca
     1981 gaaagagcaa aggcaatggt cccgatgata tttaaaattt cccacatatc aattgtcata
     2041 agctacccta ttgtctttta gtcttctttt agtttaccac gttttccttt aaaaaataag
     2101 tagaaaaaga taaaatgtaa ggataaaaaa gcaaggataa aacccatttt catccttgct
     2161 acaaaagatt caaacaatta tccccatact tatcttatca catcaatgag agctgcttga
     2221 taggataagg ttgttttaga ttttttccgt ccaagggcta gctgtctctt cgagtaccct
     2281 gtttaactcg tcaatatttt ggaagccttc agtgcatagc cactttcttg cttcgtcctc
     2341 tcctttagta atataaactg gaacggatcc tgcccacgtg gcacgcccac acaaaacacc
     2401 gctaaattga gcacctgctg ctgctgcaaa gtataatgtc tcttgaaaaa gtttggcaga
     2461 tacgcctgca ctcaaataga tataagggag gtggctggca gcttcttggt cttgaaaagc
     2521 tttaatagcc tcagcttgac tgtaatggac ttccccttca gtaaaacctt ctacataagc
     2581 catattaaca ggaacctcta cttttagcac atcaacgcca aaacgcttat ccgaaaatac
     2641 tgacatagca ccgttaacct tatgtggctt aagctttgcg tatgccgcac tgttgttatc
     2701 tgatattctc tcatcataac tcaaaagttc aagaaaaaaa gggatgtctt cagctgtaca
     2761 ttctgaacca atgcgttcaa tataagcttg tttttgtaaa ttgatttgtt catctccatc
     2821 aacatcataa tagagcaaaa atttaatagc atctgcacct gctgctttca accgtttgac
     2881 cgaccattcc actaagcaat cgggtaaacg gctagtggtt gtagcatcat aacctgtttt
     2941 ttcgtaagct aacagtaagc ctgtctgatt agctcttacc ttagtagcca gcaacccata
     3001 ttctggatct aataaaatag atgaagcata aggcgtcaat tcctcagaaa cgagaacttt
     3061 caatgtttct atctgtgtaa ctgtcgcttc tccttcttga tgtgctgcca tcatttgctt
     3121 cagtgcacct cgttgatcaa aagctaaagc tgatatgatt ccttgatggc tcactttttc
     3181 taagtaatgt cttttatttg ctgtgattgt catgtgatac ctctcttact tgaagttgct
     3241 gatataattg tttaaaagtt gccatagtca tcaaatcttg ataatagtga gtagctagtc
     3301 tcctaggcag actgcctgag aagttaagac atcaaacggc ttagcaatag tactcatgtg
     3361 gctcaaagag acttcggctt cgtccacatc actgtaggga ccagcttcca aaatttttgt
     3421 ctgcatgcct tcatgtaaaa cagcaataca atttctcgtt tcccctttga ttttgaaaaa
     3481 ttggttagaa atgccctgtt cttgtaactg atgaatgata aagtcaccca gtttaccacc
     3541 actaaatcct gttgccataa ctgaccagct tttgctaaca ttctggtcac atttagctcc
     3601 ttatcaccag ctgtttttgc cgttctgtca acacgattaa ccgtgtccat agccagacaa
     3661 tctaaaggat aggaaatagc gacagaagga tttaagataa ctgttaaact catctcttct
     3721 tcctaatcgt gatactcacc acgatcccat ttttccaaaa attctgtaaa aaaatcagga
     3781 tcttcttggt tggcatgatg actttccaag tgggctattt tagcaattag cttcttattc
     3841 tcttcagttt ctttgtattc tgccttgata aaagcatcta taatatcaca cataagaagt
     3901 tcaccagtga ttttaccacc aaagccaatc acattggcat tcaattcttc cttggcataa
     3961 agcgctgttg tcatatctcg aacaagggct gaacgaatac ctggtacctt gttcacggca
     4021 ttattaatgc caactccagt tccacaaata caaacaccca agtctgcttg accattaacc
     4081 acagcttcgc cgactttttt tccaaaaata gggtaatggg ttcttgtatg gtcataagtg
     4141 ccacagtcaa taacgtcata gcctttagat ttcaaaaaat cagaaacagc cattttttcg
     4201 ttggtgacaa tatggtcaca accaatcgca attttcatca ctttatcctc ctttaggcca
     4261 ttttattgag catgtcaaca cgaatttgat gacgaccacc atcgtaggga ccagtcacaa
     4321 aacctttgac gatattcttc gctaattctg gacctacaat ctctgcacct attgtgatca
     4381 tgcgggcatt gttgtgaccg cgcgtcatgt aagcagagcg ttcatctgac acttcagcag
     4441 caaccattcc ttttaatttt gtggctacca taaagggacc tgctccgtaa gcatctacca
     4501 taatgcctaa acgaccttct gcttcattaa cggctttcgc caccgctaat gtgttatcaa
     4561 taaaatcact gttaatatca gtcacgtcaa taatatcata gccttcttct tgaagaaaac
     4621 ttttgatcaa ttcttttaat gcatttccgt gtgcatctgc acctaaaata atagccatag
     4681 cttttccttt tctaacccaa aacgcttgcc aatctagaga agggggacat gattacctgt
     4741 ccttagatca agatgattgc cagtagtctt agtgtcttcc attatttctt tcaagacaaa
     4801 gcacttgtga gttcccacag agcgccttgc cttacatgag aaatactctt ttcacgttag
     4861 ggggtaaggc agaaatacct tacgcagtta tctgctattc tccctaattt tttagtcagt
     4921 cacctgacct gattttattt cattgttttc gcatactcag cgttacgtgc cttcatttgg
     4981 ttagcatacc aagcaaagag acctaggtaa atggccacaa agatgattag cgtgatgatt
     5041 gaggcaatct gacctgttgt cgcttgacca atcacataac caaaaatttt ttccattggt
     5101 ccttcaagtg ttgaatgcgt aatcaaggaa ccagcgcttg tacctgctgg aaaagcccct
     5161 acttttttag ccaactctgt cgcaaatgga gcaatcatag ttcctgacaa gaggaataat
     5221 ggcaataaga gggaaccaaa aataatcatg cgaatcaaac gaccacgtgt gaccacaaga
     5281 agagctggtg tcatccccat agcaataatc cccgccaatg gtaaaatccc attgccaact
     5341 tttgatagga gaacggcttc aatcaacatg attggggcaa gaatattggc acaggcccag
     5401 atttcagcgc gaccagcaat gaaaggccag tccaaaccga tattgaaacg acgcccatgc
     5461 attctagcat tggcaaaatt ggtaatccct tgtgacaaag gctcaacagc tgcgataaac
     5521 caagagccaa tcaaagagaa aagttccaag caggcaccag ctgtaaatcc tagaccaaac
     5581 caatttttga tagattcaac agtgaaatga ggatctccca taatcccaat cacaaaacct
     5641 agagccattc cgataaagaa cttagagccc cagaagccaa tttttttgtt caatttagca
     5701 gcatcaaagt catatttatc tagccccgga aagaccttgt caaaaatctt atctaagacc
     5761 ataatgattg gattcatcat gtagttcatg tgagttgaag tcattggtga ttgagaccct
     5821 gtcccaagaa ggtcatcaaa ggtcggtttc atcaaatcag agttaatgat tttcaatacc
     5881 ccaacaagaa taactgccac agtagcaatc aaaagagaca caaggactgg caaatggttg
     5941 tgatcagcat accacataat caatagacct gtgatagaca aatgccaaat atcaaaaata
     6001 tcgacgtcaa gggtgtccgt ttttttcatg acaatcatga caatattaac caccagcatc
     6061 accagcaaga aataaagggt ataaggggaa ccccaggtaa ttgttgcaag cggtgcccaa
     6121 ccgacatcaa taatgtttaa actgatccct gtatttttaa caaaggcctg caaagactct
     6181 gaaaaagccc ccgtcaaaat cccaataatg gctccaatac ctgtaagagc gatagctaac
     6241 ttaatcccgc cttccagtgc ctttgtgaat ttaaccccga aaatcatggc taaacctgtc
     6301 atgacaatca gcatgaccac acctgatccc aactgaatag gaaatcccag aatcttgtta
     6361 ataatgtcta aaaaaggttg catcatcttg tctccttatg tttttattaa ctcactgcaa
     6421 atggttttct ttaatcacgc gctctaattc agcaaaaaca ggctcactca tgattgggat
     6481 acgatagaga atcggtcctg cttctactgt gggaattggc atctcaaatc ccaagtcagt
     6541 ttttgcaatc ggcgtaaaaa tatcataacg agacaccaat tcttctgtga tgtctttgac
     6601 catcaccgca tcacaatgca catcatagcc acgatcactc atctgagttt caatcgcatc
     6661 tttaatttga tggcttgagt taacccctgc accgcaggct gctagaattt taatcatgct
     6721 gaaacactcc tttttctatt aaatagtttg aaatagcttg tttatcatct tttagactgg
     6781 ctagatagct aagattgcct ttcgtaataa agaaggtaat gagattggat agaatcgtcg
     6841 tttgaccctc gttttgacta ttgataataa agaagaaatc cgttaccaaa cagtcttcct
     6901 ctggattaat catgtgctta aaggtaaggg cttgactatt tctaacatag accacttgat
     6961 caaccaagca atacttggtt tctgtatgcg gaatagccgc atacaaaata tctgacccat
     7021 ctttgaggtc cactttgagt cctgtcggaa ataccgcctc acgctccact aaagcctgat
     7081 gatactcttg cgtcacatac ccaacctttt ccaagtagct agcaacaaga tcaaaaagct
     7141 ctggctgcgt ccttgcttca gtaaatagaa tattaggaaa cactccctac cgcctttcta
     7201 taagtatcaa tttttaacaa ctgtcatctc atcatcaaca aattggtaac cgtttacaaa
     7261 atcattatag tataaccttc aacataattc aacattttta cgcaatattt tttgttctat
     7321 atgtttgttt ttgttttgtt tattttgttt tattgaaata tgaaggtcta tatgctatga
     7381 taaacataag taaataaact atagaaagga gccccttaga tgttaaaaag agaaagactt
     7441 ctaaaaatta ccgaaatagt caatgagcaa ggtattgtta ctgtgaatga tattattcag
     7501 actttaaacg tttctgatat gacagttcgt agagacttag atgaactgga aaaagccggc
     7561 aaattgatcc gcatccatgg aggtgcacaa agcattacca tgcctaataa aaaagagcgg
     7621 tcaaatattg aaaaacaaac cgttcaaaca aaggaaaaat gggaactagc ttcatatgcg
     7681 acacaactag tcaatgatgg tgaaacaatc tttattggtc caggaacaac tttagaatgt
     7741 ttcgctgagc aattaaaaaa tcgtcagatt cgcattgtga ccaatagcct ccctgtcttt
     7801 aacatccttc aggacagcga aaccattgac ctgattctta tcggcggtga ataccgatct
     7861 attacaggag cttttgtagg gtcccttgcc agtcaaaata tctcatcctt aaaatttgcc
     7921 aaggctttta ttagctgtaa tggtatttat aaaaacgata ttgcaactta tagcgaaact
     7981 gaaggagaga ttcaaaagct agcttttaat aattctattg aaaaatacct cttggtagat
     8041 aaccaaaaat tcaatgccta cgacttcttc attttttacc atttaaacaa cattgatgct
     8101 gttgttacag attcgcaaat aactgaggac gtcatcgaac gttatagtca gttcacacaa
     8161 ttattagtag cccacgaaaa caaggagtaa ccgtttcatc aagaatggac agcctgtcag
     8221 cgcatcgtcc ttaaacacct tctggcaaaa gtgatttgct tccagagagg tgtttttatt
     8281 ttgctcggaa cacacctgct aacgtcagag tctttatgag tgaccacaaa aaaagaagcc
     8341 cagattaaaa aactggaacc tcttttgaga tgaagtaagg ctaccaaaat aacctaagat
     8401 gattgatgta attcaaattt ggtatccttc aaagcacgct tgagtaggaa cgttaaagga
     8461 cgtcctgtca ccttagcctg tcctttttcc aaatttacct ggaccgattg cacaccgggg
     8521 acagctgata atttttcagt gaccgttctt gcgcaaccgt cacatgtcat tcctgtaacc
     8581 tgataatgtt tttccatttt gtattctcct ttaaatggtt tttcctttta atcgcagggc
     8641 attcaaaaca actgatactg aactaaaact catggcaaaa cctgctaaca tcgggttcaa
     8701 taatgggcct ccaaaaagat gaagtaagcc catggccact ggaatcatga ggacattgta
     8761 aatgaatgcc caaaataaat tttccttgac aatcctcata gtcgctcgac tcaatgacat
     8821 agctttcacc aaatccagca tatcaggttt catcagaatc acatccgccg attcaatggc
     8881 aatatctgtc cctgacccca tagctatccc aatgtctgct acagccaatg ccggagcatc
     8941 attaatacca tcacctacca ttgcaacttt tctcccttga gagcggaggt ctgctatcac
     9001 gcctgctttt tgatcaggta agacttgact gatcacatct gtaatcccca cttgagaggc
     9061 aatagcctta gcggtagcat cgtggtcacc tgttagcatg atggtgtgaa ttcccagttg
     9121 atgcaaagct tcgacggtag cttgactatc ttctttgaca gcgtcagcaa tactgaaaag
     9181 agctcgtagc tgcccctctt tagcataata tacaagggtt tggcctttct cagaagcctt
     9241 agcacaaact gactgaacac aagataaatc gataccaaga attcccatta aagcagcatt
     9301 tccaatcgcc aaacgacgcc cttcaagctg accagtaact cctaggccgg ttaaagactc
     9361 aaactcttcc actggtagac tatcgtaacc agcttcctga gcagcacgaa caatagcctc
     9421 ccctaagggg tgttgagaat aggcctccaa ggaagcagct tcttgcaaga taaggtgatc
     9481 atctcccgaa aaagttagga gcttttgaag acttggtttc ccctccgtaa tcgtccccgt
     9541 tttatcaaaa acaaccgtat ctacctgatg ggcttgttct aagatgtcac cacctttaaa
     9601 aagaatacca ttttcagctg cacgccctgt tccaaccata atcgctgtcg gcgttgctaa
     9661 accaagcgca caagggcaag caatgactag aacagcgatg gctgttgtca aactaaaacg
     9721 aagactttcc tgcatgaata ccaaccaaaa cacccctgtt aacagagcta ataccaaaac
     9781 caccggaaca aagacacctg agaccttatc agcaatagct gcaataggtg ctttattttc
     9841 ttgagcattc tcaactagtt ggataatttg tgctaacaag gtatcattcc caagcttatc
     9901 tggtttaatc gtgagggcgc ctggcccatt caagctgccc gcaaagacat tatctttttc
     9961 tcccttttca acaggcaaac tttccccagt taaaaaggat tcatccaaac tgctatgtcc
    10021 ttccacgata ataccgtcaa tagaaacttt ttcacctggt tgaaccagga caaggtcatc
    10081 atagctaacc tcttctaacg gcaacaattg ccattggcta tttctccata ccctcacaac
    10141 agtagccttc aaggataaga gcttttgaat agcttgggaa gtacggcctt tagaacgact
    10201 ttcaaaatac tttcctagac caatgagtgt caaaatcact accaccgatt caaaataaag
    10261 ggagtgcaca aagttggcct ggcccaaact gatttcaacc actccataaa gactataagc
    10321 aaaagcaaag ctggttgcta aagctaccaa ggagtccata ttaggatatc ccttaaacaa
    10381 agctttaaac ccattatggt aataatgacg atttaatact aagactggaa tcgtcaagag
    10441 tagctgacta aagctgtaaa cgaaaggata gcgatctggt tgcagaaatg gaggaagcca
    10501 taagcccacc atgtgaccca tagctaggta taatatagga atggtgaata gggcagacca
    10561 caagacctga gaccaaataa cctttaggtc ttcgtcacga cgacctgttt gcatgtgttt
    10621 tgcatgttca tcaaagactt gtgcaccata accagcagct tttaccgctt gaataacttt
    10681 ggccgtttct aaatcgtcat cttgataagt aaccgtgagt ttctcggtcg ctaaattcac
    10741 aacagcctgt tctacttcag gcaattgatt gaccattttt tcaattgtta gagcacaaga
    10801 ggcacaactc attccctcca ctaaaaatat ctctttagcc attaatacgt ctcctttttc
    10861 tgacagtggc actgcccagg caggcaatta cacgacacct caagaactgc attttctttt
    10921 ttgacttcta gtagctcttg gaatgcctca atttcctcta aggtcatcgg taagtcataa
    10981 agccgttcaa gaagtagatg cgtgtgttgc cgctgacaaa taccgtctaa aacagtcgct
    11041 agtgcttctt tttgtagcaa agtctcatcc aaaagagctt gataaatata agctcttcct
    11101 tgccggtaag ttcccaaagc ctttttttcc accaaacgcc caatcaaggt tttaatggta
    11161 gaatctgacc attgatattt tttccttaaa atagtaataa tatcactact tttaatatca
    11221 ccactggccc acaccacacg catgacttcc cattcggcag ctgaaatatt ttgattcatc
    11281 aactcacaac cttttaatct acatttgtag atatattata tcatatttat ctacatttgt
    11341 aaatatataa agtttctaat gattttattt cttaagttga caattttttc atttatgata
    11401 tgattatatg ataaaaaaat gataataata aggagaactc attgaaaaag acacttaaaa
    11461 gcactcttat tttactagga cttactctag caataggagc tagcactcaa gttgatgcca
    11521 gttctagatc ttggaaaagc tggtttatag agaaatactt ttggttaaaa ggagacaaag
    11581 gctattattc agagcaagac gaaactaagt ttgatcaata tgtcaactct gctggagaag
    11641 cttcaaataa aggctttgat cttaaagaag aacaggtaaa tggcacatta gtcactaaaa
    11701 ctgttgataa tatgcaagtt catacttgga atgagaaccc cagcaataaa gccaatcaaa
    11761 aggtcatttt ctatttacat gggggctctt acctaaacaa cccaactact tatcatatcg
    11821 acatgctaaa aaccttatca actagtcttg atgctaagat tatcttacca atctatccaa
    11881 aagcacctac ttactcttac agtgatgcta tgccaaaatt ggttgacctt tacagagata
    11941 ctcttaaaga aacaactgca caaaatatca ctattatggg tgattcagca ggtggtggac
    12001 tagcgcttgg tttagcatta gccctaaaaa atgaaagttt aaaacaacca aaagacattg
    12061 ttctcttatc accatggtta gatgtaacca tgtcaaatcc agatattgag agctatgaaa
    12121 gtaatgatcc cattctttca gcttggggcc tacgtaaagt tggggaaatt tgggcacgcc
    12181 gtgacccaaa caacaccaaa catccatatg ttagtccaat taatggagat gtatcagggc
    12241 tagcacctat tactatgttt gttggtacac atgaaatttt ctacccagat gttaagaaat
    12301 ttgatgatat attaactgcc aaaggtcatc aacatgaact tatcgtcgct gataaaatga
    12361 atcatgttta cccaatctat ccaattgaag aagccaagac tgcacaatat caaatcattg
    12421 atacgattca aaaagcttac taaaaacaaa aaacccttaa ggtgactcta ataagatacc
    12481 ttaaaggttt ttaatttaat cttttttatc aagctcacgt aacagttgat ctattttatt
    12541 accataggcg atggaattgt ctttttcaaa caccaaatct ggaattttgt acatggtcaa
    12601 ttgtttgcca agttcacgtt taatcgttcc agtcgctttt tcaagccctg tttgtgcttt
    12661 ttgattatca gatgccaagt cactcataat agtgtaataa actttagcaa gtgacaagtc
    12721 accctgcatt tgcacttctg taatcgttac tccctgaacg cgtggatcac gcactttttt
    12781 ctgaagaata tcatttactt cgcgcttgat ttccatccca acgcggtcaa tacgatgatt
    12841 agccatagcc atttcctttc cacattttta aaacaaaagc taggctgaaa gcccagctta
    12901 tgacattgct acctgttttg ataacgacaa ctaaagggaa tctgagaatt ccatgacgag
    12961 actggaagga atgccccaag tagctcatct ggtagaacaa gtcattattt ccgacagagg
    13021 aaaaacgtca catctcagac cttcttagcc cctattttct cacaatttct tccataatat
    13081 aggcttcaat agtatcgtca actttaagat cgttaaagtt ttcaatcatc aacccacctt
    13141 cttgagcatt accgacttct ttaacgtcat ctttgtaatg tttcaagctg gccagtttac
    13201 catcaaaaat aacgacacta tcacggataa cacgaacact cgaatcacgt gtcaccttac
    13261 cgttgattac cataaagcca ccaattgttc ccactttaga aactttgaag gtttcacgaa
    13321 taatggcttc accaagaatt ttttcttggt aaacagggtc aagtttccct ttcatggctt
    13381 cttctacttc ttcaatgact ttatagataa tgctatgaag acgaatttcc acatcatccg
    13441 tatctgcttg ttgacgagct tgtggggtag ggcgaacgtt gaaaccgata ataacagcat
    13501 ttgatgcttc agcaagggtc acatcagact cattgatagc acctacagca gagtgaacga
    13561 cattgacacg gacaccttcc acttcaatct ttacaagaga tgctgcaagg gcttcaacag
    13621 aaccttgaac gtctgctttg atgatgacat taacagtctt gatttctcct gctttaaggg
    13681 tatcaaagag attatcaagg ctaacacgct gtgtattttg acgttgtttg aggagggcac
    13741 gttttgatcg ctcctcacca gctgcacgcg cagctttttc atcagcgtaa acagcaaagt
    13801 gatcaccagc cattggtgtt tcgttcaaac cagtaatgga aactggtgtt gatggctccg
    13861 cagatttaac acgacgccca aggtcattta ccatggcacg gacacgacca aaggtattac
    13921 caacaacgat tgggtcttga acatgaagag ttccttgttg gacaagaagc gtagcgatag
    13981 cacctttacc tttatctaag cgtgcttcaa tcacagtacc aatagcacga accgtcggat
    14041 cagctttcaa ttcttctact tcagcaacca aaaggactgt ttctaatagt tcatcaatat
    14101 ttttattaaa tttagcagat atctctacaa attcgcactc tccgccccaa gcagttgaga
    14161 tgataccata ttctgccaat tctgcgataa cacgttctgg attagcacct ggtttatcaa
    14221 ttttgttgat agcaacaata attggaacac cagcagcttt tgagtgattg atcgcttcaa
    14281 tcgtttgtgg cataacacca tcatcagctg caacaatcaa aatggtgata tcggtaacgg
    14341 aagctccacg tgcgcgcatg cttgtaaagg ccgcgtgtcc gggagtatcc aagaaggtaa
    14401 ttttcttgcc agcttcttca atctggtaag caccaatatg ctgagtaatt cctccagctt
    14461 ctcctgttgc cacacgtgag ttacgcaaag tatcaagcaa agttgtctta ccgtggtcta
    14521 cgtgtcccat gatagttaca acaggtgcgc gttctacaat attttcagga ttgaggtaat
    14581 tttcatcctc aaagaagcgc tcgatatcag catcatctac ttctacctta gccttagcct
    14641 caataccata atctaccatc aataattcga tagtatcccc atctaaagac tggttttgag
    14701 ttgccataac acccatcata aacagctttt taacaatttc agcgggttcg cgtttgatac
    14761 gttttgcgat ttcggcaact gtcatgcctt cagtatattc aaattctttt ggcaattcat
    14821 ggaatttacg ctcggttact ggttttggag cggtgttagt attcttaaca tttttgcctt
    14881 ttttggtctt tttattttta ttccagttac tatttttttg atttctcact tggttttgac
    14941 tattccaact cttcttattt ttcttttgtc cttcttgtga atgatgagtt aactcacgat
    15001 tcttttcagg acgtgcttgt tttttacggc gattatcagc agtagattta gcactaatat
    15061 ttgatgactt taatttagtt tctgcttctt caatagattt cagtgctgct tcacgatggc
    15121 tcatctcctc agcttgtgct tttaaagccg cttctttagc ttcttgtctt gccaactcag
    15181 ctaaacgctt agcttcttgc tgtgcacgaa aacgtgtttc actttggcgt gagtattcag
    15241 cattttgttc agcttttaag gcagcagcac gagctttgaa atcaatttta ggacttttcg
    15301 attgaggctc ttgtcgtcct tgctttttct ggtttgtgaa tcgaccattc ttgtgattct
    15361 ccttagcaaa ggaacggtta cgatcaccaa ctagctgatg attatcatgt ctaggtgata
    15421 cattcgatgc ttttcgctca atacgcttat cgtttgtatc accttgagaa cgatgggtaa
    15481 tcctcttatc tccttgttcg ttagtttgat gattattggg ctggcgataa cctcctcgac
    15541 gttcttgaga acgatgagaa gagccatttg cttgttttcg agcagcctgt tcctttgcac
    15601 gagcctctct ttcagcttta aaattacgac tctttggctt agagacctgt gttttctcta
    15661 catgctcaga acctgtttct ttgaccactg ttacagaggg ttgagcaact tctttgggct
    15721 tgactgtttg agaagctgtc acatcaggtt tagaagcctt tgaaaaactt gaaataatct
    15781 ttttcgcatc cgcttcttca acacttgatg catggctttt cacatctaat cctaagtatt
    15841 tagcgtgttc aaccacctct ttactacttt taccaatttc cttggcaatt tcatgtaatc
    15901 ttttctttga caatttgtgt cctcctattc atctattcca taagagtcct cattttcttt
    15961 gaaaatccag catctgcaac ggcagcaacc ttccttggtt tacctagtgc agcacttaat
    16021 tccagtgcat tcaacactgt ggagacttct acattataat aattactttt atctgtcact
    16081 ttttttgtca cattaggacc agcatcattg gctaaaaata ctaatattac ctgctggtgt
    16141 tgaatagctt ttactactag ttcttcacct gaaatcactt ttccagcccg ttgagctaga
    16201 ccaataagac tagataatct ttctaaatta gtcaagacct aactctcttc ttttgatttt
    16261 gtgatcaaca taagcaatca agtcatcgta aaaactttct ggaatgtcca ttgagaaact
    16321 cctgttgaaa acttgttttt tcttggccat taaggcctct tgattgtcta atttgatata
    16381 agcaccacga ccattttgtt taccagttgg atcaatgaaa acttgaccat ccttagtttt
    16441 tacaatccta agaagatctc gtttagcaat aatttcacca gaaacaagtg attttcgtaa
    16501 aggtattttt ttgactttag acatcaaaca cctcttttct tacacttcct cggcagtcat
    16561 aacatcgtca acaactggct cttccacaac tgttgctgct tctttttccg cctcaagacg
    16621 atcatattcg ctagctgatt tgatatcaat acggtagcca gtcaaatgtg ccgctaaacg
    16681 aacgttttgg ccgcgacgcc cgatagctaa tgataatttg ctatcaggaa ctactaccgt
    16741 agcacgttta agatcttcat cgtcaaaaag taccatgtct acttcagccg gagcaatggc
    16801 attgtagatg aactcagctg gatcatctac ccattgaatc acgtcaatat tttcttcaac
    16861 tggaatttca agccccgtct tagcatctac acgttttgga tggaacttgc tgatgacctt
    16921 tttaatatta cttccaccac gtccaacaat tgttccgatg gcatcaacgt taggattatg
    16981 actgcgcaca gcaaccttgg tacggtcacc tgcttcacga gagacactca tgatttcaac
    17041 agtaccatca aagacttcag gaatttcttg ctccataata cgtttgatga attctggatg
    17101 gctacgacta acaaagacgt taactccttt aggattattt tcaactttgt agacataaac
    17161 gtcaatacgg tcatgtgatt tgaaagtttc acctggaatc tggtcttggt gagacaattg
    17221 tgcttctaag gaaccaagat tgacataaat aaaacgttga tcaaaccgtt caactgttcc
    17281 tgtcataatt tcaccttcat gctctttgta ttcattgaac atcacttcac gcatttggcg
    17341 gcgcatcttt tccataatag tctgcttagc tgattgagct gcgacacgtc caaattcatt
    17401 gacagattcc tcaaagcgaa tcttatcacc caattcatag gcagaactga tagcaagagc
    17461 atctttcaaa ctaatctcaa gccggctgtc aaaaacttct tcaacaacct cacgaaccgt
    17521 gaatacttga aaatcagctg ttttttcatt aaattcaata acacatgatt ctgattgacc
    17581 atagcgacgt ttataggcag actttaatga ttctgtcact gcgtcaataa tatctgcttt
    17641 atcaatgtgt ttttcttctt ccaaaatacg gaaggcttct agcatttctt tgctcataat
    17701 gttatctacc ttgcttgtaa agcttggcaa aaatcctttc ttaaattaat acgatcaata
    17761 gccccctaaa ggctccctta attccttttg tttagcttgt ataaactcta taggtgttgt
    17821 ttcaaatttg cttaagcgtt cctaaaaaag tttagtgctt tgaaataaaa gttataattt
    17881 gacagccatt ctcaccttag caactgcttg atatggtata ttcacgatct tatgacgtgt
    17941 tttatcaaga taatcaatgg tcaacgtttc accatcaaag gctagtaagt ctccttgaaa
    18001 aactttcacc ttatcaatag cttgataaag actcacattg atataagagc caactgctgc
    18061 ttttaaactg tcagctgtct taagcggacg ctccaaacct ggactagaga cttctagcat
    18121 atattggttt ggaaaaggat ctggatcaat cgtatctaac agcggactaa taatattcgt
    18181 caagtcagat gtatcttcca ctgtgattcc accttcctta tcgactaaaa tactaaggat
    18241 gtagtcacta cccattttgt catattcaac gtctactaat tcataaggtg ccttaatctc
    18301 aggtgttact gtcttggtta cgatatcgat aatagatgta tttgctattt tgattacctc
    18361 ctctattttt atgctcttct ctaagataat tggtccttgg gaatccactt aatacaaaag
    18421 cggcgagaca tatcccacca cctttctgta ttatttaagc ccattatacc ataatttaag
    18481 aggaatgcaa ggatatgcac tttgttttac tcacaaggag aggaggggat tcgaaccccc
    18541 gagccccgtt aaggactaca cgctttccaa gcgtgcgcac taagccacta tgcgacctct
    18601 ccattactaa agaagttaat actgtatcaa ctcctttatg ttatcatatt ttgaaacaaa
    18661 atactactag aaatttgcct caactcggta aatgacttga cctttattag agaacttctc
    18721 ttcatattct gtcatgacat ttccttcata gttactagcg tgtaagtcaa gccagatctg
    18781 tctcaaggtc atgccgtatt gagaaaaact agctaaactg tactcaaaca agccccgatt
    18841 atcggtttta aaatgaattt caccgtgttc tggcaagata cgcttgtaag tgtctaagaa
    18901 atccttgtag gtcaaacgac gtttttcatg cttggttttt ggccaaggat ctgaaaagtt
    18961 gagatacatc atatccacct caccatcttc aaaatagttg gttaaacttg atccatccac
    19021 tcgcagaagt ttgacattgg gtacttctga agctaatacc ttatccaagg cgtagcttag
    19081 gacagataat tgaatatcaa tgccaatgta gttaatatca ggatttttca aggccatccc
    19141 tgtgataaat ccgccctttc ctgaaccaac ttcgatatga ataggacggt catttccaaa
    19201 aacgtcatgc caacggccct tggcatcttc tggatttaat atcacatagt gtgggttatt
    19261 agctaagtgc tcctcagcac cttttcgttt tctaactcgc attatttctt tctaaatatt
    19321 tctctaaatt ttctgagggc ataaatctcc tgattcacat gctccatgtc acgcttgtca
    19381 aaacacttga gaatttgtgt caggtgagaa aattgaccat accaaataat tttttgcata
    19441 accttgtcat tatttttata gccataataa gacagccatt ctgaccaacg agaccgtgga
    19501 atatagtggc tcaacaggta agcaacatca tacatccgat cagtcagacg aacagaatcc
    19561 caatctacta aaaaaatcat accactagta gtaatcaccc aattgctatg tttaatatct
    19621 ccatgcacaa tcgttgctac ttctgatttg aactctggta agctccgttt taattcttta
    19681 acgatagctt gtaagtatga attttgctga atttgcaagg gtgcattttg ctcaaaatca
    19741 accaataaat cgtatgggtt ttcaatctta taattgagct gaagcagttg attgactaat
    19801 tttttagatt tgtgaaggcg caatagaata tgaatgattt gcttactgtt catatcttct
    19861 ttggtcaatg tacggccgtt aagccattct tgggcactca tcatatcacc attgcccatg
    19921 cgtttggccc aaagtaactg tggcgcaatc tgttcttttg ctaaggcagg tagaataggg
    19981 gtcgtattta attttataaa gacacattcc ccatttggat aagtgccttt ataagctttg
    20041 ccacttttcc cacgtaaggg agtcaaggta agttcttgtt ccgtcgttgt caccttatcg
    20101 ccctcctttt ctatctttta tagtcgttta cccctatttt acttgtttta ggctacttag
    20161 tcaatgatct tctttaactt ataaggtaga tatatacaag ctatgaaaac catacctaca
    20221 atcaaaatga gtgcttttgg cgctgatgag caacacagca tattgacgat taacattaaa
    20281 aagcttaaac ctcgtaaaaa acttaacaca tttttcttct tgctccgact attttcagga
    20341 taaagactag tcatataatg gtaatcgtag tgataataaa gtgaaagtaa ttggaataca
    20401 actaaatagt taaaaatgag tgctaaactt actgacaaat aaagattatg gataaaaaat
    20461 acagataatg agcttaatag cattagtcgt aaaaataagc ccaaataatc tgaactacgt
    20521 aaaaaggctc tagcataaag ggatagccaa aggtttgaag gagtttgctt aactaatttt
    20581 aaaagaggat ttagataagt tctttctttc acttttgtag aaattccttt gactgttgta
    20641 aaaagagaat aaaactttaa aatactttgc ttgcgattgc tttcaaatgc gacagctttt
    20701 gcccaatcta aacgatttcc tctcaaaaaa tatcttgtct ttcgactaag cactactctt
    20761 tttagtgcca ataatatcaa aactaagata ataaaaataa ataaagacag acctagacgt
    20821 ctaaaaatag gatataaaaa gagcaatata gcagtctgca aagttcccca aagcaaaaaa
    20881 gaaagtcgtt cagcttgttt gatataagaa atcacttctt cttcttttgg aagtaagaaa
    20941 tgctgatctg cctcttctag atacgttgct atagatccca tactgagcaa taatgggatc
    21001 aatatcccaa gacaaacctg aataggtaga tgattcgttg gaaaatggtt tagtagctga
    21061 ccatactgca ccatagcata gcctaataaa aacatcaaca ccaaaacaaa gtgatcattt
    21121 aagacgtaac gaaggtattt gttttgttgt ttttgaaagt cttgacggcg tttcagaaat
    21181 aatgctttca tacttaatcc tcttttgtta atgcaagata gatatcattt aagctcgctg
    21241 aacggtcacc aaaagcttct tgcaagtcag caagagttcc ttgggcacga acttgtccat
    21301 gatgcaaaat cacaaagcga tcacacattc tttcagcaga gtccaaaaca tgtgtactca
    21361 tcaaaataga ttttcctttt gctttttcga cttctagcgt ttggattaag tctgaaatag
    21421 ctaaagggtc taaccctaag aatggttcat caagaataaa tagacttgga tcaatgacaa
    21481 aggcacaaat aatcatgact ttttgtttca tccctttaga aaaattgaca ggaaaccatt
    21541 caagtttgtc agttaaacga aacatttcca agaaaggttg tgctcgtttt tgtgccactt
    21601 ctaagtcgat atcataagcc atagctacgg tgttgatatg ctctgataag gtcaattctt
    21661 catagagact aggagtttct gggataaaac ctattttttg acggtaggca actgcatttt
    21721 ctgctagagt taaaccatca atagaaatac ttccttgata aggcttcaaa aagccaataa
    21781 tttcgttgat agttgttgac tttccagcac catttagccc gatcaaaccg actaactctc
    21841 cattatctac agaaaatgag acatcgttta aaacagggat attgtgatat cctcctgtta
    21901 gatttttaat gtttaacata taatttcttt ctagtttttg atatacttat aatatattat
    21961 aacaaattca agtagagaag gtgatataaa atggaaaatt gtattttttg cagcatcatt
    22021 caaggggata tcccctcttc gaaagtctac gaagatgagc aggtcctcgc ctttttagat
    22081 atctcacaaa ctacaaaagg gcatactcta gtcattccta agcaacatgt tcgtaactta
    22141 ctagagatga ctgctgagac agctagtcat ctctttgctc gtattcctaa aattgctcgt
    22201 gctatccagt tagctactgg cgctactgcc atgaatatca ttaacaataa cgaagcctta
    22261 gcgggacaaa ctgttttcca tgctcatgtt catctcgttc ctcgttataa tgaaaaagac
    22321 ggtatctcaa ttcaatacac cactcatgag cctaactttc ctgtcttgga aaagctcgcc
    22381 cgacaaatca atcaggaggt tagttcatga aagttaaaac cgtgatagcc gtcggattat
    22441 tatcttttac cgcttacaaa gcatatcaaa aacgttgtac cataaaagag ctgttatcca
    22501 tcagtcgaca agcaaaagat gctgcccaac ttgatttaga taacattaaa gcaaaccttg
    22561 acctcattca cagtcaagga aaagtaattc aaaatattag tcaggacttg gcccataaat
    22621 ggcgctactt taaccaagag acacaagcac accttactga aatccaaaat cgtatggcca
    22681 aatatcaaga agatagtgag ctaatttcta gtgaatcacc tacttgccaa accgtcattt
    22741 taactaacaa ccattccaga tagtattatc tccctcatat actttatttg aaatcacaga
    22801 cagctatcat ctttgatacc tgtctttttt atattcaaaa tcggatattt taaacataaa
    22861 aaccctcaga acgttaggtc tgagggttga tattagcagg aggaactaat gatccagacc
    22921 ctgatgagct agactcatca gctgctaaag catctgatga agaagacggc gtagttggcg
    22981 tatggcttga agcagtagag tcctggtctg tactagcact ctgataagat gagccagtat
    23041 ctactccact tgaataatta gcgtaagagc tatgactatc agaataagat ggagcctggc
    23101 ctgaagagtc atagttgttg tttactgtct gagacttagt tgacccatac aaattttcat
    23161 aaacagtagc atttgttttt aattgactaa ccttatgaag tcctaattct gttcggatac
    23221 gattttggat ttctaacaaa tgattagagg taacaatttg gtatgatcca ccatctgata
    23281 aagtggcatc ttctcctttt agttgataag ttttaatagt tctaagtgcg tcacgataac
    23341 ctaatagact agggatagtg cgagaagaga tttcgatatt cgtttgcata ttactactta
    23401 cagcagataa aatcttccga taagagctaa tgctatcaag agcaaggatt tttttcaata
    23461 ccttttgaat gacttcacgt tgacgctttt gtcgaccata atctccctca ggatcatcat
    23521 aacgcatacg agcataaacc aaagcttgtt caccgttaat tttgtgtgtt ccaggcgcaa
    23581 cagtagcttg atattcaggt tcgttttcag caatcgagat aggaaaatca aactcatttg
    23641 taactgtaat ccctccaact gcattcacaa gatcaataag gccttgcata ttaatttgaa
    23701 catagttatc aatggtgata ttcaaaagat cttgcacggt cataatagcc atctgagcgc
    23761 cacctgctgc ataagcagcg ttaagcttag cttcaacacc attcatttca ttatttttgg
    23821 gtccagataa cgtggttaag gtatctcgtt ctaaactagt catagttgtt ttcttggtct
    23881 ttggattaac cgtaaccaaa atcatcgaat cactgtttcc ttcccacttg gaggcacgct
    23941 ctgaagagcc tgtatcaaca cccatcaaca agatagaaaa agctcttgtt tgtttaatgg
    24001 catcactttt ctttgaagat gtcgaaaaat ctttaaaggt ctttgataat tctcctgttg
    24061 agaaggtata agcactagtt agatagacac ctaatgccaa gacagttgtt aacacaatag
    24121 ctgtgaacat taaaactatt ttttttccaa ttttcatcga ttcacttact gacttacaga
    24181 tttacgcata tgataaacat ctaagtcatc tccatttttt gatttgatgt cgttttttcg
    24241 catactttca atacgaaaac catacttttt atacaggtaa atagccttag tatttcttac
    24301 ttgaacgtcc aacttaaggc tttcgatata agggttttct tcagcccaat caagagcaat
    24361 ctctaacaat aattgaccga taccataacc acggtaagtc ttagcaacta acataaatac
    24421 atctgcttca acttggcctt gggataatac ttccccagaa agatttaaaa gccctactac
    24481 tttttcatct aatctagcta gcaggcaaaa atcaataaaa gaggtttgac ttcttgataa
    24541 aaagtctgat aattcatgag gagtagcctc taatatagtc tctacgcctg ttataaaatc
    24601 tgtttctttg gcagctgccg ccactaagga aagtacagct tttgcatctt gaggtacgcc
    24661 ttcttcaata acgagacttt gctgaatcat gtgacattat ctccagaagc tgacgagagc
    24721 gctcaccatg cgctaacaag tctagttgac gacctttatc tcgtttagta atggttattt
    24781 gcaaatagtc ttgtaataat ccctctccta ataattctcc ccactcaata acggtaacac
    24841 cattaccaaa aagaaaatca tctaaatcaa tagagtctgg atcatcacca acacggtaaa
    24901 tatctaagtg ataaagaggt agacgtcctt catattctct aacaatagta taagttgggc
    24961 ttttgatcat ttgactaatc cccatccctt tagcaatacc ttttgccaat gttgttttac
    25021 cggcacctaa gtcgccagac aatacaatca catctccaat agacaaataa gtacctaggg
    25081 tttctccgta agctttaaga gtatattcat tttcactata aaacatagca acattatatc
    25141 agaaaaaaaa catacttcca agcactacct agaagatgac tccaataagg tattcgtaaa
    25201 gtaaaaacgc ttcgttatat taaaggttaa aatcaattaa agaagcggca gttagtagca
    25261 aaataagtaa attatttggt tataagatag ttaatatgat gaaatttaca atgaacaaga
    25321 aggttgctcc ccaaataata gggtgaatat cttttgtttt tccctcaaca acttttacta
    25381 gacaatagaa aataaaggca gcggcaatac cataggaaat agagtaacac agcgccataa
    25441 agaaagctgc aaaaaaagct ggaagagcat ctgcaaattt actccaatta acatcaagaa
    25501 aagaagacac catcatgaca cccacaacaa ttaaagctgg agccgtagca gcagctggga
    25561 caatacctac aagcggtaat agcaatattg ataataagaa gcatactgcg gtggagactg
    25621 ctgttagtcc agtacgtcca ccttcagcaa ttcctgctgc tgattcaaca taggtagtcg
    25681 tatttgaagt tccaaccaaa gccccaatag aagtaccgat agcatctgca aaaagcgcac
    25741 gatccatttt tgaactaaag cctatactat tttccaaagc attctcatcg tcttgagaga
    25801 aaatacctgt tcgacgacca gttccgataa aggtaccaat tgtgtcaaaa gtatctgata
    25861 gactaaaagc aaaaatagtc atgaaaacta gcggtaaacg acttgaatcg ctaaaaagag
    25921 aactcaaacc atcgaaagct gcaaggaaag ttgtacctaa ttcagtccaa gcttcaccaa
    25981 tatggctgat cccaaaattg aggttggaca catccactat tcctaaagga atacctacaa
    26041 gagttgttgc gacaatacca attaaaatag cacctctaaa atttcgaatc acaagagctg
    26101 tcgttaaaag taaaccaaaa atagcaagta atacactaga atccgtaaaa ctagaaatcg
    26161 taggtacaac tccaccattg gcgtctacaa ataatagacc atctgcaaat gttttagccg
    26221 atgctttagc cggttcaaca ccatttacca ttactatatt ttcagcagag atagaaaaag
    26281 taatgatatt tgcgttttta aatcctaaat aagctacaaa gacaccaatt cccccaccaa
    26341 tagcatgctg taaactaact ggaatcgcct tgatgatact tttacgaaac ttggttacgg
    26401 taataaaaat attgaataat ccacaaatga aaaccattgc caaggcttct tgccatgaaa
    26461 accctaaagc aaaaacaact gtataagtga aaaaagcgtt aagtcccatt cctggtgcca
    26521 acgcataagg cacattggca aatagtccca taattaaggt tgatatggct gctgcgataa
    26581 tcgtagccaa aaagacagca ttagagggca tccccgctgc acctaaaata cttgggttaa
    26641 caaacaaaat ataggacatg gcaaaaaagg tcgttaaacc agccataatc tcagttgaga
    26701 cagttgtccc attttcgctt aacttaaaaa acttttccat tgaaatggag tctccttaat
    26761 taaatagtag gtaaaatcaa aaagcgctta tcactagata agtctttttg acacctcatt
    26821 attattgtta ttgttcgtat taaaatgaac aataacacta tgttaccatt tttttgttca
    26881 cttttcaacg tctgtaactt catttattca ttaaaaaaac agttatccta tcttttttac
    26941 taatgaatac gcttaactta tcttttctct ttatcttgga gcaaaatttg ctataattta
    27001 attatgacta aaaaattgat tgccattgat ttggatggaa ccttattaca tcatgacaac
    27061 accatctcta cctatactca aaaaatcatc aaagctgtcc aagacaaggg ccaccacgtc
    27121 attatttcaa caggccgtcc ttaccgcatg gcactggact actatctgca gctcaattta
    27181 aagaccccaa tcattacttt taatggggcc ttgacccaca tgcctgaaca aaaatgggcc
    27241 tatgaacata atgtcacttt ggacaaaggc tacctactaa ggttactaaa atatcaggat
    27301 gatttccaga tggattttat tgccagtgag tatcgaaaaa atgtctacat caccatgacc
    27361 aatccagaaa gtatcgaccc tcaactcttt ggtgttgatg aaattactca ggacatggca
    27421 ttagagatta ccaaaatcac tcgaaatcca aacgccttat tgatgcaaac gcatcacgaa
    27481 gacaagtatg ccttagctaa aaacatgcgt gctttcttca aagatgaaat tgaaattgat
    27541 tcttggggag gccctctcaa cattcttgaa atttcgtcta agaatgtcaa taaagcttac
    27601 gctctgaact atctccttgg aatttacaat atggataaaa aggatttaat cgcctttggt
    27661 gatgaacaca atgacactga gatgctagct tttgctggaa ctggttatgc tatgaaaaat
    27721 gctagccctg tgctactgcc ttatgctgat cagcagctta atttttccaa cgaagaagac
    27781 ggtgttgcca aaaaattgga agaacttttc ttgtagtagc aaacgaaacc actatttttc
    27841 agaaaatggt ggtttttctt ctgtcaacag ctatcaaaaa atgaaaatca ttttaataat
    27901 ttcaacattt ttcagcatat ttttgtttta aacagcttga aaacaccttt aatatcaata
    27961 atctgcctat ttaatcctaa attctgaaaa ttttttcatg ttttgtacta tttttccgaa
    28021 agggcttgat tttttagtga aaacgtttta aaatagaaac gttcttgaat ttcgtttaag
    28081 tgaaaaagaa ttgccaaaat ttagttttgg aaggagatta ttcttcttca tttcaacgaa
    28141 aaatatttta aaggaggaag aacaagaatg ggtatcggta ttattattgc cagccatggt
    28201 aaatttgctg aaggtattca tcaatctggt tctatgattt ttggtgaaca agagaaagtt
    28261 caagttgtta ctttcatgcc aaacgaagga ccagatgatt tgtatggaca cttcaacaat
    28321 gctatccaac aatttgatgc tgatgatgaa atccttgtgc tagctgacct ttggagtggt
    28381 tctccattta accaagctag tcgtgtggca ggtgaaaatc ctgaccgcaa aatggcgatc
    28441 atcactggtc tcaacttgcc tatgcttatt caagcttaca ctgaacgtct tatggatgct
    28501 ggtgcaggtg ttgaacaagt tgctgcaaac atcattaagg aatctaagga cggtatcaaa
    28561 gcccttcctg aagaccttaa cccagttgaa gaaactgctg caactgaaaa agttgttaac
    28621 gcacttcaag gagctattcc tgctggaact gtcatcggag atggtaaatt aaagatcaat
    28681 cttgctcgtg ttgacactcg tcttttacac ggtcaagttg caacagcatg gactccagct
    28741 tctaaggctg accgtatcat tgttgcttct gatgaggttg ctcaagatga ccttcgcaaa
    28801 caattgatca aacaggctgc acctggtggt gtcaaagcta acgttgttcc aattagcaaa
    28861 ttgattgaag cttctaaaga tcctcgtttt ggtaacacac acgcacttat tcttttccaa
    28921 acccctcaag atgcccttcg cgcggttgaa ggtggtgttg aaatcaacga attgaacgtt
    28981 ggttcaatgg ctcactcaac tggtaaaaca atggttaaca acgttttatc aatggacaaa
    29041 gaagacgttg ctacttttga aaaattacgc gacctaggtg ttacattcga cgtccgtaaa
    29101 gttcctaacg attctaagaa aaacttattt gagcttatcc aaaaagctaa cattaaataa
    29161 gtttcaaacg ctataaggtt ttaaaacgat gactagctaa cttattagca aaaagtcctc
    29221 gtttactata ctttgaaagg attacaacat gtcagatatt tcgattattt ctgcaatttt
    29281 agtcgttatc attgccttct tcgctggtct tgaaggtatc ctcgaccaat ttcaaatgca
    29341 ccaaccactt gttgcctgta ctttgattgg acttgtaact ggacaccttg aagctggtgt
    29401 catccttggt ggtacacttc aaatgcttgc tcttggttgg gcaaacatcg gtgctgccgt
    29461 tgcccctgat gctgcccttg cctctgttgc agctgctatc atcatggtca aaagtggcga
    29521 ctttactcaa aaaggaatta cctttgctta ctcaacagct atcccacttg ctgttgctgg
    29581 tcttttcctt acaatgatcg ttcgtacttt gtcaactgcc cttgtacacg ctggtgacaa
    29641 agctgccact gaaggtaatt tcgcaggtat tgaacgcttc cactttattg cccttcttct
    29701 tcaaggattg cgtatcgctg tcccagcagc tcttcttgtt gctgttccta cttcagccgt
    29761 acaatctgtt ttgaatgcta tgccaaactg gttgaacgaa ggtatgcaaa tcggtggtgc
    29821 gatggttgtt gctgtaggtt atgctatggt tatcaacatg atggctactc gtgaagtatg
    29881 gccattcttc gccctaggtt ttgctcttgc agctatcagc caattaaccc ttatcgctat
    29941 gggtgttatc ggtgtagcaa ttgccttcat ctaccttaat ctttctaaaa aaggtggtaa
    30001 cggtggtaat gcagcaggtt cagctgatcc aatcggcgac atcttagaag actactagaa
    30061 aggggtaaga acaaatgact gaacaaatta aattaactaa atcagaccgt caacgtgttt
    30121 ggtggcgttc acaattctta caaggttctt ggaactacga acgtatgcaa aacatgggct
    30181 gggcttatgc acttatccct gctcttaaaa aattgtatac gtctcctgaa gaccgtgcag
    30241 ctgctcttga gcgccacatg gaattcttta acactcaccc atacgttgca gctccaatca
    30301 ttggtgtaac acttgccctt gaagaagaac gcgcaaatgg tacgccaatt gatgacaagg
    30361 ctatccaagg ggttaaaatc ggtatgatgg gacctcttgc tggtatcggt gacccagtat
    30421 tctggtttac ggttcgtcct atccttgggg cacttggtgc ttctttggct tcaacaggta
    30481 acatcgttgg tccacttctg ttcttctttg gatggaacct tatccgtatg gccttcttat
    30541 ggtatactca agaatttggt tacaaagctg gttctgaaat tactaaagac atgtcaggcg
    30601 gtatcctcca agacattact aaaggtgctt caatccttgg aatgtttatc cttgcagtgc
    30661 ttgttcaacg ttgggtatct atcaacttta ctatcgacct tcctggaaaa caattgtcag
    30721 atggtgccta tgtggtcttc ccagatggcg ctgttaaagg tgctgagttg aaaactatcc
    30781 ttgccaacgc tattggcggt atgagccttg ataaagtcca agctcaaacc cttcaaggac
    30841 aattggactc tcttatccca ggtttagctg gattactcct tacattcctt tgcatgtggt
    30901 tattgaagaa aaaagtttct ccaatcgcaa tcatcatcgg cttgtttgca tttggtatcc
    30961 ttgctcacct tgcaggtatt atgtaatcaa ccaatggaag tctgagatga actcaggctt
    31021 cttttttatg ctttatgtca ggaaaatccc acttaaaata tggtataata actacaacta
    31081 cgcaatgaaa ggaacttaaa tggctcaatc tttaaacact agtgtcgaat acaagacaaa
    31141 agctgtctcc taccttggaa taggaggcaa agttggtcat attttactcg gtgacaaggc
    31201 ccttgaattt tacaacgata aaaatgtgaa tgattatatt caaattcctt ggacagctat
    31261 caatcacatc ggggctaatg tttcaagaaa aaaagttagc cgccactttg aaatttttac
    31321 ggaccaaggc aagtttttat ttgcttctgg agactctgga aaaatcttaa aaatcactcg
    31381 tcaacatatt ggtaatgaga aagtgattac attaccaacc ttgatgcaaa cctttattaa
    31441 caaatttttt cgacattaaa aaaataatga gcagtgctga tttatcattt caagaaagca
    31501 aagaatagta gctctaaatt gctatttagt ggaaaagatt ccctcatttg agtttacttt
    31561 ttcttgtttt ttgttataat agtcctaacg gataagtaat accttttttt cattcagaga
    31621 gtctgtggat gctgtgaaca gatagggaaa atgatatgaa attctaccgt gtcatcaata
    31681 aaatagagat taagtgcaca gattgtgaag ctggatggaa ccgcgccaaa gcgctccagc
    31741 atcttcataa gctgtgcttt ttctatcgtc agagaatcac aagatccaac tgtcttactt
    31801 agccttgttt cactgacagc tactaataac tatcttaaag gagcttctgt atgctagatc
    31861 ttaaacgtat ccgtaccgat tttgacactg tcgctgccaa attaaaaaac cgcggtgttt
    31921 ctgaagacac cctgacccat cttaaagaac ttgatgaaaa acgtcgtgct ctattagtgc
    31981 aatcagaaga attaaaagca gagcgtaata ttgcttctgc agccatcgcc caagcaaaac
    32041 gtcaaaaaga agatgccact caacaaattg ctgatatgca aaaagtctct gcagatatca
    32101 aaactattga caatcaacta gtagctatcg accagcaagt tactgacatc atcactgttt
    32161 tacctaatac tcctcacgat tctgtccctg ttggagctga cgaagaagac aatgtcgaaa
    32221 ttcgccgttg gggaacgcct cgtgactttg acttcgaagt taaggctcat tgggatctag
    32281 gcgaagatct tgatatttta gactgggaac gtggggcaaa ggtaactggt gctcgtttct
    32341 tattctataa aaatcttggt gctcgtttgg agcgtgctct ttataacttc atgctggatg
    32401 aacatatcaa agaaggttat caagaaatca tcacacctta catggttaac cacgattcca
    32461 tgtttggtac aggacaatat ccaaagttca aagaggacac ctttgaatta gcagatacca
    32521 actttgtcct gattccaact gctgaagtgc ctttgaccaa ttattaccgt ggggaaatcc
    32581 tagacggcaa agaattgcca atttacttta ctgcaatgag cccatctttt cgttctgaag
    32641 cgggttctgc gggtcgtgac acacgtggcc ttatccgcct acaccaattc cacaaagttg
    32701 aaatggttaa atttgctaaa ccagaagaat cttaccaaga actagaaaaa atgactgcta
    32761 atgctgaaaa tattcttcaa aaacttggtt tgccatatcg cgtcatttcc ctttgtactg
    32821 gagacatggg cttctcagct gctaaaacct atgaccttga agtctggatt cctgcacaaa
    32881 acacctaccg tgaaatttca tcatgttcaa atactgaaga tttccaagca cgacgtgctc
    32941 aaatccgtta ccgtgatgaa gctgacggta aggtcaaact tcttcacacc cttaatgggt
    33001 caggacttgc tgtcggtcgt accgtagcag ccattttaga aaactaccaa aatgaagatg
    33061 gttctgttac catccctgaa gtactccgct cttacatggg tggcgaaacc gtcatttcac
    33121 ctaaataata aactcacccc agagatcatt aaagtgattt ctggggttta ctattttaaa
    33181 gagccaccac atcacttgaa actcactaca ttgccccttt aacaacactt tttttcacac
    33241 tagtttatca aaagtcttat tagtataccc atttccttat ttacaagact agttaaaaac
    33301 ccttttggaa aggcctccct tagcttgata aacacgtagg gagactccaa aaggatgttg
    33361 ttaatattta cgaaagcgtt ggtaacgctc atctaataat tggtctaatg gcttagcttg
    33421 caaactggtt atttgttcga tgaggttagc tttgatgatg tcaacgattt cacttgaaaa
    33481 ataaccatgt tctggaataa tacggtctac tatgcccatt tgatacaatt cgcctgcggt
    33541 aattttcatc aattcagcag cctctgtcgc tcttgaacca tctttccata aaatagaagc
    33601 aaagccttct ggactaagga ctgcatacat ggtgttttct agcatccaga cctgatcggc
    33661 aaccgctaag gctaatgcac caccagagcc tccttcacca ataatgatgg cgataatggg
    33721 aaccttgaga tcactcattt ccatcaaatt tttagcaatg gcctcaccct gtcctcgttc
    33781 ttccgcaccg acacctggat aggctcctgc agtattgata aacgtaacaa ctggtcgtcc
    33841 aaatttttct gcctgtttca taaggcgcaa agctttacga taaccttctg gattgggctg
    33901 gccaaaattc cttgccaaat tatcctgtaa attcttacct ttttgaatac caatgaccgt
    33961 aacaggttgt cccgccaaat aagctaagcc acctacaata gcaccatcat ctgaaaaatg
    34021 gcgatcgcca tgcagttcca taaagtcatc gaaaataagg ttggcgtaat ccaaagttgt
    34081 taaacgccct tgatcacgcg cttcttttaa aattcttgat acatctgtca ttggccacct
    34141 ccatgaaatg ctactaagtg ggcaatcttg tctcgcaact ctgttcgttt cacaatagca
    34201 tccacaaaac catggtcttg taaaaattct gctttttgga aatcatctgg caagttttca
    34261 cgaactgtgg tctcaatgac acgacgacct gcaaagccaa ccaaggattg aggttctgcc
    34321 aaaataatat ccccttccat ggcaaaacta gctgttaccc caccagtagt tggatctgtc
    34381 aaaatagtga ggtaaaagag gcctgcatta gaatgtcttt tcacagctgc agagaccttg
    34441 gccatttgca taaggctcat gatcccctct tgcatacggg cgcctcctga agccgtaaaa
    34501 atcaccaccg gcaggttttc ttcgatagcc aattcaaaca atcgggtaat tttttcccca
    34561 acaacagttc ccatactagc cataataaag tgagaatcca taatagcaag agcaatttgt
    34621 tgtcctttaa ccatagcttt ccctgtcaaa acagcttcat gcagaccagt tgtttccttg
    34681 gctttttgta atttttcctg ataacctgga aagcgtaacg gatccttggt ttcaatactt
    34741 gtaaataatt cctggaaaga gccttcatca actgttaagg tcagccgctc ttgggcagaa
    34801 atcctaaaat tgtaagaaca ggttgggcaa atctttgcca atcccaaatc tttcttataa
    34861 atcatgtgct tacaagctgg gcatttcgca aagagttcat ctggaacttc tgggacatta
    34921 tggctgacag atcctttgag ggaattattg ggagtaatac ggatgtattt atctttttta
    34981 cgaaataaag ccatattttc tcctaatctt tattgtaatg tggcaggaac gtttccatca
    35041 agaaagaggt atcataatct cctgcaatca ctcgcttatc tgaaatcaaa tcgagctgga
    35101 agtctgtatt ggtgataatg ccttcaattt ccaattcaac taaggcacgt tgcattttca
    35161 tcaaagcgtc aaatcgattg cttccatgaa cgatgatttt agcaatcatg ctgtcataat
    35221 aaggaggaat ggcatagcca ttgtaaactg ctgaatcaac tcgtagtcct acaccaccac
    35281 ttggcatata caagtctgta atctttcctg gactcggtgc aaagttaaaa gcagtatttt
    35341 cagcattgat acgacattca atagcatgtc ccgtgatagt gatgtcttct tgactaatcg
    35401 ccaaaggctg acctgctgca attttgattt gctctttgac aatatccact cctgtcacaa
    35461 attcggtgac aggatgctca acttggatac gggtattcat ttccatgaaa tagaatttct
    35521 cgctgtcttc gtccaacaaa aactcaatgg ttcccg
//
LOCUS       DAUVUY010000007        34896 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.007, whole genome shotgun sequence.
ACCESSION   DAUVUY010000007 DAUVUY010000000
VERSION     DAUVUY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34896
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.007"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            57..926
                     /locus_tag="VOX58_000350"
     CDS             57..926
                     /locus_tag="VOX58_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HES5877617.1"
                     /translation="MYHTTALSFLKKGYQVIPLRKDTGTPMIKFKDIQITEEVIKNTN
                     WFNCDYALLMRGIWCIDIDTHDMDEKLAKELYIMIKKMGIDLLSVLSTDKYDNGLDGY
                     SSIIRHEYKNELISNFKNTFAELTASGGMHILFKKRDGINYTQKIGVMPGVDIKANDN
                     NFVKIFPSDGREVLQAVKTLPYYDGKFEEEAFKPKQEGITTYFGGSVTYTSNGSHEGR
                     EAYERVATGTSYNRNNDLFKGACWAFENGIDIDDLTSIIGTVKGRDVFTREEFELTIE
                     SAKRKVSYVTIRT"
     gene            880..1230
                     /locus_tag="VOX58_000351"
     CDS             880..1230
                     /locus_tag="VOX58_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HES5877618.1"
                     /translation="MNQRNEKSATSLSEHDIQNLIRMELSQAGHMVFRANVGKVKTAD
                     GRFFDTGLPKGFCDLFGFKPNGQIFFIEVKNETGRIRPEQKKFMEVMASRGALVGVAR
                     SVEDALNIVNDTSR"
     gene            1214..1570
                     /locus_tag="VOX58_000352"
     CDS             1214..1570
                     /locus_tag="VOX58_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877619.1"
                     /translation="MTLVDDFYKQMEPSIKAFLDDNITIADKEEADRVYRSVKYYKKL
                     NRLPPPDVLEWFQRIYTTKEMIMLIKQSYRLKQKKTDEDDKIYEKWMFKNYGDVKLVK
                     KIKRTDALERARRMGL"
     gene            1812..2048
                     /locus_tag="VOX58_000353"
     CDS             1812..2048
                     /locus_tag="VOX58_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HES5877620.1"
                     /translation="MIEELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLIEYWLIESVEE"
     gene            2045..2215
                     /locus_tag="VOX58_000354"
     CDS             2045..2215
                     /locus_tag="VOX58_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877621.1"
                     /translation="MKRYIEVKDEWKSATDHLNDFIDKNKYAKVTVVGYQVVQLSPYG
                     RDLTYILAEVEE"
     gene            2212..2496
                     /locus_tag="VOX58_000355"
     CDS             2212..2496
                     /locus_tag="VOX58_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877622.1"
                     /translation="MTIDEALQNLRDNFNKIMNVLKNDWKALLFLAIAIFGMMVTVSY
                     FSYRDARQYYESQITGLRTQLSRTQKQLKRASEDRARQTKRIAEFTHNGG"
     gene            2498..3130
                     /locus_tag="VOX58_000356"
     CDS             2498..3130
                     /locus_tag="VOX58_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HES5877623.1"
                     /translation="MIKIDEIHRILGIDEVYKAPKRLTDILFDKDSREDIFRQFLKYE
                     TDVSYDWFMQYFEEEQADRKNKKQDFTPKSVSTLLSKIISGNQYYEVAVGTGGILIQA
                     WQEQRLNDSPFTYRPSKYWYHVEELSDKAVPFLLFNMSIRGINGVVVHGDSLTRQVKN
                     IYFLQNTKDDMLSFSDINVMPRTQDIEQEFNVKEWIGDAIVHVESKLNAR"
     gene            3135..3614
                     /locus_tag="VOX58_000357"
     CDS             3135..3614
                     /locus_tag="VOX58_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HES5877624.1"
                     /translation="MNIEEAIKKIRELEAFNYFDFKGNLIRRIDVLGIIDQLKIIVQQ
                     CVADWYEKHKDNLEYDLYLYQMSIYDEKVEEDDFYYWMQKSKNPVRTLINMHQFGYEI
                     EQEQLYTVDLPNGQPLVRGKNTLYFSQNLTAENAKLTESEIRKDFEWAWQFAKEVTE"
     gene            4063..4497
                     /locus_tag="VOX58_000358"
     CDS             4063..4497
                     /locus_tag="VOX58_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HES5877625.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPHGVPSKPVERLALNRVSAEQELDAIEQAVSMILEPERRRILYDKYLAPYKKAD
                     KVIYTELCMSESFYYDTLDIALLAFAELYREGVLLVEQGVFS"
     gene            5075..5989
                     /locus_tag="VOX58_000359"
     CDS             5075..5989
                     /locus_tag="VOX58_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877626.1"
                     /translation="MENKPAKASFFKLSSSKESNLKLFIEKMEEMFDNFRKKQYSNIP
                     TLEINDLMYYINAMQKVTSEEELNGTNLFYWLVTISRVDTESPIILANLEKNIDVRKR
                     EIEHGDNEGLVVDTRLLFDPFRQILVVYNQRGTINNYDLRRFFCQIIGVRGLKFDIIL
                     NSDAFKRVGKLDVVNSISYTVASPTNFKEFRDDTQSENADLKFANSMLGESMQVVIKS
                     NHLSKKNIFDKFSDMLVNDSVNVKNAKVEGLTDGHPELIDLIKNKLEYKGTIFYENTL
                     DDEAVYAFLNTAYSFHFSHLKRMFTITL"
     gene            complement(6079..6291)
                     /locus_tag="VOX58_000360"
     CDS             complement(6079..6291)
                     /locus_tag="VOX58_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877627.1"
                     /translation="MKDKKVATNRRSIIFISVPKSSIITTNLLEPEVGINEKLAKNIA
                     VTIAENDRDADIISVKFFKLFGEILK"
     gene            complement(6607..6984)
                     /locus_tag="VOX58_000361"
     CDS             complement(6607..6984)
                     /locus_tag="VOX58_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HES5877628.1"
                     /translation="MLIYPATFTQDTDYIMVTFPDVPEAITQGEDFQEAYEMAVEVLG
                     FALEDYTDYPKASAVSDLKEQYPDSDIALIGIDMVAYMKKYHSKKVRKNVTIPEWLND
                     AAEDKNLNFSQVLTEALELKLQA"
     gene            complement(7036..7221)
                     /locus_tag="VOX58_000362"
     CDS             complement(7036..7221)
                     /locus_tag="VOX58_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HES5877629.1"
                     /translation="MPMTPKQMIKLLKKNGFYEVSQNGSHKKFRDNNGHQTIVPMHNK
                     DLGKGLEDTILKQAGLK"
     gene            7284..7751
                     /locus_tag="VOX58_000363"
     CDS             7284..7751
                     /locus_tag="VOX58_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HES5877630.1"
                     /translation="MRIKYTKGGDGKLSKLTLKQKRFADEYIISGNAMESAIKAGYSH
                     NYSKAQSHKLLENVGIKEYLDERFEKMDSKTIADQKEVLQYLSSVMRGEQQEKTLISI
                     GELGQEIVDIDVGAKDRLKAAELLGKRYRLFTDKIEAEVQGTVVFVNEDDIPD"
     gene            7729..9036
                     /locus_tag="VOX58_000364"
     CDS             7729..9036
                     /locus_tag="VOX58_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HES5877631.1"
                     /translation="MKTTYQTKPKLEIKVDLPKTIGIGYGAFWRSKNFYRVVKGSRGS
                     KKSKTTALNFIVRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNQLKVTHLFKFNES
                     LPEITVKATGQKILFRGLDDELKITSITVDVGLLCWAWFEEAYQIETEDKFSTVVESI
                     RGSLDAPDFFKQITVTFNPWSERHWLKRVFFDEETKRADTFSGTTTFRVNEWLDDVDK
                     RRYEDLYKTNPRRARIVCDGEWGVAEGLVFDNFEVVDFDVEKTIQRVKETSAGMDFGF
                     TQDPTTLICVAVDLVNKELWLYNEHYQKAMLTDHIVKMIRDKNMHKSYIAADSAEKRL
                     IAEIKSKGVSGIVASLKGKGSIMQGVQFMQGFKIYIHPSCEHTIEEFNTYTFKQDKEG
                     NWLNEPIDKNNHVIDAIRYALEKYHIRSNESNQFEVLRAGFGY"
     gene            9048..10550
                     /locus_tag="VOX58_000365"
     CDS             9048..10550
                     /locus_tag="VOX58_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HES5877632.1"
                     /translation="MYTESFRDSTGKTKTLEFRFHREARMRYQAESLESLLTEKYKLL
                     REMIEHHDKVQKPRIQELLDYAEGNNHTISEIGRRKDDDMADVRAVHNYGKYISTLKQ
                     GYLVGNPIRVEYIDGTEQQQDLLKDLSVKNNFHQLNRRLVKDLSKVGRAFELIYRSMD
                     DKTEVVRLDPREVFVIYQNNLEQSSLAGVRYYNKNQLDGTTKIVELYTDNKILKFEYD
                     GDLTLIREASSHAFGSVPITEYLNTDDGMGDYETELSLIDLYDAAQSDTANYMQDLSD
                     AILAIFGRVSFPHDVQTAEQRIEFVKVMRKARLLNLEPPVDQDGREGSVDAKYLYKQY
                     DVQGTEAYKNRIVSDIHKFTNTPDMTDSKFAGQQSGEALKWKVFGLDQERVDMQALFE
                     QSLKRRYKLIARVSQLLKEIDDFDISKLKITFTPNLPKSLQEKIEAFKALGGELSQET
                     AMAITDIVEDAKKEISLINSESKSRSQLAQKLEETSRLTDRELAHDHQKE"
     gene            10531..12093
                     /locus_tag="VOX58_000366"
     CDS             10531..12093
                     /locus_tag="VOX58_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HES5877633.1"
                     /translation="MTTRKSKYWRDRIKKEMDAKEADDISLEQSMKQLHDYHFRNIEK
                     EIESFYQRYADKEKIDLSEARKRASELDISAYQKKAKELIAKAEKLRKEGRTVTKADF
                     THQENADMAIYNLAMKTNALELLRLNIDLEMQELANGEHKLTKKFLDEGYRKEVEFQA
                     GLLGLSVASQANVKNLADAVINANFKGAKWSDNIWDRQDKLRSIISQSVQSAILKGKN
                     GLTIARDIRREFDVSASYAKRLAITEHARVQMEVGRLSMAENGFAMFDILPEPKACDV
                     CKDIAKHGPYHLDKWRIGENSPPFHPYCRCAVVGLDKKQEEKVNDKSEKFENITPNLM
                     PLFSKFASKITDLQRKIVYSADLADTGYIRTPHAFDINNTLRNKGYNYLNVDDKLITD
                     TLDSVISINSTPKNIKVYRFDDFELLGSINEQNNNIFDSGNFMDKLNQGGLSYTNDGY
                     TSASYDVKKNVMGYRPIKTEIKVPKGSHVYLTDNEEESEIILPRGTKYDIINAKINEY
                     EEIEITMEIRKE"
     gene            12097..12282
                     /locus_tag="VOX58_000367"
     CDS             12097..12282
                     /locus_tag="VOX58_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877634.1"
                     /translation="MDFSDFLNKKQKEWDESHPIPDFSAMSDEELLYQPMSEALVSER
                     FAKELSKEVRKRNLLSK"
     gene            12352..12666
                     /locus_tag="VOX58_000368"
     CDS             12352..12666
                     /locus_tag="VOX58_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877635.1"
                     /translation="MNRESKPGMESVKIGGLTYSVEKASDLQGKDGNWGLIHYKTQQI
                     KLDDSLTEQLEDQTLIHEIVHGILVEAGYTNHEEDQANRIGLILYQVLQDNDFSWLYK
                     GE"
     gene            12669..12935
                     /locus_tag="VOX58_000369"
     CDS             12669..12935
                     /locus_tag="VOX58_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877636.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAIQAKALRNQNKQIAELRAIIQ
                     QNVQAINREFVTAKNTILDNQLAIKLIGDDVGHIKQNYKRKWRK"
     gene            13079..13612
                     /locus_tag="VOX58_000370"
     CDS             13079..13612
                     /locus_tag="VOX58_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HES5877637.1"
                     /translation="MAEETQVVEMVEEQVVPEAKQPQDEKKYTDADVDAIIDKKFAKW
                     KSEQEAEKSEAKKMAKMNEKEKADYEKQKLLDELQELKNDKTRNELTAVARQMFAESE
                     INVNDDVLGLVVTLDAEQTKANVTTLANAFAKVIADDRKALVRQTTPSTGGGLRKQTN
                     YGANLASKAAQQSTKLF"
     gene            13622..14002
                     /locus_tag="VOX58_000371"
     CDS             13622..14002
                     /locus_tag="VOX58_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HES5877638.1"
                     /translation="MNKRKVTTSKEILHNLDYEAISVTLDSKKIGKEVVPAGTVLAGV
                     SGSVFEDRKQKVKAVDNSAVESEDNICGILLTDVDLTNGDAVGSCIYRGTINADKLAD
                     SSIAGKYEVLEEELPHIVFIKGGK"
     gene            14005..15087
                     /locus_tag="VOX58_000372"
     CDS             14005..15087
                     /locus_tag="VOX58_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HES5877639.1"
                     /translation="MALIHEIITSENIKGFYNAKNENVGNTLGENAFPPKQQLGLKLS
                     FIKGAAGKPVTLKAAAFDTKVPLRDRMTVELLDEEMPFFKEAMVVKEADRQQLSLLAQ
                     TKNQELIDTALAAIYNDKVTLIAGAKARLEAMRMEVLSKGKIHVASNGVMKDFDYGLD
                     SSQTTKPDTKWEQVDTATPLKDIEKAIEVMAERGFVPEAIIMNSKTLSLIKNASNTLD
                     VVKPMAPDGAAVTKGDLETYIADELGLKILLKDGMFVGDDGKAKKYFPDGFATLVPNG
                     NLGYTVFGTTPEQSDLLGGEATDAEVSIVETGIAITTTKTTDPVNVQTKVSMIALPSF
                     ERLEEVQIINATESEEETKKENSFEM"
     gene            15097..15339
                     /locus_tag="VOX58_000373"
     CDS             15097..15339
                     /locus_tag="VOX58_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877640.1"
                     /translation="MPRVIRAFKDKVTKVVYEVGDIYSGDRVEFLTEGGVLEPSVDFD
                     KLKVSEIKSKLDELSIEYDAKLKKSELLKLLKQTIG"
     gene            15353..15706
                     /locus_tag="VOX58_000374"
     CDS             15353..15706
                     /locus_tag="VOX58_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HES5877641.1"
                     /translation="MDAVNTSSVISNVKLDLGILDSQQDDLLNMLLKRVTDHFKAKYG
                     AVEIDSAFSFVLEDCLIARFNRRGAERAKSESVEGHTTTYYDFLDEFEPYDAMIMAKL
                     NLIKDKSRKGGLYFL"
     gene            15703..16011
                     /locus_tag="VOX58_000375"
     CDS             15703..16011
                     /locus_tag="VOX58_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877642.1"
                     /translation="MRYADRVTFVKTTDEQYNPDLGEYTHTEVISITKPCFVMDMGME
                     KSVQIFGDYQKDRKVIYLKQPYTKAFDYCEYEGKRYKAQANKLGAIVFYLEGDDSIGG
                     "
     gene            15992..16357
                     /locus_tag="VOX58_000376"
     CDS             15992..16357
                     /locus_tag="VOX58_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HES5877643.1"
                     /translation="MILLVADISLKVVGTAGLKKKLELIVKKDAVKKIVRDNGTQLQR
                     KMINKAVFTKGYSTGATRRSITMQIGDGGLSVKVKPGTHYAGYLERGTRLMSKQPFVL
                     PALKEQKVKFRKDLEALVK"
     gene            16354..16743
                     /locus_tag="VOX58_000377"
     CDS             16354..16743
                     /locus_tag="VOX58_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HES5877644.1"
                     /translation="MIKTRDQSIFDEMFKRIQSLGFKVYDYKPMTEVPYPFVEMESTD
                     AEYIPNKDDIKGSVELMLSVWGVQKKRKQVSDMASAIFSQAMSVARTDGFCWSFNIRQ
                     SSIQMLDDTTTVTPLKRAIVTLRFNLR"
     gene            16798..17421
                     /locus_tag="VOX58_000378"
     CDS             16798..17421
                     /locus_tag="VOX58_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HES5877645.1"
                     /translation="MSKAQEQTKQLEAKQGIHSILLFRLLKEASSEVATKLAFQTEHE
                     VGKSRDVDGQKTKDGIIQSVGALEYDFKATSILAKGDELAAKLEKAMEDGELVEIWDI
                     DSEEISKNGDSDNKLAKVWGINKNGTNGGNGKCLATYYQGYISSFSAKKNAEENIVIE
                     MEFAINGVGKKGFATLTDAQKAAVQYAFKDTTKSNSEETKKENSFEM"
     gene            17475..17828
                     /locus_tag="VOX58_000379"
     CDS             17475..17828
                     /locus_tag="VOX58_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HES5877646.1"
                     /translation="MQLEIKGKTHNVKFGTRFVAEMDKNHIAERQGFKFGAGLQSSVP
                     FLIDHSVVTLAEVIYTGTITEPPRPSLNDIYDYIDEVEDIEKLFDDVLDELRQSNASK
                     LFMAQVEKDLAEAKA"
     gene            17903..18199
                     /locus_tag="VOX58_000380"
     CDS             17903..18199
                     /locus_tag="VOX58_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877647.1"
                     /translation="MTDINEIGRLTLYEYDLLMTGKALAAVDESHKAHKQAWINHQVT
                     ATKLVGGKKNKKEVPVYKKFKDFFDYEEEIRKITQEIDEGYDKKGMDLLLKANL"
     gene            18214..21849
                     /locus_tag="VOX58_000381"
     CDS             18214..21849
                     /locus_tag="VOX58_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HES5877648.1"
                     /translation="MGESYSVEAVLTAVDKTFGKTLQSAIRSIEGLEKRSTGFSSVSQ
                     KASSMFKSMLGANLAGQAISAMTRTVSSGLGSMLGEMNSSAKAWKTFDANLADIGFGK
                     KQILAAKTAMQDYATKTIYSASDMASTYAQLAAVGVKDTGKLVKAFGGLAASAENPKQ
                     AMKSISQQMTQAVGRPTVAWQDFRIMLEQAPAGMAKVAKSMGKNLDELVADIQAGRVK
                     TSDFLEAVKKAGNDKSFQKMATEFKTVDQAIDGMREGLSNKLQPAFEKVNQFGIRAIE
                     AIGKQLDKVDFSKFASNLGKFLEGINIDKIVSNISSAISSVTSKVKEFWDGFKQTGAI
                     SAFSGALQSVWEALKNVASAMSGGNWKTFGATVGGTVKHVSNFAKAVSDVLGKMDPGR
                     LRSWIATFAAVAGGFKLFEKLTGQSVIGSFLDKIGSKFGLFGNKAKEGTDKASNGARR
                     SGGIISQIFSGLGNIVKSAGTAISTAAKGIGAGIKTALSGIPPIISSLGTAISTVAQG
                     IGTGLAIAFKGLGSAIAMVPPTTWLALGAAILMVGAAFALAGTQADGISQILRTVGDV
                     VVQILQQVTDSLATLLPIIANAIGSMLPIVAGAISQIVGAVAGGLSQLVIAVSTGASL
                     VIGAFTGLLGGISGVINSISAVIQSLTGVITAVFNGIATVISSVGSAIKDVLTGLGTA
                     FEGFGNGVKSALEGVGAVIESFGSAVRNVLDGVANILDSMGTAALNAGRGVKEMAKGI
                     KMLVDLSLGDLVATLAAVASGLGKMASSAGEMTTLGSAMSKVANGMTRLATSATIAIT
                     GLTVFATTMATIKTAVATLPPVLTMAASGFTTFTTQAVAAVTGLAAINAPITMFKAQL
                     MTITPALAQAGAGFAAFVAQSSTFSTGLASAGPTIAAFNANLMSLSATTGALVASIAG
                     LSAVLSVVSAGFSQIGASATATVGQIQAFASSTTVVSSAFASMQSMIQSAMAAIVSSI
                     ITSFNQAASQMQSILSRMLSQARTFGSQLEQQMRQSGQRSGQNLARGLSSQQSAVINA
                     ISSMVNAAVSRANAGAGPMRQAGAYIGQGLAQGMYSALGAVTAAANALVAQAERAARA
                     KAMIHSPSRLFAKRVGQYIPQGVAMGIDKNADVVDDSVGGLFDSINSFDFNIADRLAS
                     IGAKFQGVIKSESSQSLSQQQEFVHTAQPAYINFSLGGNEYEAFVSDITNQQAKIEKI
                     RLKRSSW"
     gene            21881..22660
                     /locus_tag="VOX58_000382"
     CDS             21881..22660
                     /locus_tag="VOX58_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HES5877649.1"
                     /translation="MYEFNDTIRGTPKVTFNLKTTIGEKVLEEELNNDLGTFRTLTVS
                     GRDVVDLEHQTTSVLGRNGEYFHNATVEVRKLEIKAKITGKDNQSMRLQYEKLNRLIV
                     SHNQVFLSFSDEPDRNYLGIFKSKDVPEEVSNEQIIGLTFICYNPFKMSDVKTKKGTS
                     IQNGGLFQTKPIITLNLSSPTKEIKLLHVESQKYIRLTGTYTTDEIKIDMATGKITQN
                     GRNILGDLDMINSRYFELLPGNNTLQCANAAITAEFREVYL"
     gene            22657..24711
                     /locus_tag="VOX58_000383"
     CDS             22657..24711
                     /locus_tag="VOX58_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HES5877650.1"
                     /translation="MIYLFDKLERLVATVGTDDLLSWHFKVKNNDWDQASFEVPVDYD
                     VEPFVYFGFFNYDPNQKEDVFKLFKVIDYNLEDSKFYKGLDKAESDLDTIAIIKDKRF
                     RQSSADACIDGALEGTGYQVGKVEGITNVRTLSYYYISPRAALIKIVEAFNCEFNVRY
                     TFINNKITSRYIDLKKRFGKPTGKQFEHGNNLLKVVYEESTDDIVTCLIGRGKGEEIQ
                     HEEAEPKDVEGHLPQEERRQGYGRRIEFTDVVWSVEKGDPIDKPAGQNFVALDSAREE
                     YGLSQNGELKHRWGVFVNEEIEDKTELLKATWEELQRLSIPIRIYKAEILDIGPETWK
                     GDSVAIIYDEVKIAFETRVDEIDIDKLNFNRSVVTLGDYSVVQNRESRSRKEAVQNMI
                     DESLETITDLGMTFQEFLQGIEKRIETGKKEMEDNWRKVNLEFDNFKKKVEQEGLQFN
                     TLKEQIKEVDERTDKELEEFRATLKNLALPEEAIKKITEAIKVDDIPSLKQNFDDLKN
                     KVSETSETARLNAEIIGTDGKTRYNKNLLVGDPNRVKTYDQDYIEVEANDGGFKRGET
                     YTISFSQTCELLKKVAITLTQINNKGVKLVLTPTKAKMDAQTFEVTKDKQSIEVYPLS
                     YTGVLTGDWYKSKSVEINASEAQELALEMAYKEIADAKGATIIGKQSDKPKIILDGRR
                     DR"
     gene            24708..25922
                     /locus_tag="VOX58_000384"
     CDS             24708..25922
                     /locus_tag="VOX58_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877651.1"
                     /translation="MTSVETIPIKIVFDRKDASEWQSTNPVIDEGELVVELDTHKLKV
                     GDGKTSYNDLPYYEGPQGESITKVQLSENGDLSVWIGDKETKLGNIKGQKGDKGTSIT
                     DITKDGETLTIKLSDDTQKIFNIPNGQKGDRGKGVESARIDESGHLKLKIEEESELDL
                     GNVKGESGPKGDSITITNHKRVSDGTQVSFSDGTQIVVPKGDTGDVNGINLEDYVKKS
                     ELKNVGSADVKAINDFLGLSQKVFTSSYSYTDSLLKSYAKPSYSASWYVNESTVSTKN
                     GDKVLITIHNTTTQADNYLEVAVTYVGANYVTATSTGRLLTTPGEVKVVTKKQAEKDY
                     AAKKHKHEISDIAGLNERLSGYLRQADIQSQLNNIGKLKDTQTGQYLEVKVVDKGQVP
                     SNTSGMIVFERS"
     gene            25909..26238
                     /locus_tag="VOX58_000385"
     CDS             25909..26238
                     /locus_tag="VOX58_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877652.1"
                     /translation="MRGHNLLKDLNNIIINNKTIDRIICNNTIVYQRSWDLLFDGLLT
                     SAGKKLDIYPFYKIVINNAFFVKTPTTKNNTIFLAGHKILALQDSHFCFFRALEKSFF
                     VKIYGKN"
     gene            26249..28144
                     /locus_tag="VOX58_000386"
     CDS             26249..28144
                     /locus_tag="VOX58_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HES5877653.1"
                     /translation="MSRDPTLLIDESNLTIGSDGRAYYTFTADGDTKSVKIANNKCIG
                     TTRFNQLMIERGGKPTNYVAPVVVEGTGNPTGLFKDLKEISLELTDTKNSKLWSKIKL
                     NNQGMIEEYYNGTIKSEIIKTAEGTQQRISSETDKKLALINETVSGIRREYQDADRQL
                     SSSYQAGIEGLKATMANDKIGLQAEIQTTAQGLYQRYDNEIRKLSAKITTTSSGTTEA
                     YESKLDGLRAEFTHSNQGMRVELESKISGLQSTQQATARQISQEISNREGAVSRVQQG
                     LDSYQRRLQNAEGSYSSLQQTVSGLQSDVNSPNSKLNSRISQLASQIDQRVTRADVTS
                     IINQSGDSIKLAIQRAGGIDAKMSAKEIVSAINLNGYGVRISGERIALDGNTTVNGAF
                     GAKIGEFIKLKADQIIGGTIDANKINVINLNASSIVGLDANFIKARIEYAITSLLEGK
                     VIKARNGAMTIDLQSGQINHYTNESAMRRIDSSTASQFIKMTKSGFISEIGNMQAAMT
                     VIGSNSDGSENHENKTFGGIRIWNGKSSYQSTSFVELVGNRVAIYGNKNRSPWLFDST
                     TSGYAYLIPQNESGIKHAIGRADRKIDQIHVGDIYVQGERVAMMLKDLANRIGYIGTG
                     GWANRIG"
     gene            28158..28319
                     /locus_tag="VOX58_000387"
     CDS             28158..28319
                     /locus_tag="VOX58_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877654.1"
                     /translation="MQEKLLGKIINDLTLKVANLTLENAQLKAQHEIELEELNAQLDE
                     ATAPKEEGK"
     gene            28322..28939
                     /locus_tag="VOX58_000388"
     CDS             28322..28939
                     /locus_tag="VOX58_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877655.1"
                     /translation="MRNWKVTGNYPQWDGTGAVIDTQVIITDDRGAVIAEKIKKDLRT
                     ANDAEIIEAALEEFKKTAYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQKMINLQTIHMLTSGGKIDPDIYKGMLELIEPAKKGEYQAYDVFTVVDSTKEED
                     GEAGEGNLVFVHVNEPFTYEAQTLKDLESEDKVTVIKYADLVKQD"
     gene            28950..29246
                     /locus_tag="VOX58_000389"
     CDS             28950..29246
                     /locus_tag="VOX58_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877656.1"
                     /translation="MIDFVQIGAFCGAALSILGVWGFIVNPFKKAMEANEFAMAQLKD
                     SIKELAYELKNLDREREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            29243..29428
                     /locus_tag="VOX58_000390"
     CDS             29243..29428
                     /locus_tag="VOX58_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877657.1"
                     /translation="MNNWFKKVAIKTIKTMAQTAVGLIGSSMLITDINWPTMLSAVLL
                     SGLTCILMNVSQIKEEE"
     gene            29540..30874
                     /locus_tag="VOX58_000391"
     CDS             29540..30874
                     /locus_tag="VOX58_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HES5877658.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSLGAQCWDGYADYCKYLGLPYANC
                     TNTGYARDIWEQRHKNGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGSG
                     YGWFLGQNQGGANGAYNLVKIPYSTTYPTAFRPKVFKNAVTVIGNIGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSNL
                     AQREADLFLSNLPTKKVSYLVIDYEDSASADKEANTNAVIAFMDKIANAGYKPVYYSY
                     KPFTLNNIDYQQIIAKYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQSQLTFNQKLDTNTKLDNSNVPYYEATLRTDYYV
                     ESKPNASSADKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLSNATQV"
     gene            complement(30950..31657)
                     /gene="speC"
                     /locus_tag="VOX58_000392"
     CDS             complement(30950..31657)
                     /gene="speC"
                     /locus_tag="VOX58_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HES5877659.1"
                     /translation="MKKINIIKIVFIITVILISTISPIIKSDSKKDISNVKSDLLYAY
                     TITPYDYKDCRVNFSTTHTLNIDTQKYRGKDYYISSEMSYEASQKFKRDDHVDVFGLF
                     YILNSHTGEYIYGGITPAQNNKVNHKLLGNLFISGESQQNLNNKIILEKDIVTFQEID
                     FKIRKYLMDNYKIYDATSPYVSGRIEIGTKDGKHEQIDLFDSPNEGTRSDIFAKYKDN
                     RIINMKNFSHFDIYLEK"
     gene            complement(31768..32526)
                     /gene="mf2"
                     /locus_tag="VOX58_000393"
     CDS             complement(31768..32526)
                     /gene="mf2"
                     /locus_tag="VOX58_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HES5877660.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAAKVRTYPNVSHANTH
                     YKNTASSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVTMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGTATPKN"
     gene            32766..32954
                     /locus_tag="VOX58_000394"
     CDS             32766..32954
                     /locus_tag="VOX58_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HES5877661.1"
                     /translation="MLTYDEFKQAIDNGYITGDTVIIVRKNGQIFDYVLPGEKVRPWE
                     VVTEEVVEEVMVELDYIK"
     gene            complement(33106..33453)
                     /gene="ssrA"
                     /locus_tag="VOX58_000395"
     tmRNA           complement(33106..33453)
                     /gene="ssrA"
                     /locus_tag="VOX58_000395"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            33545..34159
                     /locus_tag="VOX58_000396"
     CDS             33545..34159
                     /locus_tag="VOX58_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HES5877662.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            complement(34286..>34896)
                     /locus_tag="VOX58_000397"
     CDS             complement(34286..>34896)
                     /locus_tag="VOX58_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5877663.1"
                     /translation="GLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSHQ
                     IILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKMDYFINMLTVLA
                     YVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHSHIFNINT
                     SADSFPFTEQKTIYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
BASE COUNT        11938 a         5785 c         7460 g         9713 t
ORIGIN      
        1 gctatctata tcaacgtttt agaggttacg tagtaaaaaa gggggtgcgt taccttatgt
       61 accacacaac agcactttca ttcttaaaaa aaggatatca ggttataccg cttagaaaag
      121 atactggaac gccaatgatt aagtttaaag atatccaaat aacggaagaa gtgatcaaaa
      181 atacaaactg gtttaattgt gattatgctt tattaatgcg tggcatttgg tgtattgata
      241 ttgatactca tgacatggac gagaagttag ctaaagagtt atacataatg ataaaaaaga
      301 tggggattga tttattatct gtattatcga ctgataagta tgacaatgga ctagatggtt
      361 attcgtcaat tattaggcat gagtataaaa acgaattaat cagtaatttt aaaaatacat
      421 tcgcagaatt aactgcaagt ggtggtatgc acatactctt taaaaaacgc gatggtatta
      481 attacacgca aaaaatagga gtaatgcctg gtgttgacat aaaagcgaat gataataatt
      541 ttgtcaaaat attcccatct gacggacgtg aagttttaca agcagttaaa acattacctt
      601 attatgatgg aaaatttgaa gaggaagcat ttaaaccaaa acaagaaggt ataacaacct
      661 attttggagg ttctgtcaca tatacatcaa acggaagtca cgaaggccga gaggcttatg
      721 aacgtgtagc aactggaaca tcatataaca ggaataatga tttgtttaaa ggagcgtgtt
      781 gggcgtttga aaatggtatc gatattgatg atttaacatc aattattggg acggttaaag
      841 gcagagacgt atttacgaga gaggagtttg aattaacgat tgaatcagcg aaacgaaaag
      901 tcagctacgt cactatcaga acatgatatc caaaacctta tccgaatgga attatcacaa
      961 gcggggcata tggtatttcg agcaaatgtc ggaaaggtga aaacagcaga tggtagattt
     1021 tttgatacag gtttaccaaa aggtttttgt gatttgtttg gatttaaacc aaacggacaa
     1081 atatttttta tcgaagttaa aaatgaaaca ggtcggataa gaccagaaca gaaaaaattt
     1141 atggaggtca tggcatctag aggagcgctt gtaggagtgg ctcggtcagt ggaagatgcc
     1201 ttaaacatag tcaatgacac tagtagatga tttttacaaa caaatggagc cgtcaattaa
     1261 agcgttttta gacgataaca ttaccatcgc agacaaagaa gaagctgaca gagtctatag
     1321 atctgtcaaa tactataaaa aactaaacag attgccgcca cctgatgtat tggagtggtt
     1381 ccaacgaatt tacacgacaa aggaaatgat aatgttaatc aagcagtctt accgccttaa
     1441 acaaaaaaag acagatgagg atgacaagat ttacgaaaag tggatgttta aaaactacgg
     1501 tgacgttaag ctcgttaaaa aaatcaaacg cacggacgca ctagaaagag ctcggagaat
     1561 gggtctatga aaagacacag acagtggcat aacgatatta aatatacacc tagatcttac
     1621 gataatctgt tgccttacga tatatcagag ctgttaatag ctcacagatg caaaataaag
     1681 atgtctgatg acgttttagc agataagata ggcatttata cttggcaatt aaaagcgctc
     1741 ttagaacgca gaatattgcc aaatgagagc gagtgtaaat tgattataaa ttttttgaga
     1801 gaggtggagt gatgatagaa gaattagtac ataaaccaaa gcattataat cacggagaaa
     1861 ttgatttgat tgagtcgtgg tataagacgt acccatggga ccagtttgtc gcaatcatgg
     1921 aaagtcacat tgataaatac attaaacgac atagatacaa aggcaatgcg actcaggatt
     1981 tagaaaaagc actcgaatat acaaagcgtc tcattgaata ttggttaatc gaaagcgtgg
     2041 aagaatgaag agatatattg aagttaagga cgaatggaaa agtgcaacag accacctgaa
     2101 cgattttatc gacaaaaaca agtacgcaaa agtgacagtt gttggttatc aagtcgtaca
     2161 actttctcct tacggaagag atttgactta tattttggca gaggtggaag aatgacaata
     2221 gatgaagcgt tgcaaaattt acgtgataac tttaataaaa taatgaatgt cctaaaaaac
     2281 gattggaaag cactattgtt tcttgcaatc gcaatatttg ggatgatggt aaccgtgtcg
     2341 tattttagct atcgcgacgc acgacaatat tacgagtcgc aaatcacagg actacgtaca
     2401 cagctaagca ggacacaaaa gcagcttaaa cgtgctagcg aagatagagc tagacagaca
     2461 aagcgaattg cggaatttac gcacaacgga gggtaacatg attaagatcg atgagataca
     2521 tcgcatacta ggcatcgacg aagtttataa agcacccaaa cgacttacgg acatactctt
     2581 tgataaagat agtcgtgagg atatatttag acagtttttg aaatatgaaa cagatgtatc
     2641 ttacgactgg tttatgcaat attttgagga agaacaagct gaccgcaaaa ataaaaagca
     2701 agactttacg cctaaatcgg ttagtacact attatctaaa ataataagtg gtaatcaata
     2761 ctacgaggta gcagtcggga cgggtgggat acttatccaa gcatggcaag aacaacgatt
     2821 aaatgacagt ccatttactt atcgtccgag taaatactgg tatcacgtag aagagttatc
     2881 ggacaaagca gtaccgtttc tactctttaa tatgtctata agagggataa atggtgtggt
     2941 ggtgcatggt gactctttaa caagacaagt taaaaacatt tattttttgc aaaatacaaa
     3001 agacgacatg ctgagtttta gtgatattaa cgttatgcca aggactcaag atattgagca
     3061 agaatttaat gtcaaagagt ggattggtga tgcgatagtg catgtagaga gtaagttaaa
     3121 tgcgaggtaa caaaatgaac attgaagaag ctattaaaaa gataagagaa ctagaggcgt
     3181 ttaattattt cgactttaaa ggtaatttaa ttagaagaat tgatgtatta ggtatcatcg
     3241 accaactaaa aattatagta caacaatgtg ttgcagattg gtacgaaaag cataaagata
     3301 atttggaata tgatttatat ttatatcaaa tgtcaatcta tgatgaaaaa gttgaagaag
     3361 atgattttta ttattggatg caaaaatcaa agaatccagt tcgtactctt attaatatgc
     3421 accaatttgg ctatgaaatc gagcaagagc agctgtatac agttgatttg ccaaacggtc
     3481 agcctttagt acgcggtaaa aacactttgt attttagtca aaatctaaca gccgaaaatg
     3541 caaaactcac tgaatcagaa atccgaaaag acttcgagtg ggcttggcaa tttgcgaaag
     3601 aggtgactga atgactgaag agttaggagt gttatatagc gaaaaatggc ataagtatta
     3661 tttatataaa acttgcaggt atatatctta tgttgataat ccacatcagg ctactaaatg
     3721 cacccgcaaa caagcagaac agtttccaca gtttaaatgg gtatcgctga caaaattata
     3781 accccacgca agcgctcaag agcctgcgat ggctctgtgg gggtggaccg aaattaaaaa
     3841 atagaaacga gaacctcctt acaccaaaac aaatctaaag cgggttatcg gtcatccgtg
     3901 attatccaag gcgtcgctaa tgctttaaca cgacatcgtg cgcctgtgtc aaaaaacaaa
     3961 ggaaagagag ggcttttctc cacaaaacaa aaagacgtcc acacggaacg cccccttggt
     4021 taaatttaag cttaaataaa ttataccaca tcgggggctt tcatgacgtt ttttccagaa
     4081 attaatatac aaaaaactaa atcaaacgct aagcgaaaac taagagagta tccacgctgg
     4141 cgaaggatag ctaatgacgt agatactcaa aaagttacag ctacttactc atttgagcca
     4201 aggcaaccgc atggggtccc tagtaaacca gttgagagac tagcgctaaa ccgtgtgtca
     4261 gcagaacaag agctggatgc gattgagcaa gccgtcagta tgatactaga gccagagaga
     4321 cgcaggattt tgtatgacaa atatttagcg ccttataaaa aggcagataa ggttatttat
     4381 acagaattgt gtatgtcaga gagcttttac tatgatacac ttgacattgc tttgttagct
     4441 tttgcagagc tgtacagaga gggtgtgttg cttgttgagc aaggagtttt tagctagttt
     4501 ttatacagta ataagatagt ttatacatat ttttacatgt tattatagta ctatcaaaat
     4561 aacaagaaga gataaccttt taatcactga ctattttatt tagtcgccaa ctttaactac
     4621 gatcaaactt gttattttat ggtatgtggg acgtgcaggt tcgaatcctg ccgtctctat
     4681 cagcgtcagg ggagacgcga ccccacaccc atttaaaggt agacacgatc tgatgtgtgg
     4741 ggttttatga ttaaccgcaa gtaaaacaag ggtcgcaacc ttgcttgtgg ttagtaggct
     4801 taatggtcgt ttatgcacgt tcgattcgtg caaagcctgt taggaacatg aaccgtgatt
     4861 ggaaaacggt ggaggtagcg ccttgcttcg ggacgttagc taagtccgaa aactctttgc
     4921 gaagagccag aagaagatgt gtcggttcga ttccgactgt tcctgttgca tttaatgcaa
     4981 actccatatt tttattaaaa aagcctatta tacctattgt gtaagggctt ttttcgttgt
     5041 attattgaag tgataaaaaa tatggagatt aaatatggaa aacaaacccg caaaagcaag
     5101 tttctttaaa ttaagttctt ctaaagaatc taatttaaaa ttgtttatcg aaaagatgga
     5161 ggaaatgttt gataatttta gaaaaaaaca atatagtaac attccaacat tagagataaa
     5221 tgatcttatg tattatataa atgcaatgca gaaggtaaca agtgaagaag aattaaatgg
     5281 aacgaatcta ttttattggt tagttaccat ttcacgggtg gatacagaat ctccaattat
     5341 tcttgcgaat cttgaaaaaa atattgatgt aagaaaaagg gaaattgaac atggggataa
     5401 tgagggcctt gtggttgata caagattatt atttgaccca tttagacaaa ttttagttgt
     5461 ttataatcaa agaggtacaa ttaataatta cgacttaaga aggttttttt gtcaaataat
     5521 tggagttaga ggattgaagt ttgatattat attaaacagt gatgctttca aaagggttgg
     5581 taagcttgat gttgtaaatt caattagtta tactgttgca agcccaacaa actttaagga
     5641 gtttcgagat gatactcaaa gtgaaaatgc cgatttaaag tttgcaaatt ctatgctagg
     5701 agagtcaatg caggttgtta taaagtcaaa ccatctttct aaaaaaaata tatttgataa
     5761 attttcagat atgttagtta atgactcagt taatgtaaaa aatgctaaag ttgaagggct
     5821 taccgatggt catccggaat taatagactt aataaaaaat aaattggaat ataagggaac
     5881 tattttttat gaaaatactt tagacgatga ggctgtttat gcatttctga ataccgcgta
     5941 tagctttcat tttagtcatt taaaaagaat gtttactata actttataac ttatattgag
     6001 gtatgcgatg aaaaaaggaa ggtttaaaag aaactggccg attatactat ttggtgtaat
     6061 agtttttata tttagttttt attttaaaat ttctccaaaa agtttaaaaa attttactga
     6121 tataatgtcg gcatctctat cattttcagc aattgtaacc gcaatattct ttgctagttt
     6181 ttcattgata ccaacttccg gttctaataa attagtagtg atgatggaag atttagggac
     6241 agagataaaa ataatggacc gcctattagt tgctactttt ttatctttca ttagttcttt
     6301 attatctttc atttctctat tctttagtaa gactgatact gatttaattt caattttagt
     6361 agtttcaagt tggttgagct caacagtaat gatgttccta agttcgttct ttgtgctaag
     6421 aacattaatt ttgttagttg aaacttataa taatttcaaa aataaatagg tcatcacatt
     6481 gtggtgactt ttttgttatg gtccacgaca tggttactta ttagttacaa acgttgaaat
     6541 tacaatgttt cgataggtca acgtcgtggt tttttattat gtaaaaaaga accacaacag
     6601 tggctcttat gcttgtaatt ttaattcaag cgcttcagta agaacttgag aaaagttgag
     6661 gtttttatct tctgctgcgt cgtttaacca ttcagggata gtcacgtttt tgcggacttt
     6721 tttggagtga tattttttca tataggcaac catatcgatg ccaattaaag caatgtcgga
     6781 atcaggatac tgttctttta aatcagaaac ggcgctagcc tttggatagt cagtatagtc
     6841 ttcaagggca aaacctaaga cttcgacagc catttcataa gcctcttgaa agtcttcacc
     6901 ttgagtaatt gcttcaggta catctggaaa tgtaaccatg atatagtctg tgtcttgtgt
     6961 aaatgtagct ggataaatta acataatgat cctcctttga ttactgtgag ataagcacac
     7021 catctggcaa gcggattatt ttaaacccgc ctgctttaga atggtatctt caagaccttt
     7081 tccgaggtct ttattgtgca ttggaacaat ggtttgatgt ccgttattgt ctcggaattt
     7141 tttgtgactt ccgttttgac tgacttcgta gaacccattc ttttttagca gttttatcat
     7201 ctgctttggg gtcattggca tattgcttac ctcactttct atacttatat tatacacata
     7261 aacaatgtag atgtcaatgc aaaatacgca taaaatacac aaaaggcggt gatggaaaat
     7321 tgagtaaatt aacactaaaa cagaagcggt ttgcagatga atacatcatc tcagggaatg
     7381 ctatggagtc agctataaag gctgggtata gccataatta ttccaaagca cagtcgcaca
     7441 agctattgga aaatgttgga attaaagagt atcttgatga acgatttgaa aaaatggatt
     7501 ccaagacaat agcagaccaa aaagaggtat tgcaatatct ttcttcggtc atgagaggtg
     7561 agcagcaaga aaaaacactc atcagcattg gagagcttgg gcaagaaata gttgatattg
     7621 atgtaggagc aaaagacagg ttgaaagcag ctgaattgct tggtaagcgg tacagattgt
     7681 ttaccgataa gatagaggct gaggtgcaag gaacggtggt gtttgtcaat gaagacgaca
     7741 taccagacta agccaaagct agaaatcaaa gttgatttac ctaaaactat cggtataggt
     7801 tatggcgctt tttggcgctc taaaaatttt tatcgagtag ttaaaggcag ccgtggatct
     7861 aaaaaatcta aaacgactgc tttaaatttt atcgtcagac tgctgaagta cccttgggct
     7921 aacttattgg tcatccgtag atactcaaac actaacaaac aatctactta taccgatttt
     7981 aaatgggcgt gtaatcaatt aaaggttaca caccttttta agtttaatga gagcttgcca
     8041 gagataaccg tcaaggcaac aggacaaaaa atcttattcc gtggtcttga tgatgaactc
     8101 aagataacat ctattactgt tgatgtcggt ttgctctgtt gggcatggtt cgaggaggct
     8161 tatcaaattg agaccgaaga taagttttca acagttgtcg aatcaatccg cggtagttta
     8221 gatgctcctg atttttttaa acaaataaca gtcacgttca acccgtggtc agaaagacat
     8281 tggcttaagc gtgtcttttt tgacgaagaa actaaacggg ccgatacatt ttctgggact
     8341 acaacattta gagtaaacga atggcttgat gatgtcgata aaagacgcta cgaagatttg
     8401 tacaagacta atccaaggcg ggctagaatc gtctgtgatg gtgaatgggg cgttgctgaa
     8461 ggtcttgttt ttgataactt tgaagtcgta gattttgatg ttgaaaaaac aattcaacgt
     8521 gtcaaagaga cttcagctgg tatggacttt ggttttactc aagaccctac aactcttata
     8581 tgtgttgcag ttgacctcgt aaacaaagaa ttatggcttt acaatgaaca ttatcaaaaa
     8641 gctatgttga cagatcatat tgtcaaaatg ataagagata aaaatatgca taaatcttat
     8701 atcgcagctg atagcgctga aaaacgtcta atagcagaga taaaaagcaa aggtgtatct
     8761 ggtattgttg ccagtcttaa gggtaaaggg tctattatgc aaggagttca gtttatgcaa
     8821 ggttttaaaa tatacatcca cccttcttgt gaacacacaa tagaagagtt taatacttac
     8881 acttttaagc aagacaaaga aggtaattgg ttaaacgaac cgatagataa gaataaccac
     8941 gttattgatg cgattagata tgcgcttgaa aaataccata tcagaagcaa cgagtcaaat
     9001 cagtttgaag ttcttagggc tggttttggt tactagaaag gaaaataatg tacacagaat
     9061 catttagaga tagtacggga aagactaaaa cattagagtt taggttccac cgtgaagctc
     9121 gcatgaggta tcaagcggaa agtctagaaa gcttgttaac cgaaaaatat aagctactcc
     9181 gtgaaatgat tgaacaccac gataaagtcc aaaaaccacg catacaagag cttctagatt
     9241 atgcagaggg aaataaccac accatcagcg aaataggccg taggaaagac gatgacatgg
     9301 ctgatgttcg tgctgtgcat aactatggta agtatatttc aacgctcaaa cagggctatt
     9361 tggtgggtaa tcctattcgt gtagagtata ttgatggtac cgagcagcag caagacctat
     9421 taaaggacct atctgttaaa aacaatttcc accagctgaa ccgcagatta gtaaaagacc
     9481 tatccaaggt tggtcgagcg tttgaattga tttatcgcag catggatgac aagacagagg
     9541 tcgttagact agatccacgg gaagtatttg ttatctatca aaataaccta gagcaatcaa
     9601 gcttagctgg tgtgcggtac tataacaaaa atcaattaga tggtactaca aaaattgtcg
     9661 agctttacac cgataataaa atcctgaagt ttgaatatga tggtgattta acactgatta
     9721 gggaggcttc gtctcatgcg tttggttcgg taccaatcac ggagtatctc aacacagatg
     9781 acggcatggg cgactacgag acagagttgt ctttaatcga cttgtatgat gcagctcagt
     9841 ctgatacagc taactacatg caagatttgt cagacgcaat tctagccatt ttcggacgtg
     9901 tgtcatttcc gcatgatgtt cagacagccg aacagcgtat cgaatttgtg aaagtgatgc
     9961 gtaaagctag attgcttaac ctcgagcctc ctgtcgacca agacgggcgt gagggatctg
    10021 tagatgccaa atatctatat aaacaatatg acgtacaagg aaccgaagcc tataaaaatc
    10081 gtattgtgtc cgacatccat aaatttacca atacgccaga catgacagac agtaagtttg
    10141 ccggtcaaca atccggagag gcgttgaagt ggaaagtgtt tggtcttgat caggagcgtg
    10201 tcgacatgca agctttattt gagcaatctc ttaaacgtag gtacaaacta atcgctcgtg
    10261 taagccaact gcttaaagag attgatgact ttgacatcag caagcttaaa atcacattta
    10321 cgccaaacct acctaagtcg ctacaagaaa agattgaagc ctttaaagca ttgggtggag
    10381 agttgtcgca agagacagct atggctatta cagacatcgt ggaagatgct aagaaagaaa
    10441 ttagccttat caatagcgag tcgaaatcac gtagtcaact agcgcagaag ttagaagaaa
    10501 ccagtagatt gactgatagg gagttagctc atgaccacca gaaagagtaa atactggcgt
    10561 gaccgtatca agaaagaaat ggatgctaaa gaggcagacg atatctctct tgagcaatcc
    10621 atgaagcaat tgcacgatta tcatttcagg aatatcgaaa aagaaattga gtcgttttat
    10681 caacgttatg ctgacaaaga gaaaatagac ctttcagaag cccgtaagag agcttctgag
    10741 cttgatattt ctgcttacca gaagaaagct aaggaactta ttgcaaaggc tgagaagcta
    10801 cgaaaagagg gtagaacggt aacaaaagcc gacttcactc atcaagaaaa tgcagacatg
    10861 gctatttaca acttagccat gaaaaccaac gctttagaat tgttgcgctt gaatatcgac
    10921 ttggagatgc aagaacttgc caacggcgaa cacaagctaa ccaagaaatt tcttgacgaa
    10981 ggttaccgta aagaggttga gttccaagct gggctattag gattatcagt tgctagccaa
    11041 gcgaatgtga aaaacttagc tgatgccgtt attaatgcta atttcaaggg agcaaaatgg
    11101 tcagataaca tttgggacag acaagataag ttacgtagca tcatatctca aagcgtccaa
    11161 agtgctatcc taaaaggtaa aaatggctta actattgcaa gagatattag acgagagttt
    11221 gatgtgtcag catcttacgc aaagcgacta gcgataacgg agcatgcaag ggttcagatg
    11281 gaagttggta gattatccat ggcggagaat ggctttgcta tgtttgatat attgcctgag
    11341 cctaaagcat gtgatgtttg caaggatata gctaagcatg gtccatatca ccttgacaag
    11401 tggagaatag gggaaaactc tccgccgttt catccgtatt gtcgttgtgc agttgtagga
    11461 ttggataaga aacaagaaga aaaagtgaat gataaatctg aaaaatttga aaacataaca
    11521 ccgaatctca tgccattatt tagtaagttt gcaagtaaaa taacagattt gcaacgtaaa
    11581 atagtgtact ctgctgattt agcagatacc gggtacatta gaacaccgca tgcgtttgat
    11641 ataaacaata ccttgcgaaa taaaggctac aattatctaa acgttgatga taaactaatt
    11701 accgacacat tggatagtgt catctcgata aattcaactc ccaaaaatat aaaagtatac
    11761 cgatttgatg attttgagtt attaggttca atcaacgaac aaaacaataa tatttttgat
    11821 tcaggtaatt ttatggataa attaaaccag ggaggattat catacaccaa cgatggatat
    11881 acttctgcaa gttatgatgt taaaaaaaac gtgatgggat atcgtcctat taagactgaa
    11941 ataaaagtac ctaaaggaag ccacgtttat cttacagata atgaggaaga aagtgaaatt
    12001 atactaccac gagggacaaa gtatgatata attaatgcga agataaatga atatgaagaa
    12061 atagaaatca ctatggaaat aagaaaggag taagcgatgg atttttctga ttttttgaat
    12121 aaaaaacaaa aagagtggga tgaatctcat ccaattcctg actttagtgc aatgagtgat
    12181 gaagaattgc tttatcagcc aatgagtgaa gctttagtgt ctgaaagatt tgctaaagag
    12241 ttatctaaag aggttagaaa gcgtaattta ttatcaaaat aaaaacatat tattatagac
    12301 ggaagactgt ggtcgcccgt ctttttttgt acccaaaaac aggaggaaaa catgaatcgt
    12361 gaaagtaaac caggtatgga aagcgtaaaa atcggagggt tgacatactc tgttgaaaag
    12421 gcatcggacc tgcaaggcaa agatggcaat tggggtctta tacactacaa aacacaacaa
    12481 attaaactag atgactcatt aaccgagcag ctcgaagatc aaacacttat ccacgaaatt
    12541 gttcatggaa ttttagttga ggcaggctat actaatcacg aagaggacca agctaatcgc
    12601 attggtctga ttttgtatca agtattgcag gataatgatt ttagctggtt gtacaaaggg
    12661 gaataaatat gaataaacgc atcaagaaaa aacgtaagtt agaaacagca gttgtgatgc
    12721 ttgttgcaga aaatgctata caagcaaaag ctcttagaaa tcaaaacaaa caaattgcag
    12781 agctgagagc gattatacaa caaaacgtcc aagcaataaa tagagagttt gtaactgcta
    12841 aaaacacgat tttagataac caattagcta ttaagttaat tggtgatgat gttggtcaca
    12901 tcaagcaaaa ttataagcgg aagtggcgaa aataaatttt aaactggtcg aattcgaccc
    12961 ctttagaatc aaactaagtc gtaggaatac ggttttttta ttatgtccaa gcattgacga
    13021 cgataaaagc tatggaatta ataatcgggg acgatttaaa acataggagg tgccaatcat
    13081 ggcagaagaa acacaagtag ttgagatggt tgaagagcaa gtggtacctg aagcaaaaca
    13141 accgcaagac gaaaaaaagt atacagatgc agatgtggac gctatcatcg acaaaaagtt
    13201 tgcgaaatgg aagtcagaac aagaagctga gaaatcggaa gccaagaaaa tggctaagat
    13261 gaacgagaaa gagaaagcag actacgaaaa gcagaagctg ttagacgaat tgcaagaact
    13321 aaaaaacgat aagacacgca atgagttaac agcagtagct cgtcaaatgt ttgcagaatc
    13381 tgaaatcaac gtcaacgatg acgtacttgg tttagttgtg actttggacg cagaacaaac
    13441 aaaagcaaat gtaacaacgc tagcaaacgc atttgctaaa gttatcgctg atgaccgcaa
    13501 ggctcttgta cgccagacca ctccgtcaac aggcggtgga ttgaggaaac aaaccaatta
    13561 cggtgctaac ttggctagta aggcagcaca acaaagcacc aagctttttt aggaggaaat
    13621 tatgaacaaa cgtaaagtaa caacatctaa agagattcta cacaatctcg actatgaagc
    13681 tatttcagta actttggatt caaaaaaaat cggcaaggaa gttgttccag ctgggacagt
    13741 gttagcaggt gtttcaggat cagtatttga agaccgcaaa cagaaagtta aagcggtcga
    13801 taacagtgcc gtagaaagtg aggataacat ctgcgggatt ttgcttacag atgtcgattt
    13861 aacaaatggc gatgcagtcg gttcctgtat ttatcgtggg actatcaatg cagacaagct
    13921 tgctgattca tctattgcgg gaaaatatga agtgttggaa gaagaactac cgcacattgt
    13981 ctttatcaag ggaggtaaat aaacatggca ttgattcacg aaattatcac atcggaaaat
    14041 atcaaaggtt tttacaatgc taaaaacgaa aatgtcggaa acacgctagg ggaaaacgca
    14101 tttcccccaa aacaacaatt aggactcaaa ctttcgttta ttaaaggtgc agcaggcaaa
    14161 cctgtcacac ttaaagcggc agcctttgat acaaaagtgc cactacgtga ccgtatgact
    14221 gttgaattac tcgacgaaga aatgccattc ttcaaagagg ctatggttgt caaggaagca
    14281 gaccgtcaac aactcagttt gttagctcaa actaaaaatc aagagcttat tgacacagcg
    14341 ctagcagcga tttacaacga taaagtaaca cttattgcag gtgcaaaagc acgtctagaa
    14401 gcaatgcgta tggaagtatt gtctaaaggt aaaatccatg ttgcatcaaa cggtgtcatg
    14461 aaagattttg attatgggtt agattcttcg caaacaacta agccagatac aaaatgggaa
    14521 caagtagata ccgctacacc acttaaagac atcgaaaaag ctatcgaagt aatggcagaa
    14581 cgtggctttg tgccagaagc tatcatcatg aactctaaga cacttagcct gattaaaaat
    14641 gcaagtaaca ctttagacgt tgtaaagcct atggcgccag atggggcagc tgttactaaa
    14701 ggtgatttgg aaacttatat tgctgatgaa ttaggtctta aaattttgct taaggatgga
    14761 atgtttgtcg gagacgacgg taaagctaaa aaatacttcc cagatggatt tgcaacttta
    14821 gttcctaacg gcaatcttgg ttacacagta tttggtacta ctccagagca atcagatttg
    14881 cttggtggtg aggcaacaga tgctgaggtc tctattgttg aaacaggtat tgcaattacc
    14941 actactaaga caactgaccc agtgaacgtc caaacaaaag tttctatgat tgctttgcca
    15001 tcgtttgaac gcttggaaga agtacaaatc attaatgcaa cagaatccga agaagaaact
    15061 aaaaaagaaa acagctttga aatgtaggag gtcaatatgc ctagagtaat tagagcattt
    15121 aaagataaag taacaaaagt agtctacgaa gtcggcgata tttactcggg cgaccgagta
    15181 gagtttttga cagagggtgg tgttttagaa ccgtccgtag actttgacaa gctgaaagtg
    15241 agtgagatta aaagcaaact tgacgagtta agcattgagt atgatgctaa acttaaaaaa
    15301 tccgagctat tgaagctttt aaagcaaaca atcggatagt ttggaggtgt ttatggatgc
    15361 agtaaacaca agtagcgtta taagcaatgt aaagcttgat ttaggcatct tagacagtca
    15421 acaggacgat ttacttaaca tgttgctaaa acgcgttaca gaccatttta aagctaaata
    15481 tggcgctgtc gaaatagaca gcgcttttag ttttgtttta gaagattgtt taattgctag
    15541 atttaaccgt agaggtgccg agagggcaaa aagcgagagt gtggaaggtc atacgacaac
    15601 atactacgac tttttggatg agtttgaacc ttacgatgcc atgattatgg caaagcttaa
    15661 tttaatcaaa gacaaatctc gtaaaggggg actgtacttt ttatgagata tgcagataga
    15721 gttacatttg ttaaaacgac ggatgagcaa tacaatcccg atttaggtga gtatacgcac
    15781 acagaggtca taagtatcac aaaaccttgt tttgtgatgg acatgggcat ggaaaagtcc
    15841 gtacagattt ttggagatta tcaaaaggat cgtaaagtta tctacctgaa gcagccttat
    15901 acaaaagcat ttgattattg cgagtatgag ggcaagagat acaaagcgca ggcaaataag
    15961 cttggcgcta ttgtttttta tctggaagga gatgattcta ttggtggctg atatatcttt
    16021 aaaagtagtt ggaacagctg gtttaaaaaa gaaacttgag cttattgtca aaaaagatgc
    16081 cgtcaagaag attgtcagag acaatgggac gcagcttcaa aggaaaatga ttaataaagc
    16141 ggtatttaca aaaggctatt caacaggtgc aactagacgt tctattacca tgcaaatcgg
    16201 cgatggtgga ttgagtgtca aggttaaacc aggaactcat tatgccggct accttgaaag
    16261 aggaactcgc cttatgagca aacaaccgtt tgttttgcca gctctaaaag agcaaaaagt
    16321 aaaatttaga aaagatttgg aggcgcttgt caaatgatta aaactagaga tcagtctatt
    16381 tttgacgaaa tgtttaaacg catccagtct ttaggtttta aggtttacga ttataaaccg
    16441 atgacggaag ttccatatcc atttgtggaa atggaatcta ctgatgcgga atatattcca
    16501 aataaagatg acattaaagg ttctgttgaa cttatgttgt ctgtttgggg ggtgcagaaa
    16561 aaacggaagc aggtgtctga catggcatct gctatttttt cgcaagctat gtccgtagct
    16621 cgtaccgatg gattctgttg gtcgtttaat attaggcagt cgtctataca gatgttagat
    16681 gatacaacaa ctgtgacacc tcttaaacga gcgattgtca cacttagatt taatttgaga
    16741 taggaggtag taatgtcaga acctagcaaa gcaacaacac aggaggagaa agaagtaatg
    16801 tcaaaagcac aagaacaaac aaaacaatta gaagcaaaac aaggaattca ttcaatcttg
    16861 ttatttcgtt tgttgaaaga agcatctagt gaggtagcaa ctaaacttgc ttttcaaaca
    16921 gagcatgaag taggtaaaag ccgtgacgta gatggacaaa aaactaaaga tggtattatc
    16981 cagtccgtgg gggctttgga gtacgacttt aaagcaacat ctattttagc caaaggtgac
    17041 gaactagcag ctaaactaga aaaagccatg gaggatggtg agcttgtaga aatttgggat
    17101 attgattcag aagaaataag caaaaacggc gacagcgaca ataagcttgc aaaagtttgg
    17161 ggtattaata aaaatggaac aaacggagga aacggtaaat gcctagcgac ttattaccaa
    17221 gggtacattt caagcttcag cgctaagaaa aacgcagaag aaaatattgt aattgaaatg
    17281 gagtttgcca tcaatggtgt tggtaaaaaa gggttcgcta cattaacaga tgcccaaaaa
    17341 gcagctgtac agtacgcatt taaagataca acaaaatcca acagcgaaga aactaaaaaa
    17401 gaaaacagct ttgagatgta acggttaggt tggatttagt atccaacctt ttatttttaa
    17461 aggagaaaag aataatgcaa ttggaaatta aaggtaaaac tcataacgta aaatttggta
    17521 cacgatttgt tgccgagatg gacaaaaatc atatagcaga acgtcaggga tttaaatttg
    17581 gagctggtct acaatctagc gttccgtttt taatcgacca cagcgttgtt actcttgcgg
    17641 aagtaattta tacaggtaca atcaccgaac caccacgtcc tagtttgaat gatatttacg
    17701 actatattga cgaagttgaa gatattgaaa agctttttga tgatgtttta gacgaactac
    17761 gtcaatcaaa cgcatcaaag ttgtttatgg ctcaggtgga aaaagacctg gcggaagcca
    17821 aagcgtaaca aatataaaaa agagttattc ttctcaagaa agctttgaga cgattgtgct
    17881 taattgcatt agataccttg gcatgactga catcaatgaa atcgggcgat taactttgta
    17941 tgaatatgat ttattaatga caggcaaagc actagcggct gttgatgaat cacataaagc
    18001 tcacaaacaa gcttggataa accaccaagt tacggcaaca aagcttgttg gtggcaagaa
    18061 aaataaaaaa gaagtccctg tttataaaaa attcaaggac ttttttgatt atgaggaaga
    18121 aatccgaaaa atcactcaag aaattgatga aggttacgac aagaaaggta tggatttact
    18181 tctcaaagct aacctttaag gaaaggaggt taaatgggag aatcttattc tgttgaagcg
    18241 gttttgacag ctgttgataa aacctttggc aaaacattac aatcggcaat ccgttcaatc
    18301 gaaggcttgg aaaagcgttc aaccggtttt tcatcggtgt ctcaaaaagc tagttccatg
    18361 tttaaatcca tgttaggagc gaatttagcc ggacaagcta tctcggcaat gacaaggaca
    18421 gtgtcatcag gccttggctc tatgcttggc gagatgaata gttcagcgaa agcgtggaaa
    18481 acttttgacg ccaatttagc ggacattgga tttggaaaaa aacaaatttt ggcagctaaa
    18541 acggcgatgc aagactatgc aactaaaaca atctactcgg catcagatat ggctagcacg
    18601 tatgcacagt tagcagcggt tggcgtgaaa gataccggaa agctcgtaaa agcttttggc
    18661 ggtttagctg catctgctga aaatccgaag caggccatga agtctatcag tcaacaaatg
    18721 acgcaagcag taggaagacc aacagttgca tggcaagact ttaggataat gctggaacag
    18781 gcgcctgcag ggatggctaa agtcgctaaa tctatgggta aaaatcttga tgaactcgtc
    18841 gccgatatcc aggcgggtag ggttaaaacc agcgattttt tggaagcggt aaaaaaagca
    18901 ggcaatgata agagtttcca aaagatggca actgagttca aaactgttga ccaagccatc
    18961 gacggtatgc gagaaggctt atccaacaaa ttgcaaccag cgtttgaaaa agtgaaccaa
    19021 tttggaatta gagcgatcga agcaatcggt aaacaactcg ataaagttga tttttctaag
    19081 tttgctagta atcttgggaa attccttgaa ggaattaata tcgataaaat tgtatctaat
    19141 atttcatcgg cgatttcatc tgtcacttca aaggttaaag aattttggga cggtttcaaa
    19201 caaactggag caattagtgc tttttcagga gctttgcaga gcgtttggga agctttaaaa
    19261 aatgtcgcta gcgccatgag cggaggaaat tggaagactt ttggagcaac agttggaggg
    19321 actgttaaac acgtctctaa cttcgctaaa gctgtttccg atgttttagg aaagatggac
    19381 cctggcagac taagaagttg gatagctacc ttcgccgcag tagctggagg ctttaaatta
    19441 ttcgaaaaat taacggggca aagcgtcatt ggttcttttt tggataaaat tggcagcaaa
    19501 tttggcctct ttggaaacaa agccaaagaa ggaacagaca aagcctctaa cggcgctaga
    19561 agaagcggtg gcattattag ccaaatcttc agcggcttgg gtaatatcgt taagtctgct
    19621 ggtacagcca tatcaacagc tgcaaaaggt atcggagctg gtattaaaac tgctttgtct
    19681 ggaatccccc ctatcattag ttctctagga accgcaatat caacagttgc gcaaggtata
    19741 ggcactgggc tagcaatcgc atttaaaggc cttggttctg ctattgccat ggtgccgcct
    19801 accacatggc tagctttagg agcggcaatt ttaatggttg gtgctgcttt tgccttggca
    19861 ggaactcaag ctgatggcat tagtcaaatt ttaagaaccg ttggcgatgt tgttgtgcaa
    19921 atcttacaac aggtcactga tagtctagcc actttactac ctattatcgc aaacgctatt
    19981 ggctctatgt tgccaattgt agctggagct atctctcaga ttgtaggcgc agtagcgggc
    20041 ggattatctc agctcgttat agccgtttca acaggggcat ctctcgttat aggggctttc
    20101 acaggacttc ttggtggtat ttctggggtt attaactcca ttagcgctgt tatccaatcg
    20161 ctaactggtg tgattaccgc agtattcaat ggcatagcta ctgttatttc atctgtcggt
    20221 tcggctatca aagatgtatt gacgggtcta ggaaccgctt ttgaaggatt tggaaatggt
    20281 gtaaaatcag ctctagaagg tgttggggca gtaattgaat cgtttggtag tgcagttagg
    20341 aatgtccttg acggtgttgc aaatatcctt gattctatgg gtactgcggc acttaatgca
    20401 ggccgtggcg taaaagagat ggctaaaggc attaaaatgc ttgttgattt atcccttgga
    20461 gatttggttg ctacattagc agctgtggca agcggtctag ggaagatggc tagctcagct
    20521 ggcgaaatga caacattagg ttctgctatg agcaaggtag ccaatggtat gacacgtcta
    20581 gcaacaagtg ctacgatagc aattactgga ttaacagtct ttgccaccac catggcaact
    20641 attaagacag cagttgcaac tctaccgcca gtcctaacga tggcagcgag tgggtttacc
    20701 acatttacta ctcaggcggt ggcagcagtg actggattgg ctgcaattaa tgctccaatc
    20761 actatgttta aagctcaact aatgacaata acaccagctc tagcacaagc tggcgctggc
    20821 tttgccgcgt ttgttgctca atcatcaaca tttagtacag gtttagcatc tgccggtcct
    20881 acaatagcag cgttcaatgc taatttgatg agcttatctg caacaacagg agcgctagtt
    20941 gcatcaatag ctggtttatc agctgtgctt tctgttgtat cagctggctt tagccaaata
    21001 ggggcttctg cgacagcaac tgttggtcaa atacaagctt ttgcttctag tacaacagtt
    21061 gtttcgtcag catttgctag catgcaatct atgattcaat ctgccatggc tgcaatagta
    21121 agcagcatta taacatcatt taatcaagcg gcctctcaaa tgcaatcaat cttatctcga
    21181 atgctatctc aggctaggac atttgggtct caactagagc aacaaatgag acaatcggga
    21241 cagcgttcag gacaaaatct tgctcgggga ctatcttctc aacaaagtgc tgttattaat
    21301 gctatttcta gcatggttaa tgctgcggta tcaagagcca acgcgggagc tggtcctatg
    21361 cgtcaagctg gagcgtacat cggacaaggg cttgcgcaag gaatgtattc agcgctagga
    21421 gctgtaacag ctgcagcaaa cgcccttgta gcacaagccg agagagcagc aagagccaag
    21481 gcgatgattc attcgccgtc aaggttgttt gcaaaacgag ttggtcaata tatcccgcaa
    21541 ggggtagcta tgggtatcga caaaaacgct gatgtcgttg acgactctgt tggcgggtta
    21601 tttgatagca tcaatagctt tgattttaat atcgcagata gactggctag cattggagct
    21661 aaattccaag gtgttatcaa atcagagagt tcacaatcgt tatcgcagca acaagagttc
    21721 gtacatacag ctcaaccagc gtatataaac tttagtttag gtggaaacga atacgaagca
    21781 tttgtaagtg acatcactaa tcaacaagca aaaattgaaa aaatcagact aaagagaagc
    21841 agctggtagt tgtttctctt agttttttga aaggagtaaa atgtacgaat ttaacgatac
    21901 tatcagaggt actccgaaag ttacttttaa tttaaagaca acaattggtg aaaaggtatt
    21961 agaagaagaa ctcaataatg atttaggtac ctttaggaca ttgactgttt ctggtcgtga
    22021 tgtggtggac ttagagcatc aaacaacaag tgttttaggg cgaaatggtg agtatttcca
    22081 caatgccaca gtcgaagtca gaaaactaga aataaaagct aaaatcactg gaaaagataa
    22141 ccagtcaatg cgtttgcaat atgaaaaatt aaacagatta attgttagtc acaatcaagt
    22201 ttttttatca ttttcagacg aacctgacag aaattatcta ggcatcttta aatctaaaga
    22261 tgtcccagaa gaagtttcta acgagcagat tataggacta acattcatct gctacaatcc
    22321 gtttaaaatg tctgatgtaa aaactaaaaa aggaacatct atccaaaatg gtgggttatt
    22381 tcaaacaaag cctatcatca ctctcaacct atcatcacca acaaaagaaa ttaaattgtt
    22441 gcatgtcgaa agccagaagt atatcagatt gactggaact tataccactg acgaaatcaa
    22501 gattgatatg gccacgggta aaattaccca gaacggtcgc aacatccttg gagatttaga
    22561 tatgattaat agtaggtatt ttgagctatt acctggtaat aatacattac agtgtgccaa
    22621 tgccgccata accgcagagt ttagagaggt ttatctatga tatacctgtt tgataaactt
    22681 gagcgattgg tcgctactgt tggtactgat gacttgctct catggcactt taaggtcaaa
    22741 aacaatgatt gggaccaggc tagttttgaa gtgccagttg attatgacgt agagccattt
    22801 gtctactttg gtttttttaa ctacgaccct aatcaaaaag aagatgtctt taagctcttt
    22861 aaagtcattg attacaacct agaagatagc aagttttaca aaggcttaga caaagctgag
    22921 agtgatcttg ataccattgc cattatcaaa gataagcgct ttagacaatc gtccgcagat
    22981 gcttgtattg atggtgcttt agagggtacg ggttatcaag ttggcaaagt tgagggaata
    23041 acaaatgtta gaacacttag ttattactac atcagtccac gagcggctct gattaagatt
    23101 gtggaagctt ttaactgcga gttcaatgtc aggtatacct ttattaataa caaaatcact
    23161 agtcgttata tcgacctcaa aaagcgcttt ggaaagccaa caggcaagca atttgaacat
    23221 ggcaacaacc tgttaaaagt cgtctacgag gaatcaacag atgacattgt gacttgtctg
    23281 attggcagag gtaagggtga ggaaatccaa cacgaagaag ctgagcctaa agatgtcgag
    23341 ggacacttgc cacaggaaga aaggcggcaa ggctacggtc gaagaatcga atttactgat
    23401 gttgtctggt cggtcgaaaa aggcgacccg atagacaaac cagctggtca aaactttgta
    23461 gcactagata gtgcaaggga agaatacggc ttgtctcaaa atggtgagct aaaacaccgc
    23521 tggggtgtct ttgtcaatga ggaaatcgag gataagacag aactcttaaa agcaacttgg
    23581 gaagaattgc agcgtttgtc aatccctatc agaatttaca aggcagaaat cttagacatt
    23641 ggtccagaga cgtggaaagg cgactcagta gccattatct atgatgaggt aaaaatagct
    23701 tttgaaactc gggttgatga gattgatatt gacaagctta attttaacag gtcagtcgta
    23761 acacttggtg attacagcgt tgtccaaaat cgtgagtcac ggtctcgtaa agaggctgtc
    23821 caaaacatga tagatgaatc tttagagact atcacagact taggaatgac atttcaggaa
    23881 tttttgcaag gcatcgaaaa acgcatcgag actggcaaaa aggagatgga agacaattgg
    23941 cgaaaagtta accttgaatt cgataacttt aaaaagaagg ttgagcaaga aggcttgcaa
    24001 ttcaacacct tgaaagaaca aatcaaagaa gttgatgaac gcaccgacaa agagctagag
    24061 gaattccgag ccaccctcaa aaacctagcg ttaccagagg aagcgattaa aaaaatcaca
    24121 gaggctatca aagttgatga catcccgtct cttaagcaaa actttgatga cttaaaaaac
    24181 aaggtcagcg agacaagcga gacggcacgt ctaaatgccg agattatcgg tacagacggt
    24241 aagacccgct ataacaaaaa tttattggtt ggcgacccta accgtgtaaa aacctatgac
    24301 caagactata tcgaggtaga agctaatgat ggcggcttta agcgtggcga gacctacacg
    24361 attagcttta gtcaaacgtg tgagctactc aaaaaagtag ctatcacatt gacgcaaatt
    24421 aacaacaagg gagttaagtt agtactgaca ccaaccaaag caaaaatgga tgcacagacg
    24481 tttgaggtca ctaaagataa acagtctata gaggtttatc ctttgagcta tacaggcgtt
    24541 ttaacaggtg attggtataa atctaagtct gtggagataa acgcctcaga ggcgcaggaa
    24601 ttagctcttg agatggctta taaagagatt gcagatgcca aaggtgcaac tattatcggc
    24661 aaacagtccg ataaaccaaa aattatttta gatgggagga gggacagatg acatcagtcg
    24721 aaacaatacc aataaaaatt gtctttgacc gaaaagacgc ttcagaatgg cagtcaacta
    24781 accctgtcat tgacgaaggt gaactagtcg tcgagctaga cacccataag ctaaaggtcg
    24841 gagatggtaa aacaagctac aatgacttgc cttactatga aggtccgcaa ggagaatcca
    24901 taacaaaagt acaactatcc gaaaatggtg acttgtctgt gtggattggc gacaaagaga
    24961 ctaagcttgg caacatcaaa ggtcaaaaag gggacaaagg gacaagtata accgacatca
    25021 ccaaagatgg tgagacactc actatcaaac tatcagacga cactcaaaaa atctttaata
    25081 tccccaatgg ccaaaaaggg gatagaggta agggcgtaga gagcgctagg attgacgaat
    25141 ccggtcattt aaaattaaaa attgaagagg aatcagagtt agatcttgga aacgttaaag
    25201 gtgagtcagg acctaaaggt gatagtatta ctatcacaaa tcacaagcgt gtttcagatg
    25261 gaacgcaggt atctttcagc gacgggacac aaattgttgt cccaaaaggg gatactggtg
    25321 atgtcaacgg catcaatctg gaagattatg tcaaaaaatc tgaacttaaa aacgtcggtt
    25381 ctgcagatgt taaagctatt aatgactttt tagggctatc tcaaaaggtg tttacaagca
    25441 gctatagtta tacagatagc ttactaaaaa gttatgcaaa acccagctat tcggcgagtt
    25501 ggtatgtcaa cgaatctaca gtttctacca aaaatggtga caaagtattg attacaatac
    25561 ataacactac cacccaagca gacaattatt tggaggtagc ggtgacttat gttggtgcta
    25621 actacgtgac tgctacctca acaggtcgct tactgactac tcctggtgaa gtcaaagtag
    25681 tgacaaaaaa gcaagcggaa aaagattatg ctgctaaaaa acataagcat gagattagtg
    25741 acatagctgg tcttaatgag cgcttgtctg gctatctcag acaagctgat atacagtcac
    25801 agcttaataa tatcggtaag ctaaaagaca cgcaaaccgg acaatatctt gaggttaaag
    25861 tggttgataa aggacaggtg cctagcaata ccagtggcat gatcgtgttt gagaggtcat
    25921 aatttgttaa aagatttaaa taatatcatt attaataaca aaacgattga tcgtattatt
    25981 tgtaataata cgattgtcta tcagcgttcg tgggatctgc tttttgacgg tttgctgaca
    26041 agcgctggga aaaaattgga tatttatcct ttttataaaa tcgtaatcaa taatgcattt
    26101 tttgttaaaa cccccacaac aaaaaacaat acgatttttt tagcagggca taaaatacta
    26161 gcattacaag atagtcattt ttgttttttt agagcattag aaaaaagttt ttttgtcaaa
    26221 atttacggta aaaattagga ggtaacattt gagtagagac ccaacacttt taatagacga
    26281 gtcaaattta acaatcggct cagatggacg tgcttattat acatttacgg ctgatggtga
    26341 cacaaaaagc gttaaaatag ccaataacaa atgtatcggt acaactcgct ttaaccagct
    26401 catgattgag cgagggggta aaccaactaa ctacgtggcg cccgtggttg tcgaggggac
    26461 aggtaatccg actggactat ttaaagacct caaagagatt agcctcgagt taacagatac
    26521 taaaaactcc aaactttggt caaaaatcaa gcttaataat caagggatga ttgaagagta
    26581 ttacaacggt acaataaaat ctgagattat caaaaccgca gagggcacgc agcaacgtat
    26641 tagtagcgag accgataaaa aacttgcgct tatcaacgag acagtctcag gcattagacg
    26701 tgagtaccaa gatgcagata gacagctatc gtcaagctac caagctggta ttgaggggct
    26761 aaaagccaca atggccaatg ataaaatcgg tttacaagct gagatacaaa caaccgctca
    26821 aggcttgtat caaaggtatg ataacgagat acgcaagctg tccgctaaaa taaccactac
    26881 ctcgtcaggc actacagagg cctatgaaag caagctcgat ggcttacgag ctgagtttac
    26941 tcatagtaat caaggtatgc gcgtagagct ggagtcaaaa atcagtgggt tgcaatcaac
    27001 gcaacaagca actgccaggc aaatctcaca agagataagt aaccgtgaag gtgctgtcag
    27061 tcgtgtacaa cagggcctag acagttatca gcggcgatta caaaatgctg aagggagtta
    27121 cagcagctta caacaaactg ttagtggttt acagtctgat gttaatagtc caaacagtaa
    27181 gcttaattct cggattagcc aattagctag tcaaattgac caacgagtca caagagccga
    27241 tgtgacaagc atcattaacc agtctgggga cagtatcaag ttagccatac agagagctgg
    27301 tgggattgat gctaagatgt ccgcaaaaga gattgtctca gccataaacc tcaatggcta
    27361 cggtgtcaga atttctggtg agcgtattgc tttagacggc aataccacgg ttaacggggc
    27421 ttttggcgca aaaattggtg agtttatcaa gctaaaagcc gaccagatta tcggtgggac
    27481 aatcgatgca aacaaaatca atgtgattaa tctcaacgct agcagtattg ttggtttaga
    27541 cgctaatttt attaaagcaa ggattgagta tgcgatcaca agtttgcttg agggtaaagt
    27601 tattaaggct cgtaatggtg cgatgacaat agacttgcaa agcggtcaaa tcaaccatta
    27661 cacaaatgag tcagccatga gacgtatcga tagcagtaca gctagtcagt ttataaagat
    27721 gacaaagtca ggctttatat ctgagatagg taacatgcaa gctgcaatga cggttatagg
    27781 ctctaatagc gatgggtcag aaaaccatga aaataaaacc ttcggaggca taagaatttg
    27841 gaacgggaag tcatcttatc aatcgacaag ttttgttgaa ttagtcggta accgtgtagc
    27901 aatctatgga aataaaaatc gtagtccatg gctctttgac tcaacaacgt caggatatgc
    27961 ttacctcatt ccacagaatg aaagtggtat aaagcatgcc atagggagag ctgatcgtaa
    28021 gatagaccaa atccacgttg gagatatcta tgtgcaaggc gaacgtgtcg ctatgatgtt
    28081 aaaagattta gcgaatagga ttggctatat aggtacaggt ggctgggcta accgcatagg
    28141 ataaattagg agaaacaatg caagaaaaat tattaggaaa aattattaac gatttaacac
    28201 ttaaagtcgc taatttaacg ctggaaaatg ctcaattaaa agcacagcat gaaatcgaat
    28261 tggaagaatt aaacgcacaa ttggatgaag caacagcacc gaaggaagaa ggtaaataaa
    28321 catgagaaat tggaaagtaa caggaaacta cccacagtgg gacggtacag gagcagtcat
    28381 tgatacgcag gtaattatta cagatgacag gggtgcagtc atcgcagaga agatcaaaaa
    28441 agatttgaga acagctaatg atgcagaaat tatcgaggct gctttagagg agtttaaaaa
    28501 gactgcttac gtcgaaatcg ctatgggcga agccgtgcaa aaagtagacg accttgaaaa
    28561 aatctcacag gaaactgcta agactgccaa aactgctcaa acagctgctg gactagctaa
    28621 ggtgtccgca gagcgcacgc aaaagatgat taacttacaa acgatccaca tgttaacgag
    28681 cggcggcaag attgatcctg acatttataa aggcatgctt gagctaatcg agccagccaa
    28741 aaaaggtgag tatcaagcgt atgatgtctt tacggtggtc gacagtacta aagaggaaga
    28801 cggtgaagca ggcgaaggga acctagtctt cgtacacgtt aacgagccgt ttacttatga
    28861 ggcgcaaact cttaaagatt tggaatcaga ggataaagtc acagtcatta aatatgcgga
    28921 tttggttaaa caagattaga ggtggttaga tgatagattt tgtacaaatt ggtgcttttt
    28981 gtggcgctgc tttgtctatc ttgggtgttt ggggatttat cgtcaatccc tttaaaaaag
    29041 ccatggaagc taatgagttt gccatggctc aactcaagga ctcaattaag gagttagctt
    29101 atgagcttaa aaatcttgat cgtgaacgtg agattaccaa aaaaattatc gacagacacg
    29161 aggagcgttt aggtcgcgta gaagacgaag ttattatcaa caaggagcgt attattacgc
    29221 tatttaaaaa aggagaagaa aaatgaataa ctggtttaaa aaagtagcaa ttaaaacaat
    29281 taaaacaatg gcacaaacgg ccgttggtct tatcgggtca agcatgttga ttacggatat
    29341 taactggcca acaatgttgt cagcagtgct gttgtcagga ctaacatgta tcctgatgaa
    29401 tgtgtcacaa atcaaggaag aggaataggt catgcgagca atcacacgat tagcattagt
    29461 tatagcaatc gcaatactgt atgtgccatt atctgtggtt gctttgattt tttatccatt
    29521 tttagataag gagggataaa tggctacata tcaggaatat aaaagtcgtt caaatggcaa
    29581 tgcttacgat attgatggat cgcttggtgc gcaatgttgg gatggctacg cagactactg
    29641 taagtatcta ggactgccat acgcaaactg tacaaataca ggatatgcaa gggatatatg
    29701 ggaacaacgt cacaaaaatg gtattttaaa ctactttgac gaagtagaga ctatgcaagc
    29761 tggagatgtc gcaattttta tggtagttgc aggtgttaca ccgtatagcc atgtggctat
    29821 ttttgatagt gatgcaggta gtggatacgg atggttttta ggtcaaaacc agggtggagc
    29881 aaacggagca tataatttag taaaaatacc atattcaacg acttatccta ctgcatttag
    29941 accaaaagtt tttaaaaatg cagttactgt tataggtaat ataggactaa ataaaggcga
    30001 ttattttatt gatgtatcag cttatcaaca agcagactta accgctactt gtcagcaagc
    30061 tggcactact aaaacgatta tcaaagtatc cgagtcactc gcttggctgt ctgataggca
    30121 tcagcaacaa gctaatacta gtgaccctat tggttattat cactttggac gatttggagg
    30181 agatagcaac ttagcgcaac gagaagcaga tttatttctg tccaatttac caaccaaaaa
    30241 agtctcttac ttagtcattg attatgaaga ctctgcaagt gccgacaaag aagctaacac
    30301 taatgcagtt attgcgttta tggataaaat tgcaaacgct ggatataagc ctgtttatta
    30361 cagctataaa ccatttacgc ttaataatat tgattatcag caaattatcg ctaagtaccc
    30421 aaacagcatt tggatagctg gttatccaga ctacgaagta cgaacagagc cactttggga
    30481 gttcttccct tcaatggatg gtgtgcgctg gtggcagttc acaagtgtag gagtagcagg
    30541 tggtttagat aaaaatattg tattattagc agatgatagt agcaaagtgg atatacctaa
    30601 gattgacaaa ccacaaagcc agcttacttt taatcaaaag ctagatacta atactaaatt
    30661 agacaactcg aatgtacctt actacgaagc aacccttaga acagactatt atgtagagtc
    30721 taagccaaac gcaagtagcg ctgataaaga atttatcaag gcaggaactc gtgtaagagt
    30781 ctatgaaaaa gtgaatggat ggtcacgtat taacgcttct cagtctgacc agtgggtcga
    30841 agataagtat ttatctaatg caacacaagt ataaaatagg aggtaaagct cctttagata
    30901 agacaaaacc gctcagataa tttctgggcg gttttttgtg tatgatgaat tatttttcaa
    30961 gataaatatc gaaatgacta aagttcttca tattgataat tctattatct ttatattttg
    31021 caaaaatatc tgatctagtc ccttcatttg gtgagtcaaa taagtctatt tgctcatgtt
    31081 tcccatcttt tgtgccaatt tcgattctgc cgcttacata aggagaagta gcgtcataaa
    31141 ttttataatt atccataagg tattttctga ttttaaagtc aatttcctgg aaagttacga
    31201 tatctttttc tagaataatt ttgttattta agttctgttg agattctccc gaaataaata
    31261 gatttcccaa taatttatga tttactttat tattttgagc aggcgtaatt cctccataga
    31321 tgtactcacc ggtgtgagaa ttaagaatat aaaataatcc aaaaacatct acatgatcat
    31381 ctcgtttaaa tttttgagag gcctcataag acatttcgga actaatataa tagtctttcc
    31441 ctctatattt ttgagtatca atgtttaatg tgtgtgtcgt tgaaaaattt accctgcaat
    31501 ctttataatc ataaggagtt atagtgtatg cataaagtaa atcactttta acattcgaaa
    31561 tgtctttctt agagtcactt ttgatgatag gtgaaatagt agaaatcagt atgactgtaa
    31621 ttatgaaaac tattttgatg atgttaatct ttttcatttt ttctccttaa ttaaatgtat
    31681 tactcgtacg agaatacatg aaatagcagt aagtagttaa aaatcagatt tctgtttatt
    31741 tagaaatttt ataaatgtat ttatatttta gtttttagga gtggcagttc catttaaata
    31801 gtcaaggttg atatttggag cagagttttc taagattact gtagtaacac cattttcatc
    31861 aatactttct ttattactat ttagccttat agtaagcaac tcaccagatg aatcaattcc
    31921 aacatactgt aattcaattt gtcgaggcac tacttcattg ccactatata taggagtgac
    31981 tttataatct agccaaaagt cagggtgaag tgcaagccaa gaatctaaac ggttttcata
    32041 gtataacatc ccttcaggat tgctgtcatt tgctcctgaa taagcacctg tgtttagcca
    32101 ggctgtcatt gtcactaaat ttcttggttc atcgtttaat ccacaaaatt gatatccgac
    32161 tagatgccca cgattcatta cccatgaaga ttttgagcca tctccgtatg gaaattgata
    32221 gttatgccat cccacagggt cataatttat tttagtgcgt acatctttag tttcgtgtct
    32281 atcttgcagt tggatatgtg aaaaggtagc gcggttaagg ttatccaact caccaagttg
    32341 taactgataa ttagcagtaa aaggtaagag cttactagaa gcagtatttt tataatgtgt
    32401 gtttgcatgg gatacattcg gataagttcg taccttagct gcgtcaacag ttattgtcgt
    32461 tatagaaaga gaaagtaaca gtaaaacagc ggtaagcaaa cttgcctttt gtttagataa
    32521 tttcatgata acacctaaac ctttccattt ttagctttta gagtggatca aatattgcac
    32581 ttttctagtt aattatatca ctttaaatta aaaaaactta aaattaataa aattaattta
    32641 tcaataatat tacgctaaaa tttctttttg tttaataaaa tagaagttat taaattttaa
    32701 taagttttaa atttaagtgt actatattct cgtaaaatac gaataataag ataaggaggt
    32761 gctttatgct aacatacgac gaatttaagc aagcaattga caacggatat atcacaggag
    32821 acacagtaat aatcgtgcgc aaaaacggac agatttttga ttatgtgttg cctggtgaga
    32881 aagtcagacc atgggaggtt gtgaccgagg aggtagtgga agaagtgatg gtggaattag
    32941 actatatcaa atgaagaaaa ctttgagtag acagaaaaat aattgaagag ttgtcaattt
    33001 agttgtcaat aatatagaaa gttatggtaa ctcatgaaca agacaaaaag aaaaaacctt
    33061 gatgtaacaa ggttttagta agttatgatt acttacggta agcattgatg gagctggtgg
    33121 gagtcgaacc cacgtccaaa cacctgccaa catatttgtc tacgtccata ggttatgtct
    33181 tcatttaaca aactaaagac acataactca agccattagt ttgcgagtct atcaatctct
    33241 tgtcacgaaa ctagacaatt tcgtaaccgt agcttgctat tttgagactt ctaacaggac
    33301 acaagcaatc ttgtagaagt cacgctagct ggtttttagg cagctaaagc gtaagagttt
    33361 gtattttttg cagttatatt taactggcaa tttacatctg ccgatgggac gcaaaacatg
    33421 cctcataatg cctgtcgaat ccgtaacaac cccaaataga taattaacca atttcttaca
    33481 ctaactattc tatcaaaact ttttttaaat agcaaaattt tacagcttat ggtatgatta
    33541 gtctatgaca aaacgtaatt caaaagccta tatattatgg caaaaaatta tcaaaatttt
    33601 aggaattatt gcactaattg gtactttttt cctcgctttt tggctgtata gactaggtat
    33661 attaaacgat agtaatgctc ttaaagactt agtgcagcgc tatagattat gggggccgtt
    33721 tgtttttatt gtcgtgcaga tcatacagat tgtttttcct gtgattcctg gaggccttac
    33781 gacagttgca ggttttttaa tctttggccc tgtaacaggt tttatttata attatgtagg
    33841 catcattatt ggcagtatag tcctcttctt gcttgttaag acttatggtc gtaaatttat
    33901 cttactcttt gtaaatgaca aaacatttta caagtatgaa aggcgtttgg agacaccagg
    33961 atatgaaaaa ttatttattt tttgtatggc atcgcctgtt tctccagctg atattatggt
    34021 tatgattacg ggattgacgg acatgtcttt aaagcgcttt gtaactattc ttttaataac
    34081 taaaccaatt tctattatcg gctacagtta tttatttatt tttggaaaag atgttatatc
    34141 ttggttctta cagcattaag gtttggtgtt aatttgataa accgcttcta tttgatggaa
    34201 gacggttttt ttatttacta tgaacactga aaaagacttt cttttaaact atagtgtgtc
    34261 atagtttaaa agaaagtctt aacgcttact gtaggttgag tttatttttg ataataaaat
    34321 tagtggcgag gtaaaacatt atcatttcaa taaattgttc caaaataaga agataaattg
    34381 ttttttgctc agtaaatggg aaactatcgg cacttgtatt aatattaaag atgtgactgt
    34441 gaacatatgt tgcagcaacg ctaatcagaa taactaatat aaaatatgca ataaaggcca
    34501 tcaagcctcg ccgattagaa aaaagttgac ctatagaaat ggaaagataa attaataaga
    34561 tactcgtaaa tgttgataat aggacatagg ctagtacagt tagcatattg atgaaataat
    34621 ccatcttaaa actattaaag aatccttcta atagttcgtt gatgttaaac attggtacaa
    34681 ttacaatagc gatagcaaat gcaaggatga tagtattgaa aacactgcaa ataaaagaag
    34741 ctagtagttt tgataaaata atttgatgcg aattaacggg taaagtcaac gttaagtatc
    34801 cttcccgtcc aaaaacactt ttgctaaaac gtttgataat gataagtaat gttgataaga
    34861 gggaaccagc gatcagggag ccaaaagtta aaccta
//
LOCUS       DAUVUY010000008        31538 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.008, whole genome shotgun sequence.
ACCESSION   DAUVUY010000008 DAUVUY010000000
VERSION     DAUVUY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31538
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.008"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..503
                     /locus_tag="VOX58_000398"
     CDS             <1..503
                     /locus_tag="VOX58_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HES5877664.1"
                     /translation="ALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRLILETFAN
                     IAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKELARLASQ
                     VYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVDRLVTDKN
                     CDSYQP"
     gene            583..1278
                     /locus_tag="VOX58_000399"
     CDS             583..1278
                     /locus_tag="VOX58_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HES5877665.1"
                     /translation="MDFFAIDPIGLPHTSLIFYLSSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLAFAFSKEK"
     gene            1526..3070
                     /locus_tag="VOX58_000400"
     CDS             1526..3070
                     /locus_tag="VOX58_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HES5877666.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNVFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            3377..4996
                     /locus_tag="VOX58_000401"
     CDS             3377..4996
                     /locus_tag="VOX58_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HES5877667.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            5123..7123
                     /locus_tag="VOX58_000402"
     CDS             5123..7123
                     /locus_tag="VOX58_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HES5877668.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKISPWL
                     IIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLISEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYL
                     IKKGTRPILAHLAMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVTNARQLSNFERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(7147..7425)
                     /locus_tag="VOX58_000403"
     CDS             complement(7147..7425)
                     /locus_tag="VOX58_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HES5877669.1"
                     /translation="MTTKKAYMYVLECADKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(7415..8269)
                     /locus_tag="VOX58_000404"
     CDS             complement(7415..8269)
                     /locus_tag="VOX58_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HES5877670.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            8309..9049
                     /locus_tag="VOX58_000405"
     CDS             8309..9049
                     /locus_tag="VOX58_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HES5877671.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKLAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTILFSYIASFVWNPDKHRH"
     gene            9249..9911
                     /locus_tag="VOX58_000406"
     CDS             9249..9911
                     /locus_tag="VOX58_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HES5877672.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            9892..12134
                     /locus_tag="VOX58_000407"
                     /pseudo
     CDS             9892..12134
                     /locus_tag="VOX58_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
     gene            12205..13245
                     /locus_tag="VOX58_000408"
     CDS             12205..13245
                     /locus_tag="VOX58_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HES5877673.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSHQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKVDVARDLIHDLRLSGEDDIKLIAIMLGQFRLYLQLAILAKEGKSEQQL
                     VTSLSEILDRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            13342..13947
                     /locus_tag="VOX58_000409"
     CDS             13342..13947
                     /locus_tag="VOX58_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HES5877674.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            14114..14340
                     /locus_tag="VOX58_000410"
                     /pseudo
     CDS             14114..14340
                     /locus_tag="VOX58_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14325..14573
                     /locus_tag="VOX58_000411"
     CDS             14325..14573
                     /locus_tag="VOX58_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877675.1"
                     /translation="MDADLAQMDALGLYYDDNHLSLVNTVKVYLKEFGIGESQIAFSY
                     KDLNSGRTAAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            14570..15160
                     /locus_tag="VOX58_000412"
     CDS             14570..15160
                     /locus_tag="VOX58_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HES5877676.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVYSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQGLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLITLYSSGL
                     GGATQASEEVNGLGYVQLVQLTYVINEWHRVNHNMI"
     gene            complement(15274..16494)
                     /locus_tag="VOX58_000413"
     CDS             complement(15274..16494)
                     /locus_tag="VOX58_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HES5877677.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQNLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSTLIQAYYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(16501..17529)
                     /gene="queA"
                     /locus_tag="VOX58_000414"
     CDS             complement(16501..17529)
                     /gene="queA"
                     /locus_tag="VOX58_000414"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HES5877678.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            17731..18435
                     /gene="nagB"
                     /locus_tag="VOX58_000415"
     CDS             17731..18435
                     /gene="nagB"
                     /locus_tag="VOX58_000415"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HES5877679.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIKANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            18554..19270
                     /locus_tag="VOX58_000416"
     CDS             18554..19270
                     /locus_tag="VOX58_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HES5877680.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPIHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            19565..20527
                     /locus_tag="VOX58_000417"
     CDS             19565..20527
                     /locus_tag="VOX58_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HES5877681.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFCCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            20540..22345
                     /gene="pepF"
                     /locus_tag="VOX58_000418"
     CDS             20540..22345
                     /gene="pepF"
                     /locus_tag="VOX58_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HES5877682.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFS
                     GHLLDSSANLLTITDTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSIRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVDKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            22721..23917
                     /locus_tag="VOX58_000419"
     CDS             22721..23917
                     /locus_tag="VOX58_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HES5877683.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     ALRLKK"
     gene            23983..24690
                     /locus_tag="VOX58_000420"
     CDS             23983..24690
                     /locus_tag="VOX58_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HES5877684.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQITGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLKVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADILLPD"
     gene            24753..25808
                     /gene="prsA"
                     /locus_tag="VOX58_000421"
     CDS             24753..25808
                     /gene="prsA"
                     /locus_tag="VOX58_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HES5877685.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            26195..28813
                     /gene="alaS"
                     /locus_tag="VOX58_000422"
     CDS             26195..28813
                     /gene="alaS"
                     /locus_tag="VOX58_000422"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HES5877686.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            29174..29389
                     /locus_tag="VOX58_000423"
     CDS             29174..29389
                     /locus_tag="VOX58_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5877687.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            29386..30147
                     /locus_tag="VOX58_000424"
     CDS             29386..30147
                     /locus_tag="VOX58_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HES5877688.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            30166..30861
                     /locus_tag="VOX58_000425"
     CDS             30166..30861
                     /locus_tag="VOX58_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HES5877689.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIAGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(30955..31482)
                     /locus_tag="VOX58_000426"
                     /pseudo
     CDS             complement(30955..31482)
                     /locus_tag="VOX58_000426"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         9582 a         5571 c         6535 g         9850 t
ORIGIN      
        1 ctgctttaca gactgtgacg ttgacaggta atcgtacgga gtggaaaaaa gcagccaatg
       61 gtgctgatat tttatcagat gtctacaatg ccaacccaac agccatgcgt ctgattttag
      121 aaacatttgc taacatagca aagaaccccg gaggaaaaaa gattgcagtg ctagcagata
      181 tgaaagaact tgggaaggat tcagtgatac ttcattctca gttaattgac agcttaacat
      241 caggtaatat tgatcagctt gttttttatg gggatcatat taaagaatta gcacggttgg
      301 caagtcaggt ttatcctgct gagcaagtac attatttttt aaaaactgaa caagaagacc
      361 agtttgaagc catggcacaa tatgttcaaa acattcttaa tccctttgat caaattcttt
      421 taaaaggcag ccactcgatg tcgctagaaa agttagtgga tcgattagtg actgacaaga
      481 attgtgatag ctatcagcct tgatttttat aaaactttca gataaagtct tgttctctca
      541 tgaagattaa ggtataatga aaaaatatag aaagggacga ttatggattt ttttgcaatt
      601 gaccccatcg gactgcctca tacctcactt atcttttact tgtcgtcact cttgatagcg
      661 cttttgcttg tttttttgac ttttcaagca tataggttaa aatcttatcg atattttttc
      721 ttgtttttac agttatctca ggtgattggc ttgtacacat ggtacgtgtt aagggggttt
      781 cctttagatg aagctttgcc gttgtatcat tgtcgcatcg ctatgttagc tatctttttt
      841 ctacctgatc gtaacaagtt taagcaatta tttatggttt tggggatagg tggaacattc
      901 cttgcacttt tatcgccaga tctttatccg tttagattat ggcatgtagc aaatgtctcg
      961 ttttatcttg gtcattatgc tttgttagtg aatggtttga tttacctctt gcgtttttac
     1021 gatgccagtc agttaagact gctatcagta gtacgctatt tagcaactgt taactttctt
     1081 cttctgttgg tcagtttagc cacgaaagga aattatggtt ttgtgatgga tattccagtg
     1141 attcatacgc gtcatttgct acttaatttt gtgatcgtta caagtggctt gacttttatg
     1201 gttaaaataa ccgaatattt ttaccttaaa tttggtgaag ctcaacagct ggcatttgct
     1261 ttctctaaag aaaagtaggt cgttgaaaaa gcacttaaaa tcagctataa taagaagagt
     1321 ctgggatgat ttccaggctc gattttttta aaaaagatac tcattgatag cccaagataa
     1381 gttataatac cagttttgaa gaactttaga ggctaatcgt atatggagaa acccaatgta
     1441 gcccaactct tttgataaac aagtaatacc taacgaaaat aactcagaga aaagctctgc
     1501 cattttacta tacaaggaaa aaagaatgtc acttacagaa gaaattaaaa aacgccgtac
     1561 ttttgccatc atctctcacc cggatgctgg taagacgact attactgagc aactattata
     1621 ttttggtggt gaaattagag aagcagggac agtaaaaggg aaaaaatcag gtacgtttgc
     1681 aaagtccgac tggatggata ttgaaaagca acgtggtatc tctgttactt catctgttat
     1741 gcaatttgat tacgcgggta aacgtgttaa tattttggat acacctggac atgaggattt
     1801 ctcggaagat acataccgta ccttaatggc tgtggatgcg gctgtcatgg ttgtagattc
     1861 ggctaaaggt attgaggcgc aaacgaaaaa actttttgaa gttgtcaaac atcgtaatat
     1921 tcctgtcttc acctttatta acaagttaga ccgtgatggg cgtgagcctt tagaactctt
     1981 ggaagaatta gaagaagttt tagggattgc ttcttatccg atgaactggc cgattggtat
     2041 gggacgcgcc tttgagggct tatatgattt acataataaa cgtttagaac tctataaggg
     2101 agacgagcgt tttgcaagta ttgaagacgg tgatcagctg tttgccaata atccctttta
     2161 tgaacaagtc aaagaagata ttgaacttct tcaagaagca ggtaatgtct tttcagaaca
     2221 agctattctt gatggtgacc taactccagt cttttttggc tctgccctta cgaactttgg
     2281 agttcagact tttcttgata ctttcttgga gtttgcccca gaacctcatg gtcacaagac
     2341 tacagaaggt aatgttgttg accccttagc taaagatttt tcaggttttg tctttaaaat
     2401 tcaagctaac atggacccta aacaccgtga ccgtattgct tttgtgcgta ttgtttctgg
     2461 tgaatttgag cgcggtatgg gagttaattt gacacgcact ggcaaaggcg ctaagctctc
     2521 caatgttact caatttatgg cagaatcacg ggaaaatgtg acgaatgctg ttgctgggga
     2581 tattatcggg gtttatgata caggaactta tcaagttggg gataccttaa cagttgggaa
     2641 aaataaattt gaatttgaac ccttaccaac ttttacccca gaaatcttca tgaaggtttc
     2701 tccgaaaaat gttatgaagc agaaatcctt ccacaaaggt attgaacaat tagtacaaga
     2761 aggagcaatt cagctttata agaattatca gacaggcgaa tacatgctgg gtgctgtggg
     2821 tcaactgcag tttgaagtct ttaaacatcg tatggaaggt gaatacaacg ctgaagttgt
     2881 gatgacacca atgggtaaaa agactgttcg ctggatttca gaagatgatt tggatcaacg
     2941 catgtcctca agccgaaaca ttttagctaa ggatcgtttt gaccaaccag ttttcctatt
     3001 tgaaaatgat tttgcccttc gctggtttgc agacaaatac ccagatgtca ctttagaaga
     3061 gaaaatgtaa tcgaaacatg attttcaaca gtctaacgtt ttggagatca atttactgag
     3121 caacttttta aaagaaatga gtggctagtt atctcttttg cagatttaag attctatcaa
     3181 aaaacgatat cttcacaact gtaagcatat ttatgattta gcggataatc gtgaaagcgt
     3241 gttattgctt atattagcct tatttcctat ctttttgatt ctaattgtgt tacactagtt
     3301 aggttgttaa tcaaacgacg aaaattaaaa ggtttataag gcagcttcgc actgcctttt
     3361 tagaaaagag aaacaattga aatttacaga atttaactta tcacaagaca tccaatcagc
     3421 tgttgttacc gctggttttg agaaagcatc tcctatccag gaaatgacga ttcctctcgc
     3481 actagaaggg aaggatgtta ttggtcaagc acaaacagga actggtaaaa cagcagcgtt
     3541 tggtctacca accttgaata agattcgtac taacgaaaat atcatccaag cattggtcat
     3601 tgcaccaacg cgtgaattgg ctgtacaaag tcaggaagaa ttgttccgtt ttggacgtga
     3661 aaaaggggtt aaggttcgct ctgtttacgg tggatcaagt attgaaaaac aaatcaaagc
     3721 ccttaaatca ggtgcccaca ttgtcgtggg aacaccgggt cgcttgttag atttgattaa
     3781 gcgcaaggct cttattttag atcatgtgga aaccttaatc cttgatgaag cagatgaaat
     3841 gcttaacatg ggattcttgg aagacattga agctattatt agtcgtgtcc cagcagaccg
     3901 tcaaaccttg ctcttctccg caacgatgcc ggcacctatt aaacaaatcg gggttaaatt
     3961 catgaaagac cctgaacatg ttcaaattaa gaacaaagaa ttaacaaacg tcaatgttga
     4021 tcaatactat gttcgtgtga aagaacaaga aaaatttgat actatgaccc gtctgatgga
     4081 tgttaaccag ccagaattat ccatcgtttt tgggcggact aagcgccgag ttgatgaaat
     4141 tacacgtggc ttgaaacttc gtggtttccg tgccgaagga atccatggtg atcttgacca
     4201 aaataaacgc ttacgtgtta tccgtgattt caaaaacgac caaattgata ttcttgttgc
     4261 cactgatgta gcagcgcgtg gccttgatat ttcaggagtt acccacgttt ataactacga
     4321 tattacccaa gatccagaaa gttatgttca ccgtattggt cgtacaggtc gtgccggtaa
     4381 atctggtgag tctatcactt ttgtctcacc aaatgagatg ggctacctct caatgattga
     4441 gaacttaacg aaaaaacaga tgaaacctct tcgtccagca actgctgaag aggcctttca
     4501 agccaagaaa aaagtagccc tcaagaaaat tgaacgcgac tttgcggatg agactattcg
     4561 ttcaaacttt gataaattca aaggcgatgc tgtccaattg gctgccgaat tcactcctga
     4621 agaattagct ttatatattt tgagcttaac ggttcaagat ccagatagct taccggaagt
     4681 tgaaattgca cgtgaaaaac cattaccatt caaatatgtg ggtggtggtc acggcaataa
     4741 aaatggcaaa ggtggtcgtg gacgtgacaa ccgtaaccgt ggtgatcgcc gtggtggcta
     4801 tcgtggtgac cgtaacagag atgagcgtga tggtgaccgt cgtcgtcaaa aacgtgataa
     4861 gcgtgatggt cacgatggca gtggcaaccg cgatttcaaa cgtaaatcaa aacgtaatag
     4921 taaagacttt ttcaataaag agaaaaaatc aagtgctaaa aatactggtt ttgtgattcg
     4981 tcacaaaggt gaataagctt aaagagagtt gatgagagtc agctcttttt aattttctga
     5041 aaaagttgct tttataatgg gatctgttat aatggtttca gttatttcat tataattttt
     5101 aaaaaattag gaggagtata agatgtctga tagccatctt actgcctttg acaaagcttc
     5161 aaaagcaggt tttattattg ctttaggtat tgtttatgga gatattggaa caagccccct
     5221 ttatacgatg cagtctctag ttgaaaatca aggaggggtt aatcaggttt ctgaatcctt
     5281 tattttagga tcaatctctt taattatttg gacactgacc ttgatcacaa caatcaagta
     5341 cgttttgatt gctctaaaag ctgataacca ccatgaaggt gggatttttt ctttattcac
     5401 cttggtacgt aaaatatcac cgtggctcat tattccggcg atgattggag gtgcaacact
     5461 tttgtctgat ggggctctta cacctgcggt aacagttact tcagctattg agggcttgaa
     5521 agccgttcct ggtctgagtc acatctacca aaatcaaacc aatgtcatta tcacaactct
     5581 agtgatttta atagtcttgt ttggtattca gcggtttgga acaggtttta ttggtaaaat
     5641 ttttggacca gttatgttta tctggtttag ttttctaggt gttagtggtt tttttaatac
     5701 gttggggcat ctggaaattt tcaaggctat caatccttac tatgctctgc atttgctgtt
     5761 tagtcctgaa aaccatcgtg ggatttttat cttggggtcc atttttcttg cgacaacagg
     5821 agctgaagcc ctttattcgg atttaggaca tgttggtcga ggaaatattt atgtgagctg
     5881 gccctttgtt aagatgtgta ttgtgttgtc ttactgtggc caggctgcgt ggattttagc
     5941 gaataaacac tcaggtattg aactaaaccc tttctttgct agtgtgccat cacaattaag
     6001 agtttacctt gttagtttgg caaccttggc agctattatt gcctcacagg ctcttatttc
     6061 aggctccttt accttgattt cagaggccat gcgtttgaaa attttccctt tgtttcgtgt
     6121 gacttaccca ggggcaaatt tggggcaatt gtatattcct gtcattaact ggattttatt
     6181 tgcagtgacc tcttgtactg ttttggcttt tagaacatca gcccacatgg aggcagctta
     6241 tggtcttgct attaccatca ccatgttgat gacaacaatc ctcttaaagt attacttaat
     6301 caagaaagga acgagaccca tcttggctca tttagcgatg gctttctttg cccttgtaga
     6361 attcattttc ttcttggctt cagctatcaa atttatgcac ggtggctatg cggttgttat
     6421 cttggcactt gcaattgtct ttgtcatgtt tatctggcat gcgggcacac ggattgtttt
     6481 caagtatgtg aaatctctta atttgaatga ttacaaggag cagattaaac aacttcgtga
     6541 cgacgtttgt tttgacctct accaaacaaa tgttgtctac ttatccaatc gtatgcagga
     6601 tcatatgatt gaccggtcta ttttgtactc cattttagac aagcggccaa aacgcgctca
     6661 agtttattgg tttgtaaatg tccaggtaac tgatgaacct tatacagcta agtacaaggt
     6721 agatatgatg ggaacggatt atatggttcg agtcaacctt tatcttggtt ttagaatgcc
     6781 acagactgtg ccacgctatt tgcgcacgat tgttcaggat ttgatggaaa gtggccgttt
     6841 acccaaacaa gaacaggaat ataccatcac acctggtcgt gatgttggtg attttcgctt
     6901 tgttttaata gaagaacggg tcaccaatgc acgtcaattg agtaactttg aacgctttat
     6961 tatgcagact aaggcaagca ttaaacatgt taccgctagt ccaatgcgtt ggtttgggct
     7021 tcaatattcc gaagtaaccc ttgaagtggt tcccttgatt ctatcagatg tcttgaagtt
     7081 accaatcaaa gaattggtgc ctgtggaaga cagtgaagca taaaaaaatc attgagatga
     7141 taagtctcaa tgatttttct tttctttttg atgagtagca atataggcca atttctggct
     7201 tcttgttttt cgttttttaa agagtgcttc agcgctcatc gcagcctctt tgctatcaaa
     7261 cacttcgtaa taaagtaaag aaacaggtaa ccggtagcga gtatatttag ccccctttcc
     7321 tgcgttatgt gtagctaaac gctttttcaa atcagttgta taaccagtgt agagggtttt
     7381 atctgcacat tctaacacat acatataagc ttttttagtg gtcatgatta ggttttcctt
     7441 tggaagcagc accaaaataa atctcaaaaa tatgatcagt gtattctccg ttttccttgt
     7501 ggacaactaa aggaggtaaa atagtcatcc cttcaataga accgtctttg attgcttcaa
     7561 taagtaacat attagctgat ttacctaatt taggatagac aaattgtacc cttttagggg
     7621 caagaccatt agctctaagg ctatctataa tttccaagaa tcgatctggt cgatgaacca
     7681 ttgccagacg accatttgat ttaagggcat gacgggctac ctgacaaatc tcttctaaat
     7741 tagtggtcac ttcatgacga gccagtaaat aatgttcaga gacgttcttc ttagaacttt
     7801 cgtgactttt aaagtaaggg ggattgcaga gcatcaaatc aactcctgaa cgtggcacat
     7861 ggtttaggag gttttttaaa tcatcacaaa tcatggttac ttggtcttcc agttgattga
     7921 gctggataga gcgttgtccc atatcagcaa gccgttcttg taattctact tcaacgatgg
     7981 ctgccttggt tctagtgcta gcaaataagc caaccgcccc atttcctgag cacaaatcca
     8041 caattaaccc tttggaaggc atcttgggga agcgggataa gaggacgcta tcaatagagt
     8101 aactaaagac atccttgttt tgaataatgc caacatcact tgaaaagagt tgatcaatac
     8161 gttcgccttc ttttaagatt gcttttatca tacctgttat tttaacataa aataggcttg
     8221 aggtgaatgt ttgaagagtg attttgactt tcgaaaagtc agctttcatg atacaataag
     8281 taagattatt ctattaaagg agaagcctgt gttttacgct tacttacgcg gtttagtagt
     8341 ctttttatta tgggttgtca atggcaatgc ccactaccat catgaagaaa agatgttaga
     8401 tgcttctgag aattatattc tggtagcacc ccatcgaacc ttttgggatc cagtttacat
     8461 ggcatttgca gcacgtccta agcagtttat ttttatggct aaaaaagagt tgtttgcaaa
     8521 tcgtctcttt gcttggtgga ttaaaatgtg tggtgctttt cctattgatc gtgacaaacc
     8581 gagtccagat gctattcgtt accctgttaa tatgctcaaa aagagcaatc gttctcttct
     8641 tatgtttcca agtggaagta ggcattcgca agaggtcaaa ggaggggtcg ctgtgattgc
     8701 taaattggct aaggtcaaga ttatgccagc agcctatcag gggcctatgt cggtcaaggg
     8761 attactagcg ggagaacggg ttgacatgac ttttgggaat cccattgacg tctcagatat
     8821 taaacgcatg aatgatgaag ggattgctga ggtggcaaac cgtattcagg cagagtttga
     8881 tcggatggat gacgagttgg cacctttcca accaggaaaa gcaaggaacc ctttaactta
     8941 tctctaccgt ttaccattag gattggtttt ggtggtggta ttattattaa ccatattatt
     9001 ttcctatatt gctagttttg tttggaatcc agataaacat cgccattaag aacatgaggg
     9061 ctaacatgtg cctgaaataa gaagtgattg agattagtct tctattgaaa gaagccgcta
     9121 atgcttaatc acttttttca tttctaaaaa agatattcga tgataccctt aactctttta
     9181 agcacaaaaa atgacaatac tactatactt ttggatatca tttacgaata aaaagatgag
     9241 gtaaaaaaat gttagacgaa ttattaatta aaggaaaatc gtttttgaga gagcggtatt
     9301 ttctgccata cctaattagt cttttattag gactttttct aatactaagc ttcagttttt
     9361 tgttctgggg aaataggcag agcaaggcag ccgttcctgc tttgagagaa atatcgcctg
     9421 tgaaacagca ggttagtgaa gaaaaaaagg aaattcaaga agatagcagt attttagtgg
     9481 atttaaaagg cgccgtacaa aaagagggag tttataagct aactgctagc agtcgtgtgc
     9541 gagatgtcat tgaactagct ggaggcctaa cttcagaagc tgataaacat gctattaatt
     9601 ttgcagaaaa actaactgat gagcaagttg tttatgtacc taaacaagga gaagagattt
     9661 ctgtgctacc aagatcatta gtttctggta aaaaagaaac tgccagcaag gatcagtcaa
     9721 aggttcatat taataaggcc agcttagaag aactacaaca tatcccaggt attggtgcta
     9781 aaagagctca ggatattatt gatatgagag ataagttagg tggttttaaa gcgttagaag
     9841 atctccgtca ggtttcgggg attggcgaaa aaacgctcga gaaattaaaa gatgacattt
     9901 tcttggacta agctagtgcc cctgtcaaag attcaatttg cttttttaat cttggttctc
     9961 ttttatcaaa tccatagccc tagctggtta acgtttctcc ttagccttag tttgatttgc
    10021 ttacttgtca agcgtttgtc taaaaaagaa tttctcggtg tctttgccat ccttagtttc
    10081 tgtgctttgt ttttgctgta tcagaaacag cagttgattc aaaaactgga aattcagcca
    10141 gtacaaataa cctctgtagc attagttcct gatagtattc ggattaatgg tgatcaatta
    10201 ctgttcttgg acgtcacggg aaacattctt atcagttatt ttaccgtttg aaaagccaag
    10261 cagaagccca attgttcaag aaagagcacc gttggttagt gatgcacgca aaagtgacat
    10321 tggaaaaagc tgaggaagtt cgaaatttta aaggctttaa ttatcagact tttttggctt
    10381 atcaaggaat ttacaggata gggaaggtag agcaaataga acagcttgaa gtgatttcac
    10441 ccgagtcaat atgtgactat ttgtcctctt taagacgacg agccattgtt cattgccagc
    10501 agcattttcc aagacctatg tctcactacc tgacgggcct cctttttggt tatttagata
    10561 agtcgtttgg tgagatgaca gattattata gtcagttggg tatcattcat ctctttgctt
    10621 tatcagggat gcaggttggc ttctttctta cttgttttag aagggtgttg ctattactcg
    10681 cagttccttt ggaatggata aaatggatag agcttccttt tgcttgcttt tatgccgcgt
    10741 tgacaggtta tagtatttct gttattcgaa gcctcgtgca gtcgcagttg cgtcatttag
    10801 gaataaaagg attagacaat ctggcttgta cttttttact tgtcttttta tgggatgctc
    10861 atttcttaat gactgttggt ggcgtattaa cgtttagcta tgcttttttg ttaacagtgg
    10921 ttactgttga agaattatca ggagctaaaa gacagttggt acaagtcttg accatttccc
    10981 taggaatatt accatttttg ctcttttatt tttctagttt taaccccatg tcaatggtct
    11041 taacagggct cttgtcttat ctctttgacc tttttatttt gccccttctt tgtctggttt
    11101 tttgtctatc ccctttggtg actgtctcaa tatgtaacca tctttttatt ttattagaaa
    11161 aagtcattca atttctcgga aatactttca atagttccct tgtttttgga agcccgacca
    11221 gttggcactt gcttatcttg gtgattagct tcgctatttt ttatgattat cggcaggtgc
    11281 gacaacgagt tatcacttgc ggtttggtta tagcgttgac gcttttgtct gtcaaatatc
    11341 ctttgaccaa tgaagtgacc tttatcgaca ttggccaggg agatagtatt ttggtgaggg
    11401 agtggacagg gaaaaatctc ttaatagatg taggtgggcg ccctttcttt tcttcaaaag
    11461 agcactggcg acgaggacat cacgtagcta atgctcagaa aaccttaatt ccttacttaa
    11521 aaagtagagg tattcacaca attgaccaac tattagtaac tcacgcagat actgatcata
    11581 tgggggatat cgaagtggtt gccaaagcta ttcgaataaa ggagattttg actagtcaag
    11641 gcagtttaag ccatccaagc tttgttaggc ggctaaggcg tttaaaatgt cacgttaggg
    11701 ttttagcagc tggagaccag ttgcctatta tgggaagtgt cttacaggta ctctaccctt
    11761 ggcagttagg ggacgggaaa aataacgatt ctttggtact ttatggcagg ctcttaaatc
    11821 gaacttttct tttcacaggt gacttggaaa aagaggggga aaacgagatc ataaaacgtt
    11881 atcctcaact aagagtagat tatttaaaag caggtcacca tggatcaaat acctcttcta
    11941 gtgcagcttt tttagaccat attcagccta aagtggcctt tatttctgct ggaaaaaata
    12001 accgttatca gcatcctcac agagaaacct tagcccgttt ggaagacaga caaattactt
    12061 attaccgaac ggatacgcaa ggagctattc gtttgactgg ttggacaagc tggcatcttg
    12121 aaacggtccg ttagttaaaa ttccagcctg acgaagaaac aagcagtcgc tttcctttta
    12181 ggagaaagat gatataataa aagcatgatt gcgatagaaa agattgaaaa actgagtaaa
    12241 gaaaatttgg gtcttataac ccttgtcaca ggagatgaca ttggtcagta tagccagttg
    12301 aaatcccgct taatggagca gattgctttt gataaggatg atttggccta ttcttacttt
    12361 gatatgtctg aggccgctta tcaggatgca gaaatggatc tagtgagcct acccttcttt
    12421 gctgagcaga aggtggtcat ttttgaccat ttgttagata tcacgaccaa taaaaaaagt
    12481 ttcttaaaag aaaaagacct aaaggccttt gaagcctatt tagaaaatcc cttagagact
    12541 actcgactaa ttatctttgc tccaggtaaa ttggatagta agagacggct tgttaagctt
    12601 ttgaaacgtg atgcccttgt tttagaagcc aaccctctga aagaagcaga gctaagaact
    12661 tattttcaaa aatacagtca tcaactgggt ttgggatttg agagtggcgc ttttgaacaa
    12721 ctgcttttaa aatcaaacga tgattttagt caaatcatga aaaacatggc ttttttaaaa
    12781 gcctataaaa aaacgggaaa tattagccta actgatattg agcaagccat tcctaaaagt
    12841 ttacaagaca atatttttga cttaaccaga cttgtcctag gaggtaaagt tgatgtggct
    12901 agagatttga ttcatgattt acgcttatca ggggaagatg atattaaatt aattgctatt
    12961 atgttggggc aatttcgctt atatttacag cttgccattc ttgctaaaga aggaaaatca
    13021 gaacagcagc ttgtaaccag tttgtcagag atacttgatc ggcgggttaa tccttaccag
    13081 gtcaagtatg cgttaaagga ttctaggacc ttatctcttg cctttctaac aggagcggtg
    13141 aaaaccttga ttgagacaga ttaccagata aaaacaggac tttatgagaa gagttatcta
    13201 gttgatattg ctctcttaaa aatcatgact cactctcaaa aataggcgtt ggaccgcttt
    13261 ccaagagata atcaaattag ttgaaagctc ttattttttg cgataagata gacgtatcat
    13321 aaaggaaaag aggacaggaa aatggctatt attttaccag aacttccata cgcgtatgat
    13381 gctcttgaac cacagtttga tgcggagaca atgactcttc atcatgataa acaccatgcc
    13441 acttatgtcg ctaataccaa tgcggcttta gaaaaacacc cagagatcgg agaaaatctg
    13501 gaggaactat tggcagatgt cactaagatt ccagaagata ttcgtcaagc cttaattaat
    13561 aatggtggcg gacacctaaa ccatgcgctt ttctgggaac tattatcacc tgaaaaacaa
    13621 gacgtcactc ctgatgttgc acaagccatt gatgatgctt ttgggtcatt tgacgccttt
    13681 aaagaacaat ttacagcagc tgctactgga cgttttggtt caggttgggc atggttagtt
    13741 gttaataaag aaggacaact cgaaatcaca tcaaccgcta accaagatac tccaatttca
    13801 gaaggcaaaa aaccaattct agcgcttgat gtgtgggagc atgcttacta cttgaattat
    13861 cgtaatgtac gtccaaatta tatcaaagct ttctttgaaa tcattaattg gaaaaaagta
    13921 tcagaacttt accaagccgc taaataagaa aagggtttcc aatctcaaaa agagattggt
    13981 tttttaagtg gttttgtgaa taacagtgtg gggacatcat tttttagaaa agataaaaat
    14041 agtttgtctt tttaatgtaa ttccttgatt tttagctaaa tttagctaga atagaatgga
    14101 taggagattt taggtgttaa gaaagaagaa aactaccatt gtagggatta ttttaggaat
    14161 gatgatagtg accttacttg tcggctgttt gactggtaaa aagaaaggct ttcctcctaa
    14221 agcaggtgag cggcagcaaa cggctgactt aaaaatgaaa aaagattaga aaaactgttc
    14281 aaaaaccgtt acctgaaaaa tctaaagagg ctaagcaagc tgttatggat gcagatttag
    14341 cgcaaatgga tgccttaggg ctctattacg atgacaacca tttgagtttg gtcaatacgg
    14401 tcaaggtgta tttaaaggaa tttggcattg gtgaatctca aattgccttt tcctacaaag
    14461 acctcaattc aggcaggact gctgccatga atgaccatca gccaatgatt gcaggaagca
    14521 cctataagct gccattaaac atgctggtgg tagataaggc aaattatcca tgacagagcg
    14581 ttttgacatt acagaaactg attatgaata tgatcaggaa catcatgcct atgttgctca
    14641 attcaatggt gccatgtcta ttccggatat gcaagaatat tccttggtgt actcagaaaa
    14701 cacgccagct tatgctctag cagaacgatt gggtggcatg aataaggctt accaattatt
    14761 tgaccgctac gggaaggtat ccggagctat tacgaccatt gatcgtaatg gcaataaaat
    14821 caccaccgct tactatttgc aaggtttgga ttacttgtgg cagcatcagg ataaatataa
    14881 ggatatcttg tactacattg gtgaatcttt ccctgatttg tattacaaga cttatttgcc
    14941 tcatgtaaag gtttatcaaa aaccgggtta tgtcagagaa gccttaaatg taggtgccat
    15001 tgtttgcgaa gagtctcctt atttgattac tctttattca tctggtttag gtggagcaac
    15061 acaagcatca gaggaagtca atggccttgg ttatgtgcag cttgttcagc taacatatgt
    15121 aattaatgag tggcatagag tcaatcacaa catgatctaa tactcagttg acattagtct
    15181 gatagtagac atacttactg atgaaagtca ctaaaataag tttttgctca ttaaaaaagc
    15241 ttggcctgtt tcttcaggtc aagctttttg agattaatgc tgtgtttttg aggtcttatt
    15301 gtcacctctt gtttcttgat aatggtgaag caattttttg aattttttaa cggcagcgcg
    15361 tgttagctgc ttagatgatt tgccatttct gctgtaaatg ggattgtgct tatccctttg
    15421 ctgttctaaa tcgggaacta gaaaagttgc atcaggacta ttaattgctt tttcaataaa
    15481 agccggatca aaaagcagaa tagggtcaat atcaagcaga cggtgcaagt catcgttttg
    15541 gttgccataa ttgaaagaag aaccattgac aatactattt cgcgttaggt tatcaggatc
    15601 tagtgctact aaaaattgtc cagattctga caaaataaac tctgagtgaa aatgaattaa
    15661 aatcttatag ttggcttgag gaatattgtc tgtatagact gcctgatgat tgccattagc
    15721 atctaaaata ctagcaacct tattatggta tctagaagat tcataaagag cataatcgtc
    15781 atcttctaga gtagccaaat atttggctaa ggcatccgca tcggttttcc ctttattccc
    15841 ataatgtgag cgaacccact ccgtttgctg ggttgatatg aggtaacgga gttgatgaat
    15901 tttccgatta aaacggctat aaggaatgtg ttcgagatta acataataag cctgaatcag
    15961 ggtagataat tggtcccaaa aaggatctgt tggcgatagc tttggactta attgtgccaa
    16021 tacttgctgt gtttgttttg attgtcctgt caactcgtct ggcatcttta aatacgtcaa
    16081 gagcagttgt aaccgctttt gagcttcttg aggcgtctgt gccatctctt cttgttgcca
    16141 aagcttttga aagtcaggtg atcctatcag gcgaatgcga ctataaagtt gttcgaaatg
    16201 ataagagttt cctctaacag cgagttgttc aaggtatcct ttttggatgg ctttatagga
    16261 ccatccgtcc ttatcgagct ttcgaacata atcaagcaag tcgtggcgta atttaatagc
    16321 ttctgaaagc tggagttctc gtaatttagg atagttttgg taaaaaggtg ccactaaatt
    16381 ttgataaagt ttgtgttctt tttcaaaggc aatccgctga tagtctttct gccaaaaact
    16441 gacgcaaaaa gtcagaaaca tcaagaagaa tagccatttt ccaagtaatt tcataggttt
    16501 ttagttgaca aacatggcat cgccaaagct aaagaaacga tacttttcgt caaccgcgtg
    16561 tctgtaagct tcaaggacaa agtcacgccc tgcaaaggct gatactagca tcacaagggt
    16621 tgattttgga agatggaaat tagttgaaaa agcatcaact accttgaatt ggtagcctgg
    16681 tttaataaaa atattggtcc aacctgaatc agcttgaatg tccccttgga acttacctcc
    16741 gatggtttcc aacgtacgaa tagaggtggt tcctacagcg acaacacgac cgccagcttg
    16801 tttgacgtct cttagggttt gcgctgcttc ttctgagagg gaataaaatt ccgaatgcat
    16861 atcatgctca tcgaggttat ccacggaaac aggtctaaaa gtcccaagcc ctacatgtag
    16921 ggtcaaataa accaaatgta cccctttggc ttcgattttt ttgagcaagt ctgttgtgaa
    16981 gtggagacca gcagttgggg cggccgcaga gccattttct ttagcataaa cggtttggta
    17041 tcgctcagca tcctctaatt tttcgtgaat ataaggaggc aatggcattt cgccgaggct
    17101 ctctaaaact tctaagaaaa tcccatcata gctgaattca acaattcgtc caccatgttc
    17161 taattcgtcg ataatggtgg ctttgagacg cccatctcca aaattgactt ggctaccaac
    17221 cttgagccgt ttggctggct tggctaaaac ttcccattga tctccttgtg tatttttaag
    17281 caaaagcaat tccacatgac catgagtatc gggtttttct ccataaagtc tggcaggcaa
    17341 gacacgggtg ttattcatga cgagggcatc ccctggattt aattgatcaa tgatatggtc
    17401 aaaatggcta tcaaccatag ttttttgacg gtgatcaata atcaatagtt ttgaactgtc
    17461 tcttttttcg agtggggttt gggcaatcaa ttcttctggt aactcaaaat caaaatcgtt
    17521 agtattcatt gggactcctt taaagccact agtaaatctc ttcctagtaa ctctttcatg
    17581 taagataaat cttttctatt atatcacttg tcgtctaacc cgtcatcttt aatctttcat
    17641 cagaaagtaa aggataggct tgacaaaaat tggtatatac catataataa aaacacgaaa
    17701 ggtctatacc aattagaaaa ggagaatgac atgaaaatta ttcgggtcca agatcagatt
    17761 gaaggtggga aaatcgcttt taccttattg aaagatagct tagctaaggg agctaagacg
    17821 ttaggattag caacaggaag ttcaccaatt agtttttacc aagaaatggt caaaagtcct
    17881 ttggattttt cggacttaac aagtattaac ttggatgaat atgtcggtct ttcagtagag
    17941 agcgatcaga gttatgatta ttttatgcgt caaaaccttt ttaatgcgaa gccttttaaa
    18001 aagaattact tgcccaatgg tttagcaact gatatcgaag ctgaagctaa acgatatgac
    18061 caaatcattg cagagcatcc gattgatttt caagtgcttg ggattgggcg taatgggcac
    18121 attggtttta acgaacctgg gacctctttt gaagaagaaa cgcatgtagt ggatcttcag
    18181 gaatcaacta tcaaagccaa tagtcgtttc tttacaagta ttgaggatgt gccaaaacaa
    18241 gctatctcta tgggaattgc ctccatcatg aagtccaaaa tgattgttct tcttgccttt
    18301 ggacaagaaa aagctgatgc gatcaaggga atggtttttg gaccaatcac agaagacctc
    18361 ccagcaagta ttctccaaaa acatgatcat gttatcgtca ttgtggatga agctgctgcg
    18421 tcacaattag attaacctaa ccagtcgtta aaccttgtaa gaacaaaaat gaggagatag
    18481 tcaacctcat ttaagaagaa gtcttgtaac ttctatttct tttgcaaggg taaatttatg
    18541 ttacactact aatatgcgtt tagataaatt attagaaggt acaaaagttg gctctagaag
    18601 tcaagtgaag aaactgatta aggctcaagg ggtttgggtt gatcacatgc ctgccagaaa
    18661 tggtcgacaa aatgttgatc ctggcttgca actgattgag gtgactggac agcgagtgac
    18721 gcatcctaaa cattcctaca ttatcctcaa caagccatca ggggtggtct cagctaagaa
    18781 agatactaac tatttaaccg ttattgatca gcttgctgaa gaagataaaa gtccagactt
    18841 gtatcctgtt ggcagattgg atagagatac cgaagggttg gttttactca ctgataatgg
    18901 tcccctaggg tttcggatgt tgcaccctat tcatcatgtt tctaaaacgt atcttgtaac
    18961 cgttaatggc ttgttagcag aagatgcctc agattttttt gcagctggta tttgtttccc
    19021 aacaggtgaa caatgtcaac cagctcaatt gactattctt aaagccgaca cagaccagtc
    19081 acaggctagt ttgaccattt cagaaggaaa atttcaccaa gtgaaaaaaa tgtttttgac
    19141 ctatggtttg aaagtgacct ctctgaaacg gacgcacttt gcaggattgg aactcggtca
    19201 tttagccagc ggagaatatc gttatctaac agcgtcagaa aggcaactca ttaaaacgta
    19261 tctagactga gcaaaacatg ctttcaaaaa gaagctattc ttgcttaatc aataaagtgt
    19321 ccattgacta ttatagtcat gatgagtccg acttttgcta tgtttagtca tcttgttgtt
    19381 acaaaatcag gtaagcaaaa aaattcaatt cccactgtct ccttaacctg aagcgttata
    19441 aaaacccttg tgttaaactc tcataaattg gaaatgggag tgttttttga tggctaaaaa
    19501 aactaaagct acttaaagtt tggtacttag ttttcgaata ataaggtgga agatctaata
    19561 ggaggtgaca aaaatattaa ccgctttaga tggtaagaat cagctcattt cgctagtaac
    19621 tcagcctatt tcaacgaaac cgcctttttg ttgcccagct tgcaaatctc ctgttcgctt
    19681 gcgtcagggg acaattagac gacctcattt tgcccatgta caactagctc attgtcaatt
    19741 ccaagcagaa aatgaatctg aggaacattt gacgttgaag gcaaaacttt acaccagttt
    19801 agtccgaaca gaagctgtct gtattgaaaa atatcttcct gagctgcaac agatagcaga
    19861 cctttgggtg aatgataagt tagccttaga aattcagtgc agtcccttgc cagtggagcg
    19921 tctaaagaaa cgcaccaaag cctatcaaga aaaggggtat ccggtacgat ggttactggg
    19981 aagaaaattg tggcttaaca ctcacttaac tgctttgcaa aaacagtttc tttacttttc
    20041 atctagtttg ggctttcatt tgtgggagct ggatgcagct gcaaatctgc tacgtctcaa
    20101 atatttaatc catgaagact tatttggtaa ggtaagttat ctaaccaaaa ccatttcttt
    20161 agaccacaat attatggaaa tgtttcgatt accctatcag caagaaatcc tttattctta
    20221 tcagaaaaaa atgactgtga acctatctaa aagaatacaa agagctcttt tagctagaca
    20281 cccgaaatgg ctacgacgac aagaaaaggc ttacttatca ggctataatt tacttatgtt
    20341 aacaactgat gctttttatc ctcaatggag accagttcaa tcttccagcg gtttttgtca
    20401 aattaaagga aatcttcgcc cttattatga aagttttaaa gtctattata aaaaagaaaa
    20461 ggataagaag gtgcaaacgc ttttttcacc aaaatattat gttaaaatgg atagtaatag
    20521 aaaatgagaa ggagatgtta tgactgataa tcgcagccat ttagaagaaa aatacacttg
    20581 ggatttgagc actatttttg caacagataa ggattgggaa gctgaagtca gtgacttagc
    20641 tactgaaatt gacgcggcta aggatttttc aggtcattta ctagattcta gcgctaatct
    20701 gttgacaatt acagatactt atttagagtt agctcgtcgt gttgagaaag tctatgttta
    20761 tgctcacatg aaaaatgacc aagacactac tgttgccaag tatcaagaat accaagctaa
    20821 ggcttcaggc ttgtatgcaa agtttagcga agtgttttca ttctatgacc cagaagttat
    20881 ggcattgagt caggaagatt accaagcttt tcttgcggaa acacctgatt taaaaaggta
    20941 tcatcatttc tttgacaaat tgttccagac acgtgaccat gtgctcagcc aggctgaaga
    21001 ggaattatta gcaggagccc aagaaatttt caacggcgct gaagaaacct ttagtattct
    21061 tgataacgct gatattgtct ttccagtagt taaaaatgat aagggggaag atgttgaatt
    21121 aacccatggt aacttcatta gcctaatgga atctaaggac cgtagcattc gccaagctgc
    21181 ttatgaagcc atgtatagta cttatgaaca gttccaacat acttacgcta aaaccttgca
    21241 aaccaatgtg aaagtgcaaa actataaggc gcgtgttcat aaatacgatt cagcacgtca
    21301 agctgccatg gctgctaact ttattccaga agctgtttac gataccttgt tagaaacggt
    21361 taataaacat ttgccactct tacaccgtta tttgaaattg cgtcaagagg ttcttggttt
    21421 agacgatttg aagatgtatg atgtttacac accattatct gaaacagatt tagccattgg
    21481 ttatgatgag gctttggaaa aggctgagaa ggttttggct gtttttggta aggattacgc
    21541 ggatcgtgta catcgtgctt ttaccgaacg ttggattgat gttcacgtcg ataaagggaa
    21601 gcgctctggg gcttattcag gtggttccta tgataccaat gccttcatgc tgttgaactg
    21661 gcaagataca cttgataatc tttacacact tgtccacgaa acaggccaca gtttgcattc
    21721 tactttcact cgtgaaacac agccttatgt gtatggggat tatagtatct tcttggcaga
    21781 aattgcatcc acaacgaatg aaaacatcat gacagaagct ctcttaaatg aagtgcaaga
    21841 tgagaaagaa cgctttgcca ttttaaacca ttacctcgat ggtttccgtg gaacagtttt
    21901 ccgtcaaacc caatttgctg agtttgaaca tgccattcat caagctgatc aaaaaggaga
    21961 agtgttaaca agtgaatacc ttaaccagct atacgctgat ttaaatgaaa aatattatgg
    22021 tttaagtaaa aaagataatc attttattca gtatgaatgg gcacgtattc ctcatttcta
    22081 ttacaattat tatgtttacc aatatgcaac agggtttgca gctgcaagct atttagcgga
    22141 taagattgtg catggcacac aagatgatat cgatcattac ttggcctacc tcaagtcagg
    22201 aaattctgat tacccacttg aagtgattgc taaagcaggc gttgatatgg caaaaggtga
    22261 ttatttggaa gctgccttta aagtctttga cgaacgtcta acagaacttg aagtactcgt
    22321 gtccaaaggc atacacaaaa gctaaaatca caaaagtcaa ggacaaaaag agtttaggat
    22381 tgatagaaat ggagattcac agttggtgtg aatctccatt tctatcggtc attttctttt
    22441 tatattttag tggattatca gtcctgaaaa gcaggcttca ttgaccttag tttgacggtt
    22501 tcttttttgt cttttttatt gtgtggtcac tcacttaaat aaaaccaata gttgagtaag
    22561 gcgcttcaga ttaacgccat tccattgttt tagagataac gttcgtataa aacgtgtttt
    22621 tattatattt gacattttat aaaagagata atagaattaa aatactaaca tttatgtttt
    22681 ttaaaaaata aacgttcgta taataaaaaa ggagtttttt atggaaaaaa caaaacgtta
    22741 tatcattgca acagcaggga tacttctcca cttaatgttg ggatcgacct atgcttggag
    22801 tgtttatcgt aatccaattt tacaagagac cggttgggat caagctcctg tcgcttttgc
    22861 cttttcttta gctatttttt gtttaggttt atctgctgct tttatgggaa atttagttga
    22921 acaatatggt cctcgtctaa ccggaacggt gtcggctatt ctttatgctt ctggaaatat
    22981 gctgactggt ttagcgattg atcgtaaaga aatttggctt ttatatattg gttatggtgt
    23041 tattggtggc ttaggacttg gagcgggata tatcacaccg atatcaacta ttataaaatg
    23101 gtttccagat aaaagaggca tggcaacagg gtttgctatt atggggtttg gttttgcgtc
    23161 tttattaaca agtccgattg cccagtggct aattgaaaca gaaggtttgg tagcaacatt
    23221 ttacctttta gggctgattt atctaatagt tatgcttttt gcttctcaat tgataataaa
    23281 accaactgct gctgagatag ctatactaga taaaaaaaga ttacaaaata acagttattt
    23341 aatcgaagga atgaccgcta aagaagcact taaaacaaaa tcattttatt gtctttgggt
    23401 aattttattt atcaatatca catgtggctt aggacttatc tcagttgttg cgccaatggc
    23461 acaagatcta actggtatga gtccagaaat gtcagcaatt gtggttggtg ctatggggat
    23521 ttttaacggt ttcggtcgtt tagtgtgggc cagtttgtca gactatattg gacgccgtgt
    23581 gacagtaata cttttatttt tagtaagcat aattatgact attagtttaa tatttgcaca
    23641 tagttcccta attttcatga taagcattgc cacccttatg acttgttatg gtgcgggttt
    23701 ttctctcatt ccgccttatt taagtgatct ttttggggca aaggaactgg ctacattaca
    23761 tgggtatata ttgacagctt gggcaatagc tgcattaact ggacctatgt tattatctat
    23821 cactgttgaa tggactcata attatcttct taccttatgt gtttttattg tcttatacat
    23881 tttaggatta atggtagcat taaggttaaa gaaatgatgt cctataaaaa atttaaaagt
    23941 aaaccctagc aaagcctagg gtttttttat ataattaggt ttatggttaa atcatacagt
    24001 aaaacagcaa atcataatat gcgtcgtcct gttgtcaaag aagagcttgt tcattatatg
    24061 cgaacacggc agaagcaaat cacaggtttt ttagcagagt tagagcaatt cgcaagacaa
    24121 gaaaacattc ctattattca gcctgaggtc gtggcttatt tcaggttttt attgcaaagc
    24181 ttacagccaa aacacatttt ggagattggc acagccattg gtttttctgc gcttttaatg
    24241 gctgaaaatg caccagatgc gacgattgtg actattgatc gtaatcgaga gatgatcgat
    24301 tttgctaaag caaattttgc taagtacgac agtcgccaac agatccgcct gttagaagga
    24361 gatgcagcag atatattatc tacacttgaa ggaaattttg actttgtgtt tatggattcc
    24421 gccaaatcca aatatattgt ctttctacca gagattttaa gactgttaaa agtcggtggt
    24481 gtagtcattt tggatgatgt ctttcaagga ggtgacatta caaaaccgat cgaagacatt
    24541 cgacgaggtc agcgtactat ttatcgtggc ttacagtctt tgtttgatgc taccttaact
    24601 catccaaacc taacaactag cctggtccct cttagtgatg gattattaat gattcgtaaa
    24661 aatcaagcag atattctatt accagattaa gtaacattga taaatttatg ttataataaa
    24721 ttagttaaag ataaaaagga gctcagaagt aaatgaaaaa ctcaaataaa ctcattgcta
    24781 gtgttgtgac attggcctca gtgatggctt tagcagcttg tcaatcaact aatgacaata
    24841 ctaaggttat ttcgatgaaa ggtgatacaa ttagcgttag tgatttttac aatgaaacaa
    24901 agaacacaga agtatcgcaa aaagcgatgc taaatctggt aattagtcgt gtttttgaag
    24961 ctcaatatgg tgataaggtt tcaaaaaaag aagttgaaaa ggcgtatcat aaaacagctg
    25021 aacagtatgg cgcttcattc tctgctgctt tggcacaatc aagcttgaca cctgagactt
    25081 ttaagcgtca gatccgctct tcaaaattag tagaatatgc ggttaaagaa gcagctaaaa
    25141 aagaattgac aacacaagaa tataagaaag catatgaatc ttatactcca acaatggcag
    25201 tcgaaatgat tactttagat aatgaagaga cagctaaatc agtcttagag gaactaaaag
    25261 ccgaaggcgc agactttaca gctattgcta aagaaaaaac aacaacacct gagaaaaaag
    25321 tgacctataa atttgattca ggcgcgacaa atgtaccgac tgatgtcgta aaagcggctt
    25381 caagtttgaa tgagggtggc atatcagacg ttatctcggt tttagatcca acttcttatc
    25441 aaaagaagtt ttacattgtt aaggtgacta aaaaagcaga aaaaaaatca gattggcaag
    25501 aatataagaa acgtttgaaa gctatcatta tagctgaaaa atcaaaagat atgaatttcc
    25561 aaaacaaggt tattgcaaat gcattggata aagctaatgt aaaaattaaa gacaaagctt
    25621 ttgctaatat tttggcgcaa tatgcaaatc ttggtcaaaa aactaaagct gcaagtgaaa
    25681 gttcaacaac cagcgaatca tcaaaagctg cagaagagaa cccatcagaa tcagagcaaa
    25741 cacagacatc atcagctgaa gaaccaactg agactgaggc tcagacgcaa gagccagctg
    25801 cacaataagt aggattttat tgactaatca gttaaaaaaa cttataataa tttagttaag
    25861 aattgtttat caaggattag atacagaaaa gtgacggttg ctgcgagtca ttgataatta
    25921 gataaacatg ctacttaact ttacaacaat tgatatcgta aattaagaat gacaagttca
    25981 ttgaatagag gtggtaccgc ggttttcgcc ctctgtgaga tggacttgtt ttgtattgag
    26041 gactatttga aatcaacgaa aggaatagag tagactaggc tgccaggctt atagctgatc
    26101 tcctagcaag aaagacgagc tgtgtcaact ggtgtgatta cattactagc ttcagcataa
    26161 cactgttttt cttactcttt gtatctaaat ttttatgaaa gaattatcgt ctgcacaaat
    26221 ccgccaaatg tggttggatt tctggaaatc taaaggacat tgcgttgagc cttcagctaa
    26281 cttggttcct gtgaatgacc caacgcttct ttggatcaac tcaggtgttg caaccttgaa
    26341 aaaatacttt gatggttcag tgattccaga aaatccacgt attaccaatg cacaaaaatc
    26401 aattcgtact aatgatattg aaaatgttgg taaaacagca cgtcaccata ctatgtttga
    26461 aatgcttggt aacttctcaa ttggagacta tttccgtgat gaagctattg agtggggatt
    26521 tgaactcttg acaagtccag actggtttga tttccctaaa gataagctct acatgactta
    26581 ttacccagat gacaaggatt catataaccg ttggattgct tgtggcgttg aaccaagtca
    26641 cttggtgccg atcgaggata acttctggga aatcggtgct ggtccttcag gtccagatac
    26701 ggagattttc ttcgatcgtg gtgaagattt cgatccagaa aatatcggac ttcgcctctt
    26761 ggctgaagat atcgaaaacg atcgttacat cgaaatctgg aacatcgttc tctcacaatt
    26821 caatgctgac ccagccgtac cacgttcaga atacaaagaa ttaccaaaca aaaacattga
    26881 tacaggtgct ggtcttgaac gtcttgcagc tgttatgcaa ggggcaaaaa caaactttga
    26941 aactgacctc ttcatgccaa tcatccgtga agtagagaag ttgtcaggta aaacttataa
    27001 tccagatggc gacaacatga gtttcaaggt tatcgctgac cacatccgtg cgctttcatt
    27061 tgctatcggt gatggtgcgc ttcctggaaa tgaaggtcgt ggttacgttc ttcgtcgtct
    27121 tctccgtcgt gctgttatgc atggtcgtcg tcttggcatc aacgaaactt tcctttacaa
    27181 attggttccg actgttggac aaatcatgga aagctactac ccagaagtgc ttgaaaaacg
    27241 tgattttatc gagaaaatcg ttaaacgtga ggaagaaaca tttgctcgta ctatcgatgc
    27301 aggtagcggt cacttagatt cattgcttgc gcagcttaag gctgaaggta aggatactct
    27361 tgaaggtaaa gatatcttca aactttatga tacttatgga ttcccggttg aattgacaga
    27421 ggaattggca gaagatgcag gctacaagat tgatcacgaa ggctttaagt cagccatgaa
    27481 agaacaacaa gaccgtgcgc gtgcagctgt tgttaagggt ggttcaatgg ggatgcaaaa
    27541 tgaaacccta gctggtattg ttgaagaatc acgattcgaa tacgacacat atagtcttga
    27601 atcaagtctt tcagtcatca tcgctgataa tgaacgtacc gaagctgttt cagaaggtca
    27661 agctcttctt gtctttgctc aaacaccatt ctatgctgaa atgggtggac aggttgctga
    27721 cacaggtaga atcaaaaatg ataagggtga cacagttgct gaggttgttg atgttcaaaa
    27781 agcaccaaat ggtcaacctc tacacactgt aaacgttttg gcatcacttt cagttggaac
    27841 aaactacaca cttgaaatca acaaagagcg tcgtttggct gttgagaaaa accacacagc
    27901 aactcacttg cttcatgcag cccttcacaa tgttatcggt gaacacgcaa ctcaggctgg
    27961 ttcattgaac gaagaagaat tcttgcgctt tgattttact cactttgaag cagtaagcaa
    28021 tgaggaactt cgtcacattg aacaagaagt taatgagcaa atttggaacg ctcttacgat
    28081 cacaacgact gaaactgacg ttgaaaccgc aaaagagatg ggagcaatgg cactttttgg
    28141 tgagaaatat ggtaaagtgg ttcgtgtggt tcaaattggt aattattctg ttgaactttg
    28201 tggtggaact cacttaaata attcttcaga aatcggtctc ttcaagattg tcaaagaaga
    28261 aggtattggt tcaggcactc gtcgtattat tgcagttact ggtagacaag cttttgaagc
    28321 ttatcgtaac caagaggatg ccctaaaaga gatcgctgct actgtaaaag ctccgcaatt
    28381 gaaagatgca gcagctaaag tacaagctct tagcgactca cttcgtgatc ttcaaaaaga
    28441 aaatgcagag cttaaagaaa aagcagcagc tgcagcagct ggtgatgtct ttaaagatgt
    28501 tcaagaagct aagggcgtgc gcttcattgc tagtcaagtt gatgttgcag atgcaggggc
    28561 acttcgtaca tttgctgata actggaaaca aaaagactac tctgatgtgc ttgttctcgt
    28621 agcagctatt ggtgagaagg ttaatgtcct tgttgcaagc aaaactaaag atgtccacgc
    28681 tggtaacatg atcaaagaat tggcaccaat tgtagcaggt cgtggtggag gtaaaccaga
    28741 catggctatg gcaggtggta gcgatgcaag taaaattgca gagctgctag cagcagttgc
    28801 tgaaacagtg taaatccata aaaaaccaag ttatttagaa cttggttttt tatatttgaa
    28861 atgactacca tcataacctt tttgactatt atcgttgtca aaaagaaaaa gatatgttac
    28921 aattatagta acaaatagac atgaggaggt gttagagaaa atgacacatg gtatcaaact
    28981 taagaggcag atttgcttat gggcaagtat cctgactctt ttcattttaa gttatcatct
    29041 tatcagggta atctggtttg gagtaccctt gagttatctg gttttgggct ttacaggact
    29101 agccttgctg ttgaatactt tttcttattt tagaaagtaa gttataagtt aaagaagaat
    29161 gaaggagatg gcaatggagg ttattcttaa aaatcggctc aaagaattac gagccagaga
    29221 tggaattaat caaacggaaa tggctaaact ggcaggagta tcgaggcaga ccatcagtct
    29281 cattgagcgt aacgagtaca ccccatcagt tataattgcc atgaaaattg caaaggtatt
    29341 tcaggaacca gttgaagagg tgtttcgtct agtggaggtc gaggaatgaa aaagaagaag
    29401 aatagttttt tacgcttgtt aaaaatgtta ttgctatcaa gtttggcggg agggattata
    29461 ggtggcatgg ttggagcctt tttaggttac catggagaga gattagatca gctcacgttc
    29521 ctaaaagatg atgtgattaa cctgattatc ttattaaatc gtctagtagt tgtcacaggc
    29581 ctcacacttt cctttgtttt tttaacgcaa ctgaaaaaag agacagctgt ttataacaca
    29641 attgaagagg atgattattc tgaaaatggt taccgtcagc tgaataaaaa gcatgcttat
    29701 accatgttat tgattgccgt agcttctatt ttatccatgt gcaatgtgct acttggtctg
    29761 accttaacta atgattcaca gcatgccatg ctggccattc cgcttctgga tatcctttta
    29821 ttattaatgg tcattccttt tcaagcttta gccatgaaac gttacaatgc cattcgggga
    29881 accgatgttc cttattttcc aaatttgaaa gaattaaaac ataatatcat ggctctagat
    29941 gaggcagagt tacaagccta tcataagaca tcgtttgaaa gtgtgttgtc attaaatgga
    30001 gtcatcatcc cttccttgta tgtgatttta ttttttgtct atctattcac tggtcaagtg
    30061 gagttaacag ccatcttagt tttggtgtta attcagctct atttactcgt taagagtgca
    30121 acaatgacca gacaatttta ccgttagcct aaaaggagac tgtttatgaa aggattcatt
    30181 aattatttaa aaatagcagt gctcattatt ctggctatgg ttttcaatgt ccttccgatg
    30241 attttattgc aaaagcaaca cgatattcct atggtgctta attggggaat tggtattttc
    30301 tacttggtta ttgttggaag tgtccttatt gtattatggc gtctttatca agctaagcaa
    30361 gacaccccta ttaaacagca aaaaatgaga ttggttgact ggggttattt agcattattt
    30421 tggttaatca tccgtgtgat agctattgca ggtacccttg ttaaccagct atggtctggt
    30481 caacaagtga gtgctaatga tgctgcaata cataccttag ctagacttat caaaggtggt
    30541 ttcccgcttt atactgccct atttgtactt gtgatagctt ttatcgctcc tattatggaa
    30601 gaactagtct ttagaggatt tcctatgatt gatctcttca aaggaaaatc acttaaggtg
    30661 gcaggtttag tgacctctct tgtttttgct ttaccacatg ccaccaatag tgttgaattt
    30721 atcatgtaca gctgtatggg catttttctc tttgttgcct atcaaagacg aggaaactta
    30781 aaagacgcta tcttgttaca tatttttaat aacttgattg aagtcatttt gttaatgtca
    30841 ataggcttag gagtcatata ataaaaagct tcggatggat gaactgcacc ccaaaagttg
    30901 gacaaaaaaa tctaacattt gggttggaat tgtcaagaaa aaatagacat aaagttaagc
    30961 taatgagtaa ctattttttt caaattcaac aggtgagaga taccctaaag ctgaatgcag
    31021 acgtttgggg ttatagtaag tctcactata tttaaaaatc tcaagcttag cttgctcaat
    31081 agttgcgaaa tgagcatcat tgacaagctc tctttttaat gttttataaa aggattccat
    31141 gagtgcatta tcgtatggat taccttttcg actcatcctg gacttacatt ttttccatct
    31201 aagtaggtcc tgaaatcgtg ctccggtata ttgagaaccc tgatctgtat ggacgattac
    31261 cccttcttta ggcttttctc tattataagc ttgattgaaa gcttctgtta ccaacttgtc
    31321 ttgcatacgt tggcccattg cccaaccaac aacctttcta ctataaacgt ctatcaagac
    31381 agacacataa agtattcctt cttgtgtagg aatgtaggtt aggtctccta gccataattt
    31441 attcttacca gtcgcttgga aacattgatt aacaagattg ggacgagttc atgaagaacg
    31501 tcgacggtta tagtatttat attgatatcg acttcctt
//
LOCUS       DAUVUY010000009        29938 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.009, whole genome shotgun sequence.
ACCESSION   DAUVUY010000009 DAUVUY010000000
VERSION     DAUVUY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29938
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.009"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..135
                     /locus_tag="VOX58_000427"
     CDS             <1..135
                     /locus_tag="VOX58_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115075552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HES5877690.1"
                     /translation="NQQVKGKGNKLPATGENATPFFNVAALTIISSVGLLSVSKKKED
                     "
     gene            complement(641..790)
                     /locus_tag="VOX58_000428"
                     /pseudo
     CDS             complement(641..790)
                     /locus_tag="VOX58_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            847..1140
                     /locus_tag="VOX58_000429"
     CDS             847..1140
                     /locus_tag="VOX58_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HES5877691.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNQSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            1137..1475
                     /locus_tag="VOX58_000430"
     CDS             1137..1475
                     /locus_tag="VOX58_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877692.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRLTT
                     KYENKSDNIKSQYLEVIDWAKANLKRRSWIDTIQYYDLEDKGFNIKIIGKLSDRDIER
                     LKDFLRAKEV"
     gene            1478..1834
                     /locus_tag="VOX58_000431"
     CDS             1478..1834
                     /locus_tag="VOX58_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877693.1"
                     /translation="MKKKLVGFIVLALSTIILVACSNDSLEGEYYWINDARNQHMATI
                     KGDKGYVESEGGYSIKIDSELKIIESKFGSEKYSYKDGKLTTNFTGVESDFYKKGSKA
                     CEEALKKYGYKEVGKE"
     gene            complement(1899..2321)
                     /locus_tag="VOX58_000432"
     CDS             complement(1899..2321)
                     /locus_tag="VOX58_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HES5877694.1"
                     /translation="MDLSTVIKKTEDLGITILFCNMPKTKGRYDSTLEKPCIYVDKSL
                     SDIEKINIILHEKMHFTKNDQSNCLSYISSYSHHIENQAEKDRILDFMNLINEEYPID
                     ESFNYINYMKNACVPEKYESYIKNLAKEFYLRNHRKES"
     gene            complement(2328..2666)
                     /locus_tag="VOX58_000433"
     CDS             complement(2328..2666)
                     /locus_tag="VOX58_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5877695.1"
                     /translation="MLPEKLKTLRTEAGLTQKELAKIIQTSQQNIAYWEKGSRNPKHK
                     SIEKLANVFNVSTDYLLGNTDLKNPNDLDIEKVKESLKKSLGYNGTSDIPEDELESMA
                     QAFIEHFKNQ"
     gene            3589..4023
                     /locus_tag="VOX58_000434"
     CDS             3589..4023
                     /locus_tag="VOX58_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5877696.1"
                     /translation="MIDVSKKLKQLRIDRGIAKSRLITYLSITLRTYDRYEDGSRTPD
                     LVTLMKLSDFYQCSLDELVKGNTNDTLVDRALIVSYRDLKDKGLNDKLAHGLVKQVYD
                     TYEFEKIPQISDANRIKFIYKEDLVTLLESIVSKIQEEKKCD"
     gene            4181..4621
                     /locus_tag="VOX58_000435"
     CDS             4181..4621
                     /locus_tag="VOX58_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HES5877697.1"
                     /translation="MEHRLSMHYFQVKKEIFGVLLLSPVILLIMWGYVSSNQDDGRLT
                     QKQYYETVVNQTVNLVFYKKSCPYCKVAKKEIATQAKDSQVITYYVDTSTGEGQKLVK
                     KYHVKYAPTIVSIRKGNIQSFLYARDRGNNIIVEKEKIKEVFSQ"
     gene            4745..5137
                     /locus_tag="VOX58_000436"
     CDS             4745..5137
                     /locus_tag="VOX58_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877698.1"
                     /translation="MLNNLFIEKKGIMMSRKGVQEYDVTNVSERSIKIIKKAMYDEGT
                     GFKPIHFYGIAICEGTREFYRPTYPFVRSEEDLDNLKDFINLYETDLLTFYTHGHNYD
                     FGCFIYGIGNDGKDKFRDRWFKEGVIFY"
     gene            5230..7470
                     /locus_tag="VOX58_000437"
     CDS             5230..7470
                     /locus_tag="VOX58_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HES5877699.1"
                     /translation="MKQTKVLTGIAVAALSTGTGLVQADDLSNTRPAKAAEVVTHTEV
                     TENQVADAKTKADEATKAVKDQEATVKAIEAEKSQAQQSLVEATTAVNDTEKLASEAT
                     PDGLTKAQEETEASQSAVTDAQAQLEAAQEAESKAQTAVDYQKETVAKASQAVNQAKA
                     ALTTSNQAADVSAAEEKVLEAQEAVTKAETQVSQAQEADAKHESDVRHAKEELDLKSQ
                     KLTEKQTTLEQVMAAIEAERLTKPVENGTYFNQRDNEWQKAYGGKTFAATGCVPSALA
                     MVFSELAKREVTPTEIADYLWHNTDEFNKHYGGTSGKGLVAATNHYGFASTHLDSQST
                     IIAALQAGHHVLAAVQNNKFSPWGSQYSHEIVLRGYSNGNTYVYDPYNRANIGWYPIA
                     NLWNEQSRDAIDTAEVGVPFFKITTQKMAQLEMAKAVANEGLDEAKQAVKQAEANVTA
                     LEQVISQTSQAKEQLAAAKANLVTAKDMLDKAKQTAKLTEQDLETKTANLKQAQEHLT
                     LKQDDLAKAQTELAAQEKSLEQLTQVLSLAQENLGRATKAVEQTKADLTQKQAYVTNL
                     KQVTKLRQESQAKVNQAKAVLSEASARLEQELETLKTLQAKEANLTAQYQAIFKAYQA
                     VLAVKHHSQLAESKAKLEAKGEEAVPVVNEVGHVTDYVSGPTQLADAVAVRTEETSSK
                     TKNTSPVSVKLMAKQGHQKALETIVTSQSSLPQTGEQVVSILSLVGLNFLGLSSFLGL
                     RKQEEK"
     gene            7640..11230
                     /locus_tag="VOX58_000438"
     CDS             7640..11230
                     /locus_tag="VOX58_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HES5877700.1"
                     /translation="MKQMETKGYGYFRKTKAYGLVCGITLAGALTLGTTSVSADDVTT
                     LNPATNLTTLQTPPTADQTQLAHQAGQQSGELVSEVSNTEWDNAVTTAQKAGVTVKQS
                     EKVTHDSLSSAQADLEKQTQAVTEATTKQEANTTAINQAVSENKAIDQANRDEKARVD
                     ALNTKGEADTKAKNETGQAQVDAQNKQAQAAADAMNAKLKADYEAKLTEIKQIESENE
                     AIRQRNQQASQATHQANQAAQAAYQEKLAEIERINAENAAIRDRNTKARQEAERQNQQ
                     LKTTYEAQLSAYQQALQAKKEAENKAIDQVVFGIDAKANGVDNAEYGNSIMTVTTQPD
                     GSFVFKHDMIDGVKTIGHGTLTGKINHHYEANKDGSITAYIDSVTFDKYEYQNVAKND
                     AVDKNIAFRILSATGQELFVKAHDGDKTFSETLNKTVSLKLTYQLKPHEAVKDIKVFQ
                     LHDDWVHDTHGSALVSYVNNNDAVPHIEVPEKPVEPDMVTPKVEQEKPVPEAPQKPTD
                     GVPNLEKEKPVPPTPVKPEAVKPVLEQEKPVPEAPQKPTDDVPNLEKEKPVPPTPVKP
                     EAVKPVLEQEKPVPEAPQKPTDDVPNLEKEKPVPPTPVEPEAIKPDLQSFTPEVYDPI
                     KPVVKPHVTVPEKVVYEVMVHPVQVKQTPTNVKSVTNSDQVNIDGQLVPKGSTVTWEL
                     VNTSLKAGRQDITSYELTDPLPDGFELDVTATQTLSPEWVITTDEAGKVSLKASQSLL
                     AHFNAKRDQDVEVPKVSLVGRLLNDAGTYHNTFKTVVTTPTGSYTVISNTPVIYTPGN
                     DPKTPRNPGGDNPTPHDNLIQPTKTIVDDKGQSIDGKSVLPNSTLTYVAKQDFDQYKG
                     MTAAKESVMKGFIYVDDYKDEAIDGHSLVVNSIKAANGDDVTNLLEMRHVLSQDTLDD
                     KLKALIKASGISPVGEFYMWVAKDPAAFYKAYVQKGLDITYNLSFKLKQDFKKGDITN
                     QTYQIDFGNGYYGNIVVNHLSELTVHKDVFDKEGGQSINAGTVKVGDEVTYRLEGWVV
                     PTNRGYDLTEYKFVDQLQHTHDLYQKDKVLATVDITLSDGSVITKGTDLAKYTETVYN
                     KETGHYELAFKQDFLAKVVRSSEFGADAFVVVKRIKAGDVANEYTLYVNGNPVKSNKV
                     TTHTPEQPQPVTPKAPALPSTGEQGVSILTALGAALLSLLGYVGLKKRQQ"
     gene            11492..11797
                     /locus_tag="VOX58_000439"
     CDS             11492..11797
                     /locus_tag="VOX58_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HES5877701.1"
                     /translation="MEKSTLKLKPDFIGKAWYEKLGNAPRRIDGEAGQFQRHIVSNEK
                     HGVFHVITPALAQVFKTDDVVEIDGEAIFFEDRALNGNSVAPALNVRATGLKLAGGK"
     gene            11800..12255
                     /locus_tag="VOX58_000440"
     CDS             11800..12255
                     /locus_tag="VOX58_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HES5877702.1"
                     /translation="MAKVGLNFGEKIQIVDKSYRVLNDGLVDLKEIFGDLSFRSFEET
                     DLIYEDDMTQPRRQDGSYPQIPTGESRGLVVGIFSPVQQQTEFVTLVDMSLAEFEALQ
                     LKYREPVELEGIIVTYSSVGSNQFKLFASKIKRSVSQKVANEGNHKQEK"
     gene            12275..13984
                     /locus_tag="VOX58_000441"
     CDS             12275..13984
                     /locus_tag="VOX58_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HES5877703.1"
                     /translation="MRLLPEYRGIKVKPYMRYMSYYLFSFLFILFLIPVGVYSYYHLD
                     LLKMMDKMTMIILGISLFLSLFVSWYLTWFLQEANPLFNKLDRLKRLAKFLFENGFVY
                     EKRKKVNKKQKVSFPKVYLKQGKFDLSVSFEMAGNKFQKRFKDIGGELEDTFFMDFME
                     KTDDPRFKIYKLAYSAFLARITVNDVVWEKDKGIKLMNGYYWDFINDPHLLVAGGTGG
                     GKTVLLRSILKCLATIGVADICDPKRADFVTMADLPAFRGRIVFEKVDIIAKFENAIT
                     IMYARYDFIRKEMKRLGHKDMGKFYDYGLEPYFFICDEYNALMSSLSYQERDIVENAF
                     TQYILLGRQVGCNAIIAMQKPSADDLPTKIRSNMMHHISVGRLDDGGYMMLFGDENRN
                     KEFRYIKFLSGHRVYGRGYSAVFGEVAREFYAPLLTKKFSFYDEFAKISRHDNPFDPL
                     ENTLVSKDIISDESLRKFVEKTSDNELPKGSIGFDFEEEMEEINHMSIGDLAKKTGFS
                     FGKIKNLIDMIEKDGYMTFNREDERVVLTDSDAVILNSLLTQKDKFDGSWKELLLNYF
                     KGE"
     gene            14296..15537
                     /locus_tag="VOX58_000442"
     CDS             14296..15537
                     /locus_tag="VOX58_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HES5877704.1"
                     /translation="MNPMYLKKFRKKSGLKQKEFAKCIGISQAMLSQYESGKKKLSLE
                     KFQEMKTHFGYLTDNDSSRLQVTIDYVRITLKSVRDLEFFCRNFLHCRFKEFSSVESK
                     LMNYNHIWKRGDIWIFDYADKHDTGNYQITLQLSGQGCRQLELLMEQENFAWLDWLIY
                     LRRSYRDEMNVTRFDLAIDELYLGKDRENEHFLLSDMIAKYYHHELAFESLRTWNYIG
                     GGALNFNDMEEIEQNRQGISLYFGSRQSEMYFNFYEKRYELAKLEGISVEESLEIFEI
                     WNRYEIRLSQSKANAVVDEFISGIPLGEIARGLINSKIQVYDGKNKYGAFMADQKWQM
                     MFGGVEPLTFVTRPESYNIERTIRWLIHQVSDSLAMVSEYDSMIDGDNLQMIINSGEV
                     NERGEKILETIRYNLGLDKGA"
     gene            15538..15915
                     /locus_tag="VOX58_000443"
     CDS             15538..15915
                     /locus_tag="VOX58_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877705.1"
                     /translation="MFLENEFNYFISTRNNLELVIDSLVLMIPDREFYYPEIQTGELR
                     DYQIDIYDLIKIGYVGVYEIQKDYEDKLRELADFKRKLLKFGLLMQPLEKQKEIVIRL
                     AAKYHLEKRILMKKEMFRDEEVD"
     gene            15966..16171
                     /locus_tag="VOX58_000444"
                     /pseudo
     CDS             15966..16171
                     /locus_tag="VOX58_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057932.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            16304..16576
                     /locus_tag="VOX58_000445"
     CDS             16304..16576
                     /locus_tag="VOX58_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877706.1"
                     /translation="MSNTRSEADKKLLVVTQELSELLISHQYDQSWEKAGELNSLLKK
                     REELTLPGYMVDMIQQHLKSYYYQNNMINKAHKSMSAIGHKLQEFH"
     gene            16629..17110
                     /locus_tag="VOX58_000446"
                     /pseudo
     CDS             16629..17110
                     /locus_tag="VOX58_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
     gene            17118..17597
                     /locus_tag="VOX58_000447"
     CDS             17118..17597
                     /locus_tag="VOX58_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877707.1"
                     /translation="MIAIIRTHEKGRSQFLCELDIMQFTENQVRDHMIERGIKDDAFF
                     ICGFSDWNVDRIMSLSEVYLLKRCIVGLYDGDDYIVQYLLKKGLSVPAIVTKFYVFLS
                     KDEKEAMGYVLKNVSFDSLIDFWQRSVTWANALNAYIQSGILLNTSKGFYVLKTEGG"
     gene            17598..18548
                     /locus_tag="VOX58_000448"
     CDS             17598..18548
                     /locus_tag="VOX58_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HES5877708.1"
                     /translation="MDYLVKIKKFLLRYTKKEKGGKQPKPKEVKQKTANIIVYGILGL
                     LFIVGFFGSLRAIGLSNQVNSLKATVLSSKQQNGKDTKEILDIPRVQYYMNNFVYTYI
                     NYSDDTATKRKEELDNYYSFSTANLVDDVKKERKLQTQRLVSVEQEEDYHIALMRIGY
                     EVDSKSYQMTLAIPFRMANGLLAIVSPPYTLAEDLFQGKSKSFERKTVDQVKSLPKQE
                     MLSIQKFLPVFFDKYALSNEIDLKLLMKHPELMGGNYRVKSLDANNARYYQDKSNQVV
                     QLMVTFEDMVTGGTRSENFTLYLIKSDNGWYVDKLYHYFK"
     gene            18567..18836
                     /locus_tag="VOX58_000449"
     CDS             18567..18836
                     /locus_tag="VOX58_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877709.1"
                     /translation="MKVSKVAQVVLTGGGPNVSGLKQFFQGDGIYLISVICAFLVIKN
                     WKNADWLKVGSVLSIYAIVVSLFKGQEILAFLGGMLRWFGIETGL"
     gene            18839..19249
                     /locus_tag="VOX58_000450"
     CDS             18839..19249
                     /locus_tag="VOX58_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HES5877710.1"
                     /translation="MEEEKYFDYSRGLNAPYWIQEIKTSKGKLLWYFSTPMQLSFFIV
                     FFLILVLMLTVFSAPMQLLNQLTHSISLLLYWFVPYRLSKFYTEYEPQGKKMHTYLWD
                     YLVYLIDYGFNKKAIYQGERVEVLEEIVFEKTNI"
     gene            19270..21771
                     /locus_tag="VOX58_000451"
     CDS             19270..21771
                     /locus_tag="VOX58_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HES5877711.1"
                     /translation="MVKKLEYPINSIHSNLALRKDKVVIAYYRIPNTPITITDSDKKD
                     SHKDKVAQVIKKLAKYKHFDISLVPKDYLLEEKMSDFIGDLADDVKDLGIDTLSYTVD
                     TLTAEIEIPYQFDWVIGVNLGRDTMSATLKELAVKQLDLIADNVASLLGYTVELDKEW
                     YKEYQDEELLLYGLLSSLKAKRLSEEDLFYYQRMQYLRYIPHAKNEVIANRTLLNVTD
                     TLVKPLSGGFLKLESPYGSSFITILPVGKFNTIFNGFHLGELVQRMSFPVELRFKAEF
                     INKTKLSGTMGRSNTRYDQIMKEAYSTNTVQQDDILMGSYSLKDLMKKVGNKEEIIEY
                     GCFLVVSGSSIKQLRQRRHAVLSYFADMQVNVHEASHDTPYLFQTLLYGQNLQKTTRK
                     WNHLVTSRGFSEMMLFTNTQSGNRIGWYIGRVDNRLSAWDNIGEAIMGSKNLVLFNAT
                     VANKEDVAGKVTKNPHIIITGATGQGKSYLAQMIFLHTAQQNVRVLYIDPKRELRKHY
                     LQVVSDPEYAKQFPLRKHQIEQINFVTLDSAMKENHGVLDPIVILDKEDASSTAKNIL
                     LYLLRNATEVKIVQTTALTEAISEVISRRENGEVVGFNQVIETLCQSDNQDIVDVGHY
                     FKAIIKNSILELAFSDGNVKGLSYEERVTVLEVADLSLPKDGSDHISDHESNSIALMF
                     ALGAFCKHFGERTNEETVEIFDEAWILMQSSEGKAVIKSMRRVGRSKYNVLMLVSQSV
                     HDAENDDDTTGFGTIFAFYEKSEREAILTHVGLEVNEKNLEWIDNMISGQCLYYDVYG
                     NLNMMSVHNIHPDIDPLLKPMKQSVSSHLENKYAS"
     gene            21786..22958
                     /locus_tag="VOX58_000452"
                     /pseudo
     CDS             21786..22958
                     /locus_tag="VOX58_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23532..24101
                     /locus_tag="VOX58_000453"
     CDS             23532..24101
                     /locus_tag="VOX58_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877712.1"
                     /translation="MGKHSPMNFSKPSQMTESIDHDVHYDLPGVNEEMESPFQSESDY
                     QSQNSSIIVETPEMDDMIYQSSQKKNISIGNNYSSDKKSLENSFDSPIIPERSEPETD
                     AKERFNPQAEYQRLKAKRQKPLGNRKKSKLEAELGQFTDDASYYKAHGGTAFKRGFNA
                     SKSKELRLKQNLERKATILAELDKLRGHV"
     gene            complement(24269..24412)
                     /locus_tag="VOX58_000454"
     CDS             complement(24269..24412)
                     /locus_tag="VOX58_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HES5877713.1"
                     /translation="MGNSSKSNRKEELDLSTFEVLTLMFIAGNFVIALVKLVLELVKS
                     TKK"
     gene            24553..25905
                     /locus_tag="VOX58_000455"
     CDS             24553..25905
                     /locus_tag="VOX58_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HES5877714.1"
                     /translation="MSIPFFIVVIVTIIFSSSQGDCQVTPDLSTQFVTSSDSASQTDW
                     TKKGSIANVTAGKVFHAWVSKGLSGASAAGIVGWVNSEGGFAMIGRAEGHYGNDLRTN
                     SIAFGVKPIGLSYYTTEAGGGIYQFTPYTKYAPLGDPKWENADAMNEFVAKAILAGDW
                     NASMDLTGGNHTFKQMAQMTDPKQATLVWQAYERGSTAHINPFQKQADAQKAYELFGG
                     VKYSYNEIVFHKSFDIFGPLKPGEGESKEVVLSLCESGSYSKGAFGKDRTGKVNYTSY
                     NAWRPEQLPADLKPYALDPRSVGLNYRQAVGWYAIASTGGQCTDLSASLMYALWSKNG
                     SHPTQKAGNGNMVVANWVRTFGGSSDKSPTSGAVFSSAGTSSAGHTGVVSHVFENGDI
                     LIVEQNYASYSGDDGGFGKYSWNYRYVTTAELKNEGYTFYNPAKLGYQIMKSVGTVGK
                     "
     gene            25905..26462
                     /locus_tag="VOX58_000456"
     CDS             25905..26462
                     /locus_tag="VOX58_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877715.1"
                     /translation="MKIRKVIIVMLAVALSLGFYRLYHRQKPSQLIEANQEILVKPIT
                     PNKSKIKVKRNVAKEAKATLEMPEERVNEKEAKQVKSAIVIPIDYLASISSQDDIKVT
                     YDNKLSITNPAMAASIKTMLLAGYHFDLDSLTVYQSNSDNVYQFTILLVTHQQDQLSL
                     VGNYVTGTGQFEFVSLHGTPKNVMF"
     gene            26487..27248
                     /locus_tag="VOX58_000457"
     CDS             26487..27248
                     /locus_tag="VOX58_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HES5877716.1"
                     /translation="MTKEKMMSFMTIASIALLGSGVVCANDVADPVAGTTDAVVVPVS
                     PQPTTPIEHTGTDTTNKIVDSTKPSEDKVADSTDQLIPPVKSDLETSEDKVTDPTETK
                     PSKEKTEEPVKSNDSEVIPEQPKSEEVKKAEENTGASTTETPKPVTKPVIETPIVTNT
                     GYTVLGTQDGNVWVQTETGNVLKPAIAIGAVKQKDGTVALKTRDGKLEVLPSTGETKT
                     ILAILGGLAILGAIWVGWKENIKKYLTFLKKKSKK"
     gene            27471..28013
                     /locus_tag="VOX58_000458"
     CDS             27471..28013
                     /locus_tag="VOX58_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877717.1"
                     /translation="MLNYKYITLNDSYELNVWLDFVLRDLKKEEKYSKFRFIIKKLSI
                     KNSRTTFQEIESVRKELMGLSELDLKHLRMIIENRETTGIGLEFKAFLQKIFNYFGVI
                     VVGLIPVILGYNSIERVVTSWLNAKTFKSSILLIVWIFVLYIYYVVFKEFFLHDSKKR
                     EVRKYLLFLINDILLDMEKR"
     gene            28186..28512
                     /locus_tag="VOX58_000459"
     CDS             28186..28512
                     /locus_tag="VOX58_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HES5877718.1"
                     /translation="MQQVFNVSVPVPDDVVIISKEEYLDLLSDNEQGKWWDIDNLQEL
                     LGLGRSKLINDILLNPDIKKEVDLSINPNGFIVYPRGKGSRYKILATRARKYFEENFG
                     SILLNN"
     gene            28562..29698
                     /locus_tag="VOX58_000460"
     CDS             28562..29698
                     /locus_tag="VOX58_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HES5877719.1"
                     /translation="MATYRQRGKKKLWDYRVYGKDGKLIASGSGFRTKKEASYEALKI
                     EQKKYYSDVINSNATLFEMWQIWYDLVVEPSELDILTKQKYQARGKIIEKYLSDIPAN
                     RIKHSKYQEFIKFYGKNVTLNLMTCLNSDIRKVIIFFKRDGVLIEDFTEGVKIFGKPY
                     RKSPEDKYLKSIEDYQKLLAYLKRIANYSDSIIPFFLFTMLQTGFRFGEAMAITWDDI
                     DFERNSIYTYRRYSSIKKDFTKPKTRTSIRRVPVSHDLKELLLELKFQQERMLRGLHI
                     KNTDQLIFYDYRFGMISNNAVNKFLASSLEKLEIESKMSSTGARHTYGSYLLANGVDI
                     WAVAKLMGHKDIKQLIETYGHLLLEVEEKENDQLLKILNNKGSF"
     gene            complement(29774..29844)
                     /locus_tag="VOX58_000461"
     tRNA            complement(29774..29844)
                     /locus_tag="VOX58_000461"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29810..29812),aa:Thr,seq:ggt)
BASE COUNT        10333 a         4209 c         6062 g         9334 t
ORIGIN      
        1 aatcagcaag tcaaaggtaa aggaaataaa ctaccagcaa caggtgagaa tgcaactcca
       61 ttctttaatg ttgcagcttt gacaattata tcatcagttg gtttattatc tgtttctaag
      121 aaaaaagagg attaatcttt tgacctaaaa tgtcactaaa cttttcacca tttattggtg
      181 tgaacacatt aataaagtta tgtatctctc tccaacaaac ttaattaaag tatttcaatt
      241 tttcgagttt aattcttgaa aaagcctatc gagattatta atttcgatag gcttttgatt
      301 ttgtgtaagc gtccaataac gaggtatctt ccattttctt ccctattccg taagcatcca
      361 atataccttg ttattggacg cttactataa aactgaagaa atgatcctat ttagttgaaa
      421 tttgtaaatg aagtgtatca ttagagccta agatgttaat cctaggctct cttttgttga
      481 tttaaaattt ttaggtgtaa tcaaatgtaa ggttagatgc aaaattttct aaaatgactg
      541 tttgtctttt caagtaatta aatgtattta aggggaattt ataaaaataa aaggagtgtg
      601 tcaataatgt tgtgtaaaca cactggcaaa gctgggattg ttaatcaaat aggctttcaa
      661 gtgtgtctgt acaatgacca aaacctttgt ggactcgttg ttcttgcttg aagttgtaat
      721 cattaaatag tgtgactaga cagtgttcta gggtctcttc actgggaaat acgattttct
      781 tttttgtttg cgttgtattg cagttagtga tacaatggtt atacaaaaat gatttgaggt
      841 gatattatgt caacagtagc agtaagagtt gatgatcaat taaaagatga tgctacagaa
      901 ctttttcaat ccttagggtt ggatatgagt acagcagtaa aaatgttttt gattcagtct
      961 gttaaaactc aaagtattcc ttttgaaata aaaaatcaat catctgtttc tgatgaagaa
     1021 tttcaaaatt tggtagaaac aaaattaaaa ggaatacgag ttaaggctag tgatccagaa
     1081 agtgtaaatg cgttttttgg agatgaagat ttttcagaat atgaggaata ttttaaatga
     1141 gtgatattga ggtatattct gtattagttt cacgtattga atattctgat ggtacaggtt
     1201 caaaggttcg tcctgctgta gtagtcaaat ttaatgatga agtaataaaa actttaagat
     1261 taactactaa atatgaaaat aagtctgaca atataaaaag tcagtattta gaagttattg
     1321 attgggcaaa agcaaattta aaaaggcgtt catggattga tacaattcag tactatgatt
     1381 tagaggacaa gggattcaat attaaaatta ttggtaagtt aagtgataga gatattgaaa
     1441 gattaaaaga ttttctcaga gcaaaggaag tttagttatg aaaaagaaat tagttggttt
     1501 tattgttcta gctttatcta caataatttt ggtagcatgt tctaatgatt cacttgaagg
     1561 tgaatattat tggattaatg atgctagaaa tcagcatatg gctacaatta aaggcgataa
     1621 gggttatgtt gaatcagaag gcggttattc cataaaaatt gattcagagt taaaaattat
     1681 tgaaagtaaa tttggttctg aaaaatatag ttataaggat ggaaaattga caacaaattt
     1741 tactggtgta gaaagtgatt tttataaaaa gggatcaaaa gcttgtgagg aagcattgaa
     1801 aaaatatggt tataaagaag taggaaaaga atagtatttt attatattct gttccatgaa
     1861 aattattttt cagaataaaa aggagagcta actaactact agctctcttt tctgtgattt
     1921 cttagataaa attctttagc gagatttttg atataacttt catacttttc aggtacacaa
     1981 gcatttttca tatagttgat atagttaaaa gattcatcaa tcggatattc ttcgttaatt
     2041 agattcataa aatctaatat gcggtctttt tcagcttgat tttcgatatg atgtgaatat
     2101 gaagaaatat aagataagca gttactctga tcattttttg taaagtgcat tttttcatga
     2161 agaatgatgt taattttttc tatatctgaa agacttttgt caacataaat gcaaggcttt
     2221 tcaagtgtgc tgtcatatct tcctttggtt tttggcatat tacaaaataa gatagtaatt
     2281 cctaaatctt ccgttttttt aattacagtt gataaatcca ttattaatta ttgattttta
     2341 aagtgttcaa taaaagcttg tgccatactt tctagctcat cttcaggaat atcagaagtt
     2401 ccattgtaac caagagattt ttttaggctc tcttttactt tttcaatgtc taaatcatta
     2461 ggattcttta aatcagtatt tcccaataaa taatctgtag aaacgttaaa aacgttagct
     2521 aatttttcta ttgatttatg cttgggattt ctactaccct tctcccaata tgcaatattt
     2581 tgttgactag tttgaataat tttagctaat tctttttgag taagtccagc ttctgttcgt
     2641 aatgttttta atttttctgg aagcatagtt gtacctttca aaattatatt tttttgaaag
     2701 aaatctattg acacataaca acaaatgttg tattatatat ttaacaacaa aagttgttag
     2761 actctttcaa aaaaagtgtt gaagtattta tcaaaataaa aaaataaaac actaatttga
     2821 ggaatagagt taaattttag aacattgata attaaataaa aagtgcgtca gacaacacag
     2881 ctaagctaag ttgaataaca cacataataa aacatagaca cttttattat agcgtgaaat
     2941 tcttcagaag taaagcaagt gtagtttact ataccaagtt gtctgataac ttttgaagtt
     3001 tgaattttgc ttgtttaagc aaaaaaagtt agatagttat tcctacctag cttagtttta
     3061 ctataaaaat tgatgcaaaa tagttgtggc catgattata gtaattactg tgtgttcaac
     3121 taaacaagta aagttcagac ctcaaaagca cactaaggtt tatgttagtt agcctgtgtt
     3181 gagcaaaaaa ctaaaactgc tactctgcaa ggggtgttga caatgattac tgaaaacgtg
     3241 gtgaggcgga ttaggtatgg tattgtgggc ggtgtgttag actttgtcta gctaaaggat
     3301 aaggtggact tattcgactg agtagctcat gttgagaaac atagtgaaag gagtgtgcga
     3361 agatacgagc atgcaattta aattgaaata tagtgagaat atttttttct atgtcgactt
     3421 ctttcataag tcgacttttt tgttaaaaaa taaaaataga aatatagttt aggtattatg
     3481 gggtatcgtc ctttctctaa gctatatttt tttatttttg gaaagggtat taagcatagt
     3541 atctttttta aaggtaaaaa tttagaatga ttgaaaggga gctgaataat gattgatgta
     3601 tcaaaaaaac taaaacaact aagaattgat agagggattg ctaaatcgcg gttgatcact
     3661 tatttatcga taactttaag aacttatgac agatatgaag atggtagtcg tactcctgat
     3721 ttagtaacgt taatgaaatt atcggatttt tatcaatgct cgcttgatga attagttaaa
     3781 gggaacacaa atgatacgct tgttgatagg gcattgattg tttcgtatag agatcttaaa
     3841 gataagggtt taaatgataa attggctcat ggtctagtga agcaggtcta tgatacttat
     3901 gaatttgaga agattcctca aatatctgat gctaatagaa ttaagtttat ctataaagaa
     3961 gatttggtta ctttacttga aagtatcgtg tcaaaaatac aagaggagaa gaaatgtgac
     4021 tagctaaaag attttggagg taatgaatgt tagtttatga atttattaaa caaaagattt
     4081 tgtgtcatca gctaaccagg ttgttacgaa taggtactgt aatgataact ggactctcct
     4141 atagtttaga taactggaaa aagtggaggg ggattgaaat atggagcatc gattgagtat
     4201 gcactatttt caagtgaaaa aagaaatttt tggggtatta ttattgagtc ccgttatttt
     4261 attgattatg tggggctatg tttctagcaa tcaagatgat ggtcgactaa cgcaaaagca
     4321 atactatgaa acagtggtca accaaacggt taatttagtg ttttataaaa agtcatgtcc
     4381 ctattgcaaa gtagctaaaa aagaaattgc tactcaagct aaggatagtc aagttattac
     4441 ttattatgtt gatactagta cgggagaagg acaaaaattg gttaaaaaat accatgttaa
     4501 gtacgcacct acaatagtat caattagaaa aggaaatatt caatcatttt tgtatgctcg
     4561 agatagaggc aacaacatta tagtagaaaa agaaaagata aaagaagtat ttagtcagta
     4621 agctatgact ttatgtgtca tagctttttt atatttgttg aaaacaaggg taaaatacag
     4681 taaatctcca ttcttcattc tccattttta aaccgaaaag attttactct tgttttgagc
     4741 aagtatgctt aataatttat ttatagaaaa gaaaggtatt atgatgtcta ggaaaggagt
     4801 acaagaatac gatgtaacaa atgtatctga aagaagtata aagataatta agaaagctat
     4861 gtatgatgaa ggaacaggtt ttaagccaat tcatttttat ggaatagcta tctgcgaagg
     4921 gactagagaa ttttatagac ctacttatcc atttgttaga agtgaagaag atttagataa
     4981 cttaaaagat tttattaact tatatgagac agatttgtta actttttata cacatggtca
     5041 taattatgac tttggctgtt ttatttatgg tataggaaat gacggaaaag ataagttccg
     5101 tgatagatgg tttaaagaag gagtcatttt ctattgaatt ttaaaataat taaaaaaaag
     5161 atgacccaag gtaacattaa agtagtataa taatcttaat aaaggaagtt ataggaaggg
     5221 caactactta tgaaacaaac aaaagtatta acaggaatcg cagtcgcagc tttatctaca
     5281 ggtacaggtc ttgtgcaggc agatgattta tcaaacacaa gaccagctaa agcagctgaa
     5341 gttgtgacac atacggaagt gacagaaaat caagtggcgg acgctaaaac aaaagctgat
     5401 gaggcaacga aagccgttaa ggatcaggaa gctacggtta aggctattga ggcagaaaaa
     5461 tctcaagcac aacaatcttt agttgaggcg acaacagcag ttaatgatac cgaaaagtta
     5521 gctagtgaag cgacaccaga tggtctaaca aaagcccaag aagagactga ggctagtcaa
     5581 agcgctgtta ctgatgcaca agcacaactt gaagcagctc aagaggctga atcaaaagcc
     5641 caaacagcag ttgactatca gaaagaaaca gtcgccaaag cgtctcaagc tgttaatcaa
     5701 gccaaagcag ctctcacgac aagtaatcaa gccgctgatg ttagtgcagc agaagaaaaa
     5761 gtgttagagg ctcaagaagc ggttacgaaa gcagaaacac aagttagtca ggctcaagaa
     5821 gcagatgcta aacatgagtc tgatgtgaga catgccaaag aagagcttga ccttaagtct
     5881 caaaaattaa cagaaaaaca aacaactcta gaacaagtca tggctgcgat tgaagcagag
     5941 cgtctgacta agccagttga aaatggcact tactttaacc aaagagataa cgagtggcaa
     6001 aaagcctatg gtgggaagac ctttgcggcg acaggatgtg tgcctagtgc tttggctatg
     6061 gttttttcag agctggctaa aagagaggtg acaccgactg agattgctga ttacttatgg
     6121 cataacacag atgagtttaa taaacattat ggtggtacaa gtggtaaagg acttgtagct
     6181 gctactaatc attatggctt tgcttcaaca catttagata gtcaatcaac tattattgca
     6241 gctttacaag caggtcatca tgttttggct gcagtacaga ataataaatt tagtccttgg
     6301 ggatcacaat atagtcatga aattgtcttg cgtggttact caaatggcaa tacttatgtt
     6361 tacgatcctt ataatcgagc taacattggt tggtacccta ttgctaattt atggaatgaa
     6421 caaagtcgtg atgctattga tacagcagag gttggagtac ctttctttaa aataacaact
     6481 caaaaaatgg cacaactcga aatggcaaaa gcagtagcta atgaaggctt agatgaagct
     6541 aagcaagctg ttaaacaagc ggaagcaaat gtgacagcct tagaacaagt tatttctcaa
     6601 acctcacaag ccaaagaaca attagctgca gctaaggcta acttagtcac agccaaagat
     6661 atgctggata aggctaaaca aacggctaaa ttaacagaac aagacttaga aacgaaaaca
     6721 gctaatctta agcaagctca agaacatcta acccttaaac aagatgacct agctaaagct
     6781 caaacggagc tagcagctca agagaaaagc ttggaacagt taactcaagt gctttcatta
     6841 gctcaagaaa atcttggccg tgctacaaaa gcagttgaac agactaaggc tgatcttacc
     6901 caaaaacagg cttatgtgac gaatcttaag caagtgacaa aactgagaca agaaagtcaa
     6961 gccaaagtaa accaagctaa ggcggttctc agtgaagcaa gtgctcgttt agaacaagaa
     7021 cttgagacct taaaaacctt acaagccaaa gaggctaacc taacagcaca gtatcaagct
     7081 atctttaaag cctatcaagc tgttttagct gtgaaacacc actcacaatt ggctgaaagc
     7141 aaagctaagc ttgaagctaa gggagaagaa gctgttcctg tggtgaatga agtgggacac
     7201 gtaacggatt atgtgtctgg gccaacacag ttggctgatg ctgtagcagt gagaacagaa
     7261 gaaacatcat caaaaacaaa gaacacttct cctgttagtg tgaaattgat ggccaagcaa
     7321 ggtcatcaaa aggctcttga aactatcgtt acttcacaaa gtagccttcc tcagacaggt
     7381 gagcaagttg tctctatttt gagcttagta ggtttgaatt ttttaggctt aagtagtttt
     7441 ttggggctta ggaagcaaga ggaaaagtaa taaaatatag aacaagaggt cattctttag
     7501 agaatggctt tttgattgga gttatgatga aaatagtttt taattggaag gcaagattac
     7561 cttccttttc tttttagtaa aacatataag acctgggatc gtcacaaaaa gcaacctaga
     7621 agagagatga gaaaataaca tgaaacaaat ggaaactaag ggttatggtt attttagaaa
     7681 aacaaaggcc tatggtcttg tttgtggtat tactttagcg ggagcgttga cgttgggtac
     7741 aacaagtgtt tctgcggatg acgtaactac tttaaaccca gcgactaact taacaacgct
     7801 acaaacacct ccaacagctg atcaaacaca gctagctcat caagctggcc agcagtcagg
     7861 ggagttggta tcagaggtga gcaataccga atgggataat gctgtcacaa cagctcaaaa
     7921 agcaggtgtt actgtcaaac agtctgagaa ggtcacacat gatagcttat catctgcgca
     7981 agccgattta gagaaacaaa ctcaagctgt cacagaagcg acaactaaac aagaagccaa
     8041 cacaacagct attaatcaag cagtttcgga aaataaagcg attgatcaag ctaatcgtga
     8101 tgaaaaagct agagttgacg ctcttaatac taaaggggaa gctgatacca aagctaaaaa
     8161 tgaaactggt caagcccaag tagatgcaca aaataaacaa gcccaagcag cagctgatgc
     8221 catgaatgcc aaattaaagg cagactatga ggccaagtta acagaaatca agcagattga
     8281 atcagaaaat gaagctatcc gtcagcgcaa ccaacaagct agtcaagcga ctcatcaagc
     8341 caatcaagca gcccaagctg cctatcaaga aaagctagca gagattgagc gtattaacgc
     8401 cgagaatgcc gccattcgtg atcgcaacac taaagctcga caagaagcag aacgtcaaaa
     8461 ccaacagtta aaaactactt atgaagctca attatcagcc taccaacaag cactacaagc
     8521 taaaaaagaa gctgaaaaca aagctatcga tcaggttgtc tttggaattg atgcaaaagc
     8581 caacggtgtt gataatgcag aatatggtaa ttcaatcatg actgttacaa cgcaaccaga
     8641 tggttcgttt gtttttaagc atgatatgat tgatggtgtc aaaacaattg gtcatgggac
     8701 tttaacgggt aaaattaatc atcattatga ggctaataaa gatggtagca ttacagctta
     8761 tattgatagt gtaacatttg ataagtatga atatcaaaat gtagctaaaa atgacgcagt
     8821 tgataaaaat attgcttttc gtattttaag tgccacaggt caagaattat ttgttaaagc
     8881 ccatgatggt gataaaactt ttagtgaaac tttaaataag acagtgtctc ttaagttaac
     8941 ttaccaacta aaaccgcatg aagctgtaaa agatatcaaa gtatttcaac tgcatgatga
     9001 ctgggtacac gacacacatg gttctgcttt agtttcttat gtgaacaaca atgatgctgt
     9061 gccacatatt gaagtaccag aaaagccagt ggagcctgat atggttacac caaaagttga
     9121 gcaagaaaaa ccagtcccag aggctcctca aaaaccaaca gatggtgttc caaacttaga
     9181 gaaggaaaag ccggttcctc caaccccagt taaaccagag gcagttaaac ctgttttaga
     9241 acaagaaaaa ccagtaccag aggctcctca aaagccaaca gatgatgttc caaacttaga
     9301 gaaggaaaag ccggtaccac caactccagt taaaccagag gcagttaaac ctgttttaga
     9361 acaagaaaaa ccagtcccag aagctcctca aaagccaaca gatgatgttc caaacttaga
     9421 gaaggaaaaa ccagtaccac caaccccagt tgaacctgaa gcaattaagc cagatctcca
     9481 aagctttacg ccagaggttt atgacccgat taaaccagtt gtgaaacccc atgtcaccgt
     9541 tcctgaaaag gtcgtctatg aggttatggt tcatcctgtt caagtgaaac aaacgccaac
     9601 aaatgttaag tctgttacta atagtgatca ggttaatatc gatggacaac tggtaccaaa
     9661 aggatcaacg gtaacgtggg agcttgttaa tacttctctc aaagctggtc gtcaggacat
     9721 cacaagctat gaactaactg atccactacc agatggtttt gagttagatg ttaccgccac
     9781 acaaaccctt tctcctgaat gggtgataac aacggatgaa gcaggcaagg ttagtcttaa
     9841 ggcttctcaa tcccttttgg ctcacttcaa tgctaaacgt gatcaagatg tagaagtccc
     9901 taaggtatca ctggttggtc gtttactgaa tgatgcgggg acttatcaca atacctttaa
     9961 gacggtggtc actactccaa ctggtagcta cacggtaatc tctaacacac cagtgatcta
    10021 tacaccaggg aacgatccga aaacacctcg taacccagga ggagataatc caacaccgca
    10081 tgataatctg attcagccaa ctaaaaccat tgtggacgac aagggtcagt ccattgatgg
    10141 gaagtctgtc ttgccaaaca gcacgctgac ttacgtggct aagcaagatt ttgatcagta
    10201 caaagggatg acagctgcca aagaaagtgt catgaaaggc tttatctatg tcgatgatta
    10261 caaggatgaa gcgatagatg gtcattcact cgttgtgaat tccattaagg ccgctaacgg
    10321 agatgatgta accaatctct tagagatgcg ccatgtgtta tctcaagata cgcttgacga
    10381 taagctaaaa gcactcatca aggcatcagg catttctcca gtcggtgaat tttacatgtg
    10441 ggtagctaaa gacccagcag ccttttacaa agcttatgtt caaaaagggt tagacattac
    10501 ctacaacttg tcctttaaac ttaaacagga ctttaagaaa ggtgacatta ctaaccaaac
    10561 ctaccaaatt gattttggaa acggatacta cggtaatatc gtggtaaacc atctgtctga
    10621 actaactgtt cataaggatg ttttcgataa agaaggtggt caatcaatca atgctggcac
    10681 tgttaaagtt ggtgatgagg tcacctatcg cttggaaggt tgggttgttc cgactaaccg
    10741 tggctatgac ttgacagaat acaaatttgt ggatcagctt cagcatacgc atgacctcta
    10801 ccagaaagac aaggtccttg cgacggttga tattacttta tcagatggta gtgttatcac
    10861 taaaggtact gacttagcga aatatactga gacagtttac aataaggaaa caggtcacta
    10921 tgaattagcg ttcaagcaag acttcttagc taaggtcgtt cgttctagcg aatttggtgc
    10981 agatgctttt gtggttgtta aacgcatcaa agcaggtgat gtggcaaatg agtatacctt
    11041 gtatgtcaac ggtaatcctg tgaagtctaa taaagtcacc actcatacgc cagaacaacc
    11101 tcaacctgta actcctaaag caccagcttt accatccaca ggagagcaag gagtatctat
    11161 cttaacggcg cttggggccg ctttactctc actcttaggt tatgtagggc ttaaaaagcg
    11221 tcaacagtaa gttattaatg gataagccat caaagtagat tactttgggt ctatccattc
    11281 tctctagcaa gtaactatta gggggatctc ctttcccttc aaaaaactag ccgaaaaagt
    11341 ttactattgg ttacttgtta gggtgagtgg atagatcttg gtttatcact agaatagtga
    11401 ccgaaaagtc aaaaaactaa gtggtaaggc taggaaataa ttgataagac aatcctagcc
    11461 tttttatttt aaaaatgaaa agaggtaaat tatggagaaa tcaacattaa agttaaaacc
    11521 agattttatt ggtaaagcat ggtatgaaaa attaggaaat gctccccgtc ggattgatgg
    11581 cgaagcaggg cagtttcagc gtcatattgt gtcgaatgaa aagcatgggg tttttcatgt
    11641 gattacaccg gctttagccc aagtatttaa aacagatgat gtagttgaaa ttgatggtga
    11701 agctatcttt tttgaagata gagctttgaa tggtaatagt gtagcacctg cacttaatgt
    11761 acgtgcaaca ggattgaaat tagctggagg taaatagtaa tggcaaaagt aggtttaaat
    11821 tttggagaga aaattcagat tgtagataaa agttatcgcg tattaaatga tggtttagtt
    11881 gacttgaaag aaatttttgg tgacttatca tttcgatcct ttgaagaaac agatttaatt
    11941 tatgaagatg atatgactca accaagacgt caagatggtt cttacccaca aattcctaca
    12001 ggcgaatcac gtggacttgt tgttggtatt ttctcaccag tgcagcaaca aacagaattt
    12061 gttactttgg ttgatatgag ccttgcggag tttgaagcgt tgcagttgaa atatcgtgag
    12121 ccagttgaat tagaaggtat tatcgtgact tattcttctg taggaagtaa tcagtttaag
    12181 ttatttgctt ccaaaattaa acgctctgtt agccaaaaag tagctaatga aggtaatcat
    12241 aagcaagaaa aataaataat aagggagagg taagatgcgt ttactgcctg aatatagagg
    12301 gattaaggta aaaccatata tgaggtatat gagttattac cttttttctt ttctatttat
    12361 tttgtttttg atacctgtag gggtttatag ttactaccat ttagatttgt taaaaatgat
    12421 ggataaaatg actatgataa ttttagggat aagtttgttt ttatcgcttt ttgtttcttg
    12481 gtatttgact tggtttttac aagaagctaa tccattattt aataagttag atcgtttaaa
    12541 gcgtttggct aaatttttat ttgaaaatgg ttttgtttat gaaaaacgta aaaaagttaa
    12601 taagaagcag aaagttagtt ttccaaaagt ttatttaaaa caaggtaagt ttgatttaag
    12661 tgtctccttt gaaatggcag gaaacaaatt tcaaaagcgt ttcaaagata ttggaggtga
    12721 acttgaagat acttttttta tggattttat ggaaaaaact gatgatccta ggtttaagat
    12781 ttataaatta gcttattctg cctttctggc tcgtataacg gttaatgatg ttgtatggga
    12841 aaaggataaa ggcattaagc tgatgaatgg ttattattgg gattttatca atgatcctca
    12901 tttgcttgtt gcgggtggga ctggtggcgg taagactgtt ttgcttagat caattttaaa
    12961 atgtcttgca actattggcg ttgcggatat atgtgatcct aagcgagctg attttgtgac
    13021 aatggctgat ttacctgctt ttcgaggaag aattgttttt gaaaaagtag atattattgc
    13081 taaatttgag aatgcaatta caatcatgta tgcacgctat gactttattc gtaaggaaat
    13141 gaaacgttta gggcataaag atatggggaa attttatgat tacggattag aaccatattt
    13201 ttttatttgt gatgaatata atgccttgat gtcttcgtta tcgtatcagg aaagagacat
    13261 tgttgagaat gcctttacac agtatattct acttggccgt caagtaggct gtaatgcgat
    13321 tattgccatg caaaagccat ccgcagatga tttaccaaca aaaatcaggt caaatatgat
    13381 gcaccatatt tctgttggtc gccttgatga tggtggttat atgatgcttt ttggggatga
    13441 aaatcggaat aaagagttca ggtatattaa atttctatca ggtcaccgtg tgtatggtcg
    13501 tggctattct gctgtttttg gcgaggtagc gagagagttt tatgctcctt tgctaactaa
    13561 gaaattttca ttttatgatg aatttgcgaa aatctcacgt catgataatc catttgatcc
    13621 gcttgaaaat actttggttt ccaaggatat tatttcagat gaatcattga gaaaatttgt
    13681 agaaaaaacg tctgacaatg agttacctaa aggaagtatt ggctttgatt ttgaagagga
    13741 aatggaggaa attaatcata tgagtattgg tgatttagct aagaaaacag gtttttcatt
    13801 tggtaaaata aaaaatttaa ttgatatgat tgaaaaagat ggttacatga cttttaatcg
    13861 tgaagatgaa agagtagtat tgactgattc tgatgcagtt atattgaata gtttactcac
    13921 tcaaaaagat aagtttgatg gcagttggaa agagttgttg ttgaattatt ttaagggtga
    13981 atagtagatg aagggtattt ttttaaatat ccttctgtaa agccctagtg tagctgtagg
    14041 cgtagtgtag cgtagcggaa acggacacgc cggacacagc gcacacacta gggctttaca
    14101 gagggattta tcaattttaa aacccccgat ttctaatagg ggggtaagat ttgaaaaatc
    14161 tacgaagtaa aacccccgaa accccatatg tatcaaggat tttcaagttt caaaaatagg
    14221 ttataaaacg agcaaaaaca ggttatattt tatcaaaaaa tagctaaaaa ttgataatat
    14281 ttagggagaa aatagatgaa tccaatgtat ttaaagaaat ttaggaagaa atcaggctta
    14341 aagcagaaag aatttgcaaa atgtataggt attagtcaag ctatgttatc tcaatatgaa
    14401 tctggtaaga aaaaattatc tcttgaaaaa tttcaagaaa tgaaaacaca ttttggttat
    14461 ttgactgata atgatagttc aagattacaa gtgacaattg actatgtcag gattacgtta
    14521 aaaagcgtta gagatttgga atttttttgt cggaattttt tacattgtcg atttaaagaa
    14581 ttttcatcag ttgaatctaa gttgatgaat tataaccata tttggaaacg tggagatatt
    14641 tggatatttg attatgcaga taaacatgat acaggaaact atcaaataac attacagcta
    14701 tctggtcaag gatgtagaca attagaatta cttatggaac aagaaaattt tgcttggtta
    14761 gattggctaa tctacttaag acgttcttat cgagatgaaa tgaatgttac acgttttgac
    14821 ttagctattg atgaattgta tctgggtaag gatagagaaa atgaacactt tcttttatcg
    14881 gatatgattg ccaaatatta tcatcatgag ttagcttttg aaagtttgcg aacatggaat
    14941 tatattggtg gtggtgcttt aaattttaat gatatggaag aaattgaaca gaatcgtcaa
    15001 ggtatttcgt tatattttgg tagtcgacag tctgaaatgt attttaactt ttatgagaaa
    15061 aggtatgaat tagctaaatt agagggcatc tctgttgaag aatctttaga aatatttgaa
    15121 atatggaatc ggtatgaaat ccgattatct caatctaagg cgaatgcagt ggtagatgaa
    15181 tttatttctg gtattccatt aggagaaatt gcgagaggat tgattaatag taaaattcaa
    15241 gtttatgatg gaaaaaataa gtatggtgca tttatggctg atcaaaagtg gcagatgatg
    15301 tttggaggtg ttgaacctct aacatttgtc acgagaccag aatcttataa tattgaacgt
    15361 actattcgct ggttgattca tcaagtatct gattctctag ctatggttag tgaatatgac
    15421 agtatgattg atggggataa tctacaaatg atcattaatt ctggtgaagt gaatgaacga
    15481 ggtgaaaaaa tattagaaac tattcgatat aatctaggtt tagataaggg ggcgtagatg
    15541 tttttagaaa atgaatttaa ttattttata tcaacgagaa ataatttaga gctggtcatt
    15601 gattctttgg tattaatgat tcctgatcgt gaattttatt atcctgagat tcaaacaggt
    15661 gagcttagag attatcagat agacatttat gatttgatta agattggtta tgttggtgtt
    15721 tatgaaattc aaaaagacta cgaagataag ttaagggaac ttgctgattt taaacggaaa
    15781 cttttaaagt ttggtttgct tatgcaacct ttagaaaagc aaaaagagat agttattcgt
    15841 ttagctgcta aatatcattt ggaaaagaga attctgatga aaaaggaaat gtttagagat
    15901 gaagaagttg attaatttct gggatcattt aaaatccaac ttataaatca taaggggtaa
    15961 ggtaaatgga aaaacttgga gatcgtttaa gaaaacaacg acagcttaat aaattaacac
    16021 aacaagagct tgctgataga attggtatta atagaggagc atattcaaat tgggagaacg
    16081 gaaagcgaga accaagtttt caaaactaat tgagctatct aaactattaa atactactcc
    16141 aaattattta ttaggtattt cagatgctta aacagttagt tttagattca tgaaccttaa
    16201 gaggttcttt tttatgaggg aaaatgatga aaattaggtg tccaactatc atatgttgat
    16261 ttattggtat tgagtataaa aaaagaaaaa gaggaaaaga aaaatgtcaa atacacgttc
    16321 agaagcagat aaaaagttgt tagtggttac tcaggagcta tcagaactgc tgattagtca
    16381 tcagtatgac cagtcatggg aaaaagctgg agagttaaat agcttactta aaaaacgtga
    16441 agaattgacc ttaccaggtt atatggtcga catgattcaa caacatttga aatcatacta
    16501 ctaccaaaat aatatgatta ataaggctca taagtctatg tcagcgattg gtcataaact
    16561 acaagaattt cattaaggat gagagcaagt caatggcttg ctcctttttt aattaaggag
    16621 tgtcaaaaat ggatgttagt gcaattgtta gagatataaa aactggtaga gctacgttag
    16681 tttgttttcc tgtggaaatg agtgagctta agttagcttt ggggcttcgt gaagaagatg
    16741 atttagagta tattattgca gattcggatt gtcagttact aaaagaacat gattctattg
    16801 agatgattaa taatttgttg aactggtaga gaatgttgat agtgaacttg ttcaagcagt
    16861 gcatcaggta acaggttata ctgcctcaga ctttgtagat tatgatttta attttggtga
    16921 ttgttgttta ctgcctgatg tcacaactag acgagaatta ggcgaatact ggtttaatga
    16981 attaggttca gaaggtgttg gtaaagaaaa tatggaactc tattttgatt gtgaagctta
    17041 tggtcgagat attgacttag aaagccaagg tggtttttca gactacggat atgtcgaaat
    17101 atcagggtaa gtagataatg attgcaatta taagaactca tgaaaaaggt agaagtcaat
    17161 ttttatgtga gttagacatc atgcagttta cggaaaatca agtaagagat cacatgatag
    17221 aaagaggtat caaggatgat gcctttttca tttgtggttt ttctgattgg aatgttgata
    17281 ggattatgtc actttcggaa gtttaccttt taaagagatg tattgttggt ctttatgatg
    17341 gcgatgatta cattgttcaa tatttgttaa agaaaggctt gtcagtgcca gccatagtga
    17401 caaagtttta tgtctttttg tcaaaagatg aaaaagaagc aatgggatat gttttgaaaa
    17461 atgtatcatt tgattcattg attgattttt ggcaacggtc agtaacttgg gcaaatgctt
    17521 taaacgctta catacaaagt ggaatattgt taaatacatc caaaggattt tatgtgttaa
    17581 aaacagaagg aggttagatg gattatttag tgaaaataaa aaagtttcta ttgagatata
    17641 caaagaaaga aaaaggagga aagcagccta aaccaaagga agtaaaacaa aagacagcca
    17701 atattattgt ctatggaatt ttaggattat tatttattgt aggatttttt ggctcattaa
    17761 gggcaattgg tttgtcaaat caagtaaata gtttaaaagc tacggtttta tcaagcaagc
    17821 aacaaaacgg aaaagataca aaggaaatct tagacattcc tagagtgcag tattacatga
    17881 acaattttgt gtatacttac atcaactatt ctgatgatac ggctacaaaa cgtaaagaag
    17941 aattagataa ttattattct ttttcaacag ctaatcttgt tgatgatgtt aaaaaggagc
    18001 gtaagcttca gactcaacga ctggttagtg tagaacaaga agaggattat catattgctt
    18061 taatgagaat aggctatgag gtagatagta aatcttatca gatgacgttg gctatcccat
    18121 ttcgtatggc taatggatta ttggcaatag taagtcctcc atatacctta gcggaggatc
    18181 ttttccaagg gaaatcaaaa agttttgaaa gaaaaactgt ggatcaagtc aagagtttac
    18241 cgaagcaaga aatgttatca atacaaaaat ttttacctgt attttttgat aagtatgctt
    18301 taagtaatga aatagatctg aagttactaa tgaagcatcc tgaactgatg ggtggaaact
    18361 atcgagtcaa atctttagac gctaacaatg ctcggtatta tcaagataaa tcaaatcaag
    18421 ttgtgcaatt aatggttacc tttgaagata tggtaacagg aggtacacgg tcagagaatt
    18481 tcacgctgta tctgattaag tcagataatg gttggtatgt tgacaagttg taccattatt
    18541 ttaaataaaa aagcgaggtt taattaatga aagtatcaaa agtagctcaa gttgttttaa
    18601 caggaggagg acctaatgtt tcaggactaa aacaattttt tcagggagat gggatttatc
    18661 ttattagtgt tatctgtgct tttttagtga ttaaaaattg gaaaaatgca gattggctaa
    18721 aagttggctc agtactgtca atttatgcga ttgtggtttc tctgtttaaa gggcaagaaa
    18781 ttcttgcctt tttaggaggg atgcttcgct ggttcggtat tgagacaggt ttataaaaat
    18841 ggaagaagaa aaatattttg attacagtag gggcctaaac gccccttact ggattcaaga
    18901 aataaaaacg tcaaaaggaa agttattgtg gtatttctca acaccaatgc agctttcctt
    18961 ttttattgtt ttctttttga ttttagtatt gatgttgaca gtcttttcgg ctcctatgca
    19021 actgctaaat cagctaacgc attctatttc tctgttactg tactggtttg tcccatatcg
    19081 actttcaaaa ttttatacgg agtatgagcc acaaggtaaa aagatgcata cttatctttg
    19141 ggattatctt gtctatttaa tagattatgg ttttaataaa aaagctattt atcagggaga
    19201 aagagtagag gtactagaag aaattgtatt tgagaaaaca aacatttaaa gattaagaaa
    19261 ggagatcaga tggtaaaaaa gttggaatat ccaattaata gcattcacag taatttagct
    19321 ttgcgaaaag ataaagtggt tattgcctac tatcggattc caaatacgcc aatcactatt
    19381 actgatagtg ataaaaaaga tagtcataaa gacaaggtag cacaggttat caaaaaatta
    19441 gctaaataca agcattttga tatttcactg gtaccaaaag attacctttt agaggaaaaa
    19501 atgtctgatt ttattggtga cttggcagat gatgttaagg atttaggtat agatacattg
    19561 agttacactg ttgacacgtt gacagctgag attgaaatac cttatcaatt tgattgggtt
    19621 attggtgtta atttaggtag ggatacaatg agtgctacac taaaagaact tgcggtaaag
    19681 caattggatt taattgcaga taatgtcgcc tctttgttgg gatacactgt ggaacttgat
    19741 aaggagtggt ataaagagta ccaagatgaa gaattattgc tgtatggttt gttatcatca
    19801 ctgaaagcta agagattatc tgaagaagat ttattttatt atcaacgcat gcaatactta
    19861 cgctatatcc cacatgctaa aaatgaagtt attgccaata gaacattgtt gaacgtaaca
    19921 gatactttag ttaagcccct gtcgggaggt tttttaaagt tagaaagtcc gtatggtagt
    19981 tcatttataa cgattctacc agttgggaag tttaatacta tcttcaatgg gttccactta
    20041 ggtgaactcg tacagcggat gagttttcct gttgagttac gatttaaagc agaatttatt
    20101 aataaaacta agttaagtgg aacaatggga cgctcgaata cacgttatga tcaaattatg
    20161 aaagaagcct actccacgaa tacagtgcaa caagatgata ttctaatggg ctcatattca
    20221 ttaaaagact tgatgaagaa agttggaaat aaagaagaaa tcattgaata cggttgtttt
    20281 ttggtggttt ctgggtctag tatcaaacaa ttaaggcaac gtaggcatgc tgttttaagt
    20341 tactttgcgg atatgcaagt caatgtacat gaagctagtc atgatacacc ttatttattc
    20401 caaacgttac tttatggtca aaatttacag aagacaacac gtaaatggaa tcacttagtg
    20461 accagtcgtg ggtttagtga aatgatgtta tttacaaata cccagtcagg taacaggatt
    20521 ggctggtata ttggtcgagt cgataatcgt ttatctgctt gggataatat tggtgaagcg
    20581 attatgggct cgaagaactt agttttgttt aatgcaacgg ttgcgaataa ggaggatgtt
    20641 gctggtaaag taactaaaaa tcctcatatt atcattacag gtgcgacagg tcaggggaag
    20701 tcctaccttg cacagatgat ttttttacat actgcgcaac aaaatgttag agtgctttat
    20761 attgatccta agagagagtt aaggaaacac tatttacaag tggtgtctga tcccgagtat
    20821 gcgaaacaat ttccactgag aaaacatcag attgagcaaa ttaattttgt gacattggat
    20881 agtgcgatga aggaaaacca tggggtttta gatcctatcg ttatcttgga taaagaagat
    20941 gcctcgtcta ctgctaaaaa tatattgctt tatctgttaa gaaatgcgac agaagttaag
    21001 attgtacaaa ctactgcttt gacagaggcc attagtgaag taatctcaag acgtgaaaat
    21061 ggggaagtcg ttggctttaa tcaagtcatt gagacattat gtcaatcaga taatcaggat
    21121 attgtagatg taggacatta ttttaaagct attattaaga attctatttt ggaattggct
    21181 ttttcggatg gtaatgttaa aggattatca tatgaagagc gtgtgacagt tttagaagtt
    21241 gcggacttat cgttacctaa agatggttca gatcatatat ctgatcatga aagtaattca
    21301 atcgctttaa tgtttgcttt aggtgctttt tgtaagcact ttggggaacg aaccaatgag
    21361 gaaactgttg aaatttttga tgaggcttgg attctgatgc agtcatcaga aggtaaggcc
    21421 gttatcaagt ctatgcgccg cgttggtcgt tctaagtaca atgttttaat gttagtgtct
    21481 caatcagtac atgatgccga aaatgatgac gatacgactg gttttggtac tattttcgct
    21541 ttttacgaaa aatcagaacg ggaagctatt ttaactcatg ttggtttgga agtgaacgaa
    21601 aagaacctgg agtggattga taacatgatt tcaggacaat gcctttatta tgatgtttat
    21661 ggcaacctca atatgatgtc tgtccataac attcatccag atattgatcc cttattaaaa
    21721 ccaatgaaac agtctgtttc aagccactta gaaaataaat acgcttcgta gaaaggagag
    21781 gccaaatgga taagattgat gatggattaa tagcttctac ctttcagaaa gtcaaggatg
    21841 tggatatttt tgcattaaag gcttacatgg aaagtacaga aggtcttgag acggggttac
    21901 aagtaacttt gacagatatt tttgttaatt ttcccttttt tctacttaac ttgattgttg
    21961 gtttcttttc cattattcta cgattttttg aaaactttag cttgtataat acttatcgtc
    22021 agactgtgtt tgatagttcc aaagttttgt gggataaccc ctctaaatcc gcagatttta
    22081 cgaattcatt actttatctc ttaattgcct tatccgcttt ttccattttt ctagctttta
    22141 ttttttcaaa aggtgatttt tcaagacgat tacttcattt gtttgcggtt atgttattag
    22201 cgttcggtta ctttgggacg gtgcagtcaa cttcgggcgg tgtctatgtg ttggatatgg
    22261 ttcattcggt agctagttct tttagtgatg cagtaactaa tctatcggta gtgagtccag
    22321 aagatgagaa gcaaatcatt tctcatgagg cttcggtagc tgataattat gttatgaaaa
    22381 cgtcttatac agcttatctt tttgtaaata ctggccagtt aaatggtaag tttcataata
    22441 ataaatcagg aaaagaagag gagttagata atagcaaaat tctgggaaaa tatgataagt
    22501 caaatcagtt tgtaatacca aaaagtcaac aaatattaga gtatacaaat aaacttggtg
    22561 acggagcttt gaaaggagaa gaacaaaaca ggtggatgtc agctgttagt gactatattt
    22621 ggatcaagtc tatttatgtt attttgaaaa tttttgaagc agtgatatta gctatacctt
    22681 tattattgat acaattaatt gcttttttag cagatattgt ggtcatattg ttaatgtttt
    22741 tattcccact ggctttattg gtgtcctttt tacctaaaat gcaacatatt gcttttaata
    22801 tgcttaagct aatgcttgga ggtgtttcct ttccagctct aacaggtttt ttaactttaa
    22861 tagtctttta tatacagtca ttgattgcga catttattaa gtctcgattt acagacaatc
    22921 atttgttaag cagcagtaac cttaagggac aatctatttt atttatgttg tttattacta
    22981 ttgttgttca gggggttatt ttttggcaaa tttggaaaca taaagaaaag gtattaagtt
    23041 taattgtagg agcagacgct gcacaagcag tcacacaagc aggaaatatg gttgctcaaa
    23101 aagcgaatag tttaggagtt acaccccaaa atatctatga taaggcgcat gatatttcta
    23161 atcttgcgat gatgggagct ggttatggtg ttggtagcct tgtacatgct aaggataatc
    23221 ttagtgccat taaaaaccgt ttcaaaggag atgacacttc taattatagt gacctttcta
    23281 atgaaactaa tttcgaccat cagttttcag aattaggaga ttttgcagga gatcaagaat
    23341 tttccacaaa tagtgtcccg catgtttctg ataatggtaa tcctacctct ggacatcaag
    23401 cagaacaagg tgttttcgat aatcaagaga gtaacttttc ttctgattac aatctctctc
    23461 aagtaagtga ttttgaaaga gacaacttat acagccaatc ttttggagct tcattagata
    23521 attcagaacc cttgggtaaa cattccccaa tgaatttttc taaaccaagt cagatgactg
    23581 aatcaattga tcatgatgtt cattatgatt taccaggtgt taatgaggag atggaaagtc
    23641 catttcagtc agaaagtgat tatcaatctc aaaactcttc aattatcgtt gaaactccag
    23701 aaatggatga tatgatttat caaagcagtc agaagaaaaa tatttctatt ggaaataatt
    23761 attcctctga taaaaaatct ttggaaaatt catttgattc tccaattatt cctgaaaggt
    23821 ctgaaccaga aacggacgct aaagagagat ttaatcctca agcagaatat caacgtctaa
    23881 aagctaagcg acaaaaacct ttaggaaatc ggaaaaaatc taaattagaa gcagaattag
    23941 gtcagtttac tgatgatgcc agctattata aagcacatgg agggactgcc tttaaaagag
    24001 gatttaacgc cagtaaatca aaagagttac ggttaaaaca aaatttagaa agaaaggcga
    24061 ctattttagc tgaattagat aaacttagag gacatgttta aaggaagtat tgtgatgtta
    24121 tcaaatagtt gcttaatttg gagataagag ttataatgaa aatagaaaag ggcagttgcg
    24181 gaaacaacta cccaatgcag aaccgtttaa gacggtggca gaaaataacc gttaaaaaat
    24241 aaccgttaac tcgccaaagt tgagacggtt atttttttgt cgattttacc agttcaagga
    24301 ctaacttcac gagagcgata acgaagttac ctgctataaa catgagtgtt aagacctcaa
    24361 aggtagacaa atctagctcc tcctttctgt tggattttga tgaattgccc atgggcacca
    24421 cctacctttc agaaataaga gccaccgtct taacttttct gcctttttat tataacatac
    24481 cgtatctctg tctgaccagt ttaagaagga gggaatgtta tcaagaagaa acttggatgt
    24541 ctaggagtgc ttatgagcat tccttttttt attgtagtta tcgttacgat catatttagc
    24601 agcagtcaag gtgattgtca agtaactcct gatttatcaa cacaatttgt tacctcaagt
    24661 gattctgctt cacaaaccga ttggacaaag aaaggctcaa ttgcgaatgt cacagcaggt
    24721 aaagtctttc acgcttgggt atcgaaaggc ttgtcaggcg cttctgccgc aggaattgta
    24781 ggttgggtta attccgaagg tggttttgcc atgataggtc gagcagaagg acattatggt
    24841 aatgatttac gaactaattc tatagctttt ggagtgaaac caattggttt gagttactat
    24901 acgacagaag caggtggagg tatttatcaa tttacccctt ataccaaata tgccccttta
    24961 ggtgatccta agtgggagaa tgcggatgcc atgaacgaat ttgttgcaaa agccattcta
    25021 gctggtgatt ggaatgcttc aatggatttg acaggaggaa atcatacctt taagcaaatg
    25081 gcccagatga ccgatccgaa acaggctacg ctagtttggc aagcttatga acgtggtagt
    25141 actgcccata ttaatccatt tcaaaagcaa gcagatgctc aaaaagcgta tgagttattc
    25201 ggtggagtta aatatagtta taatgaaata gtatttcata aatcttttga catttttggt
    25261 ccactaaagc cgggggaagg ggaatcaaaa gaagtcgttt tatcattatg cgaatcaggt
    25321 tcttactcta agggggcttt tgggaaagat cgtacaggca aagtcaacta tacttcctac
    25381 aatgcttggc gaccagaaca attgcctgca gatttaaaac cttatgcgct cgatcccaga
    25441 tcagttggct tgaattatcg acaagcggtt ggctggtatg cgattgcttc gacagggggt
    25501 caatgtaccg atctttctgc gagtttgatg tatgctttgt ggtcaaaaaa tggtagccat
    25561 ccaacacaaa aggcaggtaa tggtaatatg gttgttgcta attgggttag gacttttggc
    25621 ggttcatctg ataaatcacc aacttcagga gctgtatttt cctctgcagg gactagttct
    25681 gctggccata caggtgttgt cagccatgtt tttgaaaatg gggatatttt aattgtggaa
    25741 caaaattatg catcttattc gggtgatgat ggtggctttg ggaaatactc ttggaactat
    25801 cgctatgtca ctactgcaga attaaaaaat gaaggatata ctttttacaa tcctgcgaaa
    25861 ctcggttatc agattatgaa atcagtagga acggtaggta aataatgaag ataagaaaag
    25921 ttattattgt tatgctggca gtagctcttt cattaggatt ttatcggttg tatcatcgtc
    25981 aaaagccatc tcagttaata gaagctaatc aagaaatact tgtaaaacca attacaccga
    26041 ataaaagtaa aattaaggtt aagagaaatg ttgccaaaga agccaaggcc actttagaga
    26101 tgcctgagga gcgtgttaac gaaaaagaag ctaaacaggt gaaatcagct attgtaatac
    26161 cgattgatta tttagctagt atatcaagtc aagatgatat taaggttact tatgataata
    26221 agttgtccat tactaatcca gcaatggcag caagcattaa aacgatgctt ttagcagggt
    26281 atcactttga tttagatagc ttgacggttt atcagagcaa ttctgataat gtgtatcaat
    26341 ttacgatttt attagtgact catcagcaag atcaattaag tcttgttggt aattatgtta
    26401 ctggtacggg tcagtttgaa tttgtgagtt tacatggcac tccaaaaaat gtcatgtttt
    26461 aattaaacga aaagaggaaa aataagatga caaaagaaaa aatgatgagt tttatgacga
    26521 ttgcaagtat tgctttgctt ggaagtgggg tggtttgtgc taatgatgta gcagatccag
    26581 tagcaggaac aacggatgca gtggtggtac cagtctcacc tcaaccaacg acaccgattg
    26641 agcatacagg aacagatact accaataaaa ttgttgactc gactaagcca agcgaggaca
    26701 aggtggcaga tagtactgat cagctaatcc ctccagttaa atcagattta gaaacaagcg
    26761 aggataaagt aacagatcca acggagacta agccaagcaa agagaagacg gaagagcctg
    26821 ttaagtccaa tgattcagaa gtgattccag aacaacctaa gtcagaagag gtgaaaaaag
    26881 ctgaagaaaa tacaggtgct agtacaactg aaactcctaa acctgtgacg aaacctgtta
    26941 ttgagacacc aattgttacg aatacaggct acacggtttt gggaacgcaa gatggcaatg
    27001 tttgggttca aacagaaact ggtaatgtac tgaaacctgc gatagcaatt ggtgctgtaa
    27061 agcaaaaaga tggtacagtt gccttaaaaa caagagatgg gaagttagaa gtcttaccat
    27121 caactggaga gactaaaaca atcttagcca ttttgggagg tttagctatt ttaggagcta
    27181 tctgggtagg ttggaaagaa aatatcaaaa agtatttaac gtttttgaaa aagaaaagta
    27241 aaaaataaga taaagccatg attaatttca tggctttagt tttgttgaaa ggagcgaaat
    27301 tgaaaagact agtatattat ttcatagcat taatttatag tattatttta ttgaaattaa
    27361 ttgaaacaat atctagcaga ttaatactga caatattatt actaatatta attatactac
    27421 tagaaaaagt tttgcacgat tgatataata acaaataaag ggatgttatt gtgttgaact
    27481 ataagtatat tactctaaat gatagttatg agttaaatgt ttggttagat tttgtattac
    27541 gtgatttaaa aaaggaagaa aagtattcta aatttagatt tataataaaa aaattgtcta
    27601 ttaaaaattc aagaactaca tttcaagaaa tcgaaagtgt tcgtaaagaa ttaatgggac
    27661 tatcagaact tgatttgaag catttaagaa tgataattga aaatagagag acaacaggga
    27721 ttggtttaga attcaaagca tttttacaaa agatatttaa ttattttggt gtgattgtag
    27781 taggtttaat tccagtaata ttaggttata attcaataga gagagttgta acttcttggt
    27841 taaatgccaa aacttttaaa tctagtattt tattaattgt atggatattt gttttatata
    27901 tttattatgt agtttttaaa gaattctttt tgcatgattc taaaaaaaga gaagttagaa
    27961 agtatttgtt atttttgata aatgatattt tattagatat ggagaaacgt tgatgtcatg
    28021 attagtttca tggctttttt gtttgttttt tattaaagag caaagttcgg ggatactgta
    28081 tataaattaa agctaaagat gaaaaatagt tcgattttaa attagagaac ctgcttaacc
    28141 ggaacaaata atgttcggag taattaaatt ataaggagaa tgagtatgca acaagttttt
    28201 aatgtgagtg ttcctgtacc agatgatgtt gttatcattt ctaaagagga atatttagat
    28261 ttgttaagtg ataatgaaca aggaaaatgg tgggatattg ataatttgca agaattgttg
    28321 ggccttggta ggtcaaagtt aattaatgat atcttattaa acccagatat taaaaaggaa
    28381 gttgatttat caatcaatcc aaatggtttt attgtttatc ctagggggaa aggtagccgt
    28441 tataaaatat tagcaactag agctagaaaa tactttgaag aaaattttgg ttctatttta
    28501 ttaaataact aaatgatata atattgagga taaattcctt gatgtctagg aaaggagaaa
    28561 aatggcaaca tatagacaaa gaggtaaaaa gaaattatgg gattatcgtg tttatggtaa
    28621 agatggaaaa ttaatagcat ctggttcagg ctttagaaca aaaaaagaag catcatatga
    28681 agctttaaaa attgagcaaa aaaaatatta ttcagatgtt ataaattcca atgctacatt
    28741 atttgagatg tggcaaatat ggtatgatct tgttgttgag ccaagtgaat tagatattct
    28801 tactaagcaa aaatatcaag cccgaggaaa gataattgaa aaatatttat ctgatatccc
    28861 tgctaataga attaaacata gtaagtatca agaatttatt aagttttatg gtaaaaatgt
    28921 aactttgaat ttaatgactt gtttgaattc agatattaga aaggttataa ttttttttaa
    28981 aagggacgga gttttaattg aagattttac agaaggagtt aaaatttttg gaaaaccata
    29041 taggaagtcg ccagaggata agtatttaaa aagtattgaa gattatcaaa aattattagc
    29101 atatttaaaa agaatagcta attattcaga ctcaataatc cctttttttc ttttcacaat
    29161 gttacaaact ggttttaggt ttggtgaagc aatggcaata acttgggatg atattgattt
    29221 tgaaagaaat agtatttata catatcgaag atattcttca ataaaaaagg actttacaaa
    29281 gcctaaaaca aggacttcaa taaggagagt tccagtaagt catgatttaa aagagttatt
    29341 gcttgaacta aaatttcaac aagaaagaat gctgagaggt ttgcatataa aaaatacaga
    29401 tcaattgatt ttttatgact ataggtttgg tatgatatca aataatgctg ttaacaaatt
    29461 cttggcaagc tcattggaaa aattagaaat agaatctaag atgtcatcaa caggtgctag
    29521 acatacttat ggaagttatt tgttggctaa cggagtagat atttgggcgg ttgcaaaatt
    29581 aatggggcat aaagatatca aacaattaat agaaacatat ggtcatttgc ttctggaggt
    29641 ggaagaaaag gaaaatgatc agcttttaaa aattcttaat aataaaggta gtttttaaaa
    29701 tgactatcaa gaacaaatta agaacaaaaa taaaagaaac cctattataa taaggtttca
    29761 ctcgtatatt ttctgccacc accgagaatc gaactcggaa cgaagcatta ccattgcttt
    29821 attataccat ttaactacag cggctacctt cttataatac tataagttat taattgatgc
    29881 aagcttttct taaaaatatt tccttaattg tttatcatca aaatgagagt atcagtta
//
LOCUS       DAUVUY010000010        29405 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.010, whole genome shotgun sequence.
ACCESSION   DAUVUY010000010 DAUVUY010000000
VERSION     DAUVUY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29405
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.010"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..104)
                     /locus_tag="VOX58_000462"
     CDS             complement(<1..104)
                     /locus_tag="VOX58_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease"
                     /protein_id="HES5877720.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVA"
     gene            338..1519
                     /gene="lctO"
                     /locus_tag="VOX58_000463"
     CDS             338..1519
                     /gene="lctO"
                     /locus_tag="VOX58_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HES5877721.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYCSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDHKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            1785..6722
                     /gene="scpC"
                     /locus_tag="VOX58_000464"
     CDS             1785..6722
                     /gene="scpC"
                     /locus_tag="VOX58_000464"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HES5877722.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLMMTTTVAADELTTTSEPT
                     ITNHAQQQAQHLTNTELSSAESQSPDTSQITPKINREKEQPQGLVSEPTTTELADTDA
                     APMANTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFEDFD
                     EDWENFEFDAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKYES
                     HGMHVTGIVAGNSKEAAATGERFLGIAPETQVMFMRVFANDVMGSAESLFIKAIEDAV
                     ALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDPLA
                     TNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVDFK
                     DIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIERDPNKTYDEMIALAKKHGAL
                     GVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVSKA
                     PSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIAGA
                     SLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNIDG
                     AVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKAKTLRYDTELLTDHVDPQKG
                     RFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRFRD
                     SQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGKHF
                     TGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNNQD
                     FAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTLLG
                     TAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDPET
                     NRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQADG
                     SFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKETL
                     KDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKGLK
                     NNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQYV
                     VTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVREEV
                     FYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVEDRA
                     GNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYYNN
                     SGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQIT
                     AHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRIEG
                     NTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKTTTSATAK
                     ALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            7495..8202
                     /locus_tag="VOX58_000465"
     CDS             7495..8202
                     /locus_tag="VOX58_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HES5877723.1"
                     /translation="MSKKKRKHYQVYDGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSVIL
                     TGIAFGMALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            8442..10442
                     /gene="metG"
                     /locus_tag="VOX58_000466"
     CDS             8442..10442
                     /gene="metG"
                     /locus_tag="VOX58_000466"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HES5877724.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEEVFRDEDGKVIGGIAPSGH
                     QVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADFSQLIDAQLADYHKHMEAVDYPRALEAIWTIIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKCYPNEQELVGKKLQIVA
                     NLKPRKMMKQYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            10933..11946
                     /gene="nrdF"
                     /locus_tag="VOX58_000467"
     CDS             10933..11946
                     /gene="nrdF"
                     /locus_tag="VOX58_000467"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HES5877725.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRSRVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYDSPFTDEETRISPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            11950..12438
                     /gene="nrdI"
                     /locus_tag="VOX58_000468"
     CDS             11950..12438
                     /gene="nrdI"
                     /locus_tag="VOX58_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HES5877726.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQINNLITERTHPCHKPMRHT
                     SH"
     gene            12405..14585
                     /gene="nrdE"
                     /locus_tag="VOX58_000469"
     CDS             12405..14585
                     /gene="nrdE"
                     /locus_tag="VOX58_000469"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HES5877727.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECQSCMI"
     gene            complement(14790..15542)
                     /locus_tag="VOX58_000470"
     CDS             complement(14790..15542)
                     /locus_tag="VOX58_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HES5877728.1"
                     /translation="MLKKRYQLAMILLLSCFSLVWQTEGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLVYPRAEQEAMAAYTCQQAAPINTSLDKTKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDQKKLHIEAYFKGSL"
     gene            15999..16451
                     /locus_tag="VOX58_000471"
     CDS             15999..16451
                     /locus_tag="VOX58_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877729.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMTRPVKAEVSEIGHDHVTIS
                     SNGQTDEGAAYGRGHDDGSKFGYEAGLQSSWNESEPPSSDKIPEPSVNPYESSNEQDR
                     EDYKEGFRDGYPGGYVAGWRKTHPIEATLQYLWYTVSSWFESLFNNSK"
     gene            complement(16758..18010)
                     /locus_tag="VOX58_000472"
                     /pseudo
     CDS             complement(16758..18010)
                     /locus_tag="VOX58_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            18466..18939
                     /locus_tag="VOX58_000473"
     CDS             18466..18939
                     /locus_tag="VOX58_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877730.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMGQPIKAEAVVSDLNDAVSS
                     MGDTAEETDVNIDGQEDEEDEEDDAYMQGRRLGEKEGYEAGQKDNAPRLFDSSTLEPP
                     TNPYEDAGDTLNSNWFKSGYSDAYGQAYEQGWNDTHPIQSTLIWLWGIVRNLFRF"
     gene            19192..19443
                     /locus_tag="VOX58_000474"
     CDS             19192..19443
                     /locus_tag="VOX58_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877731.1"
                     /translation="MPEKKVLPVLSMVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     SLVQNKGRQKVWSLAGLILAALAVAVVAYAYYDPTVIPY"
     gene            complement(19816..20316)
                     /locus_tag="VOX58_000475"
     CDS             complement(19816..20316)
                     /locus_tag="VOX58_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877732.1"
                     /translation="MKKTLTLLLALFTIGVTSSVRAEESSWVPLPRQQKVLDDNRNEI
                     PVHITGNKVTVTLPPGWSAWIDKNTKQRNSNSVTTTVTLDISTSQPPKEFTIYFQGDD
                     GFLAGMMTFIDSETLKNEQYKAFDEEYAENLFENSRLREEQKTWRDKVRDVFQDAWWN
                     FKGLFQ"
     gene            complement(20584..21282)
                     /gene="spej"
                     /locus_tag="VOX58_000476"
     CDS             complement(20584..21282)
                     /gene="spej"
                     /locus_tag="VOX58_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeJ"
                     /protein_id="HES5877733.1"
                     /translation="MKRLIKTIILVIIIFHGYGSVKSDSEKIKDVKLQLNYAYEIRPV
                     DYTNCNIDYLTTHDFYIDVSSYKKKNFSVDSEVESYITTKFTKNQKVNIFGLPYIFTR
                     YDVYYIYGGVTPSVNSNSENSKIVGNLLIDGVQQKTLINPIKIDKRIFTIQEFDFKIR
                     QYLMQTYKIYDPNSPYIKGQLEIAINGNKHESFNLYDATSSSTRSDIFKKYKDNKTIN
                     MKDFSHFDIYLWTK"
     gene            complement(21484..21621)
                     /locus_tag="VOX58_000477"
     CDS             complement(21484..21621)
                     /locus_tag="VOX58_000477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877734.1"
                     /translation="MLTYHELKKAVANRYIKGDMISITRKLVSSLILSFLIELYEYQE
                     F"
     gene            21853..22136
                     /locus_tag="VOX58_000478"
                     /pseudo
     CDS             21853..22136
                     /locus_tag="VOX58_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22310..22813
                     /locus_tag="VOX58_000479"
     CDS             22310..22813
                     /locus_tag="VOX58_000479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877735.1"
                     /translation="MKTRPKRFLNLATLSLALLGTTLLMAQPVKADNDTRESQERVDQ
                     RIEDPYDDGKNAGRKDGYESGNHKGISEEPPTKTPEPPTNPYSRDRVKSKKYRDGYQE
                     WYVYGYYEGWHRANDEDKNKESSDHSRDQDLEGTEQTDTEYSAIISTLYGIAGVVLEY
                     VLSWFSI"
     gene            23473..23709
                     /locus_tag="VOX58_000480"
     CDS             23473..23709
                     /locus_tag="VOX58_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HES5877736.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            23702..24400
                     /locus_tag="VOX58_000481"
     CDS             23702..24400
                     /locus_tag="VOX58_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HES5877737.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLRQGYHVYGVDKSDKPDLSGNF
                     HFIKLDLSSELSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLAEWVARETPIGRWTEPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            24476..25435
                     /locus_tag="VOX58_000482"
     CDS             24476..25435
                     /locus_tag="VOX58_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HES5877738.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLATSEYEAVLGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLNNMTFIKEHLDQLGDIKIVECNYSQYSSRYDVF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKTTIQGNKGSLAVLGATNTLPQVQLSLHGHEAQVVNHNKY
                     DHRMYEEFVAFRDMIDQRDFEKVNQALEHSRAVMAVLERAVHS"
     gene            25768..27105
                     /locus_tag="VOX58_000483"
     CDS             25768..27105
                     /locus_tag="VOX58_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HES5877739.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISMLEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            27278..28660
                     /gene="glmU"
                     /locus_tag="VOX58_000484"
     CDS             27278..28660
                     /gene="glmU"
                     /locus_tag="VOX58_000484"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HES5877740.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVTIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGDNCVVTNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDHALTAAGSTISKTVPIDSIAIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            28691..29245
                     /locus_tag="VOX58_000485"
     CDS             28691..29245
                     /locus_tag="VOX58_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HES5877741.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKILLVKQYRKAIERVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            29245..>29405
                     /locus_tag="VOX58_000486"
     CDS             29245..>29405
                     /locus_tag="VOX58_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877742.1"
                     /translation="MGRPLLTDDIIEKARRMETFESDDAVDFDTKVMTLPEKDDKARI
                     YKSRRIENA"
BASE COUNT         8940 a         5760 c         6275 g         8430 t
ORIGIN      
        1 tgagcgacta aagtgtctaa aacagcgcgt gataaaagcg cacgagggga ctctcctgtg
       61 agagcagcat ttaaggaatc aatgttccat gaaatcagtt tcatttggtt tctctttcta
      121 acagatagtc ttgttgttat tgtaacaaaa aacgaaacga taagcgactt tgggcaccta
      181 gcccccataa aacagtctag ccaaatgaag catagcagcg taagaacaag acaaggaggt
      241 gatcagtgag catatcgttt gacagttaag agtaggatta ctataataga aacataaatg
      301 taagcgctaa caaaataaaa aatataggag aatgtcaatg gcacaaaaaa cagtcatcac
      361 cgaagaaaca accgattttg ttatggattt caaaacaagt agcgctgagg gaaatgttga
      421 ttttattaac gtttttgacc ttgagaaaat ggcacaacag gtcatcccta aaggagcctt
      481 tggttatatt gctagtggtg ctggtgatac ctttaccctc catgaaaata ttcgctcatt
      541 taatcataaa ttaattgtcc ctcactccct caaaggggtt gaaaatccgt caacagagat
      601 tacgtttgat ggtgatcact taacctctcc tcttatttta gcacctgtag cggctcataa
      661 gctggccaat gaacaaggag aagtagcgtc tgccaaagga ttgaaggaat ttggaagtat
      721 ctacaccact agttcctact caacaactga cctgccagaa atcagtgctg ccttaggtgg
      781 gacaccccat tggtttcaat tttattgcag taaagacgat ggcattaatc gtaatattat
      841 ggatcgtgtc aaagcacaag gttgcaaagc tatcgttttg acagcagatg caacagttgg
      901 aggaaaccgc gaagttgata ggcgaaacgg ctttgttttt ccggtcggta tgccaattgt
      961 tcaagaatat ttacctgatg gtgcaggtaa aacaatggat tatgtctata aatcagctaa
     1021 acaagcgctt acctcaaaag atattgaata tatcgcaacc tactcaggcc tgcctgttta
     1081 tgtcaaagga ccacagtgtg ctgaggatac cttgcgggca ttggacgcag gagcatcagg
     1141 tatctgggta accaatcatg gtggccgtca attagatggt ggtccagctg cttttgattc
     1201 cttacaagaa gtcgctgaag cagttgatca caaagttcct attgtctttg actcaggtat
     1261 aagacggggt caacatattt tcaaagctct tgcttcaggt gctgaccttg ttgccttggg
     1321 gcgtccagcg atttacggct tggctatggg aggaagcatt ggtactcgtc aggtctttga
     1381 aaagttaaat gacgagttaa aaatggttat gcaactggct gggacacaaa ctatccaaga
     1441 tgttaaagcc tttaaccttc gccacaatcc gtatgattca tcgattccat ttgatcaaaa
     1501 tgcactacgc ttagattaga acagacaaaa acctctgcta tcatgtcaca aatagtagag
     1561 gttttacact gcaaaaagag gtatgaaaat ccaataacat agttacaagg ctttcgttta
     1621 atacccccca ctaatagatt aaaatgaaag acttcttaca ttaatagaag tttctgttgc
     1681 ttttctcaga aaaatgtggt taaataatca aatattatta acgaaaaatg agttaatgat
     1741 taacattaaa ctggttttag ttaaaacatt taggagggta tcaggtggag aaaaagcaac
     1801 gtttttccct tagaaaatac aaatcaggaa cgttttcggt cttaatagga agcgttttct
     1861 tgatgatgac aacaacagta gcagcagatg agctaaccac aacgagtgaa ccaacaatca
     1921 cgaatcacgc tcaacaacaa gcgcaacatc tcaccaatac agagttgagc tcagctgaat
     1981 cacaatcccc agacacatca caaatcactc ccaagataaa tcgtgaaaaa gagcaaccac
     2041 aaggtctagt ctctgagcca accacaactg agctagctga cacagatgca gcaccaatgg
     2101 ctaatacagg tcctgatgcg actcaaaaaa gcgcttcttt accgccagtc aatacagatg
     2161 ttcacgattg ggtaaaaacc aaaggagctt gggacaaggg gtacaaagga caaggtaagg
     2221 ttgtcgcagt tattgacaca gggatcgatc cggcccatca aagcatgcgc atcagtgatg
     2281 tatcaactgc taaagtaaaa tcaaaagaag acatgctagc acgccaaaaa gccgccggta
     2341 ttaattatgg gagttggata aatgataaag ttgtttttgc acataattat gtggaaaata
     2401 gcgataatat caaagaaaat caattcgagg attttgatga ggactgggaa aactttgagt
     2461 ttgatgcaga gccaaaagcc atcaaaaaac acaagatcta tcgtccccaa tcaacccagg
     2521 caccgaaaga aactgttatc aaaacagaag aaacagatgg ttcacatgat attgactgga
     2581 cacaaacaga cgatgacacc aaatacgagt cacacggtat gcatgtgaca ggtattgtag
     2641 ccggtaatag taaagaagcc gctgctactg gagaacgctt tttaggaatt gcaccagaga
     2701 cccaagtcat gttcatgcgt gtttttgcca acgacgtcat gggatcagct gaatcactct
     2761 ttatcaaagc tatcgaagat gccgtggcct taggagcaga tgtgatcaac ctgagtcttg
     2821 ggaccgctaa tggtgcacag cttagtggca gcaagcctct aatggaagca attgaaaaag
     2881 ctaaaaaagc cggtgtatca gttgttgtag cagcaggaaa tgagcgcgtc tatggatctg
     2941 accatgatga tccattggca acaaatccag actatggttt ggtcggttct ccctcaacag
     3001 gtcgaacacc aacatcagtg gcagctataa acagtaagtg ggtgattcaa cgtctaatga
     3061 cggtcaaaga attagaaaac cgtgccgatt taaaccatgg taaagccatc tattcagagt
     3121 ctgtcgactt taaagacata aaagatagcc taggttatga taaatcgcat caatttgctt
     3181 atgtcaaaga gtcaactgat gcgggttata acgcacaaga cgttaaaggt aaaattgctt
     3241 taattgaacg tgatcccaat aaaacctatg acgaaatgat tgctttggct aagaaacatg
     3301 gagccctggg agtacttatt tttaataaca agcctggtca atcaaaccgc tcaatgcgcc
     3361 taacagctaa tgggatgggg ataccatctg ctttcatatc gcacgaattt ggtaaggcca
     3421 tgtcccaatt aaatggcaat ggtacaggaa gtttagagtt tgacagtgtg gtctcaaaag
     3481 caccgagtca aaaaggcaat gaaatgaatc atttttcaaa ttggggccta acttctgatg
     3541 gctatttaaa acctgacatt actgcaccag ggggtgatat ctactctacc tataacgata
     3601 accactatgg tagccaaaca ggaacaagta tggcctctcc tcagattgct ggcgccagcc
     3661 ttttggtcaa acaataccta gaaaagactc agccaaactt gccaaaagaa aaaattgctg
     3721 atatcgttaa gaacctattg atgagcaatg ctcaaattca tgttaatcca gagacaaaaa
     3781 cgaccacctc accgcgtcag caaggggcag gattacttaa tattgacgga gctgtcacta
     3841 gcggccttta tgtgacagga aaagacaact atggcagtat atcattaggc aacatcacag
     3901 atacgatgac gtttgatgtg actgttcaca acctaagcaa taaagccaaa acattacgtt
     3961 atgacacaga attgctaaca gatcatgtag acccacaaaa gggccgcttc actttgactt
     4021 ctcgctcctt aaaaacgtac caaggaggag aagttacagt cccagccaat ggaaaagtga
     4081 ctgtaagggt taccatggat gtctcacagt tcacaaaaga gctaacaaaa cagatgccaa
     4141 atggttacta tctagaaggt tttgtccgct ttagagatag tcaagatgac caactaaata
     4201 gagtaaacat tccttttgtt ggttttaaag ggcaatttga aaacttagca gttgcagaag
     4261 agtccattta caggttaaaa tctcaaggca aaactggttt ttactttgat gaatcaggtc
     4321 caaaagacga tatctatgtc ggtaaacact ttacaggact cgtcactctt ggttcagaga
     4381 ccaatgtgtc aaccaaaacg atttctgaca atggtctaca cacacttggc acctttaaaa
     4441 atgcagatgg caaatttatc ttagaaaaaa atgcccaagg aaaccctgtc ttagccattt
     4501 ctccaaatgg tgataacaac caagattttg cagccttcaa aggtgttttc ttgagaaaat
     4561 atcaaggctt aaaagcaagt gtctaccatg ctagtgacaa ggaacacaaa aatccactgt
     4621 gggtcagccc agaaagcttt aaaggagata aaaactttaa tagtgacatt agatttgcaa
     4681 aatcgacgac cctgttaggc acagcgtttt ctggaaaatc gttaacagga gctgaattac
     4741 cagatgggta ttatcattat gtggtgtctt attacccaga tgtggttggt gccaaacgtc
     4801 aagaaatgac atttgacatg attttagacc gacaaaaacc ggtactatca caagcaacat
     4861 ttgatcctga aacaaaccga ttcaaaccag aacccctaaa agaccgtgga ttagctggtg
     4921 ttcgcaaaga cagtgtcttt tatctagaaa gaaaagacaa caagccttat acagttacga
     4981 taaacgatag ctacaaatat gtctcagtag aagacaataa aacatttgtg gagcgacaag
     5041 ctgatggcag ctttatcttg ccgcttgata aagcaaaatt aggggatttc tattacatgg
     5101 tcgaggattt tgcagggaac gtggccatcg ctaagttagg agatcaccta ccacaaacat
     5161 taggtaaaac accaattaaa cttaagctta cagacggtaa ttatcagacc aaagaaacgc
     5221 ttaaagataa tcttgaaatg acacagtctg acacaggcct agtcacaaat caagcccagc
     5281 tagcagtggt gcaccgcaat cagccgcaaa gccagctaac aaagatgaat caggatttct
     5341 ttatctcacc aaacgaagat gggaataaag acttcgtggc ctttaaaggc ttgaaaaata
     5401 acgtgtataa tgacttaacg gttaacgtat acgctaaaga tgaccaccaa aaacaaaccc
     5461 ctatctggtc tagtcaagca ggcgctagtg catccgctat tgaaagtaca gcctggtatg
     5521 gcataacagc ccgaggaagc aaggtgatgc caggtgatta tcagtatgtt gtgacctatc
     5581 gtgacgaaca tggtaaagaa catcaaaagc agtacaccat atctgtgaat gacaaaaaac
     5641 caatgatcac tcagggacgt tttgatacca ttaatggcgt tgaccacttt actcctgaca
     5701 agacaaaagc ccttggctca tcaggcattg tccgcgaaga agtcttttac ttggccaaga
     5761 aaaatggccg taaatttgat gtgacagaag gtaaagatgg tatcacagtt agtgacaata
     5821 aggtgtatat ccctaaaaat ccagatggtt cttacaccat ttcaaaaaga gatggtgtca
     5881 cactgtcaga ttattactac cttgtcgaag atagagctgg taatgtatct tttgctacct
     5941 tgcgtgacct aaaagcggtc ggaaaagaca aagcagtagt caactttgga ttagacttac
     6001 cggtccctga agacaaacaa atagtgaact ttacctacct tgtgcgggat gcagatggta
     6061 aaccgattga aaacctagag tattataata actcaggtaa cagtcttatc ttgccatatg
     6121 gcaaatacac ggtcgaattg ttgacctatg acaccaatgc agccaaacta gagtcagata
     6181 aaatcgtttc ctttaccttg tcagctgata acaacttcca gcaagttacc tttaagatga
     6241 cgatgttagc aacctctcaa ataactgccc actttgatca tcttttgcca gaaggcagtc
     6301 gcgttagcct taaaacagct caaggtcagc taatcccgct tgaacagtcc ttgtatgtgc
     6361 ctaaagctta tggcaaaacc gttcaagaag gcacttacga agttgttgtc agcctgccta
     6421 aaggctaccg tatcgaaggc aacacaaagg tgaataccct accaaatgaa gtgcacgaac
     6481 tatcattacg ccttgtcaaa gtaggagatg cctcagattc aactggtgat cataaggtta
     6541 tgtcaaaaaa taattcacag gctttgacag cctctgccac accaaccaag acaacgacct
     6601 cagcaacagc aaaagcccta ccatcagcgg gtgaaaaaat gggtctcaag ttgcgcatag
     6661 taggtcttgt gttactcgga cttacttgcg tctttagccg aaaaaaatca accaaagatt
     6721 gataacaaaa cgcaaagaga caacaaatca tcccttttaa cagtcggacc tttctttgag
     6781 attgagctgg cagatgatca tttcttaaca aaagttaaag agttgagcaa agtaaggaac
     6841 agacataaag accataaaag caagccagtt tggcttgctt ttatggtgtt attgtcgtgt
     6901 gctacgatga ctgaaaaaga acaacgaaca atgacctcga atcaccaaaa ggacttgatt
     6961 tgatagcttc cctatgctat actaagccta ttctttcaag aaggactcct tttgtggaaa
     7021 agactgaaaa aaaggacact aatctatcca aaaactccag atgagtagct gcaaacggat
     7081 gcctctgctg tgatcaaggg gataaagcag actggcttgt cactacctga gcaggtgtca
     7141 aaaaaagaag cttttggctt tgacattcaa catactgagc tttagtattt tagaacggag
     7201 acacccccag ttttgtactc aaaaagcatg aacagttctg ctgatgtgac atctgatttc
     7261 tacgtgcgca gtgagccaaa acagctgctg tttgctatgg aaaaagtggc gccaatcgag
     7321 atttttgaga ccctaggctc aaaaataagg catagaattc caaaggaagt cgctgccgtg
     7381 agcacaacct aagaaaaact attccaaaag actttttctg caatttgaaa tactgaattt
     7441 cagtattttt tcttttgtaa ctaagagcaa tgaatgaggt aaaaaaaggc aacaatgtca
     7501 aaaaagaaac gaaaacacta tcaggtttat gatggattac gctgtgctat gacgctttgt
     7561 tttatcagtg gctatgtcaa tgcctttacc tatatgaccc aaggcaaacg ttttgcaggt
     7621 gttcagacag gcaacctttt gtcatttgcc attcgcttgt cagaacagca actgaaggag
     7681 gcactgcaat ttttacttcc catgatagtt tttatgcttg ggcagtcctt tacttatttc
     7741 atgcaccgct gggccactaa aaaagggctc cactggtact tattatcaag cgttatctta
     7801 acagggattg cttttggaat ggccctattc acgccttttc ttccttccaa tgttacggta
     7861 gcagctttag ccttttttgc ctctattcag gttgatacgt ttaaaacctt acgaggagct
     7921 agctatgcta atgtgatgat gacaggaaat atcaaaaatg ccgcctattt actaaccaaa
     7981 ggcttgtacg aaaaaaatca cgaactaaca catatcggac gcaacacctt aatagtcatc
     8041 ttggcttttg ctgttggggt ggtttgttcg acgttgcttt gcatagctta tggcgagtat
     8101 gctttgatgc ctattttaat gcctttactc tatgtcaatt acctgttggc tcaggaattt
     8161 tatcacattc aaaccaaaat caaaccgatt aaaagaactt aacggacttg tggagaaaga
     8221 aagttcattt agggtaagtt tttacttacc ctccgttgat ttgctggttt tgctgcccct
     8281 taaaatatga tataatagac cattgatagc tgttacttaa cagaaggctg ataaaaaagg
     8341 ttccattagc cttgagggtt gaatttaaac aggaaaaaac gaaatcctac actatgcatc
     8401 tcaatcgtac tttcatttga gtatggttct gaaaggaaac tatgatgaaa aaaccatttt
     8461 acattacgac acccatttat tacccatctg ggaaattaca cattggttct gcctatacaa
     8521 ctattgcttg tgatgtcttg gcacgctaca agcgtctgat gggccatgag gtcttttacc
     8581 tcacaggact agatgaacac ggacaaaaga ttcaaaccaa ggcaaaagaa gctggtatta
     8641 cacctcaaac ctacgttgat aacatggcaa aggatgtcaa agccctttgg caattgctag
     8701 atatttccta tgacacattt atccggacaa cggacgatta ccacgaagag gtcgttgctg
     8761 ctgtttttga aaaattactg gcgcaagatg acatttacct tggtgaatac tctggttggt
     8821 attctgtctc tgatgaggaa tttttcactg aaagccaact agaagaagtt tttagagatg
     8881 aagacggtaa ggttattgga gggattgctc cttcaggtca ccaagtagag tgggtatcag
     8941 aagaatctta cttcctacgc cttagcaagt acgctgaccg cctcgtagcc ttctttaaag
     9001 agcgacctga ctttatccaa ccagacggtc gtatgaatga aatggtgaaa aactttatcg
     9061 agccaggcct agaagatttg gctgttagcc gtactacctt tacatggggt gtacctgttc
     9121 catcagaccc aaaacacgtg gtttatgtct ggatcgatgc cctcctaaac tacgcgacag
     9181 ctcttggtta tggtcaagct aatcacgcta actttgataa attctggaat ggaacggttt
     9241 tccacatggt tggaaaagat atcttgcgtt ttcatagcat ctactggccg atcttactca
     9301 tgatgcttga tttaccaatg ccagaccgct taattgccca tggttggttt gtcatgaaag
     9361 acggtaaaat gtctaaatca aaaggcaatg tggtctatcc agaaatgtta gtggagcgct
     9421 ttggacttga tcctcttcgt tattacctca tgcgttcttt gccagttggt tcagacggga
     9481 cctttacccc tgaagattat gtgggtcgta tcaactatga gttagccaac gaccttggca
     9541 atttattgaa ccgtaccgtt gccatgatca acaagtattt tgacggcacg gtgccagctt
     9601 atgttgacaa tggaactgcc tttgatgctg acttcagtca actgattgat gctcagctag
     9661 cagactacca taagcacatg gaagctgtag actacccacg cgccttagaa gccatttgga
     9721 ccatcattgc tcgtaccaac aaatacatcg atgaaaccgc tccttgggtg ctcgctaaag
     9781 aagatggtga caaagctcaa ctagccagtg tcatggcaca cttggcagct agcttgcgcg
     9841 tggtcgctca tgtgatccaa ccctttatga tggaaacatc agctgctatc atggcccagc
     9901 ttggcttagc gcctgtttct gacttatcaa cacttgctct agcagatttt cctgccaata
     9961 caaaggtcgt tgccaagggc acaccgattt tcccacgtct tgacatggaa gctgagattg
    10021 actacattaa agcccaaatg ggtgacagct cagctatttc ccaagaaaaa gaatgggtcc
    10081 ctgaagaggt tgcgctcaaa tctgaaaaag acgtcatcac atttgaaacc tttgatgctg
    10141 tggaaatccg tgtggcagaa gtcaaggaag tgtcaaaagt ggaaggttca gaaaaattgc
    10201 tgcgctttag agtagatgca ggagacgggc aagaccgtca aatcttgtca ggtattgcca
    10261 aatgctaccc caacgagcaa gaattagtgg gtaaaaaact acaaattgtt gctaacctga
    10321 aaccgcgtaa aatgatgaaa caatacatca gccaaggcat gattttgtca gcagagcatg
    10381 gagatcagct aacggtctta accgttgatt cctctgttcc aaatggaagt atcattggct
    10441 gatgctgacc tattattcaa aaacctaagc aagaatatgt gcttaggttt tttgattgcc
    10501 ttcaagaaag atgaataacg agatgggtga tatggcatca aaagcataac tatcctagga
    10561 cctgtcctag taagattgct gtgcttttga caagtgaaca gagaggtctt aaactatatc
    10621 ttgtcataaa aaagattgca aaacactata tcttgtgtta ttatggtttt tataaaagtt
    10681 aagcgtgctt aactttagcc aaacaaaaga ggacactaga gcgtgtccca caagacagtt
    10741 gagcccttcg cttttttagt agagtaggag tgctcaagtc aaaaagagac agcccatcaa
    10801 accattgctg tttagcaggt ctttttgtgt tgctcaaaaa acagttcctg tctgtttggc
    10861 gtctagtgat gacttaggct tagcgctact agacttttgt ttgaaaaata cctcaagcct
    10921 tgaaggagac aaatgaccca acattattat gaacgttcgc aatccccaat tgaatacgcc
    10981 ctatctgaaa cacaaaaaca gttgcgctcc atcaattgga attaccttaa tgatgacaaa
    11041 gatttggaag tgtggaaccg tgtcactcaa aatttttggt tgcctgaaaa ggttcctgtt
    11101 tctaatgatt taaattcttg gcgctccttg ggtgaggatt ggcaacagct catcacaagg
    11161 acctacactg ggttgacctt actagataca gtacaagcaa cggttggtga tgtggcacaa
    11221 attcagcatt cacaaaccga ccacgaacaa gtcatctata cgaactttgc ctttatggtg
    11281 gggattcatg cccgctctta cgggacgatt ttttcgaccc tttgcagcag cgagcaaatc
    11341 gaagaagcac atgaatgggt ggtctcaaca cagagcttgc aagaccgttc ccgtgtcttg
    11401 attccttact atacgggtga tgaccctttg aaatcaaaag tagcagcagc tatgatgcca
    11461 ggatttttgc tgtatggcgg cttttacctt cctttttacc tatcagcacg tggtaaaatg
    11521 ccaaacacct ctgatatcat ccgcctgatt ttacgcgaca aagttattca caactattac
    11581 agcggttaca aataccaaca aaaagtagca cgtcttagcc cagaaaaaca ggctgaaatg
    11641 aaggcctttg tctttgactt gctctatgaa ctcatcgacc ttgaaaaagc ttacttgaga
    11701 gaactgtatg caggctttga tttggcagaa gatgctatcc gttttagcct ttataacgct
    11761 ggaaaattcc ttcaaaattt aggttatgat tcgcccttca cagatgaaga aacccgtatc
    11821 tcacctgaag tatttgctca gctatcagcg cgtgcagacg aaaaccatga ctttttctca
    11881 ggcaatggct catcttatgt gatggggatc accgaagaaa ccacagatga cgattgggag
    11941 ttttaagaaa tggcagagct gattattgtg tatttttcat ctaagtccaa taacacccat
    12001 cgcttcgtcc aaaaattagg cctgcctgct cagcgcatac cagtagataa taggccactt
    12061 gaagtttcaa cccattacct tttgattgtg cctacctatg cagctggtgg ttcagatgcc
    12121 aaaggagccg ttcctaaaca agtgattcgt tttttgaata atcctaacaa tcgtaaacat
    12181 tgtaagggag tgatctcttc aggaaatacc aattttggcg atacctttgc cttagcaggc
    12241 cctatcattt ctcaaaaatt gcaggtcccc ctattacacc aatttgaact attaggcact
    12301 gctacagatg tcaaaaaggt acaggcgatc tttgcccgct taaagcatca cacacacgac
    12361 aagcaaaaac aaatcaacaa cctgattaca gaaaggacac acccatgtca caaaccaatg
    12421 cgtcatacct ctcattaaat gcactgaccc gttttaaaaa accagacggc agctaccatt
    12481 ttgatagcga taaagaagct gttaggcgct acctggaaga acatgtctct cccaatcaaa
    12541 tggcttttaa ctcactagaa gataagctag cttacctcat caatgaaggg tattatgagc
    12601 aggccatttt tgatgcctat cctaacgacc ttatcaagga ggcctttcat tatgcctatc
    12661 aacaaggcta tcgcttttta aatctcatgg gcgctatgaa attttaccag tcctatgcct
    12721 taaaaacctt ggacggtaag cagtatctag aaacatttga agaccgtgct gtgatgaatg
    12781 cactcttttt agctgatgga gatcaaacct ttgtctttga cgtgattgat gccatcttgc
    12841 accgacgctt tcaaccagct accccaacgt ttttaaatgc cggaaaaaaa cgccgtggcg
    12901 aatacatttc gtgctatctt cttcgtgtcg aagacaatat ggaatctatc tctcgcgcca
    12961 ttagcaccag cttgcaactc tcaaaaagag gaggcggagt cgccttgtgc ctcaccaacc
    13021 tcagagaaat cggagcaccg ataaaaggga ttgaaaatca ggcaacaggc atcgttcctg
    13081 tcatgaaact actagaggac tctttttcct atgccaatca actaggtcag cgccaaggag
    13141 caggtgcggt ttaccttcat gcccatcatc ctgaggtctt gaccttttta gacaccaaac
    13201 gtgaaaatgc cgatgagaaa attcgcatta agtccctagc attgggactt gtgatcccag
    13261 atattacctt ccagttagcc aaagaaaaca aagacatggc tctcttttca ccctatgata
    13321 tcaagcgtgc ctatggcaag gacatgtcag acatctccat cacagaggaa tacgataaac
    13381 tgctagccaa tccagccatc aaaaagacct acatcagtgc tagaaagttc tttcagttaa
    13441 ttgcagagct acattttgag tcaggctacc cttacctctt gtttgatgac acggtcaata
    13501 agcgcaatcc tcatgccaaa aaaggcagaa tcgtcatgtc taacctgtgt tctgaaatcg
    13561 ctcaagtcag caccccaagc actttcaaag aagatttgag ttttgaaacc attggagagg
    13621 atatctgctg taatttaggg tctatcaata ttgcccaggc tatggcagat gcgccacact
    13681 ttgaacagct catcacaacg agcatcagag cacttgatcg tgtctctaga gtgtctgacc
    13741 tgaattgtgc gccttctgtt gagacaggca atgcagccaa ccatgcagta ggattaggcg
    13801 ccatgaacct acatgggttt ttggcaacca accacatcta ctatgataca aaagaagccg
    13861 ttgactttac agatctgttt ttccatgcca tggcctatta tgcttttaaa gcctcttgcc
    13921 aattagccaa agaaaaaggc gcttttgcag gattttcgtt atcaacctat gcagatggca
    13981 cttattttgc caagtatttg caagaagatg ccaagccaca aacagccaag gttgccacat
    14041 tgttgcaaga ttacggcttc acattgccaa cggtggcgga ttggcaagcc ctagtagcag
    14101 atatcaagca gtttggtcta gccaatgcgc acttgctagc ggttgctccg acaggctcca
    14161 tcagctactt atcatcctgc actccgagtc tacaaccagt cgttgctcct gttgaggtcc
    14221 gaaaagaagg tagcctgggc aggatttacg tccctgccta tcagattgac caggctaact
    14281 atgcttacta tgaacgtggt gcctatgagg tcggcccaaa agccatcatc gacgttgtag
    14341 ctgctgccca aaaacacgtt gaccaagcca tctcattgac cctgtttatg accgatcaag
    14401 cgaccacacg tgaccttaac cgctcctata tccaagcttt taagcaaaac tgtgcatcga
    14461 tctattatgt gcgagtgagg caagacgtcc tagcaggcag cgaacagtat gacgaggata
    14521 gtcttgtgac agctcctgga gctagtgatg agaccaccac agagtgtcaa agctgtatga
    14581 tctaacagct actagaaagc tctcgtgtcc cctatcacat gcataggatc agtgcactcg
    14641 acctttcaag acaagcaagc atcagctctt gcttgtcttt ttttggcctt aaagcccgtt
    14701 agtctgctgc tatgcgaggc tttttttgag cattagaacg tcaaaaaaaa ggacatggag
    14761 tccttttttg gtgatcggtg ttgaggccgt cacaaactgc ccttgaaata cgcttctatg
    14821 tggagctttt tttggtcttg tgacacgtaa gctccaacga cctccagctg acagcctctt
    14881 ggaaacaaga gctcaacctc tgaaggcaca gccgaataag gctcgacaaa ggctgccttg
    14941 gccccttttt tgacacagat gcgcacctcc accggtcgat gggtcatggc gccgtttttc
    15001 aaggctgtcg tgctcataaa actgtgcttg gtgtagcgtg tgccaagctt gatcttacaa
    15061 aggatatgag ggtcaaactg atggttacgg tagtaagacg tgagatcagc gtgtgaaaca
    15121 ccgatatcac gcaaaaacgt ctcgtacaca tagcggtata ccacaatgtt ccacgggatg
    15181 actagccggt gagtcgcagc atcgagctgg gccacctgat cccttagctc aggcgtgagt
    15241 tggctgagct cacctttggt cttgtctagg ctggtgttga taggggctgc ctgctggcag
    15301 gtataagcag ccatcgcctc ttgctcagcc cttgggtaga cgagaccctt gccccagcgc
    15361 tttttaatca gtgtctcagc gcccttttga tccgaaaagg taaaataagc aggcgtcccc
    15421 tcacaaaccg cccgctcata gtgctcacag acaaaaagct cgaccaagcc ctcagtttgc
    15481 cagactagac taaaacagct aagaagaagg atcatagcca gttgatagcg cttttttagc
    15541 atagttcccc ctgacaccag cccaaaaggc tgacccttcc cctaggcctt ttggctagct
    15601 catgatacca ccagtttctc agacaggcta atctttttgt catctctttc tccctaaaca
    15661 gactctctta tcatgatatt atgctattat gagcgatttg tcaatccata gcaaccgttt
    15721 tttagccaga taagtaagct tttgacctag aggcagacaa gagtggcagt acctgccgtt
    15781 tgctgtggtg gctaataagg actaacataa aatatcaaac gctttcaaaa acagctccta
    15841 agcttaaaaa agctgatgaa caattttttg agtttaagag agattgccat gattttctaa
    15901 tcatttgatg tttaaaatga taaaaacatg aacttgaaat gaaagaatac gagtagtaaa
    15961 ctaaaaatat atcaaaaata atgaaatgga gtgattttat gaaaacaaaa tcaaaacgct
    16021 ttttaaacct agcaaccctt tgcttggccc tactaggaac aactttgcta atgacacgtc
    16081 ccgtaaaggc ggaggtgtca gaaattgggc acgatcacgt aacaatcagt tctaatggtc
    16141 aaacagatga gggtgctgca tatggtagag gccatgatga tggaagtaag tttggatatg
    16201 aagcaggatt acaatcgagt tggaatgagt ctgaaccccc atcatcagat aaaatacctg
    16261 agcctagtgt gaatccatat gaaagtagta atgaacaaga tagagaggat tataaagaag
    16321 gttttcgtga tggatatcct gggggctatg tcgcaggctg gagaaaaact catccaatag
    16381 aagctactct tcaatattta tggtatacgg tatctagttg gtttgaaagc ttatttaata
    16441 atagtaagta gatatttgtt tgttgattaa agttacttaa ggtaaaataa aatgtctgtc
    16501 agcaacttcg tgctagtggc ttttgtttta cctttcccac actaatctgc tataattggg
    16561 ataatccctt tagtagtcag gccacctctg ataggggtgg ccttttttgg gttttagtga
    16621 cccgtgtgtt gaaaaaggag catttatgcc agaaaagaaa gtgttgcccg tcctaagcat
    16681 ggtggtgagt tttgtctgcc tgcttgatca tatcttcttt tttgggctct ttgtcaactg
    16741 tagtgggtga cttatagcta aacacgtgag gagacgagac tcgtcttctc ttttgttatg
    16801 ttcaaagcga ggtaaatgcg ttttttaaag ttatcaaagt tccgaaagcc aaaagcattg
    16861 cgtttgatgt gtttgatgag tttattggtt gcttctagtt tggcgtttga acaaggaagc
    16921 tcaatgacat tagtgacata gtttttgtat cgcatcaggg tgttcaaagc cgttttaaag
    16981 atgttattga gcagaagtaa caccttttga attaagtcaa aaaatgctca gtattcttct
    17041 cttgtaagtg aaaaaggagt aactgataaa ggtcatagta atcttttaat tcaggaacgg
    17101 gattaaatag tttattgaga cattgtttag gagttagcgt ttccctaaag gttctagagt
    17161 agaaggcgtt gagcgagagt tttcggctac cttttgaatg agttgacagt agtatttaag
    17221 aactctgtat tccagagact ttttgtcata ttgcttcatg atgtgaatcc gggtttgact
    17281 gagcgctctg ttcatgtgct gaacaatagg aaacgatcta ggacgatgtt agcttgagga
    17341 aatagctttt taatgagcga aatatagctt cctgacatat caacggtaac gattttgaca
    17401 ctgtttctag cctctttaga gtatctaaag aagtgatttc gaatggttgt ttgtcgccgg
    17461 ttgtccagta tggagatgat ttttttggac tcaaaatctt gagcgataaa ggcgagttcg
    17521 cccttttgat aggaaaatcc atcccaagac aacacctcgg gtagtttaga gaagtcttcc
    17581 ttgattacaa actggttgag ttttcggtag acggtagaag atgatatagc cgactgttga
    17641 gcgatgttag tgagagctcc tcgattaacc aaggtttgtg cgactttttg tctgaggggc
    17701 tcagagatct gacagttttt ttgaaccaga cttgtttccg ctacagccac ttttcgacag
    17761 gatttgcatt gagatcttct cttttttaaa cgaatgagac taggaaaacc accaatttcg
    17821 ataaagggga ttttagaatg tttctgggag tcgtatttaa tctgaactcc tttacagtgt
    17881 ttacacttag gtagggatag tcaagtgtgg cgaagacttc gataagtgtc tcgcgttgaa
    17941 aaaccttgtt caaggtgatg tgtttatctt taattctaat caattctgta gtatgataga
    18001 gttgtctcat atgattcttt ctaatgatgg tttggtcgct ttgcattata agtcatatgg
    18061 gattttttgc tacttatagt tacctctact tgccgtttgt agcaagtagt ctagcattgt
    18121 aggtcttgta taaaacaaaa gcaagtgttc gctggctggg cttgtagtag cagtaacagt
    18181 ataatatgaa ccgacagcta ttccctatta aaatagacgc aaatgtccac gctttcactg
    18241 tagttgctag taaggtctaa cacaaaacat caaaggcttt caaaaaaact cctaagcgca
    18301 aaaaggcgga ctaacagccc caagcaagca ctttctgagg taataagaga ttgccatgat
    18361 tttctaatca tttgatgttt aaaatgataa aaaaatgaac ttgaaatgaa agagtgcgag
    18421 tagtaaacta tgaccgtatc aaaaataatg aaatggagtg attttatgaa aacaaaatca
    18481 aaacgctttt taaatctagc aaccctttgc ttggccctac taggaacgac tttgctaatg
    18541 ggacaaccca taaaggcgga ggcagtagta tctgatttga atgatgcagt ctcttcgatg
    18601 ggagatacag cagaggaaac tgacgtcaat atcgatggtc aagaagacga agaagacgaa
    18661 gaagacgatg cttatatgca agggagaaga ctgggagaga aagaggggta tgaagctggt
    18721 caaaaagaca acgctcccag attatttgac agtagtacgc tagagccccc tactaatcct
    18781 tacgaggatg cgggtgatac attgaatagt aattggttta aaagtggtta cagcgatgct
    18841 tatggtcaag catatgagca gggatggaat gatactcatc cgatacaatc aactttaatt
    18901 tggctgtggg ggatagtaag gaacttattt agattttaga tttaggtgac ggttaccaac
    18961 aacaaagtaa ttaatattac gttataaaaa gctagtatcg cttttgagga tagtgttatt
    19021 tactcttttg agatatttga aatactataa cactaaaatg aatcctttta tcacaagcat
    19081 ttcccccact aatctgctat aattgggata gtccctttag tagtcaggcc acctctgata
    19141 ggggtggctt ttttgggttt tagtgacccg tgtgttgaga aaggagcatt tatgccagaa
    19201 aagaaagtgt tgcccgtctt aagcatggtg gtgagttttg tctgcctgct tgatcatatc
    19261 ttcttttttg ctacttatag ttatctctac ttgccgcttg tagcaagtag tctagctctt
    19321 atcagtctgg tgcaaaacaa aggcaggcaa aaggtttggt cgctagctgg gcttatctta
    19381 gctgctctag ccgtggcagt agtggcctat gcctactatg acccaaccgt catcccctat
    19441 taaccttatc tcctctttgc acgcaatcag cagcacaaaa agcaccctta caggtgctta
    19501 gattgaaaaa ggtcttttgt gatacttttt ctgaggcggt gcggacggta gcgacttcct
    19561 ttggaattcc ctgacttatt tttgagacct aaggtctcaa aaatcccgag tggcgtcctc
    19621 tcgtattgac gccactttca ccatgacaaa aatcatctgt tttggctccc tttgtgtgta
    19681 aaaatcggaa accgtatgtc acatcagcag agctgtttat gctttttgaa catagaacta
    19741 ggtttgtcta ggttctaagc acctgtaagg gtgctttttg tactttagcc aacctcctca
    19801 tagcctagca aaccctcact gaaacagtcc cttaaagttc caccaagcgt cttgaaaaac
    19861 atcgcgtact ttatcccgcc atgttttttg ttcctctctt agacgagaat tttcaaaaag
    19921 gttttcggca tattcttcat caaatgcttt atattgttca tttttgagtg tttctgaatc
    19981 aataaaagtc atcattccag ctaaaaatcc atcatctcct tgaaaataaa tagtaaattc
    20041 ttttggaggt tgcgacgttg agatatcgag agtgactgta gtagtcactg aattgctgtt
    20101 tcgttgtttc gtgtttttat caatccatgc agaccatcct ggaggaagtg taacagtgac
    20161 tttattacct gtaatgtgaa caggaatttc atttctgtta tcatctaaaa ctttttgttg
    20221 tcttggaaga ggtacccaag aactctcctc cgctctgacg ctactagtta ccccgatggt
    20281 aaagagtgcc agtagcaaag ttaatgtctt tttcattttt tcctctcctt ttgctcattt
    20341 tttaagacaa gggttattat accaattctt gcgctaaaaa gatagggagt tcatgtcttg
    20401 tggttgggaa atggcgcgac tattcttggt aggatcagct agtttttttc ctagtcagtc
    20461 gctagctgtt gccattttac ctttaaaggt cgtttgggta ttatgtcata ttttatgagc
    20521 ggttttatat ttggtatcaa tatcttatag ggcaattgct catatttatt gaaagctctt
    20581 atcttattta gtccaaagat aaatatcaaa atggctgaaa tctttcatat ttatagtctt
    20641 attgtcttta tattttttaa aaatatcact ccttgtacta gatgaggttg catcgtataa
    20701 gttaaaactt tcatgtttat tgccattgat cgcaatttct aattgccctt ttatgtatgg
    20761 agaattagga tcataaattt tgtatgtttg cataagatat tgtctgattt tgaagtcaaa
    20821 ttcttgaatc gtaaaaatac gtttatctat ttttatggga tttattagtg ttttttgctg
    20881 gactccatct attagtaaat tacctacaat tttactattt tccgaattac tgtttactga
    20941 tggtgtaacc ccaccatata tataataaac atcataacga gtaaatatgt acggaagacc
    21001 aaaaatattt actttttgat ttttcgtaaa ctttgttgta atatagctct cgacctcaga
    21061 atcaactgaa aaatttttct ttttataact ggaaacatca atataaaaat catgagtggt
    21121 caagtaatca atattacaat tcgtataatc tactggtctg atttcgtatg cgtaatttaa
    21181 ttgtagctta acgtctttaa tcttttcact atctgatttt acacttccgt acccatgaaa
    21241 gattattata actaaaatta ttgtttttat taatcttttc atacctctcc tcgctcaccc
    21301 cgtgccccac aaactacttt atcctaaaaa acaataaaat caacattgag gacttttttg
    21361 gttcccataa ttgttaacta tgtcatttta gagcaatcat atagagacta agatataatt
    21421 ttaccaaagt tttgaaggct attgaagagt gactttctcc cttaatttat ttaattaaag
    21481 atactaaaat tcttgatatt cataaagctc aatcaggaag gacaaaatca aagagcttac
    21541 cagttttctg gtaatgctga tcatgtctcc tttgatatac ctgttagcta cagctttttt
    21601 caattcgtga tatgttaaca tttttatcct cctacttaat ctattcgtaa aaaatctctt
    21661 aaaatagaca tttaaaaaaa agtccattat aacggatatg cccgtgtgcg aatacatgtt
    21721 cgggtaaata ttttaaatca agatgtttga tgtttgtgtg tgcaaaaaca aaaaaatcgc
    21781 cctcgattga tgatatgata cgtcttaagt agacaggcta aataaccaaa aatctactca
    21841 agaggtattt ttatgccaga aaagaaaggg ctgctcgtct taagcatggt agtgagcctt
    21901 gtgtccctgc ttgatcatat cttctttttt gctacttata gttatcttta cttgccactt
    21961 gtagcaagta gtctagctat tattagtctg gtgcaaaaca aaggaaggca aaaggtttgg
    22021 tcgatagctg ctttagccgt agcagtagtg acctataccc aacggttatt ccttatcaaa
    22081 tgcaaagtcc ctccctctta tataaactat caaatacttt caaaaacagt tcctaaagct
    22141 caaaaaggtt atctaactgc tccaagcagg cactttctgt gataaggata gcttgccatt
    22201 tttttctaat catttgatat ttaaaatgat aaaaaaatga acttgaaaat gaaagaatga
    22261 gaggtgtaaa ctaaaagtac caaataaaca atgaatacgg agtgatttta tgaaaacaag
    22321 accaaagcgc tttttaaatc tagcaaccct tagcttggcc cttttgggca caaccttact
    22381 aatggcacaa ccggtaaagg cggataatga tactagggag tctcaagagc gcgtagacca
    22441 aagaatagaa gatccttatg atgatggaaa aaatgctggg agaaaggatg gttatgaatc
    22501 agggaatcat aagggaatct ctgaagagcc tccaactaaa actcctgaac ctccgactaa
    22561 tccttatagc cgtgataggg tgaaaagcaa gaagtataga gatggttatc aggaatggta
    22621 tgtctatgga tactatgaag gttggcatag agcaaatgat gaggataaaa ataaagagtc
    22681 ttctgaccat tctcgagatc aggatttaga aggtacagaa cagacagata ctgaatattc
    22741 agcaattatc agtacattgt atggaattgc tggagttgta ttggaatatg tgctatcttg
    22801 gtttagtatc taagatttag ttttggttac tggataatgg tttaaatatt tattattatc
    22861 taaaaatggc gtgatcaaat tttgatgatt tggttaatat tgtttagaac agactgaaga
    22921 caaagctctt agtaactaaa ttctaagggc tttgtcttca gtctgaggca ccttacgggg
    22981 tgtcttttat tatttaggtg gcttaatgaa ttggttaaaa tctttttagt ttagtaaaat
    23041 tgctatttaa ggttagccac ctctcgtatg gtcacagttg atgaattatg ctatagtata
    23101 gacataaaaa atagttcaaa tcccttcttg cctgttggtg gattttgaac tgaaagcctc
    23161 tctgattgca gttggtaggc ttttttgatg ctttttcgta ctttttttat ttattacgct
    23221 aaaaaatgtt atacttgatg aagaaaaagg agagtttaga acgttaagat acctcacagc
    23281 agattgatag aggatatgga aaaaggttag ctttcccaag agatgccgtt tgggaatggt
    23341 ataacaataa ctggcagaag ttataagtaa aacaagaaca aattaactca gatgattttt
    23401 agacgttccc acgcgtgtgc gaattaattg gaaaaaaatg agaaaatacg atatacaaaa
    23461 aaggagaaca atatgacatt tgaagaaatt ttaccaggtt taaaagccaa gaaaaaatat
    23521 gtacgtacag gttggggtgg cgctgaaaat tatgtgcagt tgtttgatac ccttgaagtg
    23581 gatgggaaag tactacaggc gacgccttat tttctgattc atgttactgg tgaaggtgaa
    23641 ggtttttcca tgtgggcacc gacaccatgt gatgtgttag cagaggattg gatcgaggtc
    23701 aatgactaaa gttgttttag tgacgggctg tgctagtggg attggttatg cacaggcccg
    23761 ctattttttg agacaagggt atcacgtata tggtgtggat aagtcggata agccggactt
    23821 aagcggtaac tttcacttta tcaaattgga tttatcctct gagttatccc cactctttaa
    23881 agtggtcccc agtgttgata ttttgtgtaa tactgcaggg attttagatg cctacaagcc
    23941 acttttagac gttagtgatg aagaagtgga acacctattt gatattaatt tttttgcgac
    24001 ggtgaagtta acacgtcatt atcttagacg tatggtggaa aagcagtcag gtgttatcat
    24061 caatatgtgc tcaatagcaa gttttatagc gggtggtggt ggtgttgcct atacgagttc
    24121 taagcatgcg cttgcgggct tcacgcgtca attggctttg gattacgcca aagatcagat
    24181 tcacatcttt ggtatagcgc caggtgctgt caaaaccgct atgacggcta gcgactttga
    24241 gcctggtggc ttagcagaat gggtagctcg tgagacgcca attgggcgtt ggactgaacc
    24301 agatgaagtt gctgaattaa cgggatttct tgcgtcagga aaagcgagat caatgcaagg
    24361 tgaaattgtc aaaattgacg gtggctggac tttgaaatga tcacatgcct tagcttgagc
    24421 tggcgcagta agccctagag gtgtgatgag tcaaacagtc aaaggagatg aacaaatgaa
    24481 attagcggtt ttggggacag gtatgattgt taaggaagtt ttgcctgtct tgcagaaaat
    24541 tgatggtatt gatctagttg ctatcttatc aacagttaga agtttaaaga cagctaaaga
    24601 cctcgctaag gcatatcaca tgcctctagc tacgagtgaa tatgaggctg tcttaggcaa
    24661 tgaggagatt gacacggtct atattggact gccaaatcac ctgcattttg cctatgctaa
    24721 ggaggctctt ctggctggta aacatgtcat ttgtgaaaag ccctttacca tgacagcagg
    24781 agaattagat gaactggtgg taatcgctaa aaaacgaaag cttattttgt tagaagcgat
    24841 tacgaatcag tatttaaaca acatgacctt tatcaaagag catcttgacc aacttggcga
    24901 tatcaaaatt gtagaatgca attattcaca atattcttca cgctacgatg tcttcaaacg
    24961 aggtgatatc gcgccagctt ttaatcctaa aatgggtggt ggagctttac gtgatttaaa
    25021 catttacaac attcattttg tggtaggatt atttggtcgc cctaaaacgg ttcaatattt
    25081 agccaatgtc gaaaaaggca ttgacacatc aggcatgctt gtcatggact atgagcagtt
    25141 taaagtggtg tgtatcgggg ccaaagactg cactgcggaa atcaaaacaa ccatccaagg
    25201 taataaaggg tcactggcag tgcttggagc aacaaatacc cttcctcagg ttcagttaag
    25261 tctacacggt cacgaagcac aagtggttaa tcacaacaag tatgaccacc ggatgtatga
    25321 agagtttgtc gcttttaggg acatgattga ccaaagagac tttgagaaag tgaatcaggc
    25381 tttggagcat agccgagctg tcatggctgt gttggagcga gctgttcatt cgtgatggta
    25441 atctggtagc cattgccttt tgtgcagttg ctatggtaat ggttgaaaga cgtgttttca
    25501 cgagggttaa ccccctcgtg cttttttggt aaaaataact tgttcaggat ttgggccttc
    25561 tttgtgagat gagagatgcc tgacgaattg atttgattaa aataatgata aagccaaacg
    25621 atttaagaaa tgaccgttcg gaaattctgc cccaaaaggt ctctagaagc agtaaaatcg
    25681 aatttgtaag cgttgacact gctgcataac tctgttacac taagagaatg tcatgacaaa
    25741 aattacagtg taggagttta attgtttatg gaagaggatt ataacaaaag agaacctgag
    25801 aagtttacgc aatttctgcg ccgtcaaaag gtcgtctttt ttgtcgcatt ttttggttat
    25861 gtgtgtgctt acttggtacg caacaatttc aaactaatgt caaacactat tatggtccaa
    25921 aacggttggg acaaggctca aattgcgacc ttgttatcgt gtttgaccgt ttcttatggc
    25981 ttagcaaaat tctatatggg ggccttaggg gatcgtgtta gcttgagaaa gctcttttcg
    26041 atcagcttgg gtgcaagcgc tttgatttgt attttaattg gttttttcaa tagttcaatg
    26101 gtagtgttag ggatactgct agtcttgtgt ggtgttgtac aaggtgcctt ggcacctgcc
    26161 tcacaagcca tgattgccaa ttattttcca aataaaacac gcggcggagc catcgctgga
    26221 tggaacattt ctcaaaacat gggatcagcc ctcttgccat taaccatcgc cttgctcacc
    26281 agtatgggct tggtggtacc agctaatggc aatattttac ttgccttttt aatccctggt
    26341 gttttagtgt tcttgtttgc tttgtgttgc tggaagcttg gcggtgataa tcctgagtct
    26401 gagggacttg actctcttcg aacgatgtat ggtgatgcgg gcgaatcagc tgttgccagt
    26461 gaagaagaaa agcataacct gtcttattgg caactcatct ggaaatacgt tttttgtaac
    26521 ccgtcgctct tacttgttgc tgctgtcaat gtggcccttt attttgttcg ttttgggatt
    26581 gaagactgga tgccgatcta cttgtcacaa gtagccaata tgtcagaggc ccatattcat
    26641 tttgcgattt caatgttaga gtgggtagct attccaggct cgctggtatt tgcgtggtta
    26701 gcggttcgtt atcctaataa aatggccaag attggggcta ttgggctttt tgtgttagcg
    26761 gctattgtgt ttgtctatga acgcttgact gccacaggtg ctccaaacta tttcttgttg
    26821 cttgttattg caggtatttt agggtcattg atttatggcc cacagttgat cgtgaatatt
    26881 ttaacaatca actttgttcc tttaaacgtt gcaggaacag cgattggttt tgtaggagta
    26941 acagcttatc tcatcggcaa tatgggagca aactggctga tgccgatttt ggcagatggt
    27001 tttggctggt tttggtcata tattgtcgtt gcagccttat ctgctttttc agcggttggt
    27061 tatttaattt tagccaaacg tgaggaagaa atcatcaaag attagctagg caaatgccta
    27121 agaaagacag acttattgct gaagtcctgt cttttttagt catcacaagc cgcaagtaac
    27181 actataaaag acaagaaaat gctagggtgt ttcaggaaaa aaagagaaaa tatgctataa
    27241 tgagaaggta tttaaattat atagtaagga aaaacaaatg acaaattatg ccattatttt
    27301 agcagcgggc aaagggactc gcatgacttc tgatctccca aaagttcttc ataaagtatc
    27361 tggactaacc atgcttgagc atgtttttcg tagtgtaaaa gcgattagtc ctgaaaaatc
    27421 cgtgacggtt attggtcaca aatcagaaat ggtacgtgct gtcttagcag atcaatcagc
    27481 ctttgtccat caaacagagc agttaggaac tggccatgcc gtgatgatgg cagaaacaca
    27541 actagaagga ctagaagggc ataccttggt gattgcagga gatactccct tgatcactgg
    27601 agaaagcctg aagagtctga ttgattttca tgtgaaccat aaaaatgtcg ccactatttt
    27661 aacagctacg gctcaagatc catttggtta tggtcgtatt gttcgcaaca aagatggcga
    27721 agtgatcaag attgttgagc aaaaagacgc caatgagtat gagcaacaat taaaagaaat
    27781 caatacagga acttatgtgt ttgataacaa gcgtctcttt gaagcgctca aatgcatcac
    27841 taccaacaat gctcaaggag agtattacct gactgatgtt gtgactattt ttagagcaaa
    27901 caaagaaaag gtaggagcct atatcctgag agacttcaat gaaagtcttg gtgttaatga
    27961 tcgtgtggcc ttagcaacag ctgaaacagt gatgcgccag cgcattaccc aaaaacatat
    28021 ggtcaatggg gtgactttcc aaaatcctga aaccgtttat attgaaagcg atgttgagat
    28081 tgctccagat gtccttatcg aaggaaatgt gaccttgaaa gggcgtacac atatcggctc
    28141 aggaactgtc ttgacgaatg gtacttatat tgtggattct gaaattggtg ataactgtgt
    28201 tgttactaat tcaatgatcg aatcatctgt tttagctgca ggagttacgg ttggccctta
    28261 cgcccacctt cgaccaggaa cgaccttaga tagggaagtc catatcggta actttgttga
    28321 ggtcaagggt tctcacattg gtgaaaaaac aaaggctggg catctgactt atattggaaa
    28381 cgctcaggtt gggtcttcag tcaatgttgg ggctggaacc atcactgtca actacgacgg
    28441 ccaaaacaaa tatgaaacag tcattggaga tcacgctttt attgggagca actcgactct
    28501 cattgcacct ttggaagttg gggatcacgc tttaacagca gcaggttcaa cgatttcaaa
    28561 aacggttcct atcgatagca ttgctattgg tcgtagccgc caagtcacaa aagaaggtta
    28621 tgccaagcgc ctagcgcatc acccaagtcg aagcaaataa ttcaagatct atttagtaag
    28681 gaggccaggc atgaaatttg aagaaaaaac gcttaagaga caaacagttt ttgatggtca
    28741 tatttttaaa gttgttgtgg atgatgttga acttcccaac aatctggggc aatccaagcg
    28801 agaattaatt tttcatcgtg gtgccgttgc tgttttggct attactcctg aacgtaagat
    28861 tctccttgtc aaacaatacc gtaaggctat tgaacgtgtc tcatacgaga tcccagcggg
    28921 taagctagag attggtgagg aaggctcaaa gctaaaagct gctgcacgtg agctagaaga
    28981 agaaacagcc tatacgggga cattgacgtt tttgtatgaa ttttatacag cgattggttt
    29041 ttgcaacgaa aaaatcacac ttttcttagc gacagaccta acacaagttg ccaatcccaa
    29101 acctcaagac gacgatgaag ttatcgaggt cttagagttg acatatcaag agtgcatgga
    29161 cttagtcgct caaggtaaac ttgctgacgc taaaacgttg attgctttgc aatactatgc
    29221 cttacatttt ggaggtgagc agtgatggga aggccattgt tgaccgatga tatcatcgaa
    29281 aaagcgagac gaatggaaac atttgagtca gatgatgctg tagactttga cacaaaagta
    29341 atgacgctac cagaaaaaga tgataaagca cgcatttata aaagccgtcg gattgaaaat
    29401 gccaa
//
LOCUS       DAUVUY010000011        27364 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.011, whole genome shotgun sequence.
ACCESSION   DAUVUY010000011 DAUVUY010000000
VERSION     DAUVUY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27364
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.011"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..619
                     /locus_tag="VOX58_000487"
     CDS             <1..619
                     /locus_tag="VOX58_000487"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HES5877743.1"
                     /translation="DTVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPL
                     DTITEEQFEKAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYG
                     GTKFAVRGITQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQ
                     FAKDITLKRLSEPEDVANAVGFLVGDDSNYITGQTIVVDGGMVFH"
     gene            727..3228
                     /locus_tag="VOX58_000488"
     CDS             727..3228
                     /locus_tag="VOX58_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HES5877744.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLSHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLAYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRVQDDKDFTR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRQDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            3563..4756
                     /locus_tag="VOX58_000489"
     CDS             3563..4756
                     /locus_tag="VOX58_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HES5877745.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            4786..6132
                     /gene="asnS"
                     /locus_tag="VOX58_000490"
     CDS             4786..6132
                     /gene="asnS"
                     /locus_tag="VOX58_000490"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HES5877746.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            6547..7437
                     /gene="rapZ"
                     /locus_tag="VOX58_000491"
     CDS             6547..7437
                     /gene="rapZ"
                     /locus_tag="VOX58_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HES5877747.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            7434..8411
                     /locus_tag="VOX58_000492"
     CDS             7434..8411
                     /locus_tag="VOX58_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HES5877748.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLTPPGDLRNVLLAMSDMPKFYERVFQYRFNESDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            8408..9319
                     /gene="whiA"
                     /locus_tag="VOX58_000493"
     CDS             8408..9319
                     /gene="whiA"
                     /locus_tag="VOX58_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HES5877749.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYVQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            9452..10849
                     /locus_tag="VOX58_000494"
     CDS             9452..10849
                     /locus_tag="VOX58_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HES5877750.1"
                     /translation="MACTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMIVVKPEDQ
                     PRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATETITTNSR
                     VLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVAFSDEHDI
                     WWLETIGGHHWIARRVPDDAYVTNPNQFGIDYFEFNNPEDYLCSADLKDFIDTYHLDL
                     TYNHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRSFALAWCQKPYRKI
                     TVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRPNKPQEIA
                     AIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAALADPHYNH
                     HETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQTLLNKIL
                     FDASNLMTNRFSLSD"
     gene            11001..12548
                     /locus_tag="VOX58_000495"
     CDS             11001..12548
                     /locus_tag="VOX58_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HES5877751.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADVFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGVGHDHHHEEADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            12696..13418
                     /locus_tag="VOX58_000496"
     CDS             12696..13418
                     /locus_tag="VOX58_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HES5877752.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMTENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKVGKTPKTEILS
                     FEQYQVTPYLSGLLEFDLDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEADLLGIKKGDPVLHIIRKTYNDKNILI
                     EFTFSIARADQFRYRVQHHPNG"
     gene            13438..14637
                     /locus_tag="VOX58_000497"
     CDS             13438..14637
                     /locus_tag="VOX58_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HES5877753.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCKSANGNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQCYLFESVASTDLVAAP
                     DYYLAEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(14734..14994)
                     /locus_tag="VOX58_000498"
     CDS             complement(14734..14994)
                     /locus_tag="VOX58_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HES5877754.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(15109..16050)
                     /locus_tag="VOX58_000499"
     CDS             complement(15109..16050)
                     /locus_tag="VOX58_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HES5877755.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLTWISQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            16444..16893
                     /locus_tag="VOX58_000500"
     CDS             16444..16893
                     /locus_tag="VOX58_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HES5877756.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSDQFALTGAVKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            17068..17352
                     /locus_tag="VOX58_000501"
     CDS             17068..17352
                     /locus_tag="VOX58_000501"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HES5877757.1"
                     /translation="MRLEEIRQEINGIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQRLTGGDIND"
     gene            17345..18607
                     /locus_tag="VOX58_000502"
     CDS             17345..18607
                     /locus_tag="VOX58_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HES5877758.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGATVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFAYLLGWFKPYLSQKLPNPYFRIAFIGALLSICLMIGHV
                     GRYSGLGTHLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            18722..19069
                     /gene="rplS"
                     /locus_tag="VOX58_000503"
     CDS             18722..19069
                     /gene="rplS"
                     /locus_tag="VOX58_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HES5877759.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            19316..19387
                     /locus_tag="VOX58_000504"
     tRNA            19316..19387
                     /locus_tag="VOX58_000504"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19349..19351,aa:Arg,seq:cct)
     gene            complement(19430..19687)
                     /locus_tag="VOX58_000505"
                     /pseudo
     CDS             complement(19430..19687)
                     /locus_tag="VOX58_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            20083..20652
                     /locus_tag="VOX58_000506"
     CDS             20083..20652
                     /locus_tag="VOX58_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HES5877760.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVAMFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            20653..22605
                     /gene="gyrB"
                     /locus_tag="VOX58_000507"
     CDS             20653..22605
                     /gene="gyrB"
                     /locus_tag="VOX58_000507"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HES5877761.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYNVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRNFIEENAVYSTLDI"
     gene            22973..24697
                     /gene="ezrA"
                     /locus_tag="VOX58_000508"
     CDS             22973..24697
                     /gene="ezrA"
                     /locus_tag="VOX58_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HES5877762.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(24829..25287)
                     /locus_tag="VOX58_000509"
     CDS             complement(24829..25287)
                     /locus_tag="VOX58_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HES5877763.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQPPEKTKSFWKRFLKK"
     gene            25514..26821
                     /gene="eno"
                     /locus_tag="VOX58_000510"
     CDS             25514..26821
                     /gene="eno"
                     /locus_tag="VOX58_000510"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HES5877764.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
BASE COUNT         8439 a         4833 c         5743 g         8349 t
ORIGIN      
        1 ggatactgtt gcagttgtag cagatgtttc aaaacgtgac caagtttttg atgctttcca
       61 aaaagttgta gatacttttg gtgatttgaa tgttgttgtt aacaatgcag gtgttgcccc
      121 aacaacccct cttgacacga ttactgaaga gcagtttgaa aaggcttttg caatcaatgt
      181 cggaggaacg atttggggaa gccaagcagc tcaaaaacat tttagagaat taggtcatgg
      241 cggaaaaatt ataaatgcaa cgtcacaagc aggatgtgaa gggaatccta atttgactgt
      301 ttacggtggt acaaaatttg cagttcgtgg tattacacag actttggcaa aggatttggc
      361 atcagaagga atcacagtca atgcttacgc accaggtatt gttaaaacac ctatgatgtt
      421 tgatattgcc catgaagttg gtaaaaatgc aggcaaagat gatgaatggg gtatggagca
      481 atttgcaaaa gatatcactt tgaaacgctt gtctgaacca gaagatgttg ctaatgcagt
      541 aggcttctta gtgggagatg attcaaatta tatcactgga caaacaatag ttgtcgatgg
      601 tggtatggta ttccattaaa aatgattaat actgacactg tgagtttcac tcacagtgtt
      661 ttttatattt cttaaaaaat tatcctatct tgattattgg agtgatggca cctttgaggt
      721 atagtcatgt tttgttttat agatatagcg tgctataata gacttactat gacccagaaa
      781 aaacttagaa aatatgcagt agtggacttg gaggctactg gagcaggtcc caatgcttct
      841 attattcagg ttgggattgt tattatccaa ggaaataaaa ttattgatag ttatgaaaca
      901 gatgttaatc ctcatgaatc tttggatgag catattgttc atttgacagg tattacggat
      961 aagcagttgg ctaaagctcc ggattttggt caagtggcgc atcacattta tcaattgatt
     1021 gaagattgta tttttgttgc ccacaatgta aagttcgatg caaatttatt ggcagaagct
     1081 ctttttttgg aaggatatga gttaacaata ccacgtgttg atactgttga attagcccaa
     1141 ctatttttcc ctcgctttga aaagtataac cttagtcacc tatctaggca gctcaatatt
     1201 gatttggcag aagctcatac tgctattgca gatgcaagag ctacagctat actgttttta
     1261 agacttttac aaaaaattga gagccttcca attgagtgct tagaaagctt attagcctat
     1321 tcagatagcc tgctatttga aacagcaatg gtcattcaag aaggattagc caaggccaaa
     1381 ccttatgatc ccaacaaata cattaaaatc agacagattc tcttaccaaa gggctcaaaa
     1441 gctctcaagc cttatcaaat atcaaaaagc ttccctatta atatggcact attaggtctg
     1501 gaagaaaggc ctaaacagac tcaatttgct cagttaattg acgaagatta ccatcaggga
     1561 gtagctagtt ttattgaagc tcagacaggt attggaaaaa cctacggcta cctgctaccc
     1621 cttttggcaa aagaagatca aaaccaaatc attgttagtg tgccaaccaa acttcttcaa
     1681 gaccaattaa tggctggtga agtagctgct attcaagaac aatttcacat cgcctgccat
     1741 agtttaaaag ggcctgccaa ttacctaaag ctagatagct ttgcagatag tttggaccaa
     1801 aatgaccaga accgtttggt gaatcgttat aaaatgcaat tattggtttg gctcttagaa
     1861 actaagacag gagaccttga cgaaatcaaa caaaagcagc gctttgccgc ttattttgaa
     1921 cagcttaagc acgatggaga cattaagcag tcttctgaat tttacgatta tgatttttgg
     1981 agagttagtt atgaaaaagc caaaacagcc cgtctcctga ttaccaacca tgcttatttt
     2041 ttacaccgtg ttcaagatga caaggacttt actagaaaca aggtcttggt atttgatgag
     2101 gcccaaaagc tgatgctcca attagatcaa ttatcacgtc atcagcttaa cctgacggtt
     2161 tttttacaaa caatacaggc caagttaagc aaccctttac ctcttttaga aaaacgctta
     2221 ttggaaagct tgtcgtttga actgggacaa gtgtcaagtg attactatca aaataaagag
     2281 caccagcttg ctcatgattg gtctcgtata gctggctacg ccaaagaatt aacaggagca
     2341 gattatcaag agttacaagc cttttttgct acctcagatg gtgactactg gcttagtagc
     2401 gaaaagcaag aggagaagcg agtgacttat ctaaacagtg ctagtaaggc atttattcat
     2461 ttccaacagc tcttaccaga aacggtgaaa acttattttg tttctgccac tttaaccatc
     2521 agctcagaag taaccttggc agacctatta ggttttgaag agtatctcta tcatgtcatt
     2581 gaaaaggata aaaagcaaga ccagttggtg cttgttgatc aagaggcgcc tatcgtgaca
     2641 gaagtcagtg accaaatcta tgtggaagca attgcaaaac gaatcgaaag cttgaagcaa
     2701 gagggttatc ctattttagt gctttttaat tctaaaaaac acttgctgtt ggtttcagac
     2761 tatctagacc aatggcaagt gcctcactta gcgcaggaaa agaatggaac agcttataat
     2821 attaaaaaac ggtttgatca aggagagcaa actattttgc ttggtttagg ttctttttgg
     2881 gaaggagttg attttattca agcggatcgt atgattaccc ttatcgcacg attgcccttt
     2941 gataacccag aggatttctt tgttaagaaa atgagtcatt atctattgga aaaaggtaaa
     3001 aatcctttca gagattactt cttgccaatg actattttgc gattaaaaca ggctattggt
     3061 cgcacgatgc gtcgacaaga tcaaaagtca gtggttatta ttttggatcg tcgtctcctt
     3121 acaaagtcct atggacaagt tattttggaa ggtctcggtc aggaattttt gatttctcaa
     3181 caaaattttc acgattgtct agtagaaacg gactgttttc tgatataatg gaaaggatct
     3241 attatatcag aaaaattttt aatggcgtta agcacaatca actaacattt aaattagttt
     3301 gaacttttat tatgctcttt tagacaactg ttcaaatgtt ttcgaattgt ttttttgata
     3361 aggacaataa atgaacaaat ttttggtttt agtaaaccca attactgttc ttttcattaa
     3421 tgtcagttgc tatcaaaaca gcttgtcata gttcgtgcga tcagtagcga gtattggtaa
     3481 aactgaaacc gtagattatg aaaaaatgat ctgttgatca ttaattaatc tgaagataag
     3541 aagtcgataa aaaggagata cgatgcccaa actatcaaag cgagttttag agatgaaaga
     3601 gagtgttact ctggctgcag gtgctagagc taaggctttg aaagcgcagg gtcgtgatgt
     3661 actgaattta actttaggag aaccagattt ttttacacct aagcacattc aagacaaggc
     3721 gattgaatct attcaaaatg gtacggctag cttttataca aatgcttctg ggcttccaga
     3781 attaaaagct gcgattgcca catacctgaa aaatcagtat gggtatcact tgtctcctga
     3841 tcagattgtg gcaggaacag gtgccaagtt catcctttat gcctttttta tggctgtttt
     3901 aaatcctggt gaccaagttc tcattccaac accatattgg gtatcttatt ctgaccaagt
     3961 aaaaatggca gaaggtcagc cgatcttcgt tcaaggacta gaagaaaatc aattcaaggt
     4021 aactgttgac cagctggaaa gagcaaggac aagtaagact aaagttgtcc ttatcaattc
     4081 tccttccaat ccaacaggca tgatttatgg tgctgaggaa ttaagggcca ttggagaatg
     4141 ggcggtgcac aacgatattc ttattctggc agatgatatt tacgggtcct tagtctataa
     4201 cggcaatcag tttgttccta tttcaacact ctcagaagcg attcgtcgtc aaaccattac
     4261 ggtcaatggc gtggctaaaa gttatgccat gacaggctgg cgggttggtt ttgcagcagg
     4321 tgaaccagag atcatttcgg caatgagtaa gatcatcggg cagactacct ctaacctgac
     4381 aaccgtttca caatatgctg ctatcgaggc tttctgtggt agtcagtcat cacttgaaga
     4441 gatgcgatta gcatttgaag agcgccttaa tatcacttat ccattgttgt gtcaggtccc
     4501 tgggtttgag gttgttaaac cacaaggagc tttttacttc tttccaaacg ttaagaaagc
     4561 catggaaatg acaggttttt ccgatgtgac aagctttgct aatgctattt tagaggaagt
     4621 cggattagct gttgtgagtg gagcaggctt tggcgcaccg gaaaatgttc gtctgagtta
     4681 tgctacagat atcgaaaccc ttaaagaagc tgttcgtaga ctgcatgtct ttatggaaag
     4741 taatgaaatt aattaaatta attaaataaa aatagagaga aacttatgtc aaaaaaactt
     4801 atttcaattg ttgatgttaa agattatgtt ggtcaagaag ttactattgg tgcttgggtt
     4861 gctaacaaat caggtaaagg taagattgcg tttgtacagt tgcgcgatgg ttcggctttt
     4921 ttccaaggtg tcgcttttaa accaaacttc attgaaaaat atggtgaaga atcaggtctt
     4981 gaaaaatttg acgttattaa acgtctcaac caagaaacat ctgtttatgt aacagggatt
     5041 gttaaagagg atgagcgttc taaatttggc tatgagcttg atattactga ccttgaaatc
     5101 attggagagt cacatgaata tcctataact cctaaagagc atgggacaga cttcttaatg
     5161 gacaaccgtc atctttggtt gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac
     5221 gctattattt atgcaactta tgaattcttt gaccaaaatg gttttatcaa atttgatagc
     5281 ccaattttat cagaaaatgc agcagaagat tcaacagagt tattcgaaac agactatttt
     5341 gggaaaccag catttcttag ccaatctggt caattatacc ttgaagctgg agcaatggct
     5401 ctaggccgag ttttcgactt tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc
     5461 catttaacag aattttggat gatggatgct gagtattctt tcttatcaca tgaggaatca
     5521 cttgaccttc aagaagctta tgttaaagca ttgatccaag gcgttctaga tcgtgcgcct
     5581 caagcacttg atattttaga acgtgatgtt gaggctctca aacgttatat cacagaacca
     5641 ttcaagcgtg tttcttatga tgatgctatt acacttcttc aagagcatga agcagatgaa
     5701 gatacagatt atgaacattt agagcatggt gatgactttg gttccccaca tgaaacttgg
     5761 atttctaatt actttggagt accgacattt gtagtgaatt acccagcaag ctttaaagca
     5821 ttttatatga aaccagttcc tggaaatcca gaacgcgttc tttgtgctga tcttttagct
     5881 ccagaaggtt atggagagat cattggcggc tcaatgcgtg aagacaacta tgatgctctt
     5941 gtggctaaaa tggatgaact aggtatggat aaatcagaat atgactttta ccttgacctt
     6001 cgtaaatatg gctctgttcc tcatggcggt tttggtatag gtattgaacg tatggtaact
     6061 tttgttgctg gtaccaaaca tattcgcgaa gctattccat tcccacgtat gttacatcgg
     6121 attagaccct aataatacaa aaaagttcat gtcatctatg aactttttgt attggtaaga
     6181 ggtgagtgtg ttataaaaaa atcaatatgt cctaaaggag agataagaaa tagtagagtt
     6241 tgaacctaca tcctttgtga agataaattt tggaagttta gtagatcagt tagcagataa
     6301 gacaatatag ccttgctcaa gggatatttt atagacaagt atttggacta cgattatcaa
     6361 atagacagtc tcatttgata ctaaaaaggt ctcgtctatt catataatag gagagttacg
     6421 ggagttatta ttaaaattag ctgattttta cagttcaaaa tgtgttacca ctttttcact
     6481 tcttagcttt cttatgtatg tggtataatg aaggtggacg gttcgataga gaaagggaaa
     6541 ttgtaaatgt cagacaaaca cattaattta gttattgtga caggaatgag cggcgctgga
     6601 aaaacagttg ccattcagtc ttttgaggat ctaggctact ttaccattga taatatgccc
     6661 ccagccttgg ttccaaaatt tttagaatta attgaacaaa ccaatgaaaa tcgtagggtg
     6721 gctttggttg tcgatatgag aagtcgtttg tttttcaagg aaattaattc taccttagat
     6781 agtattgaaa gcaatcctag cattgatttt cggattcttt ttttggatgc aacggatgga
     6841 gaattggtgt cacgctataa agaaaccaga cggagccacc ctttggctgc ggacggtcgt
     6901 gtgcttgatg gtattcgatt ggaaagagaa ctcctatctc ctttgaaaag catgagccaa
     6961 catgtggtgg atacaacaaa attgacccct agacaattgc gtaaaaccat ttcagaccag
     7021 ttttctgaag ggtctaatca agcctctttc cgtattgaag tgatgagctt tgggttcaaa
     7081 tatggtcttc ctttggatgc ggatttggtt tttgatgtgc gttttctacc caatccttat
     7141 tataaggtag agcttcgtga aaaaacagga ctagatgagg acgtttttaa ttatgtgatg
     7201 tctcacccag aatcagaggt gttttacaag catttgttaa accttattgt ccctatctta
     7261 ccggcttacc aaaaagaagg gaagtctgtc ttgacggtgg ctattggctg cacaggaggc
     7321 caacaccgca gcgttgcctt tgcccattgc ttggcagaaa gtctggcaac agattggtcg
     7381 gttaatgaaa gccatcgtga tcaaaatcgt cgtaaggaaa cggtgaatcg ttcatgaaaa
     7441 atcctaaaat gactgttata ggtgggggta ctggcatctc cattatccta aaaagccttc
     7501 gtaatgaagc agttgatatt actgctgtgg tcactgttgc agatgatggt ggttcctcag
     7561 gagaacttcg taatgccatg caattaaccc cgccaggaga cttacggaat gtcttgttag
     7621 cgatgagtga catgccaaaa ttttatgagc gggttttcca ataccgtttt aacgagagtg
     7681 atggagcctt ggctggtcac cctctaggca atctcattat tgctggtatc tctgaaatgc
     7741 aaggctcaac ctacaatgcc attcaaattt taaccaaatt tttccatatt acaggtaaaa
     7801 tttacccctc tagcgagcag gccttgaccc ttcatgctgt ttttaaagat gggcacgaag
     7861 tagcaggaga aagttctatt gccaaatatc aaggcatgat tgaccatgtc tatgtgacca
     7921 acacctacaa cgaccaaaag cctcaggcca gtcgtaaggt ggttgaggct attctagaaa
     7981 gtgacatgat tgtcttggga ccaggctcac tgtttacctc tattttacct aatttggtca
     8041 ttccagaaat taaagaagct ttgcgccaga ctaaagcaga agtggtgtat atttgtaata
     8101 tcatgaccca gtatggtgaa acagaacaat tttcagatgc agaccatgtg gctgttttaa
     8161 atcagcactt aggaagagat ttgattgata cggttttggt aaatgtggct aaggtgccgc
     8221 aggcttacat gaattctaac aaatttgatg aatacctcgt tcaggttgat cacgactttg
     8281 ctggcctttg cagggctgct aagcgtgtga tttcgtctta ttttttacgg ttggagaatg
     8341 gcggagcttt tcacgatggt aacttagtgg tggaagaatt aatgaattta gtgaggatag
     8401 tcaagcaatg agttttacaa ccaaggtcaa agaggaatta attcacttgt ccacaggtga
     8461 caacaatgaa ttggccgcca ttatcaaatt atcaggcagc ttgggcttag ctcaccaaag
     8521 ccttcattta tccattacca ctgaaaatgc taaaattgcc cgctacattt attctcttat
     8581 tgaagacgcc tatgttatcg tgccagaaat caggtatcat caaaagacga acctgcgcaa
     8641 aaaccgtgtt tacaccgtct atgttgagca aggtgtggaa accattttag ccgatttgaa
     8701 attagcggac tccttttttg gtctggaaac aggcatcgaa cctcaggttt tatcagacga
     8761 taatgctggc cgttcttatc ttaaaggggc ttttctagcg gcaggtagca tcagagatcc
     8821 tgagtcaggc aagtatcagt tagaaatcta ttcagtttat cttgatcatg ctcaagattt
     8881 agctcaactc atgcaaaaat ttatgctaga tgccaaaacc atcgagcata aaagcggtgc
     8941 agttacttat gtgcaaaagg cagaagatat catggacttt ttaattataa ttggtgccat
     9001 gtcctgcaaa gaagactttg aagccatcaa attacttcga gaagctcgaa atgatattaa
     9061 ccgtgccaat aatgcagaaa cagctaatat tgccaaaact atcagtgcta gcatgaaaac
     9121 cattaataat attattaaaa tcatggacac aattggttta gaaagtttac cgattgaatt
     9181 gcaacaggta gcccagcttc gggttaagca tccagactat tccattcaac aagttgctga
     9241 tgctcttgag ttcccaatca ccaagagcgg ggtcaaccat cgcttgcgta aaatcaataa
     9301 aattgcagat gacttataga acatttttat cgtatgagcc tttgttcata cgattttttt
     9361 gtctaaaagt cgttttccat tgtttttttg aagtgtctga tgttctgtga tatgataaaa
     9421 gggataataa cgtttgtaaa ggagactgat aatggcatgt acaactatac tcgtcggtaa
     9481 aaaggcatct tatgatggct caaccatggt tgctcgaaca gaagattctc aaaatggtga
     9541 tttcacgcct aaaaaaatga ttgtggtgaa accagaagat caaccgagac attaccgttc
     9601 ggtgcaatct tcctttgaaa tggacttacc ggacaatccc atgacttaca cgtctgttcc
     9661 cgatgcgctt ggtaaagatg gtatctgggc agaagcaggc gtgaacgagg ctaatgtggc
     9721 catgtcagca actgaaacca tcactaccaa ttctcgtgtt ttaggggcag atcctctagt
     9781 agcttctggt attggtgagg aggacatggt gaccttagtg ctcccttata tccgttctgc
     9841 gcgtgaaggg gttttgcgcc ttggtgccat tttagaagac tatggcactt acgagtcaaa
     9901 tggtgtggct ttttcagatg aacatgacat ttggtggctg gaaacgattg gtggtcatca
     9961 ctggattgca agacgggtgc cagatgatgc ttatgtcacc aatcccaatc aatttggcat
    10021 tgattatttt gaatttaaca atcctgaaga ttacctttgc tcagctgatt taaaggactt
    10081 tattgacacg tatcacttag atttaaccta taaccatgag cattttaatc cccgttatgc
    10141 ctttggtagt caacgtgaca aagaccgtca ctataatacg ccacgtgctt ggatcatgca
    10201 aaaattctta aacccagaaa ttgtgcaaga cccgcgcagc tttgcccttg cttggtgtca
    10261 aaaaccttac cgtaaaataa cagtagaaga tgtcaaatat gtattgagta gtcactatca
    10321 ggacacggtt tatgaccctt acggttcaga aggaactcct gttagtaaga aagtctttcg
    10381 ccctattggc attaaccgta ccagccaaac cgctatctta cacattcgtc ctaataaacc
    10441 tcaagaaatt gcggctatcc agtggatggc ttatgggtca atgccattta ataccatggt
    10501 gccttttttc acacaggtga agactattcc agattatttt gccaacactt acgaaaatgt
    10561 gtctaccgac aatttctatt ggaccaaccg tctgattgcg gctttggcag atcctcacta
    10621 caatcatcat gaaactgatt tggacaatta cttggaagaa accatggcaa aaggtcatgc
    10681 catgttacat gctgtggaag cacagttatt ggctggtgaa acagtcgatt tggaagagga
    10741 aaatcaaaaa atgagtgact atgtgcaagg agaaacgcag acgctgttga acaagattct
    10801 ctttgacgct agcaatctaa tgaccaatcg tttcagtttg agcgattaaa agtgaagcta
    10861 aggctgagca actgcttagc cttttttacg ataacttttt aggaaaagta atcgcaaaaa
    10921 gacaaaaact tggaaaaaaa atcaaagtat gctatactta actggtcaat ttttaaccag
    10981 ttaataacaa aggaggaatc atgaaaaaga aaattctttt aatgatgagt ttaatcagtg
    11041 tcttttttgc ttggcaactt actcaggcaa aacaagtctt agcagagggt aaagtgaagg
    11101 tggtgacaac tttctatcct gtttatgaat ttacaaaagg ggttattggt aatgatggcg
    11161 atgtttccat gcttatgaaa gcaggaacgg aacctcatga ttttgagcct tctacaaaag
    11221 acattaaaaa aatccaagat gcagatgtat ttgtttatat ggatgacaat atggaaactt
    11281 gggtttctga tgtgaaaaaa tcattgacat ctaaaaaagt gaccatcgtc aagggaactg
    11341 gtaacatgct cttggtagca ggagttggac atgaccatca ccatgaggaa gctgacaaaa
    11401 agcatgagca taataaacat agcgaagaag gacacaacca tgcttttgac ccacacgtgt
    11461 ggttgtcacc ataccgtagc attacggtcg ttgaaaatat tcgcgacagt ctttcaaaag
    11521 cttacccaga aaaagcagag aacttcaaag ccaatgccgc tacttatatt gaaaaattaa
    11581 aagagcttga caaagactat acggcagcac tttcagatgc taagcaaaag agctttgtga
    11641 cacaacacgc agcttttggt tatatggcac ttgactatgg cttgaaccaa atttctatta
    11701 atggtgtcac accagatgca gaaccatcag caaaacgtat tgctactttg tcaaaatacg
    11761 ttaaaaaata tggtatcaaa tacatttatt ttgaggaaaa tgcgtcaagt aaagtcgcaa
    11821 aaaccctagc taaagaagca ggagttaaag cggctgtgct tagtccgctt gaaggtttga
    11881 ctgaaaaaga gatgaaagct ggccaagatt actttacggt catgcgtaaa aaccttgaaa
    11941 ccttacgctt aaccactgat gtggctggta aagaaattct tccagaaaaa gacacgacta
    12001 agacagttta caatggttat ttcaaagaca aagaagtcaa agatcgtcaa ttatctgact
    12061 ggtcaggtag ctggcaatct gtttacccct atctacaaga tggtacttta gaccaagttt
    12121 gggactataa ggctaaaaaa tctaaaggta aaatgacagc agccgagtac aaagattact
    12181 acactactgg ttataaaact gacgtggaac aaatcaaaat caatggtaag aaaaagacca
    12241 tgacctttgt tcgtaatggt gaaaagaaaa ccttcactta cacatacgcc ggcaaagaaa
    12301 tcttgaccta tccaaaagga aatcgcggag ttcgtttcat gtttgaagct aaagaaccaa
    12361 atgctggcga attcaaatac gttcaattca gtgaccatgc cattgctcct gaaaaagcag
    12421 agcatttcca cctgtactgg ggtggtgaca gccaagaaaa attacataaa gagttagaac
    12481 attggccaac ttactacggt tcagacttat ctggtcgtga aattgcccaa gaaatcaatg
    12541 ctcattaatc ttaactcact aagcaaatct tcttttaagg aggtttgctt tttctttgtg
    12601 gagtaaaaaa atttaataat tctcaacatt aatttgacgg acacttatga ttataatata
    12661 ctttaagtgg ttataacaag ttagaggtga ataagatgcc aaaagaacaa ccactctatc
    12721 tacagattgt tgatgacttg gaagtgaaaa tcaaaaagag catgacagaa aacgataagt
    12781 tgttgtcaga acgagaatta agtgaccttt acggtgttag tcgtatcacg attagactgg
    12841 ctcttaagga attggaatta cgagacttaa tttataaaaa gcagggcaag gggacttatg
    12901 tttcaggaat caaagaacct gtcactgatt tatcaagtac ttacagcttt acagagcaaa
    12961 tgaaaaaagt ggggaaaact cctaaaacag agattctttc ttttgaacag tatcaggtaa
    13021 caccttactt gtctggtctc ttagagtttg atctcgatac agaagttttt gaattagaac
    13081 gcctaaggat tgcagatgat atgcccttga tgtttgaacg atcctatatt cctgcgcagc
    13141 cttttcaggg gttaagcatt gcggacttga agcgtaaagc tttgtacgat atctttgcaa
    13201 aggactatca agagacaatc cgtcttgcag aagaagagtt ttatgccagc attgccttgg
    13261 actatgaagc agaccttttg ggaatcaaaa aaggagatcc tgtcttacat attattcgta
    13321 aaacttataa cgataaaaat attttgatcg aatttacttt cagtattgca agagcagacc
    13381 agtttaggta tagggtacaa catcacccca atggttaaag aaatgggagg ttaatacatg
    13441 tttacaaaaa cgcaggaagc tttggaggct cttggtgcag ctatcactac caaggaaatt
    13501 aaacaagaac cccgtttatg gcaagaaacc atgacgtttt ttgaagaaac aagagattct
    13561 ctggattctt ttttaaagag agtttgtaaa agcgctaatg gaaataacgt tcatgtcatc
    13621 tttacaggtg cgggtacatc tgagtacatc ggtaatacca tttgccctta cttgaagaag
    13681 gtggggaatc ggcagtgcta tctctttgag agtgttgctt caaccgattt ggttgcggcg
    13741 ccagattatt atcttgctga ggaagagacg gtactattag tctcctttgc taggagtggt
    13801 aatagtccag aaagtgtagc ggctgttaac ctggtcaatc aactagtgcc aaatagttat
    13861 catttaacca ttacgtgtgc caaagatgga gaactggcga aaaaagcaca gcaagatgag
    13921 cgttcttacc tttatttgat gcctgaagac gctaatgatg ctggttttgc catgactggt
    13981 agttttacct gtatgatgct agcggctctt ttgatttttg atgatgctaa cagtatagct
    14041 cagaaagaag cttatgtgac tgatatgatt cttgctgggc aagctgttat tgaccaagaa
    14101 agacggctac aagagctagc tgatttagga tttgaacgtt tagtttatct aggttctgct
    14161 ggtttagcca agttaaccca agaagctcaa ttaaaaatgt tagaattgac agctggacaa
    14221 gttgcgaccc tctacgagtc ctctatgggc tttaggcatg gtccgaaatc ttttattaat
    14281 gaccgtacct tagtcattgg atttgtcaat aatgatgctt acgtcagaca gtacgatttg
    14341 gacatgttag aggaaatcca agcagatggc attgccctta aaatcttggc tctattgcaa
    14401 ggaggggata tcaacttttc agaggaccaa tttcgactag acacccgtca cttattgcca
    14461 gaagcttact tagcttttcc catgatttta gtcgcgcaga ccttggccct cttaacggcc
    14521 gttacattag gaaattcccc tgacacccca tctgcgacag gaacggttaa tcgtgtggtc
    14581 aaaggagtaa cgattcaccc ttacccgact agtagtcgtc aatgcaacaa agcttaaagc
    14641 ttttgtgtag aagccgtaaa acgaaatgat tttgtgctag taggccaaaa gaaaaaccct
    14701 cctgatacag gaaggttttt tgtattgtct tgcttatttt gctgcgtttg cgtctttgag
    14761 accgtatttt ttgttgaagc ggtccacacg tccatctgct tgagtgaact tttgacgtcc
    14821 tgtgtagaat gggtgtgaat ctgatgaaat ttccacacgg ataagtgggt aagtttcacc
    14881 ttcaaactca acagtttctt tagaagcttt tgttgagccg ctaaggaact ggtaacctgt
    14941 agttgtatca aggaaaacaa ctggacgata atctggatga atgtcttttc tcatcttaaa
    15001 aaattccttt ctgccatgac acttgatgca tcatagttat taactattct agtgtaacaa
    15061 aacttgaatg acttgacaag gcttttcttt ccttattttt taaaaatcct aaatctcttg
    15121 gcaaacagcg atgagttctc ggaaaatagc ttgattttct tctaaattag ctgagttggc
    15181 tccgctagca aggggatgcc cccctccacc gtgacgctta gcaatgccat taataatctt
    15241 ttctttactg cgcatacgca cacggtagtt gccgtcagtt aactcaacaa aaatagccca
    15301 agcttgaacg ttatcaattt taccaggagc acagacaatg gcagagcttt ctgctagggt
    15361 cacgtcaaaa tgttttaagg tttcttggct gacaaggaca taagcagccc cactctcatc
    15421 aattgttaaa tgctcaaaga cgtagctttg cagctttgct attttcaaag gaaacgaatc
    15481 catttgcctt gaaatcgcag caaagtcgaa ctcgaaatgt ctgagttggc tagcaatgga
    15541 aagggtttta ctagtggttg aggcataaag aaatcgccct gtatcaccaa cgataccagt
    15601 gtataagagc ttagcagcct tgtcagagag agtaagattc tgactaaagg caaagtctgc
    15661 aatgatttca cttgcgctag aagcgcttgt gtccacataa tagaagtcac catatacatc
    15721 atcgttggga tggtgatcaa tcttaattaa gcactgcccc agtgtgtagc gctcatcatc
    15781 aatccttggt cgattcgctg tatctgtaat gatgaccaaa gcctctttat agtctttatc
    15841 agtcacctga tccatttggc taatccaagt taaactaggc tcatcaaaac cagtcatcaa
    15901 aaccttttta tctgggaaat tttgtgcaat aatttctttc aagccggcct gactaccaag
    15961 agcatcaggg tcaggatttt gatggcgatg gataataata gtttggtgag cttttatttt
    16021 atctaaaatt gtttcaaaag ttgttatcat gctttaatct ttctttttca aaaagaggaa
    16081 aataaccgtt taacataatc ttcaaaaaac ggtttgtgtc ataaggaggt gtgcgttatg
    16141 ttaagtggct aatgctagaa ttttttaaga ttgtcaaagc tcataaattg acattttatc
    16201 tgaaaatcat atcctatttt attatagcat aaaagtaagc tctagataat ggtaaattaa
    16261 tctaggaaga aaagaggtta gttatcagtt atgatactat caaagctagt tttgcaaatg
    16321 caagtgaaaa aatagttttt aagtgtctta agtaatcata tcaactttct ctttaaacaa
    16381 ttagtgcttt tcctaaaaaa gtgtgctaaa ataaacatag tttagtaatt ttgggaggaa
    16441 tttatggcat tagcaaaaat cgtttatgcc agcatgacgg gaaatactga agaaatcgct
    16501 gatatcgttg caaataaatt gcaagaattg ggccatgatg ttgacattga tgagtgcaca
    16561 acagttgacg cttcagagtt tgagaatgct gatatcgctg tcgttgcgac ttatacttat
    16621 ggtgacggtg acttaccaga tgaaatcgtt gatttttacg aagatttaca agatttagat
    16681 ttagaaggta aaatctatgg cgttgtcggc tctggtgaca ctttttatga ctatttttgc
    16741 aaatctgttg atgacttttc agatcaattt gcgcttactg gtgccgtcaa aggtgcagag
    16801 ccagtcaaag tagatttggc agcagaagat gaagacatcg atcgtttaga ggcctttgca
    16861 gagcaactct cacaggctgt taataatcta taaaactatt tagtaaaaat gtcagggact
    16921 tccttggcat ttttagattt ttaacctaaa ataaagggaa tgtcacttca gggtaggaat
    16981 tgaaaaaaca atcagcttca ccttgcttct gatcctctta ttaactataa taaaaagata
    17041 atctaataca tctagaaaag gaaagttatg cgtttagaag agatacgaca agaaatcaat
    17101 ggtattgatc accacttggt tgccttatta gaaaagcgga tggcacttgt agagcaagta
    17161 acagcttata aactagccaa tcacttgcct gtcttagatc aggcgaggga aaatcagatc
    17221 ctagataggg tttcttatct cgttaaagac caagcatttg agccagctat ccatgagact
    17281 tttaaaacca ttatgtctct ttcacggcag tatcaaacgc aacgcttaac aggtggtgac
    17341 attaatgatt aaatcaattg caacactgcc taaaccctcc ttaaaagaag cgttaggact
    17401 aataggcttg gcgcttccca taggggccac ggttggagtg gttgatgtga tttttggtaa
    17461 agggcttttg tttctttccg aatatcgcga tcaccacctg ttcttgcttc ttccttttct
    17521 tgccctagca ggtcttgtta tcgtttttct ttacgataag ttaggcaaag aggttcgtca
    17581 agggatggga ttggtttttc aggtaggaca tggtcaaaaa gatcagattc ctcctattct
    17641 cattcctctt attttgttct cgacttgggt aacccactta tttggagcga gtgcgggtcg
    17701 agaaggggta gccgttcaaa taggagctac catctctcat tattgtcaga gatttgtgac
    17761 tagtcaagaa gccgcacgac accttttgat tatggggatg gcagccggtt ttgcaggact
    17821 ttttcaaacg ccgattgctg ctgtcgtatt tgcccttgag gttttgttgg taggaacgct
    17881 tcgctactgc gctttattgc cttccttagt tgctgcttgc gtggcttctt ggacctctca
    17941 ttctttggga ttagagaaat ttacgattgt tcttgaagaa acattgacca taactccctt
    18001 aacattggtt aaattaattg ggttaggcct tatttttggt ctggtgggaa atagttttgc
    18061 ctatttactt ggctggttta aaccttacct cagtcaaaag ctaccaaatc cttatttccg
    18121 gattgctttt atcggtgctt tgttatctat ctgtctgatg attggtcacg tcggaagata
    18181 tagtggtcta ggaactcatt tgattgcggc agctttttct ggccaaacta tcctaactta
    18241 cgactggctt ttaaaaatga tcgtgacggt gattagctta tctgcaggct ttcaaggtgg
    18301 tgaagtgacg cctctatttg ctattggagc ttctttaggc atcgttttgg ccccttactt
    18361 aggtcttcct gtactgttgg ttgctgcact tggttacacc acggtatttg gaagtgcaac
    18421 aaataccttt tgggccccta tctttattgg gatagaggtc tttggtccag agaatgccct
    18481 agcatatttt gtcacctcgg ctgcagccta tatggttagt cacagacatt ccatttacag
    18541 ttatcaaaaa gtagtcatga tcaatttaaa agacatgacg gtaaatcaga tggatttaaa
    18601 aaaatagctt tacaataaag aagctttttg ttagaataaa tctcgtgtgt aatggacaca
    18661 cgaaaattac ggctaatccg ctaagacaag tacttaagat tagtaagata ggagaataaa
    18721 aatgaatcca ttaatccaaa gtttgacaga aggtcaactt cgctctgata tccctaactt
    18781 ccgtcctggt gacactgttc gcgttcacgc aaaagttgtc gaaggaactc gcgaacgtat
    18841 ccagatcttt gaaggtgttg ttatttcacg taaaggtcaa gggatctcag aaatgtacac
    18901 tgttcgtaaa atctcaggtg gtatcggggt agagcgtaca ttcccaatcc acacaccacg
    18961 tgttgacaaa atcgaagtta tccgccacgg taaagtccgt cgtgcgaaac tttactactt
    19021 gcgtgcatta caaggtaaag ctgctcgtat caaagaaatt cgtcgttagt ttgaaccgaa
    19081 acgtaacgta atagaataaa aagactagag tctctagtct ttttatgtcg tcgtgacaat
    19141 gtgacaattg gtgattaacg ctatgctcgt aggacacttt gaaagttcct tagcagttat
    19201 aattagtata ttgtaataac aaaatgaagc aaaataaact ttcagttatc tgatatttca
    19261 tcttgcatta ccttattttc taggttaaaa tagaagacaa ggtgaatata agataggccc
    19321 cttagttcaa tggatataac aactccctcc taaggagtag ttgctggttc gattccggca
    19381 ggggtcatta ttgtcactga aaaagccttt acatcaaagg ctttttgttt tattccggtt
    19441 ttaaaagggg cataaaaggg gcagtttgtt tatttataat ttctttcatg tttacagttg
    19501 tgtgactgta aatagataat gttgtttttg gatcgctgtg accaactcta tccattatcg
    19561 catttagcgg tatccctttt tctgctaaaa atgatatatg cgagtgtcta aataagtgcg
    19621 tgtgataatc tccgtaaatt ttcaatcgct tattgatgtg tgcatttaaa attggtaaac
    19681 cttttgaata tggaaagctg ttagcactct gcttgtgatt aatctatcgt atgttaaaag
    19741 tgacatttaa attaaatcat tttttataca tatatttcaa caggatttat catattggaa
    19801 aatagcggtg cttctattgg cattttacta ttgacaggaa tttccctgtc ttttgtctcc
    19861 taaagaagat cagctttgat ttctaacttg attagagtgg gcttggtact gtttatgaat
    19921 tatcagtata tactttctgg agatcaaaaa ggaaaggaac agctaaagag gtgtatgtgt
    19981 taccattaga ggaatagaac atcgcaagac tcgtttattc agtcattacg atacctgttt
    20041 tatttgggaa ataactataa tattaagtga atttgtgata taatgaaagg tatgaattat
    20101 caagattata tatgggattt gggaggtacc ttactcgata attacgagct ttcaacccaa
    20161 gcttttgtcc aaacgctagc ttttttcagc cttccaggag atcacgatgc tgtttaccaa
    20221 aaattaaaag aatcaaccgc tatcgctgtg gcaatgtttg ctcctaatga gccagaattt
    20281 ttgcatgtgt ataggctaag agaagcagac aaattggcac aacccatttg gtgtttggga
    20341 gctaaagaaa ttttggaaaa gatagcgact tcaggttcgc gaaatttttt aatttctcat
    20401 cgagattgtc aggtgaacca acttttagag caagctgggt tgctaatcta ttttacagaa
    20461 gtcgtgacag cttcaaatgg ctttgctcgg aaaccaaatc ctgaaagttt gttttattta
    20521 aaagaaaagt atgatattaa tagtggatta gtaattggag atcggctaat tgataaacaa
    20581 gcaggacaag cagcgggctt taataccttg cttgttgacg gtagaaaaaa cctattggag
    20641 atagtaacgt agatgattga agaaaataaa cattttgaaa aaaaaatgca agaatacgat
    20701 gccagtcaaa ttcaggttct agaagggctg gaggctgtcc gcatgcgtcc agggatgtat
    20761 attggctcga cagctaaaga gggtttgcat catttagtct gggaaattgt tgacaactca
    20821 attgacgaag cattagcagg ttttgcctct catattaaag tctttattga agcagataat
    20881 tccattacag tagtagatga tggccgtgga attccagttg acatccaagc caagacagga
    20941 cgtcccgccg ttgaaacagt ttttacagtc ttacacgcag gtggtaaatt tggcggaggc
    21001 ggctataagg tttctggagg attgcatggt gtagggtcat ctgttgttaa tgctttatca
    21061 acacaattag atgtgcgtgt ttataaaaac ggccaaattc attaccaaga atttaaacgc
    21121 ggggcagttg tagcagatct tgaggtcatt ggaaccactg atgtgactgg aacaacggta
    21181 cactttacac ccgatccaga aatttttacc gaaacgactc agtttgatta caatgtttta
    21241 gcaaaacgta ttcaagagtt agcctttttg aatcgtggtt taaaaatttc cattacagat
    21301 aagcgctcag gtatggaaca agaagaacat ttccattatg aaggtggaat tggttcttat
    21361 gttgaatttt taaatgataa aaaagatgtt atctttgaaa cgcccatcta tacagatggt
    21421 gaattagaag gtattgcagt tgaagtagcc atgcaataca cgactagcta tcaagaaaca
    21481 gtcatgagtt ttgctaataa tattcatact catgaaggtg gaacgcatga acaaggcttt
    21541 agagcggctc ttactcgggt catcaatgac tacgctaaga aaaataaaat tcttaaagaa
    21601 aatgaggaca atttgacagg agaagatgtt cgtgaaggtt tgacggcggt aatttctgtt
    21661 aagcatccaa atcctcaatt tgaaggtcaa accaaaacaa aattgggcaa ctcagaagtg
    21721 gttaagatca ctaatcgtct ctttagtgag gcctttcaac gttttctttt ggaaaaccca
    21781 caagttgctc gtaagattgt ggaaaaaggg attttggctt ctaaagctag aattgcagct
    21841 aagcgagccc gcgaagtcac ccgcaaaaaa tcaggcttag aaatttcaaa cttacctgga
    21901 aaattagcag actgttcgtc aaatgatgct aaccaaaacg aacttttcat cgtcgaagga
    21961 gattcagcgg gtgggtcggc caaatcaggt cgtaaccgag agtttcaagc tatcttgcct
    22021 attcgcggta aaattttgaa cgtggaaaaa gcaactatgg ataagattct tgccaacgaa
    22081 gaaattagaa gtctttttac cgctatgggt acaggttttg gtgcagattt tgacgtgtca
    22141 aaagctcgct accaaaagct ggttatcatg accgatgccg atgtggatgg cgctcatatt
    22201 agaaccttgc ttttaacctt gatttaccgc tttatgaggc ctgttctaga agctggctat
    22261 gtttatatcg cccagccacc tatttatggt gttaaggtcg gtagtgagat taaagagtac
    22321 attcagccag gtattgatca agaagaccaa ttaaaaacag ctcttgaaaa atatagtatt
    22381 ggtcgttcaa aaccaactgt tcaacgttat aaaggtcttg gggaaatgga tgaccatcaa
    22441 ctttgggaaa ctactatgga tcctgaaaat cgtttgatgg cgcgtgtgac agttgatgat
    22501 gccgcagaag cagataaagt atttgatatg ttaatgggag atcgtgttga accaagacgt
    22561 aatttcattg aggaaaatgc ggtttatagt acactggata tttagtcacc tttttagttt
    22621 cacaaggaat ctgctaccaa agaggacgaa ttgactaaaa atcatgatgc atgatgccaa
    22681 cagctttgtt agttagtacg tcttcatctt gagggacagc gttaatgcat aggtattgag
    22741 accgcccctt aatacctatg ttagtcctta ttaagaaagc cttgtgagtc gctggtacga
    22801 gattgttgaa ttagtcaaat atattttgaa atgcatttta aagtatgtta taatcgtaat
    22861 gttatagtgt attttaaata aaattttaat aaaaggcgat tgaggaattt cagtttttag
    22921 ttattactgg aacaatgttg ataaatcgtg tctaaataat aaggagattc agatgtcaag
    22981 cggaattatc ttgctgattg tggctatagt cctactagtg attatcgcct atctagtggg
    23041 tgtcatcata cgaaagcgta atgattccct aattaccagt ctagaagaac gaaaacaggc
    23101 cttgtttgct ttgcctgtta atgatgaaat tgaagaagta aagtcacttc atttgatagg
    23161 acagagccaa acctcttttc gggaatggaa tcagaaatgg gtagacttaa ctgtaaattc
    23221 gtttgcagat attgaaaatc atatttttga agctgaaaat ttgaatgata cgtttaattt
    23281 tattcgcgcc aagcatgaga ttaatagcgt tgaaagtcag cttaatcttg ttgaagaaga
    23341 tatcgcttct attcgtgagg cacttaatat cttaaaagag caagaagaaa aaaatagtgc
    23401 tcgtgtcacc catgctcttg atttatatga aaagctccaa gcttctattt ctgagaatga
    23461 agataatttt ggttcaacta tgcctgaaat tgacaaacag atgaaaaaca ttgaaactga
    23521 attttcacag tttgtcgcac tcaactcatc aggtgatcct gtagaagctt ctgaagttct
    23581 ggatagagca gaagagcata ctattgctct agggcagatt acagagcaga ttccagctat
    23641 tgtcgccaag ttggaagatg attttccaga tcagttagat gatttggaaa caggatatcg
    23701 ccgtttactt gaagaaaact atcattttcc agaaaaaaac attgaggcac gttttcagga
    23761 gattcgtgag tctattcgtg ccaattcatc agagttagtt acacttgact tggatcgtgc
    23821 tagagaagaa aacacgcata ttcaagaacg cattgattct ctttatgaag tctttgagcg
    23881 cgaaattgcc gcttataagg tagctgctaa aaatagtaag atgctacctc gttacctagc
    23941 acatgtgaaa cgtaataatg aacaactcaa agatgaaatt gcacgtttat cacgtaaata
    24001 tattctaagt gaaacggaaa gtctgactgt taaggcattt gagaaggata ttaaagagat
    24061 cgaagacagt acacttgctg ttgcagaaca atttggttta caagaaaaac cattttcaga
    24121 actgcaagtg acttttgaac gcagtataaa gacgcttact aatgtcgagt caggtcaaat
    24181 ggatgttttt gcagcagtta aagatattga aaaaattgaa tcacaggcac gccataacct
    24241 tgatgtttat gttactcagc ttcatatgat taaacgttat atggaaaaac gtcacttgcc
    24301 tggaattcca caagactttc ttagtgcttt ctttacaacg agttcacagt tggaagcttt
    24361 gatggatgag cttagtagag gacggatcaa tattgaggca gtgtcacgtt tgtcggaagt
    24421 tgcgacggtt gctattgcta atctagaaga tttaacctat caagtggttc aaaatgctac
    24481 cttgactgaa caattactac aatattctaa tcgctatcgt tcttttgaag caggagttca
    24541 aagtagtttt gaacatgctt tgagactttt cgaagtagaa aatgattatc aggcttcttt
    24601 tgatgaaatt tcttatgctc ttgaaacagt tgaacctggg gtaacagatc ggtttgttaa
    24661 ctcttatgaa aaaacacgag agcacattcg tttttgatac ttgaaagtcg tgatattctc
    24721 acgacttttt attagctttt tggaccaata cgtgtttttg actagaatct tctttttaat
    24781 gatcaaacga agaagtagta acagtgtttt ttggtattga gaggatagtt attttttcaa
    24841 aaatcttttc caaaaggatt tggttttttc aggaggttgt ttgctaagtt gatcattttt
    24901 tgtctgcttt aagatgactt ctaaacgcgt ttcctttaga tttattgcgt ggtcggtatg
    24961 aaggataaac gcaaaaggtg aagtcatatg cgtttctgta ataatagtaa caggtaaatg
    25021 gttttctttt gctaatttca tatagagtat ttgaagttga ggtgccagct tggaagagat
    25081 ttttaagtgt aaaggttgat agactaactg aaggctcggt aattctcttt tcaaatagtc
    25141 ttttactgtt ttagtttgag cttcgtctaa taaagcactt aaaacgactc gttcagcata
    25201 cgttcctaga tagtagcgtt gctgctcagg atttaatctt ctttctcccc aggctccttt
    25261 taatagctta tcttctaaat tagtcataga taccctcgct tctttataat agtttcttta
    25321 ttatatcgat ttctacaata aaataccaat ttgaaaattt tcggtaaatg tgttaaaaaa
    25381 caagaaagaa agtgattttt cttgcaaaaa acaaaggaag cttttcatga gattgctttt
    25441 taagttgatt aaaggcctag tttgtggtat tatgaacatg taaaatatta aaactcataa
    25501 ggagagtatt acaatgtcaa ttattactga tgtatacgct cgcgaagtcc ttgactcacg
    25561 cggtaaccca acacttgaag tagaagttta tacagaatca ggtgcattcg gacgtggtat
    25621 ggttccttca ggagcttcaa ctggtgaaca tgaagccgtt gaacttcgtg atggtgacaa
    25681 atctcgttac cttggtctag gtactcaaaa agcagttgat aacgttaata acattatcgc
    25741 tgaggcaatc atcggttacg atgttcgcga tcaacaagct atcgaccgtg caatgatcgc
    25801 tcttgacggt actcctaaca aaggtaaact tggtgctaat gctattcttg gtgtttctat
    25861 cgctgttgct cgtgcagctg ctgactatct tgaagtgcca ctttacactt accttggcgg
    25921 attcaacact aaagttcttc caactcctat gatgaacatc atcaacggtg gatcacactc
    25981 agatgcccca attgcattcc aagagttcat gatcatgcca gttggtgcac ctactttcaa
    26041 agaaggtctt cgttggggtg ctgaagtttt ccacgctctt aagaaaattc ttaaagaacg
    26101 tggacttgtt acagctgtag gtgacgaagg tggatttgct cctaaatttg aaggaactga
    26161 agacggtgta gaaactatcc ttaaagctat tgaagcagct ggttacgaag ctggtgaaaa
    26221 cggcattatg atcggttttg actgtgcatc atcagaattc tacgacaaag aacgtaaagt
    26281 ttacgactac actaaatttg aaggtgaagg cgctgctgtt cgtacatctg cagaacaagt
    26341 tgattacctt gaagagttgg ttaacaaata cccaattatt actatcgaag atggtatgga
    26401 tgaaaacgac tgggatggtt ggaaagttct tactgaacgc ctaggcaaac gtgttcaatt
    26461 ggttggtgac gacttcttcg ttacaaacac tgaatacctt gctcgtggta tcaaagaaaa
    26521 tgcagctaac tcaatcctta tcaaagttaa ccaaatcggt actttgactg aaacgtttga
    26581 agctatcgaa atggctaaag aagctggata tactgccgtt gtatcacacc gttcaggtga
    26641 aactgaagat tcaacaatcg ctgacatcgc agttgcaaca aacgctggcc aaatcaaaac
    26701 aggttcattg tcacgtacag accgtattgc taaatacaac caattacttc gtatcgaaga
    26761 tcaacttggt gaagttgctc aatacaaagg tatcaaatca ttctataact taaaaaaata
    26821 gacggtaaga cagaaatcgg tcattcgtag aatgcgattt cgtcgtcgca cccccgtcag
    26881 gctgactagg ttggtcacga caaaaggagt gaacatccag tcagctacga cgcaaaagtt
    26941 aaagattaaa gaatagaaag cttgggaaaa tcccaagctt tttgctttct aaatagtaaa
    27001 aatttactgt gggctaattt atcctgagct aatgtattag cgaggtaaag ttattgagga
    27061 aggcttggat ttgtactgcc tctaaaaagt tggacactat attttaaata caggactaag
    27121 tccttttata ccaattgtca agcacatcaa ggttttaact gaaaaaatgt tttagacaat
    27181 agcctaggtt tagctgttgt cttacttgtt gtgctctgat tagcataaaa aagtgtacaa
    27241 gaaaaaaacc ttgcctagta tttttaagat caaggtgtta caatgataga gcaacaacga
    27301 ctttaatgat tttgggtcga agcgtaattg taaagtttgt tatgcgttat gaggtaaagc
    27361 attg
//
LOCUS       DAUVUY010000012        26843 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.012, whole genome shotgun sequence.
ACCESSION   DAUVUY010000012 DAUVUY010000000
VERSION     DAUVUY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26843
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.012"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..425)
                     /locus_tag="VOX58_000511"
     CDS             complement(<1..425)
                     /locus_tag="VOX58_000511"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HES5877765.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATIT"
     gene            complement(490..747)
                     /locus_tag="VOX58_000512"
     CDS             complement(490..747)
                     /locus_tag="VOX58_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HES5877766.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            complement(1013..1429)
                     /locus_tag="VOX58_000513"
     CDS             complement(1013..1429)
                     /locus_tag="VOX58_000513"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HES5877767.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            complement(1442..2848)
                     /gene="atpD"
                     /locus_tag="VOX58_000514"
     CDS             complement(1442..2848)
                     /gene="atpD"
                     /locus_tag="VOX58_000514"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HES5877768.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDDLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            complement(3010..3885)
                     /locus_tag="VOX58_000515"
     CDS             complement(3010..3885)
                     /locus_tag="VOX58_000515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HES5877769.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            complement(3901..5409)
                     /locus_tag="VOX58_000516"
     CDS             complement(3901..5409)
                     /locus_tag="VOX58_000516"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HES5877770.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            complement(5425..5961)
                     /locus_tag="VOX58_000517"
     CDS             complement(5425..5961)
                     /locus_tag="VOX58_000517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HES5877771.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQTVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            complement(5961..6455)
                     /gene="atpF"
                     /locus_tag="VOX58_000518"
     CDS             complement(5961..6455)
                     /gene="atpF"
                     /locus_tag="VOX58_000518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HES5877772.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            complement(6473..7189)
                     /gene="atpB"
                     /locus_tag="VOX58_000519"
     CDS             complement(6473..7189)
                     /gene="atpB"
                     /locus_tag="VOX58_000519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HES5877773.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            complement(7224..7421)
                     /locus_tag="VOX58_000520"
     CDS             complement(7224..7421)
                     /locus_tag="VOX58_000520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HES5877774.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            complement(7813..8835)
                     /locus_tag="VOX58_000521"
     CDS             complement(7813..8835)
                     /locus_tag="VOX58_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HES5877775.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            complement(8849..10807)
                     /gene="ligA"
                     /locus_tag="VOX58_000522"
     CDS             complement(8849..10807)
                     /gene="ligA"
                     /locus_tag="VOX58_000522"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HES5877776.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWNFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKEDFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRQL"
     gene            complement(11001..11501)
                     /locus_tag="VOX58_000523"
     CDS             complement(11001..11501)
                     /locus_tag="VOX58_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HES5877777.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     regulatory      complement(11499..11614)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            complement(11779..14511)
                     /locus_tag="VOX58_000524"
     CDS             complement(11779..14511)
                     /locus_tag="VOX58_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HES5877778.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTSLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVIKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPCLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVNRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     gene            complement(15083..16201)
                     /locus_tag="VOX58_000525"
     CDS             complement(15083..16201)
                     /locus_tag="VOX58_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5877779.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSGQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGGTLSQAWPYLLALMGTALALISFSSLLLKPTKL"
     gene            complement(16198..17325)
                     /locus_tag="VOX58_000526"
     CDS             complement(16198..17325)
                     /locus_tag="VOX58_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5877780.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            complement(17334..18257)
                     /locus_tag="VOX58_000527"
     CDS             complement(17334..18257)
                     /locus_tag="VOX58_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5877781.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            complement(18280..18963)
                     /locus_tag="VOX58_000528"
     CDS             complement(18280..18963)
                     /locus_tag="VOX58_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HES5877782.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDV
                     LLSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIAILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            complement(18960..19631)
                     /locus_tag="VOX58_000529"
     CDS             complement(18960..19631)
                     /locus_tag="VOX58_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HES5877783.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELVFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKGVT"
     gene            complement(19606..20964)
                     /locus_tag="VOX58_000530"
     CDS             complement(19606..20964)
                     /locus_tag="VOX58_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HES5877784.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            complement(20984..22042)
                     /locus_tag="VOX58_000531"
     CDS             complement(20984..22042)
                     /locus_tag="VOX58_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HES5877785.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPEMFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNAPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQILPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            complement(22039..22989)
                     /gene="sagB"
                     /locus_tag="VOX58_000532"
     CDS             complement(22039..22989)
                     /gene="sagB"
                     /locus_tag="VOX58_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HES5877786.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            complement(23211..23372)
                     /gene="sagA"
                     /locus_tag="VOX58_000533"
     CDS             complement(23211..23372)
                     /gene="sagA"
                     /locus_tag="VOX58_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HES5877787.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            23935..24051
                     /locus_tag="VOX58_000534"
                     /pseudo
     CDS             23935..24051
                     /locus_tag="VOX58_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(24237..>26843)
                     /locus_tag="VOX58_000535"
     CDS             complement(24237..>26843)
                     /locus_tag="VOX58_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HES5877788.1"
                     /translation="KSGDPIEARRGLHNKSIDEVAQATKDAITADTTLTEAEKETQRG
                     NVDKEATKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGKGLDVRKDEHKKALEAVA
                     KRVTAEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKDADEADKAYGDGVTAIEN
                     AHVIGKGIEARKDLAKKDLAEAAAKTKALIIEDKTLTDDQRKEQLLGVDTEYAKGIEN
                     IDAAKDAAGVDKAYSDGVRDILAQYKEGQNLNDRRNAAKEFLLKEADKVTKLINDDPT
                     LTHDQKVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGIENINNQYQHGDGVDVRK
                     ATAKDDLEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKNTAIAAVDKATTADGVNQ
                     ELGKGITAINKAYRPGEAVKARKEAAKADLEKEAAKVKALIAKDPTLTQAAKDKQTAA
                     VDAAKNTAIVAVDKATTAEGINQELGKGITAINKAYRPGEGVKARKEAAKADLEKEAA
                     KVKALITNDPTLTKADKAKQTEAVAKALKAAIAAVDKATTAEGINQELGKGITAINKA
                     YRPGEGVKARKEAAKADLEREAAKVREAIANDPTLTKADKAKQTEAVAKALKAAIAAV
                     DKATTAEGINQELGKGITAINKAYRPGEGVEAHKEAAKANLEKVAKETKALISGDRYL
                     SETEKAVQKQAVEQALAKALGQVEAAKTVEAVKLAENLGTVAIRSAYVAGLAKDTDQA
                     TAALNEAKQAAIEALKQAAAETLAKITTDAKLTEAQKAEQSENVSLALKTAIATVRSA
                     QSIASVKEAIDKGITAIRAAYVPNKAVAKSSSANHLPKSGDANSIVLVGLGVMSLLLG
                     MVLYSKKKESKD"
BASE COUNT         8394 a         5480 c         4480 g         8489 t
ORIGIN      
        1 gtaatcgttg cccccatagc ctctaatcct tttaagtgca aattaatagg cctactgcca
       61 atcgtacagc ctccaggcat tgatactttt gcgtggccat tacgtgccaa aattggtcct
      121 aaaacaacaa tagaggcacg catttggctc acgtactcgt aaggggcttc atctaaaatg
      181 tgtcctgagg catcaactgt gatttcgtta gctgcttcat taaaatcaac acgaatatct
      241 aaaccacgta ccacgttatt catggtaaaa acatcagata gaataggaac atttctaaga
      301 attgtttttc cttttgaagg taaaatagat gctgccagta aaggaagaac tgcgttttta
      361 gcaccctcaa taacaacttc tccttctaag cgtgtttgac caccttcaat gattatttta
      421 tccacaattt tctcccttat ctatctctta gtcgtagtaa aattatacca tacttctttt
      481 attccagtat tagcggaact caaaaaagag cgtctggcta atttgaagca attccagcat
      541 acaatggctc accaaatacc ctagggcagt agctaataaa aagacaaaaa gtcgtagccg
      601 gccaagattt tctggatgat aatgactaaa ttttgtccag tcaatcacac taatcagtaa
      661 ttggtaacta aggccaataa aaaataaatg acacgaaaag gcaatgatct tcattaataa
      721 ttccatagga ttattataac ataccatctt gatccgtcca acttatcaat taagttgaca
      781 tcttctctga ctaagttgat agcctaaaaa tacattaagc gtcattactg agtttctact
      841 aacaacgtgg atacaaaaaa gagttccccc atataactcc tctcaacaat aataacgtgt
      901 ttagagaagg tagtatggtc aaactctcag aaactgatgt taaaaagact cagcaaacaa
      961 aataataggt taaaaacctt tcttacttat ccttgtcttt tatcaacacc acttactttt
     1021 tactaacgtt gatacgattc aaagcgcggc gcaaggcaac cttggcacga cgaacttcat
     1081 caatgttgtg agtcgtttca gcctgtgcaa tttcacgttc tgctcttagt tttgctcgct
     1141 ctgctcggct gacatcgata tcacggtcac gttctgcaga atcagcgaca acggtaacca
     1201 cattatcttt gatttcaata ataccaccat tgatagcaat ccagtccact ttttcatctt
     1261 caccacctcg gcgaattttc atttcatgga cttgcaaagg tgcaattaaa ttgatgtgat
     1321 taggtaaaat ccccatttcc ccatctggtg ttgttaccga aatacattta gcatggtgat
     1381 catatttgat tccatctggc gttaccactt gaacagtcat ttgtgtcatg gcatcacctc
     1441 actaaaagcc cattttttcg gcttttttaa tcacatcctc gattggacca actgaacgaa
     1501 aggcgtcttc tggaagatca tcgtattttc cttcaagaat ttctttaaag ccgcgaactg
     1561 tctcagcaac tggcacgtaa gaaccaggaa gaccggtaaa ttgttcagca acgttaaaat
     1621 tttgtgagag gaagaactgg atacgacgcg cacgtccaac caatgttttt tcttcatctg
     1681 ataattcatc catccccaaa atagcgatga tgtcttgtaa ttcacggtag cgttgaagca
     1741 cacgttgaac ttctgttgcc acagcatagt gctcttcacc aacaatctca ggtgataaag
     1801 cccgtgaact tgaagccaat ggatcaacgg ctggataaat ccccatttgt gtcaatttac
     1861 gttcaaggtt agttgttgaa tccaggtgag caaatgctgt tgctggcgca gggtcagtat
     1921 aatcatctgc tggtacataa atcgcttgga tagatgtaac agaacctttt tgagttgatg
     1981 taatccgttc ttgcaattgc cccatttcag tagcaagtgt tggttggtaa ccaacagcag
     2041 aaggcatacg gcctaagagg gctgatactt ctgaacctgc ctgagtgaaa cggaagatat
     2101 tatcaataaa gagcaaaaca tcttggcctt ctacatcacg gaaatactca gcaatggtta
     2161 aaccagtaag ggctacacgc atacgtgccc caggcggctc attcatctga ccaaaaacca
     2221 tggcggtttt ttcaataacg cctgattctt tcatttccca gtaaaggtca tttccttcac
     2281 gtgttcgctc accaacaccg gtaaatactg aaatacctcc gtgttcttgg gcgatattgt
     2341 ggattaattc ttggataagg acagtcttac caactccggc accaccgaaa agtccaactt
     2401 taccaccttt aaggtaaggg gcaagaaggt caattacctt gataccagtt tcaagaattt
     2461 ctgatgatgt tgataattca tcaaacgatg gtgctttttt atggatcggc tgacggtcaa
     2521 catcttctgc aaatggttct tccaaatcaa tggtttctcc aagtacatta aagacgcgtc
     2581 ccaaagtttc tttacctact ggtacgctaa tcgcacgacc agtgtcaaga acttctaacc
     2641 cacgtgtaag cccatcagtt gattccatag cgattgttcg caccataccg tcaccaagtt
     2701 ccagagcaac ttcaaggacg attttttgct ttttatcact atctttataa acaatcaatg
     2761 cattattaat ctctggaagt ttgtccccac tcgcaaacat aacgtctact actggaccaa
     2821 caacctgagc aattttgcct gagctcattc atttctcctt atattcgata gtcagggctt
     2881 agttagtctt gctaacttct tttatactga aaagagcagt ctttcaatag aaaagtccca
     2941 taagtcattt catctcgatt gataaaattg aatgctgaca ataacctaat cgctagtcag
     3001 cgttattttt tattctagtg catttgctcc tgcaacaatt tcagtaattt cttgtgtaat
     3061 tgctgcttga cgagctcggt tatattggat ggtcaaatcg ttaatgacat tcttagcgtt
     3121 atctgtcgct gtctgcatgg cagtcatacc tgctgcatgt tcagccgttt tagcatcaat
     3181 gatagcccca taaattaaac tttctgtgaa ttgaggtaac aattgatcta aaatatcatg
     3241 acgattaggt tccaattcaa acccagtaac accttcttct gctgcttcat cagcaaccaa
     3301 gtcagaaatt ggcaacatct gttgaacacg gacttggctg gttaagctat tcacatggtg
     3361 attgtaacag acatataact catcaaaaat ctcattgaca aacatgtcaa cggattgtga
     3421 aatgatttgt ctcacttgtt caaaacttgg ttgatccgca agtccacgaa gttcaaaagc
     3481 cacattcatc cctcttgctt tgaagaagtc tgaaccaaca ctaccaatag aaatgatttc
     3541 atagtcacca tcagcatggt actctgtaat catatccata acagatttca agatttttga
     3601 attgtaacct ccaacaagtc ctttatcaga cgtgatgaca atatagcctg tttttttgac
     3661 aggacgagac accaacatcg gattatcaga tcctatggtt aattctgatt tcaataaatc
     3721 tgtagtaatc tggcgaattt ttgaggcgta aatttgaaaa tcacgagctg cttgctcaga
     3781 tttaacaagt tttgcagaag aaaccatacg catggcacta gtgattttac ttgttttctc
     3841 agttgaaata attttagctt taatttcact tagagagcct gccatactag tatcctccct
     3901 ctatgactta aagtttgagt gttctttaaa cgctttaata gcggcatcaa gggcagcttc
     3961 ttcaggaagg tcttttgtgg tacggatagt ttcaaataaa tcattgtaat gaacgtcaaa
     4021 gtagtcgtaa agagcttctt caaaagccaa aatatcatct acaggaacat catctaagaa
     4081 accatgagta agggcataaa gaataacgac ttgcttttca acaggtaacg gtttatgcaa
     4141 tggttgcttc aagatttcaa ccgtacggcg accacgatta agttttgctt gtgttgcagc
     4201 atcaaggtcc gaaccaaatt gggtaaaggc ttctaattca cgataggaag ccaaatccaa
     4261 acgaagagta ccagcaacct ttttcatcgc tttaatttga gctgaaccac ctacacgtga
     4321 gacagatgat cctgcatcaa tcgctggacg aattcctgaa ttaaagaggt tctcttgcaa
     4381 gaagatttgt ccgtctgtaa tcgaaataac gttagtcgca atatatgcag agatatctcc
     4441 ggcctgtgtt tcgataaatg gtagagctgt gatagaacct ccacctaagt catcagaaac
     4501 ttttgcagag cgttccaaaa gacggctatg caagtagaaa acatcccctg ggtaagcttc
     4561 acgacctggt ggacgacgaa gcaagagaga caattcacga taagccaccg cttgttttga
     4621 taaatcatca tagacaatca agacatgctt gccttggtac ataaattctt cagccatggc
     4681 aacacccgca taaggagcga tgaagagcaa tggtgatggt tgtgaggctg acgctgtcac
     4741 aacaatagta taatccaagg caccgtaacg gcgaagggtt tcaacttgtg tacgaacggt
     4801 tgattctttt tgaccaatag ccacatagat acaaatcatg tcttgacctt tttggttcaa
     4861 aatcgcatca atagctacgg atgtttttcc tgtttgacgg tctccgataa tcaactcacg
     4921 ttgcccacga ccaattggaa ccaaagcatc aattgctttt aatccagttt gtaagggttc
     4981 agaaacggat ttacgctgca taacaccagg agctggcgtt tcgactggtc taaagccagt
     5041 tgtttcaata tctcccaagc catcaactgg ttgtccaagt gggtttacca cacgtccaat
     5101 gagagcttca ccaactggta cttccatgat tttaccggtg cgcttaacca catctccttc
     5161 acggattgct gaaaaatcac caagaataat aatacctaca tcatttgact caaggttttg
     5221 agccatccca taggcaccat tttcaaattc aagaagttca ccactcatgg cattatctaa
     5281 tccacgggca cgtgcaatac cgtcaccaat ataggtaacg atcccagttt ctgtgacatc
     5341 gaaatttggc tggaagttct caatttgctt tttaattaaa gcgctaattt cttgtgcatt
     5401 aattgccaaa agtcacacca ctttctattt taaattcatt ttaaatgctt gcaattgacg
     5461 acgaatactg gtatcaatta ctttattatt gacgctgata ataaatcctc caataagcga
     5521 aggatctact ttttcaatta aacgacccgc tgttacagca aatttctttg caacaactgc
     5581 tcgaacacgg cttttttgct cctcagtcaa aggtagtgat gaggtaacag tcacatcata
     5641 ctgatttgaa actattgcaa tttcatttaa aacagtctga agcatttgat acaaataagc
     5701 ttcacgttcg ttttgtaaaa tgagattcaa gaaattattc atataaacag aattatttcc
     5761 ctttaataag gttagcaatt ttattttttc agcatgcgga acagctagac ttgatagact
     5821 ttgatcaaga ttagttgtta caaacgtctc taaaatcgca agcacatcag cttgtaaggc
     5881 atcaagagaa tgatgttcac ttgctacctc aacaaggctt tttgcatatt gctcgataag
     5941 agcctgttct tttttggtca ttaagcttct cctaagtcat caagataact gtcaataagc
     6001 tggctttgcg ccgtcttatc aagattggct cccataattt tttccgctaa aagaaccgtt
     6061 aaatcagaca tttctgtttt gactgctgaa atagcgtctg atttgctttg ttcaatatct
     6121 gtcaacgctt tttctttcaa gcgttttgct tcatcagtag cttctgccac caatttatca
     6181 ccttgtaatt gaccaatttc tttggcatca ctaatgattt tacttgcttg tgaacgacta
     6241 gcatctaggt tagcttgact ttttgcctct aactgttgag cacttaaacg tgattgctca
     6301 gcctgatcaa tatctcgaga gatttgttgt gagcgtgtct gtaaaatcga ttcaattgca
     6361 ccccaagcga attttttgat caagagtaac aagacgatta cagaacctgt tactagaata
     6421 aaattcccaa cgagttcacc aaatgtgatt gacattcgtt tgttctcctt ccttattctt
     6481 ctgattcatt aactttcttt cctagatagg tcgctgtcag ttttgtgaaa acaaaagcct
     6541 gtatgcagga aataaagact gaaaaggctg tccacgccat attgactagg aaagcgatcg
     6601 gccaccaata aactcgataa ttggccagtt gaacaatcaa acctgtaaca acttccccgg
     6661 caaagatatt accgaataac ctaatggcaa gtgataagaa atttgtgaac tcttctaata
     6721 aattcatcgg agtcattggc cacggtgtag ccaaacgttt caaatgggca accaagccac
     6781 gacgtctaac cccttcaatg tgtaccatta aggtaataaa aagagataga gcaaggtcaa
     6841 aagccagatt ggctgttggc gaggtccata ggttatagcc attaactgtc tctaacttag
     6901 taaataaccc taaattatta gcgacagcca caaagagaaa aatggtaaag agtaacagcg
     6961 aataagattt ttgtagatta tgatctaagt gctcttctcc aataccatcc acaaaactga
     7021 ttaaatattc taaagcagtt tgcttccctt ctggtttcaa tttcatttgg cgacttgccc
     7081 aaaaaacaaa ggcaaagata acagcaattg tgacaataca aacagcaagt agggtcaaat
     7141 taaaggttat tggaccaagc tttagcatgg gtattttagc ttcttccatc taacaccctc
     7201 cttttctatt atcaaatagg ctgttattta agaatgaagg ccataacaag ggtaacaaag
     7261 aaagtacctt cgataaaggc aacacccaag atcattaatg aacgtaattg accaatgatt
     7321 tctggttggc gagatgctgc tttaaaaaga tttgccatca agaaaccttc ggcaagagaa
     7381 acaccaaaac aggccaaagc caatgcgaaa ataggattca tagtagaata ctcctttaaa
     7441 ttttttttac tttgctattc tattccttaa aattaagaat gtcaacagat ttctttagac
     7501 tgagtacgct ttcttagact aatactaacc tttttgataa acgagtctgt ctttgttaag
     7561 atctttttaa aaatagaatt acactcttat tataacaaaa aggcaacaag attccaaaaa
     7621 gggtataata acatccctaa aacatggcat tttcttgcat atgatcaaaa aagagagacc
     7681 tcaaatgggc ccctcatatg aataaattga ctctttgagc acatcaaaaa acactacctc
     7741 aattaattta aaaatcttag attctaatag tagtgcattt gactaaactg tcagtagcta
     7801 ataactattg tattattctt ctaattcttg ttctaagtct tctacttcat gagcaaattt
     7861 ctttacgatt tcctcaattt ccaattcgtc ctgatctaag actaaagcgt catcagaaat
     7921 caaatcagta tctgcaaaaa aagtgatatg attttttagg ttctctaatg taattggagc
     7981 atctcctccg tactcgccat ctaaattaat catcattcgc ttaccacatt ggggttcaat
     8041 gactatttta ctggtcttaa tgtattcgac acgacgatct gtaatatgct taccaccatc
     8101 caaaatcaaa cgcaaaagat gtacaatttc aaaaagattt gctgtcttta ttaaaatgag
     8161 cgtaaacata ccgtcatcta gttttgcatc aggagcaatc atttcaaatc ccccaacaga
     8221 attggtaata gctgcaaaaa tcatagaaac ttgaccttca aagacgcctt tgtcatgcgt
     8281 aattttaaca ggcacattgc taactcttgg gagcagttct actcctttag caagataagc
     8341 caagtatcca aacatcgttt ttaactgact tggtacactg taagttaatt ccgtcaaact
     8401 tcctgctgca gcaatattga tgaaataagt atctttctta gctctaccaa tatccatctg
     8461 gatagtctga ttttttccga taagttttgc tgcttggctc ggatttcctc ttggtacttt
     8521 taacgctcta gcaaaatcat tagtggtacc tgttggaata atggccattt tagggcgctt
     8581 tttcaaggga gcaattccat tgaccacttc gttaatagtg ccatcacctc cagcagcaat
     8641 caataagtca aaacctgcct ttgccgctct tcttgcttca tttagagcag agttcttttg
     8701 ggctgttgtt tgaaaggcag atgtttcata cccgaaccct tctaatatgt ctaaaacttc
     8761 aggaacactt tttctcataa gttcttgtcc tgacgttgga ttataaatga gacgtgctct
     8821 caattgtttt ttcataacat tacctcactt aaagctggcg tagccaatct tcatcttcaa
     8881 tccgaattcc taagctcttt gctttttcta gcttagaacc agcgtcacta cctgcaataa
     8941 ctaaatctgt tttcttagat acacttccag taactttggc acctaatgcc tctaatttat
     9001 ctttggcctc atttcggttc agctgattta atttgccagt taaaacaaca gttagaccaa
     9061 aaagcgctgc gtcactatta actttttgac ctgagtaatg catgttgaca cctgctgtct
     9121 ttaactcgtc tactaaaatc gcagccgttt tttgttcaaa gtattgagta agtgattgag
     9181 caatcgttga gccaagacca tctatccttg cgatttcctc ttctttagcg gttaaaagag
     9241 cagaaatatc tccgtatacc tctaaaatta agcgactaac cttagagcca atatgacgaa
     9301 ttcctagacc aaataacagt ttttcagctg aatttgattt agaagcctca atcgcagcga
     9361 gcaatttatc agctgacttt tctttgatgc catctaattg catgaaatct tctttggtca
     9421 actggtaaat atcagcaaca tcatgaacaa accccgctaa aaatagtttt tccacaatag
     9481 ctggtcccag cccagtaatg ttcatggcat cacgacttgc aaaatgctcc aaacttcgct
     9541 gaatcaaact aggacacaac ggattaatac aacgcaaagc aacctcatct tcaaaatgaa
     9601 ctaattcact accacaagat gggcatagtt taggaataag catgacttct tgctggttcc
     9661 gcttactcat aacgacattt aagacagcag gaataatgtc ccctgcctta tatacaatca
     9721 ctgtatcgcc aatacggata tctttttcgg caatataatc cacattatgt agggttgcac
     9781 gactcacggt tgtccccgct agttgcacag gagttagatt cgctgtaggt gtgaccacgc
     9841 ctgttcgtcc cactgtccag tcaaccgaaa ggatttctgc ctctttttct tctgctggaa
     9901 atttgtaagc aatagcccag cgtggagctt taaccgtaaa ccctagctct tcttgcatgg
     9961 ctaggctatt aactttaata acaacgccat ctatgtcata agctaattga tctctttttg
    10021 cctcaatagt tttgataaaa ttccaaatct catccataga cgatgtgagt tgataatacg
    10081 gatttactga aaaacctaga tctgctaatt ctgctaagac ctcattttgt tgatttctag
    10141 ctgtcggact agcttcttga tacaaaaaag tggctagttg acgcttagca accactgccg
    10201 tatctagctg ccttagtgtc ccagcggcag catttctagg attagcaaat tcagtctccc
    10261 cattttcttg gcgtgcttca ttgatcgctt taaaggactg acgagacatg taagcctctc
    10321 ctcgaacggt gatggttaaa ggctcactca attggtgagg aatatccttt atttttttaa
    10381 tattttctgt gatattttca ccgatattgc catcaccccg tgttgctcca acctgtaaga
    10441 agccattctc ataactcaag gaaatagata aaccatcaat ttttaactct gctagatagg
    10501 tggcattcgg aaattcggcc ttgacgcgct tatcaaaagc atccagctcc tcacgtgaaa
    10561 aagcatcctg caaactaaaa agcggatact gatgtcgata tttttcaaac ccctttaaaa
    10621 tagtccctcc aacctgctgc gttgggctat cttgcaagac atactcggga tatgactgct
    10681 ctaaagtcac cagttcacga taaagcttat catagtcact gtcagaaact gatggggcat
    10741 ctttagtata atagtcataa cggtaacggt taagaagatc tgttaattct tttatgcgtt
    10801 ttttcatact tttattttat cataaaagat aacaaatgag ctatttctat atcataacag
    10861 catcaaaagt aatcgttggc cactccatat ggtactatta gctaaagtgt taaatatttc
    10921 tatctagaac gattttaaca ttacaaacaa cataaaaaag agaagggtat tgacccaact
    10981 cttttgagta tgatgcatta ctaaaaggaa attctttgag acaacttatc aataataagt
    11041 gatcctatga gcagagatac taattctccc aacgcagttg taaaccatgt taataaaaat
    11101 ggagctccaa agaagtaaag ttcgattgct acagtaaaca tggaagttgc aaaaaagaaa
    11161 gaaaagtaaa caaaagcttt attaaaaatg ccattaaaaa gataatcttt ttgatactta
    11221 ctaaacaaaa taacacctaa ggtcacaaaa ataagagttg aaccgccgcc tacaaagaca
    11281 tcaatcagtc cgaaactata aaagttagca atcatacatc caagagtcac cgcaataata
    11341 tacttgcgat gataaaaagc taagaaattc atcatttcag atattctaaa ttgatacatc
    11401 ccataagaaa tcgcatttaa aggcggggta atagttaaga caacatacaa ggcagctact
    11461 agcccaatgt gaacatagtc gtgtacagtc aattttgtca ttttttctcc tttaacgtct
    11521 ggataactct caaaagctat ctacgtttct aatatgcttg gtgaaaggga actaagcacc
    11581 aagggttgca aagcaacggt tacattgtac cataaaacaa ttagcttgac aaaagtcttt
    11641 agcaagtaaa gctgtcttat tgttatcttc ttttgttatc aatattagtt tttatatagt
    11701 catccatttt agcttaaggt ctaacgtaag tatatcaagc ctttaacgac tctttatgaa
    11761 ctccaattct tcaaatcgct atgattcctt tttgccttta gtcaataata aaataggcac
    11821 taataaacta gccgttccta gtagcgttat cacataaaca agactatctc ctgtttttgg
    11881 caacagtttt ccttttgaag tttttttagc ttttacactt tgtttagaag actctgcctt
    11941 atcattttct ttagaaaatg ataattgaag taacaatgga tcatgatctg atgcgcgtcc
    12001 gtgagcttcc ataaatgggg aattcacatg aaccatgtca aattcgtagt gatcaagtaa
    12061 atggcgtgaa actaatatat tatcaagggt ctgattattg ccttggtgaa aataagaata
    12121 ccgatctgaa atatcatgtc ggttcaccaa gttaaccata tcaccttctt cgattaattg
    12181 aatcgtcttt gtgaattcaa aatcattaaa gtcacctagc atcacaatat tagcttggtg
    12241 ttttgcccct tctttcgcaa attgtgctag catattagcc aagacgtgac gtctttgctc
    12301 agatttaaaa gtgactggtt gcacacgacc ataaagagca ttatccccac gcttagagtt
    12361 caaatgattt gcaacaacaa cgactttacg accttggaag ataaattctg ctgctagcga
    12421 tttacgaaca tctttccaag cggcgttagt tggatcaatt cgaccaacac taaggtttaa
    12481 ttctccatta acccaagtta gagcatcacg agcaccgcct tttggcttat cagaaaggct
    12541 gacgcgctct ggttgataaa ggaatcctgt tcgaatatta ccacctggtt gacctccgtc
    12601 aacattattt tctggagcaa tatcaacata acgataagtt gggccaccta gttttttaat
    12661 agcatcaatg aggcgttgcg cgctttgtgt cgcatccgtt gtcccatcat cagtcggccc
    12721 attattatct tggacttcaa ttaatccaat aatgtctgga gcattcagat catgaataaa
    12781 ggattcggca atccgtttga ccttctcatc tttagttgaa gaaggattgg ctgagaagtt
    12841 ttcaatattg taagaagcaa tgcttaactt gctaaggtct ttttgcaagt ttgttttttc
    12901 aggttttaga tgaccatcca ttaaacatgg catgtttttg ctgtcatcaa caaagacctt
    12961 gtaattacca tagctataag atactggccc agctaatctt cctttgtaag agtctcctgc
    13021 tttaataatt tgtttgcctt ttttgaaaag aacaggaatc acatctgtgt tataagaatt
    13081 agctggaagt aatactccac ctgaattatt taacggtctt gtactagagc caggtaagac
    13141 ataaatttct ttatttttca ttggaccaag gattttagca tcatcaaccg ccactaacat
    13201 gccttccatt gattcccaat aatcaatagc gtcttcttct ggatcaaaca cacgcaagcc
    13261 atcattatca ataatattgg ctggcgcgat acgatctttc cctaaaacaa gcggtgatgg
    13321 aacttgagct gtccctgttt tgatcactgc tttagccaca atttgggtga tggtaaggtc
    13381 agtctgctta cgttcctcat aaccacgacc aaagaattct tccacttcac ctgaaatggt
    13441 caaaacatcg ccgacttgaa ccttagcgtt tttagcaaaa acacgaatac catctgaagt
    13501 cgctaaatca ccatcaccat taagatcttg aacataaaaa tgagtggaat cgtctaaata
    13561 agtgacaaca acctgttcta cggtgaccgc ttttttgaga agtggcgacg tatgactagc
    13621 tccttgaatc tcgcctattt tcacaatatt acgagatgaa gcatcactat ttgagcttgt
    13681 gaccttttta accacttcca attgttcaag agaaaacggt cttaattgta actgaccatc
    13741 aacaatagac aatatggctg tgaggttaat caaatcaccc tgacttattt tagtaacaac
    13801 atcagcccct ttaacccctg tacgatgatc tatatgaaca actatacttt taccactatc
    13861 atcccttaca aggaaagatg tgttgttata ttgatcagtt gataattctc caactgtcac
    13921 gtgagataac ttaactaatg ttgagggtgt tgttgtttct aattctgata ttactgcttc
    13981 tttaacagaa gtcggaaaag ataactctaa ctttttgtgt gcagtcacct gttggagttg
    14041 taaatcacct ttaaagcgag taagtgtacc tgttaattga acaatatctc ccttactgta
    14101 tcctaaatct ttttgaggat aaacataaag tccaatacct tcactatctt gaatataaaa
    14161 gccatagcct ccccagccat tgatcacact cgtaatcttg ccagtaacag tataggttct
    14221 ttcttcaggc ccctttctta cttgagcaat agactgttca gtagttactt ttactccttt
    14281 atcaggtgtt ggcaaagatg tttttctctc accaaaatcc acacttgcta ggtaaggtga
    14341 tatgacggga aaattcgatt tatcactaat aacagattct ctctttggcc tttgtgttac
    14401 gatttcattg gcataagtca aattttggcg tgaagtaact tcttcaacac tagtaagcgt
    14461 aatagctagt gtcaacaatg aaacaggtat tatatatttc ttgttaatca tgatatctcc
    14521 ttttaatgtg attagtttaa gacaacaaca agattttatt tttgaatatt aacctccacc
    14581 tgagactatg aattgggata cactttctgt ttgcattaat tatactatca agtaacacga
    14641 ttagatcttt tcttttaatc tgttaagtca cccaattaaa gatttgtttg atctgacata
    14701 acatcacaag catccctgat agcgaacaag acagctttat agcattcgat acatgcttat
    14761 tgtaacaaaa acattccata aaataaatac gttttcacaa ctttttttaa taacattagt
    14821 ttttatgttt attgaaaacg tttttttaaa atactttgac aactaatctg tcaacaaaaa
    14881 cgaactttct agagaggtcg cttttgataa tatgctctct aaaaaacaca gtaacaacag
    14941 taacatcgca atcgctttta acaaaagata agtgcactag agaaaatata agcaaaaagc
    15001 aaccctttta ttgcatataa gccaataaaa gggctacctg gtagttagct aattaggaaa
    15061 gtatttttta acaaaagact aattatagtt tggtaggttt taataataaa cttgaaaaac
    15121 taatcaaagc tagtgctgtt cccattagag ctaacaagta tggccaagcc tgagataagg
    15181 tccctccact ttgaaaaata gctatggctg ataaaaccca attttgtggt aataacttac
    15241 caattgcttg catataagaa ggcgtaatag acaatggcca taaacagcca gccaacattg
    15301 aagtcggcat tacaataaga ttagctaact ggctactttg ttgggaattt tgtgtgatag
    15361 ctccaatcag caggccaaag cctatcgcta ataaactaaa aaggaaaaaa ataatcaaaa
    15421 gaagtgatgt cggaacaaaa aaactaatat taaatacttt taacaaactc agcataatga
    15481 caatttgaat cgtaaaagca acaaatccta cacagacgta actcaacata tagcgaaaac
    15541 gagataagtt cgataacatt aatcgatggt ataactgacc agatttatct gccaaaatac
    15601 cactgtaaat cacgctcgta ctacctaaca ttagaataag caaaaatcct gtcgttgtgg
    15661 aagatactgc tttgctacgt gatcggtctg tcaaagttac ttgcttaaca tcataattta
    15721 actgttggtt tttttggaga attctattaa aagtatcttc gttacctaga gccacatccc
    15781 ctataatatt atagttttcc aatagataat tggtctgtgc ctttacccac tctgtaattt
    15841 cgctattagc aatcgaacgt atattgagtt tttgagattt tccttgcaac actttatctg
    15901 agaatccttt gtcaatagtc aataccgctt ctagagattt atcttgaagg tagtgatcaa
    15961 tatgctcttt tttgattttg gtaaaaatgt cgtacttttt gttttgcttt agttgagcaa
    16021 taaattgttt agatatctga ctattatctt tatctaatat cccaatttta gcctgattat
    16081 tatttgaaaa actcaaagat aaagccaaaa gtgtcgttaa aataggcatt aaaaagaatg
    16141 tcacataact tgtttttttg cgctttatct taacaaatag agtttttata aaacaaatca
    16201 tgtcgtcacc tgctttctca tacctatcaa tgccaatatt ataaaaattg ttccgatact
    16261 aatattaacg atcaaagtta caggaatcgg attatattga ccaccaaaaa taagatagaa
    16321 caaactatca ttaacccatt taataggaga tatattcgac aattgattaa taatagagct
    16381 gtgtaatgtt gtcaatggaa cgtaactgcc tcctaaaaag gcgaatatag gaataatagt
    16441 attggatgca accgttaaaa aagcctcatt tttaatgctt atgcctagcc cgatacctat
    16501 agctacagag agatagacta aggaggctgt tataccaata agcatccact cattagtgcc
    16561 ccaattaact cgtaaaacaa accttgtaaa aatgtataaa agactcaatt gaacagttgt
    16621 tgctaacata gcacctatta attttccaaa gaccaaaaga cttggagaaa ctcctgttaa
    16681 gtgaatacga tttgtaatcc gttgttgacg atctgacaaa acaaggttca tcgcagacat
    16741 catactataa aaagtaatca tggtaaacat agaaatagca taataatcag ctgatgtcat
    16801 tcctttactt gtctgtattg aactgacctt aacatagtcc acacttcgct ttctgatatg
    16861 atttaattgt tgaggataaa gagctgctat cgagtcataa gttttagcat tatctacata
    16921 attttttact agcatattga tcaaatctgc attttgctga ttaatattat tagtaataac
    16981 cttaatggtc tgattcttct tcacttctaa aacagccgtt aaggctcctt cactgaccct
    17041 ttgtcgatct ttttcaagag aattactagg tttagcctct aaatgaagtt tttggtttaa
    17101 aacttttaaa aaagcaagaa aatttttctg gtaatccgtt ttttcaccct ctaattggta
    17161 acgaatggtc aatttaggga ctgttgtcgc agtattaaaa cttgatttaa aggcaaaact
    17221 taaaataacg atcatcaaaa ttggaaaaat caccatcatc aataaggctg ttcggtttct
    17281 aaaaatctgt aaactttctt ttttgattaa atgaaataaa accatagtaa cctctaatct
    17341 cgtaacttct tacctgttaa gtgtaagaaa atttcttcta aattcaaatg gttatgtcta
    17401 atctcgctaa aagtaatatt ggcctgtgtg agctgatgaa caactgatgt catatcacta
    17461 ttatcaatct tcaacatgag tttttctcca tcttctatca aagaccaatc aggcttatct
    17521 gccagttcca aatgacgtga atctgttaga gttacaataa tctggtttgc tagatttgca
    17581 acgtaacgtt tttccagttc aaatttaggt ccttcttcaa taacttgacc atgatccata
    17641 ataaaaatat aatcacaaag agcctctact tcttccatgt aatgggtcgt ataaataact
    17701 gtggcacctt ctttgtttag caaacgaatc gactctaaaa tatgattacg tgattgagga
    17761 tcaatcccta cagtcggttc gtcaaaaatg attaatttag gtgaatgaac tagcgcacaa
    17821 gcgatattta accgtctctt cattcctcct gagaattgac ttggaaactg cttagcttgg
    17881 gagtgtagcc ccacaaattc taaacttttt agaacttgtt ttttaagctg agctcccttt
    17941 aagccataaa gtgacccaaa tagttctaca ttttcataag cagttaggtc tggataaaca
    18001 gcaatgtctt gaggaacata acctatctgc gaacttattt ttcgaatagt cttttgggat
    18061 tgccctaaaa cagtaatttt accggaactc aaagggatca agcctaagat aagattaatc
    18121 aaggtagact taccagcacc atttggtcct aacagaccat aaatattacc tgcttcaata
    18181 gacaaggaaa catcattgac agctttcttg ccgtttttgt aggacttgac aacatttgtt
    18241 aattgtacaa aactcatata tcactccttt ttaatattgc taatactctt tgcaactaat
    18301 catcaaataa gtcaccgtta atatcatcgc acctactagc caaccatata ataaagttgc
    18361 cgtaactgcc catagtccaa ctatagtgaa aagacaaaaa gctaatagta catttaacca
    18421 gcctgaatag cgacaagcct tgactatccc ataacctaaa ataagcgttg ctaaacttgt
    18481 tagcgactgt tctcctaata atataagcaa gcccaaataa aagagactgt ctactagaat
    18541 agctatgcct agtaaaagta tacttaagac tggtttatct tgtctaaaag gcagccaagt
    18601 tattttttta aagagcaatc gactatcttt ctggagcaaa acatcaaaga taagaattaa
    18661 aactaaaagc gtcatctcta tgatggccat aagatacaac actaaataac aaagaatctg
    18721 ccaatgccag ctagtttgtg tcaaaaaggt cagaacctct cctagattaa tagtagctga
    18781 gtaaaagaat aaaacaagta taataatgac aaattgaaac agactcagta agacatctaa
    18841 aaaatcttga taggataagg ccactaacca ttgaagatgc catctagcca aacaacgtct
    18901 tcgataactg gtcaaccaaa gagtagttag acctaaaaac gacagcaaaa ccaatagcat
    18961 catgtcaccc ccttcttctt ttttgtttga agatggctaa gacactcaac tagcaagttc
    19021 cctcctacat gcattatcac aaccaaccac aattgactag tcagcatgta aatactacca
    19081 taaatgatgc ctgttaccaa cttagcataa aaaatggatt tccccatcaa taagtgattt
    19141 aaagcatagc aaaaactagt tacaattaaa acaataattg ttggtaaatg ccattgaaaa
    19201 gctaatatat taaaccacaa aaaacgataa atcgtctctt caaataaagc tagtaataaa
    19261 ggatagccaa tcttagacca atcatttgtc gttcctacaa acgagaaagt tgcaggaagc
    19321 cattgctttt tttgccaaca acgtaaacca tgtaagaaaa cgagttcaat cagtactatc
    19381 aagacgcaaa atacaagtcc cataaagaac gtcttactat caatactttc aagagacaag
    19441 gttatttgag gtgacaaaac ataaatactt accaaagcca aaatgtcata aatcagagtt
    19501 gctcttagaa aaccaattaa attaaatgca taaccactat cttcctttcc aaaaatagct
    19561 tgcataggaa ttgaaggaca aaaagttacc aacaaaaaac aaagattaag gcattggatg
    19621 gacaaaggca tttgttacac ctccaaactg tctcattcgt ggatgattag caaaaggaaa
    19681 tgctggtata cacatttcaa gaagttctgg catcagaact cgcgtgacgt accagccttt
    19741 ttctagagct tcaggaggtg taatatctaa aaagacagca ttaggactga ctttcttagc
    19801 ataagctaat aaagtcttca aatcttcttt tttatcttta ccagaatgat cttccaaatc
    19861 acttaacaga acttctcctg aaatcaaagg ttcaaaggcc ttccatttat ggtcttggtc
    19921 cttgggatgt gcataataga agacattact atctaaatct aaaaacaaag gttcttcgca
    19981 ttcaatattt gcaagggaag ctttttgata taaaagatta tagtagtaac tatagctaat
    20041 agctgaagct tccatgatcc ctcttagcaa agcatgttta ggatctaacc cagcctgcac
    20101 tccaaacaag aggtaaggcc cttcatcata tttatttttg agaatgattc caaacgtata
    20161 aagaggatta tcttctccta cagtcatatc aataggaata atgtcgtata gactgtcttt
    20221 tcccagtcta gcttcttcta aaatcacttc aatatcagga tcatcaacaa taatcttggg
    20281 gcaaggcttc ttagtatacc agctaagcat cattgaatcg atctggatgt attcaatcaa
    20341 actgttgcac atagccgctt ctaacgtttt gtgagaagct gttcccgtag aaaatcctgg
    20401 tattatccgt ctttctccta cagtctcatt tgttttatac ccaacacaaa gcatttgagc
    20461 tggaacatac atctctgcgt cctcaaagaa catgggacat ttaacccagc ccaatacatc
    20521 gttctcagtt accattttat cgcacatcat catttgcaag tcacaagata atgcgatctg
    20581 ttcttgagta aacacttgca agtattctaa aggcatcact ttatgtaaca gttttaactc
    20641 attataagaa gcatagacaa tccgatctga tagtaagtct ccggcaatta cagtagcata
    20701 gcgttcaata ctttctccta aatacttgat aagtgcttct tcatagtaac tgccgtaacc
    20761 aataatgtga taactaactt gactcaactc acctataaat tgtttatgat aatctggcat
    20821 ttggccagta atacttttta agtaaacatc gtgaggatgg ttgcaaactg gaacttgaga
    20881 ttgatttaag atacctgttc gattgccact taaacttttt aattcatcaa aaatatgatt
    20941 aaaagaagga taatagtata acataataga ttctccttat aggttactcg tcaaggagtt
    21001 ttttaacaat ctcacgtgtt gatatctgct gatcctcata ttttaatttt gccaaagctc
    21061 cttgtgtctg tgaattagac attttcaaaa tatcttggac ctgaatctca agcgtcggta
    21121 agtaaatgct taataaacgt ccctcaaatt tagaactacc tgtttgagca ataatgaaag
    21181 cttcgctaac gactaaattc attaaaacat ttaacaatgg taaataggcc tggctcacat
    21241 cttgtgttgc tggtaaaatc tgattagcaa aatgttgata gagagttgag tcttgtaaac
    21301 gtgccaaaac tcttctttct aagctatcaa agtcagcact atgaggagga ttcaaagtac
    21361 aagtgtgcaa gaatggtcca tctacaaaac caatcactaa ttgttttttc atagctacgc
    21421 taacttcatt caagtgtcgc aacatcataa tattcaagcg ttcttgacaa ataataatac
    21481 tttggtaatc gcacaaggct tctgataaac aattcatgtt acggcgatgc tccaaagcat
    21541 ctaaacgcga actaacatct gtctgctgaa ttaacatttt taagtcatcg ctagccactt
    21601 gcaattgtaa atgcagttgt tcagctagca actcagcaga ttcattaaca taagtcgaat
    21661 cactgataaa caaaacagga gcattgctta ccatatgacc tgcttgcgcc ataaaacgga
    21721 aattacccat taaaattttc atgacatttt cttcaagatt gtagtcatcc tcaagcatca
    21781 gcatatcatt gtaatacaag cctgaaatga cttccattaa attagcgaac atctcaggtt
    21841 ctaaagtatt ttcataagtt tcagtatcaa tggctactcc cttaaccaac tgtgaaacta
    21901 tttcttgata aactatcttt aaattttgag gttcttgtgt caaatctaga atggcttcat
    21961 taaaatccca gattcctttt ctaaaacaaa atgtatcttg aaatgtcacg acacgaacat
    22021 tactattaag ttgatatttc attgagactc cttagttcct acgagtgtca tgtgaatcac
    22081 atgctctcct agcccatcta aatctaataa ttcttggaga tattccttat tataaccacc
    22141 aatatcaata cttccataag ttaaggcagt tgcagtcaat tgaatattct gggctatttc
    22201 tcctgattca ataaaagcat aggcagtcgc ctgattccca tatttacgtg tatttttcat
    22261 gtaatggtag acataaataa taataatatt acaattttca gcattaatag caccgtattc
    22321 cgcaaaatct ctaacgtttt cctcagagct atgccgataa caccttagtg catgctgata
    22381 gggtagatat tcatagaaac catctattaa tttactgatg tttctagcat aaaaaactaa
    22441 atgaataggg tataaacctc cacctgaagc acagtttctg agtgtaatct tatctgatgc
    22501 tccatctcta attgatgctt gtgaactaac accacatgca taataaagaa tgtttgataa
    22561 atcttgaaga ggcatttgtc tatctgaaaa ttgacgatga cttcttcgtt ttataataca
    22621 tgtcgacaga gcagttgata tcttggtagg ttttggtaaa cgaattatct tttcctgact
    22681 ttcataaacg taactacttg aaaaattagc aacggcagaa tctgtaaaaa agtcgacaat
    22741 actagcttgg aagcctaaat aattattatt agctttatag ttcatcaaaa aatgttgact
    22801 aagattatcg gttgcattag gcattaaatg ttgagcaact agctgctttg acgtttttag
    22861 caccgtttga tgatggtaat ccgataagga caagtggttt gtattaaatt caaacaattg
    22921 ccttgctttt tcaacagtta ttggcggaca attctcttta ggttgttgtt cctttgtaaa
    22981 aaatgacata actatctttc ctatctcatt ttactaaaca tatagtaatt agcaggtact
    23041 atctagtacc tgctaattac ctgagacgtt agcatcaaga agattacttt tgtctcatgt
    23101 tttctaacac ctctatgcca ttcatctata atcagaagtt accaaagata acttagttgc
    23161 tagcaaactc attaccttaa tatcactatc acatagagat gctaaataga ttatttacct
    23221 ggcgtataac ttccgctacc accttgagaa ttaccacttc cagtagcaat tgagaagcaa
    23281 caagtagtac agcagcaaca gcagcctcca ggagcaactt gagttgtttc agctacacta
    23341 gtagctaaaa tatttgaagt aaattttaac ataaggttta cctccttatc taataagtaa
    23401 ctgataagaa cgcgagtcgt ttatttttaa ccttatttta aaaataaagt taacacaaca
    23461 aggacaaggc tagctcaatt gtactgttgt aacacaacaa ctatctagtt cttatcactt
    23521 ttattatagt aaaaaatgat taatatgtaa accctttctt tttaatatta tcaaaaatga
    23581 caaaaaaaat aataattgat taatatagaa acaattatga tctaatatac tgaattttat
    23641 aatagattct atcaataact atgtgtaaac aatcaagtag aaatgtcttt attaagcgaa
    23701 tcaatcaagg ttgtagagta aatactggac tagatttcat gtagaaatta gtaaaagtta
    23761 ggagattttt tggtcgtttc aaacgataac atcatcttct tataacaaga acttcactca
    23821 gagataaact attctaatac tttagtagcc tcttttgagc ttgtagcacg aaaattcgct
    23881 cgaactgatt taaaataggc attttatctg ctcgcttgat ccctactgcg cgaccaagaa
    23941 accttttatt cactagaaca attaaccctt aaaaccaagg aggaagtcca ctggtggaac
    24001 taccatcacc ttcacagtaa tcttaattat caaacaccca taactaaatg agtcatcgtt
    24061 tgaacaaaaa acgccttata aattctgtac agaaaactgt tgccttttca attgaacttt
    24121 gaaatgtttt caatacactc cgttcttggg cccttacgat ccaaaaaacc aaggtgattt
    24181 caaaatcacc ttggtttgaa aatatagtag aaagcaattt ggataaagcc aggttcttag
    24241 tctttacttt cttttttctt gctataaagc accataccta aaagaagaga cataactcct
    24301 aagccaacaa gaacaattga gtttgcatca cctgattttg gaagatggtt cgctgacgat
    24361 gattttgcga ctgccttatt aggcacatag gctgcacgga tagcagtaat acctttatct
    24421 attgcttctt tcacagacgc aatagattgt gctgaacgaa cagtcgcaat agccgtctta
    24481 agcgctaatg atacattttc tgattgttca gctttttgag cttcagtcaa tttagcatca
    24541 gttgtaatct tagcaagtgt ttctgccgca gcttgtttaa gagcttcaat agcagcttgt
    24601 ttcgcttcgt taagagcagc tgttgcttga tcagtatctt tagctaaacc agcaacatat
    24661 gctgaacgga tagctacagt accaaggttt tctgccaact taacagcttc aactgtctta
    24721 gcagcctcaa cttgaccaag tgctttcgca agagcttgct caacagcttg tttttggact
    24781 gctttttcag tttcgctcaa gtaacggtct cctgaaataa gagctttagt ttctttagct
    24841 actttttcaa gattagcttt agcagcttct ttatgtgctt caacaccttc acctggacgg
    24901 taagctttgt tgatagctgt gatgccttta ccaagttctt ggttaatgcc ttcagctgtt
    24961 gtcgctttat caaccgctgc gatagcagct ttaagagctt tagcaacagc ttctgtttgt
    25021 ttagctttat cagcttttgt taaggttggg tcgttagcga tagcttcacg aaccttagca
    25081 gcttctcttt caagatcagc cttagcggct tctttacgtg ctttaacacc ttcacctgga
    25141 cggtaagctt tattgatagc tgtgatgcct ttaccaagtt cttggttaat gccttcagct
    25201 gttgtcgctt tatcaaccgc tgcgatagca gctttaaggg ctttagcaac agcttctgtt
    25261 tgtttagctt tatcagcttt tgttaaggtt gggtcgttag taataagagc tttcacttta
    25321 gcagcttctt tttcaagatc agctttagcg gcttctttac gtgctttaac accttcacct
    25381 ggacggtaag ctttattgat agctgtgatg cctttaccaa gttcttggtt aatgccttca
    25441 gctgttgtcg ctttatcaac cgctacaatt gctgtattct tagctgcgtc aaccgctgct
    25501 gtttgtttgt ctttagcagc ttgagttaag gtcggatcct tagcaataag agctttcact
    25561 ttagcagctt ctttttcaag atcagcttta gcggcttctt tacgtgcttt aacagcttca
    25621 cctggacggt aagctttgtt gatagctgtg atgcctttac caagttcttg gttaacacca
    25681 tctgctgttg tcgctttatc aaccgctgca attgctgtgt tcttagctgc gtcaaccgct
    25741 gctgtttgct tgtctttatc agcttgagtc aaggttggat ccttagcaat aagagctttc
    25801 actttagcag cttctttttc aagatcgtct ttggcagtcg ctttacgaac atcaacgcca
    25861 tcgccatgtt ggtattggtt gttgatgttt tcaataccct taccaagagc atcattgata
    25921 gcatcagctg tttgagcatc atcaacagac ttgattgcgt ctaacttagc ttgttcaact
    25981 ttgttgattt gatcaacttt ttggtcatga gtcaaggttg gatcatcatt gattagtttc
    26041 gtcactttgt ctgcttcttt aagaagaaat tctttggcag cattacgacg atcattaagg
    26101 ttttgacctt ctttgtactg tgccaggatg tcacgaacac cgtcactata tgctttatca
    26161 acacctgcag catccttagc tgcatcaata ttctcgatac ctttagcata ctctgtatca
    26221 acacctaata actgttcttt acgttgatca tcagtaagcg ttttgtcttc aataatgaga
    26281 gcttttgtct tagcagcagc ttcagcaagg tctttcttag caaggtcttt acgagcttcg
    26341 atacctttac caataacgtg ggcattttcg atcgctgtga caccgtcacc gtaagctttg
    26401 tctgcttcat ctgcatcttt agcttcagca atcttatcag tcgcaagttc aagctctttt
    26461 tgaacctctg ctttttgttg ttctctcact tctggtgtta aggttggatc agcctcaatt
    26521 tcagcagtga cacgtttagc aacagcttca agagctttct tgtgctcatc tttacgaacg
    26581 tcaagacctt tacctgactt gtgttggttc ttgatgctgg ttacaccgtc accatacgct
    26641 ttgtctaaag catcagcatc tttagcctta gcaagttctt ctttagcttt agttgcttct
    26701 ttatcaacat tgccacgttg tgtttctttt tcagcttcag tcaaagtcgt atctgctgtg
    26761 atagcgtcct tagttgcttg cgccacttcg tcgattgact tgttgtgtaa tccacgacga
    26821 gcttcgatag ggtcacctga ctt
//
LOCUS       DAUVUY010000013        26380 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.013, whole genome shotgun sequence.
ACCESSION   DAUVUY010000013 DAUVUY010000000
VERSION     DAUVUY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26380
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.013"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            153..695
                     /locus_tag="VOX58_000536"
     CDS             153..695
                     /locus_tag="VOX58_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HES5877789.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            704..1987
                     /locus_tag="VOX58_000537"
     CDS             704..1987
                     /locus_tag="VOX58_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HES5877790.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCENSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2003..2863
                     /locus_tag="VOX58_000538"
     CDS             2003..2863
                     /locus_tag="VOX58_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HES5877791.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTRSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            2865..3830
                     /locus_tag="VOX58_000539"
     CDS             2865..3830
                     /locus_tag="VOX58_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HES5877792.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            3941..5224
                     /gene="aroA"
                     /locus_tag="VOX58_000540"
     CDS             3941..5224
                     /gene="aroA"
                     /locus_tag="VOX58_000540"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HES5877793.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            5217..5708
                     /locus_tag="VOX58_000541"
     CDS             5217..5708
                     /locus_tag="VOX58_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HES5877794.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFANDNSIIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            5916..7367
                     /locus_tag="VOX58_000542"
     CDS             5916..7367
                     /locus_tag="VOX58_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HES5877795.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDTPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            7449..9083
                     /gene="rlmD"
                     /locus_tag="VOX58_000543"
     CDS             7449..9083
                     /gene="rlmD"
                     /locus_tag="VOX58_000543"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HES5877796.1"
                     /translation="MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMR
                     VLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDS
                     LYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYY
                     IQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPK
                     VFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAI
                     SAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFAKTVKAVYGVEV
                     IEAAVRDAQHNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFI
                     QASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVETVALLSK
                     LGVDKHINIEIEMDELDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKRKCGIEL
                     REHYNKSKKEKQIIPQCTPEKEEAIMDALRHFKMIE"
     gene            9239..9775
                     /locus_tag="VOX58_000544"
     CDS             9239..9775
                     /locus_tag="VOX58_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HES5877797.1"
                     /translation="MRSYESKEELKNEIKKTFEKYISEFDNIPEELKDKRLEEVDRTP
                     AENLAYQVGWTTLVLKWEEDEKKGIDVKTPSDKFKWNQLGELYQWFTDTYAHKSLYEL
                     KEQLTENVKKIYLMIDELTDEELFKPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKLVLYIEKYK"
     gene            9795..10217
                     /locus_tag="VOX58_000545"
     CDS             9795..10217
                     /locus_tag="VOX58_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HES5877798.1"
                     /translation="MAVAKIKVTLNCPIEKVWDKVTDLRDFSWRSDIKDIKIIDDKNF
                     VEITKDGIETNFKVIKCTKHQCWSFEIENANIKGTWIGKFYSNGDKTTLDFTENIISK
                     KFIFKPFVGLYLRNQQKLYFKDLKEVLNCEEASHVQVL"
     gene            10285..10902
                     /locus_tag="VOX58_000546"
     CDS             10285..10902
                     /locus_tag="VOX58_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877799.1"
                     /translation="MIESRLKFDKITSFDEFNKYYWYREELSQICKLLGLEYRGTKQE
                     LTYIIEQYFKGDVIKKSSIKNIKKQVDYITLDMPLLECGFSFNSKFREYFSVVTGVLP
                     FKFTADMATAWRKVKRQNDLSFTIQDMLKVYYGKSDYAKYDNSVCQWNQFLKDFCADE
                     NSCNYSNKIQVAAILWKEVRDSKNKKVYSRKLIKKYEDKIEDYHK"
     gene            11007..11297
                     /locus_tag="VOX58_000547"
     CDS             11007..11297
                     /locus_tag="VOX58_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HES5877800.1"
                     /translation="MKKLEQIRKESKEIKNKIDDTEERLRQLKNQEKKILKQDIEKRR
                     KERTHRLITRGAILESLIENAEELTDEEIKILLEEAIKTKEFKETLKLMREN"
     gene            11765..12139
                     /locus_tag="VOX58_000548"
     CDS             11765..12139
                     /locus_tag="VOX58_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877801.1"
                     /translation="MSCIMSQMNTGKIVFPSILAMMLLQSIVASIASLIFPAGLIGAK
                     VTEKLYTGKSYIVFLVFSSIIPAIYFTAIMSISGLLRMKGYDENFWKIYFSSFPINVV
                     FGYFSSIFWNVILDKFLRRKEA"
     gene            12141..12566
                     /locus_tag="VOX58_000549"
     CDS             12141..12566
                     /locus_tag="VOX58_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HES5877802.1"
                     /translation="MNKSIGKLITYLSRCIQQELKNVVVPFDISVGEEPYYMALTKQD
                     GLTQDELTKLVKVDKAATTRMVKSLEQKGYITRETDPKDKRNKKIYLTERGRLKYEPL
                     SRALKEFNQKLTSEWTDEQYKIVYQSLEMLQKKFEIDNK"
     gene            12841..12939
                     /locus_tag="VOX58_000550"
                     /pseudo
     CDS             12841..12939
                     /locus_tag="VOX58_000550"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13062..13382
                     /locus_tag="VOX58_000551"
     CDS             13062..13382
                     /locus_tag="VOX58_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HES5877803.1"
                     /translation="MAIKRIKLSIEEQIEKKEESIKQLKNQKIQLKKKLNEQERKARN
                     KRLIEKGAVFESVFEESINLTIDEFYKLMKMLNDEEIRLNIMEILEERVDDDVEKSPK
                     DEIT"
     gene            13697..15337
                     /locus_tag="VOX58_000552"
     CDS             13697..15337
                     /locus_tag="VOX58_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HES5877804.1"
                     /translation="MTDSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVIHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERFIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKILNKNGKPKTRKVELTIWNDKGNVEKWRENFSDLCNEYLAKNKIEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNDLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDIERDKVIDNPKLFNLTNYLLTYSEIQKEKSKYLKGYAKT
                     NKEKYDFKKLTSAYSYLRKNNIETIGQLQTKIETLKSNSYKLNKKAKTIHKEMEDVEK
                     KILYYEIYKAKKGVYEEYQKKNIFTKDTFYNKHKKDIDQYKVVSGKLKKLLSDKEKLS
                     PKKWNEEKNLLMVNLEEINKEKDKIKDEYQEINHIKYSVDFVNKELGIDLSIEIDKLI
                     KQGEKPSVIAQIKKFQDQVNKDNEYRESMKNKKMDQER"
     gene            15411..15584
                     /locus_tag="VOX58_000553"
     CDS             15411..15584
                     /locus_tag="VOX58_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004829396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877805.1"
                     /translation="MNKRQELIDELIKADQDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHTNC"
     gene            15768..16295
                     /locus_tag="VOX58_000554"
     CDS             15768..16295
                     /locus_tag="VOX58_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HES5877806.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKAAKESINLEKEIRKKMNDTLE
                     KANGNLKSLMGDEIKIKDLYKKGQLENISIDQSNLKDLKKELNKLGVSFSVMKNKESQ
                     NYEVFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKNKTKEKVKRKV
                     KEKVKPNKKDMTREI"
     gene            16421..17149
                     /locus_tag="VOX58_000555"
     CDS             16421..17149
                     /locus_tag="VOX58_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HES5877807.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKS
                     TPIFYGEFKNVRLSKEEYKNLKEKLNSHTEIMINKLSRYMESSGKTYQNHYATILNWY
                     DKDKDKLRQKSLNKKMNYDVGESL"
     gene            17847..18008
                     /locus_tag="VOX58_000556"
     CDS             17847..18008
                     /locus_tag="VOX58_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877808.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     KAKTQIETD"
     gene            18105..19784
                     /locus_tag="VOX58_000557"
     CDS             18105..19784
                     /locus_tag="VOX58_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HES5877809.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRKQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGAGMFGNKCTKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFRKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKIIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLITAL
                     ISEIQVYEEKQPNGQWLKSITFKLPIIDDDLNISLDNNEQVECVSLLEKRS"
     gene            19936..20717
                     /locus_tag="VOX58_000558"
                     /pseudo
     CDS             19936..20717
                     /locus_tag="VOX58_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            20802..21203
                     /locus_tag="VOX58_000559"
     CDS             20802..21203
                     /locus_tag="VOX58_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HES5877810.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(21308..22792)
                     /locus_tag="VOX58_000560"
     CDS             complement(21308..22792)
                     /locus_tag="VOX58_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HES5877811.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLKKNGIASYLHYPIKSYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCISQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            23166..24386
                     /locus_tag="VOX58_000561"
     CDS             23166..24386
                     /locus_tag="VOX58_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HES5877812.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLSL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     SIGQLKAERYQQLR"
     gene            complement(24506..24871)
                     /locus_tag="VOX58_000562"
     CDS             complement(24506..24871)
                     /locus_tag="VOX58_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HES5877813.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            24986..25717
                     /locus_tag="VOX58_000563"
     CDS             24986..25717
                     /locus_tag="VOX58_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HES5877814.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVVGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQRTSLVKITIQEGKFHQVKRMVAACGKGVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
     gene            complement(25766..>26380)
                     /locus_tag="VOX58_000564"
     CDS             complement(25766..>26380)
                     /locus_tag="VOX58_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HES5877815.1"
                     /translation="VQRLMKVMSLAARIRRKRKYSSYKGEVGKKADNLIKRHFEGSKP
                     YEKCYTDVTELALPEGKLYLLPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKTFPADSY
                     SGTILHSDQGWQYQHQSYHDFLESKGILPSTSRKGNSPDNGMMDSFFGILKSEMFYGL
                     ETTYQSLDKLEEAITDYIFCYNNKRIKAKLKGFSPVQYRTKSFQ"
BASE COUNT         9540 a         4094 c         5230 g         7516 t
ORIGIN      
        1 tgaaagacta aatgttctca aactcattag aacaagcttt aataaaacta acccagttgt
       61 atcaaaggat agataaagac taagactgtt atagaatatt tgcaaaagat ttgttataat
      121 ggacccaata caggtattgt gaaaggtata atatggatat ttggacaaag cttgcagtgt
      181 ttgctttttt tgagactccg aaagtaatat tacggccttt tcgctatgaa gatcattggg
      241 atttttacag catggttaac gacactaaaa acctttatta tgtttttcca gaacaaaaaa
      301 ctaaggcagc aagtgactat cttttagtac atagttttat aaagtttcct ttaggtcagt
      361 gggcaataga agataaagca acccaccaag taataggttc tattagaatt gagcattatg
      421 atgctaaaac gcgttgtgct gatattggct attttttaaa ctatgccttt tggggacaag
      481 gaattatgac agaagtcgta ataaaacttg tttatttatc ttttcacgaa tttggtctca
      541 aaacgttgcg tattataact catttagaaa ataaggccag ccagaaagta gctaaaaaag
      601 caggttttca actaaaaacc tgttttaaag gaagtgatcg taatactcat aaaatctgca
      661 tttataaaat gtaccaacta actaatgata ggtgataatc attatgagca aacatcaaga
      721 tattttagat tatttagaaa aacttgctat tggtaaaaaa gtgagtgtta gaagcatttc
      781 aaaccactta aaagtaagcg atggaacagc ctaccgtgct attaaagagg cagaaaatcg
      841 aggtattgtc gaaaccaaac caagaagtgg aactgttcgt attgaaaaaa aaggacgggt
      901 tcgtattgac cgtctgacct attccgaaat tgctcggatc agtgattcgg aagtgctagc
      961 gggacatgct ggtttaggac atgaattcag taggttttca attggtgcta tgactcaaca
     1021 aaatattcgt cgttacttgg tcaaaggagg gttattaata gttggggatc gtgaaacgat
     1081 tcaactctta gctcttgaaa accataatgc cattttggtg acgggcggat ttccagtatc
     1141 caaacgtgtt attgaaatgg caaataacca acgaatccca gttatggtaa ctcattatga
     1201 tacctttact gtggcaacaa tgattaatca cgcgctatct aatattcgta ttaaaacaga
     1261 tctcaaaaca gttgaacagg taatgatacc tattacagat tatggttacc tttgcgaaaa
     1321 cagctctgtt gaagaattca atacattaat aaaaaaaaca cgacaagtca ggtttcctgt
     1381 attagattat aaaagaaaag tgattggtgt agttagtatg cgtgatgtgg tggatcagtt
     1441 gccaacaact aagcttacca aggtcatgtc taaaaatcca ataacggcta gaccaaatac
     1501 tagtcttgct aatattagcc agaaaatgat ctttgaagat ttaaacatgt tacctgtcac
     1561 tgacgaagaa aacaatttac ttggtatgat tactcgtaga caagcaatgg aaaatttgcc
     1621 aaatcaccaa cccaataacc cctatactta tagtgagcaa atactttcta accttgaaga
     1681 aacggttgat tattaccaag tagtagttga gccaacaatg attgatagtg ctggtaacat
     1741 gtcaaacggt gttatttcag agttcttaaa ggaaattagt atcagagccc tcacgaaaaa
     1801 gcaccaaaaa aatatcatta tcgaacaaat gatggtatac tttttacatg ctattcaaat
     1861 tgaggatgaa ttaaaaatat atcctaaaat cattactgaa aatagacgaa gcagcaccat
     1921 tgatattgaa atttttgtgg atgatcaagt gattgctaaa gctattatta caacgaaaat
     1981 aaattagagg agtaacaaaa caatgataac attaaaatca gcccgtgaaa tagaagcgat
     2041 ggatagagca ggagacttct tagcaggtat ccatattggc ttaagggata ttattaagcc
     2101 tggtgtggat atgtgggaag tggaagcata tgttcgtcgc cgttgtaaag aagacaacgt
     2161 tcttcctttg caaatagggg ttgatggtca catgatggac tatccttatg cgacctgttg
     2221 tggtctaaat gacgaagtag cacatgcctt tccccgtcat tacattctga aagaagggga
     2281 tttgcttaaa gttgacatgg tactcagtga gcctttggat aaatcaattg ttgatgtagc
     2341 agctttagat tttgataatg tgcctgagat gaagaaatgg acaagatctt atactggtgg
     2401 tttagctgac tcttgttggg cttatgctgt tggaacaccg tctgacgaaa taaagcaatt
     2461 aatggatgtc accaaagaag ccatgtaccg tggtattgaa aaagcagtaa ttggcaatcg
     2521 tattggtgat attggcgcag ctgttcaaga atacgctgaa agcttcggtt atggtgtcgt
     2581 gcgtgatctt gttggtcacg gtgttggtcc tacaatgcat gaagagccaa tggtaccaaa
     2641 ctatggaaca gcaggtcgtg gacttcgtct aaaagaaggc atggtcttga ctgttgagcc
     2701 aatgattaat acaggaactt gggaaattga taccgacatc aagacaggtt gggctcataa
     2761 aaccctagat ggtggtttat cttgccaata cgaacaccaa tttgtgatta ctaaagatgg
     2821 tcccgttatt ttaacaagtc aaggagaaga aagaacttac taaaatggca gaaaaaaaat
     2881 ggtttgataa ggtactgtcc aagtggcagt atgaacccat tcaagtcttt atgaggcatt
     2941 tacaaagtgc agaaatggat ttatctgcca ttgcggttgc gtattacctt atcttaacgg
     3001 cctttccatt aattgttatt gcagccaata tttttcctta cctcaatatt gatattgctg
     3061 atttgttacg tttaatgaag caaaatttgc ctaaagatat ttttagaccg gcttctgcaa
     3121 ttgtagaaaa tattttctct aaaccgtctg gaagtgtttt gggagtagcg actttgacag
     3181 gattatggac aatgtctcga agtttgacat cgctgcaaaa agctattaat aaagcctatg
     3241 gcgcttctca gcatcgagat ttctttatcg gacacttggt tggtttgttg accagtttaa
     3301 ttattttatt tttattagcc tttgccttaa tcttttcaac attttctaaa gctgctattc
     3361 aagtgctgga taagcattac catttgagtg ataatatcac aacgattttc ttactcctca
     3421 ttcaacctat tacagttttg attatttttg ttggcttgat gctactatat tttttattac
     3481 ccaatgttaa gattaaaaaa attcgctaca ttttaccagg aaccctattt acatcttttg
     3541 tgatgacatt tttgagtaat ctggttggca attacgttgt gtataatgtt gagcggatgg
     3601 tagatattaa aatgtttggt tcagttatga tttttatcat catgctatgg tttattttct
     3661 tagcaagaat cctgatttta ggagctatat ttaatgcgac ctatcaagaa atgtccttgg
     3721 ggaaattaga aggtcgtagt ggtgatatga tcgctatcct taaaaaaaca cttgggaatg
     3781 attcagatct atctccttca cagtctatcg aagatagtca cactgattga tacaaaaaag
     3841 ataataagtc gtggagtgag aaactatttt cttgcttcat tttttatgag gataaataga
     3901 aaatcattga tcttagcaaa tagaaaggca gataaaacgg atgaaattgc gaacaaatgc
     3961 aggaccatta cagggaacga ttcaagttcc aggagacaag tccattagtc accgggcagt
     4021 catattagga gctgttgcta aaggggaaac acgagtaaaa ggactcttaa aaggagaaga
     4081 cgtgctttcc acgattcaag cctttaggaa tttgggggtc cgtatcgaag aaaaagatga
     4141 ccagcttgtg atagaaggtc aaggttttca aggattgacc gctccttgtc agacacttaa
     4201 tatgggaaat tctggaacct ctatgcgcct cattgctggc ttattggcag gtcaaccttt
     4261 tagtgtcaaa atgattggcg atgaaagctt atccaaacgt cctatggatc ggattgttta
     4321 tccgttaaag caaatggggg ttgagatatc tggagaaaca gataggcaat ttcctccact
     4381 acaattgcaa ggtaatcgta atttacaacc aatcacatat actttgccta tctcttctgc
     4441 tcaggttaag tcagctattt tgttagcagc tttgcaagca aaaggaacaa cacaggttgt
     4501 tgaaaaagag attactcgaa accatacaga agaaatgatt caacaatttg gtggacgatt
     4561 gatagttgat ggtaaacgca ttactttggt gggaccccag cagctcactg ctcaagagat
     4621 aactgtcccc ggagatattt ctagtgcagc tttttggctt gtcgccggct taattattcc
     4681 tggttcggag ctccttttaa agaatgttgg agtcaatccg acacgaacag ggattcttga
     4741 agtggttgaa aaaatgggag cacagattgt ctatgaagat atgaataaga aagagcaagt
     4801 aactagtatt cgagtggtat attctcactt gaaaggaact atcatatcag gaggtcttat
     4861 ccctcgattg attgatgaac ttcccatcat tgcacttctt gcaacccaag cacaaggaac
     4921 aacctgtatt aaagatgctc aagaattacg agtcaaagaa acagatcgta ttcaagtagt
     4981 aactgatatt cttaacagta tgggagctaa tatcaaagcg acagcagatg gcatgattat
     5041 caaaggccct acggtactat atggggctaa cacaagtacc tatggagatc atcgtattgg
     5101 tatgatgaca gcaattgcag ccttgcttgt taaacaagga caggtccatt tggataaaga
     5161 agaagccatc atgacgtctt atccaacgtt tttcaaagat ttagaaaggc tgtgtcatga
     5221 ctaaagtgtt attgggattt atgggagtgg ggaaaacaac ggtttcgaaa cacctaagta
     5281 tgcactgtaa agatatggat gctattattg aagctaaaat cggtatgtct attgcagctt
     5341 tttttgaaca gcatggagaa atagcatttc gaacaattga aagtcaagtg ttaaaagatc
     5401 tattgtttgc aaatgataat agtatcatcg taacaggtgg cggtgtagtg gttttacaag
     5461 aaaatcgaca attactgaga aaaaatcacc agcataatat tttattggta gcttcttttg
     5521 aaactttgta ccagcgttta aaacatgata aaaagtctca aagacctcta tttcttaagt
     5581 actctaagga agcattttat gaattttatc aacaacgtat ggtattttat gagggacttt
     5641 cagatttagt aatcagagtt gaccatcgta cgcctgaaga agttgccaac atcattgagg
     5701 gttattagta ggtcattgtc gtaatctgat atttatggtt ttaaacaggt atctgtctaa
     5761 agcacttctt atggcaagtt ggctttatta aatattagga aataatcttg attattctat
     5821 aatgattaaa attttgagag gacaatctgg ctaatatagg ttataatggt agtattcgtt
     5881 aaaagtgcaa ttgtaatact aagagatgat aagatatgac aaaatacccc atgggtggcc
     5941 ttagtcacca tgaagaatta cgttattttt atctcctaag gaatttgagt taccttagtg
     6001 agaatgaaaa aaaagagttt gcttttttaa aatctaaatt ggaaattggt cgagcttatg
     6061 ctccttctaa gcagcattat aggaagtcaa aacggcaaga accttacttt gaagatgact
     6121 actataacga ctactcacca aatgatttac tagaagatga tgacgtcaat catgactctt
     6181 cttttgttcc atatggttta ccgatatatc ctaaagaaga cagatattta aacaaaaaaa
     6241 caaaacttac agctcgtaga cctatagata ctccgcagcc tattgatgaa gacgatgctt
     6301 ttttaacaga gtctgttgct cgttgtgctc ttcctagatc tcaaaaaaga aagcataaga
     6361 aaaaaggatg tatgaaatgg ttttttaaca ttcttggctt attgctaatg actgtgctta
     6421 tggggttagg gttgatgttt gcaaaagggg tttttgatat ttctactaat aaagctaatt
     6481 acaaaccagc tgtttcccaa gcttttgatg gtcaagaaac tcaagatggt acgaacattc
     6541 tggttttggg ttctgaccaa cgagttactc aaggttctac agatgctcgc acagatacca
     6601 ttatggttgt caatgtcggc aaccatgcta aaaaaataaa aatggtctct tttatgcgtg
     6661 acactttgat taacattcca ggatacagtt ataatgataa ttcgtatgat cttaaattaa
     6721 atagtgcatt taatcttgga gagcaagaag atcaccacgg agcagagtac gttcgacggg
     6781 ccttaaaaca taattttgat atcgacatta agtattatgt gatggtagat tttgaaacat
     6841 ttgcagaagc tattgacacc ttgtttccaa atggtgttaa gattgatgcc aaatttgcga
     6901 cagtaggtgg tgtagctgtt gactccgtag aagttccaga tgacctgcga atgaaaaatg
     6961 gggttgttcc caaccaaacc atcgaagttg gagaacaacg catggatggt agaaccttgc
     7021 tcaactatgc tcgtttccgt aaagatgacg aaggagattt cggacgaacc gtccgtcagc
     7081 aacaggttat gtcagctgtt atgtcccaaa tcaaagaccc aactaaactc tttacagggt
     7141 ctgcggctat tggtaaaatc tatgcgctga cttcgaccaa tgtttctttt ccatttgttg
     7201 ttaaaaatgg tgtttctgtt ttaggaagtg ggaaaaatgg agttgagcac gtgaccatcc
     7261 cagaaaacgg ggattgggtt gacgaatacg atatgtatgg aggacaagcc ctttacatag
     7321 attttgataa gtatcagaaa acgttagcca aattaggtct gcgctaagat atggtataat
     7381 ggagagtaac taaagttgct cttctttttt gagcaatggt tagccctaga aaaggaaaaa
     7441 gaataagaat gttgaaaaaa aatgatatta tccaagttgc tatttcagat ttaagccatg
     7501 agggagcagg tgtcgctaaa cacgatggtt ttgttttctt tgttgataat gccttgccag
     7561 aagaagtgat tgacatgcgt gtcttaaagg tcaataaaaa cagcggtttt ggcaaagtag
     7621 aagcctatca ttacctgtcg ccagctagaa atgcagatgt caatttaact tatttgcgaa
     7681 caggcattgc agaccttgga catctaactt atgaagacca actgactttc aaaaagaaac
     7741 aagttcagga cagcctctat aaaattgctg gaatcagtga cgtgacagtc gaaagcacga
     7801 ttgggatgac agaaccttta gcttaccgaa ataaggctca ggttcctgtt agacgtgtta
     7861 atggtcagct agaaacaggc tttttccgca agcattctca tgacttgatt cctatttcgg
     7921 attattatat tcaggacaaa gagattgatc gtctcattaa ttttacccgt gatttattac
     7981 gccgctttga tattaaaccg tatgacgaaa cagaacaaac aggtctcttg agaaatattg
     8041 ttgttcgccg aggccattac tctggagaaa tgatgctggt cttagtgact acacgcccaa
     8101 aagttttccg tgttgaccaa gtcattgaaa aaatcgttga agcttttcca gcagtggtgt
     8161 ctattattca aaatattaat gataagaaca ccaatgcaat ctttggtaaa gatttcaaaa
     8221 cgctttacgg caaggatacc attactgata gcatgcttgg aaacaactat gccatttctg
     8281 cccagtcttt ctaccaagtg aatacggtta tggcagaaaa actctatcaa acagctatag
     8341 ccttttcaga cctttctaaa gatgacattg tgattgacgc ctactcaggt atcggaacca
     8401 ttggcctatc atttgctaaa acagttaaag ctgtttatgg tgttgaagtg attgaagcag
     8461 ctgttagaga tgctcaacac aatgccgctc ttaatggcat caccaatgcc tactttgtcg
     8521 cagataccgc agaacatgcc atggcaacat gggcgaaaga tggcattaaa ccaagtgtta
     8581 ttctggtaga cccgccacga aaaggcttaa cagaaagttt catccaagcc agcgtggcaa
     8641 tgggaccaca aaaaattacc tacgtttcct gcaacccagc caccatggcg cgtgacatca
     8701 agcgttatca agagttgggt tacaaactgg ccaaagtaca gcccgtagat ttgtttcccc
     8761 agacgcatca cgtggagacg gtagcacttt tgtccaaact tggtgtcgat aagcatataa
     8821 atattgaaat tgagatggat gagcttgatt tgacaagtgc tgaaagtaag gcaacatatg
     8881 ctcaaatcaa agaatatgtt tggaataaat ttgaattaaa agtttcgaca ttatatattg
     8941 cacagataaa aaggaaatgt ggaatagaat tacgagaaca ttacaacaag tctaaaaagg
     9001 agaaacaaat tattccacag tgtacacctg aaaaagaaga agccatcatg gatgctttga
     9061 gacatttcaa aatgattgaa tagaaaagaa tgacagcata tcactttctg catttattac
     9121 attcctattt ggtataagaa caactattat tcttttcttg caaggtatca attagaaaat
     9181 agggtcaata taaagaatgg taggataata cctatatata aaccatggag gtaggcatat
     9241 gaggtcttat gaaagtaagg aagaattaaa gaatgagata aagaaaactt ttgaaaaata
     9301 tatttcagag tttgacaata taccagaaga actaaaagat aaaagactag aagaagttga
     9361 taggacacca gcagaaaatc ttgcctatca agtaggctgg acgactttag tattaaaatg
     9421 ggaagaagat gagaaaaaag gtatagatgt taaaacacca tcagataaat tcaagtggaa
     9481 tcaacttgga gaattatatc aatggtttac agatacctat gcccataaat ctctatatga
     9541 actaaaagaa caactaacag aaaatgtaaa aaaaatttat cttatgatag atgaattaac
     9601 agatgaagaa ttatttaaac cacatatgag aaaatgggca gatgaagcaa ccaaaactgc
     9661 tacttgggaa gtatataaat ttattcatgt aaatacagta gcgccatttg gaacatttag
     9721 gactaagatt aggaaatgga agaaattagt tctatatata gaaaagtata aataagttta
     9781 cttaggagga ttttatggca gttgcaaaga tcaaagttac attaaattgc ccaatagaaa
     9841 aagtatggga taaagttact gaccttcgtg attttagttg gagaagtgat atcaaagata
     9901 tcaaaattat tgatgataag aattttgtag agattacaaa agatgggata gaaacaaatt
     9961 ttaaagttat aaaatgtacg aaacatcaat gctggtcttt tgaaattgaa aacgcaaata
    10021 taaaaggaac ttggatagga aaattttatt cgaatggaga taaaacaaca ttagatttta
    10081 cagaaaatat tatttctaaa aaatttatat ttaaaccttt tgtaggacta tatttaagaa
    10141 atcaacaaaa actgtatttt aaagatttga aggaagtact taattgtgaa gaagcaagtc
    10201 atgttcaagt gttatgaaaa taatgaaata gataaattgg agtagcctat tcatatattt
    10261 agatcttttc gaaaggataa aacaatgata gaaagtagac ttaagtttga taaaatcaca
    10321 tcgtttgatg agtttaataa atactattgg tatcgtgaag aactttcaca gatatgcaag
    10381 ttattaggat tagaatatag aggtacaaaa caagaactca cttatattat tgagcagtac
    10441 tttaaggggg atgtgattaa aaagtcatca ataaaaaaca taaagaaaca agttgattat
    10501 attactttag atatgccttt acttgagtgt ggattttcct ttaattcaaa attcagagaa
    10561 tatttttctg ttgtaacagg tgttttgcct tttaaattta ctgcagatat ggcaacggct
    10621 tggagaaaag taaaaagaca aaatgatttg agttttacaa ttcaagatat gctcaaagtt
    10681 tattatggaa aatcagatta tgctaagtat gataattctg tttgtcaatg gaatcaattt
    10741 ttaaaggatt tttgtgcaga cgaaaatagc tgtaactact ctaataaaat acaagtagct
    10801 gcaattcttt ggaaagaagt cagagattca aaaaacaaaa aagtttattc acgaaaactt
    10861 ataaaaaaat atgaggataa aatagaagac tatcacaagt aaacagaaac tatttttttg
    10921 aatgaaaaaa attaattcag atagatgtta ggcgatagaa attaaaaact ctatcgtctt
    10981 tttttattac aagaaaggag ttttgcatga aaaaattaga acaaatcaga aaagaatcaa
    11041 aagaaataaa aaataaaatt gacgatacag aagaaagatt aaggcaacta aaaaatcaag
    11101 aaaaaaagat attaaaacaa gacatagaaa aaagaagaaa agaaagaaca cataggctca
    11161 taacaagagg agcaatctta gaaagcctta tagaaaatgc agaagaacta acagatgaag
    11221 aaataaaaat cctactagaa gaagcaataa agacaaaaga atttaaagaa acactaaaac
    11281 taatgagaga aaattaataa aaataaaggg tgttgtaaat aatcaaatct cccttagatt
    11341 ttctaagggc gcaattatac agctatcaga gattgatgca ttgcgttctc cgaaggctcc
    11401 taatcggagg gctgtgattt gtgcgatcaa tgagccaaac ggatatactt gcatattcac
    11461 tcgccgaatg ccccaacagc cgacagaatg tcggcttccg cagtcatggt ctgctccaaa
    11521 tcaaacacgg acgctacgct tcccctgcgc tggctgccgc cagccaccct ttgaaaggaa
    11581 aaatttttat aaacagtatt tactacgaag tttaccaata aaatattatt ttaagagatc
    11641 taagcttaaa aacaaattag ttgacgcatc aactaatttg tgatatactt aagttattct
    11701 taaaagtgag gtaagagtaa tgaaatataa acgttatatt ttccctatat tgatggggag
    11761 tacaatgagt tgtattatgt ctcagatgaa tactggaaaa attgtttttc cgtctatact
    11821 tgcaatgatg ttattacaat ctatagttgc aagtatagca tctttgattt ttccggctgg
    11881 acttataggt gcaaaagtaa cagagaaact atatactgga aaatcatata ttgttttttt
    11941 agttttttca tcaataattc cagcgatata ttttactgca attatgtcta taagcggatt
    12001 gttaagaatg aagggatatg atgagaattt ctggaaaata tatttttcct catttcctat
    12061 taatgttgta tttggatatt tttctagtat tttttggaat gtgatattgg acaaattttt
    12121 aagaagaaaa gaggcataaa gtgaacaaaa gcattggaaa acttataact tatttatcaa
    12181 gatgtattca gcaagaatta aaaaatgtag ttgttccatt tgatataagt gtcggagaag
    12241 agccttatta catggcactt acaaaacagg atggattgac tcaggatgaa ttaacaaaac
    12301 ttgtgaaagt agataaggct gctacgactc gaatggttaa atcattagag caaaagggat
    12361 atatcacacg agaaacagac cctaaagata agagaaacaa aaaaatatat cttacagaaa
    12421 ggggacgatt aaaatatgaa cctttaagta gagcattaaa agaatttaat cagaagttaa
    12481 catcagaatg gacagatgaa caatataaaa tcgtttatca aagtctggaa atgctacaaa
    12541 aaaaatttga gattgataat aaataagttt taatttattg cccctctaca catcgggtca
    12601 acttgccacg aactttcaca actaaatacc caccgctaaa acagcggcac accaagcagg
    12661 aaatctgaaa aaggtctcct ggtatggata caataaataa taagtaatca taggaggatt
    12721 ttaaattagc tagacactac gaagaagact tcaaaaggca aatagtagaa atctttaatc
    12781 aaggtaacaa cacatataaa aaattgggag aagaatacga tatagcaccg tcaataatca
    12841 gagtatgttt attggaacaa taacctaagg gttaatggat cattaggtta cttgacacca
    12901 gttaaatata ggcaatcaag attaactagt agttattagt taatctaaaa ttagtacgtt
    12961 cgattttaga ttgtacaaat aggtgttgac aatccacact aagctgaaaa acgattagaa
    13021 ataaaaatct attcgttttt ttattttaag aaatgataca aatggcaatt aaaagaataa
    13081 agttaagtat tgaagaacag atcgaaaaga aagaagaaag cattaagcaa ttaaaaaatc
    13141 aaaaaataca gctgaagaaa aaacttaatg agcaagaaag aaaagcaaga aacaaaagac
    13201 ttatagaaaa aggtgcagtg tttgaaagtg tttttgaaga aagtattaat ttaacaatag
    13261 atgaatttta taagttaatg aaaatgttaa atgatgaaga aataagatta aacataatgg
    13321 aaattttaga agaaagagta gatgatgatg tagaaaaatc acctaaagat gaaataacat
    13381 aattgtccct tggttacaag ggcgcaatta tacaccctaa agggtgcttg cgtgccttta
    13441 gagcaaaacc cttgcaggga gcaacaacca ttcgtttttt aaaagtcaag ggtaaataaa
    13501 ctcgctaagg ctcgccattg acttttaaaa tttgaaatga acaaaacaat taagccgaca
    13561 tactgaaaca acaaaaatta attcagtagt cggctttttc tattctatca tttcaaaacg
    13621 ctcattcaca aagaggatat aaaaatctat taagcctcca cctaaaacaa caaaaaaaga
    13681 aaggaagtga taaaaaatga cagacagttt tcatttttca gtaaacataa tatcaagagg
    13741 aaaagggaaa agtgcagtag caagtgcagc atatataagt ggagaaaaaa taaaaaatga
    13801 atgggacgga gtaatccatg actacacaag aaaagaaaaa atattagtaa aaaatataat
    13861 attgcctgat catataccaa aagaatttaa tgataggtct acattatgga ataaagtaga
    13921 aatggcagaa aaaaattcta atgcacaact agcaagacaa ttcataatag gactaccaaa
    13981 agaattatct ttaagtgaaa ataaaaacct agtcgaaaga tttataaaag aaaatctaac
    14041 atcacaagga atgatagtag attatgcaat ccatgatgag agtcaagaca aaaatggaaa
    14101 tatccattgt catataatga ccataatgcg accaataaac gaaaaaggag agttcttagc
    14161 aaagtcaaaa aaagaatata tcctagatga aaaaggagaa aagattttaa acaaaaatgg
    14221 aaaacctaaa acaagaaaag tagaactaac aatctggaat gataagggca atgtagaaaa
    14281 atggagagaa aatttttcag acctttgcaa tgaatatcta gcaaaaaaca agatagaaaa
    14341 aagagtagac cataggagtt ttaaaagaca aaattctgat tatttaccga caatccattt
    14401 aggatcagca gcaagtgcaa tggaaagaaa gggaatagaa actgacaaag gaaactataa
    14461 tagagaaata agaaaatata acgaccttgt aaagacaata aaagaagaga taaaaacatt
    14521 aaagggttgg ataggaaatc tattagataa cctatctact gcttatgaaa aatttaagga
    14581 tatagaaaga gataaggtaa tagacaaccc taaacttttc aatctaacaa attatctatt
    14641 aacttattca gaaattcaaa aagaaaaaag taaatatcta aaaggatatg ccaaaactaa
    14701 taaagaaaaa tatgacttca aaaagctaac aagtgcatat agttatttaa gaaaaaataa
    14761 catagaaacc ataggtcagt tacaaacaaa aatagaaaca ttaaagtcta atagttacaa
    14821 actaaataaa aaagcaaaga caatccataa agaaatggaa gatgtagaaa agaaaattct
    14881 atactatgaa atctacaaag ccaaaaaagg agtttatgaa gaatatcaaa agaaaaatat
    14941 attcacaaaa gacacctttt ataataaaca taagaaagac atagaccaat ataaagtagt
    15001 aagtgggaaa ttaaaaaaac tcctatcaga taaggaaaaa ctaagtccta aaaaatggaa
    15061 tgaagaaaaa aatcttttaa tggtaaatct tgaagaaata aataaagaaa aagacaagat
    15121 aaaagatgaa taccaggaaa ttaatcatat aaaatattca gtagattttg taaataaaga
    15181 acttggcata gacttatcca tagaaataga taagctaata aaacaaggag aaaaaccaag
    15241 tgtaatagca cagattaaaa aattccaaga ccaagtcaat aaagataatg aatacagaga
    15301 aagtatgaaa aacaagaaaa tggatcaaga aagataagac ctggtaaaaa tatcttatcc
    15361 aagctataat atgaccaaga aaataaagac cgttctagga ggtaatagag atgaataaaa
    15421 ggcaagagct aatagatgaa ctcataaaag cagatcaaga tggaacatac aaaacctaca
    15481 aaagcacaga agaaataaaa gcaatgaaca atgaagaaat acagattata tattccaaca
    15541 tgaaaaacta tctatcagac aaaagaacac acacaaactg ctgaaggtgg aaaggtattg
    15601 taagctattt aaaagtataa aaggtacaaa cacctaaaca agatatgaaa acatcaaaat
    15661 aaagcattcc accaccaaaa acaataaatt aaaaacaagc aaagggaagt atagaaaaat
    15721 taaaacctat acttcccttt ttttaattca aacaaaggag agaaaacatg ataaacgaag
    15781 aaataagcaa agaagcaggt caagcagcac aaaccataat atcatacaca ataaaggcag
    15841 caaaagaatc aatcaactta gaaaaagaaa taagaaaaaa gatgaatgat actttagaaa
    15901 aagcaaatgg aaacctaaaa agtcttatgg gcgatgaaat aaaaataaaa gacctctaca
    15961 aaaaaggaca actagaaaac ataagcatag atcaaagcaa cctcaaagac ttaaaaaaag
    16021 aactaaacaa acttggagta agcttttcag taatgaaaaa caaagaaagc caaaactatg
    16081 aagtattttt ccaagccaaa gacataaaag taatggaata tgcctttaag caagtcatag
    16141 ccaaagaaaa taaaaaagaa aaagaaagta tcctaaaaca aataaagaaa tacaaagacc
    16201 tatccaagaa caagaataag acgaaagaga aagtaaaaag gaaagtaaaa gaaaaagtaa
    16261 aaccaaacaa aaaagatatg accagagaaa tctaagggag taacaaaaag ttacaccctt
    16321 aaataaccat aataacaaga cttacgaaaa gcagaagtcc agaattccga aaatcggaaa
    16381 tcaagaattt cgattatcga aaatcaagaa aggagcagat atggcaacaa acaaaagata
    16441 ttattggata aaactaaaag aagaattttt cacagacaaa aggataaaaa gactaaggag
    16501 aatatcggga ggagatacct acacgataat ctacctaaaa cttttattac taagtctaaa
    16561 agatgagggc aaactctatt atgacggagt agaaagtgat tttataaaag agttagcact
    16621 aaccatagat gaaacagacg atgatgtaat ggtcacagta aattatttaa tcaatcaagg
    16681 cttactagaa gttgtaacag aaaatgatga atactattta actgaaatac caaacttaat
    16741 cggatcagaa acagcatggg cagagaaaaa aagaagatac agacaaaata aacagaggac
    16801 attgtcctta atgagtccaa cccatgtccg acaagagata gagatagaga aagatataga
    16861 gatagataaa gagagagagg gagagataga taaaaagtcc acccccattt tttatggaga
    16921 attcaaaaat gtaaggttaa gcaaagaaga atataaaaat cttaaagaaa aactaaactc
    16981 acacacagaa ataatgataa ataaactatc aagatacatg gaaagcagtg gtaagaccta
    17041 tcaaaaccac tatgcgacaa tcctaaattg gtatgacaaa gataaagaca aactaagaca
    17101 gaaaagttta aataaaaaaa tgaattatga tgtaggagaa tctttataga taaaaagagg
    17161 tggaaagcca ctattaaaga ctttaagtct aaaaaggtat caaaagtatg ccagagataa
    17221 taaaaatgta aatatgacac ttaaaaggcg attagaaaat taaagaaatg ctagaagatt
    17281 tttagatgac agttaccaag agggtctaat tttcactgct actcatgcag tgggaattgg
    17341 acctttttac ataagtaaat tctattcaaa attagttacc aagaaagact tcttttcact
    17401 gttaagtata agagggtaat ttatagaatt ttgttacatc aagataaatt ttctctgatg
    17461 gtcaaatttt aaaaagatta ataactctcg ataaattttg aaccttaaca attgaataga
    17521 ccaagacaag acgttaatta tttttggagc gtaataacct atccgtcaag atctttctgc
    17581 tgaataattc ggcaaaacaa gtactgctaa gcaaaaaaag gaagaagaaa gagagcgata
    17641 agtaagagtt tgaatatagg gagggatacc ctatccgcta tgaagaaaat gaggataatg
    17701 atacttctaa ggttgaagcc ggctcatccg gaatagaggc ggaggtgaga ttcctatgtt
    17761 gctaatccaa agcacttgtc aatgtcgtaa aacttaatta aacaaataat tattagatat
    17821 aaaactttat agagaggagg tatatattgg acaatgactg gaatggattg gctgatttaa
    17881 tagcaaatct gattgcaaaa tatgctggtg ctttagattt agataatctg cccgatccaa
    17941 cgccagcaaa aaatcaagag atgaaaaata gttttgacaa ggcaaaaaca caaattgaga
    18001 cggactaata aaagtgttat aatatacttg aaatgatagt ccaaacttaa attcaaaaat
    18061 caaagtttat atatgatata gaaagttata atgaggttaa tgctatgtca aaagaaaaaa
    18121 taaaagtata cctctataca cgagtatcta cgtcaataca gatagagggg tattcgttag
    18181 aggcacaaaa atcacgaatg aaagcttttg ctatttacaa cgattatgaa attgtcggag
    18241 agtatgaaga tgccgggaag tctggcaagt ctattgaagg tagaaaacag ttcaatcgaa
    18301 tgatggaaga tataaaatct ggaaaagatg gagtgtcttt tgttcttgtg tttaagttat
    18361 caagatttgc aagaaatgcc gctgatgttc tttctacact tcagataatg caggattatg
    18421 gagttaatct tatctgcgtt gaagatggca ttgattcatc taaagatgct gggaaactaa
    18481 tgatttctgt tttatcagct gtggctgaaa tagaaagaga aaacatccgt attcaaacaa
    18541 tggaaggtcg cattcaaaaa gcaagggaag gaaaatggaa tggtggcttt gctccgtatg
    18601 gatataaact tgaagatggc aagctgttta taaatgagga agaggcagtt gccataagaa
    18661 cgattttcga ccagtatgta aatactacga taggagccaa tgggctatct aaatacttag
    18721 agaatcatgg aattagaaaa atcccaagac agaatggaaa gaatcctttg tttgatgcag
    18781 gacttataag aaagatatta aagaatcctg tatataatgg gaaaatagca tttggaagaa
    18841 gaactttaga aaaagttcat ggtacaagaa atgaatataa gcaggttgaa caagatgaat
    18901 atctaatatc tgaaggtata catgaagcta tagtttccga cgaagtttgg caagctgctc
    18961 aggttaagct aaaatctcaa gcaaagaaat atgagcatgt gaataaagga aaagatacac
    19021 gcacgcactt gctttctgga attgtaaaat gccctatatg tggagcggga atgtttggaa
    19081 acaagtgtac caagaaaaag aaagatggca caaagtataa agatttttat tactatggtt
    19141 gtaaacatag gcagatgata agaggtcata agtgtacatt ccgtaagcaa attagagaag
    19201 aattgttaga tgatgctgtt gcagaggtga ttgtcaagat tgtaagtaat ccgaaatttg
    19261 cttctatgat gcaagaaaaa atcaacatga aggtggacac ctctgaaata gaaaaagaaa
    19321 tagataatta ccaaaaggaa ttgaggaaga gtcattctac aaagtttaag ataattgagg
    19381 aaatagataa tttagatgtt gaagataagc actataagag aaggaaacag gatttagatg
    19441 atagactata tcgtatgtat gacaaaatag atgaattaga atcatcacta attgatgcga
    19501 aagcaaagaa acagactatt gaagctgaaa aactcacagg agataacata tataaggttc
    19561 tgatctattt tgataaactc tataaagtca tgaatgatgt agagcgtaga cagttaatta
    19621 cagctttgat ttctgaaatt caagtttatg aagaaaaaca acctaacgga caatggctaa
    19681 aatcaattac tttcaagctc ccaattatag acgatgactt gaatataagt ttggataata
    19741 atgaacaagt tgagtgtgta agtttacttg agaaacggag ttaaagagtc cgcctcactc
    19801 tgttatttca aggtgagacg ctcaaaaggt ccactggacc ttttgactcc cgcagaccca
    19861 tcacgtcgag tgcgtagtgt tgctaatcaa ggagtaaggc agctgcctcc acatgacaaa
    19921 aataaggaga aacgaatgcc tattaccaaa cacgagattc cccttttaga atgtgatagt
    19981 aatccatcag ccgtcattat acctacacat gaaggtctac agctaagtgt gtctatgctt
    20041 tcttagaaga cgagattgaa gcttttgctt tagaccatca aggtaaaaaa gtagcagaat
    20101 tcatttcagc cacaaaggtt taccctgttt atcagatacc ttataaaggc caagacattt
    20161 gcctagctca agctcccgtt ggctcagctc ctgcggcgca attcatggac tggctgattg
    20221 gttatggggt taaagaaatg atcagtacag gaacctgtgg cgttttggtt cctatcgcag
    20281 aaaaccgttt tttagtgcca gttaaggctt tgcgagatga aggaacaagt tatcattacg
    20341 tggcaccatc ttgttatatt gatattgatc ctaagatgct tagaccgata gagaaaacgc
    20401 tttcagcaca ggggttagct tatcaagagg tcatcacctg gtcaacagat ggtttttacc
    20461 gagaaaccaa agaaaaagtg gcttatcgcc aggaagaagg gtgttcggtt gttgagatgg
    20521 gatgttctgc cttagcagca gtagctcaat taagaggcat cctttgggga caactcttat
    20581 ttacagcaga tactctagca gatgtagagg tttacgatca acgcaaccgg ggcgctgatt
    20641 cctttagttt tgcgcttcat ttatgcttgg aagtgttcaa tacgctagaa aaggatggaa
    20701 aggctactga tttttgaaac atgagctctg ccttctgttc ctatttgtag tataatgagg
    20761 cttgtattgc ttttagacta agcttagaaa agagaaaaac aatgtcagat ttaacacaag
    20821 agatgacctt aaatgttatt agtatttttg ataattacca aattgaactt gctgaaaaag
    20881 gacaccttat tttaagtact gaagtgactg agacggcttt gaattactat gggaatgctc
    20941 atggtggtta tcttttcact ctatgtgatc aagtaggtgg tttggttgct agaactactg
    21001 gcgtagaatc tgtcacctta caggccaatg ctaactattt aaaagcaggg cacaagggag
    21061 acaagctgat ggttgaaggt cgattggttc atggaggtcg aaccacgcag gtggttgatg
    21121 tgacgattca taaccaaaca ggagctcttc taacaaaaac aagcttgacc atgtttgtga
    21181 ctggccgacg tcataacggg tgattaatga atcaataaag gaaaaaagtc ccaactctct
    21241 gagaattggg acttttttcc tttattgaga gataccataa atcacccgtt agacgggtgg
    21301 ttatagttta tttacggtaa agtttgtgat gttggtaaac agggtcaaaa gtaatattta
    21361 ccccaagttt tcggagtaca tttttatctt cttcggttaa gataacggta gaatgggctt
    21421 cgctaccttt caaattgccg agttctttca tggctagatt agcttcttca ctagtcatag
    21481 cagtaatggc taacgcaatc agaatttcat tggaatgaag ccgcggattg tgactgccta
    21541 agtggttcac tttgagccct tgaataggtt tcacgtattc gggttcaatc aaatgagtgg
    21601 ttttatcaat ctttgccaag gttttaatcg cattgatgat aacagctgca gttggtccaa
    21661 agagttcaga tgttttacct gtcacaattt gcccatttgg tagttggaga gcaagggcag
    21721 gttggcctgt ttgctctgct ttttgatgag cagctacagt aacgtgacga tcgtcagggg
    21781 tgacaccgat atcattcatc aagagctcaa tttttttgac agcagattct gtgacccgct
    21841 ctgccttaaa atccacgagg gtttggtaat aacgacggat gatttcttgt ttagaagctt
    21901 cgatagcagc ttcttcattg acgatagaaa aaccaaccat gttaacaccc atatcggttg
    21961 gtgaggcata aggagacttg ctcaaaatac gctcgaaggt ccgatttaag acaggaaaga
    22021 cctcgatatc acggttgtaa ttaacggccg ttttgccata agtttccaaa tggaaggggt
    22081 caatcatatt cacatcatct aggtctgccg tggctgcctc ataagccaga ttgacagggt
    22141 gatgaagagg aagattccaa actgggaagg tttcaaattt agcatagcca gacttaaccc
    22201 cattgatttg gtcgtgatac atttgggata tacaagtggc taattttcca gacccagggc
    22261 ctggtgctgt aacaacgata aggttacgac tcgttttgat gtaatcattt ttccccatcc
    22321 cctcagatga gataatatga ttaatatctg tcgggtagct cttgatagga taatgcaggt
    22381 aagaagcaat gccatttttt ttgagttgct tgcgaaaggc atctgcagcg ggttggttat
    22441 tgtattgggt aataacgaca gaacctacgt aaatatctaa cgtgttaaat ttgtcaatta
    22501 agcgaaagac ttcttggtca taagaaatgc ctaaatcacc acgcgcttta gaatgttcaa
    22561 tgttattagc gttaatggca attacaattt cgacttgttc tttgagctct ttgagtaatt
    22621 taatcttatt gtcaggttca tagccaggaa gtacacgggc agcatggtaa tcttctaaca
    22681 ttttaccacc aaattccatg taaagtttgc catcaaattg gctgatacgt tctaaaatat
    22741 gatctctttg caagtttaaa tacttgttag aatcaaaagc aatagttttc attttttcac
    22801 ctctgtaaag cattataaca aagaaattcc aagaaagaaa aggttttttg cagggatacc
    22861 tcattttgtg actttttaaa aaaacttagg cttggttctt agaagaaaaa tgtatccgct
    22921 atgttcaaaa gaacttgaca attaaaatga gctttactat actagtaaca attaaatact
    22981 actttaaaca tgttccgtga agcatgaaag tcttaacatt aaagagagtt tttttgtcat
    23041 ctcttattat ttagtgaact ttgatggact tgcggacttg tttccaagtc tttttttgac
    23101 ctttggacaa agggtattca attaattaag gtagtcagtg agctactatt aagaaggaga
    23161 caattatgtc aacattatca ttggacacca caaataagcg agctttagtg gcagctattg
    23221 cagcttcagg aacggatgac ttgaatgtca tgtttcttgc cttttccatg tcttccatta
    23281 tgacagactt gagtcttagt ggcactcagg gtggctggat tgcgaccatt accaatttag
    23341 gaatgttggt gggaggcctc ttattcgggt tgctggctga taggcaccac aagtttaagg
    23401 tctttaaatg gacgatttta ctgttttcag tagcaacagg attaatttat tttacccagt
    23461 ctttgcccta cctgtacctg atgcgattta ttgctggcat aggagttgga ggtgagtacg
    23521 gtgtagccat tgccataatg gcagggattg taccaccaga aaaaatgggt cggatgtctt
    23581 ctttaaatgg tattgctggc cagcttggct ccattagttc ggctctttta gcagggtggc
    23641 tggctccaag tctaggctgg cgtggccttt ttctctttgg attacttccg attctcttgg
    23701 tgatttggat gactttagcc atagatgacc aaaaaatttg ggatcattat gggcaagagg
    23761 aggaagaatg cagtcaacca attaaaatca atgaactatt caaaacaaaa tccttaacag
    23821 ctcaaaccct tgctttaatg gtaatgacga ctgtgcagat tgcgggctat tttggaatga
    23881 tgaattggtt gccaaccatt attcaaacaa gcttaaacct ttcagtaaaa agttcgtcct
    23941 tgtggatggt ggctactatt gtcggcatgt gtttggggat gctgtgtttt ggtcagcttt
    24001 tagattgctt tggtccacgt ctgatttatt ccctcttttt attagcatca tctatttgtg
    24061 tttacctttt tcaatttgcc aattccatgg cgagtatggt tattggaggt gctattgtcg
    24121 gtttttttgt caatgggatg tttgctggct acggggctat gattaccaga ctttatcccc
    24181 atcacattcg atccacggct aataatgtta ttttgaatgt tggccgtgct ctaggaggat
    24241 tttcgtccgt tgctattggt agtattttag atgcctcagg tatttcaatg gtcatgattt
    24301 tcctagcttc tctgtatgtg attagttttg gagctatgtg gtctattggt cagttaaaag
    24361 cagaacgcta ccaacaattg cgttagcaaa gagatgggtg ttagtatttg agttactttc
    24421 actcacaaag ctattttatt gattgagata accaaaaagg taagcactta gggattgctt
    24481 acctttttgg ttcttttctt ttgttttact ccgctacttt ggccataaaa tgtttactgt
    24541 tcaccctaac tctagtatga caggcttttc caatgagttg ttctcccaca aaagcttcca
    24601 agcggaaatc gtattttcgg ccttcttctt taagggctgt aatgatgatt gtaacaggat
    24661 ctccaatggc tgaagctgcc aaatgttgga tagctatctc gctgccaacc gttgtaaagt
    24721 cactgtcgag ctgttcttgg gcaaataagt aagaagcatt ttccataaaa gcaacaagag
    24781 caggagtagc taggacattc agcgttcctg agcccatggc tttagccgaa tgattgggac
    24841 gggtttcata agtgtgagaa taaatagtca taagttaatc tcctcgtttc tataagccta
    24901 ttataatata ttaagcgctt acagtcatga aactactgtt tttttatgtt atacttaata
    24961 atgaattttc tttgaaaggg ttatgatgcg cttagataaa tttttagtgg caactggtgt
    25021 tggaactagg agccaagtca aacttctctt aaaaaagaag gctattttcg tcaatcaaaa
    25081 ggttgaaacc tctgccaaag cacacattga tgaatacaag gatttggtga cgtatcaagg
    25141 cacgcccttg gtctatgaat cctttgttta ttacctgtta aacaagccgt cagggtatgt
    25201 gtcagcaacc caggacagac agcaggctac tgttatggag ttgttagatg acacggctag
    25261 gcaaaaagca gtttttcctg tgggtaggtt agataaggat acccgtggac ttttgctctt
    25321 gaccaataat ggacaactag cccatgacct tttatcccct aaaaaacatg ttacgaaaga
    25381 gtacttggct aaagtagtag gcatcatgac tgaagctgat aaagattatt ttgctagagg
    25441 tatttcctta aaggatcacc agtgcctacc tgctcattta gaagtgcttg ctagtgattt
    25501 acagcagcga acaagtttgg ttaaaattac catacaagaa ggaaaattcc atcaggtgaa
    25561 acgcatggta gcagcctgtg gaaagggagt gcttgacttg cagcgtctca gcatgggacc
    25621 attaaaacta gaccctagcc ttgcagaagg ggagtttagg cgcctgacgc ctgaggaatt
    25681 gcaaagcctg gcaccttatt gtcagagctc actataaaaa gaaacctccc ctgtcagtac
    25741 tgaccccaaa aagttggaca aataattatt gaaaggattt agttctgtat tggacaggac
    25801 taaatccttt tagttttgct ttaattcgtt tgttgttgta gcaaaaaatg taatctgtaa
    25861 tagcttcttc aagcttatca agtgattgat aagttgtctc gagtccataa aacatttcag
    25921 atttgagaat gccaaagaag gaatccatca tgccgttatc cgggctattc cctttgcggg
    25981 acgtggatgg caaaatacct ttagactcta aaaaatcatg gtaggactgg tgttggtatt
    26041 gccacccctg gtcactatga agaatagttc cgctgtatga atccgctgga aaagtcttct
    26101 caagcatggt ttgaacctgt ttcaagttag gagaccgaga cagagtgaaa tcaataatct
    26161 cactgttata gccgtcaaga acaggtaata agtagagttt tccttcaggt aaggccaact
    26221 ccgtcacatc agtgtaacat ttttcgtaag gtttagagcc ttcgaagtga cgtttaatca
    26281 gattatcagc cttcttacca acctctcctt tgtaagaaga atacttgcgc ttccgacgaa
    26341 tgcgggctgc taggctcatg actttcatga gacgttgaac
//
LOCUS       DAUVUY010000014        25528 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.014, whole genome shotgun sequence.
ACCESSION   DAUVUY010000014 DAUVUY010000000
VERSION     DAUVUY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25528
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.014"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(412..900)
                     /locus_tag="VOX58_000565"
     CDS             complement(412..900)
                     /locus_tag="VOX58_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877816.1"
                     /translation="MLELSIENIIKPMKTQGKTRVTGSIGDQAIRIDLDGLVIHYNGQ
                     GLLLETIPGTYGGKRYFFVCPDCERRCRKLFKASHAFACGSCQKVHQATLNRSKTDCC
                     YYWQLAFKECLKVNPEARHIHGYYSRDDFPKRPKYMRLTKYLYHWRRFHYYMDKGDRY
                     WL"
     gene            complement(974..1483)
                     /locus_tag="VOX58_000566"
     CDS             complement(974..1483)
                     /locus_tag="VOX58_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877817.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGTYEQPVID
                     ATYHNDDQGGRANQLVLKYVEDRKLNKVFKRICDSFKDYYNQPEDMTDEELDHQRVVS
                     LSKSTQGKVNNRDTLIAFISDHNQLAEHLSMNYKEMTPEDLGAILESISQAFNHLYDM
                     VVEGQLLVK"
     gene            complement(1485..1658)
                     /locus_tag="VOX58_000567"
     CDS             complement(1485..1658)
                     /locus_tag="VOX58_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877818.1"
                     /translation="MKLKLHTRGGNTITIQGDRTLYNELVKYLLSGEQPNWVASPSAI
                     INLSDIIAITKEK"
     gene            complement(1664..1837)
                     /locus_tag="VOX58_000568"
     CDS             complement(1664..1837)
                     /locus_tag="VOX58_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HES5877819.1"
                     /translation="MKIKLFYQKHNESLDDFEHRVNLFTLSVSVIDIKFQEATYGNYE
                     DMDSRTGLLVLYR"
     gene            complement(1834..2088)
                     /locus_tag="VOX58_000569"
     CDS             complement(1834..2088)
                     /locus_tag="VOX58_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877820.1"
                     /translation="MNPNYTNHGVKKMRYNYDDLPNDQLYRLELPNGQCGYDFITKIN
                     FNIKSRPGDCDFTRPELTRRGYQWVFDCGFAIPVEKGAKT"
     gene            complement(2382..3776)
                     /locus_tag="VOX58_000570"
     CDS             complement(2382..3776)
                     /locus_tag="VOX58_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HES5877821.1"
                     /translation="MKDYQNKLSQATQPAFAPAFRTRKGRGDKEYVISSPYNVGKVFE
                     FYENIYTGIKYNEFEKTIEITKAVPWSKEKGLWTNEQTSLCIAFIDEKYRFTPRKEHI
                     EVAITALAKKNTYHPIKQRIESQKWDGKARGERYFIDLLGCADNSYNREIAKVWLTGL
                     MARIYLRKVKFEVVPILIDKRQGTGKSTVTKRLLPSYHTDSEIKFGKNDSDYQKIQAN
                     AIIELGELKGMSKAEIETVKSFISSDSDTYREPYERKATPHPRHCVFIGTANKKSFLK
                     DSGTERRFFPIECGINDVKKHPMEVEEDYFLQVLAEAKVWFNNYEPLTPSKELMNQLA
                     DIQEDYKVEDVDKEIIDQLLNEFQIVEGWDSLSQYEQRQYILKQLGEPLDNAQSYSDY
                     PSAQTDCLLQVTSPNHIAYLGFNQKPTQGGKALISQKIRDYLDNDDGWKKGENPARKR
                     LFKGGTPVPYYERV"
     gene            complement(3788..4654)
                     /locus_tag="VOX58_000571"
     CDS             complement(3788..4654)
                     /locus_tag="VOX58_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HES5877822.1"
                     /translation="MAIYEARGFSSYLYPYKGPLEPFDYVAQFRPLKPPEDIDIEEYK
                     RTQAPYCLSGKVTAEKNGSYKRNNASLVYRDLIFLDYDEIETGVNLPKIVSQTLWEYS
                     YIIYPTIKHTPEKPRYRLVMKPSDVMTEATYKQVVKEIADKIGLPFDLASLTWSQLQG
                     LPVTTGDPEDYQRYVNHGLDYPVPKNGSTPNRQVVTTYTPRPRSQRSITMRVIDTLFN
                     GFGDEGGRNVALTKFVGLLFNKWVDCDLETAYELVQIANSVTTKPLPIDEIDTTFRSI
                     LDKELRKRGIKP"
     gene            complement(4655..4927)
                     /locus_tag="VOX58_000572"
     CDS             complement(4655..4927)
                     /locus_tag="VOX58_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877823.1"
                     /translation="MNVFSQDYEAKLLEQNLTAFNHFLEAYQKPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLRRYQPPLEDTRKHYYKVSDILIFLGVNV"
     gene            complement(4930..5259)
                     /locus_tag="VOX58_000573"
     CDS             complement(4930..5259)
                     /locus_tag="VOX58_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877824.1"
                     /translation="MTLPENYRRVLNLIKVGADNPITGAEISLILKLEERSVQSIISS
                     LITRYNVPIIGIRHGFNRGYFIPANKEELLDGAKAFYNQVQKEQERLSVLLNADLTSY
                     KELLKGG"
     gene            complement(5271..5462)
                     /locus_tag="VOX58_000574"
     CDS             complement(5271..5462)
                     /locus_tag="VOX58_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877825.1"
                     /translation="MQELNLTPTQTLILFFVLGLLGLLLSRSKPLIEIDLPEDTHAPK
                     PRQNANYGAYIQAQNHYYN"
     gene            complement(5462..5794)
                     /locus_tag="VOX58_000575"
     CDS             complement(5462..5794)
                     /locus_tag="VOX58_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877826.1"
                     /translation="MRYRVETNPFSKDRYTPEQLEMFKNRQLSKDKAEVYFTRLYNQH
                     IAWVIIANVMTEYVIKFRKSATSFEEAWDALDYQRTTEIVFRAVNGLPCSEKDSGELE
                     TYLSEVSA"
     gene            complement(5896..6348)
                     /locus_tag="VOX58_000576"
     CDS             complement(5896..6348)
                     /locus_tag="VOX58_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877827.1"
                     /translation="MLKNQDNMIKKKRNPRVLFRNVAYKLSEIEGKTLTEIASFLGFG
                     NSEVCRSTLYKWKRKKWLKFDLKNGHYRNVEVLHEVTLEKMANKELKEQGLIYKANIY
                     YEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELFAELYTNMN"
     gene            complement(6489..6662)
                     /locus_tag="VOX58_000577"
     CDS             complement(6489..6662)
                     /locus_tag="VOX58_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HES5877828.1"
                     /translation="MSPKMGRPVKGTAKRDKRLEVRLTADEYNTIQETADKNGLSKAD
                     LIVKAVNSYESEK"
     gene            complement(6880..7053)
                     /locus_tag="VOX58_000578"
     CDS             complement(6880..7053)
                     /locus_tag="VOX58_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HES5877829.1"
                     /translation="MTDTKKRVGRPKGENSNTVKLTVRVSEELDSKLNKYAKENNLTK
                     PETLRKAFENLTK"
     gene            complement(7298..7930)
                     /locus_tag="VOX58_000579"
     CDS             complement(7298..7930)
                     /locus_tag="VOX58_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HES5877830.1"
                     /translation="MELVYMDGKKEPYTTNEIIAECAEIERISVRKLIENHKEDLEVF
                     GILSFEMTKINDGRGRPRKIYHLNEQQATLLITYLDNTKPVREFKKNLVSAFFEMRDE
                     LTKIRIERASERPKRLALNEVINRWENAPKMAYSTVYNLLLKGVTGYNKTQLTTKRGG
                     ETGIDCLNSIELAQYQALEDMAIALINLNFSYQEIKTMVFRQKEKLSQGA"
     gene            complement(7921..8667)
                     /locus_tag="VOX58_000580"
     CDS             complement(7921..8667)
                     /locus_tag="VOX58_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO-like protein"
                     /protein_id="HES5877831.1"
                     /translation="MKRNYTRVIDEMRTTHGLNLVAIGQRIGIDPRTVGKWAQGKHKP
                     NKESRKRLNKLYREVKQDMTTQVSIFEETTDQGQLLQAVTTTIFHGQALDIYGDFDTP
                     LFRAGEVARMIDYSNSSLSKFLEMVDEDEKDKKKVLTPGGVQQVWFLTEQGLYEILFQ
                     SRKPKAKEFKKWVKQVLKDIRKQGYYMEGELVEEPQTTIKAPDTLAEAERYYIDTLAK
                     AIAEVQNMDEKSRLTSKLTRFMQEVEPQWN"
     gene            complement(8684..9091)
                     /locus_tag="VOX58_000581"
     CDS             complement(8684..9091)
                     /locus_tag="VOX58_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877832.1"
                     /translation="MAKQPFTQETLYKLYEDNGVITLALIAEALPTWTLADIKQRLSG
                     WRYRKAIDYKLEDDELIDFEFLRNKKAQQVEITEGHRLKLDLLFRQVLATSEIIEKST
                     AKASDRNKAMELQQKAMRAIPDAVFKELSEVYD"
     gene            complement(9106..9309)
                     /locus_tag="VOX58_000582"
     CDS             complement(9106..9309)
                     /locus_tag="VOX58_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HES5877833.1"
                     /translation="MSKLKGYRVMLGLTQQAMADKLNISLQSYNNKETKKTPFNDKER
                     LAIKSMVAEIKPDITIDELFYQD"
     gene            9503..10018
                     /locus_tag="VOX58_000583"
     CDS             9503..10018
                     /locus_tag="VOX58_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5877834.1"
                     /translation="MNRLKELRKEKSLTQQGLADEIGITKRTYIYWEQGERQIKPDKA
                     QQLADYFKVSVGYLLGYTNNPKLYSDEIAIEPEEGVVLTHSEQRNNEKLDSIRKKTQK
                     QKDDFVNFLSENQFFLDNQTIDSILILIKNLDLDSSDPWRVKLSEMIVNDTLPADLRQ
                     KYSYIIDKGMY"
     gene            complement(10270..10797)
                     /locus_tag="VOX58_000584"
     CDS             complement(10270..10797)
                     /locus_tag="VOX58_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HES5877835.1"
                     /translation="MTMRYLLEAFGVLSFEIHKPEKGSLGGRPRRIYHLNEQQATLLV
                     TYLGNTEPVREFKKNLVKAFFEMREELTQIRLQRSLEAPKRKTLNEAIKTWEHAPKMA
                     YPTVYNLLLKAVTGKNSKQLKVTRGGYSDIDCLNSIELAQYTALEYMAIALINLNFTY
                     QDIKTMALKNTLQCA"
     gene            complement(11209..11616)
                     /locus_tag="VOX58_000585"
     CDS             complement(11209..11616)
                     /locus_tag="VOX58_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877836.1"
                     /translation="MARTPFTQELLHQIFDDTGTMSLELIAERLPDWSEKDIKLRLAA
                     WRYRNNIDYTMANGEIDTFEIINNRKAISEEVSAGRQLKLEEYFKQVQATAEIINKPT
                     ASDTNRLKAIQLQQVAMDEIPDQYFKELTELYG"
     gene            complement(11637..11837)
                     /locus_tag="VOX58_000586"
     CDS             complement(11637..11837)
                     /locus_tag="VOX58_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5877837.1"
                     /translation="MTVGDKIKTIRLNLGETMEEFGKRFGTSKGTVNNWEKGRNLPNR
                     NSLLRIAQLANQTVSDFLAITD"
     gene            complement(11871..12077)
                     /locus_tag="VOX58_000587"
     CDS             complement(11871..12077)
                     /locus_tag="VOX58_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5877838.1"
                     /translation="MTQITLKAARINAGYTLKQVAGAVGKNPQTISKYEKDSSDISLG
                     LLQKLSSLYGVTIDNLFLGKKVRK"
     gene            12231..12998
                     /locus_tag="VOX58_000588"
     CDS             12231..12998
                     /locus_tag="VOX58_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5877839.1"
                     /translation="MAKNSPQDLINREIFSTNLNMLMAKKNIKQIDIHNKLGIPKSTI
                     TGYVKGRSLPTAGNVQKLADFFGVLKSDIDPRFDSNNIETNSNIIPSTLQKVTSTLSQ
                     LEHKRQLNVLDYAETQLEQQNTVEEPQATYYTYNYYDHAASAGTGQYLNDVQVETIEL
                     PVDYDADFVIPVYGDSMEPEYHSGDYVFIKLSINLSDGDIGVFEYYGDAYIKQLVIND
                     SGAFLHSLNDKYDDILIDRDSDFRIIGEVIGSFTSKS"
     gene            complement(13860..14003)
                     /locus_tag="VOX58_000589"
     CDS             complement(13860..14003)
                     /locus_tag="VOX58_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HES5877840.1"
                     /translation="MSGGGAYVCKSQKPKERRRQGLSVYETLTLMIAFGTLIVAIMNN
                     KNK"
     gene            14252..15403
                     /locus_tag="VOX58_000590"
     CDS             14252..15403
                     /locus_tag="VOX58_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HES5877841.1"
                     /translation="MIMKITEHKKKNGTIVYRASIYLGIDQMTGKRVKTSITGRTRKE
                     VNQKAKHAQFDFLSNGSTIKRKVVIKTFKELSHLWLETYKLTVKPQTYDATVTRLNRH
                     IMPTLGNMKVDKITASDIQMLINRLSKYYVNYTAVRSVIRKVLQQGVLLGLIDYNSAR
                     DIILPRKQPNAKKKVKFIDPSDLKSFLEHLETSQHKRYNLYFDAVLYQLLLSTGLRIG
                     EACALEWGDIDLENGTIAINKTYNKNLKFLSTAKTQSGNRVISVDKKTLRSLKLYQMR
                     QRQLFNEVGARVSEVVFATPTRKYFNASVRQSALDTRCKEAGIERFTFHAFRHTHASL
                     LLNAGISYKELQYRLGHANISMTLDTYGHLSKDKEKEAVLYYEKAMNNL"
     gene            15498..17480
                     /gene="mutL"
                     /locus_tag="VOX58_000591"
     CDS             15498..17480
                     /gene="mutL"
                     /locus_tag="VOX58_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HES5877842.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLAISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQFRGLYYDPQKNDFFVKESAVSEKIPETDFYFGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNTFIETDHPNLDLKNRQKLSQMLTRLE
                     NEEQSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGEVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            17491..18711
                     /locus_tag="VOX58_000592"
     CDS             17491..18711
                     /locus_tag="VOX58_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HES5877843.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFVGILLVEIASNFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLASFQNYRQVFHDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            18713..19309
                     /gene="ruvA"
                     /locus_tag="VOX58_000593"
     CDS             18713..19309
                     /gene="ruvA"
                     /locus_tag="VOX58_000593"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HES5877844.1"
                     /translation="MYDYIKGQLTKITAKYIVVETNGLGYIINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGNTKARSNKAGNTQLDEAIEAL
                     LALGYKAAELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            19319..19879
                     /locus_tag="VOX58_000594"
     CDS             19319..19879
                     /locus_tag="VOX58_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HES5877845.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLHDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVAAFTSEEMADALENPSIIRHKLKLAATVNNAIA
                     VQKIQEEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            19948..21219
                     /locus_tag="VOX58_000595"
     CDS             19948..21219
                     /locus_tag="VOX58_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HES5877846.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            21308..22444
                     /gene="recA"
                     /locus_tag="VOX58_000596"
     CDS             21308..22444
                     /gene="recA"
                     /locus_tag="VOX58_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HES5877847.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPELFD
                     EIDRKVRVKFGLLEESEEESAMVVASEEIDDLALDLDNGIEIED"
     gene            22735..23133
                     /gene="spx"
                     /locus_tag="VOX58_000597"
     CDS             22735..23133
                     /gene="spx"
                     /locus_tag="VOX58_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HES5877848.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
     gene            23247..23516
                     /locus_tag="VOX58_000598"
     CDS             23247..23516
                     /locus_tag="VOX58_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HES5877849.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            23513..23932
                     /gene="ruvX"
                     /locus_tag="VOX58_000599"
     CDS             23513..23932
                     /gene="ruvX"
                     /locus_tag="VOX58_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HES5877850.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            23944..24249
                     /locus_tag="VOX58_000600"
     CDS             23944..24249
                     /locus_tag="VOX58_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HES5877851.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            24663..>25528
                     /locus_tag="VOX58_000601"
     CDS             24663..>25528
                     /locus_tag="VOX58_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HES5877852.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDAFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTCFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFA"
BASE COUNT         7466 a         4601 c         4937 g         8524 t
ORIGIN      
        1 gcgctcatgg tcttttctcc tggttatggt ataatttatt taagcttaaa tttaaccaag
       61 ggggcgttcg tatggacgtc tttttgtttt gttcgttttg taaactagat attttttgta
      121 gtacttcgct tttcgcttct aaaatcgttt ctaagcactt tttagagttt atagtataaa
      181 tcatcaactt agtagctaga agcgctaaaa caatgtttta ttttagtctt agatggttat
      241 tctttgtggc agggttatgg gctaaaatga gatgtcaaat cctttcctat caagcttttt
      301 aatcttcgtc aaaaatttca aaaagggaat tttttgcacg gaaaagggcg cgttcttaag
      361 tttccgaaca atatagtccc gtttaaaaat gaagggggta gtttccgaat attatagcca
      421 gtacctgtca cccttatcca tatagtaatg aaatcttctc cagtgataaa ggtatttggt
      481 taatctcatg tactttggac gtttaggaaa gtcatcacga ctataatatc catgaatgtg
      541 tcttgcttct ggatttactt tcaaacactc tttaaaggct aattgccagt agtagcaaca
      601 gtctgtcttg cttcgattga gcgtggcttg atgaaccttc tgacaagaac cacaagcaaa
      661 ggcatgagaa gccttaaata atttccgaca acgtctctca cagtcaggac acacgaaaaa
      721 gtaacgttta ccaccataag ttcctggaat cgtttcaagc aataggcctt gaccattgta
      781 gtgaatcact agcccatcta ggtctatgcg gatagcttgg tcacctattg atcctgtaac
      841 tcttgtcttc ccctgtgtct tcattggttt aatgatattc tcaatagata gttctaacat
      901 tatttctcct taaaactcaa acaacccaaa actattgata aaacgacaaa aagaggaaaa
      961 accctctgaa tgtctattta acgagtaact gaccttcaac aaccatatca tacaaatggt
     1021 taaaggcttg actgatagac tcaaggatgg cccctaagtc ttctggcgtc atctctttat
     1081 aattcataga gaggtgttca gccagttggt tgtgatcgga gatgaaggct ataagtgtgt
     1141 ctcgattatt aactttccct tgagtacttt tagataaaga aaccacgcgc tgatgatcaa
     1201 gttcttcatc cgtcatatcc tcaggttggt tgtaatagtc cttgaagctg tcacagatac
     1261 gcttgaagac tttgtttagt tttctgtctt caacatattt taagactaac tgattagcac
     1321 gaccaccttg gtcatcattg tgataagttg cgtcaatcac tggttgctca taagtaccag
     1381 tcatatagcc taaaatagca tgacaagcca cttgtgcggt gtcaaagtct ttaaacgtgt
     1441 agtggaatgt gaatgtcttt ggtgtgtctg aaaatgttct catgctattt ctcctttgtg
     1501 attgctataa tgtctgataa attgataatg gcagaaggag atgctaccca gtttggttgt
     1561 tctccagata aaagatactt gaccaactca ttataaaggg tgcggtctcc ttgtatggtg
     1621 atggtgttac cacctcgtgt gtgtaatttt agtttcatat cagttacctg tacaagacca
     1681 gtagtcctgt tcttgaatcc atgtcttcat aattgccata agtggcttct tggaatttaa
     1741 tgtctatcac agataccgat agggtaaaga gattgacccg atgttcaaaa tcatctagtg
     1801 attcattgtg tttttgataa aatagtttga ttttcatgtt ttagctcctt tctcaacagg
     1861 tatcgcaaag ccacagtcaa agacccattg gtaaccacgt cgtgttagtt ctggtctggt
     1921 aaaatcacaa tcacccggtc tagactttat gttgaaatta attttggtga taaagtcata
     1981 accgcattga ccattaggaa gttccaatcg gtatagctgg tcgtttggca aatcatcata
     2041 attgtaacgc attttcttta cgccatgatt tgtatagtta ggattcatat gtccctcttt
     2101 tctctctaaa atgtgtgctt tttgtttttt taaaaatcaa atagcacagc aaaaagcaca
     2161 gggataaagc cagtggtatc aagggggttg ggcactttgt gcttttagta gcattaattt
     2221 gagcaaaaga ctttcttttt ttgcacgcgc actattagtt ataaatatta ttatctataa
     2281 tattaaatac tactaatact acaatagagt ataaagccta taataccaag gttttagctt
     2341 gtatcattac gtgtagtttt tatgtagcat tagtgtagtt tttaaactcg ttcatagtaa
     2401 ggtacgggag ttccaccttt aaatagtctt tttcttgcgg gattctctcc ttttttccag
     2461 ccgtcatcat tatctaagta atcacgtatt ttttgagaaa taagggcctt accaccttgc
     2521 gttggttttt ggttaaatcc tagataagcg atatggttag gacttgtcac ttggagtaag
     2581 caatctgtct gtgcggaagg gtagtcacta tagctttgag cattatctaa cggctctcct
     2641 agctgtttga ggatgtattg ccgttgttca tactgtgata agctatccca accttcaaca
     2701 atttgaaact cattcagtaa ttgatcgata atttctttgt cgacgtcttc aaccttataa
     2761 tcttcttgaa tgtctgctaa ctgattcatt aactctttag atggcgttag tggttcataa
     2821 ttgttaaacc atactttggc ttcagcgagt acctgtaaga aataatcttc ttccacctcc
     2881 ataggatgtt ttttcacgtc attgatacca cattcaatag ggaaaaagcg tctttctgtt
     2941 ccactatcct taagaaaaga ttttttatta gctgttccga taaagacaca gtgccttgga
     3001 tgaggagtgg ctttacgttc ataaggttca cgataagtat cactatctga ggaaatgaag
     3061 cttttaactg tttcaatttc tgcctttgac atgcctttta gctcccctag ctcaataatg
     3121 gcattggctt gtatcttctg ataatcacta tcatttttac caaacttgat ttctgaatca
     3181 gtgtggtagc tagggagtag tcgcttagta acggtgcttt tcccagttcc ttgtctttta
     3241 tcaatgagaa taggaacgac ttcaaacttt actttacgga gataaattct agccatgaga
     3301 cctgttagcc atactttggc aatttctcta ttataggaat tatcagcaca gcctaataga
     3361 tcaataaagt agcgttctcc tctagcttta ccatcccatt tttgactttc aatacgctgt
     3421 ttaatggggt gataagtgtt ctttttagct aaagcggtaa tagctacctc tatatgttct
     3481 ttacgagggg taaaccgata tttttcatca atgaatgcaa tacaaaggct ggtctgctcg
     3541 ttcgtccata gccctttttc tttagaccaa ggaactgctt tagtgatttc aatagttttt
     3601 tcaaattcgt tgtatttaat acctgtatag atattttcgt aaaattcaaa aaccttaccg
     3661 acattgtagg ggctactaat gacatattct ttgtctcctc taccttttcg tgtcctgaaa
     3721 gcaggggcaa aagcaggttg agtggcttgc gatagtttat tttgatagtc tttcaattct
     3781 tctttgtcta tggcttgatt cctctctttc ttaattcttt atcaagtatg cttctaaagg
     3841 ttgtatctat ctcatcaatg ggtagtggct tagttgtcac gctgttagct atttgtacca
     3901 gctcataagc cgtctctaaa tcacaatcca cccatttatt aaatagcaag ccaacaaact
     3961 tagttaaggc cacgttgcgc ccgccttcgt ctccaaaacc attaaacaag gtatctatga
     4021 ccctcatggt aatagaacgc tgacttctag ggcgtggcgt gtaagtagta acaacttgtc
     4081 tgtttggcgt gctaccattt ttaggaacag gataatcaag accatggttc acatagcgct
     4141 gatagtcctc tgggtcgcct gttgtaacgg gtaagccttg taattgcgac caggtaagac
     4201 tagctaaatc aaacggcagt ccaatcttat cggctatctc cttgaccact tgtttataag
     4261 ttgcttcagt catcacgtca ctaggcttca tgacaaggcg ataacggggc ttctcggggg
     4321 tgtgtttaat cgttggataa ataatataac tatactccca aagcgtctga gaaacgattt
     4381 taggtaggtt gacgcctgtt tctatctcgt catagtcaag aaaaatcaaa tcgcgataaa
     4441 ctaaactagc attattgcgc ttatagctac cgtttttctc tgctgtgacc ttgccactta
     4501 ggcagtaggg ggcttgtgtt cgcttgtatt cttcaatatc aatatcctca ggcggtttca
     4561 aaggtctaaa ctgagcaaca tagtcaaatg gttctaaagg tcctttgtag gggtacaaat
     4621 aagagctaaa gcctcttgct tcataaatag ccatctacac atttaccccc aaaaagataa
     4681 gaatatcact gaccttgtaa taatgtttcc tggtgtcttc tagtggtggt tggtatcgtc
     4741 ttaacccagc attttcccac cgttttaggg ttttaccttt gatatttaat tcctctttga
     4801 cttgttcggc cgtgatcaac cctaaaactc ttggtttagg tttctggtag gcttccaaaa
     4861 agtgattaaa agcggtcagg ttttgttcta agagtttggc ttcataatct tgactaaata
     4921 cgttcatgcc taacctcctt tgagtaattc cttataactg gttaaatcgg cattcaataa
     4981 cacacttagg cgttcctgtt ccttttgtac ttgattataa aaggctttag caccatctag
     5041 taattcttct ttgttagctg ggataaagta cccacgattg aatccgtgtc taatgccgat
     5101 aatagggacg ttatagcgcg tgattaagct actgatgata ctttggacgg agcgttcttc
     5161 aagtttcagt attaagctaa tctctgcccc tgtaatgggg ttgtctgctc caaccttgat
     5221 cagattaagg acacgtctat aattctctgg tagtgtcatt cagttcctcc ctaattgtaa
     5281 taatggttct gtgcttgaat ataagcccca tagtttgcgt tctgacgtgg tttaggtgcg
     5341 tgggtatctt ctggtaagtc aatctctatt aacggcttag aacggctaag aagaagccct
     5401 aagagaccta aaacaaagaa tagaataagc gtctgtgttg gtgtgaggtt aagttcttgc
     5461 atcatgccga tacctcactt aaataagttt ctagttcccc tgagtctttc tctgaacaag
     5521 gtaaaccgtt aacggctcta aagacaatct ctgtggttcg ttgatagtct aaagcgtccc
     5581 atgcttcttc aaagctggtg gcactttttc tgaatttaat gacgtactct gtcataacgt
     5641 tagcaataat tacccaagca atatgttggt tatatagtcg agtgaaatag acttcagctt
     5701 tatctttgct gagttggcga tttttgaaca tttctagctg ttcaggagtg tatctatctt
     5761 ttgaaaaagg atttgtttct actctatatc tcattatgtt ttttctcgct taattattat
     5821 tttctgtgta gtgtttttat tgattgcttg tttcttatac tagattcatg ctagttttaa
     5881 ggggtagctc cctgattagt tcatgttagt gtataattct gcgaataact cgctagggat
     5941 acgctctagc gctttttgtt gtaagtggat ggctttaatt ctatcttgtg ttttggtttt
     6001 aatgtcttct ataatttcag atgttgagac cacttgttca tagtaaatgt tagctttata
     6061 aatgagtcct tgttctttta attccttatt agccattttt tcaagcgtaa cttcatgtaa
     6121 tacttcaaca ttacgataat gaccgttttt gaggtcgaat tttagccatt ttttacgctt
     6181 ccatttatat agggtgcttc tgcaaacttc tgaattccca aaaccaagaa aagaagcgat
     6241 ttctgttaat gtttttcctt caatttcaga tagtttataa gcgacgtttc taaataacac
     6301 tctcggattt ctttttttct taatcatatt gtcttgattt ttgagcacac aaaaagcgca
     6361 ctccctttct atgaatttta ggttcacaaa atagagtacg catgatatac tatttacgta
     6421 cctactttgt gggtgctggg agttcctaac gtgtacggtc gccaaactat accacgttag
     6481 gaactttttt atttttcaga ctcataagag tttacagctt taacgattaa atccgcttta
     6541 gataatccat ttttatccgc tgtttcttgt attgtattat actcgtcagc ggtcaaacga
     6601 acttccagcc gtttatcacg tttagctgta ccttttacag gtctgcccat ttttggactc
     6661 ataggagttc ctcctttcat ttaagcccgt gcttatatca tatgataagc acgtacataa
     6721 gtcaacccct aaattaaaaa aacatgcgta ttctattttc aatgtgcaaa tggttcacaa
     6781 aatagagtac gcatgatata ctatttacgt acctactttg tgggtgctgg ctctatcacg
     6841 ttgacggttt ggcgattgta aacgtggtag agtacttttt tattttgtaa gattttcaaa
     6901 ggcttttctc aaagtctcgg gtttagtcaa gttattctct ttagcgtact tatttagttt
     6961 gctatctaac tcttcactaa cccttaccgt taatttaaca gtatttgagt tttctccctt
     7021 tggtcgtcct actcgttttt tggtgtcggt cattgagttt atcactcctt tcatgtcgac
     7081 aaaaactatt atagtttatg tcgtcaataa ttgtcaaccc ctaaaccaaa ctttttttgc
     7141 gtactctatt ttattttcaa tgatcagtat gatacaatgg aagtatcaaa tatttactaa
     7201 aaccccttta ataatagctt gcctgcttta ttaatcgtgt tttatttccg acttaaggct
     7261 tttctgattg gtttcggtaa gccttttttc tttgctttca cgctccttgt gagagctttt
     7321 ctttttgtct aaagaccatt gtttttatct cttgatagct aaaattcaga ttgattagag
     7381 ctattgccat gtcctctaat gcttgatact gcgctaactc aatactattc aagcaatcaa
     7441 taccagtttc cccacctcgc ttagtggtta attgtgtttt gttatagccc gtaacacctt
     7501 ttagcaaaag attataaaca gtagaataag ccattttagg ggcgttctcc caacggttaa
     7561 taacttcgtt aagtgccaag cgtttaggcc tttcggaagc tcgctctatc ctaatcttgg
     7621 ttaactcgtc cctcatctca aagaaagcac taactaaatt ctttttaaac tctcttacgg
     7681 gtttggtgtt atccaagtaa gtgataagta gtgtcgcttg ttgttcgtta agatgataaa
     7741 tttttctagg gcgaccacga ccgtcattta tttttgtcat ttcaaatgac aatattccaa
     7801 acacctctaa atcttcttta tggttttcaa tcaattttct aactgaaata cgttcaattt
     7861 cagcacattc tgcgataatt tcattcgttg tgtacggctc tttcttgccg tccatgtaga
     7921 ctaattccat tgtggttcta cctcctgcat gaatcgggtt agcttgcttg tcaggcggct
     7981 cttctcgtcc atattctgaa cctctgcaat ggctttggct agtgtatcga tataataccg
     8041 ctctgcctct gccaacgtgt caggggcttt tatagtggtc tgtggttctt ccaccagttc
     8101 cccttccatg tagtagcctt gttttctaat atcttttaat acttgcttaa cccatttctt
     8161 aaactcttta gcttttggtt tgcgtgattg gaatagaatc tcatataatc cttgttctgt
     8221 tagaaaccag acttgttgaa cgcctccagg ggtaagaact ttcttcttgt ccttttcgtc
     8281 ttcgtctacc atttcaagaa acttactcaa actactattt gagtagtcaa tcattctagc
     8341 aacctcccca gctctaaaaa gtggggtgtc aaaatctccg tagatatcca acgcttgacc
     8401 gtggaaaata gtagtagtga cagcctgcaa tagttgacct tggtctgttg tctcttcaaa
     8461 aatacttact tgtgttgtca tgtcttgctt aacctctctg tataacttgt ttagtcgttt
     8521 tctgctttcc ttgttgggct tatgtttgcc ctgtgcccac ttgccaactg ttcgggggtc
     8581 tatgcctatt cgttggccaa tagctaccaa gttcagccca tgagtggttc gcatttcatc
     8641 gatgaccctt gtatagttcc tcttcattgc ttgcctgcct ttcttaatcg taaacttctg
     8701 atagctcttt aaaaacagcg tcagggattg cccgcattgc tttttgttgc agttccattg
     8761 ctttgttacg gtcgctcgct ttggcggtgc ttttttcgat aatttcagaa gttgcgagaa
     8821 cttgccgaaa aagtaagtct aatttgagtc tatgcccttc tgtaatttct acttgttggg
     8881 cttttttatt tctcaaaaat tcaaaatcta tcagctcatc atcttctaac ttatagtcaa
     8941 tagcctttcg ataccgccaa cccgaaagcc gctgcttaat gtctgcaagc gtccacgttg
     9001 gcaaagcttc agcgataaga gccaaagtga taactccgtt atcttcatag agcttataga
     9061 gtgtttcttg ggtaaatggt tgttttgcca ttggtttgct cctttctagt cttgataaaa
     9121 tagttcatca atggtgatgt ctggtttaat ttcagcaacc attgacttaa tcgctaggcg
     9181 ctctttgtca ttgaagggcg ttttcttcgt ctctttgtta ttgtaagatt gcaaagagat
     9241 gttcagcttg tctgccattg cttgttgtgt tagcccaagc atgacacgat agccttttaa
     9301 tttgctcatg gtgtttctcc tttctagatg aatagattga aaaagtgaaa ggttttggtg
     9361 aattttttgt gcaacttatg ttgctgcttt tgattatagt gtacatttta ttcaatgtca
     9421 atagtttttt gaaaaaaatt gtatttttcg ttcacttttt tcaaaaaatg atttataatc
     9481 aatcgtgaaa ggtcgttaaa ttatgaatag attgaaagaa ctcagaaaag aaaaaagctt
     9541 gacccaacaa ggtctagctg atgaaatagg tatcacaaaa agaacttata tctattggga
     9601 acaaggagag cgccaaataa aaccagacaa agcccagcaa ttagctgact acttcaaggt
     9661 aagcgttggg tatctgttgg ggtatactaa taatcccaag ctatacagtg atgaaatagc
     9721 aattgaacca gaggaagggg tggtgttaac tcattcagaa caacgaaaca atgaaaagct
     9781 agactcgata agaaaaaaaa cacaaaagca aaaagatgat tttgttaact tcctgtctga
     9841 aaatcagttc tttttagata atcagaccat agatagcatt ttgatactga taaagaattt
     9901 agatttagat agctctgacc cttggcgggt aaaactctcg gaaatgattg taaatgatac
     9961 tttacccgct gacttaaggc aaaaatatag ttatataatt gataaaggaa tgtattgacc
    10021 cacgcgccaa catttactaa cattttggtt tacagtagcg ctcaattttg agctgtttct
    10081 acactatgat tttctagtat tttctagtat ttctaacctg ctttcctaat ttaggactac
    10141 agttatccca acaattcata taaacctaaa acctttttaa taatggcttg cctgctttat
    10201 taattgagtt tagttatact agttgaaggc ttggaagttt ggtcgctgtc aaagcctttt
    10261 ttgttgtttt cacgcgcatt gtagcgtgtt ttttaatgcc attgtcttaa tatcttgata
    10321 ggtaaaatta agattgataa gagcgattgc catatattct aaagcagtgt actgtgctag
    10381 ttcaattgag tttaagcagt caatatcaga ataaccacct ctggttactt ttaattgctt
    10441 gctattctta ccagtaacag ctttaagcaa tagattgtac acagtcggat aagccatttt
    10501 aggcgcgtgt tcccatgtct tgatagcttc attaagtgtt tttcgtttag gagcttcaag
    10561 agaacgctgt aacctaattt gagtgagttc ttctcgcatt tcaaagaatg ccttgactag
    10621 gttcttttta aactctctga ctggttcggt atttcctaga taggtaacta ataaagtcgc
    10681 ctgctgttcg tttaagtgat aaattcttct aggtcgtccg cctagtgaac ctttttcagg
    10741 tttatggatt tcaaatgaca aaaccccaaa cgcttctaaa aggtaacgca tggtcacgca
    10801 aagttacgca gaattttcca tgttgcgtaa cctactcaat ctctttagta ccaatcgatt
    10861 tcagcattta tttaagaaag gttacgcagt aacaaacaat aagtctaatt aatgatttat
    10921 taattattta tttatatata ggggtagggt agggtgattt tgcgttactg cgtaaccatc
    10981 cttctctaac gctatctata tcaacgtttt agaggttacg tagtaaaaaa gggggtgcgt
    11041 taccttatgt accacacaac agcactttcg ttcttaatcc tttttatgct tatcaatcag
    11101 ctttctaact gaaataatgt caatttcagc atgttcagct atgatgtcgt gtgtggtgta
    11161 aggctctttc ttaccatcca tgtaaactag gttcatggta atcctccttt atccatagag
    11221 ttccgttagt tctttaaaat actgatcagg aatttcatcc atagccactt gttgtaattg
    11281 aatggccttt aaacgattgg tgtcgctagc agtcggttta ttaataattt cagccgttgc
    11341 ctgtacttgc ttgaaatact cttcaagctt aagttgcctt cctgcggaaa cttcttcgga
    11401 tatggctttt ctgttattga taatttcaaa ggtatcaatt tcaccgtttg ccatggtgta
    11461 atcaatgtta tttcgatacc gccaagctgc cagcctaagc ttgatgtctt tttcagacca
    11521 atcgggaagc cgctcagcaa tcagctctag gctcattgtt cctgtatcgt caaatatctg
    11581 atgtaataat tcttgtgtaa atggtgttct agccatttgt tatttacctg cctttcttaa
    11641 tcggtgatag ctaagaagtc gctaacagtt tgattagcga gctgtgctat tcttaataga
    11701 ctgtttctat tcgggaggtt tcttcctttc tcccaattat taacagtacc tttagaagtg
    11761 ccaaagcgtt ttccgaattc ctccatagtt tctcctaagt taaggcgaat agttttgatt
    11821 ttgtcaccca ctgtcatttc ttgcctgcct ttctaattaa taaagtacta ttattttcgt
    11881 acttttttac ctaaaaaaag gttatcaata gtgacaccgt acaaagatga taatttttgc
    11941 aatagtccta aagaaatatc ggagctatct ttttcgtact ttgaaatagt ttggggattt
    12001 ttcccaaccg ctccagctac ttgttttaaa gtgtaaccag cgttgattcg ggcagctttt
    12061 agtgtaattt gcgtcatgtt ttcaactcct ttctaaaaag ctatggctta atagtactat
    12121 tattttcgta ccaagtcaat gatttttgta gaaaaatatt aaaaaaatag tacttttagg
    12181 tttatttgtg ttagaattaa ttcagataca aaaagaaagg ggtaagagaa atggctaaaa
    12241 atagtcccca agatttaata aatagagaaa ttttctcaac aaatctcaac atgcttatgg
    12301 ctaaaaagaa tatcaaacag atagatattc acaacaaact aggaatacct aagagtacga
    12361 taactggcta tgttaaaggt cgttcactcc caactgctgg aaacgttcaa aagctggcgg
    12421 acttcttcgg agttctaaaa tcagacattg acccccgttt tgattctaat aatattgaaa
    12481 caaatagtaa tattatccca tcaaccctac aaaaagtaac atctacttta tctcagctag
    12541 aacacaagcg acaattaaac gtccttgatt atgctgaaac acaattagaa caacaaaaca
    12601 cagtagaaga accacaagcc acctactaca cttacaacta ctacgaccac gcagcttcag
    12661 ctggtacagg tcagtatcta aatgatgtac aagtagaaac aattgaatta ccagtcgatt
    12721 acgacgctga ttttgtcata ccggtttatg gcgattctat ggaacccgaa taccattctg
    12781 gggactatgt atttatcaaa ctatctatta acctgtcaga tggtgatata ggagtttttg
    12841 agtattacgg tgatgcttat atcaaacaac ttgttataaa cgattctgga gcgtttctgc
    12901 atagtctgaa cgacaagtat gacgatatac tcatagatag agatagtgat ttccgtatta
    12961 tcggagaagt tattgggagt tttacatcta aatcatgact atctgatacc cacgcgccga
    13021 attccactat tttccactaa ttttggttgc tttaccttta aggactcgca ttttgctctt
    13081 taagacagcc gaaaagtccg attttctgaa tactatacgc taaaaatgcc aactgatttt
    13141 agaagctgtc acaacggaaa aagtaaatta ataaacgacc gatatatcaa gttctttaag
    13201 tgaatttacc gagcgtttta caactattga aataggttga cgtattatgt cagtacgtgc
    13261 caacattttc cagcattccg aaatgcgacc atgtgttcgt gtttgggagt agtaaccttc
    13321 tattctctaa gttcgctcga agttcagcat tatgcgcgtg gaataaaatt agctacctta
    13381 ctgtaacctt accgttaccg ctcattttat gacctgttca attttcatgt ctaaatgcca
    13441 aatttatcat ctttacctac gcgcaaatac cttgatacgc ctttaatttt ctttaattac
    13501 aagaccctca aaacttggca aaaattgagg tataatctga acttttctga actttttgtc
    13561 ggcggtaatt aaaaaaacta tctgcgcgtg atcagtggtt gcataaagta ttataaattc
    13621 ttgcataaaa ttatttttat tcattttggt aatactaagg aatgttaaga gcgctaaaga
    13681 ctgtactttc taactacacc cttttgactt ctttatcaaa caaagctata atggacatag
    13741 aaaaaggaga ttgcgcaaac aatctcctgt ggtagcaccg tttaagacgg cagccttacc
    13801 gtatttgttt atattttcta taaaccgtcc acgattggct aaagtgtggg acggtttttc
    13861 tatttgttct tgttattcat gatagctact atcagagtac caaaggcaat catcaaagta
    13921 agcgtttcat aaactgacaa accttgtcgt ctcctttctt ttggtttctg tgacttacat
    13981 acataagcac cacctccaga cataaggcta cgactacctt gacctagttt ttagacatgc
    14041 aaaacttctt ctgaaactct ctgtacctta caaaaccaaa caaaaaagac cacgcaagtt
    14101 ttccacgctc gcaaggtctt aaaaagacta atattatacc atgattttct tttataatat
    14161 ttcggatatt tacccgatac cattattata ccatgatatg aactaatcta aaaccctttt
    14221 aataatagct tgcctgctga tggaaaggtt tatgatcatg aaaataacag aacataagaa
    14281 gaaaaacggt acaattgttt atcgtgctag tatttatcta ggcattgacc aaatgacagg
    14341 taagagagta aaaacaagca tcacaggaag aacaagaaaa gaagttaatc aaaaagccaa
    14401 gcacgcgcag tttgacttcc tatctaatgg atctacaatt aaaagaaaag ttgtgattaa
    14461 aacatttaaa gaacttagtc atttatggct tgaaacctat aagttaacag taaagcctca
    14521 aacttatgat gctactgtta ctagacttaa tcgacatatt atgccaactc tgggcaatat
    14581 gaaggttgat aagataaccg ctagtgatat tcaaatgctg attaatagat tatctaaata
    14641 ttacgtcaat tatactgcgg tacgttcagt catccgaaaa gttctccaac aaggagtatt
    14701 gctagggcta atagattata actcagcaag agatattatc cttccaagga agcagccaaa
    14761 cgctaagaaa aaagttaagt ttattgatcc gtctgatttg aaatcttttt tagaacattt
    14821 agaaactagt caacacaaac gctataacct ttactttgat gcagttctct accaactttt
    14881 attatccact ggcttgagga taggcgaagc ctgtgcatta gagtggggag atattgacct
    14941 agaaaatggt acaatagcca ttaataagac ttacaataaa aatttgaagt ttttgagtac
    15001 agctaaaacc cagtcaggca atagagtgat tagtgttgat aaaaagaccc ttagaagcct
    15061 aaagctctat caaatgagac agcgacaatt atttaatgag gttggtgcgc gtgtgtcgga
    15121 ggtagtgttt gccacaccaa cacgaaagta ttttaatgct tcggttagac aaagcgcttt
    15181 agatactagg tgtaaggaag cagggattga acgctttacc tttcacgctt ttagacacac
    15241 tcacgctagt ttattgctga acgcaggtat tagttataag gaacttcagt accgtctagg
    15301 acatgcgaat atcagcatga ctttggatac ctatggccat ctttctaagg acaaagaaaa
    15361 agaagctgtt ttatattatg aaaaggctat gaataattta taagtccaca aaaaagtcca
    15421 caaattaata ttttgagagg tgtaaaccta atgaaacctt gttatatcaa cgtttagaaa
    15481 gtgttaaaaa caaaattatg acaaacatta ttgaattacc ggaagttctc gccaaccaaa
    15541 ttgcagctgg tgaagttgta gaaaggccag cgagtgttgt caaggaattg gttgagaatg
    15601 ctattgatgc taaaagtagc cagattaccg ttgaaattga agagtctggc cttaagatga
    15661 tacaggttac agacaacggt gagggaatgt ctcatgaaga tttaccttta agtctgcgtc
    15721 gccacgctac tagtaaaatt aagagtcaga gtgatttgtt tagaattaga acacttggct
    15781 tccgtggaga ggctttaccg tctgttgcct ctatcagtaa aatcacgata aaaacagcaa
    15841 caaaagaagt cactcacggt tctcttctta tagctactgg tggggaaatt gagacacttg
    15901 aagcgatctc aactcctact ggaaccaaaa ttaaggttga aaaccttttt tacaacacgc
    15961 ctgctcgtct caaatatatg aaaagtttac aggcagaatt agctcacatt gtagatgtgg
    16021 tcaaccggtt gagtttggca catccagaag ttgctttcac actgattagt gatggtcgcc
    16081 aattaactca gacatcagga actggcgatt tacgccaagc aattgcaggg atctatggtt
    16141 tgaatactac caaaaaaatg ctggctatct ctaatgctga tttggatttt gaagtttccg
    16201 gctatgttag cttgccagag ttgacacgcg ccaaccgtaa ttacatgacg attttggtta
    16261 atgggcgtta catcaagaat ttcttgctta atcgagcaat tcttgatggt tacggttcta
    16321 agctcatggt tggacgtttc ccaattgttg tgattgatat tcagattgat ccctatttgg
    16381 ccgatgtcaa tgttcatccc acaaaacaag aggttcgtat ttcaaaagag cgtgagttga
    16441 tggctttaat tagtacggcg atttctgaaa gtcttaagga acaagatttg attccagatg
    16501 ctctggaaaa cttagccaaa tcaagcacac gacatttctc taaaccagag caaacacaac
    16561 ttcctttaca gtttagggga ctttattatg atcctcaaaa gaatgacttt tttgtcaaag
    16621 agtcggctgt ctcggaaaaa atacctgaaa ctgattttta tttcggcgct gttgacaaca
    16681 gtgtaaaggt tgaaaaagca gagctgttac cccactcaga agaagttata ggaccttctt
    16741 cggttaaaca cgcaagtcgt ccccagaata cctttatcga gactgatcat cctaatcttg
    16801 acttaaaaaa cagacaaaag ttatctcaaa tgctaacccg tttggaaaat gaagagcaat
    16861 cagtgtttcc cgaattggat tattttggtc agatgcacgg aacttatctc tttgctcaag
    16921 gtaaagatgg cttatttatt atcgatcagc atgctgctca agagcgggtt aaatacgaat
    16981 attatcgtga taagataggt gaggttgaca gtagtttaca gcaattgttg gtgccctatt
    17041 tgtttgagtt ttctggttct gatttcatta acttacagga gaaaatggca ctcttaaatg
    17101 aagttggtat cttcttagaa gtttatgggc acaatacttt cattttgagg gagcatccta
    17161 tctggatgaa agaagaagag attgcatctg gtgtctatga aatgtgtgac atgttacttc
    17221 taaccaatga ggtatctatt aaaacttacc gagcagagtt agctattatg atgagttgta
    17281 aacgatctat caaggcaaat catagcttgg atgattattc agcaagaaat ctgctactgc
    17341 aattggccca atgtcaaaat ccttataact gccctcatgg tagacccgta ttgattaatt
    17401 ttagtaaggc agatatggaa aaaatgttcc gtcgtatcca agaaaatcac actagcctgc
    17461 gagagctagg aaaatattag agaggatgag atgcaagagt ttttaaatct tcctaagcag
    17521 attcagctga ggcaactggt acgctttgtg accattacct taggcagtag tatttttccc
    17581 tttatggcca tgtattatac gacttacttt ggtacgtttt ggacaggcct cttaatgatg
    17641 attaccagtt tgatgggatt tgttggaact ttatacggtg ggcatctgtc agatgctctt
    17701 ggtcgcaaaa aagtcattat gattgggtca gtaggaacaa cactaggctg gtttctgact
    17761 attttagcta atttgcctaa tgcagctatt ccttggttaa cctttgtggg tattttattg
    17821 gtagagattg cttctaattt ttatggtcct gcctatgaag ctatgttaat tgatttgact
    17881 gatgagagta atcgtcgatt tgtttacacc atcaattatt ggtttatcaa tattgccgtc
    17941 atgtttggtg cagggctatc tgggcttttt tatgaccatc attttttagc cttgttagta
    18001 gccttattac tcgttaatgt actttgtttt ggcgttgctt actactattt tgatgagact
    18061 agaccagaaa cacacgcttt tgaccatggt aaaggattac tggcgagttt tcagaactac
    18121 cgtcaggtgt ttcatgatcg tgcctttgtc ttgtttacct taggtgccat cttttctggt
    18181 agtatttgga tgcagatgga taactatgtg ccagtccatt tgaaactgta ttttcagcca
    18241 acggctgtgt taggtttcca agtaactagt tctaaaatgt tatcattaat ggttttaact
    18301 aatacattgc tgattgtcct tttcatgaca gtagtaaata aattaacgga aaaatggaaa
    18361 ctattacctc agcttgtggt tggttcttta ctatttactc tagggatgct cttggcattt
    18421 acctttacgc agttctatgc tatttggcta tcagttgttt tgttaacttt tggggaaatg
    18481 ataaatgttc ctgctagtca agtcctacgt gctgatatga tggatcattc ccaaatagga
    18541 tcttatacag gttttgtgtc aatggcacaa cccctaggtg ctattttggc tagtctacta
    18601 gtatctgtca gccattttac aggtccttta ggtgtgcaat gcttatttgt agtcattgct
    18661 ttgctaggga tttattttac ggttgtttct gcaaaaatga aaaaggtgta gtatgtacga
    18721 ttatattaaa ggtcaattga ccaaaattac ggcaaaatac attgtcgttg aaacaaatgg
    18781 actaggctat attatcaatg tagccaatcc ttatagcttt acagatagtg tcaaccaact
    18841 ggtaactatt tacctgcatc aagtgattcg tgaggatgct cacttgttgt ttgggtttca
    18901 tacggaagac gaaaaagatg tttttctgaa attaatttct gtatctggca ttggccccac
    18961 aacagctctt gctattgtgg cagttgatga taatgaggga cttgtgaatg ccattgataa
    19021 cagtgacatt aagtacctaa tgaaatttcc taaaattggt aaaaaaacag cgcagcaaat
    19081 ggttcttgac ttagctggca aatttgtgga ggctccacaa gagactggta ataccaaagc
    19141 gcgcagcaat aaagcaggca atactcaact ggacgaagcg attgaagccc tccttgccct
    19201 cggttataag gcagcggaat tgaagaaaat tcgtgccttc tttgaaggaa cctctgaaac
    19261 ggccgaacaa tacatcaaat cagcccttaa actattaatg aaaggctagg tgtttcacat
    19321 gaaacgttgt tcatgggttc ctaaggataa ccagctttac tgtgattacc acgatttaga
    19381 atggggtcaa cccctacatg atgatagaga tttttttgaa ttactctgcc ttgaaagtta
    19441 ccagtcaggc ttatcctggc tgactgtttt gaaaaaacgc caagctttca gaactgtttt
    19501 tcatcattac gacattgcat cggttgcagc ctttacctcg gaggaaatgg ctgacgcttt
    19561 agagaatcca tccatcattc gccacaagct aaagctagca gcaactgtca acaatgctat
    19621 cgcagtacag aaaattcaag aagagtttgg tagtttttca acttatctct ggaattttgt
    19681 tgggggaaag ccaattaaca accttgtcaa tcaagagaat cttgttcctg ctcaaacaga
    19741 attatctatt cgcttagcta aggatttaaa gaaaagagga tttaaatttt tagggcctac
    19801 aacggtttat tcttttatgc aagcatcggg tctggtaaat gaccatgaag aggcttgtgt
    19861 tttcaataga atcttctaat tcttcgtgaa ttggaagatt ttttgataaa atgagatcag
    19921 attacgaata gataagtagg tggggaaatg aaagctgaac tgattgcagt aggtaccgaa
    19981 attttgactg gtcaaattgt gaataccaat gctcaatttc tgtcggaaaa aatggcagag
    20041 ctaggtattg atgtctattt tcaaacggct gttggggaca acgaggagcg tttactttca
    20101 gtgattacaa ctgctagtca gcgtagtgac ttggtaattt tatgtggtgg ccttggtcca
    20161 acgaaagatg atttaaccaa acaaacttta gcaaagtacc ttaggaaaga cttggtttat
    20221 gatgagcaag cttgtcagaa actagatgac ttttttgcta agcgcaagcc ttcatcacgg
    20281 acaccaaata atgagcgaca ggcacaagtg attgaagggt caatcccttt gccaaataaa
    20341 actggtcttg cggttggtgg gttcatcaca gtcgatggta ttagttatgt tgtcttaccg
    20401 ggtcctccaa gtgaattgaa gccgatggta aatgaagaat tggtaccact tctgtcaaaa
    20461 caatacagta cattgtattc aaaggtacta cgcttttttg gtattgggga aagtcagttg
    20521 gtaacagtct tgtcagattt tattgagaat caaactgatc caaccattgc tccgtatgct
    20581 aagactggcg aagtgactct tcgcttatca acaaaaactg aaaaccaagc tctggcagat
    20641 aaaaagttag gtcagctaga agcgcagcta ctatcccgaa aaactcttga aggtcaaccc
    20701 ttagctgatg tcttttatgg ctatggggag gataattcct tagcgcgtga gacatttgag
    20761 ctcttagtaa aatatgataa gacaattaca gcagcagaaa gtctaaccgc gggattattt
    20821 cagtcaactt tggcgagttt tccaggagct tctcaagtat tcaatggagg ctttgtgact
    20881 tatagcatgg aagaaaaagc gaaaatgcta ggccttcctt tagaggagtt gaaatcgcat
    20941 ggcgttgtta gtgcttatac agccgagggg atggcggagc aagcaaggtt attgactggt
    21001 gctgatattg gggtaagttt aacaggtgtt gccggaccag atatgttgga ggaacagcct
    21061 gcaggtacag ttttcattgg ccttgccact caaaataagg tagaatcaat aaaggttttg
    21121 attagcgggc gaagtcgttt ggatgtgcgc tatatcgcta ctttacatgc ctttaatatg
    21181 gtccgtaaaa ctttattaaa acttgagaat ttgctataat gttatttagt agctatagta
    21241 ggatatggtt tacgattatg tagattaatc ctgctgacga tggtttgtta aaaaagagga
    21301 gaaagtattg gcaaaaaaat taaaaaagaa tgaagaaatc actaagaagt ttggtgacga
    21361 acgtcgcaaa gcccttgatg atgctttgaa aaatattgaa aaagattttg gtaagggcgc
    21421 agtgatgcga ttaggagaac gtgcagagca aaaagttcag gttatgagtt caggaagtct
    21481 agctcttgat attgcgcttg gagctggtgg ttatcctaaa ggacgtatca tcgaaatcta
    21541 tggtccagag tcttccggta aaacgactgt ggctttacat gctgtagcac aagctcaaaa
    21601 agaaggtgga atcgcagcct ttatcgatgc cgagcatgcg cttgatccag cttatgctgc
    21661 tgcgcttggg gttaatattg atgaactcct cttgtctcaa ccagattctg gagaacaagg
    21721 acttgaaatt gcaggtaaat tgattgattc tggtgcggtt gatctggttg ttgtcgattc
    21781 agtagcagct ttagtgccac gtgctgaaat tgatggtgat attggcgata gccatgtcgg
    21841 attgcaagca cgtatgatga gtcaggctat gcgtaaatta tcagcttcta ttaataaaac
    21901 aaaaactatc gctatcttta ttaaccaatt gcgtgaaaaa gttggtgtca tgtttggaaa
    21961 tcctgaaaca acaccaggtg gtcgagcttt gaaattctat gcttctgttc ggttggatgt
    22021 gcgtggaaca actcaaatta aaggaactgg tgaccaaaaa gatagcagca ttggtaagga
    22081 gaccaaaatc aaggttgtta aaaacaaggt cgctccgcca tttaaggtag cagaagttga
    22141 aatcatgtat ggggaaggta tttctcgtac aggggagctt gtgaaaattg cttctgattt
    22201 ggacattatc caaaaagcag gtgcttggtt ctcttataat ggtgagaaga ttggccaagg
    22261 ttctgaaaat gctaagcgtt atttggccga tcatccagaa ttgtttgatg aaatcgaccg
    22321 taaagtacgt gttaaatttg gtttgcttga agaaagcgaa gaagaatctg ctatggtagt
    22381 agcatcagaa gaaatcgatg atcttgcttt agatttagat aatggtattg aaattgaaga
    22441 ttaaaaagag cccttcaggg ctttttaaaa tgtattttta aaggttagta ggactactat
    22501 tcataggtgt gtgggtgaat agaaaccatc atttaaaaag atgtgagaga gcagaactta
    22561 ggttaactga tataaaatta aaataactaa taattcaagg gaatacaggt gaaaaacctt
    22621 attttaccaa gataatatct ttaaaattaa aaaaataggt ccaaagacct atatttatag
    22681 ataagaaact gtgctacaat aaagctatca cgaaatagaa agtgagtcag caacatgatt
    22741 aaaatttaca cgatttcaag ttgtacgagc tgcaagaaag ccaaaacttg gctaaatgcc
    22801 cataagcttg cttataaaga acagaactta ggaaaagaac cgctaactaa agaagaaatt
    22861 ttagcaattt tatcaaaaac cgaaaatgga gtggagagca ttgtttcatc taaaaatcgt
    22921 tatgccaaag ctctcgattg cgatattgaa gaactaagtg ttagtgaggt tattgatttg
    22981 attcaagata acccgcgcat ccttaaaagt cccattttga ttgatgataa acgtctgcaa
    23041 gtaggctaca aagaagacga tattcgtgct tttttacctc gctctattcg taatattgaa
    23101 aatactgaag cacgattacg tgctgcactc taaaagctct tgtagagctt ttttttaaat
    23161 accttcttcg cttgaaattc ttgtttattt aatagaaata agctataata aagcatagca
    23221 cttaatttta gaaagaaggt gtaagtatgg gatttacaga tgaaacagtc cgctttaagt
    23281 tggatgatgg cgacaaacga caaattagtg aaacattaac agcagtctat cattcacttg
    23341 atgaaaaggg ttataatcca attaatcaaa ttgttggtta tgtcttgagt ggggacccag
    23401 cttatgttcc tcgttataat gatgctcgaa atcagattcg taaatatgaa cgtgatgaaa
    23461 ttgtagaaga acttgttcgc tactatttac aaggaaatgg aattgatgtt aaatgagaat
    23521 aatgggactt gatgttggct cgaaaacggt tggtgtagct attagtgatc ctcttggctt
    23581 tacagcccaa ggacttgaaa ttatcaagat tgatgaggaa aaagcagaat ttggctttac
    23641 acgtttagaa gagcttgtta agcagtacca agttgaacag tttgttattg gattgccaaa
    23701 aaatatgaat aacaccaatg gtccacgtgt tgatgcaagt ataacttatg gaaaccacat
    23761 agagcacctc tttggacttc cagtccatta tcaagatgaa cgattgacga ctgttgaggc
    23821 agagcgtatg cttattgagc aagctgacat tagtcgtggt aagcgaaaaa aagtcattga
    23881 taaattagct gcacaactca ttttacagaa ctatttaaat cggaattttt aaggagataa
    23941 aaaatgacac ataatcatga aaatgaccac caacacgaag ttatcacact tgtggatgaa
    24001 caaggaaatg aaaccttgtt tgaaatttta ttaacaattg acggtcgtga agagtttggt
    24061 aaaaattatg ttctcttggt tccagcggga tctgaagaag atgagtctgg tgaaattgaa
    24121 atccaagcct attcatttac tgaaaatgaa gatggtactg aaggggattt acaaccgatt
    24181 cctgaggatt cagatgctga atgggatatg attgaagagg tatttaatag ttttctagat
    24241 gagaactaaa ttgttagtga cactatgtta aaaagtagcc gtgagggcta tttttttaat
    24301 gttattttcg aataaataag caggaggtga taatgataaa agaattaaat gataaattta
    24361 ttaataatta taaagctaga cctttgcata gatttagaga attattacag tatgtgaaaa
    24421 tatatcagaa attgactaaa taaaagattc tgttatactt gttttataat attctttgac
    24481 gataatatca tttttcaact tactttgttt atgcactgtc actttgctat gtgcaataca
    24541 aagtcatgtt atttatctca tactaaaaaa ataatggata atagctatgt tagactagtg
    24601 atatgaagaa atattgcttt tattgaatcg ttagagaata tgatatttca aggagaaaaa
    24661 tagtgatcga gttaaacgat gaattcatcc gaaaagagac gatagaactg gccaacgatg
    24721 gcccacgagt acacaccact caatatgaaa ctaaagtacc tcggcttcat aaatgctatc
    24781 tgcttttctt tagcattatt atcagttctt taacaattgc agtacctttt ttaacagatg
    24841 cagccaatgg tcttcagtcc caaaacttat atataggaat gatgttaacg aaggggcaac
    24901 ttccctatag tgatgctttt acaacaggag ggctctttta ctttgttatc attgcattaa
    24961 gttattattt gggatcgaca ctttggctag tctttgttca ggtgttttgt ttttacttat
    25021 ctggtttata tctttataaa ctcattaatt atatgacatg ctttcaaaaa gtggctttaa
    25081 ctttttcaat tagctactat ttgttgtctg ttagtcttgg ttttggagga ttgtatccta
    25141 ctcaactggc tatgccattt atattaatat cggcttggtt tttaactaag tattttgcct
    25201 gtttagtgaa agatgaggca tttattcttt ttggctttgt aggtgctctt gcaatgctaa
    25261 ttgacccgag tacccttatc ttttggtctt ttgcttgtgt gacagttttt tcttataata
    25321 taagccaaaa gcatcttgca agaggttttt atcaactgct agcttcgatt tttgggatga
    25381 ttttagtttt ttacacagca ggatatttca ttttgaactt acaagtgcta aatccttatt
    25441 tatcacaaac gatgatttat ccttttactt tttttaaatc aggaaactta tcgttgcttt
    25501 ttggactggc tattcagttg ttcttcgc
//
LOCUS       DAUVUY010000015        24692 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.015, whole genome shotgun sequence.
ACCESSION   DAUVUY010000015 DAUVUY010000000
VERSION     DAUVUY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24692
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.015"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1152
                     /locus_tag="VOX58_000602"
     CDS             <1..1152
                     /locus_tag="VOX58_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HES5877853.1"
                     /translation="TSLKDFSGAKVTAQQGVWHVNLLTQLKGAKLQTPMGDFSQMRQA
                     LTSGVIDAYISERPEAMTAEAADSRLKMITLKKGFAVAESDAAIAVGMKKNDDRMATV
                     NQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKASFLGQMWAILKGNWKQFLRGTGMT
                     LLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALGQKLFGWLLTIYIEIFRGTPMIVQ
                     SMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMSEIVRGGIFAVDKGQFEAATALGF
                     THGQTMRKIVLPQVVRNILPATGNEFVINIKDTSVLNVISVVELYFSGNTVATQTYQY
                     FQTFTIIAIIYFVLTFTVTRILRHIERRFDADTYTTGANQMQIAEVSNV"
     gene            1145..1885
                     /locus_tag="VOX58_000603"
     CDS             1145..1885
                     /locus_tag="VOX58_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HES5877854.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDHIIFMDKGL
                     ITEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            2027..2143
                     /locus_tag="VOX58_000604"
     CDS             2027..2143
                     /locus_tag="VOX58_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HES5877855.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(2369..3379)
                     /gene="gap"
                     /locus_tag="VOX58_000605"
     CDS             complement(2369..3379)
                     /gene="gap"
                     /locus_tag="VOX58_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HES5877856.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(3727..5805)
                     /gene="fusA"
                     /locus_tag="VOX58_000606"
     CDS             complement(3727..5805)
                     /gene="fusA"
                     /locus_tag="VOX58_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HES5877857.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(6172..6642)
                     /gene="rpsG"
                     /locus_tag="VOX58_000607"
     CDS             complement(6172..6642)
                     /gene="rpsG"
                     /locus_tag="VOX58_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HES5877858.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(6663..7076)
                     /gene="rpsL"
                     /locus_tag="VOX58_000608"
     CDS             complement(6663..7076)
                     /gene="rpsL"
                     /locus_tag="VOX58_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HES5877859.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(7284..9905)
                     /locus_tag="VOX58_000609"
     CDS             complement(7284..9905)
                     /locus_tag="VOX58_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HES5877860.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKV
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEVLAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQETLAALQAKQSSLEATIATTEHQLTLLKTLANEKGYRHLDED
                     IATVPDLQVAPPLTGVKPLSYSKIDTTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESK"
     gene            complement(9896..10735)
                     /gene="purR"
                     /locus_tag="VOX58_000610"
     CDS             complement(9896..10735)
                     /gene="purR"
                     /locus_tag="VOX58_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HES5877861.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            complement(11002..11940)
                     /locus_tag="VOX58_000611"
     CDS             complement(11002..11940)
                     /locus_tag="VOX58_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HES5877862.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVTEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            complement(11930..13201)
                     /locus_tag="VOX58_000612"
     CDS             complement(11930..13201)
                     /locus_tag="VOX58_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HES5877863.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     ITYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEEINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQTTKSLLNMPLLEEENNEN"
     gene            complement(13203..13835)
                     /locus_tag="VOX58_000613"
     CDS             complement(13203..13835)
                     /locus_tag="VOX58_000613"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HES5877864.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVSQKAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            complement(13828..14490)
                     /locus_tag="VOX58_000614"
     CDS             complement(13828..14490)
                     /locus_tag="VOX58_000614"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HES5877865.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGVDIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            complement(14500..15372)
                     /gene="rsgA"
                     /locus_tag="VOX58_000615"
     CDS             complement(14500..15372)
                     /gene="rsgA"
                     /locus_tag="VOX58_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HES5877866.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(15796..16668)
                     /gene="rsmA"
                     /locus_tag="VOX58_000616"
     CDS             complement(15796..16668)
                     /gene="rsmA"
                     /locus_tag="VOX58_000616"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HES5877867.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKD
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKTKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            complement(16782..17372)
                     /gene="rnmV"
                     /locus_tag="VOX58_000617"
     CDS             complement(16782..17372)
                     /gene="rnmV"
                     /locus_tag="VOX58_000617"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HES5877868.1"
                     /translation="MQRGYLSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHANFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            complement(17344..18135)
                     /locus_tag="VOX58_000618"
     CDS             complement(17344..18135)
                     /locus_tag="VOX58_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HES5877869.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALALADQYPNVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKETTANAKRVLKLD"
     gene            18420..19250
                     /locus_tag="VOX58_000619"
     CDS             18420..19250
                     /locus_tag="VOX58_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HES5877870.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNGL
                     "
     gene            complement(19361..20299)
                     /locus_tag="VOX58_000620"
     CDS             complement(19361..20299)
                     /locus_tag="VOX58_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HES5877871.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMVQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITLEKIENNLASS"
     gene            complement(20321..21235)
                     /locus_tag="VOX58_000621"
     CDS             complement(20321..21235)
                     /locus_tag="VOX58_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HES5877872.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIVDKDLEKAKTLQYTINETIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            complement(21247..21909)
                     /locus_tag="VOX58_000622"
     CDS             complement(21247..21909)
                     /locus_tag="VOX58_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HES5877873.1"
                     /translation="MTSKKQGLLHSLFKLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSHFKHYFKSGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELPLLILFKRSLLLAGILFP
                     WTFAFLVVIGLLVFTLPLTLLTLFCGLSLLATMGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            complement(22066..22896)
                     /locus_tag="VOX58_000623"
     CDS             complement(22066..22896)
                     /locus_tag="VOX58_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HES5877874.1"
                     /translation="MTKKKLTASDVLTTVILCILTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(22909..23796)
                     /locus_tag="VOX58_000624"
     CDS             complement(22909..23796)
                     /locus_tag="VOX58_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HES5877875.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLINTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIAILLTTSVGQPIILYIAAMGNIDNSLMEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            complement(23899..>24692)
                     /locus_tag="VOX58_000625"
     CDS             complement(23899..>24692)
                     /locus_tag="VOX58_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HES5877876.1"
                     /translation="LDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPRAF
                     FANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFANG
                     QTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDEQK
                     VAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPYYN
                     TIDGFAEMRTLWFPMVQAVSNGDEKPEDALKAFTEKANKTIKKTQ"
BASE COUNT         7254 a         5301 c         4661 g         7476 t
ORIGIN      
        1 acaagcctca aggatttttc tggagctaaa gtaactgccc agcaaggcgt ttggcatgtc
       61 aatctcttaa ctcaactaaa aggtgctaag ttacaaacac caatgggaga tttctctcaa
      121 atgcgacaag cccttacttc gggtgttatc gatgcctata tttctgaacg gcctgaagcc
      181 atgactgctg aagctgctga tagccgtttg aaaatgatca ctcttaaaaa agggtttgct
      241 gttgctgaat cagatgctgc tatcgctgtc ggaatgaaaa aaaatgacga tcgtatggca
      301 actgtcaacc aagtgcttga aggattttct caaacggatc gtatggccct gatggatgat
      361 atggttacca aacaacccgt ggaaaagaaa gccgaagatg ctaaagcatc atttctaggc
      421 caaatgtggg ctattttaaa aggtaactgg aagcaattct tacgtggaac tggaatgacc
      481 cttctgattt ccatggtcgg aaccattaca ggtctcttta ttggattatt aatcggtatt
      541 ttccgtacag ctcctaaagc taagcataaa gtagctgcct tgggacaaaa actctttggt
      601 tggttactca ctatttacat cgaaatcttc cgtgggacac ctatgattgt tcaatctatg
      661 gttatttact acggaacagc gcaagccttt ggtatttcga tcgaccgtac cctagcggct
      721 atttttatcg tatctatcaa tacgggtgcc tatatgagtg aaattgttcg cggtggtatt
      781 ttcgctgtcg acaaaggtca attcgaagca gcaactgctc ttggctttac tcacggacaa
      841 accatgcgta aaatcgtgct accacaagtt gttcgcaaca ttttaccagc aacaggtaat
      901 gagtttgtca tcaatatcaa agatacttct gttttgaatg ttatctctgt tgtggaactt
      961 tacttctcag gtaataccgt agccacacaa acctaccaat atttccaaac ctttacgatt
     1021 atcgccatta tctactttgt tcttaccttc acggtgacac gtattcttcg tcatattgaa
     1081 cgccgtttcg atgccgatac ttacaccact ggagcaaacc aaatgcagat tgcggaggtc
     1141 tcaaatgtct aactcaatta ttgaaatcaa aaacctcaaa aaatcttacg gcagcaatga
     1201 agtactcaaa gatatctccc tttctgttaa caagggagaa gtgatttcca ttatcggttc
     1261 ttctggttct ggaaaatcaa ccctcctgcg ctcgatcaat cttttggaag agccaagtgc
     1321 cggtcaaatc ctctttcatg gtgaagatgt ccttgctgaa cactacaact tgactcacta
     1381 ccgtgaaaaa ctaggcatgg ttttccaatc ctttaacttg tttgaaaacc taaatgtatt
     1441 ggaaaatgct atcgtcgctc aaaccaccgt cctcaaaaga gaccgtgcac aagccgaaca
     1501 aatcgctaaa gaaaacctca atgcggttgg tatgacagaa caatactggc aagccaaacc
     1561 aaaacaatta tcaggggggc aaaaacaacg ggttgccatt gccagagcct tatctgtcaa
     1621 tccagaagcc atgctctttg atgaaccaac atcagctctt gatcctgaaa tggttggtga
     1681 ggtccttaaa accatgcaag acttggcaaa atcagggttg accatgatta ttgttaccca
     1741 tgaaatggaa tttgctcgtg atgtttctga ccatatcatc ttcatggata agggattgat
     1801 tactgaagaa ggaagtcctc aacaaatttt cgagaatcct acacaagatc gtaccaaaga
     1861 atttttacaa cgcttcttaa aataagataa aggatccaac tatttctatg ccagttgggt
     1921 cttttttcaa ttcaaaaaga atttgtcata aaatacttta ccagttaact attttatgct
     1981 atacttattt ttgttaacgt taataattta tatttaggag gataatatgg cagaaaaaac
     2041 tgacttatcg tcagcttaca gaagactgaa aagtcccaat attaaaacca gaaaacgtgc
     2101 tctgaagatt attcacgagt acaaacgtta cggtaaaaaa taagatttta ctttttatct
     2161 ctccaaattt aagtcagaca aaaaaaggct aagacacccc ttccatctta gcctttttgt
     2221 atacagttta ttaagggaac tattttaaaa tatcttttat gctagagtct agaatagttc
     2281 aaaacgttaa aatcatacat ttaaggtagt gtttaaagta tacgaaaaag aactcagcat
     2341 aaaccaagct ctctttcgtt ataactaatt atttagcaat ttttgcgaag tactcaagag
     2401 tacgtacaag ttgagcagtg taagacattt cgttgtcata ccatgataca actttaacca
     2461 attgtgatcc gtcaacttcc attactttag tttgagttgc gtcaaacaat gaaccgtatg
     2521 atacgcctac gatatctgaa gaaacgattg gatcttcagt gtaaccgaag ctatcgtttg
     2581 aagcagcttt catagcagcg ttgatttcgt caacagaaac gtttttgtca agagttacaa
     2641 ccaactcagt tactgatcca gttggaacag gaacacgttg tgcagcaccg tcaagtttac
     2701 cgttaagttc tgggataaca agaccgatag ctttagcagc accagttgag ttaggaacga
     2761 tatttgcagc accagcgcgt gcacgacgaa ggtcaccacc acggtgtggt ccgtcaagga
     2821 tcatttggtc accagtgtaa gcgtggattg tagtcataag acctttttga ataccgaatg
     2881 catcgtgaag agctttagcc ataggagcta aacagtttgt agtacatgaa gcacctgaga
     2941 taactgtttc agtaccgtca agaatgtcgt ggttagtgtt gaaaacaact gttttaacat
     3001 cgtttccacc aggagctgtg ataacaactt ttttagcacc gttagcatgt aagtgttttt
     3061 cagctgcttc ttttttagca aagaaaccag ttgcttcaag aacgatttca accccatcag
     3121 ttgcccagtc gatgttttct ggatcacgtt cagcagaaac tttgatgaag tttccgttta
     3181 cttcaaatcc accttcttta acttcaactg ttccatcaaa acgaccttga gttgtatcgt
     3241 atttcaacaa gtgtgcaagc atatttggat ctgtaaggtc attgatacga gttacttcaa
     3301 caccttcgat gttttgaata cggcggaatg caagacgtcc gatacgaccg aaaccattaa
     3361 taccaacttt aactaccatt agtgatttcc tccttatgaa aatcaaaaga atattattta
     3421 gagggaatgc tccctaccta ttgtgaaaag attaacttac agtcatctgc aacaaccttt
     3481 caacagaact attatataac tatttcagaa aaaatgcaaa taaattagcc attttcataa
     3541 agggcagctt ctttcttcta gccttcaaaa cccaccaaat ataggaaagg gctttctttt
     3601 ttctatgttc tattctgtaa tttatctttt tattgggaaa gatttccaaa acttcaaact
     3661 gtactataac gttctgttac actaaaaaag aggttaggat agttatcctc aacctcttag
     3721 tggtggttat tcacctttgt ttttcttaat gatttcttct tgaactgact taggaacatc
     3781 ttcatagtgg tcaaatacca tcatgaatgt accacgtcct tgagtagcag aacgaagaac
     3841 tgttgcataa ccgaacattt cagcaagtgg aacataagca cgaacgattt ggctgttacc
     3901 atgtgcttcc ataccgtcaa cgcgtccacg acgagctgtc acgtgtccca taacgtctcc
     3961 aagattgtct tctggagctg taatcgttac aagcatcatc ggctcaagga tagctggttg
     4021 tgcagattta gctgcttctt taagtgcaag tgatgcagcg atcttgaagg cagtttcaga
     4081 tgagtcgaca tcgtggtatg aaccatcgta aagcttagct ttaacatcaa ccattgggta
     4141 acctgcaaga acaccgtttg ccatagattc gatcaaccct ttttcaacag ctgggataaa
     4201 ttcacgagga actacaccac caacgatagc attttcgaat tcgaaacctt taccttcttc
     4261 atttggtgta aattcaatcc aaacatcacc gaattgacct ttaccaccag attggcgttt
     4321 gaagaatcca cgagcttgtg tagaagcacg gaatgtttca cggtaagata cttgaggagc
     4381 accaacgtta gcttcaactt tgaactcacg tttcatacgg tcaacaagga catcaaggtg
     4441 aagttcaccc ataccagcga taactgtttc accagtttca acgtttgttt caacgcggaa
     4501 tgttggatct tcttcagcaa gtttttgaag ggcaacaccc atcttgtctt ggtctgcttt
     4561 agattttggc tcaaccatca actggataac tggttctgga acttcgattg actcaagaat
     4621 aacttttgct ttttcatcag tcaatgagtc accagttgtt gtgtctttca aaccaacagc
     4681 ggcagcgatg tcaccagcgt aaactgtttc aatttcttga cggctgtttg cgtgcatttg
     4741 aaggatacgt ccgatacgct cacgtttacc ttttgatgtg ttcataacgt aagaaccgct
     4801 attaagaaca cctgagtaaa cacggaagaa tgtcaaacga cctacgaatg ggtcagtcat
     4861 gatcttgaag gcaagagctg caaatggctc ttcatcagat gctggacgtt cttcttcagc
     4921 atctgtatcc gggttaacac ctttgattgc agggatgtca agtgggcttg gaaggtatgc
     4981 gataaccgca tcaagcatca attgaacacc tttgtttttg aaggcagaac cacaaagtac
     5041 tgggaagaat tcaacgttga ttgttgcttt acggatacca gcaatcaatt cgtcgttagt
     5101 gatttcttca ccttcaaggt atttcatcat caagtcttca tcagtttcag caactgcttc
     5161 gattaatttt tcacggtatt cttgagcttg ttctaagtat tcttctggaa tatcttcttc
     5221 aaggatatct gtaccaaggt cgttggtata gatttctgct ttcatcttga tcaagtcaat
     5281 gataccacgg aaatcatctt ctgcaccgat tggcaattgg attgggtgtg catttgcttg
     5341 aagacggtca tgaagggttt gtactgagta aaggaagtca gcaccgattt tatccatttt
     5401 gttggcgaaa acgatacgtg gaacaccgta ttcagttgct tgacgccaaa ctgtttcagt
     5461 ttgaggctca acacctgatt gtgagtcaag aacggttaca gcaccatcaa gaacacggag
     5521 agaacgttgt acttcgatgg tgaagtccac gtgtcctggt gtgtcgatga tgttaacgcg
     5581 gtgaccatcc cattgagctg ttgtagcggc agaagtaata gtgataccac gttcttgctc
     5641 ttgctccatc cagtccattt gtgatgcgcc ttcgtgtgtt tcaccgattt tatgaatttt
     5701 accagtgtag taaaggatac gctctgttgt agttgtttta ccagcatcga cgtgagccat
     5761 gataccgata ttacgagttt ttgcaagtga aaattcgcga gccatgtgtt tctcctattt
     5821 ttttagttta ctctttcatt atagcactat ttagcaaaaa cggataggca ggacctaccc
     5881 gttatctaaa tatttgttgg taaagataat acctattgcc tttataaggc ttggtatctt
     5941 attcattgaa ctcaggctaa acgctgtgtg aaaaagataa actttcctag agacaaaggt
     6001 ttcttcgtca aatttcctat tttcactttg cgttctatac gccttcgtat cttattaatt
     6061 gagttcgagc tacaagccac tgacaaaaag ataatagttc ctagaaactg aagtttctgc
     6121 gtcaatttcc ctattttgtc tagcatttgt ttgacgctaa aatatcttat attaccaacg
     6181 gaagtgagca aaggcacggt tagcttcagc cattttgtga gtgtcttcac gtttcttaac
     6241 tgatgcacct gtgttgtttg cagcatccat gatttcttta gcaagacgat ctttcatagt
     6301 gtgttcacca cgagcacgtg atgcgtttac caaccaacga agtccaagtg ttgtacgacg
     6361 ttctggacga acttcaactg ggacttggta gttagaacca ccgacacggc gtgcgcgtac
     6421 ttcaagtaca ggcatgatgt tgtccatagc tgtttcaaat acttcaagag cgtcatttcc
     6481 tgttgcttct ttgatagcgc tgaaagcatc gtaaacgata gtagcagctg taccacgttt
     6541 accgtcaagc ataacacggt tgataagacg tgttacgatt tttgagttat ataatggatc
     6601 tggtaatact tcgcgtttag gcgcttgatt tttacgactc attttctctt atcccccttc
     6661 tattatcctt ttggacgttt cgcaccgtat ttagaacggc cttgtttacg gtcagcaaca
     6721 cctgcagtat caagtgcgcc acgaacgata tggtaacgta cccctggaag gtcttttaca
     6781 cgtccgccac ggataagaac aacgctgtgc tcttgcaagt tgtgtccgat acctgggatg
     6841 taggcagtta cttcgataag gttgctcaaa cgtacacgag cgaatttacg aagggctgag
     6901 ttaggttttt taggtgtcat tgttccaaca cgagtagcaa cgccacgttt ttgtggggca
     6961 gccattttag tttgaacttt tttgtgactg ttgtaaccaa tgttcaaagc tggtgaatct
     7021 gatttttcaa ttttagattt acgtggttta cgtaccaact ggttaattgt aggcatctac
     7081 attctcctgt gtttttttat ttttggttga tatagcactt ggtgacagcc catatccggt
     7141 gcactttttg caacaattgt cagcacgtct ctgtacactt ttgagagacc aaaagtaaaa
     7201 agtaccgtct aatataataa cacagacggt acttcatgtc aaatagaatt tttcctttga
     7261 tgcaattgct tatgacgaat gcatcactta gattccttac ggaacctaaa tcctgtgagg
     7321 ccgacagctg ccaacatgac gactcctgct ttaagagctc tttgagtact ttcatcaaca
     7381 tcagaaatga gattgctcgt tgtagaagat cctgagccat aagatgtctt agatgagggc
     7441 tgagtaatcg aagatgtgat tttagacaca atggcattac ttgctaccat ttcagaggtt
     7501 tggccaacaa gcgcttctgc tacaagactt gtattttcag cagctaatct tgctgaagct
     7561 tctaatagtt gtttcgtttc tttaaccatt tcttgaacaa gcggagtagt atctatctta
     7621 ctatatgata gcggttttac gcccgtaaga ggtggagcta cttgcaaatc aggcacagta
     7681 gctatatctt cgtctaaatg gcgatatccc ttttcattag ctaaggtttt aagcaaagtc
     7741 aactggtgtt ctgtggtagc aatagtagct tctagactgc tttgtttagc ttgtaaggct
     7801 gctaaagttt cttgtgcatt taacaaagat gaggtagttt tagctaaatc ttgcttagta
     7861 ttatcaatgc gctcacgtat cacttgaagg cggttaggat tcaatttaaa gtcccttaga
     7921 tattgcaaat gagctttttt agccaccagt gctgtcactc tggctgcggc ttgctctgct
     7981 aagacttctg tctggtgcag tgcggctttc aacgatgcta gcttagctaa tgattgatca
     8041 cgagtagctt cgagttgtgc ttgttttgct ttagctgcta gtaattctgt tctcaaagaa
     8101 cctttagtat catttaattg tctcacttgg aggtttaagc tgtctgactg cttaagtgtg
     8161 cttgctaatt taccttgaag ttgacttact ttagcttggg ctgccataat gtcagcttct
     8221 tgatgaatag ccgacaaacg attttctaat gagcttactt ttcctttgat cgctgcaata
     8281 gtatcagata cagtgcctac tctttgggca taatcttttg tacttccaac ggcttttata
     8341 ggggtcttat taaagcgttg atggttagca atgttagact ctggaaacat tacaaagtgt
     8401 tcattcaaag atcctacatt gctggttgaa aatccaaggt aaacaggcgc attagggtta
     8461 cgtttatcta cacgtaaaaa gttaatagca tggccgtatg tatttccgtg taaatgatct
     8521 gtaaagagca tatacttgat actgtcataa ataccacgtt taataccatt cacagtatgc
     8581 acatcgttaa aagcaccgat attctcgtac atgttatcat catttcgaat gagccctgac
     8641 gctccggcag agtcttcaat aatagtttta tcatgaggcc caacaccata atgccctgat
     8701 acccctggct gtccgtagac aaatgatggt cttgtattac catgagtttt cttatagctg
     8761 gtactaagta atcttgcaaa ttcttgtgat cctgctgtaa cagtaactgg tggtagacct
     8821 aattgtcttc ttacactatt aatcatgtga gctgcaaact gcgctagctc attttgcact
     8881 tctggtgtca aattatcggg atcaacaaag cgattacgat ctgctggaat atcttggtat
     8941 tgatttaatt gattacctgg actagctttg gcaataattt gatctgcatg ctctttgtaa
     9001 taattattgt aactagctga tccaatataa ccactagctt ctaatttttt aagttcttca
     9061 agaggatagc cttgcggtac tttcatggta ttattaccca caatgctatc ttgagtagac
     9121 ggagctgagg atttaagacg actaagttct gcctcttttt ctgctagagc agctttctga
     9181 gctgccaact cttcagtcaa ttgcttttta actttagctt tttgattttc taagtcagct
     9241 ttagccttct ccaattctga gcttaatgct tttgtattat cattagccgt ttgagctgct
     9301 ttagtgatag catcaggatt gctaatcata gcattgagct tagcaatatt ttgttcagac
     9361 gttttgactt gttccactaa atcttgagct cgagtagttt ctgctgaaat gctagctttt
     9421 tgttctgaca atgcagtctc ttttgaatgt tgatctgctt gagcattatg aagctctgtt
     9481 tcagtagctg ttaactctct ttgatgttcg gctccttggg ctaatagcgt ctcctcacta
     9541 cttgcaagag tattagtgta aatttcttgt gcagaggtta aagctttttg ttcattatct
     9601 tgctgctctt ttaagtggtt gatttcagca gtagtttttg tcagagcggt agcaagctct
     9661 gtcagttctg ctttttgttg actgagagtt ttttcaactg catcaatagt tgcctttgcc
     9721 tcttgaattg tttctggttt tggtaaacta tcgtcgtgag tattactcgc cttcgtttct
     9781 cctgaggctc tatcatctgc tttgacttgg tgagatacgc ctacactggc actcaataaa
     9841 gcaattgttg aggttaaagc aattttctgc ttaacttggt ttggcttcgt ttgttctaag
     9901 tccattctct ctcctttttc catttatcga aaatattacc aacttctaca acaacattgt
     9961 tgtttttgac atcaatctct gaaactttca gcaatgattt gaaggtcatt tgctcacgct
    10021 ctgattgagc gttttctgca aacacagcaa caccaactaa agtgctgtca aattctgtca
    10081 agagactgat cattcctgta atcgtccccc cgcccttcaa gaaatcatct acaatcaata
    10141 cacgactatt gggtttcaaa ctacgctttg acagaaacat cttttcaata cgatcactag
    10201 aagcactcgc ataattgaca gacactgtcg atccttctgt tatttttaaa tcacgtctaa
    10261 caatgacaaa aggaacactt aaaatattag ccactgcatt tgctaaagga actcctttgg
    10321 ttgcaactgt catcacagcg tcaatctttt ctcctttaaa ggcattggca ataatacgac
    10381 caatattttg taaaatttta ggtgtactca gcaagtctga caaatagata tagcctccag
    10441 gtaagatgcg atcactctct gataaccttt gacacaggtc ttcaacaatg gtacgcgctt
    10501 ctgtttctga aatactgggg gtaaaaatta cgcctccact tgctcctgta agtgtatcga
    10561 tatcaccaat gttagcttct tcaaaagctt tcttgataat agcgatatct tctgagatag
    10621 aagattttgc ggcctcatat ttagttgcaa aggtgtttaa actagttaat ttatacggat
    10681 tgttaatcaa ataatttgaa atgacaacca tacgttcact tcgtcttaat ttcatagttt
    10741 ccccattttt atgtactttt tgctattata ccataaagtt aataaaaaac gaacattcga
    10801 ttaggaaaat attggttttt ttgttcttat taatatcatg caagaattca atctaagata
    10861 aactaatcaa cattcctact gaaaggacat tctacttaat aaccataaca agagccaaaa
    10921 acacttatga aaccatcata agtgttaagc acatcaaaaa tcatcacaaa agcaaagtaa
    10981 ctattacttg agaaacactg atcaatagtt tggcttgtaa aaagaccgat tatccatggc
    11041 aaaaatccga ttggtcatct cgccctcgct aacacggcta agagctgttg tcatcatcat
    11101 catattagca tcaatattgt caatcatatg aataatttca gcttccatga tgcgtggacg
    11161 taccggacta ccgtattcta attgcccgtg atggcttaaa atcacgtgcc taaggacaat
    11221 gacttcttct ttggtatcat cgatttgtag ttctgaaatt acttttgtaa tttcttcgtt
    11281 aatgagagaa atatgcccaa tcaagttacc acgaacggtg tattctgtat tatctggtcc
    11341 tgttaattct atgactttag ctaagtcatg taacataata cctgcaaaca gtagactctt
    11401 atttaagtca gggtagatat ctccaatgct atctgctagt ctgaccatcg tagcagtatg
    11461 gtaggcgagg cctgactcaa aagcatggtg gtttgtttta gctgcaggat aagtataaaa
    11521 ttctttatca tacttacgat aaagagccct tacaattcgt tgccacgttg cattttcaat
    11581 tttaaagagc atctgttcca aatagtcacg tacttctgtc acactgacag gtgccttttc
    11641 tttgaagtcc tttggatcat tgggctcacc tgggcgaaca tttcgcaagg tgatttgatt
    11701 aacctgaggg gtaccattat acacctcgcg tcgccctttc ataaacacga ctttaccagc
    11761 agtaaattct tcaacgttat aaggttgggc atcccacaga tttcctgaaa tctcgccagt
    11821 gtcatcctga aaggtaaggc taataaaatc tttaccagct cttgtcttac gaacctctgc
    11881 cgacttaatg aggtagaacc cttcaaaaag ctggtctttt ttcatttgat taattttcat
    11941 tattttcctc ttctaataag ggcatgttca agagagattt tgttgtttgg tcttgataag
    12001 tttcaacggt attcaaggct cgaacaatgg catttgtcct tgttgaaatg agctgatcca
    12061 gcgtattatt agctgtattc atttgctttt gagccttggc aagcaggcca ccaaatttat
    12121 cgaattctaa cttgacattg cccaaaattt tactgatgtc atcagcattt ttttggatat
    12181 taagggtctt gaaaccaaca gataaggaat tgagcaaagc agacagggtc gaaggacctg
    12241 caaccacaat attttcttcc cgacgaaggc tatcaaagaa agccgcattt ctgaccactt
    12301 ctgaataaag accttctgtt ggtaagaaca taactccgaa attggtcgtc tctggggggt
    12361 tcaagtactt tttatgaatg tctttggcaa agcgtttgat agctgccaga agtgcttttc
    12421 ggctagcctc gatggccagt ttatcaccaa cttcgtaagc atcttctaat cggtaataat
    12481 cttcaagagg gaattttgag tcaatcggta ggtaaatata accgccttga ccatttcctg
    12541 ggagcttaat cgcatattct acgcgttcac tagaaccact aaccgttaca aattctcttt
    12601 cgtactggct tgatgtcatg atatcctcaa tgatttggcc tagttgaagt tcgcctaaaa
    12661 tgcctcgtgt tttggtattg gacaaaacct tatttaaagt acccacatct tgagccacgc
    12721 tacgcatttc tcccaagcct ttattgacac tttctagttg cttggataca gaatcgaaag
    12781 aggcgtgcag acgatttttt aaggtttctt ccaatttttc ttcaacgatc tggcgcattt
    12841 cctccaaacg tttttcgtta gattcttgca tatttttgag cgattggttg acctgctggt
    12901 taatttcttc taagcgtttg tcagaccggt ccctactatc agacaaacta cggtgcaaga
    12961 cgtcacgaat atctgttaat tgttggtaaa ggtctgcttg ttgtcggttc atcagctgtg
    13021 ttagctctaa caattgttgc ttgttagctg tatccaactg gtaggttatt tggtcagaca
    13081 aattatccgc gttgccttct agggtttggg caagctgatg ttgaaggccg ttgactttga
    13141 acaacagata agcccctaaa cctaagagaa ccaagaccaa aaggaacaag ataaggtcca
    13201 tactaactcc aatctttact ttgaataata atcaagtagc cacttggcaa actaactgta
    13261 ataggttgat ggataaaggc attactggaa tagattttct ttttgaaaaa attatcttgc
    13321 gtcaactcaa atttagcacc agtaatggtc aagtcagctt ctccttctgc cataaatgcc
    13381 acataggtca tcccttcttc ttgatggata aggtgctgac ctgccgggcg gtaggtaatc
    13441 atattttgtt ggtcacgcaa ggcgatttgt gccataaagg gagcaatacc agggtcgcta
    13501 ggcaagaaaa tattggataa gaggtggtcc attcgacccc caaaagcccc aaaaatggtc
    13561 acttccgcct caggaaaacg agcaaagacc tctttaaggg ctaattccgt atccgtatca
    13621 tttttttctg gatgagcagt aataaaaagc tctgccttct cttttatatc cgtaaacgct
    13681 ttttgggaaa ccgaatcaaa atcaccaaca gccatattca aaggaagccc attttctaat
    13741 aaaaataggc tccctctatc aataccgaca aaataatcaa aatcacggct gatataagag
    13801 agatctcctc ccgcaaaaag agcaacttta gacattcaag gccgtcctta aggtttggac
    13861 ttggctcact aggtcactgg ccttgaaaag gtaagaacct gccacaaaga cattggcccc
    13921 tgcttcataa caagcacgaa tggtcttatt gtctactccg ccatcaacct caatgtcaaa
    13981 ggacaatcct ttttcatctc gccatttggc tacggtagcc actttttcta aacattctgg
    14041 gataaaggcc tgacccccaa aacctgggtt gactgtcata atgaggactt ggtcaactaa
    14101 gtctagcaaa ggttctaggg ctgttgcagg agttcctgga ttaatcacta cccctgcttt
    14161 catgccagct gccttgattt tttgaagggc tccatgaata tgacgagtac tttctgtgtg
    14221 aatggtcatg atatcaacac cagcttgagc aaaagcctca acatagcgtt caggatcaac
    14281 caccattaga tggcaatcaa agaccaattt actgtgcttt ctcatacttg ccacaacatc
    14341 tgcaccaaaa ctgatgttag gaacaaattg accatccata atatcaatgt gaacgtattc
    14401 ggcatctgtt tcttcaatac gagctagctc agaagcaaaa ttagcataat cagctgctag
    14461 aatagatggg gcaattttta gagttgacat ggagtagacc tactttcttt tgataacttt
    14521 tttatatgtt tcacgacggt tttcaatttc actgagaaat tgcaggtaat gctcataacg
    14581 aactggccac aactctcccg tttcaagcgc tgccttgaca gcacacttgg gttcgtgggt
    14641 atgggtacag gatctaaact tacactcatg gctcagccgg cgcagctctg gaaaagcctc
    14701 gttcaaatct tcagcattag caatatcata gtctaatgat gaaaaacctg gcgtatccgc
    14761 gatttttccg ccatgggtat tgtaaaaact cacagctcgg gtggtgtgac gaccacgccc
    14821 tagactatct gagatttctc ctgtttcaag agccaattcg ggggcgattc ggttgagaag
    14881 ggttgatttc ccaacacctg tttgtcccat aaagacggta attttatcag ctagcaaagg
    14941 caacaattcc tcaagactgg tcacaaaatc gtaaccgatt gcctgatact gtcgaccaat
    15001 agccttaatc tcctcgggac tatctaataa gtccatctta gagatataaa ccactggatg
    15061 aatggctttg tgttcaagca agatgagaaa gcggtctaga agattgctgt taaattctgg
    15121 ttctttagca gacataataa ccactgcttg gtcaatattg acgataggag gtcttaccaa
    15181 actgttttta cgaggatgaa tcgctaaaat ataaccttca gagttgtcct ctgctgagaa
    15241 atccacgata tctccaacat aaggtgtctc accgcgcttc ctaaaattac cacgcgctcg
    15301 cgtctgatat acctgtcctt ctgactccac gtaataaaaa ccagccagag atttaatgat
    15361 tttaccttgc aactgtgctc ctttgcccta ctataataac tctattatat cagaaaaccg
    15421 ctcccaaatc agcatttgac tctaagtttc actcgcctta aaaaactgtc aagattctct
    15481 agaccactgg ctatcattta ggtcactgct ttttaaaaat agaatatttt atcaactaaa
    15541 aatcagatca ctgatgattt ttgaggtagg aaacgatatg aattttaaga agacctttgc
    15601 tatttacgaa aaagagtcta accgtaccgc tatagtcacg ctcttgttaa ttgccccttt
    15661 tttccggggt attaaaaaaa ctagctctgc actgcgatac cttaacggcc agcttcaata
    15721 agaaaaatcc ccctgaactg aacatttctg ttttgtctac aagggaattt ttttattgtc
    15781 accgggagaa agcttttata atccaacttc ttttaaagca tcagctaatt tgccaaaatc
    15841 ttgaatcgac aaggcctcac cacgaataga aggcttgata tctgctagtg ctagaccttt
    15901 ttcaagttta gtcttgatgt cttcagactt cccaaaatgg ctagtcaagt tattccacag
    15961 ggtcttgcga cgatggacaa aactgagacg agatactctg aaaaagaaat cttcgtcctt
    16021 aacctcaatc aaaggctggt cgcgacgcac cattttgagg atagcactat cgacatttgg
    16081 tgcaggtaca aagacggtgc gcggcacaat gaaggcaacc ttggcagtca tgtaatactg
    16141 caccgcaatg gacaaggaac cataagcttt agtgtttggc tcagccgaga tacgatctgc
    16201 cacttcacgc tgcatcatga ctacaaactc ttggaatgga attttgctct caatcaagtg
    16261 catgagaata ggcgttgtga tgtagtaagg tagattagcc accaccttaa tgggcaaatc
    16321 aggattttta aactgcttga tttgggtctg caaatcagct tttaaaatat cttgattgac
    16381 cacttgcaca ttatcaaaat cgcgcaaggt atctgccaaa atcggcacca agcggtcatc
    16441 aatctcaaaa gccatcactt cagcagcatt ttctgccaaa aattccgtca aagccccaat
    16501 tccaggacca atctcgataa cattgacatt ctgatcaatc tcagccgtgt caacgatttt
    16561 ctgtaaaata ttggtatcgg tcaaaaagtt ctgaccaaaa gatttcttaa aggtaaatcc
    16621 gtgacgatca aggacagcct tggtcacgct ataatctgca attctcatat attctcttct
    16681 ttttatgatc actttctttt tggcatttga accactagcc taaacttttt tgccaagata
    16741 cgccattcag ttaatggggc tgttatattt tattgtactc tttactcctc atatgtctcc
    16801 atcacttctt ccacttctgc tagggtaatg ccaaataatt cgaggcgctt gagcagttgt
    16861 ttgccgttgg catagccgat gcggagcttt tcgcccaagt attccctgcg tttacggcta
    16921 tctgacgcca ttaaaagccc tagacgaatc aggtctgttt gacgaatgtc aaaataagac
    16981 tcatcatcat attgctgagt gacatgagca agtgcctttt ggaggtcctc aaaattggca
    17041 tgttcaaccc ctaaggaacg gcccttactc ttagaactcg gaaccgcctc gtttcggttt
    17101 aaaaaggcgt gacgagctgt tggcacagct gccataatga gcttacgaat gcgctcacca
    17161 ttataatctg ggtctgtcaa aacaatgaca ccacgcaagt catttaggcg gttaatccgt
    17221 tctaaatctt cttcagtgat agcagagcct ctggtctcat aggtatccac ctcgtaaaaa
    17281 cggcgaaggt tagccgtgtc gtccttgcct tctacaacaa gaacctcttg aatattaatt
    17341 ttttcagtca agcttaagta ccctctttgc attagcagtc gtttcttttg caacttcttc
    17401 cacggtcatt ccccgtagct ctgcgatttt atcaaccaca taacgggtat aagctgtatg
    17461 attttgtttg ccacgttttg gaactggtgt aagataaggg gcatctgttt ccaccaaaat
    17521 cttatccaaa ggcaagtgct gcgctgcctc ttgaatatca agggcctttt tgaaagtgac
    17581 cacacctgaa aaagaaatca tcatgccaag ttcaatgaaa cgttcagcca tctctaatga
    17641 tcctgagtaa gaatgcatga tgcctccacg agggccaaca ccagctgctt tgatgacctc
    17701 ataggtatct tccaaggcat cgcgggtgtg caccacaaaa ggcaaatcat ggtcttttgc
    17761 taattgcatt tggcgcttaa aaacctctat ttgcacctct ttggggtcct ccatccaata
    17821 atagtctaag ccaatttcac ctaaggcaat gactttagga tgtgataatt gagagacgat
    17881 agactcctca actgcttcag aataagatcc tgcttctgtc gggtgccatc caatagtagc
    17941 atacacgtta ggatactggt ctgcgagagc aagtgctcca ctaatagtgg cctggtcaaa
    18001 gccaaccaca ttatggtagg ccacgcccat ttcttgagcc aaagtcaatt cttcagtttc
    18061 gtgtccttga aattctgcta cattcaggtg cgtatgggta tcaaaaatag ttagtttttc
    18121 tactttattt gtcatgatgt tattataaca taaagatctt aaccacatca gaaaacttgg
    18181 tgagaacttc tgccgtttag gttgatcgta aaaccctttg ctagctgaca cttgtctatt
    18241 ttctactttt ttgacaaatg acctttaata gtcggctcct acataagccc tcaaaaatac
    18301 tacaacctac tcaaataaag tgggttgaac aaatctgtat cacaaaaagg gtcgctacct
    18361 tctgttagcg atctatgcta tactagtaga tagttaaact gtaaggagta tgctttgcta
    18421 tgcctaaaaa ccaccgccta attaccaaaa tcgaagcatc attggaacat atgaccagtt
    18481 tagaaaaagg gattgctcat ttttttatta caaccgatct gacacctcaa gagttgaccg
    18541 catccgagat agttaagcga ctacacatct ctcaagccgc cttgactcga tttgcaaaaa
    18601 agtgtggttt tactggttat cgtgcttttg cctttgatta ccttcacagt cttcaagaat
    18661 cacaagagac tttccaatct attcacttag aattgacaaa gcgcgtgctg atggactacg
    18721 atgctcttat taataaaacc tatgagctcg ttaatgaaga aaaattactg aacttagcca
    18781 agctgatcga tgcttctgag cgagtctatt tctttggcaa aggatcttct ggactagtag
    18841 caagagaaat gaagctacgc tttatgcgac ttggtttaat ctgtgatgct tattcagaca
    18901 ctgatggttt tacttgggca aatagcctcg tcaatgaaaa ttgccttgtt tttggttttt
    18961 ctttatctgg caaaacaaac tctgtcatca cagcacttca tcaagctagt cagcgagggg
    19021 ccaaaactgt cctcttaaca acagataatc aaaccgaatt tgacgactcc ttagatatta
    19081 ttcccgtatc atcaacccac caactacatt atgggaatcg tgtgtcacca caatttcctc
    19141 ttcttattat gatggacatt atttatgctt acgttttagc gattgacaaa ccgcacaaag
    19201 aaaaaatctt caaaaacact attattgaca aagaaaataa cggcctttag actacaatag
    19261 caaaaagggc gttgttttct ttttaaaggg tcgtcattca cctcccccag tatacttaaa
    19321 caagaactct tcacgtcata tagactcaca gccactaaga ttagctactc gctaaattat
    19381 tttcgatttt ctcaagagtt ataaatgaac aggatcgctc attcttttgt ttaaaatggt
    19441 agtaagctcc taatatacca gcattatttc catgactagc aaacttcagc tgagttttct
    19501 tggcaagact agaaaccaag tagctatcaa gagctgtctt taatttatca gctaaatagt
    19561 ctttttgcac catgattccg ccaccaagta ccaccacatt tgggttaaca acgtagcaga
    19621 tattagcaat accttgtcct aagtaatcca cctgcttact aatggctgct atacaaatcg
    19681 catcacccgc tttagcctgc tcaaaaatac gacgaccatc ccattggcta atgtcatccc
    19741 cataagctaa ggccacctct tgaacaagag ctgtggtaga tgccagatcc tgaaactgac
    19801 catctgacaa atgaaggtaa ccaacttcgc aagctgaatg actactacca tgaaaaactt
    19861 gggaattaaa caataaacag ccaccgattc ccgtcccaat tgtcaagcaa agagccacag
    19921 gataatcctt ggcactgcca gaaatagctt cggctagacc agcacagtta acatcgtttt
    19981 ccacttcaca cggtaacccg aaagtctctt caatctcttt tttaaactga gttccagcat
    20041 agttaggaat ctgagggccg gcataaaaga tttctccctt atcaggatta accattcctg
    20101 cagaagaaat ggccactcca gccagatcca tttggtcttg ataggtcttt accaagccct
    20161 tgaccttttc caaaatagag ggaccacctt tataagcctc agtggccatt tcctcttttt
    20221 ccaacaggtc tcctgtttca gaaatcaagc catacttgat agcagtacca ccaatatcaa
    20281 ttgctaaata atgcttcatt tgttatcccc tcgcatctta ttaaaaggtt tccttagcct
    20341 gattaatcag agcagcagcc cgttggcaaa taaccctatc ttcctcgacc aactcagcta
    20401 aaggtgatcg aacagaacca atatcaagac cctcattaat acgaagtact tccttaataa
    20461 ccccatacat attgccgtga gcagaaacaa ggacaccgat agtctcatta atagtatatt
    20521 gaagggtctt ggccttttct aaatccttgt caactataag ttgattcagc cttaaaaata
    20581 attctggcat agccccgtag gtgccaccaa tgcctgcagc agcacccatc aagcgccctc
    20641 ctagaaattg ttcatctggg ccattaaaaa caatatgatc gtctccgcca atcgcacaga
    20701 aggtttgaat atcctgcact ggcatggatg aatttttaac gccaatcacg cgcttattag
    20761 ccaacatagt cttataaagg cttggggtca aggcaacccc tgccagttga ggaatattat
    20821 agatgataaa atctgtgtga ggagcagccg aagaaatcgc attccaataa tcagcaactg
    20881 catattctgg taaccggaaa taaataggcg gaatagctgc aatggcatcc acgcccaaac
    20941 gttctgaatg agctgccaat tcaatactat ctttagtgtt gttgcaggca acgtgattaa
    21001 taatcgtcag cttgccctta gcgactgcca tgacattttc taacaccaat tgacggtcaa
    21061 aaacgctctg gtaaatacac tctccagaag agccattaat gtaaagcccc tgaacccctt
    21121 tgtcaatata gtactgggtc aaggcacgca cacgctcagg gctaatagct ccttggtcat
    21181 cgtaacaagc ataaaaggca ggaataatgc cttggtattt cgtaagatct gtcatagtaa
    21241 tgtcctttat tcaatgtcat tattaagtgg gaatcgccta agaagggatt ccatgataat
    21301 caagtagagg taagttaggc tactgatgcc catagtggcc agtagagaca agccacaaaa
    21361 taaggttaac agggttaacg gcaaggtaaa aaccaaaagg ccaatcacca ctagaaaagc
    21421 aaacgtccat gggaataaaa ttcctgccag cagtaaactt cgcttaaaga gtattaaaag
    21481 aggaagctct tttctgacgg cctgaggata agcgtacaaa aagagtataa ctaccaaaaa
    21541 taaaacagca atgcaaagta ctttaaaccc ttgaaaaact aggcctgatt ggtttcgaat
    21601 taagaaaaga tctaagacac aaatactcat tatcaaaagc tcaataagac ctaggcgcat
    21661 cccactttta aaataatgtt taaaatgaga gctataagta gtaacaagct gactaacttg
    21721 accttctcgc caatccaaca aactcgcata caaagccatt ttagcaaccc caatcgttag
    21781 aaggggaagg caggacaaga caaataagag attaaacacg agcaaatcaa acagtgctgc
    21841 gctagccctc atccatttac tatcaagctt gaagagagag tgtaaaagtc cctgtttttt
    21901 cgatgtcaaa tcacaacttc ctttctagaa atacgcttac aaacaaaagc taattcccct
    21961 cttaaacgtt taacacatga cttgcttccc atctgattta tctatgtatg cacctaaatt
    22021 aataagcaac ctataaatta agcttagcga cacgctttat ttagtctaac ctttcacagc
    22081 acccatagta atgccttggg taaatgattt ttggaagaca agaaataccg tcacaatagg
    22141 cactgcagcc atggcagccc ctgccataat caagccataa ttagtagcca tttcagcttg
    22201 catagtggca accccaagtg aaatcgttaa attttcccga gaggttaaca tcaccaactg
    22261 cataaaataa tcattccaag tattgataaa ggtaaaaatt gctagcgctg caaagcctgg
    22321 tttgacaata gggaaagcca cattgaaaaa ggtacgaatt tcgccacatc catcaatctt
    22381 ggcggattcc aataactcgg ttggaatatt ttctgagaac tgtttcataa ggaaaacacc
    22441 aaatggccag cccacaagag gcaaaataac agccgccaaa gtgtcatgga ttcccataaa
    22501 attaacaatc cgcacaagag gcactagaac aacctgtttt ggcaaggcca tagcagcaat
    22561 aaaaatcgaa aacaaaagcc gctgtccata aaaccgcttt ttggccaaag catagccagc
    22621 caaagaggaa gtgccacaaa ccaagaacat ggtcgccacc gaaataaaga cactattcca
    22681 caaccatttt aaggctggat tttgcactac caaggcctta aaattttcaa tagtaggtgc
    22741 ctttggccac cattgcggtg gaatcataat ggtatcggct tgagccttaa atgctcctgt
    22801 cataatccaa taaaatggga aaataaacaa aatggtcaag atgcatagaa taacagtagt
    22861 cagaacatcc gatgcggtta gtttcttttt cgtcatctta atatcctcct aatattccac
    22921 atcattacct aaaattttaa attgagcaaa gctgatgatt gcaatcatga ctgccaagaa
    22981 cacacccatt gtgttggcat agccgtattc agagagctta aaggcttttt catacaaata
    23041 gtacatgagg gtactagttg aataatttgg ccctccagac gtcaagagtt gaatcaaagc
    23101 aaaacattgg aaggaattaa tggttgttat gacagcaata tataaggttg ttggcaggag
    23161 acttggccac ttgatattcc aaaacacctg aaattctgtg gcgccgtcta cacgcgcagc
    23221 ttccatcaaa gaattatcaa tatttcccat agcagcaata tatagaataa taggctgccc
    23281 aacagatgtg gttaataaaa tagcaataat agctaacaaa gcccagtgct tatctcccag
    23341 ccaactaatg ttttgttcaa tcacatgagc atattttaag acataattca aaatccctga
    23401 catcggatca taaatccatt tccaaaccac cgttacggca acacttccgg taacaacagg
    23461 tagaaagaaa actgcccgat aaaacgaacg cgccaccaca tttttgtcat aagtcttagc
    23521 cgccacaaag agggaaaaga aaactacaac aggtaccgaa ccaataacga taatcaacgt
    23581 attgataaga gatttcataa agataggatc ttgaaacatc ctagcataat tggcaaaacc
    23641 aacaaaggta aaatccgtca tggaataatt gaaaaaactc gtcacaaagc ccataatcat
    23701 cggtatcaag acaaaaatca caaagaaaac caagaccgga gctaagaaag cgtatgagat
    23761 gagcgtctct cgcattttta atttattgac gttcacttga aatacctctt ttttctgttc
    23821 aacttttcta aaaagaaaag cttctttcca gtgaccattt gacatgtgca ttgaaaagaa
    23881 gccacttgtt ctgaacactt attgtgtttt tttgattgtc ttgttcgctt tttcagtgaa
    23941 ggctttcaaa gcatcttctg gcttttcatc accattagag acggcttgaa ccattgggaa
    24001 ccaaagagtt ctcatttcag caaacccatc aatcgtgttg tagtatggtg agtagaattt
    24061 tgtccattca gcaatttttt ccattcgttt gtctttgtaa agatccccat aagaagttct
    24121 tacagggaaa gcacctgtac gaacaacatt tttaggtccc cattctttat catcagcgat
    24181 aaactggata aatgtcttag aggcagcgac tttttgttca tctttgttat taaagaccgc
    24241 aaaaccattt acgaggtatt ctagttctgg tttgccatca tctgatggga atgggatttc
    24301 aaggtaatcc actttactag cttctaacaa tttagcttgg atgccgggtt gtgcaggcgc
    24361 ccaaagaatg gtaaaggaag tttggccatt cgcaaagttt tgaatatcag ctgaaccgtc
    24421 gtattgagag ccattcatca ttaaaccatc tttaatccaa ttggaaattt tagtcatggc
    24481 tttaatagag ttagcatcat ctgtggtgta tttggttact ttgtcatcgg taatatgact
    24541 actataaagg ttagcaaaga aggcacgtgg tccttggtca ccaccttgac cattagcaaa
    24601 gaaagaacct gggttatatc ctttgtcctt caatgctttc aagacttttt caaaatcatc
    24661 tgttgtccaa ccctctttca caaggtcaag ga
//
LOCUS       DAUVUY010000016        24667 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.016, whole genome shotgun sequence.
ACCESSION   DAUVUY010000016 DAUVUY010000000
VERSION     DAUVUY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24667
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.016"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1263
                     /locus_tag="VOX58_000626"
     CDS             <1..1263
                     /locus_tag="VOX58_000626"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HES5877877.1"
                     /translation="WGDEGKGKITDFLSADAEVIARYQGGDNAGHTIVIDGKKFKLHL
                     IPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRAHVILPYHIQL
                     DQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERLRINLAEKNRL
                     FEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVLFEGAQGVMLD
                     IDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGPFPTELFDEVG
                     ERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVLSGLDTVKICV
                     AYDLDGKRIDYYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPENARNYVRRVGE
                     LVGVRISTFSVGPGREQTNILESVWASI"
     gene            1590..2633
                     /locus_tag="VOX58_000627"
     CDS             1590..2633
                     /locus_tag="VOX58_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HES5877878.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSAKQITDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIEAAK
                     KAIIEGTITVPEN"
     gene            2806..3345
                     /gene="nusG"
                     /locus_tag="VOX58_000628"
     CDS             2806..3345
                     /gene="nusG"
                     /locus_tag="VOX58_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HES5877879.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            3716..5071
                     /gene="nga"
                     /locus_tag="VOX58_000629"
     CDS             3716..5071
                     /gene="nga"
                     /locus_tag="VOX58_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HES5877880.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFASPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     RYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            5076..5561
                     /locus_tag="VOX58_000630"
     CDS             5076..5561
                     /locus_tag="VOX58_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HES5877881.1"
                     /translation="MYKVPKGLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILENGVYPAFLKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMAHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            5585..7300
                     /gene="slo"
                     /locus_tag="VOX58_000631"
     CDS             5585..7300
                     /gene="slo"
                     /locus_tag="VOX58_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HES5877882.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKPESSELTIEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDMTYPAALQLADKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRT
                     EYVETTSTEYTSGKINLSHRGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            7555..7941
                     /locus_tag="VOX58_000632"
     CDS             7555..7941
                     /locus_tag="VOX58_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877883.1"
                     /translation="MKQQSYQPLRLVYLLVALFAAMLLIARPVMADGEQKDPWELGYN
                     QGLTKGKEDGESAKWTDLTPTVPTDPTIPQPPTVIEETNKGIYKEGYKDGYKEGYNKG
                     FGGSYPLLIPVQFIWGLILHWWGKLS"
     gene            complement(8257..8469)
                     /locus_tag="VOX58_000633"
     CDS             complement(8257..8469)
                     /locus_tag="VOX58_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877884.1"
                     /translation="MKKKLSLFMIATAAVVGLSLATPENQTVSANETTTTQACADGTN
                     CCGYNFRGYCGPITWLYYCIRDGKIY"
     gene            complement(8880..9047)
                     /locus_tag="VOX58_000634"
     CDS             complement(8880..9047)
                     /locus_tag="VOX58_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877885.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGSHQTVSANDASYSNGFYGHN
                     GPDSWLLYTVW"
     gene            9226..9405
                     /locus_tag="VOX58_000635"
     CDS             9226..9405
                     /locus_tag="VOX58_000635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877886.1"
                     /translation="MNKLVAEIHKLVIRLHNQANGEVLVFEDEFEVILSSSSSVKLCQ
                     PAHTAPLLVHVIELV"
     gene            9999..11138
                     /locus_tag="VOX58_000636"
     CDS             9999..11138
                     /locus_tag="VOX58_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HES5877887.1"
                     /translation="MKENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            11350..13851
                     /locus_tag="VOX58_000637"
     CDS             11350..13851
                     /locus_tag="VOX58_000637"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HES5877888.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGNEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDKSKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            14158..15591
                     /locus_tag="VOX58_000638"
     CDS             14158..15591
                     /locus_tag="VOX58_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HES5877889.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            15662..15940
                     /locus_tag="VOX58_000639"
     CDS             15662..15940
                     /locus_tag="VOX58_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HES5877890.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            16063..16548
                     /locus_tag="VOX58_000640"
     CDS             16063..16548
                     /locus_tag="VOX58_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HES5877891.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNHNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKNSPYLEGLDLD
                     S"
     gene            16657..17319
                     /locus_tag="VOX58_000641"
     CDS             16657..17319
                     /locus_tag="VOX58_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HES5877892.1"
                     /translation="MTHLPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     IKEERGGRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIKRI
                     WG"
     gene            17324..18187
                     /locus_tag="VOX58_000642"
     CDS             17324..18187
                     /locus_tag="VOX58_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HES5877893.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLT"
     gene            18189..18893
                     /locus_tag="VOX58_000643"
     CDS             18189..18893
                     /locus_tag="VOX58_000643"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HES5877894.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGSVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            19218..20864
                     /locus_tag="VOX58_000644"
     CDS             19218..20864
                     /locus_tag="VOX58_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HES5877895.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INKALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            21117..22208
                     /gene="ulaG"
                     /locus_tag="VOX58_000645"
     CDS             21117..22208
                     /gene="ulaG"
                     /locus_tag="VOX58_000645"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HES5877896.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            22695..23891
                     /locus_tag="VOX58_000646"
     CDS             22695..23891
                     /locus_tag="VOX58_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HES5877897.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            23907..>24667
                     /locus_tag="VOX58_000647"
     CDS             23907..>24667
                     /locus_tag="VOX58_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HES5877898.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGVFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADVG"
BASE COUNT         7527 a         4574 c         5341 g         7225 t
ORIGIN      
        1 tggggtgacg aaggtaaagg taaaatcact gatttcttgt cagcggatgc agaagtaatt
       61 gcacgctatc aaggtggtga taatgctggt catacgatcg tgattgatgg taagaagttt
      121 aaacttcact tgatcccatc aggaattttc ttcccgcaaa aaatttccgt tattggtaat
      181 ggcgttgtgg tcaaccccaa atctttggtc aaagagttgg cgtacctgca tgatgaagga
      241 gtgacgacag ataaccttcg catttcagac cgagctcatg tcattttacc ttatcatatc
      301 cagctggatc aactgcaaga agatgcaaaa ggtgacaata aaatcggaac aactattaaa
      361 gggattggtc cagcttatat ggataaagct gctcgagttg gtatccgtat cgcagattta
      421 ctggataagg atatctttgc agagcgccta cgcattaatt tagctgagaa aaaccgttta
      481 tttgaaaaaa tgtatgacag tacacctctt gattttgatg ctatctttga ggagtactat
      541 gcttacggtc aagaaatcaa gcaatatgtg acagatactt ctgtaatttt aaatgatgca
      601 cttgatgccg gtaaacgtgt tctttttgaa ggtgcacaag gggttatgct tgatattgac
      661 caaggaactt acccatttgt aacgtcttca aacccagttg ctggtggtgt aaccattggt
      721 tctggtgttg gcccaagtaa aatcaacaaa gtagttggtg tctgtaaagc ctacacaagc
      781 cgtgtcggtg atgggccatt ccctacagaa ctctttgatg aagtgggtga gcgcattcgt
      841 gaagtgggtc atgagtacgg gacaacgacc ggtcgtccac gtcgcgtggg ttggtttgat
      901 tcggttgtca tgcgccacag tcgtcgtgta tcaggtatta ctaacctctc tctgaattca
      961 attgatgttc tttcagggct tgatacggtt aagatttgtg tggcttatga ccttgatggg
     1021 aaacgtattg actattaccc agcaagtctt gaacaactca aacgttgcaa accaatctat
     1081 gaagaattac caggctggca agaggacatc acgggtgttc gtagccttga tgagctgcct
     1141 gaaaatgccc gcaactatgt ccgtcgtgtt ggagaattgg ttggcgttcg catttcaacc
     1201 ttctcagttg gtccaggacg tgaacagacc aatattcttg agtcggtctg ggcatctatc
     1261 taaggtgtct agtatgacaa agagttggat agcgtttcca gctcttttta ccgcttgttg
     1321 gattgtatag ttagtcatgc tattactagt agcttgagtt ttgtcgttga cttatctcct
     1381 taagaggtga tttttgagga gataatggga tttaccagtt gatctcactt tatcgttcgg
     1441 tcattttggt gaaaaagaga tttttcaaca aaaatagtag gataaaactg atttttttga
     1501 tatactaaac ttttcgttct agagcataag tagaacgtta acaatgacac atcactttag
     1561 gattaaacat ttaggaggtt cattttcaca tgaacaaaaa agtaatgtca cttggtcttg
     1621 tttcgactgc cctattcaca ttaggaggct gtaccaataa ctccgctaaa caaataactg
     1681 acaattcatt aaaaatcgct atgattacta atcagacggg tattgatgac aagtcattta
     1741 accagtcagc ctgggaaggc ttgcaagctt ggggaaaaga aaataaactt gaaaaaggaa
     1801 aaggctatga ttatttccaa tcagccaatg aatcagagtt taccacaaac cttgagtcag
     1861 cagtaaccaa tggttataat cttgtttttg ggattggatt tccattacat gacgctgtag
     1921 aaaaagtagc cgcaaacaat cctgacaacc attttgcaat tgtggatgat gtgattaaag
     1981 gtcaaaaaaa tgttgcaagt atcacctttt cagaccatga agcggcatac ctagccggtg
     2041 ttgcagcagc taaaacgaca aaaaccaagc aagttggttt tgtaggtggt atggaaggag
     2101 atgttgtcaa gcgctttgaa aaaggttttg aagctggtgt gaaatcagta gatgatacca
     2161 tcaaagtaag agttgcttat gcaggctctt ttgcagatgc tgccaaaggc aagacgattg
     2221 cagctgctca atacgctgaa ggcgcagatg ttatttatca tgcagcagga ggcacagggg
     2281 cgggtgtctt tagcgaagct aagtctatca acgaaaaacg taaagaagaa gataaggttt
     2341 gggttattgg tgttgaccgt gaccaaagtg aagatggaaa atacaccaca aaagatggca
     2401 agtcagctaa ttttgttttg acctcaagta tcaaggaagt cggaaaagct ttagtaaaag
     2461 tagccgtaaa aacctcagaa ggccaattcc caggtggtca aataaccact tttggtttaa
     2521 aagaaggtgg tgttagtctt acaacggatg ctctgacaca agacactaaa aaagctattg
     2581 aggctgctaa aaaagcgatt atcgaaggaa ccatcacagt tcccgaaaac taaaactgta
     2641 caataccgtc cagagtcgtc actaaccgac tttggacttt ttaataaagg tgtcaagtag
     2701 ctccacagat acgttgacag cagccctgtc ttttattata atgaagatga tagaaaacag
     2761 cgcaaaagcc aaaaaaaggg cttttttata atgaaaggaa acatcatgct agattcattt
     2821 gataagggct ggttcgtcct ccaaacttac tcaggctacg aaaacaaagt aaaagaaaac
     2881 ctcttgcaac gtgcccaaac ctataacatg ttggacaata ttttgcgcgt cgaaattccg
     2941 acacaaacgg ttaacgttga aaaaaatggg caaaccaaag aaatcgaaga aaatcgcttt
     3001 ccagggtatg tcttggttga aatggtcatg acagatgaag cttggtttgt tgtgcgtaac
     3061 acaccaaacg ttacaggttt tgtgggatca cacgggaacc gatcaaaacc aacaccactt
     3121 cttgaagaag aaatccgtgc tatcttgttg tctatgggtc aaaccattga tgtctttgat
     3181 accaatatca aagaaggcga tgtggttcag atcatcgacg gtgcctttat gggacaagaa
     3241 ggtcgtgtgg tagaaatcga aaacaacaaa gtgaagctga tgctcaacat gtttggctct
     3301 gaaacagtag cagaagttga actttatcaa attgcagaac tataggctct caaaggtctg
     3361 atggtataaa tattaccatc agaccttttt tagttggaag tgaagcgtgt gtacaaacaa
     3421 aggtatgact atcgttaaca tcattgtaat cagagataat taacagatct attactgata
     3481 acggtgctac tatcaaatat ttaactagta cttttaatct ggtttaaata gaacagatgt
     3541 gaaggttctg tgataataag atggaaatat ccccttgaaa cttgtaacag ttggctactt
     3601 gtttttttgt gttcattttt ttgatagaaa agtctaaaac gatgttaata aatcgcattg
     3661 acaaaaacta tttgctaatg tatagtttac ttaaaaataa tataaggtgg tttacatgag
     3721 aaacaaaaaa gtaacattag ctcatattgt cgcaaagaca agtgttgcta ttgctttggc
     3781 tggagcaatg ggtagcagtt tattagctaa tagcacaacg tacgctgtta gtggcaaaga
     3841 aaataaaaaa agcgatgtca aatatgaaac gaccaaagtt atggaagcta acgcaacttc
     3901 ctctaaagaa gacaatcatg tcatgcacac attagacggc tcaatgagta ctgtctggga
     3961 ggaaaattca cctggtggtg gtgttggtga ggtactttcc tacaagtttg cttccccgat
     4021 gcgtattggg agaattttaa ttgttaatgg agatacatct agcaaggaga attactacaa
     4081 gaaaaataga attgcaaagg ctgatgttaa atactataac gggaataaat tggtcctctt
     4141 tcaaaaaatt gaattaggcg acacctacac taaaaaaccg catcacattg agattgataa
     4201 aaaattagat gttgatcgta ttgatattga ggtaacagag gtccatcagg gacaaaacaa
     4261 ggatatttta gccttgtcag aggtcacttt tggcaatatg gaacgcgatc tttttgaaaa
     4321 aaagtttaaa gaaattaaag ataaatgggt aacagataaa caagcagatg agtttattga
     4381 aactgccgac aaatatgctg ataaagctgt tcagatgtct gctgttgcgt cacgtgctga
     4441 gtattaccgg atgtacgtta gccgcaaata ccactacaaa aaagagtttg ttgaaaaact
     4501 aaaacaagtc tacaaagaaa gcggagcttc tcacgttaca agtaaaaaag atttgatgtt
     4561 agcttttgat gatgctaaaa gaaagtcaac gattggtcga caagaaaacg gtctttttgt
     4621 gacaagtttt gctgaagata tggcattgct ctttactgat caaggtaagt taaaatcagc
     4681 tgaccaaatt gaaaatataa aaggtgtcga tagcggaaga tatagcgatg gggtttatca
     4741 gtatgagtac gattctgaac taacaaaaaa cattgataag ctaggctata ttcgaacagc
     4801 tagcggagat actcctggag caaattcgct caacattcct ggttgccaaa cgtggtcagg
     4861 aaaacacatt gaaaattcag aaagtgaatt aattttccca tcgattagtg ttaaagatct
     4921 aaaatctaaa gctgtcttag cagagattga tgccaaaggc tattttgaaa ttattgatcc
     4981 taccatcatt gctccaaatg gtgaccataa aaaagtaact ggtcgcttca aaattaagaa
     5041 aatgcaagat agaatgcaag ataggaagta acaatatgta taaggtgcca aagggtttag
     5101 aacattacca aaaaatgttt caaaaagaag taactgttaa tgatttaaaa aaatacttga
     5161 taggtagtga caaagagtat cgtatcacta gaagagactc ttatatggga gatatttctg
     5221 atccagaagt tattttagag aatggggttt accctgcttt cctaaaaggc tatacccaat
     5281 tgaaagctaa catcgaagaa gcattattag aaatgtcaaa tagcggtcaa gcattagaca
     5341 tttaccaagc ggttcaaacc ctaaacgctg aaaacatgtt attaaattat tacgaaagct
     5401 tgccatttta tttaaaccgt caaagcatac tagctaatat aaccaaagcg ttaaaagatg
     5461 cgcatattag agaggctatg gcacattaca aattaggaga atttgctcac tatcaagata
     5521 ctatgcttga tatggtcgaa agaacaatag aaacatttta gaatgataaa aaggtatgaa
     5581 ggacatgtct aataaaaaaa catttaaaaa atacagtcgc gtcgctgggc tactaacggc
     5641 agctcttatc attggtaatc ttgttactgc taatgctgaa tcgaacaaac aaaacactgc
     5701 tagtacagaa accacaacga caaatgagca accaaaacca gaaagtagtg agctaactat
     5761 cgaaaaagca ggtcagaaaa tggatgatat gcttaactct aacgatatga ttaagcttgc
     5821 tcccaaagaa atgccactag aatctgcaga aaaagaagaa aaaaagtcag aagacaaaaa
     5881 aaagagcgaa gaagatcaca ctgaagaaat caatgacaag atttattcac taaattataa
     5941 tgagcttgaa gtacttgcta aaaatggtga aaccattgaa aattttgttc ctaaagaagg
     6001 cgttaagaaa gctgataaat ttattgtcat tgaaagaaag aaaaaaaata tcaacactac
     6061 accagtcgat atttccatta ttgactctgt cactgatatg acctatccag cagcccttca
     6121 gctggctgat aaaggtttta ccgaaaacaa accagacgcg gtggtcacca agcgaaaccc
     6181 acaaaaaatc catattgatt taccaggtat gggagacaaa gcaacggttg aggtcaatga
     6241 ccctacctat gccaatgttt caacagctat tgataatctt gttaaccaat ggcatgataa
     6301 ttattctggt ggtaatacgc ttcctgccag aacacaatat actgaatcaa tggtatattc
     6361 taagtcacag attgaagcag ctctaaatgt taatagcaaa atcttagatg gtactttagg
     6421 cattgatttc aagtcgattt caaaaggtga aaagaaggtg atgattgcag catacaagca
     6481 aatcttttac accgtatcag caaaccttcc taataatcct gcggatgtgt ttgataaatc
     6541 agtgaccttt aaagagttgc aacgaaaagg tgtcagcaat gaagctccgc cactctttgt
     6601 gagtaacgta gcctatggtc gaactgtttt tgtcaaacta gaaacaagtt ctaaaagtaa
     6661 tgatgttgaa gcggccttta gtgcagctct aaaaggaaca gatgttaaaa cgaatggaaa
     6721 atactctgat atcttagaaa atagttcatt tacagctgtc gttttaggag gagatgctgc
     6781 agagcacaat aaggtggtca caaaagactt tgatgttatt agaaacgtta tcaaagacaa
     6841 tgctaccttc agtagaaaaa acccagctta tcctatttca tacaccagtg ttttccttaa
     6901 aaataataaa attgcgggtg tcaataacag aactgaatac gttgaaacaa catctaccga
     6961 gtacactagt ggaaaaatta acctgtctca tcgaggtgcg tatgttgctc aatatgaaat
     7021 cctttgggat gaaatcaatt atgatgacaa aggaaaagaa gtgattacaa aacgacgttg
     7081 ggacaacaac tggtatagta agacatcacc atttagcaca gttatcccac taggagctaa
     7141 ttcacgaaat atccgtatca tggccagaga gtgcaccggc ttagcttggg aatggtggcg
     7201 aaaagtgatc gacgaaagag atgtgaaact gtctaaagaa atcaatgtca acatctcagg
     7261 atcaaccctg agcccatatg gttcgattac ttataagtag gactggttca agaggttcgt
     7321 caagcacctt gatgctggtt atctcttgag accttgtgcc tactcctctc acaaatacag
     7381 aagaaagctt gtctatgaga taccttcata gacaagcttt tttgatatac taatcaaaaa
     7441 gttattcttt tctcacatag tgaccttgaa gctaagctgc ttctaatcta taatactaac
     7501 aagatacttt ttaacaagca aagcataata taaaagggag agataactat tatcatgaaa
     7561 caacaatctt accagcctct acgcctcgtc tacctcttgg tggctttatt tgctgctatg
     7621 ttgcttatag caagacctgt tatggcggat ggggaacaaa aagatccgtg ggaactaggc
     7681 tataaccaag gtttaacaaa ggggaaagaa gatggtgaat cggcaaagtg gactgatttg
     7741 accccaactg tcccaactga tccaactatt ccacaaccac ctactgttat tgaagagact
     7801 aataaaggta tttataaaga aggatataaa gatgggtaca aagagggtta taataaagga
     7861 tttggaggaa gctacccttt gttaattcct gtccagttta tatggggact cattttacat
     7921 tggtggggga agttgagctg acatttgata atcaactcta agctgattcc atagctagtg
     7981 gctatagtta ggaaaaaacg aatttaaaac agttccttgc taccacacct taaaccgcac
     8041 tttaatcatt aggttttagt gatatgtgcc caggattctg gggacatagc ctatgtctaa
     8101 ttgttggggt gcggtttaat tgtctttggg tttgaagttg tggcaacacc ctaactttag
     8161 gacagcaaaa aagcccagga ctgcctaaat aggcacctaa gcttttgagt acttcagact
     8221 tactaatgag atatttctct taatttagat gtaatattag taaatttttc cgtctcggat
     8281 acaatagtaa agccaagtga tagggccgca ataccctctg aaattgtaac cacagcaatt
     8341 tgttccatct gcacatgctt gagtagttgt agtctcattt gctgaaacag tttggttttc
     8401 tggtgttgca aggctaaggc ctacaacagc agcagttgcg atcataaata aagataattt
     8461 ctttttcatt tgtaactcct taaattatag tatacctata ttatactcat attctattaa
     8521 ttattcaata tcatatttta atctttttgt gatgtgtttg accgaaaaag gcaaagaaag
     8581 ccaagactaa agtatttttt atcttatata aatcacatta attactaata atattaatct
     8641 ggttatcatt ctatttgagg tgttacagct tttttggtgc tggttagcta gcaactgtta
     8701 cgacttgtgt tttgggcttc tctcctttta taggcagtgt gccttttctg ttttaccttg
     8761 aggtgtcatt ggcaaaaacg ttttagccaa tcaaaaagcc taaggctgct tatgcagcct
     8821 taggcttttc ataaccgtag tcttacgaag aagatagctt ctatattacg gtatgaggtt
     8881 taccatactg tgtaaagtaa ccagctgtca ggaccattgt gtccgtagaa accgttacta
     8941 tagctagcat cgtttgctga aacagtttgg tggcttccta gtgttgcaag gctaaggcct
     9001 gcaacagcag cagttgcgat cataaataaa gataatttct ttttcataga taactccttt
     9061 aatttgtatt gtgattatat tatactaact ttttgttcat aaatcaagta ttattttaat
     9121 gtttttatta ttatttatct gaataagaaa tgtggcgacc taggattaga aaaccgccac
     9181 gagatggtta tttaaattcc atggatatga ttatagagat cttaggtgaa caagcttgtt
     9241 gcagaaatcc acaaacttgt tattaggctc cataaccaag ctaacgggga agtattggtt
     9301 tttgaagatg aatttgaagt tatactgtct tcttcttctt ctgttaagct atgccagcca
     9361 gcgcatacag ctccactgtt agtacacgtc attgagttgg tctgaggttc ttttgcctgc
     9421 tcagtcgcat gcacggtctg gtgatgaggg gttgctaaag acggccctag gacggttacg
     9481 gtcgcgaggg caactaaaaa taatttgttt ttcactatat ttctctccta aaaataaata
     9541 gtactttagc agtgtactat aagacgcatg gtgaaaacaa gttctgtttc tcattaaatg
     9601 aaataaattg tgacctttta atgaaatttt gatggcaata ttgccgtatt gagtaggtga
     9661 cgatgcaaag taggactgtc agtgtgataa gctaagcttt tgagacgatg ggaccaaagg
     9721 tagctatact ttaacttgct ggagaggtgt tggcagcttg gtcaaagatg ataaaagggc
     9781 ttgcaaactt agctaaaggt tgtataataa acgtaatcga tctaaaaatg atattgttgg
     9841 aaaaattcat tatccgttta aaaggtgccc actacttttc ttataggtta gcagatcacg
     9901 cttttttgaa gagctttttg gcttgtgcaa ccattggggt gggaccattg tctaaaagag
     9961 agttcattgg agtcaacgct attgcggagg tgcacgaaat gaaagaaaac actactttat
    10021 tacatggcta tacggttatt gacgagttta caggagcagc ttcagttccc atctatcaga
    10081 cttcgacgtt ccacaattca gaactgtatt gtcctagtca aaaacatttg tacacacgct
    10141 tttcaaaccc aacgactgaa gctttagaag acgggttagc ctgtttagaa aaagcgactt
    10201 atgcggtggc ttatgcatct ggtatggctg ctatttcaac tgttttgatg ctcttaaaag
    10261 ctggagatca tgtgattttc ccattggagg tctatggagg gacttgtcaa tttgcgacag
    10321 ctattttacc caattatcag attgaaacaa gctttgtaga tatggctgat ttagcaacag
    10381 taaaagcaag tatcaggcct aatacgcgta tgatttatct agagacaccg tcaaatccgc
    10441 tcttgaaaat ttgtgatatt tcagagcttg tgcaactcgc caaagcttat ggtgtgctaa
    10501 ctgtggctga taataccttt atgacaagtt tgtatcaaga acccttagct atgggtgtgg
    10561 atattgtggt agaaagtgtg accaaattta tcaatggtca tagtgatgtg gtagctggtt
    10621 tagctgctac caataatgag gctatctaca accagctcaa gctttttcaa aaaaactttg
    10681 gagcaattgt tggggtcgaa gacgcttggc tgattcttcg tggcatgaaa actatgggga
    10741 tccgtatgga acaagcggta aaaaatgctc agcagctcgc taactattta gcgaagcacc
    10801 ccaaagttct taaggtacat taccctggct tagacagtca tcctaatcac gatacccacc
    10861 tgcaacaagc taagaatggt ggtgctgttt tatcctttga gttggcttct aaggaggagc
    10921 tgatgacctt tacccatcgt attcagttgc cgattttagc cgttagtcta ggtggagtag
    10981 aatccatttt gtcacatcca gcaacgatgt ctcatgcttg tttatcatca caagcgcggt
    11041 tggaacaagg ggttgttgat ggtttgctgc gcttgtcttg tggtgttgaa aatatcgaag
    11101 atttattggc cgattttgag caagcacttg ctttttaagt ctatttgtcc agattggctc
    11161 tgtttacaga gcaagtctgg ctttttttat tagctttttg tgaggatggt tgatgctaaa
    11221 gcagggttta aaaatagcta atcgtctgaa gtctcaaaag ccaaggtgct agtcatgtct
    11281 tgaccattcc taaaagcttc atctcatgct ataataagaa gactagattt tttacatggg
    11341 agatttcaca tgacatttta cgatcacacc gctattgaac ccaaatggca agccttttgg
    11401 gcagataatc atacttttaa aacaggaaca gacgcatcaa aacctaagtt ttatgcccta
    11461 gatatgttcc catacccatc aggagctggt ctccacgttg gtcacccaga aggctataca
    11521 gcaacggata ttctcagccg tttcaaacgt gcccaaggtc acaatgtcct ccacccaatg
    11581 ggttgggatg cctttggact tcctgctgag cagtacgcta tggatactgg taatgaccca
    11641 gccgagttta cagctgaaaa catcgctaac ttcaaacgcc agattaatgc tcttggtttc
    11701 tcatacgact gggatcgtga agttaacaca actgacccta actactacaa gtggacgcag
    11761 tggatcttta ccaagcttta tgaaaaaggc ctagcctacg aagctgaagt tcccgttaac
    11821 tgggttgaag aattgggaac agctatcgcc aacgaagagg tgctccctga tgggacatca
    11881 gaacgtggcg gctacccagt tgtcagaaaa cccatgcgcc aatggatgct caaaatcact
    11941 gcctatgcag agcgcttgct agaagacttg gaagaagttg actggccaga atcaatcaag
    12001 gacatgcaac gcaactggat tggtaaatca actggtgcta atgtaacctt caaggttaag
    12061 gatacagata aggacttcac tgtctttaca acacgtcctg acaccctttt tggtgcaact
    12121 tacgcggttc ttgctcctga gcatgccttg gttgatgcta ttacaacagc tgaccaggca
    12181 gaagccgtgg ctgactacaa acgtcaagca agtctcaaat cagaccttgc tcgtacagac
    12241 cttgccaaag aaaaaactgg tgtctggacg ggtagctatg cgattaaccc agttaatggt
    12301 aacgaaatgc cagtttggat tgcggactat gtccttgcca gctacggtac tggtgctatc
    12361 atggctgttc cagcccacga tgaacgtgac tgggaattcg caaaacaatt taagctagat
    12421 atcattccag ttcttgaagg tggtaatgtt gaagaggctg cctttacaga agacggtctt
    12481 cacatcaact cagacttcct agatggtctg gacaaagcgt cagctattgc caaaatggtc
    12541 gaatggcttg aagcagaagg cgtcggtaat gagaaagtca cttaccgtct tcgtgactgg
    12601 ctctttagcc gtcaacgtta ctggggtgag ccaatcccaa tcatccactg ggaagatggg
    12661 acctcaacag cggttcctga gagtgagttg ccactggttc ttccagtaac gaaggatatc
    12721 cgtccttcag gtactggtga gagcccactt gctaacgtga cagactggtt ggaagtgact
    12781 cgtgaagatg gtgttaaagg tcgtcgtgag actaacacca tgccacaatg ggcaggttca
    12841 agctggtact atctccgtta catcgatcca cacaacaccg aaaaactggc tgatgaggaa
    12901 ctcctcaaac aatggttgcc agttgatatc tatgtcggtg gtgcagagca tgccgtgctt
    12961 caccttctct acgctcgctt ctggcacaag gttctttacg atctcggtgt cgtaccaacc
    13021 aaagagccat tccaaaaact ctttaaccaa gggatgattt tgggaacaag ctaccgcgac
    13081 agccgtggag cgcttgtagc gactgacaag gttgaaaaac gtgatggcag cttcttccat
    13141 gtggaaactg gtgaagagct tgaacaagca ccagctaaga tgtctaaatc acttaagaac
    13201 gttgtcaacc cagatgatgt tgttgagcag tacggtgctg acactcttcg tgtttacgag
    13261 atgttcatgg gaccacttga cgcttccatc gcttggtctg aagaaggtct tgaaggctca
    13321 cgtaagttct tggaccgtgt ttaccgtttg atcacaacta aagagatcac tgaggaaaat
    13381 agcggagccc ttgacaaggt ttacaacgaa actgttaagg ctgttactga gcaagttgac
    13441 caaatgaaat tcaacacagc tatcgctcaa ctcatggtat ttgtcaatgc agccaacaag
    13501 gaagacaaac tctttagcga ctatgccaaa ggcttcgttc aattgattgc accattcgca
    13561 ccgcacttag gtgaggaact ctggcaagtc ttgacagcct caggtgagtc aatctcttac
    13621 gtgccatggc caagttatga taaaagcaaa ttggttgaaa acgatgtgga aatcgttgtc
    13681 caaatcaaag gtaaggtcaa agctaaactt gtcgttgcca aagacctttc acgcgaagaa
    13741 ttgcaagaag ttgctcttgc taacgaaaaa gtccaagccg aaatcgcagg caaagacatc
    13801 atcaaagtca tcgcagtacc aaacaaactc gttaatattg ttatcaaata gtttgaggtg
    13861 acacagccct tcagtggtta tttgcgtcac gtcacagtta atgccaaaga gctgatctat
    13921 tcaaaaagca actcatctag aaaaaccaca agaaccttcg ttcttgtggt ttttaatcgt
    13981 tgtttagggt gattttctct ttattattaa gtgtagttaa attttaaaat aagttaactt
    14041 aagtctaata ttgaacactt ctttgtgtga tagttggtct tttttaaaac gctttcatta
    14101 cctataatga acatataaac actttatctt tattaacttt gaaaagaggt agttcttatg
    14161 gaaatgctcc tagctcccct aaactggttc tcgcaaaata ttttgcaaaa cccagcgttc
    14221 tttgtaggtc ttttggtttt aatcgggtat ttactcctta aaaaacccat ttatgaggtc
    14281 tttgctggtt ttgtaaaggc taccgttggt tacttgattt tgaacgttgg agcaggcggt
    14341 cttgtaacga cttttcgtcc gatcttggtt gctcttgcca agaaatttga attgaaagcc
    14401 gccgttattg atccttattt tggtttggct gctgctaata ctaagttaga agagatgggc
    14461 ttcatcagtg ttgctacgac agctctcttg attggatttg gggtcaacat tcttttggtt
    14521 gcacttcgca aggtcactaa agttcgtacc cttttcatca caggtcacat catggtccaa
    14581 caggcagcga cgatttcggt ctttgtcctt cttttaatcc ctcaattcca aaatgccttt
    14641 ggtgcgtggg cagttggtat catctgtggt ctttactggg cagtcagctc aaatatgacg
    14701 gttgaagcaa ctcaacgcct tacaggtggc ggtgggtttg cgattggtca ccaacaacaa
    14761 tttgcgattt ggtttgtgga taaggttgca ccgttctttg gtaaaaaaga agaaaacctt
    14821 gataacctaa aattaccaac cttcttaaac attttccatg atactgttgt tgcgtcagca
    14881 actttgatgt tggtcttttt cggagctatc ttggctgtcc ttggtccaga cattatgtct
    14941 gatgtggact taatcggacc tggagcattc aacccagcta agcaagcttt cttcatgtat
    15001 attttgcaaa cctctctcac cttctcagtt tatctcttta tcttgatgca gggtgttcgc
    15061 atgtttgttt ctgaattgac caatgccttt caagggatct caagcaaatt gcttccaggg
    15121 tcattcccag cggttgacgt tgcagcttcc tatggctttg gttcttcaaa tgcggtgctt
    15181 tcaggatttg cctttggttt gattggtcaa ttaatcacca ttgctcttct tgttatcttt
    15241 aaaaatccaa tcttaattat cacaggattc gttcccgtat tctttgataa tgcggcaatt
    15301 gcggtttatg cagataaacg tggtggctgg aaagcagcgg tagctctttc ctttatctca
    15361 ggtattattc aggtggcact tggtgctgtt gctgtaggtc ttttaggact tacaggtggt
    15421 tatcacggta atatcgactt ggttcttcca tggttaccat ttgggtacct cttcaaattc
    15481 cttggtattg cagggtatgt gcttgtttgc atcttcttac ttgccattcc acaattgcaa
    15541 ttcgcaaaag ctaaggacaa agaagcttac taccgtgggg aagctcaata acactgcatc
    15601 ttaagaaata gctctgctat tttaacaaaa caataataaa acattttcgg aggaaaaaac
    15661 aatggttaaa gtcttaacag catgtggcaa tggtatgggg tcatctatgg tgatcaaaat
    15721 gaaagttgaa aatgcgcttc gtcaacttgg tgtgacagat attcagtcag cttcttgctc
    15781 agttggtgaa gctaaaggtc tagcttctgg ctatgatatt gtagtggcat caaaccactt
    15841 gattcatgag cttgatggtc gtaccaaagg ccacttggtt ggtcttgata acctgatgga
    15901 tgataatgaa attaaaacca aactacaaga agtgctataa gctaattctg aatcaaaacg
    15961 aaggagttgt gacgaaggtt ttcagctctt tctctttagt aggataaaac tagtaaaaat
    16021 ggtgtgtgtt gcaaagcaag acactagaaa gagatagttt ttatgaattt aaaacaagcc
    16081 tttattgaca acaactccat ccgattgggg ctaagtgctg atacttggca agaggctgtt
    16141 caattagctg ttcaaccttt gattgatagc aaagcagtga cgtcagctta ctacgacgcc
    16201 atcattgcat caacagaaaa atacggtcct tattatgtgt tgatgccagg tatggctatg
    16261 ccccatgcag aagcaggttt aggcgttaat cacaatgcat ttgctttgat tacactaaca
    16321 aaaccagtta ccttctcaga tggtaaagag gtttctgttc ttttgacgtt ggccgctaca
    16381 gacccttcta ttcacacaac agtggctatc ccacaaattg tggccttgtt tgaactagat
    16441 aacgccattg aacgcttggt ggcctgccaa agtcctaaag aggttctaga aatggtagaa
    16501 gaatcaaaaa atagcccgta tttagaaggt ttggatttag actcttaatc gatagcttac
    16561 tgacttaaac ggataaaacg actcacaatc attattgaaa ggataagaac tcctcatcag
    16621 ctttttcata gccgctgaga gttctaggaa aaacacatga cacatcttcc aaatttacaa
    16681 gttgctttag accattctga cttacagggg gctgttaaag cagccgttgc tgtgggtcac
    16741 gaagtagatg tgattgaagc tggtaccgtt tgtttgcttc aagtgggaag cgaactagtt
    16801 gaggtattac gcagcctctt cccagagaaa attatcgtag cagataccaa atgtgctgac
    16861 gctggcggta cggtcgctaa aaacaatgca aaacgtggtg ctgactggat gacctgcatt
    16921 tgctgtgcga cgattccaac catggaagca gccctcaaag ccataaaaga agagcgcggt
    16981 ggccgtgggg aaattcaaat cgaactttat ggtgattgga cttatgagca agctcaatta
    17041 tggcttgacg ctgggatttc ccaagcgatt taccaccaat ctcgtgatgc ccttcttgct
    17101 ggcgaaactt ggggtgaaaa agaccttaac aaggtgaaaa cgttgattga catgggcttt
    17161 cgtgtctcag taactggtgg gcttgatgta gacactttga aactttttga aggggttgat
    17221 gtctttacct ttatcgcagg gcgaggcatt actgaagcag aagatcctgc tgcagcggcg
    17281 cgtgccttca aagatgaaat caaacgtatc tgggggtaac tttatggcgc gtcctattgg
    17341 tatttatgaa aaagcaacgc caaaacagtt tacctggcgt gaacgcttgc aatttgcaaa
    17401 agacttaggt tttgattttg tggagatgtc tgttgatgaa tcagatgctc gcttagcgcg
    17461 cttagagtgg actaaggaag aacggcttga tttagtgaaa gccatttatg aaacaggcat
    17521 tcgcatcccg accatttgtt tttctggtca tagacgttac ccactaggct caaatgatcc
    17581 tgctattgaa gccaagtccc ttaaactgat gaaacaatgt attgaattgg cacaggactt
    17641 aggcgttcgt accattcaat tagctggtta tgacgtttac tatgaagaaa aatcgcctga
    17701 aacccgtgct cgttttatca aaaacttgag acaatcttgt gactgggccg aagaagccca
    17761 agtgatgctt tctattgaga ttatggatga cccttttatc aactctattg agaaatactt
    17821 ggcagttgaa aaagagattg actcgcctta tctctttgtt tatcctgatg caggcaatgt
    17881 ttctgcttgg cataatgatt tgtggagtga attttacaat gggcacaaat ccattgcagc
    17941 cctccacttg aaagatactt atgctgtcac ggaaacctca aaagggcagt ttcgtgatgt
    18001 gccatttggt caaggttgtg tggactggca agagctgttt gcggtcctca agaaaaccaa
    18061 ctacaatggg ccttttttga ttgaaatgtg gtcagaaaat tgtgacacgg tggaggaaac
    18121 aaaagctgct atcaaagaag ctcaggactt cctttaccca cttattgaga aagcggggtt
    18181 gacataatat ggcgaaaaac ttgcaagaaa tgcgtgaacg tgtctgtgcg gctaacaagt
    18241 ctctaccaca acacggtctt gtcaaattta cgtggggcaa tgtctctgaa gtttgtcgtg
    18301 aattaggacg tattgtcatc aagccttctg gggtggacta cgacctcttg acaccagaaa
    18361 atatggtggt gacagacctt gatgggaatg tggtcgaagg tgatttgaac ccttcttcag
    18421 atctgccaac ccatgtggaa ctttataaag cttggccaga agtgggcggt attgtccaca
    18481 ctcactccac agaagctgtc ggttgggcac aggcaggtcg tgatattccg ttttatggga
    18541 ctacccatgc tgactatttt tatggcccgg ttccttgtgc tcgttctctc acaaaagcag
    18601 aagttgatgg ggcttacgag caagaaactg gcagcgtgat tttagaagag tttagcaagc
    18661 gtggtcttga cccgatggcg gttccaggaa tcgtggtgcg taatcatggc ccatttacgt
    18721 gggggaaaac cccagagcaa gccgtttacc actcggttgt cttagaagaa gtggctaaga
    18781 tgaatcgctt gacagaacaa atcaatccac gcgtagagcc agcgccaaga tacattatgg
    18841 ataagcatta tttgcgtaaa catggcccaa atgcttatta cggtcaaaaa tgaggtcacg
    18901 atatggctta ttagcacagg ttctcactag aagccaaaaa gttcaaggtt ttgttaatca
    18961 ttgtttgggc aagtcctttt gacttgtcct tttcgatgaa ctgtttaaac gccaatgagc
    19021 acaaactgga tgaaccgtat gttgtggagt tcttagagac cagatataag gtgttggtat
    19081 tggatcagtt gaggtgatta gctcgaatca tattaagcga atcttattat ggtgagtttt
    19141 tcaaaacttg gctgcgcagt cactgaaatc atgataaaat aagcaaggat ggattttcag
    19201 agaggaggtt tgtaaacatg atgattttag ataaaaagag ttatgacttg ctgtcttatt
    19261 tacttaaact agaaacacct gagacagtta tggccatttc tcatgcttta aatcagtcgc
    19321 gtcgtaaagt ttattaccag cttgacaaaa ttaataaggc tttgccaaag ggagtagatc
    19381 agattatctc ttatcctaga cttggcattc ttttaacggc ggatcaaaaa gctgcttgtc
    19441 gcttattatt agaagaagtg actgattata attatgtcat gaaaagtgat gagcgcagac
    19501 gcctatcctc gatttatatt gcggtttcca ctgagcgcgt gaccattgat aaactgatgc
    19561 agatcaatga tgtgtctcgt aacacgattt taaatgacct gactgagtta agggaagaac
    19621 tggaagacaa acaatacaag attcagcttc atgcgacaaa ggctagggga tattattttg
    19681 gttgtcatcc aatggctctt attcaatacc tgtacaaatt gttagtggac gtttaccaag
    19741 gtggcaatac tagctttatt gatatattca accgtaaact ctcagagata caaggcttat
    19801 cggtttattt ttctaaagat attttgactt attttcatga gtatctcttt ttatctcaag
    19861 ctagcttagg aaagacaatt aatacccaag atagccaatt catgttgcag attttacctt
    19921 tcatgctttt aagttatcgt aatatgcgtc ttgattcaga aacaaagtct gctcttaaac
    19981 aagaatttca tttgatttgg aaacgtaaag aatatcacat tgctcaggat ttagctagag
    20041 agctttacca taattttaag cttcatttgg atgatatcga agttagtatg gtagcgatgt
    20101 tgatgttgtc ttttcgaaaa gatcaagatc atcacgtgga aagtcaagat tatgacgaca
    20161 tgcgagcaac cattagtcac tttattgacc agttggagag ccgttatcag ttacatttca
    20221 cgcataaaca ggatttgctg aagcagttga cgacccactg taaggcgctc gtttaccgta
    20281 aagcttatgg gattttttta gttaatccct taacagacca tattaaagaa aaatacgaag
    20341 aattgtttgc tatgactcaa tcttgtgcca ctattttgga acaagattgg acgattagct
    20401 tgacagacga tgatattgcc tacttgacta tccatcttgg gggagagctg cgtcacaata
    20461 atacagagca agaaaagacc aaactcgtta ttgtttcaga tgatgggata ggcattcaaa
    20521 agttattgtt caaacagtgt caaaggtatc tagcaaatgg tcagattgaa gctgttttta
    20581 cgacagaaca gtatcagagt gtttacgacc ttttagcagt cgatatgatt gttgctacta
    20641 cagatacgct aaagactaaa atcccgatgc ttattgttaa ccctatttta agtgacgatg
    20701 atatcatcaa attgatacgc ttttcaaagc aaggacgctt gagtgagcac tcccgctttt
    20761 ctacagagct cacaaaagct attgaagctg ttgtaaaaga tgaatcagat cgttatgcct
    20821 tggtttctaa aattgaaaaa ctcattcatc gcgagctgct atgagtaagt gttaagtcgt
    20881 caaagtacct cagttttcta tgacatagaa aaatataact gaggttttta atatagcttt
    20941 gaatacagta ttgatgcgag atacagtgtg caaaatgaga cagtattaga ctgagctaat
    21001 gaaaaagtca gtccactttt gaacacagat ctgtgtgaca cttggtcttt ttatcattga
    21061 caagaaagtc tatacttaaa gtatagaaac gaagaacaaa ttcggaggaa accctaatga
    21121 ctaaagtaca agatattaca cgtgaatcat ggatccttaa cacgtttcca gaatggggaa
    21181 catggttaaa tgaagaaatc gagcaagaag tcgtgccagc tgacaacttt gcgatgtggt
    21241 ggttaggaaa ctgtggtatc tggattaaga caccaggcgg tgctaatgtt gtcatggact
    21301 tatggtcaaa tcgtgggaaa gcgaccaagc aagttaagga tatggttcgt ggacatcaaa
    21361 tggctaatat ggcaggagct cgtaaattac agcccaatct tagggcccaa ccaatggtga
    21421 ttgatccatt tatgattaat gagttggatt attatttggt ttctcattat catagtgacc
    21481 acatcgatat caatactgcg gcagccatta tcaataaccc aaaacttaat catgttaaat
    21541 ttgtaggacc ttatgaatgt ggtgaagttt ggaaaaattg gggtgtgcca aaagatcgta
    21601 ttatgatctt aaagccaggt gatagctttg aattcaaaga tattaagatc accgctgtag
    21661 agtcgtttga ccgcacctgt ctggtaactc tcccaataca aggagctgat gctcaagatg
    21721 gtgatttagc aggtttagct atcacagatg atgacatggc tcgcaaagct gttaactata
    21781 tttttgaaac accaggtggt accatctatc atggtgcgga ttctcatttt tcgaactact
    21841 ttgctaaaca tggacgtgat tatgatattg acgttgtgtt aaataactat ggggaaaacc
    21901 caattgggat tcaagacaag atgacttctg ttgaccttct tcgtatggca gaaaatctcc
    21961 gtgctaaagt cgttatccca gttcattatg acatttggtc aaactttatg gcatcaacag
    22021 atgaaatcct tgaactgtgg aaaatgcgca aagaacgtct ccaatacgat ttccatccct
    22081 ttatctggga ggttggtggc aagtacacct atccacaaga ccaaaatcgt atcgaatacc
    22141 atcatcctcg tggatttgat gactgcttct tagaagattc taatatccaa tttaaagctt
    22201 tactataagc aacaccctac tcacctaaga tgatagtgcc tgattaagct aactcgcttc
    22261 ataggacact gtcttaggtg agtttttttg gagagatgag caaatgttta agagtcaccg
    22321 tggtcgttat ttacgatggc cgcaaaagag atattatagg taaaagaagg atttctaatg
    22381 taacaatagc ctaatcagca aagaaatagt catcagttgt gataaaaaat tgtactggtt
    22441 aaaagaccat acctaaaaga atgaaagcga tcacctatca ttgtggtatt acagatcgag
    22501 ttattgtcct tgacaaaaag tgaccacacg tgataatata gttatgttct gtttcggcag
    22561 attctttttt taagtaaaaa agtgaccaca cgtttattaa ttgtgtagag ctactttgtt
    22621 aaatattggc taatatttcg ccaaagctat gtcactattt cacatgataa acgattacct
    22681 aggagaaaaa acgaatggaa acaattttag aagtcaaaca tctcagtaaa atttttggca
    22741 aaaaacaaaa agcagctctt gagatggtaa agactggcaa aaataagagt gagattttta
    22801 agaaaacagg cgctactgta ggtgtctatg acgctagttt tgaggtcaaa aaaggtgaaa
    22861 tctttgttat tatggggcta tcaggaagtg ggaaatcaac ccttgtccgc atgctaaatc
    22921 gtttgattga accttcagca ggatctatct tgctggaagg taaagacatc tcaaccatgt
    22981 cagcagatca gctgcgtgag gtgcgccgcc atgacattaa catggttttc caaagctttg
    23041 ccctctttcc tcataaaacc attttggaaa ataccgaatt tggtttggaa ttacgtggcg
    23101 ttcccaaaga agaacgccag cgattggcag aaaaagccct tgataattca ggcctattag
    23161 attttaaaga ccagtaccca aaccaactat ctggtgggat gcagcagcgt gttggcctag
    23221 cccgtgcgct agctaatagc cctaaaattc tcttaatgga cgaggcattc tcagcgcttg
    23281 atcctttgat tcgtcgtgag atgcaagatg aattacttga tttgcaagac agcatgaaac
    23341 aaaccatcat ctttatcagt catgacttga atgaagcttt gcgtattggt gatcggattg
    23401 ctttgatgaa agacggacaa attatgcaaa ttggtactgg tgaggaaatc ttgactaacc
    23461 cagccaatga ctttgtgcgt gaattcgttg aggatgtgga ccgttctaaa gtcttgacag
    23521 cacaaaatat catgatcaaa ccgttaacaa ctactgttga attagatgga cctcaagttg
    23581 ccttgaaccg tatgcacaac gaagaggtgt ctatgttgat ggcgacgaat cgccgccgcc
    23641 aattagtcgg tagtttgacg gccgatgccg ctatagaggc gcgcaaaaaa gggttaccgc
    23701 tatcagaagt gattgatcgc gatgtgagaa ctgtctcaaa agatactatt attacagata
    23761 ttttgcctct tatctatgat tcatctgctc cgattgcagt gacagatgat aataatcgtc
    23821 tgctaggtgt cattattcga ggacgagtga ttgaagcctt ggctaatatc tcagacgaag
    23881 accttaacta aaaggagtga tgcattttgg aaacgatatt acaaacaaaa ttaccagtag
    23941 ctcaactggt ggagcagcta acggagtggc taactaaaac tttctcaggt ctatttgata
    24001 ttatgcaagt agttggtagc tttttgatgg attggatgac caaaacactt ttatttatcc
    24061 acccgttact ttttattgtt ctagtaacag caggggtgtt tttccttgct aagaaaaaat
    24121 ggcctttgcc aacctttacc ttgttaggat tgctctttat ttacaatcaa gggttatgga
    24181 agcagctaat gaataccttt actctggtgt tagtagctag tttgatttcg gtacttattg
    24241 gtattccgct ggggatttgg atggctaaaa atgccactgt ccgtcagatt gtgaatccta
    24301 ttttggactt tatgcaaacc atgccggctt ttgtttactt gattcctgcc gttgcctttt
    24361 ttggtattgg tatggtgcct ggagtgtttg cctcagtcat ctttgccttg cctccaacgg
    24421 ttcgttttac aaatttggcc attcgtgaca ttccaacaga attgattgag gcctcagatg
    24481 cctttgggtc aactgggaaa caaaagctct ttaaagtgga attgccttta gctaaaaata
    24541 ctattatggc aggtgttaat cagaccatga tgttagccct ttccatggta gtaacaggct
    24601 ccatgattgg tgctcctggc ttaggacgtg aagtcttgtc agcccttcaa cacgctgatg
    24661 ttggtag
//
LOCUS       DAUVUY010000017        23864 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.017, whole genome shotgun sequence.
ACCESSION   DAUVUY010000017 DAUVUY010000000
VERSION     DAUVUY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23864
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.017"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..524)
                     /locus_tag="VOX58_000648"
     CDS             complement(<1..524)
                     /locus_tag="VOX58_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HES5877899.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSIEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSA"
     gene            complement(594..1307)
                     /gene="rsmG"
                     /locus_tag="VOX58_000649"
     CDS             complement(594..1307)
                     /gene="rsmG"
                     /locus_tag="VOX58_000649"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HES5877900.1"
                     /translation="MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            1457..2014
                     /locus_tag="VOX58_000650"
     CDS             1457..2014
                     /locus_tag="VOX58_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HES5877901.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            2061..2957
                     /gene="htpX"
                     /locus_tag="VOX58_000651"
     CDS             2061..2957
                     /gene="htpX"
                     /locus_tag="VOX58_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HES5877902.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMINALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            3191..3724
                     /locus_tag="VOX58_000652"
     CDS             3191..3724
                     /locus_tag="VOX58_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HES5877903.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            3991..4677
                     /gene="covR"
                     /locus_tag="VOX58_000653"
                     /gene_synonym="csrR"
     CDS             3991..4677
                     /gene="covR"
                     /locus_tag="VOX58_000653"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HES5877904.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            4683..6185
                     /gene="covS"
                     /locus_tag="VOX58_000654"
                     /gene_synonym="csrS"
     CDS             4683..6185
                     /gene="covS"
                     /locus_tag="VOX58_000654"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HES5877905.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHGVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            6400..6894
                     /gene="nrdR"
                     /locus_tag="VOX58_000655"
     CDS             6400..6894
                     /gene="nrdR"
                     /locus_tag="VOX58_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HES5877906.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            6878..8053
                     /locus_tag="VOX58_000656"
     CDS             6878..8053
                     /locus_tag="VOX58_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HES5877907.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGDLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            8054..8956
                     /gene="dnaI"
                     /locus_tag="VOX58_000657"
     CDS             8054..8956
                     /gene="dnaI"
                     /locus_tag="VOX58_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HES5877908.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLPDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            9019..10329
                     /gene="der"
                     /locus_tag="VOX58_000658"
     CDS             9019..10329
                     /gene="der"
                     /locus_tag="VOX58_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HES5877909.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDEIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            10536..13634
                     /locus_tag="VOX58_000659"
     CDS             10536..13634
                     /locus_tag="VOX58_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HES5877910.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASNTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMKDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNENRASMSIEEIKEILGLN
                     TH"
     gene            13877..14479
                     /locus_tag="VOX58_000660"
     CDS             13877..14479
                     /locus_tag="VOX58_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877911.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            14519..15847
                     /locus_tag="VOX58_000661"
     CDS             14519..15847
                     /locus_tag="VOX58_000661"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HES5877912.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDTALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            15893..16375
                     /locus_tag="VOX58_000662"
     CDS             15893..16375
                     /locus_tag="VOX58_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HES5877913.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            16487..18061
                     /gene="mltG"
                     /locus_tag="VOX58_000663"
     CDS             16487..18061
                     /gene="mltG"
                     /locus_tag="VOX58_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HES5877914.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAARRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            18134..18616
                     /gene="greA"
                     /locus_tag="VOX58_000664"
     CDS             18134..18616
                     /gene="greA"
                     /locus_tag="VOX58_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HES5877915.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(19236..20159)
                     /gene="yidC"
                     /locus_tag="VOX58_000665"
     CDS             complement(19236..20159)
                     /gene="yidC"
                     /locus_tag="VOX58_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HES5877916.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMAFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(20241..20519)
                     /locus_tag="VOX58_000666"
     CDS             complement(20241..20519)
                     /locus_tag="VOX58_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HES5877917.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGK"
     gene            20780..21517
                     /locus_tag="VOX58_000667"
     CDS             20780..21517
                     /locus_tag="VOX58_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HES5877918.1"
                     /translation="MVITSKTNQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            21556..22056
                     /locus_tag="VOX58_000668"
     CDS             21556..22056
                     /locus_tag="VOX58_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HES5877919.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            22071..22760
                     /locus_tag="VOX58_000669"
     CDS             22071..22760
                     /locus_tag="VOX58_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HES5877920.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            22938..23180
                     /locus_tag="VOX58_000670"
     CDS             22938..23180
                     /locus_tag="VOX58_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HES5877921.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            23358..>23864
                     /gene="murI"
                     /locus_tag="VOX58_000671"
     CDS             23358..>23864
                     /gene="murI"
                     /locus_tag="VOX58_000671"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HES5877922.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSVQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSL"
BASE COUNT         7719 a         4069 c         4858 g         7218 t
ORIGIN      
        1 gccgacacag ctagaaaaat actattgaag ataccatttt tccacccgaa gcgggggata
       61 aagtcaatca tgataactat cgcagcaaag gcttctatac taaaggttac tttataagca
      121 aaaaaaaggt agtgctttaa atctttgcta tcacctcgat ttaacgctga ttggagcaag
      181 gtttgatcgc tcaatcgcat ttttcgcttg agggcaaaag tactcacagc tattaacgtc
      241 accaagccaa gaccaccaat ctgcattagt gccatagcaa tggtttgtcc gataccgtta
      301 tacacttcag cgacgggaac aacagaaagt ccagtcacac aaaccatgga aaccacatta
      361 aagaaatgat caagatagac ggtattagga ccattttgat aatgggtaaa gggcatggat
      421 aacaagagag tcccaatcag aatcacaatg gcaaaactaa atgttaagcg ctgtgtgact
      481 gaaagagatt tgataaaaga gcgtttcatg tttcctccag acataatatc tcctactata
      541 ataacaaaaa taagttaaaa aagctaagtt tcatcttagc ctttggtact attttataga
      601 ggttttttgt taggcaagcc agcttttctt ggatacttgt taggtgtttc ctttttcttc
      661 tcaacaatgg tgatgaagcg agaatctcca tttggcaatt ggtagctatg gtttttaatg
      721 actttaccaa aaagaagaca aagagcattc ttagcttctt ctaactcttg gtcagcagct
      781 tgtgccttta aggcgattaa ttttcctcca atttttaaaa aggggatggt taattcagat
      841 agaacctgca tccgagcaac cgctctggca gtaacgacat caaattgacc tcgaaaggcc
      901 ttgtcctgtc cgaaatcttc tgctctacca tgaaaaaaat ggacattttc caagcccaat
      961 tcctgagcaa ggagtgtcag aaaagaaatc cgcttattaa gagaatcaat aatggtaacc
     1021 tcaaggttcg ggaaaagaat tttcatgggt aggctaggaa aaccagctcc agctccaatg
     1081 tccaataatt tgattggttc attagctaaa aagccctgta aaataggagc gatggaatca
     1141 taaaaatgct tgaggtaaac ctcattttct tctgtaatag ctgttaaatt aattttagtg
     1201 ttccattcca ctagtaattt aaagtaggtg tcaaattgtt ctttttgttt cgatgataag
     1261 gaaaaaccat cttcctctag tgttcggtaa aagtcttggg gtgtcatttg tatcctctgt
     1321 ttttgatagt tgttaccatt ttatcataaa atagcccata acaaaatcca ataaatcatc
     1381 agaaataaca cctcattact tgctattagc ctagaaattg ctataattga gacacaaaga
     1441 ttaaaggaga cccactatgc caacagtttt gattatctta gtcgttttag gagtgcttgc
     1501 gctatggtta atgattagtt acaatagcct ggttaaatct cggatgcaca caaaagaagc
     1561 ttggagtcag attgatgtgc aattaaagcg tcgtaatgac ttgattccaa atcttattga
     1621 aaccgtaaag ggatatgcta gctacgagca aaagacattt gaaaaaatca ctgatttgcg
     1681 tgcgcgtgtt gcgaatgcct caactcctca agaaaccatg gcggcttcta acgaattgag
     1741 taaacaagtg accagtttgt ttgccgttgc tgaaaattac ccagacttaa aagctaacga
     1801 aaacttcttg aaattacaag aagagttgac caatacggaa aataaaatct catattctcg
     1861 tcaactctat aattcaacaa cgtctaatta caaccttcaa ttagaatctt tcccaagcaa
     1921 tatcgctggt aaattatttg gttttaaacc aagtgaattc ttacaaacac cagaagctga
     1981 aaaagaagtt ccaaaagttg aatttaactt ttaaatatga aattatcatc tagtaggcaa
     2041 tcataagaga aaggagaatt atgctttatc aacagatttc acaaaataag caacgaacag
     2101 ttgttttatt agttgttttc ttcgctctct tagcacttat tggagcttca gcaggctatc
     2161 tgctgttaga taattatgcc atgggcttgg ttcttgctct tgtcattggg gtgatttatg
     2221 ctaccagtat gatttttcaa tccaccagtc ttgtaatgag tatgaacaat gctagagaag
     2281 tcacagaaaa agaggctcca ggcttcttcc atattgtcga ggatatggct atggtggctc
     2341 agattccgat gccgagagtt tttattattg aagatccttc tttaaatgct tttgcgacag
     2401 gatctagccc tcaaaatgct gctgttgcag caacgacagg tttacttgaa gtcatgaatc
     2461 gtgaagaact tgaaggtgtt atcggccatg aaattagcca catccgaaat tatgatatcc
     2521 gtatttcgac tattgctgta gctttggcga gtgctgttac cgttatttct agtatcggtg
     2581 ggcgtatgct atggtatggt ggcggttctc gcaggcaaag agatgacgga gatgatgatg
     2641 ttttaagaat cattacatta cttttgtcgt tattatcttt gcttttagcg cccctagtag
     2701 caagtttgat tcagttggct atttctcgtc aaagagaata tctagctgat gctagttctg
     2761 ttgaactaac aagaaatccc caaggtatga ttaacgctct tgaaaaattg cagttatctc
     2821 agccaatgaa gcatcctgtt gatgatgcta gtgcagcctt gtatattaat gagcctcgca
     2881 aaaaaaggag cttcagttca ttattcagca cccatcctcc tattgaggag aggattgaaa
     2941 ggctaaaaaa catgtgagcg tttggggttt ttgctcaaac ctcacttttt tatgtctttg
     3001 ggcagataat ctaaaatggc aggaattatc aaaaatatct gaacaaaaag aaaaaaatga
     3061 gtgaaacaat ggttaaaaat agaatgattt aaatcttaaa attaaaaaac gtcagaaaat
     3121 aaatgcaaaa ctattgacaa atcttttaga tagtagtaaa aataaatgag agttttatta
     3181 ggagaagatg atgttagcta tttccgaaat cagaaaacac ccagacggtc tctcgtttga
     3241 tcgtttatgt gatgtcaaaa gtatgttact tgagcgtgac caacagatta ttgacataaa
     3301 ggcagttaaa gctgtcggta atgttcgcta tgataagggg ctttatctgt tagattatca
     3361 attgagttac gaagtgatat taccgtctag ccgttctatg gttccggtct gtttaagtga
     3421 agtccaacac attcaagaat tatttataga ggcgacagat ttagctgata agaaagagtt
     3481 ggttgaagac aatctggtat tagttttaga caaagacgct attaacctcg aagaaagtat
     3541 tgtggataat atcttacttg ctattccgct acaggtcttg acagaggaag aaaagaaatc
     3601 aaaagagttg ccagcaggtc aaaattgggc agttttgaca gaagaagact accagtgtct
     3661 aaaagaagaa aagcaaaaag aaaataatcc ttttgctagc ttgcaagggt tgtttgatga
     3721 ataaaactga atattaaaga tgtctgtaat ttttatttga aaaaacagct attctttgat
     3781 atactcttta gagaatatgg ttacttatct aaagtagaag gtattttgac catagagggc
     3841 aaagaaggta atgactatta gatgcttcat attttattcg ctagaagact atttgaccat
     3901 tagaactgaa atagtctagg atatgagatg aatttcatag ttttctttct gggagaaaaa
     3961 gatagattaa gaggataagg gttggtataa atgacaaaga aaattttaat tattgaagat
     4021 gaaaagaatc tggctagatt cgtttctctt gagctgcaac atgagggtta cgaagtcatt
     4081 gttgaggtca atggtcgtga agggttagaa actgctttgg aaaaagagtt tgatttaatc
     4141 ctgcttgact taatgttacc agagatggat ggttttgaag tgacccgtcg tttgcaaacc
     4201 gaaaaaacaa cgtatatcat gatgatgact gcgcgtgatt ctattatgga tgtggttgca
     4261 ggtttagacc gcggtgcaga cgactatatt gttaaaccgt ttgccattga agaactactt
     4321 gcccgtattc gtgctatttt ccgccgtcaa gatattgaat ctgagaagaa agtgcctagt
     4381 caaggcattt atcgagatct agttttaaat ccacaaaacc gttcagttaa tcgtggcgac
     4441 gatgagattt ctctcactaa acgtgaatat gatttgctta atattttgat gactaatatg
     4501 aatcgtgtca tgacacgtga agaattattg tcaaatgttt ggaaatatga tgaagccgtt
     4561 gagactaatg ttgtagatgt ctatattcgt tatctccgcg gcaaaattga cattccaggc
     4621 aaggaatctt atatccaaac agtgcgtggc atgggatacg ttattcgtga gaaataagtc
     4681 atatggaaaa tcagaaacaa aaacagaaga aatataaaaa ttcgttacca aaacgactat
     4741 ctaatatctt ttttgttctt tttttctgca ttttctctgc ctttacactg attgcctata
     4801 gttcaacaaa ctatttctta ttgaagaaag aaaagcagtc agtctttcaa gctgtaaata
     4861 ttgttagagt tcgtctttct gaggtggact ctaattttac attagagaac ttagcagaag
     4921 ttttgtacaa aaacgataaa acacatctga gaattgatga cagaaagggc agtcgagtca
     4981 ttaggagtga gcgcgatatc acaaatactc tagatgcgaa tcaagatatt tatgtctata
     5041 acattgataa gcagatgatt tttaccacag ataacgaaga atcatctcct ggcttgcatg
     5101 gtcctatcgg tcgggtgtat cacgaccata tcgaagatca gtatcgtggg ttttccatga
     5161 cacaaaaggt atattctaat cggactggaa aatttgtggg ctatgttcaa gtctttcatg
     5221 atttaggcaa ttattatgtc attagagcaa gactgctgtt ttggctacta gttgttgagt
     5281 tatttggcac aagcttagcc tatttaatca tcttaattac tacgcggcgc tttttgaagc
     5341 ctttacacaa tttacatgga gtaatgcgta atatctctga aaatcctaat aatttaaacc
     5401 tgcgctcaga tatttcgtca ggagatgaaa tcgaagaatt gtctgttatc tttgacaata
     5461 tgttagacaa attggagaca catactaagt tgcaatcacg ttttattagt gatgtcagcc
     5521 atgaattacg aacaccggtt gcgattatta aagggcatat tggtctctta caacgttggg
     5581 gtaaagatga cagtgatatt cttgaagaaa gtttgacagc aacagcgcat gaggctgacc
     5641 gtatggcaat catgattaat gatatgttag acatgattcg cgtacaaggc tcatttgagg
     5701 gacatcaaaa tgatatgaca gttttggaag attctattga aactgttgtt ggtaatttta
     5761 gagttttaag agaagatttt atctttacat ggcagtcaga aaatccaaaa acgatagccc
     5821 gtatttataa aaatcatttt gagcaggctt tgatgattct tattgataat gctgtgaagt
     5881 attcccgtaa agaaaagaaa atcgcgatta acctttcagt gactggcaaa caagaagcta
     5941 ttgttagagt tcaagataaa ggcgaaggaa tttctaaaga agatattgaa catatctttg
     6001 aacgctttta tagaacggat aaatcacgta atcgaacaag tactcaggct ggattaggga
     6061 ttggcttgtc tattctcaag caaattgtag atgggtatca tttacagatg aaggttgaaa
     6121 gtgaattaaa tgaaggttca gtgtttatct tacatattcc tttggcccag tctaaagaga
     6181 gttagagtag cttaaggcat tgttaatgtg acaggtatag tatgtaatca ttagataata
     6241 gtagcagtct tttgccacaa ctggttagaa gctgatgcta tttttttagc aatgatggat
     6301 gacatgaaag aaatactgtc aatcgcgatg ccttttgtta tccttgataa tatggtaaaa
     6361 tagtgtatta gattaatttg atagaattgg ggtgtttgcg tgcgttgtcc aaaatgtaat
     6421 taccataaat cgagtgttgt tgacagtaga caagctgaag acggcaacac cattcgtcgt
     6481 cgaagagaat gtgaacagtg tcatactcgc tttactacct ttgagcgtgt tgaagaactc
     6541 cctttattag tcatcaaaaa agatggcaca cgagagcaat tttctagaga taaaatatta
     6601 aatggggttg tccaaagtgc gcaaaaacga cctgtctcga gcacagatat tgaaaatgtc
     6661 atttctcgta ttgaacaaga agttcgaaca acctacgaaa atgaagtgtc aagtactgct
     6721 attggtaatt tagtgatgga tgagttggcc gaacttgatg aaatcacata tgtacgcttt
     6781 gctagtgtct acaagagctt caaggacgtt gatgaaatcg aagaactttt gcaacaaatt
     6841 acgaatcgcg tacgcggaaa gaaaaaacgt ttaaataatg atgaaaccaa ttgatacttt
     6901 tacatatcta aaacgcaaca aagtaactct tgattcggtg actttaatac agttgtactt
     6961 tccaattatt ggaagtgacg cagtctctat ctatcaatac tttattcact tttttgacga
     7021 tggtttacaa cgtcataaat tcagcgatat tttaaatcac ctacagtttg gtatgaaacg
     7081 ttttgaagat gctcttgcca tattgacagc aatggaatta gtgagtgtgt atcagctatc
     7141 agatacatat ctcattacgt tgcaccagcc gcttagccgt gacttgtttt ttcaacatcc
     7201 agcttattct cgattattag aacaaaagat tggagaggta gctgtctctg agttgcaggt
     7261 aactgtccca agtcaagcaa gaaatatttc taagcgattt tcggatattt ttggggtgca
     7321 aggagatttg acgaacgtac ctcaaaagcc acaaaagaat tttgaccttt caagtttcca
     7381 gcaattaatg gttcgtgatg ggttacaatt tgaagataat caaaaagata tcatcagcct
     7441 ttacagtatt gctgaacagt atgatatgac gtggtttgat acttatcaaa tagctaaggc
     7501 gacggctgtt aatgggaaaa ttcgacctga acgcttattg gctaaaaaaa atcaaagcat
     7561 gacaaaacca agcaaagaaa atttttcaca ggctgagcaa attattttgc gagaagcaaa
     7621 gcaagacagt gctttagtct ttttggaaaa aattaaaaag gctagacgtg caacgattac
     7681 taaggatgaa cgcattttat tgcaaacatt ggccaaaatg aattttcttg atgatgttat
     7741 caatgtcatg gtgctttata cctttaataa gacaaaatct gctaatttac agaagagtta
     7801 tgtcttaaaa atggccaatg attttgccta ccaaaaggtg tcaacggctg aagaagctat
     7861 agtggttttg cgagccttca cggatcgtca aagtcgtcgg caatcaaaag taaaaacaag
     7921 tcaaagcaat gtgcctaagt ggagcaatcc tgattaccaa gagacaacaa gtcaagaaga
     7981 gcaggcgaaa ctagatcagt tcaaacaggc tgctttgaag aggttagaaa acctaggaaa
     8041 agggggtgac tagatggaaa agattggaga aaccatggcg aaattgggtc aaaacactcg
     8101 tgttaatagc gaccagttga ttcaaaccat tttagcggat cctgaagtag caagcttcat
     8161 cagtcaacat cacttatctc aagagcaaat taaccttagc ttatctaagt tcaatcaatt
     8221 tttggtggaa cgccagaagt atcaactcaa agatccctcg tatattgcta agggctatca
     8281 gcctattcta gctatgaatg agggctacgc tgatgtgtct tatttggaaa ccaaagaatt
     8341 ggtagaagct caaaaacagg cagctatctc tgaacggatt caactggtca gtttgccaaa
     8401 atcctatcgc catattcatt taccagatat tgatgttaat aatgcgagcc gtatggaagc
     8461 tttctctgct attttagatt ttgtggaaca atacccatca gcagagcaaa aaggcttata
     8521 tttgtacgga gatatgggaa ttggcaagtc ttacttgttg gctgctatgg cccatgaatt
     8581 gtctgagaaa aaaggtgtgt caacgaccct tctacatttt ccgagctttg ccattgatgt
     8641 aaaaaatgcc atttcaaacg gctctgttaa agaagaaatc gatgcggtca aaaatgtacc
     8701 agtgctcatt ttagatgata ttggtgcaga gcaagccact tcttgggtcc gtgatgaggt
     8761 gttgcaggtg attttacaat accgtatgtt agaggaattg ccaaccttct ttacctctaa
     8821 ttacagcttt gcggatttgg agcgaaaatg ggcaaccatc aaaggaagtg acgaaacttg
     8881 gcaggccaaa cgtgtcatgg aacgcgtgcg ttatttggct agagagtttc atttagaagg
     8941 ggctaaccgc cgttaagttt gagatagcaa tcaagaagaa tagctacacg tatgagtgag
     9001 tgaaagaaaa ggagaatcat ggttttacct accgttgcca ttgtgggccg tccaaatgtg
     9061 ggcaagtcca ccttatttaa tcgaatcgca ggagagcgca tttcaattgt agaagacgtt
     9121 gaaggggtta ctcgtgaccg tatttatgct actggggagt ggcttaaccg ccaattctca
     9181 ttgattgata caggtggtat tgatgacgtt gatgccccgt ttatggagca aatcaagcac
     9241 caggctcaaa ttgctatgga agaagcggac gttattgtct ttgtcgtttc tggtaaagaa
     9301 ggggtgaccg acgctgacga atatgtgtct aaaattctgt atcgaaccaa taccccagtc
     9361 attttagctg ttaataaggt tgataatcct gaaatgcgta atgacattta tgatttttat
     9421 tcacttggtt taggggatcc ctatccagtt tcttctgttc atggtatcgg aacaggggat
     9481 gtcttggatg agatcgttga aaacttgcca gtagaagagg ctgaagaaaa tgatgatatt
     9541 attcgcttca gtttaatcgg tcgtcctaat gttgggaaat ctagcttgat taacgctatt
     9601 ttaggtgagg accgtgttat cgctagccct gtcgcaggga caacacgtga cgctattgac
     9661 acgcatttta cggatgctga cggtcaagaa tttaccatga ttgacacggc tggaatgcgc
     9721 aaatctggca agatttacga aaatacagag aaatattctg tcatgcgtgc catgcgtgcc
     9781 attgaccgct ccgatgtggt cttgatggtt atcaatgctg aagaaggtat ccgtgaatac
     9841 gacaagcgta tcgctggctt tgcacacgaa gctggtaaag gaatgattat cgtggttaac
     9901 aaatgggata ccattgacaa ggataatcat accgttgcta agtgggaagc cgatattcgt
     9961 gaccaattcc aatttttgac atacgctccg attatttttg tgtcagcttt gacgaagcag
    10021 cgcctcaaca aattgcctga cttgattaaa cgtatcagtg agagtcagaa taaacgtatc
    10081 ccatctgctg tcctcaatga tgttatcatg gatgctattg ctattaatcc aacgccaacg
    10141 gataagggta aacgtttgaa aattttctat gccactcaag tgtcagtaaa gccaccaaca
    10201 tttgtcgtct ttgttaacga agaagaattg atgcacttct cttatctacg cttcttggaa
    10261 aaccagattc gcgcagcctt tacctttgaa gggacaccga ttcatctgat tgctcgcaaa
    10321 cggaaataag aaatgaacat caactaaaat gctgacctaa gaaataatct catcattgat
    10381 aaggactgtg ttgtgtatgt tacaggagac agtccttttt ataatacctt taagggtgta
    10441 gacggaagat tagtagcaaa caaagaaaaa ggattttgac aagaaatatc agtcggtttt
    10501 tgctataatg aaaggataag caaaaaggtg gaaaaatgat ggctagatta attccaggac
    10561 gagtaaggaa tgaaggtatt aagctttatg agcaaggttt agtctcattc caagatgata
    10621 ataaaggcat tcttcaaata gaagtggaga cttatcaggt tcaatatgga gcagatgatg
    10681 aggatatcac ttgtcaatgc gacacatttc atatgaaaca ttattgcaag catattgcag
    10741 cagtcgagta ttttttaaaa aatgatcaaa aaggtaagct ttttttaaaa cagttaacaa
    10801 atcaaacaaa aatcaaagaa acaacaaaga aaatgacatc ttttggtagt ctctttttag
    10861 atagcttagc catgaatgaa gatgattctg ttaaatacag gctgtctgct cttggtagtc
    10921 gaagcccttt ttcgtcagat tactggtgga gtctgaaaat caatcgttta ccagatgatc
    10981 gttcttatgt gattcgagat attaaaggct ttttgcagct tattaaaaaa gaaggctttt
    11041 atcagattgg aaaaaattat tttgaacaac tttcatggct gcagtttgac ccatccagtc
    11101 aagaccttat tgagttttta tggcgcttag catctaatac tgacaaaggg gacaacgaga
    11161 acatttttcc aaatcatgca aggcatttgc gcttacctag tggttttttt gaagaaggta
    11221 ttcactattt gacgtcactt tatgacttta cttttgaggg gccttctcaa acttatcatc
    11281 atctttttgt ccgttctttg gaagcagagg caggccttta tgagtttaaa gtggaagtgc
    11341 atcgcaaatc tattgaattg caaattgctg aaaaaaatgt gcaatacctc tttgataatg
    11401 actatcttct ttatcaagat accttttatc acttgacgtt aaagcaacga aaaatggttc
    11461 aggctattcg tagtctgccc attgaggccg acttggctaa acatattcat tttgatttgg
    11521 atgatcatgc gaaattagct gcaagcttat cagattttaa acagatcggt ctagtagagg
    11581 ctcctaaaag ctttgctatt agagactttg aggtgacttt tcaatttgat ttgttaaatc
    11641 gagatgaaat cagttgtcag cttatgtttg attatggcaa ttaccaagtt agtgacaaag
    11701 caagtttaga agcacttcct tttgctagtc atttaaaaaa agaagaaaaa atcaatcgtt
    11761 ctttacttgc ttttggcttc tcgccacaat tttactcaaa aaagcgacta acaagtgcaa
    11821 aagagctcta tacttttttt gaagaaacag ttccatgttt tgagcgctta ggtaatgttg
    11881 cgttgagtac agctatccaa gcattacaag tgaaagaaat gcccaagatc gctattagac
    11941 gcaatcaggg attactagat atttcgtttg atttttcaac cattattgaa aatgatattg
    12001 accaagctgt tacagctctt tttcaaaata atccttattt tgtgagtcaa acaggtcaat
    12061 tagtggtttt tgatgatgaa actcaaaaag ttagcaaaag tttacaagaa ttaagagcaa
    12121 gacagctaaa aaatggtcac ttgcaactgg atggcattag agctctacaa gtatccaaac
    12181 tttttgaagg catgacatct gttcattttt caaaggaatt agaagagtta gcctatcatt
    12241 tgcagcatcc tgaaactttt tcaataaaac cgttaccagt taaggctcaa atgcgagatt
    12301 atcaacgcaa cggtgttcag tggttatcaa tgctcaatca ttatggcttt ggtggcattt
    12361 tagcagatga catgggactt ggaaaaactc tccagacact tgctttttta gctagtcacc
    12421 tgaaatcaga tagtaaagtg ctcattttat ctccatctag tcttatctat aattggtttg
    12481 atgaatgtca aaaattcaca cctcagctag atgttgtagt ttcttacgga cttaaacaaa
    12541 tcagagatca aatcatcgaa gaaggtcatc aaataaccat aaccagctat tcctccttta
    12601 gacaggattt tgagacctat caagcgtttc actacgatta tcttatctta gatgaagctc
    12661 aagtgataaa aaatgcgcaa actaagattt cacactgttt acgtgcgttt aatacagcca
    12721 attgttttgc gctttcgggc actcctattg aaaataaaat gcttgaaatt tggtccattt
    12781 tccaaattgt tttgccaggg ttgctaccga ctaaaaaaga gtttttaaaa ttaacagctg
    12841 agcaagtatc gcgctatatc aaaccctttg tgatgcgtcg taaaaaagaa gatgtcctac
    12901 cagaattacc tgatttaatt gaaactaatt attcaaacga gatgacagat gaacaaaaag
    12961 ctatttactt agcgcagtta aggcagatga aagatcaaat tcgaaattct agtgatgtgg
    13021 atattagccg tcaaaaaatt gagattttat ctggtatcac tcgattgagg caaatctgtg
    13081 acactccaag tctctttatg gattaccaag gcaaaagtgg taagctagat agtttgcgaa
    13141 tcttattgac acaaattaaa gaaaatggtc atcgggcttt gattttttct caatttagag
    13201 ggatgctgga cttggccaag caagagatga ctgctctagg attgacctct tatcaaatga
    13261 ccggatctac ccctgccaat gaacgtcaag aaatgactag agcctttaat aacggttcca
    13321 aggatgcttt tttgatttcc ctcaaggcag gtggagttgg aatcaacctt acgggagcag
    13381 acacagtgat tttaatcgat ttatggtgga atcctgctgt tgaaatgcag gcaatcagtc
    13441 gcgcctaccg tattggtcaa aaagaaaacg ttgaagtata tcgcttaatc actcgaggga
    13501 cgattgaaga aaagatttta gagcttcaag aaagtaagcg caatttggtg actacagttt
    13561 tggatggcaa tgaaaaccga gcaagtatgt ccattgaaga aataaaagaa attttggggc
    13621 taaacactca ttaatcttcc ttgattttag tgacattaaa aaccctttaa agaagatgac
    13681 accaaaagca ccttatataa cctcgttaaa acgcttcttt ttgaaatatc tttattgata
    13741 tagcgattgt catttgctag taatgattaa aacatttaga aaaagacaag tcatagaatc
    13801 atcaaggaat gctttgaaaa ataacagtat ttaagttata atagaggatg ctacaagaag
    13861 ggagaatcaa aaaaggatgg taaaaaatca gtttccttta gttgcagatg gcattgctat
    13921 tagtgatcca gcaaagcaaa tggctctgta tgaaaatgaa gatctcatta ccaacatccg
    13981 tggctattac caggacaagg agtatgatga tattgctaga aatgaggagt ttactgctaa
    14041 agcaacttct cggcaggccc cttctagtaa gcgcttttgt agcaatgatg aaaaacacca
    14101 ttatgtcaaa gaggcgcgtc aaaaagcaaa gcaagatctc aaggaaaaaa gacaggctta
    14161 tcttgccaaa gaaatggcct atgttcctaa acaggtttct aaaaagcaac agcctgcgga
    14221 ttcttctcct tctcaaaagc aagcaacaac agaaatgagt cgttttacaa aaaagcttca
    14281 tcaagacaac tatattttag ctgagttgcc aaaagaatat aaagagccaa aaaatcttcc
    14341 tcaacaagga actacaaaaa aaaataatta cgatttttta aaatccagtc aaatttacaa
    14401 taataaagaa atgagacaac aacgagaaaa aacaattgcg caagagctta atcttagtag
    14461 gtttgaggat cttccttaat aaatctactc agcgtatgca gatgacaaag gagttaagat
    14521 gtcaaaaacg tatcatttta tcggaattaa agggtcagga atgagtgcct tggcccttat
    14581 gttgcatcaa atggggcata aggtgcaagg tagtgatgtt gaaaaatact atttcactca
    14641 aagaggattg gaacaagcgg gcatcactat cttacctttt agcgaggaca acattacgcc
    14701 agatatggaa cttatcgttg ggaatgcctt tcgtgaaaac aacaaagaag tggcctatgc
    14761 tttgagacac cagattcctt tcaaacgtta tcatgacttt ttaggtgatt ttatgaaatc
    14821 atttatcagt tttgcagtag caggtgctca tggtaaaact tctacaacgg gtttattgtc
    14881 acatgtcttg aaaaatatca cagacacatc ttatttaatt ggtgatggta ctgggcgtgg
    14941 atctgctaat gcacagtatt ttgtttttga atcagacgaa tacgaacgcc attttatgcc
    15001 ttatcaccca gaatactcta tcattactaa tattgatttt gaccatcctg attatttcac
    15061 aggaattgct gatgtgcgaa acgctttcaa cgattatgct aagcaggtca agaaagcttt
    15121 gtttgtttat ggtgaagatg acgaactaaa aaagatcgaa gcacctgcac ctatttacta
    15181 ttatggtttt gaagagggca atgattttat cgcttacgac attacgcgta ctacgaacgg
    15241 atcggacttt aaggtaaaac accaaggaga ggtcattggt cagtttcacg tacctgctta
    15301 tggaaaacat aacattttaa acgctacagc agtaattgca aacttgtttg tagccggcat
    15361 tgatacggct ctagtggcag accatcttaa aactttttca ggtgtgaaac gtcgttttac
    15421 agagaaaatt atcaatgaca ctattattat tgatgacttt gctcatcatc caaccgaaat
    15481 tgtggcaaca attgatgcgg ctagacaaaa atacccaagt aaagaaattg tagcaatatt
    15541 tcagccacat acttttactc gcactattgc cttattagag gattttgctt gtgctttaaa
    15601 tgaagctgac agtgtttatt tagcccagat ttatggttct gcacgtgaag tagataaagg
    15661 tgaagttaag gttgaagatt tggctgctaa gattatcaaa ccatcacaag tagtaacggt
    15721 ggaaaatgtc tctcctcttc ttgatcacga taatgctgtt tatgttttta tgggagctgg
    15781 agatattcag ctatatgaac attcttttga ggaattactt gcaaatttaa ctaaaaataa
    15841 ccaataacat gtcctgcctt aggcaggttt tctcattcta aaggagtttg atatgttgat
    15901 tagacaggtt caaggatccg atttggaagt tattgctacc attgaaagtg acaatttttc
    15961 accacaagaa gcaacgacaa gagcagttct tgaagagcgc atacggctga taccagatac
    16021 ttttttagtg gcacttatcg atcaggaaat tgttggctat atcgaaggtc cagttgtcac
    16081 tactcctatt ttggaggatt ctttgttcca cggggtgact aaaaatccta aaactggagg
    16141 ctatattgct attactagtt tgtccatagc taaacatttt cagcaacaag gggttgggac
    16201 agctttgttg gcggcactaa aagatttagt agtagcgcag caaagaacag gtcttatttt
    16261 gacttgtcat gattacttga tttcctatta tgaaatgaat gggtttatta accaaggaat
    16321 ttcagagtct caacatggtg gaaccctttg gtaccaaatg atttggaaac tttagaatca
    16381 cagctgattc aactgttggt ttgttaaaaa taagtaacaa atgcatcatt taattgcaat
    16441 taggatactt ttaagttata atggcggttg attatattta ggaggattgg ctttgaccga
    16501 ttttaaggat aaagaccaac aagatcagca gcgaagcttt aaagagcaga ttcttgctga
    16561 gttggaaaaa gcaaatcaaa tcagaaaaga aaaagaggaa gaactttttc aaaaagagtt
    16621 ggaagctaaa gaagcagcta ggagaacggc ccagctatat gctgaatata aaagacaaga
    16681 tgcttttcaa aaagagtcta tagcacataa caataagaca gctaaacact ttcaagctat
    16741 aaaaggtgcg gtaatgactt cagaagcgct taaaccgact ttactttctg aaaaagaaaa
    16801 ctcatcttta aaaacgacaa ataagagagt cgtgcaggca aatgagcttc aagagactgc
    16861 ctctaaagaa tctcaagtac cgttaactat tgagaaaggt cattcagtga gacgaaaatt
    16921 aagcaaacgc caacagactg agcgagctgc taaaaagatt tcaaccgttt tgattagttc
    16981 tattattata acccttttgg ctgttactct agcaggagca ggctatgttt atagtgcttt
    17041 aaatcctgtt gataaaaata gtgatgcctt tgttcaagtt gagataccat ctgggtcagg
    17101 caataaattg attggtcaaa ttcttcaaaa aaaaggttta atcaagaata gcactgtttt
    17161 tagtttttat acaaaattta aaaactttac aaattttcag agcgggtatt acaatctgca
    17221 aaaaagtatg agtctagaag aaattgctag tgctttacaa gaaggtggta cagcagaacc
    17281 taccaagcca tctcttggga agatcttgat tccagaagga tacacgatta aacaaatagc
    17341 taaagctgtt gagcataata gcaagggaaa gaccaaaaaa gctaaaacac cttttaacga
    17401 gaaggatttt ttggatttag tcacagatga ggcttttatt caagatatgg taaaaagata
    17461 tccaaaatta ttagcaacta tcccaactaa agaaaaagct atttaccgtt tggaaggtta
    17521 ccttttccca gcaacctata actattacaa agaaactacc atgagagaac ttgtagagga
    17581 catgctggca gctatggatg ctactttggt accctattat gataaaattg ctgctagtgg
    17641 taagacagtc aacgaggtat tgaccttggc ctctttggtt gaaaaagaag gttcaacaga
    17701 cgatgacaga cgtcaaattg caagtgtctt ttataaccgc cttaatagcg gaatggcact
    17761 acaatctaat atagctattt tgtatgcgat ggggaaactt ggtgagaaaa caaccttggc
    17821 tgaggatgct actattgaca ccaccattaa ttctccttat aatatttata ccaatacagg
    17881 tctgatgcct ggtccagttg ctagctcggg ggtttctgca attgaagcaa ccctaaatcc
    17941 agcctcaacg gattatttat actttgtggc caatgtccat actggtgaag tttactatgc
    18001 aaaaacattt gaagaacact ctgcaaatgt tgaaaaatat gtgaatagtc aaattcagta
    18061 aacacaaaca tggtgcttaa gcaccatgtt tgtgtttctg attcgttcaa aaaagaaaag
    18121 agagaaaaca attatggcag aaaaaacgta tccgatgacc ctaacggaaa aagaacaact
    18181 tgaaaaagaa cttgaagaat taaaacttgt gcgtcgtcca gaaatagtgg agcgtattaa
    18241 aattgcacgt tcgtatggag atctttcaga gaactctgaa tacgatgccg caaaagatga
    18301 gcaagctttt gttgaaggtc aaatttcaac gcttgaaact aagattcgct atgctgaaat
    18361 cattgacagt gatgctgttg caaaagatga agtcgctatc ggaaaaacag ttatcgttca
    18421 agaagttgga acaacagata aagataccta tcatattgta ggtgctgctg gagcagatat
    18481 cttctcagga aaaatttcaa atgaaagccc gattgctcaa gctttgattg gtaaaaaaac
    18541 aggtgacaag gttagaattg aatcaccagc agctacttat gatgttgaaa tcatcagcgt
    18601 tgaaaagaca aattaatcag atgaaaccat tatttgccta accactaagt gttttggttg
    18661 ctaatggctt ttctagtatc ttcaaatgag gttgactgtt aggaaggtca agtattaatc
    18721 gcattgatta tgagccaaga ggactataga tcaaagatcg ttttcgatgg ttatttaatg
    18781 ccaaaagaaa agaccagcac cttaatgtac tgaccccaaa aagttggaca ctatatttta
    18841 aggaagtgta tgatctaagg aaactgacac taatgattga ccgttttgca agactttatt
    18901 aatgatttct tgctttattt taaatgaata agtctgatac gtttccttcc ttaacactat
    18961 cgactccata ctgacccatc agtcaaacgg tatatctcaa acgactacgg tttactccaa
    19021 attgattggc tagttgaggc caagaaacac ctgatttttt ttaatgctaa atttctagct
    19081 tatcttcata gcttaatttc atacaaaaac accccaatcg ttagattttt ttgatatgtc
    19141 ccctttcact agatgagtcc agtaaaaggg ggcatatcgt tttttgtcta acttttgggg
    19201 tgcagttcat aattgtgcta ggttttttgt ctttcttaac gctttcgctg ttttccagca
    19261 ttacgattgg attttatttt tttaggcaag ttagcctgtt ccatgttttg tgaaggcgta
    19321 acgtccttac gagaagaagt tgattggtat gctttaggcg ggtttttagc gtattcttct
    19381 ttaatttgct tatgcaatcg aggttttaag aggtaggttg tgattaactg ttgaataata
    19441 ctaaagaaac caccaactag ccagtaaaga ccaacaccag cagggagtga gaaggacatg
    19501 aagatcatca tgataggcat ggtgtacatc atcgtcttca tttgctcacg ttgttcttct
    19561 gaaacggcca tcattgataa ccatgattgg aagaagtaaa gagcagcgat aattgctgtt
    19621 aacactaaac tacggctacc aagatcaata cccataaagg tgcttgttga tactccttta
    19681 gtgtattgag cggcaaaata catggcagaa aagaatggca tctggatgag aagaggtaga
    19741 catccaatac ctccaagagg attaatccca tgagcacgct gagcagccat taattctgtt
    19801 tgggcagcca ttttttcttc ctgactactt gcctgtttga tacgtttgtt gattggttca
    19861 aaaacaggtt tcaagaaggc cattttctca gattgataac ttgctttcca agattgatat
    19921 agacctagtg gtaagataag tgttctaaca ataatggtta cgatgatgat agccagtcca
    19981 taacctagtc cagcattgtt tgcaaagtaa tcgataaagt atgacatggg ttttccaaga
    20041 aattcccaaa tcataccttt gggatttccg tgagcgtctc ttcccacaca gcctgtcaag
    20101 gtgaatagaa ttgacagtgc tagtcctgaa aagaggatgc gatttaatgt tagtttcaat
    20161 gtgtttatcc tttaatttta atatacttat ctattttaca gtttttttca gaaattcaca
    20221 atagttctga aggccgattt ttacttgcct atttgaaagt cctgaaagtc ttcaaaatta
    20281 gccatggtga catccacata agtgactttt gcccatttgg atggtccttt acgaacttct
    20341 tgaataaatg tggctatttt atctgaatct tttgattggg ccaggatttc aacggtccca
    20401 tcgttattat tccagacacg accataaata tctcctatat ctagagctaa agtatgggta
    20461 gcgtatcgaa aaccaacacc ttgtacacgt ccagaaacaa ttaaacgtac tttttgcata
    20521 agaagggcct ccttgctttt attatagcac atacttagca ggaaatctct ttagctaatt
    20581 gtctacttcc tgtcactttt actaaagaca agaatacaaa aatgagatag ttagtagtac
    20641 taacagtaaa agaggtttgt ctattttaaa aatagtatca aaaaaaggtc ttttaggttt
    20701 aaaacattga actgccccca aattgaccat ctaagaagtt gatgctttca aatgctgtcg
    20761 cgtatggtat aataggtgca tggttattac atcaaaaaca aatcaactaa ttaagcaaac
    20821 aaaaaaatta ttgcaaaaaa agcatcgaaa acagtcctat ttgattgagg gctggcacct
    20881 ttttgaagaa gcccaaaaat caggacaagt ttttcgccac atctttgttt tagaggaaat
    20941 ggtggagcgt ctagcaggtg agcaagaact cgttattgtt agcccacaag tgctcaagga
    21001 gttgaccgac tctcctagtc cacaaggcat tgttgctgag gtggagatac ctaagttagc
    21061 ctttccatca gattacaaag ggaaatattt ggttttagag gacgtccaag acccaggaaa
    21121 tcttgggacc atcattcgta cagcagacgc tgcacggttt gatggggtct ttctttctga
    21181 aaaatcagca gatatttaca accaaaaaac cctacgttcc atgcaaggta gccattttca
    21241 tttaccaatt tggcgaacag atgtctatca gctttgtcga gagctccaag agtataaaac
    21301 tcccatccta gcaacaactc tgtcaaagaa atctgttgac tataaaagcc tcacacatca
    21361 tgaaaggttg gctttggtgt tgggaaatga aggccagggc attagtgctg aaatggcagc
    21421 cctagcagac caactggttc atattacaat gcctggtcag gcggaaagtt tgaatgttgc
    21481 cgtagcagca ggaatcctca ttttcagctt gatttaagct aatatgatat aataaccaag
    21541 tggggtgatt atgttatgca ttatacagaa gataaagaat atatggaaca cgtcggtcat
    21601 ttgatcgctc atcctcgctt tcaaaaattg agtcatattg ttcagcacca acattcaacg
    21661 cgtcttgaac attctatcaa tgtttcttat tccagttata aattagccaa gcgatttggt
    21721 tgggatgcta aaagtacagc cagagggggc ttacttcatg atttctttta ttatgattgg
    21781 cgcgtgacca agtttaataa gggtcatgcc tgggttcatc ctcgtattgc tgtacgaaat
    21841 gcgaaaaagt taacagaact caataaaaaa gaagaggata tcattctaaa acacatgtgg
    21901 ggagcgacca ttgcttttcc gcgttacaag gaaagttata ttgtgacaat ggtagataaa
    21961 tactgggctg tcaaggaagc agtgacacct ttaagacaaa aatggtctaa tcgacggttt
    22021 cttcgccgca agaccctcca aagtcataat cgttaagtaa aggagttatt atgaacgatc
    22081 acgttattta tacacaatca gatgtgggat taaatcagtt ttttgctaaa atttatagtc
    22141 tagtgggaat gggagtgggt ttatctgctt ttgtttctta tttgatgctt tatccattta
    22201 gagaaaattt aatctctatt cttgttaatc agccgatgat ttactatggt gctgcgatta
    22261 ttgaacttat tttagttttt gtagccagtg gtgcagcacg aaaaaacaca ccagctgcat
    22321 tgcccatttt tcttatctat tctgcgctaa atggctttac cctaagcttt attattgtgg
    22381 catacgctca aacgacagtt tttcaagcat ttctttcttc agcagctgtg ttttttgcca
    22441 tgtcaatcat tggtgtcaag actaaacggg atatgtcagg tcttagaaag gctatgtttg
    22501 ctgctttgat tggtgttgtt gtggctagtc tgattaatct ttttattggt agtgggatga
    22561 tgagttatgt cattagtgtg atttccgtgc taattttctc aggtctcatc gcatcagata
    22621 atcaaatgat taagcgcgtt tatcaagcaa caaatggcca ggttggtgat ggctgggctg
    22681 ttgctatggc cttaagcttg tatttagatt ttattaactt atttatcagc cttcttcgta
    22741 tttttggaag aaatgattaa cattaaaaag tgagtataac tactcacttt ttaatgttaa
    22801 caggataagt aaactgtaag gaagaaattc gagatagata taagatgagg ctgctaaaag
    22861 acctagtgtt aatttgtaaa ctctttcttt ttttggtata ctattaagga ttaaatacga
    22921 ggagaatata gatagatatg tcaacagcta tttggatttt attacttatt gttgcccttg
    22981 gggtaggtgt ttttggtggg atttttattg cccgtaaaca aattgaaaaa gaaattggtg
    23041 aacacccacg tttaacacca gaagctatcc gtgaaatgat gagccagatg gggcaaaaac
    23101 caagtgaagc aaaaatccaa caaacttatc gtaatatcat caaacaatca aaagcagccg
    23161 tatccaaagg taaaaaataa aagctaattg ttatagggga aacctttgtg atagttagaa
    23221 gtgaacagtc tttgatctca atgttcagta gcacttgtct tatcagttag atttgtcaac
    23281 tgttcagggt ttaaaaagaa actcagttgg ctatttccaa ctgagttttt catatcgtca
    23341 gtaaggaagt aaacataatg gatacaagac caattgggtt tttagattct ggtgtgggag
    23401 gtctgaccgt cgtttgtgaa ttgattcgtc agctccctca tgagaagatt gtatatattg
    23461 gtgattcagc aagagccccc tatggtccta gacccaaaaa acaaattaag gagtatactt
    23521 gggagctagt caattttttg ctgactcaaa atgttaaaat gattgttttt gcatgcaata
    23581 ccgcaacagc ggtggcttgg gaagaagtaa aagcagcttt agatattcct gttttagggg
    23641 ttgtcttacc gggggcaagc gcagctatta aatcaacgac aaaaggccag gttggggtca
    23701 tcggaacccc aatgacagtg gcttcagaca tttatcgcaa aaaaatccag ctattagcac
    23761 catctgttca agtaaggagt cttgcttgcc cgaagtttgt accgattgtg gaatcaaatg
    23821 agatgtgttc gagtatagct aaaaaaatag tttatgacag tcta
//
LOCUS       DAUVUY010000018        23262 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.018, whole genome shotgun sequence.
ACCESSION   DAUVUY010000018 DAUVUY010000000
VERSION     DAUVUY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23262
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.018"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..588
                     /gene="pepO"
                     /locus_tag="VOX58_000672"
     CDS             <1..588
                     /gene="pepO"
                     /locus_tag="VOX58_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HES5877923.1"
                     /translation="VNAYYDPQQNQIVFPAAILQEPFYSLDQSSSANYGGIGAVIAHE
                     ISHAFDTNGASFDEHGSLNDWWTQEDYAAFKERTDKIVAQFDGLESHGAKVNGKLTVS
                     ENVADLGGVACALEAAQSEEDFSARDFFINFATIWRMKAREEYMQMLASIDVHAPGEL
                     RTNVTLTNFDAFHETFDIKEGDAMWRAPKDRVIIW"
     gene            complement(754..1794)
                     /locus_tag="VOX58_000673"
     CDS             complement(754..1794)
                     /locus_tag="VOX58_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HES5877924.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(1928..2695)
                     /gene="rpsB"
                     /locus_tag="VOX58_000674"
     CDS             complement(1928..2695)
                     /gene="rpsB"
                     /locus_tag="VOX58_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HES5877925.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADIQADSIEEIVEVVEGDNA"
     gene            2999..6073
                     /locus_tag="VOX58_000675"
     CDS             2999..6073
                     /locus_tag="VOX58_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HES5877926.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLKKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYINSQLGSQPVTEEEMEHLYQASQGNPFFLSEYTQTLLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDRIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKNIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVAY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLQIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADYLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(6104..7090)
                     /locus_tag="VOX58_000676"
     CDS             complement(6104..7090)
                     /locus_tag="VOX58_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HES5877927.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPN
                     LAVLVLQHIAASGKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHN"
     gene            complement(7276..8817)
                     /gene="hutH"
                     /locus_tag="VOX58_000677"
     CDS             complement(7276..8817)
                     /gene="hutH"
                     /locus_tag="VOX58_000677"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HES5877928.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGVACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKRVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLIEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KPENHELGKGTKVAYDLFRKEINFIEHDKHIEIYDELNKASAVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(8828..10171)
                     /locus_tag="VOX58_000678"
     CDS             complement(8828..10171)
                     /locus_tag="VOX58_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HES5877929.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRAPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFENLAKLSVVFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPKEQI"
     gene            complement(10391..10987)
                     /locus_tag="VOX58_000679"
     CDS             complement(10391..10987)
                     /locus_tag="VOX58_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HES5877930.1"
                     /translation="MTKVTIKAPSDYLQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     KSTVALRQTTFTLLDGYHRILMTLDKPITLSDLSHAKEISLTPFEPYAFEGDTRIVSR
                     GICQDINLMYDDQYQGQLLPVWSDTPFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCPNQTSPAQTPVAVWAGLWYKT"
     gene            complement(11009..12682)
                     /locus_tag="VOX58_000680"
     CDS             complement(11009..12682)
                     /locus_tag="VOX58_000680"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HES5877931.1"
                     /translation="MVLSDIEIANSVTMEPISKVANQLGIDEEALCLYGKYKAKIDAR
                     QLVALKDKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKANLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVALAEQDNQFCFVYEEDDSIETKLT
                     KIVTKVYGGKGIRLTPAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFT
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(12700..13326)
                     /locus_tag="VOX58_000681"
     CDS             complement(12700..13326)
                     /locus_tag="VOX58_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HES5877932.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQAIITEIHAKSTALQASLLAAIDKDTEAFNLVSAVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     SAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(13337..14236)
                     /gene="ftcD"
                     /locus_tag="VOX58_000682"
     CDS             complement(13337..14236)
                     /gene="ftcD"
                     /locus_tag="VOX58_000682"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HES5877933.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELDIPIFLYEDSATRPERQNLAKIRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEVIGLAPAKALIDV
                     AEYYLQVEDFDYPKQVLENHLLG"
     gene            complement(14324..16354)
                     /locus_tag="VOX58_000683"
     CDS             complement(14324..16354)
                     /locus_tag="VOX58_000683"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HES5877934.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRWRPKDRIYGKPINEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFQSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIETRHSQGWVTQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTVALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            16646..17911
                     /locus_tag="VOX58_000684"
     CDS             16646..17911
                     /locus_tag="VOX58_000684"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HES5877935.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(17969..19501)
                     /gene="ahpF"
                     /locus_tag="VOX58_000685"
     CDS             complement(17969..19501)
                     /gene="ahpF"
                     /locus_tag="VOX58_000685"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HES5877936.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHGSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     AKTNNMTIIKNVATKDIVGEDHVTGLSYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(19522..20082)
                     /gene="ahpC"
                     /locus_tag="VOX58_000686"
     CDS             complement(19522..20082)
                     /gene="ahpC"
                     /locus_tag="VOX58_000686"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HES5877937.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            20489..20559
                     /locus_tag="VOX58_000687"
     tRNA            20489..20559
                     /locus_tag="VOX58_000687"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20521..20523,aa:Cys,seq:gca)
     gene            21078..21281
                     /locus_tag="VOX58_000688"
     CDS             21078..21281
                     /locus_tag="VOX58_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HES5877938.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            21477..21938
                     /locus_tag="VOX58_000689"
     CDS             21477..21938
                     /locus_tag="VOX58_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HES5877939.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            21938..>23262
                     /locus_tag="VOX58_000690"
     CDS             21938..>23262
                     /locus_tag="VOX58_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HES5877940.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSA"
BASE COUNT         6647 a         4708 c         5125 g         6782 t
ORIGIN      
        1 gtcaatgctt attatgatcc tcagcaaaat cagattgtct ttcctgcggc aatcttacaa
       61 gagccatttt attctttaga tcagagttct tcagccaatt atggaggaat tggtgcagtc
      121 attgcgcatg agatttcaca tgcctttgac accaatggtg cttcctttga tgaacatggt
      181 agcctaaatg attggtggac acaagaagac tatgctgcct ttaaagagcg tacagataag
      241 attgtggcgc agtttgacgg cttagagtca catggtgcta aggtcaatgg taaattgaca
      301 gtttcagaaa acgtcgctga tctaggtggg gtagcttgtg ctttagaagc ggcacaatca
      361 gaagaagact tctcagcccg tgacttcttt attaactttg cgacgatttg gcgtatgaag
      421 gcccgtgagg aatacatgca aatgctagct agcattgatg ttcatgcccc gggtgaactg
      481 cgaacaaatg tcaccttaac caactttgat gctttccatg agacctttga tattaaagaa
      541 ggcgatgcta tgtggagagc tccgaaagac cgtgtgatta tttggtaatt aggtagttga
      601 taaaccttca aatgcctgtg attataaaaa tgactgattc tgtaaaacac aggatcagtt
      661 ttttgcttcc ttattggtgt ccgttttgac acaacaaaaa gacaaccctc aggaaagaag
      721 gttgcctttt ttagacttat cagaaataag tgattagttg ttaagggcag cagccatagt
      781 agctgcaact tctgattcaa agtcgttagc ttttttctcg ataccttcac caacttcaaa
      841 acgtgcaaaa gcaatcgctt tagcgtttac tgagtcaagg taagcttcaa cagttttgct
      901 gtcgtccatg atgtaaactt gtgcaagaag agtgtaagct tggtcaactt tagtgttgtc
      961 aagcatgaag cggtccattt tacctgggat gattttgtcc cagatttttt ctggtttgcc
     1021 ttcagcagca agttcagctt tgatgtctgc ttcagcagca gtaatcacat cgtctgataa
     1081 ttgagctttt gaaccgtatt tcaagaatgg aagagctggt ttgtcaacca ttgcacgaga
     1141 ttcgttgtca agttcgatag cgtggttcaa ttgagcaagt tcatctttga tgaattgtgc
     1201 atcaagctca gtgtatgaaa gaacagttgg tttcattgca gcgatatgca ttgatacttg
     1261 tttagcaaga gcatcgtcgc cgccttcgac aactgagata accccgatac ggccaccatt
     1321 atgttggtaa gcaccgaagt gttgctcatc tgttttttca atcaaagcaa aacgacggaa
     1381 tgagattttt tctccgatgg tagcagttgc attaacgtaa gcttcagcaa gagtttcacc
     1441 tgaaggcata acaagtgcaa gtgcttcatc gttgttagct ggtttgcctt cagcgataac
     1501 tttagcagtt gcgtttacca attcaacgaa ttgagcattt ttagcaacaa agtctgtttc
     1561 agcgttaact tcaacaacag cagcaacgtt accatgaacg taaacgcctg ttaaaccttc
     1621 agcggcaaca cggtcagctt ttttagctgc tttagccatt cctttttcac gaagaagttc
     1681 aacggctttg tccatgtcac catctgtttc aacaagtgct tttttagcgt ccataacgcc
     1741 agcaccagat ttttcacgta attcttttac aagcttagct gtaatttctg ccatgtgtag
     1801 tatcctccaa atgatatttg ttttggtttc aaaaaacgag acagagcggt ttgctttctg
     1861 ccccgtttgg ttttcgattg ttaaagacaa acaaagtttg ccataagagt taggaactct
     1921 gtgctaatta agcgttgtcg ccttcgacaa cttcaacgat ttcttcgatt gaatctgctt
     1981 gaatgtcagc ttcaaaagca acgtctgcat cttcaccttg acggccttcg ataatagcgt
     2041 cagctaattt agcggtgatt aatttaacgg cgcggatagc gtcatcgtta gctggaatga
     2101 tgatatcgat atcatctgga tcagcgtttg tatcaaccat cgctacgact gggataccaa
     2161 gttttttagc ttctttaaca gcaatttgtt ctttatgtgg gtcaacaaca tacatcacgt
     2221 ctgggatacg aggcatatct tcgataccgc ccaagaattt ttcaagacga gcgcgttgtt
     2281 tgttaagaag tgcaacttct ttcttaggaa gaacgtcaaa ggttccttct tcttccatac
     2341 gtttgatttc tttcaaacga gcgatacgtt tttggatagt tccccagttg gtaagcgttc
     2401 cacccaacca acggtggttg atgaagtatt ggcctgcacg cgttgcttcg tcagcaactg
     2461 cttcagcagc ttgtttctta gtaccaacga acaagatgac agcatcgttt gctgcagcat
     2521 cacgaacaaa ttcgtaagct tggtcagcta attttacagt ttgttgaaga tcgataacgt
     2581 ggataccgtt acgttctgtg aagatgtatt tagccatctt agggttccag cgacgagttt
     2641 ggtgaccaaa gtgaacacca gcctcaagaa gttgtttcat tgaaattact gccatgagta
     2701 gtttctcctt tataaaatgt tttttcctct tccagacttc gacttgcagg ctaacctagc
     2761 ggcaacaagc ccacaattgg tctagaatga gtatttgccg cctacacgac cctactatat
     2821 tatcaaaaaa agaggttaaa agcaaggaga aatgctatgg attcccttat ttttgagatg
     2881 actagcttct ttgtttattg gtaagtgtta agacaagctt ttgctcattt tgggtgacac
     2941 ttgccaaatc gttttattta agatatagtg aacttgacta atttttggag gcgttttcat
     3001 gacgaagggt attcgatttc aattgcttgg cagtccccac atttttcttg atgacaaaga
     3061 acaatttttt gcctttgcca aagcaaatgc tctcctttat tacttagtgg ttaatggatc
     3121 tgttagccgc gaggtggcgg cgagtctact ttgggaaaat aagaacacgc aaacagcgaa
     3181 aaagaattta cgaaacgcta tctaccaggt caataaagtc ttgcaagctg atgtgattat
     3241 ttgtcccaat cgcaacctgc tggttttgaa aaaaacactt gatatcaaaa cagatattaa
     3301 cctttttctg gctgatccgc tagctcactt agaactctac caaggggagt ttttgcaggg
     3361 cttttacctt aaaagtggtg aggaatttga tctttgggtt tcaaaaatgc gtatgcagta
     3421 tgaacaggta tacctaaaag cgtgttacca aaaaattgaa gaaaagctct ctttagatgc
     3481 cattgaagat gtcgaggaac atttaaagca gcttattgaa cgtgatgaat ttgaagagaa
     3541 aaattatcag ctcttgatga ggctttatca gcaggggaat tgtcctggta aggtcatcga
     3601 aacttattat cagttggcta atgtgttgga taaggagcta gggattcagc caagtttgca
     3661 gagccaacag atttatcagg aagtggtcgc taaagatcgt aatgaacgca aaatcaagca
     3721 ttttttgcgc aacagtaatc attttttggg gcggattgat gagattaagc agctggaaaa
     3781 ctattttgcc aactgtttgg cctgccaaga ggtaggagct ctgctcttaa ttggtgatac
     3841 agggattggg aaacgaaccc tagccagaca ggtcttagcc aatcaaaccc aaacgtttca
     3901 aattgtcaca gctaaatgtt ttcgtgaaga ggctatggat tccttattgc cttggcgtaa
     3961 tatcttagat ggcctgggag atttggtgat tcaaaaccgc ttattgacca ccaaagcttg
     4021 gaaggctgct cttaaacgct gttttcctgt ggcaaccatt tttcaagaag ataacaacca
     4081 accctttatc aaggaccaca ccagcttgtt ggtttccttt attgttgata ttttacaaca
     4141 tttggcagaa atcaaggcgc tggtaatcct tattgaggat tgtcactgga tggatgagga
     4201 tagtttgacc ttgttgcaac gggtcatgaa tcaattggtc cactatccga ttgcttttgt
     4261 cttgaccaag catctaggaa cgactcctga gctcggtctt tgtttgaatg ctttgatgag
     4321 tcaggggcgt ttagaaagca tttgtttgga gccttttaat cggcaagaaa gtttggcata
     4381 tattaacagt caactgggta gtcagccagt gacggaagaa gaaatggagc acctctatca
     4441 agccagccaa ggcaaccctt tctttttgtc agaatacact cagaccctac tgcgtcacga
     4501 aaaatttgtg cctctaacgc ctgccattaa ggctaagttg ggtcttaaac tagctaacct
     4561 aagtagtcgt gatgacgcat tgttaaacta tttgtcttgt tgtcggaggc ctatccctct
     4621 aaataccctc gctcaattga tgttactgcc tttagaagaa gtgatcgaga tggtggataa
     4681 tctggggcac tactatattt tggtggaaga aagcgtagga gaggaagtgt tgatttcatt
     4741 tcgtcagcga attattcaac tctatagtta tgaccgttta tctttgtcaa aaaggcgttt
     4801 attgcacgga caaattgcca aacggttaga agatttactg cctattttga caccaagccc
     4861 tcacttatta gatgacattg cttaccatta tcaggaatca cggcaggtga ttaaggcttt
     4921 ggaatacaac cttaactatc tagatgctac cttgccattt cagcatgagc tgtttcccat
     4981 ttattcaaag agtattggct ccttggaaaa gtcagatcgt gaccatcaac gcttaatgga
     5041 agagcagttt gataggattc gtcaaagcat tgcggatttg gaactgacct atgataataa
     5101 ccgtgatttc cagcagttgc ttattcgttt ttcttacctt gaaggccgtt atgatattcg
     5161 aacagggagg tatcaagaag ggattaaaaa cattcaaaag gtcatcgctt tggcaaccga
     5221 gctaaaacag ccctccttct tgctagaagg ctatcgccag ttaattcatt actgtatcca
     5281 agtggaaaac aagccagaaa tgcgttatta cactagtctt tcattggagg cagcggttgc
     5341 tgctaatcat tttgaggcca ttgcgattag tttgcggtta aacggtcttt atcatttgat
     5401 tattggggag ttaaatgagg cagagcgtct gcttcaacag tcgattgact tctttaaggt
     5461 gacgccaggc ttgcaagcta actatgcgat tcagattgct gctgccttag attatttggg
     5521 ggagatcgcc cagattcgct atcagtttga aaaggccgtt gcttaccaaa aacaagccat
     5581 tgccttgacc gaaaacaagc cagctgagtt atcagtgagt attttttata ttggattagg
     5641 gatttcctac ttttatttag ctgattttga acaagctgag caaatcttga gtttggctaa
     5701 ggaagctttg gtcaatcata gttatccttg gaaagaaacg caacttcaaa tttacctggc
     5761 catgattcaa tggaaaaagg gcaattatca gccagccttg accctacttg attacagaga
     5821 aaccttgatg tctaggtatc gcaatccgcg tgataagggc ttggttttct atttgatggc
     5881 tgtggtcaag taccaattga tatttcaagg agctacccta agtcagcagg aaaaagaaat
     5941 ggcagactac ctgttgtcag aatcctttga gtattattat gaaattgcta gtactaacct
     6001 caacccttac cgagattgtc atttggtgag tgagttgaac gatttgcgtc aacaattatc
     6061 tgctaaaagc taaggaaaac cctctgattc acatcagagg gttttagtta tgttgtgcca
     6121 taatctgtac gaggtaaaag ataaaggtgg ctgctaaatt ggcggtgtgg ttatcaatat
     6181 cgtgtggtgg tgaaacctca accacatcaa agccaactaa ttttccgcta gctgcaatgt
     6241 gttgcaaaac taagacggca agatttgggt caacaccgag ggactggata gcactaaccc
     6301 caggtgcagc accaactgag aaacaatcca tgtcgatagt caggtagacc ctttcttgcc
     6361 cttctaagaa acggtcaatg gctcggcaga ccttttgatg gcccatttgg tagatgtctt
     6421 gacctgttaa aaattgaatg cccttggatt ttgccacaaa gtcaaatagg aaaaggttgt
     6481 tattatgttc ttgaatgcct aaaacgaagt atttaaagag gcgtttgtca gcaactgcat
     6541 cgtcaaacat ttgtcgaaag cctgtccctg aattagggcc tgtttggtcg taaggcctta
     6601 aatcaaagtg ggcgtccata ttgatgacag ctaggtcatc acttggggat aggctttggc
     6661 gtaggcctaa gtagtggccg taggcggttt catggccacc tcctaagacg atgggtttga
     6721 gattgaggtc acacatccgt ttgatggctt tgcttaaact gttttgcagt tgttccaaag
     6781 agcgattggg gccgtcaatg ttacccacat cgtaaaccat aacttggttt cctaaatgcc
     6841 aagggaattt ggctagctgg gttcgtatgg cagctggact ttcgaccgct ccgacacgac
     6901 cattattgat ataaacgccc ttatcacttt taaagccaat caaggcaaaa tgcgtccctt
     6961 caaatggggt tagtgagctg tcgttaaggt caaggaaagt catgaccatg ccccatttgg
     7021 cagtatataa gtcatcgtcg atcccaccat gataatagct agtggtggat gggtagtaat
     7081 cttctagcat gcgcgtcatc tcctttagga ttatgatagc aaaataaaac ttacagtcaa
     7141 cactttcttg gcctttgtca ctggttttta ggaatagcag agcaataaaa tcgtctgctt
     7201 ttggtcccag ttagttagtc taaagaggcc atgacctctg ccacagcccc tctagttaaa
     7261 gaagtacctt attcactaaa attggatact caactcaact gcctgctcaa ccgcttcgag
     7321 gaagcttggg tcttcaatga ctgctgatgc cttgtttagt tcatcgtaga tttcgatgtg
     7381 tttatcgtgt tcgataaagt tgatttcctt gcggaagaga tcgtaggcaa ccttagtccc
     7441 tttacctagt tcatgatttt caggtttgag atcaagagct tggcaggctg ccatgatttc
     7501 agtagccacg atgcggcgag aatttttaag gatttcaaag gccttgcgag cagcggtagt
     7561 gcccatgctg acaaagtctt cttggttttc acaagatggg atagaatcca cgctggctgg
     7621 atgtgccaaa accttgtttt cagaagcaag agaggcacaa gcgtactggg taatcataaa
     7681 gcctgaattg agacctgggt atttgacaag aaaggatggc aatttgctga gctggctatt
     7741 gaccaaacgt tccacgcggc gttcagaaac atttccaatt tcagaaatgg cgatgcctaa
     7801 gaagtcaaat ggctgtgcca ttggttctcc gtggaaatta cctcctgaaa taacgtggcc
     7861 atctttacag ataattggat tatctgtgac agaattgatt tcaatgtcaa ctttggattt
     7921 gacataggcg atactgtctt tgctagcccc atgaatttga ggcatgcagc gcaaggtgta
     7981 agggtcttgc acacgagatt gagtagctac ggttgtgttt tggctgcctt caatgaggtt
     8041 tcggatattg cgggctgttg ccaactggcc gctttgtggg cgaatggtgt gaaggttttc
     8101 ttcaaatgga ctagtaatcc cgttatgcac ttcaagggat agggcaccag ctaggtctga
     8161 cagtttcaat aattgaatgg cgtcataggt tgcaagggca ccgattgcag tgagaacagt
     8221 tgttccgttg ataagggcga gtccttcttt agcggctaaa gaaatcttgt caataccagc
     8281 tttatcaaga gcttcttgac cagaaagaag ctctcctttg tagtaagctt tccctagacc
     8341 aagcatagga agcaccatgt gagcaagcgg cgccaaatct cctgaagccc ctagagaccc
     8401 tttttcagga atgtaaggat gaaccctttt gttgagcagt tccaacagct tttcaatggt
     8461 ggatagacga ataccagaat accctttgac gagagagttg atacggatca gcatgatagc
     8521 tctcacggca tcttctggca atgggtcacc aaatccacta gcatgggtac gaatcaaatt
     8581 ttcttggagt tggactgtat cttctggtga gatgcttacg ttacaaaggg atccaaaacc
     8641 agtggtcacc ccgtaaacca cccgtttttc actgacaatg tcatcaacaa ttttacgaga
     8701 ggcattcaca gcttcaatag cactatcatc aatgcgacag gctacgcctt ggcgagcaat
     8761 cgcaatcacg tcttcgatag taaggctttc gccatctaaa ttaatcactc ttgtcatagt
     8821 ttttctccta gatttgttcc ttagggggca tttggtccat ttggtggtta cgtccgtgca
     8881 taaggtagta cacactactt gcaatgatga caccaactgc accataaaca aagttcattg
     8941 ggttgtcacc ccaaatcata actaaactaa cgataactgc aagaattggg ataataggac
     9001 caaatggtac tcggaagatc acattagcat cgggatcgtc cttccgtaat ttcataacgg
     9061 ctagtgctgt tggaatgtat tggaagaagc ggaaaacaac actgagtttt gctaagtttt
     9121 caaatgagcc agtaagtagg agtacaatgg caattgctcc tgaaaccaag atagcaacga
     9181 gtggtgcgcc attttgattt tgcttggcaa tagcagctgg taagagccct tcatcagcaa
     9241 tggcagcacc gtaacgtggc accatgatgg attctcccat gttaagacct gtaatggaaa
     9301 ttaaagcacc gattgagacc atccaagcgc cagcagggcc tatcatttta acaaaggcat
     9361 cttgaacagg tgcattggtc atcatgattt gagagccaag catggcaatg gttccaccga
     9421 taatcagcat atataagaca gagacgatac tgattgatcc taaaagagca cgcggtacat
     9481 ttttttcagg ggcacgcatt tccccagcga caatagagag ggtttcaaaa ccgataaagc
     9541 catagaagat ataaacagca gtattggaga tagcccctaa gaggttagtc cctggttcca
     9601 actgaacaaa tggggtaaag ttaggaagcc cgtttttgat gaaaaaaagg gtacaggcac
     9661 aaaaggcaac gattggaatt aacttagcaa tggtggctgt gatggtgaca attttagagg
     9721 ttttcaaacc agcgatattc atcaaagata gtaagatgat taacccaata ctaagtggaa
     9781 tgtgccaacc ttcaaaggca gggaaagtaa tgataaacat tctggcaaac ccagctgcca
     9841 tggctgccca agcgaagata gtaacggtcc aaccaaggaa gccaacgtta aagccaataa
     9901 aatcaccaaa agctcgtttg gaatattgga aggctccgcc gtttttccca aaataaccag
     9961 agacttctgc aaaacaaact gccaacatga tggtcaaaat agctgttcca aacataacag
    10021 cgatggatgc agggccaagg cccttgtaaa tggccctagg taagagaaaa ataccagagc
    10081 cgataacggc attgatcccg taaagagtgg caccgctcaa gctaaacttt gcctgttctc
    10141 tttcttgttc gttcatgtga tttgacgcca taataatgct ctcctttttg tttgcaaatt
    10201 gcgttcaaat ataatggata aggtcgctag gaaatgagga gatagtcggt ttaggagggt
    10261 ccttcttgca cctaacgata gtgatgagct attggttagc gatgagaaat aggtagcctt
    10321 ttgtgatgat gtgtagtgat ttcattggag aagaacaggc atgagtagtg tgatgcctat
    10381 ctttatccca ctaagtctta taccataagc ctgcccaaac agctactgga gtttgggcgg
    10441 ggcttgtttg attggggcaa acttttagat ctgaaaggct ggcctcctgc tcgattacca
    10501 acaagtgatt ggcttcaagc tcttgattcg cctgattggt cacagaataa gtcagtttgc
    10561 ttagggcata aagcagttga cagtccgctt tgggtgcctc aaatggtgtg tcagaccaga
    10621 cgggtaataa ttgcccttgg tattggtcat catacattaa attgatgtct tggcagattc
    10681 cacgactgac gattctggta tcgccttcaa acgcataggg ttcaaaagga gttagggaaa
    10741 tctctttagc atgactcaaa tcactcaatg tgatgggctt gtctagggtc attagaatcc
    10801 tgtgatagcc atcaagtaag gtaaaggttg tctgtcgtag ggccacagtt gactttgaga
    10861 ggcgataaga gaattctctc tttttgtaat ctccatcttc gggataaagg aacaattggt
    10921 tggtttcacc gccagaccag tcagtttgga ggtaatcaga gggcgctttg atagttacct
    10981 ttgtcatttg ttctctcctt gtttccaatt agaatagtcc tgtgatgttg ccctcctcgt
    11041 caatatcaat tgtttcgctg gcaggtactt ttggaaggcc aggcatggtc atgatagcac
    11101 ccgtcagagc aacgataaag cctgctcctg cggacacttt gaggttgcta atagtcactg
    11161 taaagtcagt tggtgcgcca agttttttgg catcgtctga gaaggaatat tgggttttgg
    11221 ccatgcaaat tgggtagttg ccaaagccga ggcgttctaa gtcagccagt tcacgtttgg
    11281 ctgcgggggt caatctaatc cccttaccac cgtaaacctt ggtaacgatt ttagtcagtt
    11341 tggtttcaat gctatcatct tcctcataaa caaagcagaa ttgattgtct tgctcggcta
    11401 gtgccacgac tttttcagct aattcacggc caccagctcc gccatttgcc caaacatcag
    11461 aaatcacaac gtcaacgcca cgtttgtcgc aggcatcata gacagcttgt aattcagcat
    11521 cggtatcaag cgggaattta ttgatcgcca cgacaactgg aagaccatag acgtcttgga
    11581 tattagccaa atgtttgtca aggttaggta aaccatccac aacggcctga acattttcag
    11641 tagccaggtt tgcttttggc acacccccat gcatcttaag ggcacgtata gtggctacta
    11701 ggacaacggc tgctgggcga aggcctgaca tgcgacattt gatgtcaatg aatttttcag
    11761 ctccaaggtc agcgccaaat ccagcttcgg taacggcata atcgccatat ttcaaggcta
    11821 gtttggtggc taggacacta ttacagccat gggcgatatt agcaaatggg ccaccgtgaa
    11881 tgagggctgg tgtgtgttcc agggtttgca cgagattagg atgaatcgca tccttgagca
    11941 gagcggctag ggcaccacca gctttcaagt ctttagctgt tacaggttca ccttggtagt
    12001 tgtaaccgat gatgattttt tcaaggcggg ctttgaggtc agaaatattt tcggataggc
    12061 acaggattgc catgatttca gaggcgacgg taatgtcata accatcttca cgaggaaccc
    12121 cgttgacctt gccttgcaag ccgtcaacaa tatgacgaag ctggcggtca ttcatatcca
    12181 ccacacgttt ccaagtgata cgtctagaat caatgcctag gctattgcca tggtgaatgt
    12241 ggttatcaat caaggcagcc aataagttgt tggcgacacc gatggcatga aagtcccctg
    12301 taaagtgaag gttgatgtct tccattggga caacttgcgc gtggccaccg ccagcagctc
    12361 ctcccttaac cccaaaaaca ggaccaagag aaggttcacg aagggcaata actgcttttt
    12421 ttccaatggc agaaagagca tctaccaatc caacggaagt agtggttttg ccttcaccag
    12481 ctggtgttgg tgagatagca gtgacaagaa tgagtttgcc atcaggtttg tcttttaagg
    12541 cgaccagttg acgggcatca atttttgcct tgtacttgcc atagagacag agcgcttcct
    12601 cgtcaatccc taattgattg gcaaccttac tgataggctc catagtgaca gagttggcta
    12661 tttcaatatc tgataaaacc atagttgctc ctttacttgt taaacaatat cgagaatttt
    12721 cgtgtagatg tcatctgcca agtgacagcc cttgtctaat aaggcttgtc ctttttggcg
    12781 atagtcggta acaaagtctt catctttgat gccagacaga ttaatcaaaa cattcaacca
    12841 agcgctttgt aaacctgctt ttaagtttaa agcagcaact cctaagtcgc tagcagcatt
    12901 tgtattagac ttaccgacag ctgttgcagt gatctcaagt gcttcgacca tgagggtcat
    12961 catctcaaaa ggtgattgtg cagctgtttt caaggccttt tgcatggcag ttcgtctagc
    13021 agccttgtct tcgtcagtct cttttggcat atcaaaaacg gcagaaacca ggttgaaggc
    13081 ttcggtatct ttgtcaatgg ctgcaagcaa gctagcttgt agagcggtgc ttttagcatg
    13141 gatctctgta ataatagctt gataatcagc atactttttc ttaccaaggg tcaattcaca
    13201 gaccatcttg gtcaaggaaa tgccattagc gccagagaga gcagcagcag aaccaccacc
    13261 cggagcaggg gcatctgagc caaggacttt agcgaaatct gttaagctta aatcaactaa
    13321 acccatagag aacctcctaa cctagcaaat ggttttcaag aacctgttta gggtagtcaa
    13381 agtcctcaac ttgtaagtag tattcagcca catcgattaa ggccttggct ggcgctaagc
    13441 cgatgacttc agaaccaata acattcacac cgtagcgacg cgcttcaaat ttgatggttt
    13501 caaaagtacg gtaaagagaa catttttcaa agttaaccat gttcatggag acttgggcaa
    13561 tatggcggtc ttctaacatg acaccgatag ccttacaata tttgtagcca ccacctgaac
    13621 cgcggataat tttagcaatt ttatgagcaa tgtcgatatt gtcagtatcc aaattaacat
    13681 tgaaagcaac caatggcatg cgagcaccaa cggcagttac cccagcagtt gggtgaatct
    13741 tacgatctcc gtagtcaggt gcccaatcct cttccaatag tttttctggc atgccttcaa
    13801 actgaccttt acggatttta gcaaggtttt gacgttctgg acgtgtggca gagtcttcat
    13861 aaaggaagat cggaatgtca agttcacggt taatgcgttc tgcgacttgt ttggaaattt
    13921 cgacacattc ttgtgtagtg atgtctttga ttgggacgaa cgggcagaca tcggttgctc
    13981 ccatacgagg atgttcacca tgatgcttgg tcatgtcaat gttttcagaa gcgtatttca
    14041 ccagttggaa agctgcttct tgaatagact ggtcatctcc aactaaggta aacacgctcc
    14101 ggttgtggct cgcatcagaa gagtagtcga gaagggtcac tccaggaata cttttggccg
    14161 tcgctaccaa accatcgata acagcctgat tttggccttc tgagaagttg ggaatacatt
    14221 caacaatttt tgccatgtta atctcctttt atgtcattga gttactcatt aggggaatag
    14281 gttacctact ttagataacg cttacatttt tagctagcag ttattagtga aataattgat
    14341 ctaccgctga agtcacaagg tcatcatcag ccaagtatgg gatagtgatg tgatcagtac
    14401 ctgcatggag acggttgtat tcaatagcgg tttcaatagc gtgctcattt cgggcccagt
    14461 tacggcgagc tacaccgccc atagtatccc aagcaatagc agatttaata atctcatcaa
    14521 tgcgttcgct gccatctaaa acaagtccga aaccaccatt gatggcttta ccaataccag
    14581 ttccgccacc gttgtgaaga gccactaaac tcataccacg cgcagcgtta ccagcgtaac
    14641 attgcaccgc catatcacag gtcacattgg aaccgtcttt aatgtttgag gtttcacgaa
    14701 atggtgagtc ggtaccagac acgtcatggt ggtcacgacc aatcatgaca ggtccaatct
    14761 tgcctttgcg gaccaattca ttaaatttaa gggcgacagt cacacgaccg atacagtctt
    14821 ggtaaaggat ccgggcttgg gtaccaacta cgagttgatt tttctcagca tcacgaatcc
    14881 agttgtagtt atcacggtct tgatagcggc ggtcaggatc gatagcttcc atggcagctt
    14941 tgtcagtagc caccaagtct tcgtgtttac cactcaagca gacccaacgg aaaggtccat
    15001 agccatagtc aaaaagcata ggtcccatga tatcttccac ataggatggc cagataaatc
    15061 cgtctttgtc gttacgacca ttcttagaaa tttctgtaat accagagtcg taaacagact
    15121 tcataaaggc attgccgtag tcaaagaaat aggtgccatt ttcagttaac gtcttaatag
    15181 cttcaaaatg ccgtgccaag gtatcatcaa ccatttggtg gaaggtatct ttatcttctg
    15241 ccaagagccg ggttctttcg tcaaagctaa tacctactgg gcagtagcca ccgtcataaa
    15301 cgttgtgaca agaggtttgg tcagacaaaa gatcaacatg aatctgcttg tcattgacgt
    15361 actctaaaag atcaacgata ttaccatggt aagcaatcga agtggactct ttagcatcta
    15421 tggctttttg ggctaattgc agagcttctt cagggctctc agcaatttgg gtaacccagc
    15481 cttgcgaatg acgggtttca atccgagatt ggtcaacttc agcaataatg gcaactgctt
    15541 tagcaatttc agcagcctta ccttgtgccc cactcatgcc tcctaaacca gatgagataa
    15601 agagtttacc ggtcaaatca ccatcgtcag ccaccccaag tttgagacga ccagcattta
    15661 agagggtatt aaatgttcca tgaacgattc cttgtgggcc aatatacatc caaccacctg
    15721 cggtcatttg accatagttg gtgacaccca tctcttcagc gatttcccag tctttcatgt
    15781 tgtcgtattc acccaccaaa aggccattag taataatcac acgaggcgct tcaggtttgg
    15841 attggaaaag accaactgga tgaccagact caaccaccaa ggtttgttca tcagtcatga
    15901 cttctaggta ttttttaatc aaacaatatt gcatccagtt ggcacaaaca gaaccagttt
    15961 ctccataagt gactagttcg taagggtaca aggcaatctc aaaactcaag ttattgtcaa
    16021 tcatgacctg catggccttg gcagctgtac aatttccctt gtactcgtta atgggtttgc
    16081 cgtaaatgcg gtcttttgga cgccatctgt aaccatagat gcgtcccctt gttttgagtt
    16141 cttccaaaaa ttctggaatg acttcttcgt ggaacttcgt aggtacatag cgtagggcat
    16201 ttttcaaggc gatttcggtc tgagcctggg tcaatcgaaa acctctgtct ggtgctcttc
    16261 ggataccttc tttgaagaca gtcttttccg gtaaaacatc gtctaactta acagtcatag
    16321 cggcagctat gtcagtttca ctgtaaaatg acatagaaaa ctcctcctca actccttttt
    16381 attgtcgaga ttagtctaac ttagactagt ctataaaaag tcaaccacgc tttgaaatgc
    16441 gaaaaaagtc taaaaaaaaa tcaaaacctc attttttgac taaaatttga cgcttaatca
    16501 gtacacttga ggcatgacag ataaagtcag tcagagttta attgttgtta ccaaagttgc
    16561 agagatcgca actattgagt gggtgagggg ggactagtaa tggctattaa tcagttgaca
    16621 aacgatgtta aaaaaggagg tttttatggt tgctgatgtc ttattaacac attttaatca
    16681 actattttgt ctaaatgatc caggtcatcc tttaactggt caagaaatga aaaaagccac
    16741 tatcgttgaa gatggctata tagccatcaa agacggcctc attgtggctc ttggttctgg
    16801 tgaaccggat gctgagcttg tcggtccaca gactattatg cgttcttata aaggtaaaat
    16861 tgcaacgcct ggtattattg actgtcatac ccatttggtc tatggtggca gtcgggaaca
    16921 tgaatttgcc aagaaattag caggggtgtc ttacttggat attttagctc aaggtggtgg
    16981 tattttaagc acggtgaggg ccactcggtc agccagcttt gacaaccttt atcaaaaatc
    17041 caagagattg ctagactaca tgttgcttca tggagtgact actgtcgaag cgaaaagtgg
    17101 ctacggcctt gattgggaaa cagaaaaacg ccaactggat gttgttgctg cgttagaaaa
    17161 agaccatcct attgatttgg tgtctacgtt tatggcagcc catgcgattc ctgaagaata
    17221 caaagggaat cctaaggctt atctcgatgt gattatcaag gatatgcttc ctgttgttaa
    17281 agaggaaaat ctggcagaat tttgtgatat tttctgtgaa aaaaatgttt ttacagctga
    17341 tgagtcccgc tatctgttaa gcaaagccaa agaaatgggc ttcaagcttc gtattcatgc
    17401 agatgaaatt gcgtctatcg gcggagttga tgtcgcagca gagctcagcg cagttagtgc
    17461 agagcatttg atgatgataa cagatgatgg tatcgctaag ttgattggtg ctggtgttat
    17521 tggtaatttg cttccagcca ccacctttag tttaatggaa gacacttatg cccctgctcg
    17581 caaaatgatc gatgccggca tggctatcac tctctcaaca gatagtaatc caggcagttg
    17641 cccgactgct aacatgcagt ttgtgatgca attaggttgt tttatgttac gtttaacacc
    17701 gattgaagtc ttaaatgctg ttaccattaa tgcggcctac tctgtcaatc gtcaggagag
    17761 ggttggtagt ttgacagttg gtaaagaagc agatattgcc atctttgatg ccccaaatat
    17821 tgattaccca ttttatttct ttgccaccaa cttgattcac caagtctata aaaaaggaca
    17881 actcacggtt gatcgaggtc gtattcttta aaaaatttca aaaagaagct ttgagcgtta
    17941 gctcaaaact tcttttaaat atgctatttt attgacgaat caagtagtca aaggcaccga
    18001 tggcagcagt agcaccagac cccatggaaa taatgatttg tttataagct gaatcagtac
    18061 agtctccagc agcaaagata ccaggaatat tggttgaccc atgtttatcc acaatgattt
    18121 caccgcggtc ggttaggttg acaccactat ctttgagcca agctgtgttt ggaacaagac
    18181 caatttgaac aaagacccct tcaaggtcaa ggtgtttgtc ttcaccgcta tcacgctcag
    18241 tgtagctaag acctgtaaca tggtcctctc caacaatatc cttagtagca acatttttga
    18301 tgatggtcat attgttagtc ttagctgcgc ggtcttgaag cactttgtca gctttaagtt
    18361 caggcaagaa ttctaaaacg taaacgtgtt tagcaagacc agccaagtca agggccgctt
    18421 ctaatccaga gttcccacca ccgataacag caacatcttt gccttcaaag agaggaccgt
    18481 cacagtgagg gcagtaagtc acccctttat tacggaattc atcttcacca ggaacattaa
    18541 tgttacgcca tttggcccca agagctaaga tagcagtttt ggcttgtaga accgcaccat
    18601 tagctagagt aacctcaatg ttttcttttt tctcaataga agtagctagt tgtgctttga
    18661 taatatcaac atcataagac ttagtatggg cctccacttc ggccattaat tttggtcctt
    18721 cagtgtaaag ggtaccaatc atgttttcga tgccgaccgt ttccatgacc tgaccaccaa
    18781 aggtttcagc aagtaaacct gtttttagtc ctttacgagc tgcatagata gcagcgctat
    18841 tgcccgcagg gccaccacca ataacaagga catcatagag gcctttatca gcaaaggctt
    18901 cctcagaaag tgggccagcg atttgttcta agagttgctc aatggtagcg cgaccagaag
    18961 tgaattcttc cccgtctaaa aagacagtag ggacagacat aatgcctttt gctttcactt
    19021 catcttggaa catgccgcct tctaccatag tgtgtgaaat cttgtcgttc aagacggaca
    19081 taatgtttaa agcttgaaca acatctgggc agttatggca ggttaagctg acataagttt
    19141 caaaatgcaa cggacggtca atggccttga tgcgatcaat aacatcttga tccacttttg
    19201 gagcacgtcc agatacttgc aagagagcaa gaataaagga cgtcaattcg tggcctaaag
    19261 gaagaccagc aaagacaacc ccactaccgt gacctttttt cgcaacctta aagcttggtt
    19321 gtcggtctaa agtgatgttt tcaatagaaa tacgctcaga catagcagct atttcttcca
    19381 caaagtcttt gactttttga gactgctcat tgtcaccaag ggaaacttgc aaaacaagat
    19441 cagcttctaa cagggtgagg tattgggcaa gttgttcttt aatatcagga cttaatgcca
    19501 tagctttcct cctaaaattt attaaatttt accaactaaa tcaagacttg gtgtcaaagt
    19561 ttcagcgcct tctttccatt tagctggaca aacttcacct ggatgtttac ggacgtattg
    19621 ggcagcgtga attttatcaa tcaaggtgct agcgtcacgt ccaataccat cagcattaat
    19681 ttccatcatt tggataatac catctgggtc aacgataaat gttccacgtt gagcaagtcc
    19741 gtcttcgcca agcacttcaa aggcttgtga aataaggtgt gaagggtcac caatcatagg
    19801 gtatgtgata gtgccaacca catctgagtc atcatgccaa gctttatgaa caaaatgagt
    19861 atcagtagag acagaataaa cttctacacc aagagatttc agtgtttcgt attgctcttg
    19921 aaggtcaccg agttcagttg ggcaaacaaa tgaaaagtct gctgggtaga aacaaaaaac
    19981 tgcccatttt cctttaacgt cttcatttgt aacagtgatg aattttccat cgtgataagc
    20041 ttgagctgaa aattcagcaa tttcttttcc aattagagac atatagttgt cctccttttt
    20101 tatagtttta tacttgtatt ataaaagaac aacaagatta tgtctactca gaaacccttg
    20161 attttactgg gaaatcagcg attgttttct gacaattcag ttgtgaaata aatcacatta
    20221 attaaaatga ttctaaataa gctgaagacg ccgttaaatc aaaggttaat ttaattataa
    20281 tctacagaaa atgtaattgt tattaaagcg tttacaaaaa aacttttttg attccgttaa
    20341 ggcaacttaa tattatggtt acctaatcgc taatattgta acctattttg gcatgagatc
    20401 agggaatggc tgttatatta attggatatg tcttgtgcaa atagtgcttg acggatttgt
    20461 ggaattaggg taaaataatc tttgttgtgg cggtatagcc aagtggtaag gcacggctct
    20521 gcaaaagctt gatcgtcggt tcaaatccgt ctaccgcctt ttatatacaa aacaagcctt
    20581 gattaatttc gtgaaaacgt tgttaaatca aggtttttat tttttgcgac aggataaaaa
    20641 aagagaaaaa aagaaataaa gtcggtcaaa agtcggtcaa atttcccgaa gatgtctttt
    20701 atagcctgat tattaggtat gaaaaatact atggccaaaa ttggtaaacg attgcaaata
    20761 aaggtcagag ttcttacccc cttaaacgat atgcgttaaa agttagttga actgccgtgc
    20821 acctaacgat agacacggtg atgtgagagc gagtagagtt gaatctctac ttgtttatct
    20881 taccatagtc agctttataa agtaaccacc cattacaatg caactatatt cttaatgttt
    20941 ttaagcatgt tattgacaag tgttataaaa tgtgttactc ttagtgagta aattagtttt
    21001 tgagttagta tttgtgtttc aacatcttta tttctctcta tttttacaaa tgatttttag
    21061 aaaggaagtg gatgtatatg gcacaaggta cagtaaaatg gtttaacgct gaaaaaggtt
    21121 ttggttttat ttcaactgaa aacggtcaag atgtctttgc acatttttca gccatccaaa
    21181 ctaatggttt taaaacgtta gaagaaggac aaaaagtggc atttgacgtc gaagaaggtc
    21241 aacgtggacc tcaagcagtc aatatcacta aattggccta atctggttaa agtaactaaa
    21301 gagaagcaga tgttggaaac aacaactgct tttttgattt ctaccagtta cctttaatga
    21361 ttaagagttg cgtttaaggt cttgtatttg ttataataat agtcagtttt agtcaaaatg
    21421 tattgacgtg gtgctactaa gtttttagag tagcccgcaa gcaaaggagt gacagcatgc
    21481 caaccaaaaa tacttcagac agcattgaag agtatattaa agaattatta gctaagtcag
    21541 gaatagcaga aattaagcgg tccatgctag cagattcgtt tcaagttgtc ccaagtcaga
    21601 tcaactatgt cattaaaacc cgttttacgg agagtcgagg ctatgaagta gagagtaaac
    21661 gaggtggtgg aggctatatt cgtattgcca aggttcattt ttcagacaaa caccatctga
    21721 ttggcaattt aatggcaact attgaagact gtatcagtga gcaagttttc acggactcga
    21781 tacaattgct ttttgacgaa cacttattga cagagcgtga gggaaatatc attttggctg
    21841 tggcgtctga tgatgtttta ggaactgatg gttcaactat tcgtgcacga atgctctatc
    21901 gcttattgca acgaattgac agaaagggaa gcaattaatg attatgtatt caacgaagat
    21961 gcaagacatt tttagacagg cgcagttcca agctgctcgc tttgatagcc attgcctgga
    22021 aacttggcat gttttgttag ctatggtagc tgtagataat tctttagcaa atatgatttt
    22081 aagtgaatat gatgcccaag tcgccataga agaatatgaa gctgcagcta ttttagccat
    22141 gggcaaaacc cctaaggaac agttgtctcg tgtagacttc agacctcaat ctaaaacctt
    22201 gactaacttg ttagcttttg cgcaggctat tagccaaatc actagggatc aagaagtcgg
    22261 ctctgagcat gtcttatttg ctattttatt gaatccagat atcatggcga gtcgtttgtt
    22321 agaaatagca ggctatcaga taaaagataa cggcaatggg cagccgcgat tagctgactt
    22381 gcgaaaagca atagaacgtc atgcaggtta cagtaaggaa atgattaagg ctattcacga
    22441 actacgtaag cctaaaaaaa cgaaaacaca agggaccttt tcagatatga tgaagccacc
    22501 aagtacagct ggtgaattga gtgatttcac aagagatttg actgaaatgg caagacaagg
    22561 tttgttagaa tcggtgattg gacgtgacca agaagtatct cgtatgattc aggtactaag
    22621 tcgtaaaacg aaaaacaatc ctgtcttggt aggtgatgca ggtgttggta aaactgcgct
    22681 tgcttatggc cttgctcaac ggattgcaaa tggcgctatt ccttatgaac ttaaggagat
    22741 gcgtgtccta gaattagaca tgatgagtgt ggtagcagga acccgttttc gtggggattt
    22801 tgaagagcgc atgaatcaaa tcattgatga tattgaagct gatggtcaga ttattctttt
    22861 tgttgatgaa ctacatacta ttatgggttc tggcagtggt attgacagta cacttgatgc
    22921 ggctaacatt ttaaaaccag cattatcgcg cggtactctt catatggttg gagcaacaac
    22981 tcaagaagaa tatcaaaaac atattgaaaa agatgcagct ctttcgcgtc gttttgctaa
    23041 aatattaatt gaagaaccta atacagaaga tgcttatcag attttgatgg gcctaaaatt
    23101 atcttatgag acctaccata atgtctcgat atcaaatgag gcagttaaaa cagctgtaaa
    23161 aatggcacac cgttatttaa ccagtaaaaa tctccctgat tcagctatcg atttattaga
    23221 tgaagctagt gctgctgtgc aaaacatggt gaaaaaatca gc
//
LOCUS       DAUVUY010000019        23221 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.019, whole genome shotgun sequence.
ACCESSION   DAUVUY010000019 DAUVUY010000000
VERSION     DAUVUY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23221
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.019"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..361
                     /locus_tag="VOX58_000691"
     CDS             <1..361
                     /locus_tag="VOX58_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HES5877941.1"
                     /translation="CFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYV
                     LETSSVMLQVSYFKYTKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLW
                     GVGSLASLLVLAILYVF"
     gene            519..1862
                     /locus_tag="VOX58_000692"
     CDS             519..1862
                     /locus_tag="VOX58_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HES5877942.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHFQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERNAKKQHF"
     gene            2057..2860
                     /locus_tag="VOX58_000693"
     CDS             2057..2860
                     /locus_tag="VOX58_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HES5877943.1"
                     /translation="MTSVFLTSGWAFYDYLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKFLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            2860..3603
                     /locus_tag="VOX58_000694"
     CDS             2860..3603
                     /locus_tag="VOX58_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HES5877944.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYSRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            3707..3931
                     /locus_tag="VOX58_000695"
     CDS             3707..3931
                     /locus_tag="VOX58_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HES5877945.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            3995..4912
                     /gene="trxB"
                     /locus_tag="VOX58_000696"
     CDS             3995..4912
                     /gene="trxB"
                     /locus_tag="VOX58_000696"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HES5877946.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDQEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            5081..6460
                     /locus_tag="VOX58_000697"
     CDS             5081..6460
                     /locus_tag="VOX58_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HES5877947.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            6631..8085
                     /locus_tag="VOX58_000698"
     CDS             6631..8085
                     /locus_tag="VOX58_000698"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HES5877948.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPGNFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAMIGGADATSNVRAGKRFDIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKTYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMTLIDNIRKDTHGKSE"
     gene            8087..8911
                     /gene="nadE"
                     /locus_tag="VOX58_000699"
     CDS             8087..8911
                     /gene="nadE"
                     /locus_tag="VOX58_000699"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HES5877949.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKAAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            9096..10433
                     /gene="pepC"
                     /locus_tag="VOX58_000700"
     CDS             9096..10433
                     /gene="pepC"
                     /locus_tag="VOX58_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HES5877950.1"
                     /translation="MSALTETFTEQLFAHYEANAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQC"
     gene            complement(10897..13062)
                     /locus_tag="VOX58_000701"
     CDS             complement(10897..13062)
                     /locus_tag="VOX58_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HES5877951.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDISNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(13049..13657)
                     /gene="recU"
                     /locus_tag="VOX58_000702"
     CDS             complement(13049..13657)
                     /gene="recU"
                     /locus_tag="VOX58_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HES5877952.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            13738..14253
                     /locus_tag="VOX58_000703"
     CDS             13738..14253
                     /locus_tag="VOX58_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HES5877953.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQEVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
     gene            14375..14701
                     /gene="gpsB"
                     /locus_tag="VOX58_000704"
     CDS             14375..14701
                     /gene="gpsB"
                     /locus_tag="VOX58_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HES5877954.1"
                     /translation="MTSIIYSPKDIFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNA
                     QIEALKAENEALKKAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVF
                     GKQIIE"
     gene            14715..15083
                     /gene="rnpB"
                     /locus_tag="VOX58_000705"
     ncRNA           14715..15083
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VOX58_000705"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            15146..16300
                     /locus_tag="VOX58_000706"
     CDS             15146..16300
                     /locus_tag="VOX58_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HES5877955.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWGNYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            16314..17783
                     /locus_tag="VOX58_000707"
     CDS             16314..17783
                     /locus_tag="VOX58_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877956.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERGIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAAQAQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGYQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(17929..18411)
                     /locus_tag="VOX58_000708"
     CDS             complement(17929..18411)
                     /locus_tag="VOX58_000708"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HES5877957.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(18485..19816)
                     /locus_tag="VOX58_000709"
     CDS             complement(18485..19816)
                     /locus_tag="VOX58_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HES5877958.1"
                     /translation="MEIIGSLGVLIGVIVIIYLSVKEVNIIIAAPLATSLVILFNQMD
                     PTTTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGETTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMARG
                     ETYATYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIMIAFDLFS"
     gene            complement(19887..20666)
                     /locus_tag="VOX58_000710"
     CDS             complement(19887..20666)
                     /locus_tag="VOX58_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HES5877959.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETFLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(20699..21358)
                     /locus_tag="VOX58_000711"
     CDS             complement(20699..21358)
                     /locus_tag="VOX58_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HES5877960.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(21360..22010)
                     /locus_tag="VOX58_000712"
     CDS             complement(21360..22010)
                     /locus_tag="VOX58_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HES5877961.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDTLVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITVDGKDYLLEKPLKADIALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREIVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(22034..23221)
                     /locus_tag="VOX58_000713"
     CDS             complement(22034..23221)
                     /locus_tag="VOX58_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HES5877962.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
BASE COUNT         7146 a         4174 c         4845 g         7056 t
ORIGIN      
        1 ctgctttaat cacaaacctg ctaaagtatt tatgggagat gtaggtagtt tagcccttgg
       61 agctatgttg gctgccattt ctattgcgct tcgtcaagaa tggactctcc tgattattgg
      121 gatcgtttat gttcttgaaa caagttctgt gatgttgcaa gtgtcctatt tcaagtacac
      181 caagaaaaaa tatggagaag gtcgtcgtat ttttagaatg acgcctttcc accaccattt
      241 ggaattggga ggcttgtctg gtaaaggcaa gaagtggtca gaatggcagg tagatgcttt
      301 tttgtgggga gtaggaagtc tagctagttt actagtttta gctatcttat acgtgttcta
      361 agggggatta tcaagaatag tcaccaatag cattttgatt aacatcatca ataagagagt
      421 ggagatgacc cacaaatgct gattttgtga gtgttgtccc actccttttg cttgtctcta
      481 acaataatga tataatatgg aagaaaacga ggtaaaacat gtcatttaaa gattatcatt
      541 ttaagcaata cgttcagcaa gcgctggaag aaattggctt tgtcaatcca acagaggtcc
      601 agaagcggct gattcctatt gttaactccg gtcgtgactt agttggggaa tcaaaaacag
      661 gttcagggaa aacccacacc tttttgttgc ccatttttga aaaacttgat gaagctaaag
      721 cagaagtgca agtcgttatt acagcaccta gccgtgaatt agcaactcaa atttttgatg
      781 cttgtaaaca gattgctaag cacttccaag aagagattcg tttggccaac tatgttggag
      841 gcacagacaa actccgtcag attgaaaaac taaaaggcag ccagccgcat attgtcatcg
      901 gaaccccagg tcgtatttat gacttggtca agtctggtga tttagcgatt cataaagcaa
      961 cgacctttgt tgtggatgag gccgatatga ccatggacat gggctttttg gatacggtag
     1021 ataagattgc agcttcgctt ccaaaatctg ttcagatttt ggtgttctca gcaactattc
     1081 cacagaaatt acaacccttt ttgaaaaaat acttgaccaa tcctgttatt gaacaaatca
     1141 agacaaagac cgttatcgca gataccattg ataactggct tgtgtcgaca aaagggcgtg
     1201 ataagaatgg tcaactgtta gagattctca aaaccatgca gccttatatg gctatgcttt
     1261 ttgtcaatac caaggaacgc gcagatgatt tgcatgcctt tttaacagct aatggcttaa
     1321 aagtggctaa gattcatggg ggcattcctc caagagaacg taagcgcatc atgaaccaag
     1381 tcaaaaaact ggactttgaa tacattgttg ccacagactt agcagcgcgt gggattgaca
     1441 ttgaaggggt cagccatgtg atcaatgacg ccatcccaca agacttgtct ttctttgtcc
     1501 atcgtgtagg ccgaacaggt cgaaatggta tggcaggaac tgccattaca ctttaccagc
     1561 caagtgacga ctcagatatc aaagagctgg aaaaaatggg gattgccttt acaccgaagg
     1621 tgctcaaaaa tggagaattt caagatacct atgaccgtga tcgccgtcaa aaccgtgaaa
     1681 aagcctatca aaaattagac acagaaatga ttggtttggt caaaaagaaa aagaaaaaag
     1741 tcaaaccagg ttacaagaaa aaaattcagt gggcggttga tgaaaaacgt cgcaaagaac
     1801 gtcgtgctga aaatcgtgct aagggccgcg ctgagcgtaa cgccaaaaaa caacattttt
     1861 aagaacaaag agcaagcaag tgatcaattg gcatctaatc ttgctttttt tacacctgct
     1921 acttgtattg tttaccattt gttaacacaa aaagtgatca tagcttttgc ttattgctat
     1981 aatacggatt tactatttta catgtgggtc taagagtgtt agaatattaa gacaaacaaa
     2041 tacaaagggg aaatacatga catcagtgtt tttaaccagt ggttgggctt tttatgatta
     2101 cctcatctca ccgattccac atggaaaact ttttagttgg catgctgttt ttgatgccat
     2161 tccaaatatt atccaacggc ttccaattac gcttggtttg acactatcag gagcaacctt
     2221 tggcttggtt ttggctttga tttttgccct tgtcaaaatt aataaagtga agctactata
     2281 ccctattcaa gctatttttg tgagtttctt gcgaggaact cctattttgg tacagttgat
     2341 gttgacctac tacggtattc ccctctttct aaaatttctc aatcagaagt atggctttga
     2401 ttggaatgtt aatgcaattc cggcttctat ttttgccatt acagcctttg cttttaatga
     2461 agcagcctac gctagtgaaa ctattcgggc agccatttta tcagtagata caggtgaaat
     2521 tgaagcagca aaaagtctag gcatgacttc tgtgcaggtt taccgtcgtg tcattattcc
     2581 taatgcaact gttgtggcta ttccaacctt gattaatggg ttaattggct taactaaagg
     2641 aacatcgctt gcctttaatg cagggattgt cgaaatgttt gcccaggctc aaattctagg
     2701 cggatcagat taccgttatt ttgaacgtta tatctctgtt gctcttgtct attggtctat
     2761 cagtattttg atggagcaag taggtcgctt gattgaaaac aagatggcca tcaaagcacc
     2821 agaacaagct agaaatgaaa agttaggaga attgcgttga tgattacaat tagaaatttg
     2881 agcaagacat tctcagggca aaaagtttta gatagtttgg ctcttgatat tgaaaaaggt
     2941 caagtgattg ctttagttgg tgcttcagga gcagggaaat cgaccttcct tcgtagccta
     3001 aactatttgg aaaaaccaga ttcaggatcc attagcattg gtgactttac ggttgatttt
     3061 gagaccatta ccacagagca agtccttatt ttacgtcgga aacttgccat ggtttttcaa
     3121 caatttaatc tttttgagcg tcgtacagcc cttgagaatg tcaaagaagg tcttaaggtg
     3181 gtcaaaaaac tgtctgacca agaagcgact aagctggcgc aagcagaatt ggctaaggtt
     3241 ggtttagcag atcgaaaaca tcattactca cgtcatttat cagggggaca aaaacaacgg
     3301 gtagctttag caagagctct ggctatgaaa ccagatgttt tattactaga tgagccgacc
     3361 tcggctcttg acccggaatt agttggcgaa gtggaaaagt ctattactga tgctgctaaa
     3421 tccggacaga ccatggtttt agtgagtcat gacatgaatt ttgtttacca agtggcagat
     3481 agagtactct ttttagatca agggaaaatc ttggaacaag gaacacctga agaagtcttt
     3541 agacatcctc aaaaagaacg aaccaaagaa ttttttgcaa gctattcaaa aacatacatt
     3601 taatgatatt cttttgttat aataaaaagg tctaggtatt cctgggcttt tcttgttatg
     3661 ttttttagaa aaacttatgg gaaagaaata atgactaggt ggaaatatgt tagtagaaat
     3721 ctttagcctt tatgtaaaag gtctcattct ctcaagcctt actatgattt taatttgttt
     3781 aatatggact ttatggcgag caaaaaccaa aagagataaa acattgattg aaagacaagc
     3841 ttttctttat gagatgctaa tggttgctat tttaaccatc ccaattttat cgtttgcttt
     3901 tatgagtatt ttggttgtca taaaatccta atcattgcaa gaagggatta tcattgctaa
     3961 aatattggca agagtagatt aaaggagaaa aatcatgtac gatacattaa ttattggttc
     4021 aggaccagca ggaatgactg ccgctttata cgctgctaga agtaatttat ctgttgccat
     4081 tattgaacaa ggggcacctg gtggtcagat gaacaacact tttgatatag agaattaccc
     4141 gggatatgat catatatctg gaccagaatt agctatgaaa atgtacgaac ctttagagaa
     4201 gtttaacgtt gaaaacattt atggtattgt tcaaaaaata gaaaattttg gtgactataa
     4261 gtgtgtgttg actgaagatg ctagttatga agctaaaacg gttattattg caacgggagc
     4321 taaataccgt gtactaggtg ttccaggtga agaatactat actagtcgag gagtttctta
     4381 ttgtgcagta tgtgatggtg cgtttttccg tgaccaggat cttttggtcg ttggtggtgg
     4441 tgactcagca gttgaagaag ctatttatct tactcagttt gctaaaaagg taaccgttgt
     4501 tcatcgccgt gatcaactaa gagctcaaaa aatactgcaa gatcgtgctt ttgccaatga
     4561 taaggtcgac tttatctggg attcagtcgt caaagaaatt caaggcaatg atattaaggt
     4621 ctccaatgtc ctaatcgaaa atgtgaaaac aggccaagtc actgaccacg cttttggagg
     4681 tgtgtttatc tatgttggta tgaatcctgt gacaggtatg gtaaaggatc tagaaattac
     4741 cgatcaagaa ggttggatta tcacagatga tcatatgagg acaagtattc ctggtatttt
     4801 tgcgattgga gatgttcgtc aaaaagacct tcgccaaatc acgacagctg ttggagatgg
     4861 tgcaattgca gggcaaggtg tttatcacta ccttgaaagt ttcccttcat aaggttaaaa
     4921 ctccttcatt tttccttgtc tttcccttaa aaaagctaaa ttgcataaga cctttaagaa
     4981 atataaagaa cagttttcct taatatcttt cctagataaa tgttgaaaat tttctgaaaa
     5041 aaggatataa taaatagtat tttagtgaat ggagtttaga atgtctataa aagaacaaac
     5101 agacaataac gaactagaaa atggaatggt aaggggctta gaaaatcgtc atgtgcagtt
     5161 aattgcgatt gcaggaacga ttggaacagg tctcttttta ggggcaggac gctctattgc
     5221 cctaacagga ccatctatta tttttgtgta tatgattaca ggtgccttca tgtttatgat
     5281 gatgcgtgcc atcggtgaaa tgctctatta tgatccagat caacacacct ttatcaattt
     5341 tatttccaaa tacattggtc caggctgggg ttatttttcg ggtctatcct attggatttc
     5401 ccttattttt attggaatgg cagaaatcac agcagtaggt gcttatgtgc aattttggtt
     5461 cccaagctgg ccagcctggt tgattcagtt ggttttctta gtcttactta gttcgatcaa
     5521 cttaattgct gtgcgcgttt ttggggaaac agagttttgg tttgccatga ttaagatttt
     5581 agctatttta gctttgattg caacagctat tttcatggta ttgacaggtt ttgaaactca
     5641 cacaggccat gctagccttt ccaatatttt tgaccatttt tccatgttcc caaatgggaa
     5701 actaaagttc tttatggcct tccaaatggt tttctttgct tatcaagcta tcgaatttgt
     5761 gggaattacc acttctgaga cggctaaccc aagaaaagtt ctaccaaagg ctattcaaga
     5821 aattccaacc cgtattgtga tcttttatgt gggagccttg gtctctatta tggcaattgt
     5881 gccatggcat cagttaccag ttgatgaatc tccttttgtg atggtgttca aattgattgg
     5941 tattaaatgg gcagcagcct tgattaactt tgtggtcttg acatcagcag cgtcagcgct
     6001 taactcaacc ctttattcaa ccggtcgtca tctctatcag attgctaatg agactccaaa
     6061 cgctttgacc aatcgcttaa agattaatac tttatctcga caaggggtgc caagtcgtgc
     6121 cattattgct tcagcggttg tggttggtat ctcagctttg attaatatcc taccaggagt
     6181 tgcagatgcg ttctcactca ttacggcctc ctcatcaggt gtatatattg ctatctacgc
     6241 cttaaccatg attgctcact ggaagtaccg tcaatctaag gactttatgg cagatggtta
     6301 tttgatgcca aaatataaag tgacaacgcc tttgaccctt gctttctttg cttttgtctt
     6361 tatctctctt ttcttacaag aatccaccta tatcggcgcc attggagcaa cgatttggat
     6421 tattgttttt gggatttata gcaatgtcaa atttaaataa taattagcga tatagaactg
     6481 aatccattaa ctctttctta tttgaggatg tcatgatgga tcagttcttt ttttacttta
     6541 gcgataactc aagttatttc taaaccctag ccaaagagtc ctttttaaaa tatgttataa
     6601 tagtaacaat aattcaaagg agtccccttt atgtataaag atgatagttt aactctccat
     6661 actgatttgt atcagattaa tatgatgcaa gtttattttg agcagggcat tcataatcgt
     6721 catgcggtgt ttgaagtcta tttccgcaag gaacctttta acaatggtta tgctgttttt
     6781 gccggtttac agcgcatggt ggagtatcta gagcaatttc aattttcaga aacagatttg
     6841 gcttaccttg aagaactggg ttatccagga aactttttga cctaccttaa agagttacga
     6901 ttagaattga ccatccgttc agctaaagaa ggagatttgg tttttgctaa tgagccgatt
     6961 gtgcaagtag aaggccctct aggtcagtgt cagttggtcg aaacagctct tttaaacatt
     7021 gttaacttcc agaccttgat tgctacaaag gctgctcgta ttcgttctgt gattgaggat
     7081 gaaccccttt tggaatttgg tacccgtcgt gcacaggaac tagatgctgc gatttgggga
     7141 acccgcgcag cgatgattgg tggagcagat gcaacaagta atgtgagagc aggtaaacgc
     7201 tttgatatcc ctgtttcagg tacgcatgct catgctttag tgcaagccta tggtaatgat
     7261 tatgatgctt tcatggctta tgctaagacc cataaagatt gtgtatttct tgttgacacc
     7321 tatgacacac ttaaagtagg tgtgccaaca gctatccgag tagccaaaga aatgggtgac
     7381 aagattaatt ttctaggtgt tcgattggac tctggtgact tggcttacct ctcaaaaaca
     7441 gtccgtcagc agttagacga cgctggtttt accgaggcta agatctatgc ttctaatgac
     7501 ctcgatgaaa ataccatcct caacttaaaa atgcaaaagg ccaaaattga tgtttggggt
     7561 gttgggacca aactgatcac agcctatgat cagcctgcct taggtgctgt ttataagatt
     7621 gtctctattg agcaagaaga tggtagcatg cgtgatacga tcaaattatc aaataatgca
     7681 gaaaaagtat ccacaccagg taaaaaacaa gtttggcgca tcaccagtcg tgaaaaaggc
     7741 aaatcagaag gcgactatat cacgtttact gatattaatg ttaatgaatt aactgagatt
     7801 gagatgttcc atccgaccta tacctatatt aagaaaaccg tcagaaattt tgatgcaatt
     7861 cctctcttag ttgatatttt tgtcaaggga gaattagttt accagctacc gaccttatct
     7921 gaaatcaaga cttatgctaa aaaagaattt gacaagcttt gggatgaata caagcgtgtc
     7981 ttaaatcctc aagactaccc agtagacttg gcacgagatg tgtggcaaaa taagatgacc
     8041 ttgattgata acattcgtaa agatactcat ggaaagagtg aatgacatga cattacaaga
     8101 agagatcatt cgacaattag gcgtcaaggc tagcattgat ccacaagaag aaattcgaaa
     8161 aaccgttgat tttttaaaag cttatttgcg aaagcactca tttttaaaaa cttatgtctt
     8221 aggcatttct ggaggtcagg attctaccct cgcaggaaag ctagcccaga tggccattgc
     8281 agagttacgg gaggaaacaa gtgatcaagc ttaccaattt atagctgttc gcttgcctta
     8341 cggggttcag gctgatgaag cagatgcgca aaaagccttg gcttttattg caccagatca
     8401 gacccttacc attaatatca aggcagctgt ggacggtcaa gtagaggctc tacaagcagc
     8461 aggcgttgag atttctgatt ttaacaaagg taatattaaa gcacgtcagc gaatgataag
     8521 tcaatatgcc attgctggcc aaatggctgg tgctgttatt ggaacggatc atgcggcaga
     8581 aaacatcact ggtttcttta ctaaatttgg tgatggtgga gcagatattc ttcccctctt
     8641 taggcttaac aaacgtcaag gaaaggctct tttgaaagtt ttaggagcag atgctgcttt
     8701 gtatgaaaaa gttccaactg ctgatttaga agatcaaaaa ccaggcttgg ctgatgaggt
     8761 tgccctaggg gtgacctatc aagacatcga cgactatttg gaagggaagc tgatttccaa
     8821 agcagctcaa gcaactattg aaaaatggtg gcataaagga caacacaagc gtcacctgcc
     8881 aatcaccatt tttgatgatt tttggaaata agaaatgatg ttcaaagagt aaaaaaagaa
     8941 aacaaaaaaa gttaggttat cctaactttt ttcttgaaga aaatcaaata aggtctataa
     9001 tagaagtaat cctttagaat agaatggctg gataataacc atcttgccaa tattatttca
     9061 ggtgaagctt atcttaatat tttaggagga caataatgtc agcattaaca gagacattta
     9121 cagaacaatt atttgctcat tacgaagcca atgcaaaatt ttcagctatt gagaatgccg
     9181 ttacgcataa tggtttgcta aaatcattag aaacccgtca aagtgaagtg gacaatgatt
     9241 ttgtgttttc tattgattta actaaagata aggtatctaa ccaaaaggca tctggtcgct
     9301 gctggatgtt tgctgccttg aacactttcc gacacaaatt aatcacagag tttaaattag
     9361 aaaattttga actgtcacaa gcccatactt tcttttggga taagtatgaa aaagctaact
     9421 ggtttatgga gcaggtcatt gctacagctg accaagaatt aaccagtcgt aaagttaagt
     9481 ttctcttgga tgttccacaa caagatggtg gccagtggga catggtggtt tccttgtttg
     9541 agaagtatgg agtggtgcca aaatctgttt acccagaatc aatctcatca agtaacagtc
     9601 gtgaattaaa ccagtatttg aataaattat tgcgccaaga tgctcaaatt cttcgtgacc
     9661 tgattgcatc aggtgctaag gcagaccaag ttgaggatcg aaaagcagag cttcttcagg
     9721 aaatttttaa cttcttggcc atgacccttg gtttaccacc tcgtcatttt gattttgcct
     9781 accgtgataa agatgaccac tatcatgttg agaaaggttt gacaccgcaa gcgttttacg
     9841 acaaatttgt tggcttaaag ctttctgatt atgtgtctgt tattaatgcg ccaactgctg
     9901 ataagcctta tggcaaatca tacactgttg agatgctagg aaatgtggtc ggtagtcgtg
     9961 aggttcgcta cctcaacctt gatatgaaac gtttcaaaga actagctatc aagcagatgc
    10021 aggctggtga gtccgtttgg tttggctctg atgtgggtca agtttcagat cgtcaaaaag
    10081 ggattttggc aactaatact tacgattttg aagctagtat ggatatcaat cttagtcaag
    10141 ataaggctgg acgtttggat tatagtgaaa gtttgatgac acatgctatg gttttaacag
    10201 gtgttgactt agatgaaaca ggaaaaccgt taaaatggaa ggttgaaaat tcatggggtg
    10261 agaaggttgg tgacaaaggt tactttgttg cttcagatgc ttggatggat gaatatacct
    10321 atcaaattgt tgtccgtaaa gaattcttaa cagctgacga gttagcggct tatgaaaaag
    10381 agccacaagt actagctcct tgggatccaa tgggtgcttt agcacaatgc taattccttt
    10441 ctttgcgact gtatgaaatc taaaggctgg acaaaacgaa cagcaaacgg attggactcc
    10501 gcataagaaa gtgaacatga aaaaaattat tatgctaaga aaatgacata tgaaaaaaca
    10561 cgtcttcggt atatggaaac gtgttttttc atgtttactc tcttatggca ttagttgcta
    10621 tttataacaa ttttaaataa gtctattttg ttttaggtta acgtttgaaa aatcagttgc
    10681 taattccctt aacgttgtac cttttcttta atacgattaa agaattctgt tttttgtttt
    10741 atattgtatg tggtaagtca tttgtgtcat ataaaaacga gtcctcacag aacgtggact
    10801 cgctttttat ttagattatc acttaaggta gatattaaaa gtaactaaat ggatatgtaa
    10861 taaaaggtag gtacagacaa taagtagagt tagagattaa tgagttgtct ttttatcatc
    10921 ggtagatgga tgattgctat tgttgtctgt attggttgaa ttacttatat cattgctact
    10981 actagtatca tctgaagtcg tttggctaga agctgttgtc gtgttattgc tgtaaagatt
    11041 gttgtaaacc gaattagtat agtcggtgtt gctcgcatag gttccgctta agtagaggaa
    11101 tccaccactg cgataaagac catttggcat cgtccaatct tcactgtaac catttgttaa
    11161 gtaagtcatc atgctacgat aaacgtcaga tgcaatctct agacttgatc cgtatactgg
    11221 ggtcaagcgg tttttgtaac ctgtccaaac agccatggcg tagcgcttag taaagccaac
    11281 aaagttttcg tctggcgcta atgtaccaac ataatctgga taaaggccgt atttttcacc
    11341 aattttagct agttcctcat cagtgtagtt agaagtccct gttttaccag cttgcgcaac
    11401 accaggaatg gcagcagcag tacctgtacc ataagtgaga acagttttta acatatctgt
    11461 catcatatag gccgtggttt ctttcatggc acgttttcct ttttcatcaa aagttttact
    11521 tgtaccatca ctaaattcca ctttattgac ataccttggt ttatgataaa taccaccatt
    11581 tgcaaaagca gcgtatgcag cggccatttt ttcactactt gcaccatatt ttttatctga
    11641 gctactgtta ttacttgaga tagcgtttga gtagtgcatt tcgggatagt taatacctaa
    11701 actgcttaag aaagatcgag catagtcaag tcctgcggct tccagtgctc ggactgctgg
    11761 gacatttcgc gatagcataa tagcagcttg gattgtcatc catccgttat atctaaggtc
    11821 ccagttaaac aattgggtag tggttccagg ccaataatag actgagtcat tagtcgactg
    11881 agcagtagaa gtataaacac cagattcaat agcaggagca taggctgtga ttggcttcat
    11941 ggtagaaccc cagtcacgat cagttaaaac agcttggtta gtcccaaatg aaacattttc
    12001 atcttgatta cgtccgccaa gctgagcaat aacatgacca tttgtcacat caacaatcgt
    12061 tgaagcaact tggaaatctt ggtctggata gtaaacataa tcaccagaat gataaatatt
    12121 ataaagagtc tgctgcgcat cggggataat attggtataa acttttaaac cagcggtaaa
    12181 aatatcttta ttcgtttctt ttttgacttc ttcaataact tgttttagat aattatccat
    12241 atattttgga taagttgagc gctgttggag tgattgtaga ccttcagcga cgggagttgc
    12301 gatggcagtt tcatattctg ctttcgtcag atgtttttcc atgtacatct gttgcaacac
    12361 gacgttacgg cgattttgag cagcttcagg atgaaggtaa ggatcatatt gactaggagc
    12421 ttgagggatt ccagccaata gggctagttg ggcataagat aaatccttaa gatccttgcc
    12481 ataataagac ttagcggctg tcagcatacc atagttgcca ttacccatat atactttgtt
    12541 gatgtaaaaa gtcaggattt cttgtttagt atattttcgc tccatttgaa gagcaagcca
    12601 aacttcttga gccttacgtt ttaaggtttg atcagattca ttagtagaaa aataggctag
    12661 tttaatgagt tgctgatcaa gcgttgaccc cccttgagtg gtctgactcg ttagattatg
    12721 aaaggcagca ccaaaaatac gataaagatc tactccacga tggttaaaga aacgtttatc
    12781 ttcaattgag gtaatagcat taactagatt aatagggata ctatcagctg ttacattttc
    12841 acgcttttca gaacccaaat cagcaatcag attgttatta ccgtcataaa ccaagctaga
    12901 gtttgttgat tttaactggg cttctgacag tttcggagca ctgctaatgt agaaggtaaa
    12961 caaaagacca ccaataataa taacaaggat aataaggcta agaattgcac ttaatacata
    13021 ctttagccac ttaaggattt ttggattttt aattgtaatc accgcctaaa aaattttgtt
    13081 caattatgtt taaataaggg acttgtggaa aggctccaaa tgctacctta aagccatttt
    13141 ttctgatata atcaatgggc atcgatttac tgccattatc tatctgataa aatgaaatca
    13201 aggcttgagc aggtaggtag taagtctcct tcagtgtgga aaagtgaaga aggacaaagc
    13261 agattccttt ttgatgtaaa acgcaagcca tatgttcaat ctgatgtaaa tggaaatttt
    13321 tcatgggcat agctgttttt tgcctggttt cttttgcttc aaaatcaaca taatgtcctt
    13381 tataaacgcc gcaatagtca gtcgttgaag cttgtctaaa gtacgcttca acgatttttg
    13441 cccgactgcg tttagggtaa tccactttga caatttggac aggagtaggt tttttgtgaa
    13501 tgacagctat ttgacgagat aaataataat catttgtcgc gttgatagca gcttcaaagg
    13561 acatacctct attggcgaag tccaccttat tttgctttgt ttgcttttga acggaactta
    13621 ctttttgtcg aataagatta tgaggataat taaccattgt tctcctaata ttatctagtt
    13681 aatgctaaaa atttaagcac ttttattata acatattttt tgaaaggata acacctaatg
    13741 actgcgattc taattacagg ttaccgaagc tttgaaatag ggatttttga ccataaagac
    13801 ccacgagttt caattattaa acaggctatt cgtaaagatt taataggtta tttggaaaat
    13861 ggtgtggatt ggtttatctt tacagggaac ttgggttttg aacaatgggc tttggaagta
    13921 gctaatgagc taaaagagga gtatcccttg cagatagcta ctatattctt gtttgaaacg
    13981 catggagata ggtggaatga aaaaaatcaa gaagttttaa gtcagtttag ggctgttgac
    14041 tttgtaaaat attactttcc aaattacgaa caaccgacgc aatttagtca atattatcag
    14101 tttttattag aaaagacaga aggggcttat gttttttatg acacagaaaa cgagaccaac
    14161 ttaaaatatt ttctcaagaa agcaaaagat atgccgcatt atcagttgct attgctaacc
    14221 tttgatcggc taaatgatat gtcacaaagc tagcattgta tttttagggg tattggtatt
    14281 taaatacttg ctttttgaac tatttttctg tatcattaat aataatactg aaatataatt
    14341 ttggtaaaaa gattgaccgt tcggagagta aagaatgaca agtattattt atagtcctaa
    14401 agatattttt gagcaagaat ttaagactag tatgcgaggg tttgataaga aagaagttga
    14461 tgaatttctt gataatgtaa ttaaagatta tgaaaatttc aatgctcaaa ttgaagcttt
    14521 aaaagctgag aacgaagcct taaaaaaggc caagtttcaa gctagaaata cagtttcagc
    14581 gactgttcaa caaccagtac cgcaaccgac tcgtgttgct caatcagcta ctaattttga
    14641 catcttgaaa cgtattagta aattggaaaa agaggttttt ggtaaacaaa ttattgagta
    14701 atgaaaactg tgcaattttt ggataatcgc gtagtatttt aatactatga ggaaagtcca
    14761 tgctagcact ggctgtgatg ccagtagtga ttgtgctagg cgaacacata agcctaggga
    14821 tgtgcataca cattacggcg aaggaaatgg ctaagtcgtt tgatatgcca aagtacttct
    14881 gaaagtgcca cagtgacgta gtttttatgg aaacgtaaaa aatggaacgc ggtaaacccc
    14941 tcaagctagc aacccaaact ttggtagggg catgggatag ttggaaacga acaagctatc
    15001 ctgactgtca acagacggta gacagatgat tatcgaagga aataattcct agttatttcc
    15061 ggaacaaaac atggcttata gaaaattgca taataggtta agctagcact tgctagcttt
    15121 tatttgattt aaaagaaaga gatatatgaa agaaacattt agattagtcg caactgcagc
    15181 cgcaggttta gaagctgtcg ttggtaaaga agtcagagca ctaggttttg attgtcaggt
    15241 tgaaaatggg aaagtttatt ttgaaggtga tgtagaagcg attgttaaaa caaatctttg
    15301 gttacgtgca gcagatcgta tcaaaattat cgtaggacaa tttcctgcta gaacctttga
    15361 agaattattc caaggtgtct ttgccttgga ttggggaaat tatttaccgc taggagctaa
    15421 gtttccaatc tcaaaagcca agtgtgtcaa gtcaaagcta cacaatgagc caagtgttca
    15481 ggcgattacc aaaaaagctg ttgttaagaa actgcaaaaa catttccaca ggccagaagg
    15541 agttcctctt caagaagttg ggtcaacgtt taacattgag gtatctattt taaaagacca
    15601 agccactatt atgattgaca cgacaggatc tagcctattt aaacgagggt accgcgttca
    15661 aaagggtggg gctcctatta aagaaaatat ggcagctgct atcttggcct tatctaactg
    15721 gtttccagat aaacctctgg ttgatcccac ctgtggatcg ggaacgtttt gcatcgaggc
    15781 agccctgatt gggatgaata ttgctccagg atttaaccgt tcgtttgctt ttgaagaatg
    15841 gagctgggta gataaagaca tggtgcaaca agttcgtgat gatgccgaac aagaagctaa
    15901 ttatgaaatc gaattagata tttctggctt tgatatcgat ggccgcatga ttgagattgc
    15961 taaaagtaat gctgaagaag ctggtttatc tgatgtgatt acctttaagc aaatgcgtct
    16021 gcaagacttt cgaacagaca aggttaatgg tgtagtgata tcaaatcctc cttatggaga
    16081 acggttgctt gatgacaaag cggttgacat cttgtataat gaaatgggac aaacctttgc
    16141 accattgaaa acatggagca aatttatcct tacaagtgat gaactttttg aactaaaata
    16201 cgggcaaaaa gctgataaaa aacgtaaact ttataacggc accctaaaag ttgatttgta
    16261 ccaattttat ggggaaaggg taaagcgaca tctgatatag agaggtagta agcgtgtcag
    16321 aagaaagcaa agaagtcgaa gtaacaaaag aatcgcaaac attggggctt aatgaagcca
    16381 agtcaatgac aattggtgaa gctgtacgca aacagtcaga gataaaagca ggtgttacta
    16441 aggatgatag tattttagat aagtacatta agcaacaccg tgacgaagtt tcttctcaaa
    16501 agtttgatgc taagtataca gagcttgata cagctagttt agataacttt atcaaaaaac
    16561 aaagagaagc attgagtaaa gccggtcttg ttgatgacga acctgtgtct gcggagtctg
    16621 ctgagcaaga tagcactttg gttgaagaag tagctgaaga cttagctcca atggagacta
    16681 ctgcggtggt aactgggatt ccggtggaag cgacagtccc tgtgttagat ctagatccat
    16741 cagagagggg cattccagaa ccacagatga ccaaggaaga gccaaaaaga gatcagtttc
    16801 tttctgaaga tagccaccat ccagccaaac aaaacacgaa aaaaggttgg ttaatagctt
    16861 tatttctact attattggcc attcttgcag ttgtatttgg ttggaaccat ttcttgagac
    16921 aagacagtgg taaaacaact caaacagcaa gtaaacagac aaagacctct ttgcagaccg
    16981 acagtgctaa aaaagcaacg cgcttgaagg ctgctgctaa agctttcgaa aaacaatatg
    17041 gtacttttta tacagatgcc acaaagagca aattaaaaaa tagtgccttt gctactttgc
    17101 cagatttaga agctgccttg aaagctttgg aaggctctgc ttactatgat aaggcgaaag
    17161 caaaagtcga ttcccttaag aaagctattg ctgctattac agcggttaac ggtaaatttg
    17221 tttccgatgt tgttgtagat ggtgaaaagg tttcggctga agtcaaggct gatgctaatt
    17281 ttgatgattt atcaagtgca accttgacaa caggaaatgc aaatcttgat gctgtcttgc
    17341 aggcaagcat cacagaaggt cgtcagcaac tagccagcaa ggctgaggcc gctaaagcag
    17401 ctaatgagca agcagcccag gctcaagcag ctcaaggaca aagtacatca gtagcaccat
    17461 ctggctatgg cttgacaagt tatgaccctg caagcttaca acggcattta tcacgtgtac
    17521 cttacaatca agatgttatt gctgacagag ctaacccatc ttgggcattt aatccaggag
    17581 ttcttgaaaa aattgtagca acctcacaag ctagaggtta tatttcaggc taccaatata
    17641 ttttagagcc ggttaatatt attaatggca atggttacta taatatgttt aagcctgatg
    17701 gcacttatct gttctctatt aattgtaaga cgggttattt tgtaggaaat ggcaaaggtt
    17761 atgctgatgc tttagattac tgagaaaatc aaaaaaaact gagataaggc aacttgtctc
    17821 agttttttga ttaataaagt atgttgtctt aaaatctgca gatagacttg ttttttgtaa
    17881 caagtcaaat aggtcagtag gattgttatg tttgacaaca cactagtatc agatgacatg
    17941 gcgactaaaa ggatcgtctg aaatcccttg atcaataatc aattgagccc attctttggc
    18001 ggcaaagagg ctatgatcct tatagtttcc acaggattca agagttgttc caggaacatc
    18061 ttcccaagta atcccagttg caatttcttc taggctggat ttaatcacct tggcaatatc
    18121 agtagaactg tgttttcccc acatgataag gtgaaaacct gttcgacagc caaaaggaga
    18181 gcaatcaatc atcccatcaa tgcgttggcg gatgagcttg gcaagtaagt gttcaatggt
    18241 atgcaaaccg gctgtttcaa tagaattttg gttaggctgc actaggcgaa catcaaaatt
    18301 tgtaatacga tcgcccttgg gtccaaattc ttcagaaata agacgaacat aaggggcctt
    18361 tacaatagta tgatctagct caaaactttc gacgataact tcttttgtca tttcaatctc
    18421 ctttgtttta cactttatca taacacaaat agtgagaaag tggctacttg tggaggagaa
    18481 aaggctaaga aaacagatca aaggctatca taatgacctg tgcgataaaa gtacttacag
    18541 aaacagtaat aaaggtttct ttaaaagcat ttttgtggtt aagtcctgtc aatgctaaga
    18601 aggtgagaaa gacaccactt tggggcacaa tggtaaagat attagaagca atagtggcaa
    18661 cacggtgaat catttcggga ttaaggcctt gatctaaata atactgggca aagttaggca
    18721 tgacaattcc tagagcaccg gaagaagagc cggtaatagc tgacatagag gaagtcaaca
    18781 cggctaaact aataagagga ttaccgggaa tattcaaaat gagatctgag aagaatgtga
    18841 aacctggggc aatcatcaca acggctccga aggcaactgc agatgctgtc gcaaaaatag
    18901 gtgcaataga gctgctagct cctaaattaa agacagcaat cttattagga ataaactgtc
    18961 taaagagcca gcttgcggtg agaatggcag ctaaaagtgc tatgaaaata atgttcttct
    19021 taaaaaaatc gtttccgaaa aggctaccgg ttagggcgat aataatgagc aacaataaag
    19081 gtaagatact ggcaaggaaa tgtgggagat tttttgtttt cacttgtatc tcattgtcaa
    19141 aagcataagt ggcataagtt tcaccccttg ccatactttt tgctagacaa tatttcatat
    19201 aaaagagccc aaaagtcacg cagccaattg agccaacgat actcggaatg gcggcagcag
    19261 ttagtgaggt gtccaagtat tgaataggaa taacattttg aatggcaggt gttccgggta
    19321 aaatcgtcat ggtaaaagtt gcaataccga gccaaagagg aacttgaatt aagttccaag
    19381 ccaagtccat ttttttgaat agacttcgtg caagcggcaa gacagcaaac atgacaacaa
    19441 agaggctgat tcccccgtaa gttaatatag cactgattag aaaaatagca acgagtactt
    19501 tataaggact atcatgacca acttttttta aaatataatc tgcaatagaa gtggtttcac
    19561 cgcttgtttc cattaatttg gctaagatag aacctaataa aaagatagca aaataattga
    19621 gaatataagt ggagagtgcc cccataaatt ggttgggttc ttttccaagt aaggttgtgg
    19681 ttggatccat ttgattaaac aaaatgacca agctggtagc aagaggcgca gcgatgataa
    19741 tgttaacttc tttgacagac aggtaaataa taacaataac acctatcaag acccccagag
    19801 aaccaataat ttccatagtt ttatgacctc cttattagaa aataaagaga cagatcacaa
    19861 gatacagcct ctttatctct ccaaatttat tgagcagtgt agccaccgtc taagatacag
    19921 gcttgacctg taacaccttt tgccttatca ctggcaagga aagacacata gtctgcaatt
    19981 tcttgaacgt caatgaggcg tttttgagga acaagtgggt atagcacttc ttcaagaaca
    20041 ttttcaaggg gaatacctct tgttttggaa aggtcttcaa actggccacg aaccagtggt
    20101 gtgtcaacat atccaggaca aatggcattg accgtaatgc ctgaatcagc agcttctaag
    20161 gcagttactt tggtcagacc gatcaagccg tgcttagctg aattgtaggc ggatttgcca
    20221 gcaaaaccaa tgacaccatt gatagaagcc atattaataa tacgaccgtg tttttgagct
    20281 ttcattgtag gaaaagcacg tttaatggca ataaatggtg ctgtcaacat gattttaatc
    20341 atgaattcaa atttttcagt tggaaaatct tcaatcatgg caacatgttg aaggcctgcg
    20401 ttgttaacca aaatatcaat acgaccatat ttttcaacaa ccgtatcaat agcagcattg
    20461 atggcttctt ctttggtgac atcgcacaca acactaaaag cgtcatagcc ttctttagta
    20521 tagtcagcaa caacctgatc cagtttttct tgattaatgt cagaaaaaac aaccgtttta
    20581 ccttctttta agaaggtttc cccgatttgt tttccaatcc cgcttgcagc accagtgata
    20641 aatgcaactt ctttagtatg tgtcatgata ctatcctcgt ctttcttttg gttagtcttt
    20701 agtttgtttt atcaattagg gtaacttcgg taacgttttg cacgtcttca aaggtgtaat
    20761 ctgggttaat ttcaagggcg catagacctt catcttgata ttcaaagact cccatttcag
    20821 taatgattag gttaacaaca ttttgagcag tgagaggaag ggtgcatttg tctaaaattt
    20881 ttgctttgcc tttattggta tgctccattg caacaatgac ttttttagca cctaccaata
    20941 agtccatggc ccctcccatc ccaggaacca tttttccagg aatcaggtaa ttagcaatgc
    21001 tagcttcttt atcaacttgt aaggcaccta agacggttgc ggccacatgg ccgcctcgaa
    21061 taataccaaa ggaagttgag ctatcgaaga aagcccctcc aggagcaata ctaacaggtt
    21121 gcccaccagc attaacaata ctaggatcgt agtgatcatc tgttaatcca gtgagaccga
    21181 caaaaccatt ttcggactgc agggtaatgg ttacaccttc aggaatatag ttggctactt
    21241 ttgttggcaa acctatgcct aagttgacca gtgtattgtg ttctaattct tgtgcaacgc
    21301 gcttagcaat acgtgtttga atctcttctt ttgaaagaac agtagctgat gttcccatgt
    21361 taggctcctt tcactaagta gttaacaaaa atgccaggag tatggacaaa attaggatcc
    21421 atttgcccca cgtcaacaat ttctcttgct tcaacaatgg tggttttcgc attagcagcc
    21481 atcacatgat taaagttatt ttcagaacct gcgtattgca aattgccatt tttatctgcc
    21541 ttgttagcaa aaataagtgc gatatcagct ttcaaaggtt tttcaagaag gtagtctttg
    21601 ccgtcaacag taatgacttc ttttcctttg gcaacttcgg ttcctattcc agtaggtgtt
    21661 aagaaaccgc ctagcccaaa accgccactg cggatgcgct cagctaatgt tccctgaggt
    21721 acaagatcaa ttacagtctc gccctcagtc atttgtcggc ccgcttcacg gttgagaccg
    21781 atatgtgatg caataatagt tgaaaactgt ttattggcaa ccatcttacc aacgcctttg
    21841 tcaggaaaac cggcatcgtt acagattaaa gtgaggtctt tgacaccttt ttccacaagt
    21901 gtatcaatta atttttctgg tgtgccattt gtcatgaagc caccaaccat aatggtatca
    21961 ccatctttaa cgtgagctac cgcttctttt attgtgattt ctttacacat tttttctacc
    22021 aactttcacg agactaagta ttgtttttca cgataacagc agttccttgt cctccaccaa
    22081 tgcaaagtgt tgctagtcct ctggttactt ggcgtttttt catttcgtgg attaatgtga
    22141 ctagaatgcg agcgccactg gcaccaatag gatgacctaa agcgatggct cctccattaa
    22201 cattcacgat ttctgggttt aactttaaat cttttaaaac cgccaaggac tgtgctgcaa
    22261 atgcttcatt ggactccact aagtccaaat cagaaattgt taatccagct ttcttaaggg
    22321 ctttttgagt ggcaggaatt ggtccagttc ccatcagttc aggtgcaaca cctgcactag
    22381 catagctttc aatagtgact aaaggtgtta accctaattc aagagctttt tcttcagtca
    22441 ttaacatcaa taaggctgca ccatcgttta ttcctgaggc attcccagca gtaacggttc
    22501 cttctttagg gaggaaagca ggacgtaatt gctgtaattt ttcaaggctt gtatcaactt
    22561 ttgggtattc atctgttgta acaattaaag ggtctttgcg tcgttgaggc acagagacag
    22621 ggacaatttc gtcagcaaaa cgatgatttt ctaatgcttt agcagctttt tcttggcttt
    22681 ctaaagcaaa ttgatcttgc tcttctcttg agatgccata tttctgagca acgttttcag
    22741 cagtaatacc catgtggtaa ttgttaaaag catctgttaa gccatcatga acaagagtat
    22801 caatcaattg actattacca agacgttgac caaagcggtg ctgagttgag acataagctg
    22861 cttgtgacat gttttcgaca ccaccagcaa taacgatatc agcgtcacca agcatgatgc
    22921 tttgcgcagc taattggatt gctttaagtc cagaaccgca aaccatatct aatgtgaaag
    22981 ctgttttatc ttcaggaatt cctgagtgga ctgcaacttg tcttgccaca ttttgtccta
    23041 ggcctgcatg aaggacgttt cctaggatga cttcatcaac catttcaggc tttacttgcg
    23101 ttttatcaag gatttttttc agtacagttg ttccaagttc aacagcagag acacttttaa
    23161 aggagcctcc aaaagctccg ataggagttc gtaaagcttc aacgataact atgtttttca
    23221 t
//
LOCUS       DAUVUY010000020        22697 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.020, whole genome shotgun sequence.
ACCESSION   DAUVUY010000020 DAUVUY010000000
VERSION     DAUVUY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22697
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.020"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..1171)
                     /locus_tag="VOX58_000714"
     CDS             complement(<1..1171)
                     /locus_tag="VOX58_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HES5877963.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTSGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDSEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAEELDVPALKTIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVM"
     gene            complement(1300..2433)
                     /gene="ugpC"
                     /locus_tag="VOX58_000715"
     CDS             complement(1300..2433)
                     /gene="ugpC"
                     /locus_tag="VOX58_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HES5877964.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            complement(2731..3579)
                     /locus_tag="VOX58_000716"
     CDS             complement(2731..3579)
                     /locus_tag="VOX58_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HES5877965.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLFETSQFIYLNHQQALPDDLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQSLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
     gene            3919..5241
                     /gene="ska"
                     /locus_tag="VOX58_000717"
     CDS             3919..5241
                     /gene="ska"
                     /locus_tag="VOX58_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HES5877966.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSQPAHGGKTEQGLSPKSKPFATNSSAMPHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKAVHNSAERVNVNYEVSFVSETGNLDFTPSLKEQYHLTTLAVGD
                     SLSSQELAAIAQFILSKKHPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQ
                     AYGINKKSGQEEKMNNTDLISEKYYILKKGEKPYDPFDRSHLKLFTINYVDVNTNKLL
                     KSEQLLTASEHNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHNDTNRIIT
                     VYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK"
     gene            complement(5339..5782)
                     /locus_tag="VOX58_000718"
     CDS             complement(5339..5782)
                     /locus_tag="VOX58_000718"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HES5877967.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     SQLYDSFNEQLAEFVPVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(5797..8016)
                     /locus_tag="VOX58_000719"
     CDS             complement(5797..8016)
                     /locus_tag="VOX58_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HES5877968.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKTDVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLMHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(8268..9545)
                     /locus_tag="VOX58_000720"
     CDS             complement(8268..9545)
                     /locus_tag="VOX58_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HES5877969.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEVSTAAM
                     TKEERQQEIEKIKEDIKKEYPKASEEKFWESSFWGRRYFNEQEYLKSLQSFTEQRLQD
                     IVNLIEKGATKGDKGDAGPKGERGPAGPQGPVGPKGEAGKVGAQGPKGDPGAPGKDGA
                     KGEKGDKGDTGERGEKGDIGATGAQGPQGEKGETGAQGPAGPQGEAGKPGEQGPAGPQ
                     GEAGKPGEKAPEKSPEGEAGQPGEKAPEKSPEVTPTPETPEQPGEKAPEKSPEVTPTP
                     ETPEQPGEKAPEKSPEVTPTPETPEQPGEKAPEKSPEVTPTPEMPEQPGEKAPEKSPE
                     VTPTPEMPEQPGEKAPEKSKEVTPAPEKPADKEANQTPERRNGNMAKTPVANNHRRLP
                     ATGEQANPFFTAAAVAVMTTAGVLAVTKRKENN"
     gene            9927..10409
                     /gene="nrdI"
                     /locus_tag="VOX58_000721"
     CDS             9927..10409
                     /gene="nrdI"
                     /locus_tag="VOX58_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HES5877970.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            complement(10803..11621)
                     /locus_tag="VOX58_000722"
     CDS             complement(10803..11621)
                     /locus_tag="VOX58_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HES5877971.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPNIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMADETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLGWR"
     gene            complement(11704..13890)
                     /locus_tag="VOX58_000723"
     CDS             complement(11704..13890)
                     /locus_tag="VOX58_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HES5877972.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHESAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGALASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDHNTQATPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKIGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGAVIPEVNMSQLT
                     SKPTPAKDFKHVTEEVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTHGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTTEIQDVTLTSLGAQPAKTKVATVEL"
     gene            complement(14247..14996)
                     /locus_tag="VOX58_000724"
     CDS             complement(14247..14996)
                     /locus_tag="VOX58_000724"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HES5877973.1"
                     /translation="MQQYFIKGKAEKEVTITDKDTIKHMFQVMRLADKAEVVLVFDDG
                     VKYLAKVTNSMAHELEIIEALPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKAEFLKSLSG
                     FDHIFVAYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPTEITQFEAAGAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            complement(14996..15949)
                     /locus_tag="VOX58_000725"
     CDS             complement(14996..15949)
                     /locus_tag="VOX58_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HES5877974.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIGQKD
                     RFGELYPDVEQSDMIAIIAYYPSSTNLADVIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQILRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLIKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMVQGEWNALVFKKTDDISGVIGG"
     gene            complement(16019..16489)
                     /locus_tag="VOX58_000726"
     CDS             complement(16019..16489)
                     /locus_tag="VOX58_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HES5877975.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKVDFGLEWNAQAFEEGQQGLEEAASYAYPRY"
     gene            16690..18447
                     /gene="pabB"
                     /locus_tag="VOX58_000727"
     CDS             16690..18447
                     /gene="pabB"
                     /locus_tag="VOX58_000727"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HES5877976.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKSLDQVGPVIENVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDGITIP
                     NQWVSATQKEAYQKAIETIHREMQQGNTYQVNYTLQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDDFAVISASPELFFKQEGNRLTTRPMKGTTKRGVNSWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQTGSVCVDRLCEVERYSTVWQMTSTIVGDVKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTAFLYRHKQIFDLKTTAKVKHKKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKDNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQLFYNQAGQLLETSIGN
                     LFVQLGRTLYTPPVVVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLKLTHLDALLAKSQA"
     gene            18480..19046
                     /locus_tag="VOX58_000728"
     CDS             18480..19046
                     /locus_tag="VOX58_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HES5877977.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LVLSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGNLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            19079..20347
                     /locus_tag="VOX58_000729"
     CDS             19079..20347
                     /locus_tag="VOX58_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HES5877978.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATIDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            20436..20632
                     /gene="ssrS"
                     /locus_tag="VOX58_000730"
     ncRNA           20436..20632
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VOX58_000730"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            20633..20705
                     /locus_tag="VOX58_000731"
     tRNA            20633..20705
                     /locus_tag="VOX58_000731"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20666..20668,aa:Lys,seq:ctt)
     gene            20846..21286
                     /locus_tag="VOX58_000732"
     CDS             20846..21286
                     /locus_tag="VOX58_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HES5877979.1"
                     /translation="MKIIEYKGNYRDDLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KGDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKHGYQFYDDTHLIKKLI"
     gene            21341..21847
                     /locus_tag="VOX58_000733"
     CDS             21341..21847
                     /locus_tag="VOX58_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HES5877980.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVVHLDQLHFSSNWQERS
                     DHDMIADLSTCLLKQDWIIEGNYANCLYEERMSEANHIIYVNFSRFHCVYRAFKRYLN
                     YRGKTRPDMADNCQEKFDVAFVKWILLDGRSRNQLKKYQSVIQKYPHKTIVLTNQKQL
                     NHYMNTIN"
     gene            22096..>22697
                     /gene="smeZ"
                     /locus_tag="VOX58_000734"
     CDS             22096..>22697
                     /gene="smeZ"
                     /locus_tag="VOX58_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal mitogenic exotoxin SmeZ"
                     /protein_id="HES5877981.1"
                     /translation="MKKTKLIFSFTSIFIAIISRPVFGLEVDNNSLLRNIYSTIVYEY
                     SDTVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKDGDKIAMFSVPFDWNYL
                     SEGTVIAYTYGGMTPYQEEPISKNIPVNLWINGKQISVPYNEISTNKTTVTAQEIDLK
                     VRKFLIAQHQLYSSGSSYKSGKLVFHTNDNSDKYSLDLFYV"
BASE COUNT         6394 a         5207 c         4404 g         6692 t
ORIGIN      
        1 ccatcacacg cgcagctgac actccatttc ccatagcttc cttagcatag ttaagggact
       61 caatatcatt aacctcggtt aagtctttaa aaggataatt ggtcatgcca atctcttctc
      121 cttggtaaat gtaaggggtt cctcgcatca aatgcagtaa aatagctaaa gccttggctg
      181 atttttctcg gtaagtgctg tcattgcccc aaatggataa gacccgtggc aagtcatggt
      241 tattccaaaa aagcgaattc cagccttctc ccaactttaa ttctgtctgc cacttgctga
      301 aaatagtttt aagagcagga acatccagtt cttccgcata atcccacttg ggagcgtcag
      361 gtttatgttg aagacccaca tgctcaaatt ggaagaccat ggataactct ttgttttcag
      421 ggcgagaata ctggcgagca atttcaggcg ttgctcccca agtctcaccg acagtcatca
      481 cgtcatgatt gccaaaggtt gcttgattca tttctttgag gtaatcgtgt aagcgcggcc
      541 cgttacccgt gatttcagaa tctggaacct tcccaatcag gtcaatcacg tccatacgaa
      601 aaccaccgat gcctttagca atccagaaat tcatcatatc gtaaatttta tgacgtaaag
      661 cttcattctc ccagttgaga tctggctgac gtttgctaaa aaggtgcaaa taatattggc
      721 ctgatgcatt atcatattcc caagctgagc cactaaaaat agaagtcaaa tcgttaggtt
      781 catctcgcca aatataaaaa tcacgctcag gactattagg gttttcccta gcttcaacaa
      841 accaagcatg ctcatcagac gtatggttga ccaccaagtc catgataatc ttaatgcctc
      901 gctcattagc tgctgctaag agctcatcca tatctgccat gtctccaaaa acatcagcaa
      961 tcgcttcata gtcagagatg tcataaccat tgtcatccat tggactttgg taaacaggag
     1021 ataaccaaat ggctgtaatg ccaagttttt gcaagtagtc cagttgactg gtaatgcctt
     1081 ttaaatcacc aataccattg ccgctggtgt ctttaaagga tctcgggtaa atctgataaa
     1141 tcgtagcttt atgccaccag tgtttcttca ttattcttct cctattaatc attcattcat
     1201 cttttatttt aacgtttttt ctctttttgt gcaatcgttt gcgcaaatga catgataaaa
     1261 aggttggaac ccaacctttt tatgaatatc gcttacacct taacgaatag cttgttcggt
     1321 atctctgtca aagaaatgcc ctttagccac attaaaggtc aagctaactt gctcccctgg
     1381 actatggaaa tcacgcgcat ctacacgaga agcaaactca gtttgtccaa gcttgacata
     1441 gagcatcgtc tcagagccca gtaactcaga aaccaaaact tctgctgtga cagttgcact
     1501 tggataagta tcatgcacga tttgtctact agaaatgtct tctggacgaa taccaaaagt
     1561 gacttttttc cccttgtaac cagccgcttc aagcattttt gcttgacctt caggaatcgc
     1621 aatagcaaga ccatcttcac tcacaatgcg gccatcttta acttccactt caaagaagtt
     1681 catggcaggg cttccgataa aaccggcaac gaatttatta gctggaaggt tatacaattc
     1741 ttgtggtgaa cccacttgct caattttacc aatcgttcca ttaccttgag ggtttttagt
     1801 ggcactcatg ataacgatac gatctgccaa agtcatggct tctgtctggt catgggtaac
     1861 gtaaatagtt gttgagccga tacgacggtg aattttagca atttcagcac gcatggagac
     1921 acgaagcttc gcatccaagt ttgacaaagg ctcgtccatc aagaacactt tagcatcacg
     1981 gacaatagca cgtcccatag ccacacgctg acgttgacca ccagacaggt cagcaggttt
     2041 acgctctaaa aattcggtca aaccaagaat ctgcgcagct tccttgacac gacggtcaat
     2101 atcatctttt ttgtacttac gtaatttcaa accaaaggcc atgttatcat acacggtcat
     2161 gtgtgggtaa agggcgtagt tttgaaagac catggcaata tcacggtctt taggagactt
     2221 gtcattcacc acttcgccac cgatttttaa ctctccttca gaaatgtcct caagacctgc
     2281 aatcatacga agagtggttg atttaccaca tcctgaaggt ccaacaaaga cgataaattc
     2341 tttgtcttta atgtccaagt caaaatcttc aacggcataa tgagtagtat ttgggtattt
     2401 tttatagatg tgatttagat ttaattcaac catgtataac tcctatttag ccagcaaatg
     2461 ttctagctga ctgcaaagct agaaaccgct gatatctttt atatgatgct tttattataa
     2521 caaaaatagc gctttcaaaa cagagacaaa gcgaccaaac ttctttgggc aacttgcaca
     2581 atagcagggc tagtcaagtc gtaagggacc ataaaccaag aaaactatgt aaacacaggc
     2641 cggtcacagg agccaaaaca agctgaacga tctatcattc tccttatcag ctacagtcca
     2701 ggcagcaaaa aagaaagctc tgtggctttc ctaatatttc aacaaaaaga gatgagcaaa
     2761 ggctaaatca tccaattgct tcaagtggag tcctgattgc tgagcaaatt tatcaagttt
     2821 gtattgcaag gagttacggt gaatatacaa gcgctgcgct gtctgcacca gattgccgtg
     2881 ttctgaccat agcgcgtgaa ccacatcggc catatcactt tgaggactta gggattggtg
     2941 aaactgacgc gtcaatgcca aaaaagtgtg gtgcgacagc aaagaccaca gcatcagtcc
     3001 tgaaaatgtt aataattttc tgcctgattg ttgccttaaa taggccgtta acagctgaca
     3061 ttcctcctca aaacactctc tcaatgtacc cgcagctact tgataccagg catttccaac
     3121 aaacatggtt aaggccaaac caaaatcact ttctagcgtt ggcaggagat cttcaagcca
     3181 gcgcaagacc tttattgaga ttgcttgtcg gcataaaaat gccgtctgag ttgcactgat
     3241 aggcaaaatg acctcaagcc ctgcaatcat ctgttggagc aactcaacta agtcatctgg
     3301 taaagcctgc tgatgattga ggtaaatgaa ctgagaagtc tcaaacagct gagggggatt
     3361 gccctgatga tcaagcaaat accgctgcca aggtgaccct agcggctgca agacctcaca
     3421 ctgacccagc cccacctcaa gtaataagcg ctctttttcg gataaacatg atttgggaaa
     3481 atgcacatat tggtcccctt ctttgacact cacccactct ttatctccta acggatgagg
     3541 gcctacttgc atctctggaa aatagtcttt tagctccata gccattcctt tcatgatggt
     3601 ctttaaacca ttataacaca tgactcttta tcacacagtt cggtttgttg tcagcacgat
     3661 tttgtatttt ctgtcttttt aatcattaaa gctaaataag tgttattcat ttttagcaag
     3721 aacattcaat caaatagcta tttatcggaa gattaattta tgtttatact aaaacaggta
     3781 ttatttctct tttttcatta tcatgacgtt atcattaaga tatcatttaa aaacaattat
     3841 taggtttttg tttgtatcta taaaattatt atgttattct aataatgggg attgaaactt
     3901 aacttttagg aggtttctat gaaaaattac ttatcttttg ggatgtttgc actgctgttt
     3961 gcattaacat ttggaacagt caagcctgtc caagctattg ctggacctga atggctgcta
     4021 gaccgtccat ctgtcaacaa cagccaatta gttgttagcg ttgctggtac tgttgagggg
     4081 acgaatcaag aaattagcct taaatttttt gaaattgacc taacatcaca acctgctcat
     4141 ggaggaaaga cagagcaagg cttaagtcca aaatcaaaac catttgctac aaatagtagc
     4201 gcaatgccac ataaacttga aaaagctgac ttattaaagg ctattcaaga acaattgatc
     4261 gctaacgttc acagtaacga cggctacttt gaggtcattg attttgcaag cgatgcaacc
     4321 attactgatc gaaacggcaa ggtctacttt gctgaccgag atgattcggt aaccttgccg
     4381 acccaacctg tccaagaatt tttgctaagc gggcatgtgc gcgttagacc gtatcaacct
     4441 aaagccgttc acaactcagc tgaacgcgtt aacgtcaact atgaagtgag ctttgtctcc
     4501 gaaacaggaa atttagactt tacgccatcg ttaaaagagc aataccattt gaccacactg
     4561 gcagttggtg actctctttc atcacaagag ttagcagcaa ttgcccaatt tatcctatca
     4621 aaaaaacatc cagattatat cattacaaaa cgtgactcct caatcgtcac tcatgacaat
     4681 gacattttcc gtacgatttt accaatggat caagagttta cttaccatat caaagaccgg
     4741 gaacaagctt atgggatcaa taaaaaatct ggtcaggaag aaaaaatgaa caacactgac
     4801 cttatctctg agaaatatta catccttaaa aaaggggaaa agccgtatga tccctttgat
     4861 cgcagtcact tgaaactgtt caccatcaat tacgttgatg tcaacaccaa caaattgcta
     4921 aaaagcgagc agctcttaac agctagcgaa cataacttag acttcagaga tttatacgat
     4981 cctcgtgata aggctaaact actctacaac aatctcgatg cttttggtat tatggactat
     5041 accttaactg gaaaagtaga ggataatcac aatgacacca accgtatcat aaccgtttat
     5101 atgggcaagc gacccgaagg agagaatgct agctatcatt tagcttatga taaagatcgt
     5161 tataccgaag aagaacgaga agtttacagc tacctgcgtt atacagggac acctatacct
     5221 gataacccta acgacaaata accacgatct tctaaaacga tgagattaac tgacaaaaaa
     5281 agcaagcaac acgctatcaa cagttgcttg cttttttcta acctcttagt tgtagagact
     5341 agtgacattt cgtgtctaaa ataatcgtaa ctggtccatc attgatgaga ctaacctgca
     5401 tatctgcccc aaaaacgcca cgctcaactg gcacaaactc tgccaattgt tcattaaagc
     5461 tatcatacaa ctggctagcc aaatctggct tagcagcacc agtaaaggca ggacggtttc
     5521 cctttttggt gtcagcataa agggtaaatt gcgacacaga taagatactg cccttgatgt
     5581 cttggataga ctgattcatc ttgccatcag catctgaaaa aatgcgcatg ttgactattt
     5641 ttcgcacagc gtaagccaaa tcttctgcat tatcatctgg tcccacacct actaacaaca
     5701 acaacccttg gttaatagcg ccagcaattt tcccatcaat agaaacagag gcctctttga
     5761 cccgttgcaa gacaagtttc atggttttat cctttcttag ccattggtcc gcttcacgct
     5821 ataaacatct ggaactgcct tgattttttc gacaacagtg gtcagatgca tcagatttgg
     5881 aatgccaaag ctcacgtgaa tattagcaaa cttcatgtcc ttggtcggct gagcattgac
     5941 tgtcgatatg ctcttggttg agtttgataa aatttggagc acatcattga gcaggccact
     6001 acgattgagc ccatagatat caatttcagc ctgataatct ttactcgaat tgtccaaatc
     6061 ccactcgacc tcaatcaagc gttcttggta gccatcttga ctcttaatgt tatgacagtc
     6121 cgatctgtga atcgcaatgc cacgcccttt ggtaatatag ccgtcaatag gatcaccagg
     6181 tacaggatta caacacttgg caatccgcat caagaggcct gatgctcctt ggataatgac
     6241 cccattttca ctgcgaacct tgagcacatc tttgttttcg tgtttgacct caccgccctt
     6301 aaccaattct tcagcttctg ctttagcctt ggccctttct tcttcacggc gctctttttc
     6361 ggttaacttg ttaaagacac tgataggact aatgtcacca aacccaacgg ctgcatagag
     6421 tgattcttcg ctcttcacac tgacttttgg aaggatcgct tcaatgcgtt ttttgtcaag
     6481 gtatttattg gcaacatagc cctgctcttg aaaataagac accaacaaat cacggccttt
     6541 attcactgac aattccttgt cttgattttt aaagaactga cgaattttgt tgcgagcctt
     6601 attggttttg accagtttta cccagtctcg actagggcca aaggaattgg cattggtgat
     6661 gatttcaacc acatctcctg tttttaactt ggcagtgaga ggaaccatac gtccattgac
     6721 tttggcacct gttgcttttt caccgatttg cgtatggatc gcataagcaa aatcaatagg
     6781 acctgattct tttggcaact cctgaacggc ccctgtcggt gtaaagacat aaatccgttc
     6841 agaaaaaatg tcttctttga ccgaatccac aaagtccact gcatcgccat ttgaggcatc
     6901 ttgcaattct accagctctt tgatccagtt catgccaacg gcttgctcag cctgattgac
     6961 cttaccacgc acgccttttt tataagccca gtgagcagca accccgtact cagccacttg
     7021 atgcatgtcc ttagttctga tttgaatctc aataggtcct tttggtccat acacagtggt
     7081 atgaatcgac tggtagccat tagctttagg agctgcaata taatccttga agcggcctgg
     7141 catgggacgc caaagctcat gaatatagcc aaccatagca taaacatcgc tctgcgtttc
     7201 catgacacaa cggatagcaa tcaaatcaaa aatctgatcg aaacgtttct ttttgtcccg
     7261 cattttccga taaatcgaat aaatgtgttt tggtcggcca tacacatctc caaacaaccc
     7321 ttgttgtgtc gtataggttt tgaccttact gacaatagcc tctaccaaag cttcacgctc
     7381 gcgacgtttt tctttcatca tatgggaaat tttgtaaaat tcggtttcat tgaggtaacg
     7441 aaaagccaaa tcttctagtt cccatttgat gcgactaatc cccaaacgat gcgccaaggg
     7501 ggcatagatt tccatggttt cgcgcgaaat gcgctcttgt ttgtccttgc gcaaatgttt
     7561 gagggtgcgc atattatgca ggcggtcagc caatttcacc aaaatcacgc gaatatcttt
     7621 agacatagcc atcagcattt tgcgatggtt ttcggcgagt tgctcctcat gagatttgta
     7681 ctcaacttta cctaacttgg tgacaccatc aacgatatca cgagcatctt ggccaaagtc
     7741 tgcttcgatc tcatctaagg taatatccgt atcttctacg acatcatgta aaaagccaca
     7801 ggcaactgtc acagcatcca gatgcaaatc agccagaatc cccgccacct gaatcggatg
     7861 gacgatatag ggttcgcctg actttctcac ttggtagaaa tgggccgctg ttgcatatgc
     7921 taaagccttt gccacaaaag ccacatcagt cttggtcata taggtggccg ctaaggcaat
     7981 gacttcttct cctgttacgt tcattatttt tgccatttcg ttcctcatct agcatttctt
     8041 ttccctattt taacacttta cacacaaaag caaaaggcag aatctgctaa gaaagcgctt
     8101 agtgtgacct tttcttagaa aacggttagt gagctgcttt tgatatcttt tgggatctct
     8161 caggcgtgag catgatacca acggccagct cccgaccaaa aatagagaga accaaggggc
     8221 tctctctatt atgtttgctt atcagtaagg tctctcaatt cagaggatta gttgttttct
     8281 ttgcgttttg taacggctag gacaccagct gttgtcatca ctgctactgc tgctgctgta
     8341 aagaatgggt tggcttgctc accagttgct ggtagacgtc tgtggttgtt ggctacaggt
     8401 gtcttagcca tattgccatt gcggcgttct ggcgtttggt tagcttcttt gtcagcaggt
     8461 ttttctggag ctggagttac ctctttgctc ttttctggag ctttttcacc tggttgctct
     8521 ggcatttctg gcgttggtgt cacttctggg ctcttttctg gagctttttc gcctggttgc
     8581 tctggcattt ctggtgttgg tgtcacttct gggctctttt ctggagcttt ttcgcctggt
     8641 tgctctggtg tttctggcgt tggtgtcact tctgggctct tttctggagc tttttcgcct
     8701 ggttgctctg gtgtttctgg cgttggtgtc acttctgggc tcttttctgg agctttttca
     8761 cctggttgct ctggtgtttc tggcgttggt gtcacttctg ggctcttttc tggagctttt
     8821 tcgcctggtt ggcctgcttc gccttctggg ctcttttctg gagctttttc acctggtttg
     8881 cctgcttcac cttgtgggcc tgctggacct tgctcacctg gtttgcctgc ttcaccttgt
     8941 gggcctgctg ggccttgtgc gccagtttca cctttttcac cttgtgggcc ttgtgcgcca
     9001 gttgctccga tgtcaccttt ttcaccgcgc tcaccggtat cacctttgtc gcctttttca
     9061 cctttggcgc cgtctttacc tggggcacca ggatcacctt taggaccttg ggcaccaacc
     9121 ttacctgctt cacctttagg tcctaccgga ccttgtgggc ctgctgggcc gcgctcgcct
     9181 ttaggaccag catcgccttt gtcacctttt gttgcacctt tttcaatcag atttacaata
     9241 tcttggagtc tttgctcagt aaaactttgt aaagatttta gatactcttg ttcattaaag
     9301 taccttcttc cccaaaagct actctcccaa aatttttcct cagacgcttt tggatattct
     9361 ttcttaatgt cttctttaat tttctcaatt tcttgttgtc tctcctcctt cgtcatagcc
     9421 gcagtagaaa cctcagcctt aacgctgctt gccccgcctc caaaggctag aaggacggca
     9481 gcggctgagg ttagcccgta gcgccagact agtttgttga gattgtggtg ttttgatgtc
     9541 aacatatgtt gttctctctt tctttattag atggtgtgtt ttagataaaa ataacatggt
     9601 agctagacct gattatttat aatagtatta taatagagtg tgaaaacgat tgtaaagttc
     9661 tcttttgttg ttcatctttt agattttgac tgatcactct tttgtggaga tcagagggct
     9721 actttggcac ttgcctgcct cgtttttaag tcgagggcat ccggatcctt tctttaaggt
     9781 cactcttttt tgaaatgtta ctttttttga ccgcttgtac cttcttgtct ggttggatat
     9841 cgctaggttt cttctttact tgtttgttgg tcttgattgt ggtaagctta tgaagaaacc
     9901 ttgtaggata aaaaaaggag acacttatgc ctcagattac tcttgttttt attagcctca
     9961 gtggcaacac ccttagcttt gtcaagcggc tatcccttta tttagcagac aattacgatt
    10021 accatgtcaa acagattaac attaaagatt tgaagcacga gacttttccc gtcaaggaag
    10081 agtttgtggc gattctaccc acttatctag aaggcggaaa tggcgttgac tctggtgagg
    10141 tggagatcct caccactcct ttaggagagt ttattgccgc ccatggcaac gcccagcgtt
    10201 gtcttgggat tattggttca ggcaacaaaa actttaacca tcagtattgc ctgacagcca
    10261 aacagtatgc caaacgcttt ggctttccac tgctaggtga ttttgagctg cgagggactc
    10321 ctgatgacat ttcgcggtta gcccaggtca tcatggaggc cagctccaga cactcgtcta
    10381 acgacacaca gacactgcct aacagctaga ccgtctatag atgatcatag ctgttttaaa
    10441 aaagagcact tttaggacac caactaggga ggtctcttac cctacgcctt gcgatttctc
    10501 agcctttgaa aagagcgatc acagtgcttc ttggggcttg ggatcactcc aagcgctcct
    10561 agaaataccc ctatgcagac tttagcaagg caactgaacg cttggcggta ctttctgtga
    10621 agctcagatg gttggtggca aaaaagccct ggttttttag ggttactgac atgactaaag
    10681 aggtctaacc agctctacct tgagctcgtt agacctcttt gtgttctcct aatcccccta
    10741 gacgatcgca gtttcttatg tgtcttggct tctcctatgt ggtgtgcctg ttagcacatg
    10801 ctctaacgcc agcctaatgt cacttctaaa ccgtagtggt cactgacgac tggcgcatct
    10861 ccgccttcaa aggtaatctt agaagatcgg atgatgaagt ccttgctggt aaagacatgg
    10921 tccaccttga gggcttcttt gttaccttgc cagccatcaa tatcagccac gatgctatga
    10981 tcaccaaaca catggtcagc aaccttatgg ctatcttgta ggtctagggg gctacccatc
    11041 atcacttgat aaccgtcttg atccgttgga ttgttaaaat ctcccataag tagcaaggga
    11101 cagtttagtg tgagcagttc tttttctaat ttctcccact cccctaaaaa ccctttgtca
    11161 aaccaagata ggtgcacatt gacaacagcg acttctttgc catctaacgt ggttttggct
    11221 atgagtgagc gtctggtgtg gtagtctgtt tcatcagcca tcgctgacac gagaatatct
    11281 gaaacatgaa taggttgttt tgataagatt gccacgcctt cctggtaaat gtcatagcca
    11341 atatggttat aagcccaaga ccaataatag tgctgccctc gtttttgtaa ataatggatc
    11401 aagagcaggg caaaatggtc tttatgaatg tttggtgtcc ctgaaagagc ttgatatctt
    11461 ggtaaatctg tggccagctc gctctcgatt aactgattga tttcttgcaa gcaaatgata
    11521 tcgtattttt cagctaaaat atgctcagcc aaagcaacta attttttaag agtgttggct
    11581 tgcatccacg aatgggtatt cagtgttaat accttggtca ttaatcctcc tttataagac
    11641 aagaagagcc atagggccta aaccagctat ggctattacc tatcactatc tcgtgatgtt
    11701 ttgttaaagt tctacggtag ctactttagt cttagcaggc tgagctccta aagatgtcaa
    11761 ggtcacatct tggatctctg ttgtgttggt aaaagcaaca acaatggttg tttcacgctc
    11821 tgctgattta atagcagcaa gatctgctac gactaatagg tcacctgcat gaacccgttg
    11881 tccttctgag actttgactg aaaaaggcac accatttaag gcgacggtat caagtcccac
    11941 gtgcaccagc acttcaagac cattatctgt cagtaaacca aaggcatgct tggtcggaaa
    12001 gacactggtc acaaggccag ctactggtgc atagatatta ccatgggttg gttcaaccgc
    12061 aaacccatct cccatcatct tagccgcaaa cacctggtct ttcacacctg tgattgggag
    12121 aaccatcccg tctgccactg atagcacttc ttctgtcacg tgtttaaaat cttttgcggg
    12181 agtcggtttg ctagtgagct gtgacatgtt gacttctgga ataacagccc ctgagtccag
    12241 caaatcttga atatctgatt tcaaaatatc tgctttaggc ccatagaccg cttgaacgcc
    12301 gttgcctttt tggatcaagc ccatagcgcc agcttttttc caatcgtcct cagcgccaat
    12361 cttagcgggg tctttgaccg tcactcgtaa gcgcgtcata caagcatcaa catcatcaat
    12421 attgcctgcc ccaccaagga gattaatgat ttgtacgact tgagagttgc tatctgctac
    12481 ttgggtaggt gttgcttgag tgttatggtc acccagtata tcggcatcgt agttgcctaa
    12541 acggccagct gtcgcgagat gcattttctt gatcatcata tccgcaataa agtacatgat
    12601 aactgcaaag aggatagaaa cccaaacaaa gttaatcaca tccatgccta gacctgcttt
    12661 aagggccata ggggtacggg ttagtagctc aatgttccca aatgagtgaa cacggagatt
    12721 cacaaggtct gccatggcaa atgaagctcc ttgaacaagg gcatagacga tgtaaagtgg
    12781 cattgctgca aacataaata ggtactcaag tggctcagtt acccctgtca aaaagaccgc
    12841 tgccgctgct gagatgaaca tcattttgta tgtatgtttt ttatcggcat ccacattacg
    12901 gtacatggct aaggcaaccc ccatcaatgt tccggtagct ccaatcattt gtcctacctt
    12961 aaagcgagct ggagtcacac tatccattaa gtgactatag gctgaagcgt ctgatccttt
    13021 aaggtgaaca aggtcagtta cccaagcaag ccacaaaggg tcttgaccaa atacttttgt
    13081 ccccgctgct gcacctgtca tgacctcata agttccacca agagctgtat agttcattgg
    13141 aatcgtcaac atgtggtgaa gaccaaatgg caacaagaga cgttccaagg taccatatag
    13201 gaatggggct aggattggag ctgaatcttg tgaagaggca atccacatcc caaaactgtt
    13261 aatcccagac tggatcaccg gccaaacaac aaccaaaata agggctacaa aaatagaacg
    13321 taaaataacg acaaacggta caaaacgctt cccattaaag aaggtcaaaa cttcaggaag
    13381 tttacggtaa ttatagtatt tgttataggc cgttgctcct acgaaccctg caataatccc
    13441 aacaaaaacc cctgtgttta aagctggaga ctccaagaca ctggtgaaat aatctttaac
    13501 gatcatttga gtgccaagga ggcttgtgat ttttgcttct ggatcagcta gcatagcact
    13561 agacacgcca taaaaagcac ctgttatccg attgatcaag acaaaagcaa gccctgaggc
    13621 aagggcccct ccagctcttt ctttagccca actaccacca atggccaagg caaatagcaa
    13681 atgaaggtta acaataacag cccaaccaat ttgagcgata atgttgccta gagatgccaa
    13741 aaatgctgat tcgtggttaa tcattggaat agagtttccg atactgatca ttaaaccagc
    13801 cgctggcatg acagcaataa cgaccataag acatttccca aatttttgcc aaaattcgaa
    13861 acgaaacagt tgcttgaatg atgttttcat tctatctctc ctttattatc cccttttagc
    13921 catagctgcc cttaaggcgc ttttgtgaca caatgatata gactgcaact cagtgatgca
    13981 atcgtttgca ttgatctgat acctttatta tactagcctc gcaacaattt gtaaagcgtt
    14041 ttcttagaca gatgttagtc gtttttcttc attaaatggt agtgtaactt accaaaaaga
    14101 taacaagagc ccatttcctt ttttggtgat gagaccatca acgctcttcg aaaaaacaag
    14161 aagcaataaa aaaaggccgt tgacacaatc gtgaactgcc tgttcgtttc tttagcctct
    14221 tctttctaaa gtaagcagca gcttacttat tttgatgcct gcaattccaa agcataacta
    14281 agagcactta aggcatatag cggcgctgtc tctgctcgca taatgcgagg gcctaagccc
    14341 accttgatgg cacctgctgc ctcaaactga gtgatttcag ttggggaaat tcccccttct
    14401 ggtccaaaga taaagaggat ttttgcccct ggcttaactt cttttacttc acgcgccaag
    14461 gtcgctagct gacctgcctt agctgtttct tcgtatgcaa cgaaaatatg atcaaagcca
    14521 gaaaggcttt ttagaaactc tgctttgtgc tcaaaaaggt gaacttcagg caccctattt
    14581 cgtttgctct gctcagccgc tcctaataca atcttagcca atttgtcttc ttttttagct
    14641 aattttttgc catcccattt gaccactgac cagtctgctg ggtagcccca tagcgcactg
    14701 gctcccaatt cagtcacttt ctgtgcaatc gtgtctaact tatcgccttt aggaaatcca
    14761 gaagcaatag tcaccttaac aggcaattcc acctgatctg gcaaagcctc tataatctct
    14821 aactcatgag ccatactatt ggtcaccttg gctaaatact tcaccccatc atcaaagacc
    14881 aagacaactt cagccttgtc agccaagcgc atcacttgaa acatgtgctt gatggtatct
    14941 ttatcggtaa ttgttacctc tttttctgct ttgcctttaa taaaatactg ttgcattagc
    15001 cgccaatcac tcctgagata tcatctgttt ttttaaagac aagggcgttc cactcacctt
    15061 gaaccatatg cgtctcaagg aagaatcctg cgctaaaggc agcttccaga accatatcca
    15121 atttctcaga aatgatgcct gataagataa ggtaaccttc ctttttgatc aaacggtaag
    15181 catcatcagt caataagact aagatatccg ccaagatatt ggctacaatc acatccgctt
    15241 cttggctaac cccttttaaa aggtcaccag cagccacatg aatgttgtct gttccttgat
    15301 ttaaatcaat gttttcttga gccactcgaa cagctacatc atctaagtca taagcgtaga
    15361 tggttttagc acctagtaag gaactggcaa tcgagaggac acctgatccg gtccctacat
    15421 cgataactgt ctcaccacca cgaaggattt gttctagagc aaaaaggctc atcttagttg
    15481 tgggatgtgt ccctgtccca aatgccattc caggatctaa tttaataacc ttttccccag
    15541 cagaagcgtc ataatccgtc caactaggga caatagttaa atcatgcgtg atgcgagctg
    15601 gttcatagta ttttttccaa ttgtctgccc agtcttcctc agctaattct tgactatcca
    15661 cagtaacctg accaacctgt aacccaaaac tagctaactc cgctaactgt tcattaatgg
    15721 tcgcaataac atccgccaaa ttagtgctac ttgggtaata ggctatgata gctatcatgt
    15781 cagactgctc cacatctggg tataactccc caaagcggtc cttttgccca atatagtctg
    15841 cgctatccgc gatagcgacc ccttggctgc ctgtctcaat caacacatgt gaaacagcct
    15901 cttgcgcatc acgatgcaca tgaaccgtca cttcttgcca cgtttccata agtatgtcct
    15961 ttctattttg accttttggt tattgtccta atggtgttac accatctcgt gtgtcacctt
    16021 agtaccttgg gtaagcgtaa cttgctgctt cttctagtcc ttgttgacct tcttcaaaag
    16081 cttgcgcatt ccattccaag ccgaaatcaa ccttgctgtc atctgctaag aaatccatca
    16141 aatcgctgtg cccttcaata gcaacatcgt tgagaaattg cgcaaagtaa gctaagaatt
    16201 cacgagaaaa gcccttttta gcgtcaaaag gaatagtaac caaataatct gctgcatcaa
    16261 attgagactt ggcagggttg taaaagagca catagtcctc aaagacaata tcatcttggg
    16321 tcacttcgcc actgtcgtca atggttttta tggcctcttt attttgagct tctaacacaa
    16381 aagtcacttc aaccgcatga tttttcttgt cccaatccat agcataatca tactgaaaat
    16441 gcttatccat ttcttcttct aatactgata aaaagccgta ttttgccatc tttttttata
    16501 cctaatcttt ctaactgttg ctgtatagag ctttgtaaga cctcattagt ttacttactt
    16561 tgacatgctg agcgtactgt tataactttt attaactgaa ttataacata tttgactaat
    16621 attgaccata agagaaggtc ttaatggctg gatcacctct agcaaccgct tttatgatat
    16681 aatttgggta tgcatagaaa aacgattatt gatttcaaag agctcgggca acgctatctt
    16741 tttgatgagc cattagtaga attggtagcc aagtccttag atcaggtcgg tccagtcatt
    16801 gaaaacgtac aacactacca acagctgggc tattacgtgg tgggctatct cagttatgaa
    16861 gcagctgctt tttttgacaa tgccttacaa acccataatg acagactagg caacgagtac
    16921 cttgcctatt ttacagtgca caaaacctgt caaaaaaaag acctacctct tgattatgac
    16981 ggcattacta tccctaacca atgggtcagc gcaactcaaa aagaagccta tcaaaaagcc
    17041 attgaaacca ttcaccgtga gatgcaacag ggcaacactt atcaggttaa ctacaccctt
    17101 caattaactc aggaactcaa tgcagctgac agcttagcca tttacaataa actcgtcgtt
    17161 gaacaggctg ctggctacaa tgcctatatc gcccatgatg actttgctgt tatttcagct
    17221 agtcctgagt tgttctttaa acaagagggc aacaggttaa ctactaggcc tatgaaaggc
    17281 accaccaagc gtggggttaa tagctggctg gatcaacaag aacatgactg gctccaagct
    17341 gacgggaaaa accgttctga aaacatgatg attgtggatt tgctccgcaa tgacatggga
    17401 aaaatttgcc aaacaggcag tgtttgtgtc gataggctct gtgaggtcga gcgatactct
    17461 accgtctggc aaatgacctc aaccattgtt ggtgacgtca aagcggactg tgacctgata
    17521 gacattctca cagctctctt tccttgtggc tctatcacgg gggctcctaa agtctccacc
    17581 atggcaatta tcacatctct ggagccaaaa ccaaggggga tttattgtgg aagtattggc
    17641 atttgcctac ctgatggtcg ccgtttcttc aatgttccca ttagaaccat ccagctcagt
    17701 cataatcaag ctacttatgg tgtcggaggc ggtatcactt ggcaaagcaa gtgggaagac
    17761 gaatacgagg aagtccatca aaaaacagct tttctctatc ggcacaaaca aatctttgac
    17821 ctcaaaacaa ccgccaaggt gaaacacaaa aaaatagcct tccttgagca acacctcaat
    17881 cgccttaaag aagcagccac ttattttgct tacccttacg atgagaaagc tttgcagaaa
    17941 caactgtcaa cctatttgga aaataaggat aatgctgcct accgcttgat gattcgttta
    18001 tccaaggatg gaaagattag cctctctgat caacccttgg aacctttgtc agccgatttt
    18061 ctgacagctc aactctcttt acagaagaag gacgtgacag cttcgccttt tacctacttt
    18121 aagaccagct accggcctca tattgagcaa aaatcttatg aacagctatt ctataaccaa
    18181 gcggggcaat tattggaaac gtccattggc aatctctttg ttcagctggg tcggaccctc
    18241 tacacaccac cagttgtggt aggtatttta ccaggacttt ttcgccaaga gctgctagct
    18301 actggtcaag ctcaggaaaa agaggtgaca ctagctgatt taaaagaggc tagcgctatt
    18361 tttggaggaa atgctgtccg tggcctctat cctctcaacc ttaagcttac tcaccttgat
    18421 gccctcttag ctaagagtca agcctaaact gccactcatc ttttgttatt aaggaaccta
    18481 tgatactctt aattgataat tacgattcat ttacctacaa cctcgcccaa tatttaagtg
    18541 aatttgacga gacgattgtc ttgtataacc aagacccaaa cttatatgac atggccaaaa
    18601 aagctaacgc tctagtcctc tcacctggtc ctggttggcc caaggaagcc aaccaaatgc
    18661 caaaactcat tcaagacttt taccaaacaa aacctatctt aggagtgtgt ctgggacacc
    18721 aagctatcgc tgaaacccta gggggaaact tacgcttggc taaacgtgtc atgcatggga
    18781 gacaaagcac cattgaaacg caaggccctg ctagtctttt tcgctccctg ccacaagaga
    18841 tcaccgtcat gcgttaccat tccatcgttg tggatcagtt accaaaaggt tttagcgtaa
    18901 ctgctagaga ctgtgacgat caagaaatca tggcctttga acaccacacc ctgccacttt
    18961 ttgggctaca atttcaccca gaaagcatcg gaactcctga tggcatgacc atgatcgcca
    19021 acttcatcgc agccattccc cgttaatctc atcccccaaa agaaagaagg acagttacat
    19081 gcctgatcat ttagcgctca gaatgcgccc caaaaccatt tcagaggtca tcggacaaaa
    19141 acaccttgtt ggcgaaggca aaatcatccg tcgcatggtg gaagcaaata gactatcgtc
    19201 catgatcctc tatggccctc caggaatcgg taaaacttcc attgcttctg ctattgctgg
    19261 tactactaga tatgcctttc ggacctttaa tgccaccatt gacagcaaaa aacgtctcca
    19321 agaaattgcg gaagaagcta aattttctgg tggcttggta ttattactag acgagattca
    19381 tcgccttgac aagacaaaac aagattttct tttaccttta cttgagaatg gcaccattat
    19441 catgattggc gcaaccactg agaacccctt cttttcagtg acacccgcta tcagaagcag
    19501 ggtccagatt tttgaattgg aaccactttc taacgaggat attaaaacag ccattcagct
    19561 agcaatttcc gacaaagaac ggggatttcc ttttttagtg acaattgatg atgaggcgct
    19621 agatttcatt gttaccgcca caaatggaga cctacgatct gcttataatt ccttggatct
    19681 agcagtcatg tctacttctc ctaatgaaga tagtagccgt catattagtc tagagacaat
    19741 ggaaaatagt ctgcagcgta gttatattac catggataaa aacggggacg ggcattatga
    19801 tgttttatct gccttacaaa aatccattag aggttctgat gtcaatgcta gcctgcacta
    19861 cgccgctcgt ttagtagaag ctggagactt gcctagttta gcaagacgat tgaccattat
    19921 tgcttacgaa gatattggct tagccaaccc tgacgcccaa gttcataccg tcaccgcact
    19981 agatgccgct caacgcattg gttttccaga agctcgtatt cttatcgcta acgtggtcat
    20041 tgatttggct ctatcaccta aatccaattc agcttattta gctatggatg cagctttagc
    20101 tgatttgaga acatcaggta acctccccat tccaaggcat cttagagacg ggcattatgc
    20161 tggcagtaag gacctaggca atgccaaaga ttacctctac ccacacgcct accctgaaaa
    20221 atgggtcaaa caacaatacc taccagataa attagtagga catcattatt ttgaagccaa
    20281 tgaaaccggt aaatacgaac gcgcacttgg ttcaaataaa gagcgtattg ataagctctc
    20341 agactgactg ctagttttaa aaatcgcaac cgatttcaaa aatcgataat tttgcttgaa
    20401 aaaattttaa aaagtggtat tataaaagta cagaaacatt gctgtggcat acgaatccac
    20461 atctaagtgt tgaccgacta tttctttgta ttttcgggaa acaaagaccc tttctagtct
    20521 gcaagccgtt tcacgcggac gcaacttcca agaaagagcg cgttacccac ctgcttatac
    20581 tgcgcgggct caatacaaaa acgtggatca ccggtgccaa tacagcattc ttgcctcctt
    20641 agctcagttg gtagagcagt agactcttaa tctatgggtc acaggttcga gccctgtagg
    20701 gggtatacaa ctatacatga aaaaaccttt gtttaaaggc tttttttgct tgtcagaaga
    20761 ggatttgttc catcatttat ccccgcagta atttttttat ggtatgatac caattaaagc
    20821 gcttaagatt aaggtggaga ctgttatgaa aataattgag tacaaaggaa attacagaga
    20881 tgacctcatt tttatggtct tggaagcaaa aaatgcctta aggagaattc ccagcattaa
    20941 cccggatctt ttgacaattg aaacaagtta tttggaaaaa ggggattgtt tttggctagc
    21001 ggtcaatgaa gaaagtcgtg ttattggctc aatcgcttat tcttctattc ccaaaacaaa
    21061 tgaggtcttc ttgcaccgtc tcttcatcaa accaagctta aaacatcaag gcattggcag
    21121 tcaacttctg acgtttgcag aagattatct aaggaaaatc ggcaagagtc atgttcgtat
    21181 ccacttaggt caacctaaag aacaatggtt tgagtcttat cggttttatc ctaaacatgg
    21241 ttatcaattt tatgatgaca cgcatctcat caagaaacta atctaaacac atttcatacc
    21301 ctttatagtc atttcatacg tcatttttag gaggtatcca ttgaaaattg ccatcattgg
    21361 acatagtggc tctggaaagt caaccttagc acgattttta ggtcaacatt accattgtga
    21421 ggtggtccat ttagaccaac tacacttctc atcgaactgg caagaacggt cagatcatga
    21481 catgatagct gatttatcaa catgcctgct caaacaagat tggattattg aggggaatta
    21541 tgccaattgt ctctatgaag agcgcatgag tgaggctaat cacattatct atgttaattt
    21601 cagtcggttt cattgtgtct atcgagcatt taagcgttat ttaaactata ggggaaaaac
    21661 gcgtcctgat atggctgaca actgtcagga aaaatttgac gttgcgtttg tgaaatggat
    21721 tctactagat ggacgttcaa gaaaccaatt aaaaaaatac caatccgtta tccaaaaata
    21781 ccctcataaa accattgtct taacaaatca aaagcagctc aatcactata tgaatacaat
    21841 caactaatta gagtgaaaaa ctaaaatagc cacgggtaac ccgggctatt cagactgaaa
    21901 gcctttgttt aaaggctttt ttgcttgttt cataactaat cggcttaatt aaaagcgtca
    21961 atccactttt agtatcatgt ctcaattttc atttgatagt cagcaagtat atctacaaaa
    22021 ttgttaatga ttatttcttt tgaaagtgtt atattaaaac ataataaaat aataactcct
    22081 gaaaagaggc tatttatgaa aaaaacaaaa cttatttttt cttttacttc aatattcatt
    22141 gcaataattt ctcgtcctgt gtttggatta gaagtagata ataattccct tctaaggaat
    22201 atctatagta cgattgtata tgaatattca gatacagtaa ttgattttaa aaccagtcat
    22261 aacttagtga ctaagaaact tgatgttaga gatgctagag atttttttat taactccgaa
    22321 atggatgaat atgcagccaa tgattttaaa gatggagata aaatagctat gttctccgtc
    22381 ccatttgatt ggaactactt gtcagaagga acagtcatag catataccta tggtggaatg
    22441 acgccttatc aagaagaacc aatatctaaa aatatccctg ttaatttatg gattaatgga
    22501 aagcagatct ctgttcctta caacgaaata tcaactaaca aaacaacagt tacagctcaa
    22561 gaaattgatc taaaggttag aaaattttta atagcacaac atcaattata ttcttctggt
    22621 tctagctaca aaagtggtaa attagttttt catacaaatg ataattcaga taaatattct
    22681 ctcgatcttt tctatgt
//
LOCUS       DAUVUY010000021        22198 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.021, whole genome shotgun sequence.
ACCESSION   DAUVUY010000021 DAUVUY010000000
VERSION     DAUVUY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22198
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.021"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..798
                     /locus_tag="VOX58_000735"
     CDS             <1..798
                     /locus_tag="VOX58_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HES5877982.1"
                     /translation="YNYRDHRKIVVIDGEVSFTGGINLADEYINEVERFGHWKDAGLM
                     LEGEATDSFLILFLQMWSITEKELIIDPYLSDHSLKLPSDGYVIPYGDSPLDTDKIGK
                     NVYIDILNHAKEYVYIMTPYLILDSEMEHALRFASERGVDIRIIMPGVPDKGVPYALA
                     KTYYKALMTSGVKIYEYQPGFVHSKVFISDNTKAVVGTINLDYRSLYHHFECATYLYR
                     VSVIADIVNDFNEAQKQSLLMTSDHLTQRPWYQKLIGLLVRTIAPLL"
     gene            1138..1476
                     /locus_tag="VOX58_000736"
     CDS             1138..1476
                     /locus_tag="VOX58_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877983.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
     gene            complement(1884..3152)
                     /locus_tag="VOX58_000737"
     CDS             complement(1884..3152)
                     /locus_tag="VOX58_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HES5877984.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYNTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPYFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSYRNFTLDKSDRIIIT
                     GKKRYLSIAINQQIQSKLNSLIKNTCGKSN"
     gene            3242..4108
                     /locus_tag="VOX58_000738"
     CDS             3242..4108
                     /locus_tag="VOX58_000738"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HES5877985.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            4086..4643
                     /locus_tag="VOX58_000739"
     CDS             4086..4643
                     /locus_tag="VOX58_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HES5877986.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRIPIGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            4777..6321
                     /locus_tag="VOX58_000740"
     CDS             4777..6321
                     /locus_tag="VOX58_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HES5877987.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
     gene            6688..7911
                     /locus_tag="VOX58_000741"
     CDS             6688..7911
                     /locus_tag="VOX58_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HES5877988.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLNLINQDITRTQAHQKKRLKKL
                     EDQKASFERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
     gene            complement(7953..9515)
                     /gene="guaA"
                     /locus_tag="VOX58_000742"
     CDS             complement(7953..9515)
                     /gene="guaA"
                     /locus_tag="VOX58_000742"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HES5877989.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            9682..11115
                     /locus_tag="VOX58_000743"
     CDS             9682..11115
                     /locus_tag="VOX58_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5877990.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            11198..11896
                     /locus_tag="VOX58_000744"
     CDS             11198..11896
                     /locus_tag="VOX58_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HES5877991.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            11985..12326
                     /locus_tag="VOX58_000745"
     CDS             11985..12326
                     /locus_tag="VOX58_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HES5877992.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            12341..13903
                     /gene="ffh"
                     /locus_tag="VOX58_000746"
     CDS             12341..13903
                     /gene="ffh"
                     /locus_tag="VOX58_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HES5877993.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQVFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(14038..14736)
                     /locus_tag="VOX58_000747"
     CDS             complement(14038..14736)
                     /locus_tag="VOX58_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5877994.1"
                     /translation="MFSGHQLKTARLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
     gene            15206..16276
                     /gene="xerS"
                     /locus_tag="VOX58_000748"
     CDS             15206..16276
                     /gene="xerS"
                     /locus_tag="VOX58_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HES5877995.1"
                     /translation="MRRELLLEKIETYKAVMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
     gene            16975..17457
                     /locus_tag="VOX58_000749"
     CDS             16975..17457
                     /locus_tag="VOX58_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HES5877996.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
     gene            17546..18598
                     /gene="citC"
                     /locus_tag="VOX58_000750"
     CDS             17546..18598
                     /gene="citC"
                     /locus_tag="VOX58_000750"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HES5877997.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGGGFLNRIVSHLIDEEYALGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAIVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDQSLVPFSVRKMLIQEGLKHLDNIIFHETGPYLISQ
                     ATFPAYFQTEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDSDWDLLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(18708..20102)
                     /locus_tag="VOX58_000751"
     CDS             complement(18708..20102)
                     /locus_tag="VOX58_000751"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HES5877998.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFV
                     QKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLADKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKII
                     GDEAVIACRPADLIEPQMIYLRDEIAQYARSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(20117..20695)
                     /gene="citX"
                     /locus_tag="VOX58_000752"
     CDS             complement(20117..20695)
                     /gene="citX"
                     /locus_tag="VOX58_000752"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HES5877999.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEVFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            complement(20688..>22198)
                     /gene="citF"
                     /locus_tag="VOX58_000753"
     CDS             complement(20688..>22198)
                     /gene="citF"
                     /locus_tag="VOX58_000753"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HES5878000.1"
                     /translation="DIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSV
                     REAIEKTGLTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSIANVHEPLI
                     DHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDIHIDVAFLG
                     APSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVD
                     YVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGAS
                     LAVTRFMREAMIKENIKASFALGGITNAMVELLEEGLVEKILDVQDFDHPSAVSLGKH
                     ADHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
                     DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINPNRQDLVDH
                     FKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVYNV"
BASE COUNT         7115 a         3852 c         4262 g         6969 t
ORIGIN      
        1 tacaattatc gagatcaccg aaaaattgtc gttattgatg gggaggtatc attcactgga
       61 ggtattaatc tagcagatga gtacattaat gaagtagagc gttttggcca ctggaaagat
      121 gctggtttaa tgcttgaggg tgaagcaaca gacagctttt taattttgtt tttacaaatg
      181 tggtctatca cagaaaaaga actgattatt gatccttatc tttcagatca ttctttaaaa
      241 cttccttcag atggctatgt tattccctac ggtgattccc cgcttgatac tgataaaata
      301 ggtaaaaatg tttatataga cattttaaat catgctaaag agtacgttta tatcatgaca
      361 ccttacctta ttttagatag cgagatggaa cacgctttac gatttgcatc agaacgtgga
      421 gtagatatcc gaattattat gccaggagtg ccagataagg gggtaccata tgccttagct
      481 aaaacctatt ataaagcttt gatgacttct ggagtaaaaa tttacgagta tcaaccaggg
      541 ttcgttcatt caaaggtatt tatctctgat aatacaaaag cagttgtcgg aacaattaac
      601 ttagactatc gtagccttta tcatcatttt gaatgcgcga cttatttata tcgtgtgtca
      661 gttattgctg atattgtgaa tgactttaat gaagctcaaa agcaatcact attgatgaca
      721 tcagatcatt tgacgcaacg tccttggtat caaaaattga taggattatt agtacgaaca
      781 attgcccctc tcttgtaatt ttcgtaaaat atgttataat atctgtatgc gatgagtcga
      841 ttgtgactgc aaagtcactt ttgcgcaggg gaggtcatta aggcgggaag ccgactttga
      901 taatctgtgt gaaccatttt atcacaatct tatttattaa gaatgcccct tggattgaga
      961 tattctcaat ccttttttta attttattat cttagttaaa attcagaatg tatcagtgat
     1021 tactgtgttt caaaataaga aagtagtttt tcgggtatga gtgctatgac ttgacaaatg
     1081 ctaataacat ttgcataatg taagtagaga tgaaaactta tgaaaagaga tgatcttatg
     1141 caaattatta agcgtgatgg ccacgttgct gattttgatc cagataaaat ttatcaagca
     1201 attattaaag cagcaaggac ggtctatgtt attgatgaca cgtggcgaca aaacttaaca
     1261 catgtcacta aaaaagtagt tattgattta gaagaagcac atgtagaacg tcctactatc
     1321 aatatggtcc aatcccttgt ggagaatcgt ttgatggatg ctggatatat taccattgca
     1381 gaacattaca tttcttatcg tcttcagcga gatttagagc gcaatggcta tggtgataag
     1441 attgtggttc acttgcgttt tgaacaaata aaataaaaac gcttctaatg gcattttagt
     1501 gtctttgagg ggataaggtt taaataccta acagataaaa atctgttagg tatttttttg
     1561 ctactattag gtgtattcta tatcacatag aacttaagca ttcaagcttt aatttaactt
     1621 tctgtttatt ttttgctaca ttttgggaaa ctcccctcct ttcttagttg aggtgaataa
     1681 acttttattt gataaatcat atttttaagt aatctaaaat tatgtttttt taatttcaaa
     1741 atcatgcttt cgtttgttat aaaaaccgat cttccatagt tagatttttt atccaggcaa
     1801 ttgaagattg gggcaactat tcaattagag attttataat acttatttat ccccattttt
     1861 tatattaatg gtacgaagaa aagttagttg ctctttccac aagtattttt gattagcgag
     1921 tttaatttgc tttgtatttg ttgattgata gcaatagaga gatagcgctt ttttccagtt
     1981 attattattc tatcagattt atctaatgta aagtttctat aagagtctga tgtcgtgtaa
     2041 tcaatcacta aatgagtagg ggttagttga tagtgaatat cagggcaatt ttgttttaaa
     2101 aagagagcca attgacgttc ccgctgtttt agataacgct taataaagcg aagatttttt
     2161 tgaaacatcc cattttctaa ataaagagct aatgcttttt gcattagtaa gttcgtgtct
     2221 aaatcaatca gtgatttttg ggttaaaaaa taaggtttta aacctgacgg taaaactaga
     2281 gctccgatac ggagtgcagg aaaaacactc attgaaaaag attttaggta gatgattcgg
     2341 tgatgagtgt cataataatg aattggtggc tctttaagtt tcacaaaatc tcctaaataa
     2401 tcatcttcta aaatataaac ctggtatcgt tgtgctagac gaacaatagc ttccttttcc
     2461 ttagtgttat aagaaagtcc cagaggatgt gaaaatcgtg aaatcgtgta aaaaaattta
     2521 atatcgccag tttgaaataa agattctaag agttctaaat caaggccatt aaagtgtcta
     2581 gagatagttt gatagggtaa tcctaactga gcaacaatag cttccatccg atgataagta
     2641 ggcttttcaa ggagaattgt tttgcccgca ttaggaaaag gcatttgtga taaaatataa
     2701 agcgcttgtt gcgtcccaga ggtgattaat agttggtctt tgttactgta aacactattt
     2761 tcagctaagt aaagaagcaa agcttccctt agttcttcta gtccttcggt tttgtgataa
     2821 taatgaaaga ggtatttgtc tttagcactt aaagcctcat ttaagcagag tcggaaatct
     2881 tcataagcca tattattata atcttcaaga cttagattaa gaacattatc gggcatgctt
     2941 acgttaccca aaacataata accactttta ggaacagcat aaattaagtg acgatatttt
     3001 aattctaaaa gagcccgctg aacagtatct ttgctacaat gataaacctt acttaatacc
     3061 ctaattgaag gtagcttgtc cccttttttt agtcgctgtt tctggatatc ttgttcgata
     3121 ttggaaatga ttgtttgata ttttgttgtc ataaattgtc cccatacagt taagtgttat
     3181 aactattgta tcttatgcct ctttttctta caatagttat tatagaaaag aggtaatagc
     3241 tatgaaacgg attattgtgg caaatgatct ggttggtgtt ggtaaggtag ctttgtctgc
     3301 tagtattcct ttaatggctt cttgtttgtt agagcaagtt ttattaccaa cttgcctttt
     3361 atcaagccat actggcggag gacagagccc tgtgaagcat actacatcaa gtatattaga
     3421 cgttttttta acagattggg acaacaaaag aattaagttt gacggcatct ttataggtta
     3481 ttttacgaca acaagagatg ggcaaaagct actagaatgg ctttcaagaa agcaacttcc
     3541 cttaatttta gatccaatta tggcagataa tggaaaactt tatcaaggct tcagtcaaaa
     3601 acatatagag attatgataa aacttactca aaaagctgag cttgttttac ctaatattag
     3661 tgaggcttgt ctattaacac aaacagctta tttagcaaaa aattatgatg aagaagatat
     3721 tagacaggtg gctagaaaat tagctaagct tggacctaaa caggttgtca ttagcggaat
     3781 ctcttttgag gagggcagca ttggttttgc ttactatcag tcagattcag atcaattaac
     3841 attccatttt gggaagcaat tcccttacca tttctatggt acaggagata tagcatcagc
     3901 tattataaca gcaggattct attatcagct accactaaca caaattctag atcttgttgt
     3961 ttcatttctt gaaaaagtat tgcaaaatac ccttacatta aaaagagatt taatcttagg
     4021 tgttgcttat gaatctcatt taaattactt aactaaatcg tttaaaaagt tgttggagga
     4081 aaaaaatgtc atctcaaaaa ttagctaatt atattgaatt gagtatgtat gccgctttaa
     4141 tttttatttc agtacaagtg ttgcgtatcc ctatcggtat gcagtttatt catcttggca
     4201 atgcattagt tgttattgca gtcttgatct atggtagtaa aaaaggagct ctagttgcta
     4261 gtcttggttt agggatcttt gatgttttga acggctatgc ttcagtcgtt tggataacta
     4321 tcttagaatc cttggctatt tgtttaattt tgcatttggt atatgaaaga gctatgcaat
     4381 ctagtaaaaa accaaaagcc attataacaa taacagttat tgctgcattg tcaaaattaa
     4441 ctattaacct tataaaatac ttactgttag cttatatagg tactcagcta ccactttcgg
     4501 tatctttttg ggtagctctt ggaaaaattg gtggtagttt cggaacatct tttgtgactt
     4561 tagttgctgt tcctcttttg tatcctgtta tcaaaaactt aaaagaaaag gttacaatcg
     4621 gacaagcaaa aagaggtttt tgatacaagt accttatcga taaaaaagca aggtacactt
     4681 tcagtcaaat attgacaaaa ggttacaatt ttgccataat gaaataacta taaagccgtt
     4741 ttgacggctt ttttgatgac agttaaggaa gaaacgatga gtattttaga agttaagcag
     4801 ttgagtcacg gttttgggga tcgggctatt tttgaaaatg tgtcatttcg cctcttaaaa
     4861 ggtgaacata ttggactagt tggagcaaat ggtgaaggaa aatcaacctt tatgagtata
     4921 gtcacaggac atttacagcc tgatgaagga aaagtagagt ggtcgaagta tgtcactgca
     4981 ggttacctgg atcaacatac agtgttggaa tcaggacaaa ccgttcgtga tgtcttgcga
     5041 actgcttttg atgagttatt taagaccgag aatcgtatta atgagattta cgcgtcaatg
     5101 gcagatgata aagctgatat tgctgttttg atggaagaag taggtgagct tcaagatcgt
     5161 ttagaaagtc gtgatttcta tactttggat gctaagattg atgaagtagc gcgtgcgctt
     5221 ggtgttatgg attttggaat ggagtcagat gttacatcct tatcaggtgg gcaacgaaca
     5281 aaggttttat tagccaaatt actattagaa aaacctgata ttctgctatt agatgaacca
     5341 actaaccatt tggatgctga gcatattgaa tggttaaaac gctatttaca acattatgaa
     5401 aatgcttttg tgttgatttc gcatgatatt tctttcttaa atgatgtgat taatattgtt
     5461 taccatgttg aaaatcaaag tttagttcgc tatactgggg attattacca atttcaagct
     5521 gtttatgaga tgaaacaatc tcaacttgaa gcagcctatg aacgtcaaca aaaagagatt
     5581 gctaacttgc aggattttgt caaccgaaat aaggctcggg tagcgacacg taacatggca
     5641 atgtctcgcc aaaaaaaact tgataagatg gatataattg aacttcaagc tgagaaacca
     5701 aaaccaaatt ttgaatttaa gcaagctaga actcccagtc gattcatttt tcaaacaaaa
     5761 aatcttgtga ttggttatga ttacccattg accaaagaac ccttaaatat aacgtttgaa
     5821 agaaaccaaa aaattgctat tgttggggcc aacggtattg gaaaatctac tttgctaaaa
     5881 agtttattag gtgttatcga gcctttagaa ggtcatattg tcacagggga ttttttagaa
     5941 gttggctact ttgaacaaga agtgacaggt gttaaccgac aaactccgct agaagtagtt
     6001 tgggatgctt ttcctgcctt aaatcaggca gaagttcgag cggcactagc tcgttgcgga
     6061 ctaacatcaa aacatattga aagtcaaatt caagtacttt cgggtggtga acaagcaaaa
     6121 gttcgttttt gtttgttgat gaatcgtgaa aataacgtgc ttattttaga cgaaccaaca
     6181 aatcatcttg atattgatgc taaaaatgag ctcaaacgtg ctttaaaagc atataagggt
     6241 tctattttaa tggtttgtca tgaacctgat ttctacaatg ggtgggtaac cgatacttgg
     6301 gattttagta agttaaccta aactattcct taatttttta cacaggaatt actaaaatca
     6361 gtaaagatat agaataaata ctgctagata tgtgatggtt taggtaactg atcaccaacc
     6421 aaaaaagatg tgactacatc ttttttttat ctaatggaat ggtgtaaagt cgtttaataa
     6481 tagcttattt tttgatgatc agttgttcat gaaattgttg gtgattaaca agtcatttaa
     6541 gatacaaacg ttattactaa aaagtgattc aaatagtttc cctatttttt tatgtttttt
     6601 taaaatttat tacgaataaa tagataaaga cagtgaagat acttagtaag tacatctgct
     6661 aagattacga atagatgggg acaataaatg acgaatttaa cgttttatgc aaaaatcggt
     6721 atttcagaag aagagcatga ctcttttgtt aaagaacatc agcaaattag cgttttacaa
     6781 ggtagtgatt gggcaaaaat caaaaatcaa tggcagaatg agcgaattgg tatctataaa
     6841 gaggaaaagc aggttgcctc tttatcactt ttgattaagc tattgccact tggaagaagc
     6901 attatttata ttccaagagg gccagtcatg gattatcttg accgtgattt ggtggcattt
     6961 accatgaaaa cactaaagga ttatggtaaa actaaaaagg ccctctttat caaatatgat
     7021 ccagctatcc tgttaaaaca atacgcactg ggacaggaag aagaagaaaa acctttagct
     7081 ttagcagcta ttaagaatct ccaagaagct ggtgttcatt ggactggttt aacaatggag
     7141 attgcagata gtatccaacc tcgtttccaa gctaatattt acactcaaga aaaccttgag
     7201 atgcaatttc ctaagcatac cagacgttta ataaaagatg ctaagcagcg tggtgtagaa
     7261 acatatcgtg ccagtcaatc agaacttcac aaattttcca agattgtctc cttaacagaa
     7321 aaacgtaaaa atatttcttt gcgtaacgaa gcttactttc aaaagttgat gactacttat
     7381 ggggataagg cctacttaca tctagcaaaa gtgaatattc ctcaaaaact agatcaatac
     7441 cgccagcaat taaatcttat taaccaagat attactcgca cccaagctca tcaaaagaag
     7501 cgtttaaaaa aattagaaga tcaaaaagct tcttttgaac gttatataac tgaatttgaa
     7561 ggctttacag accaatatcc tgaggaagtt gttgtagcag gtatattatc tatttcttat
     7621 ggaaatgtta tggaaatgct ttatgctggg atgaatgatg attttaagaa gttttatcct
     7681 cagtatctgc tgtatcctaa agtttttcag gatgcttatc aagatggtat tatttgggct
     7741 aacatgggag gagtagaagg ctcgcttgat gatggactta ccaaatttaa ggccaacttt
     7801 gctccgacaa tagaagaatt tataggagaa tttaatctcc ctgtcagccc actttatcat
     7861 attgctaata ccatgtacaa aatacgaaaa cagttaaaga ataaacatta aaagactcgc
     7921 caaaatgagc gagtctttta atgtttattc tgttattccc attcaactgt tgcgggtggt
     7981 ttacttgtga tgtcgtagac gatacggtta acgtggtcaa cttcatttac gatacgtgtt
     8041 gagatttttt tcaagacatc ccaaggaagt tgagcaaagt cagctgtcat accatcaata
     8101 gacgtaatag cacgaatggc gatggtataa tcataagtac gaccatctcc catgacgcct
     8161 acagaacgga cacctgtgtt aactgtaaag tattgccaca cgtcacgatc aagtccagcc
     8221 ttagcaattt cttcacgaag gatagcgtct gattcgcgaa cggtttcaag tttttcttca
     8281 gtaattgctc ccatgacacg gatagcaagt ccaggacctg gaaatggttg gcgccaaaca
     8341 atttcttcag gcattccaag agcgattcca agcgctcgaa cttcatcttt gaaaagagtg
     8401 tttaagggct caatcaattc aaactgcatg tcttcgggga gaccacccac attgtgatgt
     8461 gatttgatgg tttgagcagt ttctgttcct gactcaatga tatcagtata aagtgttcct
     8521 tgggcaagga agtcaacacc ttttaatttg ctggcttcat catcaaaaac atagacaaat
     8581 tcattaccaa taattttacg ttttttctca ggatcttcaa cgtctgcaag aaggtctaag
     8641 aaacgttttg aagcatccac acggataata tttaggccaa atttgccccc aagcattccc
     8701 ataacttgat cgccctcgtc tttacgaaga agaccgtgat caacgaaaat acaagttaat
     8761 tggtcaccga tagctttttg aagtagaaca ccaacaactg aagaatcaac tccaccagaa
     8821 agacctagaa gaactttacg atcgcctaca gtttcacgaa ttttagcaat ttccatgtca
     8881 ataaaattat ccattgacca atcaccacgc gcgccacaaa ttgatatagc aaagttttta
     8941 agaatgtcat ttccataaac agagtgtctc acttctgggt ggaactgaat accgtaaagg
     9001 tttttctcag tattttcaat agctgcatag ggacagtcat ttgagtctcc aacaaggtgg
     9061 aaaccttctg gaatttcagt aacagcatca ccatggctca tcaaaacgag ttgttcttga
     9121 ggtgtgcctg aaaataattt tgacgtttca cgaagatgaa gggttgactg accgtattca
     9181 cgattaccag cttgtccagc aggaacaact ttaccaccta atttatgggt gattaattgc
     9241 ataccgtaac agataccaag aatcggaatc cctagttcaa agatttcagg gtcaatgcca
     9301 aaggcgttat cagcgtaaac agagttaggc cctcctgata aaacgatacc tatgggattg
     9361 atctcacgaa gttcttgagc ggtgatttta tggcttttta gttcggagaa aacaccaaac
     9421 tctcgaatac gtctagcaat aagctgattg tactggctac cataatcaag aacgataatt
     9481 ttttgaacat cattcaaaat tgaaatttca gtcattataa tcctttcatt ctataccaac
     9541 cgtcaggctg atagttgtca acttattgta gcatattttc atcgaaaaga cattacaatt
     9601 tctctagctc gttggaaaaa aacgtaatcg ttatgttaca atagaggcta tgaaagggaa
     9661 ttaagacaac agggagggcc tatgctaaga aaaaagagaa ttttgggttt atttcgacct
     9721 gttgaattaa ttttcctagg attattgttg agtttagttg tttcgtacct tgcgtggacc
     9781 aatagttttg caacacttca caatatatta gcaactgtcg gaatagtgga acgaagcaaa
     9841 gatcaacagc ctcgctatca cattggacaa gctattcaag tacaaaaaag tggtccatat
     9901 catcaatgga ttggaactat taataaacag gtagaagata tagccgaaaa ttaccgagtg
     9961 agttatcatt atgaagtggt atttccaata ggaaaagtca ctgtttcttt gccagaacat
    10021 aacctgaaag agcctgataa accgaggttt aaaaaaggag atatcgttaa attatcttca
    10081 ttaactaaaa agccacatat aaaagtgtat caaggtcaat tagcgactat taaacaagtt
    10141 aaaaaacgct atgactattc gttaggagga tatcagtacg atattaacct gaaagataat
    10201 ctaagattag atggaatttc agagcaagat tttgttaaac cttattatat taggttcaat
    10261 aaaggaaatt cccctgagca aaacaatcgt cttttgcgaa aagctttcgc ttatgcaaag
    10321 cagcatccaa atagcgttat atcttttcca aaggggcaat ttcacattgg ctctttgcct
    10381 tcacaaaaag attattttga gcttccatct gatacagcta ttattggtca tcagacagag
    10441 ttcattattc acggtaaaat gttgtggttt ggattcccta caggaccaaa ggctgaacaa
    10501 ggtgttcgta atctcgtgtt gactggagtg catttcaaag caaatgattt gaaaaaagga
    10561 gaccacttta tgattatggc tgatcatggt actgattggc atatttacga taacaaattt
    10621 actatggttc ataagcgtaa tagtcatatt tttgatttag gatctctaca aaattcattg
    10681 tttgagaaaa accaatttat tggctatgcg ccagaattag tacaagacca acagctgcta
    10741 tcaaaggctc aagggcatga ttttttttca gaagtcattc agtttgatgc tgctgttcat
    10801 cattttgcat gggatggagg tctacttagt aatattgctc caaactatga agcatttaac
    10861 caaactcgac atctatgtca caatattact gtaagccaaa atcaattttt accttatata
    10921 gatccgactg gttgcctgag agcctatagt ggttctattg gtcagcattc ctcaaaagta
    10981 ggagttatta gggttttaaa taatgttttt acctcatcca ttgttactaa agcgaagctc
    11041 actagttggt ttatggaacc tattcatttt ccaccaaatt caccagttat tgtcgcaggt
    11101 aatatcatta attgatgcca aatacctcaa tatttacaat tcaattttca tcaaaaatat
    11161 gataaaatag atgaataacc gacttgagga gtgtggcatg ttaccagctt atataaaaat
    11221 tcatgatgca attaaaaaag acattgatct gggcatctgg ccaattggta gtcgcttacc
    11281 aagtgaacga catttagcgg aacatttcac cgttagtcga atgactctac ggcaggctat
    11341 tacattacta gtagaagaag ggattcttga aaggcggata ggaagtggta cttatgttgc
    11401 cagtcatcgt gttcaagaaa aaatgcgagg aacaacaagt ttcacagaaa ttatacgctc
    11461 ccaaggacga caaccctcct ctaaattatt atcctatcaa aagcaattag ctagtgatac
    11521 cgaagttaaa gaattgaact tagacaaaac agacttggtc attcgaatgg aacgtatccg
    11581 ctatgcagac agtgtccctt tggtgtacga aattgcttca atcccagaaa aatttataaa
    11641 aacggttaaa cgagctgata ttacagaaca cttttttcac tcattgatag caaatggtta
    11701 cgagattggt aaaagtaaac agacaatcta cgctaagcta gcaagtgagc gtgtagcctc
    11761 ctacttggaa gttgctaaag gacatgccat tttggctcta actcaggtgt cttactttac
    11821 agacggcaaa ccttttgagt atgtccgcag tcagtatatc ggtgaccgtt ttgaatttta
    11881 cttagaaaat aattagtaaa gaagtcattt atgacttcct tttttacacg aacagcataa
    11941 ggcaattaat ttgctactgt taaaaacaat catagtatga taaaatgaac attatggaaa
    12001 ttgaaaaaac aaacaggatg aatgcccttt ttgaatttta tgcagctttg ctaacagata
    12061 aacaaatgaa ttatattgag ctgtattatg ccgatgatta tagcttagct gagattgctg
    12121 atgaatttgg tgtcagtcgt caggctgtct atgataatat taagcgtacg gaaaaaattt
    12181 tagagactta tgagatgaaa cttcatatgt attcggatta tgttgttcga agtgaaatct
    12241 ttgacgacat gattgctcac taccctcatg atgagtattt gcaagaaaaa atttccattc
    12301 taaccagtat tgacaataga gagtaagagg agttttgact atggcttttg aaagcttaac
    12361 ccaacgtttg caagatgtgt ttaaacacat tcgcggtaaa aagaaattat cagaatcaga
    12421 tgttcaagaa gtgacaaaag agattcgtct agctctttta gaagccgatg tcgctttacc
    12481 agttgttaaa acctttatta agcgcgtacg tgagcgcgct attggccatg agattattga
    12541 tactcttgat ccaacacaac aaattcttaa gattgttaat gaagaattaa cgagtatttt
    12601 aggctcagaa accgcggaga ttgataaatc tccaaaaatt ccaactatta tcatgatggt
    12661 cggtttgcaa ggggctggta aaaccacttt tgcaggaaaa ctcgctaata aattaattaa
    12721 agaagaaaac gcgcgaccac tgatgattgc tgccgatatt taccgtccag cggccattga
    12781 tcagttaaaa acactggggc aacaaatcaa tgtacctgtt tttgatatgg ggacagacca
    12841 ctcagcagtc gatattgtca gaaaaggttt ggaacaagct cgtgaaaacc gcaatgacta
    12901 tgtcttgatt gatactgcgg gtcgcttgca aattgatgaa aaactgatgg aagagctgcg
    12961 cgatgtcaag gctttagcac aaccaaatga aattcttttg gttgtagata gcatgattgg
    13021 tcaagaagct gccaatgtag cctacgagtt taatcatcag ttgagcatca caggagttgt
    13081 gttaacaaag attgatggtg atacccgtgg aggtgcagca ctatctgtcc gtgaaatcac
    13141 tggaaaacca attaaattta caggtattgg tgaaaaaatc acagatattg aaacatttca
    13201 cccagaccgc atgtccagtc ggattttggg aatgggtgac ttactcactc ttattgaaaa
    13261 agctagccag gaatatgatg agaaaaaatc cttagagtta gctgaaaaaa tgcgtgagaa
    13321 cacctttgat ttcaatgatt tcattgaaca gttagatcaa gtgcaaaata tggggcctat
    13381 ggaagacctc ttgaaaatga ttccaggaat ggctggtaac ccagctttgg ccaatatcaa
    13441 agttgacgaa aaccaaattg ctcgcaaacg tgctattgtc tcttcgatga cccccgctga
    13501 acgtgaaaac cctgacttac taaacccgag tcgtcgccgt cgtattgcag ctggttccgg
    13561 gaacagcttt gtggaagtta ataaatttat caaagacttt aaccaagcca aaagcatgat
    13621 gcaaggggtt atgtcgggcg atatgtctaa gatgatgaaa gatatgggca ttaatcccaa
    13681 taatcttcct aaaaacatgc ctgctggtat gccagatatg tcttccttag aagggatgat
    13741 gggacaaggt ggtatgcccg atttatctgg ccttggtggt gatatggata tgagtcaagt
    13801 atttggtaaa gggttcaagg ggaaaattgg acaatttgcc atgaaacagg ccatgaaacg
    13861 tcaagctaac aaactaaaaa aagcaaagaa aaaacgaaaa taaaagagac ggaaattttc
    13921 gtctcttttt gatgtgaaat tcatttatct tgtatacaag attattaaaa ttatggaaga
    13981 ccgcgttaca tgcatgatga gttcatttaa aatttgaaat aacctaatca atctaactta
    14041 aatttctaag ggcctaaaat tagcgataat tttgccaata atgcgaggtt cttcgctata
    14101 gggagcaaac ttatcagaat attttttatt taaggacact agacgtaatc cctcatcttc
    14161 acgaaatacc tttttgatat atgtttgccc atcccattcg actgcataaa ttgctccatc
    14221 gtaatcaaaa ctattttgtt ttataagaac aacttcgcca tttaaataag ttggctccat
    14281 agaatctcca aaaacccaag acgcaaaatc gtattctaat tgttcatcgt aaaagacaac
    14341 atcaaagtta ccatcaccaa aataggagta accagtacca gcagataaac tttcatagac
    14401 ccgataagca tataattgtg atggtttatc tatgagatct ttagattttt tgtctatttg
    14461 atgatttaac aatcgctctg aatagccaat tactttttct ttattagata ttgtcagctg
    14521 gttataaggc gttagcaatc tatagtttgg gtcgaaataa tcagatgtca cattgaatag
    14581 atgcaataag gcatttaaat gtttttcatt tggtatattc ttaccttttt cccaattaga
    14641 tattgtcatt ttattgacat gcaacaatct tcccaattct gattgagtta ttccctttga
    14701 taatcgtgct gtttttagtt gatgtccaga aaacatttgg tagctccttt agtaaagtta
    14761 taactttact aatagtatca aaaaaataaa acgaaagcaa gtgtaacatt tcctacaatt
    14821 aattcatgtg atagcaatac tgtcaaaaag gggaaacacc taaaacatag cgactagatt
    14881 cgtgtactag caggtctata tctaataaag agttaattac tttaaagata aaattatcat
    14941 ttagttctga gctaaacaaa ttctgtgcct gaagttacta aaataatgat agtaaattat
    15001 tactgaatct aaaagaagtg acgttagtat ctgtaaaaaa agagatttgt ctcgtgacat
    15061 ctttccgaaa aactgtaatt ttcttgaaaa atatttatag gtgtgatata ctgctcttgt
    15121 agagaatgaa tggcataaaa tacatgagac aatttgcgaa aattcatttt tgaaaaaatt
    15181 gttattgtta aggaattatg atgatatgag acgcgaatta ttactagaaa aaattgaaac
    15241 ctacaaggct gtcatgcctt ggtatgtttt agattattac caatccaaat tagctgttcc
    15301 atacagcttt accaccttat atgagtattt aaaagaatac aaacgctttt ttgattggct
    15361 gatggatgct gatttaacgc aggcgccaaa gattgctgat attgacttga gcacgcttga
    15421 gcaccttacc aagaaggatt tagaggcctt tgtgctttat ttgcgggaac gaccttctct
    15481 caacacctat tccaccaaag aggggctttc tcaaaccact attaatcgga ccttgtctgc
    15541 cttatcaagc ctttacaagt acttgactga agaggttgaa aatgaccaag gggagcctta
    15601 tttttatcgg aatgttatga aaaaagtgtc aacaaaaaag aaaaaagaaa ccctggcctc
    15661 cagagcggaa aatattaagc agaaactgtt tttaggtgat gaaactctag ctttcttgga
    15721 ttatgtggat aaggaatatg agcaaaaatt gtctaatcgt gccaaatctt ctttccgtaa
    15781 aaataaggag agggatttgg ctattattgc tctactactt gcttctggtg ttcgtttgtc
    15841 tgaagcagtg aatcttgatt taaaagatgt caatctaaat atgatgatta tcgaagttat
    15901 tcgcaaaggc ggaaaacgtg attcagtcaa tgtagcaggt tttgcgaaag gttatttgga
    15961 gtcttattta gctgttcgtc agagacgtta caaggctgaa aaacaggatc ttgctttctt
    16021 tttaacagaa taccgtggcg ttccaaatcg aatggatgct tctagtatag aaaagatggt
    16081 tggcaagtac tctgaggatt ttaaaattcg tgtgactcct cacaaattgc gtcatacgct
    16141 agcgacaaga ctatatgatg ctactaaatc tcaggtgtta gttagtcatc aattaggcca
    16201 ttcctctact caagtcacgg atctttatac ccatattgtc aatgatgagc aaaaaaatgc
    16261 tttagataac ctttaatgtt acataattgt ttttatgtaa cattaaagat aaaaatagct
    16321 tttaaattaa ctttaataga tgactactat taaccgtgac agtttggtgt caggccgttt
    16381 tccaaactac tcaagccact tagaaaaagg aatattactg ctaactttgt cccttctttg
    16441 aatgactttt ttgtgatgtt tatagattaa atctagtagg gttctcatca aaaagatgag
    16501 ataagcctgt ttttagtatg tcagtacttt tatttagtat gataatacgt tattttgtaa
    16561 agtagctttt aggattattc ttgttaaact acttaatgtc attactgata acttagagta
    16621 atataaagct aaaattgaag gaatattaga gaatcatgaa gacagcttct ttaacatcaa
    16681 ttggttgaaa acaagaatca gataacatta tcgttagata gactgaagaa tcaaaaacac
    16741 agtttgttac ataaataaat tcttgtcaaa atttgtattt tctatttgaa ctgatacgat
    16801 tgacaaaggc taaactaaaa tagcttgact tttttagaat ctttgtgttt tttgtcggct
    16861 cccctcctat tttttccttg aattgatcta tttaaccttt tagaacaagg tttatcgcat
    16921 actattttta gacattttgt tataataaaa agaaacgact tgaggagata aaagatgcca
    16981 aagagaataa gaaaagctct aggagtagtt gggaaactga tgtcaattgt tcctgacact
    17041 actgaaatta ttggaaaaac gattgataac agtcgaccaa ttatcgaaaa acgtatggaa
    17101 caaaaacatg aaaaagagat gcaactacgc acgatagatg atgtgattaa tcttcctgtt
    17161 gatcaggcac aagctcattt ggaacaactt ggctttgtag tagccactat acctgctaag
    17221 cctcataaaa aatggcttca tagtaatctc aatgaagtgg ttgctatgtc tccaaaatct
    17281 ggtaaatata agataggaag cctgatcaaa ctctactaca ttactgttga tgttttggaa
    17341 aaaagtcaag acttattaga ccaagaaaac ttgcggactg tggaacgcaa tcaaaaaatc
    17401 gctgatactt ttgagtctgt caaacacatt cgatttcctt ttaaaaaatg gcgttgataa
    17461 aaaacagagt tgcctttaga tagatcagcc tgttttggta ctaagttatg ttatgattca
    17521 ataaaattaa agatacggag aagaaatgcc ttactacact atttcaaaag tctttccttc
    17581 agataaaacg acaatggcta gtgtaaagaa cctgcttcat caagaaggta ttcgtttgga
    17641 tgctcattta gattatactt gtgccatcat gaatgcgcaa aatgatgtga ttgctaccgg
    17701 ttcttacttt ggaaatagct tgcgctgtct ttgcgtatcc agtgcctatc aaggtggagg
    17761 atttcttaat aggattgtga gtcatctcat tgatgaagaa tatgcccttg gcaactatca
    17821 tctttttgtt tatacgaaga cctcttctgc tgcttttttc aaagatcttg gttttactga
    17881 aattgtccat atcgacaatc acatcagttt cttggaaaat aaaaaaactg gttttcaaga
    17941 ttatctgatg acacttaata agcctgagca aacacctggt aaagtcgctg ctattgttat
    18001 caacgctaac ccctttacct tagggcatca atttttagta gagaaagctg caagagaaaa
    18061 tgattgggtt catctgttta tggtcagtga ggaccaaagc ttggttcctt tttcagtacg
    18121 aaaaatgttg attcaagaag gattgaaaca tcttgataac attatctttc atgaaacagg
    18181 tccttatttg attagtcagg caacctttcc agcttatttc caaacggaag acaatgatgt
    18241 gatcaaaagc caagctttgc tggatactgc tatttttcta aagattgctc aaaccttaca
    18301 gattacaaaa agatacgttg gagaagagcc aaccagccga gtaactgcta tttacaatga
    18361 aatcatggca gagcagttgc agcaagctgg tatcctccta gatattttgc caaggaaagc
    18421 tatcaatcag cagcaagatc ctatcagtgc ctcaacggct aggcaggcat tgaaagatag
    18481 tgattgggac cttctggcaa aactccttcc caaaacgtct ttagattatt tttgttcgct
    18541 agaagcccaa cccattatta aaaagataca agccacttca tctgtcaaac attactaaga
    18601 taaaaaacct atctgattat caaaatcagg taggtttttg gtattgttct tacagcaaat
    18661 gcgtttcacc aaggagatta atggttaaga ggattagtca ttccctgtta gtcttgaatg
    18721 tccagttgta cagtaacttc ttgaaccgga acatcataga aaggatcttc acggcgtccc
    18781 aagaaatctc tggcttgctg tgggaaactc gcatagctta acacgtcttc ttctgaacga
    18841 gcgtattgag caatctcgtc acgtagataa atcatttgag gctcgattaa gtcagctggt
    18901 ctgcaagcaa tgactgcttc gtcaccaatg attttctctt tgataccttc tgctaatggg
    18961 gctggtgatt gaccgtatag ccctcgaaca tagtctttga tttcatttgg aactaccttg
    19021 tagcgttccc ctgagatgat attcatcaag gcctgtgttc caaccatttg tgataatggc
    19081 gttacgagtg gcggataccc aagatcagcc cttactttag gtacttctgc taagacttct
    19141 tcgtatttat cagctaggcc ttgctcggtt aattggctca ataggtttga tagcatgcca
    19201 cctggaactt ggtaaatcaa tgtttttggc tcagtatctt tgactttagg atttaaaatc
    19261 cctatttctc gatagtggtc acgaatagtg ttgaaatatt ctgcaacttt ggccacctct
    19321 tgcatatcca agcctgtgtc aaagcctaaa tccgtcaagg caatcgccat tgactctgtg
    19381 gcaggctgac tggttccccc tgaaaaagag gaaatcgccg tatcaataat atcagctcct
    19441 gcttctgcta cttttaagta agtcatttct gaaataccac tggtagcatg ggtatgaacc
    19501 tcaagaggaa tagttgtatt ctccttgata catttgacca attggtaacc tatttcagga
    19561 gttaagacac cagccatgtc tttgatacaa atggagtccg ctccgatagc ttggtaagct
    19621 ttcgccaatt caacaaagta gtcaatggta tgtaccggac ttgtcgtgta agaaatggca
    19681 acttgagcat gccctccaaa ttttttagtc gctgaaacag ctgtttgcaa gttgcgaggg
    19741 tcatttaaag catcgaagat acggacaata tcaatcccgt tttcaatgga tttttgaacg
    19801 aaggatctga ccacatcgtc agcataattg cgatatccaa gaaggttttg tccgcgtaag
    19861 agcatctgaa gtttagtctt tttcactgct ttacggatgg ctcttagccg ttcccaaggg
    19921 tcttcattta aaaagcgtag gcaggaatca aaggttgctc ctccccacat ttcaagggca
    19981 tgataaccag cattgtctag cgtttcgaga ataggaatca tttccttggt tgtcatacgt
    20041 gtggcaatct gactttgttg accatcacgt aaaactgttt ccgttatacg gattttcgtc
    20101 ataattgttc tcctcatcaa cctgtcttat ctgatgacga tgattggttg tctttattga
    20161 aaaatgaatg gagtaatttt gaaatagcag tctgcatctc ctcgacactg tgcttacgta
    20221 ggcgaccgca gactttggcc tctttagaac agataaaaca ttgcctaggg gagcctccta
    20281 atacggttct gctaattgaa tgaggcagat cattttgcaa gaccaagaca tctaaatcca
    20341 tgagacgacc tattggcaac tctgtctcta aggctatcat ttttaacttc aggtcgcggc
    20401 taggtagact tgtgatgagg taatactcat aacccgttgt aggcagtaat cgcaactggt
    20461 aacaaatctt atcgtcagct aaggcagttt gaatggcctt aatcactata tcaaaaactt
    20521 caagaagttt aggagaggtt ttaattggtc cagggatatt catggtgacc gataataagc
    20581 taccttctgg gtactccttt aataaatgca gctgacgcag agctctttct tcgcgggctc
    20641 ttaacatatc actaagttgg atagcttcgc ctgaaaaata agtgtcttta cacattgtaa
    20701 accacatcca tgaggctacc atcacggtat tcaataagag caacaacctt atcaccatat
    20761 tgaatacgct caggtgtgcc aacgatagcg taagccttct cttttagctc ctcaatacta
    20821 aattgtggca cattgaggga tttgaaatgg tctactaagt cttgacgatt tgggttaatg
    20881 gcaattccca cttctgtgac gaccacatcg acacttgttc ctggtgtaat gaccgtatta
    20941 acctcatcta caaaagttgg gatgcgtccg cggataagtg gtgaaataac caaactcatc
    21001 ttagccgcaa aggcagtgtc gcagtgtcct ccagaagcgc cacgaatcac gccgtcagat
    21061 cctgtcatca cattaacatt gaagttagta tcgacttcaa gggctgataa aatacaagtg
    21121 tctaattgat tgataacagc acccttgctc aaaggtgagg catacatgtt ggcatcaatt
    21181 tcataatggt cagcatgctt accaagagaa acagctgatg ggtgatcaaa gtcttggaca
    21241 tcaagaattt tttcaaccag tccttcttcg agcaactcca ccatagcatt ggtaatacca
    21301 ccaagagcaa agctagcttt gatattttct ttaatcatag cttcacgcat gaaacgggtt
    21361 actgccaaag aagcgccacc tgttccagtt tggaaagaga atccttcttt aaaataagga
    21421 gagttggtga ttactttagc ggcgtattct gcaattaaga gttctttagg atttttcgta
    21481 aaacgagttg cccctttggc gattccttga ggatctccga tagcatccac tgttaccaca
    21541 tagtcaacat ctgtttgagg aatgctgatt ggggtattag gataaggaac caaattatct
    21601 gtcaagataa ccacctgatc cgcatatttg gcatcaatca tggcatagcc caaagaacca
    21661 caggttgctt tcccttttgt cccattaaca tttccgtaag catctgaact tggggcgccc
    21721 agaaaggcaa catcaatatg gatatcccca ctagcaatgg cacgagcgcg accaccatgg
    21781 gagcgaataa ccacaggatt ttccattagt ccttctgaga tggctgcccc tactttgtca
    21841 cgaagaccag atgaagtgat gttagtgacc acaccatttt tgatgtgatc aatcaaaggc
    21901 tcatgtacat tagcgatgga acttggggca atagacaggt ttttaatgcc cattttggca
    21961 atttcctcca agaccatatt cataatgaaa tccccttcac ggaaatggtg gtggaaagaa
    22021 atggtcatgc catctgttaa acctgttttt tcaatcgctt cacgaacaga tcctagtaat
    22081 ttagaatctc caggcttaac tggtttaata cggcgacttg attcgtcata ctgtttgata
    22141 tttgcgagtt ctccttcaaa gacaccgtat tggtcggcgt atggttgagg aatatctc
//
LOCUS       DAUVUY010000022        21872 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.022, whole genome shotgun sequence.
ACCESSION   DAUVUY010000022 DAUVUY010000000
VERSION     DAUVUY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21872
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.022"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..2485)
                     /locus_tag="VOX58_000754"
     CDS             complement(<1..2485)
                     /locus_tag="VOX58_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HES5878001.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLF"
     gene            complement(2715..3101)
                     /locus_tag="VOX58_000755"
     CDS             complement(2715..3101)
                     /locus_tag="VOX58_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HES5878002.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            3183..4484
                     /locus_tag="VOX58_000756"
     CDS             3183..4484
                     /locus_tag="VOX58_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HES5878003.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEVLFHTDHEAFTQEIITNPETEINAILVRHALDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSRGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMIQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            4940..5749
                     /gene="yidA"
                     /locus_tag="VOX58_000757"
     CDS             4940..5749
                     /gene="yidA"
                     /locus_tag="VOX58_000757"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HES5878004.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHMVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            5749..6975
                     /locus_tag="VOX58_000758"
     CDS             5749..6975
                     /locus_tag="VOX58_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HES5878005.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWRKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGIEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLDIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            6962..8197
                     /locus_tag="VOX58_000759"
     CDS             6962..8197
                     /locus_tag="VOX58_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HES5878006.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQNDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(8296..9054)
                     /locus_tag="VOX58_000760"
     CDS             complement(8296..9054)
                     /locus_tag="VOX58_000760"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HES5878007.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(9295..10491)
                     /gene="tuf"
                     /locus_tag="VOX58_000761"
     CDS             complement(9295..10491)
                     /gene="tuf"
                     /locus_tag="VOX58_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HES5878008.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            complement(10845..12149)
                     /locus_tag="VOX58_000762"
     CDS             complement(10845..12149)
                     /locus_tag="VOX58_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HES5878009.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
     gene            complement(12289..15087)
                     /gene="ppc"
                     /locus_tag="VOX58_000763"
     CDS             complement(12289..15087)
                     /gene="ppc"
                     /locus_tag="VOX58_000763"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HES5878010.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQAIIAEEVALLKEMLENITRR
                     MIGDDAFAVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINYQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQIQLTNQPAARMSSSVGLSTSAWSSPASLENPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQESCVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     GNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            15246..17045
                     /gene="pepF"
                     /locus_tag="VOX58_000764"
     CDS             15246..17045
                     /gene="pepF"
                     /locus_tag="VOX58_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HES5878011.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALQDIDLFKRN
                     YEGRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVSYENFYQNHENAEIRDKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYASVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIINYTEEMSHA"
     gene            17038..17517
                     /locus_tag="VOX58_000765"
     CDS             17038..17517
                     /locus_tag="VOX58_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HES5878012.1"
                     /translation="MPNLYDFTVKAQNGNDLSLAAYKKKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFTWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(17564..17947)
                     /locus_tag="VOX58_000766"
     CDS             complement(17564..17947)
                     /locus_tag="VOX58_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HES5878013.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQTQTPSVPKADHHVRLVVK
                     EDTNTIDEKVSFGKGDTVLEILKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNGK
                     MADKGADQITVKDGDSIEFYQEVFK"
     gene            complement(17931..18434)
                     /locus_tag="VOX58_000767"
     CDS             complement(17931..18434)
                     /locus_tag="VOX58_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HES5878014.1"
                     /translation="MSAKFMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFALYPLSQQFPKYQLGYQ
                     AFLAALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            18759..19607
                     /locus_tag="VOX58_000768"
     CDS             18759..19607
                     /locus_tag="VOX58_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HES5878015.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKTSTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     regulatory      19735..19831
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            19876..20439
                     /gene="thiT"
                     /locus_tag="VOX58_000769"
     CDS             19876..20439
                     /gene="thiT"
                     /locus_tag="VOX58_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HES5878016.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMALSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     gene            20529..21008
                     /locus_tag="VOX58_000770"
     CDS             20529..21008
                     /locus_tag="VOX58_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HES5878017.1"
                     /translation="MAKKTKFKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            21349..>21872
                     /locus_tag="VOX58_000771"
     CDS             21349..>21872
                     /locus_tag="VOX58_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HES5878018.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKSDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLA"
BASE COUNT         6816 a         4520 c         4004 g         6532 t
ORIGIN      
        1 tgaacaattt gtttttgaac aacccaacag taaagatgga ttggtaaaca gatttcacgt
       61 tgtaggcatg taccaactga attaaaccga gggtcacata agccatggtc aaggcatctg
      121 catggacatc atggtagctt gtgtgttctg ggaacatcaa ggcaaaacca taaaccccaa
      181 gcaccaaaat ggtttggaag gcaccttggt aaagaatggc ttccttcacc ccaccatcaa
      241 agaagcttga cttacgtcca cgaggtttgt gtttcatcac accaggctca gcaggttcaa
      301 caccaagagc aatagctggt agggtatcag ttaccaagtt aatccaaagc aagtgaactg
      361 gttgaagcac atcccaacca aacaaggttg caaggaagat ggtaaagact tctgccatgt
      421 tagcagaaag taaatattga attgtttttt gaatatttga gaagacctta cgtccttctt
      481 caaccgcaac gataatggtt gcaaagttat catcggcaag aaccatgtca gaagctccct
      541 tagaaacctc tgtacctgta attcccatac caataccaat atctgccgtt ttaagagatg
      601 gtgcatcgtt aaccccatcg ccagtcatgg caacaacttt gccttcattt tgccaagcct
      661 taacgatacg aactttatgc tcaggagata cacgggcata cacggagtat tgtttaaaga
      721 ctttttggaa ctcttcatct gataattcgt tgagttcagc acctgtaaag acatgatcaa
      781 cgccatcttc ttcgataata ccaagacgtt tcgcaatagc ttttgctgta tcttggtggt
      841 caccagtaat catgattgga cgaatgccag cttccttagc tactttaaca gcctgtgctg
      901 cttcaggacg ttcagggtca atcatcccta ccaaaccaga gaagacaaga ttagcctcaa
      961 caatttctgt ttccagtgtt ggaagagcat cagaatattt gtaagccatc atcaagacac
     1021 gaagagcttg tttggcaaga ctcttattgg tatcaagaat ggttttttta tcagcatccg
     1081 taataggacg aatttgtcca ttttcttcaa tctgtgtgac acgcttcaac aattggtctg
     1141 gcgcaccttt aacagcgatg aaatatttgc catcagcttg tttatgaatg gttgacatca
     1201 acttacggtc agaatcaaat ggtaattctg ccacacgagg ttctgccacc atagcctcac
     1261 gtacgtcaaa attgtggtct aacccaaact gtaccaaagc tgtttctgtt gggtcaccaa
     1321 ttaacttccc tgaaggatca actttagtat cattagcaaa gttcattaca cgaagtgtcg
     1381 tattgtcaaa agcaatatca gctgatgaac tttggagtgt cccgttggta taaacttttt
     1441 caacggtcat ttgattcatt gtcaaggtac ccgttttatc agaagcaatg atttcagttg
     1501 atcctagcgt ttcaacagct ggtaatttac ggataatggc gttacgttta gcaagtactt
     1561 gagttccaag agaaagcaca acagtaacaa tcgcaggaag tccctctgga atagctgcaa
     1621 cggcaagcgc tactgaagtc atcaagcctt caagcgggtg ttgaccacgc aagaagactc
     1681 caacagcaaa agtcaccgct gcaatcacaa ggatcgcata agtcaaaatc ttagaaaggt
     1741 tgtcaaggtt ttgcttcaat ggtgtatctg tctcatcagc attagcaagc ataccagcaa
     1801 tatgcccaac ctcggtgtac atgccagtgt tagtgataac cccaatccca cgaccatatg
     1861 tcacatttga gttttgatat cccatattga cgcgatcacc aataccggca tcttctgaaa
     1921 cagccgttga caagtctttt tcaaccggta cagattctcc agtaagcgct gcttcttcga
     1981 tttttaagga gttagcttcc aaaagacgca gatcagcagg gacaacatct cccgcttcta
     2041 gcaagacaat atctccaggg accaattctt tagagtctat ctcagtcaca tgcccatcac
     2101 ggcgaatacg agccagtgga cttgacattg atttcaaggc ttcaatagca gcttcagctt
     2161 gtccttcttg gtaaacacca aaagcagcat ttaggataac aacagccaag ataataatgg
     2221 cgtctgtcaa accttccatc ccctctgtaa gaactgaaag aagggctgcc acaatcaaaa
     2281 taataatcat caaatcctta aattgatcaa ggaatttcat aaataaactt cgtttttcgc
     2341 cttcatcaag ctcattacga ccatactctg ctaagcgctc ttttgcttgg gcagacgtaa
     2401 gaccttcgcg actggtctct aattgagcca gtacggtctc ttcactttgc gtgtaaaatg
     2461 cttcatgtct ttgttcttta gacaaattag tacctccttg gtctttttat tttagttgtt
     2521 caaataaaaa gacctgttta ttttcttaaa caagtctcgc tgtttaagat tgtaccggaa
     2581 accgaaattc cgtattgacg atacaatcac ggctagccgt ctgctactcc cttgatataa
     2641 taaccattat agtaaaaacc tgactaaaaa acaagaataa tccctaatca gatgatttac
     2701 tgctatcttt ttagttaatc ccaactcagc tcaagatgat agcttgcaaa tagcatttct
     2761 gtttgagctt gaagcaaatg ataatgaagg tccaattttt gacaagaaca atctaagtgg
     2821 taatgactgg tatctgtcaa aaattgttga acccccatcg gtgtaggtag agcaatcagg
     2881 accttcttgc ccgcaaaaaa tttcatgatt gactgagggt tggaaaaacg gctcatggtc
     2941 agttctgtct cattatactt gatgacaacc ttttccttgt cagtgttttg atagatgagg
     3001 tactgatagc cccctttttc tcgccactca caatcatgaa tttcttgaat aatctctgtc
     3061 tcatctccaa agcgaatatg gttggttaac tgtaatttca taactcttct ttctattcta
     3121 agctcgtctg tttattctat caatttctcc ctattttatc aatttttgat ataataaaaa
     3181 ttatgaatga aaaagtattt cgtgaccctg ttcacaacta tatccacatt gataatccat
     3241 taatttatga ccttatcaat accaaagaat tccaacgctt acgacgcatc aaacaagtcc
     3301 ctacaacagc tttcaccttc cacggcgcag aacatagccg tttttctcac tgcctaggtg
     3361 tttacgaaat tgcccgtcgg gtgacagcta tttttgaaga gaaatacgct gatatctgga
     3421 ataaagatga aagtctggtc actatgacgg ctgccctctt acatgacatt ggtcatggag
     3481 cctattctca tacctttgag gttctttttc atacggatca cgaggccttt actcaggaga
     3541 taattaccaa tcctgagaca gaaatcaatg ccattttggt gcgtcatgct cttgattttc
     3601 cagacaaagt tgccagcgtg attaatcata cctatcctaa caagcaagtt gtgcaactga
     3661 tttccagcca gattgactgc gaccgtatgg attacctttt aagagattct tatttttctg
     3721 ctgccaatta tggtcagttt gatttaatgc gcattctacg tgtgattcga cctgttgaag
     3781 atggcattgt ctttgaacat agtggtatgc atgctgtcga ggactatatt gtcagtcgtt
     3841 ttcaaatgta catgcaggtc tattttcatc ccgccagccg tgctgttgag ctcatcttac
     3901 aaaatctcct caaaagagcc caacacttat atcctgagca acaggcttat ttccaaaaaa
     3961 cagctcctgg actcatcccc ttctttgaaa agaaggctaa tttagcagat tatatagccc
     4021 tagatgacgg agtcatgaat acttacttcc aagtctggat ggcaagcgaa gaccatatct
     4081 tatcagattt ggccagtcgc tttattaatc gaaaaattct gaagtctgtt acctttgacc
     4141 aagattcccg aggagagtta gaacgcttgc gccaattggt cgaatcagtt ggctttgatc
     4201 cagattatta caccggtatc catatcaact tcgacctgcc ttatgatatt tatcgaccag
     4261 agctcgaaaa tccaagaact cagattgaaa tgatacaaaa agacgggagc cttgctgaat
     4321 tatctcagtt atctcctatt gtcaaagcat taacagggac aacctacgga gaccgtcgct
     4381 tttattttcc aaaggaaatg ttagagctcg atgacctctt tgctcctagc aaggaaactt
     4441 ttatgagtta tatttccaat gggcattttc actttagtca gtaaagccct ttaagattgc
     4501 agaaaaagtc ttttttgata cttttcttag gtgatgctta tggcagcgac ttcctttgga
     4561 attctatgct ttatttttga gcctagggtc tcaaaaattc cgcgtaacgt cactgccagt
     4621 attgacacca cttttgccac agcaaaaagc agctgttttg gctcacgtca cacgcagaaa
     4681 taagaaatca gttgtcaaat cagcagagca gttcatgctt tttgagcatc aaactgggtt
     4741 tgtctccatt ctaagattga agaaagagta ctttcttcaa tctttttagt tactacagag
     4801 gcgattgagc taaactaaaa cgttttctct cgtttttcct taagtttcct ttctcatccc
     4861 atgacaaaag cacctatttt tagtaagata ggagggagaa catttttaag ataaaccaca
     4921 aaaaaacaag gaggatgcca tgtctatcaa actggttgct gtcgatattg atggcacatt
     4981 actcactgat gaccgtcgaa ttacagatga tgtttttcaa gctgtccaag aggctaaagc
     5041 ccaaggggtt catatggtga ttgctactgg cagacctatt gccggagtga tttcattact
     5101 cgaacaatta gaactcaacc acaaaggaaa ccacgttatc acttttaatg gtggcttggt
     5161 gcaagatgcc gagaccggcg aagaaattgt taaagaattg atgacttacg acgattatct
     5221 ggaaacggaa tttttgagcc gaaaattagg agttcacatg catgccatta ccaaagaagg
     5281 tatttacact gccaatcgga atattggaaa atacacagtt catgaatcga ctttggtaaa
     5341 catgcctatt ttctatcgca ctcccgaaga aatgaccaat aaagaaatca tcaaaatgat
     5401 gatgattgat gaacctgacc ttttggacgc ggccatcaag caaattcctc aacatttctt
     5461 tgataagtac accatcgtca agtcaacccc tttttattta gagttcatgc caaaaaccgt
     5521 cagcaagggc aatgccatca aacacttagc taaaaaactc gggctagata tgtcacaaac
     5581 catggccatc ggggatgctg aaaatgatcg tgctatgtta gaagtcgttg ccaaccctgt
     5641 tgtcatggaa aatggggttc ctgaattgaa aaaaatagcc aaatacatca ccaaatccaa
     5701 caatgacagt ggtgtggcac atgctattcg caagtgggta ttaaactaat gtatacatat
     5761 aaaatcggaa tatctcctga agaacatgat cagtttgtct tagcacagcc acaagctggc
     5821 ttacttcaaa gttcaaagtg gagaaaagtc aaagataatt ggaaacacga acgcatcagc
     5881 ttttatgaaa atggggtaca agttgctgct gctgcttgtt taatcagaaa acttcctctt
     5941 ggtttcacta tgatttatat cccacgtggg ccaatcatgg actacgccaa ttttgaactc
     6001 cttgatttcg tcattaaaac gctcaaaact ttcggaaaaa gcaaacgagc gctttttgtc
     6061 aaaattgatc caagtttagt catcaaacaa actttagaag gaaaagaaag caaagaaaat
     6121 gatgtgacac tctctattat tgcttttcta aaaaaacttg gtatcgaatg gtctggcaga
     6181 acaaaagaac tcgaagacac tattcaacca agaatacaag ccaacatcta cgccaaggat
     6241 tttgactttg acagcctgcc caaaaaagcc aagcaatcca ttcgtaccgc taccaataaa
     6301 ggtgttaatg tcacgattgg tggcagtgaa ttgctagacg acttttcagc cttgatgaaa
     6361 aaaaccgaga atcgaaaagg cattatcctt cgaggcaaat cctactatca aaaattacta
     6421 gatatttatg ctggtcagtc ctacattact atggctagtt tagacttacc tgaacaaaaa
     6481 aaactcttga tccaacagtt ggacaaggcc ttagcagaac aagcacgatt aacagataag
     6541 agtaaaccta gcaaagtagc agaaaatcaa aaaaccattg cacggctaca aaaagattta
     6601 accattttaa gcgagcagct cgctgcaggc aaaacccgca tcccactagc cgccacccta
     6661 accttgattt atggcgagac ttctgaaaat ctttatgcgg gaatggacga tgattatcgc
     6721 aattatcaag ctcctctttt aacctggtat gaaacggcta aagaagcttt taaaagaggc
     6781 tgccgctggc ataatttagg aggtgtggaa aatcaacaag atggtggact ctatcatttc
     6841 aaagctagac tcaaccccac catcgaagaa tttgcaggtg aattcaacat tcctgtcggc
     6901 cttgtttcaa gcttagccat cttgacctat aacttaagaa aaaaattaag gagcacaact
     6961 aatggcacta attgaaattt cacaagaaca atttgaccat tactgccaca gtctcgtcca
     7021 tcattccttt atccaaacct cagaaatggc cagcttgatg gctaaaagag gggcaaaacc
     7081 acaatttctt ggacttgaaa aagatggtga actaaaggtt gctgctatgg tctttagcca
     7141 aaaagtggct ggaggttggc gtatggaact caatgcaggc cctaatacca atcatcccga
     7201 agaattagaa catttttaca ctcaacttaa agactatgcc aaacaaaatg acgttatcga
     7261 actcatttta aaaccttacg acaattatca gtcatttgat actgatggca tccctatttc
     7321 tcatccaaac actgacctta ttagcctttt aacggcgtta ggatataaac atgacggttt
     7381 aaaaacaggc tatcctgagg gtgaacctgt ttggcattac gttaaaaagt tagaaggaat
     7441 tgattcctca cgtttaaccc attccttcag caaaaaagga aaagctttaa taaaaaaagc
     7501 taatactttt ggtattaaat tacgtcaact gaaacgagat gagttacacc atttcaaaga
     7561 aatcactgaa gcaacctctg atcgtagaga ttaccttgac aaatcattat cttactatca
     7621 ggacttttat gatagttttg gagactcttg tgaatttatg gttgcgactc ttaattttga
     7681 agattacctt aataacctta aacaaagaca actacaacta gcaaccagca tcaacaaagt
     7741 taaaggagac cttggcaaaa atcctcattc tgaaaaaaag caaaatagat taaaagaact
     7801 atccagccaa ttcgaaacct ttcaggtaag aatttctgaa gctctccatt ttcttgaaga
     7861 gtacggaact aaagatgtgt tccttgcggg aagtcttttt atctataccg aacaagaggc
     7921 cgtctacctc ttttcaggat cttaccccaa atttaataaa ttttatagcc cagctctatt
     7981 acaagagcat gctatgctca aggcaattca caagggcatc aaacaatata attttcttgg
     8041 tattactgga aagtttgatg gttcagacgg tgtcttgcgc tttaaacaga atttcaatgg
     8101 ctttattttg caaaaaccag ggacatttag atgttatcca tttccaatca aatatcattt
     8161 cattagattg gcaaaaaagc tattaaaccg ctattaggct aatgcatttg cctgtagaat
     8221 cacctttatt ctctcatagt aagagttaag tcatctaaaa aagaaaagga tttaaccttt
     8281 tcttttttag atgacttagt taaggaaatc aagcaaagca aggaagctat cagcttcaag
     8341 tgatgcacca cctacaaggg caccgtcaac atctggacaa gccatgtaat ctttaacgtt
     8401 ttcaggtttt acagaaccac cgtattgaac gcgaacttta tcagcaactt cttgcccaaa
     8461 gtctgctgct accacgtcac gaacagcttt acacatgttt tgagcatcat cttgagtagc
     8521 tgatttacca gtaccgatag cccagattgg ctcataagct aaaacaagtg aagctacttg
     8581 ctctgctgaa agacctgcaa gtgctgctga aacttgcgca cctacaaact caacggcttt
     8641 accagcttca taagtttcaa gagactcacc acaacaaaca attggagtta aaccgttagc
     8701 aaagattgct ttggcttttt tattaatgtc ttcatctgtt tcatggaagt aatcacggcg
     8761 ttctgagtga ccaatcacaa catagtcagc tcccatttca gcaagcactt ttggtgatgt
     8821 ttcaccagtg aatgcccctg tgttttcaaa gtagcagttt tgtgcagcaa ctttaagaac
     8881 tgaatcttta gcagcttcaa tagtggttac caaatcaaca gctggggctg ctacggcaac
     8941 atcaacaagg tcagttgaag gtaatttgct tgctacagcc tcaacgaatg cttttgcttc
     9001 ttgagggttt ttgttcattt tccagttacc agcaataatt ggtttacgtg acatatcaca
     9061 tacctcttct ttttttattt atatcttcta ttttagcaca aatgaccgac gattaaaagg
     9121 aattgcttat attttaggaa tattttcaca aataaaaacc ctttttctga aagcttttca
     9181 ttcttattta tcagcatttt atggagttta agatagcaaa aagcccccga attgggggct
     9241 tcttcaaact taagttgtct gacttaaatt gctattctgg gaacttaatt aaaattaagc
     9301 ttcgatttct gaaacgatac ctgaaccaac agtacgtcca ccttcacgga ttgagaaagt
     9361 agtaccttgt tctacggcga ttgggtggat caactcaacg ttgattgtca cgttatcacc
     9421 aggcataacc atttctgtac ctgctggaag ttcgattgaa cctgttacgt cagttgtacg
     9481 gaagtagaat tgtggacggt agttgttgaa gaatggagtg tgacgtccac cttcgtcttt
     9541 agaaaggata tatacttcac ctttgaattt agtgtgtggg ttgattgaac ctggtttagc
     9601 aataacttga ccacgttcga tttcgtcacg ttgaacacca cgaagaagga tacctacgtt
     9661 gtctcctgca agaccttcgt caagttgttt acggaacatt tcaacaccag taacaacagc
     9721 ttttttagtt tcttctttga taccaacgat ttcgatttcg tcgttgacac gaacagtacc
     9781 acggtcgata cgtcctgaag caactgtacc acgacctgtg attgagaata catcttcgac
     9841 tggaagaagc aatggtttgt cagtgtcgcg ttctggttct ggaatgtatg aatcaacagt
     9901 atccatcaat tccatgatga tgtcttcaaa tttagtgtcg ccttcaagag ctttaagagc
     9961 tgaaccttgg ataactggaa ggtcatcacc tgggaaatcg tattctgaaa gaaggtcacg
    10021 aatttccatc tcaactaatt cgagcaactc ttcgtcatca acaaggtcaa ctttgttcat
    10081 gaacacgata aggtgtttaa caccaacctg acgtgaaaga aggatgtgct cacgcgtttg
    10141 tggcattggt ccatctgttg aagcaactac aaggatagct ccgtccattt gagcggcacc
    10201 agtgatcatg tttttaacgt agtccgcgtg tcctggagcg tcgatgtgcg catagtgacg
    10261 agttgcagtt tcgtactcaa cgtgtgcagt gttgatagtg attccgcgtt cgcgttcttc
    10321 tggagcagca tcgatagaag cgtaatcttt tggttggtta actgatgaag gcaagcgacg
    10381 tgccaataca gttgtgattg cagctgttaa agtagtttta ccatggtcaa cgtgtccgat
    10441 tgtaccaatg ttaacgtggg gtttactacg atcgtatttt tcttttgcca ttttaggaaa
    10501 agcctccaat aaaatatatt ttatagatag acggtaggcc atacagtcta actttacctt
    10561 actattgtag caaattacaa tgaaaatgca agtattttat gttttttgcc atttctttat
    10621 tttttaaata gaagaaaaag tcttttttga tactctcctt aggtggtact tatagcagcg
    10681 acttcctttg gaattccaca ccttattttt gccacagcaa aaagcagctg tttgggctca
    10741 tgtcacacgc aaaaataaga aatcagcaga gcagttcaag ttttttgagc atcaaactgg
    10801 gattgtctat attctaaaat ctccttaaag gagattttag aatattattg aaattgcttg
    10861 atatttacaa ctttactatt ttcttttcga gtgtcctttc gataagataa ttcagcttct
    10921 ttaaatatat catctcgctt ttcgctagca tcaatattta gtacaaaacc cactgcaaca
    10981 gataagacaa gtaagctatt tccaccttga gacaaaaaag ggaaggtaac accagtagat
    11041 ggaattaagc ctgagatacc accaatatta acaaagactt gcatgagcat cataccgcct
    11101 actcctaaag ccatcatggc attaaaagga ttttttgctt taataccaac attcataata
    11161 cgtaaaatta agaagaaaac aagtgccaaa ataaaccctg ctcctattag acctaactct
    11221 tcaatgacaa ctgaaaagac aaagtctgtc tgtgcttcgg gcaaatagcc acgcttttca
    11281 atggaatttc ctaacccttg gccaaaccac ccaccattac tcatagcata gtaagaattg
    11341 gctaattggt gacctgaatc ggtcaagtca tgaaaaggat taaaaaaggc actaaaacgt
    11401 ttagcgacat agccaaaaac aggaatctta gcaactcttt ctaccccaat aacggcaata
    11461 gtcccaagaa agaccgtaga tagaccggta atcatgacta ggatagccga aaaccatcgg
    11521 taaccaatgc cactgataga aaacataata atggctgtca atacaataat ggaagcattt
    11581 cctaagtcag gctgagcagc aaccaaaagt accatcaaca aagaataaac acgccagtcc
    11641 cgtaaatcgt tccattgtgt tggccaccat tttcgacgag ttagtgcttg ataatcataa
    11701 aggctaatgt ttttttggat cttagcaaag gtcaatgcca aataccatac cataatgatc
    11761 tttaaatact cagcaggctg aaagctgact ggtccgatca caatccaacc atgcgctcct
    11821 ttgatagcag ttgtaaagaa gcgggcaata attaaaagaa aggcctctcc taacataacc
    11881 acagttaata cccttgtatt cgttaaaaaa tttaatttta atttatagat aaatgtaata
    11941 gccactagac ttattatcca aaaaactccc tggttaatca ccgatttaaa tggattagca
    12001 tgtgcttgaa tcaagctgac acttgtggtt gagtaaacca ttattaagcc tatcaccgat
    12061 aaaattaaat aaggcaataa tatagaataa tttaagaggt gccttttatc aatcttcata
    12121 gttttccctc tacttcttga gataatcatt tagcattata acatctttct atatcaaaca
    12181 aaagaaaaag tcaagaatcc tcaaaaaaag aagctccaag ggctaacctt taaaattctg
    12241 attagtcact tgaaaacttc tataaatacc aattaatact caagagcatt agcctgaatt
    12301 acgtaaacca gtcgctatgc cattgatagt tgtatggatc aatttttcat cattttcgtc
    12361 aagttgatta ttacgccaac gtttaatcaa ttcaatttga atatagttca acacattaaa
    12421 atacggcaag cgtgacttca agctatgctt aagggaagga ttatcctcca atagatcatc
    12481 atggtcttgg atagcaagaa taacattttt tgtcaattgc cactcatcca aaatttcata
    12541 aaaaacatct cgaacatcct gactttcagc tagttgtgca tactgaaagg caatattcat
    12601 gtttgattta gataatacca tatcaacatt tgataacaag gaatggaaaa aaggccacgt
    12661 ctgatacatg tgttggaggc gttcaaggtt accttgtgct cggtcaatat aacgtttaaa
    12721 ggctgaacct accccatacc aacctgggaa catgatgcga ttttgtgacc atgaaaagac
    12781 ccaaggaatg gcacgcaagc ctgtaatttc cgtaatcgtt ttacgggctg ctggtcttga
    12841 cccaatgttg agactggaaa cttccttaat cgggcttgcc tcaaagaaat aatcatagaa
    12901 atggggattg tcaaaaacca attttcgata aatggtattg ctatcagcaa caatgctatc
    12961 catgatttct cgaaaactat caatctcatt aggatctgtt atcatttgag tcaccatacg
    13021 attgatagaa gctgaaatca acatttctaa atggtaataa gccacatctt tattgccgta
    13081 tttgttttca ataatttccc cttgctctgt cagacgaatc cgatctttga tggaaccaaa
    13141 aggttgtgaa gtaatggcat cataagaagg gccacctcct cgaccaactg ttcctccacg
    13201 accatggaag aaggtgattt tcactccatg ttcctcacca atagccgtta attcattctg
    13261 agctttataa agagtccaac ccgatgccaa gtagccacca tctttattac tgtcagaata
    13321 gcctaacatg atttcttggt aattacgatt cgttgcaatc caagatttga caatgtcatg
    13381 gcttaaatag gctgccatga tgtcacgggc attatctaaa tcctcaatgg tttcaaagag
    13441 gggaacaatt tgcacacgag cccgctgctg gtcaaccaat cccacttctt taagcataat
    13501 ggccaactca aacatatcag agacactttc tgtatgtgaa ataatatgtt gctttataac
    13561 ctcttctcca agcttatctt tcaattctcg tgccgtagta tagatagcca gttctttttc
    13621 taataggtct gatttagcaa ctgccgctga ggataaagtc cttggctcct ccattaattg
    13681 ttgtaagaga acatcgcatt tttcagtttc tgaaagagag ctataatcgt caacaatatt
    13741 ggctcctttt aataactctg ccacacaaga ttcttggaca ctggagtctt gcctcatgtc
    13801 aatgcttgct aagaagaagc caaaaatatc aacagcttgc atgacttctc gcaaatcccc
    13861 ctcaatcaaa gcggagttgc cattatcaag tagcgactgc tcaatagcct ttagatcagc
    13921 tttaaaatca acaggagagt cataggctaa gatgggattt tctaaactag ctggtgaaga
    13981 ccaagcagat gtgcttagtc ccacactact actcatacga gctgctggct gattagttag
    14041 ttgaatttgt gtttgtttaa gacgcgattg aatataatga aaagctctgc gataaggttc
    14101 atttccacgg taaatcgatt ggtcctgaga caaactggct aaacgttcca cttccgagtt
    14161 gggttgtacc aaggtcgacg acagtgaaaa tgttcggtaa agggctgcta atttgtcaat
    14221 gtagtaattg aggataacct cactttgaac cgtagctgac aagcacaagg tttcagctgt
    14281 gacaaagggg ttcccatcac ggtccccacc aatccacatc cccatggtaa tgggtttggg
    14341 attgtctaac tctagaccgt gtttttgggc taagtttttg tactctgttg tcaacttggt
    14401 cactgcttga atgagagaac catcgtaata ctgcatgaca ttttttatct cgtttttcac
    14461 ctgaagcttt ttctcgcgaa taatatctgt ctgcatgatc atttcaatat aacgatatag
    14521 ctcctgacgc catttctcaa gattaatcac accagcctta gcatcacggt atttgcgcag
    14581 aagtttatga atgtgagttg tcaattccag aatcgtttta cgttgtactt gcgtcggatg
    14641 tgctgttaaa acaggaacca cattaacctg ctccaaaatg tctttaccat tatcttttcc
    14701 agcaagatcc ttgatggtta gggctaattt tcctaaataa tcagtatccg tattattttg
    14761 ataattgatt tcataggcta aatcgacatc ttctgaaata ttgattagca agggtaaaat
    14821 agaaaaatac ctggaaatca cttccatttc ttgatttgaa atattggcga caactttttc
    14881 taattctatg taatcttgtt tttcagataa caccataata gactcgataa ctgcaaacgc
    14941 atcatctcca atcatccgtc gtgtgatatt ttccaacatt tctttcaaaa gagcaacttc
    15001 ttcagcaatg atagcttgat tgttactact ctctaacttt ttaagtggca aatgaccatc
    15061 tccttctttg ctagtttctt ctatcatacc actttttacc aaaaataaaa gtgatcatgt
    15121 taaaaacgtt tgttttttaa caaaagtaag cgctttattt taaagaatgt ttggataggt
    15181 ttagtctcca tattccacta aaattctgtt atactagtct tgactatatt gttaaggaga
    15241 caactatgga attgaaaaaa cgttcagaat tccctgaaaa tgaactttgg gatctaacag
    15301 cgctctacaa agaccgccaa gattttttac tcgctattga aaaagcctta caagatattg
    15361 acctgtttaa gcgcaattat gaaggccgct taacgtcggt ggacgatttc actcaggcct
    15421 taatagaaat tgagcatatt tatattcaaa tgagtcatat tggcacttat gcctttatgc
    15481 ctcaaaccac tgacttttca gatgaaagtt ttgctcaaat tgctcaggct ggcgatgatt
    15541 tcatgactaa agctagcgtt gcccttagct tctttgacac cgccttagcc aatgccgacc
    15601 ttgacgtgtt agatacatta gaaaagaacc cttatttcag cgcagctatt cgcatggcaa
    15661 aaattcaaaa agaacacctc ttaagtcctg atgtggaaaa ggctttggct aacttacgcg
    15721 aggttatcaa tgcgccctat gatatttaca ccaaaatgcg ggctggtgac tttgacatgg
    15781 aggattttga ggtggatggt aaaacctata aaaatagctt tgtttcttac gaaaatttct
    15841 atcaaaatca cgaaaatgct gagattcgtg acaaagcctt ccgctctttt tcaaaaggtc
    15901 tccgtaaaca ccaaaataca gctgcagcgg cttacttagc caaggttaaa tctgaaaaat
    15961 tgttagcgga tatgaaaggc tacgcttctg tctttgatta cctcctagct gaacaggaag
    16021 tggatcgtag cttattcgac cgccaaattg acctcatcat gactgaattt ggaccagttg
    16081 ctcagaaatt cttaaaacat gtcgcccaag tcaatggtct tgagaaaatg acttttgctg
    16141 actggaagct agatattgac aatgatctca atccagaggt cagcattgat ggtgcctatg
    16201 acctcgtgat gaaatctcta gccccattag ggcaagaata cacgaaagaa atcgaacgtt
    16261 atcagacaga acgctgggtt gactttgctg ctaacgccaa taaggattct ggtggctatg
    16321 ccgctgatcc ctacaaggtt catccgtatg tcttaatgag ttggactgga agaatgtctg
    16381 atgtttatac ccttattcat gaaatcggtc actctggtca attcattttt tcagataatc
    16441 accaaagtta cttcaacacc catatgtcaa cttattacgt cgaagcgcca tcgaccttta
    16501 atgaattgat gcttagtgat tacttggaac atcaatttga tgaccctcgt caaaaacgtt
    16561 ttgcccttgc tcaccgttta acagacactt actttcataa cttcatcact cacctattgg
    16621 aagcggcttt ccaacgtaag gtttacaccc tgattgaaga aggcggtacc tttggagctg
    16681 accaattaaa tgcgatgatg aaagaggtct taacagactt ctggggagat gccgttgaca
    16741 tcgacgatga cgcagctctt acctggatgc gccaagcgca ttactacatg ggcttatata
    16801 gttataccta ttctgctggg cttgttattt caacagctgg ttaccttaac cttaaaaaca
    16861 acccagatgg ggcaaaagaa tggcttgact tcctaaaatc aggtggcagc cgtacgcctc
    16921 tcgatactgc aatgctcatt ggggcagaca ttgccaccga aaagcctctt cgagatacca
    16981 ttcaattctt gagtgatacc gttgaccaaa ttatcaatta caccgaggag atgtcacatg
    17041 cctaatctct atgattttac ggttaaggct caaaatggaa atgacctttc cttagctgct
    17101 tataaaaaaa aggttgtttt gattgttaac acagctacca agtgtggatt aaccccacaa
    17161 taccaggccc tgcaagcact atatgacaca taccatgaca agggatttga ggtcttagat
    17221 tttccttgca accaattttt aaatcaagct ccaggagatg ctgaggagat taatcacttt
    17281 tgcagtctga cctatcacac tactttccct cgctttgcta aaatcaaagt gaatggtaaa
    17341 gacgctgatc ctttgttcac ctggttaaaa gaagaaaaaa gtggtcccct tgggaaacgc
    17401 atcgaatgga atttcactaa atttctcatt gaccaaaatg gtcaagtcat taaacgctat
    17461 tcctcaaaga cagatcctaa actaattgaa gaagacctta aagcacttct tggttaatat
    17521 aaaatagact tggcgtgcca agtctatttt gtattaatag ttactattta aaaacttctt
    17581 gataaaattc aatgctatca ccatctttaa cggtaatttg atcggcaccc ttatctgcca
    17641 tctttccatt caccttaaaa agccagtatt tgttagcttt cgtgtcttgc tcaataccat
    17701 caattgctgt gataaagcca tctttttctt tgacctcata attatctttg agaatctcaa
    17761 gcaccgtatc tccttttcca aaagaaactt tttcatcaat ggtattggta tcttctttga
    17821 caactaagcg aacgtgatgg tctgcttttg gaacgcttgg agtctgtgtt tgttttgttc
    17881 cttgcgagca agcaacaagt agcatagcaa caagcgctag aaatcctgtt ttaaatcttt
    17941 tgttcaccga ttaacctcct aaataacatc atcacaactg gaaaaaagac gagagttgat
    18001 aaagcatgcg ctatattaaa gggcatccct gctaaaacat aactccacca aggcatacta
    18061 tacaaatacg caaaacaagt atctatcaac accccataaa ggaggccaca taaggcggcc
    18121 aagaaagcct gataaccaag ctgatatttg gggaattgct gagacaaagg gtacaaggca
    18181 aaacgccata ataaaagaac aagcacaaaa caagtaactt gccaaaagac ccaagggcca
    18241 aatcccagca aaaaggctga taaaaacaag cacaacatca taactaatac agcctcggct
    18301 aacccaaagt aaagaagata actgagtaaa aatgccgtca caggctgcac attaggcagg
    18361 ctgctaaaaa ccattctcaa cacaacacat agggctgaca tgatagcaat gcgactcata
    18421 aacttagctg acataaactt cttcctcgtt tttttctcta ttatgacaga tttacagagc
    18481 gtttaccagt ttgaagctca cttatttatc aaatgataat aacatgagaa ttaaataaca
    18541 aaacagtact tgctgactgt catgatatag tcaaagacta aactaatatt gtaaagaata
    18601 tagctaccaa ctataagaga tagtgacata aacttgacat agttagagag gatctcattt
    18661 gtaacttata aaagataata ccttaaaatg caagataata taagaaaaca gttaggatat
    18721 tttgattttt tttagtaatt agggtacaat aacagttaat gagaagaaaa ataaaaccta
    18781 ttgttgtgct agtcttcttt attttactgg caatggtatt gattatcgga aaaagacaag
    18841 ctaatcatgc gaaacaaaaa gaagttgaag acgctaagag tcatattcct attgcaacca
    18901 gtaatcctag taaagcaaaa actagcactt ctgaaaccga agactttatt ttaaatccta
    18961 ttgttgatgt ttccggctgg caactccctg aagaaattga ttatgacaca ttgtcacgtc
    19021 acatctctgg cgcaattgta cgtgtctatg gaggatcgca aattacagct cataacaacg
    19081 ctgcttttac aactggaatt gacaaatctt ttaaaacgca tatcaaagaa tttcaaaaac
    19141 gaaatgttcc tgtagctgtt tacagttacg cacttggtcg tagtactaaa gagatgaaag
    19201 aagaggcccg agctttctac aaaaatgctg ctccctataa tccaacttac tactggattg
    19261 atgtcgaaga agccactatg aaagatatga ataaaggcgt aacagccttc cgagaagagc
    19321 ttaaaaaact tggtgctgaa aatgttggtc tctatattgg aacttatttt atggcagaac
    19381 aagatatttc aacaaaaggt ttcgatgctg tatggattcc gacttatggt agtgattctg
    19441 gctattatga ggctgctcct aatacaacct tagattacga tttacaccaa tatacctcac
    19501 aaggttatct cagtggtttt aacaatgctt tagatttaaa tcaaattgcc gtaacaaaag
    19561 acactaaaaa aacgtttgag aagttatttg gcaactccaa caattaagag gttagcatca
    19621 tggctaatct ttttttatag tttacatctt accaagaaaa actctaggtc aataatttaa
    19681 aaccccttat ccttgacaag gagattatct catcatataa tgaaagcact ataatatttc
    19741 acaaaggagt gctttggctg agatcgcaat tgcgaaatcc tgaggacctg atcttgttag
    19801 tacaagcgta gggattgtga ccaataatca atgattggaa agcgatactc tttttgtgca
    19861 caaaaggagt tttttatgtc accaaacacc aatgtcaaat accttatcga agctgctatt
    19921 tttgctgcac tggcgatggc cctttcattt attccagatt ttgctggctg gtttagtcct
    19981 tcttatggtg ctattgcact ggttattttc tctttgcgcc gtgggttaaa gtacggcatg
    20041 ttggcgggtc tcatttgggg gctgcttcac tttgtacttg gaaaagttta ctatctcagc
    20101 atgtcccaag tctttattga atatatctta gcctttacct ctatgggatt agccggtagt
    20161 ttctctgata gtttgattaa aactttaaga agacaacaaa ccttttttgc cgtttttctt
    20221 gcaataatgg ctagtttact agccgttaca gtacgttact tgtggcactt ccttgcagga
    20281 atcattttct ggggcagtta cgcccctaaa ggcatgtccg ctgtctggta ttcttttagc
    20341 gttaatggta cagctggtgt cctaactttt ttaataacat gtttagcgtt aatgattgct
    20401 ttaccaattc atcctcaatt atttgatccc aaagactaat ttttagtcat ttgaatctta
    20461 cattaaaaaa atggtataat aatgttgtta taccattttt tatgtttaga taaaggtaag
    20521 gacttgatat ggctaaaaaa acaaaattca aaaaaacctt agtagaacaa atcctcgata
    20581 aggctaatat tgctcatcaa ggactcaagc ttaatgcttt agaaggcgac tttccagatg
    20641 atcttcagcc atcagatatt tacaagacac ttgctctgac tggtgatcaa acaggtcctc
    20701 taattggcat tattccatta acagaacact tatcagaaaa acaattggca aaagtatcag
    20761 gtaacaaaaa agtgtccatg gttcctcaaa aagacttgca aaagacaaca ggctatattc
    20821 acggtgccaa taatcctgtt gggattcgtc aaaaacattc atatcctatt tttattgacc
    20881 aaactgcact ggaaaaaggt caaataatcg tttcagctgg tgaagttggg cgttctataa
    20941 agatttctag ccaggctcta gctgatttcg ttggcgcaag ctttgctgat ttaaagaaga
    21001 gaaaatgaac actactgcca actttatctg gtttcttttt taagtttttc gccttgatta
    21061 agctagtctt tgtttagtat tattttgatc tcaactgctt taacattgat cctgttaatc
    21121 tttttctttt gtaaaggctt gatatcacga caaattcagg tcgatttgtc ttaaatctaa
    21181 tatgggcttt acactttaat cagaacctag ctattataca cttatactac gcttttttac
    21241 tttatctttt cactataggt attatttttg cttggtgatg cctaaacttt ttctggttac
    21301 ttttttattt ttagcgctcg catcagttat ctctaaaagg aggcttaaat gaaattatat
    21361 tttgtaagac atgggaaaac cctttggaat ttagaaggtc gctttcaagg cgctgggggc
    21421 gattcgccat tacttgaaga ggcaaaagac gaaattcact tacttggcaa agagttagct
    21481 aaggtggctt ttgatgccgt ttatactagc gaccttcaaa gagctatggc aacagcagct
    21541 attatcttag atgcttttga ccaacagcca aaactttacc acaccgacca gcttcgggag
    21601 tggagattag gtaaattgga aggggctaaa atagcaacaa tggcagctat atatccccaa
    21661 caaatgctgg cttttcggga gaatctagcc cagttcaaat cagatcagtt cgaggctgaa
    21721 tcaatttacc aaacgactca acgtgtctgc catcttatcc aatcgtttaa agataaacat
    21781 tatcaaaacg ttcttattgt aggacacggc gctaacttaa cagcaactat tcgctcatta
    21841 ttaggttttg aacctgctct tttactcgct aa
//
LOCUS       DAUVUY010000023        21805 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.023, whole genome shotgun sequence.
ACCESSION   DAUVUY010000023 DAUVUY010000000
VERSION     DAUVUY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21805
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.023"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            89..784
                     /locus_tag="VOX58_000772"
     CDS             89..784
                     /locus_tag="VOX58_000772"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HES5878019.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQILMTFLENLPV"
     gene            935..1276
                     /locus_tag="VOX58_000773"
     CDS             935..1276
                     /locus_tag="VOX58_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878020.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGRYRQGLTD
                     EKSKLQYSKDNAKLQLRNILNGYQSDLGRHYSSYYYYNLRTVMGLSKEQEIEKYYEEL
                     KNKLHDMYNHY"
     gene            complement(1375..2022)
                     /locus_tag="VOX58_000774"
     CDS             complement(1375..2022)
                     /locus_tag="VOX58_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HES5878021.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLSINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
     gene            2167..3099
                     /locus_tag="VOX58_000775"
     CDS             2167..3099
                     /locus_tag="VOX58_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HES5878022.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            3163..3888
                     /locus_tag="VOX58_000776"
     CDS             3163..3888
                     /locus_tag="VOX58_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HES5878023.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            3889..4743
                     /locus_tag="VOX58_000777"
     CDS             3889..4743
                     /locus_tag="VOX58_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HES5878024.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLVVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(4890..5696)
                     /locus_tag="VOX58_000778"
     CDS             complement(4890..5696)
                     /locus_tag="VOX58_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HES5878025.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPKQDITITSIDIVKDYRFKN"
     gene            5913..8318
                     /locus_tag="VOX58_000779"
     CDS             5913..8318
                     /locus_tag="VOX58_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HES5878026.1"
                     /translation="MVKRNQRRKSAPKKCLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQDIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMPKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVDVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FLMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKVGVRNIA
                     GYNAKVEDYNRQSEQKQMPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(8388..8741)
                     /locus_tag="VOX58_000780"
     CDS             complement(8388..8741)
                     /locus_tag="VOX58_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HES5878027.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTPLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            8985..9410
                     /gene="rplK"
                     /locus_tag="VOX58_000781"
     CDS             8985..9410
                     /gene="rplK"
                     /locus_tag="VOX58_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HES5878028.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            9516..10205
                     /gene="rplA"
                     /locus_tag="VOX58_000782"
     CDS             9516..10205
                     /gene="rplA"
                     /locus_tag="VOX58_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HES5878029.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            10527..11255
                     /locus_tag="VOX58_000783"
     CDS             10527..11255
                     /locus_tag="VOX58_000783"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HES5878030.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            11284..11841
                     /gene="frr"
                     /locus_tag="VOX58_000784"
     CDS             11284..11841
                     /gene="frr"
                     /locus_tag="VOX58_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HES5878031.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAIKEIDRMTAEKEKELLSV"
     gene            11950..12807
                     /locus_tag="VOX58_000785"
     CDS             11950..12807
                     /locus_tag="VOX58_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HES5878032.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            12880..13389
                     /gene="msrA"
                     /locus_tag="VOX58_000786"
     CDS             12880..13389
                     /gene="msrA"
                     /locus_tag="VOX58_000786"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HES5878033.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPEEISYKELVELYWVQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPRRYAQSSAIR
                     HQFLEENWS"
     gene            13386..13601
                     /locus_tag="VOX58_000787"
     CDS             13386..13601
                     /locus_tag="VOX58_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HES5878034.1"
                     /translation="MRKSFYSWLMTQRNPKSNAPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            13757..14938
                     /locus_tag="VOX58_000788"
     CDS             13757..14938
                     /locus_tag="VOX58_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HES5878035.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTQIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTIPMAPSATPSDVPTTPLASAKPDSFVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEASSAAPAQAPAEKEETSQMLTAPAAQKAVADTTSVATSNGLSYAPNHAY
                     NPMNAGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPVSSTLGDQV
                     AQYAIDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFN
                     A"
     gene            15253..17025
                     /locus_tag="VOX58_000789"
     CDS             15253..17025
                     /locus_tag="VOX58_000789"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HES5878036.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELVHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWIYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKDNETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            17184..18236
                     /locus_tag="VOX58_000790"
     CDS             17184..18236
                     /locus_tag="VOX58_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HES5878037.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            18282..18857
                     /locus_tag="VOX58_000791"
     CDS             18282..18857
                     /locus_tag="VOX58_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HES5878038.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARLIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            19016..19513
                     /gene="ybeY"
                     /locus_tag="VOX58_000792"
     CDS             19016..19513
                     /gene="ybeY"
                     /locus_tag="VOX58_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HES5878039.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDFAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            19494..19901
                     /locus_tag="VOX58_000793"
     CDS             19494..19901
                     /locus_tag="VOX58_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HES5878040.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            20021..20917
                     /gene="era"
                     /locus_tag="VOX58_000794"
     CDS             20021..20917
                     /gene="era"
                     /locus_tag="VOX58_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HES5878041.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            20937..21413
                     /locus_tag="VOX58_000795"
     CDS             20937..21413
                     /locus_tag="VOX58_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HES5878042.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
BASE COUNT         6723 a         3848 c         4731 g         6503 t
ORIGIN      
        1 ttaaatgtga ttttgatagc tgttatgctg ttaattgcca tcttggtata tgctatcttt
       61 tatctttaaa aaaacgtaag gaaatgatat gaaaatagga attattgctg ctatggagga
      121 ggagttgtcc ctgctcttag caaatctttt ggatgctcaa gaacatcagg tgctttcaaa
      181 aacttactat acgggtcgtt ttggcaagca tgagctcatt ttggtacagt caggtgttgg
      241 aaaagtgatg tctgctatga cagttgcgat cttggttgag catttcaaag cccaagctat
      301 tatcaataca ggatcagcag gggcagtcgc ttcacattta gcgattggag atgtggtggt
      361 tgcagatcgg ttagtgtatc atgatgtgga tgctactgca tttggttatg cttatggtca
      421 aatggctggt caaccattat actatgattg tgatccacag tttgtggcta tcttcaaaca
      481 agtcctaaaa cacgaaaaga caaatggcca agttggtttg attgcaacag gagacagttt
      541 tgttgctggt caggacaaga ttgaccagat caaaacagct tttagcgacg tgttagcagt
      601 agaaatggaa ggcgcagcta ttgctcaagc agcacatact gcagggaaac ccttcatcgt
      661 tgttcgtgcg atgagtgata ccgcagcgca tgatgccaat attacttttg atcaatttat
      721 tatagaagct ggcaagcgtt cggcccagat attaatgaca tttttagaaa acttacctgt
      781 ttaactcacc ttatcgtgag acaattagct ggataatctc tattttagga gtctggtcac
      841 ttcttgtttt gaaacctaaa aataagaatt gcaatcactt gaccaaacgc tataataggt
      901 taagtaacga tagattacta aaaggagtct aaaaatgaca ctaaaaaaac actattatct
      961 tctcagtctg ctagctcttg taacggttgg tgctgccttt aacacaagcc agagtgtcag
     1021 tgcacaagtt tatagcaatg gaagatatcg tcaaggtttg actgatgaaa aatcaaagct
     1081 gcaatatagt aaagacaacg ccaaacttca attgagaaat atccttaacg gctaccaaag
     1141 tgacctaggg agacactact ctagctatta ttactacaac ctaagaaccg ttatgggact
     1201 atcaaaagag caagagattg aaaaatatta tgaagagctt aagaacaagt tacatgatat
     1261 gtacaatcat tattaagtta aaggcttaaa aatggagact agcccctcaa aaaaagaggc
     1321 cacactcatt taaatttaaa tgagtgtggc ctctttttga ggagacgcaa ggggttaaag
     1381 ggctgtgaca tagagttgct tagcaatatt ttcagggatg acaagttcct tgtcgccata
     1441 acaaatcgta taggtttttg caaaggtatc aatttgagtt aaggttagct cggtattgat
     1501 actcaggtga tgtgcttcta gatactggat taaatcaaag tggtcgtgga ttctgcttag
     1561 gcgaaaccgt cccaactccg tgatggtgtt gagagttgta gtgttcattt cgacaagagg
     1621 ctgaccatag cgaggaatag tccctccgtg tgggcagaaa tctggaaaat caaggatctt
     1681 gtctaaacgg tcgataaagg tgtctgagac agtgtgctct aagacttcag cttcttggtg
     1741 cacttcttgg gtattatagc ccagctggtg aatcaaaaat acctcaatca agcggtgttt
     1801 gcggtagagg ttagcaacaa gagcatagcc tttgtctttt agcaaatagc cttttgcttt
     1861 atctttgaca atccagcctt gtgaaatcat ttttttaatc atctctgaaa cggcaggtgc
     1921 cgaaacgtgc atcttttctg caaccatttt atttgtaatc ttgggttctt gttctccaat
     1981 ttcatagata catttcaagt aatcttcttt attaggcgtc atatgtttgg cctttctctt
     2041 atttataatg attattatat cagaattttt aggaaaaagc ttgacaaaaa gcctaaaaga
     2101 ggatatcctt atagcataat taactaaact taattaaata aataatatat cctaaggaga
     2161 gaaatgatgg gaaagagaat gagcctcatt ttgggtgctt ttttatctgt ttttctttta
     2221 gtagcttgtt cgtcaactgg cactaaaact gctaagagtg ataaattaaa agtcgtggca
     2281 accaattcaa ttattgccga catgacaaaa gctattgctg gtgataaaat cgatctgcac
     2341 agcattgtgc caatcggtca agaccctcat gagtacgaac cattaccaga agatgttgaa
     2401 aaaacaagta atgctgatgt gattttctat aatggtatca atctagaaga tggtgggcaa
     2461 gcttggttca ccaaactagt gaaaaatgct caaaaaacga aaaacaaaga ttactttgcc
     2521 gtgtctgatg gcattgatgt gatttacttg gaaggtgcaa gcgaaaaagg aaaagaagat
     2581 ccacatgctt ggttaaatct cgaaaacgga atcatttatt caaaaaacat tgccaaacaa
     2641 ttgattgcaa aggatcctaa aaacaaagaa acttatgaaa agaacctaaa agcttatgtg
     2701 gctaaattgg aaaaactaga caaagaagcc aaatcaaaat ttgatgctat tgcagaaaat
     2761 aaaaaattga ttgtgactag tgaaggctgc ttcaagtact tttcaaaagc ttacggtgtc
     2821 ccatctgctt atatctggga aattaacacc gaagaagaag gaacaccaga tcaaatttca
     2881 tcattgattg aaaaactaaa agtcatcaag ccatctgcgc tttttgtaga gtcaagtgtc
     2941 gatagacgcc ctatggaaac cgtttctaaa gatagtggta ttcctattta ttctgagatc
     3001 tttacagatt caattgctaa aaaaggtaaa cctggcgata gttattatgc tatgatgaaa
     3061 tggaaccttg acaaaatttc tgaaggtcta gcaaaataag ataacaataa agcacagcga
     3121 ttttagtgtt gtgctttgtt ttatttatgt aaaggagaca aaatgattac aactaacaat
     3181 ctttgtgtga cctacgatgg caacagtaat gctttagaag cgattaatgt gacaattgaa
     3241 ggtccttcta tcgttggtat tattggtcct aatggagctg gaaaatcaac ctttatgaaa
     3301 gctattttaa atcttattga ttatcaaggt cacgtaacgg ttgatggcaa agatggtcgt
     3361 aagcttgggc atacagtagc ttacgtagaa caacgaagca tgattgacta taattttcct
     3421 attactgtta aagaatgtgt ggcgctaggg acttatagta aattaggcct ttttcgccga
     3481 gtaggaaaaa aacaatttga gcaggttgat aaggttctca aacaagttgg tttagaagac
     3541 tttggccatc gaccgataaa atccttatct ggtggtcagt ttcagcggat gttggttgcg
     3601 cgttgcttga ttcaagaatc agactatatt ttcttagatg aaccgtttgt tggcattgac
     3661 tcggtcagcg aaaaaatcat tgtagatctt ttgaaagagc tcaaaatggc tggcaaaacg
     3721 attttaattg tacatcatga tttgagcaaa gtagagcatt attttgacaa actcatgatt
     3781 ttaaataaac atttggtggc ttatggcaac gtttgtgagg tatttactgt tgataccttg
     3841 tcaaaagcct atggcaatca tttgatttta ggaaaggaga tggtgtaaat gtctatgaaa
     3901 ttttttgagg gcttgatgtc ataccacttt ttacaaaatg cactgataac ggctgtagtc
     3961 attggtatcg tctcaggtgc tgtagggtgt tttattatcc ttaggtcaat gtctcttatg
     4021 ggtgatgcca tctcacacgc tgttttacca ggggttgctt tgtcatttat tttaggagtc
     4081 aattttttta ttggagcgat tatttttggg ttattagctt ctgttattat tacttatatc
     4141 aaggaaaact ctgtcattaa aggagatacg gctattggta tcacctttag ctcttttttg
     4201 gcactgggag ttatcttgat aggggtagcc aatagttcga cggacttatt tcatattttg
     4261 tttgggaata ttttagctgt gcaagatagc gataagtgga ttactattgg tgtttcgatt
     4321 tttgttttag tggttattag tctctttttc aaagaactat tattaacatc atttgatcct
     4381 atcttagcca aatcaatggg tgtgaaggtc aatgcgtatc attatttgtt gatggtccta
     4441 ttaactttag tggctgttac ggcgatgcaa agcgtgggta ctatcttgat tgttgcctta
     4501 ctgattacgc cagcagcgac agcttattta tatgctaata gcttaaaagt aatgttagtg
     4561 atgtcatctt tactaggtgc tttagcatcg gttttagggc tttatttggg ctataccttt
     4621 aatgttgccg cagggtcaag tatcgtactg acttctgcga tgatgttttt gatcagtttc
     4681 tttgtttcac ccaagcaagg ctacctcaaa agatggatgc aaaaaaaaga aaaaacacct
     4741 taaacttttc gctttaaaca ccttagtggt ttgcaaatag tggcaaataa catcaaaggg
     4801 tgttttttat atgtcatcaa aagaccataa aaaaccttat caaaccaagg tatttagcct
     4861 attatctgat aaggtttttt aagcatgatt tagtttttaa agcgataatc tttgacaatg
     4921 tcaattgagg taatcgtaat gtcttgtttt ggtttatcat tttggttgat agaagtcgcg
     4981 gcaattttat cgacaacatc cataccatca atgacttgac caaatacagt ataaccgcca
     5041 tctaagcttg gattgccgcc atgctcatag gcagagatga taggttttgg gtagttggta
     5101 cttgataatc ccttgctttg atttttcttg ttttggttga tataaaattg gctaccgtta
     5161 gtattagcgc cagcatttgc catggcaaga gcaccgcgaa tatgatataa aaatggagag
     5221 atttcgttga caaagccatt gccagcatct tttttaggat ccttgccctt ccaaatcgat
     5281 tcaccacctg tgccatctcc tttggggtca cctgattgaa tcataaagtc gttgatcaca
     5341 cgatggaagg taaggttatc atagtagcct tttttagcat gggtgaggaa gttctcaaca
     5401 gctaagggag catattttgg gaataactta agggtgatgt ccccttgact tgtcctcatt
     5461 acgaccttag cttcttcttt gccgacttct ttggttagtt gtggaaattg gctagcatca
     5521 gctttgagag ctttttgaat gctttcttca taggctttag aagctgcttc gctttcttct
     5581 ttggcagttt tttcatcaat gtatttatcg ccttttatag cgcggtcaac agattcacaa
     5641 gcactcaaac taagtaaact aatggctact aaactgagag ataataattt tttcatgtct
     5701 actcctaaga atgattttct ttattttatc ataaaatgca acagaaaata gtagtaaaga
     5761 gttaaaaacg cctatttttg tgttttatga tctaaccttg gttaaaaggc tgctacgact
     5821 gttattatag cacaagatgc tatagaaaaa cttaaaacac cttggttttt tagagctatt
     5881 tcctaatagt taaaaaatgg tataatagtg ttatggttaa aagaaatcaa agaagaaaaa
     5941 gtgcgcctaa gaagtgtctg acaaaagcag aagtggaaaa gcaacgtgcc attaagagga
     6001 tgattttatc tgttttaatg gctttgttgc ttatttttgc gatgcttcgc ttgggcgtct
     6061 ttggcgtgac aacttataat atgattcgtt ttttggtggg tagtttagcc tatccgttta
     6121 tgtttgcctg gttaatttac ttattttgtt ttaaatggtt acggcaaaaa gatggaatga
     6181 ttgcgggagt tgttattgcc tttttgggat tgctagttga gtggcacgcc ttcttatttg
     6241 cgatgcctag gatgcttgat caagatattt ttctaggcac tgctcggcta attacaagag
     6301 atttactagc cctgcgcgtg actgaatttg ttggtggagg aatgcttggt gcattacttt
     6361 ataaacccat agccttttta ttttcgaata tcggctctta ctttattggt tttttgttta
     6421 tcttattagg attatttcta atgacgcctt gggatatcta tgatgtgagt cactttgtga
     6481 aagaggctgt tgataaacta gcagtggctt atcaggaaaa taaagaaaag cggttcatca
     6541 aacgagaaga gcaccgctta caagctgaaa aagaggcttt agagaaacaa gcacaggagg
     6601 aagaaaaacg tctagcagaa ttgacggttg atcctgagac gggagagatt gttgaggata
     6661 gtcagtctca agtcagctat gatttggctg aggatatgcc aaaagagcca gagattcttg
     6721 cttatgatag tcacctcaaa gacgatgagg caagcttatt tgaccaagaa gacttggctt
     6781 acgctcatga ggaaattggg gcttatgact ctttatctgc tttagcatct tctgaagatg
     6841 agatggatat ggatgaacct gttgatgttg atttcacgcc aaaaacccat cttctttata
     6901 aacttccaac gatagattta tttgcaccag ataagcccaa aaaccagtcc aaagagaaaa
     6961 acttagtccg caaaaatatc aaggtgcttg aagatacctt ccaaagtttt gggattgatg
     7021 tcaaggtaga gcgagcagaa atcgggcctt ccgtcaccaa atatgaaatc aagccagctg
     7081 taggggtccg agttaatcgt atttcaaatc ttgctgatga tttggcactt gctctggctg
     7141 ctaaagatgt tcgtatagaa gcaccaattc ctggaaaatc cttaataggt attgaagttc
     7201 ctaactcaga aattgcaacg gtttctttcc gcgaactttg ggaacaatct gatgccaatc
     7261 ctgaaaacct tttagaagta ccactaggta aagctgttaa tggtaacgct cgcagtttta
     7321 acttagctag aatgccgcat cttttggtag ctggttcaac tggttcaggt aaatcggttg
     7381 ctgttaatgg cattatttca agtattttga tgaaggcacg tccagaccaa gtgaaatttt
     7441 tgatggtcga ccctaagatg gttgagttgt ctgtttataa tgatattccc catttgctga
     7501 ttccagtagt gaccaatcct cgtaaagcca gcaaggcgtt gcaaaaagtg gttgatgaga
     7561 tggaaaatcg ctatgaactc tttagtaaag tcggggttcg aaatattgct ggttacaatg
     7621 caaaagttga ggattacaat aggcaatctg agcaaaaaca aatgccttta ccattgattg
     7681 tggttattgt ggatgagtta gctgatttga tgatggtggc tagtaaagaa gtggaagacg
     7741 ctattatccg tctggggcaa aaggcgcgtg cggcaggtat tcacatgatt cttgcaaccc
     7801 aaagaccatc tgtggatgtt atttctggac tgattaaggc caatgtgcca tcccgtattg
     7861 catttgccgt atcttcagga acagatagtc gaacgattct ggatgaaaat ggcgctgaaa
     7921 agctcttagg acgtggagat atgctcttta agccgattga tgaaaatcac cctgttcgtc
     7981 tgcaagggtc tttcatctca gatgatgatg tggagcgtat tgtcaacttt atcaaagacc
     8041 aagctgaagc ggattatgat gatgcctttg atcctggtga agttagcgac aatgatcctg
     8101 gtttttctgg aaatggcgga gctgctgaag gtgatcctct ttttgaagaa gcaaaagcct
     8161 tggtcttaga aacacaaaaa gctagtgcct caatgattca aaggcgtttg tcggttggat
     8221 ttaatcgtgc cacgcgcttg atggatgaat tggaagaagc aggtgttatc ggtccagcag
     8281 aaggaaccaa acctcgtaag gtattgcaga caaactaaaa gaacctgacg tctcatgatg
     8341 agatgtcagg ttcttttttt gtgatgtcaa gccagttgat gagatgccta tgctaatgta
     8401 aaaataaaga taactagaat caggtagaaa ataacggtga ctaaaaagcc aactcgccaa
     8461 aatagcttca taaagcgatg gtaagaaaac gactgagttc ggatgactag cagtagtgaa
     8521 atgacgatgg ctagcagcga catgataata aggtaataag gcagtaaatt atgggtgaaa
     8581 aattttcctg agacaagcac aatttctatc gcaaaaagta aaagtgcgac atctggaaat
     8641 ttaaatccct ttttattaag tttaaataaa ggggttacaa tcatggcaaa aagaggcgtt
     8701 actatcaaga aagctaaggc agttaattta tagatcatca taggtctatt ttatactcaa
     8761 atcccttact tgtaaactga tacttacctg ttgtgataga aaatgatctt tgagagggaa
     8821 agcgcctact tcaatttaaa cattacaaaa ggcatcagct attttaacaa aaagacttga
     8881 tttattttgg caataagcta taatggtagg gtatgcaata ttgcatatct gtgggaggta
     8941 aaaatctcaa ttaccgccaa aaccacaaca ggaggatttt taaaatggct aaaaaagtcg
     9001 aaaaacttgt aaaacttcaa atccctgctg gtaaagctac accagctcca ccagttggac
     9061 cagctcttgg tcaagcaggt atcaacatca tgggcttcac taaagaattt aacgctcgta
     9121 cagctgatca agctggtatg atcatcccag ttgttatctc agtttatgaa gacaaatcat
     9181 ttgatttcat cactaaaaca ccaccagctg ctgttctttt gaaaaaagct gcaggtgttg
     9241 aaaaaggatc aggtacacct aacactacta aggttgcgac agttactcgt gcacaagtac
     9301 aagaaattgc tgaaactaag atgccagatt tgaacgctgc aaacattgaa gctgcaatgc
     9361 gtatgatcga aggtactgct cgttctatgg gattcactgt tactgactag tcagtcaaga
     9421 acccctatta cccgcacgac ttcatcataa gagatgagtg acgtgggaga ttttaaatca
     9481 aatcgatatg accacattac aaggagaatt ttaaaatggc taaaaaaagc aaacaaatgc
     9541 gtgctgcact tgaaaaagta gatagcacaa aagcgtacag tgtagaagaa gctgtagcat
     9601 tagtaaaaga aacgaacttc gcaaaatttg atgcgtctgt tgaagttgct tacaacttga
     9661 acattgacgt tcgtaaagca gaccaacaaa tccgtggcgc aatggtattg ccaaacggaa
     9721 ctggtaaaac acaacgtgtt cttgtttttg cacgtggtgc aaaagctgaa gaagcaaaag
     9781 cagctggtgc agacttcgta ggtgaagacg accttgttgc aaaaatcaat ggtggatggc
     9841 ttgactttga cgttgttatc gcaacgccag acatgatggc tatcgtaggt cgtcttggac
     9901 gtgtccttgg acctcgtaac ttgatgccaa accctaaaac tggtacagta acgatggatg
     9961 ttgctaaagc cgttgaagag tctaaaggtg gtaaaatcac ttaccgtgct gacaaagcag
    10021 gtaacgtaca agctcttatt ggtaaagttt catttgatgc tgacaaattg gttgaaaact
    10081 tcaaagcctt ccacgatgta atggctaaag ctaaacctgc aacagctaaa ggaacttaca
    10141 tggcaaacgt ctcaatcaca tcaacacaag gtgttggtat caaggttgat cctaactcac
    10201 tttaagaaaa aaattagggc atcgcctaat catagcaaaa agacaaattt ctgaagacaa
    10261 atcttcacta gaggtttgtc ttttttatgg tttaaaatgg aatgcaaaca aaaaagtgta
    10321 ggcgataata ccatgaaagg agttgattct cttaatgaga tgtggtgtta acacatcatg
    10381 agctcacaaa aaatgataaa ggaagtatcg gtggtgttaa aaaagggatt ttaattgaca
    10441 tttattagcc tctaaaatgt cctttcccta ttcataattg agggtttgtg gtaaaatagt
    10501 aaggacaaac taaggagaac atagtagtgg aacctaaata tcaacgtata ttaattaagt
    10561 tatctggtga agcattagca ggtgaaaaag gagttggcat tgacattcca actgttcagg
    10621 ctattgcaaa agaaattgcc gaagttcatg tgtcgggagt acagatcgct cttgttattg
    10681 gtggaggcaa tctctggcgt ggagagcctg cagcagacgc tggaatggac cgtgttcagg
    10741 ctgattacac tggcatgctt ggaacggtga tgaatgccct agtcatggct gatagtcttc
    10801 aacattatgg tgttgatacc cgtgtgcaaa cagctattcc tatgcaaaat gtagcagaac
    10861 cttatattcg cggacgtgcc ttgcgccatc tagaaaaaaa tcggattgtt gtttttggag
    10921 ctggtattgg ctcgccttat ttttcaacag acaccaccgc ggcactgcgt gctgctgaga
    10981 ttgaagcaga tgctatttta atggcaaaaa atggtgtgga tggggtctat aatgctgatc
    11041 ctaaaaaaga tgctaatgcg gttaaatttg atgaattaac acatggtgaa gtgatcaaac
    11101 gtggtctcaa aatcatggat gcaacggcat cgaccttatc gatggacaac gatattgatc
    11161 tggtggtttt caatatgaat gaagctggca atattcaacg tgttgtcttt ggagaacata
    11221 ttggaactac tgtatcaaat aaagtttgtg actaaaagtt agaaaagaaa gagagtaatt
    11281 atcatggcaa atgcaattat tgaaactgca aaagaacgtt ttgcacaatc ccatcagtct
    11341 ttatcacgtg aatatgccag cattcgtgcg ggtcgtgcca acgctagtct tttagaccgt
    11401 atccaagttg attattacgg ggcaccgacg ccattaaatc aattggcttc aatcactgtg
    11461 ccagaagcgc gtgtcttatt gatctcgcct tttgataaat cttctatcaa agatatcgag
    11521 cgcgctctta acgcatcaga tttaggcatt acacctgcta acgatggctc tgtcattcgt
    11581 ttggttattc cagctttgac cgaagaaaca cgtaaagaat tagccaaaga agtgaaaaaa
    11641 gttggtgaaa atgctaaaat tgcaatccgt aatatccgcc gtgatgctat ggatgatgct
    11701 aaaaagcaag aaaaagcaaa agaaatcact gaagatgagt taaaaacact tgagaaagat
    11761 atccaaaaag taaccgatga tgctatcaaa gaaattgacc gcatgacggc tgaaaaagaa
    11821 aaagaattgc tctcagtgta acaggatttc tacgaaaaat agacgactat agacacaaaa
    11881 agataggtga ccatttcaga agcgtttggt tttgatttgt ccctattttt ttaaagaaag
    11941 aaaaaagata tgaatgattt actagcaaca gtcattactg ggcttattaa agaagaaaac
    12001 gctaatgact attttatcca aaaagagggg tttaccttta ccttgtctaa agcagaagga
    12061 gaacgccaga ttggtgatat ggtcacaggc tttgcctata cagacataaa gcaaaaggca
    12121 cgtctcacaa ccaaagagat ccgttcaaca cggacaagtt atggctgggg ggaagtgaca
    12181 gaggttcgtc gtgacctagg cgtttttgtc gatacaggaa ttccaaataa agaaattgtg
    12241 gtttctttgg acgtgttgcc tgaaatgaaa gaactatggc ctaaaaaagg ggataaactt
    12301 tacattcgtt tggatgtgga taaaaaagac cgtatttggg gacttccagc agagcctgag
    12361 gtctttcaaa agatggctag ccctgcatac aataatatgc aaaatcaaca ttggccagca
    12421 attgtttacc gcctaaaatt aacaggaacg tttgtttacc tgcctgaaaa caacatgtta
    12481 ggctttattc acccaagtga acgctacgca gaaccgcgct taggtcaggt tttagatgcc
    12541 cgtgtgattg ggtttagaga ggtcgatcgt accctaaact tgtcgttgaa accacgttct
    12601 tttgagatgt tggagaatga cgctcagatg attgtcactt atcttgaagc caatggtggc
    12661 ttcatgacct taaatgacaa atcaagtcca gaagagatta aagcaacctt tggtatttca
    12721 aaaggccagt ttaaaaaagc cttgggtgga ttgatgaaag ctaaacgcat caaacaagac
    12781 gccacaggaa ctgaattaat aggataaaaa tgctttgcca ctggcaaagt tttttgttac
    12841 aatgaagacc attaagtgaa gaaaaagggg atatagagaa tggaaagagc catttttgca
    12901 ggaggttgtt tctggtgtat ggtgcaacct tttgaggagc aagcaggtat tttgtcagtc
    12961 agaagtggct acacaggtgg gcatctccca aatcctagct atgaacaagt ttgtgccaaa
    13021 acaacaggac atacagaagc tgttgaaatt atttttgacc ctgaagagat cagctataag
    13081 gaattagttg aattgtattg ggtgcagacg gatccaacag acgcctttgg gcaatttgaa
    13141 gatcgtgggg acaactaccg tcctgtcatt tactacacaa cagaaagaca aaaagaaatt
    13201 gcagagcaat caaaagcaaa cttgcaagct tcaggacgtt ttgaccaacc cattgtcacc
    13261 actattgaac cagcagagcc attttatctc gcagaggatt atcaccaagg attttataaa
    13321 aagaatccca ggcggtatgc acaaagcagt gctattcgtc atcagttttt agaggagaat
    13381 tggtcatgag aaaatcattt tacagctggc tgatgacaca acgtaaccct aaatcaaatg
    13441 cacctgtggc gattttagca gatttggtgt ttgatgacac cacttttcca aagcatacca
    13501 atgattttga actcattagt cggtatctag aagatcaagc tagtttttcc tttaatttag
    13561 gtcaatttga tgaaatctgg gaagattatc tagcacatta atacgtggat aagaaagagt
    13621 catatcgact cttttgaagg gttatcacgt tattattaca aaatgacatc actttctttc
    13681 aaaaaaatga caagctactt tttttcttaa aaatatgcta tgatatagct tagaaaaaaa
    13741 taaaaaggaa gcagagatga ttattactaa aaagagttta tttgtgacaa gtgtcgcttt
    13801 gtcgttagca cctttggcga cagcgcaggc acaagagtgg acaccacgat cggttacaca
    13861 aatcaagtct gaactcgtcc tagttgataa tgtttttact tatacagtaa aatacggtga
    13921 cactttaagc acaattgctg aagcaatggg gattgatgtg catgtcttag gagatattaa
    13981 tcatattgct aatattgact taatttttcc agacacgatc ctaacagcaa actacaacca
    14041 acacggtcag gcaacgactt tgacggttca agcgcctgct tctagtccag ctagcgttag
    14101 tcatgtacct agcagtgagc cattacccca agcatctgcc acctctcaat cgactattcc
    14161 tatggcacca tctgcgacac catctgatgt cccaacgaca ccattagcat ctgcaaagcc
    14221 agatagtttt gtgacagcgt catctgagct cacatcatca acgaatgatg tttcgactga
    14281 gttgtctagc gaatcacaaa agcagccaga agtaccacaa gaagcagttc caactcctaa
    14341 agcagctgaa acgactgaag tcgaacctaa gacagacatc tcagaagatt caacttcagc
    14401 taataggcct gtacctaacg agagtgcttc agaagaagct tcttctgcgg ccccagcaca
    14461 agctccagca gaaaaagaag aaacctctca gatgttaact gcgccagcag cacaaaaagc
    14521 tgtagctgac accacaagtg ttgcaacctc aaacggcctt tcttacgctc caaaccatgc
    14581 ctacaatcca atgaatgcag ggcttcaacc acaaacagca gccttcaaag aagaagtggc
    14641 ttctgccttt ggtattacgt catttagtgg ttaccgtcca ggagatccag gagatcatgg
    14701 taaaggatta gccattgact ttatggtacc ggttagctct acgcttggtg atcaagttgc
    14761 tcaatatgcc attgaccata tggcagagcg tggtatttca tacgttattt ggaaacagcg
    14821 attctatgcg ccatttgcaa gtatttacgg accagcctac acatggaacc ccatgccaga
    14881 tcgcggcagt attacagaaa accattatga tcatgttcat gtctccttta atgcttaatt
    14941 catgctatca aaaaaactca cgtttaggcg tgagtttttt gacaattctg acagagtaag
    15001 ccctataaga ggctgagata cgcttttaat acctgttgtt tttctcgccc tttagacagt
    15061 tttaacgtta tgtctaaaag agatggtttt tattagcgca aatatgggtt taggggtaaa
    15121 atatagataa ttaacctgat atgatcatga taaaggtaga agaataccta ttatttcgtg
    15181 acaactgagc aatctgccct tacctttgga gtgatgatat cactttgctt gactattcaa
    15241 ggaggtcttg aaatgtatta tactagtggt aattacgaag cttttgcgac acctcgaaaa
    15301 cctgaagggg tagatcagaa atcggcttat attgttggta ctggtttagc tggtttagca
    15361 gcagctgttt tccttattcg cgatgggcat atggctgggg aacgcattca tctgtttgag
    15421 gaattgcctt tagcaggtgg ttctttagat ggtattgaaa agcctcatct tggttttgtg
    15481 acccgtggtg gtcgtgagat ggaaaatcat tttgagtgta tgtgggacat gtatcggtct
    15541 attccctcac tggaaattcc tggtgcgtct tatttggatg aattttattg gttggataag
    15601 gatgatccta actcatccaa ctgtcgtttg attcacaaga gaggaaatcg tgtggatgat
    15661 gacggccagt atacgctcgg taaacagtca aaagaattag tccatttaat catgaagaca
    15721 gaagaatctc taggagatca aaccattgaa gagttcttct cagaagattt ctttaagagt
    15781 aatttttgga tctattgggc aaccatgttt gcttttgaaa aatggcattc tgctgtagaa
    15841 atgcggcgct atgcgatgag gtttatccac catattgatg gtttgccaga tttcacctct
    15901 ctcaagttca acaaatataa ccaatatgat tctatggtca aaccgattat tgcttaccta
    15961 gaatcacacg acgttgacat ccaatttgac acaaaagtca ctgatattca ggtggaacaa
    16021 acagctggta aaaaggtagc aaaaaccatc catatgacgg tgtctgggga ggctaaggcg
    16081 attgagctaa cacctgatga tttggttttt gtgaccaatg gttctattac tgaaagtagc
    16141 acatacggta gtcatcacga agtggctaag ccaaccaaag cgttaggtgg ttcttggaat
    16201 ttatgggaaa atctagctgc tcaatcagat gattttggtc atcctaaagt gttttaccag
    16261 gacttgcctg ctgaaagctg gtttgtgtct gccacagcaa ccataaaaca cccagctatc
    16321 gagccttata ttgaacgttt gacccaccgt gatttgcacg atggcaaagt gaacactggc
    16381 ggcatcatca ctattacaga ttctaactgg atgatgagct ttgccattca ccgtcaacct
    16441 cattttaaag aacaaaaaga taatgaaacc actgtctgga tttacggtct ttattccaat
    16501 agtgagggca attacgtcca caagaaaatt gaggagtgta caggtcaaga aatcacagaa
    16561 gaatggttgt accaccttgg ggtacctgtt gataaaatca aggacttagc gagtcaggac
    16621 tatatcaata cagttcctgt ttacatgcct tatattacga gttactttat gccacgtgtc
    16681 aaaggagacc gtccgaaagt tatcccagat ggttcagtca acttggcctt tattggtaac
    16741 tttgcggaat ctccatctcg agatacggtc tttacgactg agtattctat tcgtactgcc
    16801 atggaagcag tgtatagctt cttgaatgtg gaacgaggca ttccagaagt ctttaattca
    16861 gcctatgata ttcgtgaatt gctcaaagcc ttttattacc ttaatgataa aaaggcaatc
    16921 aaggatatgg atttgccaat tcctgcactg attgagaaaa tcggacataa aaaaatcaag
    16981 gataccttta tcgaagaatt gctcaaagat gctaatctta tgtaacatgc cttagagtag
    17041 ttaaagctga taagtttagg cttatcagct tttttgatga tttatagaaa agtgataaag
    17101 gaaaagtctg acgtgaacgt cacataagaa tttaaaattt gttataatgt aagcaataaa
    17161 gatgaaagaa agagggactc tatttgcaag agtattctat tgacattacc ttaacccatc
    17221 ctgatgatgt tttagctctt tttggtagca acgagcgtca tttgaagcta atagaagctc
    17281 atttaggagt tattgttcac gcaagaaccg agcgagtcca agtgattggt gatgatgaag
    17341 aagctgtgga attggctcga ttgaccatca aagccctact ggttctagtg ggtcgtggca
    17401 tggtggtcaa cacatcagat gtggtgacag ctttatctat ggcagaatct catcagattg
    17461 accaatttat ggcgctttat gaagaagaaa tcattaaaga taactatggt aaggcaatac
    17521 gagtcaaaac attaggtcaa aaaacctatg ttgatagtgt caaacgtcat gatgtggttt
    17581 ttggtgtagg acctgcaggg acagggaaaa ccttcttagc tgttacgctt gcagtcactg
    17641 ctctgaaaag aggacaggta aagcgtatta ttttgacgcg tcctgcggta gaagctggtg
    17701 aaagcctagg ttttttacca ggcgatttga aagaaaaggt ggatccttac ctcagacctg
    17761 tttacgatgc cctttatcac attttaggca aggaacaaac cacacgcctg atggaacgag
    17821 atgtgattga gattgctccg ttagcttata tgcggggtcg tactctggat gacgcttttg
    17881 ttatcttaga tgaggctcaa aacacgacca tcatgcaaat gaagatgttt ttgacccgtc
    17941 ttggttttaa ttctaaaatg attgtcaatg gagatacgag tcagattgac ctccctcgaa
    18001 atgtcaagtc tggtttgatt gatgctactc agaaattaca aggcattaaa cagattgatt
    18061 ttgtctattt ctctgctaag gatgttgtcc gacatcctgt tgtggctgat attatcaagg
    18121 cttatgaaac gtcctcagag gagatgaagg acttactaaa acctaaggct agtgacgttg
    18181 aagctgatta ccagccaggc ttgacaaaat accctgtgat tggtcaagag cactaagatt
    18241 tttacgatga tgtttaatat ggccttttta aggaggattc tatggatgat ttaaccaaag
    18301 ctattatggc agatgaggct aatctctcct ataccgaaag aggtattttt cctttatatg
    18361 atgctcctca aacagctagg cttattattg ttggccaagc tcctggaata gtagctcaag
    18421 aaaccaaact ttactggaat gatcgtagtg gcatccgctt acgagactgg ctaggtgtgg
    18481 acaatgacac cttttaccac tcaggattgt ttggcattat ccctatggat ttctattatc
    18541 ctggtaaggg aaaatccggt gacttaccac ctagagaagg ttttgcggct aagtggcacc
    18601 cgccattaag agcattgatg ccagaagttg aattgacgat tttggttggt cgctatgctc
    18661 aggactttta ccttggtaac aaggcttaca aaaccttaac tgaaacagtc cgtcactttg
    18721 aggattactt gccagactat tttccacttg ttcatccttc tccaagaaac caattgtggt
    18781 tagctaagaa cccctggttt gagcaggatt tattacctat cttgcaaaaa cgtgtggagg
    18841 caattctaac caaataggcc ctgatttacc tagcattccc attttttagg ggcttgtggt
    18901 ataatgggta aataacaaat gctgattgaa aggataaggg aaattatctg tcagataata
    18961 atgggtgccc aacataccaa atgaggtggg cagcccttcc ctaacaatta accttatgta
    19021 tatcgagatg attgacgaaa cgggacaagt ttcgcaagag attatggagc aaacacttga
    19081 tttgctcaat tttgctgctc aaaagacggg caaagaagaa aaagaaatgt ctgtgacctt
    19141 tgtgaccaat gaaagaagtc atgaactcaa cttagaatac cgagatactg atcgtccaac
    19201 agatgtgatt tctctagaat ataagccaga aacgcctatt ttatttagtc aagaggattt
    19261 tgctgctgat cctagtttgg cagagatgat ggcagagttt gatgcttata ttggcgaatt
    19321 gtttatttct attgacaagg cgcgtgagca gtcccaagaa tatgggcact cttttgagcg
    19381 tgaaatggga tttttagctg ttcatggatt cttacatatt aatgggtatg atcactacac
    19441 ccttgaagaa gaaaaagaga tgtttacctt acaggaagag attttgactg cttatggcct
    19501 tacacgacaa taataccacc aaacggaaat ggaaaaacag aaccattacc tcaagcctgg
    19561 agtttgcctt gacaggggtt ttcacagctt ttaaggaaga acgcaatctc agaagtcacc
    19621 ttttgtctgc ctgtttagct tgtgttgctg gtttattttt tagcatttca gccatcgaat
    19681 ggttattttt acttctagcc atttttttgg tcattacctt agaaattgtc aattctgcta
    19741 ttgaaaatgt agttgatttg gctagtgact atcacttttc tatgctagct aaaaatgcca
    19801 aggatatggc agcaggtgct gttttgatga tttctggcta tgctgtcttg acaggactta
    19861 ttatttttat tcctaaaatt tggaatattt tcgttcatta aaaagaaagg aatgggctat
    19921 cctatgtgat gatggcatag gagaataagg aaaggacaag acggttaagc ttagtgacaa
    19981 gcttgatctg aagccctacc gttaaggccc gtaattatct atgtttaaat caggctttgt
    20041 agccattctt ggtcgtccaa atgttggcaa atccaccttt ttaaatcacg tcatggggca
    20101 aaaaattgcc atcatgagtg acaaggctca gaccacccgt aataaaatta tgggcattta
    20161 caccacagaa acagaacaaa ttgtctttat tgatacgcca ggtattcata aaccaaagac
    20221 cgcccttggt gattttatgg ttgaatctgc ttatagtact ttacgtgaag ttgagacggt
    20281 tcttttcatg gttcctgccg atgaaaagcg tggtaagggt gacgacatga ttatcgagcg
    20341 gctaaaagca gctaagattc ctgttatttt agttatcaac aaaattgata aggttcatcc
    20401 agaccaactt ttggaacaga ttgatgactt ccgtagccaa atggatttta aagaagtggt
    20461 cccaatttct gccctagaag gtaataatgt gccaacccta atcaagttat taactgataa
    20521 tcttgaagaa ggtttccaat attttccaga agatcagatt actgatcatc cagaacgttt
    20581 ccttgtgtca gaaatggttc gtgagaaggt actgcatttg acgcaacaag aggttccaca
    20641 ttccgtagct gttgttgtcg aatccatgaa gcgagatgaa gagactgata aagtgcatat
    20701 ccgtgcaacc attatggtgg agcgcgatag ccaaaaaggc atcatcattg gaaaacaagg
    20761 agccatgctc aaaaaaatcg gaaaaatggc gcgccgtgat attgaactca tgttaggtga
    20821 caaggtctac ttagaaacgt gggtcaaagt caagaaaaac tggcgggata aaaagcttga
    20881 tttagctgat tttggctata atgagaaaga atactaacat agtaaagcga gatcatatgc
    20941 cacaagacta catttcttat atccgctcca aagttgggca tgataagatt attcttaact
    21001 ttgctggtgg gattttgacc aatgatgacg gcaaggtgct gatgcagttg cgtggtgata
    21061 agaaaacctg ggctattcct ggtggtacta tggaactggg tgagagctct ttagagactt
    21121 gcaaacgtga gttcttagag gaaaccggaa ttgaggttga agctgttcgc ttactgaatg
    21181 tttacactca ttttgaggaa gtttatccca acggtgatgc tgttcagact attgtcttta
    21241 tctatgaact aacggctgtt tctgatatgg ctattgataa ctttcataat gaggaaacgc
    21301 tcaagttaca gtttttctct catgaggaga tagcagagtt agagagtgtc tcagccaaac
    21361 atcgcctgat gttagaggaa tattttagtg atagctttgc tatggggcat taagaactct
    21421 ctaggacagt tggagtattt ttggagattg gcttgttaaa aaaatttttg aaagacgatg
    21481 tttcgagagc ttatgctctc tttttttgct tccaattttt ctgattaagt ctgaataatt
    21541 ttgttaaatg ttaacaggat tttctttcca attgatacta gcagacatca aacatttgtt
    21601 ttagagttat gacgaattgc cacctagcca tgcatgactt aggcaagttg aacctaactg
    21661 tggcaataga cttggaaggg gtgacaagaa tcatttccta aaagaattga tgaagtgcct
    21721 atccattatt cgaattgtaa agttcgtcta agtaaagttg aatttgatct cgaatggact
    21781 ttaatgattt atgacttgtt gtgtc
//
LOCUS       DAUVUY010000024        21118 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.024, whole genome shotgun sequence.
ACCESSION   DAUVUY010000024 DAUVUY010000000
VERSION     DAUVUY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21118
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.024"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..369
                     /locus_tag="VOX58_000796"
     CDS             <1..369
                     /locus_tag="VOX58_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HES5878043.1"
                     /translation="LQTIYATFGYYNERQIALELEGIEGQKRIARIMEDFRQTPIASV
                     AEMALDKTIDFIDGYQDFPKQNCLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQEN
                     SATKLDAIEAACRTKINQVN"
     gene            577..1431
                     /locus_tag="VOX58_000797"
     CDS             577..1431
                     /locus_tag="VOX58_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HES5878044.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            1584..2924
                     /locus_tag="VOX58_000798"
     CDS             1584..2924
                     /locus_tag="VOX58_000798"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HES5878045.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHSLANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            2902..3117
                     /locus_tag="VOX58_000799"
     CDS             2902..3117
                     /locus_tag="VOX58_000799"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HES5878046.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            3117..3989
                     /locus_tag="VOX58_000800"
     CDS             3117..3989
                     /locus_tag="VOX58_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HES5878047.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            3982..4809
                     /locus_tag="VOX58_000801"
     CDS             3982..4809
                     /locus_tag="VOX58_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HES5878048.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVLASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQEVVAKAHKEFEKHEKE"
     gene            4796..5266
                     /locus_tag="VOX58_000802"
     CDS             4796..5266
                     /locus_tag="VOX58_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HES5878049.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            5288..6949
                     /gene="recN"
                     /locus_tag="VOX58_000803"
     CDS             5288..6949
                     /gene="recN"
                     /locus_tag="VOX58_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HES5878050.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            7121..8062
                     /locus_tag="VOX58_000804"
     CDS             7121..8062
                     /locus_tag="VOX58_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HES5878051.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFISQNETDKMFENLTDDRLLNKL
                     DELDELDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLR
                     SLSDTALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAH
                     YEDRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAVAK
                     RKIALPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            8278..9129
                     /locus_tag="VOX58_000805"
     CDS             8278..9129
                     /locus_tag="VOX58_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HES5878052.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            9122..9964
                     /locus_tag="VOX58_000806"
     CDS             9122..9964
                     /locus_tag="VOX58_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HES5878053.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLTKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            9942..10529
                     /locus_tag="VOX58_000807"
     CDS             9942..10529
                     /locus_tag="VOX58_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HES5878054.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            10628..10903
                     /locus_tag="VOX58_000808"
     CDS             10628..10903
                     /locus_tag="VOX58_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HES5878055.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            11257..13119
                     /gene="cadA"
                     /locus_tag="VOX58_000809"
     CDS             11257..13119
                     /gene="cadA"
                     /locus_tag="VOX58_000809"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HES5878056.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAETASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYALLDDIKI
                     ESKRAIKSLHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(13419..14354)
                     /locus_tag="VOX58_000810"
     CDS             complement(13419..14354)
                     /locus_tag="VOX58_000810"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HES5878057.1"
                     /translation="MVSTATQIGHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            14593..15288
                     /locus_tag="VOX58_000811"
     CDS             14593..15288
                     /locus_tag="VOX58_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HES5878058.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            15395..15916
                     /locus_tag="VOX58_000812"
     CDS             15395..15916
                     /locus_tag="VOX58_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HES5878059.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
     gene            16077..16619
                     /locus_tag="VOX58_000813"
     CDS             16077..16619
                     /locus_tag="VOX58_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878060.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            16743..17522
                     /locus_tag="VOX58_000814"
     CDS             16743..17522
                     /locus_tag="VOX58_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HES5878061.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFATNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            18320..18916
                     /gene="recR"
                     /locus_tag="VOX58_000815"
     CDS             18320..18916
                     /gene="recR"
                     /locus_tag="VOX58_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HES5878062.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            19017..20063
                     /locus_tag="VOX58_000816"
     CDS             19017..20063
                     /locus_tag="VOX58_000816"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HES5878063.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            20262..>21118
                     /locus_tag="VOX58_000817"
     CDS             20262..>21118
                     /locus_tag="VOX58_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HES5878064.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVG"
BASE COUNT         6728 a         3564 c         4424 g         6402 t
ORIGIN      
        1 ttgcagacta tttatgcgac atttgggtat tataatgaac gtcaaattgc cttggagctt
       61 gaggggatag aaggacaaaa acgcattgcg aggataatgg aagatttcag acagacacca
      121 atagctagtg ttgcagagat ggcgttagac aagacaattg attttattga tggctatcaa
      181 gattttccaa agcaaaactg tctcaaattt tatttggatg atggttcttg gtatgcactt
      241 agaccatcag gaacagagcc taaaataaaa ttttatttat atactattgg ccaaacacaa
      301 gaaaatagtg caacaaaatt agatgctatt gaagcagcgt gtcgcacaaa aataaatcaa
      361 gttaattaaa catcaaaaat ggataacact tttacactat ttttccatat aaacaactac
      421 aaaaggaggt gagactttta ggcaccttct ttttcttgta ggtgccacta atcaatttaa
      481 tcgactcaaa ccaatactaa aaaataagta agagtataaa gaacaggcat ctattttttt
      541 gttataatag aaaaatgaaa ttaaagggag atgtcaatga cagaactaat tgatggtaaa
      601 gccttagctc aaaagatgca acaagagtta gcagctaaag tcaacaatct aaaacaaaaa
      661 aaaggaattg taccaggctt agccgttatt cttgtaggtg atgatcctgc tagtcaggtg
      721 tatgtccgta ataaagagcg tgcagctctt actgtaggtt ttaaaagtga gaccgttaga
      781 ttatcagaat tcatttgtca agaagagctt attgcagtaa tcgaacgtta caatgcagat
      841 aacactattc atggtatttt agtgcagcta cccctgccaa atcatattaa tgataaaaaa
      901 attattctcg ccattgatcc caaaaaagat gtggacggtt ttcacccgat gaatacaggt
      961 cacctttggt caggacgtcc cttaatggtt ccttgtactc catcagggat tatggaattg
     1021 cttcgagaat ataatgttaa ccttgaaggt aaacatgccg tcattattgg cagatcgaat
     1081 atcgttggaa aaccaatggc acagctctta ctagacaaaa atgcaacagt cacgttgaca
     1141 cattcaagaa cacgtcaatt agaagaagta tgtcgctgtg cagatgtgtt gattgtggca
     1201 atcggacaag gtcatttcat aacaaaacaa tatataaaag atggtgcgat agtgattgat
     1261 gtgggaatga accgtgatga taatggcaag ctaattggag atgtggcctt tgatgaggtg
     1321 gcagaagttg cagcgaaaat cacccctgta ccaggaggtg tcggtcctat gacgattgct
     1381 atgttgctag agcaaactta tcaatctgcc ctccgtagta ctcataaata gttataatta
     1441 gacaattcaa cataagagaa tataagaagt cagcaatggc ttcctttttt gtttttctgc
     1501 tcactattta tccagaaaaa tggtataatt gcattagtat gcaaagggac tatgtcaggt
     1561 ccaactaagt gaaaggtggc ttgatggcag attatttaac cgtcactcat ttgacgaaat
     1621 atttaaaatt aaaatttgac cgtgaccctt atttggaacg ggtttatctg actggtcaag
     1681 tgtccaattt tcgaaaacga ccaactcatc aatatttttc cttaaaagat gaaagtgctg
     1741 tgattcaagc caccatgtgg gcaggagtct ataaaaaact aggattcgac ctagaagaag
     1801 gcatgaaaat taatgtaatt gggcgagtcc aactttatga acctagtggc tcttactcta
     1861 tcgtgattga aaaggcagag ccagatggta taggtgcctt ggctttgcag tttgaacaat
     1921 taaagaaaaa attaacggca gaaggttatt ttgagcaaaa acacaagcag cccttgccac
     1981 agtttgtatc aaaaattggg gtcattacaa gtcctagtgg tgctgtgatt cgagatatta
     2041 tcacaaccgt ttcacggcgt tttccagggg ttgagatttt attattccca actaaagtac
     2101 aaggtgatgg agccgcccaa gaagtagtgg ctaatattcg aagagccaat caaagagagg
     2161 atttggactt gctcattgtc ggccgtggag gtggctcgat agaagacctt tgggccttca
     2221 acgaggaaat agtggttcag gctatctttg aatcgcaact tccagtgatt tcgagtgtgg
     2281 gtcatgaaac agacacgact ttagcggatt ttgtggcaga tcgcagagca gccacaccaa
     2341 cagcagcagc agagttagca acgcctatta caaaaacaga tcttatgtct tggatagtag
     2401 agcggcaaaa tcgttcctat caggcctgct tgaggcggat taagcaacgg caagagtggg
     2461 tggataaact ctcgcaatct gttattttta ggcaaccaga acgattatat gatgcctacc
     2521 tgcaaaaaat tgatcgcctt agcatgaccc tgatgaatac gatgaaagat cgtctgagtt
     2581 ccgccaaaga aaacaaggtt cagttggacc attccttggc caatagtcaa ttacagacaa
     2641 aaatcgagcg ataccaagac cgtgttgcta ccgctaagcg tttactgatg gctaatatgg
     2701 ctagtcaata tgatagtcag ctggctcgct ttgaaaaagc acaggatgct ttgctatcac
     2761 tggatgccag ccgaattatc gcccgtggtt atgcaatgat tgaaaaaaat caagcactgg
     2821 tagcctctgt aagtcagata acaaagggtg atcagttaac cattaagatg cgtgatggac
     2881 aattagatgt agaggtaaaa gatgtcaaaa acgaaaacat ttgaagaaaa tttacaagat
     2941 ttagagacta ttgtgaacaa acttgaaaat ggggatgtcc ctttggaaga agctatttca
     3001 gaatttcaaa aaggaatgct tctctcaaaa gaacttcaaa agaccttaca agcggcagaa
     3061 aaaacactcg tcaaagtgat gcaagctgat ggcacagaag tagatatgga tgattaatgg
     3121 acaaattagc tagaattgac gaagccattc gtcgctacta taagacgact agtaacggtg
     3181 tatctgaaga gttgattgat gctatcttgt attctgttga cagtggtggc aagcgcattc
     3241 gcccacttat cttattagag atgattgagg gatttggggt atcgttacag aatgctcatt
     3301 ttgatttggc tgcagccctt gaaatgattc atacaggaag tctgattcac gatgatttgc
     3361 cagccatgga taatgatgat taccgacgtg gtagactgac caatcacaaa caatttggag
     3421 aggctactgc tattttggcg ggagatagct tatttttaga cccctttggg ttaatagctc
     3481 aagctgaatt aaatagcgag gttaaagtag ccttaatcca agaactttcc ctagcttcag
     3541 gaacttttgg catggttggt ggtcagatgc tagatatgaa aggggaaaat caagctttga
     3601 gtctccctca gttgtcactg attcacctca ataaaaccgg aaaattatta acttttcctt
     3661 ttaaagcagc agctcttgta acagaacagg ctattactgt tcgtcaacaa ctagagcagg
     3721 cgggaatgct catcgggcac gcctttcaga ttagggatga tattttagac gtgacagcta
     3781 gctttgaaga tcttggaaaa acgcctaaaa aggatttatt tgcagaaaaa gctacttacc
     3841 caagtttatt ggggttagaa gcctcctacc aactgttgac agagagttta gatcaggctt
     3901 tgacgatttt tcagacactc gaaagcgatg taggctttaa gcctcaaata attacaaaac
     3961 tgatagaagg gttacgactt aatgcctaaa gaaagagtag atgtgttagc ctataagcaa
     4021 ggattatttg agaccagaga gcaagctaag cgtggtgtta tggcaggctt agtggtctct
     4081 gtgattaatg gccaacgcta tgacaagcca ggtgacaaaa ttgacgatgg gactgagtta
     4141 aaacttaaag gtgaaaaact caaatacgtc agtcgtggtg gattaaaact ggaaaagggg
     4201 ctgcacgttt ttggtgtatc agttgctaat caaattggga ttgatattgg cgcttcaaca
     4261 ggtggcttta ccgatgttat gttacaagat ggggccaaac tagtctatgc cgtcgacgtt
     4321 gggactaacc agttggtatg gaaactcaga caagatccac gagtaagaag tatggaacag
     4381 tataactttc gttatgctca gccagaggac tttaatgagg gacagcctgt attggccagt
     4441 attgatgtgt cctttatttc tctcagcttg attttgccag ccctgcataa cgttttgtca
     4501 gatcagggac aggttattgc tctcattaaa ccgcaatttg aagctgggcg cgagcagatt
     4561 ggtaaaaaag gcatcgtcaa ggacaaacag attcatgaaa aagtgattca aaaggtcatg
     4621 gattttgcct caggttacgg atttacagtt aagggacttg atttttcccc tattcagggt
     4681 ggtcatggca atatcgaatt tttagctcat cttgctaagt cacagacacc tgaaacgtta
     4741 gccccgcatt tgattcagga agttgttgcc aaggcacata aggagtttga gaaacatgaa
     4801 aaagagtgaa cgacttgaat taatcaaaaa aatggttttg acgcatccaa ttgagacgca
     4861 acatgacctc ttaagattat tggcagagca tgggttagaa ttaacccagg ctactatttc
     4921 aagagacatg aatgaaattg gtatagtaaa gattccttct ggcagtggac gttacatcta
     4981 tggtctttct caagatagtg gaaaaaagat cgttcaagga cctagatcga taaagagcac
     5041 tattttagct gtatctgaca aaacaaaagg tttagaacaa cacctctatt taaaagtcgt
     5101 acctggcaat agtaaattaa tcaaacgtta tttattagca gatttttcaa aagctatttt
     5161 tagtcttatt gctgatgatg atagtttatt gttaattgca aagtctcctt cagaagcaga
     5221 tatgatacgc caagaaattt tgctttggat gcagggtata acctagaaaa aaatagggga
     5281 gaaagtgatg ttattagaaa tttctattaa aaattttgct attattgatg aaatttccct
     5341 aaattttgaa aatggtatga cggttttgac gggtgaaact ggagcaggaa agtctattat
     5401 cattgatgct atgaatatga tgcttggtgc gcgtgctagt acagaagtga ttcgtcgtgg
     5461 agctaataaa gcagaaattg aggggttctt ttcggtagat gctaccccag aactggttgc
     5521 gtgtctggaa tcatcaggca ttgctatgga agaagaactg attattcgcc gagatatctt
     5581 tgccaatggc agaagcgtga gccgcattaa tggtcagatg gttaacctag ccaccttaaa
     5641 acaggtcgga cagtttttgg tagatattca tgggcaacat gaccaagaag aattaatgcg
     5701 accacagctc caccagcaaa ttttggacgc ttttggtgat aaggcttttg agcaattgaa
     5761 agagaactat cagcttattt ttgatcgtta taaaagcttg cgtcgccagg ttattgacaa
     5821 acaaaaaaat gaaaaggaac acaaagatcg tattgatatg ttggcctttc agatagcaga
     5881 aattgaagct gctgccttga gtcgaggtga ggacgaccgg ttaaatcaag aacgtgatcg
     5941 cttaatgaac cacaaacaaa ttgctgatac cctgaccaat gcctacgtca tgctagataa
     6001 tgacgatttt tcaagtctat ccaatattcg ctctagcatg aatgacttac tatcaattga
     6061 gcagtttgat tcagagtaca aagggatgtc gacttcgatt tctgaagcct attatattct
     6121 tgaagaagtg agcaagcaat tatcagatac cattgaccaa ctggattttg atggtgggcg
     6181 attacaggaa attgaatttc gcttggatac actcaatagc ttaacccgta aatacggtgg
     6241 taatgtgaat gatgtgcttg actactacga taatattatc aaagaatacc agctattaac
     6301 aggagacgac ttgtcttcag gggatctaga atcagaattg aaagccttag aaaaacaatt
     6361 ggttgctgca gccactaact tgagtgaacg ccgccatcaa ttggcagagc aattagaagc
     6421 tgagattaaa gctgaactta aggaacttta tatggaaaaa gcagatttca aagttcactt
     6481 taccacatca aaattcaatc gtgatggtaa tgaaagcttg gaattttaca tctctactaa
     6541 tccaggcgaa ggatttaagc cccttgtcaa ggtggcttct ggaggagagt tatcacgcct
     6601 tatgttagcc atcaaggctg ctatttctag aaaagaagat aagacgagta ttgtttttga
     6661 tgaagtagat acaggtgttt ctggtcgcgt tgcgcaagcc attgcccaaa aaatttataa
     6721 aattgggcga catgggcaag ttctcgctat ttcacactta cctcaagtta ttgctattgc
     6781 tgattatcag tattttattt ctaaagaaag taaagaagag tccaccgttt ctaaggtaag
     6841 gttattaact cctgaagaac gtgtggaaga aattgctagc atgattgcag gaacagatat
     6901 gacacaagct gctcttacgc aggctcgtga actacttgcc aaacattaag attgtgtttg
     6961 gtatttgata ttttcataat tagatgacat tgatttttta ggtgctaaaa taatcattgt
     7021 gctagaccat ttttcctctg ttaatctggc ttggtttgcc ttgacaaacc tctgagaaat
     7081 agtagaatag ataagagaat tttaagctga ggggatagaa atgactagta aaaaagcgtg
     7141 tttatcaagc atcattgtgt tagcaagttt aacgtgtgga aatgatactg ttagtgccaa
     7201 tcatctctca gcaactggag ataagtttga tgattgctca acacttgttg aaaaagatgt
     7261 ggcccctaaa gatgaacttg agatgttagc atggtcctcg tctcaaacaa ctgatgatgc
     7321 tgacagagac tatgaagatt ttctcgatga tgattctttt atttctcaaa atgaaactga
     7381 taagatgttt gagaatttaa ctgatgatag gttattaaat aaattagatg aattagatga
     7441 attagatgaa ttagatgaag aagatacaat tgagccagag caaaatgtaa taatgcctag
     7501 tgacgatgag ctatttgatt taactgatgc tgttgagaca cgccttactg tttctagtgc
     7561 tccccattta gaggctgaat tgccgaaacc acatttgagg agcctatcag atacagcact
     7621 gcggtctggt gaaattagag gacatttaga taacaaactg gacgctttgt ctgtaacagc
     7681 tacaaagtta gcattaacga tggctcaaaa atttgatttg acaacgcatg tctattctat
     7741 aggtgaaagc tttagtgaag tattagctgc tcattatgaa gacagaaaag cagaatcagc
     7801 tttttctaag aaaaagagat ttcaccttcc tattgctact ccagatgttg ttatagagga
     7861 gttaaggcgc ctagtctctt ctattggaag ttcaaaagaa gatgtttcag ttccttataa
     7921 tcggaagcta ggtatggcag ttgcaaaaag aaaaatagcc ctgccacaaa cgggagagga
     7981 gttctcttat tatccagctt tacttggttt aatgatatta ggattaacgc cgattatgat
     8041 accaaagaag ataaataatt aggtaataag ttattgtagt tgtaaaagac ggggcaaatg
     8101 ttcaagcgtg aaaaggttga cttttgcctc gttttgtatc tcgttattgc taaggcattt
     8161 tcttcgagca gctagtgctc gccataccta tctaccgagt gattattcaa tagcactacc
     8221 agttttgctt tatttagtga ggagtgttag aataaagagg tgagaaacta gtttagaatg
     8281 gaaaaataca tgggaactat aaaaattgtt acagattcat caataactat tgaaccagaa
     8341 ttaattaaag ctttagatat tactgtagtg cctttatctg tcatgattga tagcaaatta
     8401 tattctgata atgatttaaa agaagaaggt cattttttga gtcttatgaa ggctagcaaa
     8461 tcccttccca aaaccagtca gccaccggtt ggtctctttg ctgaaactta cgaaaatttg
     8521 gtcaaaaaag gagtaactga cattgttgct attcacctct caccagcttt atcaggtact
     8581 attgaagctt cccgtcaggg ggctgaaatc gctgaggccc ctgttacagt tttagattca
     8641 ggatttactg atcaagccat gaaatttcag gtcgttgagg cagcaaagat ggcaaaagcg
     8701 ggtgctagtt tgaatgagat tttggctgct gttcaagcta tcaaatcaaa aacagagctc
     8761 tacattggtg tatctacctt agaaaattta gtaaaaggag gtcgtattgg tcgtgtgacg
     8821 ggtgttttga gctcgctact taatgtcaaa gttgttatgg cacttaaaaa tgatgagtta
     8881 aaaacgcttg tcaaaggtcg aggtaataaa acatttacga aatggttaga tagttacctc
     8941 gctaaaaata gtcatcgccc gattgcagag atagctattt cttatgctgg tgaagctagc
     9001 ctggcattaa ccttaaaaga aagaatcgca gcttattaca accactctat ttcggtttta
     9061 gaaacaggct ctatcatcca aacacataca ggagaaggag cttttgcggt tatggttcgt
     9121 tatgaataat cgtcatttat ttagtgggat atttttcttt gttattagtt tatgtctggc
     9181 ctttttattg ctaaatatta ttatccctaa gtcaaattca cgtttgaaaa agagtgattt
     9241 tctgacaaaa gaacaagtag ctatccaata tgttgctata ggagattcat tgacagaagg
     9301 agtaggtgat ctaactcatc aaggtggttt tgttcctttg ttaacgaatg atctcagtga
     9361 atattttaag gctaatgtta atcatcaaaa ttacggcgta tctggtgata ccagtcaaca
     9421 aattcttgat aggatgataa aacaaaagca gatacagtta tctttaaaaa aagcagatat
     9481 aatgacgtta accgttggtg gtaatgatgt tatggcagtt attcggaaga atttagcgga
     9541 tttgcaagtt tctagtttta gaaagccagc tcgtcagtat caaaaacgat taagacagat
     9601 tatcgagtta gccagaaaag ataataaaga tcttcctatt tttattttag gcatctataa
     9661 tccgttttat ttgaattttc cagaactaac tgatatgcaa aaagtgattg atgactggaa
     9721 taccaaaact aaggaggttg ttggagaata cgatcgtgtg tactttgtgc caataaatga
     9781 cctcttgtat aaagggataa atggacaaga aggaattgtt cattcttcag gagatcaaac
     9841 tacaattgtc aatgatgcct tgtttactgg ggaccatttt cacccaaata atactggcta
     9901 tcaaatcatg tcaaatgcag taatggagaa aataaaaaag catgaaaaaa aaatcaaacc
     9961 ttaattggtg gaagtggagc ttcttatgtc tgcttgcttt taataccgcc tttttgatgg
    10021 ttattgcaag tcgtctcatt caagtaagag aacctgagtc agaactgatt gctaaaaaac
    10081 cagttaaaaa tatcaaaatt ggtacatttg tcacaaccag agaacagctt aacgaaactg
    10141 tagcaagtta tcttaaagat tatcagacta aaaaaatgtc ttataaattt tatgcgacat
    10201 cttcttctat tttgtttgaa ggaacttatc aattattagg gtatgaagtc cctttatata
    10261 tctattttca acctcatcgt ctagaaaatg gagctgtcca actacaggtg atctcttttt
    10321 cagtgggaac tctgccactc ccagaaaaag atgttttgca gtatttgaaa tcaagctaca
    10381 aattgccaag ctttgttaaa gtgatgccaa atcaatcagc tatcgttgtt aatttacaag
    10441 atatccaaaa tgatgctaag gtttatttaa aggccaagaa aattgattta tttaatgatg
    10501 aaatcagttt taacatctat aagaaataag ctcaaataac gttaaatact tgacctaatc
    10561 gcttttttta gatatgataa tatctgttaa agcattaagc ttactaatta tttggaggat
    10621 ttgttaaatg gctaacaaac aagatttaat cgcaaaggtt gcagaagcaa ctgaattgac
    10681 taaaaaagat tcagcagcag cagttgatgc cgtgttttct acaatcgaag ctttccttgc
    10741 tgaaggtgaa aaagtacaat tgatcggttt tggtaacttc gaagtacgcg aacgtgcagc
    10801 tcgtaaaggt cgtaacccac aaactggtgc agaaattgaa attgcagctt caaaagttcc
    10861 agccttcaaa gctggtaaag ctcttaaaga cgctgttaaa taattcgtct agaaaaatct
    10921 tgttgctatc gatgtttatt gatagcgaca aggttctttt ttatatttgg ggcatatttg
    10981 gggcataaag ttagtagttt tctcttcagt taagccaagt tctaatgcat gcactctata
    11041 atggtcagtc atccttctat tagtatatca tttccgcagg tagggggagt ttttttcata
    11101 tttgcaaata gtcgaagtta ggatttttga aagcaaaagg taagtttaaa aaagtttatt
    11161 tagaatgctt ctaaattact tgacttattt atttagaatt attataatta atgtatctta
    11221 aagattgaat aacttttaat tgcgagggaa ctaaaaatga caatcagaca atggatggca
    11281 gaccatcttc atttaatgga gactttagcg tgtttggtat taataataat tggcctagcc
    11341 tttttacact catttccaca agtggcttcc gctattttca ttacagcctt cttgattgga
    11401 ggatatgcgt ctgcaaaaac gggtatattg gatttggtga agaacaaaca cttgtcagtg
    11461 gatattttga tgattttagc agctatcggt gctggaatta tcggctattg gctggagggt
    11521 gctctgctta tttttatctt ttcgttgtcc aatacgcttg aagaaatggc catggaaaaa
    11581 agtaaggatg ctatttcagc cttgatgtcc ttgacgccag atacagctcg tcaataccaa
    11641 gaagatggcc atattttaga agttgagacc agatccttga gtgttggtga ccgtttacaa
    11701 gttcgtaaag gcgaagctgt tccaattgac gggcagttgc ttagtccttt tggtcaattt
    11761 gatgaatcta tggtcactgg tgagcctatc actgttgata aggcagaagg ccaggatctg
    11821 attgggggaa ccattaacca aggacaaacc atagatatgt tggttactat tgaaaatgac
    11881 gatactctct ttgccaagat tattaatctg gtggaatctg cccaagaaaa gaaaagcaaa
    11941 actgccacct ttatcgaaag cttagaagat ggttatgtca aatttgtgct cgtccttatt
    12001 cctgccttta tcctctttag ccactttgtg ctttcttgga cttggttggc tgctttttac
    12061 cgagggatga ttctcttaac agtagcttca ccatgtgccc taattgccag ttccacacct
    12121 gctagcttgg ctgctatttc tcgtgcagcc agaaagggat tgattatcaa aggaggggat
    12181 attgtcgata acatgggaga tattaaggct gttgtcatgg ataaaacggg aacgctcacc
    12241 caaggaaaac cttctgttgt gaatgctcat tatttggaag atgagttgct cgtgaataga
    12301 ctggtaaaag gagcagagac tgctagtacc catcctattt ctaaagccct tcttgaatac
    12361 actgaaaaat tggagccact gacctttgac cacttagaag aaatttctgg gaaaggtttt
    12421 cagggcttct atcaagggca agaatggcga attggcaaga aaaccttcat tttggaaaag
    12481 gttcaagacc tatcagcttt tgaagaaact attcaagtgg aagaaaatca agggaaaacc
    12541 ctaatctttg tttcacgtga ccatcaattg atagcttact atgccctctt ggatgatatc
    12601 aaaatagaat caaaacgtgc tattaagtct cttcatgcca tgggaatcaa aacagtcatg
    12661 ttaacaggtg accaagaacg aaccgccaat tatgtggcac aaaaacttgg tattgatgaa
    12721 gtggtagcca actgtatgcc tcaagataag gtggctaagt tagcagaatt aaagaccaaa
    12781 tatggttttg tggccatggt aggagatggt attaatgatg ctcctgccct tgctcaagca
    12841 gatgtttctt atgctattgg atcaggaaca gatattgcaa tggaaagtgc agacagtgtg
    12901 attatggatg acttgactcg tattccattt tcgattcaac tttcccgcaa aatgaagacc
    12961 attatcaaac aaaatattgt ttttgcctta tctgtgatta ccttattgat tttagccaat
    13021 gtttttcagg tagttaactt gccgcttggt gttgttggac acgaaggctc aacgatttta
    13081 gtgattttaa atggcttgcg tttactttct tttaaataat ttagaaactt gcttgaaaac
    13141 ttgaaagtgt ttataataat agtaagactt atgaaagaga aaaccgccgt atgtttacta
    13201 ttatgatcat tggtttagct gtattttcaa taattaaaat cattctattt ttctattttt
    13261 atcagcaatt tgagcgcaag caaaaaagaa aagttgaatc aaacaaccag caaagacagt
    13321 cccaagaaag tgacgctgtc ccttcaaaaa aattcccatt ttaaaaagct tggaaagtgt
    13381 acaaagtgca catttccaag cttttttgac tattaaggtt aatctttgta acgtaagttc
    13441 ccacgaaaat cgtctaagct ttggtagcct ttttctacca taatcgtttt tagttcctta
    13501 gtgacccgtt caaagatagc tggcccttct tgatgaaggg ctgtcccaat ttggaccatg
    13561 ctggcgccac aaagaatatg ttcaaaggca tcgcgtcctg ttttaactcc ccctgtacca
    13621 ataatgtgga tagaaggttt taagcgtttg taaaacgcat ggacattggc tagagcagta
    13681 ggtttgatgt aatctccacc tataccacca aagccatttt taggcttgat gaggacttgt
    13741 tcatcctcaa tgaccagccc atttccgata gaattgacac agttaacaaa ggaaaggggg
    13801 tatttattga aaatcgcagc cgcttgatca aaatgcacaa tgtcaaaata aggaggtaat
    13861 ttgataccaa gaggtttagt gtaataggta aagatgttct ctagaagttg atcagtggtt
    13921 tcaaaatcat aagcaatctg tggttttcct ggaacgttag ggcaagaaag gtttagttcc
    13981 actagaccct cataatctga agccatgatg gcttttaaaa tggtttcggt ttcagtagga
    14041 gatagcccga caacggagag aaagtgaggt ttatgttgcc cggttttggc aagatcactg
    14101 acaaagtcta agtagtagcg aaaaccatta ttagggagtc ccattgaatt gatagaacca
    14161 agacgagtat ctgcgtagcg aggttctgga ttacctggac gaacttcaag cgtccctgtt
    14221 ttggtgacaa aggaagcggc ttgagacttt tcaacctcca taagctcttc ttttgtcatg
    14281 cagtaaacac cagcagcatt catcaaacag ttatcaaaag agaaatgacc aatttgcgta
    14341 gcagtagaaa ccattatttc ctccatattt tagacaaatt ttttctattc taccactagg
    14401 catgaataat agcaagctat tttcacaaaa atgtaaatat attactgtta ttgacgaaca
    14461 tttgaaattt tcatgatagt cacattatga ttttcaataa agtcagaata gactagcttt
    14521 tttgctatat tttttgtaaa ataggaattg taaaaaggta gcaaccttta aattaaaagg
    14581 agatattaac tcatggtaaa attggttttc gctcgccacg gtgagtcaga atggaacaaa
    14641 gctaacctct tcactggttg ggcagatgtt gatctttcag aaaaagggac acaacaagcg
    14701 attgatgcag gtaaattgat caaagaagca ggtattgaat ttgaccttgc tttcacttca
    14761 gtattgacac gtgctatcaa aacaactaac cttgcccttg aaaatgcagg tcaattgtgg
    14821 gttccaactg aaaaatcatg gcgcttgaac gagcgtcact atggtgcttt gactggcaaa
    14881 aacaaagctg aagctgcaga acaatttggt gatgaacaag ttcatatctg gcgtcgttca
    14941 tacgacgtat tgccgccagc tatggctaaa gatgatgagt attcagcaca caaagaccgt
    15001 cgttatgctg atcttgaccc agctcttatt ccagatgctg aaaacttaaa agtaactctt
    15061 gaacgcgcaa tgccttactg ggaagaaaaa attgctccag ctcttcttga tggtaaaaac
    15121 gtctttgttg gcgcacatgg taactcaatc cgcgctcttg tgaaacacat taaaggtctt
    15181 tcagatgacg aaatcatgga tgtggaaatt ccaaacttcc caccacttgt ttttgaatta
    15241 gatgaaaaac ttaacattgt taaagaatac taccttggtg gtgaataact taaaaaccag
    15301 actttggtct ggttttttgg ttctatatcc taagaagaaa gaaaaccctg cttaaaaata
    15361 tgatatgata gaaataaaaa aggatagata gattatgaaa gcagaagatc gtcgtcaaaa
    15421 aattattgag tgcttaaata gtgaacaaaa agctgtatct gctactcgtt taggtaagtt
    15481 attaggagtg agtcgccaag taatcgttgg agatatagct cttcttcgag ctcagcagat
    15541 tgatattatt tcgactccaa aaggttatat tatgtcaaca gcgctttata gtcaccaatt
    15601 ttgtgcacgt attgtgtgcc aacataatgt agaagaaact aaaaaagaat tagaaattat
    15661 tttagctcat caagggatta ttacgacggt agaggtagaa catcctattt acggtatgat
    15721 aacagcccct ttaaatatta agacacactc tgatgtaact aatttcatgt ccaaactcag
    15781 tcaatctaaa gcagagttat tgtcttcgtt gacagaagga ctacatagtc acttaatttc
    15841 gtgtcctagt caagaagcct ttttagctat caagcatgat ttagaattag caggtatctt
    15901 atacaaagga atataactat agtatattat gccattgatg gttgttatga ataccatgct
    15961 aaaatattct attgattatt tatcagcaac aaggtatcgt ttaaaaatac caaaagatac
    16021 aggtgtcttg atagttattg gtttttaagg tatactattt ttgaaaagga gataaaatga
    16081 ctaaaaaacc aacacaacta atagcttata cctctatttt ggtcgcattt gcgattttga
    16141 ttccgataat catgcctctt aagcttatta ttggaccagc ttcctttact ctggcgagcc
    16201 atgttccttt gtttttggct atttttatgt cgattccagt agcaattttg gtcgctcttg
    16261 gtaccacatt aggtttttta ttagctggac ttcctttaat tattgtttta agagcactct
    16321 ctcatttatt gtttgctatt ttggcagcgt ggtggttaag tagaaaacca caactgatga
    16381 catcggcagt aaaatgtttt agttttgcgt ttttcattaa tgtcattcat ggcttggctg
    16441 aatttttagt tgtctatatt ttaacagcaa ccacagcaac ttctatgtct tatttttggt
    16501 ctatgctagg cttgattggc ttaggttcgc ttattcatgg catattggat ttctatcttg
    16561 ctttagttct atggcgtttt ttagcaaaaa acttaaaact tcccaccata aatgcctaag
    16621 actaaaatag agatagttat atgataatga cgagcccttt cttgttgaaa gggcttgcat
    16681 tttttaggaa aattgatata ctaaagtgtg tgtttgtgaa tacagtaaca aaaggagata
    16741 tcatgaaaac accagaacag attttagaag caactattca tattggggaa cataaggtta
    16801 ccaaaacttt tttggctaaa tctatcttag gctttattgg tggagcgatg attagccttg
    16861 gttatttact ctacgtgaga attgccgcca gtggtttaga aacttttggt gctttttcca
    16921 gtatcgtagg agcttgtgct tttccgattg gtctaattat tattttaatg gcgggtggag
    16981 aattaattac agggaacatg atggctgttt ctgcagcttt gttggccaaa aaaattaagt
    17041 ttagcgagct tgccaagaat tggcttatca ttaccttgtt taatgtgatt ggagctgttt
    17101 ttgtagcttt tgtgtttggt cattttctag gcttaacatc agctggtatt tttaaagaag
    17161 aagtgattga ggtggcccat gctaaaatag cagcgagccc tttgcaggca ctagtatcag
    17221 gaattggttg taactggttt gttggtttgg ccttgtggct atgctatggg gcaaatgatg
    17281 ctgctggtaa attccttggt acttggtttc ctgtcatgac ctttgtagcc cttggttttc
    17341 agcatagtgt agcaaatgct tttgtcattc ctgctgctat ttttgaagga ggagcaacat
    17401 ggttggattt tgccactaat tttatttttg tctatagtgg taatattatc ggtggagcca
    17461 tttttgttag ttttttatat tttaaagttt actatcatcc gcaaaagagt aaaactcaat
    17521 gagaaaatat ttttttagac tgagggcgtc ctattctcag tcttcttttt ataatttatg
    17581 gtaaaataag aagcgaagtt taaaaaattt agctaaataa tggttagttt attccctaaa
    17641 aaaggagtat tcccaaaaaa tccgtaaaaa gaatttgcta tgggagtagt tcccaaaata
    17701 atgcagatca ttagttgtct ttcaaaaata gccttgatag ggtaaaaagt ggacagtgat
    17761 gcagcaacag attcatcagg cgtacaaact gtcttatggt ttgtgcttag agatttctta
    17821 ataatgcttt tggtaataca cttttagtga agaaagtgta gcatgaaagc ttaaagataa
    17881 ccatgagttc gctttatgca ctcttgttga gaaaaatagc atagtcaatt gagtaaacat
    17941 catttctgca tggaatgaaa caattcctat aaacttatat cataaggatg gtttgcggtt
    18001 tctaaaaaag taaaatgaat caaaaaacaa aagccaaata tctcaaggcc aaggtaacaa
    18061 gtcgagtaaa aaagttttta cttggctaat ccaaacggtt ctgtcacgat tcacgctaaa
    18121 attggaactg ctgagacttg tatcacagac aattcagatc agaccttagt aacttacaac
    18181 ttaaatgtag tagcttataa tacaaataac gaagataata ttgcggttgc tgttatgtat
    18241 ccacatatta cgaatatatc ggcaaagatg tgagggcgct ttatgtggcg atatatggaa
    18301 aacaataaag gagaacttag tgctttatcc aacacccatt gcaaagttaa ttgacagtta
    18361 ctctaaactt ccaggaattg gtatcaagac ggcgacgaga ttagcctttt atactattgg
    18421 aatgtcaaat gaagatgtca atgattttgc taaaaactta ttagcagcta aaagagaact
    18481 gacctattgt tcgatttgtg gaaaccttac cgatgacgat ccttgtcaca tttgcacaga
    18541 cacgagccgt gatcagacga ccattctggt agtagaagat gctaaagatg tttctgccat
    18601 ggaaaaaatc caagagtatc atggctatta tcatgtgctt cacggcttga tttcgcctat
    18661 gaatggtgtg gggccagatg acatcaacct taaaagttta attacccgtc taatggatgg
    18721 taaggtgagc gaagttatcg tagctaccaa tgccacagca gatggggaag caacgtccat
    18781 gtatatttca cgtgtcttga aaccagccgg gattaaggta actcgtttgg caagaggtct
    18841 cgcagtaggt tcagatattg agtatgctga tgaagtaaca ttattgagag ctattgagaa
    18901 tcgtactgaa ctttaatgat catgttggtg cctttactcg caaagagagg ttttgaaaag
    18961 gctacatctt tttgttataa taaaaaaaca ttcaattcag ataagaaagg ttagctatgt
    19021 ccaaacaaac gttagttcta ttatacggag gccgctcagc agaacgtgaa gtctcagtgc
    19081 tttcagcaga gagtgtgatg cgagcagtca actatgataa gttcttagta aaaacttatt
    19141 ttattactca gatgggccaa tttatcaaaa ctcagcaatt ttcagaaaag ccatcggaat
    19201 cagaacgctt gatgacaaat gagacgatag agctaactca aaaaataaaa ccaagtgata
    19261 tctatgaaga aggtgcagtt gtatttcctg tattacatgg acctatggga gaagatggct
    19321 ctatccaagg tttcttagaa gtgcttagaa tgccatatat agggactaat gtcatgtctt
    19381 ctagcatagc tatggataaa attactacta aacgtgtctt agaatcaatt ggaattccac
    19441 aagttgccta tactgtttat attgacggac aagatttgga ggcttgttta gttgaaactt
    19501 tagcaagact tacttttcct atcttcgtaa aaccagctaa tatgggttct tctgtgggga
    19561 tttccaaagc ccaaacaaaa gtagaattaa gaaaagcgat tcagttagcc ctgacctatg
    19621 acagtcgtgt cttgattgaa caaggcgtgg ttgccagaga aattgaagtt ggtctccttg
    19681 gaaatgataa ggttaaatca actctaccag gagaagtaat caaggacgtt gatttttacg
    19741 actaccaagc aaaatatgtt gacaataaga ttacgatggc cattccagca gatgtagacc
    19801 agagtattgt gacagaaatg cgttcttatg ctgaagtagc ctttaaagcc ttaggtggtt
    19861 gtgggttgtc ccgttgtgac tttttcttga cccaagacgg acaggtttac cttaacgaat
    19921 taaacaccat gccaggcttt acccaatggt ctatgtatcc actcttatgg gaaaatatgg
    19981 gattggctta tccagatttg attgaagagc tggtgacact ggcacaagaa atgtttgacc
    20041 agcgtgagag ccacctgatt taaacctatg agatgaataa acattgactt ggatggaacg
    20101 gcaaacatcg tcaagatgtg acgttcgtcc tttttttgtt gctcttttag gaagaatagt
    20161 agagccatta agggagtttg acattttaga aatttttcta atgttttgtg ctataataga
    20221 taggacatcg taaatactag tgttcttaga aagaaaacaa aatgaaatta accttacacg
    20281 aagtggctaa aatagttgac gcccaaaata atgtgtctga cttagacgac gttcctctgc
    20341 atcacattga atttgatagc cgaaagatta caaaaggaga cctcttttta cccttaaaag
    20401 gtcaaaggga tggtcatgag tttattgatt tagcctttca aaatggagct gtcgctactt
    20461 tttcagaaaa agaattgccg gggaaacctc accttcttgt tgaagactgc ctaaaagctt
    20521 ttcaaaagct agcccattat tatattgata agatgcgcgt ggatgttatt gctgtgacag
    20581 gctcaaatgg taaaacttcc accaaagata tgataggagc tgtcctatca accacttaca
    20641 aaacttacaa aacgcaagga aattacaata atgagattgg tttgccttac accgttctcc
    20701 acatgcctga cgatacggaa aaaattgttc ttgaaatggg acaagatcac atgggagata
    20761 ttcgtcttct gtctgaaatt gcaaggcctc gtattgcagt tttaacctta gtcggcgaag
    20821 ctcatttaga atattttgga agccgagaca aaatagccca aggcaaaatg cagattgttg
    20881 acggcatgaa ttcagatggg attttaattg ctccgggaga tccgattatt gacccatatc
    20941 ttccagaaaa tcagatggtt atccgttttg gaaatcagca ggaaattgac gttactggta
    21001 ttcaagaaga caaagatagc ttaacgttta caacaaatgt gttagcaaca cctgtgtccc
    21061 tacctctacc aggaaaatac aacgcgacca atgctatggt agcagcctat gtaggaaa
//
LOCUS       DAUVUY010000025        20914 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.025, whole genome shotgun sequence.
ACCESSION   DAUVUY010000025 DAUVUY010000000
VERSION     DAUVUY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20914
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.025"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1067
                     /locus_tag="VOX58_000818"
     CDS             <1..1067
                     /locus_tag="VOX58_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878065.1"
                     /translation="KRLELLGIENGSINNGEINEPESQYIQIATALKKLDFIYIKPSS
                     VYIPDYMKNTNFAEDFFKKLNNDGITDPEVNKEMIYINKSGANIQNKVNGTDYQLLDN
                     KVATIYIPDKFKEKKKSIENTVVAEQFTGTKYTKENLAVQIIPNEKKIFYFNENGNEH
                     DENKEASPMANVTDSKGNIVVVLDTDKMVASKDFSLASNIVNSSLFSPEAIKKINDLS
                     LHLNFSINPVDVYQLVKLNIQSLKHQIFLSKVLQKIIYGIVFLLIYQYAQLFISSKQN
                     DYVKKIILGLSKTRIAISSLKYFIMTIAIVIICTFMVTEQIELLYIGIASLVVLILST
                     VMSFRKLSKKYTQILKGDEQ"
     gene            1064..1685
                     /locus_tag="VOX58_000819"
                     /pseudo
     CDS             1064..1685
                     /locus_tag="VOX58_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1682..2173
                     /locus_tag="VOX58_000820"
     CDS             1682..2173
                     /locus_tag="VOX58_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878066.1"
                     /translation="MKKKIYIGLIVIVGVFVVFLSGKQITQKETSNKPVVTLTVYTIS
                     SSDTQKWSKVKQFETKEAIYLVKVKETTSSEEVFSNIIADGMAVGFGVSEEDVKQFND
                     NLCSPVEDAKNNKLIGIEFINFSSVDKGFVVANFDYGKKAFNSQKTENKKLYKKLYEA
                     YKG"
     gene            2377..2907
                     /locus_tag="VOX58_000821"
     CDS             2377..2907
                     /locus_tag="VOX58_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HES5878067.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFLTRLFAK"
     gene            2956..3543
                     /locus_tag="VOX58_000822"
     CDS             2956..3543
                     /locus_tag="VOX58_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HES5878068.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            3696..4424
                     /locus_tag="VOX58_000823"
     CDS             3696..4424
                     /locus_tag="VOX58_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HES5878069.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTEGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKRFITLIVCLTVETLLFVGGAYF
                     LQLTVWKYLLIPVFLLVLLGTLYWYYQNICYLCPSCHQTFEAKWTEFALAGHTPRTRK
                     LTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(4481..4780)
                     /locus_tag="VOX58_000824"
     CDS             complement(4481..4780)
                     /locus_tag="VOX58_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HES5878070.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDEATKKTLGAFAGKLPF"
     gene            complement(4973..5347)
                     /locus_tag="VOX58_000825"
     CDS             complement(4973..5347)
                     /locus_tag="VOX58_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5878071.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLESKGVSYQLPQRELEMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
     gene            5517..5738
                     /locus_tag="VOX58_000826"
     CDS             5517..5738
                     /locus_tag="VOX58_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5878072.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(5818..8100)
                     /locus_tag="VOX58_000827"
     CDS             complement(5818..8100)
                     /locus_tag="VOX58_000827"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HES5878073.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKI
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDHYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYSHGLQDWNVKPRQVYEIFNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRASKAALFKGKANQALIDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(8122..8781)
                     /locus_tag="VOX58_000828"
     CDS             complement(8122..8781)
                     /locus_tag="VOX58_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HES5878074.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            8947..10143
                     /locus_tag="VOX58_000829"
     CDS             8947..10143
                     /locus_tag="VOX58_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HES5878075.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMVIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVLS"
     gene            10645..11493
                     /locus_tag="VOX58_000830"
     CDS             10645..11493
                     /locus_tag="VOX58_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HES5878076.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKVSYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(11829..12725)
                     /locus_tag="VOX58_000831"
     CDS             complement(11829..12725)
                     /locus_tag="VOX58_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HES5878077.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYLGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            13023..13775
                     /locus_tag="VOX58_000832"
     CDS             13023..13775
                     /locus_tag="VOX58_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HES5878078.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            13760..14710
                     /locus_tag="VOX58_000833"
     CDS             13760..14710
                     /locus_tag="VOX58_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HES5878079.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKAYYPSIVDATVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKALNHLTQKGNKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTIERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(14890..17217)
                     /gene="pflB"
                     /locus_tag="VOX58_000834"
     CDS             complement(14890..17217)
                     /gene="pflB"
                     /locus_tag="VOX58_000834"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HES5878080.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            17426..18520
                     /gene="dinB"
                     /locus_tag="VOX58_000835"
     CDS             17426..18520
                     /gene="dinB"
                     /locus_tag="VOX58_000835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HES5878081.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDVVEPMSIDEAYLDVTNNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGITMTNLEDKVADISLDL"
     gene            complement(18613..19095)
                     /locus_tag="VOX58_000836"
     CDS             complement(18613..19095)
                     /locus_tag="VOX58_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878082.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSILNVLAIIAIIVFCAQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTMSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(19186..>20914)
                     /locus_tag="VOX58_000837"
     CDS             complement(19186..>20914)
                     /locus_tag="VOX58_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HES5878083.1"
                     /translation="SPKRFRAALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQV
                     ECDPAAVAEQLSELIIEGKIKNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHD
                     TIQTTIQAVQKDFAITYDQVQQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANL
                     HQIDLDKKDLPILLAAPTGRAARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCD
                     LLIVDEFSMVDTWLANQLLGAINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQI
                     ALQKIFRQSQESTIVNLADQMRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSA
                     IKSGIPAEEIQILAPMYKGQAGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLH
                     LVNDAQLNVFNGDIGYITDLIPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAM
                     SIHKSQGSEFQVVILPITRQSGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEG
                     DKRQTYLIERFQEQSDLASSQSTNSNPTENSQSDNDDFRLTPENYSTIDSMIGLTESD
                     IALFFQKKS"
BASE COUNT         6571 a         3616 c         4210 g         6517 t
ORIGIN      
        1 ggaaaagact agagttgttg gggattgaaa atggctctat taataatggg gaaataaatg
       61 agccagaatc acaatatata cagatagcga cagccttaaa aaaattagat tttatatata
      121 tcaaaccctc ttcagtatat attccagatt acatgaaaaa tactaacttt gcagaagatt
      181 ttttcaaaaa attaaataat gatggtataa cggatccaga agtaaataaa gagatgattt
      241 atatcaataa atcgggcgct aatattcaaa ataaagtcaa tggaacagac tatcagctgt
      301 tagataataa ggtagcaaca atctatattc ctgataaatt taaagagaaa aagaaatcaa
      361 ttgaaaatac cgttgtggca gaacaattta ctggaactaa gtatacaaaa gaaaaccttg
      421 cagtgcagat aattcctaat gagaaaaaaa ttttttattt taatgagaat ggaaacgagc
      481 atgatgaaaa taaagaagcc tcacctatgg caaatgtaac agatagtaaa ggtaatatag
      541 tcgttgtgtt agatactgat aaaatggtag caagcaaaga tttttctttg gctagcaata
      601 tagtaaatag ctctcttttt agtcctgaag caataaaaaa aattaatgac ctgagtctac
      661 atttaaattt ttcaattaat ccagtagatg tttaccaact tgtaaaactt aatattcaat
      721 ccttaaagca ccaaattttc ctttcgaaag ttttgcagaa aattatttac ggcattgtat
      781 ttctactaat ttatcaatat gctcaattat ttatttcttc aaaacagaat gactatgtga
      841 aaaaaataat tttaggcctc tcaaaaacac gcatagcaat ttcgagtctc aaatatttta
      901 ttatgactat tgcaatagtt ataatatgta catttatggt gacagagcag attgagttat
      961 tatatattgg tattgcttca ttagtggttt taattctttc gacagtaatg agttttagaa
     1021 aattatcaaa aaaatacact caaattttaa aaggagatga acaatgacaa ttgaaatttt
     1081 caacgttaca aaaaaattca gctcaaaaaa aatatttaca gatttaaatt taacgtttga
     1141 agctggaaaa agctatgcct tgattggagg ttctggctct ggtaaaagta ctctgcttaa
     1201 cattatagga agactagaaa aaattgataa cgggaaagtt ttagttgacg aacaagatat
     1261 ttggaaaata aaagaaagaa ctttctttaa aaatattatc gggtatgtat tccagaatta
     1321 ttctttaata gaaaacaaaa ctgtatatga taatttgaaa ctcatcaata aagacaaaaa
     1381 aaatatctgc tgtacttgaa aaagtagggc tctctagtgg ctatttaaat caaaaaatat
     1441 atgaattgtc tggagggcag gctcagcgtg tagctattgc aagaatgtta atgaagccac
     1501 gtaaaataat tttagcagat gagccaacag gagctttgga tggtgaaatt ggaaaagaaa
     1561 ttattaattt gttgttgagc gaaacagccg aaaataaata cgttattata gcaactcatg
     1621 atccagctgt atataataag gttgatttta taattgatat gaaagatata gggagtaaga
     1681 aatgaaaaag aaaatttata ttggcttgat tgtcattgtt ggagtttttg tagtgttctt
     1741 gtctggaaaa caaatcacac aaaaagaaac ttcaaataag cctgtggtga ccctaacagt
     1801 ttataccatc tcctctagtg atacccaaaa atggagtaaa gtaaagcagt ttgaaacgaa
     1861 agaagcaata tacctagtaa aggtaaaaga aactacctct tcagaagaag tcttttcaaa
     1921 catcattgca gatggaatgg cagttggttt tggagttagt gaagaagacg ttaaacagtt
     1981 taacgataat ttatgtagcc cggtagaaga tgctaagaat aataaattaa ttggaataga
     2041 attcatcaat ttttctagtg ttgataaggg gtttgtggtt gcaaattttg actacggcaa
     2101 aaaagcgttt aattctcaga aaacagaaaa taaaaaatta tataaaaagc tctatgaagc
     2161 gtataaagga tagctattta tagttttttg agagtgatac cacttctgaa taataaatga
     2221 actaacatgg cataccgttt tctattcatc taacaatgtt ttcaagaaat aattgatgcc
     2281 tttagcacga ctgaaatgag gtcaaattga cagaatactc ctattatggt actattttta
     2341 ataagaatat tcagtgatag tgataaagag gaaaatatgg gaattgaaaa gacagttagt
     2401 gaactagctg atattttggg agtgagtcgt caggccgtca ataatcgtgt taagtcttta
     2461 cccgaagaag atcttgacaa aaatgaaaaa ggcgttactg tggttaagcg cagtggtctt
     2521 gttaagctgg aagagatcta taaaaaaaca atttttgacg atgagcctat cagtgaagaa
     2581 acgaaacaac gtgagctttt agagattctt gtggatgaga agaacactga aatcacgcgc
     2641 ctttatgagc aactcaaagc caaagatgct caacttgcct caaaagatga gcaaatgcgt
     2701 gttaaagacg tgcagatcgc tgaaaaagat aaacaattgg atcagcagca acaattaact
     2761 gctaaagcca tggctgataa agaaacccta aaactagaat tggaagaagc taaagcagaa
     2821 gccaaccaag ctcgcttaca agtcgaagaa gttcaggctg aagttggccc taaaaaaggc
     2881 tttttaaccc gcttgtttgc gaaataatca caaaaaatga cgaaggccct atcagggtct
     2941 tttttggagg aaaacatgaa acacttagat acttatattg cctttgattt agaatttaac
     3001 actgtcaatg acgttagtca tgtcattcaa gtatcagcag tcaaatatga ccaccataaa
     3061 gaagttgaca gctttgacac ctatgtttac acagatgttc ccttacagag ttttatcaat
     3121 ggcctaacag gaatcacatc tgacaagatt gctgcggcac caaaagtaga ggaggttatg
     3181 gctgcgttta aaaattttgt gggagagtta ccactgattg gttataatgc tcaaaaatct
     3241 gacctgccta ttctagctga aaatggctta gatttaaggg accaatacca gattgacctc
     3301 tttgatgaag cttacgatag acgtagcgca gacttaaatg ggattgctaa ccttcgtctg
     3361 caaacagtgg caaccttttt aggcattaag ggtcgaggtc ataatagctt agaagatgcc
     3421 agaatgacag cagcaatcta taagtcattt ttagaaacag atacaaacaa agcatatctc
     3481 agccagcaag aagaggtaac cacagataac cctttcgcag ctttgggaga cttttttgac
     3541 taagggctct gctatttcta aaaaataaca aaaattccta gggttagctc ttgcttgtaa
     3601 cgctaggtca tgtgataaga taggggcata tcaagaggga gtcgagtgca cacgaatcgt
     3661 ttttgatcac taaatgatag aaagaaggtg tggaaatgtc tcaattctat tcgactggag
     3721 aattggccaa cttagctggc gtttctattc gaactgtcca atactatgac caacgtggga
     3781 ttttgattcc gacagctttg acagaaggtg gacggcgact ctatacggat tctgatttag
     3841 aacaattacg catgatttgc tttctgcgcg atttgggatt ttccattgag cagattcgca
     3901 aagtcttagc agaggaaaat gcagcccagg tgctggaatt gcttctagtt gatcatattg
     3961 cgactgccaa ggaagacttg gccgcaaaag agcaacaagt tgatatagct gtcaagatac
     4021 ttgacagact tcgaaaacaa gacccccagt cactagattt tctgatggac atttcgctta
     4081 gcatgaaaaa tcaaaaagct tggaaaaaac gctttattac tttaattgtc tgtttgacag
     4141 tggaaactct actttttgtg ggtggagcct attttctcca attgacagtc tggaaatatt
     4201 tattaatccc ggtgttcctt ttagtcttac taggaacctt atactggtat taccaaaata
     4261 tctgttatct ttgtccttct tgtcatcaaa catttgaagc aaaatggaca gaatttgccc
     4321 ttgcaggcca tacaccacga accagaaagt taacctgctc ccattgccat caaaaatcgt
     4381 attgcttgga actggcaaaa gaggacactg tctcaagagg ataagcacta actcataaaa
     4441 aaactagcct gagatccttc tcaagctagt ttttgttttt ttagaatggt aatttaccag
     4501 caaaagcgcc taatgttttc ttagtcgctt catcaatttg cgtaagggca tcattaatgg
     4561 cttgagtggt catgtcttga agcgtttcta catcttcagg atctacaaca gcttctttga
     4621 agtcaatccc aaccaatttt ttatcacccg tgaaagtggc agtgacaagg tcttgagctg
     4681 attttccagt aaattgcata gcagccaaat ccgcctgctt ttgctccatt tgtttctgaa
     4741 gcttttgtgc ttgcttcatc atgttttgca tgttcatcat aagactatac tctccttaat
     4801 ttaatactaa ctattataac atataaaaag aggcaataaa aacaatgtca gttagtagac
     4861 tagcctgtat cagtaagaaa gtcgctatgg ataagatgga ttagcagcaa gggtctggct
     4921 aatgacagaa taagtctaga aatggattat ttttgtcata gactagtccg acttagtggt
     4981 tatctaattc acgatcttct ttagcttgtt gttctaaaag aatttgtgcg aagtcaaccc
     5041 acttagtgta gttattttcg gataattgtt ctgaaatcat ctctaactct cgttgtggca
     5101 actgataaga gactccctta ctttctaagg ttaaatcttc ttccattagg gcggttaaac
     5161 taacatcaaa gatagcagca atatcactta atagaccaga ctttggagta tattttcctt
     5221 tttcccactc actgattgaa gaagagcttt ttcgtcctag gagattagca agttctaatt
     5281 gttccatgtt cttttttaat ctaaggtact ttaaattact tgcaaaaaaa gttttcttgg
     5341 cggacatatt tttctccttt tctttatata aaatatgata ccataaaaga ggttaaagca
     5401 aaaggtatcg taaaagatgg tctaaaaact ctctaaaaac ttgacttcat aaaaaccgaa
     5461 gtttataata aacttaacat gattaactaa tctcatttta gtgtggaggg aaaattgtga
     5521 ccaaatcaac tttaaaagag ttaagaacaa caaagaaaat gacacagcag gaattgtctc
     5581 acttaacagg catttctgtc agaacgattg ctaggtatga aaaagatact aaaaagctaa
     5641 ggcgagcaaa atatgagaag ctaaaaggta ttgcacaagc attagcggtt tccgttgatg
     5701 atattttttt aggaatagat tcggaattta tgaactaaga cgatcgtttt ctgtttgcta
     5761 ttagtaagac caattaacaa aaaagtgctt agttatattg ctaagcactt ttatttgtta
     5821 atttgatgct attggcacaa tcaattgaga ctgactgaga tcgatagtta aagcatagtt
     5881 gctgttgtca cggacagtat gttcaaaatc ggttgtgtat aagatgactc gaagggtatc
     5941 tcctttttct agatgataga tagttggttg caaaggaagt ctaacagtca tccatttatt
     6001 attaggaata gtttcaattg acgacaaatt attgcgattt tgtaaattca tgaaaccttt
     6061 cgaaataaca cggtaagaat cttctctaaa tggtagctct ttaaggggtt ctctagagaa
     6121 attttgccca ttgtcaatac ttgattgggt gagagcaata ggaagatcgc ccaagcgttt
     6181 tttcttacca tagtcaagga tttgtgcaga tagaagtcct ttattttcac ttgatttcac
     6241 ttttaactgt aggacaattt caccgttaat aggtaagtcc tcttccagta aaatatcaat
     6301 cagggcttga ttggccttgc ctttaaagag agctgctttg gacgcacgga aatctttacc
     6361 gtaagcttta aattcatctt caccatagtg attatcaatt aaaagcagtt cctgaccgag
     6421 gtcaagctct ttgatggttg aggttccaaa gactttacgc tcctgccagt tttgtgggca
     6481 ggtgttatcc tgccagatca tttcaggaag gctaaaatca ttagctaggc ctaagagttt
     6541 ttgacataaa agggcattca tgctttcgcg aaaatcaatg gattgccaat tatgcatgta
     6601 aacatgctca ccttgatgta aaaagaggtg cttgttgatt gtggaaggaa gcgcattgaa
     6661 aatctcatag acctgtcttg gtttgacatt ccagtcttgt aagccatgac tataaacgac
     6721 atcacacttt atttgatgag cattttttaa gtaattgcgg tcgtgccaaa attgattata
     6781 atcaccagat tgtcggtcta acgcctgaga ctgttggttt agcaattctt gataatgatc
     6841 attatggcga agataatcgc cagccaataa attacgcgag taggtgagct cagttaggac
     6901 atctagatcc tctccaggat agccaccagg actacaaact aagccatttt cgcggtagta
     6961 gttgtaccaa gaagaaatag ctgattctgc aataatcatg gctaatccgt ctacgcctgt
     7021 agtggctaaa ccagtagaca tagttcccaa gtaggacttt ccagtcgtac aaactagacc
     7081 atttgcccaa tcagctctca cttggtgggt ttttgagtgg ctggtgtagg ctgtggctct
     7141 tccatttagc caatcaataa cggctttaaa gctttcaatt tgagcatagt ctccactcgt
     7201 cataaagcca gtggaaccag cagttcccac acctgaaaca tagatattag caaacccacg
     7261 tgcgaggaaa taatcgttga ggctatagga gttgatgtag ctaaagcttt ctagattttg
     7321 acctatagga agtttgcaag gctgtgtctc aagaggaata aaatccctgt cttcgacagt
     7381 gatttgtctc ctttttttaa cgactaactc tttttccata cgataaagtt ttttgtcgtt
     7441 ggctacttca ttgatacctt gatggtatgg actaggtgtc atcaaagttg gtaatggttt
     7501 ttgagattga ggacgaatga tattaacctt aataagatca agttggccat cttgatcggt
     7561 atcaagaggt gcttcaacgt aaacaacttc tcgaatcagc tgtgacgtgt caaaggtagc
     7621 taatgattta ccgttgaaga aatggtaatc attgtccata gtaagcattc cttggctgac
     7681 caaatcatca atcagggtca tgccagactt acaacgggtc gctaataagt ggtggatatg
     7741 cttgataatt tctgtttcgt caaagtggat aggaaaggcc agtttatcaa ggaaagcatc
     7801 ggcctctgta aaatcaactc caggaataaa gccaaggact tgcagggcca gtaaatcaaa
     7861 aacctctttg gataactctt gctctgattg aaaaaagctc aatgcgtcca tgtcattttt
     7921 ggcgattaga tgacttaaag gataatcact atcagggtag tggaagaaga ttttacgtaa
     7981 aaagctttct aggttagctt tggcagtttt ttgcagatcc agatcaaagc ccaattcttt
     8041 taattcttca gccgctcttt ctagactagt tggaatatag gaaaattgat tatagcgcat
     8101 aggagtttcc tttctagtgg cttagaaaac gatattatct tttaatttat caagattggt
     8161 aatgataata tgttgtttat caatgcgaat gatgtttttt tctttcaact gttttagaat
     8221 acggctgacg ctgctagcgg tagaaatgcc acagaattta ccgagttcct cattagtaat
     8281 gacaaaatta attaaaattt gaccatttgg tagttcttcg ccaaataggg tcattaagtc
     8341 atatatttga gtagaaatgg caccgatacg gccgttagtt aacatacatt gcattttttt
     8401 catggatttt tgaagacgat tatggtaaaa gtccttcaca tatccttgta gttcaatatc
     8461 ttcgtttata tcttttaaaa atgctgagcg gcgaaccttg taaaaactag ctttctctga
     8521 ctcgatgcga acattataag gctctcccat atcttttgaa taaccagtgt tgagaacaga
     8581 agtaatttca agtcctgtaa catatctcaa attaaattcc attccatatt ttgaaagaac
     8641 actctgtttc acaatgccgt cttttaaaat ataagtgaag tcctcttcga gactttcaaa
     8701 agtcaagtac ttgtgataac ttttttcaat gataggaaag ttatgattat caatataccg
     8761 ttgcaaaatg tgatgattca cgcttttctc caaatattct tttaccaatg tataatttgt
     8821 atcttcaaga gaaattaaca tttgctaatg ccctttgata gtaacactta tattatacca
     8881 ttgaaatata ttatattctg ctttttttgt aaaatgttta tgaaaataga gaggagttca
     8941 ttttcaatgg aagaaaaatt gtttaacaaa cattttgtgg ccatcacagt aataaacttt
     9001 atagtctata tggtctacta tctttttacg gttattattg cctttgtggc gactagagag
     9061 ctaggagctc agactagcca ggcaggattg gcaacaggga tttatattct agggacttta
     9121 ttagcccgtt tgatttttgg aaagcagttg gaggtgtttg gtcgtcgttt ggttttaaga
     9181 ggaggtgcta ttttttacct cttaacgacc ttagcctact tttatatgcc aacgattagc
     9241 atgatgtact tggttcgttt cttgaacgga tttggttatg gtgtagtatc aactgcgaca
     9301 aataccattg taacagccta tattccagct cgtaaaagag gagaaggaat taatttttat
     9361 ggcttgtcaa ccagtctagc agcagctatt ggcccttttg taggaacctt tatgttggat
     9421 aatcttcata ttgattttcg catggttatt gtcttatgta gcgtcttgat tgggtgtgtg
     9481 gttgttggtg cttttgcttt tccagtcaaa aacatgtctt taaatgcgga gcaattggct
     9541 aaaaccaaat cgtggactgt ggatagtttc attgagaaaa aagccttgtt tatcactgca
     9601 atcgcctttt taatggggat tgcctatgct tcagttttag gatttcaaaa attatacacg
     9661 tctgaaattc atttgacaac agtaggcgct tatttctttg tggtctatgc cttaatcatt
     9721 accatcactc gtccagcaat gggacgttta atggatgcta aaggcgataa atgggtcttg
     9781 tacccaagtt atcttttttt agcaatggga ctcttcttgc taggtagtgt atcgtctgga
     9841 ggaagctatt tgctttcagg agccttgatt ggctttggtt atggcacttt tatgtcttgt
     9901 ggtcaagccg cctccataca aggagtggat gagcaccgct ttaatacggc tatgtctact
     9961 tatatgattg gtcttgattt aggtttaggt gctgggcctt acctcttggg cttgattaaa
    10021 gatttggcac ttggtagtgg tgtggcttcc tttaggcact tattctggtt agctgctgtg
    10081 attccactga tttgtacctt gctttactta ttaaaaacaa agacaagaca ggttttgtct
    10141 taaggacaag aaaaggagtt tagtcttata cttaaataca aacgtatctt aacagctgta
    10201 gatagttcct atgagccggg attagctgtg ggtaaagcca ttactatagc gctttgaaat
    10261 aattctagtt tgctattcgt acatgtcaat aaaaatgact cgcaaaagcc attcgtgaaa
    10321 gagaaaatgt ggccttattt ttagtaggta caattggatt aagcacgttt agatacatga
    10381 caattggatt aacatcagaa tataggcttt gtcatgttaa gattgatctg ttgatcgtga
    10441 gagctaataa aaaatgttat aatactatga aataaagtga taaagataag gccttttatg
    10501 gtccttttct ttaccgcttt tattattgtg tgaaaataac acttaatgat cattttattt
    10561 attattttat tacctttaca ttcatcaata agcctgttaa aatggtattt gttatatttt
    10621 attatttgga ggaaaccaga aaacatggaa atgacatgga ctgtgaaata catcacagaa
    10681 tttatcgcaa cggctttttt gattattttg gggaatggag ctgttgctaa cgtcgactta
    10741 aaaggaacaa aaggacataa ctcaggttgg ttagtgattg cttttggcta tggcttagga
    10801 gttatgatgc cagctttgat gtttggtaat gtatctggaa accatattaa tcctgctttc
    10861 acagttggac tagcagtatc aggcttgttc ccatgggctc acgtcttaca atacgttgtg
    10921 gcacaattac ttggagctat ctttggtcag ttggtagtgg taatggttta taaaccttac
    10981 tttatgaaaa ctgaaaatcc aaatcatgtt ttaggctcat tctcaacgat ttcatctctt
    11041 gataatggtc aaaaagacag ccataaagtc tcttatatca atggtttctt aaacgaattt
    11101 gtaggttcat ttgttctttt ctttggtgct ttggctttga ctaaaaacta ctttggggta
    11161 gagttagttg gtaaattgat tgaagcaggt tacgaccaaa caactgcagc aacacaaatt
    11221 tcaccatacg tgacaggctc attggcggtt gctcacattg gtattggttt cttagttatg
    11281 gtcttggtta cttcattagg tggaccaact gggccagcac taaacccagc gcgtgatttt
    11341 gggcctcgtt tgttacacca cttcttacca aaatcagttc ttggtcaagc caaaggtgat
    11401 tcaaaatggt ggtatgcatg ggttcctgta gttgcaccta tcttagcggc gatagtagcc
    11461 gtagcagcgt ttaaatacct ttatatcaga taaaaaaaga actaaagctt cttttagact
    11521 caagacaagc tcgcttaagg aacttgtctt ttttatatga gttaagcgaa atctaatgca
    11581 aacttgactg tcttgtatag cgtattttgt aaattttact agacttcttg taaaaaagcc
    11641 tctcctcaga taagatgact aatgcctcta aacggtgtta caaataaacg aggcatccct
    11701 aacttccttt atctggtgtt gaaactattt tgcacggact aaatcactga tagagtaaaa
    11761 aacgattttt catttttgtt gtaaaatatt aaaaacgtct ctggttagtg attcccagaa
    11821 acgttatctt atctatttgt ttgttgctta tgagaggtaa aggaggaacg aatcagcatt
    11881 ccaccagtga agaaaacgat agcgatgacc agaagtttca agctattaat atctaaattt
    11941 gttaagaaaa acacagctag taaaacgcca ataataccac cagaaacaat tccagcaaaa
    12001 ccatcccaac tgacacggtt tgctttgata aactggctac tagaagctgt cataatcata
    12061 gctgcatcta gcatcataac aggcatagca actgttgggc taagacccat taatgaaaag
    12121 aaaatcaatt ctggagcata attgcctaac cccatagtca tcaagactcc aacgataaaa
    12181 ttaaaaccaa ttccaacaaa aagccagatg ccatggaggc catgaatggt gtctgaaagg
    12241 tgttctcctg gatggtagat tattctaatg atcatgatta tagctgctat gattaaaaga
    12301 ctaccaagga ttcgttggac agttggagca tgccagtttt ttgtaatatg agtccccaaa
    12361 taagctccaa taaaggctgc tgcagccata gcaagtaaag ttagaacctc aacttttacg
    12421 acaaaaataa agcaaagaga ttgaatgagc accggaagca catgagctac ggtcatagtg
    12481 ccaggcaatt gtctatcgtc agtgactaat ttagttaact taaagcacgt tgtggtagta
    12541 gcaaaagacc ctatcccaag ggtgtcaagg gcatctgtta gcaggccaat ccaaaacccc
    12601 gtccaaaagc gtttaaaagg attgatctta ttttttttaa gatgttttac taacataaag
    12661 aaaataaaaa gcattgccga aactagcaaa gcttgtatca gtctaaggat aagcatttcg
    12721 ttcataaaga ctagtttaca aaaaaaaacc ttagccgtca acgtgattta cttcattaaa
    12781 acatgccact atgcttagaa gagctttggt tcgatgtatt ttgttaaaat taagctaaaa
    12841 tattaatatt gcatgaaagg ggacgccttc ctttttccct tactgagctt agctgttatt
    12901 gtagtgaatg tttaaaaggc tagtttgtcg ctctttatca aaaatctagt aggatgaata
    12961 cgtataattt cttgtcaaat agaggaattt tgtctataat agcagaggat taggaggaaa
    13021 ttatgacttt aatggaaaat attaccttta aaactccagt attacgcgtt aatgatcgcg
    13081 accttaatat tgcattttat caaaataatt tagggcttcg tttagtatct gaagaaaatg
    13141 cgattgctat cttttcatct tggggagaag ggcaggaatg ttttgtaatt gaagagtcac
    13201 cttcagttag aacgcgtgcg gtagaaggcc ctaaaaaggt taatacgatt gtcatcaaaa
    13261 caaatcaacc taaagaaatc gaacaattat tagctcatgg agcacattat gacgctcttt
    13321 ttaaaggtca aaatggctat gcctttgaga ctatttcccc agaaggagac cgattcttgt
    13381 tgcatgctga acaagatatt aaacatctcc aaggtactga tttaccatcg cttgaaaaag
    13441 atgcaacttt taaaggttta actcaattta agtttgatat tattgtttta aatgttatta
    13501 gtgaagaacg ctcaaaagct ttttatcggg atctgtttag tgatcagtta cctattacta
    13561 tggattttat tcaagaagaa ggtcccgatt tggcaattga tccccatatt gcttgggatt
    13621 tagaaatttt agaatttcaa gtctcaaagg actatgatat gaaggtccta aaagcaactt
    13681 tagaagaaga cgggcacaag gtctacattg ataaaaaaca caaagtattg gtcctttctg
    13741 atccaagtca gatcgaagta tggtttacga aatgactttg gcagtcatta aatgtattga
    13801 aaaccatctt cacaaaaaag tatacaaagg ggccagtctg gctctctttc aatctggtcg
    13861 atggcaagaa taccatatcg gaacaattga tggaagacga ccagttgatg ctaacttagt
    13921 ttatgacttg gctagtgttt caaaagttgt tggtgtggcc acaatatgca atattttatt
    13981 gaataatggt acgttagcat tagatgatcc cttaaaagca tattatccta gtattgttga
    14041 tgcgactgtc actattcgac agttattgac tcataccagt ggtcttgatc cttatattcc
    14101 taacagagat gttttaaatg cacaacaatt aagaaaagca cttaatcatc ttactcaaaa
    14161 aggaaataaa aacttttatt acacagatgt caatttcctc ttgttaggct ttatgttaga
    14221 agagctcttt agtgaatctt tagaccagat atttgacaag actatcttta ctccctttgg
    14281 gatgtatcat acgtcttttg gccctcgtcc agaagctgta ccaacattaa agggtgtgag
    14341 tgatggggaa gttcatgatc ccaaagcaaa aatcttaaaa aaacattctg gatctgcggg
    14401 gctattttca actcttgcag atttagaaag ttttagcaat cattatctta atgatccatt
    14461 ttcagattgt ctgtggcgca actacagcca acaaacaatt gagcggtcac tagggtggaa
    14521 tttagatggc gattggatta gtcatacagg ttacacaggg ccgtttctaa tgcttaataa
    14581 aaaagagcaa acggcagcta tttttttgac caaccgtact tacgacgagg atgataaaag
    14641 taaatggtta aaagaacgtc agttgcttta caatgcacta aagcatgatc tgacaacacc
    14701 cgtatcgtaa cgtctaaagg ggcagtttta gcgatcttat tactgttttt aaagtttctt
    14761 ctaaaaaata gccatttcat ttgtctgatg agattagagt gattataagt gaaaaaagaa
    14821 cagccatctc attttgaatc aaatgagatg gctgttcttt tttataagtt attgatcatt
    14881 tagatactac tatttacttg aaatagcttc agcggcatca tccattgaaa gcacttcgtg
    14941 gaagacacgt tgagtcaatt cagttttttg ttctggtgta aggtatttgg tgttgacaca
    15001 gtaaccagag atacgcacga taacgtcttc gccgttcatg attttgtcat aaacatcgtt
    15061 aaggtccata acgttcaagt taacgtgctg accaccattt tcaaagtaac cgtcaagcac
    15121 tgttaccaag ttgtcaactt gttcgtcaaa tgttttacca agagcacgag gtgatacttg
    15181 agtagtcaat gagataccat cgttagcatg tgagaattcc agtttagcaa gtgagttaag
    15241 gttttgtaac cagccacctt ttgctttgtt agatgggtta gcacctggtg agaagaattc
    15301 cacttggcta gtgttaactg taccatcttc gtttaagaag acgccgcggt gaactggaga
    15361 gttaccagtt tgtttagagt aagcaacgtt tgaagtgatg gtaagaagtg atactgaagc
    15421 ctcagcattc ttgtaaagtt tgtggcttgc aagacgtgtg tggtaagctt ccatcaacca
    15481 tttcgcgatg tcatcaacac ggtcgtcatc ttcaccgtaa cgagggaagt cacctgttac
    15541 ttcgtagtca tagatgtagc cattttcatc gcggatagtt ttaacagttg cgtatttaat
    15601 agctgacaag gtatcaactg tgttagcgaa accacagata ccgaatccca tgttagcacg
    15661 ttggtgtgtt ggcaagaagg ccatttgaac tgcttcgtag ttgtatttat cagtcatgta
    15721 gtggatgatg ttaagcgcat ctacataagt atctgtcaac cagtcaagag atttctcaaa
    15781 gttagccata acttcgtcgt actcaaggac ttctgatgtg attggttcaa caacgttaaa
    15841 cactttgtag tctctgtgaa catcatcgta accaccgtta agaccagtaa gaagggcttt
    15901 gagaacgttt acacgagcac cgaagtattg gatgttatga cgttgttctt cattttctgg
    15961 gtcaagtggt gatacacaac atgaaataca tgacatttca ccataacctt ctttagccat
    16021 tgttgtcaca ccttcatatt ggatagaaga gtgtttgtgg ctcattttca tacagtagcg
    16081 acggaatgtt tctggcagtt ggtcagtcca aagaactgtt aagtttggtt ctggagagtt
    16141 accgatatta tcaagagtgt ttaagaaacg gtagtccatc ttagtcacac ggtgacgacc
    16201 gtcgttaccc ataccagcca tagaagttgt gatgaatgtt gggtcacctg agtaaagggc
    16261 atcgtaagct ttagtacgac caaattttac ggtacggagt ttaaggacaa aatcatccac
    16321 aaattcttgg atttctgatt ctgtaaaggt accgcgagca aggtcacgtt ctgcaaagat
    16381 atcaagaacg attggcacac gtccaagaga agtagcagca ccgttgatca cacgacatac
    16441 agccatgaaa gcgatgttta cccattggat agcttcttta acgttttgtg ctgggcgacg
    16501 aacatcaact ccgtaaaggt caccaagttt aaccacttca ccaagagctt ggtattgaag
    16561 gttaacttct tcgcgaagac gaatggattc ttcatcgatt tcagtgatag cattccagtc
    16621 attgactttt tcttgcatca agtagtcagc accgtaaaga gcaagacgag cgtaaacacc
    16681 gatgatacgt ccacgtgagt aagcatctgg aagaccagtt acagtgtggg catgacgagc
    16741 acgacgaata tttgaagtat aagcgcggaa gataccgtcg ttaacagttg tcacgtactt
    16801 agtaaagatt tcgtgtactg ctgggtctgg ttcatagccg ttttctttaa gggtcgtttc
    16861 cgccatacgg ataccacctt ttggcatgaa gttcaatttg aacaattcat cgttttgaat
    16921 accataaatt aattcatttt ctttatcgat gaaaccagca ggaatgtcag caattgaagt
    16981 tggacgagta tcgtatggga aacgtgtagc ttcatagtgt gcttttgttt cttcgataac
    17041 ttttttgatg tgaagtgaac gttcagtagc accagccaag aaagattcat cgccatcgta
    17101 tggggtgtag ttagcttgta caaaacgtga gacacttgct ttttctttcc agtcagttcc
    17161 tttaaagcct tcccaagctt tttcgaaaac atctgtattg gttttaacag ttgccataaa
    17221 tttctcctta aattctagta acaaagcgat ctgttacatt tacaattcta agtttaacat
    17281 ataggaaacg ttttcccaag caaaattctt gacaaaatta agattctttt ataatacaga
    17341 ttgttgaaac agtcacaaac tgtattagaa tgtatttttt tggctttaga tagcctaaaa
    17401 ggtataatag agaaaggagg gacttatgct tatttttcca ctgattaatg acacgtcacg
    17461 aaaaatcatc catattgaca tggatgcctt ttttgctgca gttgaggaaa gggataaccc
    17521 tgctttaaaa ggaaagcctg ttgtgattgg gaaagatcca agagaaacag gtggtcgcgg
    17581 agttgtttcc acttgtaatt acgaagcgag aaaatatggc attcattcgg ccatgagctc
    17641 taaggaagct tatgagcgtt gtcccaaagc catttttatt tcaggaaatt atgaaaagta
    17701 tcgaacagtt ggggaccaga tccgccgtat ttttaagcgt tatactgatg tggtagagcc
    17761 catgtccatt gatgaggcct atctagatgt caccaacaat aagttgggta taaaatcagc
    17821 cgtcaaaata gccaagctga ttcagcatga tatctggaaa gaagtaggat tgacctgttc
    17881 agcaggtgtg tcttataaca aatttttggc taaattggct agtgattttg aaaaacccca
    17941 tggcctcacc ctagtcttga aagaagatgc cctgtgcttt ttagccaaac tccccattga
    18001 aaagtttcat ggtgttggta aaaaatcagt tgaaaaactg catgacatgg ggatttatac
    18061 aggacaggat ttgttggcag ttcctgaaat gaccttgatt gatcattttg gtcggtttgg
    18121 ttttgacctt taccgtaaag cgagaggcat cagcaattct cctgtcaagt ctgatcggat
    18181 acgcaagtca attggcagtg agagaaccta cgctaaactg ctttatcaag aaacagacat
    18241 caaggcagag atcagtaaaa atgctaagcg cgtggccgct ctcttacaag accataaaaa
    18301 gttaggcaag actattgtac tcaaagtgcg ttatgctgat tttaccacct tgacaaaacg
    18361 tgtcaccttg ccagaattaa ccagaaatgc cgcacaaatt gagcaagtag ctggggatat
    18421 ttttgacagc ttaagcgaaa atcctgctgg tatccgcctg ttagggataa ccatgaccaa
    18481 tttggaggat aaggtagctg atatttcctt ggacctatag gagaaagagt gctctatccg
    18541 tccgcctcat tttggagaaa aaagaagctg attagggatt tccctaatca gcttctttgg
    18601 tccattattt tattacagca tagcttggtc tgtatcatca cctttttcgt taagggtttt
    18661 agccttgtaa aaagcaaagc cataaagagc agcttgtata aggataagga tagttccaac
    18721 cattgacatg gttgttgtga actggttaac caagccgata acagaaagga taaggcctaa
    18781 gatataagga atataactaa ccgttagacc ttgttttaat ttagataagt tttgagcaca
    18841 aaagacaatg atagcaataa tagcaaggac atttaagata gataagaaaa tcataaatgg
    18901 tgtcatgctg ctttgtagct gtgccaattg ctcagttgtt aattgggccc gaaatgcttc
    18961 attcttgagt tggctttgta agtagaaaat ccctgcaaat cccattaatg acagtacaac
    19021 tccaagcccg tttagaataa taatggcaat agtagacgtt ttgagtgcct gacgcacttt
    19081 ttcataagaa atcatataat cctcctagag ttcttaatcc gaaattgtac aatgtgttta
    19141 atacaaaaat attataccat agattaatca aaaatgtccg tttttctagg attttttctg
    19201 gaaaaagagg gcaatatctg actctgtaag ccctatcatc gaatcgatag tcgaataatt
    19261 ctcaggtgtt aacctaaaat catcattatc tgactgagag ttctcagtgg ggttagagtt
    19321 tgttgattga gaggaggcta agtcggattg ttcttggaag cgttcgatca agtaggtttg
    19381 gcgtttatcg ccttcgtgtt taatagcata ctcaaaggca gtatattctc ccaacagaat
    19441 taacttactt ttagaccgag taatggccgt gtaaatcaca tttcgttgca agagtcggcc
    19501 gctttggcgt gtgataggta aaatcaccac ttgaaactca ctcccttgcg atttatgaat
    19561 gctcatggca taggccaggg ttaattttag ccattcattt ctagggtacg tgacttcgct
    19621 accgtcaaaa tctaagatta attcgtcttg cttagattcg gtgtatttag caggaatcaa
    19681 atctgtaata taaccaatat ctccgttaaa gacattcaac tgagcatcgt tgactaagtg
    19741 caggacttta tcacctttac gaaaatgcgt atcattgaac agaaattctg tttgtccttg
    19801 caagggattg agaagttctt gcatgagttg attaagatga ttgatgccag cttgtccttt
    19861 gtacataggc gctaaaattt gaatttcctc agcagggatg ccacttttaa tagcagatag
    19921 gacaattttt tgaatcatgt cagggataaa ggctgcttgg gcttcaaaat aagaacggtc
    19981 agctttttta tcacgaaagt cagcagctaa gattcctcga cgcatctggt ctgccaaatt
    20041 gacaatagta gattcttggg attgacgaaa tatcttttgc aaggcgattt ggggcaggct
    20101 attgactttt aaaagatctg acaggacttg accgggtcca accgaaggaa gctggtcact
    20161 atctccgaca ataatcactt gagttgttga attaatagct cctaacaact ggttggcaag
    20221 ccaggtatcc accattgaaa attcatcgac aatcagcagg tcgcagtcta gataatcctc
    20281 catggcttgg taatcgttgt cgccattgag gcctaagtgc ctgtggatgg ttgcgctagg
    20341 aagtccagtc aactcattca tgcgacgtgc agcccgacct gttggagctg ctaacaagat
    20401 aggaaggtct tttttatcca aatcaatctg atgcaggttc gcgtaagcct gcaaaatgcc
    20461 tcgaataaca gttgtttttc ctgttccggg accacctgtt aggagaaaga ccttgctggt
    20521 taaggcctta gtaatggctt cttgctgcac ttggtcatag gtgatggcaa agtctttttg
    20581 aacggcttgg atggttgttt gaatggtatc atgactaaat gactgactta aaggggtatc
    20641 taagagacga gagatgttgt tagcaatccc ttcttcagca aaataaaggc tagcatcaaa
    20701 cagtttggtg tcactgtttt tgattttgcc ctctatgatc aactcgctta gttgttcagc
    20761 cacagcagca ggatcacatt ctacttgacg tgcatcttca aggagtgtaa tggcaaagtc
    20821 taataattgt cgtgcttgaa cataagtatc tcctcgattg atggattctt ccaagagaca
    20881 gtgaaggagg gctgcacgaa aacgtttcgg agag
//
LOCUS       DAUVUY010000026        20798 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.026, whole genome shotgun sequence.
ACCESSION   DAUVUY010000026 DAUVUY010000000
VERSION     DAUVUY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20798
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.026"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..663
                     /locus_tag="VOX58_000838"
     CDS             <1..663
                     /locus_tag="VOX58_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HES5878084.1"
                     /translation="PFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALV
                     INLKSGQYYLIEKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQ
                     DDFTFQSKMKSTLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKDVIPEPIKIS
                     DIDHSRQIKKLENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIK
                     TK"
     gene            664..1263
                     /locus_tag="VOX58_000839"
     CDS             664..1263
                     /locus_tag="VOX58_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HES5878085.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            1273..2991
                     /locus_tag="VOX58_000840"
     CDS             1273..2991
                     /locus_tag="VOX58_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878086.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLVSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEVPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPINDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGE"
     gene            2994..4721
                     /locus_tag="VOX58_000841"
     CDS             2994..4721
                     /locus_tag="VOX58_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878087.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQLVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLIPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEVLRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRGEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            4962..6332
                     /locus_tag="VOX58_000842"
     CDS             4962..6332
                     /locus_tag="VOX58_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HES5878088.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDTKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDMSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(6491..7474)
                     /locus_tag="VOX58_000843"
     CDS             complement(6491..7474)
                     /locus_tag="VOX58_000843"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HES5878089.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            7665..10151
                     /gene="gyrA"
                     /locus_tag="VOX58_000844"
     CDS             7665..10151
                     /gene="gyrA"
                     /locus_tag="VOX58_000844"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HES5878090.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIAEINTDRENSISKNKDN"
     gene            10171..10920
                     /locus_tag="VOX58_000845"
     CDS             10171..10920
                     /locus_tag="VOX58_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HES5878091.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQVST"
     gene            11003..11416
                     /locus_tag="VOX58_000846"
     CDS             11003..11416
                     /locus_tag="VOX58_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HES5878092.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRSDKHDYK
                     LDLSCGRIELEIFGKVTSDPNYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
     gene            12030..12464
                     /locus_tag="VOX58_000847"
     CDS             12030..12464
                     /locus_tag="VOX58_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878093.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIATE
                     TVEESLGQVKEKVEGIGEATSQTIQSKMEKLTSDKTDADDEK"
     gene            complement(12514..13425)
                     /locus_tag="VOX58_000848"
     CDS             complement(12514..13425)
                     /locus_tag="VOX58_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HES5878094.1"
                     /translation="MKTVRIFYNPNSGKKENQLARQVKDYFCQHGFSEDSVEVITPKD
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIMQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGEFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(13542..14192)
                     /locus_tag="VOX58_000849"
     CDS             complement(13542..14192)
                     /locus_tag="VOX58_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HES5878095.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(14189..14629)
                     /locus_tag="VOX58_000850"
     CDS             complement(14189..14629)
                     /locus_tag="VOX58_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HES5878096.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLLQEVIQ"
     gene            14848..15696
                     /gene="ylqF"
                     /locus_tag="VOX58_000851"
     CDS             14848..15696
                     /gene="ylqF"
                     /locus_tag="VOX58_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HES5878097.1"
                     /translation="MATIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTHY
                     PSRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDVD"
     gene            15686..16477
                     /locus_tag="VOX58_000852"
     CDS             15686..16477
                     /locus_tag="VOX58_000852"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HES5878098.1"
                     /translation="MSTSIKAIKESLEAVTSLSDPLFQELATDARLGVQKALKSRQKA
                     IQADLAEEERLEAMLSYEKALYKEGYQAIAGIDEVGRGPLAGPVVAACVILPQHCKIK
                     GLNDSKKIPKSKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            16542..17378
                     /gene="dprA"
                     /locus_tag="VOX58_000853"
                     /pseudo
     CDS             16542..17378
                     /gene="dprA"
                     /locus_tag="VOX58_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
     gene            17485..19614
                     /gene="topA"
                     /locus_tag="VOX58_000854"
     CDS             17485..19614
                     /gene="topA"
                     /locus_tag="VOX58_000854"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HES5878099.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGSLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMAEGETVKKISTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(19689..20573)
                     /locus_tag="VOX58_000855"
                     /pseudo
     CDS             complement(19689..20573)
                     /locus_tag="VOX58_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            20586..20798
                     /locus_tag="VOX58_000856"
                     /pseudo
     CDS             20586..20798
                     /locus_tag="VOX58_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
BASE COUNT         6676 a         3628 c         4199 g         6295 t
ORIGIN      
        1 cccttttttg ctttcttgcg aattgctttg aaagaacagt ttgctcacct ttctgacaat
       61 tatgaacatc catttacggt gacgcagaat aatctgccga gtcccaccca gacaccagat
      121 gaagcactag tcattaatct caaatctggt cagtactact tgattgaaaa gcgtgtgaag
      181 cataatggca gttttgctaa ttatttttca gaacacctct tgaaagttac acctgagcaa
      241 tctgttaaaa agtctatcaa aatgatagaa caaaccgctc aaaaaattgc agagcatttt
      301 aatcaagatg attttacttt tcagtcaaag atgaaatcca ctctttttaa gcagttagaa
      361 gcagacgacg ttttatcacc agaaaaatta gctgaccaac tctttgatga taatttaaca
      421 gcccgtttaa cctttgtgga tcaagtcaaa gatgtcattc cagaacctat aaaaattagc
      481 gatattgatc attctagaca gataaaaaaa ctagaaaatc aaaagctatc gttatcaaac
      541 gggatagaat taacagtacc aaatgccatt tatcaggatg ctgaagcggt tgaatttttg
      601 ttaaatgatg atgggactta ttcaatcttg attaaaaata tagaggacat taaaaccaaa
      661 tagatgttta gacttttaaa gcgtgcctgc agttttcttc ttctttttgt catttatcaa
      721 tcctttgtta ttcatcacaa tgttcaacgt gttttggcat ataagcctat ggttgagaaa
      781 acattagcag aaaatgatac aaaggcaaat gtagacttag tcttagctat gatatacacc
      841 gagacaaaag gaggagaagc agatgtcatg caatccagtg aaagcagttc aggacaaaaa
      901 aactcgatta cagatagtca ggccagtatt gagcacgggg tgaatttact gtctcataat
      961 cttgctctag ctgaggaagc aggagtagat tcttggacgg ctgttcaggc ctataatttt
     1021 ggaacagctt atattgatta tattgctaaa catggaggtc aaaataccgt tgatttagcg
     1081 actacctatt ccaaaaccgt tgtggcgcca agcctaggca acactagtgg tcaaacctat
     1141 ttttattacc accccctcgc cttgatttct ggaggtaagc tttataagaa tggcggcaat
     1201 atctattact cacgagaagt tcatttcaat ctttatttaa ttgaactgat gagccttttt
     1261 tagaaagtta tcatgaaacg attacgtcca tatttgaaag ggtacctaaa agaaagtatc
     1321 ttaggtcctc tttttaaatt attagaagct ttatttgaat tattagtccc tttgttaatt
     1381 gctaatatga ttgatatatc gattagtcaa cacaacagcc agggaatttt gagggttgtt
     1441 ttaacattat ttggtttagc aaccattggc ttattgcttt ccgttacagc ccagtatttt
     1501 tcttcgaaag cagctgttgg ttttacaaga caaatgacag atgatttgtt taaaaaaatc
     1561 atgtttttga gcaaggagga ccaagaccat cttggttatg ctagtctggt atcacgattg
     1621 accagtgata gttttcaaat tcaaactggt atcaatcaat ttttgcgtct ttttctaagg
     1681 gcccctatta tcgtatgtgg tgctatggta atggcttatt ggattagtcc aagtctaacc
     1741 ctatggtttg tgatgatggt gattgtctta ctaacacttg tttttgttat gtcacattta
     1801 ttaggtcccc tttatctctt aatccgccga gaaacagatc acttggttcg tttgactagc
     1861 caacagttac aaggaatccg agtaattaag gcttttaatc aaacccaaaa agaactccaa
     1921 gcatttaagc agcaaaatat gctcttaagt cgccaccaat atcaagctgc taccctagcc
     1981 aatgtcttga atcctatgac ttttttagtg gtcaatctta ctctgttaat cttgatttgg
     2041 caaggaagtt ggcaagtagc tcatagatcg cttagtcagg gcatgttggt tgctttaatt
     2101 aattacctct tgcagatttt ggcagaactt ttgaaaatga ccatgttgat gggaaccatc
     2161 aatcaaagtg taacagccgc aaaacggatt aatcaggtgt ttgttttagc tgatgaagtg
     2221 cctttgccct tacttaaaga tggtcctatt tcaactcatc tgttgaccat ccggcacttg
     2281 acctttactt atccaggagc agcagagcca agtctgtatg atattcagtt aagtgctgat
     2341 cagggagaat ggattggcat cattggtggt actggagcag gcaaaacgac tttgattgac
     2401 ttgatctgcc aaacttattc acaatatagt ggtgagatta gtcttaattg gcaaggagaa
     2461 gtgccaaaaa cactaaccga gtggcgaaat gtgattgctc ttgttcctca aaaagctcaa
     2521 ttatttaaag gaacgattag aagtaacctc ctactgggac aatcaatgcc tattaatgat
     2581 gaggagctgt ggcgggctct tgaattagct caagcaaaag agtttgtcgc tgccttacca
     2641 gaacaattag aggcacctgt tgaagccttt ggccgtcatt tttcaggagg gcaacggcaa
     2701 cgtctagcta ttgccagagc acttctgaag ccaaaaccca ttttgatttt ggatgatgcc
     2761 agttccgcct tagacaatga gactcgagga cgacttttta aagctctaaa agaagagtta
     2821 tcagatgttt tagtgattct agtcacgcaa tccattaaga atttgcagtt tgctgataag
     2881 attttagtgt tggaacaggg ccatcaattg gattttgcca gtcatgacca gttaaaagta
     2941 agcaatgcac tttatcaaga aatgctagcg ctttacgtga agggggagta aaaatgatta
     3001 aaacagacca tcacctccta aagcgagtgc ttcaggattt gttaaaaaaa cctctacctg
     3061 tttgtattct cgtgatagct agttttgtac aagttggttt aagtgtttac ttgcctgttc
     3121 ttatcggtaa agccgttgat atgagtttgt cagtcaattc ttggcaaact ttaaaatggc
     3181 tgttggggca aatgttagtt gttattgtgg tcaacacatt gattcaatgg gtgatgcctc
     3241 ttgtctatag tcgtttgcta tatcaatata gccagcaatt gaaagataag cttttggaaa
     3301 aaattcatcg tcttcctttt gcatatctag atcgtcaaac cattggagat ttggttagtc
     3361 gagtgataac cgacactgag caactgatca atggtctaca attggttttt aaccagttta
     3421 tacttggact attgacgatt ctgtgtacca ttatagctat ggcacagatt gactggctca
     3481 tgttaatctt ggttttggtg ttaataccaa gctccctctt tttagctcgt tttattgctc
     3541 aaaagagttt tcattatgca caagcacaga ccaaaagcag aggaaattta gctcagttta
     3601 ctgaagaagt ccttcgtcaa gaaggtttgg ttcaactgtt taatgctcaa gagcagtcta
     3661 tctgtgatta tcatgtttta aataaaacct attgcgaggc ttcacaaaaa gccatttttt
     3721 acgcctcaac agtcaatcca gccactcgct ttattaatag tgtcatttat gctttactgg
     3781 caggtttagg ggctgttcgt atcatggctg gacttttttc agtaggtcag ctaaccactt
     3841 ttttaaatgt tgttgtccaa tataccaaac ctttcaacga tatttcatct gtcttggcag
     3901 agatacagag ttctttagct tgtgcgcaaa ggctttatga ccttttagat attgaaataa
     3961 aagagcaaga gcactttcta acatttaagg catcagcggt taagggacaa attgactttg
     4021 aagaagtaag tttttcttat caaaaagaca ggcccttact gaaggatatt aatttttctg
     4081 ttcctgcggg ttccaaggta gcgattgtag ggcctacagg tgcaggcaaa tcgactttaa
     4141 tcaatttact catgcgcttt tatgaacttg atgctggtag catcaagtta gataaagttc
     4201 ctattaagtg ttatgctaag gaagaactta ggtccattac tggcatggta ttgcaagaaa
     4261 cctggttgaa agatgcgact gttcatgagt tgattgctta cggcagtgaa gaggctagcc
     4321 gtggtgaagt agtggcagca gccaaagcag ctcatgcaca cttctttatt atgcaacttc
     4381 ctaagactta tgatacttac ttaagtgctt ctgatgatgc tttgtcccaa gggcagctcc
     4441 agttattagc tattgccaga atgtttttga aaaaaccaaa agtcttgatt ctagatgaag
     4501 ccacctcctc tattgatatt agaacagaag ctgttattca agaggcacta aaagaactca
     4561 tgagaggaag gaccagcttt atcattgccc atcgtttatc aacgattcaa tcagctgatt
     4621 tgattcttgt tatggatcaa ggtcgattgg ttgagtgggg aacacatgcc agcttaatgt
     4681 caaaaaacgg atgttatgct agattacaaa agatagaata aagttcgtct tttaacaaag
     4741 gtgaactttt taagtcttcc taaagttagt taattaacaa actttaaact gttatataaa
     4801 aaaataacag cgaaaaagcg gacgatcttt ttttataagt gagaaaaaac agttgactaa
     4861 agaagaaata actcaaaaaa gtgagcaata tatttggaag cgttttttga ctgtggtata
     4921 atagagccct atataatatt gtgaaatgag gaacaaaata tatgagtaaa atcgttgttg
     4981 ttggtgcaaa ccatgctggt acggcctgta tcaagactat gttaacaaac tacggtgatg
     5041 ctaatgagat tgttgtattt gaccaaaact caaatatttc atttttaggc tgtggtatgg
     5101 cactttggat tggtgagcaa attgctggac cagaaggact tttctattca gataaagaag
     5161 aattagagtc tttaggggct aaagtttaca tggaatcacc tgttcaatca atcgactacg
     5221 ataccaaaac agtgacagcg cttgttgatg gtaaaaacca cgtggaaact tacgacaagt
     5281 tgatttttgc aactggctca caacctatct taccaccgat taaaggtgcc gaaatcaaag
     5341 aaggatcact tgaatttgaa gcaactcttg aaaatcttca atttgtgaag ttataccaaa
     5401 actcagctga tgtgattgca aagcttgaaa acaaagacat taaacgtgta gccgtagttg
     5461 gtgctggtta cattggtgtt gagttagctg aagctttcca acgcaaaggc aaagaagtgg
     5521 ttctgattga tgtcgtggac acttgcttgg caggttatta cgaccgtgat ttgactgact
     5581 taatggctaa aaacatggaa gaacatggta ttcaactagc ctttggtgaa acagttaaag
     5641 aagtagctgg taatggtaag gttgaaaaga tcattactga caaaaatgag tacgatgtgg
     5701 atatggttat cctcgccgtt ggtttccgtc caaacacaac tcttggaaat ggtaagattg
     5761 atcttttccg taatggtgct ttccttgtca ataaacgcca agaaacttct attccaggtg
     5821 tttatgctat tggtgactgt gcaactatct acgataatgc tactcgcgat acaaactaca
     5881 ttgctttagc ttcaaatgct gtccgtacag gaattgtagc agcacataac gcttgtggta
     5941 cagaccttga aggtattggc gttcaaggtt caaacggtat ttccatttac ggcttgcaca
     6001 tggtatcaac tggtttgact cttgaaaaag ctaaacgact tggttttgat gctgcagtga
     6061 ctgagtatac tgataatcaa aaacctgaat ttatcgaaca cggtaacttc ccagtaacca
     6121 ttaagattgt ttacgataaa gactcacgtc gtatcttggg tgctcaaatg gcagcccgtg
     6181 aagatatgtc aatgggaatt cacatgtttt cacttgctat ccaagaagga gtgaccattg
     6241 aaaagttggc attaactgat attttcttct taccacattt caacaaacct tacaactaca
     6301 tcacaatggc agcacttggt gccaaagact agtatctgat aggaaaaagc cagagcagtt
     6361 cagcagcact ggcttttttg ctttttaatt gttgcaacct gaaaagttct aaacttatga
     6421 ttatcctctt atagcacgtt ggaaaaggaa tatcaggcaa aacaaaaaga gaccgaagtc
     6481 tcttttgtga ttagttttta gcagcagaag caaattcttc cttagcaaag gcttcatcaa
     6541 tgatagcttt caattgattt gcagaggctt gcattttttg caattcagca tcgttaagtg
     6601 gaatgtttac tggacgaaca attccatacg ctccaacgat agctggttga ccaatatagc
     6661 aatcttcaac accttcgtat tggccttctt ggaaaacaga aagcggaaga acagcatttt
     6721 catcatcaag aatagctttt gtaatacgag ctagggcaac tgcaataccg tagaaagtag
     6781 cacctttttt attgatgatt gagtaagcag catcacgtac agaaatgaaa agatcaacta
     6841 ggccttgctc atcaatgtca cgattagctt ggagccagtc gtaaagacct acaccagcaa
     6901 cgttagcgtg tgaccaaaca gcaaattcag agtcaccgtg ttctcccatg atgtaagcgt
     6961 gaaccgaacg cgcatcaaca ccgattttag cagccagagc ttgacggaaa cgggctgaat
     7021 ctagagatgt acctgaacca atgacacgct ctttagggaa acctgagaat ttccatgttg
     7081 agtaagtcaa aacgtcaact gggttagcag caacaagaaa gattcctttg aaaccagatg
     7141 caacgatttg tgtaacaacc tctttattga tacgaaggtt tttttcaacc aggtcaaggc
     7201 gtgtttcacc tggtttttga ggagcaccag ctgttaagac aacaagatca gcatcatgac
     7261 agtctgagta gtcagctgca tagatttttt taggtgatgt aaaagctaga gcgtggctta
     7321 aatcttctgc atctccttga gttttttctt taaaaatatc aataatacca agttcttgag
     7381 caatattttg tgttaccaat gcaaaagcgt atgatgaacc gacggcacca tcaccgacca
     7441 agattacttt tttgtgttgt ttagttgcag tcatttctaa acatctcctt aattttatta
     7501 gagcaaaagc tcatacggtc ttattttaac atattgataa ttgtttgtca ttgaattcag
     7561 aaaaataatg aaaatagttt actgataacg cttccaataa gaaaaaataa ggaatttatg
     7621 gtataatgaa atctagtatt gactagaaaa ggagcattcc ttaaatgcaa gatcgaaatt
     7681 taattgacgt caatctgaca agtgaaatga aaacgagttt cattgactat gccatgagtg
     7741 tcattgtggc aagagctttg ccagatgtgc gtgatggtct gaaacctgtg catcgtcgta
     7801 ttctctatgg gatgaatgaa ctcggtgtga cacctgataa accgcataaa aaatctgctc
     7861 gtattacggg tgatgtcatg ggtaaatatc acccgcatgg ggattcttct atttatgaag
     7921 ctatggttcg tatggctcag tggtggagtt atcgccatat gcttgttgat ggccatggca
     7981 attttggttc tatggatggt gatggtgctg ccgctcaacg ttatactgaa gcgcgcatga
     8041 gtaaaattgc gcttgaatta ttaagggata tcaataaaaa taccgttaat tttcaagata
     8101 actacgatgg aagcgaaaga gaaccagtag tattaccagc tcgctttcct aatttattag
     8161 tcaatggagc gacagggatt gccgttggga tggcaactaa cattccgcct cataatttgg
     8221 cagagtctat tgatgctgtc aagatggtaa tggaacatcc agactgtaca acaagagagt
     8281 tgatggaagt gatccctgga cctgactttc cgactggtgc ccttgttatg gggcgctcag
     8341 gtattcatag ggcttatgat actggtaaag gttctatcgt tttacgctct cgaacagaaa
     8401 ttgaaacaac tcaaacaggt cgggaacgta ttgtggttac tgaatttccc tatggcgtca
     8461 ataaaacgaa agtgcatgaa cacattgttc gtttagcaca agaaaaaaga ctagaaggta
     8521 ttacagcagt tcgtgacgaa tctagccgtg aaggagtgcg ttttgttatc gagattcgtc
     8581 gagaggcatc cgcgacagtt atcttaaata acctctttaa attaacaagc cttcagacga
     8641 atttcagttt taatatgttg gctattgaaa atggtgtgcc taaaatattg tcactcagac
     8701 aaatcattga taattacatt agtcatcaaa aagaagttat tatacgtcgc acccgatttg
     8761 ataaagataa agctgaagcc agagctcata ttttagaagg cttactgatt gcccttgatc
     8821 atttagacga agtgattgct attattcgta acagtgagac agatgtcatt gctcagacag
     8881 aattgatgtc acgtttcgac ttgtctgaac gccaaagcca agctattttg gatatgcgtt
     8941 tgcgtcgttt gactggtttg gaacgtgata agattcaatc agaatatgat gatttgttgg
     9001 ccttgattgc tgacttatct gatattttag ccaaaccaga acgtattatc actatcatca
     9061 aagaagaaat ggatgagatc aagcgtaaat acgctaaccc acgccgtaca gaattaatgg
     9121 tcggtgaagt cctttctcta gaagatgaag acctaattga agaagaagat gttttgatta
     9181 ccttgtcaaa taaaggttat atcaagcgtc ttgctcaaga tgaatttcgt gcccagaagc
     9241 gtggtggacg tggcgtccaa ggaactggag tcaacaatga cgactttgtc cgtgaattgg
     9301 tatcaacaag tacccatgat actttgctct tctttacgaa ttttgggcgt gtctatcggt
     9361 tgaaagctta cgaaattcca gaatatggtc gtacagcaaa aggtcttcca attgttaatc
     9421 tattgaaatt agaagatggt gaaaccattc aaactattat caacgcacgt aaggaagaaa
     9481 cagcaggtaa atccttcttc tttacaacga aacaaggcat tgtcaaacgt acagaagttt
     9541 cagaatttaa taatatccgt caaaacggtc tccgtgctct taagttgaaa gagggagatc
     9601 agctgattaa cgtccttttg acaagcggtc aggatgatat tatcattgga actcactccg
     9661 gatattctgt tcgttttaat gaagcaagca tccgcaacat gggccgatca gctacgggtg
     9721 ttcgcggggt taagcttcgt gaagatgatc gcgttgttgg cgcgtcacgt attcaagata
     9781 accaagaagt tcttgtaatt accgaaaatg gctttggtaa gagaacaagt gctacagact
     9841 atcctacaaa aggtaggggg ggaaaaggaa taaaaacagc taatattacc cctaaaaatg
     9901 gtcaattagc tggattggtt accgtagatg gtacagaaga tatcatggtg attacaaata
     9961 aaggtgtcat tattcgcaca aatgttgcaa atatttcgca aacaggtcgt gctactttgg
    10021 gtgttaaaat tatgaaacta gatgctgatg ctaagattgt aacatttacc ttggttcagc
    10081 ctgaagatag tagtatcgct gaaatcaata ctgatcgtga aaatagtatt tcaaaaaata
    10141 aagataatta atcataacaa ggagtaaaaa atggtaaaaa aacaaaagcg tcgaaaaata
    10201 aaatccatgt cttgggcaag aaaactacta atagctgtac ttttaattct aggtttagca
    10261 ttgcttttta acaaacctat ccgaaataca ttaattgctc gaaactctaa taaatatcaa
    10321 gtaacgaaag tttcgaaaaa acaaatcaaa aagaataaag aggctaagtc aacatttgat
    10381 ttccaagccg tagagccagt tagtacagag agtgtcttgc aagcacaaat ggcggctcag
    10441 caacttcctg ttataggggg cattgccata ccagagcttg gcattaattt accaattttt
    10501 aaaggtttag gaaatactga gcttatttat ggcgcaggaa cgatgaaaga agaacaagtt
    10561 atgggaggag aaaataatta ttctcttgcc agtcatcata tttttggaat tacaggttca
    10621 tctcaaatgc tcttttcgcc gcttgaaaga gcacaaaatg ggatgtccat ctatttaaca
    10681 gataaagaaa aaatttacga atacatcata aaagatgttt tcacggtagc tcctgaacgc
    10741 gttgatgtta tcgatgatac agctggtctc aaagaagtga ctttagtgac ttgtacagat
    10801 atcgaagcaa cagaacgtat tattgtcaaa ggagaactaa aaacagaata cgattttgat
    10861 aaagcgcccg ccgatgtatt gaaagctttt aatcattctt ataaccaagt atctacctag
    10921 atatatctta aatataatat ttaaaaagta ggaccataga gaaaatttat ctcatcctac
    10981 tttttttgaa aggagaaata tgatgaaact aaatgccatt catcacgttg ctattattgt
    11041 atctgattat catctgtcaa aagattttta tgtgaataaa ctcggatttg aaattatcag
    11101 agaaaattat cgttctgaca agcatgatta caaattagat ttatcttgtg gtcgtattga
    11161 attggagatt tttggtaagg tgacaagtga ccccaactat caagctcctc ctaaacgtgt
    11221 aagtcaacca gaatttaaaa gtgaggcctg tggtttgagg caccttgctt ttcgtgttac
    11281 caacattgag agttatgtag atgatctaaa gagcttagga attccagtgg agcctattag
    11341 gcatgatgat tacacaggtg agaaaatgac tttctttttt gatccggatg gtttgccttt
    11401 agaattacat gaatagactt attaggttaa cgactggatc gtgctattaa actcgttaaa
    11461 aatagttatt tgttgtatct tcttttgctt gattaatctc ttttgatcgt taaatgattt
    11521 ggttaacttt caaagtgaag agcaattttt ggagtaatct tgtcgaagtt ttttaataga
    11581 ataactatag gaaaagcaaa atatttgacg ataaactctt acagtgttat aatgacaata
    11641 taagtaaata actgtgttgt cactttgttg aaatgttttc gtattaatgt ttttttatct
    11701 attacgatag ataattttac ttcaattttt gattgaaaat actaaaataa gtgatgtggt
    11761 cactttgaca accatttatt ggtcctttta gataagacaa ttttgtggct cttcagttga
    11821 aaggatgtgt tgttgatgtt agaggaaaca atcatgttaa attaactcag gcttaaagac
    11881 caaaaaagga gtgaagtcta ttatgaaaaa aagaaatttt aaacttacag aaatgttaca
    11941 attggtttta ttagtggttc ctgctgtaaa acttgctaaa aaaatgttta acgatagtaa
    12001 aacaccgtct aaaagaaagt aggtctacaa tgactaaaaa aaatcatggc gttttttggg
    12061 ggttgttgct tggatcagca gcagctagca ttgcttactt aagtttatcg tcttccaaaa
    12121 aagatcaact cttgaaagat agtgctaaaa aaatagatga tctcaacgct tacttgcaag
    12181 ataaaagtaa gcaagttctg gatgcagtat cggaaaaagt acaagaatcc aaagatgctg
    12241 ttgaagttta tggtggtatt gccgcagaaa ccgttgaaga aaagtggcaa gataccaaag
    12301 atgctgttga agtttacggt ggtattgcca cagaaacagt tgaagaatct ttgggacagg
    12361 ttaaagaaaa ggtagaagga attggcgaag ctactagcca aactatccaa tctaaaatgg
    12421 aaaaactcac cagcgataag acagatgctg atgatgaaaa ataggtttta aaataccttt
    12481 aagataagac aaaaagtgcc attaggcact tttttaatct tctgttggaa caataatcga
    12541 aatggctttt ggaatgatat ctaattgaat gggcagatag tcacttttat ccccatcaat
    12601 cctggtacgt ggtaatatcg cttgtttttt agcttgtggt agtaagctaa gcgtattggc
    12661 ttgaaaatga ctgatttcct ctgctttgga aaactcaccc tgtttaaaat cattaatatg
    12721 ccataaaaat ttaaagaaag agactttttt catggtataa acttggaaat agccatcgtc
    12781 agcttgagca ccaggagaaa aagaaggaaa accagcaatg gtatttgtca tcgtgattaa
    12841 gagaaacttc gttttcaggt gaatacgatg gttgtggcta attaaagaga tggcatagct
    12901 gcgatttcgt ttcagtattc gaatactatc ttttaaaaat gctagaggac caaagcgtcg
    12961 tttttcctca gccgttacgt cggcagcaat atcagccagt aatcctaagg ttaaactgga
    13021 aatcatgtat tgctggttag ctttgcaaat atcaacttgc atgatttgtc cagtcaagat
    13081 agtatccaac gcctcagtaa tttgtaaagg gatatgcata gccttggcaa aattattgac
    13141 tgtccctgag ggcactaagc ctattaaaca atgagctcct ccttcataga tgccaccgat
    13201 gattttattt agtgtcccat cgccaccaag agggataacc aagtcaatct tgtccttagc
    13261 agcttgtttt gctaattgaa aagcctggtc agcatcttta ggggtaataa cttctacaga
    13321 atcttcggaa aaaccatgtt ggcaaaaata gtctttgact tgcctagcaa gttggttttc
    13381 tttctttcca gaattaggat tgtaaaaaat ccgaacggtt ttcatcaggt ctctcctttg
    13441 acagcttatt acttgctatc cattatacca cttctaagaa ttgatgtctt acatcaaatc
    13501 atcacattaa gcctctcaat cagtctgagc atctatttgt cttaaagcat aaagaaagtg
    13561 atagcgataa attggagggc agaggccaat aaaataaaga gatgccaaat catgtggaag
    13621 tagggtcttt ttttggcata aaataccgct ccaatggtat aagacaagcc accaaataac
    13681 atgagaagac caaaagcaag actagttttt tgaatgatga ctggcaagat aaacacaacg
    13741 agccagccca taacgatata aagcatcaaa ctgaattttt cattgatacg tttggcaaaa
    13801 attttgtata aaatcccaaa aagcgtaatg ccccactgca aaacaataat gatatagcct
    13861 agccaaccag aaacaaggga taaggcaaca ggtgtataac ttcctgcgat agcaatgtaa
    13921 atcatactgt gatcaataat gcgtagaatg tatttgtgaa ccgaaccata agccatggag
    13981 tggtaaatcg tagaggacaa aaacatcaaa aagagactga tgacaaagat ggagatacca
    14041 atagcggctt taaggtcata agtttgataa gcatagctgg ctgaaatagg aagtagaatg
    14101 agcatagcaa atgctccgat agcgtgtgtc acgctgtttg ctacttcctc actaaaagat
    14161 aagggtaaac tttgtttaaa ggtttggttc attggatcac ctcctgtaat aaataaatag
    14221 ttttgtggta atctttgacc gtttgacagg caacctgcac aatcggtgga atgtcctcct
    14281 caatgggagc cttctgattc caacaggtta caaaagtatt gtaaagactt tcagaatcag
    14341 cctgtgcctg aagttcctcc aatacacgac taatatcttg aataggaaat accgttttaa
    14401 acaaactgat ggcaattaag cgcgctagtt gttgcttttg gtattttttc ttgataggtt
    14461 tagtcacata cccatgcttg acgtaattat taatcataga tgccgttagt gatttattat
    14521 ctgacacttc tgaaaaatca gtgcactggt tgacataaag taagacttga tccaaatata
    14581 agtctaaatc agggaggtcc ttccaataag gtaatgatgg aaaaatcata gttttgtctc
    14641 tctatctagt tttgataact agattatacg attaatgaaa acttattgca agcaaaaact
    14701 gtaaagactt aaaagaggaa agtagaaatg gcgaagtgaa gctaatcatg ctataatgtt
    14761 aaagaattga ttgggataga tgccttctgt cgcaatcaga ctattttatc acgaagaaga
    14821 tgatgctacg aaaggaattt aaactctatg gctactattc aatggtttcc tgggcacatg
    14881 tccaaagcca gaagacaggt tcaggaaaat gtaaagcatg ttgactttgt aactatttta
    14941 gtggatgcac gcttaccact ttctagtcaa aatcccatgc ttacaaaaat tgttggtgat
    15001 aaacctaaat taatgatttt aaataaagct gacttggcag atgcaactcg aactaaagag
    15061 tggaaggctt attatgaaag tcaaggtatt aaaacattag ccatcaattc caaagaacaa
    15121 tcaacggtta aaaaagtaac agaagcagcc aaggaactca tggctgataa aattcaacgc
    15181 ttgcgagagc ggggcattca aaaagaaacc ttgcgaacca tgattattgg cattccaaat
    15241 gctggtaaat ctaccttaat gaatcgcttg gctggtaaga aaattgctgt agttggcaat
    15301 aaacctggtg tgactaaagg acaacaatgg ttgaagtcca ataaggaatt ggaaattcta
    15361 gacactcctg gtattttatg gccaaaattt gaggacgaat tggtgggctt aaaattagct
    15421 ttaacaggtg ctatcaagga ccaattacta ccaatggatg aggtcactat ttttggtttg
    15481 aattacttta aaacacatta tcctagtcgc cttactaagc gttttaagaa tattcctctt
    15541 gaggaagaag ctcctgaaat cattatgacc ttgacacgcc aactgggatt taaagatgat
    15601 tacgaccgtt tctacacttt attcgtcaag gaagttcgtg atggtaaatt aggtcaatac
    15661 accttagatc aggtgggaga tatggatgtc gactagtatt aaagctatta aagaaagcct
    15721 ggaagctgtt actagcctct cagaccccct ctttcaagaa ttggcaactg acgctaggtt
    15781 aggtgtccaa aaagcgctaa aaagtcgaca aaaggctatt caggccgatt tagcagagga
    15841 agaacgatta gaagccatgc tttcttatga aaaagcgctt tataaagaag gctaccaagc
    15901 cattgcaggt attgatgagg tgggacgtgg ccccctagca ggtcccgttg tcgcagcatg
    15961 tgtcatctta cctcaacatt gcaaaattaa aggtctgaat gattctaaaa aaatccctaa
    16021 atctaagcat gagaccattt atcaggcagt gaaagaaaag gctttggcta tcggtatcgg
    16081 tattattgac aatcagctta ttgatgaggt caatatttat gaagcaacca aactagctat
    16141 gctagaagcc attaaacagt tggagggcca actcacacaa ccagattatc tcttgattga
    16201 tgccatgaca ttggatattg ctatttcgca gcagtctatt cttaaaggcg atgccaattc
    16261 cttgtctatt gcagcagcat caattgtagc taaggtcacc agagatcaga tgatggctaa
    16321 ctatgatcgc atttttcctg gttatggctt cgctaaaaat gcaggctatg gcaccaaaga
    16381 acatttacag ggattaaaag cttacggcat aacgcctatc catcgtaaaa gttttgaacc
    16441 tgttaaatcc atgtgctgcg attcaactaa tccttaaaaa gttctgtttt tagcagaatt
    16501 tttttctttt ttacgaataa atgaagtaag gaggtaactt agtgaatcat tttgaacttt
    16561 ataagctaaa gaaagctgga ctgactaata aaaacattct caatattctt gactaccaag
    16621 aacaccagga aaaatcgttg tcacttcgag atatggccgt tgtttctggt tgtaagcatc
    16681 cgtctcactt tatagaagcc tataagcagt tagatattca aaaattaaaa atagaattta
    16741 aacaatttcc tagtatttct atcttagata agcattaccc aatggcattg aaagaaatat
    16801 acaatccacc tgtcctcttg ttttttcagg gaaatttaga ccttcttgag aaacctaaat
    16861 tagccattgt cggctccaga cgctcaagcg ataccggagt aaagtctgtc cgtaaaattc
    16921 ttaaagaact cgggaaccgt tttgtgattg ttagcggact tgctcgaggt atcgacacta
    16981 gtgctcattt agcctgcctt aaaaatgggg gacaaactat cgctattatt ggaacaggat
    17041 tggatcgctt ttaccctaaa gaaaataggg agttgcaaac tttcttaggg aaaaatcatc
    17101 ttgtgctaac agaatacggt ccaggagaag aagctttatc ttatcacttt ccagaacgga
    17161 atcgtattat cgcaggcctt agccgaggta ttcttgtcgt tgaagcaaaa aatcgttcag
    17221 gttccttgat tacttgtcaa attgggatag aagaaggctg agacattttt gctgtcccag
    17281 gaaacatttt ggacgggaaa tccgaaggtt gccttcagtt aattaaagag ggagcaacat
    17341 gcgtcacatc gggcatggat atcctttcag agtactaaaa aagttaaagt ttcttcctat
    17401 atagcatttt tttattgaca gtttcttaaa aatgggatat tatgctataa gctttaaaca
    17461 aactgttata ggaagtgtgt cgaattggga acaaaaacta caactaagcc aaaaacgggt
    17521 actaaaaagt ccaccactaa aaagaaatca acagcgaaaa aaaatctcgt tattgttgaa
    17581 tcacctgcca aagctaagac tattgaaaaa tatcttggac gcagttataa agttgttgct
    17641 tcggtaggac atattcgtga cttaaaaaaa tcgtctatgt ctattgattt tgataataat
    17701 tatgagccac aatacattaa tatccgtggt aagggatccc ttatcaattc tttaaaaaaa
    17761 gaagccaaag ctgctaaaaa agtttattta gctagtgacc cggatcgtga gggagaggct
    17821 atttcttggc atttgtcaca tatcttagga cttgatcctc aagataataa ccgagtggtt
    17881 tttaatgaaa ttacgaaaga tgcagttaaa catgcctttg tggagcctcg tcagattgat
    17941 atggacttgg tagattctca acaagcacgt cgtgttttgg accgtattgt gggatattcc
    18001 atttcaccaa ttctttggaa aaaggtcaaa aaaggacttt cagcaggacg tgtccagtcg
    18061 gttgctttaa aactcatcat tgaccgtgaa aatgacatca aagcatttgt tccaaaggaa
    18121 tactggtcta ttgatggtct ttttaaaaaa ggaactaaaa agtttcaagc gactttttac
    18181 ggtattaatg gcaaaaaaac aaagcttgat aataacaacg atgtcaaaga ggtattagca
    18241 aagctaacaa atgaagattt tcttgtcagc aaggttgaca agaaagagcg acgtcgtaat
    18301 gctcccttac cttacacaac atcatctctt caacaagatg ctgctaataa aatcaatttc
    18361 agaacgcgta aaaccatgat ggtagcccaa caactgtatg aggggattca tttaggagaa
    18421 aatggaactc aaggtttaat tacctatatg cgtacggatt ctactcgtat cagtccagta
    18481 gcacaaaacg atgctgctca atttattatc aatcgctttg gagctaatta ttcaaaacat
    18541 ggtaatcgtg tcaaaaatac cagtggtgct caagatgctc acgaggcgat tcgtccatct
    18601 agtgttaatc atacgccaga ctctattgcc aaatacctta acaaggatca gttaaaactt
    18661 tatacgctta tttggaatcg ttttgtggct agccaaatga cagcagctgt ttttgatacc
    18721 gtaaaggtga atttggagca aaatggcgtg atttttgttg ccaatggtag ccaaatgaag
    18781 tttgatggtt acatggctgt ttacaatgat tccgataaaa acaaaatgtt acctgagatg
    18841 gcagaaggag aaacggttaa aaaaatatcg acctctccag aacagcactt tacgcagcca
    18901 ccagctcgtt attctgaagc aactctgatt aaaacgctag aagaaaatgg tgttggacgt
    18961 ccatcaactt atgccccaac cttagaagtg attcaacgca ggtactatgt taaattatct
    19021 gctaagcgct ttgagccaac tgagcttggg gaaattgtca acaagcttat tgtcgaattt
    19081 ttcccagata ttgttgatgt tgcctttact gccgagatgg aaggtaagct agatcaagtt
    19141 gaaatcggag aagaacaatg gcaacatgtc atcgatcaat tctatcaacc ctttgtcaaa
    19201 gaattgaata aggctgagtc tgaaattgaa aagattcaaa tcaaagatga gccagctggt
    19261 tttgactgtg atgtttgtgg ccacccaatg gtcattaagt taggacgttt tgggaaattt
    19321 tacgcgtgca gcaacttccc tgagtgtcgt aatacaaaag ccatcactaa agaaatcggt
    19381 gttacttgtc cggtttgtca taaaggacaa gttatcgagc gtaaaaccaa aaaaaatagg
    19441 atattttatg gttgtgatca gtatccagat tgtgagttta tctcgtggga tttacctatt
    19501 ggacgtgctt gccctaagtc aggtgactat cttattgaga aaaaagttcg tggtggcaaa
    19561 caagtaatgt gtagcaatga gacctgtgat tacaaggaag aaaaaattaa ataaaaaaag
    19621 aattggaact ccttgtcttg ttccaattct ttttttattt acaaaggtat ttttaactac
    19681 aaaaatcgct agttcgtttt taaaaggctt aaaaattcct caataactgg actgaaactc
    19741 gcatcatctc ggtaagcaat cccaataggg taaaaattat ttttatcttg caaaggaatc
    19801 caaactaagt tatctacctg attaagactc tcaaaatcac tcgggaggat agtcaaggta
    19861 tttaaatcgt gcaaactatg aataagaact tcccaatcat tgtgtttaaa gacaatatct
    19921 ggttcataac cgagagctcg gcattttcta ggaagcattt cgcctaacat gtagtgatcg
    19981 tttaaagaag caatcttata gccttttaaa tctttaagtt ttaactgagg atgttgcgcc
    20041 aaagggtgct gtttgaggag aacgatactg accttatagc cttttgtgga cgtttgtaat
    20101 agctcaatgg taatatcctt ttgaatactt agaaaggaga ggagaccaat atcgattttt
    20161 ccttgggaca ataattctta taatttgcga gaaccatcct gtataagcga taattcaaca
    20221 tttgagtgag tagccataaa gattgaaatt tgtttcatga attgtatagc aaacagtgat
    20281 attaattcaa cacgaatcat ctcttttttt cttgatttaa ttgctgaata tccgcaacaa
    20341 tttgattaaa gtcatccaca aggtctttgc ctttgtgata aagaatatct ccagcttcgg
    20401 ttagtagtag ctgtcaatca ctttgtgtaa aaagcgtcgt gttcaaacta ggactctagc
    20461 cttttaattg attgagaaag agtatgttgc gttacaaaca aacttttagc agcgtgggaa
    20521 tgattttttg tttcagcaac tgcaataaaa taggttaatt atctaatatc catagtagat
    20581 ccttcatgat tggtcaggga attgcgtctc tacttggttt acaacctatt ttgatgtctc
    20641 tccttattgc catgattttc tgcttcctta ttgtttctcc aattacaacg gtaggaattg
    20701 ctcttgcgat taacttatct ggtattggtt caggtgctgc aaacttaggt atttgtgccg
    20761 caagctttgg actttgttta gcaggttggg ctgttaat
//
LOCUS       DAUVUY010000027        20361 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.027, whole genome shotgun sequence.
ACCESSION   DAUVUY010000027 DAUVUY010000000
VERSION     DAUVUY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20361
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.027"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            57..854
                     /locus_tag="VOX58_000857"
     CDS             57..854
                     /locus_tag="VOX58_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HES5878100.1"
                     /translation="MTSKITIKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHI
                     SNLLNYTPNQAARALVTQQSNSVVLYYKYADSILARAEQLHTISFLSRLLHEQDYQLV
                     CLNEDSIEKFDWADLILTLDVDNDTFYKIGKLNFKPLIAIDSFVNDPLFFQINTDYAA
                     LKDQANQFFKDEPYTLLSLPITNRERYEHVSAFFPSLHIVNDLRELTYFEKENLLLID
                     ESLDQFFVNSSQKLYCPRFSPKKGQVILEAIEWAKKRQPDKKHNLLV"
     gene            complement(970..1785)
                     /locus_tag="VOX58_000858"
     CDS             complement(970..1785)
                     /locus_tag="VOX58_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HES5878101.1"
                     /translation="MPDKKQFPISYISHCLSARAMFASRAQFTWWQNLLIIVFLNALI
                     MIPVTLHYANMTTYPLERIVTKSLSPITDKTYQALTQGKIEKDTFQGQSLIRRDGELV
                     LAVLPTKVDLEQLASESTRQIIVTKKEWRFVTPDGKELRAHVRGQQQSLADLTTVKAV
                     KDFVNQQWYDSNKASVLGFLLLTFVLMVCVGTLIVIGLGAFFLTLTKRSRLFMIRNFS
                     EGLGLMVNCLAWPSLLAIALSFFIQDPVLIMNCQVFGTLLMLTWVFYKTQFRD"
     gene            complement(1807..2667)
                     /locus_tag="VOX58_000859"
     CDS             complement(1807..2667)
                     /locus_tag="VOX58_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HES5878102.1"
                     /translation="MRKTYVSISMKRKRFFTQLLTYLYLISLVIVILFPILVTVSSAF
                     RPGNTTAFSFHFDGPWTLSNFKTLFQDTLYLRWYWNTLIVAFFTMLIQVTVITLTGYA
                     YSRYNFFGRKKSLIFFLVVQMVPTMAALTAYFVMAWLFNALNQYWFLILIYVGGGIPM
                     NAWLMKGYFDTVPYDLDESAKLDGSGHFRTFYQIVLPLVRPMIAVQSLWAFMGPFGDF
                     MLAKFLLRAQENYTVAVGLQSFITNDARNPKVTLFAAGAILIAVPISVLFFFLQKNFV
                     SGLTSGGTKG"
     gene            complement(2664..3971)
                     /locus_tag="VOX58_000860"
     CDS             complement(2664..3971)
                     /locus_tag="VOX58_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HES5878103.1"
                     /translation="MVDNQRQQKPNKAMWLSVIPGLGQLYNKQIVKGGVLLVVFFLEL
                     LEIVVLGIPALTGFYSLGSVPMQDHSLFMLIKGAMQLITLVLFGIFHLVAMRDAKLVA
                     HQMNEGKKVPVTAKETLEAIYEKGFPYLLIIPAYLAMAFAIIFPVLVTLLIAFTNYDF
                     RHIPPYRLLDWVGLKNFLNILELSTFKTAFTSVLSWTLIWTLAATSLQIVIGIATAVI
                     ANQSFIRGKRLFGVIFLLPWAVPAFISIMSFGNFFNDSIGAMNVQVLPFIEKFLPFVD
                     FGIVSWKTDPFWTKVAVIMVQGWLGFPYIYILVSGILQAIPADLYEAATVDGATVLQK
                     FRHITMPMIFAVAAPTFISQYTFNFNNFSIIYLFNNGGPGTVGGGAGTTDILISWIYK
                     LTTQTSPQFSMASAVTLIISVIVISVSLITFKKFKAFDMEERS"
     gene            complement(4046..6181)
                     /locus_tag="VOX58_000861"
     CDS             complement(4046..6181)
                     /locus_tag="VOX58_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrin glucanotransferase"
                     /protein_id="HES5878104.1"
                     /translation="MRELHIKTYKLLTKSAVLLGLISFPLTVSAADNASVTNKADFST
                     DTIYQIVTDRFNDGNTSNNGKTDVFDKNDLKKYHGGDWQGIIAKIKDGYLTDMGISAI
                     WISSPVENIDSIDPSNGSAAYHGYWAKDFFKTNQHFGTEADFQQLVKVAHQHHIKVVI
                     DFAPNHTSTAEKEGTTFKEDGALYKNGKLVGKFSDDKDKIFNHESWTDFSTYENSIYH
                     SMYGLADLNNINPKVDQYMKEAIDKWLDLGVDGIRVDAVKHMSQGWQKNWLSHIYEKH
                     NVFVFGEWFSGHTDDDYDMTTFANNSGMGLLDFRFANAIRQLYTGFSTFTMRDFYKVL
                     ENRDQVTNEVTDQVTFIDNHDMERFATKVANNQTAVNQAYALLLTSRGVPNIYYGTEQ
                     YATGDKDPNNRGDMPSFNKESQAYKVISKLAPLRKQNQALAYGTTEQRWISDHVLVFE
                     RKFGNHVALVAINRDQTNGYTITNAKTALPQNSYKDKLEGLLGGQELIVGADGTISSF
                     ELGAGQVAVWTYEGEDKTPQLGDVDASVGIAGNKITISGQGFGNSKGQVTFGEISAEI
                     LSWSDTLITLKVPTVPANYYNISVTTADKQTSNSYQAFEVLTDKQIPVRLLINDFKTV
                     PGEQLYLMGDVFEMGANDAKNAVGPLFNNTQTIAKYPNWFFDTHLPINKEIAVKLVKK
                     DSIGNVLWTSPETYSIKTGHEAQTITIKK"
     gene            complement(6207..7910)
                     /locus_tag="VOX58_000862"
     CDS             complement(6207..7910)
                     /locus_tag="VOX58_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HES5878105.1"
                     /translation="MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGP
                     KYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTT
                     SYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGW
                     LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQF
                     GTKYDLQELIDLAHQMGIKIILDAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEH
                     PSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHD
                     FWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTS
                     KQLAEKLSNILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGI
                     PCLYYGTEVAMEGGYDPDSRRGFPWDDAERDKDFFSKVKAIIALRQQPALQEGAISID
                     YDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQDGYIVFS
                     N"
     gene            complement(8076..9335)
                     /locus_tag="VOX58_000863"
     CDS             complement(8076..9335)
                     /locus_tag="VOX58_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HES5878106.1"
                     /translation="MSWNWKKASVLGTLSLASVLPLTACVSGGGKGVKETDGKTIVVS
                     VDEGYVDYIKSIKGEFEKEHKVTVKVKKEGMMDTLDKLSTDGPTGASPDVFLAPFDRV
                     GGLGTEGQISEVTLGNSKEFDDTVKKLVTIDGKTYGAPDVIETLVTYYNKDLVPQAPK
                     SFTELEVLQKDSKFAFASEPGKSVGFLAKWTDFYYGYGLIAGYGGYIFGDKGTKPSDL
                     GLGNDGTVEGLNYAKQWYGTWPQGMQDTKKAGDFITEQFISKKAGVIIDGPWAASSFK
                     DAGVNFGVMEIPTLTNGKKYQPFAGGKAWVISNYSKGKTTAQKFLDYVTNAENQKRFY
                     DKTQEIPANLTARNYASKEGNELTKAVISQFESAQPMPNIPEMAEVWEPGANMFFNVA
                     SGKEEASKAAKEAAKTIKEAIEQKYAE"
     gene            complement(9944..10927)
                     /locus_tag="VOX58_000864"
     CDS             complement(9944..10927)
                     /locus_tag="VOX58_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HES5878107.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGIQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTALQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDCEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEPT"
     gene            complement(10958..12208)
                     /gene="dltD"
                     /locus_tag="VOX58_000865"
     CDS             complement(10958..12208)
                     /gene="dltD"
                     /locus_tag="VOX58_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HES5878108.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(12201..12440)
                     /gene="dltC"
                     /locus_tag="VOX58_000866"
     CDS             complement(12201..12440)
                     /gene="dltC"
                     /locus_tag="VOX58_000866"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HES5878109.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(12458..13714)
                     /gene="dltB"
                     /locus_tag="VOX58_000867"
     CDS             complement(12458..13714)
                     /gene="dltB"
                     /locus_tag="VOX58_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HES5878110.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(13711..15249)
                     /gene="dltA"
                     /locus_tag="VOX58_000868"
     CDS             complement(13711..15249)
                     /gene="dltA"
                     /locus_tag="VOX58_000868"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HES5878111.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGEHRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELASGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(15261..15404)
                     /locus_tag="VOX58_000869"
     CDS             complement(15261..15404)
                     /locus_tag="VOX58_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HES5878112.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
     gene            complement(15668..17659)
                     /gene="uvrB"
                     /locus_tag="VOX58_000870"
     CDS             complement(15668..17659)
                     /gene="uvrB"
                     /locus_tag="VOX58_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HES5878113.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIIYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            17851..20025
                     /locus_tag="VOX58_000871"
     CDS             17851..20025
                     /locus_tag="VOX58_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HES5878114.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDIVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            20025..>20361
                     /locus_tag="VOX58_000872"
     CDS             20025..>20361
                     /locus_tag="VOX58_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HES5878115.1"
                     /translation="MTELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVVVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKE"
BASE COUNT         6495 a         4123 c         3480 g         6263 t
ORIGIN      
        1 gccaaatgta ttaatgattc cttgactttt accatgaatt cggatataac aaaatgatga
       61 catctaaaat taccattaaa gatattgcac gagaagccgg ggtttctgtg gcgaccgtat
      121 cttacgtgtt aaaccagcgc aatgatcaaa aaatcagcga agaaacccgc aataaagtgc
      181 tacatattag taaccttctt aactatacac ctaatcaggc agccagggca ttagtcacac
      241 aacaatcaaa cagtgttgtc ctgtattaca aatatgcaga tagcatttta gctagggctg
      301 aacaactgca taccattagc tttctatccc gcctattgca tgagcaggac tatcagctgg
      361 tttgcctaaa tgaagatagc attgaaaaat ttgactgggc agaccttatt ctgaccttgg
      421 acgttgataa tgataccttt tacaaaattg gaaagcttaa tttcaaaccc ttaattgcca
      481 ttgatagctt tgttaatgat cccctcttct ttcaaattaa tacagattat gctgctctca
      541 aagatcaggc taatcagttt tttaaggatg aaccttatac cctgctttcc ttgcctatca
      601 ctaatcgtga gcgatatgag catgtttctg ccttctttcc aagtttgcac attgtaaacg
      661 acctaagaga gctaacctac tttgaaaagg aaaatctttt actcattgat gagagtttgg
      721 atcaattctt tgtgaactct tcgcaaaaac tctactgccc acgtttttcc ccaaaaaaag
      781 ggcaagttat cttagaggcc atcgaatggg ccaaaaaacg ccagcctgat aaaaaacata
      841 accttctggt gtaacgcctg agaattaacc tatcttgact aattccttgt aactcaaaac
      901 cttgcctctt tagataaaga agcaaggttt tttgatgtct aatctaataa cagggcctga
      961 cttgtctcat caatctctaa actgtgtttt gtagaacacc caggtcagca ttaataaggt
     1021 accaaatact tggcaattca taatcagtac tggatcctga ataaagaaac taagagcaat
     1081 cgccagtaga ctcggccaag ccaaacaatt aaccatcaat ccaagtcctt cagagaagtt
     1141 tcgaatcata aatagccgtg atcgtttagt cagagtcaaa aagaaagcgc ctagaccaat
     1201 cacaataagg gtacccacac acaccatgag aacaaacgtc agcaaaagaa aaccaagtac
     1261 actggcttta ttactgtcat accattgctg attgacaaag tccttcactg ctttaactgt
     1321 tgtcaggtca gcgagtgact gctgctggcc tctaacgtga gcacggagtt ccttgccatc
     1381 aggggtaaca aaacgccatt ctttttttgt cacaataatt tgtctggttg attctgatgc
     1441 caattgctct aaatcgacct ttgtcggtaa aacagccaaa accaattcac catctcgtcg
     1501 aatcagagat tgaccctgaa acgtatcttt ctcaatcttt ccttgggtca atgcttgata
     1561 ggttttgtct gtgataggcg acaaactctt agttacaatc cgttccaagg gatacgttgt
     1621 catgttagca tagtgtaacg taactggaat cattattaag gcgttcaaaa aaacaataat
     1681 aagaagattt tgccaccaag taaactgagc tcggctggca aacattgccc tagcagaaag
     1741 gcaatgtgaa atgtaagaaa taggaaattg ttttttatct ggcattaaac attactcctt
     1801 gtcaggttac cctttggtac caccacttgt taaaccggaa acgaaatttt tctgtaggaa
     1861 gaaaaagaga actgaaatag gcactgcaat cagaatagct cccgcagcaa ataaggttac
     1921 cttgggattt ctagcatcat tcgtaataaa cgattgaaga ccaactgcta ccgtataatt
     1981 ttcttgcgcc cttaaaagga atttagccaa catgaagtct ccgaaaggac ccataaaagc
     2041 ccataaggat tgtactgcaa tcattggtcg cacaagagga aggacaattt ggtagaaggt
     2101 cctaaagtgt ccagaaccat ctaacttagc agattcgtcc aagtcataag gtaccgtgtc
     2161 aaaataaccc ttcatcaacc aggcattcat aggaatacca ccaccgacgt aaatcaaaat
     2221 caagaaccag tattggttta aggcattaaa gagccacgcc ataacaaagt aagccgttaa
     2281 agccgccatg gtaggaacca tctgtacgac taggaaaaag attaaacttt ttttgcgtcc
     2341 aaagaaattg taacggctat acgcataacc tgtcaaagtg atgactgtta cttgtatgag
     2401 catggtgaag aatgccacga tcaaggtatt ccaataccat cttaagtata aggtatcttg
     2461 aaagagtgtt ttaaagttag acaaggtcca tggcccatca aaatgaaaac taaaggctgt
     2521 ggtatttcct ggtctaaaag ctgaactgac cgtcactagt attgggaata aaatcacgat
     2581 cactaaggag attagataga gataagtcaa taattgggtg aagaaacgtt tgcgtttcat
     2641 ggatattgaa acataggtct ttctcatgat ctctcctcca tatcaaaagc tttaaatttc
     2701 ttaaaggtaa tcaacgatac cgaaatcact attacagaga tgattaaggt cactgccgac
     2761 gccatcgaaa actgaggcga ggtttgtgtg gtcaacttgt aaatccaaga aatcagaata
     2821 tcagttgtcc cagctccgcc ccccacagta ccagggcccc cattattaaa cagataaata
     2881 atagagaaat tattgaagtt aaaggtgtac tgagagataa aggttggtgc tgcaaccgca
     2941 aaaatcattg gcatggtgat atggcggaat ttctgtaaga ctgtcgcacc atcaacggtt
     3001 gctgcttcgt ataaatcagc tggaattgcc tgtaaaatac ctgataccaa aatatagata
     3061 tagggaaatc ctagccaacc ttgcaccata ataacagcca cttttgtcca gaaggggtct
     3121 gtcttccagg acacaattcc gaagtcaacg aaaggaagga atttctcgat gaaaggaaga
     3181 acctgaacat tcatggcccc aatggaatca ttaaagaaat taccaaaact cataatagag
     3241 ataaatgctg gaacagccca tggtaacaag aatatcacac caaagagacg ttttcctctg
     3301 ataaaggatt gattagcaat aacagccgtt gcaattccaa taacaatttg aagacttgtc
     3361 gctgctaatg tccagatcag cgtccatgac aaaacagatg taaatgctgt tttgaaggtg
     3421 cttaattcta agatattaag aaaattcttc aaccctaccc aatccaataa acgatacgga
     3481 ggaatatgcc taaaatcgta attggtaaaa gcaatcagca aggtcactaa aactggaaaa
     3541 ataatagcga aagccattgc taaataagcc ggaatgatga gcaagtaagg aaatcctttt
     3601 tcataaatcg cttcaagggt ctctttagcc gtcaccggaa ccttcttgcc ttcattcatt
     3661 tgatgtgcaa ctaatttagc atctctcata gcaaccaaat gaaaaatacc aaataagact
     3721 agagtaatta attgcatggc ccccttaatt aacataaata aggagtgatc ttgcattgga
     3781 acacttccaa gactgtaaaa acctgttaaa gcaggaattc ctagaactac aatttctagt
     3841 agttctagga agaaaacaac taataagact ccccctttaa cgatttgttt attgtatagt
     3901 tgtcccaaac ctgggataac agatagccac atggctttat taggcttctg ttgtctttgg
     3961 ttatctacca ttttttattc ctcttctcat aaatgattta aggttatact ccttatcata
     4021 ctaacttcct tttaagctga ctaatttatt tttttatagt aatggtttgt gcttcatgac
     4081 ctgtctttat actataagtc tcaggacttg tccataaaac attcccaata ctatcttttt
     4141 taacaagttt gactgctatt tctttattga ttggtagatg agtatcaaag aaccagtttg
     4201 ggtacttggc aatggtctga gtgttattaa atagaggacc aacagcattc ttagcgtcat
     4261 ttgcccccat ctcaaaaaca tcacccatga gatatagttg ttcccctggt actgtcttaa
     4321 aatcattgat gagtaaacga acaggaattt gtttatcagt caatacttca aaggcttggt
     4381 aactattgct ggtttgctta tcggcagttg tcactgaaat gttataataa tttgctggaa
     4441 ccgtcggtac ttttaaggtg ataagggtat ctgaccaaga aaggatctca gcagagattt
     4501 ctccaaaagt cacttgacct ttagaattac caaaaccttg acctgaaata gtaatcttat
     4561 ttccagcaat acccactgaa gcatcgacat ctccaagttg tggtgtcttg tcctctcctt
     4621 cataagtcca tacagcgact tgccccgctc caagttcaaa gctactaata gtgccatctg
     4681 ctccaactat taattcttga ccgccaagaa gaccttctaa tttgtccttg tagctatttt
     4741 ggggcaaggc tgttttagca ttagtaattg tataaccatt cgtttgatct ctattaatag
     4801 ccactagtgc gacatgatta ccaaatttac gctcaaatac caaaacatga tcactaatcc
     4861 aacgttgttc agttgttcca taagctaaag cttgattttg ttttcttaaa ggagctagct
     4921 tactaatcac tttataggct tgtgactctt tattaaaact tggcatatca ccacgattat
     4981 taggatcttt atcacctgtt gcatactgct ctgtaccata ataaatatta ggcacacctc
     5041 tagatgttaa aagcaaagca taggcttgat taacagcagt ttgattatta gccacttttg
     5101 ttgcgaagcg ttccatatca tgattatcaa taaaggtcac ctggtctgtc acttcattag
     5161 tcacctgatc tctattttca agaaccttgt aaaaatctcg catggtaaac gttgaaaaac
     5221 ctgtatacaa ctgtctaata gcattggcaa atctaaaatc taaaagcccc atcccactat
     5281 tgttagcaaa tgtcgtcata tcataatcat cgtcggtatg tcccgagaac cattccccga
     5341 aaacaaagac attatgtttt tcatagatat gactcaacca atttttttgc caaccttgtg
     5401 acatatgttt aacagcgtca actcggatac catcaacacc caggtctaac catttatcaa
     5461 tagcttcttt catgtactgg tcaactttcg gattaatgtt atttaaatca gctagtccgt
     5521 acattgaatg ataaatagaa ttttcataag tactaaaatc ggtccaagat tcatgattaa
     5581 aaatcttgtc tttatcatct gaaaatttac caactaattt accgttttta tataaagcgc
     5641 catcttcttt gaaagttgtg ccttcttttt cggctgtaga cgtatgatta ggagcaaaat
     5701 caataactac cttaatatgg tgttgatgag ctactttgac tagttgttga aagtctgctt
     5761 cagtgccaaa atgctggttt gttttaaaga agtccttagc ccaataccca tgatatgcag
     5821 cacttccatt agaaggatca atactgtcga tattttcaac aggagaagaa atccaaatgg
     5881 cagaaatccc catatctgtc aggtaaccat ccttaatctt ggcgatgatt ccttgccaat
     5941 cacctccatg gtatttttta aggtcatttt tatcaaaaac atcagtctta ccattattag
     6001 aggtattacc atcgttaaaa cgatctgtta caatctgata aatcgtatct gttgaaaaat
     6061 ctgctttgtt ggtgacagaa gcattatcgg cagcagaaac agttagtgga aatgaaatta
     6121 agccaagtaa aacagcactt ttcgttaata acttataagt tttgatatgt aattctctca
     6181 tcgctttttc cttttcaaat aaccttttag ttactaaaca caatatatcc atcctggagt
     6241 aattgttttt tgtttttagt taccccattg cttgtcaaaa tctgtcctcc ttccttaata
     6301 gaataagcac tctcaccatt gttaagaaca agtgaaatgc tttgctcacc taagctgcgt
     6361 ttcagaatga ggcagtcttc gtcataatca atgctaatag ctccttcttg aagagctggc
     6421 tgttggcgaa gagcaataat cgcttttact ttgctaaaga aatccttgtc ccgctcagca
     6481 tcatcccaag gaaaaccgcg acgcgaatca gggtcgtaac ccccttccat tgccacttct
     6541 gtcccataat acaagcaagg aatgcccata aagataaaga gcaatgctaa agcagctaac
     6601 aatttatctt tggaaccctt aacttgagtg aagaaacgat gcgtatcatg agaatccaaa
     6661 agattcaagt tcatgcgatt gacttgccaa gtgtttcgca tcaaaatgtt gcttaatttc
     6721 tcagccaatt gttttgatgt catggtttca aaagcaaagt aatccaaaca ggctttggtg
     6781 aaagcataat tcattatccc atcatactga tcccctgcca aataaggata ggcatcatgc
     6841 cagttctccc caattaaaat ggcatctgct ttttccgctt taaccgcctg acggaaacgt
     6901 cgccaaaaat catgagatac ttcatcagaa acatctaggc gccaaccatc gatacaaaat
     6961 tctttaatcc agtatcgccc aatctcaatc agatagtctt gcacgtctct atttgaggta
     7021 ttccatttgg gcatatagtt acatccagca aaggtttcat agtttactag gtccatggat
     7081 gggtgttcat catgagtcat aaaccaatca aaaaacttag actctttacc gtatctcaag
     7141 acatcctgaa attccacggc atcactgctg gcatggttaa agacagcgtc tagaataatc
     7201 ttgattccca tttggtgagc taaatcaatc aattcttgca aatcatattt agttccaaat
     7261 tgtggatcaa tagcgtagta atcactaata tcatatttgt gattactaat ggattgaaaa
     7321 attggcgtta aatagatgac agtaatccct aaatctttga ggtaatctaa tttttctgta
     7381 attcccttta aatctccacc agcaaaactt ttaggagtcg gtttttctaa ccaacccatg
     7441 ttgacataac tgtcaccttt ttcccaatcc ccacggttaa agcgatcaac aaagatttga
     7501 taaaaaaccg cgtcttttgt ccaaggaatg acttgatgaa catcaacagc attgatataa
     7561 ggcatttgga agaaattata aaacccattt gaaaaatcgt aactagtagt taacccatct
     7621 tcactaaaat aataggtttg accttcttct tcaattttaa aaatgtaggc caacctcaca
     7681 tcgtctaatg ttaaagtagt ttcataataa gcatgcgtat ggtctgtata ggcaactgac
     7741 atttccaatt cttcttggaa ttcatgataa ccatatttag gcccgtggac caaattaaca
     7801 gtaatacctc tatcttcttt ggccacgcgt aatcggatga caagttcctt ttgagatatg
     7861 gcaaagcaat acctcgaatc aggaatatgg agcattccgg caacattcat ttctacctcc
     7921 attaataaag tattgacgat tacttctttt tctttaaaac gaaaaaatag cagagcgact
     7981 catacttcta tatttttagt atctgtttca tcttcatcaa actaacagaa atagaagctg
     8041 ggcgtacttc tttatcccag cctcccttct ttgccttatt cagcgtattt ttgttcaata
     8101 gcttccttaa tagtttttgc tgcttctttt gcagctttag atgcttcttc cttaccagaa
     8161 gccacgttaa agaacatatt tgcacctggt tcccaaactt cagccatttc tggaatattt
     8221 ggcattggtt gtgcgctctc aaactgacta atcacagctt tcgttaactc attaccctct
     8281 ttagaagcat agttacgtgc tgttaagtta gctggaattt cttgtgtttt atcatagaaa
     8341 cgtttttggt tttcagcatt ggtcacataa tcaaggaatt tttgagcagt cgtttttcct
     8401 ttagaatagt ttgaaataac ccaagcttta ccacctgcga acggttggta ttttttacca
     8461 tttgtcaaag taggaatttc cattacccca aagttaacac cagcatcttt aaaggagcta
     8521 gctgcccatg gaccatcaat aatgacacca gcttttttag aaataaattg ttcagtgata
     8581 aaatcaccag cttttttagt gtcttgcatt ccttgaggcc aagtgccata ccactgtttc
     8641 gcgtagttta acccttcaac agttccgtcg ttacctaggc ctaaatcact tggtttggtt
     8701 cctttatcac caaagatata accgccataa ccggcaatta aaccataacc ataatagaag
     8761 tctgtccatt tcgccaagaa acctacagat tttcctggtt cagaggcaaa agcaaactta
     8821 gaatcttttt gtaagacttc taattcagta aagcttttag gtgcttgagg cactaaatcc
     8881 ttattataat aagttaccaa agtttcaatt acgtctggtg caccataagt tttaccatca
     8941 atggttacta atttcttaac cgtatcatca aactctttgc tgtttcctaa agtaacctca
     9001 gagatttgac cttcagttcc taagccacct acacggtcaa aaggtgctaa aaagacatct
     9061 ggtgaagctc ctgttggacc atccgttgat agcttatcaa gagtatccat cataccttct
     9121 tttttgactt taacggtgac tttatgctct ttttcaaact cacccttaat actcttaatg
     9181 taatcaacat atccctcatc aacagagact acaatagtct ttccgtctgt ttccttaacg
     9241 cctttaccac caccacttac gcaagcagtt aggggtagca ctgaagctaa ggataaagtt
     9301 ccaagcacag atgctttttt ccaattccat gacataacat gttcctcccg aaatggtttt
     9361 ttcttaaaca caaatcaaat catctatcta ctctcatcat attagcaaat aaaagataac
     9421 ataaggcatt taaaattaat aacagtaata gtttcgaaac ttaaacgttt aagttaagtt
     9481 tatcgtaatc gctttcattt gtcaataact tttttacttt tcttttaaga gcatatcagt
     9541 agtttttctg ctaattgaaa acaaggcatt tactgtcaca caagtctgtc ctttcttttt
     9601 tctacaactt tatttcgtca gcgtaaataa aacaaaagct ctataatctc ttgaatgggt
     9661 aaacccactg tagagattat agagcttttt gaatatcaaa aaagtcccat aaaatttata
     9721 atgaaaagca accaaactct cattagaaag actcttatgg aacatatcaa tcatacctta
     9781 caacacatcg gaattaaaga caaaaatatc actctaaaca atgttttgaa ggatgaatct
     9841 cctactgagc tcgttactac ttaccagaaa ggtagatagg aaatcctgat gaagagtgtg
     9901 tccaagggat tatcaattag gtcttagatt acaaaatagt aattcaggta ggctctactg
     9961 ttttcaacac tagttctgca atttgttttc gagcttgctt agcttctctt attggaaaaa
    10021 taacatacgc atgaatcatt ttttctgcaa taatcagctg attagcaatt cgttgtcttg
    10081 ttaattgatc atgtaaagac ataatgtctg ggtaaaaaat ttcttttgtc ccaacaaagg
    10141 tcgttatttc aggcaaatcc tcacaatcac catacaaagg actgacataa ggaagttgca
    10201 tattaccttg accacctgcc catagttcac ctatatcacg aagacgacga gcatctaggt
    10261 aagcttcttc tgcttcaaaa tccttgtagg cttgattttt aaaactaaca tctaaccaag
    10321 gagatataag aattaattgc tttggctgta gtgctgtttt agtatctcgt atatacttag
    10381 caaacgccaa agccatcccc ccaccagaag aatcaccaat gacactaact tgactcggtc
    10441 cattagacaa gcaaaattgc tggtaagctt caactaattt aggatagaca tcttgataag
    10501 tagcatacgg tactttagga aagttaggaa aaacaacctt ggcattagtc agctgtataa
    10561 gatgcttaac cgtcttaaaa tgaagttttt cgggtcgtcg taaatagaca ctgccataaa
    10621 gataataaat aacaggctga tgactttgct tttgatcatt aataataaaa acttgtatcc
    10681 cgttcacatc gtattcttga gttgaaaaac ctactatctc ttcaggatta tctagatgat
    10741 aagggatatt acgattacgc tgcgtctcaa cccccttata attatcaact tttttagata
    10801 acttatactt tctataagca aacattttct caaccaacca acttgaaaag gagcgctttt
    10861 gaccatattc aactatcaaa atacctagta tcactccaaa aaaaatcacc acagcaataa
    10921 tgaccactta ttatcctcgt tttctaatat tattttacta ttgcaagtct ttcctatttg
    10981 cccaaatttt actataaaaa taagggttca ttttatagtt gggcactggc tgcttcgttt
    11041 ctagaaatgg ttgcactttc ttatcaaaag ctaaccagcc attccaaccg agatggatgg
    11101 tatcttgcat aaagtaggac tcaccaccat cttttgagaa gtcagcaatg cgatgaaatc
    11161 cttgtgactt taactggaat tttattttac gaactgccgc ttgatactta tcttgattta
    11221 agccggtata atccgcccaa gctttattaa caggagttat aacaaagagt acatctgttt
    11281 ttcgtttagc aaattctgat aataaaagct gaaaatcatt gtattctggt gacgccaggt
    11341 aactataatt aacttggaaa ttcttataaa gattgtattt aggtgctatt cgtttacgat
    11401 aaaatgtatt tttaatccca aaacggttgt tggttgttgc taattggcct ctcttagttg
    11461 ctaacgcatt caattgttta tacgaaaata ctttaggtaa tcccttaacg cgaggcaaaa
    11521 ttcttttttc atagttagta gatttgccta aaaaactaaa aagggactct tctctcaatg
    11581 ctacttgatg ttgacatttc aaaatagctc tgtctaaccg actaagagac ttacccttac
    11641 ttactttttt caataagttt gatttagaca caccagggtt aagctcaagc aaacgctttg
    11701 ctgcaaactg tgattcttta tcagttctag cttttagtaa aaattcaatc acttgagtgt
    11761 tagacaagta catctgaacc gcactaggat taatcccttg agcagtaaac cattgaggag
    11821 atactacaaa gatggctttt ttcttttgca tttcattggt aatttgttgc ataccataat
    11881 aatgcgacaa agatgctgat cctctcttac caattaaaaa tggtctatag ctccgcttgt
    11941 agcgctctgc aagcaccgaa gggtgcatac tatccattcg actccattcg ctagaaccaa
    12001 aaaaaggcac aaaacgacaa gtctcatctg ataaagcttg tcttttaatc aaaccatttt
    12061 taaaagaact atctgtgatc gcaacggcat ttgctttttc ctgagctatg gaatgatcaa
    12121 gttgtgtagg aaaactaaaa atagtaatca ctactaaaac aaaggcaata agaagaggac
    12181 ctagaattaa ccagagtctc ttaagcatgt tgcaattcct ctacaccttg aacaatctta
    12241 gttactgtgt tccaatcatc acgaccaaat tctgaaatag gtactttaat attgaacgtt
    12301 gactctaact caacaatcaa ttcgactgtc ccaagactat ccaacacacc tgcatcaaac
    12361 aagtcttcgt ccatcatctc tgaaacatct tccataaaca aacggtcaaa caattcaatt
    12421 acagtttctt caatagacat ttttatactt ccttttatta atgtagtggt ctgttaaacc
    12481 aaaggtcatt taaaaaacca gagaataaca gaagtgaaaa catgacaacg tggaaagtta
    12541 ccacgataca taagacatgg aatgccctgc tttgaaagag gggtgataag ccttttttct
    12601 tacggtgacg gttgatttct tttttcttac gaatccaagc atcattaata ataagtccta
    12661 taccatggaa aagcccataa gcgatataat accaggttaa gccatgccaa aatcccatga
    12721 ctagcatatt aaccagatag gcaacacctg aagtgacatt tcggttttta aaggttttat
    12781 gcttaatcaa tagatgaacc aatcgcataa acacataatc tctaaaccag aaggacaaac
    12841 tcatgtgcca tctattccaa aaatctttca agcttgcaga taagaaaggc atgttaaagt
    12901 tttctggtgt tcgaataccc aataggtagg aaataccaat cgcaaacatc gaataaccag
    12961 caaagtcaaa aaagagattc aagccataca cgtacataac cagaatagtt tccttattaa
    13021 agtagccacc aaccatcaaa gccttatttt caaggagagg aagtaagatg ccacccaaac
    13081 aataggaaat gatatgcttg tataggaaac ctaacatgag atacatgact gccttattga
    13141 gcattgcaaa ataatcatca cgttcaggaa gtttatgata atcttcctga aaacgtctga
    13201 agcgatcaat ggggccactt gagaaagtgg ggaagaaaat catgaagcga ataaaatccg
    13261 gtaaacgaac ctcttggaga gtcccgtcac gcatctcaat aatcattcca atacttttaa
    13321 aggtcaaata tgaaatccct agaaaactaa agagtgtttg agaagaagga cctgttaggg
    13381 gagcaacttt gacccagata agggggaata aggccatcgc aatagctaag taaaacactc
    13441 ctgcactatt agctttctca cggtatttct ggtaagcaaa aacactgatg atttgccaca
    13501 aaagataaaa gagaaaagca actaattgtt gataatgatc tcctccaaac ataaagagaa
    13561 taaataccaa acttaccaag gtttcataag tgcttaagcg cttttgttta aaaatcccaa
    13621 tgatgactgg taagaaagcc aaaataagat agacaaagta aatggggtta ccgtaaggtt
    13681 ccatgtaggg aatgtggcta aaaaacatca tcatcggtta ttgacctcat taatcaaagt
    13741 tttaatatct atcttaccat ttggagttaa tggtagactg tctcgataaa gaaacttaga
    13801 aggcatcata taactcatca tatgatcctt aacagaggct ttgatagctt tggttaactc
    13861 caattcccta tcaaaacgct ctttaacccc atctttgaca acgatatagg ccaagagatt
    13921 ttgaacctta tgttctttat tgtaccttgg aacagccaca gccgatgcta ccattggtga
    13981 ttgattcaac tgttgggaaa catcttctag ttcaatacga taaccagcat atttaatctg
    14041 aaaatccaaa cgcccaccat acagcaaaat attatcttct gtcagtgagc caatgtcccc
    14101 tgtatgataa gcaggctgtc ctttaaaagt aaagaaagct tctgcggttt tctcaggatt
    14161 gttgaggtaa ccttttgaca cagcaggccc tgtaacaata atttcaccct gctcccctga
    14221 agctaactcc ttaccatctt catcaataat ataggtcgga gaatctggtt tggggtagcc
    14281 aatcggcaag cgggtataat tatccaccat ttctctggta atttcaatag cggataaggc
    14341 tacagtcgct tctgttggtc cataggcatt gatgatcttg gcactcggga aacgttcaaa
    14401 gagttttcga gccgtagaaa cggttaattc ttcgccatca aaataaaaat gcgttaaagc
    14461 tggcatctta gcctggcaaa aatcatcgct aagcatggcc atatcagcaa atgaaggagt
    14521 cgatgtccaa atgccaactg gaagttgcgc aatcgttgta aataactgtt taaagtctgc
    14581 caccaattct tttggaagtg caaataaggt tccacctaaa gccaaggtag gtgcccagta
    14641 catgacagaa aggtcaaagg aatagggtgg ctgcgccagc atttgtggct gtttaggtac
    14701 atcaaatgct gcatcttcga tcatccagtt ggtaaagctc aaaagattgt catgtgaaat
    14761 ttgcacccct tttggttggc ccgttgttcc agaggtgaaa ataatgtaat aattatcatc
    14821 tcctttaaca gaatgtgttc gctcataggg catttctgct aattttgctg actcaatctc
    14881 tgatagtgag acaagggaaa tcccttcaat agtaagaggg aattcctcaa tagcaatgat
    14941 taaactgggc tttgcaatct caataatcgc taaaatacgt tctggtgctg aatgcacatc
    15001 tactgggata taggcatgac cagatttggt taaagctaca aaagtagcta acatatcata
    15061 agtttgcgcc ccaaaaacca aaactggaga ttttgccagt aaagctaagc tatctataaa
    15121 tgcagcaatg ctatcagaat ctcttttgag ttgtccgtaa gtgcggtgtt cccctaaaca
    15181 atcataaact gggaaatcag cctgtgtctg agcaaactgc tcaatactgt caatcatatc
    15241 cttaatcatc agtcaatctc ctaaaattca ttataaataa agttactttg accatgtcct
    15301 agataaccaa agaaatagac cagcaacatt aaaatcagat aaaataaaat tgtcttgcca
    15361 ataaaaacag caattcctct cattcttgat ttgtttttta tcatacttta ccttcttgta
    15421 ttagtgaaat aatgtcctaa aaaattctac tcgaaaaaac ctaacttgac aagctaaaag
    15481 tcatattatt aaaaagaaaa tttttttaaa gcttaactat ttagacagtt aaattttatg
    15541 cctttttact aaacttacta agttaatctt tactttaaaa agttaactta aaattgtcct
    15601 aataaaaaga attatttgac tttaacttat atatgttaat aagtataata taagctttat
    15661 tcaaaaccta gtccattaat ttaagttcta aaatcaagtc tcgcatttga gctgccaatt
    15721 cgaaatcaag taattcagct gcctcttgca tttgtttttg cagagcattg atcgcttctt
    15781 ttcgttctcc tcttgacata ctttcataat ccatctcttc tttcgatata tcattgtggc
    15841 ttgttttact tatactaata aggcctctga tgtctttttt tatagtttgt ggaacgatgc
    15901 cgtgcgcttt gttatatgct atttgaatct cacgacgacg agccgtttcg tcaatagccc
    15961 gctgcataga atctgtcatc ttgtccgcat acataatgac atgaccatct acgtttcgag
    16021 ctgctctacc gatggtctga atcaagccgc gttcattacg gaggaaaccc tctttatcag
    16081 catccaaaat ggctacaaga ctaacttctg gcacatcaat cccttcacgg agcagattga
    16141 ttccaatcaa gacatcaaaa acaccaagtc gtaaatcacg aataatttct gtccgctcta
    16201 aggttttaat atcactgtgc atatacttga ccttgacacc catctctttt agataatcag
    16261 tcaaatcttc tgccattttt ttagttaagg ttgtgatgaa cgtccgttca tcgcgcgcta
    16321 cgcgctggtt aatttctccc aacaaatcat ccatttgccc catacttggc cttacatcaa
    16381 tttcaggatc aagtagccca gtcggacgaa taatttgctc aataattgta ttagtctgtg
    16441 acatttcgta ttcaccaggc gttgctgaaa catagataat ttggtgcaca tgactttcaa
    16501 attcctcgcg tcttaaggga cgattatcaa gagctgatgg caatcgaaaa ccatagtcaa
    16561 ctagcatttg ttttctggct tggtcaccat tatacatgcc tttgatttgt cccattgtca
    16621 tgtgactttc atcaatcata attaaaaaat cttctgggaa gaaatctaac aaggtataag
    16681 gaggttctcc tggtgacctg ccatccatat gacgggaata gttttcgact ccgctagtat
    16741 agcccatttc acgtaacatt tcaatatcat attccgttct ttggcgtaat cgctgtgctt
    16801 ctagtagttt accttcagat tcaaataact gtaactgctc tgctaactct gcttgaattt
    16861 tggctattga ttgctccata tgctcatcat ttgttacaaa gtgagtagct ggaaataaaa
    16921 ccaaatggtc aacttctcca atagttttcc cagttaaact ttcgatttca catatacgat
    16981 caatctcatc cccgaaaaat tccacccgaa aagcatgttc atccctggat gctggaaaga
    17041 cctcaacaac atcgccacgt acacgaaaac agccgcgttg aaaatcaata tcattacgct
    17101 caaattgaat atctactaat tgatttagca aagtgtcacg tgatatctcc tgacctggac
    17161 gtaggctgac tgctgaatct gcgtactctt ttggcgaacc tagtccataa atacaagata
    17221 cagaagctac cactatgaca tcgttacgct caagtaaaga agaggtggct gaatggcgaa
    17281 gtttatcgat ttcatcattg actgaactat ctttttcgat ataggtgtcg cttgatggga
    17341 catatgcttc aggttggtag taatcataat aggaaacaaa gtattccaca gcattgtctg
    17401 gaaaaaattc tttaaattca ccatataatt gtcctgctaa ggttttattg tgagctatca
    17461 ctaaggtagg tttatttact ttactaatta cttggctcat ggtgtatgtt tttcccgttc
    17521 ctgtagcacc taggagaatc tgagcttttt ctccgccctc aatattatca actaggcttt
    17581 caatagcttg tggctgatca ccagacggtt tatactttga ttttagttta aatggtttat
    17641 catcacgttt atcaatcatt ttataccctc taatagtctt atctcttata gactatttta
    17701 acatagataa gccagaatca cctccaataa gcgtaaaaaa catgtcattt acatgttatg
    17761 ttttatgaca tataaataac aaatttcttg cttaatgttc aacattttgt tataattaac
    17821 catatttttt agttttataa ggagattagt atgacgcaca aaataaaagt attgctgctt
    17881 gcgataatgt ctattttttt gacatgcaat attgcaagtg ctgaaactat tgctattgtt
    17941 tcagatacag cttatgcccc atttgaattt aaagactcag atcaaattta caaaggaatt
    18001 gacgttgata ttattaatga agtagccaaa cgtcaatctt gggatttcag tatgagtttc
    18061 ccgggttttg atgcagctgt aaatgctgta caatctggtc aagcgagtgc tctaatggcc
    18121 ggtacaacca ttacgaatgc tcgtaagaaa gtctttcatt tctcagagcc atattacgat
    18181 accaaaattg tcattgcgac acgtaaagcc aatgccatca aaaaatacag tgacttaaaa
    18241 ggaaaaacgg tcggtgttaa aaatggaaca gcggctcaag cctttttgaa taactataaa
    18301 aaaaagtatg attatactgt taaaacattt gacacaggtg atcttatgta taatagttta
    18361 tctgctggtt ctattgccgc tgttatggat gatgaagcgg ttatccaata cgcaatcagc
    18421 caaaaccaag atattgctat taacatgaaa ggagagccca ttggaagctt tgggtttgct
    18481 gtcaaaaagg gaagcggata tgattatcta gttaatgatt tcaatacagc tcttaaagct
    18541 atgaaagctg atggtaccta ccaagctatc atgaccaagt ggttaggcac agatgataaa
    18601 gctaccacca gtcaggcaac gggaaatcca tctgccaaag ctacacctat aaaggacagt
    18661 tataaaattg tctctgattc gtcttttgca ccttttgaat ttcaaaatgg taagggcaaa
    18721 tacgttggta ttgacataga attaatcaaa gctattgcta aacaacaagg tttcaaaatt
    18781 gaaatcgcta atccaggttt cgatgctgcc ttaaatgctg tgcaatctag ccaagcagat
    18841 ggggtcattg ctggtgcaac tattactgac gctcgtaaag ctatctttga tttttctgat
    18901 ccttattata cttctaatat cattttagct gttaaagctg gaaaaaacat caagaactat
    18961 gaagacttag acagaaaaac agtcggtgct aaaaacggca cttcatctta ctcttggtta
    19021 aaagaaaacg ctcctaaata tggttataat gtcaaggcat ttgatgatgg ttctagcatg
    19081 tatgatagct taaattcagg ttctgtagat gctatcatgg atgatgaggc ggttcttaaa
    19141 tacgctattt ctcaaggtcg tcgctttgaa acacctcttg agggcatttc tactggtgaa
    19201 gttggttttg ctgtcaagaa aggaactaat ccagaattaa tcgaaatgtt caacaatggc
    19261 ttagctgctc tcaaaaaatc tggtcagtat gatgacatta tagataaata ccttgactct
    19321 aagaaagctg caactccttc tgaaaaaggt gctgatgagt ctactatttc aggcctatta
    19381 tcaaataact acaaacaact attggcagga cttggaacca cgctcagttt aacccttatt
    19441 tcatttgcta ttgctataat tatcgggatc atctttggga tgatggccgt gtcaccaact
    19501 aaatcacttc gactcatttc aacggtcttt gtagacattg ttcgagggat tcctttgatg
    19561 attgtggctg ccttcatttt ctggggagta ccaaacctta tcgagagtat gaccggccac
    19621 cagtcaccga ttaatgattt cttagctgct acaattgcac tgtcacttaa tggcggagcc
    19681 tatattgctg aaattgttcg cggtggtatc gaagctgttc cagcagggca aatggaagct
    19741 agtcgaagtc ttggtttgtc ttacggaacc acgatgagaa aagtaattct cccacaagct
    19801 gtgaaactaa tgttacctaa ctttatcaat cagtttgtta tttcattgaa ggatacaaca
    19861 atcgtctcag caattggttt agtggaactc ttccaaacag gtaaaatcat tattgctaga
    19921 aattaccagt cgttccgtat gtatgctatt ttagcaatta tttaccttat catgattaca
    19981 ctcttaacaa gacttgcaaa acgtttagaa aagaggctta actaatgaca gaattaaaaa
    20041 ttgatgttca agatttacac aaatcttatg gtcaaaatga agtcttaaaa ggaattgatg
    20101 ccaaattcta cgaaggtgat gtcgtttgta tcattggtcc ttctggttct gggaaatcaa
    20161 cctttctacg taccctcaac cttctagaga cgattaccag tggtaaggtc gtggtggacg
    20221 gttttgaatt atcagatcct aagactaata ttgacaaggc acgtgaaaat atcgggatgg
    20281 tattccaaca cttcaacctt ttccctcaca tgactgttct tgagaatatt atctttgctc
    20341 ctgttgaatt aggtaaagag t
//
LOCUS       DAUVUY010000028        20046 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.028, whole genome shotgun sequence.
ACCESSION   DAUVUY010000028 DAUVUY010000000
VERSION     DAUVUY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20046
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.028"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(299..1744)
                     /locus_tag="VOX58_000873"
     CDS             complement(299..1744)
                     /locus_tag="VOX58_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HES5878116.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            complement(1825..2751)
                     /locus_tag="VOX58_000874"
     CDS             complement(1825..2751)
                     /locus_tag="VOX58_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HES5878117.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            complement(2761..3711)
                     /locus_tag="VOX58_000875"
     CDS             complement(2761..3711)
                     /locus_tag="VOX58_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HES5878118.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIVFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLVGSG
                     ADPIKWYSNPTWWPLILLFMSGWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            3907..4784
                     /locus_tag="VOX58_000876"
                     /pseudo
     CDS             3907..4784
                     /locus_tag="VOX58_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(5395..6837)
                     /locus_tag="VOX58_000877"
     CDS             complement(5395..6837)
                     /locus_tag="VOX58_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HES5878119.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLALLASLGHNSYRTSIQWTRLIDDFEQVTINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRQGPAELSDGR
                     IGTILNLTPAYPASQSEADMAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDAYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIRF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(6862..8556)
                     /locus_tag="VOX58_000878"
     CDS             complement(6862..8556)
                     /locus_tag="VOX58_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HES5878120.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDMPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAELTVKHFFNRQLDKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFAMRGIIEGFYGTPWTREERLDCLRFIGRKRM
                     NTYMYAPKDDDYQRKLWRDLYPEDWVTYFKELLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQSDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSNSWLETLTDYTGDAELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVDELVERVNTLRKLDKPAFQQAIVPWFERVAKDQDFWQAI
                     LEQGPHLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            complement(8607..9647)
                     /locus_tag="VOX58_000879"
     CDS             complement(8607..9647)
                     /locus_tag="VOX58_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HES5878121.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAEKQVTSPEANKDLLLILPFTSDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LYIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            9780..11066
                     /locus_tag="VOX58_000880"
     CDS             9780..11066
                     /locus_tag="VOX58_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HES5878122.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQMKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANGKGHQTDRTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTILSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSITP"
     gene            11081..13786
                     /locus_tag="VOX58_000881"
     CDS             11081..13786
                     /locus_tag="VOX58_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HES5878123.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDP
                     DFHSFHLDGQTIILDDYLQVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDRWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDNSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLSDLANDLPDNL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLTQNHPHDSICGCSVDSVHREMINRFEKAYEVGHYLAKEAAQQIAGAV
                     NTSDFPTDSQPFLLFNTSGHSKTSVTELTLTWKKYYFGQRFPKEVYQEAQEYLAGLSQ
                     SFQVIDVSGNTISEADILDTSIAFDYDLPKRTFREPYFAIKVRLRLPITLPAMSWKAL
                     ALQLGNETAPSATVPLYDDSNQCLENEFLKVMIQTDGRLTITDKQSGVSYQDLLRFED
                     CGDIGNEYISRQPTHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPISADKLLQA
                     EMEAVIDITERQARRSQEKAELTLTTLIRMEKNNPRLQFTTRFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPDATFWKNPSNPQHQECFVSLFDGENGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPILVEQTSHHKGSLRAEHSYVTGADDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(13887..15242)
                     /gene="rocA"
                     /locus_tag="VOX58_000882"
     CDS             complement(13887..15242)
                     /gene="rocA"
                     /locus_tag="VOX58_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HES5878124.1"
                     /translation="MLEDFLQFLGFIFLDIIEIMLTLKLFSFVSAIPLRLKNIFYLSL
                     SMVLFQVVFWAFFPDHFILDVVMLAQFLFFALIALYYGKSIKAKFLMFYAFFPLVSIS
                     LVKRFIVFFVMPLFGMPYSVVKHNTLLIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQ
                     SHRASKLLVFTNSSMALYYLCVQGIDVMSPSLSGLATTTARSIIVLFYFILFLTLLIH
                     LERYVKQNSIEAIVQQKEYRELINYSQHLGLLYQDIQELRQLLTTVSSRLKIGIEQND
                     ISIVRLTYEGILNAEKNNAKDDRLDLTCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVS
                     IPNCIATFFLEVVDFTKLLSFLLDNAIEMSLETKQPCLSIAFLDQNHKLVIVIQSSTK
                     QGQNDSQSVFATPALKKRDDWQFDLRNVTTILNRYDYLTISSQIHDGILTQLIEIAKP
                     D"
     gene            complement(15907..17262)
                     /gene="rlmD"
                     /locus_tag="VOX58_000883"
     CDS             complement(15907..17262)
                     /gene="rlmD"
                     /locus_tag="VOX58_000883"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HES5878125.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITSVKRNFAEAKLLMVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLANFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
     gene            17377..18153
                     /gene="recX"
                     /locus_tag="VOX58_000884"
     CDS             17377..18153
                     /gene="recX"
                     /locus_tag="VOX58_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HES5878126.1"
                     /translation="MKITKIEKKKRLYLIELDNDDSLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEDLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            18233..18766
                     /locus_tag="VOX58_000885"
     CDS             18233..18766
                     /locus_tag="VOX58_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HES5878127.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            19187..19550
                     /locus_tag="VOX58_000886"
                     /pseudo
     CDS             19187..19550
                     /locus_tag="VOX58_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19846..19917)
                     /locus_tag="VOX58_000887"
     tRNA            complement(19846..19917)
                     /locus_tag="VOX58_000887"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19882..19884),aa:Glu,seq:ttc)
     gene            complement(19940..20013)
                     /locus_tag="VOX58_000888"
     tRNA            complement(19940..20013)
                     /locus_tag="VOX58_000888"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19977..19979),aa:Ile,seq:gat)
BASE COUNT         6260 a         4379 c         3633 g         5774 t
ORIGIN      
        1 tcctcttttt cttgttaaca cttatagcgg tttcaaggac cagcagctac gagactagtt
       61 ttagcacccc cagtaagatg ctggtattag ttttactctg attaatagtt tagactcttc
      121 taatatagca cactttatag gcttttggaa accgctttac taagagtagt aacattattt
      181 acattaattt ttctcccttt ataacttcca cttatttgat gatccgtttt ttccactcat
      241 cagaagaaaa gagctgagat ttcccagccc tcaacttata atcaagaaaa tgaatgcctt
      301 acttgtcttt agctacaaag tcgtccaatt gtttttgaac ttctttttgc accttgtccc
      361 aaccagcacc tttgaggtct gcaagaagtt ttggaagggc ttcgtcagga tccactgtac
      421 cagtattgat acttgcttta tatcggttca tgacatttga aatatttgat aattccgttt
      481 tgataacttt tgtgtcaaca gtgaaaccaa ggattggaga ttctttagca tccttgattg
      541 attggtcacg tttagcaatc atgtcgtcag taacagattc ttgagtgtaa aggattttat
      601 tgttaccagt attccaagca cccatgtgca tttttggttg atagccatcg agcagtttga
      661 ttttcttgtc gccaattttt tcccaagctt tgccttcgac accataaaca agcccattta
      721 acaattcggg atcgctatta agaagagaaa ggacttcaac tgctttttct ttgtttttag
      781 atacgcttga taccacaaag tttgccattt gtgcttgtga tgtggttttt agcggtttag
      841 tcaatggacg agacacgatg tcttttcctg cagcattggt caagatagta tcgccatagt
      901 ccataggacc ttgggtttct tcacgcataa accaagtgtt tccttcaagg ggataacctt
      961 ctgtattggt cgctgcatct gttggaatca aaccttcttt ataccatttg tgcatcaagc
     1021 ggagattgtt tttaaaggac tcatcttcat actgattaat gatggttggc ttgccttcat
     1081 caattttcac agcaaagggt tgggttttgg ttaatgggta gtcatagtca cctgacatac
     1141 taaagacttg accaatagca aaagctgctg tatttggttc tttttcgtgg aattgtttca
     1201 agacattttc agcatctgca taggacttaa tgtttgaaat gtcaaggcca tatttgtcca
     1261 ctagttcttt attgaaagaa agcatctgtt gggcataaac gttggcatct actgggaagg
     1321 catagagttt accgtcaata gtatttcctt taatataggc tgggtctaag tttttatacg
     1381 ttttcttagc gtattttggc attaacgttg tcaaatcagc aaaagcacct ttttgtgcat
     1441 tgaccacata attattggca aaagcaatgt catagttttc accagaggca atgatggtac
     1501 tcattttatc atcccagtcc ccccaaccga tgtattgaag gtcaaccgtt gcacctgttt
     1561 tttctttgat gcgcttgtta gcaattgtca tcaattcatc gaagttatca ggtttgtcac
     1621 caacttggta catcaataat ttgacatcac tatcctttga tgctgacttg ctttcgcagg
     1681 ctgctaaact agacgctgca agcacagttc cagtcacaca cacgattttt tgccactttt
     1741 tcataagtga agcctcctaa aaaatgtttt cttttgttac taatgattag ataaaatctg
     1801 gtcttgtgac cagttcctta agtcctattc cttcacccca ccaatggtca aaccaccgac
     1861 aaagtacttt tggaagaatg ggtaagtcaa ggcaatcggc aaggttgcaa taaccacgat
     1921 agccatacga actgattctt ttggcagcga tgctaagcta tctgccattt gtgcactcat
     1981 gccagcattt tgtgccaagg cctgtaggtt gctctgaatt ttcatcagta agtactgcat
     2041 tgggtagagg ttttcacttt gcacataaag caaggcgtta aaccaatcat tccaataagt
     2101 taaggcagaa aataaactga tggtagcaat ccctggaaca gccaaaggga gaacaatttt
     2161 cataaaaatc atccattcac tagccccatc catacgagcc gactcaataa tgctatctgg
     2221 aactgtcttt ttaaagaacg ttctcatgac caagatgcca aatgggccca gagccattgg
     2281 taaaatcaaa gcccaaaccg tatccttcaa atgaaggaga ttactgacca ccaagtaatt
     2341 agcaaccatc cctggtgcaa agagcatggt gataagtgca taaacagtaa aaaaacgtct
     2401 gtatgggaaa taaggtcttg aaatcgcata agcataggtt gatgtcatgg tcgtgttaat
     2461 aaaggttccc accactgtaa taaacacgga tatcccaaat gacctcatga ttctatggga
     2521 catggctcct tgaaaaatat actggtaagc cttagttgac cagacatctg gaaaaaaact
     2581 atacccatga ttaatcaagt aactttcatc agtgaaggaa ataataatca cgaagataaa
     2641 aggaatgata caagagatgg caaacagacc aatcagaata ttaaagatag cattggtttt
     2701 tcgatcaaag gttctaacat taaccttttc gacttttttc tttttctcca tgttttcctc
     2761 ctaaaacaag gctgcatcag gatccatgcg acgaacaatc gcattagtga ctaacaagat
     2821 gctggtccca actactgact gataaaggct agctgccgat gccatcccaa tatctcctgt
     2881 agcagtcaag gcattgtaga cataggtatc taacacactg gtcacgtcat aaagtgggcc
     2941 tgaattcttg ggaataacgt agaaaagtcc gaaatcagat ttaaaaatat taccaatatt
     3001 aatgatcaat aagatagaca tcattggcaa taattgtgga atggtgatgt tcctaatttg
     3061 ttgccatttg cttgcaccat ccaccatagc agcttcataa taagtatcag aaatcccttt
     3121 gactgaagca tagtagataa tgctattgta gcctagccct ttccagccac tcataaagag
     3181 taaaatcaag ggccaccaag tgggattgga ataccactta attggatctg ctccactgcc
     3241 aaccaagagc tgattaatga aacctttatc tgtatttaaa aaggcagaaa caaagtattc
     3301 aataaccacc catgataaga aataaggcaa caaggacatg gtgtgataga ccttaaccgt
     3361 tcgcttattt cgtaactcac tcatgataat ggcaaaaaca atcgcaaaga aaacattaaa
     3421 aagtaagaaa atcacattgt aagcgatggt atttctggta atcaaccaag catctttgga
     3481 agcaaagaga tatttgaaat tagccaaacc aacccaaggg ctctccttca aactttccat
     3541 aaagccccct gctgaataat gaaaatcttt aaaagcaacc acattggcta aaactggaat
     3601 ataaaagaaa aagatgaacc agatgaaacc tggtaatacc attaataata aggcccggta
     3661 tttaatcaca tttttccaaa aggatgtctt tttgttggtt tgtcttgtca tttcccaacc
     3721 tccctttgtg ttaatcacgt tatagttaca gtatatcaag cgctttcatt attttaaagc
     3781 aacaaattat aggtatgata acacaaagta tagaatcact tagaaagcct tttcattgtt
     3841 gcttaaatct gtcatactaa gatgagacat gatcgtctat tctactataa taaaggagaa
     3901 cacctcatga gcttgttatg tattgatata ggaggcactt cccttaaatt tgccctctgt
     3961 cacaatggcc aattaagcca gcaaagcagc tttccactcc ctctagtctg gaaaagtttt
     4021 accaattact cgaccaagaa gttgctcgtt actctgctta ccatttctca ggtattgcca
     4081 tcagttcacc tggggcaatc aataaagaaa agggggttat cgaaggcgcc agcgctattc
     4141 cctacattca ccattttaaa atacaagagg ccttagaaga acggctacat taccctattt
     4201 ctatcgaaaa tgatgccaat tgtgccgcct tagctgaagc taccttaggg gcaggaaaag
     4261 gcgctagtag cctagccatg ctcgttcttg gtactggagt tggaggaagc ttggtcattg
     4321 atggcaagat ttaccatggt gcccacttat ttgggggcga atttggtttc atgatcatga
     4381 acgaccgcta ccaaaccttt agtcagttag ggacagtggt aaatatggcc aagcgctata
     4441 gtgcaatcgt caataacgga aaagattata ctggcaaagc tgtccttgcc ctcgcagaac
     4501 aaggagacca cctagcactc aaagaaaggc aggtctttct ccaaagttta gccatcggta
     4561 tttttaacat tcaacacgcc tttgatcctc aactgatttt aatcgggggt ggtgtttccc
     4621 aagctgactt tctactgtca gcgattgaag ctgaactaga caaactctac caagccgtcg
     4681 aaatctccga ccttagaccc caattagcta tttgtcactt taaaaacgaa gccaatcttc
     4741 ttggagctgc cattgatttt taccaagaac acaaggaggc ctaaccatgg accgctaaat
     4801 ccctcttatc ccctattgtc cctaatcgcc tctgacatct acaaagacat cctaaaagga
     4861 ttgctgatta gctacaacga tactcgcttt ctgcctaaat gactctctgc tgatgaacac
     4921 agcctgatgc ctggtccctt tgttgatgtc attgttgctg acgcttctgt taaaggcatt
     4981 ggtcatcagt taatactcga gctattagaa gctatgctag ttatcgccaa taagcaaagc
     5041 ctcacttaat aactcactca tatagtcccc ttatcgtgga cagtgacata acaaacagtc
     5101 actagaaacc atgaggggct tttatctggt ccaaaaatat gaaagaaaat cctaaaatac
     5161 caagatgaaa aacaagcatc cttagccaag atactctaaa gaattgaaga gccaaattac
     5221 ccaagatgga tagatgatgc caaaccagac agaacgctta tatctagttc ggcaaatacc
     5281 aacaccttcc aaagaaatac aagagcctcg atcctcttaa catgaggaaa aaggcccttg
     5341 tttaatcatt ggtaacatca ttattcactt gtctcaaagg agacgttcac acgttcaatc
     5401 actgaggtga gaagcagagc ctccaaactc ctccattact tctaatgaaa tcaagcggtt
     5461 atgagtagct acttttttaa accaataagc tgaagcttta ggtcgtcgca cttgggtatg
     5521 gatattattt tccacaaggc catagcggtt tttataggca ttgagccaag accagccatc
     5581 aattggcgtc catacatggt atccaaaaca attcgagcct gcttctattc ctttgtggag
     5641 gtaggtcaaa tgttctttca aaaatcggat gcgataatca tcttgaatct gaccagtttc
     5701 atcacggtaa cggtcttctc ctgaaatccc tacaccattt tcagataaga accaaggaat
     5761 attgtcgtaa tggtctcgca tcttgatggc aatatcatag acagcctcag gatagatttc
     5821 ccacccctta tccacgttca tgcggcggcc aggcatcagg taggcgtcat aataccattc
     5881 aggactccaa gatgggctaa tcactggaat gacatccgga gccttcacgc gcttagggtg
     5941 gtaaaaattc aatcccaaat aatccactct attctcagca ataagagcca attcttctgg
     6001 cgttgactgc cacaaaaccc catctttttt caataccgca accaattctt ctgggaattt
     6061 cccatgaaca gcagcttcca taaacaagtc attgttccaa agttctgcaa aatgcgcagc
     6121 agccatgtcc gcttctgatt gactagctgg gtaggctggc gtcagattga gaatggttcc
     6181 aatacgccca tcactaagtt ctgctggacc ttgcctgtaa gcttgaataa ccttggctgt
     6241 ggctagggct aggttatagg cgacctggac tgcttttttc ccatccacga tagcagggta
     6301 atgaaattgc attaaataag agccttccac aacgaccata ggttcattgt ggacaaacca
     6361 atccttaacc cgatcaccaa actgctcaaa acaaaccttg ctaaaggcga caaataagtc
     6421 cacgacatgc tttgattccc agccaccata ggcttgatag agggcaatgg gcaggtcaaa
     6481 atgatgcaaa ttaatgacag gacgaatacc attggccaag caagcatcaa tcactcgatt
     6541 ataataagca aggccatcag gattaatcgt cacttgctca aagtcatcaa tcaaacgcgt
     6601 ccactgaatg gaggtgcggt aggaattgtg acctagtgaa gctaacaagg ccaaatcaga
     6661 ttcaatctga tgataggcat ccgaagccgt atcaggtccc acgtaatcgt aaaacaagtc
     6721 aggctcctct tcataccaat aatcaaagag attgcggtgc tgtttagcaa atcgcccctc
     6781 actttgaggg cctgaagtgg ctcctcccca ccaaaactct tttggaaatg ctaacatatc
     6841 attcctttct agctcgtatg actaagcata atcttcaggt cttgcttggg taacttgacc
     6901 ttgattagct gttagtttat cttgctgttg atagtagatg cgatagtagc gacttggaat
     6961 gtcacttcca atacggtgtt tatcttcttg taagtcagca tagagtgttt ccaaatgagg
     7021 cccctgctct aaaatggcct gccaaaaatc ttggtctttg gccacacgtt caaaccaagg
     7081 aacaatagct tgttgaaacg ctggcttatc tagctttctc aacgtattga cacgttctac
     7141 caattcatct acccaagctg ataacgttga tacatcctta gccaggagag cttcttccac
     7201 ttcaaaagga aggtccctgt gcagatgacg attgccatta tgccaagcaa atgcctgtaa
     7261 agctaaggct aattcagcat ctcctgtata gtctgttaag gtttctagcc aagagttact
     7321 aggctgataa cggttagcat cccacagata atggctcatg tctgtcaagg tcagcttaga
     7381 taattcccaa ctaatcatag ggttagaaac aatccccttg acctgatagt ctgattggca
     7441 taaaaatggt gagcgattag caaaaggggt taaaaagaga cgttctggat ctttttgata
     7501 atcattaaca ggaatattgt cccaaataat gataggccgc tgatatactg ctgccatggt
     7561 ttcgatatct gcttgcgaga tttgactcgc tagcgtgctt gggcctgtcc agaaaaaggc
     7621 gacttcttta ggaatccttt ctgacagctc ttgcaaatag atagaatcat ggtggttgtc
     7681 gtattctgtc ggacagatca ccaattctgg cgccgcatgt tgttggttta aaaagtgatg
     7741 aacctcagtt gccaagtggg cctgggcata ggcagtcttt ttgaatcgtc tctcaacagc
     7801 atctacaatt tggtagtcaa tatcatccag caaaagccca aaatgacaaa cacctaacgc
     7861 tagaagttgt tgcaattttt gatagagcaa ctgatagtca gcttccttgg tataatcaaa
     7921 atcaagtccc ggactaatca tataccaaaa atcaatccct tcttccttgg ctactgcaag
     7981 tagctctttg aaataagtca cccaatcctc aggataaaga tcgcgccaca gttttcgctg
     8041 gtaatcatca tctttcggag catacatgta agtattcatc cttttacgtc caatgaaacg
     8101 caggcaatct aaccgctctt ctcgtgtcca cggtgttccg taaaagcctt caattatccc
     8161 ccgcatagca aaagaaggac tatgcttgac agatacaatc ggtaaataaa gtttatcccc
     8221 tttgaaagtc atcactttaa gcaaagcttc ttgggcgtaa cgaaaacctc taaggttttg
     8281 gctttcaata ataatttcct tatcttctga aaccttcagg gtaaaggcat ccttatccag
     8341 ctgacggtta aagaaatgtt taacggtcaa ctctgcttgg ccttcttcaa tccactgaaa
     8401 atactttgac gttgccaatt ctgccagtag ggctttttga ttttcaaaac ggaaaaaacg
     8461 gccaataaag cggcatgtca acggcatgtc aaagacaaga tagtctcctc ttgtcatgac
     8521 ttcttgataa tcctttaagg cgtgataaat ggtcatttct ggtctccttt acctgagcta
     8581 accagctcat gctagtaacg tcttttttag tgactacttt ctctgataaa tacttgaaca
     8641 ggtaactggc aagacaccac tgcttggtct ggctcatgaa taatctgcaa caagacttta
     8701 gctgcttctt gccccatttg gtaaaaatct tgagctgctg tgcttaattt gggactcaac
     8761 aaacgggagg cttgactatt atcaaagcct acaatagcga cttgatcagg aatatataag
     8821 cctacctgtg tgattttttg aatcagctga atagccaacc aatcgttttc cacgacaagc
     8881 ccccttttag tggctgtcgt ctccgataag gttttcacca gacggtccaa ataagcatct
     8941 gttgtttcag tctctgtctt aggaaaataa tgggatttta gagacgattc tgccattgcc
     9001 gctaagtaac ccaaataacg gtctctaacg gatgatactt ctccaaaaga gcctgttgcc
     9061 acaaaactaa tctggtcaca gccttgctca atcaagtgct gagtcaattg acaggcaccg
     9121 cctttattat ccgctacaac agacggaaac tgaaaaagat ctagagattt atccaacaag
     9181 actagaggaa tgtgatgatg gtcgcagtaa aacaggaaat caatactgtg attgacatct
     9241 tcaggataat aaataatccc agcatagtct gacagggtgt ctaaccttac tgtactggcc
     9301 aattgaacca tgagacggta acctgtttca gcaatggagg tcataattcc cttagcataa
     9361 tctcccaatt cataatcaga agtaaaagga agaatcaaca ataaatcctt attcgcttca
     9421 ggggacgtca cttgcttttc agctacgaag cttcctttcc cacgacttct cgtaatcaag
     9481 ccttcttgct ccagttctac aagcgctcgc ttagaagtaa tacggctaac cgagaactgt
     9541 tctgacaatt ctttttcagt aggtaggcgg tcaccaattt gcaaacgacc tgatgctatc
     9601 tgctctttaa tgaaatgata tatctttttg tataataatt ttgtcattct ttactcccgt
     9661 tatcagtata gcatgtcaaa aaagagttgt aaaccttttc aaaaacttat agacaaagtt
     9721 taacttatca tttataatga aaccgaagat aaatgatata tcattttgga ggatatgata
     9781 tgacacgacc aatcccaact agcgtcagcc aatttatggc caaggtggaa agcctttgtg
     9841 gagaccaaca ccctgattgg gctcttaatt tcaaaaccag ctttaccaat accttagaga
     9901 caaccctaaa aacctacgaa gatggcacta gctttctctt gacaggagat attcctgcca
     9961 tgtggttacg tgattccact gcgcaaatga aaccttacct cttcctcgcc aaggaagatg
    10021 aagaaattag aaagattatt gccggtttgg tcaaacgcca attccgctac atttgtatcg
    10081 atccctatgc caatgccttc aacgaagaag ctaacggaaa aggacaccaa acagatcgta
    10141 cccaaatgaa cccttggatt tgggaacgca aatatgagat tgattccctc tgctacccta
    10201 ttcagctagc ttacttgctc tatcgtgaaa caggaagcac cgatcagttt aatgacgatt
    10261 ttcatcgtgg ggtagaactc attcttgacc tatggacagt tgagcaagac cacgcccagt
    10321 ctccttactt gtttgaacgc gatacttggc gcaaagaaga taccctaaca catgctggaa
    10381 aaggaagtcc tgtcgcacca acagggatga cctggtcagg ctttagacca agtgatgatg
    10441 cttgtcaata tggttacctc attccttcaa atatgtttgc agtggttgtg ctttcctatc
    10501 tagaagattt atataacaac ctttttcaca atgaaccagt agctactcgt gccaagcagc
    10561 tgaaagaagc tattcaatcg ggtattgcag accatgccct tgttcaaaac agcaagggag
    10621 aaaccattta cgcttatgaa gtggatggtc tgggacagtt ttccatcatg gatgatgcta
    10681 atattccaag cctccttgct gcaccttacc ttggtttttg cgctaaagat gaccctatct
    10741 atctggcaac gcgccgcacc attctcagcc aagaaaatcc ttattattat cagggaaatg
    10801 cagcagcagg catcggttct tcccatacac cagaaaatta tatctggcat atcgcccttg
    10861 ccttacaagg attgaccgct cttgaccaag acagtaaaaa agagatgctt gatttgcttg
    10921 tggcaacaga tgctggtacc cacttaatgc acgagggttt cgatgtcaat gatccttatc
    10981 aatacactcg tgagtggttc tcttgggcta acatgatgtt ttgtgaacta cttcttgact
    11041 atcttggctt tagcattact ccttagaaag tgaggccact atggcaacta aaaaagtaca
    11101 tattatttca cacagtcact gggatcgcga gtggtacatg gcttacgaac aacaccacat
    11161 gcgtctgatt aacttaatag atgacctgtt agaagttttt caaacggatc ctgattttca
    11221 tagttttcat ttggatgggc aaaccattat cctagatgat tatttgcaag tacgccccga
    11281 acgagaacct gagattagac aagccattgc ttcgggaaaa ctccgtatcg gacctttcta
    11341 tatcttacag gacgattttt tgaccagcag tgaatccaat gtgcgcaata tgctgattgg
    11401 taaggaagat tgtgacagat ggggcgctag tgtgccactt ggttattttc ctgatacctt
    11461 tggaaatatg ggacaaacac cacagctgat gttaaaagcc ggcctacaag ctgctgcctt
    11521 tggtcgtggc attcgtccaa ctggatttaa caatcaagta gataccagtg aaaaatacag
    11581 ctcacaattc tctgaaatca gttggcaagg cccagataac agtcgtattc ttggactcct
    11641 cttcgccaac tggtacagca atggcaatga gatcccgaca acagaagctg aggcgcgtct
    11701 tttttgggat aaaaaacttg ctgacgccga acgctttgcc tcaaccaagc accttctgat
    11761 gatgaacggg tgtgatcatc aacccgtaca acttgatgtc accaaggcaa tcgccttagc
    11821 caaccaactc tatcctgatt acgaatttgt gcattcctgc tttgaagatt acttgtctga
    11881 tctcgcaaat gatttaccag ataacctttc aaccgtccaa ggagagatta ccagtcaaga
    11941 gacagatggc tggtataccc tagctaacac ggcttctgct cgcatttacc tcaaacaggc
    12001 taataccaga gtctctcgtc aactcgaaaa catcaccgaa cccttagcag caatggctta
    12061 tgaggtgaca agtacctacc ctcacgacca actgcgttac gcttggaaaa ccctcacgca
    12121 aaatcaccct catgattcta tctgtggttg tagtgttgat agtgttcatc gggaaatgat
    12181 aaaccgtttt gaaaaagcct atgaagtcgg acactattta gcaaaagaag ctgcccagca
    12241 aattgctggc gcagttaaca cgagtgactt tccaacagat agccaacctt tcctcttatt
    12301 taataccagt ggccattcca aaacaagtgt tactgagctc accctgacct ggaaaaaata
    12361 ttattttggc caacgctttc ctaaagaggt ttaccaagaa gctcaagagt atttggcagg
    12421 actctcccaa tctttccaag tcattgatgt atcaggaaac actatctcag aagcagacat
    12481 tttggacaca agcatcgcct ttgactacga tttgcccaag agaaccttcc gagaacctta
    12541 tttcgccatc aaagtgagat tacggctacc aataactctc cctgccatgt cttggaaagc
    12601 cttagcatta caactaggaa acgagacagc tccttcagca acggtcccac tctacgacga
    12661 tagtaatcag tgccttgaaa atgagtttct aaaagttatg atacaaaccg atggccgtct
    12721 aaccatcacc gataaacagt caggggtgag ctatcaagat ctcctacggt ttgaagattg
    12781 tggcgatatt ggaaatgaat acatttcccg ccaaccaact catgaccaac ctttctatgc
    12841 ggaccaaggg accaccaagc ttaacatcat tagcaacacc gctcaagttg ctgaacttga
    12901 aatccagcaa acctttgcca ttcctatctc cgcagataag ctcttacagg ctgagatgga
    12961 agctgtcatt gacatcacag aacgccaagc aagacgttca caagaaaagg ctgagctaac
    13021 cttaacaacc cttatccgta tggagaaaaa caatcctcgc ctccaattta ccacacgttt
    13081 tgataaccaa atgacaaatc atcgcttgcg cgtcctattc ccaacgcacc ttaaaacaga
    13141 ccatcatcta gctgacagta tttttgaaac tgtcaaacgt ccaaatcatc cagatgccac
    13201 cttttggaag aatccaagta acccacagca ccaagaatgc tttgtgagtc tctttgatgg
    13261 tgaaaatgga gtcactattg gtaactatgg cctcaacgaa tatgagatct taccagatac
    13321 caacaccatt gccatcactc tcttacgttc tgttggtgaa atgggcgact gggggtattt
    13381 ccctactcct gaagcccaat gcctcggcaa acacagccta tcttatagtt ttgaaagcat
    13441 cactaagcaa acacaatttg ccagctattg gcgggcacaa gaaggccaag ttcctatcct
    13501 agtcgaacaa accagccatc ataagggaag cttaagagca gagcatagct atgtcactgg
    13561 ggcagacgat caagtcgctc taacggcttt caaacgtcgc ttagcagaca atgcccttat
    13621 cacgcgcagc tacaatcttt caaacgataa aacttgtgac tttagcctaa gcctgccaaa
    13681 ctacaatgct aaggtcacta atttgttaga aaaagacagc aagcaaagca cacccagcca
    13741 acttggcaaa gctgaaattt taactctagc ttggaagaaa caataaacta ttatcattag
    13801 taatagatta acatattcaa aaagagtctg ggactattgt ctcagactct taagttgatt
    13861 ttgagtgatt attaatacct ttaacatcag tcaggcttag ctatttctat taactgggtt
    13921 aaaatgccat catgaatctg cgagctaatt gtgagatagt cataacgatt taaaatggtc
    13981 gtgacattcc ttaagtcaaa ttgccagtca tctctttttt tcaaagccgg tgtagcaaac
    14041 acgctttgac tatcattttg tccttgttta gtactgcttt gaatgactat gacaagttta
    14101 tggttttgat ccagaaatgc tattgataag caaggttgct ttgtctccaa actcatttct
    14161 atagcattat ctaacaaaaa tgacaatagc ttagtgaaat ctactacttc tagaaaaaac
    14221 gttgcaatac aattaggaat cgatacctca accttaagct tcttattttt tgcctcaatt
    14281 aatttagcta aaacaatatg tctgattgct tcaacttgta atttatctaa acaagttaaa
    14341 tcaagtctgt catctttagc attattcttt tcagcattca agataccttc ataagtaagt
    14401 ctcacaatag agatatcatt ttgttcgatt ccaatcttaa gacgactgga cacggtagtt
    14461 aatagctgcc taagctcttg aatatcttga tacagcaatc caaggtgctg actataatta
    14521 atcagctcac gatattcttt ttgttgcaca attgcctcaa tagagttttg ttttacataa
    14581 cgctctaaat gaattaataa agtaagaaac aaaatgaaat aaaagagcac tatgattgaa
    14641 cgagcagtcg ttgtagcaag tcctgataaa gaaggcgaca tcacatctat cccttggaca
    14701 cacaagtaat atagagccat cgaagagtta gtaaacacta atagtttact agctctatgt
    14761 gattgaaaat attggcgcca ggttgaaaaa tcgaaatgaa aaacttgaat gcagcgataa
    14821 atcaaaaaaa tactaaagca agtaatgctg taaatcagta gtgtattatg tttaacaact
    14881 gaataaggca taccaaacaa tggcataaca aaaaatacaa taaatcgctt aaccaaacta
    14941 atagacacta gtggaaaaaa ggcataaaac attaaaaact tagcttttat tgatttacca
    15001 tagtataggg ctataagcgc aaagaaaaga aactgagcca acatcacaac atcaagaatg
    15061 aaatggtctg gaaagaaagc ccaaaaaact acttgaaaca aaaccatact aagtgataaa
    15121 taaaaaatat ttttcaggcg aagcggaatt gcacttacaa agctaaaaag ctttaacgtt
    15181 aacataattt caataatatc taaaaatata aatcctaaaa attgaagaaa atcttctaac
    15241 atttatcctt ctccttctcg ttcaaaatat gttatcacat ctcgattttc ttttcatttc
    15301 gaactccgta atagataaac tgagcttttt cattttaatc atattcttta ttttatcata
    15361 agttctgtca ttctaacagt taaattacga aaagcttgat taagtaactt ttctatattc
    15421 cgatcatttt actggttatc aactcagatt tatcagttgt tgatctcatt ataaacaagt
    15481 gttgcattta tctttaatat ctaacacata aagactcaca cagcctacag tttttgcaaa
    15541 ggtttcttac agcttaaaag attaaatcag gataaatcct ctatatcatc gttcatatta
    15601 gcatactaaa ataacttctg gtaatgtaac ctcctgacca ctcagttttc taatttatag
    15661 caaaaaacag ctttttttca acaaccaatt cccctaatat tgtctgtgtg tacttgctct
    15721 tcaaataaac cctacgtagt gacaaactaa caaacttttt taaccatgaa tgtttctaaa
    15781 ataaacatag agttatcaat aatatgacta tgcttagtaa accgtttttg acatccctac
    15841 tatgtcaagc tattgctcaa acccattacc tgagcttata aaacttgagt tgttattgcc
    15901 actcaatcat acacgttttt gtaacttcac gacagcctcg gtcctagctg tatgggggaa
    15961 catgtctaca ctttggatat agtgaacatc gtaaaccttt gttagctgca ccaagtcgcg
    16021 ggccaaagtg gaggtattac aagacacata caccatctgt ttaggctggt aatgaagaat
    16081 cgtttttaat aatttatcat ctaaaccagt tcttggtggg tccacaatga tggcatccgc
    16141 gcggtaacct tgtttatacc atttaggaat aatatcctct gccttccctg cctcatagta
    16201 agcgttgtca aagcccatgg ctttggcatt tttttgtgca tcttcaatcg cttctgggat
    16261 aatatccatc ccccgcacag acttgacctt ccctgcaaaa gcaaagccaa tcgagccaac
    16321 cccacaatag gcatcaataa tatgatcttt gctccctaca tccaaggcct tcaccacctc
    16381 accatacaaa acttctgtct gctgtggatt aagttggtaa aaagctctcg gtgataacgc
    16441 aaatccatag tctaaaactt cttcatgaat cgcctcttgt ccccatagaa tttccgtttc
    16501 gtccccataa atctcactcg atttagaacg attactattc aaggcaaccg ttttaacctg
    16561 aggaaatgcc ttagtcaatt cgccaatgaa attcgctaac cgaacttctt tgctactcac
    16621 aacaataatc tgtacctgat cactagcctg tgccttacga accattatcg tccgaatccc
    16681 agcaatcttg cgctcattgt aaatgggcaa cttgtacttg tccactaact gcgtaatctt
    16741 attgataata tcttgcgtta gttgatcctg taccaagcaa ttgtcaatag gaaccaagcg
    16801 atgacttccc tgggaaaata aaccagcctt gaccgtcccg ccaaatgacc gcagctgaaa
    16861 ctgcaatttg gctcgatagt gatctggctt tttcattcct aaagtcggtc taatctcaaa
    16921 ttgttcatag ccagttggtt tgaacttttt aagggcctgc ctaatcacat catctttgaa
    16981 atctagctgt ttaggatagg ctagatgcat gatttggcaa ccaccacaag tttcataaac
    17041 agaacaagct ggtttgacac gattcttaga agccttgttt accatcaaca gtttggcctc
    17101 tgcaaaattc cgtttaaccg acgtgatttg gcaaaaaata tcttctccct ttaaagcacc
    17161 agggacaaat accaaagttt tctgataaaa accaatcccc tcaccgttaa ttcccattcg
    17221 cttaattttt aaaggaattt tctgtttaac ctttacgacc atctcttccc cttacttcct
    17281 ctaatatcct aatctcacca ccaaaagcat tcacaagcaa cacattttta gcagtatttt
    17341 cgtcttctat tttaccattt ttgatataat gaacatatga aaatcactaa aattgaaaag
    17401 aaaaaacgcc tctaccttat cgaattggat aatgacgatt ccctttatgt aacagaagat
    17461 actattgttc ggtttatgtt gagtaaagat aaagtccttg acaatgatca gcttgaagac
    17521 atgaaacatt ttgcccaact gtcctacggc aaaaatttag ccctttattt tctttccttt
    17581 caacaacgca gcaacaagca agttgctgat tacctgcgca agcatgagat tgaagaacac
    17641 attattgctg acatcatcac tcaactccaa gaagaacaat ggatagacga caccaaattg
    17701 gctgatacct acattcgcca aaatcagtta aatggtgata aaggtcccca agtcttaaaa
    17761 caaaagttat tacaaaaagg cattgcaagt catgacattg atcctatctt atctcaaact
    17821 gactttagcc aactcgctca aaaagtaagc caaaaactct ttgacaaata tcaagaaaaa
    17881 ttgccaccaa aagccttgaa agataaaatc acccaagcat tactgaccaa aggcttttca
    17941 tacgatctag ctaaacatag cctcaatcac cttaattttg accaagataa tcaagaaata
    18001 gaagatcttc ttgacaaaga attagacaaa caatatcgta aactcagtcg caaatatgat
    18061 ggttatactt taaagcaaaa gctctatcag gctctctacc gaaaaggcta caacagcgac
    18121 gatattaatt gcaagttaag aaattattta tagactgaca aaagaagtga ttttttcaca
    18181 catttatgat agactataga ggataagttt aattgtagaa agttggtaag gcatgaaatt
    18241 acctaaagaa ggcgacttta ttacaattca aagttataag catgatggta gtttgcaccg
    18301 tacttggcgc gatactatgg tactaaaaac aactgaaaat gccctcattg gagttaatga
    18361 tcacactctt gtcacagaaa gtgacggtag acgatgggtg acccgcgaac ctgcaatcgt
    18421 ttactttcat aaaaaatact ggtttaacat tatagccatg attcgagata atggcgtctc
    18481 ttattactgt aacttagcta gtccatacat gatggacact gaggccctca aatacattga
    18541 ttacgattta gatgtcaaag tctttgcgga tggtgaaaag agattacttg atgttgatga
    18601 atacgagatt cataaaaaag agatgcagta ttcagccgat atggacttta tcctaaaaga
    18661 aaatgtcaaa atattggttg attggattaa tcatgaaaaa ggaccatttt ccaaggccta
    18721 tatcactatt tggtacaaac gttatcttga actgaagaat cgttaagagt tgtccagcac
    18781 tcccaaggag tgctcagaac gcagacaaac tcagttttag gctcaaaaag catgaacagc
    18841 tctgctgatt tgacatccaa tttcctattt ctgcgtgtgg agtgagccaa aacagatgtt
    18901 tttcgttgtg gtaaaagtgg cgtcaatgtt aatgttgacg ccacttggca tttttgagcc
    18961 taggatctca aaaataaggc atggaattcc aaagaaagtc gctgccttaa gcactaccta
    19021 aggaaagtat caaaaaatac taagtttagt agcgaggaaa tctccgacga aagagagtac
    19081 tcactacttt ttctttgcgt taaagtagaa gtgtcttgtt aagtcgcaaa gtcttttggc
    19141 acttgatgtt gtaaccaaaa ctgacaagac acctctttta ggtagaattt tatcaaagta
    19201 tgtaggacgc ttgacgtcgt gtattgaaaa tgaaaccact aaaataagga atcattcaag
    19261 acaaaacgat accatcggcc tcaatcatct tcttggaaac ttgaaaaccg tccataaccc
    19321 tgagattatc cttaaaacga ttaatgtcta ttctcgtcgt cttcaggttt tttcgcagat
    19381 aatggctacg cttatactcg gtttaatcct ttagaagcta agaagcaatt gaatagcctg
    19441 cacgttcgta aaacagacaa aattgacact gagaagaagt ctaataaaat ctgacagaag
    19501 atattgtggg agttaagaat cgtctgcata agcttttcca agtcaccttt tgtcttacta
    19561 taactgctaa aattaccatt aaaaccacat taagatctcc ataccatcca ttactatcca
    19621 ttaattaaac aatttcttct ataatgggat ttgtaatatg tgcatacaac ctacaacaac
    19681 acatgacttg agaacattgt aaagtgtctt ttaaacctca atctgcaatt aaaaagaaac
    19741 aataaaaagc ttaaatttgt tgtataacaa atttaagctt tttatcatta aaatacaatc
    19801 aaccaagtta gttcgattac tttaaccaaa gttactaaat agctatagtc cgtacgggat
    19861 tcgaacccgt gttaccgccg tgaaaaggcg gtgtcttaac cccttgacca acggaccata
    19921 ataatataat tatagataat gggcacgagt ggactcgaac caccgacctc acgcttatca
    19981 ggcgtgcgct ctaaccacct gagctacgcg cccaagctaa tagcttggaa aaattattac
    20041 tattgg
//
LOCUS       DAUVUY010000029        19504 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.029, whole genome shotgun sequence.
ACCESSION   DAUVUY010000029 DAUVUY010000000
VERSION     DAUVUY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19504
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.029"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(62..904)
                     /locus_tag="VOX58_000889"
     CDS             complement(62..904)
                     /locus_tag="VOX58_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HES5878128.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVSVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKADWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMAFVLFALGIWLGLWMSQLE
                     EKYK"
     gene            1307..1351
                     /locus_tag="VOX58_000890"
                     /pseudo
     CDS             1307..1351
                     /locus_tag="VOX58_000890"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1358..1639
                     /locus_tag="VOX58_000891"
     CDS             1358..1639
                     /locus_tag="VOX58_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HES5878129.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRIALRIERFKKTYDVQTYR"
     gene            complement(2115..2717)
                     /locus_tag="VOX58_000892"
     CDS             complement(2115..2717)
                     /locus_tag="VOX58_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878130.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(2714..3466)
                     /locus_tag="VOX58_000893"
     CDS             complement(2714..3466)
                     /locus_tag="VOX58_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HES5878131.1"
                     /translation="MAIPDLDKFSSNLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            complement(3532..4455)
                     /locus_tag="VOX58_000894"
     CDS             complement(3532..4455)
                     /locus_tag="VOX58_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5878132.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKHNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDENLYSFMS
                     DDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKDDIDVWVGGKSYNPARS
                     FRNNPINELTVIDFEEMADILLLMLRDNLIRSFEINVCNTLFELDKNGEPTTFNLDKI
                     DSIINKWWSENVSTEIIPNLIKKLRENPLFNIGFMVNDILERMYKEDIPKSYLTSVPL
                     VISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            complement(4506..4844)
                     /gene="cadX"
                     /locus_tag="VOX58_000895"
     CDS             complement(4506..4844)
                     /gene="cadX"
                     /locus_tag="VOX58_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HES5878133.1"
                     /translation="MKKDSICQVGVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            complement(4856..5470)
                     /locus_tag="VOX58_000896"
     CDS             complement(4856..5470)
                     /locus_tag="VOX58_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HES5878134.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLSK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            complement(5763..5912)
                     /gene="rpmG"
                     /locus_tag="VOX58_000897"
     CDS             complement(5763..5912)
                     /gene="rpmG"
                     /locus_tag="VOX58_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HES5878135.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            complement(5928..6110)
                     /gene="rpmF"
                     /locus_tag="VOX58_000898"
     CDS             complement(5928..6110)
                     /gene="rpmF"
                     /locus_tag="VOX58_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HES5878136.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            6330..7610
                     /locus_tag="VOX58_000899"
     CDS             6330..7610
                     /locus_tag="VOX58_000899"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HES5878137.1"
                     /translation="MKLQKPKGTQDILPEDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRSAYRQTLIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDVYLA
                     VLGDSANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFANISEQLLS"
     gene            7948..9696
                     /gene="aspS"
                     /locus_tag="VOX58_000900"
     CDS             7948..9696
                     /gene="aspS"
                     /locus_tag="VOX58_000900"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HES5878138.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVNGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLIAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKGNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            9689..10630
                     /locus_tag="VOX58_000901"
     CDS             9689..10630
                     /locus_tag="VOX58_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HES5878139.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIMPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            10650..11528
                     /locus_tag="VOX58_000902"
     CDS             10650..11528
                     /locus_tag="VOX58_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HES5878140.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGVLSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGFVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     EELGRGVTYLTGEGAYSGRRKKVIYVALGPRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            11571..12443
                     /locus_tag="VOX58_000903"
     CDS             11571..12443
                     /locus_tag="VOX58_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HES5878141.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(12470..12778)
                     /locus_tag="VOX58_000904"
     CDS             complement(12470..12778)
                     /locus_tag="VOX58_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HES5878142.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(12866..14557)
                     /locus_tag="VOX58_000905"
     CDS             complement(12866..14557)
                     /locus_tag="VOX58_000905"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HES5878143.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKTKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLAHIFAKMGYKPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAETDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHSVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            14848..15285
                     /gene="argR"
                     /locus_tag="VOX58_000906"
     CDS             14848..15285
                     /gene="argR"
                     /locus_tag="VOX58_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HES5878144.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            15282..15634
                     /locus_tag="VOX58_000907"
                     /pseudo
     CDS             15282..15634
                     /locus_tag="VOX58_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623648.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            15621..18176
                     /gene="mutS"
                     /locus_tag="VOX58_000908"
     CDS             15621..18176
                     /gene="mutS"
                     /locus_tag="VOX58_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HES5878145.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFDSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDHYRKVMREGTGWIADIEAKERQASGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKILATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIISVPS
                     QVESSSAVRQGQLSLFGDEEKAHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(18573..19226)
                     /locus_tag="VOX58_000909"
     CDS             complement(18573..19226)
                     /locus_tag="VOX58_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878146.1"
                     /translation="MWSSIFNFKENIVKKKLVMMLTLLISICAISVRANESGDLFTTI
                     GQSHLPGVTVAPFKTGDTTINVNLDPYGYVYGFVTSSNGNKIKKTENKTYEIPCSILE
                     KQDNPNCEGRKWTVSGNRADGGGNYTVQLEKPLAEGDKVTLSFADDGNLYFGQLVFED
                     GKTHNERLQKERDAEEQQYAEVLFKRSIEEESKKTWHQRLSDSIQDQWWNFKDWLRG"
     gene            complement(19391..>19504)
                     /locus_tag="VOX58_000910"
     CDS             complement(19391..>19504)
                     /locus_tag="VOX58_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HES5878147.1"
                     /translation="MEELNRSQSWYYESKKRALLEFVEFYRDGALKNNVRL"
BASE COUNT         5972 a         3373 c         3972 g         6187 t
ORIGIN      
        1 tctaagggga gatagagcac tagtttgttg gcgtcaactt tttgtcaaca aactgtaaag
       61 gttatttgta tttttcttct aattgactca tccaaagtcc gagccaaata ccaagagcaa
      121 ataacacaaa ggccataagc caggttaaga tacctgcatg actgtaggaa atggattcgg
      181 gactactact atttggaata agaattaata gcgtgctgga taatacaata ccaatgataa
      241 agtggtaaac ttttgaatga tggtgctgta aagcataatc catactttta gaaaaagcca
      301 agatggctag gactcctccg atagcaatag gtaagaaagt ccctaataaa tccaaagatt
      361 tgaaccctat taacatagga ccatagagtc ctaagataag taagagattt gacgggctta
      421 gtccagggac cagtactcct aaagcaatta aggcacctgc taaaataaag gttaaaaagt
      481 tagctggtaa ggtaccgatt aagtcattta agaagtaaag acctagtcct gaaataacaa
      541 aggtaaggac tagccatagc cagtcagctt tgtctctttg agactgcttg gttgactcct
      601 taatcagact aggaatagtg ccgacaatag cgccagcaaa tccccataag acgctaactt
      661 gatagtgctt aagcaaaaac tcaacgggga aagaaaagag agcaatacct aaaataccac
      721 caattcctac tggtaagaaa aataagacat tttcaataaa gttatccctc atatgagcta
      781 aaaaggaaat cattcgctca tagattccta aaatggctgc gagaacacca ccggatacac
      841 caggtaaaat aaaacctagt gcaataatca tgcctttaaa aactcttgaa ataaatgaaa
      901 ccattgttgc ctcctgaaaa gaatgatttt attgtatcat aaatttaaag aattgctgac
      961 acaaagctgc atatccctac taaaataatt gttcctaata aataatacat tcaattttga
     1021 agatattttc cttaaacacc atgtctttgt gatcaagtca agtttgattg tcaaagttgt
     1081 aaataaaatt tacaaagact gtaaacctta cgataattat taaaaagata aacgaaaaaa
     1141 agcttaggtc aaagactttt gatagtaaac ttattgaaga aggattatag tagctattaa
     1201 aaaggagatt tattatgggc tttaaaaacc agttatctta ttattgaaaa aaattacaga
     1261 tgtcacaaga agagcttgct gaaatgatgt atgtttcaag aagactatct caaactggga
     1321 aaacggtaaa agctatccag acctgcaaaa tcttttgatg ttaagtaagt ccttacaggt
     1381 tcctttagag caactaatag cagaagatat cccaaaaatg actttaatta ttaaggatga
     1441 ggagattaaa caatgtgata ggaatacgca tattatgtta tttgggacac tcctaattgc
     1501 tatcagtatt tatcctataa tgtcttggtt aggttggtgg ggatatggtc cggttgctat
     1561 cttatggttg ataatcatgc gtatcgcctt aagaattgag cgatttaaaa agacatatga
     1621 tgttcagact taccgataac ttgaagttat agcaaagggt gagagcttag atcagattga
     1681 taagattaaa gaaaaagcaa aatatcctta tcaaaaagca tggattgtta ttgcgtacac
     1741 aggattgaca gtgttaataa ccctactctc tattttgttt tttcatttgt ttttttaaac
     1801 acgaaaaaag cctttgaaac gttcatttca taggcttttc tcatctcaat tattttactt
     1861 ctgtaaaggt taagtatttt ctattttagc tgttataaac cttattgtat caatatttat
     1921 tgatagttgc ttaataaaag gtagagtgaa tggtagagta gttattgcgt tagttcagca
     1981 atcttttcaa gataaataaa tacccacagc ttctaacttt cgttgtcgta gtaaccccaa
     2041 taggtcatga tagataagaa tataaaaaag gggctgtctg gcttttgctt aacgcaagcc
     2101 acagccccaa ggaattaacg cttttgacca ataaaccaca attggttacg aactcgaaca
     2161 ggagaagaga aataatagtc tgtgtgttgg atgacaatct cttctttcag ttgtactccc
     2221 gtatccttta ggattttttg cccctgttgg tctctaggaa cttttagaaa tacagtaatg
     2281 gcatcttttc gtatatctac gatacactta cgtacagctc ggttaaaccc gttatggatt
     2341 ggattgatag tggtaacggt ctggtgttca aagttgctta ccttctgtac tcgttcacgc
     2401 tggaaaagga agtgtcttaa tagaaaggtg cgataaatag attggcagta gttcccaagg
     2461 ctatctgtat tcaagcgttg tatcatcttt aatccagata ataaggctag aagtaaagaa
     2521 ataagagcga atccataaca tacagaacta atggtatcag taacgttttt aaccgtatcc
     2581 agattgacaa atgtgacggt agataattga actgaaacaa gattgaacag ccaaccgaac
     2641 acatagcaga ttggagcaat aagcagtaag tataatatga gattgaagct tctcatgaag
     2701 taggttttag tggtcatagg atacctcgct tagtatagta ggttggacag agtaagggta
     2761 gaacttgata ggagtgttca ttatcgacaa cttgaatgat acctgttccg tgtcctgttg
     2821 gaatgacaat cccctctgga tctaggtctg gaaataagaa ttgagtggtt ttctgattga
     2881 tatttccaat ttgcaataat acgtttagct gttccctcac tgaaatggga atagtgttat
     2941 ggtcaaaacg ctggcttccc aagaatagat ggattttagt agcatgtccc aacaaggcaa
     3001 tttgtgatag tagggagaaa aaagcttctt tgatattctt attgactccc tctgatagag
     3061 cgaggacttc atcaataaca atagttagat gggtaaattg atgattggga ttatcataca
     3121 agatagcttg tcgtttctga atgagagttg cacactggtt taattgttca ttgacttgtg
     3181 aaacgaaatc agattttgaa tggttttcta ctgggtggat aacggcaatt ttattttctc
     3241 ttgcccattg gctaggggta tcgaattttg ggtcaatgat aattaggccg gacataggtt
     3301 tcaggacact caagaagtaa gtgagagcgt acggtttccc agaacctgaa tttccagcaa
     3361 tagcccagtg actcccctta tctaaattga gttcgaagtg ctttatgaca ggaattttcc
     3421 cctgttgtaa gttagatgaa aatttatcta aatcaggaat cgccaagcgt tctgaaatcc
     3481 cctttctcca agggaagaat aatccacgtt gaatgtgtaa gtcatccgtc tctaaaggga
     3541 caaaatctgt gcaaattttg atttatctat tttttgactt ccgtttgact taatcgataa
     3601 agttgacctt gcttttttgg aaataactaa tggaacagaa gtaaggtaag actttggtat
     3661 gtcctcttta tacattcgtt ctaatatatc atttaccata aatccgatat taaacaaggg
     3721 attttctcta agttttttta tgaggttggg tattatctct gttgagacat tctcagacca
     3781 ccacttattg attattgagt caattttatc tagattaaat gttgtcggtt caccattttt
     3841 atctaattcg aacagtgtgt tgcagacatt aatttcaaac gacctaatta gattatctct
     3901 caacatcaac aaaagaatat cagccatttc ttcaaaatca ataacagtta attcatttat
     3961 tggattattc ctaaaactcc tagcggggtt ataacttttt ccaccaaccc atacgtcaat
     4021 gtcatctttc ttgtgaaaag tatccatttc agtttcagta gaaatcataa atctttttct
     4081 ttgaatattg aaatttgcaa atgtcttggc aatacttaga caacttgatt ggattgaaat
     4141 taaatcatca ctcatgaaag aatataggtt ttcatctaca gcctctaaat ctctatataa
     4201 aatagaactg aaaaaataat gaaacagatt ctcaataaac tcatctaact ctccaaaaac
     4261 gatatacgta tagctcgaag cggttagagc atttaaataa actaacatcg aactcgtaat
     4321 tggtcgctta ccactttcaa cttgactaac ttgacctgca ttgcctagtt cgctttgtgt
     4381 aatattgtgt ttttttctta aatttttgat acgagtaggt atttcttgtg aataattttc
     4441 ttcaaaaaat ttcatatata atccccctat aatcatggaa aagtagaaag tagtaaaaat
     4501 agttgttatc ctagttgatt aaaaaaatct ctgatttcct catcttttat aaaataatat
     4561 ataattttcc cctctcttct agtgtccaag atgttttgat tggctagttt acgaagatgg
     4621 tgggaggcag atgccatact gagatttaat aaacaggcta tatcgcagac acagagttct
     4681 tcgacggcaa ggagataaaa gatgatattt atctgtttat tatcggtaaa ttttgataaa
     4741 atacgaagtg atttttggac tttttccttt tcaaggtagt tcgttgcggt tgtaacattt
     4801 tgttgattta taacacctac ttgacagata ctatcttttt tcataatttt ttctcctagc
     4861 ctaacacagc ccatagcatg tcaaagctgt tgttttcaat caggatatac atccccaatc
     4921 ctaaatagac aacggcaata aaccatctgc tatatttttc caaagtttct ccaacagaag
     4981 ggacttgtgc caatttttgg gcagaaaaaa ccaagagata aatcatgact agaaaggtaa
     5041 gtaaagccac tatcaaattc gctaaattta aggtagtaaa atatgggaca aagacaccaa
     5101 tattgtcagc accacaactt gcaaaagtaa tcatagcgac tagaaaaatc aggtttttat
     5161 tatctttgct caaaccttct ttggcaatag cttctccatc agaatctcct aaaagcaaaa
     5221 ctttgaggcc taggaaaatt ggaatcaaac cgagcaaacc taaaatctct ttactaggaa
     5281 tataatctaa gacaaatgca aaaagcaaac ttagcaatat tagactaaca gagcctagaa
     5341 attgtcctaa atagatgtta atgatgtctt ttctgctttt tcttttggca aaaaataaca
     5401 ttaggataat aagtaagtct acggctgtcc cagaatacag gattattgaa gtaacaacat
     5461 tttgaatcat aaaacacctc attcaaatat atttttgaat gaattttaac attaaacttt
     5521 gtagatgtca atttcagcta caccaaaata tagataagaa gttagtgtac caaatataaa
     5581 aaagccctgc catccaaatg ataacagggc ttaacttcaa tatccaaatg atatattcat
     5641 ctaaaaaagg tagagtaaaa ggtagagttc ttattgattt atattacaat gtaatgaact
     5701 tcaaaaattc aaaaatcgct taaaatcaac gttttgacgt ctaatgacgt gtagtgaacc
     5761 ccttacttaa cctctgtaaa tgtaacgtgt ttacgtaatt ttggtgagta ttttttcaat
     5821 tgaagacggt ctggagtgtt acgtttgttt tttgaagtaa ggtacaagcg ttcaccagat
     5881 tctttatgtt caagtgtaat atttacgcgc atggtatctc ccttctatta tttagcttcg
     5941 ttagctttag cgattttacg tcctttgtag tatcctttaa gggatacacg gtgagaacgt
     6001 gagtaatctc cagtagtttc gtcgaattgt acagatggag ctgtcaattt gtagtgtgta
     6061 cgacgtttgt ttttcttcgc ttttgacgtg tgacgtgcag gtactgccat tttcttattc
     6121 ttcctttcga tgtgtaagtt ttgattttca tcaacttcac tatgataaca tatctttttt
     6181 gtaaagtaaa gttacttgac aaaacttttt atttttacaa tttcatggtg aaaatactat
     6241 caaactaaag caaatgatat atgcttgttt aaaatataag aaaaaatggt agaatgttta
     6301 aagagaaaac taatcaaaga ggtaaactta tgaaacttca aaaaccaaaa ggaacccagg
     6361 atattcttcc tgaggatgct gcgaaatggc agtatgtgga atctgttgcg cgtgatacgt
     6421 ttagccaata caattatgga gaaattcgta ctcctatgtt tgaacactac gaggtaatct
     6481 cgcggtctgt gggtgatacg actgatattg tgacgaagga aatgtatgat ttctatgata
     6541 aaggagatcg tcacattacc ttgcgaccag agggtacagc tccagtggta cgttcttatg
     6601 ttgaaaataa attgtttgcg ccagaggtcc aaaaacctgt caaactttat tatatcggtt
     6661 ccatgttccg ctacgaacgc cctcaagctg gtcgtttacg tgaatttcac caaattggag
     6721 tagaatgttt cggtgctgct aatccagcaa cagatgttga aaccattgcg atggcctatc
     6781 acctctttga aaaattgggt attaaggatg tcacccttca tttaaatagt ttggggagcc
     6841 ctgagagtcg ttcagcttat cgtcaaacct tgattgacta cttaacacca atgcgtgatc
     6901 aattatctaa agatagtcaa cgtcgcttgg atgaaaaccc actgcgcgtg cttgattcta
     6961 aagaaaaaga agataaacta gccgttgaaa aggcgccttc tattttggat tacttagatg
     7021 aggaaagtca ggcgcacttt gaggcggtca aagatatgct tgaagcactt gacattcctt
     7081 atgtgattga caccaatatg gtacgtggcc ttgattacta taaccacact atttttgaat
     7141 tcatcacgtc tgtggagggc tctgacttaa ccatctgtgc gggtggacgt tatgatagtt
     7201 tggtaggtta ttttggtgga ccagaaaccc ctggctttgg ctttggtctt ggtttagaac
     7261 gtctcttgat gattattgaa aagcagggca ttaccttgcc aattgagaca gagatggatg
     7321 tttaccttgc tgtgttaggt gatagtgcta atagcaaagc tttagaattg gttcaagcca
     7381 ttcgccgtca agggtttaca gctgaacgag attaccttgg ccgtaagatt aaggctcagt
     7441 ttaaatcagc cgataccttt aaagcaaaat tggtaatgac tttaggagaa agtgaagttg
     7501 aagcaggcaa agctgtgatt aagaataatc gtagtcgtca agaagtggaa gtcagttttg
     7561 aagatatgat gacaaacttt gcaaacatct cagagcaatt attgtcttaa tgattttatt
     7621 gtcgtcataa gcttttgttg atatgattgt ttagaagata atcactaaaa caggtgtaac
     7681 ttcccttttt agaagggagt tgcacctgtt tgttttctgt aaggggattg attagctttc
     7741 ttagtattct gtgaggacac ctgatatgtc aaaatgctga cgactcagta ttcttgcttt
     7801 atctttagaa aaactctttt taaatgggtg gggtttttaa gggctttttg ctataatagt
     7861 agggaatgaa ttaattgatt tatatagtga aaggaattgg aaacgagcag gtttcacagg
     7921 attctgtttg ttttagggaa tttatttatg aaacgtagca tgtatgcagg gcgtgttcgc
     7981 gaagaacaca ttggaacgac aatcacattg aaaggatggg tcagtcgtcg tcgtgacttg
     8041 ggtggtctca tctttattga tttacgtgac cgtgagggtg tgatgcaatt agtcattaac
     8101 cctgaggaag tttccagtga tgtgatggca actgctgaac gcctacgtag tgaatatgtg
     8161 attgaagtgg aaggttttgt tgaagctcga caacaagcca atgataagtt ggcgacagga
     8221 atggttgagt taaaagtatc tgctttgacc attttaaaca cagctaaaac gacgcctttt
     8281 gaaattaaag atgacgttga agtaagtgat gacacgcgtt tacgttaccg ttacttagat
     8341 ttacgacgtc cagaaatgtt agaaaacttc aagctacgtg caaaagtgac tcattctatc
     8401 cgtaattacc ttgatgacct tgagtttatt gatgtcgaga cacctatgtt gacaaaatca
     8461 actccagaag gtgcgcgtga ctatctggta ccaagtcgtg ttagccaagg gcatttctat
     8521 gctttgccac aaagtccgca aattaccaaa cagttattga tgaatgctgg ctttgatcgc
     8581 tattatcaaa ttgtgaaatg tttccgcgat gaagatctac gtggtgaccg tcaacctgag
     8641 tttacacaag ttgacttgga aacgtcattc ctttctgaac aagagattca agacatcgtt
     8701 gaaggcatga ttgctaaagt gatgaaagag acaaaagaaa tagatgtaac cctaccattc
     8761 ccacgtatga gctacgatgt agcaatgaat agctacggct ctgataaacc agacacacgt
     8821 tttgagatgc ttttacaaga cttgacagta actgtcaatg gaattgactt caaggttttc
     8881 tcagaagcac cagctgtcaa ggcgattgtg gtgaagggaa atgcagatcg atactctcgt
     8941 aaagatattg acaaattaac tgagtttgcc aaacaatttg gtgctaaagg ccttgcctgg
     9001 gttaaggtga cagatggtca attggcaggc ccagtagcca agttcttgat agctattgag
     9061 acagagttgt caagccaatt aaaacttgct gagaatgatt tggttttatt tgtagcggat
     9121 actcttgagg ttgctaataa tacattaggt gcgcttcgta atcgtattgc caaggacctt
     9181 gacatgattg atcagtcgca gtttaatttc ctttgggtgg ttgattggcc aatgtttgag
     9241 tggtcagaag aagaaggacg ttatatgtca gctcatcacc ccttcactct tccaacgcca
     9301 gaatctgctc atgagttaga gggtgatttg gctaaggtac gggcaattgc ttatgacatc
     9361 gttttaaatg gttacgagct tggtggtggt agcctgcgta tcaatcaaaa agagatgcaa
     9421 gagcgcatgt tcaaggctct tggctttacc gctgatgaag ctaacgacca atttggtttc
     9481 ttactagaag cgatggacta cggctttcct cctcatggtg gcttggcaat tggtcttgac
     9541 cgttttgtca tgttgcttgc tggaaaaggc aatattcgag aagttattgc cttccctaaa
     9601 aataacaaag cctctgatcc aatgacgcaa gcacctagtc ttgtttctga aaaccaatta
     9661 gaagaactca gtcttcaaat agaaagtcat gattaagaaa acaacctata agaaaaaagt
     9721 taaatatgtc attagtagag gggccaagaa agttggccta ctccacgctc taagaagtat
     9781 ttcaagagaa aaatatgcag agaagatttc ggcttctctg ctttatggca ttctctctag
     9841 tattgctgtg aattttttct tccagcctgg gcatgtttat tcaagtggag caactggtct
     9901 agcacaggtt ttttcagctc ttagtcatcg tcttttaggc tatgattttc ccatcgcctt
     9961 tgcgttttat ttgattaata ttcctttgct tattttagct tggtataaaa ttgggcatca
    10021 atttaccatt tttaccttta tcacagtcag catgagttct ttctttattc aaatcatgcc
    10081 tcaagtgacg ctgacgactg atcctcttat caatgctatt tttggtggtt tggttatggg
    10141 aatgggaatt ggtacaggtc tcaaatcacg tatctctagt ggggggactg atattgtcag
    10201 tttgaccctt agaaaacgaa caggcaagga tgtgggcagt ctctcattga tggttaatgg
    10261 tgcaatttta gcctttgcag ggattttatt tggctggcag tacgcccttt attctatggt
    10321 ctctatcttt gtatcaagtc gtgttacgga tgccattttc accaagcaaa agaaaatgca
    10381 ggcaactatt gttaccagcc atccagagcg tgtgattcat atgatccata aacgtctgca
    10441 tcgcggggtg accagtatca acgacgcaga agggacttac aagcatgaac aaaaagcagt
    10501 tttgattacc attttgacat gtgaagaata cccagaattc aaatggctga tgttaaaaac
    10561 agacccacaa gcctttgttt cagtggctga gaatgttaga attatcggtc gttttgtgga
    10621 agatgattaa gaaaaacaca ggaggtgtca tgtcaattga ccaacgttta cagttaaaga
    10681 aattttgctt tattatttta ggagctgcta tctacgcctt tgcttttgtt tatttttaca
    10741 tggctaatcg cattgcagcc aatggtttag ctggtctcac cctagttggt aaggcacttt
    10801 ttgagattga tccttctttg ataggctatt tgattaattt gcctttggtc ttgctgggag
    10861 ctagatgttt tgggaaaaga gccatggttt acacagtaac gggtgtttta tcactttact
    10921 tttttgtgtg gctgtttcaa cgctttcctt tggtcgttga cttggaccat gacaatttag
    10981 tcgtttcctt gatggctgga attattggtg gtctaggtgg cggttttgtg tttcgcaatg
    11041 gtggaaccat cggtggagca gatattattg ctaagttatt agaagacaag ttaggactac
    11101 agctgaatca agctctttta ggcattgatc tttttgtcat gattgtctct ttgacttata
    11161 tttccctgcc gcaaatgatg tatgctctga ttgctagctt tatgtatggt caaattgttc
    11221 gcttggtaca acaaggtggt tattcagccc gtggcatttt tatcgtttca gaacaagcgg
    11281 agaaaattgc ccggttcatc atggaagaat tgggaagggg agttacttat ctaacaggtg
    11341 aaggagccta ctctggtcgc cgtaaaaaag tgatttatgt ggctttgggg ccaagagata
    11401 ttagggaatt aaaagctttt ttaactcagg ttgatcctaa tgcctttatc acttattttg
    11461 atgttaatga agttaatagc ccagaatttt taactattaa aagcaaatac cataaaaaga
    11521 gaaaataaat gagcattaga gttgcctgcg aagaatagtg aggataaagg atggcacatc
    11581 acgataaatt gactaaacta ctaaaactgt ttttgattgc cctaggtgta gccatttata
    11641 ccttcggttt tgttaatttt aacatggcta atgctttggc tgaaggtggt gtggcaggaa
    11701 taaccttgat tttacacgcg cattttggga ttaatcctgc ttactcttct ctcttattta
    11761 atcttccact ttttatttta ggggcaaaga tttttgggaa gcgttctttg gctctaacca
    11821 tctatggaac agttctgatg tccgctttta tctggatgtg gcaaaaagtt cctatcgaac
    11881 ttggcttgga aaatgacatg atgttggtgg ctgtagtggc aggtctcttt tcagggattg
    11941 ggagtggcat tgtttttcgc tatggtgcaa ccacaggtgg aacggatatt attggtcgca
    12001 ttgcagaaga aaaatttgga gctaagctag gtcaaacttt gcttttggtg gatgctcttg
    12061 tattgacagc ttcgttgact tatgtggatt taaaacacat gctttatact ttggtggcaa
    12121 gctttgtttt tagccaaatg attagtgtgg ttcaaaatgg tggttacact atccgtggaa
    12181 tgattattat taccaagcat tcagaggctg ccgctcaagc tatcctaacc gaaatcaatc
    12241 gtggagtgac ttacttgaaa ggtcaaggag cttactctgg taatgattac aatatcatgt
    12301 atgtgacctt gaacccaact gaagttcggg aagtcaaacg tattttagct ggtttggatc
    12361 cagatgcctt tatctccatt attgatgtgg acgaagttat tagctctgat tttaaaattc
    12421 gccgaagaaa ttatgataaa taaaaataca ggtcaaacag cctgtatttt tattttggtc
    12481 ccccacttaa gaaactacca agcataatag ctgatatagc agttcccttg taatggctag
    12541 cttttgtttt taccaagcga ctaagatcaa atatgctttt aggagcatca aattgatgtg
    12601 ttaagatgta ttgattgata agtgagtcaa tttttgtagc taggagtgag ctcatcccct
    12661 ctttttcaag ttgactggcg agctcgaaga tcgcttgacg aaaataatca tcggcttgta
    12721 ttacttgttc gctataaact ttactaagag catctaatat ttctttttct gatggcatgg
    12781 cattctcctg taacatattg tgctattatt ataatataaa aaactaagac tgtcataaat
    12841 gtgacagcct tagtaatgtt attacttaca tttcatttgg tgcgtcaaca ccgagtaagc
    12901 gaagcgcttc tttaagaaca gtagcagttg catagcagag agctaagcgg ttatcacgtt
    12961 ctgagttgtc gtcaagaatg cgagtgtgag cataatattt gttaaagctt tgggctaagt
    13021 taatagcaaa tttagccata atagaaggct caaagttatc tgatgttcgc ttgataatgc
    13081 gtgggaaatc ttggataagt ttgataattt cccagctttc agcatcagct aaactgtaag
    13141 tggtggttgc agatggcgta aagtcagctt tacgtagaat agattggata cgagcatgag
    13201 catattgaac gtaaggtcca gtttctcctt caaaggaaac catggcttca aggtcaaagt
    13261 catacccatt catacgatct gttttgagat cgtaaaactt gatggcaccg acaccaacag
    13321 agtgtgcaac ggcttctttg tcagcaaggt ttggattttt cgcttcaatt tgagaagctg
    13381 cacggttaat ggcttcagca accgtaggtt caagaagaat aacattacct ttacgtgttg
    13441 ataatttagc accgcctttt gtgacaagtc caaaagcaac gtgtgtcata tcatctgacc
    13501 aatcgtaacc catttctttt agcactgctt ttaattgttt gaagtgagca gcttgctcat
    13561 taccaacaac ataaacggat ttagcaaagt cataagtgcg tttacggtaa agtgcggcag
    13621 ctaggtcacg ggtaatgtaa agagtagcgc catctgattt tttgatcaag gcagggtgtt
    13681 caatgccata tttttcaaga ttaacaactt gggcaccttt agattctaca agaaggtttt
    13741 tagcttctaa taagtcaaga acctcatcca ttttgtcatt atagaaggcc tcaccgttgt
    13801 aactatcgaa ggtgacatgt aattggtcat aaagtcggtt gaactcaagt aaactttcat
    13861 cacggaacca ttgccataat tcagtagctt ctttgtcgcc atcttccaat ttacggaacc
    13921 attcacgagc ttcttcatca acggtgggat cagtttcagc ttcagcattg atacgaacgt
    13981 aaagttttaa gagttcatca ataggatgtg cctgaacagc agcttcatct ccccattttt
    14041 tgtaggcaac aatgagcata ccaaattgtt taccccagtc accaaggtga ttgattttta
    14101 caggtttata gcccattttg gcaaaaatgt gagcgagact atcaccaata actgttgagc
    14161 ggaggtgacc gattgaaaat ggcttagcga tgtttgggct tgacatgtca atagcaacat
    14221 ttcgcccttg accttcgtct tgctgagcat agtctgaacc tgctgtaatc acttgttcaa
    14281 ggacctgtga ggaaatctta gttttatcaa ggaaaaagtt gatgtaaggg ccgacagcta
    14341 cgactttttc aaattggctc tcatcaatct gttcagctaa ctcgctagca atcatttgag
    14401 gggctttgcg aaggactttt gctaaactaa aggctggaaa ggcaaggtct cccatgtcag
    14461 aattttttgg ggtttcaagc aagttaaaaa tagcatcttg ttctaattct ggaacaactt
    14521 tagctatttc acttgcgatt agagttttag tatccataca gcgtctccta aaaattgttt
    14581 gtcaccctat tctaacatat ttctttaaaa taaacgagga gaggattgat aaattatact
    14641 gaaactttca aggaaatgaa ttgcttgagg ttgttgtttt tctgaaaatg ttataaaaag
    14701 agaaatgagg tttcttttca acagttttca agtgaaattc tagttgaatt tgctaaaaaa
    14761 ttgctgaaaa tagctgggcg aagatgaaat aatacacttt ttttgttata atttgagagg
    14821 ataattatac aattggggtg atatatcatg aataaaatgg agcgtcaaca gcagattaag
    14881 cgaatcattc aagcagagca tattggcaca caagaagata ttaaaaatca ccttcaaaaa
    14941 gaaggcattg tggtcaccca agcaactcta tctcgggact taagagaaat tggccttttg
    15001 aaattgagag atgaacaagg caaactctat tatagtttgt ctgaacccgt tgcaacacca
    15061 tttagtcctg aggtgcgttt ttatgttttg aaagttgatc gagcaggttt tatgctggtt
    15121 ctccatacca atttaggaga ggctgatgtt ctagctaatt tgattgacaa tgatgccatt
    15181 gaagatattc taggaaccat tgctggtgcg gataccttgc ttgtgatttg cagagacgaa
    15241 gagattgcta aacgctttga aaaagatttg gctgcaggcc tatgactagc tattaagagt
    15301 ctttgacaca attgattctt gctatccatg aattctagta tcgttgctta taaagaagtg
    15361 cagcaatagc tgtctaagct ccgtgatttt aaaaagtaag cttatcagat gaaacgctat
    15421 caacaagatg ccctgctttt caaaaaaaat agataaagaa aaggctgcga cagtatctgc
    15481 aagcagggca aaagaactag aagataggct cagtcatcag ccattaattg atgattatcg
    15541 agaaaagatg caagatgcaa gtgatgtgat tcagtatatc accaaacgta tagaagatca
    15601 gttaaacaag gagttaacaa atggcaaaaa ctaacatttc tcctggaatg caacagtatc
    15661 tggacatcaa aaaagattat ccagatgctt ttttgctttt taggatgggt gacttttatg
    15721 aattatttta cgaggacgct gtcaaagcag cacaactctt agaaattggt ttgaccagtc
    15781 gcaacaagaa tgcggaaaat ccaattccta tggcaggcgt gccacatcat tctgcccaac
    15841 aatacattga tgtgttaatt gagttgggtt acaaggttgc tgtcgcagaa caaatggaag
    15901 acccaaagca agctgttggg gtggtgaagc gtgaggtcgt tcaagtcata actcctggaa
    15961 cggttgtgga ttcagctaag ccagatagcg ccaataactt tttggtagct gttgactttg
    16021 atggttgccg ttatggattg gcttatatgg atgtgtccac aggtgaattt tgcgtgacag
    16081 atttggcgga ctttacgagt gttcgtagcg aaatccaaaa cctcaaggca aaagaagtct
    16141 tactaggttt tgatttatct gaagaagaac agacgatttt ggtcaagcag atgaatttgc
    16201 tgctttctta tgaagaaacg gtctatgaag ataaatcttt aattgacggc caattgacaa
    16261 cggtagaact gacagcggca ggaaaactct tgcaatacgt tcacaaaaca caaatgcgag
    16321 aactcagcca cttgcaagca ttggttcact atgagatcaa ggattatttg cagatgtcgt
    16381 atgccactaa gtcaagttta gatttggtag aaaatgctag gactaataaa aaacatggaa
    16441 gtctctattg gctgttagat gaaaccaaga cagctatggg gatgaggctt ttgcgctcat
    16501 ggattgatcg acctttggtt tctaaagaag ctattttaga gcgtcaagaa attattcaag
    16561 tttttctgaa tgcttttatt gagcgaaccg atttaagcaa tagtttaaaa ggtgtttacg
    16621 acatcgaacg cttatctagt cgcgtgtctt ttggtaaggc aaatccgaaa gatttacttc
    16681 aattggggca taccttagcc caagtgcctt atatcaaagc tatcttagag tcttttgaca
    16741 gtccttgtgt tgacaaactt gtcaatgata ttgacagttt gcctgagttg gaatacttga
    16801 ttagaacagc cattgatcca gatgcaccag caactattag tgaaggcagt attattcgta
    16861 ctggttttga tgagcgcttg gaccattatc gtaaagtgat gcgagaagga acaggttgga
    16921 ttgcggatat tgaggctaaa gagcgtcaag caagcggcat taataaccta aaaattgatt
    16981 acaataaaaa agatggttat tattttcacg ttacgaattc aaatcttagc ttagttcccg
    17041 agcatttttt cagaaaggca actttaaaaa attctgagcg ttatggaaca gcagaattgg
    17101 ctaagattga aggtcagatg ttagaggcta gggaagagtc atctagttta gaatacgata
    17161 tttttatgtg tattcgagct caagttgaaa cctatattaa tcgtttacag aaactggcta
    17221 aaattttggc aacggtagat gttttgcaaa gtttagcagt cgttgctgaa accaatcatt
    17281 atatccggcc gcagttcaat gataatcatg tgattacaat tcaagaaggt cgtcacgcgg
    17341 ttgttgaaaa ggttatggga gtgcaggaat acattcccaa tagtatctct tttgaccaac
    17401 agaccagtat tcagctgatt acaggtccaa atatgagtgg taagtcgact tatatgagac
    17461 agctggcctt aacggttatc atggcccaga tgggttcatt tgtggctgct gaccatgttg
    17521 atttaccttt atttgatgcg atttttacgc gtattggggc tgctgatgat ttgatttctg
    17581 ggcaatcaac ctttatggtg gagatgatgg aagcaaacca agcaatcaaa cgcgcaagtg
    17641 acaactctct tattctattt gatgaactgg gacgaggtac ggcaacttat gatggtatgg
    17701 ctttagccca ggcaattatt gaatatatcc atgatagagt tggtgctaag accatatttg
    17761 caacgcatta tcatgaattg acagacttgt caactaagtt gacaagtcta gtcaatgttc
    17821 atgtagcaac gcttgaaaaa gatggcgatg ttaccttcct tcataagatt gctgagggac
    17881 cggcggataa atcttacggt attcatgtgg caaaaatagc aggactgcca aaatccctat
    17941 taaagagagc agacgaagtt ctgacccgtt tagaaacaca gtcacgatct actgagataa
    18001 tatcagtccc ttcacaagtt gagtcaagca gcgctgttag acaggggcaa ttatcccttt
    18061 ttggtgatga agagaaagct catgagatta ggcaagcact ggaagctatt gatgtcatga
    18121 acatgacccc gcttcaagca atgacaaccc tttacgaatt gaaaaagttg ttatagtctt
    18181 tcagctgaaa atgaaaaaga tgctttctat attgaaggca tctttttgtt ctgtcaaaaa
    18241 cggtccgagg ccttcggcta tttattaagt gtgttataat agtccataag aatgcgagga
    18301 aattatgaca aacattattg gacttggatt ggcagtcttt acagggtatt gacagtcctt
    18361 aagcattgtt atgactgtgt ttttgaaaaa atataggttt taagtttcct tagacttccc
    18421 tcaaaagtcc acaaaaaggt gaacaaaaaa agacctttac aggtccttta cacaatgagt
    18481 tcagcaggca agaactagcg tggtttaaat actacgcttt ttagtttgcc ctatggctta
    18541 ttatagcacg attagtgtca ttgaggaaaa aactaccctc tcaaccagtc tttaaagttc
    18601 caccattggt cttggatgct atcgctcaag cgttgatgcc aagttttttt ggattcttct
    18661 tcgatagaac gcttgaataa cacctccgca tattgttgct cttcggcatc tctctctttt
    18721 tgaagacgtt cattatgtgt ttttccatcc tcaaatacaa gttgcccaaa gtatagattc
    18781 ccgtcatccg caaaagataa ggtaactttg tctccttcag caagtggttt ttccaattga
    18841 actgtgtagt tgcctcctcc atcagcacga ttgccactta ctgtccattt ccttccttcg
    18901 cagtttggat tatcttgttt ctccaaaata gagcaaggaa tttcataagt tttgttttcg
    18961 gtttttttaa ttttattacc attacttgaa gttacaaaac cataaacata cccataggga
    19021 tctagattaa cattaatggt tgtatctcct gttttaaatg gtgctacggt tactccaggt
    19081 aagtgcgatt gccctatagt tgtaaacaaa tcgccacttt cattagctct gacacttata
    19141 gcacatattg atattaacaa agttaacatc ataactaatt tctttttcac tatattctcc
    19201 ttaaaattaa atatcgaact ccacaatacc agttaatcag tcaaaaaatc cattctactt
    19261 tttgatattt tatgtctaat ttgacccgct aagtcagatt tgcaaacgat accaagtgat
    19321 tttcttctaa tcattatttt ttagatggat atgttaattg tattagagac gtattaatct
    19381 taaaccattg tcataagcga acattatttt ttagcgcccc gtctctgtaa aattccacaa
    19441 actcaagcaa ggctcttttc ttagattcat aataccagct ttgacttctg ttaagttctt
    19501 ccat
//
LOCUS       DAUVUY010000030        18646 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.030, whole genome shotgun sequence.
ACCESSION   DAUVUY010000030 DAUVUY010000000
VERSION     DAUVUY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18646
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.030"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1022
                     /locus_tag="VOX58_000911"
     CDS             <1..1022
                     /locus_tag="VOX58_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HES5878148.1"
                     /translation="KTSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGI
                     CLLLAYLTFFILITWIISIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQY
                     IGGDGKLTETIANYSQQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKE
                     DLCRQGNTLVDTYTGKYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILR
                     LPFAGTIGVLVAFTALIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNF
                     IYPKVVGGSIGLPAMWVLMAITIGASLKGIVGMIIAVPLAATLYQVIKDNIQKRQAIQ
                     KKQVS"
     gene            1071..1751
                     /gene="radC"
                     /locus_tag="VOX58_000912"
     CDS             1071..1751
                     /gene="radC"
                     /locus_tag="VOX58_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HES5878149.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIFEEKTIFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(1753..2448)
                     /locus_tag="VOX58_000913"
     CDS             complement(1753..2448)
                     /locus_tag="VOX58_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HES5878150.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTIAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(2458..3102)
                     /locus_tag="VOX58_000914"
     CDS             complement(2458..3102)
                     /locus_tag="VOX58_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HES5878151.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
     gene            complement(3354..3701)
                     /locus_tag="VOX58_000915"
     CDS             complement(3354..3701)
                     /locus_tag="VOX58_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HES5878152.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLEKIPNSGDGFPLKITINKELTGFKLAITDQSGLRLVNIFKTKDNQILQDKFYFLMD
                     SLVEREIFSKTVV"
     gene            complement(3691..4818)
                     /locus_tag="VOX58_000916"
     CDS             complement(3691..4818)
                     /locus_tag="VOX58_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HES5878153.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            complement(4815..5795)
                     /locus_tag="VOX58_000917"
     CDS             complement(4815..5795)
                     /locus_tag="VOX58_000917"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HES5878154.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAAEVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            complement(5935..6513)
                     /locus_tag="VOX58_000918"
     CDS             complement(5935..6513)
                     /locus_tag="VOX58_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HES5878155.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFANSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHIAFKYAKSKVARCINTLKKFNDK"
     gene            6601..7272
                     /locus_tag="VOX58_000919"
     CDS             6601..7272
                     /locus_tag="VOX58_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HES5878156.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            7247..8083
                     /locus_tag="VOX58_000920"
     CDS             7247..8083
                     /locus_tag="VOX58_000920"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HES5878157.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWKRVKDAFIGEI
                     DS"
     gene            8080..8985
                     /locus_tag="VOX58_000921"
     CDS             8080..8985
                     /locus_tag="VOX58_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HES5878158.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            8989..9984
                     /gene="pta"
                     /locus_tag="VOX58_000922"
     CDS             8989..9984
                     /gene="pta"
                     /locus_tag="VOX58_000922"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HES5878159.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            10111..10866
                     /locus_tag="VOX58_000923"
     CDS             10111..10866
                     /locus_tag="VOX58_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HES5878160.1"
                     /translation="MGNIALVTGASAGFGRAIVEQLIADGIKVIGASRRLEKLKALET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGTHIRVTNIEPGLCDGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVSRD"
     gene            complement(11060..11749)
                     /locus_tag="VOX58_000924"
     CDS             complement(11060..11749)
                     /locus_tag="VOX58_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HES5878161.1"
                     /translation="MKNAISILALLTSENQLEDMFIRELSHHFSHLFEVTILTSKANI
                     ESNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTRF
                     YQKFDRHFLQYLPLQARLSDANGNIIVDNHAFNGSFLPTTDKEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            12172..12900
                     /locus_tag="VOX58_000925"
     CDS             12172..12900
                     /locus_tag="VOX58_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878162.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPEM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVANLFGGDEHA"
     gene            12893..14419
                     /locus_tag="VOX58_000926"
     CDS             12893..14419
                     /locus_tag="VOX58_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HES5878163.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            14697..15680
                     /gene="guaC"
                     /locus_tag="VOX58_000927"
     CDS             14697..15680
                     /gene="guaC"
                     /locus_tag="VOX58_000927"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HES5878164.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAERLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKACEY
                     EFVTSLKEDAPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVDGETFKEYYGSASEYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     regulatory      15803..15898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15985..16566
                     /locus_tag="VOX58_000928"
     CDS             15985..16566
                     /locus_tag="VOX58_000928"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HES5878165.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     gene            16566..17849
                     /locus_tag="VOX58_000929"
     CDS             16566..17849
                     /locus_tag="VOX58_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HES5878166.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVVGTLVSAMMGLVDTTPVVEASWIH
                     VLTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(17913..>18646)
                     /locus_tag="VOX58_000930"
     CDS             complement(17913..>18646)
                     /locus_tag="VOX58_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HES5878167.1"
                     /translation="NLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFEDARVPSHNLIS
                     QQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYLIEDGIDSALIN
                     LGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTSGIYERQFTAKG
                     KQYHHILDRQTGYPIETDMLSLTIMAPSSFYCDIWATRLFGLDSSMIITLLNTFDNIE
                     GLLVTRKHHVLMSNGLRHYFQPYYH"
BASE COUNT         5804 a         3130 c         3786 g         5926 t
ORIGIN      
        1 acaaaacaag ccttcctttt ttttatggag cagcaggtgc ctatattgtt aatattgtaa
       61 tgagcgctta cgaaaaggta tatgtttata tctttaaaga ctggtcacat gtcttaaaag
      121 taaaacgggg tatttgttta ctgttagcgt atttgacatt ttttatttta attacttgga
      181 ttatttccat tgtcattcca gacttaatta caagtattag tactctaaca aaatttgata
      241 ctataacgat acaagaagtt gttaacaatc ttgagcacaa taaactgtta gcacgtacta
      301 ttcagtatat tggtggcgat ggcaaactta cagagacgat tgctaactat agtcaacagt
      361 tattaaagca atttcttact gttttgacaa atattttaac atctgtcacg gttattgctt
      421 ctgcaattat taatcttttt attagttttg ttttttcgtt atacgtttta gcaagtaaag
      481 aagatctttg tcgtcaggga aatactttag tagatactta tactggtaag tacgctaaac
      541 gcattcatta cttgttagag ttgttgcatc agcgttttca cggatttttt gttagtcaga
      601 ccttagaagc tatgatttta ggttcgctga ctgctagtgg catgtttata ttaagattac
      661 catttgcagg gactattggc gttttagtag cttttacagc attgattcca gttataggtg
      721 cttcaattgg agcagctatt ggatttattt taattatgac tcagtccatg tcacaggcca
      781 ttatttttat cattttttta attattttgc agcagattga aggtaatttt atttatccga
      841 aagtagttgg tggatcgatt ggattaccgg ctatgtgggt attaatggca attacaatag
      901 gcgcttcttt aaagggaata gttggtatga ttattgcagt tcctttagca gcgacacttt
      961 atcaagtgat taaagataat attcaaaaaa gacaagctat tcaaaaaaaa caagtttcct
     1021 aaaaggaact tgtttttttg tcgaataaaa agatggaggt aatatgataa atgtattcta
     1081 taaaatgtga tgataataaa gccatgccaa gagaacgttt gatgcgacta ggagcagagt
     1141 ctctaagtaa tcaagaatta ttagcaattt tattacgaac aggtaataaa gaaaagcatg
     1201 tcttagagct gtcatcctat cttttatcgc atttagacag tctggcagat tttaaaaaga
     1261 tgtctttgca agaattacaa catttggcag gtataggaaa agttaaagcg attgaaatta
     1321 aagctatgat tgagttggtt tcccgaattt tagcgaccga taagacatta actgatagcg
     1381 tattaaccag tgttcaggtc gctgaaaaaa tgatggcagc tttaggagat aaaaaacaag
     1441 agcatttagt cgtattgtat ttagataatc aaaatcgtat ttttgaagaa aaaactattt
     1501 ttattgggac tgtccgacgt tcacttgcag aaccaagaga aattttatac tatgcctgta
     1561 aaaatatggc gactagtctc attgttattc ataatcatcc ttcaggaaat attgaaccta
     1621 gttctaacga ttattgcttt actgaaaaaa taaaacgatc atgtgaagat ttaggcatta
     1681 tctgtctaga tcacattatc gttagctata aagattatta tagttttcga gaaaaatcaa
     1741 ccctttttta aatcagaagt cattgacgaa taagcggaac aattgtagat cttcatctcg
     1801 aacaccttgg agaagttcag gatgccactg aacacctaaa aaaggaatgg catcgttggt
     1861 tgatatgact gcttcgatgg taccatcacg tggatctcta gcgattactt ttaaatcctt
     1921 agcaatagtt tttaaacttt ggcgatgaaa agaattaatt aatgtcttat gaccgtaaat
     1981 aggatataag atagagtctg gctcaataat catttcgtgt gataagaaat cagaaggcgc
     2041 ttcttgccaa tgtgagtcaa tgtgttggtt aagatttcct cctaaggcaa cattcattaa
     2101 ctgtgtacca cgacaaatcc ctaagattgg tttttttaat gttatagcct ccttgatgat
     2161 agctagctca aatgtgtctc gctctggtga aaaatcatca tcaaaagcag ccttctcttc
     2221 ttgataatat tttgggtcaa cgttttgccc accaattaaa atgattttat caaccataga
     2281 tacataagtt ttagcggcgg cttcatcacc aatgggtaaa agaagcggca gccccccaga
     2341 ttgagtgact gcctgtacaa atccagtagg tgcatatgac catgggagat tatccaaagc
     2401 catatttagt ctttgatttg cagttattcc aataatcggt ttggtcatta tacctcctta
     2461 atctagatga ttttgattca taaaatagag tagtgtctgg agctcactgg tcaaatcgac
     2521 agattgaaca ataactcctt taggaacttg taggtgaaca ggagcaaaac tcaagattcc
     2581 tttgattcct gcttcaataa gttgatcagt aacttcttga gcatgaatgc tgggaaccgt
     2641 taagatagcc gtttcaatgt cagtgtttgc aatccgttct ttgacggatg agataccatg
     2701 gactggaata ttatcagctg ttttagtacc aacaagagcg ttatcatcgg tatcaaaacc
     2761 catggcaatt tgcatcttat tacgatcatg gaagcggtaa tgtaatagag cacgccctat
     2821 attaccgcat ccaactaaga taacgtttgt tgtagagtga tcattaagca agtcagcaaa
     2881 aaaattcatc aatttggtaa catcatagcc aaacccacga cgccccaatt ctccaaaata
     2941 agaaaagtca cggcgtacag tagcagagtc aattcccatt gcatctgcaa tttgtttaga
     3001 actggctttt tcaacttggt cagcatggaa tcgtttaaaa atacggtaat aaagtgataa
     3061 gcgctttgca gtcgcttttg gaatagattt gtcaataacc actattttac tccttgtaga
     3121 tttctcgtta aatatgtctg tctttattgt aacaaaaaga ttgtgaaaaa tgcaacattt
     3181 tgattgtttt tgtatgagtt aaaaaagcta aaattagttt tagcactata taatagtatt
     3241 cattaggaaa gtaagttatg acagtcaaaa tgaacaacat gaattaatag caatagaagc
     3301 gaaatcattt attgacattg tgatatgact aaaaaaacaa aatttaaagt tgattagacg
     3361 actgttttgc taaaaatttc acgttctaca aggctgtcca tcaagaaata aaatttatct
     3421 tgaaggattt gattatcttt tgttttaaaa atattaacta atctaagacc agattgatcg
     3481 gtgatagcta atttaaaacc tgtgagttct ttattaatag tgatttttaa agggaaacca
     3541 tcccctgaat taggtatttt ttcgagtaaa cgtattaatt ggtagtgccc tactttagtt
     3601 tgactaaatg tagtatctct tagggtatat tttttaatgt ttggactgat tgtataaagg
     3661 tatttacagt ctttaagctt aatttctttt tcaaaagcca tctgttcctc ctaaaatatt
     3721 ttttaatgtt tgagctaatt gattaacatc ttcaatgcta ttttgatctg aaaaactgat
     3781 acgaattgat tcttttaaac gagaagagtc atccccatag tacgcagcta aaacatgact
     3841 tggattaaca gctccagcag tacaagcaga accagttgat actgcaatgc ctgctaagtc
     3901 aagttgtgtt agtaaaatag tattttggta gcctaaaaat ccaatattga gaacatgagg
     3961 aagataatga gtaccttgat taatatagta gggtagtcct tctaggaggc taatcaagtg
     4021 atgacgtaaa ctgataatgt gatcagttga ttggtcaaga caagtcatag catcagtcaa
     4081 agcttgtgcc ataccaataa tacctaacat attttcagta cttgcgcgtc gctttccttc
     4141 ttgatcaccc ccgtgaagaa gagggtcgat tggttgtccg ttactgtata aaaagccgca
     4201 tcctttagga ccatgaaatt tgtgagcaga agcagataaa aagtcaatcc caagttcgct
     4261 aggaataatc ttgagtttgc caacagcttg aacggcatca acatgaaaag cagcttgatg
     4321 gtctttgagc agattcccaa tgtccttaat aggtaacagg tcacctgttt cgttattggc
     4381 atacattatg cttaccaaaa tggtgtcatc tctcaaggct tgttttaagt cagataaatt
     4441 tatttgtcca ttttgacaag gcaaataagt gacctcaaaa ccaaatcgct cttcgagata
     4501 ggccatggta tgaagcacag agtggtgctc aatagtagtt gtgatgagat gtttgccctt
     4561 tgcttgatga gctaaagcat agccttttat ggccatatta ttgctttcag ttccaccgga
     4621 cgttactata atttgctgtt cacttgcccc caagtttcta gcgatagctt gtcggcattc
     4681 acgaaggatt ttattggctc ggcgcccgta gaaatgaata ctagaggggt taccaaagtt
     4741 atcttgcata gctgctgtca tcgccctaat cacattagga ctaagtggtg tggtggcggc
     4801 gttatcaaag taagtcatgc gttatttttg cctttaggtt gataagaaaa aagtggactt
     4861 aagggtcttc tttcatggat acgaataata gcttctgcga ttaaatcact ggcgctaaga
     4921 taagtaacgt tttctggcac acgattttta gttttaacag aatctgtaac gataatttct
     4981 ttgattggag ctgtttctaa tacctcagca gctccaccag caaataagcc atggctagcg
     5041 accgcgtaag tatcagtagc gcctgagcgt tcaagaattt tagcagcttc tgcaaaggtt
     5101 ttcccagtat ttaaaatatc atcaattaaa atggcttttt taccagaaac atcaccaata
     5161 atataacctt gttcacgttc agaatcatct tgtgcatagt cgataatagc aatcggggaa
     5221 tcaagatatt cagccaagct tctagcacgt ttaattccag aatttttagg gctaacgaca
     5281 acaacatcag aacctgataa gcctaattta ctgtaacgtt ctgcaaaaag agggactgta
     5341 aagaggttat ccactggaat atcaaaaaaa ccttgtacct gaacagcatg taagtcaagc
     5401 gtcactacac gatcaatacc agctttagtc agcatattag ctactagttt agcagtaatc
     5461 ggttcgcggg gcttagcgac acggtcttga cgagagtaac caaagtatgg caatacaata
     5521 ttgacggtat tagcacttgc acgtttacaa gcatcaatca tgatgagtaa ttcccaaaga
     5581 ttatcattga caggaaaact agtggattga ataatataga tatcatctcc acgcactgtt
     5641 tcttcaatat tgatcataat ttctccatcg gaaaattgac gtgaagacat ttttccaaga
     5701 gggattccag cagctttagc aattttttca gcaattggaa gatttgatgt gagtgagaac
     5761 agtttgattt gcttgtcagc atatcgttca gtcatgatag atttagctcc ttaacttcaa
     5821 taaaattggg taaactgttt tattaatgag tcacattacc caaaagtgtt taattgacat
     5881 ccctatttta acagaaaaac agaaaaattt cagtttttct gtcgctttta gccattattt
     5941 atcgttgaac ttttttaagg tgttaataca ccgagctact ttgcttttag catatttgaa
     6001 agcaatgtga tgttcagata aaaaagaatc aaaatcaatc tttccttgaa gagcgtcact
     6061 tacttctaat tctaattcat agtctttggt attggcatat aggttatagt ctaaggccaa
     6121 tttacctatc ggtatcactg tctcgcgacg aacggtagtt aaattgccaa aagttaccag
     6181 tgcagatggt ttgatacctt ttgaaataat aatatcaaga gcagttgatt caggaagatt
     6241 cccatgttta atcatatctt ttgcttgttc aagaaaaaga gggacattgt actcacgatt
     6301 ccccactttt tctggcactt ttaaggttaa ttcggcgcta tttgcaaatg ttcgaatacg
     6361 taatgacatt ttgttagctt ttaaatcaaa tgcttttgta tcaatatagt aattagtttg
     6421 tgtaacaggc gtcacatgtt tcatttgaga taaaagacgg ttatactcat tttttgtaag
     6481 tagagtttta tattcgattt ctaggttagt catgggaaat tccttttaat ttgtgctata
     6541 ataaattgtt atttcaattt tatattatta tcctggcttt gacaagaaag gagacaatag
     6601 atgaccttag attgggaaga atttttagat ccttatatcc aaacagttgg tgaattaaaa
     6661 ataaaactac ggggtattcg caagcaatat cgtaagcaaa atcgttattc tccgattgaa
     6721 tttgttacgg gacgtgttaa gtcaattgaa agtattaaag aaaaaatgat tttacgtggt
     6781 gttattgaag aaaatatagc acaagatatt caagatattg ctggactacg tattatggtt
     6841 cagtttgtcg atgatgtgga agaagtctta gctctattgc gtcaacgaca agacatgaca
     6901 attgtttatg aacgagatta tattcgcaat atgaagtcca gtggctacag gtcttatcat
     6961 gttgtggttg agtaccctgt tgatactatt gaaggtcaaa aaaaggtctt agcggagatt
     7021 cagattcgta ctttggcaat gaacttttgg gcgaccattg agcattcttt aaattataaa
     7081 tatggtggag acttcccaga agaaattaaa aagcgtcttg aagtgactgc taaaattgct
     7141 ttagaattag acgaagaaat gcgtaagatt cgagaagata ttcgggaggc tcaattattg
     7201 tttgaccccg tgacacgaaa tttaagtgat ggtgtaggaa atagtgatga cacagatgaa
     7261 ttatacaggt aaggtaaagc gagttgctat tattgcaaat ggtaagtacc aaagtaaacg
     7321 cgtcgcctcc aaacttttct ccgtatttaa agatgatcct gatttctatc tttcaaagaa
     7381 aaatccggat attgtgattt ctattggcgg agatgggatg ctcttatctg cctttcacat
     7441 gtatgaaaaa gaattagata aggtacgttt tgtaggaatc cacaccggtc atcttggctt
     7501 ttataccgat tatagggatt ttgaagttga taaattgatt gataatttaa gaaaagacaa
     7561 gggagaacaa atctcttatc ctattttaaa agttgctatt actttagatg atggtcgtgt
     7621 ggttaaagcg cgtgctttga atgaagcgac ggttaagcgt attgaaaaaa cgatggtagc
     7681 agatgttatt attaaccatg tcaaatttga aagcttccga ggtgatggga tttcagtatc
     7741 gaccccgaca gggagcacag cctacaataa atctttaggt ggtgctgtct tgcatccgac
     7801 gattgaagcg ctgcaattga cggaaatttc cagtcttaat aaccgtgtct ttagaacctt
     7861 gggctcatca atcattattc ccaaaaaaga taagattgag ttagtgccaa aacgattagg
     7921 aatttatacc atttccattg ataataaaac ctatcagtta aaaaatgtga cgaaggtgga
     7981 gtattttatc gacgatgaga aaattcattt tgtttcctct ccgagtcata cgagcttttg
     8041 gaaaagggtc aaggatgcct ttattggaga gattgactca tgagatttga atttgtagca
     8101 gacaaacgaa taaaagtaaa aaccctttta aaaagtcatg atgtctctaa aggactattg
     8161 gctaaaatca aatataaggg cggtaatata ttagtgaatg gtattgaaca aaatgccatt
     8221 tatttattgc aagtgggaga tgttgtcacc attgacattc caaacgaaga gccctttgaa
     8281 aagcttgaag ccatcccttt tgatttggat attgttcacg aggatgatca ttttttggtg
     8341 attaataaac ccattggctt tgccagtatt ccaagtgcca tccactccaa taccattgct
     8401 aactttatca aggcatatta tgtggacaat cattacctag accaacaagt gcatatcgtg
     8461 actcgtttag acagagatac gagtggcttg atgttatttg ctaagcatgg ttatgctcat
     8521 gcaaggttgg acaagcaact ccaaactcgc tcaatagaga aacgttattt tgctctggtt
     8581 tctggaaatg ggacgttgcc agatgaagga gatattattg ctccaattgg gcgttcaaaa
     8641 gatagtatta ttaccagggc agttgaccct atgggcaaat atgccaagac tagctataag
     8701 gttgtggcgc gctattcaga aaatgtgcat ttagtggata tcaaattgca tacaggacgt
     8761 acccatcaaa tcagagttca ttttgcccat attggttttc cacttttagg ggatgacctt
     8821 tacggaggtc gtttggactt aggaataaca aggcaagctt tacattgtca ttatttgaat
     8881 ttcaaagatc catttacaga aagtgattgt agctatgcaa tacacttgac agatgacttt
     8941 gatagcgtta tcataggttt acagaaaaaa gatagaggtt attaaagaat gagtattcgt
     9001 agtttattcg gtggtttaag agaaaaaatt cttggtaaaa acatgaagat tgttttccca
     9061 gaaggtaatg atgagcgcgt cgtccgtgca gctgctcgtt tgaaatttga aggactctta
     9121 gaaccgatta tccttggaca gtcagaagag gtacgcaacc ttttgacaaa attaggcttc
     9181 gctgatcaag actatactat cattaatcca aatgaatatg cggattttga caaaatgaaa
     9241 gaagcttttg ttgacatccg taaaggaaaa gcaacgcttg aagatgctga taaaatgctt
     9301 cgtgatgtga attactttgg tgttatgttg gttaaaatgg gattagcaga tggaatggta
     9361 tctggtgcta ttcattcaac agctgatact gttcgtccag cccttcaaat cattaaaacc
     9421 aaaccaggta tttcaagaac ttcaggtgtt ttcttgatga atcgcgaaaa tacgagtgag
     9481 cgttacgtgt ttgctgattg tgctatcaat attgacccaa cagcacaaga attggcagaa
     9541 atcgctgtta acacagctga aacagctaaa atctttgata ttgatcctaa aattgctatg
     9601 ttaagcttct caacaaaagg ttctggtaaa gcgcctcaag ttgataaggt aagagaagca
     9661 acagaaattg ctaaagggtt gaaccctgat ttggcacttg atggtgagtt gcaatttgat
     9721 gcagcctttg ttcctgaaac ggcagctatc aaagcaccag attcagcggt tgctggccaa
     9781 gccaatactt ttattttccc tgacttacaa tctggaaata ttggttacaa aattgcacaa
     9841 cgtttaggaa tgtttgatgc tattggacca atcttacaag gattgaacaa acctgttaat
     9901 gatttatctc gtggctcaag cgctgaagat atttacaaat tagctattat tacagcagca
     9961 caagctatcg aaagccaagg gtaacttaag accccttgat ataggattct aaccgctaag
    10021 tgatgtgtta gaagttatcc taagactaat tgacattatt aaaaacgagt tggccagacc
    10081 atctcgtttt ttttgaaggt gaggaaatgt atgggaaata ttgccttagt aacaggggct
    10141 tcagccggtt ttggcagagc cattgtcgaa caattaattg cagatgggat aaaggttatt
    10201 ggtgcttctc gtcgtcttga gaaactcaag gccttagaaa cgagctttgg gtcggacaac
    10261 ttttaccctt tgcagatgga tgtcacagat accaaagcga ttgatgatgc tttgacaagt
    10321 ctaccaaatc agtggcaaga tattactatt ttagtgaata atgcaggctt ggcattggga
    10381 cttgataagg cttatgaagc cgattttgag aattggatga cgatgattaa cactaatatt
    10441 gttgggttga tttatctcac acgtcaatta ttaccacata tggtctccaa agatgatggt
    10501 attattatta atttagggtc aaccgcaggg acaattccct atccgggagc taatatttat
    10561 ggggctagca aggcttttgt taagcagttc tcactcaatt tacgagctga tttagcaggt
    10621 acccatatac gtgtgaccaa tattgaaccc ggtctatgtg acgggacaga attttcaaag
    10681 gttcgcttta atggtgatga ggcacgggtg aaggccattt atcaaggagc acatgccatt
    10741 caacctcaag atattgcgtc gactgtatca tgggtgattc atcagccccc tcatgttaat
    10801 gttaatcgta ttgagctgat gccagtgtcg caaagttatg gaccgcaacc agttagtcgt
    10861 gactagcaag ggatgagtcc ataagaataa taatctttag aaaaatggtt tactggtatg
    10921 gattattctt tataagtgag acttaccgat ttagtcatca aaaaaagcaa tcaagaagaa
    10981 ggttttgagg tcttcaagcg ttcatgattg cttcttgtgc gttgatgatt taggctgaac
    11041 tccctattta aaatcaagtt tagtgatcta aaatggaagg gcctgatgtg acatcggacc
    11101 agccaacaat ggcttgtcca gtttcttcta aatactggtt taataacggt ttaatgtctt
    11161 gaacggtatc caaaactccc accagctgac cttgaggatt tttaagagct tgataatgct
    11221 gcatgtaaat atgatccaat gaacccgaag gaagtaggaa ggttttacaa gggttatctg
    11281 ataaacgcaa ttcagcaaga atccaatctt ctatttcttt atcagtagtt ggcaaaaaac
    11341 ttccattaaa agcatgatta tcgactatga tgtttccatt tgcatcagat aaccttgctt
    11401 gcaaaggaag gtactgtaaa aaatgtctat caaatttttg gtaaaatcta gtgaggtatt
    11461 ggcttaacga taagactgct gttgcttgat caaagacact ttctgtcact aaaaatccct
    11521 gaccatgttg agaggttttg aaatagagtg taggcaagtc aatgtcatgc tcatggaaac
    11581 tgaaaattcc ttggaaagtc gacaattgat ttgactcaat gttggctttg ctagtgagaa
    11641 tagtcacctc aaaaaggtgt gagaagtggt gggaaagctc tctaataaac atatcttcaa
    11701 gttggttttc agatgttaag agcgctaaaa tggaaatggc atttttcata attgtatgat
    11761 agccgtattt tctagaaaag acaactaatc tgcttatcta tcataggcaa gtctaggatt
    11821 tttgataagg taattttgga gagataggct gacaaagtga cctgtttttc tataaggggt
    11881 aaggagaaat agtctcatta tttgacttat agtgggaaac tttttataat ggtcaacaac
    11941 aaacgacaag aaaataatga gagaaaaagt ttttgagaat tggtagtttc taccttactg
    12001 aggataccta agagtccctt taattagtag atgcgtttgt ctaaaaatgg tagggcatgc
    12061 agtgattcct tgtagttact cagaacgttt tgagaaaaaa tgacagagag acaggttgaa
    12121 tgaactcatc agcttttatg ctgttacttt aaccaatgaa ttggagaaat aatgatacgt
    12181 tttaataatg tttctaaaac ttttggtcag acaaaggttt tgcaagagca gacctttcaa
    12241 attaatgacc gtgaattttt tgtgctggtt ggtcccagcg gatcggggaa aaccacactt
    12301 ttaaaaatga ttaactgcct tattgaacca agctctggag atattttact gaataacgtc
    12361 ccacagaaag agttagattt acgtgagatg aggctatcta ttggatacgt gttgcagcaa
    12421 attgctcttt tccccaatct cacagtagct gaaaatattg cgattatccc tgagatgaag
    12481 caatggtcag ctgaagaaat tagacaaaaa actgaagaat tattggataa ggtgggccta
    12541 cctgctaaag attatcttga tcgttaccca agtgatttat cgggaggtga acagcaacgt
    12601 attggtattg tacgtgctat catttctcat cctaaaattc tattgatgga tgaaccattt
    12661 tcagccttag atcctatttc acgtaaacaa ttgcaagagt taatgttgag tcttcacaag
    12721 gaatttgaca tgacgattgt atttgtgaca catgatattg atgaggccat taaattagga
    12781 gaccgcgtag ctattttaaa tgagggagaa attgtgcaac tggatcgccc agaaatgatt
    12841 aagacccacc ctgctaatgc ttttgtggcc aatttatttg gaggtgatga gcatgcctag
    12901 tttgtttgta actttccaaa accgttttaa tgaatggctg gcggctctgg gggaacacct
    12961 gcaaatttcc cttttatctc ttatgatcgc cttgctaata ggtgtgcctt tagcagccct
    13021 tctcagtcgc agcaaacgtt ggtcagacat tatgttacag gtaacaggtg tttttcaaac
    13081 cattccctca ctggctttgc ttggtctttt catcccttta atgggaattg gaacgttgcc
    13141 tgcagtgaca gctttagtta tctatgcgat ttttccgatt ttacaaaaca ccatcacagg
    13201 attaaatggt attgacccaa gtctcgtgga agcaggaata gcttttggaa tgaccaaatg
    13261 ggagcggtta aaaacgtttg agattccaat tgcaatgcct gttattatgt caggtgtgcg
    13321 gacgtcagca gtcatgatta tcggcacagc tactttagct tccttgatag gagccggtgg
    13381 acttggctct ttcatcttat tggggattga tcgtaataat gctaacctga ttctgatagg
    13441 ggctatttct tcagctctgc tagctattat tttcaatagt ttgttacagt accttgagaa
    13501 agcttccttg cgacggatta tgattagttt tggaatcacc ttacttgcat tgctagcatc
    13561 gtatactcct atggcgctta gtcagttttc aaaaggaaaa gatacagtgg ttattgccgg
    13621 taaattggga gcagagcccg atattctgat taatctctat aaggaattaa ttgaagatca
    13681 atcagatata agtgttgagt tgaaatctaa ttttgggaaa actagcttct tatatgaagc
    13741 ccttaaatct ggagatattg atatctatcc tgaatttaca ggaaccataa catcaagtct
    13801 tttacgcgac aaaccacctt tgtctaatga ccctaagcag gtctatgagg atgctaaaaa
    13861 aggcattgct aagcaagata aactgaccct tcttaagcca tttgcttacc aaaatacgta
    13921 tgctgttgct atgccagaaa aattggctaa ggaatatcag attgaaacca tttctgattt
    13981 aaaagcgcat gctgatactt taaaggctgg ttttactctg gaatttaagg acagagcaga
    14041 tggctataag ggaatgcaat ctcaatatgg attacagcta tctgtggcga cgatggagcc
    14101 agctcttcgt tatcaagcaa ttcaatcagg agatatccaa gtaacagatg cttactctac
    14161 ggatgctgaa atcacgaaat accatttgaa agttttaaaa gatgataaac agttgtttcc
    14221 accttatcag ggagctcctt tgatgaaaac ttccttatta acaaaacatc cagaattaaa
    14281 aggcattctt aatcaattag cgggaaaaat tactgaaaaa gagatgcagg acatgaacta
    14341 cgaggtatct gttaaaggag cagatgctaa taaggtcgct cgagattatc tgttgaaaac
    14401 aggcttaatc cagaaataaa cagaaatgtt ggtccatagg actggcattt tttcgtctct
    14461 taagttcaag aagtaacaat tcataaatac aaaaaccgaa catttaaatt aaaaatagta
    14521 tttaaatttg acaaaatata aacaaataag taatctataa aatgaaaggt aagtttttac
    14581 ctaaatatac ctctaatcat cagaggtcat aggaagatta ttttgtatag aggacttaga
    14641 gaagaaggtg agaactgact tgtttttctt aatctatgtt aaaagaggag aaacttatgt
    14701 ttaatgacat ccctgtattt gattatgaag atattcaact gattcctaac aagtgcatta
    14761 ttactagtcg ttcacaagca gatacaagtg tgacactcgg aaaataccag ttcaagctac
    14821 cagttatccc agcaaatatg caaaccatta ttgatgaaac aattgctgag cggttggcaa
    14881 aagagggtta tttttatatt atgcatcgtt ttgatgaaga tagccgcaaa ccttttatca
    14941 aacgcatgca tgaacaaggc ttgattgcat caatctctgt tggggttaaa gcttgtgaat
    15001 atgaatttgt gacctctttg aaagaggatg ctcctgagtt tatcaccatt gatattgctc
    15061 atggtcatgc taacagtgtt attgatatga ttaagcatat caagactgaa ttaccagaaa
    15121 catttgtcat tgctggaaat gttggtacgc cagaagctgt acgagaactc gaaaatgcag
    15181 gtgctgatgc tactaaagta ggcatcggtc caggcaaagt atgtatcact aaagttaaaa
    15241 ccggtttcgg tacaggtggt tggcagctag cagcactgcg ctggtgcgca aaggcagctc
    15301 gtaaacctat cattgctgat ggtgggattc gcacacatgg tgatattgct aaatccatcc
    15361 gctttggagc tagtatggtc atgattggat cgctttttgc aggacatatt gaaagtcctg
    15421 gaaaaacggt agaagtggat ggtgaaacgt ttaaagaata ttatggctcg gcgtcagaat
    15481 atcaaaaagg cgagcataaa aatgtagaag gcaaaaaaat cttattgcct acaaaaggtc
    15541 acttatcaga taccctgacc gaaatgcaac aggatttgca atcgtccatt tcttacgcgg
    15601 gtggtaaaga tttagacagt ttgcgtcatg ttgattatgt tattgtcaaa aattccattt
    15661 ggaatggtga ttccatttaa atgatatgga gttagctagg accagtaaag aattttttag
    15721 cttaatgctg gagatgcttt tatcatatct ctgtaggtat tgaaaaaaat aagaaacatg
    15781 gtaaaatagt aaattatcat aatgaattca ataatgacat acttatttat gctgtgaatt
    15841 ggcgcagcgt ctctacaaga caccttaatg tctaacaata agtaagcttt taggcttgct
    15901 tggtttttaa ccttgcaaag tgagaaactc ttatagtttc tcactttgcg gcaggctttt
    15961 tctatttgaa tcaaaaggag atttatgcag ttacttgaag aacgcatctt aacagatggc
    16021 aatattctag gagagaatat tctaaaggta gataattttt taactcatca agttgattac
    16081 cggttgatga aagcaattgg taaagtgttt gctcaaaaat atgctgaggc tggcattaca
    16141 aaagtggtta caatcgaagc ttcaggtatt gcaccagccg tatacgctgc agaagcaatg
    16201 gatgttccta tgatttttgc gaaaaaacat aaaaacatta ccatgacaga aggcattttg
    16261 acagcagaag tttattcttt cactaaacaa gtgacaagca cggtgtctat cgctggtaaa
    16321 ttcctatcta aagaagacaa ggttttgatt attgatgact ttttagctaa tggtcaggca
    16381 gccaaaggct tgatcgagat tattggtcaa gcaggggcac aagtcgtcgg cgttggtatt
    16441 gtgattgaga aatctttcca agatggtcgt cgattgattg aagatatggg cattgaagtg
    16501 acctcattag cacgtattaa aaactttgaa aatggtaatc taaacttctt ggaggctgac
    16561 gcataatgac acattcaaca aagcaggaac attctcactc gcaatcagct gtccttggct
    16621 tacagcatgt gctttcgatg tatgccggat ctattttagt accaattatg atcgcaggtg
    16681 ctttaggtta ttccgctaga gagttgacct acctgatttc gacagatatt ttcatgtgtg
    16741 gtgtggctac tttcttacag ttaaagttaa ccaagcatac gggagtggga ttaccagttg
    16801 ttttaggatg tgcttttcaa tcagtggcac ctctatccat tattggagcg caacaaggtt
    16861 caggtgccat gtttggagcc ctcattgcgt caggaattta tgtcatttta gtggcaggta
    16921 ttttttctaa gattgctcgt ttcttcccac caattgttac aggatcggtc attaccgtta
    16981 ttggcttaag tctcgtaggt gttgctatgg ggaatatggg agataacgtc aaagagccta
    17041 cggcacaaag catgatgtta tcattgttga cgattgtcat tattctttta gtccaaaaat
    17101 ttaccaaagg ttttgtgaaa tccatttcca ttttgattgg gcttgtagtg ggaactttgg
    17161 tttcagccat gatgggatta gtagatacaa ctcctgttgt agaggcttct tggattcatg
    17221 tgctaacccc attttatttt ggcatgccaa cttttgaaat cacttctatt gtgatgatgt
    17281 gtatcatcgc aacggtttct atggttgaat caacaggtgt ctacttagcg ctttcagatt
    17341 tgactaatga tcaattagat gaaaaacgtt tgcgcaatgg ttatcgctca gaagggatcg
    17401 cagtctttct cggtgggtta tttaatacct tcccttacac tggtttttct caaaacgttg
    17461 gacttgtcca aatctcaggt attaaaaccc gtcgtccgat ttactacgca gcaggtatcc
    17521 ttgtggtcat tggcctactc cctaaatttg gagcaatggc acaaatgatt ccaagcccag
    17581 ttcttggtgg agctatgctt gttctctttg gtatggttgc tcttcaagga atgcaaatgc
    17641 ttaatcgtgt tgatttccaa aagaacgagt ataattttat tattgctgcc gtttccatct
    17701 cagcaggttt aggctttaat ggcactaatc tttttgccag cttaccagag acagctcaaa
    17761 tgttcttaac gaatggtatt gtgattgcca ctttgacatc tgttgtctta aatttagttt
    17821 taaatggcaa agacaaacaa gatgaataat taaaaaacgg ctaattgagc cgttttttct
    17881 gtgatcagtt ttgagcaaac cgttaacccc ttttaatgat agtatggttg aaaatagtgt
    17941 ctcaaaccat ttgacataag gacatggtgc tttcttgtta ctagaagacc ttcaatatta
    18001 tcaaaggtat ttaaaagtgt gataatcatt gagctatcaa gaccaaataa acgggtagcc
    18061 catatgtcac aataaaaaga agaaggagcc atgatcgtta agctaagcat atctgtttct
    18121 ataggatagc ctgtctgacg atctaagata tgatgatact gtttcccttt ggctgtaaac
    18181 tgcctttcat aaattccaga agtcacaaca gagtgattgt taaccttgat gacaccaaga
    18241 tgttgcccac gtttagcatc tggtttttga atgccaattc gaaaagtctt gtcagggctt
    18301 ttcggattag gtccatgaac gcgtacattt ccacctaaat taataagggc agagtcaata
    18361 ccatcttcaa tcaggtaggc cataatctta tcagtaatat aaccttttgc taaggctcct
    18421 aagtctaaag ccattcctac ttgttgtaaa aagacagtct gttttttgtc atctataaga
    18481 acctgtctag gatctgttag agctaactgt tgtgagataa ggttatgact aggcactcta
    18541 gcatcttcaa atccaattcg ccaggcttga acaagtggcc caatggcgat attaagatta
    18601 cttggtgttg acaagctatg agctttacca atttggatta ggttaa
//
LOCUS       DAUVUY010000031        17452 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.031, whole genome shotgun sequence.
ACCESSION   DAUVUY010000031 DAUVUY010000000
VERSION     DAUVUY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17452
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.031"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..725)
                     /locus_tag="VOX58_000931"
     CDS             complement(<1..725)
                     /locus_tag="VOX58_000931"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HES5878168.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMAT"
     gene            complement(718..1038)
                     /locus_tag="VOX58_000932"
                     /pseudo
     CDS             complement(718..1038)
                     /locus_tag="VOX58_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623380.1"
                     /note="catalyzes the formation of glutamate 5-phosphate
                     from glutamate in proline biosynthesis; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
     gene            complement(1065..1250)
                     /locus_tag="VOX58_000933"
     CDS             complement(1065..1250)
                     /locus_tag="VOX58_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HES5878169.1"
                     /translation="MQENKNEQNTGTKTIKKIGKVTYEVVVHFNENATETMQDKLKRI
                     MLREMEMEKHQKGDKND"
     gene            complement(1352..2341)
                     /locus_tag="VOX58_000934"
     CDS             complement(1352..2341)
                     /locus_tag="VOX58_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HES5878170.1"
                     /translation="MADENKEIVIVDDKTIQEKIYFIRGQKVMLDADLAEIYGYETKN
                     FNRQVKNNIEKFEGEDFMFQLTENEFENLRCKNFTSSWGGSRYLPNAFTEQGIYMLMT
                     VLRGELAIRQSRALIRTFKQMKDFIIENQDFIGSKELVQIAIQTNQNTNDIAEIKSQM
                     ATKEDLKKVMDNFIDPDTYKHFLLMNGDKIEADVAYTKIYKSAKKSIYVIDNYIGLKT
                     LELLRAAKDNVEIIVFSDNVKNKDMLTKNILNDFRRDYPNINLKMKVAGKKYHDRYIA
                     IDYGTENEAFYLCGASSKDAGNKISSITKIEESSKDMYHTMFGGMLNNKNLKI"
     gene            complement(2415..2738)
                     /locus_tag="VOX58_000935"
     CDS             complement(2415..2738)
                     /locus_tag="VOX58_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878171.1"
                     /translation="MRIQFTVNDEELKILTKKVIEGNYPSISEYCKCSSLQENTSYVD
                     LYNTLLNKISFLPKDKEFVLRELIATPPALIGRWFYENVNKGLVKNVEHIGKAEGGVE
                     KYKKI"
     gene            complement(2920..3666)
                     /locus_tag="VOX58_000936"
     CDS             complement(2920..3666)
                     /locus_tag="VOX58_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HES5878172.1"
                     /translation="MADNRRYYWLKLKEDFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDSGKLYYDGVETDFIKELALTIDETEDDVMVTVNYLMAQGLMEIITENDEYFLTE
                     IPSLIGSETASTRRSRKSRGQKALQCNTNATHCNLLQQNCNGDIEKEIDIELEKEGEI
                     EKISPVFYGEYKNVSLTDEEYGKLKDKMQGHREKMIEKLSTYMQSTGRNYKDHYATLI
                     HWYEQDKGKLKECSKSTVYTLEDYDKGEHL"
     gene            complement(3939..5579)
                     /locus_tag="VOX58_000937"
     CDS             complement(3939..5579)
                     /locus_tag="VOX58_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HES5878173.1"
                     /translation="MEIKSLHTHVDIVARSKGHSVIAKAAYNARDKLQDEYYGKTHDY
                     SKKEDLVFSKIFLPEHIPKEFSNREYLWNSVEKIEKSKNSQLARNLLFTLPRELNEQD
                     RIKLISEFIEENFTSKGMIADCNIHNPMASDHEEQPHAHILLTLREIDEKGNWKPKCR
                     KEYILDENGEKIKLKSGNYKSRKVNLNDWNEPDKAKEWRENFSKKANEYLARNNINKR
                     IDPRTFKEQGREELPQIHLGTSSYQMEKKGIQTERGNQNRKIIALNLEFRKLKEELSK
                     LTSWIGSLLGSLQVKYDEYKQEKKEEYENKAELFNLYEYISIYYDLQGEKARKLNPYA
                     SNKKIGADLRRFSKARIYLKDNNLKTIADLQEKISTLQSQNKKISQDIKAKTTRIENL
                     NKCFAYADIIKDNKKVFEEWNSKSLFKDSFYNSHKDEIDKYKRARAILKKITGSSAIK
                     SKDWKKEIQSLEDEISKLNRQSQSIKEEYESINHIKYAVKTVNDDYGIDLSIEIDKAI
                     KRGEKPSVIAQLRKFQEQQERKEQYKQKAKEKYTEQER"
     gene            complement(5895..6269)
                     /locus_tag="VOX58_000938"
     CDS             complement(5895..6269)
                     /locus_tag="VOX58_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878174.1"
                     /translation="MKELENVMSQLEKETKKKEQAENKIKQLRQKKSQLKRKADTKRK
                     VEKGGVFEKFEKQITGAEESTDNDLIYAFLDYVLSDNRNREKLKELTEIHLKEKEISL
                     EENPNLKEENANDDFEELTEEE"
     gene            complement(6356..6772)
                     /locus_tag="VOX58_000939"
     CDS             complement(6356..6772)
                     /locus_tag="VOX58_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HES5878175.1"
                     /translation="MDKKEYFLYVKGKAVPVSEEVYKAYWKITEHEKYLQRKDWKYGV
                     IPFSAMDYDGHFVDNIIDERIDLEKIVEVKMQIEELNKALATFTKKERELMEAIFYRE
                     ESLRSIGKKEKVSYQAIGKRRDKILEKLRKLLEDKF"
     gene            complement(7066..7418)
                     /locus_tag="VOX58_000940"
                     /pseudo
     CDS             complement(7066..7418)
                     /locus_tag="VOX58_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(7415..7633)
                     /locus_tag="VOX58_000941"
     CDS             complement(7415..7633)
                     /locus_tag="VOX58_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HES5878176.1"
                     /translation="MKKRFLKDVLVLIGIMFVIFIVCIFLPEKIPVHFNAKGIPDMFA
                     NKYYLLFAAVIPYSAYWKFVRGRKNKNK"
     gene            complement(7674..7931)
                     /locus_tag="VOX58_000942"
     CDS             complement(7674..7931)
                     /locus_tag="VOX58_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HES5878177.1"
                     /translation="MIDLVRIFDSLSNPIRMDILLIISNKNLTAGEIASHFKLEKATI
                     SYHLSLLKKSKLITDSRYKNYIYYSINRDTFVEVIRWLDKF"
     gene            complement(8027..9583)
                     /locus_tag="VOX58_000943"
     CDS             complement(8027..9583)
                     /locus_tag="VOX58_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878178.1"
                     /translation="MRKIWILIRAQLINFFPINEMKEPGNKKQSSIVIASFGIITLAI
                     FFFVYNIITAKTLVQLGQQELIPAYMVSVSSFAILFLTIFYSNGILFGSRDMENLLSL
                     PVKSSDIISSKFIFMYLLNFLIGLMFMLPGGIVWVLNGSLNVLQIILYFTSIIFVPLI
                     PMCIAACMGIIIVVASSFFKRKNVIALIFSFAMIGIIGYIAVSAMKSGNENSIGIMLS
                     KQITGLYPLSKLFMEYYNFPMYIGVGLFIVLSIVFFYIFAKVVSLKYGLLNTLAKTTT
                     RYSDNKNSNNRKSVFFALYKKEMGLFLNSYMAVLNAGLGVILLCVFSIFLLFNSLQQI
                     GEYSGIENINEYLSNFAPMCIASMFLLSCPAAFSISLEGKNVWILQSSPIRVKTILNS
                     KIAVNLTLHLIGYIVSVFIFLLKLDMDIIQTISLIIVPICYSIFITVIGISLNKKFPN
                     FEWESEMMIVKQSMPVIVSAIIGLAALVIPILFHWFLHFSIVPTLLGVALILLVIAGG
                     VYIKTCRLNF"
     gene            complement(9558..10286)
                     /locus_tag="VOX58_000944"
     CDS             complement(9558..10286)
                     /locus_tag="VOX58_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878179.1"
                     /translation="MLEIKNFSKFYGNKKAVENLSISVQSGDIYGFIGANGAGKTTTI
                     KAVVGIHDFENGSITIDGHSIKEEPVICKKIMAYIPDNPDLYEHLTGFQYINFIADLF
                     EVSTEIRRERIQRYGDLFEMTKHLSGMISSYSHGMKQRTAIISALVHSPKLLILDEPF
                     VGLDPKATFLLKKIMHECVSDGGAIFFSTHVLDVAEKLCNKIAIIKNGKLIASGNTEE
                     VKGNKSLETFFMEVQDNEKNLDTN"
     gene            complement(10398..11015)
                     /locus_tag="VOX58_000945"
     CDS             complement(10398..11015)
                     /locus_tag="VOX58_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HES5878180.1"
                     /translation="MRDVKEPEIRRAEIMDAAMILFMEKGYTNTTTQDIVDKVNISRG
                     LLYYHFKNKEDILYCLVEQYSDRLLKDIYIIAYDEDKTAIEKIRSFIDVTIISSENIS
                     AEGTVLQKTVDLKENQYMIDKLSHKLVEKLTIYFEKIINQGITEKVFSVKYPLETAEF
                     LMTAYVFVSNNISIRYLKKEPVNSYLNAFKVMLEQSLNTKKLFSN"
     gene            complement(11130..12899)
                     /locus_tag="VOX58_000946"
                     /pseudo
     CDS             complement(11130..12899)
                     /locus_tag="VOX58_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(12993..13496)
                     /locus_tag="VOX58_000947"
                     /pseudo
     CDS             complement(12993..13496)
                     /locus_tag="VOX58_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
     gene            complement(13560..15188)
                     /locus_tag="VOX58_000948"
     CDS             complement(13560..15188)
                     /locus_tag="VOX58_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878181.1"
                     /translation="MSWSTIWELIKINILYSNPQSLANLKKRQENHPKENFKAYKSMM
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVIQAPFSTAALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKAKQTKKPVNRPHQNQSLTQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDLKWQDMTQLFTRGNGQWLTMGLIFGNLIVTGVVGFGAVIIANIIQQ
                     PLLISILLSCLTLMVLGIAHLWIQKTFWKSLEKL"
     gene            complement(15193..15927)
                     /locus_tag="VOX58_000949"
     CDS             complement(15193..15927)
                     /locus_tag="VOX58_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878182.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            complement(15961..16227)
                     /locus_tag="VOX58_000950"
     CDS             complement(15961..16227)
                     /locus_tag="VOX58_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HES5878183.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            complement(16420..>17452)
                     /locus_tag="VOX58_000951"
     CDS             complement(16420..>17452)
                     /locus_tag="VOX58_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HES5878184.1"
                     /translation="YEAEYPELAAEYQKAFTNEAAQVELEAHELGSSMASRVSSQQAI
                     QQISEQVASFWGGSADLSASNNTMVKAETDFQPGHYEGRNIWFGVREFAMAAAMNGIA
                     LHGGTRVYGGTFFVFSNYLLPAVRMAALQNLPTVYVMTHDSIAVGEDGPTHEPIEQLA
                     SVRSMPNFNVIRPADGNETNAAWKRAIAETDRPTMLVLTRQNLPVLEGTKELAEDGLN
                     KGAYILSEAKGDLEGILIATGSEVKLAMDTQEALEAEGIHVRVVSMPSQNIFDEQSAE
                     YKESILPAAVTKRLAIEAGSSFGWAKYVGLAGKTLTIDTWGASAPGNRIFEEYGFTVA
                     NATELYKSL"
BASE COUNT         4919 a         3496 c         2586 g         6451 t
ORIGIN      
        1 ttagtagcca tatctaagtc agcttgagca tccacataga tatgcacatt ccccacacct
       61 gtttcgatga caggcacttt tgctttttcc ttaacggtct gaattaattt agcaccacca
      121 cgtggaatca acacatccac atagtccgtt gcctgcatca attcttcagc aacagcatgt
      181 gagggatcct caactagctg gacagcatct ggggtgatcc ctgatttttc cagactttgt
      241 cggatcaact tcaccaaagc tttatttgag tgaagggcat cttttcctcc ccttaggatg
      301 atggcattat tggttttgaa cgccagacta aaagcatcca ctgaaacatt cgggcgactc
      361 tcaaaaatca tcgcaatcac ccctagagga actctctttt ggaggatttt aaggccatcg
      421 aggttagtat agcccttaat gacttgtccg attggatcag ctaagtcagc aacctgttga
      481 actccctgtg ctatcgcttt aatccgttct gaagttaagc gcaagcgatc catcatgatg
      541 tcagaaatac catgttcttt agcattcgct aaatcttttt ggttagcggc taagagagtc
      601 tgtgtatcgt caactaatgc ctttgctaag gtgcttaaaa agcgattttt aatttgcgtc
      661 gacaaaggtg ctattaaaag actagcttgt tttgcccttt gccctaacct tctcatatca
      721 gtcatgtgtt acctcttcta tctgcttgaa ccaggtgcct agtggttgcc cttcaagcac
      781 tcttaaaata tctctgggat tagctccatt catcaagacc atctgtcctt tgttttcaaa
      841 taccatctga gcagactgaa ttttgctaag catcccacct gtgccaaatt tactgcccgc
      901 accacctgct gacgcaatga tttcttgcgt aatgactgca acatgactgc gtaactgcgc
      961 atcctcataa atagtagggt ttttatcaaa aaggccatca atatcagaca acatgattaa
     1021 taaatcagct tttgtaatac ctgtaacaac ttgtcaagga ctttctaatc atttttatcg
     1081 cctttttgat gtttttccat ctccatttct ctaagcatga tgcgttttaa tttgtcttgc
     1141 atggtttccg ttgcattttc attaaagtgg acaacaacct cataggttac tttcccaatt
     1201 ttctttatcg tctttgttcc tgtattctgt tcatttttat tttcttgcat atagttttcc
     1261 tcctgtttgt tgcaattaaa aaagacgata gatttttact tctaccgcct tgcttaaagc
     1321 tcatataact gtctattctg ttatccgcat attaaatttt caaattctta ttgtttaaca
     1381 ttccaccaaa cattgtatga tacatatcct tagaggattc ttctatcttg gtaatacttg
     1441 atattttatt tcctgcgtcc tttgatgaag ctccgcaaag ataaaaggct tcattttctg
     1501 ttccatagtc tattgcaata tatctatcgt gatatttttt gcctgcaacc ttcattttta
     1561 agttgatatt tggataatcc cttctaaaat catttaggat gttttttgta agcatatctt
     1621 tatttttcac attgtcgcta aagactataa tttcaacatt gtcttttgca gctcttaata
     1681 attctaaggt tttcaagcct atatagttat ctattacata aatgcttttc tttgctgact
     1741 tatatatttt tgtataggca acatccgctt caatcttatc tccattcatc aaaaggaaat
     1801 gcttgtaagt gtccggatcg ataaaattat ccattacttt tttcaaatct tctttagtag
     1861 ccatttgaga ttttatttct gcaatatcat ttgtgttttg gttggtttga atagctattt
     1921 ggactaattc ttttgaaccg ataaaatctt gattttcaat aatgaagtct ttcatctgct
     1981 taaatgttct aattaaggct ctgctttgcc taatcgcaag ttctcccctt aatacagtca
     2041 taagcatata aataccttgt tccgtaaagg catttggtag gtaccttgag ccgccccaac
     2101 ttgaggtgaa atttttgcac ctcaagtttt caaactcatt ctcggtcaat tgaaacataa
     2161 aatcttcgcc ttcaaacttt tcaatattat tttttacctg tctattaaag tttttagttt
     2221 cataaccata tatttcagct aaatctgcat caagcattac tttttgtcca cgaatgaaat
     2281 atattttttc ttgtatagtt ttatcatcta caataactat ctctttattt tcatctgcca
     2341 tgaaatgcac ctccaaaatt atctaaatat cttttccaat ccgccacgca ctgcgtagct
     2401 ttttgaaata agtctcatat ctttttatat ttttctactc caccttcagc ttttccgatg
     2461 tgttctacat ttttcacaag tcccttattt acattttcat aaaaccatct gccaatcaaa
     2521 gccggcggtg ttgctattaa ctctcgtaaa acaaattctt tgtctttcgg caaaaagctt
     2581 attttattta gtaaggtatt atataagtca acataacttg tattttcttg taaactgcta
     2641 cacttacaat attcactaat acttgggtaa ttaccctcta taactttttt agttaaaatt
     2701 ttcagttctt catcgtttac tgtaaattga attcgcattt atatatcctc tctttataac
     2761 tgagtttatt atcatatcac tgtcatttta tcataaatac gatgattttt ccatactgca
     2821 attttaagcc ctcacatgat gttttcacac ttagctatat tctgatacct cttttaccat
     2881 taacgctcta aaaaagcctt tccaccgtct tatatgagat tataaatgtt ctcccttgtc
     2941 ataatcttca agggtatata ctgttgattt actgcactcc tttaatttcc ctttatcttg
     3001 ttcataccaa tgaattaagg tcgcataatg gtctttgtag tttcttcctg tgctttgcat
     3061 ataagtcgaa agcttttcta tcattttctc cctatgacct tgcattttat cctttaactt
     3121 tccatattct tcatctgtta aggaaacatt tttatattct ccgtaaaaaa cgggggatat
     3181 tttttctatc tccccctctt tttctaactc tatatctatt tctttctcta tatctccgtt
     3241 gcaattttgt tgcaaaaggt tgcaatgtgt tgcattagtg ttgcattgca acgctttttg
     3301 tcctctcgat tttctcgaac gccttgtaga cgctgtttct gaacctataa gacttgggat
     3361 ttctgttaaa aaatactcgt cattttctgt gattatctcc atcaatcctt gtgccataag
     3421 atagttcact gtaaccatta catcatcttc tgtttcatca atcgttaagg caagctcttt
     3481 gataaagtct gtttctactc cgtcataata cagttttcca ctatctttta ggctaagcaa
     3541 aagcaatttg aggtaaatga ttgtataggt atctcctcct gatattcttc ttagtctttt
     3601 tattctcttg tctgtaaaaa aatcttcttt cagctttaac caatagtatc ttctgttatc
     3661 tgccattttt ctctcctctc tttttctttc aaaaggaagt cgtgatttac gacacccttt
     3721 atagttagcc cgccccaaaa cagggcatcc ttataaacct accatcgtga aatacgacac
     3781 cagttatatg catcgctaaa acgaggactc atctttatct atgcgttccc caaatgggta
     3841 cgcagcctga tacaccgaaa tagcgtgtca gcttattcat ccgaccgaac gatttgttcc
     3901 gtcagctttt attttttatg ttgtcctatt ttaggatttc atctttcttg ttctgtatat
     3961 ttttcttttg ctttttgttt gtactgctcc tttctttcct gttgctcttg gaattttctc
     4021 agttgtgcaa ttacacttgg cttttctcct cgcttaatcg ccttgtctat ctcaatactt
     4081 aggtctatgc cataatcgtc attgactgtc ttgactgcat acttgatatg gttgatactt
     4141 tcgtactctt ctttgattga ttgtgattgt ctgttgagtt tcgatatttc atcttcaagg
     4201 ctttggattt cttttttcca gtctttagac ttaatcgccg atgagcctgt tatcttttta
     4261 agaattgccc ttgctctttt atatttatct atctcatctt tatgggaatt gtaaaagctg
     4321 tctttgaata gggatttact gttccattcc tcaaagactt ttttgttatc cttgatgatg
     4381 tccgcatagg caaagcattt atttaagttt tctattcttg tggttttcgc cttaatgtct
     4441 tgactaatct tcttattttg ggattgtaat gtgcttatct tttcttgtag gtcggctatg
     4501 gttttaagat tgttgtcttt cagatatatt cttgctttgg aaaatcgtct taggtctgca
     4561 cctatctttt tgttgcttgc ataggggttt aactttcttg ctttttctcc ctgtaagtca
     4621 taataaatac tgatgtactc atagagatta aagagttccg ccttgttctc atattcttct
     4681 tttttctcct gtttgtattc atcatatttt acttgcagac ttccaagtaa cgagcctatc
     4741 caagatgtaa gtttggatag ctcctctttt aattttctaa attctaaatt caaggcgatg
     4801 atttttctgt tttggtttcc tctctctgtc tgtatgcctt ttttctccat ctgataactg
     4861 cttgtgccta aatgaatttg cgggagttct tctctgcctt gttctttaaa ggtgcgtgga
     4921 tctatccttt tatttatgtt gttccttgcc aagtattcgt ttgctttctt tgaaaagttt
     4981 tctctccact cttttgcttt gtcaggttca ttccaatcgt tcaaatttac ttttcttgat
     5041 ttatagtttc cgcttttcag ttttatcttt tctccgtttt catcaaggat atattctttt
     5101 ctgcatttcg gtttccaatt ccctttttcg tctatttctc taagggtaag taggatatgg
     5161 gcgtgtggtt gttcttcatg atcgcttgcc ataggattat gaatattaca gtctgctatc
     5221 atacctttag aagtaaagtt ttcttctata aattcgctga ttaactttat tctgtcctgt
     5281 tcatttaatt ctcttggaag tgtaaagagc agatttcttg caagttgtga atttttactt
     5341 ttctctattt tttcaacact attccatagg tattctctgt ttgaaaactc tttcgggata
     5401 tgttccggca gaaatatttt tgagaataca aggtcttctt ttttagaata gtcatgtgtt
     5461 tttccatagt attcatcttg taatttatct cttgcattgt atgcagcttt tgcaatcacg
     5521 ctatgccctt ttgaccttgc cacaatatct acatgggtat gcaaactttt gatttccatt
     5581 gtcttttcct cctttatctg ttttcaattt tgggatttta ctatcctaat gttcaggtcg
     5641 tatcttcgct ccctttttca aagggcgcaa agatacaccg tttgactacg gtgctttgcg
     5701 ttcctgcgga aagcttatag ctccgctaag gaaacaaaca cgctaaagca tttttatttg
     5761 ttcaaggggg ctactccccc ttagacccct gtgaactttg ttttttgtag atttttcaaa
     5821 tccacaaaaa agcaaagtgt taaaatgttt ctcactttgc tccatctcag tttctaatac
     5881 attttctgtt tttcttattc ttcctctgtc agttcttcaa aatcatcatt tgcattttcc
     5941 tctttaagat ttggattttc ttctaaggag atttcttttt cttttaaatg aatttctgta
     6001 agttctttta gcttttctct atttcgatta tcggaaagta cataatctaa aaaggcatag
     6061 attaaatcat tgtcggtact ttcttctgct ccggttatct gtttttcaaa tttctcaaac
     6121 acgcctcctt tttctacttt tcgtttcgtg tccgcctttc tttttagctg tgatttcttt
     6181 tgtcttagtt gcttaatttt gttttctgcc tgttcttttt tcttagtttc tttttccaac
     6241 tggctcatta cattttctaa ctctttcatt tttcattcct ccatttcaat attgttgtgt
     6301 tccaaacaaa gaaaggctaa gcacctttat ttgatactta acctttcagc attttttaga
     6361 atttatcttc taaaagtttt cttagttttt ctaaaatttt atccctcctt tttccgattg
     6421 cttgatagct tactttttct tttttgccaa tcgaccttag actttcttct ctgtaaaaga
     6481 tggcttccat taactctctt tctttttttg tgaatgtagc taacgccttg tttaattctt
     6541 caatttgcat tttgacctct acaatttttt ctaagtctat tctttcatct atgatgttat
     6601 ctacaaagtg tccgtcataa tccattgctg aaaatggaat cacgccatac ttccaatcct
     6661 ttctctgaag atatttttca tgctctgtta tcttccaata ggctttgtag acttcttcac
     6721 ttacgggtac agccttccct ttgacataaa ggaaatattc ttttttgtcc attgtgtaat
     6781 cctccttttt ctgctctctg tttttgcttt ttgagaataa aaaaggagga cttctacact
     6841 ttggcgtaaa atgtcctctt aggttaaaaa ctaatacttt atatgatttt ctactttgtt
     6901 ggttttgata aaatgatatt gctatgtgta agcaattaca aacataaaaa aggataaata
     6961 tatcttatgc taataacaga atttgatgtt cccattattt atccctccat aaaacttata
     7021 taaatcaaat cttcctccct tcttagatta gtttttccat gttaatcatt tatttttagt
     7081 tgcaacatat tcttcaaaca tcttgattat ctcagtatcc gaaaatccta actcatatag
     7141 tttttccaac aacatttttg ttagcctgtt caaatgctga tttcttactc aattattttc
     7201 ttctcacttt cagtaacaaa atatccttta cttccacgac taacaatcaa cccctgtttc
     7261 tcaagaattt ttaatgcttt ttggacggtt gcaggattac atccctccct ctgtgctaaa
     7321 gcccttatag gtggcatctg ttgttccgta atataatcgc ctcttaaaat agacactctt
     7381 aacgctgaag caatttgcaa atatagatat acattcattt atttttattt tttcttcctc
     7441 gtacgaattt ccaatatgca gaatatggaa taaccgcagc aaatagaaga taatatttat
     7501 tagcaaacat atcggggatt ccttttgcat tgaaatgaac aggaattttc tctgggagaa
     7561 atatgcaaac aataaatatt acaaacatta tccctatcaa caccaataca tctttcaaaa
     7621 atcttttttt catggcaaat ctcctttcta tttgtcaata gctttctatt ttcctaaaat
     7681 ttatctaacc accttataac ttctacaaat gtatccctat taattgaata atagatataa
     7741 tttttatatc tactgtcggt aattaattta ctctttttta ataaagatag atgataagat
     7801 atagttgctt tttctaattt aaaatgactt gctatttctc cagcagttaa atttttatta
     7861 gaaataatta ataaaatatc cattctaatg ggatttgata gggaatcaaa tattctaact
     7921 aaatctatca tcttttattc ctgaaatatt gcaaataaac ttaatattga aataaacacc
     7981 attatatctt ttaataattt ttttcataga acatctcctc cttaaactaa aaatttaatc
     8041 tacaagtttt tatataaacg ccacccgcaa taacgagcaa aataagtgct actcccaata
     8101 atgtaggtac tatagaaaaa tgcaaaaacc aatggaaaag aattggtata actaatgcag
     8161 ccagtccaat aattgcagat acaataacag gcatactctg ttttactatc atcatctcac
     8221 tttcccattc aaagttaggg aattttttat tcaaagaaat tccaatcact gttataaaaa
     8281 tggaataaca aataggaaca attattaagc tgatggtttg aataatgtcc atatcaagct
     8341 ttagcaaaaa tataaacaca gaaacaatat atccaattaa atgaagcgta agatttactg
     8401 caatcttaga atttaaaatc gtttttaccc taataggaga actttgcaaa atccaaacat
     8461 ttttaccttc taaagatatg gaaaacgctg ccggacaact aagtaaaaac attgaagcaa
     8521 tacacattgg agcaaaattt gaaagatatt cattaatatt ttcgattccc gaatattccc
     8581 ctatttgttg taaagaatta aaaagaagaa atatactaaa aacacataaa agtattacac
     8641 caagacctgc attcaacact gccatatatg agtttagaaa cagtcccatt tcttttttat
     8701 atagggcaaa aaatactgat tttctattat tcgaattttt gttgtcagaa tatcttgttg
     8761 ttgttttagc aagagtatta agcaacccat acttcaatga aaccactttg gcaaatatat
     8821 aaaaaaatac tattgaaaga acaataaata atcctactcc aatgtacatt ggaaaattat
     8881 agtattccat aaatagctta gataacgggt acaatccggt aatttgctta gaaagcataa
     8941 tcccaatact attttcatta cctgatttca tagctgacac cgcaatatat ccaattatcc
     9001 ctatcattgc aaatgaaaaa attaaagcaa taacattttt tctcttaaag aaagatgaag
     9061 caacaacaat aatgattccc atacatgctg ctatacacat aggtatcaac ggaacaaaaa
     9121 ttatggaagt aaaatacaat ataatctgta atacatttaa acttccgttt aacacccaaa
     9181 caattccccc cggaagcata aacatcaatc ctattaaaaa attcaataaa tacataaaaa
     9241 taaacttgct gcttataata tccgaacttt ttacaggcaa tgacaaaaga ttttccatat
     9301 cacgactgcc aaataaaatc ccattcgaat aaaatattgt caaaaataat attgcaaagc
     9361 ttgataccga taccatatat gccggtatca actcctgctg tcccagttgt accaatgttt
     9421 tggctgttat gatattataa acaaagaaaa atatagcaag agttattatt ccaaaacttg
     9481 caataacaat tgaactttgt tttttatttc ccggctcttt catttcgttg atcggaaaga
     9541 aatttatcaa ttgtgctcta attagtatcc aaatttttct cattatcctg cacctccatg
     9601 aaaaaagttt ccaaagactt atttcctttg acttcttctg tatttccgct cgcaattaac
     9661 tttccatttt ttataattgc aattttatta cataattttt ctgcaacatc caatacatgt
     9721 gttgaaaaga aaatagctcc tccatctgat acgcattcgt gcattatttt ctttagtaga
     9781 aaagttgctt tcggatcaag tccaacaaaa ggttcatcta aaattagtaa ctttggagaa
     9841 tgcaccagtg cagaaataat cgccgttctt tgcttcatgc catgtgaata cgaagatatc
     9901 attcccgaaa gatgtttcgt catttcaaat aaatcgccgt atctctgaat tctttctctg
     9961 cgtatttcag tagaaacttc aaataaatct gcaataaaat tgatgtactg aaatcctgtc
    10021 agatgttcat ataaatccgg attatccgga atgtatgcca ttattttctt acaaataaca
    10081 ggctcttctt taatagaatg cccgtcaatc gttatacttc cattctcaaa atcatgaatt
    10141 ccaactaccg cttttatcgt tgtagttttt ccggctccat ttgcaccaat gaatccataa
    10201 atatctccag actgtactga aatggataag ttttcaacag cctttttgtt accatagaat
    10261 ttactaaaat tctttatctc taacataatt tccacctcca ttgacaattt gtcagttatt
    10321 ccttgaaata aaaccgataa cttgtttagc agtagctatc ggcatattat tctccttgta
    10381 aacattctat tcttttttta attactaaag agctttttcg tatttaagct ttgttcaagc
    10441 attaccttaa atgcatttaa gtaactatta acaggttctt tctttagata tcttatactt
    10501 atgttatttg aaacaaaaac ataagctgtc ataagaaatt ctgctgtttc taatggatat
    10561 tttacagaaa aaactttttc tgttattcct tgatttataa tcttttcaaa atatattgtt
    10621 agtttttcga caagtttatg agataactta tctatcatat attgattttc tttaaggtca
    10681 acagtttttt gtagcactgt accctctgct gaaatatttt ccgaagatat aattgtgaca
    10741 tctataaaag atctgatttt ttctatggca gttttatctt catcataagc aataatataa
    10801 atatctttca gtagtctatc tgaatattgt tctacaagac aatataagat atcctcttta
    10861 ttcttaaagt gataatataa caaccctctt gatatattta ctttatcaac tatatcctga
    10921 gttgttgtat tagtatatcc tttctccata aagagtatca ttgcagcatc cataatctct
    10981 gcacgtcgta tttcaggttc ttttacatct ctcattttat acctcctatt atttatataa
    11041 tacatctaaa ctgacaaatt gtcaattgat tttaatcttg ttattttatg ctttttcata
    11101 gtcttttcaa aacctttagc ttctcctgtt tttttcacct ctctccttgc cttatatctt
    11161 tcctcatatt ctttttgctt gccgtttgct ttacgcttga ggtagttttg atgaagtcgg
    11221 tcttttcttg ctcgttcttt ttcttcctcc tctgttaatt tctgcatttc ttcttcggat
    11281 aattcttctt tcggcggttc ataattcccg ataaagttga agtagatttc gactttctga
    11341 ttggcggttt gactgccctt tcgatctcgt tcgtggacta agattttttc tacaaactcg
    11401 ttaatgattg tcggtgtcag ttcgtcaaag ttatcatagc gttcaatcaa ccggataaac
    11461 ttcttggctc tatcggtttc tttttcgtat cgcttgatgg ctttttccaa cccatcgatt
    11521 tctttgctaa gttccctttg ctctgtttca tactgattgt ttaagatttc atatcgggtg
    11581 ttcggtatct tctccaatat catatcttcg tatattcgac acattaagcg ttccagttcc
    11641 tgaagtctgt ttttactatc tgtgagcctt gtctttttct tttccatttc cattttctcg
    11701 gtttcttcca tttcgttttg aatggaatgg agaaaggctt cattatcttc atcaagataa
    11761 ttttttatat cttttaatgt ttcttggata aggtttaaga ctacttctgc ttttatcctg
    11821 tgagctgatc gacatagcgt tccgcaaggc actttcttat aattactgca aacataataa
    11881 ggaatattct tatagttatt tgtcctatga acatacatct tgctcccgca atctgcacaa
    11941 tacatcaatc ctgttagtgg gtgatattcg ccccaaccat caggatacct ttttacattt
    12001 cctcttatcc tttgcacatt atcaaaggtt tcttggtcta tgattgcttc gtgagtattt
    12061 tcaaagatga gccatttatc ttcggataca tatttacttt tcttatcctt gaaatgcttt
    12121 cttgttttaa agttaactgt atgccctaag tattcttttt tctttaagat actggcaatt
    12181 gtggaactac accaacgata aggatattca aagtttttgc tttgatgtaa tccatatccc
    12241 attttttgct gatgataagc gggtatatct atcttatctg cttccagtat ctttgctatt
    12301 ttgtatggtc ctgctccgtc cattgtaaga ttaaatattc gtcttactat ctcggctgct
    12361 ttttcatcta caacccactt atctttgtct ttttcatctt tgatgtagcc ataaggtgga
    12421 gtgcttgccg tatgctttcc gctttctcct tttgatctaa atgtagattg tatttttctt
    12481 gaagtatctc ttgcatacca ttcattcata atatttctaa aaggggtaaa atcatcttct
    12541 ctgtaaaagc tgtctacatt gtcattgata gcaatcagtc ttacgccctt ttgtcttaat
    12601 atctccatac attgaccgac tttgagataa tctctgccga gtctactcat atcctttacg
    12661 atgatacaac cgatatttcc agtatttact ccattcatca tatccataaa gcctggtcta
    12721 tcaaactgtg tcccgcttat tccgtcatct gtaaaatgga tgatgttagt taaattattc
    12781 ttacttgcat attcttcaag gattttcttt tgattaacga ttgagttact ttctccttca
    12841 agttcatcat ctcgacttag tctctcataa agtgctgtta tcttttcaaa attcctcact
    12901 ttttcctcct ttctcttaat ttcttaaata aaaaagaatt aagaagtaca tgtttcacta
    12961 aaacagtgat taagtgttct ccttaattct attactccag caacaacagc agataaacgg
    13021 tcattatctc caaatttagt cgcatgatcc atttcatcca cactaaccgc atcgttttca
    13081 ttaacaatcg gcacaatgcc caaactaatc agactttcaa aagcattggt aacattggct
    13141 agactttctg ggaaaaccac cacatctctc gtcaataaaa tttgtgacac gttggtttgg
    13201 tagtgtgcaa aaatttgtga ataaagactc atcatggcca cctgaccaac actggaaact
    13261 gcctgttgtt tagcaagatt ggtcggacgt ttttccattt tcaaaatatc tagtccaaat
    13321 cccatcgctc ccgatgatac taaaataact tccttgccct tattcatcaa acttgaaatc
    13381 acaaaagcca gttggtcgat tttttctaaa ttgattttac ctgttggtag cactaatgaa
    13441 ctagtgccaa ttttaattac gatacgtgtt acatcttcaa attgtcgttt catcataccc
    13501 aaattatagc ataaaaacag ccccagagga ctgtttttcc gaataatgat ctctcttcgt
    13561 taaagcttct ctaagctttt ccagaaggtt ttttgaatcc ataagtgagc aatccctaaa
    13621 accattagtg tcagacaact caatagaata ctaatcaaca gaggttgttg gataatattg
    13681 gcaatgataa cagctccaaa acctactaca cctgtgacta ttaaattacc aaagataagg
    13741 cccattgtta gccactgccc atttcctctg gtaaagagtt gtgtcatgtc ttgccatttt
    13801 aaatccagaa gccggtagtc acgacgatac atcaattccc cttgcacaat cagagacagg
    13861 gcgtagccaa gacaaaaagc aatcgttaga agaggatgca ggtgcaacac aaagagacca
    13921 aacacaagat aaatcaccat aggcacaatc agttgcagac cgacgagcaa gcaaaattta
    13981 tccagcaaga atttttttaa cgttattggt aagcttttaa taaaggtaaa gttatccttt
    14041 tcaagtgaaa tacctactcc aataaagctg gttggtgttg cacacatgac acccaaactg
    14101 accccaaata ataaggccac cccaaagtaa tctggggaaa tatgcttaaa gaaacctgtg
    14161 ccacgtgaca aacttggacc gataaaaagc atcacataca tcaggggcat gagataggtt
    14221 tgtgtcagta aagttgcatt ttgtaacgtt aaaagatgat gttttcgcaa caactgcgtc
    14281 agtgattgat tctgatgagg acgattaact ggttttttag tttgcttggc tttgttctcg
    14341 ttactaatat aaaaagcctc acgataataa gttggcatca cttttgtgac aataccatat
    14401 accatagcta aaatcagtag caatggcaac caaaagttaa ggagggccgc agtagaaaac
    14461 ggtgcctgaa taacatcata gaatcctttg aaatacggaa tagttgggta atcagtaaat
    14521 acgccatccg tcatcgtacg cttgttgtta ctaatattaa tcgcaaaaat taagacgaaa
    14581 gctccaaaag ttgagacaaa catcatgata gttgaaatga gtttccgctt gcgactcctt
    14641 acaatgattt tgcccaccca agcattgata taaatagcta ataccatcga actcactagt
    14701 aacactaaaa agagcacaat agctactaaa atagacaaag gatttcccaa tagttgccaa
    14761 taggcaatta agagaagaga gataagaggc atcaaaaaga cagcacccat ccctagcgac
    14821 gagacaattt ttgcaatata gagttcttct gacgttactg gtaagtgaat ataaagtttt
    14881 aagtcattac tttcataaaa aatggtgtaa agagagctaa aggctgtcaa ggttgacatg
    14941 ataaaaaaca tagcaacgtc aaaggagaaa agtcctggat aatgactgaa atcgacacca
    15001 ataaacataa aaaggtaaat caccaaaaac atggcaatca ttaaggcctg ttgcctcatc
    15061 atgcttttat aggctttgaa gttttctttc gggtggtttt cctgacgctt tttgaggtta
    15121 gctaaacttt gtggattgga ataaagaatg tttatcttga taagttccca aatagtagac
    15181 caactcatcc catcacccct cttcttgcgc cttacgacct gcgagttcaa ggtagatgct
    15241 ctccaagtct ttgtcaggat ggtgttcttt caactcatca atcgttccaa caaaaatcaa
    15301 tttccctttt tttagaatcc cgatacgatc acataactgc tcagcgactg atagcacgtg
    15361 agtcgaaaat aagaccgtat gtccagaagc tgcatgagct ttcatcattt ccttaaggtc
    15421 aaaagaagcc tgaggatcca aacctgttaa tggttcatcc aaaatccaga tgttaggatt
    15481 tgatacaaga gcaccaatca caatcacttt ttggcgcatc ccatgtgaga aactatcaat
    15541 ggtttgatta acttcttcct tcagttcaaa aagtgttgtc agctgtgcta accgttcttc
    15601 tcggtcctca tctgacaccc cataaatttt agctaaaaac tgccaatact catttggagt
    15661 tagattcaag aaaatatcag gagaatctgg cacataagca atttgttttt tgatagcttc
    15721 tctattttct gtcagaagtt gaccatccac aaatacttcg ccataactcg cttcgataat
    15781 agaagtcaaa atgctgatgg tcgtggtttt acctgcacca ttatgtccga taaggccaaa
    15841 aatttcaccg tcattgatgg tgacgtttaa atcgctgaga gcttctttgt cgccatacaa
    15901 ctttgaaaca tgtttaaatt ctatcatggt atcctttttc cttttcttct ctttttctcc
    15961 ctagactgat gaggcttcct ctaaaatata cttttccaat gactgcttaa aacggcaaag
    16021 cgattttgga agtaaaatgt cattatcaag taagtaatta tccacatctt ggtttagttg
    16081 gtaggcaaca agaagtgggt tactgtgttt aattaatcta ttagaagctg ctaaaagtag
    16141 ctgcgacaag gccacatttt cacggttttc tggataatca taagcctgtc taatcacatc
    16201 gtataagcgc tgtcgtttct tttccattta tactccttta ttttttgtac ttttacgcta
    16261 atacaataac aattaaatta taatttttat gaatcgtttt gtcaatgact tcttatcaaa
    16321 agaagtgtat taggcagttt gaggaaaaga aagaactggt tattttattt taaaaaaaca
    16381 gagtaaatca tttgatttac tctgtttggg atgtgtaacc tagagtgatt tgtataactc
    16441 agttgcattt gccacagtga aaccatattc ttcgaagata cgatttcctg gagctgaagc
    16501 accccaagtg tcaatggtca atgtttttcc tgctaggcca acatattttg cccagccaaa
    16561 gcttgaacct gcttcgatag cgagacgttt agtcacagct gctggtagga tgctttcttt
    16621 atactctgct gactgttcgt caaagatatt ttgtgatggc atagatacga cacgcacatg
    16681 aatgccttct gcttcaagag cttcttgagt atccattgcc agtttcactt ctgaacctgt
    16741 tgcaatcaag ataccttcaa ggtcgccttt ggcttctgac aagatgtaag ctcctttgtt
    16801 aagaccatct tctgccaatt cttttgtgcc ctcaagcact ggaaggtttt gacgcgtcaa
    16861 tacaagcatg gttgggcgat ctgtttcagc aatggcacgt ttccaagcag catttgtttc
    16921 gttaccgtct gctggacgaa taacgttaaa gttaggcatt gagcgaacac ttgccaattg
    16981 ttcaattggt tcgtgcgttg gcccatcttc accaacagcg atagaatcat gtgtcatgac
    17041 ataaactgtt ggcaagtttt ggagcgctgc catacgaaca gctggaagaa gatagttaga
    17101 gaagacaaaa aaggttccgc cgtaaacgcg tgtcccgccg tgaagggcaa taccgttcat
    17161 ggctgcagcc atggcaaatt cacgaacgcc aaaccagatg ttacggcctt cgtagtgacc
    17221 tggttggaaa tctgtttcag ccttaaccat tgtgttgtta gatgctgaaa gatctgctga
    17281 tccaccccag aaagaggcta cttgttctga gatttgttgg atggcttgtt gactggatac
    17341 acgactcgcc attgaactac caagttcgtg cgcttcaagt tctacttgag cagcttcgtt
    17401 agtaaaggct ttttggtatt ctgctgctaa ttctggatat tctgcttcat aa
//
LOCUS       DAUVUY010000032        17285 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.032, whole genome shotgun sequence.
ACCESSION   DAUVUY010000032 DAUVUY010000000
VERSION     DAUVUY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17285
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.032"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..647)
                     /locus_tag="VOX58_000952"
     CDS             complement(<1..647)
                     /locus_tag="VOX58_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HES5878185.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMVSLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLID"
     gene            complement(781..1734)
                     /gene="manA"
                     /locus_tag="VOX58_000953"
     CDS             complement(781..1734)
                     /gene="manA"
                     /locus_tag="VOX58_000953"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HES5878186.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATIVLDNMVETTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
     gene            complement(1829..2713)
                     /locus_tag="VOX58_000954"
     CDS             complement(1829..2713)
                     /locus_tag="VOX58_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HES5878187.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFLTTTPEETIART
                     IAYFKAFEADLAGMAIGSFGPIDIDPSSETYGHITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     PHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMTQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            complement(2977..5991)
                     /locus_tag="VOX58_000955"
     CDS             complement(2977..5991)
                     /locus_tag="VOX58_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HES5878188.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKAGQESQKVKEILAKAQQADKQAQELAKMKIPE
                     KIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLF
                     WKELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIV
                     DEYVYKYNLDGLDVDVEHDSIPKVNGEASDENLKRSIDVFEEIGKLIGPKGADKSRLF
                     IMDSTYMADKNPLIERGAPYIDLLLVQVYGARGEQGEFQNDTKLVTDTPEERWQGYSK
                     YIRPEQYMIGFSFYEERAGSGNLWYDINSRKDEDKANGINTDITGTRAERYARWQPKT
                     GGVKGGIFSYAVDRDGVAHQPKQIAEKDKQNVKNNQPQIPEITDNIFHSDYSVSKALK
                     TVMLKDKSYDLIDEKDFPDKALREAVMAQVGTRKRDLERFNGILRLDNPAIQSLEGLN
                     KFKKLAQLDLIGLSRITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSL
                     KVSGLTGLKELDLSGFDRETLAGLDVATLTSLEKVDISGNKLDLAPGTENRQIFDTML
                     STVSNHVGSNEQTVKFDKQKPTGHYPDTYGKTSLRLPVANGKVDLQSQLLFGTVTNQG
                     TLINSEADYKAYQNQQIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLAT
                     DKEETYKVDFFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADPVNARKVFD
                     GQLGSETDNISLGWDSKQSIIFKLKEDGLIKYWRFFNDSARNPKTTNKPIQEASLQIF
                     NIKDYNLDNLLENPNKFDDEKYWITVDTYSAQGERATAFSNTLNNITSKYWRVVFDTK
                     GDRYSSPVVPELQILGYPLPNADTIMKTVTIAKGLSQQKDKFSQKMLDELKIKEMALE
                     TSLNSKIFDVTAINANAGVLKDCIEKRQLLKK"
     gene            complement(6222..8105)
                     /locus_tag="VOX58_000956"
     CDS             complement(6222..8105)
                     /locus_tag="VOX58_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HES5878189.1"
                     /translation="MDNRQIATEVIEALGGRENVRSVAHCATRLRVMVHDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSTLGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGHLLQYLVTMLVGLGVAFGIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            8347..9786
                     /locus_tag="VOX58_000957"
     CDS             8347..9786
                     /locus_tag="VOX58_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HES5878190.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGHYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            9791..10756
                     /locus_tag="VOX58_000958"
     CDS             9791..10756
                     /locus_tag="VOX58_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HES5878191.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINQKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEIIKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(10897..11349)
                     /gene="nusB"
                     /locus_tag="VOX58_000959"
     CDS             complement(10897..11349)
                     /gene="nusB"
                     /locus_tag="VOX58_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HES5878192.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(11342..11731)
                     /locus_tag="VOX58_000960"
     CDS             complement(11342..11731)
                     /locus_tag="VOX58_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HES5878193.1"
                     /translation="MTTEHIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            complement(11777..12334)
                     /gene="efp"
                     /locus_tag="VOX58_000961"
     CDS             complement(11777..12334)
                     /gene="efp"
                     /locus_tag="VOX58_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HES5878194.1"
                     /translation="MIEASKLKAGMTFESEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            complement(12422..12889)
                     /locus_tag="VOX58_000962"
     CDS             complement(12422..12889)
                     /locus_tag="VOX58_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HES5878195.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGIKKITYKAHYRPHPFAIELMEKKGVAYVQHDVPHIILGGKEGPNG"
     gene            complement(12923..13996)
                     /locus_tag="VOX58_000963"
     CDS             complement(12923..13996)
                     /locus_tag="VOX58_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HES5878196.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLEADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRFIKDTSEIDTIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIVASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIDKASAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFFGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITETGCQVLTLAPKELIVL"
     gene            complement(14112..16940)
                     /gene="uvrA"
                     /locus_tag="VOX58_000964"
     CDS             complement(14112..16940)
                     /gene="uvrA"
                     /locus_tag="VOX58_000964"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HES5878197.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLREGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVIYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            17143..>17285
                     /locus_tag="VOX58_000965"
     CDS             17143..>17285
                     /locus_tag="VOX58_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HES5878198.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISD"
BASE COUNT         5109 a         3873 c         3114 g         5189 t
ORIGIN      
        1 tcgtcaatta agactgagtc cacttcatca accaaggcaa aattgagtgg gcgttgtacc
       61 atgtcttcct ggcgaacaac catgttgtca cgaaggtaat caaaaccgac ttcagagttg
      121 gttgaataag taatatcaca attataagct tcacgttttt cagcaggaga cttagcagct
      181 aggttgatcc caactgaaag acctaaccag ctatacactt cacccatttc ggtagcgtca
      241 cgcgttgaaa gatactcatt aacagtaata acgtgaacac cctcaccagc aatagcattg
      301 agataaactg gcatggtggc tgtaagagtt tttccttcac cagtacgcat ttcagggaca
      361 tccccgttat gaagaacaat tcctcccata atttgaacac gataagggaa caagcccaac
      421 acacgttttg ctgcttcacg aacaactgcg aaagcctcag gtaatagttg ttcgagggtc
      481 tcaccttttt gatagcgttc tttaaattca agtgtttttc cttgtagatc tctatccgac
      541 aatgacacca tttggtctgc gtaggattct actttttttg caattttttc taattttctt
      601 agttcgcctt tgtcattttc gataactttg cgtagaatat tggccattct ctttcctttc
      661 gatcatctaa tcatttcatt ttaacataaa tatgtaggca gttcaagaaa ggttggtaaa
      721 gttatcagag aaacagagag tcataatttt atcctttttt ctcacattga taccatggta
      781 ttaaacgagt tgattggggt gactcacaat catttctaat tgaccatcaa atgaccacga
      841 tttaacatcg ttaggcaaga taaagtgcat gcctttttct agctcataag ccttctggtc
      901 cacatagagt tttccttgac cttttaaaac actcactaaa agataaggcg cagcttgttt
      961 catgtcaacc atttgactag ttacccattt gtaaacagtg aaaaatggcg ttgagactaa
     1021 agttgtttct accatattat ccaaaactat agtggctggc acgctatttt ctggcttacc
     1081 aatcgttaag acatcaatag acttttcaat atgaaggtca cgaagattac cattaacgtc
     1141 cttacggtca aaatcataaa cccgataagt ggtatcagat gactgctggg tttctaaaat
     1201 aagaattccc ttgccgatcg catgcatggt gccacttgga acatagaaaa aatcgcctgc
     1261 tttgactggg acacgagtca gcaaatcatc ccaagcgcct gcttcaatca tggcacgaag
     1321 gtcttctttg gacttggcct gatggccgta tacaatttca gaaccttctt cggcagaaat
     1381 aatataccag cattctgtct tgcctaattc tccttcatgt tcacgtccat aagcatcatc
     1441 aggatgaact tggacactta gccagtcatt agcatctaaa atcttagtta acagtggaaa
     1501 aacttcttcc tttggattcc caaacaaggc cggctcttgc gcatataatg tatttaaagg
     1561 ttgtccttga taacgtccat tagtaactgt cgaaacacca ttaggatgag cagaaatagc
     1621 ccaatactca cctgtggtgt cacttgggat gttgtaagca aagacatctc tcaacttggt
     1681 gccaccccaa attctgtcgt gcatggttga ttttagaaat aatggttctg acataatatt
     1741 cctcgcttct gatgttaatc tctgtttatt atagcatgtt ctagctgact gcgctttctt
     1801 ttactagaca agcaggcaaa aacagaccct attttccttg tgcagccaac ttggccaaag
     1861 caaaatttcc cagagtagcg gagccattat cagcaacagc tggagtaaca atgtagtctg
     1921 tcaaatcagg gactggcaaa taaccgctta aaagggtagt gaatttatca tgcacgcgca
     1981 acaccatatg ttcttgcgtc atgacacctc cgccaaaaac aatcacttga ggacggtaaa
     2041 gcatagttgc ttgcagcgct gcttgggcaa tgtagaaggc ttgaatgtcc caaacatcag
     2101 cttcctgatc aagccgttca cctctcactc ccgttctggc ctcaatactt ggacctgctg
     2161 ccatcccttc cagacaccct ttatgaaaag ggcaaacacc taaaaatccc ttggccatat
     2221 catcaggatg gggcatgaca taggtatgac ccgcttctgt atgacctaat cccccaataa
     2281 agtggccatg ttgaatagct cccgccccaa taccagtacc tatggtgtaa tagaccaaac
     2341 tttccacacc aggacgagca agcacctcac cataagcaga gctattaaca tctgttgtca
     2401 catcaaaagg aatcttaaaa gcagccgaaa gttgtcctaa caaatccaca ttagcccagc
     2461 ctgacttagg agtggttgtg atatgaccgt aagtctctga cgacggatca atatcaattg
     2521 gtccaaaaga accaatcgcc attccagcca aatctgcctc aaaagcttta aaataagcga
     2581 tggttcgcgc gatggtttct tctggtgtgg tggtcagaaa ttgtgtttta tccaccactg
     2641 taaactcctc atcacccacg gcacagacaa actttgtccc accagcttca atgcttccat
     2701 ataattttcc catgttttct cctttttctt gttctttttt atctttatga tgacacaggc
     2761 tcttatcaag acagccatgt ctcacggaca agtgccactt tctggtagtc taacgattat
     2821 ttagctttat gtcatcaaaa cctataaaag actaaaaatc tgggactatt catctcagat
     2881 ttttagtctt ttggttaatc aattccagca aacctttatc atagtcacat tttgttaatt
     2941 taatctgaat gttgctatct aagaaagttg ctttgtttat ttttttagca gctgcctttt
     3001 ttcaatacaa tctttcaaaa ctccagcatt agcattaata gcagttacat caaaaatctt
     3061 actgttcaaa gaagtttcta aagccatctc ttttattttt aactcatcaa gcatcttttg
     3121 agaaaactta tctttttgtt gagatagacc tttagcaata gttactgttt tcatgatagt
     3181 gtcggcgtta ggtaacggat aacctaaaat ttggagttca gggactactg gcgaactata
     3241 tctatctcct ttagtatcaa agacaactcg ccaatattta ctagtaatat tatttaatgt
     3301 attactgaat gcagtagctc tctctccttg tgcactgtaa gtatctacag taatccaata
     3361 tttttcatca tcaaatttat tgggattttc caacaaatta tctagattat aatctttgat
     3421 attaaaaatt tgcagacttg cttcctgaat aggtttattg gttgtcttag gatttcgggc
     3481 tgaatcattg aagaaacgcc aatactttat taatccatct tctttcaatt taaatataat
     3541 actttgctta gaatcccatc ctaaagagat attatcagtc tcactgccca gttgcccatc
     3601 aaataccttt cttgcattta caggatcagc gcttcctcca ataactgttg cgccttctgc
     3661 caaattaacc atcatggttt tttcgtcacc aacaatcact ttagcagtat gaacagcttt
     3721 tgtcttatct gctgggctaa agaagtcaac cttataggtc tcttctttat cagtcgctag
     3781 cgttttgtca gtagtggttc ccaatgtgga atcagttact ttaacggtat agttctcata
     3841 agaaacttta aagttattgt aatgatagtt tgaatcaaca aagctacgtc cagcaatctg
     3901 ctgattttgg taagccttat agtctgcttc gctattgatt agggttcctt gattcgtcac
     3961 agtcccaaac aaaagctggc tttgcaaatc aacttttcca tttgccactg gtaagcgcag
     4021 actagttttg ccataggtat ctgggtaatg cccagttggt ttttgcttgt caaatttcac
     4081 tgtttgttca ttgcttccaa catgattgct gacagttgat agcatagtat caaaaatttg
     4141 tcgattttct gttcctggag ccaaatcaag tttgttgcca gaaatatcaa ctttttctaa
     4201 agacgttaga gtagcgacat caagaccagc caaggtttca cggtcaaaac ctgacaaatc
     4261 taattctttc agaccagtta aaccagaaac cttcaaagat actggtggga tagtagcagg
     4321 ttcttctttg ttatcctttt tataggtttc aagaactgtt tccaaggtat ctttgcctgg
     4381 cttcatatta gcgggtaaaa cagattggtc gagctttgta atgcgagata agccaatcaa
     4441 gtctaattga gctaattttt taaatttatt tagaccttct aaactttgaa tcgctggatt
     4501 atccaatcgt aatatgccat tgaaacgttc caaatcacgt tttctggttc caacctgcgc
     4561 catcacagct tctcgcaaag ccttatctgg gaaatctttc tcatcaatca gatcatacga
     4621 cttatctttt agcataactg tctttagtgc cttggagaca ctataatctg agtggaaaat
     4681 gttatcagta atctctggaa tctgaggttg attatttttt acattctgtt tatctttttc
     4741 agctatttgt ttgggttgat gagctacacc atcacgatcc acagcatagg agaagattcc
     4801 tcccttaacc ccacctgttt taggttgcca ccttgcatac cgttcggcac gcgttccagt
     4861 tatgtcagtg ttaattccat ttgctttgtc ctcgtccttg cgagaattaa tatcatacca
     4921 aagattgccg ctgcctgctc tttcctcata gaaagaaaaa ccaatcatgt attgttcagg
     4981 acgaatatac ttgctataac cttgccatcg ttcttcaggg gtatctgtaa ctaacttggt
     5041 atcgttttga aactcacctt gttctcctct tgcgccatag acttgtacca ataacaaatc
     5101 aatataagga gctcctcgct caatcaatgg gtttttatca gccatgtagg tgctatccat
     5161 aataaacaga cgcgatttat cagcaccttt tggtccaatt aatttcccaa tctcttcaaa
     5221 aacatcaata gatcgcttga gattttcgtc actagcttct ccatttactt tcgggatact
     5281 atcatgttca acatccacat ctaagccatc aaggttgtat ttataaacat attcatcaac
     5341 aatagctttg gctaaagctt tatttccctc tggtgtattt gggtatttac tggtatcttc
     5401 tgcaatacca ctgttatcac ctccagctag gaaacgccat ggaatggtac gaatgacacg
     5461 tgtcccttgc ttgtttaact ttggcacatg tttggtggcc aattctttcc aaaaaaggct
     5521 ataatctttt gtccaatcgt ggaaaataaa ggctagatct acttctttag gaagctctcc
     5581 catcgagtta actttgtctt tttctgttgg atctgatgtt ttgtcatgcc aagttctaaa
     5641 gtaaccaccg tagagaggac catgtaacgg tttcatcggt attttctcag gaattttcat
     5701 tttggcaagt tcttgagctt gtttatctgc ctgctgagct tttgctaata tctctttgac
     5761 cttttgagat tcttgccccg cttttgagag ttcttcttta aattcttttt tgctattctc
     5821 tgacagataa tgcaagctat ctatagaaga taattctttc tgaacctgaa cagtcttctc
     5881 ctccgctttt acagtattga gtgaatcatg gtgggtcgct aaggcagctc ccatcaacgt
     5941 tgcagcacaa acacacccta gtgttctttt taccaacaaa tgtttatcca tttggacact
     6001 ccttattttt ggtactaagt catggcttcc cagttgatat accatctatg taagcgcatt
     6061 cctatagttc gccttaagta taacaacact ttattaaaaa aaacaaggta caaagtatag
     6121 gtaaaataac acaaatttaa ggtttttagg cttttctcat ttttttgcca acctgccatg
     6181 aatggcagga caaaagagct gcttagttag ctcttttgtc cttatttgat aagtgccact
     6241 tgatcaccaa tcaaaacatg tccttgagca agaatgtcca cgctttgata gtcagcgcta
     6301 ttggtcacaa tcatcatggt ggtgtcatct agtccagctt ctgcaatctt gctaggatca
     6361 aagtgtccaa gaagatcacc ttttttaacc gcctgaccta ctgctacaag agattcaaag
     6421 ccatctcctg ccatcgacac agtgtcaata ccaatatgaa gtaacacttc tgctccttga
     6481 cttgacgtta tagcataggc atgacctgtt tcaaagacaa tttcaacttt accatcaact
     6541 ggtgagtata gggtattatc ttctggctta attgctaagc cttgacccat agcacctgat
     6601 gaaaaaacag ggtctgaaac agctgctaaa tctacaactg ttccattcaa aggactatat
     6661 aaagtttcct ctgctaaggc tggctgatca gccgtttgat caacttcgac agctggtaac
     6721 ggaagggttt ctctatcttg ataaccccaa gtataagcaa ttccaaaggc taccccaaga
     6781 ccgacaagca tagtcacaag gtattgtaat aaatggccat tcaagtaaag gagagttcct
     6841 ggtaagaccg taatcccaaa accagttcct gcaatgccaa agagcccagc gacccaacca
     6901 cctaaggcac caccaataag acctgaaaca aagacttttg gataacggag gttgacccca
     6961 aaaatagctg gttcagtaat ccctaaaaga gctgacaagg ttgatggaaa ggccagacct
     7021 tttagttttg ttgatttggt tttaacagca acagctaagg tagctccagc ttgagcagct
     7081 gttgctgctg ttaggtaagc attgaaagga tcttttccgg tattggcaat tagctgcgct
     7141 tctaggaagt taaagatatg gtgaatacca gtaacgacga tcaactgttg gataccacca
     7201 acaataagac cagcaatgcc aaacggcaaa tgcaagacgg cctgtgtccc agccagaaca
     7261 aggttttcaa gagaatggaa aactggtcca atcacaaata gtcccaaggt actcataata
     7321 gcaaacgtta aaaacggtgt gaccaataag tctaaagctt ctggaacctt tttgtgcaac
     7381 catttctcca acttagctcc taccagacca acaaagaagg caggtaaaac ggtaccttga
     7441 taaccaacaa caggaacaaa tccaaagaag gttagcggct taacatctcc accagaggca
     7501 actacccaag cattaggcaa ttcattggac accagcatca aacctaaaac aataccgata
     7561 ataggattac ccccaaagac cctaaaggct gaccaagcca ccaaagctgg caagtagaca
     7621 aaggctgtat ccgttagaat acgagtgtac atgagaaaat tttccccgta ctcatgcacc
     7681 ccaaataaat ccataatagc tggctgggtc accaaaccac gaacccccat aaagagaccc
     7741 gttgctacaa tagctggaat aatgggaaca aaaacatctc caaacgtacg aatcgcccgt
     7801 tggaagatat tgccttgttt gcctgcttct gccttttgct cactggttga tgatgttggt
     7861 aaaccaagag caacgacttc gtcataaatg ttattgacgg taccagttcc aaaaatcatt
     7921 tgatattgac cagaattaaa gaaagctcct ttaaccttgt caatagcttc tgctttttcc
     7981 ttatcaatct ttccttcatc atgaaccatc acgcgaaggc gagttgcaca gtgggcaaca
     8041 cttctcacat tttctcggcc acctaaagcc tcaatcactt cagttgcaat ctgacgatta
     8101 tccatgccta aaagtctcct ttttcatttg gtgttagtcc ctcatccatt ctgccagctt
     8161 tgtggctaag aatgtgcatg accaacgcta gtgaatccgc ttcacttctt gactatcatt
     8221 ttatcactaa tattttttat gtcaagcgtt taacatattt ttattttata tgctttaaat
     8281 tcttgatttt taaacagaaa acgtttagta tattcttgaa gacgactata aaacaaggag
     8341 attttgatgg atctaccaca agctattcgc taccgccctt acaaagagtg gagctcaaaa
     8401 gactaccaag ctattactga aaaaatggcc caatccccat ggcacagcca atttcatgtc
     8461 gaacctaaga caggattact caatgacccc aatgggtttt cttatttcaa cggtcactac
     8521 catctttttt atcaaaactg gccttatggg gctgcgcatg gtctgaaaca atgggtacac
     8581 atgacatcca cagacctcgt ccattttacg gaaacaagga gccgccttct gccagaccac
     8641 gctcacgata gtcatggggc ttactcaggt tctgcctatg ctattgatga caagctcttc
     8701 ctcttttaca ctggtaatgt tcgcgatgcc aattgggtca gaacaccatt acaggttggc
     8761 gcatggatgg ataaacaagg aaatatttcc aaaataccac aggttttgat tgaacagccc
     8821 gatgatgtta cagaacattt tcgggatcct cagctttttt cttatcaagg tcaattctat
     8881 gctattattg gcgcccaagg tttagatggc aaaggaaaaa tcaaacttta caaagctgtg
     8941 gacaatcatg tggacaactg gcgatttata gccgatttgg actttgacga ttctggcacg
     9001 gaatacatga ttgaatgccc caaccttgtg tttgtcgatg acaagcctgt tttgattttc
     9061 agcccacaag gactggctaa agctgattta gattatcaaa atatctaccc caatacctat
     9121 aaaatttttg aaagcttcaa tccagaaact ggccaattgc tgggtggggg ggcccttcaa
     9181 aatcttgact ttggttttga agcttatgcc actcaagcct ttagtagtcc agatggcagg
     9241 gttctagccg tgtcttggat tgggttacca gatattgatt atccgacaga tcgttatgac
     9301 tatcaaggag ctcttagttt agtgaaagaa ttaaggatta aagacggaat tctctatcaa
     9361 acacctgttt cagccctaca aaatcttcga gggccagcag aattatttca taataaaata
     9421 gacagttcta actgttatga attggaattg acgattccag gccaaaaaaa gcttgacctg
     9481 ctcctatttg ccgaccaaaa agggaatggc ctaagattga aggtggatac aacaaaagga
     9541 cagttaagca ttgaccgaag tcgggcaggg gttcaatatg cccaagacta tggtacggtg
     9601 cgctcttgcc aaatcccaca aggccatgtt acacttaatg tctatgtgga caattccatt
     9661 cttgaaatct ttattaacca agggcaaaaa gtcctaacta gtcgtgtttt cccaacccaa
     9721 ggacaaacag gtatccaagt agtagaagga caagcttttg gacattatta tgaaatgagg
     9781 tattaggcaa gtggtcgcaa aattaacaga cgtggcagcc ttggcgggtg tcagtccaac
     9841 aactgtttcg cgagtaatca accaaaaagg ctacctttcc caaaaaacag tgaataaggt
     9901 gaataaggcc atgcgtgaat tgggctacaa gcctaacaat ctcgctagaa gtttgcaagg
     9961 taaatcaacc caactcattg ggcttatctt ccccaacatt agcaatatct tttacgctga
    10021 attaattgaa catttggaaa ttgaattgtt taaacagggc tacaaaacta ttatttgcaa
    10081 tagtgaacat aatcccgtca aagaacgcga atacttagaa atgcttgcgg ccaatcaagt
    10141 ggacggcatt atttcttcca gtcataattt gggaattgag gattatgagc gtgtcgaagc
    10201 tcctattgta gcttttgacc gtaatctggc gccaaatatt ccggttattt cttcagataa
    10261 ttttgaaggt ggtaaattag ccgcacaaac tttgcaaaaa cacggttgtc aaaacattgt
    10321 catgattaca ggtaatgaca attctgattc gcccacaggg ttgcgccagc tagggtttaa
    10381 ctaccagctc aaacgaagtg cagaaatcat caaattacca aataacctat ctcctgttag
    10441 acgcgaaatg gaaattaaat ccattttggc aacccgaaaa cctgatggcc ttttcgtttc
    10501 tgatgattta actgctatcc tcattatgaa agtagccaaa caacttcaca ttactattcc
    10561 agaggatatg aaagtcatag gttatgatgg aacgacattt atccagcaat atgttcctca
    10621 attagcgacc attcgtcaac ctattgatga gattgctaaa ttgagtgtcg aaatactcat
    10681 caaaaaaatt aaaaaagaaa aaacaagtaa agactatatt ttaccgatta cgctacttcc
    10741 aggagcaagt atttaaacaa aacataacca atcactaatt actctaaaag acaaacaagc
    10801 atactctttc tagatgcgag agtatgcttg tttgtctttt aatattcgta cataaattga
    10861 aaaaatcgga tacaatccta agattgctcc gacttattat gatttatttg ctgaaggagc
    10921 ttccgagaca tattggctaa gtaagccatt aataaattta gcagaggttt catcagaata
    10981 ttttttagct acttcaatga tttcgtttag tgcaacacgg tctggtgttt cgtcaaaata
    11041 cttgatttca aaaagaccta gtcgtaataa cgttttgtct gttagtgtca agcgttctaa
    11101 cgaccaacct tttttcaaat gagttgatat caagttatcc aactcttctt tatgattatt
    11161 gacacccgtc acaagactta ataaaaaaat cggcagctcc aatacttgag cgtcctcccc
    11221 tgttacttta tcataaccat aggcaaactg agaagctgcc aataactcag ctcccatctc
    11281 tatgttaaat aaagcctgaa aggcacgttc acgcaaatct cttcttgaat tttgaaagct
    11341 gttagtcatc caagaaatcc tcatcaaaaa gtgacttcaa atctggtttt ggtgtctttt
    11401 ccgcaacaat accttcaaca tggatattaa ctgcagaaat aggcacttct gccatatcgt
    11461 acacagcaga tttgactgtt ttttgaatat tcatagatac agtcgggacc ttaactccat
    11521 attgcaagta aacataaata tctgctgtaa cacttccatc ttcttctgtc tggagataaa
    11581 cccctttacc aagacttgct ttgttaaagc tatctgccat ttttttgtta tgaagggaat
    11641 gaacgccctc aacttgtgtg gtagcaatcc cagtaataac ctcaagtact cgtggtgaaa
    11701 taacgatttc accgatatgt tcagttgtca tagcatttcc tttctcttct ttagactaac
    11761 ctgttctgac aaaggattaa gcacgagaaa cgtaagtacc ttctgcagtg ttgatgatta
    11821 gtttttgacc agcttcgata aagtctggaa cgttgaccac aagacctgtt tcaagagttg
    11881 caggtttacc agaacctgtg acagttgcac ctttgataga tggttgtgtt tcggcaacag
    11941 tcaattcaac ggttgttgga acagtcactc caatcacttc gcttccatag aattggattt
    12001 taacgtctga gttttcaaga atgtaaagca actcttgctc aacgttagca actggaattt
    12061 cgtactgatc gtaagtgtca gtattcatga agtaagcagt gtcatccatt ttatataggt
    12121 attgtgctgg aacagtttca atgatagctt gctcaaattt ttcatctgga cggtaagttg
    12181 tgtcaaaggt agaccctgta cgcacatcac gcaatttcat acgcataatt gtgtttcctt
    12241 tacctggttt atggtggcta gcttcaagga cacgaattaa tttcccttct gattcgaatg
    12301 tcataccggc tttaagctta cttgcttcaa tcattattat ttacctctta ctaaaatatt
    12361 atcacttcat tttaccacaa aagcgcccta ggctcaaatc ttttatgacc attcgcccaa
    12421 gctaaccatt aggtccttcc ttaccaccca aaatgatgtg aggcacgtcg tgttgaacgt
    12481 acgcaactcc ctttttctcc attagttcaa tcgcaaacgg gtgcggacga taatgtgctt
    12541 tgtaagtaat ctttttaatc ccagcctgca acagagcctt ggtacagttg atacagggaa
    12601 agtgcgttac ataaatttca gttccgtcag tcgaaatccc ttctttcgca cattgaatca
    12661 aagcattcat ttcagcatga actgtcctga tgcaatgtcc atcttccata taatggcccg
    12721 cttcattaca attatctgtg gcagaaacac caccattata acccgtggca ataatacgat
    12781 tatcctttac caaaacagct cccacaaagg cgcggtcaca ggtagagcgt ttggaaataa
    12841 gctctgcatt ggccataaag taatcttgcc atgataaacg atttgtcatt gcttcgtcct
    12901 tctaatcctt ccctaagata atttacaaca caatcagttc ctttggtgcc aaggtcaata
    12961 cttgacagcc agtttctgtg atgaccaagt catcttcaat acggacgcca tatttattat
    13021 ccagatagat tcctggctcg tctgttacca ccattcctgc ttgaagaagt tgctcagatt
    13081 taccaaaaaa cggattctca tggatgtcaa gcccgatacc atgaccaatg ccatgtgtga
    13141 agtgactacc ataaccagcc tcagtgatga gttggcgcgg aataccgtca aaatcgctat
    13201 aagtcatacc agcgctagct ttatcaatga gagccttatt agcagcaaga acaagagcat
    13261 aaatctcacg ttcttcatca gtaacttggc caatatgaat ggtcctcgtc atatcactaa
    13321 catagtgatt gtagtaacac ccaaagtcca tggtcaagct ctctttattc tggataacct
    13381 tgtcactggc gcgtccatga ggcatggcag agcgatagcc tgaagctaca atgatatcaa
    13441 atgatgtgcc gctggcacca tactgacgca tacgaaaatc taaaaaatta gccaggtcac
    13501 gttcagtggt tgtccctggt ttaataaaat caagagcatc ttcaaatgct ttgtctgaga
    13561 tcgagcacgc tttagcaatg gtatcaattt cagaagtatc cttaataaaa cgtaaatgct
    13621 ccacaaaacc tgactgagca agcaaggtta ttcctgacag ttctgcttgc atggcctgat
    13681 aaaaagaaaa cgatacctgg tcctcaaaac caaggcaatc tatttgatca gcctctaaca
    13741 attctgccac aaccttaagc ggcgtgcggc tttcgataat atcaaatccc tcaacactag
    13801 ctttagcaag caaggtataa cgtgaatctg tgatcaaaac acgacgtttg gccgttatca
    13861 aaacagttgc tgcagttcca gaaaaacctg tcaagtaata actattggtt aaatgggtga
    13921 ctagaagagc ctctagcccc ttctctgcca tctgccttag gcagtgacct aatcgttgct
    13981 ctaaaaatcc tgacatcgcg aactcctttg gtaacttttg agaatattgt agcatgaaaa
    14041 aactacttta agtttaccat gatttgagag catttaaaag attaaaaagg agctcaaacg
    14101 aactcctaat cctattgttg tagttttact ttcaaataat gaccagtata gctttcttta
    14161 acttgagcta cctcttctgg tgtccccgtg gcaacaatct gaccacctcc gacaccacct
    14221 tctggtccta agtcaataat atggtcagca gatttgataa cgtccaaatt atgctcaatc
    14281 acaagaaccg tattaccatc gtctacaaag cgttccaaaa cttttaatag ccttgcaata
    14341 tcatctgtgt gtaaaccagt cgttggctca tctaggatat acaagctctt acctgtcgag
    14401 cgtttgtgca attcactagc taatttcatc cgctgcgctt ctcctccaga tagagtagta
    14461 gctggttgac ccaaggtcac ataacccaaa ccaacatcct taatggtttg aatttttcgg
    14521 gcaattttag ggatagcaga gaaaaatacc aaggcatcat cgacagtcat gtcaagtact
    14581 tcagcaatat ttttttcttt ataatgcacc tcaagcgtct cagaattata ccgtctgccg
    14641 tggcaaacct cacaaggcac ataaacatct ggcaaaaagt gcatttcgat tttgataatc
    14701 ccatcacctg aacaagcctc acaacgccca cctttgacat taaaagagaa acggcctttt
    14761 ttgtagccac gaatcttagc ttcgttggtt tgggcaaaca agtctcgaat atcatcaaag
    14821 acacccgtat aggtggctgg atttgaccgt ggcgtccttc caatcggact ttggtcaatg
    14881 tcaatcaagc gttcaatatg ttcaatacca gaaatagagt gatatttacc aggcttatca
    14941 gcattacggt ttaatttttg ggcgacagcc tttttcaaaa tactattaac caaggttgat
    15001 ttccctgagc ctgatacccc tgtgaccgca ataaatttac ccagtgggaa tctcacgtcg
    15061 aggctttgaa gattattctg cgctgctcct tttatttcaa tgaatcgccc attaccagaa
    15121 cgacgctcta atggcactgg aatgaatttt ttaccagaca aatattgacc cgtgatggat
    15181 tttttattct tagccacttg tttcggcgtt ccagaggcaa taatttctcc accgaactca
    15241 cctgccccag gccccacatc aatcagccag tctgcctgca tcatggtgtc ttcatcgtgt
    15301 tctaccacaa ttaaggtatt acccaaatca cgcatttttt tcaggctttc gatcaaacgg
    15361 tcattgtctc tttggtgcaa tccaatagaa ggctcatcca agatatagat cacccctgat
    15421 aagttagaac caatttgtgt cgctaacctg attcgttgac tttcaccacc tgataaagtc
    15481 ccagcagccc gtgacaaggt cagataattc agtcccacat tatttaagaa ggtcaggcgg
    15541 tcatgaattt ctttgacaat cggtttagca atggtacttt cattttctgt gagttccaat
    15601 tcttccaaca actgcaagtg gtcggcaatc gataattctg aaatttgacc aatatgaggt
    15661 ccttcttcgc cacccacatg cacacacaag gcttggtcat tcaaacggta accatgacaa
    15721 gtcgcacagg tcaattcgtt catataacca cgcatcacat tgcgcgtata atcactatta
    15781 gtttcgtgat aacgacgatt aacattagtc acaacacctt caaaaggaat atcaatgtta
    15841 cgctcaccac caaaatcatt cacatagtga aaatgaaatt ctctatctcc tgaaccataa
    15901 agcactaaat ctctttcttc ctcagtcaag gcctcaaaag gtgtatccat atccacccca
    15961 aaacttgcca tagcttgctc aagcatggtt gggtaataat tggaggaaat aggattccaa
    16021 ggcgccaaag ctccctctct caaagactta cttgggtcag gaacaaccaa gtccaaatcc
    16081 acttccaact taatgcctag cccatcacag gtcggacaag agccgaacgg cgcattaaaa
    16141 gagaagagtc ttggttctaa ttcaggaacc gtaaacccac agacaggaca ggaataatgc
    16201 tcagaaaaga gcagttcatt gccatccata gtgtcaatca taaggtagcc atctcccagc
    16261 cttaaagctg cttcaaccga atcaaataaa cggctacgaa taccatcctt attaaccaga
    16321 cggtcaataa ccacctcgat gttgtgcatc ttacttttgg acagctccgg cacttctgtc
    16381 acgtcaaaaa tatccccgtc aacccgcaca cggacataac catctttttg aattttctca
    16441 aaaatggttt tgtgttgacc ttttttccgg cgaacaatag gcgctaaaat ctgcatgcgt
    16501 gtccgttcag ggagggctaa gacctgctct acaatttgtt ctgcagaaga tgctgtaatg
    16561 gccccatgcc cattaataca gtaaggtgtg cccacacgcg catatagtaa acgtaaataa
    16621 tcattgattt ccgtaaccgt accaaccgtt gagcgggggt tcttactggt agttttttga
    16681 tcaatggaaa tagcagggct aagaccgtca atggaatcca catctggttt ttccatatta
    16741 cccaagaact gtctagcata agctgataaa ctttctacgt aacgacgttg cccttccgca
    16801 taaatagtat caaaggctag actagatttt cctgaccctg ataaccctgt cacgacaact
    16861 aatttgtctc gtggaatttc cacgtcaata ttttttaaat tgtgggctct tgccccatga
    16921 attattattt tattttgcat cttgaatgtg cctaaaggca ccctcctcat cgagctttct
    16981 tatattataa caaaaatttc tgataaccta ttgccccaaa ctttgcgaat tttggtataa
    17041 tagataagta ttgagaattt ttcagaaaat ccttgagaag gctttattat caaggtttga
    17101 tgtcaatctc taccagtcaa tcatcaaaaa ggagggcttg tcatgaaaca aatgtttctt
    17161 tcatcggcaa ttgaattcaa agaaatagaa acattcgagc caggagcttg gattaaactg
    17221 gttaaccctt cccaagaaga gtctatgaag atcgccgatc aatttaacat tgatatttct
    17281 gactt
//
LOCUS       DAUVUY010000033        17195 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.033, whole genome shotgun sequence.
ACCESSION   DAUVUY010000033 DAUVUY010000000
VERSION     DAUVUY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17195
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.033"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..343)
                     /locus_tag="VOX58_000966"
     CDS             complement(<1..343)
                     /locus_tag="VOX58_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HES5878199.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVNVNLR"
     gene            complement(340..1191)
                     /locus_tag="VOX58_000967"
     CDS             complement(340..1191)
                     /locus_tag="VOX58_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HES5878200.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKK
                     ILGGKSK"
     gene            1298..2641
                     /locus_tag="VOX58_000968"
     CDS             1298..2641
                     /locus_tag="VOX58_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HES5878201.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     GRQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFICLNCGFKRPQLDYRLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIMEAIEKQESKHAYILATYTAMLEFRSLLADRHVVEKEMN"
     gene            2641..3432
                     /locus_tag="VOX58_000969"
     CDS             2641..3432
                     /locus_tag="VOX58_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HES5878202.1"
                     /translation="MTYTSLKSPENTDYLYDLNIAHLYGNLMNTYGDNGNVLMLKYVA
                     EKLGARVTVDIVSINDTFEQDDYDIVFFGGGQDYEQSIVAKDLPSKKAALADYIANNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTSLKKKYGSAISLPSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(3545..4534)
                     /locus_tag="VOX58_000970"
     CDS             complement(3545..4534)
                     /locus_tag="VOX58_000970"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HES5878203.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFECLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            4767..7184
                     /gene="hylA"
                     /locus_tag="VOX58_000971"
     CDS             4767..7184
                     /gene="hylA"
                     /locus_tag="VOX58_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HES5878204.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQMLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASANITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSSIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTSVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYHAYVNNQPVDLNNQLVDFTNTKSIFLESDDSAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATMFSNHNLSHQGFYSFPH
                     PVKQNQQ"
     gene            complement(7857..9620)
                     /gene="lpdA"
                     /locus_tag="VOX58_000972"
     CDS             complement(7857..9620)
                     /gene="lpdA"
                     /locus_tag="VOX58_000972"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HES5878205.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAI
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(9951..11360)
                     /locus_tag="VOX58_000973"
     CDS             complement(9951..11360)
                     /locus_tag="VOX58_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HES5878206.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
                     RVHKADVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLAEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            complement(11545..12543)
                     /locus_tag="VOX58_000974"
     CDS             complement(11545..12543)
                     /locus_tag="VOX58_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HES5878207.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            complement(12602..13570)
                     /locus_tag="VOX58_000975"
     CDS             complement(12602..13570)
                     /locus_tag="VOX58_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HES5878208.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            complement(13856..15763)
                     /locus_tag="VOX58_000976"
     CDS             complement(13856..15763)
                     /locus_tag="VOX58_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HES5878209.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDKTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMMDYDLLAERFRQQGGFTYEAEIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQETLME
                     EWETIAEQIES"
     gene            complement(15809..16135)
                     /locus_tag="VOX58_000977"
     CDS             complement(15809..16135)
                     /locus_tag="VOX58_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878210.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            complement(16165..16950)
                     /locus_tag="VOX58_000978"
     CDS             complement(16165..16950)
                     /locus_tag="VOX58_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HES5878211.1"
                     /translation="MASIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGN
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            complement(17083..>17195)
                     /locus_tag="VOX58_000979"
     CDS             complement(17083..>17195)
                     /locus_tag="VOX58_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878212.1"
                     /translation="FDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
BASE COUNT         5226 a         3581 c         3046 g         5342 t
ORIGIN      
        1 ctcttaggtt gacatttact gtacctggat gactatgtgt tagatccgcc acaatcttaa
       61 aattacgtgt atcattgttc acttcagaat ctaatttgat acgattagtg gaggttagat
      121 aaacttccgc accataagaa tacccactga taaaatattt gtcactatca tacttcatat
      181 caattggtac atctttaagg ctatgcgtat aggtttcaat cgggctataa atttgcgagc
      241 tagaattatt atgattgctc gatgctgcag ttaaaaaaag gagaatcgca aaaaagactg
      301 ataccattcc taaccaagga cgactattta aaaatcgttt catttagatt tccctcctag
      361 aattttctta taccatggta aactaacatt tgaatcactc atcaggtagg tcctaagtac
      421 cgtttcaaat tcatctgtgc ttaaatcatg taaaaattgg cctttgcgag ttactgaaat
      481 agctcctgtt tcttctgaaa caatgatagt tagtgcatca gagttttcag ataatccaat
      541 agccgctcta tgacgggtcc caaattcttt ggaaattgcc tttgattctg acaaaggcaa
      601 gtaagcacag gctgccacaa tcttattctg accaacgata acagctccat catgaagcgg
      661 cgtattcgga ataaaaatat tgatgagtag ttgacttgaa atgtcggcat tcagcggaat
      721 tcctgtcgca atatattctt gtaaggtttg agtctgttca atagcaatca atgcacctat
      781 cttgcgtgga cccatatagg ccacagattt aatcaaagca tcgaccaaag cagattcact
      841 gctgacgtac tgtttttgta aaaaaacttg cgtagaacga ccaaatttct ccagtcctgc
      901 ccgaatttca ggggtaaaaa tgactactcc tgcaattaca ccataagtaa tgacttggtt
      961 cattaagtat gtgattgtcg taaagccaat ccactctgca ataaaacgaa gaaccaaaaa
     1021 gaaaatgacc ccttgaacta aagacatgat tttagttcca gtcaatgctt taataaaacg
     1081 ataaatcaaa taggcaacaa ttagaatatc taaaagatgc actgccagca gccaaggatc
     1141 cgcaaataag cttaataaaa atttaatatc gatactagat aaattattca tacaactctc
     1201 atttctatat caaaaaagtt attagctata ttatatcata tcatgatcaa agatttttcc
     1261 ctatctaaaa tcaataaact tatgataaaa tataagcatg aaaatgaaga cattactcgg
     1321 cattattgct ggcaaagctg ctcaatccat tttaacaaaa ctagggcgag gatctactta
     1381 tcctggtcga ttggctctag cttgtaataa agatatttta aaggatttgt ctaaggacta
     1441 tgatattgtg gtcgtcactg gtactaatgg taaaacgtta acaactgctc ttactgtcgg
     1501 tattttgaaa gaggcatttg gcgagataat cacaaatcca agtggtgcca atatgattac
     1561 tggaattact tctacttttt tagctgctaa aaaagggaaa tctggaaggc aaattgctgt
     1621 tttagaaatt gacgaagcta gtcttcctag aattaccact tacttaaaac ctagtctttt
     1681 tgtttatact aatattttta gggatcagat ggaccgctac ggagagattt atactactta
     1741 tcagatgatt gtagatggtg ctcgaaatgc tccaaaagca actattctag ccaatggaga
     1801 tagtcctatt ttctcttcta aggacatagt taatccagtt cagtattatg gttttgatac
     1861 tgctaagcat gctccgcagc tagctcatta caacacagaa gggattttat gtccaaaatg
     1921 cgaacatatt cttcagtatc gtctcaatac ttatgcgaat ctaggagact ttatttgttt
     1981 aaattgtggc ttcaagagac cacagttgga ttatcgatta actgaactca ccgctattac
     2041 tcaccaaagt tctgaatttg ttattgatgg acaaaactac aagataaatg ttggtggtct
     2101 ttataatatt tacaatgcgc ttgctgcggt ttctgttgct gaatttttcg gtgtttctcc
     2161 cgagaaaatc aaggcgggct ttaacaaaag taaggcggtt tttggaagac aagaaacttt
     2221 tactattggt gataaatcat gcacccttat ccttatcaag aatcctgttg gagccagtca
     2281 agcgcttgaa atgattcagc tagctgatta cccatttagc ttatctgtgc ttttaaatgc
     2341 taattacgct gacggcattg ataccagctg gatttgggat gctaattttg aactcattac
     2401 ccaaatgccc attacagaaa ttaatgctgg cggcgtccgt cactctgaaa ttgccagaag
     2461 acttagagtc acaggatttg acgacactaa gataaaacaa gctgaaaagc tcgagcaaat
     2521 catggaagct attgaaaaac aagaaagcaa acatgcttat attttagcaa cctatactgc
     2581 aatgttagaa ttccgtagct tgctagccga ccgccatgtt gttgaaaagg agatgaacta
     2641 atgacttaca cttcactaaa atctccagaa aacactgact acctctatga cctaaacatt
     2701 gctcatctct acggaaacct gatgaatacc tacggtgaca atgggaatgt cttgatgctc
     2761 aagtatgtcg ctgaaaaact tggagctagg gttactgttg atattgtatc tatcaatgat
     2821 acctttgaac aagacgatta cgatattgtt ttctttggtg gtggtcaaga ttatgaacaa
     2881 agcattgttg ccaaggattt gcctagcaaa aaagctgcct tagcagatta cattgctaat
     2941 aataaagtgg tgcttgccat ctgtggtggc ttccaattac tagggcaata ctatgtccaa
     3001 gctaatgggg ttaaaattga tggacttgga attatgggac actacaccct taatcagcac
     3061 caaaatcgct ttattggaga tattaaaatt cataatgacg aatttaacga aacctattat
     3121 ggctttgaaa atcatcaagg acgtaccttc ttatctggtg acgaaaagcc acttggacga
     3181 gtggtttatg gcaacggtaa taataaagaa gaccaaactg aaggtgttca ctataaaaac
     3241 gtctatggta gttatttcca cggtcctatc ttatcacgca atgttaatct tgcttaccgc
     3301 ttagtaacaa catctcttaa aaaaaagtat ggatcagcta tttcactacc tagctatgat
     3361 gatatactga aacaagaaat cactgaagaa tatgccgacc ttaaaagcaa agcttccttt
     3421 aataaagttt aagttcttct ttcatgtcat caaacaaaaa ctcctaagca ggacacgata
     3481 gatagttcgc ttaggagttt tatagttttt tcttctgaca gagcttctta agtgattaaa
     3541 accactaaga aacaatagct ttagcaactt cttcaacggt catgcgtgag aagtactggg
     3601 tcgtatcaat cgtttttaaa cattcaaaaa catcacggta ctcatattta caaccaatta
     3661 ataagttctc aatgtcttgc acatctttga tcccaaagaa atcaccatag attttaaggt
     3721 ttttaatgat agaattttca acatttgcaa aggttgaaat tttacctgca gggtaacgaa
     3781 cattgcgttc aatcgtatat tctggcgctt tgccgtaggt ccaatcccaa ctaccaaatt
     3841 gctctttggc agattgttca atcttagcca actcatcctc tgacaagaca tattctgtca
     3901 tgtctggata cgtttctttc atcttggtca agattttatc tgaaaattcc tcaacggtaa
     3961 ttttttctgg taattcattt aaaatattgg tcacgcgagc acgaacagac ttcactcctt
     4021 tagactcaat cttatcctta cttaccttaa gagcatcgcc aagaacggtc atatccacat
     4081 caaacaaaag gcaaccatgg tgcatcatgc gacctttgta gtaagcttga gcattaccac
     4141 agattttttt gccatcaatt tcaatatcat tacggcctgt aaagttggct gttacaccca
     4201 aatcagctaa agtcgcaatc acaggttgtg agaaggtttt gaaatcaaat gcgccttccg
     4261 ctgttttatt tgaaatgata gtgtagttca agttattcaa atcatgataa accgcaccac
     4321 caccagataa acgtcgcaca acatgaatac catgttcatc aatgtattct ttattgattt
     4381 cttgaatggt gttttgatgt ttgccaataa taatagctgg ctcattgatc caaaggataa
     4441 acaattcgtc ttcttccact agttctctaa aagcataggc ttccaaagcg atgttaaagg
     4501 caggattgtg acttttgtta acgatgtatt tcataggaga aaaaatcctt tcaaaaatgt
     4561 aatctacctt tctagtataa tgaaaacaaa tataaaagac aagtgtccta ttctgtatga
     4621 catcttcagt acctacactt attagtgcac aaaaactcag ccattattta tgtaagggtt
     4681 tataaaaata ctttaacgtg gtatattttt acttagtaaa ccaatttact tttattatct
     4741 catttttata taggaggaat ttatcagtga atacttattt ttgcacacac cataaacaat
     4801 tactacttta ttcaaaccta ttccttagct ttgctatgat gggccaagga actgccattt
     4861 atgccgatac actgacttca aattcagaac ctaataatac ttactttcaa acgcaaatgc
     4921 tcactactac agatagcgaa aaaaaggtag tacagccaca acaaaaagac tactatactg
     4981 aattgttaga ccaatggaac agtattatcg caggcaacga tgcttatgat aaaaccaatc
     5041 ctgacatggt cacttttcat aataaagctg aaaaggatgc tcaaaacatt attaaaagct
     5101 atcaagggcc tgaccacgaa aatagaactt acctttggga acatgcaaag gattattccg
     5161 cttctgctaa tatcacgaaa acttaccgca atattgaaaa aatagcaaaa cagatcacta
     5221 atcctgagtc atgctattat caagatagta aagctattgc tattgtaaaa gacggtatgg
     5281 ccttcatgta tgaacacgct tataatctag atcgtgaaaa tcatcaaaca actggaaaag
     5341 aaaacaaaga aaattggtgg gtttatgaaa ttggaactcc tcgtgctatt aataatacct
     5401 tatccttgat gtatccttat tttactcaag aagaaatcct taaatacaca gctccaatcg
     5461 aaaaatttgt gcctgaccct actcgtttta gggttcgcgc tgccaatttt tcaccttttg
     5521 aagccaatag cggaaattta attgatatgg ggcgtgttaa actcatttcc ggtattcttc
     5581 gtaaagatga tctcgaaatt agtgatacaa tcaaagcaat tgagaaagtt ttcacgctag
     5641 ttgacgaagg aaatggtttt taccaagacg gttctttaat tgatcacgtg gttactaacg
     5701 ctcaaagtcc actttataaa aaaggcattg cttacactgg agcttacggt aatgttctta
     5761 tagatggctt atcgcaatta attcctatta ttcaaaaaac aaagtcttct atagaagcgg
     5821 ataaaatggc tactatctat cattggatta accattcttt tttccctatc atcgttcgtg
     5881 gagaaatgat ggatatgact cgagggcgtt ctatcagtcg ttttaatgct caatctcatg
     5941 ttgctggcat tgaagcactt cgtgctattt tacgtattgc tgacatgtct gaagagcctc
     6001 accgtttggc acttaaaaca cgtataaaaa cactcgtcac acaagggaat gctttttaca
     6061 atgtctatga taatttgaaa acctatcacg atatcaaact tatgaaagaa ctgctaagtg
     6121 atacttctgt tccagtccaa aaacttgata gttacgtagc tagtttcaat agtatggata
     6181 aattggcact atataataat aaacacgatt ttgcttttgg cctatcaatg ttttcgaatc
     6241 gaactcaaaa ttatgaagct atgaataatg aaaatcttca tggctggttt acttctgatg
     6301 gaatgtttta cctatacaat aacgatttag gacactacag tgaaaactat tgggcaactg
     6361 taaatcccta ccgcttacct ggaaccacag aaactgagca aaaaccacta gagggaactc
     6421 ctgagaatat taaaacgaac tatcaacaag ttggcatgac tagtctctct gatgacgctt
     6481 ttgttgcaag taaaaaactt aataacacaa gtgctctagc tgctatgacc ttcactaatt
     6541 ggaataaaag cctcactctc aataaagggt ggtttatctt aggaaacaaa ataatctttg
     6601 ttggtagcaa tatcaaaaac caatcatctc acaaggcgta tacaactatt gaacaacgaa
     6661 aagaaaatca aaagcaccct taccatgctt atgttaacaa tcaacccgtt gacttgaata
     6721 atcagctagt tgattttaca aacactaaaa gtattttcct tgaaagtgat gattctgctc
     6781 aaaatattgg ttactacttc ttcaagccaa caacacttag cataagtaag gcacttcaaa
     6841 cagggaaatg gcaaaacata aaagctgatg acaaatcacc agaagccatc aaagaagttt
     6901 caaatacctt tatcactatc atgcaaaacc atactcaaga tggcgatcgt tatgcctata
     6961 tgatgcttcc aaatatgact cgtcaagaat ttgaaaccta tattagcaag cttgatatcg
     7021 acttgttaga aaacaatgac aaactggccg ctgtctacga tcatgatagt caacagatgc
     7081 acgtcattca ctatgaaaaa aaagcaacga tgttttcaaa tcataatctt tctcatcaag
     7141 gcttttatag ttttcctcat cctgtcaagc aaaatcaaca ataaaagctt gctcatcaag
     7201 gaattgcagc taaaaacaat gctcttaact ctcataaaat tccccacaaa agacaacgac
     7261 gcttaccgca tactgggtat cagtcctcct ctttagaatt cttaggtgga gctttagttg
     7321 cttcttttaa ccatatcaca aaaccctttc gtaagaaaga cttaaggatt tagctgttat
     7381 tggaaagtaa gggttaacaa tgaaacgtta ttaaagacaa aaaactggaa actgcactga
     7441 gccccaaaag ttggctactc tatttgaagt aaaggattta gttctgtatt gtagaggact
     7501 aagtcctttt cgttttattt tgatacggtt attggtgcaa taatcgatga taatagtagg
     7561 tagaacgagt cactttagcc gtccttaaaa gaaaatctaa tcagagctcg tctttgtcca
     7621 ttctttataa tggttttttg tcttcttctt ttcagagatg cttctttcaa acggagttct
     7681 ctcgtttttt tagcgctaaa tgtctagctt atcttcatag cttaatgtca tacaaaaaca
     7741 ccccaaacgt tagggtttta tatctaacgt ttggggtgca gttcatagtt gtgctagtct
     7801 tttttgtgat ctgttatgtg atgatatttt tcattagtta ataagacaaa gacgttttac
     7861 ttacgttttg gtgggttatg gatagcttcg ccaagcacat ctgcaaaggc ttcgtacatc
     7921 acttcagaga aggttgggtg tccatgaatt gataataaca attcatcaac ggttaattca
     7981 gattccataa tagtagctgc ttcgttaatc atctcagctg cagctggacc aataatatga
     8041 acacctaaga tttcgtggta tttggcatca gcaataactt taacaaaacc atgggcttca
     8101 tttgaagcaa tcgcacgtcc attaccagta aagctgtttt taccaataag cacatcacca
     8161 tattgttcac gcgcttgttc ttcagttaaa ccaaccattg ccacttcagg gtgtgtgtaa
     8221 acggctgctg gagtgtattt aaggttagct ttacgagttg tgttgccatg tatcgcattt
     8281 tctgcagcca cttcacccat acggtaagca gcgtgagcaa gcattttcgt tccgttaacg
     8341 tcacctggcg catagatacc tggaattgat gtctcttggt agtcattaac tttgatacgg
     8401 ttacgatcca tttcaaggtt aagattttcc agaccgttca tttgtggtac acgaccgata
     8461 gaaagaagag ctttttcagc gacaacttct tcaccattgt taagtttcaa ggtcaattgg
     8521 ttatttgctt caacaatttc agagacacca acagatgttt tgatcttcat gcctttctta
     8581 gaaaggattt tttgaagttc aagagagact tccttatcca tagctgggat aatgcggtca
     8641 gccatttcaa taacggtaac atccacaccg taagatgccc aaacaagtcc aagctcgata
     8701 ccaacaacac caccgcccat aactgctagt gattttggca tttcacggag gtcaaggata
     8761 tcgtcagacg ttaagacaag tttagagtca attcctggga tattgatacg tgatacttta
     8821 gaacctgtag caaggataac gttgcgaccc ttaatggttt gtgacccaat ggttacagtc
     8881 ttgtcagggt taacttgacc aagcccatta aagatagtga ctttattagc ttttagaaga
     8941 ccttgaacgc cgcctgttaa ggttttaaca acggtgtttt taaagtcaac tgttttgtcc
     9001 atgtcaatgg tatagttagt tgaggcaagg ttaatcccac gtcctgctgc aatcttaata
     9061 ccatcaagga tttcagcatt tttaaggtaa gttttggttg ggatacaccc tacgttcaag
     9121 caagttccac caaattcaga tttctcaacg atggcaattt taccaccaag ttgagcacca
     9181 cgaatagctg cataataacc agcagggcca ccaccaacaa cgatgatgtc atactcatca
     9241 tcagcaagtg cagcttttgg tgcagctgaa gcagcttgag ctggtgcttt tggcacttca
     9301 agtccagcag cttctaaatc ttctgttgta cgagcaacat tcacatctga agcagctggg
     9361 ctagaaacct caacagattc accttcagca ccgatatatc cgataacttc tgttacaggt
     9421 actgtttcac ctgcttgacg tgtaatttta agaagaacac ctgagtcttc tgcttcaagt
     9481 tccatattgg ttttatctga catgatttca agaagaatat cgccttcatt gacagtatca
     9541 ccttcttgtt ttttccactc gatgatttca ccttcttgca tgtcaacacc gagttttggc
     9601 ataataattt caacagccat aatttaagat accaagagca aggagaaagc gattgaattt
     9661 ttgcggtaag tcagaaatct ttgatttcgt tgacgcaccc acgtcaggac gactaggact
     9721 ttccgaaaca aaaattgagg aaagttcaga cgtctacgac gctaagatta gccaactctt
     9781 accattttta tccgagcttt tcgctcggat tcaattgctt atcactcatt tgcatgagat
     9841 ctcctcgttc tacatccttt ctatattttt gagaaaaggg cacccaccaa gtctaaactt
     9901 agcaaaatcc cttttattat tgaatgcttt tcttgtttaa aactgattga tcagattaac
     9961 aattcaaatg gattttccat caatttcttg agatcaacca tgaattttgc accgttcata
    10021 ccatcaacta aacggtggtc gatggttagg cacattgcca taattggacg ggcaacaatc
    10081 tcaccatcca caaccgttgg agttgggata gttgctccaa caccaagaat tgctgagttt
    10141 ggttggttaa taattgggtt aaatgtcttc gtaccaaaca ttcccaagtt tgtgatagag
    10201 aaggttgatc cagacatttc agctgctttc aacttaccag tttgcgcttt tttaatcaca
    10261 tcttttgaag caagcacaaa atctgacaag ctcatcttgt cagccccatg gataacagga
    10321 acgataagac catcatcaag acctacggca ataccaaggt tcacaaagcg gtgtaattca
    10381 atatcattag catcattgat gagggaagca ttcatgtact catgctcagg tttcatcaag
    10441 gttttaacca ctgccatacc aatcaagtct gtgaagctga ctttaaggcc tgttttagcc
    10501 atgattgggt caattaactt cttacgaagg gcaatcatct ctgtcatgtc aatatcgtag
    10561 ttaagcgtaa aggtaggagc agtaaggtaa gagtttgtca tacctttaga gattgcctta
    10621 cgcatagctg acattggctt gtgttccaca ccttctgcaa gatcaacaac tttttcttct
    10681 ttagctgctg gggcttttgc ttcagctggt ttagccgctt caagaatagc catgatatct
    10741 tctttcatga ccttaccgtt aaagccagta cctgatacgg ttgccaaatc aatacctttg
    10801 tctgctgcaa ttttacgagc aagtggtgaa gctttcggtt gagcaccttt aaagttttca
    10861 acgtctgctt tatggacacg tccttttggt cctgttcctg gaacttggcc aaggtcaatg
    10921 cccatttctg ctgctgcctt acgtgcagct ggagtagcac gaacttttcc gccatttcct
    10981 tgcggaatag ctgtcgctgc cacttgagga gctggacttg ccacattttc ttttggagca
    11041 actgctggtc cagcgtctgc tgaagcagga actggaattt ctgttgtttt ttcacttgaa
    11101 gcaatagtat ctactgattc accctctgca ccaatgtaac cgataacttc agttacaggt
    11161 acagtatcac cagctggacg gacaattttc aatagcacac ctgagtcttc tgcctcaagt
    11221 tccatattgg ttttgtctga catgatttca agaaggatat caccttcatt gacggtatca
    11281 ccttcttgtt ttttccactc gatgatctcg ccttcttgca tgtcaacgcc cagctttggc
    11341 ataataattt caaatgccat ttctacatcc tttcacgatt tgataagcgc catcaacatc
    11401 cccaccttgc tacgaaaaca tacgaaaggt gaggtgccgc gacacgggat aagacaactc
    11461 agttcttcta aaaactttca aaatagtttt tagctgagac gttactgata aacatagcct
    11521 ttatcagacc acttgcctta tcttctagtt gcctttgttt gccattttca caatggcagc
    11581 tttgattttc tcaacgtctg gtaagatcgc ttgttcaagt acacgtgcat aaggtactgg
    11641 aacatcttca cttgcaagac gaacgattgg gtgatcaagg taatcaaagg cttcactttc
    11701 agtaatcatg gttgcaattt ccccaatgaa tccaccggtc ttgtaggcat cgttgacaag
    11761 catcaattta cctgttttct taacagattc aataatcaac tctttatcaa gtgggatgag
    11821 ggtacgtggg tcaacaactt caacgttaat accatccgct gcaacttctt cagccgcttg
    11881 aaggacacgc tccaacatac gaccatatga cacaattgtc aaatcagttc cttcacgttt
    11941 gatgtcacct ttgccaagtg ggatatagaa gtcaggatct tggttaactt cttctttttt
    12001 accataaagc gcttttggtt ccatgaaaag aacaatgtta ttatcacgga tagctgattt
    12061 taataagcct ttagcatcgt tggcattacc aggagcaaca actttgattc ctggaatgtg
    12121 agtcaaccat gcttcaagag attgtgagtg ttgcgcagcg gaaccaatac cagaacctga
    12181 tgccacacgg aaggttacag gggtaataag gcctccaccg aacatgtaat tatttttagc
    12241 accattattc acgatagcgt ccatcatgat ggtaaggaaa tccataaagg taacgtcgac
    12301 aattggacgt agtcctgtaa tagctgcacc aatagccgca ccagagatag cagcttctga
    12361 aattggggta tctttaacac gtttaggacc aaattcttca atcattccaa cagaagtccc
    12421 gaagtctcct ccgtaaaccc ctacgtcttc tcccataagg aaaatgtttt cgtccttgcg
    12481 catttcctca gtcatagcaa ggttgactgc ttcacgcaaa gccattaatt ttgtttctga
    12541 catcattttt atttctccat ttattattca aatacgattg cttcttactg acaaggtgat
    12601 cttagtcaac ccaaacgtct tcgaaggcta ctgaaagttc tggatctgga ctgttttgtg
    12661 caaattcata agcatcgtca acttcttttt tgacttgtgc ttgaatagca tccaattcgt
    12721 catcagtggc aatgccttcg cttgtcagat aagtacgata tttgatcatt ggatcttttt
    12781 ctttccactc atccacttct tctttagtac ggtatttacc agcatctgca gtcgagtgac
    12841 caaaccaacg gtatgattct acctcaacaa tagctggacc atttccacca cgaacatgtt
    12901 caacggcttt gcccattgtt tcatagacag ccatcacgtc attgccatct tcacagtaga
    12961 aaccaggaat tccgtaagct tctgcacgtg tgtataaatg tggtgtattt gttgcattat
    13021 tgatgctcat tgaaattcca taacggttgt tgatgatgaa gaaaataaca ggaagtttcc
    13081 atgttgcagc catattaaca gactcgtgga atgatccttc gttagtagcg ccatcacctg
    13141 agaaagcaac agctatattg ttagttcctt tgtattgttg ggtgagggca gcaccaactg
    13201 caagagcgta accaccacca acgataccat tggtaccata gtttcctttt tcaaaatcag
    13261 ccaagtgcat tgagccacca cgccctttag aaacaccagt cgctttacca gcaagctcag
    13321 ccatcatttt attaaggtcc atgtctttag cgattgattg tccatgtcca cggtgatttg
    13381 aaaaaataat gtcatcgtaa gacaagtgag ccaccgctcc aacattggct gcttcttcac
    13441 caactgagaa gtgagtcatg ccttgtacaa atccacgacg gacgagttta ttaatacgtg
    13501 aatcaaattc acgaatacgt tccattttaa ggaacatatc caaatgttgt tctttagaaa
    13561 ctgttaccat ttcggcctcc atattatcat attacctttt tatgataaaa ctttttttca
    13621 caatttgcaa ataattaaat cgctttctac cttaccttat ttggtttttt agaaatgcaa
    13681 tagtttgtga aattacaaac aaaactgtac taaaaaattt aaaaatacta tataacagtt
    13741 tcacgtttta ccgaccttga cataaaaaaa cacatcattt taaaacctct ttcacacctt
    13801 caaacaagta acaaagcgat aaaaataagc ttagcaaaac tgctaaagct tatttttatg
    13861 attctatttg ttcagcgatg gtttcccatt cttccataag agtttcttgt tcttgatcca
    13921 attggtccca ttccttttgc caagcgatta actgcgccgt atcattggag gcatgcatgt
    13981 cttcttgaat agcaccaatg cgctcttcaa tggtttcaag tcttgcttca atctcctcat
    14041 atcggcgggt caagcgacga cgctcttttt gattagcttt ttgtagctga taatcagtgg
    14101 cagaggcttc ttttgtttcc tcaactgtct ctcctgctgc taggcgggct agttcttcta
    14161 gctctgcctt tttctcaagg taataatcat agtcacctaa gtaaagggtt gagccatttt
    14221 cggtaatttc cagaacctta gttgccaaac gattaatgaa atagcggtcg tggctgacaa
    14281 aaagcaaggt accgtcaaag tcaatgaggg cattctctag cacttcttta ctgtcaatgt
    14341 ctaggtggtt agtcggctcg tccagaacaa gaaaattatt gttttccata gataacttcg
    14401 caagaagcag acgggctttt tctccacctg acagcatagc caccgatttt ttgacatcat
    14461 ctccagaaaa gagaaaagcc cctaatcggt ttctgatatc gacttctggc gtagttgaaa
    14521 aatcctgcca taattcttcc aaaactgtgt tgctagacgt caaatgtgat tgggtttggt
    14581 catagtaacc tatttcaaca ttggcaccgt atttcacttc tcctttgatg aaaggaatct
    14641 gtccaataac agatttaatc agagtagatt taccaatacc atttggacca acaatagcaa
    14701 tcgcatctaa tttgtgaacg tctaggttaa taggctcaga taaaacatgt tggttataac
    14761 caatagcagc tttttcaacc gtcaacacca cattgccaga cggcttatct gcctgaaaag
    14821 tcatgtgagc tgattttcta gcactagtcg gtttgtctaa gcgttctatt ttttccaatt
    14881 gcttacgtct cgcttgagca cgttttgtag ttgaagccct gacaatattt ttttgaacaa
    14941 aatcttctaa cttggcaatt tctttttgct gtttgtcaaa ctgcttttct tcggctacta
    15001 atttttcagc ttttaaggtc ataaaacgag aataattgcc aacgtagcga tccaatccat
    15061 gaggagttaa atccaaggtc actgttgcca ccttatccaa aaaataacgg tcgtgactca
    15121 caatgattag agctccttga taattggcca agtagttctc taaccaggca atggtttcaa
    15181 tatctaagtg gtttgttggc tcatcaagga ccaatagttc tggcttttct aaaagcattt
    15241 tagccaaggc cagacgagtg ttttgacccc ctgacagctc agcgatagtc atctgccaca
    15301 tgctttcatc aaatttaaag ccatttaaaa tggctttaat ctcagcttca tatgtaaagc
    15361 caccttgctg acgaaaacgt tctgctaaaa gatcataatc catcatcagc cgtgtcaagt
    15421 cctgacctga aacggttgcc atatccattt ccatctgacg gagacgcttt tcatcttggc
    15481 gtaatgcttc gaaaaccttg agcatttcct catatatcgt tttgtcagat tcaaaacggc
    15541 tattttgagc cagataagac aaggttaggt cttttttagt attaacttct ccgctcgttg
    15601 gggtttcttc cccaaccaat agcttgagta aggttgactt accagctcca tttggcccca
    15661 ctaatgcaat tctgtctctt tcatcaactt gcagtgatat gttttgaaaa agaacatctc
    15721 ctgagaaaga gcgttctaac ttgtttcctt gtaaaataat catactttca ttctaacaaa
    15781 aaaatgccct tttgaggaca ttttcagcct agtttccaaa agaaatcgaa ctctttttaa
    15841 gtcgttgatt accttcttta gcgctaacaa ggaaagtgga atgactatta ccactcccat
    15901 gacgcgtctt tgcagatggc ttttgtgtta agatatcaaa aatttgcacc acttgctcag
    15961 taactatcga gatcttattg gctaaaacgg aatcactgtg attagaatag tacatcagcc
    16021 ctataataat aatcgttact aataagatag tttgtaaaaa ccctttgata agatgccaaa
    16081 acattttcac tattagctta ggcagccaca atatgatcgc cagtaattgt ttcatttgtc
    16141 acctcatgtt gtactaggaa atggttaaga caacctttct tctttttggt agtttatcgg
    16201 taaccactct aacaggactt ctaattgctg gttccaatag taccactcat gtttaccatg
    16261 acctttatga taatcaatgt caagaccttg agcctgaaaa tcagcaatag ctttttcatt
    16321 agtggcaaaa agaaaatcct catagccaca ccaagcataa aatttggttt ttccatcact
    16381 ttctgccacc atatttttaa ggtaatgctt gtcaaggtca gggtcatcaa actggccaaa
    16441 aacgccctgc caataggcta attctcccag gtttccctca agcaaggtct caggagaaaa
    16501 atccaaagcg cctgaaaacg atgccgcata agaaaaccga ttgcttttga gtgcccactt
    16561 aaaggcacca tagccaccca tagagagccc agcaacaaag gttttttctc gtttctgggt
    16621 catattagga aagaaagctg ctaagacctg cggtaactct tgtgacaacg cccgataata
    16681 gttaagtcca taagccgtgt ctgtatacca acctaggtct gtcgaaggca taacgactat
    16741 caaattggta tgccgcaaca agcgttcaat agctgttcgt ttttgccaag agttttcatt
    16801 gcctcccata ccatgtaaca aatataaaac aggaatatcc ttatcccctt gatctttttt
    16861 agggatttca gactggtcag gataaatcac atttacctta cgctccattc ctaaaactac
    16921 cgaatggtat tcaattgcaa tacttgccat tcattacact cctttatacc aaaataatcc
    16981 aatcacgtaa atctactgta tttttgggtg aacagtttcc tggtttacca gttgttaaaa
    17041 tcaaagaagg taggcagttt ttgcctaacc ttcctatttt cactaacgaa cttccatcac
    17101 aaccggcaga atcgctgggc gccttttggt ttgttcaaat aagaatttag acaaatcatc
    17161 tctgacattc ccttttaatt cgccccagtc aaagg
//
LOCUS       DAUVUY010000034        16193 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.034, whole genome shotgun sequence.
ACCESSION   DAUVUY010000034 DAUVUY010000000
VERSION     DAUVUY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16193
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.034"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            517..1044
                     /locus_tag="VOX58_000980"
     CDS             517..1044
                     /locus_tag="VOX58_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HES5878213.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1044..2162
                     /gene="yqeH"
                     /locus_tag="VOX58_000981"
     CDS             1044..2162
                     /gene="yqeH"
                     /locus_tag="VOX58_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HES5878214.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPGKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            2188..2496
                     /gene="yhbY"
                     /locus_tag="VOX58_000982"
     CDS             2188..2496
                     /gene="yhbY"
                     /locus_tag="VOX58_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HES5878215.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2565..3197
                     /locus_tag="VOX58_000983"
     CDS             2565..3197
                     /locus_tag="VOX58_000983"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HES5878216.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDFYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            3194..3787
                     /gene="yqeK"
                     /locus_tag="VOX58_000984"
     CDS             3194..3787
                     /gene="yqeK"
                     /locus_tag="VOX58_000984"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HES5878217.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            3811..4164
                     /gene="rsfS"
                     /locus_tag="VOX58_000985"
     CDS             3811..4164
                     /gene="rsfS"
                     /locus_tag="VOX58_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HES5878218.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWYEAPAVALDAYLA"
     gene            4212..4955
                     /locus_tag="VOX58_000986"
     CDS             4212..4955
                     /locus_tag="VOX58_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HES5878219.1"
                     /translation="MKHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEAPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTKTSKRWFFVAYK"
     gene            5209..6315
                     /locus_tag="VOX58_000987"
     CDS             5209..6315
                     /locus_tag="VOX58_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HES5878220.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTEDVID
                     YQKLIKVYEKKSKQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
     gene            6797..7513
                     /locus_tag="VOX58_000988"
     CDS             6797..7513
                     /locus_tag="VOX58_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HES5878221.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            7716..8558
                     /locus_tag="VOX58_000989"
     CDS             7716..8558
                     /locus_tag="VOX58_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HES5878222.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYRVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            8885..9730
                     /locus_tag="VOX58_000990"
     CDS             8885..9730
                     /locus_tag="VOX58_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HES5878223.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            9980..11044
                     /locus_tag="VOX58_000991"
     CDS             9980..11044
                     /locus_tag="VOX58_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HES5878224.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKIQLSADALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            11045..11737
                     /locus_tag="VOX58_000992"
     CDS             11045..11737
                     /locus_tag="VOX58_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878225.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(11791..13161)
                     /gene="brnQ"
                     /locus_tag="VOX58_000993"
     CDS             complement(11791..13161)
                     /gene="brnQ"
                     /locus_tag="VOX58_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HES5878226.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VIASFVHPAGNYGDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEVTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPVVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
     gene            13395..14609
                     /gene="sstT"
                     /locus_tag="VOX58_000994"
     CDS             13395..14609
                     /gene="sstT"
                     /locus_tag="VOX58_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HES5878227.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGKQTNMTLIIVLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVSVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(14664..15338)
                     /locus_tag="VOX58_000995"
     CDS             complement(14664..15338)
                     /locus_tag="VOX58_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HES5878228.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(15348..>16193)
                     /locus_tag="VOX58_000996"
     CDS             complement(15348..>16193)
                     /locus_tag="VOX58_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HES5878229.1"
                     /translation="NLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVDLGVAFKKY
                     FFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIANFSLHQQLM
                     VSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVTTAAITFLL
                     FKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPHIAPIPLLF
                     ESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKEKTIQYATT
                     DILVG"
BASE COUNT         5277 a         2598 c         3326 g         4992 t
ORIGIN      
        1 gtaatcattt tttgatttac agttattatc atgtgttatc tggcgactgt cctgtttaca
       61 agtaagttca caagtatggg ataaaatagt ataaactata gtaaatactg aatagtcata
      121 gaaaaatatc ggcataggtc agaaaagctg atatgtttaa tactttttgc ggatatctac
      181 tgtatttaaa tgtatttgat atccttgtca aggatatacg tctaaacaag ttcaaaacga
      241 aatcatttgt gattatctta tcaggagttg actcttgata agatttttgt atatcagtac
      301 tgtggtacgt ttagatagct tggcagtgaa tttatagagt gtcaaacaac gagtgttttg
      361 tgttacacta accattagaa gagtctattg ttgagcatat caaggtgtta tcttttcgag
      421 ttgaggtatc atatctagta tatttaacta tggttttaaa tgctatttca aaaaaaatat
      481 gataaaatag aaagtactaa ttattttgga gatgacatga gtatcgatga ttataggccg
      541 acctatatgg tagaggctat ttatgattta agagcaaatg atttgttgcg tcatggtatt
      601 acggctgttt tagtggattt agataatacc ttaattgcat ggaataatcc tgatggcaca
      661 ccagaggttc gcgcttggct tgatgaaatg actatcgcgg atattagcgt ggttgttgtt
      721 tcaaataata aacattctcg tgttgagcgt gccgtttcac gctttggtgt tgactttatt
      781 agccgggctt tgaagccatt tgcttatggt attgaaaaag ccattgctcg ttatggattt
      841 gatcgcaacg aggttatcat ggttggagat cagctaatga ctgatatcag agctagtcat
      901 agagctggaa taaaatcagt tttagtcaaa ccacttgttg cttctgatgc ttggaatact
      961 aaaattaacc gctggcgtga acgccgtgtg atggcaaaat tagaagagaa atatggtaaa
     1021 ttatcatatc aaaaaggaat ttaatggaag aattattttg tataggctgt ggtatccaga
     1081 ttcaaactga agacaaagaa aaagctggtt ttacgccagc tgctgctttg aaaaagggta
     1141 tggaaacagg agaactctat tgtcagcgct gctttagatt gaggcattat aatgaaatta
     1201 cagatgtcca tattaccgat gatgaatttt taagacttct tcacgaagtg ggtgatagtg
     1261 atgctcttgt ggttaatgtg attgatattt ttgactttaa tggttctatt attccaggtc
     1321 tatctcgctt tatttctggt aatgatgtct tactggttgg gaataaaaaa gatattttgc
     1381 caaagtctgt taaggatggt aaagttactc aatggttgac ggaacgtgca cacgaagaag
     1441 gtcttcgccc tttagacgtt atgttaacga gcgctcaaaa taaatatgcg attaaagact
     1501 tgattgagcg tatcaatgag ttacgtaatg gtcgagatgt ttatgttgtg ggggtcacta
     1561 atgttggtaa atcgacctta attaatgcca ttattcaaga aattacaggg gataaagatg
     1621 tgattacgac gtcccgcttc cctggaacga cccttgataa aattgaaatc ccactggatg
     1681 acggtagctt catatttgat acgccaggta ttattcatcg tcatcaaatg gctcattatt
     1741 tatcccctaa agagctaaaa atagtgagtc ctaagaagga aatcaagcct aagacgtatc
     1801 aattaaaccc agaacaaact ctctttttag gtggtttgtc tcgctttgac tttatcaatg
     1861 gggagcgtca agggtttaca gctttttttg acaatcaact agaattacat cgtacaaaat
     1921 tagctggagc agatgctttt tatgataagc atgttggaac tttgttaact ccgccaggta
     1981 agaaagagtt gacagcattt cctaaattag ttcgtcatga atttacaatt gatcagaaaa
     2041 tggatattgt tttttctggt ctaggatgga ttcgtgttaa tggtcaaaaa gactcaaaag
     2101 caatagttgc tgcatgggct cctgaaggcg tcgctgtcat tgttcgtaaa gcgattattt
     2161 aaaaataaaa aaagaagttg gaaaaaaatg cttacaagta aacaaagagc tttcttaaaa
     2221 tctgaggctc atagcttaaa accgattgta caaattggca aaaatggatt aaatgaccac
     2281 attaagacta gtattcgtca agctttggat gctagagaat tgataaaggt cactttgttg
     2341 caaaatactg atgaagatat tcatgaagta gcagagattt tagaagaaga aattgggtgt
     2401 gatacggttt tgaaaattgg tcgtatcttg attctctata aggtttctgc taagaaagaa
     2461 aaccgcaagt tgtcccctaa agttaaagca atctagtcct tttatttgta tagggactgg
     2521 ttttatgaga cgaggactat tgtcgcgaga attttggaga aagtatggca ttagaattgt
     2581 tgacgccttt tacgaaggtg gaattagagg aagaaaaaaa agaaagcaac cgcaaacaga
     2641 ttgggatttt aggagggaat tttaatccta ttcacaatgc acaccttgtg gttgcagatc
     2701 aagttcgcca gcaattagga ttagatcagg tgctattgat gcctgaatgt aaaccaccgc
     2761 atgtggatgc caaagagact attgatgaga agcatcgtct tcgcatgttg gaattggcta
     2821 ttgaagatgt tgaagggcta gctattgaaa cctgtgaact tgaacgtcag gggatcagct
     2881 atacctatga tacgatgctt tatttgactg agcagcatcc tgatgttgat ttttatttta
     2941 tcatcggagc tgatatggtt gattatttgc caaagtggca ccgtattgat gaactggtca
     3001 aactcgttca atttgttgga gttcaacgtc caaagtataa agctggaacc tcttacccgg
     3061 tcatttgggt agatcttcct ttgatggata tttcttctag tatgattagg gattttatta
     3121 aaaaaggtcg tcagcccaat tatctcttac ctaagcgggt tttagactac attactcaag
     3181 aaggtttgta tcaatgacat atgaagatta tcttccttat agcaggaccg aactgttagc
     3241 aaaaattgca gagcagatga gccctaaacg gtttaaacat gtacttggcg tggaaaaggc
     3301 agccttgtcc ttggcagagt gttatggttg taacccagac aaagcagggt tagcagctct
     3361 tttacatgat tatgccaagg agtgccctga ccaggtcttt ttagacttaa ttgacaagta
     3421 ccaattatct ccagaattag ctaaatggaa taataatgtc tggcatggta tggttggtat
     3481 ttataagatt caagaagacc tagggctaaa agacaaagat atcttgcgag ctattgaaat
     3541 tcacaccgtt ggtgctgctg agatgacctt gctggataag gtcttatacg ttgctgatta
     3601 tattgaagaa ggtcgcattt ttcctttggt tgatgacgct agaaaaatcg cgaagctcga
     3661 tttgaatcag gctgttgcct atgaaacagt gaacactgtc gcttatttag cctcaaaagc
     3721 gcagcctatt ttcccacaaa ctctggatac ctataatgcg ttttgcagct atttaaagga
     3781 atactaatgg ataatgatat gaggaataaa atgaaaaaag aagaactctt aaaaattgtt
     3841 gtggaagcaa ctgatgaaaa gcgtgctaaa gatattttag ccttggattt agaggggtta
     3901 actagtttga ctgattattt tgtgattgcg agtgcaacaa atagtcgtca attagaggct
     3961 attgcggata atatccggga aaaggtaaaa gaggctggtg gagatgctag ccatgttgaa
     4021 ggtaatagcc aagcaggctg ggtcttgctt gatttgaccg atgtggtggt acatcttttt
     4081 ttagaagatg agcgttacca ctataatctt gaaaaattgt ggtatgaagc gcctgctgtg
     4141 gctctagatg cctatttagc ttaatgttac aaagagacct tgtcaatacg acagggtttt
     4201 tgaggatgat aatgaaacat aattatgaaa aatttgcgtc agtttatgat gctgtgatgg
     4261 atgattctct atatgacttg tggacagatt tttcacttcg acatttacca aagtctaagg
     4321 gtcgaaatcg tttgttagag ttggcctgtg gaaccggtat tcagtcagtt cgttttgctc
     4381 aagcaggttt tgatgtgact ggtttagact taagtcaaga catgctggct attgctaaaa
     4441 agcgtgcgca atctgctaaa aagaaaattg attttattca aggtaacatg ctggatttgt
     4501 cacaggtggg gcagtttgat tttgtaacct gctattcgga ttccatttgc tatatgcaag
     4561 acgaagttga tgttggcgat gtttttaaag aagtttatga tgttttagca aacgatggca
     4621 tctttatttt tgatgtgcat tcaacctatc aaacggatga gtgtttccct ggttattcct
     4681 atcatgagaa tgcagatgat tttgcaatgg tttgggatac ctatgctgac gaggctcctc
     4741 actctgttgt tcacgaactc actttcttta tccaagaaga cgatggacgt ttttctcgct
     4801 ttgatgaagt gcatgaggaa cgaacgtatg agttattgac ttatgatatt ttgttggaac
     4861 aagcagggtt caagtcattt aaggtctatg ctgattttga agataaggag ccaactaaga
     4921 ccagcaagcg ttggtttttt gtagcatata aatagaagaa aaaagtagcc tttttagcta
     4981 taggatttaa tgaattatct tgaatgttac ttatatgaaa agagttcatt aggcattaaa
     5041 tgctattatc aaagcttaga agtattggag acattacgat tgacactatg tctgaaggtt
     5101 gctaccaact ttaaagaaag cattttctat tatacgttgt gcttaagcat ttgatttaac
     5161 tcttattagc cctataaaag actatttagt acaatttgag gagattctat gactgttacc
     5221 ggaattatag ctgagtttaa tccgtttcac aatggacaca aatatttgct agaaacagca
     5281 gagggactaa aaattattgc tatgtcagga aattttatgc aaagaggaga gcctgctctt
     5341 attgataaat ggattcgatc agagatggct cttaaaaatg gtgcggatat tgttgtggaa
     5401 ttgccctttt tcgtttcagt tcaatcagca gactattttg ctcaaggagc tattgatatt
     5461 ctttgtcaat taggaattca gcaattagca tttgggacag aggatgtgat tgattatcaa
     5521 aaactgatta aggtctatga gaaaaaatca aagcaaatga ctgcctattt atcgacgctt
     5581 gaagatacac ttagctatcc acaaaaaaca caaaaaatgt gggaaatctt tgctggagtt
     5641 aaattttcag ggcaaacgcc aaatcatatt ttggggttaa gttacgcaaa ggctagcgct
     5701 ggaaagcata tacaactatg tcctatcaag cgtcaagggg ctgcctacca ttcgaaagac
     5761 aaaaatcatc tgctagcttc tgcaagtgcc attagacagc acctgaatga ttgggatttc
     5821 ataagtcatt cagtaccaaa tgcaggttta cttataaaca atccccatat gtcatgggac
     5881 cattattttt cattcttaaa atatcaaatt ctgaaccatt ctgatttaac tagtattttt
     5941 caggtcaatg atgaattggc aagtcgtatt aaaaaagcga taaaagttag tcaaaatatt
     6001 gaccacctag ttgacactgt tgctaccaaa cgctatacaa aagctcgtgt aagacgtatt
     6061 ttaacctaca ttttggttaa tgctaaggaa cctactttac ctaaaggaat tcatattcta
     6121 gggtttacct caaaaggaca agcacattta aaaaaattaa aaaaaagcag gcctctgatt
     6181 actagaattg gagcagaaac ttgggatgag atgacacaaa aagcagacag catctatcaa
     6241 cttgggcatc aggatatccc agaacaaagt tttggaagaa tacctattat aataaaaaat
     6301 gagaggctca attaaactct aattgagttt ttttgggggc ttgactctaa cattacgttg
     6361 atggtttata cttcagttaa tccaatgagg aaattttata acaataatta aaaaagtcag
     6421 tcaaaagata tgctttcgaa gaacaggcta acgtttttac aagattatat cagtaatcat
     6481 tttgacaatt gtagtaatgg aatgttgcca aaatttagaa tgttgtgtgt tatgaatgac
     6541 aagattttaa aggaatacta attaaacggg aggtcgtgaa ccggaagctg ttgtcactca
     6601 agcgccaaaa tctgtctgta acataatatt gatgtatggg aacgcaatta tttggttttt
     6661 attgtgatgc ttaaaacgat tttatatttc tattttacca aagcttttca aagtgctata
     6721 ataagggagt tagagtcaat attagtaacg ttaatgttga caatattcaa taaaatacta
     6781 ttaaaaggag acaaaaatgg gacgtaaatg ggcaaatatt gttgctaaaa aaactgctaa
     6841 agatggagca acatcaaagg tctatgccaa atttggtgtt gaaatttatg tggctgccaa
     6901 acaaggcgag ccagatccag agttaaatac agctcttaaa tttgtcattg atcgtgcaaa
     6961 gcaagcacaa gtgccaaagc atgttattga taaagcgatt gataaagcta aaggaaacac
     7021 agatgaaact ttcgtagagg gacgctatga aggttttggt ccaaatggtt caatgattat
     7081 tgtggatact ttgacatcaa atgttaaccg tacagcggca aatgtacgta cggcttacgg
     7141 taagaacggt ggcaatatgg gagcttcagg atctgtatcc tacttatttg ataaaaaagg
     7201 ggtcatcgtt tttgctggtg atgatgctga cagtgtcttt gaacaattac ttgaagcgga
     7261 tgtagacgta gatgatgttg aagcagaaga gggaacaata acagtttata ccgccccaac
     7321 agatcttcat aaaggtatcc aagcacttcg cgataatggt gtagaagaat tccaagttac
     7381 tgaacttgaa atgattcctc aatcagaagt agtattggaa ggtgatgacc ttgaaacttt
     7441 tgaaaagctt attgatgcac tcgaatcaga tgatgatgtt caaaaagtgt atcataatgt
     7501 agcagatttt taaattgaca tagaataata aaaagagttg aaaactattt cagctctttt
     7561 taagtgatgc ttttataagt aagtaccaag taacatagtc gttgttagaa gataatgaac
     7621 aatagcaaat cagtatcaac ttgataggaa tcaattatta gtgaactcta attaaaaatg
     7681 gtaaactaga cacaagaaca aaaaggagag ataggatgat taaaaaacaa tatttaggaa
     7741 gggcaacaat tgccctagca tcaacccttg tactagcagc ttgtgggtca tcaaaaacag
     7801 ctgagtcagg aaatcagggg agttctaaag aagtcttatt tgcaacggtt ggaacaacag
     7861 ctcccttttc ttatgaaaaa ggtggtcaac taactggcta cgatatcgag gtggctaaag
     7921 cagtctttaa aggatcggat gattacaggg tgtcctttaa aaaaacagaa tggtcttcta
     7981 tctttacagg tttggactca ggaaaatacc aaatgggtgg taacaatatt tcctttacca
     8041 aagagcgttc agctaagtac cttttttctt atccaatcgg atcaacacct tctgttttag
     8101 ttgttccaaa agacagtgat atcaagagct ttgatgatat ccaagggcat acgacacaag
     8161 ttgttcaagg gactacaagt gttgcacagt tagaagactt taataagaaa cacagtgaca
     8221 acccggttac tctaaaattc accaatgaga acattaccca aatgttaacc aatttgagtg
     8281 aaggcaaggc agatttcaag atttttgacg ctccaacggt taacgccatt attaaaaatc
     8341 aagggttaga taatctcaaa acaattgaac tcacctcaac agagcaacca tttatttatt
     8401 ttatctttag tcaagatcaa gagaaattgc aaagctttgt taacaagcgt attaaagagt
     8461 tgactgctga cggaactctt agcaagcttg ccaaagagca ccttggcggt gattacgtgc
     8521 catcagataa agagttgaaa cttcctacag ctaactaaac catctattaa ccagttattc
     8581 atctagaaga ctggtttttc tgatcggaaa caatattata gtttatgagg ttatgttata
     8641 aaaattgcaa atagccttaa tcaaaatgag atgttaaagt gctttttgag aagtgaatat
     8701 tgtaagaagt atgctttggg ttttagtcct caaataccaa gttttttgtt gactaaacaa
     8761 ataaggatgg ttagcatagc atatagttta aaactatggt atatgctagc cattatcaat
     8821 ttgaaaacta tatctaatta agatacaata atgatcgtaa acaaaatgaa tcgaggataa
     8881 cgctatgtct tttaaaaaat ggattggaat agtcggaatc gctttagcat caactgtatt
     8941 ggttgcttgt tctgaaaaac aagatgataa aaacactcta actattggag tgatgacaaa
     9001 aacagaatct gatcaagcga gatgggataa agttgaagaa ttattgaaaa aagacaatat
     9061 cactttgaag tacaaagaat ttacagatta ctcgcaacct aataaggcag ttgctaatgg
     9121 tgaagtcgat attaatgcct tccaacacta taatttctta aataactgga ataaggaaaa
     9181 taaggaacat ttggtggcca ttgctgatac ctatatcagc ccaattaacc ttttctctgg
     9241 aaccagtcaa gatgggaagg ctaaatacaa atcagtagct gatctgccaa atggtgctca
     9301 aattgcagta ccaaatgatg cgaccaatga aagccgtgcc ctctatgtgc tgcaatcagc
     9361 tggcctcatt aaattgaatg tgtctggtga tcaattagca accattgcca atatctcaga
     9421 gaacaaaaag aaattagata tcaaggaatt agatgctagc caaacggcac gtgctttagt
     9481 atctgctgat gcagctgttg tcaataatag ttacgctgtt cctgcaaaaa ttgactacaa
     9541 aacctctctt ttcaaagaaa aagcagatga caattcaaaa caatggatta acattattgc
     9601 aggtcaaaaa gattgggaaa aatcagagaa ggctgatgct attaaaaaac tcatcaaggc
     9661 ctaccaaact gatgaggtga agaaagtcgt ggaaaaaact tcaaatggta tagatgtatc
     9721 cgtatggtaa attttagcca ttgttataac aagagtgtgg tcgtgtttaa aacacatgct
     9781 ttcttgcatg cttgaggaag agtttacgac taatttacct ttagaaagct atttaagacc
     9841 ttttcccttt taggatttag aaaccttggg aagactattg catgttatct taatagtcat
     9901 gattatgcag gcgatgaaaa cattgctatc gtagattact gtcgccatat cgtactaata
     9961 gaggagttat taaaatctta tgaatgaagc aataattcaa ttagatcata ttgatattac
    10021 cttccgtcaa aaaaaacggg ttatcgaagc tgttaaagat gtcacggttc atatcaatca
    10081 aggagatatt tatggcattg ttgggtattc aggtgccggg aaatcaactc ttgtgcgtgt
    10141 gattaacctg ctacaggcac caacaaacgg gaaaatcact gttgatgggg atgtgacctt
    10201 tgaccagggg aaaattcaat tgtcagctga tgcccttcgc caaaaacgac gtgatattgg
    10261 tatgatcttc caacatttca atttgatggc ccaaaaaaca gccaaagaaa atgtagcatt
    10321 tgcgctccgt cattcgtcgt taagtaagac tgagaaagaa cataaagtta tcgagctgct
    10381 agaacttgta ggtttatcag agcgagcaga taactaccct gcccagttat cgggtgggca
    10441 aaaacaaagg gtggctattg cgcgtgctct agctaacgat ccaaaaattt tgatttcaga
    10501 tgaggcaaca tcagcactag atccaaaaac cactaaacaa attttggcgc tcttgcaaga
    10561 attaaaccgc aaacttggcc ttaccattgt tatgattact catgaaatgc aaattgttaa
    10621 ggatatttgt aatcgtgttg ctgttatgca aaatggtgtt ttgattgaag aaggttctgt
    10681 ccttgatatc ttttcaaatc caaaagaagc cttgacacag gaatttatca caacagctac
    10741 agggattgat gaggctttag aaaaaattaa ccaacaagac attgttaaac atttgcctgc
    10801 aaatgccctt ctagctcagt tgaaatatgc aggaacttct acggatgagc ctctacttaa
    10861 tagcatttac cgtcaatttg aagtgacagc taatattttg tatggcaata ttgagatttt
    10921 agatcatatt ccagttggtg acatgattgt tgtcttggaa gggcaagctg aaaatattct
    10981 tgcggctgag aaagccttgc atgaagctgg tgttgatgtt agtattttaa agagaggagc
    11041 ctagatgtca cagttaattc aaacttattt gccaaatgtt tatgaactcg gatggtctgg
    11101 agatgctggt tggggcttag cgatttggaa tactttatac atgaccattg tgccttttat
    11161 tgttgggggt gctattggtc tcttattagg cttattatta gttttaacag gaccggacgg
    11221 tgttattgaa aacaaaacga tttgttgggt tattgataaa gttacctcta tttttagagc
    11281 cattcctttt gttattttga ttgctatttt agctagcttt acctatttgc ttttaagaac
    11341 aactttagga gcaacagcgg ccttggtacc tttaaccttt gcgacttttc ctttttatgc
    11401 ccgtcaggtc caagttgttt tttcagaatt agacaagggt gttattgaag cagcgcaagc
    11461 ttcaggtgct actttctggg atattgtcaa agtgtatctc agtgaaggtt taccagatct
    11521 tattcgtgtt tctacagtca ccttaatttc tttagttggg gaaactgcca tggcaggagc
    11581 tatcggtgct ggaggattag gaaatgtagc gatttcttat ggttataatc gttttaacaa
    11641 tgatgtgact tgggtagcga ctattattat tcttttaatt atttttgcta tccaatttat
    11701 tggtgatagc ttgactagaa ggtttagtca taaataaacg aaacaacacc ctaagttttt
    11761 acttagggtg ttgttgttta gatgatgtca ttagcttttc ttcgttttac ttagtataag
    11821 ccctattaaa aaaccaatag cagcaaagat aatccatccc attgagaatt gccctagtgg
    11881 aacagttttt tggaaaaaag ttacaacggc ttctggtaaa tgaaataatc cagtcatttg
    11941 tgatagtgtt aggagtgcat caaatatggc aggaataaag gttagaccaa ttgtagttcg
    12001 atagacaaca ggatcattat tgaagcattt ttgtgccaaa acaaggaaaa ttaaatcgac
    12061 tgttaatggg tataaaagga ataaaacagg agctgaccag ttgataataa ctgataaacc
    12121 accaaaataa aagaaagctg atagtaaagt aaagatagtt gcccaagcaa tatgagataa
    12181 agcaggcact aatttatgga agtattcagc acttgacgtg attaagcctg ttgaagtggt
    12241 tagacaggcc aggaaaatca caacagctag aaatgcttgt ccgatgccac ctaggtaaaa
    12301 acgagaagca tgacttaaga tttgaccgcc attaactgga ttaccatgaa gggtaaagct
    12361 gccatcacta aaaggaaata atgattgtga tgttgctcca atacgaccga caaagatata
    12421 gacaagtgct aatagcaaaa tggcaatagc cccagaaata agtgttattt tggtcacttc
    12481 tttgtccgtc ttagcgccaa attgtttggt agcctcaatg actaaaatag caaaaacaag
    12541 agaagctagt gcatccatag tgccataacc ttgaattaat cctgctataa aaggaaaatc
    12601 tttaaaggca ttattaacac cgaccccagc gttaaaagca tcaccgtaat ttccagcagg
    12661 atggacaaag gacgcgataa ccaaaataga aataacaact aacaacgttg gcgttaaaaa
    12721 ttttccgata ttttcagcta gcttactagg tttgatagca aggaagtagg acagaccaaa
    12781 aaatagtata gcataaatag ctttattggt tgtattatct cctaagatag gagcgatacc
    12841 gactgaaaaa gaagtggctc ctgttcttgg aatagcaaaa aatggaccaa tagataaata
    12901 caaaatggaa gaatagaaga ttgcatacca tttagagatg gggcgtgcca aactttcaac
    12961 atcgcctgat ccagatttag caacagcaat gacaccgagt aaaggcaagg agacaccagt
    13021 taaacaaaaa ccaatgatag accatagaat ctgatgacct gagtagatgc ctaaaaaagc
    13081 tgggtaaatg aggtttgctg ctccaaaaaa tagtgcaaat agcatgaatc caatgacgat
    13141 atatacattt ttttgtttca tttataaata gtctttctat tgatactaat ccgaagattc
    13201 taacaatttt aattatatta tgtgtcaaaa taacaacaaa ggttattata tctaagatta
    13261 cttcgatagt caatagaaat tgactgaaaa ttcttagcac aaaaaatggt taagatatgt
    13321 agctcattcg atttgacgaa tagatagaat tatattatta taaaaaacat gtaactttag
    13381 taaggatgta acgtatgaaa aaaatatatg atttatgggt tagggttagt ctaatcaaaa
    13441 aaataggtat cggtgttgta ataggagtca tgttgggcat tttggctcct gatcttacag
    13501 gttttagtat tttaggaaag ttatttgttg gtggtttaaa ggctatcgca cctttgcttg
    13561 tatttgcttt ggtttcccaa gccatctccc atcagaaaaa agggaaacag actaatatga
    13621 cattaatcat tgttctgtat ttatttggta cctttgcgtc agctttagta gcagtactga
    13681 cagcttatct attccctttg acgttagtgt taaatacacc tgtaaataca gaattatcac
    13741 cacctcaagg tgttgctgaa gtttttcagt cacttctatt aaaattagtg gataatccga
    13801 tcaatgcttt agctactgcc aattatattg gtgtgctatc atgggcaatt atttttggac
    13861 tagcattaaa ggctgcaagt caagaaacaa agcatcttat aaaaacagcg gcagaagtta
    13921 cttcacaaat agttgtttgg atcatcaatc tggcacctat tgggattatg agtctggtgt
    13981 tcactacgat ttctgaaaac ggtgttggaa tcttatctga ttatgctttt ttgatactag
    14041 ttttagtagg aacaatggtt tttgtcgcct tagtggttaa tccacttatt gcggttttga
    14101 ttacacgaca aaatccttat ccccttgtct tgagatgttt acgtgagtca ggtcttacag
    14161 catttttcac acgaagttct gcagccaata ttccagtcaa tatgcaatta tgccaaaaaa
    14221 ttggattgag taaagatact tattcagtgt caattccact tggagcaact attaatatgg
    14281 gtggggcagc aattaccatc aatgtcctaa cgcttgctgc tgtacacact tttggtattc
    14341 ctattgattt tctaacagcc ctcttactta gcgttgttgc tgctgtctct gcttgtggtg
    14401 cttctggtgt tgctggggga tcgttgttac ttattcctgt agcatgtagt ttgtttggca
    14461 tctcaaatga tcttgcgatg caggtggtta gtgttggttt tattgttggt gttatccagg
    14521 attcttgtga gacagcctta aattcgtcaa ccgatgttct ttttacagct attgctgaaa
    14581 atgctttttg gaaacgtaaa aaagcctagc taggtttaac atagtaaaaa acatccttgg
    14641 aaaacgccaa ggatgttttt ttattattta agatagccaa gataatcaaa tttttcaaag
    14701 gtgtggtcat tagcgatggc tacaatgata gtattcggtt ctaaaggttc aaaaggtttg
    14761 acattggtat ctaaggtttt aacttctttt tgacgcatgc caatcacatt taactcatat
    14821 ttacgacgaa catctagttc ggaaagagat tgtccttccc agcttttagg aatgacaaat
    14881 tcaatcatgg aaatgccatg ttcgaggtag atgatgcttt cgatatgacg tcttaacaga
    14941 tttgaagcta cacgttttcc agaatcacgt tcaggggtga tgactttagt tgccccgatg
    15001 ccatatagaa cttcttcaaa aattttattt ttagccttgg caataatggt tggaacaccg
    15061 agttttttac agtgcatgac tgcaagaaca gaagattcca agttattccc tgaagcgata
    15121 acgacagtat cacaatgttc aatccctaca gctaatagaa attctttgtc agtaatatct
    15181 ccaacagcgg ctttagtgac caagtcggcc acctctttaa catgactttc tctgatatca
    15241 atagcaataa cgtcttgatc aaaattactt aattctctgg caacagtacg gccaaaaata
    15301 ccaaggccaa gaacgccgac agttttacgt tttaacatgt tttctcctta acccactaaa
    15361 atatcagtag tggcatattg aatggttttt tcttttcgtt gaatcagact gatgaggaca
    15421 gtaattgggc caacgcggcc gacgaacatc aagacaatga caataagacg tccagcagta
    15481 gacaattgtg gtgtcaaatc catagatacc ccaactgtcg cgatggcaga aatagattca
    15541 aataagagtg gtataggagc gatatgaggc tccacgctaa gtaataaaat aaaacctatc
    15601 ataagaactg caaaaaagaa gataagaact gtcatggtct gcttaattgt cttatttgcg
    15661 ataattcggt tacgaaaggt aacttctgat tgccctgaaa gttccgcttt aaagagtaag
    15721 aaagtaatgg cagcagtcgt cactttaata ccaccagctg ttccaccagg agcaccacca
    15781 ataaccattt gaatcatata aagaatgtta gtcggtgcta gggtatcgtt ataagagatt
    15841 gttgcaaagc cagctgttct catagtgact gtttgaaaaa atgaaaccat aagttgctga
    15901 tgtaaactaa aattggcaat ggttttgctg ttatcttttt ctaaaaacca ggttaagaag
    15961 gttccaagaa aaagaataac agctgtggtt tgtagtacta gacgagactg attggataat
    16021 ttacgaaaag ttgccccata acaatgaggt ctttcaaaaa agtatttttt aaaagctact
    16081 cctaaatcaa cccaaaccgc aaaaccaagt ccaccagaga taatcaagaa cgtaatgata
    16141 acatttaatg taggatttaa cataaagtct tttaaactag aagatcctaa att
//
LOCUS       DAUVUY010000035        15945 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.035, whole genome shotgun sequence.
ACCESSION   DAUVUY010000035 DAUVUY010000000
VERSION     DAUVUY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15945
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.035"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(289..534)
                     /locus_tag="VOX58_000997"
     CDS             complement(289..534)
                     /locus_tag="VOX58_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878230.1"
                     /translation="MLKKINTYRRLEIIKNKLDEASKDSIEDIMLDWFRALHYALKVD
                     DTIDLPTFLEFKDNKALRDKIMADLGDFDPFEDLNIE"
     gene            complement(509..784)
                     /locus_tag="VOX58_000998"
     CDS             complement(509..784)
                     /locus_tag="VOX58_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878231.1"
                     /translation="MNSNLKKRVDKLRRLHSLDNASGIVFVEYADGKYQIKKQDHVPK
                     FIETRLIAEFESEEEAENFLSTIPSGYIIFWDIFPEEVEHVKKNKYL"
     gene            complement(774..1112)
                     /locus_tag="VOX58_000999"
     CDS             complement(774..1112)
                     /locus_tag="VOX58_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HES5878232.1"
                     /translation="MVAMVTSKTITEAAERAGITRGTAHRYLKDITFKKACRRYRSEL
                     MQQTTSLLQSASIEAVEVLRNIMLDDSISPYARQQSAQTILSTAYKAHELDDIVEMVE
                     ELEARFEDEQ"
     gene            complement(1154..1420)
                     /locus_tag="VOX58_001000"
     CDS             complement(1154..1420)
                     /locus_tag="VOX58_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878233.1"
                     /translation="MMSLECLRIYLKKSQFTELEHLLFRIIVLGGYPDDMYFPSRVRT
                     IITSLVNNIRKNLDSEGYRSVEELEEAIEKAISEHDEITQKGGG"
     gene            complement(1417..2205)
                     /locus_tag="VOX58_001001"
     CDS             complement(1417..2205)
                     /locus_tag="VOX58_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878234.1"
                     /translation="MYKQTNKTYADILEKVEAATAQWIIRKKASSLKDDVIITDEDIQ
                     AEKERIFIEKYGTALFDFKIDSYRKELDDLKIKAREQVNQFNDFVKELEQDDRYTERG
                     KVDLYRAERKKAEKTLKEIGEAQHKLNVDIQKIEVESAQNAWKKLESEMTPDSITPSE
                     YQYLEMMLSRNDSDEMRKKLAEKFHYHLMVLEYLNADRKFGEVIIKHPLEGVKNQSVN
                     YIKIGDVRLPEVYESTYIHELLKHYKNDIYFGESYANHEWDEEQ"
     gene            complement(2296..2454)
                     /locus_tag="VOX58_001002"
     CDS             complement(2296..2454)
                     /locus_tag="VOX58_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878235.1"
                     /translation="MQTEVKDGFIYFKENGRIGVVEIPTFGKLTLSSKEGYIHQVDHS
                     FSTKHNQK"
     gene            complement(2468..2713)
                     /locus_tag="VOX58_001003"
     CDS             complement(2468..2713)
                     /locus_tag="VOX58_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878236.1"
                     /translation="MSNQMAVFNQFWAYQTLFQPTAQDIKLAILGYFTGTLWACEVSV
                     LEDVELGYRVIVSYVSCFDKSDRLLKIAQAVENSQLG"
     gene            complement(2709..3035)
                     /locus_tag="VOX58_001004"
                     /pseudo
     CDS             complement(2709..3035)
                     /locus_tag="VOX58_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3037..3210)
                     /locus_tag="VOX58_001005"
     CDS             complement(3037..3210)
                     /locus_tag="VOX58_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878237.1"
                     /translation="MKLKLHTRGGNTITIQGDSAFYDELVKYLLSGRQPNWVTCPSAV
                     INLSDIIAITKEK"
     gene            complement(3216..3389)
                     /locus_tag="VOX58_001006"
     CDS             complement(3216..3389)
                     /locus_tag="VOX58_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HES5878238.1"
                     /translation="MKIKLFYQKHNESLDDFEYRVNQFTLSVSVIDIKLSEATYGNYE
                     DMSTTTSLLVLYR"
     gene            complement(3676..5289)
                     /locus_tag="VOX58_001007"
     CDS             complement(3676..5289)
                     /locus_tag="VOX58_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HES5878239.1"
                     /translation="MSIKENIDKLVSMEWTESIFGQSKMKADYKRQLSKVIELDTLSN
                     IHHNGETYAVSNQEVMYLSESKGRTHKFISGNVKTKSKGKGRDSQKYLVVSFDHQFPP
                     FAKLVVDYLLGRFTFFNNKLYDVNNRQAQVLDDFTIQSLYGFKKDNPFILEILEGIHQ
                     TLNIRPAKQLEAYKISGRDFIIDLKEHRLYRTNPSSDSSFFKFYDVDYHTAIGSKKKV
                     GKFLDYVIADHDSLHNATLQAYYIAQVACGLKSKQNFFISKSGVRTGKGLRHIGLSGI
                     FNKIDVELDLLIKGGFEASNAWGAFAGGEMALATEQGDIVGDKMERVLKIIATEKTHM
                     ARSIGGNLGTVNLTSVLCIDTNRTVALSDEMNGRAVLIQYQNRPEGETDLEREKAFEE
                     YWQTFTEMDKSPKIEGVIGFLLVSLDYFRKNDMKFDWRAVEVVNDDDLDDFQVYLINH
                     LSEKDFVERDSYVRELYNSTYGKNNNQAGQRMRTIGVKSYRKQTTGGRKIPGYKVDNI
                     KRFNKFSGGEKENTNIFDMFEKLNQPLPP"
     gene            complement(5279..5800)
                     /locus_tag="VOX58_001008"
     CDS             complement(5279..5800)
                     /locus_tag="VOX58_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878240.1"
                     /translation="MDYLEVTMTKDCVKIFNFLYKYPTKAYQKDEYFKLLYMHPLDSF
                     LTSFSLSGIRVKVTDKPVLAGWKLVRDIEVRIATGELLEMIEELEICYLRKHQTVSYV
                     EIKFYVVHLLTYGIRSRYDMQFFTKLLFCCGYDQETVIGIYSNITKNTRLSRDFITLQ
                     AKLYQTKKGTHEH"
     gene            complement(5810..6178)
                     /locus_tag="VOX58_001009"
     CDS             complement(5810..6178)
                     /locus_tag="VOX58_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878241.1"
                     /translation="MTYQKIPKYLNHLRTQTMGLIFNHRTIITIRREQMQLLSREAEL
                     ELLEKVGCYLEKRWQLELENKDDWDLIPRQDLLEKLDITGTTLKNWEKEGLKSYQSPF
                     ENSKKVYYRKSDIYNFLSVD"
     gene            complement(6087..6278)
                     /locus_tag="VOX58_001010"
     CDS             complement(6087..6278)
                     /locus_tag="VOX58_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878242.1"
                     /translation="MQELNLTPTQTLILFIVLGLVGLLLSLSKPLIEIDLPEDTQVPK
                     PPQNANYGAYIQSQNHYYN"
     gene            complement(6278..6610)
                     /locus_tag="VOX58_001011"
     CDS             complement(6278..6610)
                     /locus_tag="VOX58_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878243.1"
                     /translation="MKYRVETNPFSKDRYTPEQREMFKNRQLSKDKAEAYFTRLYNQH
                     IAWVIIANVMTEYVNKFRKSATSFEEAWDALGYQQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            complement(6622..6804)
                     /locus_tag="VOX58_001012"
     CDS             complement(6622..6804)
                     /locus_tag="VOX58_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878244.1"
                     /translation="MTSVRTAQLLNNGLGLVGQDLKDVLSALANSVNPSIEKQISHIM
                     IDRANTGEWLANVGKK"
     gene            complement(7025..7591)
                     /locus_tag="VOX58_001013"
     CDS             complement(7025..7591)
                     /locus_tag="VOX58_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HES5878245.1"
                     /translation="MNRQVSIFEEVNNQGGAVQVVTTMTFHDQLLDIYGNIQEPLFRA
                     RDVAEMIDYTKTGQGYYDVQAMLRKVDDDEKVKGFPLEGTTKSFRSGQKVWFLTEHGL
                     YEVLMRSNKPKAKEFRKAVKNILKEIRLNGYYMQGELIQNSQPTPDIDDLSYIKQKLT
                     DLQDVDSLQDLRWKMAKLYHVIETLDEY"
     gene            complement(7607..7807)
                     /locus_tag="VOX58_001014"
     CDS             complement(7607..7807)
                     /locus_tag="VOX58_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HES5878246.1"
                     /translation="MSRLKGYRVMLGLTQKQMAEQLRISLQSYNNKETGKNAFNDRER
                     LAIKTMVAKVKPDITIDELFYS"
     gene            8012..8626
                     /locus_tag="VOX58_001015"
     CDS             8012..8626
                     /locus_tag="VOX58_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5878247.1"
                     /translation="MNRLKELRKEKKLTQEELAGKIGVSKITILRWENGERQIKPDKA
                     KELAKYFNVSVGYLLGYVPNKKIDFQLNLDGTTLHLTKEQFLALENTSKSIKKIKNTV
                     NESVKQEEYIKNASKYYDFEKVGRRLTDRLFEIHTDLIELLMMLDHFPSGELSKSQRE
                     AIFKFYKQLDYFVADTPASFDYFKKILKSYGYKIYTEEDKIDFD"
     gene            9155..10318
                     /locus_tag="VOX58_001016"
     CDS             9155..10318
                     /locus_tag="VOX58_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HES5878248.1"
                     /translation="MNIKEYKKKNGTIVYRTSVYLGVDQVTGKKARTTITASTKKGVK
                     IKARDALNNFAMNGYTVKEKPTVTTYKELTALWWESYKNTIKPNSRQSMEGIVRLHIL
                     PAFGDCKLSKLTTPVIQQQVNKWANNANKGIKGAYANYSFLNNINRRILQYGVTMQVI
                     EHNPARDVIIPRKQNNKEHKVKFFSNQELKQFLNYLDDLDLSSYENFFDYVLYKTLLA
                     TGCRIGEVLALEWSDIDLKKGTIKVSKTLNRYQETNTPKSKAGLRDIEIDRATVLLLK
                     QYKNRQQVLSWDLGRSETIVFTPFTTKYAYACLLRKRLQKHFKAAGVPDISFHGFRHT
                     HATIMLYAGIEAKDLQYRLGHSNISMTLNTYVHATKEGAKKAVSIFETAISNL"
     gene            complement(10475..11086)
                     /gene="rpsD"
                     /locus_tag="VOX58_001017"
     CDS             complement(10475..11086)
                     /gene="rpsD"
                     /locus_tag="VOX58_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HES5878249.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(11816..12088)
                     /locus_tag="VOX58_001018"
     CDS             complement(11816..12088)
                     /locus_tag="VOX58_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HES5878250.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            complement(12105..13472)
                     /gene="dnaB"
                     /locus_tag="VOX58_001019"
     CDS             complement(12105..13472)
                     /gene="dnaB"
                     /locus_tag="VOX58_001019"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HES5878251.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            complement(13502..13954)
                     /gene="rplI"
                     /locus_tag="VOX58_001020"
     CDS             complement(13502..13954)
                     /gene="rplI"
                     /locus_tag="VOX58_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HES5878252.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLTITEA"
     gene            complement(13951..15927)
                     /locus_tag="VOX58_001021"
     CDS             complement(13951..15927)
                     /locus_tag="VOX58_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HES5878253.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYHLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
BASE COUNT         4794 a         3175 c         2626 g         5350 t
ORIGIN      
        1 gcgttcatga aagcccccag tgtggtataa ttgtgttaac agatataatc atgaaggcgt
       61 tccgtgtgga cgtctttttg ttttagtcat tcttgatagc ggaagaacgg ctctaaaatc
      121 gtttctaagc gcttttcaga gcttataata taaaatcatc aacttagcag acagaagcgc
      181 taaaaccatg ttttatttta gtctgatggg gaatagatga cgaaaaaggg agacagtcaa
      241 aaaactatct ccttatgacg accttgtggc gatgctgtca ccatttaact attctatatt
      301 tagatcttca aatgggtcaa aatctccaag gtctgccatt attttatctc tcaatgcctt
      361 gttgtcctta aattcaagga acgttggtaa atcaattgta tcatctactt taagagcata
      421 atgcaaagcc ctaaaccagt ccagcattat atcttcaata ctatctttac tcgcttcatc
      481 gagcttattc ttgatgattt cgagccttct ataagtattt atttttttta acatgttcaa
      541 cctcttcggg gaaaatatcc caaaaaatga tgtaaccact tggaatagtg cttaaaaagt
      601 tttctgcttc ttcttcgctt tcaaattctg ctataagtct agtctcaatg aatttaggga
      661 catgatcttg ttttttaatc tgatacttgc catctgcgta ttctacaaag acaataccag
      721 aagcgttatc taaactatgt agtcgcctta acttatcaac tcgctttttt aagttactgt
      781 tcatcttcaa atctcgcttc tagttcctca accatttcaa caatatcatc tagttcatgc
      841 gctttataag cagtgcttaa gatagtttgg gctgattgtt gccttgcata tggactaata
      901 gaatcatcca acataatgtt acgtagcacc tcaacggctt caatgctggc actctgtaat
      961 aaggatgtgg tttgctgcat taactcacta cgataacggc gacaagcctt tttgaaagta
     1021 atgtctttaa ggtatctatg ggcagttcct cttgtgatac ctgctctttc ggctgcctcg
     1081 gtgatagttt tactggtcac cattgctacc atgaatttct cttgtttagg tgttaaacca
     1141 tgttctataa aaatcacccc cctccttttt gtgtgatttc gtcatgctct gaaatagcct
     1201 tttctatagc ttcttccaat tcttcgacac tacggtaacc ctcagaatct aaattcttac
     1261 gtatattatt aaccaagctt gtgatgatcg tacgaaccct tgaggggaaa tacatgtcat
     1321 caggataccc ccctaacact ataattctga atagcaagtg ttcaagctct gtaaactggc
     1381 ttttcttaag gtatattctt aaacattcca atgacatcat tgctcttcat cccattcatg
     1441 atttgcgtag ctctcgccaa agtaaatgtc attcttgtaa tgcttcaaaa gttcatgaat
     1501 ataagtgctt tcgtaaacct caggcaatcg gacatcccca attttaatat aattaactga
     1561 ctgattttta accccttcta atgggtgctt gatgataact tctccaaatt tacggtcagc
     1621 attcaaatac tccaagacca tcagatggta atggaacttc tcagcaagtt ttttccgcat
     1681 ttcatcacta tcattacgtg ataacatcat ttctaaatac tgatattcac taggggtgat
     1741 tgaatcgggc gtcatttcag attctaactt tttccaagca ttttgagccg attctacttc
     1801 aatcttttga atatcaacat taagtttatg ctgagcttct ccaatctctt taagggtctt
     1861 ttcagctttt tttcgctctg ctcggtataa atccacttta ccacgttctg tgtaacggtc
     1921 gtcttgttct agttccttta caaaatcatt gaattggttt acttgctcac gagctttgat
     1981 ttttaaatca tctaattctt tacggtagct atcaatttta aaatcaaaca gtgctgtccc
     2041 atatttctca atgaaaatac gctctttctc agcttgtata tcctcatcag tgataatcac
     2101 gtcatcttta aggcttgaag ctttttttct aataatccat tgcgctgttg cggcttcaac
     2161 tttttccaaa atatcggcgt aagttttgtt agtttgtttg tacatcgtgt ctttcctttc
     2221 ttaaatgcaa aaagaggtat atccaaaaaa gccattaagc ttcatgaata tacctccgtt
     2281 cttcggtcag tataattatt tttgattgtg tttagtagaa aagctgtggt ctacttgatg
     2341 aatatagcct tccttgctag atagggttag cttaccaaaa gtaggtatct ctactactcc
     2401 tattctacca ttttctttga aataaataaa gccatctttc acttctgtct gcattaaata
     2461 cctcctttca tcctaactga ctattctcaa ctgcttgagc aattttcagc agtctatcgc
     2521 ttttatcaaa acaactaacg taagagacga taacacggta gccaagttca acatcttcca
     2581 acacggacac ctcacaagcc catagcgtgc ctgtgaagta tcctaaaata gctaacttaa
     2641 tatcttgtgc cgttggttga aaaagtgtct gatacgccca aaattgatta aatactgcca
     2701 tctggttgct caacttcctt gattaattca ttttcttgaa ccatgtcatc aagttcttca
     2761 tccgtcatat cctcaggttg gttgtaataa tccttgaagc tgtcacagat acgcttgaag
     2821 accttgctta actttctgtc ctcggcatat tctaatacta actgattagc atgcccgcct
     2881 tgattatcat tgtgataggt ggcatcaatc actggttgct cataagtacc agtcatatag
     2941 cctagaatgg cgtgacaggc tacttgtgcg gtgtcaaagt ctttaaacgt gtagtgaaat
     3001 gtgaatgttt ttggtgtgtc tgaaaatgtt ctcatattat ttctcctttg tgattgctat
     3061 aatgtctgat aaattgatga cggcagaagg acatgttacc cagtttggtt gtcggccaga
     3121 aagaaggtat ttgaccaact catcataaaa ggcactgtct ccttgtatgg tgatggtgtt
     3181 gccgcctcgt gtgtgtaatt ttagtttcat atcagttacc tgtacaagac cagtaaagaa
     3241 gttgtggtac tcatgtcttc ataattgcca taagttgctt ctgaaagttt gatatctatt
     3301 acagatactg atagggtaaa ttgattgacc cgatattcaa aatcatctaa agattcattg
     3361 tgtttttgat aaaatagttt gattttcatg ttttttctcc tgttttggct caaagtggtg
     3421 ggtgtagaca caaccctaaa taaagtggtg gtgtgcttaa aaccgcatgg ttgagccatt
     3481 tgccagcaca accaccacct accgccactt ttctatatag ataagggaga aaaaataaaa
     3541 ataatgtaaa gaaataacat atctactaaa aaaataattc tctctcccct atatagttat
     3601 aggtggttat gggtgattgt actctatccc ttgataccac tgggtttata ccccccacaa
     3661 cccctactgt ctgggtcatg gcggtagtgg ttggtttagc ttttcaaaca tatcaaagat
     3721 attagtattt tctttttctc ctcctgagaa tttgttaaat cgttttatat tatcgacttt
     3781 atagccgggt attttgcgtc ctccagttgt ttgcttgcga taactcttaa cccctatcgt
     3841 ccgcattctt tggccggctt ggttgttatt tttcccgtaa gtagagttat acagctctct
     3901 aacataagag tcacgctcta caaagtcttt ttcagaaaga tggttaatca ggtaaacttg
     3961 aaaatcatct aaatcatcat cgttcaccac ttcgaccgct ctccaatcaa atttcatgtc
     4021 attttttcgg aagtaatcaa gtgatacaag taaaaaacct atcactcctt caattttagg
     4081 gcttttatcc atttcagtga aggtttgcca atattcttca aaggcctttt cacgttctaa
     4141 atcagtttcc ccttctggtc tattctggta ttggatcaaa accgccctac cattcatttc
     4201 atccgacagc gcaacagtgc ggtttgtgtc aatacacaga acacttgtta aattaactgt
     4261 tcctaaattg ccaccgatac ttctagccat atgggtcttc tcagtagcaa taattttaag
     4321 cacacgctcc attttatcgc ctacaatatc cccttgttct gttgctagag ccatttctcc
     4381 accagcaaaa gcaccccaag cattgctagc ttcaaagcca cctttaatca ataagtctaa
     4441 ctcaacatcg attttattaa agataccgct aagcccaata tgtcttaagc ctttaccagt
     4501 tctcacacca gatttagaga taaagaaatt ttgtttcgat ttcagtccac aagcgacctg
     4561 tgcgatataa taggcttgta acgttgcatt gtgcaagcta tcatggtctg cgataacata
     4621 atctaaaaac ttgccaactt ttttcttact accgatcgct gtgtgataat cgacatcata
     4681 aaacttaaag aaagaactat cggaactggg atttgttcgg tataaccgat gttccttaag
     4741 atcaataatg aaatctctcc cagaaatctt ataagcttca agttgtttcg caggtctaat
     4801 attcaaggtt tggtggattc cttccagaat ctctaaaatg aacgggttat cctttttgaa
     4861 gccgtataac gattgaatag tgaaatcatc taacacttgc gcttgacgat tgttaacatc
     4921 atataatttg ttattaaaaa aagtaaatcg tccaagtaga taatcaacca ctaacttagc
     4981 aaaaggtggg aattgatggt caaaggaaac cacgagatac ttttggctat ctcttccctt
     5041 tcctttagat tttgttttaa cgtttcctga aataaactta tgagttctac ctttactctc
     5101 acttaagtac atgacttctt gatttgaaac agcataggtt tccccattat ggtgtatatt
     5161 gcttaaggta tccaactcaa tcaccttaga tagttggcgt ttatagtctg ctttcatttt
     5221 agattgccca aaaattgatt cagtccattc catagatact aatttgtcaa tgttttcttt
     5281 aatgctcatg ggttcctttc tttgtttggt aaagtttagc ctgtaaagtt ataaaatccc
     5341 ttgaaagtct ggtatttttg gttatattgc tataaatacc gataacagtt tcttgatcat
     5401 aaccacaaca aaacaacaac ttggtaaaaa attgcatatc gtaacgtgac ctaataccat
     5461 aagtcaatag atggacgaca tagaacttaa tttcaacgta agaaaccgtc tggtgcttcc
     5521 ttaaatagca tatttctagc tcttcaatca tttccaagag ttcccccgta gcaattctaa
     5581 cctctatatc cctaacaagt ttccaccctg ctaaaacagg tttgtctgta actttcactc
     5641 ggatgcccga tagggagaaa cttgttaaaa agctgtccaa gggatgcata tacagtaatt
     5701 tgaaatactc gtccttttgg tatgctttgg taggatattt atacaaaaaa ttgaatattt
     5761 ttacgcaatc ttttgtcatg gtgacctcta aataatccac aaagtcctcc tagtcaacgc
     5821 tcaaaaaatt ataaatatca cttttacggt aatacacctt cttactgttc tcgaatggcg
     5881 actgatacga ctttaagccc tctttttccc aattttttaa cgtggtacca gtaatgtcta
     5941 gtttttccag tagatcttgc cttggaatta aatcccaatc atctttgttt tctagttcaa
     6001 gttgccaacg tttttcaagg taacagccca ccttctctag tagctctaat tctgcttctc
     6061 gtgataataa ttgcatctgt tccctcctaa ttgtaataat ggttctgtga ttgaatataa
     6121 gccccatagt ttgcgttctg aggtggttta ggtacttggg tatcttctgg taagtcaatc
     6181 tctattaatg gcttagaaag gctaagaaga agcccaacaa gccctaaaac aatgaataaa
     6241 ataagtgtct gtgttggtgt gaggttaagt tcttgcatca tgccgatacc tcacttaaat
     6301 aagtttctag ttcccctgtg tctttctctg aacaaggtaa accgttaacg gctctaaaga
     6361 caatctctgt ggtttgttga tagcctaaag cgtcccatgc ttcttcaaag ctggtggcac
     6421 tttttctgaa tttattgacg tattctgtca taacgttagc aataatcacc caagcaatat
     6481 gttggttata tagtcgagtg aaataggctt cagctttatc tttgctgagt tggcgatttt
     6541 tgaacatttc tcgctgttca ggagtgtatc tatcttttga aaaaggattt gtttctactc
     6601 tatatttcat tatgtcttac ctcatttttt gcctacattt gccaaccatt cgccagtatt
     6661 agctctatct atcatgatgt gagaaatctg tttttcaata cttggattga cgctattggc
     6721 tagagctgat aagacgtctt ttaggtcttg accaaccaat cctaaaccat tgttaagtaa
     6781 ttgtgcggtt ctaactgatg tcattttatc ttccttatta attcttattt tctgtgtagt
     6841 tgcttgtttc ttatactagt actagattca tgctagtttt aaggggtagc accctgatgt
     6901 tgattgtggt ataattaaat aaatacctaa ctaaatccca tacttgcttt ttgtggtttt
     6961 agttaattga agtgaaaagc ctgttcctcg ccaaagttca aggctttttt tgttgtctta
     7021 tttcttaata ttcatctagt gtttcgataa cgtgatataa cttagccatc ttccaccgta
     7081 aatcttgtaa gctgtcaacg tcctgtaagt cagttaactt ctgcttgata tagcttaaat
     7141 catcaatgtc tggggtcggt tgactgtttt ggataagctc cccttgcatg taatagccat
     7201 tcaaacggat ttctttgaga atgttcttga ctgcttttct aaactctttg gcttttggtt
     7261 tgtttgaacg cataagcact tcataaagtc catgctcagt taaaaaccag actttttgac
     7321 cactacgaaa acttttcgtg gtaccctcta aagggaaacc tttaactttt tcgtcatcat
     7381 ctacctttct tagcatagct tgtacgtcat agtaaccttg accagttttt gtataatcaa
     7441 tcatttccgc aacatctcta gctctaaata acggttcttg aatattaccg taaatatcta
     7501 atagctgatc gtgaaaagtc atagtggtta caacttggac agctccccct tgattattga
     7561 cttcttcaaa aatactgacc tgtctattca ttttgttcct gcctttctat gagtaaaata
     7621 attcatcaat cgtgatatct ggcttgactt ttgcaaccat tgtcttaatt gctagtcgtt
     7681 cacggtcgtt aaaagcattc ttgcccgtct ctttgttgtt ataagactgt aaagaaattc
     7741 ttaactgttc cgccatttgt ttttgcgtca gtcctaacat aacccgatag ccttttagcc
     7801 tgctcatggt gtacctcctt taaaaaaatc cctcccgtag gttgaaagtg tgaaaggttg
     7861 cgggagatga aaatatcctt tttggataac ttcaaaaatg attatatatc catttcagtt
     7921 acttgtcaag agttctttta aaatttttat atcacttctt gatacttttt ataatttcgg
     7981 ttataatcaa ctttgaaagg tagtgaaata aatgaatagg ttgaaagaat tacgcaaaga
     8041 aaaaaagcta acccaagaag aattagctgg taagattggt gtatcaaaaa tcactattct
     8101 ccgttgggaa aacggcgaga gacaaataaa gcctgacaaa gcaaaagaat tagcaaaata
     8161 ttttaatgta tcagttggat atttactagg ctatgttcct aataaaaaga ttgattttca
     8221 gctaaattta gatggtacaa ctctccatct aaccaaagaa cagtttttgg ctttagaaaa
     8281 cacctcgaaa agcataaaaa aaataaaaaa taccgtcaat gaatctgtta aacaagaaga
     8341 atatataaaa aacgcttcaa aatattatga ttttgaaaaa gtcggcagaa gactcacgga
     8401 cagacttttt gaaatacaca ctgaccttat tgagctattg atgatgttag atcacttccc
     8461 aagtggtgaa ctttccaaaa gtcaacgaga agctattttc aaattttata aacaattaga
     8521 ttattttgtg gctgataccc ccgctagttt tgattatttt aaaaaaattt tgaagtccta
     8581 cggatataaa atctatactg aggaagataa gatagatttt gattaaacaa ataataaatg
     8641 ctcaggcgag taaatcatgt aaaaacgttt tagcacatgg tcagtttact gaatggatag
     8701 aaaataaaat tggtatccat tacagggagg ctaacaggat gatgtcagtt gcaaaacaaa
     8761 ttctaaatta ggcaacgttg cctaatttgt gaacgtaccc aaatttgggg tttcgtcagc
     8821 gactttaata aatcagctag cttctgtttt tgctcatctg gaagggtggc taataaatgt
     8881 agcgcagttg aacctaaatt tcgaaacgtt tcgaaatttg gcaactcact tatttcaatc
     8941 cactcaccca tgaaaggtga ggctttgtcc aactgttgga caaacctcaa gctaaaattt
     9001 caatcctcac agttgtccaa ctagttttta aagaaccttc tcctgagcta tgaaacgttt
     9061 aattacttct tagcaacata actatcaacc atgataaaac ctaaatacct aactaaatcc
     9121 catacttgct tactgatgtt agaaaggtat tatcatgaac ataaaagaat acaaaaagaa
     9181 gaacggtaca atcgtgtacc gcactagtgt ctatcttggt gttgaccagg taacaggtaa
     9241 gaaggctcgc acgactatta cagcttcaac taaaaaaggg gttaaaataa aagctcgtga
     9301 tgctctaaat aactttgcta tgaatggcta taccgtcaaa gagaagccga cagtaaccac
     9361 ttataaagag ttaacagccc tatggtggga gagttacaag aatacaatca aaccaaactc
     9421 tcgacaatct atggaaggga ttgtaagact tcatattttg cccgcatttg gggattgcaa
     9481 gctatctaag ctaactaccc ctgttattca gcaacaagtt aacaagtggg ctaacaacgc
     9541 caataagggc attaaaggcg catatgctaa ctacagcttt ctaaataata tcaatcgccg
     9601 tatcctacaa tatggcgtta ctatgcaagt tattgaacat aatccagctc gtgatgtcat
     9661 cattcctcga aaacaaaata acaaagaaca taaggtgaag ttcttcagca atcaagaact
     9721 aaagcaattc ttgaactatt tagatgactt agatttatct agctatgaaa acttctttga
     9781 ttacgttctt tataagacct tgcttgctac tggttgtcgg attggagaag ttctagctct
     9841 tgaatggtct gatattgatc taaaaaaagg aactatcaag gtttccaaaa ctttgaatag
     9901 atatcaggag acaaacactc ctaaatcaaa agcaggatta agagacattg agattgatag
     9961 agctaccgtc ttacttctta agcaatataa aaaccgtcag caagttttat cttgggatct
    10021 aggacggtct gagacgattg tttttactcc gtttaccaca aaatacgcct acgcttgttt
    10081 gcttagaaaa aggcttcaaa agcattttaa agctgctggc gtgcctgata ttagtttcca
    10141 cggcttccgc cacacccacg ctacaattat gctttacgct gggatagagg ctaaagactt
    10201 gcaatatagg ttggggcact ctaatatctc aatgacattg aatacttatg tccatgcaac
    10261 aaaagaaggc gctaaaaaag ccgtctcgat ttttgagaca gctattagca atttataatt
    10321 aacaagggtg ccccactttg gggctaccct cttactatac caaaatccga ctaaagagaa
    10381 ctaaaagggt agtaaaatca aaaaaagcac cttggaataa cgccccaaag tgcttagtgt
    10441 caaggctttt aagcctatcc tattcaataa aatattacaa cattttgtta tagaattcaa
    10501 cgacaagtgc ttcattgatt tctgggttaa tttcatcgcg ttctggaagg cgagtcaatg
    10561 aaccttcaag tttttcagca tcaaatgata cgaaagctgg acgtccaaga gtagcttcaa
    10621 cagcttcaag gattgcaggt actttcattg atttttcgcg aactgaaatc acttgacctg
    10681 gatcaacacg gtatgaaggg atatcaacgc gtttaccgtc aacaaggatg tgaccgtggt
    10741 taacgaattg acgtgcttga cgacgagtag ttgctaaacc tagacggtaa acaacgttgt
    10801 caagacgacg ttctaaaaga accataaagt tgaaaccaag agttccttct ttgatttttg
    10861 tagcttgaac gaacaagtta cggaattgtt tttcacctaa accgtatgag aaacgaagtt
    10921 tttgtttctc agctaactgc aaaccatatt ctgagagttt gctacggttg tttggaccgt
    10981 gttgaccagg aacgtagtta cgacgtgcca attctttacc tgtgcctgta agtgataaac
    11041 caaggcgacg tgattgtttc catgatggac cagtataacg tgacatatta catgtcctcc
    11101 tctaaatttt ttgaggaaat aacggtttaa aaaatcctga ttcgtgcagg gggttttcgc
    11161 ctaaacagca aaggttactt gaatagataa ccacctgttg acgagcttca tatttttctg
    11221 ctgttatttc gcacaaagaa cagtttacca taagaaagtt gttttgtaaa gtcagtctat
    11281 ctatcatctg atgatcagtt taatatttta aggtcagata tgggacgaac caccactgat
    11341 gaattaagaa ggctatatta taagaagtta tagttatggt aataaagaca actgtcatta
    11401 acaaaaaata atttaaatgc taatttgcag ttcttaaacc ttgactgttt ttttcttagc
    11461 tctctatcaa aagcacttca ctggaaactc ttaattgctc tgaaatggct agtatattgt
    11521 atttatcagg ggaggtctac tcgttttttc acttcaagat agttttaccg gatacttcaa
    11581 gttcctctat taattacact tgtgtcaaaa ctttatttca cgataatctt ttaaccttag
    11641 tactaagatc atttgcttaa ccatggtgaa gacctctttt ttccttcaat ctaacctaaa
    11701 aatattatct tttataggaa ttttttcaaa ataaaaagaa tacaggcaga ataatcttct
    11761 atcgctaata attatatcaa tttattgtaa agaaaaagcc tctatgaggc aaaaatcaat
    11821 ctaaataacg aattaatgtt ttttctgtca aaatatctga ataggtgtat gattcgacat
    11881 aagaattatc aattgctcct ggagtatcag agctatcact atactcaatg ataaagagcg
    11941 aactgtaaac ctcaataagc ctaccaattt tatttttttc ccgtttccgt ccattctcaa
    12001 gggttaattc tactaactgt ccttcatggg ctcttatgtc ctctttaatt tttttcattt
    12061 ttgcaacatc tgtaaatgca tcgctcattt tttgtctctc tttcttatct ttcttcaaac
    12121 tgggctatac ttgagaattt gttgtattct ttttgaaaca tcagtttgac tgttccacgc
    12181 gccccagctc tatttttctc gaggataact tcaattgtgt tatcttcaac agcttcttca
    12241 gcatcatcac cttctttacg gtaataatcg tcccggtata agaaggctac aatatcggca
    12301 tcctgctcaa tagatcctga ttcacgaata tctgataaaa ctggtcgttt atcttgcctt
    12361 tgctcaacgc cacgagaaag ctgacttagg gcaataactg gtactttcaa ttctttagct
    12421 aggattttaa gctgtcttga aatatctgaa acctcttgct gacgattttc gggtttagtt
    12481 cctgtaatca actgtaagta gtcaattacg ataagaccta aaccaccatc cacttcttga
    12541 gacaatttcc gtgatcttgc gcggatttca gtaattttaa tcccgggcgt atcgtcaata
    12601 taaatcggtg cttctgccaa agctccctga gcaattgtta cattattcca atcctgatct
    12661 gtgagttgcc ctgttcttaa actgtgcgaa tcaaccattc cttctgctgc aagcatacga
    12721 tctactaaac tttcagcacc catttccaaa gaaaaaatag caacagtctt tttttgctta
    12781 gtccccacat tttgcgcaat attaagaaca aaggcagtct tccccactgc tggccgagca
    12841 gctaaaataa ctaattgatc tgggtgtaaa cctgttgtaa tcttgtcaag gtctctaaaa
    12901 ccagttggta aacctgtaac atttgaagtc tgcttagaac gtgcttctaa agcctcgtaa
    12961 ttaactttta gcacatctga aattttgcga aacccactac gattactatg ttcattgagt
    13021 tcaattaaag ctctctcaac tccagcgata acctcttctg gttttaaaat ttcatcataa
    13081 gctaggttga cagattctgt caacctagca ataatatcac gcaacatagc tttctcagct
    13141 acaattttag cataatattc tgcattagca ctagttggga cactattaac tagttcaaca
    13201 atataggata agccaccaat actttgcaga tcatcttgat catctaggat tgttcttata
    13261 gtggttgcat caatggcatc attacgatcg ctgagggtaa tcattgcccg aaagataatt
    13321 ttatgagcgt acttataaaa atcgtctgga ctgataaatt ctctcactgc aatcagctta
    13381 tcaggtgaga taaagattga cccaagaaca gattgctctg ctagtaaatc ttggggttga
    13441 actcgtaatt cagctacttc aggcaacctc ataacctcca aatcttgtga tgttttttat
    13501 attatgcttc tgtgatagtc aatttaatct cagctgttac ttccttatgt aatttcacag
    13561 gaacttcaat tagaccaatg gcgcggatag gatgatctaa aacaatatga cgtttatcaa
    13621 cttttacacc aaattgcttt tgcaattctt ctgaaatctt tttagctgtg atggaaccaa
    13681 atgtacgtcc atctggacca actttttctt gaaattgaac acgtgttttg tcctcatcca
    13741 aaacagcctt gacagcttga gcttctgcta atatttcagc ttgagctttt tcttcagctt
    13801 tttgttttcc ttttaattca ccgatgcttt gactagttgc ttctttagct aaatttttct
    13861 taataaggaa attttgagca tagcctgttg ggacctcttt gatttctcct tttttacctt
    13921 ttccttttac atctgctaaa aaaataactt tcatgattct acttctcctg tttctttcat
    13981 tgtcatatta atagttttca ataataagtg cttcgcttgg ggaagactga tatccgttaa
    14041 ctgacaggca gcaagattaa agtggcctcc tccacctaat ttttccatca cacgttgaac
    14101 attgatttta ctacgacttc tagctgaaat agcaatctta tgagaagctg tttcaactaa
    14161 tacaaagcta gcctcaacgt gagccattga cagtatcgta tctgcagctt tactagcaat
    14221 aacattgcta taaagatgat ttttttctcc tgcagctaca atgatactgt caccaagacg
    14281 ttctccttgt aaaataattt catttatttg cttatattct tcaaaatctg ttgcggatat
    14341 attttgaatt tcaacactat cacttccttt gcttcttagg taactagcga catcaaaggt
    14401 acgacttgtc actcgcgtag aaaaattttt agtatcaagc ataatacctg ccattaaaac
    14461 actggcttgg atcttattta aacgtttttt agcattttga aattgaatta actcagtgac
    14521 gagctccgca gcactacttg ctccactttc aataaaagtt aagatagcat tatcaggaaa
    14581 atcatcatct cttctgtgat gatcaactac aataacattt tgaaactgtt cataaaattc
    14641 tttagaaagt gttaaagata tcttagaatg gtcaaccatt actaaaagtg atcttggagt
    14701 cactaaaccc attgcttgag aaacactgat tagacgagtc tttccgtcag cctgcaatcg
    14761 ctcaatagcc ctttcaatat ctggactcat ctcatctgga ttgtaaacgg caaaactatt
    14821 ttcaataatg ttacctgcga aaaattgcat accaacagct gaacctagtg catccatatc
    14881 aagtttccta tgtccaacaa taaaaacatt gtccaccatc ttgattcgat ctgaaatagc
    14941 tgtcatcata gcacgggtac gtgttcttga acgtttaact gtagaaacag accctccccc
    15001 gaaataaatt ggattcgtat gatccgcatt ttcacgaatg acaatctgat caccaccacg
    15061 aacaagcgca atattaagat tctcaagagc tacctgtcca atctggctat gattttcttc
    15121 accaaaagaa attcctatgc tgagtgttaa agggcgttga gcatcttgag cttctttacg
    15181 aaactcttct aagacagaaa atttattatc catcaaatca tttaatgttt taaagtctgt
    15241 aaagaaatag tagcgatcca tattaacacg acggtaaaaa atccgttttg actccataaa
    15301 ctcatcaatg aagttagcta caaatgaatt aatttttgag gtatctgcat ctgataaatc
    15361 atcagtgata tcatcataat tatcaaccga aataatgcca acaaccggtc ttaacatact
    15421 agcatcagct aattgcctgt ttcccacaaa gctatcaaaa aaataaaaaa ttcctgatga
    15481 tacatcaata taagaagtgt atttattacc agaaacttca aatgtttgag aaatatcttc
    15541 acgacgtttt tccgtaataa tttgctgaat caaactgttt tgtataaaac cctcttcagt
    15601 tgtaaaaatt aattctgcat aaggattata ccattctaca gcgttggtct cttgatcaaa
    15661 ttgaactact ccaacaggca tattatcaag tagcgttttt aggttatctt ctgtttgttc
    15721 atttaagagc tcaatatgag ctaagtctga taagtgataa gcttcttttt gataccataa
    15781 aagagcaaca acaaatagca aaacgagaaa aatagctaat aatattaata tttttgattg
    15841 cataatactg acacaaagtg ccagtagtcc aaataaaatc aatcccatca taatcaaatg
    15901 aatggtttca aaacgaaatt ttttcatctt ataacctctt aagca
//
LOCUS       DAUVUY010000036        15667 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.036, whole genome shotgun sequence.
ACCESSION   DAUVUY010000036 DAUVUY010000000
VERSION     DAUVUY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15667
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.036"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1025
                     /locus_tag="VOX58_001022"
     CDS             <1..1025
                     /locus_tag="VOX58_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HES5878254.1"
                     /translation="VFITAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNK
                     RGNFAIEGIEELREQVDTLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDL
                     ITSPGLINLDFADVKTVMANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQ
                     DVIVNVTGGLDMTLTEAEEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVR
                     QEKAEQVSGFRQPRTFTQTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREI
                     PSAQKVISNHNQNQGSAFGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETP
                     PFFKNR"
     gene            1028..1699
                     /locus_tag="VOX58_001023"
     CDS             1028..1699
                     /locus_tag="VOX58_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HES5878255.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKYMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKESIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            1711..2367
                     /locus_tag="VOX58_001024"
     CDS             1711..2367
                     /locus_tag="VOX58_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HES5878256.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            2372..2626
                     /locus_tag="VOX58_001025"
     CDS             2372..2626
                     /locus_tag="VOX58_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HES5878257.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            2626..3417
                     /locus_tag="VOX58_001026"
     CDS             2626..3417
                     /locus_tag="VOX58_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HES5878258.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVGDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            3427..4185
                     /locus_tag="VOX58_001027"
     CDS             3427..4185
                     /locus_tag="VOX58_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HES5878259.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            4458..7259
                     /locus_tag="VOX58_001028"
     CDS             4458..7259
                     /locus_tag="VOX58_001028"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HES5878260.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRDDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMTENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNTKENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEHESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGIAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESDK"
     gene            complement(7524..7826)
                     /locus_tag="VOX58_001029"
     CDS             complement(7524..7826)
                     /locus_tag="VOX58_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HES5878261.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(7877..8332)
                     /locus_tag="VOX58_001030"
     CDS             complement(7877..8332)
                     /locus_tag="VOX58_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HES5878262.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLSWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(8460..10742)
                     /locus_tag="VOX58_001031"
     CDS             complement(8460..10742)
                     /locus_tag="VOX58_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HES5878263.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            11040..11270
                     /locus_tag="VOX58_001032"
     CDS             11040..11270
                     /locus_tag="VOX58_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HES5878264.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            11397..12083
                     /locus_tag="VOX58_001033"
     CDS             11397..12083
                     /locus_tag="VOX58_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HES5878265.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSAFLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMAQGGNH"
     gene            12083..12817
                     /locus_tag="VOX58_001034"
     CDS             12083..12817
                     /locus_tag="VOX58_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HES5878266.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            12966..14375
                     /locus_tag="VOX58_001035"
     CDS             12966..14375
                     /locus_tag="VOX58_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HES5878267.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCESIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            14553..>15667
                     /locus_tag="VOX58_001036"
     CDS             14553..>15667
                     /locus_tag="VOX58_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HES5878268.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYEEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIET"
BASE COUNT         4790 a         2707 c         3282 g         4888 t
ORIGIN      
        1 tggtatttat tactgccggt atgggtggtg gctctgggac aggggctgca ccggttattg
       61 ctcgtatcgc taagagtttg ggagccttga cagtagctgt tgttactcgc ccgtttggtt
      121 ttgaaggtaa caaacgtggt aattttgcta ttgaaggtat cgaagaactc cgtgaacaag
      181 ttgatacttt gttaattatt tcaaataata accttcttga gattgttgat aaaaagacac
      241 ctttattaga agcacttagt gaagctgata atgttttacg tcagggagtt caagggataa
      301 ccgacttaat tactagtcct ggccttatca atctcgattt tgccgacgtg aaaacagtta
      361 tggcaaataa agggaatgcc ttaatgggga ttgggattgg ttctggagaa gagcgcattg
      421 ttgaggcggc gcgtaaggca atctattcac ccctattaga aacgactatt gatggtgcac
      481 aagacgttat tgtgaacgtt acaggaggtc tcgacatgac actcacagaa gctgaagaag
      541 cctctgaaat tgttgggcaa gctgctggtc aaggcgttaa catttggtta ggaacatcta
      601 ttgatgatac tatgaaagat gacatccgtg tgactgttgt agcaactgga gtgcgccaag
      661 aaaaagccga acaagtttca ggttttcgtc agcctaggac ttttacccaa accaacgcgc
      721 agcaagtagc gggtgcacaa tatgcatcag atcaagcaaa acagtcggtt caaccagggt
      781 ttgatcgtcg ctcaaatttt gattttgaca tgggggagtc tcgcgagata ccaagtgcac
      841 aaaaggtaat ttctaatcat aatcaaaatc aaggttctgc ttttggaaat tgggatttga
      901 gacgtgataa tatttctcgt ccaacagaag gtgaattgga taaccatctt aatatgtcaa
      961 cgttctcagc taacgatgac agtgatgatg aattagaaac gcctccattc tttaaaaacc
     1021 gttaataatg gatttactga caaataaaaa gaaaattttt gagactatcc gcttatctac
     1081 agaggcagca aataggacta atgatagtgt ttcagttatt gctgtaacaa aatatgtgga
     1141 tagtacaatt gcaggtcagc ttatcgaagc aggaattgag cacattgccg aaaaccgtgt
     1201 tgataaattt cttgaaaagt atgatgcgtt aaagtatatg ccagtaaagt ggcatttaat
     1261 cggtacctta caacgtcgta aagtcaagga agttatcaat tatgttgatt attttcacgc
     1321 tctagattct gtgagattag ctttggaaat caacaagaga gctgaccatc ctgtgaagtg
     1381 ttttctacaa gttaatattt ctaaagaaga gagtaaacat ggttttaaca tttctgagat
     1441 tgatgaagcg attgaagaaa taggtaagat ggagaagata cagttagttg gtttaatgac
     1501 tatggcacca gcaaatgcca gtaaagaaag tattataact atttttcgac aagcaaatca
     1561 attaagaaaa aacttgcagt taaaaaaaag aaagaatatg ccttttacag aattgagcat
     1621 gggcatgagt aacgattatc caattgctat tcaagaaggc tcaactttta ttcggattgg
     1681 tagagctttc tttcactaat ggagaataag atggctttta aagatacatt taacaagatg
     1741 atttcttatt ttgacacgga tgaggttaac gaagttgaag aagatgttgc agcatcaact
     1801 gataacgtta ttccaagatc acaacaatca gtcagagcaa gtagtcatcc aaaacaagaa
     1861 cctagaaaca atcacgtaca acaagatcat caggcgagat cccaagaaca gacaaggtca
     1921 caaatgcatc caaaacatgg tacttctgaa cgctattatc agcagtctca gccaaaagaa
     1981 ggccatgaaa tggttgacag aagaaaacgg atgagcactt ctggtattgc aaatcgccgt
     2041 gagcagtatc aacaatcaac ttgttcagat cagacaacta ttgccttaaa atatcctcgt
     2101 aaatatgagg atgctcaaga aattgtggat cttttaatag ttaatgaatg cgttttgatt
     2161 gattttcagt ttatgctaga tgctcaggct agacggtgtt tagattttat tgatggtgct
     2221 agtaaagtgc tctatggtag cttacaaaag gtcggctctt caatgtactt actggctccg
     2281 tcaaatgtat ccgtcaatat agaagaaatg actatcccac atactacaca agatattggc
     2341 tttgattttg atatgaaaag gcggtaaata aatgatatta atactatcta ttcttcttcg
     2401 tctgatcaaa gtttacactt atttattgat tgcttacgca ttaatgtcat ggtttcctgg
     2461 ggcatatgat tcaaaaattg ggcgtttgat tagtggtatc gttgaaccaa ttttaaaacc
     2521 ttttagagca tttaatttac aatttgccgg tcttgacttc actatttttg tcgtcattat
     2581 tagtttgaat tttttagctc aagttttggt ccgtgtgttt atttaatggt tagtcatagt
     2641 aagatttatc agcattttca ccaagaagaa tatcctttta ttgatagaat gtctgatatg
     2701 attaatagag ttggagatta ctatctttta gaagttactg agtttttaaa tcctagagaa
     2761 gtgatgattt taaaaagttt gattgcttta acagatctaa aaatgttcgt atcaacagat
     2821 tactacccaa gcgaatatgg tcgtgtcatt attgcacctg gttactatga cttagaacaa
     2881 agtgattttc aaatagcttt agtagagata agttatcagg caaagtttaa tcagttgaca
     2941 catagtcaaa ttttaggaac tttaattaat gaattaggag taaagcgaaa tttatttgga
     3001 gatgtttttg ttgaaatggg atatgcccag ctcatgatta agcgggagtt attggattat
     3061 tttttaggaa caattactaa aatagctaaa actagtgtga aattaagaga agttaacttt
     3121 gatcagttaa ttaggtctat tgataacagc cagaccctgg atattctagt ttctagtttt
     3181 cgattagatg gtgtagttgc tactatctta aaaaaatctc gaacgcaagt tatagcatta
     3241 attgaagcaa ataagattaa ggtaaactat cgactcgcta ataaagcttc agataatcta
     3301 gtcatagggg atatggtgag tatcagaggt cacgggcgtt ttactctttt agcagataat
     3361 ggagtgacca aacatggcaa acaaaaaata acactaagta aaatgataca taaataagga
     3421 gatacaatgg cacttacaac gctagaaatt aaagataaaa cttttaagac aaaattccga
     3481 ggatactgtg aagaagaagt caatgaattc cttgatattg ttgttgatga ttacgaagct
     3541 cttgtacgta aaaatcgtga taacgaagca agaattaaag atcttgaaga aaaattatct
     3601 tactttgatg aaatgaaaga gtcacttagc cagtctgtaa tcttagctca agaaacagct
     3661 gaaaaagtga aagcaacggc taatgcggaa gcaacaaact tggttagtaa agcgacttat
     3721 gatgctcagc atttattaga tgaatctaaa gctaaagcca atcaaatgtt gcgtgatgca
     3781 actgatgagg ctaagcgagt tgcgattgaa acagaagaac taaagcgtca aacacgtgtg
     3841 ttccaccaac gtttaatctc atctattgag tcacaattaa gcttatcaaa ttcacctgaa
     3901 tgggatgaac tgctacaacc gactgcaatc tatcttcaaa attctgacga tgcttttaag
     3961 gaagtcgtga agaccgttct taatgaagac attcctgaat ctgatgatag tgcctctttt
     4021 gatgcaacac gtcagtttac tccagaagag ttagaagaat tgcaacgtcg tgttgatgaa
     4081 agcaataagg agttagaggc ttaccaactt gactctcaat ctgattctac gactgagcca
     4141 gaggtaaatc tcagtgaaac acaaacgttt aaattaaata tctaaataaa gaaaaacaag
     4201 atgatttgct tataatcaca gcaagcggga gatgatgaga gtctcgtatt ccctaagtat
     4261 tatcattatc attttctaaa attttcgctg aactacagta agcgaggagg tttgttcacc
     4321 ttacgagcaa agagggagcg gtaagtctta tgatatatta tcgctctgaa ctaaggtggt
     4381 accacgagct ttcgtccttt ttgagacgag gcttttttcg ttatagtcgc aagtttgttt
     4441 aaaaggagtt tgaaataatg aaattaaaag agacacttaa tctaggaaaa acagcctttc
     4501 caatgcgcgc aggtcttcct aataaagagc cacaatggca agcagcttgg gaacaagcag
     4561 agctttacaa aaaacgtcaa gaattgaatg caggtaagcc tgcctttcat cttcatgatg
     4621 gacctccata cgctaacggc aatattcacg ttgggcatgc ccttaataaa atttcaaaag
     4681 atatcattgt gcgttctaag tccatgtctg gctttcaagc gccttatgta cctggttggg
     4741 acacacacgg ccttccaatc gaacaagtct tggctaagca aggaatcaaa cgtaaggaga
     4801 tggatttagc agaatacctt gagatgtgtc gtcaatacgc tttaagtcag gttgataaac
     4861 agcgagatga tttcaaacgg ttaggtgttt cggcagactg ggaaaatcct tatgtcactt
     4921 tggacccaca gtttgaagct gatcaaattc gtgtttttgg agcgatggct gaaaaaggtt
     4981 acatctatcg tggtgctaaa cctgtttatt ggtcatggtc ttcagaatcg gccttggcag
     5041 aagctgaaat tgaataccat gacatcgatt caacctcttt atactacgcc aataaagtta
     5101 aagatggtaa aggtatcctt gatactaata cgtatatcgt tgtttggaca acaacaccat
     5161 ttacggttac agcatcacgc ggtttgactg ttggtcctga tatggattat cttgtggtga
     5221 aacctgctgg ttcagaccgt caatatgttg tggcagaagg tcttttggat agccttgctg
     5281 gaaaatttgg ctgggaatct tttgaaactt tagctagcca taaaggagct gatttagagt
     5341 acattgtgac agaacaccca tgggatactg acgtagaaga attggttatc cttggtgacc
     5401 atgttaccct tgagtcaggg acagggattg tccacacagc accaggtttt ggtgaggatg
     5461 actacaatgt tgggacaaaa tataaactgg aagttgctgt gactgttgat gaacgtggcc
     5521 taatgacgga aaatgcaggt ccagatttcc acggacaatt ttataacaag gtaacgccga
     5581 ttgtcattga taaacttggt gatcttttat tggcacaaga agtgatcaat cactcttacc
     5641 catttgactg gcggacgaaa aaaccaatca tctggcgtgc tgtaccgcaa tggtttgctt
     5701 ctgtttctga tttccgtcaa gatattttgg atgaaattga aaaaacaacc tttcatccat
     5761 catggggtga aacacgcctt tacaatatga ttcgtgatcg tggcgactgg gttatttctc
     5821 gtcaacgtgc ttggggagtt ccgcttccta tcttctatgc cgaagatggc acagctatta
     5881 tgactaaaga agtgacagat catgtggctg atttgttcca agaaaatgga tcaatcattt
     5941 ggtggcaaaa agaagctaaa gacctattac cagagggctt cactcatcca gggtcaccaa
     6001 atggcgagtt tacaaaagaa actgatatca tggacgtttg gtttgactct ggttcttctt
     6061 ggaatggagt catgaatact aaagaaaatc tttcctatcc agcggatctc tacttagaag
     6121 gctctgacca gtatcgtggt tggttcaatt catccttaat tacatctgtg gctgttaatg
     6181 ggcatgctcc atataaggct attttatcgc aaggctttgt tcttgatggt aagggcgaaa
     6241 aaatgtctaa atcaaaaggc aatatcattt ctcctaatga tgtggctaaa caatatggtg
     6301 cagatattct tcgactttgg gtagcttctg tggataccga taatgatgtc cgtgtttcta
     6361 tggagatcct tggtcaagtc tctgaaactt accgtaaaat ccgtaacacc cttcgcttct
     6421 tgattgctaa tacatctgat ttcaaccctg ctacggatac agtagcttat gcagatttag
     6481 gagctgttga taagtacatg acaattgtct ttaatcagtt ggtagcaacg attactgacg
     6541 cttatgagcg ttatgacttt atggctattt acaaagcagt ggtgaacttt gttacggttg
     6601 atttatcagc cttctatctt gattttgcta aagacgttgt ctatattgaa gctgccaata
     6661 gcttagaacg tcgtcgcatg cagacagtct tctatgatat cttggttaag attaccaagt
     6721 tattgacgcc aatcttgcct cacactactg aagaaatctg gtcttatcta gagcatgagt
     6781 cagaagcatt tgttcaattg gcagaaatgc ctgtggcaga aactttctca gctcaagagg
     6841 atattttaga agcttggtct gctttcatga ctttgcgtac ccaagctcaa aaagctttgg
     6901 aagaagcgcg aaatgctaag attatcggta aatcattgga agcccatttg accatctacg
     6961 ctagcgaaga agtgaaaacc ttattgactg ctttagacag cgatattgct ttgcttttga
     7021 tcgtgtctca attaaccatt gctgacttgg cagatgcgcc tgcggatgca gtggcatttg
     7081 aaggtattgc ctttatggta gaacatgcca taggtgaagt ttgtgagcgt tcacgccgca
     7141 tcgacccaac tactcgcatg cgttcttaca atgcatttgt ctgtgatcac agcgctaaaa
     7201 tcattgaaga aaatttccca gaagctgtgg ctgaagggtt tgaagagagt gacaaataag
     7261 tcttatcaag acttgaagtg acactgagta agaagacaac catcgggtct tagggcctga
     7321 tggtttttta gtagagctga ttacgtccat tagccagctt taacgagaga caaagtagga
     7381 gaaatttaga gtactttttt aatttctttt aagaaaattg ctggcttcat ataaggacta
     7441 ttaagataga gttcaccttt cgctaataaa aaaacttagc tcttttagaa gggctaagtt
     7501 ttgtctgaat agctggctgc tgtttataca gaagtttgaa atgaaatttc aataaggtta
     7561 ttcgtgtgaa gtgtttttga ttgtccagga ataacaattt taggatcaat tttacgtttt
     7621 aaaaaaaatt cgcgaatgat atcaagttca tcctccttga tggcacggta tttatttgaa
     7681 ataagtagtt cgtaatgttt tggtgcttgg gtaaaaatga catctgtatt tcctgctgag
     7741 tatacttcga ctaaataggc atcggtgttt tttagctgat ttctgatgag ttctgggtga
     7801 ctgctagtgg tattgatgag tctcataaat gtcctcctta gtattcctgt acctattata
     7861 gcactaattt aaagccttag ttagtagaat gatgcttttt ttgccattcc ttgactcccc
     7921 attgatggct ctctcgtttt aatttagcaa ttgcctcgat aggagaaaac caacttaagt
     7981 tattaaaatc ttctaagggc ttagaaacgg cttgaaatgc ggttacttca taaagatagg
     8041 caggatggta gaagtgggta tcacgatggc gagagtagaa atattcatca gcctgaccgt
     8101 aatatgaacc aatagtagca gaaaaaccaa gttcttcaat cagttcacgc tctagggcct
     8161 gaagctgacc ttcacctgct tcaatttcac caccaggtaa aaaccaggaa ccattaggag
     8221 cttgaacaag aataattttt gtttgttcgt gattgggaat gatagcatag acaccgtaac
     8281 gtgtgacata gtctttatgt gcatttttat gtccaaatgt tggaatcatc attttattta
     8341 cctttccttt ttcttcatta tactaaattt ttgctaatta ttcttatcaa tttgtttagt
     8401 ttaataaaaa atcgatagaa aaagagagag tctccttata gaaactctct tagctaacgt
     8461 taatctagag actcttcgtt aagaaaagaa tctccctctt taacagcttt aatcgtgatg
     8521 ttttcactgt ttgtcatcag tgcacgtaac tgtttttcag ttgggtgttc taaataatag
     8581 tctgtaatgg catcttcgat ataatcttgg atagtacgac ggagcggccg agcgcccatt
     8641 ttaggatcat agcctaaatc tactaatttt tctttgacct tttgtgtcac atcaagatga
     8701 atcccattat agcctaagcg ctcattaaca tcttccagca ttaaatcaac gatatgcagc
     8761 aaatgctctt tagataaagc tttgaattca ataataccat cgaaacgatt cataaactct
     8821 gggctgaaga agttgcttaa ttcaccaagg acagaacttg tccgtccttc tctagcagca
     8881 ccaaatccga cagaagcttc gcttttacct gttccggcat tagaggtcat gataataatg
     8941 gtgtccttga agctgactgt tcgcccttgg ccgtctgtta agcgaccgtc atcaagaact
     9001 tgtaagaaca tgtgcatgac gtcaggatgg gctttttcca cctcatctaa gagaataagg
     9061 gaataaggat tgcgacgaac ttgttcggtt agctgtccag cttcttcata gccgacataa
     9121 cctggaggag ccccgactaa tttggcgaca gcgtgttttt ccatgtattc ggacatgtca
     9181 aagcgaatca tattgtttgt cgaaccaaag agttcaatgg ctagttgttt agacaattca
     9241 gttttaccaa ccccagtcgg tccaacgaat aagaaagaac caatagggcg atttggagtt
     9301 cctaatccca cacggttacg acgaatagcc ttagcgattt tatcaacagc atcgtcttgc
     9361 ccgatcacgt gtgctttcag atcattggct aagtttacga gttgagactg ttccttttct
     9421 tttaagtccc caactggaat attagttttt tgctctacaa tagcttcaat ggttttttca
     9481 gtaatgatgg gaatatcttg ctcatcgact ttttgagcct gcatttcttt gtattttgta
     9541 atttgatcgc ggaaataagc tgcgcgttca tagtcttcgt ctcgagtagc ttgcgccttg
     9601 agattctcag cttcaataag acgtttgtca atttctttag gatcaacaaa gttgagggtt
     9661 agattcattt tggaaccagc ttcgtccaga aggtcaatag ccttatcagg aagaaaacgg
     9721 tcttgaatat agcggttaga taaatgagcg gcagcttcaa tagcggctgg gctatatttg
     9781 acatgatgat agtcttcgta tttcggttgg atacctttta aaatcgtgat ggtttcctca
     9841 acagaaggtt catcaacttt aacgggttgc atacgtcgtt ctaaggcagc gtctttttca
     9901 ataatacggt attcatttaa tgtagtagca ccaacgagtt gcaactcacc acgggccaag
     9961 gctggtttta aaatattacc agcatccata ttgccgtctc ctgcagaacc agcaccgaca
    10021 atctcatgaa tttcatcaat aaagagaatc acatccttgc gattgcgaat ttcttccatt
    10081 aatttttgca tgcgctcttc aaactgacca cggatacctg ttccctgaac gaggctaacc
    10141 acatcaagac gaatcacttg cttgccttgg agtttttgag gaacagtacc atcaataatt
    10201 ttttgagcca aaccttctac aacagcagtt ttaccaactc caggctcacc aattagcaca
    10261 ggattatttt tagtacggcg gttgaggatc tcgataacgc gtgtaatctc ttcgtcacga
    10321 ccaataacag gatcaatatt accatttctt gcaatatctg tgacattaat cccaaactct
    10381 tctaataagc cctttgcttg ctgatttggt gtttgtggct gagcaggtcg ttgtccgttg
    10441 tagttaccac catagcgtcc tccgccattt ccattttgcc ctgcctgagt aggtggggta
    10501 tttggaagat taccaaaagc tctaaaatta ttcaaatcac caaaaaagtc atcaaagaaa
    10561 ggacttgtgg atctatcttg tgctctatat cctggggtta ggccatttaa aatagaattg
    10621 gcaggatcgg atttcatgat ttgataacaa ttttgacaga gatcaacctg tctttgtttt
    10681 ccattaacac ttgtataaag atgaatagta gattcgttta aattacaatt ttgacacagc
    10741 ataatatacc tccgagggtt tatctaaaag tcaaagtcat ttagtcaaaa atggtcaaac
    10801 tttatattca tattatagca ccacggcttg ggaaatcaag taaaaagttt atcgctttag
    10861 cgttagcttt tagagagtaa catgacagag taactttatt aagtttccaa tgagcaaatg
    10921 tttaaaatca ttgaaaagag taaaaaaagt aaaatatcaa taaatatatt actttccttg
    10981 ttagtttccc ttgttttgtg ttaaaataag agggataaaa aacgaatagg agaaacgaaa
    11041 tggaatcaca tttggtgaga atcatcaatc gtttggagtt gatggtgaca gatggcggta
    11101 atctcaagcg aaattttgaa cgcgatggtg ttgttgttgc agaagttgcc ttttcaaatg
    11161 atcctgaaaa tggacctgtt ttcacattgc gtgatgttga agcgcgtgag tcatattcct
    11221 ttgatagcat tgacttgatt gcaatggaaa tctacgattt attatattaa gcttgacata
    11281 gtaaagagac ttgcctgagg caggtttttt tattgtcaaa aagtattgta ttaatattca
    11341 tcagatggta taattatctt tattgtaatg aacctaggac ttatgaggag atataaatgg
    11401 atttgtcatt tttgcccaaa tactgggcct actttaacta cggtgtactt gtcaccatta
    11461 tgatttcagt cagcgttgtc ttttttggaa cccttattgg tgtcttggta accctgatta
    11521 agcgtagtca tgtgaagccg ttgacctggg tcgttaatct ttacgtttgg atctttcggg
    11581 gaacacctat ggtggttcaa atcatgattg cctttgcttg gatgcatttt aacaatatgc
    11641 ctactattgg ttttggggtt ttagacttgg acttttcaag actacttcct ggaattatta
    11701 tcatttcatt gaatagcggt gcttatattt cagaaattgt tagagcaggt attgaggctg
    11761 taccaaaagg gcaattagaa gcagcttatt cactaggtat tcgtcctcaa aatgccatgc
    11821 gttatgtgat tttgcctcag gcctttaaaa atattttgcc agccttagga aatgaattta
    11881 ttaccattat taaggatagt gcttttttac aaaccattgg agtgatggaa ctttggaatg
    11941 gtgcccaatc agtggtaacg gctacttatt ctccaatttc ccctttactg gtggctgctt
    12001 tttactactt aatggtcaca acagtgatgg cacagttatt ggcagtctta gaacgtcaca
    12061 tggcgcaagg aggtaatcat tgatgactga ggccattatt tcaattaaag acttgcacaa
    12121 gtattatggg cacaatgaag tgcttaaggg aattgattta gacattatgc caggagaggt
    12181 tgtggtgatt attggcccgt ctggttctgg aaaatcgacc ttacttcgaa ccatgaatct
    12241 tttggaagta ccaactaagg gacagattag gtttgagggg attgatatta ccgataagaa
    12301 gaacgatatt ttcagcatgc gtgaaaaaat ggggatggtt tttcagcagt ttaatctctt
    12361 tcccaatatg actgttttag aaaatatcac tttatcgcca atcaaaacta agggaatggc
    12421 taaagcagag gctgacaaaa cagccttgag cttgttggac aaagttggat tatcagaaaa
    12481 agccaaggct tatcctgcta gtctttctgg tgggcaacag cagcggattg cgattgcgcg
    12541 tggactggct atggatccag atgttttact ctttgatgaa ccaacttcag ctctagaccc
    12601 agaaatggtg ggtgaggtct tggctgtcat gcaagatttg gctaaatctg ggatgactat
    12661 ggttattgtg actcatgaaa tgggctttgc taaagaagtc gcagatcgtg tgatttttat
    12721 ggatggtggc gttattgtgg aagaaggaag tcctaaccaa ctttttgatc tgactaaaga
    12781 agaacgtaca aaagattttt tgagtagagt attataaatc tgttctatga attataaaag
    12841 gaagtcctaa atcatttagg acttcctttt ataatttgct aaggcatttt ataggagatt
    12901 acttttcttt ttgttaaaat aaaaagaaat agttacatta tttgcataga agaaagggat
    12961 ctgttatggt ttcagtgatg tggtttcgta gagatttaag gattgatgat caaaaagctt
    13021 tagctcatgc tattgctagt caatcgccaa ccttatgtgt gtttcaattt aacaaggagc
    13081 aattaaacct gctacatagt cggaatcaat ctagttttat tactagcgtt ttggctttta
    13141 gaaagttatt aaaatgtgaa agtattgact tatatctaat gtatggagac ttaatgacct
    13201 gttttgagca attattgaca caattaaaag actggacaga tgtgtatttt aactatgatg
    13261 aatctggata tggtcgttta cgtgatcaaa aagcagcaca atttttcaaa aaaaatggga
    13321 ttgctgttca tacctaccaa gatcactatt tacatggtag tcaagagatc attaaccaat
    13381 caggtcagcc ttataaagtt tttacgccct actataggat ttggcaaaat tatccgaaag
    13441 agactcctat taaagtagaa ctatcgcaag gcaggtggtt aaatcttgag acaccagatg
    13501 atgttttaag gactgttgag agctttaaag atgaaaaata tcaggatgtt gcaacttttg
    13561 atgaagcgtc aaaacaacta aatcgattta ttcaagacca attagcagct tatcacgcta
    13621 atcgagattt tccagcgcag ctagggacga gtcggttgtc tcccttttta agaataggag
    13681 ctattggcat tcgcacggta tatcacgctg tgcgtcaagc accaaatagt ctagggcaag
    13741 ctactttttt aaaagaatta gcttggagag acttttacaa tatggtctat gtggcttatc
    13801 ctgatcagaa aactcagcct atccaaaaag ccttttctca aatcgaatgg gttaataatc
    13861 ctgactggtt tcagttgtgg aaagaaggta aaacgggtta ccctattgtt gatgctgcca
    13921 tgcttcagtt acaaaagaca ggatggatgc ataatcgttt acgaatgata gtagcttctt
    13981 ttttaactaa agatctttta tgtgattggc gtttaggaga gcagtatttt caacaacaac
    14041 taattgatta tgatgctgcc agtaatatcg gaggatggca gtgggctgct tccactggaa
    14101 cagatgctgt tccttatttt agaatattta atcctgttac tcaaggtaag cgttttgatc
    14161 ccaaaggcga gtttattaaa gcgtatttgc ctcaactaga acacgttcca gagaagtatc
    14221 tccacgaacc gtggaagatg ccaaaatacc ttcaagagag tgtatcctgt attattggca
    14281 cagactatcc tcaaccaatt gtcgatcatg ctaaacaacg tgaacaagct attgcaaagt
    14341 atgagtgggc aaaagaaaaa gcaaaaatag aatgactgtt ctcattataa gagtttaaac
    14401 aaaaagttat catgaattaa gattatttaa tttaaaaaat aagttgactt tatttagatt
    14461 atttgatact attcagttgt ctcccttaga gaaagcgatt tcttgttttg gagctgattt
    14521 ttaccatttg gtcttagaat aggatgagta atatgactta caacgaggta tatcaagaat
    14581 ggttgcacaa taatgatctt agtgatgata ttaaagcaga tttagcagcc ataaaagaca
    14641 atgaggctga gattcaagat cgtttttaca aaacacttga atttggaaca gcagggctaa
    14701 gaggaaaact tggagcaggc accaatcgca tgaataccta tatggttggt aaggcagcac
    14761 aagctttagc taatactatt attgatcatg gacctgaagc ggttaaaaag ggcattgcgg
    14821 ttagttatga tgttcgctat caatctagaa catttgcaga attaacatgc tctattatgg
    14881 cagctaacgg tattaaggcg tacctttata aagggattcg gccaacacca atgtgttcgt
    14941 acgctattcg tgctttggga tgtatctctg gtgttatgat tacagctagt cacaatcccc
    15001 aagcttacaa tggttataaa gcttattggc aagaaggttc tcaaattttg gatgacattg
    15061 cggatcaaat tgcacaacac atggctgcac taactcagta tgaggaaatc aaacaaatgc
    15121 cttttgaaaa ggctctggac agtggacttg ttacttatat tgatgagagt attgaagaag
    15181 catataaaaa agaagtgctt ggtttaacga ttaatgatac tgacattgat aagtcagtcc
    15241 gagtagtcta taccccttta aatggtgttg ggaatctacc agtgcgtgag gttttaagac
    15301 gccgcggttt tgaaaatgtt tatgtggtac ctgagcagga aatgcccgat cctgatttta
    15361 caacagttgg ctatcctaat cccgaagtcc ctaaaacttt tgcatattca gaaaaattag
    15421 gtaaggcagt tgatgctgat attttgattg caactgatcc agattgtgac cgtgtggctt
    15481 tagaagttaa aaatgcagtc ggtgactatg tttttctcaa tggcaataaa atcggagcgc
    15541 ttttatctta ctatatcttt tcgcaacgat ttgacttagg caatttaccg gctaatcctg
    15601 ttttagtgaa atccattgta acaggagact tgtcacgggc tattgctagc cattatggta
    15661 ttgaaac
//
LOCUS       DAUVUY010000037        15629 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.037, whole genome shotgun sequence.
ACCESSION   DAUVUY010000037 DAUVUY010000000
VERSION     DAUVUY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15629
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.037"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(59..1096)
                     /locus_tag="VOX58_001037"
     CDS             complement(59..1096)
                     /locus_tag="VOX58_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HES5878269.1"
                     /translation="MLFMVNKKRRPLLKSHRHLLRLYTSLILLLVSLMGLALSLGESH
                     LSFLDLIHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(1057..2109)
                     /locus_tag="VOX58_001038"
     CDS             complement(1057..2109)
                     /locus_tag="VOX58_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HES5878270.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLANAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(2099..3031)
                     /locus_tag="VOX58_001039"
     CDS             complement(2099..3031)
                     /locus_tag="VOX58_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HES5878271.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(3057..3839)
                     /locus_tag="VOX58_001040"
     CDS             complement(3057..3839)
                     /locus_tag="VOX58_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878272.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(4085..5530)
                     /locus_tag="VOX58_001041"
     CDS             complement(4085..5530)
                     /locus_tag="VOX58_001041"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HES5878273.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMV
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIARHYL"
     gene            5618..7252
                     /locus_tag="VOX58_001042"
     CDS             5618..7252
                     /locus_tag="VOX58_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HES5878274.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSAIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFLIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLAAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLMALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYVFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            7420..8049
                     /gene="upp"
                     /locus_tag="VOX58_001043"
     CDS             7420..8049
                     /gene="upp"
                     /locus_tag="VOX58_001043"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HES5878275.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            8273..8863
                     /locus_tag="VOX58_001044"
     CDS             8273..8863
                     /locus_tag="VOX58_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002274529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HES5878276.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLTYGFIDEIMENNELK"
     gene            9358..9633
                     /locus_tag="VOX58_001045"
     CDS             9358..9633
                     /locus_tag="VOX58_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HES5878277.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIVYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKG"
     gene            9882..10517
                     /locus_tag="VOX58_001046"
     CDS             9882..10517
                     /locus_tag="VOX58_001046"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HES5878278.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            10535..11410
                     /locus_tag="VOX58_001047"
     CDS             10535..11410
                     /locus_tag="VOX58_001047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HES5878279.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHVNAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            11913..12236
                     /gene="yabA"
                     /locus_tag="VOX58_001048"
     CDS             11913..12236
                     /gene="yabA"
                     /locus_tag="VOX58_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HES5878280.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            12241..13104
                     /gene="rsmI"
                     /locus_tag="VOX58_001049"
     CDS             12241..13104
                     /gene="rsmI"
                     /locus_tag="VOX58_001049"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HES5878281.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYL
                     EETPLKGECLLIVAGAQVDSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            13131..13523
                     /locus_tag="VOX58_001050"
     CDS             13131..13523
                     /locus_tag="VOX58_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878282.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(13570..14199)
                     /locus_tag="VOX58_001051"
     CDS             complement(13570..14199)
                     /locus_tag="VOX58_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HES5878283.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTSENQ
                     LDTDAIEQLLPATQGLPLVFHMAFDRIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQSLSQLTGTAIVHGTKII"
     gene            14498..14854
                     /locus_tag="VOX58_001052"
     CDS             14498..14854
                     /locus_tag="VOX58_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HES5878284.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGN
                     VLQVGYRKPYQELDLG"
     gene            complement(14928..>15629)
                     /locus_tag="VOX58_001053"
     CDS             complement(14928..>15629)
                     /locus_tag="VOX58_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HES5878285.1"
                     /translation="QETKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTM
                     FLYKNTLNPVITFPEIGAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQI
                     WDHKYADYLTELDAQKPVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLA
                     RGFTDTFRHLHGDIPHVYTWWAQRSKTSKINNTGWRIDYWLASNRIVDKVKRSEMISS
                     GERQDHTPILLDIDL"
BASE COUNT         4899 a         2986 c         3212 g         4532 t
ORIGIN      
        1 ttatagcacc atagaaaaga gctaggaaca aggactgttc ttagcttttt gagtgctttt
       61 attttgtttt tgccattaag taaagaaaat aaggggctcc gataagcgac actaaaatcc
      121 ctgtctggat attactgcca accagataga ctcgtcctac tgtatctgca acaagaagta
      181 ggatcattcc gattaaaata ctaatgggta tcgttatttt atgattactc cctaccaagt
      241 atgtactaaa gtgtccagct attaagccga taaaactaat attaccaacc aaaagaacac
      301 tcaaagcacc taagccagct gctaagacaa gtatcaaacg acgcttacgg tttaaaggga
      361 gtcctagccc aatagcagta ttatcagcta atcccataat attaaggaaa tgggcttgac
      421 tataagtcaa tagccaaaaa cacaataata aaggagcgat gacactcaga gtaggccagt
      481 cgtctcctat taattggccg ctaagccaat tgatgaccaa atcgactttg tagcgattaa
      541 tatgaccaac aagagcaacc attagacttg aaagcatggt agtaacagct acaccagtaa
      601 gaattaaccg tgtaggatca atttgtcctt gttttgtcaa agatagccaa taaacgctaa
      661 aggttgttac aacagcacca agcatggcaa ataaaggcaa gtaatggctg atatgagctt
      721 gctcaaagaa agaaaaggag accacaatag ccaaagagat acctgctcct atagtaatac
      781 ctaatacacc agagtcagct aagggatttc gtgttaaacg ttgtaataat agccctgata
      841 aagcaagaga accgcctcct agacaagcag ctagaattct tggcagacga atgttaataa
      901 cgatgaaaga tatggcatgg ctagattttc ccaaaaacac atggataagg tccaaaaaag
      961 atagatgaga ttctccaagg gacaaagcta atcccataag acttacaagt agcaggatca
     1021 ggctagtata taaccttaaa aggtgtcggt gagatttcaa tagcggcctc ctcttcttat
     1081 taaccataaa aagcatggaa aaccaattaa gctaaccaat gccccaagag gggtttcata
     1141 aggcggattg agattacgac aagcaatatc aacccatacc atgaaactag cgccagaaac
     1201 tgcacaaagc ggcaaaaggt atcggtaatg atgaggtgta aagtgtttca taagatgagg
     1261 aatgactaag ccgataaaag aaatgcttcc agcaatggct acagctgctg atgataaaat
     1321 aagaaccaaa atcataaaga ctgcactgat caagttggtc ttttgcccaa gagcttttgc
     1381 ctgagactca cttagactaa ggacggtcag gtgataagat aatagctggg ctaaacaaag
     1441 actaagaata ataagaggag caatatagcc gatcatttgc caattgaccc cgacaagacc
     1501 tcctgcttgc cagccgataa cagcatttgc cagatgatag taattggtaa taccttgacc
     1561 taatgctgat agcagtatgg aaaccatagc tcctgctaag acaaggcgga gctgatggta
     1621 gcctttgcca gattgataag aaaggccaaa gactaaggtt gctgccaggc tagaccctaa
     1681 caacgaaagc agaataatga gggaatagtg caagtgaggt acaaacgcat aagccagtac
     1741 caaggctagt cctgctccag cattaatgcc taaaaggcca ggctcagcga taggattacg
     1801 tgtgattgct tgcatgatag ttccagaaac agctaacgca gagccagtta aggtagcacc
     1861 aaaaagtctc ggtaatcgta tagcgagtaa aacattggct ttatggccgt tttgctttcc
     1921 aaataaaata gagttaagat cctgatgtga taaagcaacg gctccaaagc gcagatgaca
     1981 gtaaatagca acacataagc taaaactaag tagcacaaag acaagccaaa agatatgtga
     2041 cttagtgatt atctgcgttt tttttaagca aaaacgctcg agacacaaaa agggatgctt
     2101 agttttcact tgataagatt gcctctcgta aagtctctaa ttgatattct aaagaaagag
     2161 gatcagtaaa gtaaaaaaca ttggcattga ctttgatcac atggtgtttt ttcacagctg
     2221 gaatggattg ccatagctta ctttcatata gagcagaacc agtcttatca tcttctgcag
     2281 caacgacgac ataatcacca atatagtctg gcaagacttc ctgcgacaat gacaggtaac
     2341 cttgtttaaa aacttcagtc ttcacttttt caggagcatc ataatggaaa gcttgatgga
     2401 taacctctcc gccacgtccc cagtctttac caaaaaggta aacgtctttt tcataaagcc
     2461 ccataatagt aaaggttgct ttgtcacctg taacggcttt gacttctttt tcataagctg
     2521 ctgttttagt tttccaatct tttaaccatt tcttggcttt gccttctttg ttaaagatgc
     2581 ggccgaagtc agataacact tgtaaataat cacgtttgcg gtattcaatc gaaataacag
     2641 gcgcaatttc tgccaattgt ttaatatttt cttctgtaga gccaacaaca atgaggtcag
     2701 gcttaagcgt tgtaatggct tctaaatcag ttgcagcaac ttgctttgct ttttttacag
     2761 tctttgctaa gatggggttt ttcttgtcat aagaagtaac cccgactaga ttcatatcta
     2821 attttttgag gtaaccagtg taagttgaag ccagactgac tactcgctta ggctgtttag
     2881 gaatatctcc gtaataagtc acaccagcaa tttgaggcat aggcgataaa ctttttgagg
     2941 cggtgttaga atgattagtt gcttgattac cacaggcgat taaggtaata gttgtcaaac
     3001 agagtgttaa tagtagagtt aattttttca tgtatgctaa ttctcctaga ataaagttat
     3061 tgaagctgat aagtcagcac tataggacag ttgtggatag gatcatcaac cagtaccggt
     3121 ttgatttgga agatgtcttg aatgataggt gaagtcataa catctgctat agtaccagta
     3181 taatggatct tgccatgttt catagcaatc agatgatccg aatagcgggc tgaaagattg
     3241 aggtcgtgaa gaaccataac aatagtcttg ccggcatcct tatttaggga tttaacgagc
     3301 tctaggattt ctagttgatg atttaaatct aaataagtag ttggctcatc taaaaaaata
     3361 gtatcagtgc cttgggctaa tgccattgca agccaaaccc tttgacgttg ccccccagaa
     3421 agagcatcaa caggttgatc agcataggcc atgacattgg tggcttgcat agcccaatga
     3481 atggcttttt tgtcagctgg actaagattt ccaaagtaag attgatgggg aaaacgtccg
     3541 taagaaacca attcgtaaac agttatgccg ttagttgctt cttgaacttg aggtaaaaga
     3601 gctagttttt tagcaacctc tttagtttct aatgtggcaa tgttttgccc atttaaatat
     3661 acaactccct gttttggtgg taataatctt gtcagtgctt ttaataaact agacttccca
     3721 cagccattgg cgccaataat ggtcgtaatt ttaccttcag gaatataaaa cgataattta
     3781 tcgatgatgg tacgctgttc ataggcaatt gtgaggtctt cagcactaat tgttgtcata
     3841 tggttgtctc ctttctaaaa agagtcaaag ggttgatgaa agtgcacatt aaagtattca
     3901 tctgcccatt ttaaaatagt tgagaagtag cttattttct caaaatcttc actattatag
     3961 catagaaaaa aggcttaaca aagccttttt tgatgtgatt tttaggatgt attaaaaaag
     4021 aaatattagt ttagaatgat taaaaacaat agcgataatc attctaaact aacgtcaaca
     4081 tactctagag gtagtgtctg gcgattgcta aatcaccagc ataagctact tgttgtcctt
     4141 taacaatttg ataagcatct gcgcctttcc cagcgataat cacagcatct tttgctcctt
     4201 ggcaaagaga catggctttt tgaatagctt gctcacggtc actaataatc tctactgggc
     4261 gtgcgatatg gctggcaatt tcttttgaaa tatcttctgg atcctcgaaa ttagggtcat
     4321 cagcagtgag aatgacggtg aggtttggat gctgatgaat caccctacca aaatcagctc
     4381 gtctgctctc gcctttattt cctggtgcac ctaaaatcaa cattaatttg cctgtctgat
     4441 gttcttccac aacgctgagt aatttttcta agctgtcacc attatgggca taatcaacaa
     4501 aaactttagc atgatttgtc atagtgagga cttccatacg gcctgggacg cgagtcttag
     4561 cgataccttt ttgaatatca gctaggctag cacccaaacg aaggcaggca agtccagctg
     4621 ccatagcatt ttcttggtta aaatggccaa ttaattgaat gtcataatgg ccagctaatt
     4681 gtcctttagc ttcaaaggag aaggcttggc tagtggtgat ctggttgtca gacaagggac
     4741 cataaaacac atgctcttga tccgctacct gatccgctag aaatgaaaaa tgatccatcc
     4801 cgctattgat cacaacagct ctgctattct ccattaaaag acgcttatga tagaaataat
     4861 cttcaaacgt tggatgctcg attgggccaa tatgatcagg actaatattt aggaaaaccc
     4921 caacatcgaa cgtaagccca tagacacggt caacgaggta agcctgacta gaaacttcca
     4981 taatcaagtg ggtcatgcca ttagtcacac actcagccat catagcaaaa agatccaagc
     5041 tctcaggagt tgtgagttgt gatttgaaaa aggtcttgcc atctaaggtg gtattcatgg
     5101 tagaaaacat ggcaggttta taggattctt tcaacatgtg gtaggcaaaa taagcagccg
     5161 tcgtctttcc cttagtacct gtaaaagcta gtagtttgag cttttcttga gggttgccat
     5221 agaaaaccat ggcaattaga ctcatagctt ttttgatatc agtgaccaaa acaacgggaa
     5281 taccaagctc gtaatctacc tctgaaatat agagttgtaa tccgttagtg attgcttctt
     5341 taaggtagtc agctttgaaa gttgcccctt tagcaaaaaa aagggtcttg ccatctactt
     5401 gacggctatc gtaactcaag cgctcaaaac taaagccttg ataatggtag tggtatccgt
     5461 cggcatcaag aacctcacga aaattatggt ctttttttaa aatgtctaat aattgttcaa
     5521 tggttatcat acccttattt tatccttaaa gttcttgttt tacaagtcac aagcaaaagt
     5581 ttataataaa gctaatagaa actaggaaag aatagatatg tcgacagaaa aaaaacaact
     5641 gacacaagaa gaattgatgg ttcagggagc agcctggtcc acagctggca atttcattag
     5701 ccgacttttg ggagtgttat acattattcc ctggtatatt tggatggggc aatacgcgat
     5761 tcaagccaac gctcttttca atatggggta taatgtttat gcttattttt tattgatttc
     5821 aaccacagga ctcaatgttg cgattgctaa gcaggttgcc aagtacaatt ccatgggaca
     5881 aactgagcat agctatcaat taatccgaag taccctaaaa ctcatgcttg gtctgggcct
     5941 tattttttca gccattatgt atctgggctc tcctttattt gcgagcttat ctggtggtga
     6001 tgacacactt gttcccatta tgcacagcct ttctttagca gtttttattt ttcctgtaat
     6061 gagtgtcatt cgaggtatct ttcaaggcca caataatatc aagccttatg cgattagtca
     6121 gattgctgag caactcattc gggttatctg gatgcttttg accaccttcc ttatcatgaa
     6181 attaggttct ggtgattacg cttcagcagt aactcaatca acttttgcag cctttatcgg
     6241 gatggtggct agtatgggtg ttctggggta ctatctttgg aaacagggac ttctagcagc
     6301 tatttttagt aagccagatc atactgtttc cattgatatc aagggcttgt tgcttgaaac
     6361 cttaaaagaa tccatcccct ttatcgtaac aggaagtgcc attcaagcct ttcaattgat
     6421 tgatcaatgg acttttgtca atactatgac tctttttaca gattatagcc gttctcaatt
     6481 gcttgtttta tttggttact ttaacgcaaa cccagccaaa attacaatgg ttttaattgc
     6541 agtagcagca tctattggtg gtgtaggcat tgccttatta actgaaaatt atgtcaaaaa
     6601 agacatgaaa gcagctgctc gcttaatcat taacaacatt gaaatgttag taatgttttt
     6661 gttacccgct cttactgggg caattatttt agcaagacct ctatattctg ttttttacgg
     6721 agctagcgag gatcgtgcca ttcacctctt tgtggcggtc ctcttccaaa ccttgttgat
     6781 ggcgctttac accctttttt caccgatgct tcaagctctt tttgaaaatc gaaaagcgat
     6841 ttactacttt gcctatggta tcttgattaa gttagtttta cagataccgc ttatttattt
     6901 gctacatgct tatggtcctt tactagcgac gacgattgct ttagtggtgc cgatttattt
     6961 gatgtatcga cgtctatatc aggttactca ttttaaccgc aaactgttgc aaaaacgttt
     7021 attattaacc ttgattgaaa ccttattaat gggactggtc gtgtttgtgg ccaactggct
     7081 attgggctat gtctttaaac cgacaggccg cttgaccagc cttctttacc tcctcattat
     7141 tggtggcttg ggaatgacgg tttacacggc actcaccttg ctgacgcatc aactggataa
     7201 attaattggt agcaaagcta gtcgccttcg tcagaaatta ggctggcatt aatataatga
     7261 caccaagaac gatttcgtga gtgcttatcg aaatcgtttt tttcttaatg atctcgctta
     7321 gtaaaggtag ccgataaaaa atcaggattt cccaagcaaa ctactgatat ttttggtata
     7381 atgataaaag tgtaaaaaaa tagaaggaag tatatcaaca tgggaaaatg tcaagttatt
     7441 tcgcatccgc ttatccaaca caaactttca atcttacgtc gtcaaaccac atcaactaaa
     7501 gattttcgtg agttggttaa tgaaattgcg atgctgatgg gatatgaagt gtcacgcgac
     7561 cttcccttag aagatgtcga catccaaacg ccagtatcaa aaacggtcca aaaacagtta
     7621 gctggtaaaa aattagctat tgttccgatt cttcgtgctg gtattgggat ggttgatggc
     7681 ttgttaagtt tggtaccagc tgcaaaagtt ggccatatcg gcatgtaccg taatgaagaa
     7741 actcttgaac ctgttgaata cctcgttaaa ctaccagaag atattaacca acgacaaatt
     7801 ttccttgttg atccaatgtt ggcaacaggt ggttcggcta tcttagcagt ggattcgttg
     7861 aaaaaacgtg gtgcagcaaa tattaagttt gtctgtttag ttgcagcacc agaaggggtg
     7921 aaaaaacttc aagaagctca tccagatatt gatatcttca cagcagcact tgatgatcat
     7981 ttaaatgaac atggctatat cgtgccagga ttgggagatg ctggtgatcg tttatttggg
     8041 acaaaataaa agagacaacc atcaaatagt agccttttta ggccaatcgt tcttgatttt
     8101 gttaaaaaaa tcagagagat ttctcttctt taaaggaaaa gcaagtttat gatttgacct
     8161 ttattgacca aaatattata ataaagacaa tcagtaaaaa agagatgacc tagtgggttg
     8221 ccgtctctca aaatgacgct actaaaaacc cttgtagatt ggagaatgta ttatgattcc
     8281 tgttgttatt gaacaaacta gccgtggaga acgttcttat gacatttatt cacgtttatt
     8341 aaaagaccgt attatcatgt taacagggcc tgtcgaagac aacatggcca actctgtcat
     8401 agcgcagctc ttattccttg acgcgcaaga caacacgaaa gatatctacc tatatgtcaa
     8461 cacaccaggt ggctctgtct cagcaggctt ggctattgtc gataccatga actttatcaa
     8521 ggccgatgtg caaactattg tcatgggtat ggcagcttct atggggacag tcattgcttc
     8581 atcaggaaca aaaggcaaac gctttatgtt accaaatgca gagtatatga tccaccagcc
     8641 aatgggtggc acaggcggtg gcacgcaaca aacggatatg gctatcgcag cagaacatct
     8701 cttaaaaaca cgtcaccgtt tagaaaaaat cttagcacaa aatgctggga aaacgattaa
     8761 acaaattcat aaagatgctg agcgtgatta ttggatgagt gctgaagaaa ccttgactta
     8821 cggtttcatt gatgaaatca tggaaaacaa cgaactaaaa taaagaggat atctgcaaaa
     8881 gtattctaga aatttctagg atgctttttc tcttagaaag gaaaataatg atacagccta
     8941 ctgcattaaa acagcttcaa gagttaaaac gtcagttcag atccctttgc aaccaattat
     9001 gagatagttt gaggatgatc cttagcagct gtgtgcaaga agttatgacg atttcataaa
     9061 ggatgaccta gccagtcttt cttcgtctct tttacttaaa gtgtggtata atcagattat
     9121 gctagccaaa attaaaaaag gcattgccat taaaggtgtt gtagatcggc ttatcctaga
     9181 tctcataaca atgctgtagt accgccctta ccttgctact aaaggcgctg ttgaagcaca
     9241 aattgacttg caaaggatct tgactgatag gtatatgtct ggtggaatcg ccaaagcagc
     9301 aaaaagacca agacccttat ttggtctcta ccaaaatata atagaaaagg atttagaatg
     9361 tttcaaaaac aagaacgtat tggactcgtc gtttacctgt attataaccg agatgccaga
     9421 aagttatcaa aatttggtga tttatattac cattccaagc gctctcgtta tctcattgtt
     9481 tatattaata aaaatgattt agacacaaaa ttagaagaaa tgagacgttt gaaatgtgtt
     9541 aaagacatta gaccatcagc ttttgatgat attgaccgcc aatttgtagg taaccttcat
     9601 cgcgacgaaa caaacaacca tcaaaagggt taacaacccc ctagttactg attagcgttt
     9661 tagatttagt tagcttatta aagtaagaag gttttgacag atgctcacac tacttcaaga
     9721 caatgagaca actagagcat tagtgaataa ctgagttgac ggtcacttct ttaataagaa
     9781 cggataggat tggtcctaaa atagagcact gcaaagatat gccagtgctt tttagctcat
     9841 tttttgataa aatagaacta ctaaaaagtg aaggaaataa gatgatcaca ggaaaactca
     9901 taaccgttga aggtccagat ggcgcaggga aaacgactgt tttagagcaa ctcattccac
     9961 tgttaaaaca aaaagtggct caggagatct tgacaacaag agagcctgga ggtgttgcca
    10021 tttcagaata cattagagaa cttattttag acatcaacca tactactatg gaccctaaaa
    10081 cagaactctt actttatatt gcggctcgaa ggcagcattt ggtggaaaag gtactaccag
    10141 ctcttgaagc aggccagttg gtttttattg atcgtttcat cgatagttca gtagcttatc
    10201 aaggtgcagg acgcggtttg atcaaagcag acattcagtg gctcaacgag ttcgcaacag
    10261 atggtttaga gcctgacttg accctttatt ttgatgttcc ttctgaaatt ggtttagcac
    10321 ggattaacgc gaaccaacaa agagaagtca atcgtttaga tttggaaaca atagagatac
    10381 atcaacgagt acgaaaagga tacctagcct tagctaagga gcatccaaaa cgtattgtca
    10441 caattgatgc gactaaacca ttgaaagaag ttgtctctgt cgctttggaa catgtcttag
    10501 ccttgctttt agcctaggat aagggagggg aggaatggat ttagcgcaaa aagctcctaa
    10561 cgtttatcaa gcttttcaga cgattttaaa gaaagaccgt ctgaatcatg cttatctttt
    10621 ttcgggtgat tttgctaatg aagaaatggc tcttttttta gctaaggtca tcttttgtga
    10681 gcagaaaaag gatcagacgc cctgcgggca ttgtcgctct tgtcaattga ttgaacaagg
    10741 agattttgcc gatgtgacgg tattggaacc aacagggcaa gtgattaaaa cggatgtggt
    10801 caaagaaatg atggccaact tttctcagac aggatatgaa aacaaacgac aagtttttat
    10861 tatcaaagat tgtgacaaaa tgcatgtcaa tgccgctaat agcttgctaa agtatattga
    10921 ggagcctcag ggagaagctt acatattttt attgaccaat gatgataaca aagtgcttcc
    10981 gaccattaaa agtcggacac aggtttttca gtttcctaaa aacgaagcct atctttacca
    11041 attggcacaa gaaaagggat tattaaacca tcaggctaag ctagtagcca aacttgccac
    11101 aaataccagt catctagaac gtctgttgca aacgagcaag cttttagaac tgataactca
    11161 agcagagcgt tttgtatcta tttggctgaa agatcagttg caggcatatt tagcgttgaa
    11221 ccgtctggta cagttagcaa ctgaaaaaga agaacaagat ttagttttga cccttttgac
    11281 cttgctcttg gcaagagagc gtgcgcaaac gcctttgaca caattggagg ctgtctatca
    11341 ggctaggctc atgtggcaga gcaatgttaa ttttcaaaac acattagaat atatggtgat
    11401 gtcagaataa gtgcaattgg aattcgttat ggggaaacga agcttgtcaa aagacttgcc
    11461 cgtactccct tttcagcaga agagcacatg gatttttaca actgttaaaa gatttagcgg
    11521 ccgattttag gtcaagaagt gagctgcccc agattaacag ccaagaagat ttcattttct
    11581 atggaggcta tggaccgtgt ggcaggccac tgtgctgcat tcgtttttgg gaaaatttct
    11641 gacagtatct attaaaatac taaaaaatca agggttatca ttgactttgg gtaaagcaac
    11701 aagcccttat ggccgtttgc tttgctgtct ctatgatgaa ggtggctttt accaataaag
    11761 caagaaaaaa ttccctgaat atggtaccat agtagagaca agtgatggcc ctgcaacagt
    11821 agcagccgtt gatatattta cagatcccat taaggtgcgg ttaaaagacc agttgacctt
    11881 agtcacttat gctttagagg aggttaaagt aggtgaataa aaaagaatta ttcgatgctt
    11941 ttgatggatt ttcccaaaat ttaatggtta ctttagctga aattgaagcc atgaaaaaac
    12001 aagtccaatc actagtagaa gaaaacacga ttcttcgcct agaaaataca aaactacggg
    12061 aacgtttgag tcacctagaa catgaaacgg tagctaaaaa tccttccaaa caaaggaagg
    12121 atcacttaga gggaatttat gatgaagggt ttcatatctg caattttttc tatggacaac
    12181 gtcgtgaaaa tgatgaagaa tgtatgtttt gcagagaatt attagataga aagtaagggc
    12241 atgcaggttc aaaaaagttt taaagacaaa aaaacaagcg gaaccttgta tttggttcca
    12301 accccaattg gcaatctgca ggacatgacc tttagagcag tggcaacgtt aaaagaggtt
    12361 gattttattt gtgcagaaga cacaagaaac acaggtcttt tgctaaaaca ctttgacatt
    12421 gctaccaagc aaatcagctt tcatgagcac aacgcctatg agaagattcc tgatttaatt
    12481 gatttattaa tatcaggacg gtcactagct caggtgtctg atgcaggaat gccatcgatt
    12541 tctgatcctg ggcatgattt ggtcaaggct gctattgata gtgatatcgc agtcgttgct
    12601 ttaccaggtg cctcagctgg gattacagct ttgattgcta gtggactggc tcctcaaccc
    12661 catgtttttt atgggttttt acccagaaaa gcagggcaac aaaaggcttt ctttgaggac
    12721 aaacaccatt acccagaaac acaaatgttt tatgaatcgc cctatcgcat caaagatacc
    12781 ctgaccaata tgttggcttg ttatggtgat cgtcaagtgg tgcttgtcag agaattaacc
    12841 aagctttttg aggagtacca acgggggtcg atttcagaaa tcttgtccta ccttgaggaa
    12901 acacccctca aaggggagtg tctgctgatt gtggctggag cccaagtaga cagtgaggtt
    12961 gagctgacag ctgatgttga cttagtgtct ctagtgcaaa aagaaattca agcaggagcc
    13021 aagccaaatc aggctattaa aacaattgct aaagcttacc aggtaaatcg ccaagagctt
    13081 taccaacaat ttcatgactt ataagtcatc gtaggttagg agaaaaacaa atgacagaag
    13141 atcaagcaac tatccttatt ttaggagtat acggaatcat attggctgta ttcttattat
    13201 cagctttatt atgccttatc gctaactggc gactctttaa aagagcaggt tacaaaggat
    13261 ggtatagtct cattcctttc tatgcaggtt ttgtcaaaca caaaatcact tttggagaag
    13321 ataataaatg gttttacttt attggttggg tcattgctat ttattattac tatgtgcact
    13381 tttgctatgt ccgtgctttt ggtggatcaa agggtctagc agtatttggc ttgtttttcc
    13441 caggcattgc ttctttgata gtggcttttg gaaaaactta taccacacaa gaatgccacc
    13501 ctgtgccaca tcttttaaac taagagaaaa aagaccatca attggattta ttgatggtct
    13561 tttaggtgtt taaatgattt tagtcccgtg aacaatggcc gttccagtga gttgactcaa
    13621 gctttggcaa ttctcagcag tgacgcctcc cccaatcatg atttcaatgc gtttattggc
    13681 ataggtcaca agacttttaa gctgttccac attgtctgta ataggagtag cttcaggtga
    13741 gccatgagtt agcacacgca caaagccata atcaatcagt tggtcaagcg cttggtgctg
    13801 gtgatcagtc ggaatccggt caaaagccat gtggaaaacc agtggcaagc cttgtgtagc
    13861 aggcagtaat tgctcgatag cgtcggtatc cagttgattt tctgaggtca gcaagcctaa
    13921 gactagcgca tctgagccag cttcaacagc ttttaagata tcttcttcca tagcttttag
    13981 ttcgaggtcg ttatagacaa aatcgccacc gcgaggtcga atcatggtcg ctacgctaat
    14041 ctttttatcg tgtaagagct gacaggcttc tttgatgacg ccataagatg gcgtggtccc
    14101 tccgacagct aggttatcac agagttccac acgactgact tgaccagcat caagggttgg
    14161 cagtagtgtg aggttctcag cacaaaattc tttaatcatt tttagttctc ctttatcact
    14221 aatattatag cacgaatgaa gctagtcatc aggaaacatt tttgtcaaat catggacatt
    14281 atgctatact cactagtatt aaaagtaggc caaaacagca gcaaatactt tgaaaaaaga
    14341 ttgctatgtt ctacggcaat atcagtcttt accaacgaac tctcaagaag aaaagaaaga
    14401 caaagcttag cattttaaaa aagcccccct ttttttgtga aatgatgacc aaaaacaggc
    14461 gcctcacgcc taaaacagtt accctaaagg agaacaaatg attacgattt accaataccc
    14521 aaaatgtagc acctgccaaa gagccatggc tgaattaaag caactggtta gtgactttga
    14581 agccattgac atcaaagcta atccacccaa agctcaggac ctcaaacatt ggatggaaac
    14641 atctggttat accatcaaaa atttctttaa taccagtggt aacagctacc gcgaattggg
    14701 acttaaagat aaaatagacc agcttagcct tgataaagct gctgaacttt tagccacaga
    14761 tggtatgttg attaaacggc ctattctgat taaggacggt aacgtcttac aagttggcta
    14821 tcgcaagcca tatcaagagt tggatttagg ataatgcgac aaagtctcaa aaaaggacaa
    14881 ggcaacatta taacgccttg tccttttttg ggatctgttt ttagacatta gagatcaata
    14941 tctagtaaaa ttggcgtatg gtcttggcgt tccccagaag aaatcatttc agaacgctta
    15001 accttatcca cgattcggtt tgaagctagc caatagtcaa ttctccaacc ggtattattg
    15061 attttgctgg ttttactacg ttgtgcccac caggtataaa catgaggaat atcgccgtgc
    15121 aagtgacgaa aagtatctgt aaagcctctt gctaataggt tagtaaagcc ttgacgttct
    15181 tcgtcggtaa aaccaggaga acgtcgattg ctgcttggat ttgccaaatc aatctcttta
    15241 tgggcaacgt tgtaatctcc cgtagccaaa acaggctttt gagcatccag ttcagtcaaa
    15301 taatcagcgt atttgtggtc ccaaatttga cggtcatcta accgtcttag accatcacca
    15361 gcattaggcg tataaacctg tgtgacgaaa aagtcttcaa attctagggt aatgatacgg
    15421 ccctcagcat ccattgttgt tggggcacca atttctggaa aagtaatgac agggtttaac
    15481 gtgtttttgt acaggaacat ggtgccagca tagcctttcc tagcaggctc aactgaagaa
    15541 cgccacacat gcaaatagtt tggaaagtaa ctaagtaggg tctcaatatg ttttttggta
    15601 ggccccttag cagataattt agtttcttg
//
LOCUS       DAUVUY010000038        15482 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.038, whole genome shotgun sequence.
ACCESSION   DAUVUY010000038 DAUVUY010000000
VERSION     DAUVUY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15482
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.038"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..591
                     /locus_tag="VOX58_001054"
     CDS             <1..591
                     /locus_tag="VOX58_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HES5878286.1"
                     /translation="KTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTLFLKTP
                     QEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFTVSFLI
                     VIAMVWLTITYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGITLMTW
                     QYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            581..2305
                     /locus_tag="VOX58_001055"
     CDS             581..2305
                     /locus_tag="VOX58_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HES5878287.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQLQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRHDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDV"
     gene            2305..3789
                     /locus_tag="VOX58_001056"
     CDS             2305..3789
                     /locus_tag="VOX58_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5878288.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFDVVSTARSGQEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTNHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDWLDAQQAESIRQETYHDSLLTLWLTDELSEKEFHQLSQD
                     LPEHALTGFTVLYVECSEWIEEMEAYFKQEEQPCYLKKEEDKKIYLAILLGRAQQAKA
                     FTSNLQKQFSENIQQIILGETVDDWENVYESYNQVRQSLFYNSQMSQPTDKGNSKLEM
                     PESRLQFFAFNKALMIGDEPTILAKLEAIFHEMKALNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHNSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            3719..7225
                     /locus_tag="VOX58_001057"
     CDS             3719..7225
                     /locus_tag="VOX58_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HES5878289.1"
                     /translation="MVYRQKSSEIVTRTITKPSRATSNVKQEIDMTPDSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQDPSFDDSNWQVIHLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFMANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENASYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYANGKDYDKLHEEHSDWLLYGSET
                     ASAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNVTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSRGELVSHAQDWLKVQVEGPAHLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKVSQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKVSTPHILITSYQHY
                     LSLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSFVKKAFPMTWYLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLATSQKRLTYCYQYDTVQAIGA
                     ISLQNHTDTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLVVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            7363..8241
                     /locus_tag="VOX58_001058"
     CDS             7363..8241
                     /locus_tag="VOX58_001058"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HES5878290.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEIRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            8493..9248
                     /locus_tag="VOX58_001059"
     CDS             8493..9248
                     /locus_tag="VOX58_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HES5878291.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVTVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAKAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            9301..9639
                     /locus_tag="VOX58_001060"
     CDS             9301..9639
                     /locus_tag="VOX58_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HES5878292.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAAIPHALYAVEAFQ
                     MLLVVVKPEA"
     gene            9641..9946
                     /locus_tag="VOX58_001061"
     CDS             9641..9946
                     /locus_tag="VOX58_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HES5878293.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPRDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKAHHLEAKTDCKLLQVLLD"
     gene            complement(10381..11454)
                     /locus_tag="VOX58_001062"
     CDS             complement(10381..11454)
                     /locus_tag="VOX58_001062"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HES5878294.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKRIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(11510..12262)
                     /locus_tag="VOX58_001063"
     CDS             complement(11510..12262)
                     /locus_tag="VOX58_001063"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HES5878295.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(12780..13973)
                     /locus_tag="VOX58_001064"
     CDS             complement(12780..13973)
                     /locus_tag="VOX58_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HES5878296.1"
                     /translation="MIDSLLLKSLHSLLGLTITVCDQNFSVIREYKSDKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNAIDPLLLKKKLTETQI
                     NASEQDYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     LDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEHFSNSIVPIISGDELR
                     SEKNYSIIIYDRLSQATIQAGLDIETAYRARDRFIKETESTISLNEVLKLRDTAILFY
                     TQQVDSLKRHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEK
                     HITIQQYFINLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQ
                     KLKTI"
     gene            14213..>15482
                     /gene="spr28"
                     /locus_tag="VOX58_001065"
     CDS             14213..>15482
                     /gene="spr28"
                     /locus_tag="VOX58_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115157939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin R28"
                     /protein_id="HES5878297.1"
                     /translation="MFRRSKNNSYDTSQTKQRFSIKKFKFGAASVLIGLSFLGGVTQG
                     NLNIFEESIVAASTIPGSAATLNTSITKNIQNGNAYIDLYDVKNGLIDPQNLIVLNPS
                     SYSANYYIKQGAKYYSNPSEITTTGSATITFNILDETGNPHKKADGQIDIVSVNLTIY
                     DSTALRNRIDEVINNANDPKWSDGSRDEVLTGLEKIKKDIDNNPKTQIDIDNKINEVN
                     EIEKLLVVSLPDKIKYSPEAKHRTVEQHAELDAKDSIANTDELPSNSTYNWKNGHKPD
                     TSTSGEKDGIVEVHYPDGTVDDVNVKVTVTSKKTDNTAPTLTVTPEQQTVKVDEDITF
                     TVTVEDENEVELGLDDLKAKYENDIIGARVKIKYLTKEPNKKVMEVTIMKATLADKGA
                     ITFTAKDKAGNQAEPKTVTINVLPLKDSNEP"
BASE COUNT         4825 a         2862 c         3187 g         4608 t
ORIGIN      
        1 aaaacctgct ttttaatttg gaaaatgatc caattgaccc tcatttttca tctcttgtcc
       61 ttagctggac tgattgtgtt tggggtgggg ccagcctggc aaaccattgt caccctattt
      121 ttaaaaacac ctcaagaaga aaaccactat tctttgaaaa gggctgttca actttggaaa
      181 tcttatttca aagaagctaa tctaagattt ggtctttttg ccctgacttt ccttttttta
      241 acctttaatt tgcattgggc agtacagttt ccatccttgt tttggtttac cgtgtctttc
      301 ttgattgtaa tagccatggt ttggctaacc attacctatc tttacatggt tttttatgca
      361 gtgagctatg agattaacct ttggaataat atgaaattag cctttatcag cgtcttttta
      421 agtttcaaat cctttttact catgctgagt gttttgctgg gaattacctt aatgacttgg
      481 caatacaaag gcctttatct ctttttaacc ttcggagctc tagtcttttg cttggacttt
      541 gtcactaagg ctaatcgtcg actagtagat ggagtgattg atgagtgtta aaacattggg
      601 aaggtatgtg actaaccact ttaagcaggg ttttttgatt aatcgactga tgaaattata
      661 tagcctgcta attgtaagtt ttttcacttt agcagctatt ggcttgagca gttattctat
      721 tagtagtact tataaaaggg tggatgctga ggcgcagatg cgactggagg agactctagg
      781 tagattgcag ttacaaaacg atattaccct acgggtttta gaccagttgg ttggccgttc
      841 agaagattac cataacctct atcaatatat gacgttaacc ccaaaccaat atttttcaaa
      901 cgtttttgaa gactgggaaa aaggaaaaga cacggtccta ttttctgaag aggtgaggcg
      961 tctttttgac ttgtaccctg atgtgacttc tatgacccta ctgctagagg acagtagtgc
     1021 ttatctgtac gccgataggg tcattaagac gggtcgtttg ctttatagca agcctgaaaa
     1081 agtaaccgga aatgttttag ttcgctccat tcgaaatcca gaatcaggag atgtgacagg
     1141 tcgtctttac ctgacctttg acaaaccacc gactttggta gagacacagc aggatcacta
     1201 tttagctact tttgcttttg attactatgg ccgcaaactc ttccatcaag gaggtcggag
     1261 atttcctcat ttagaggctg aggttaaaaa ggctatcaag gcagatcggg cagctgatct
     1321 tagtaatctc agcaaggctt atcggatgca gtacaatcgc tcgggagacc tcttagctta
     1381 tgtggctgtc agaaaatcct atttattagc ggaagctgtc aggacagttt ttgtgtatgg
     1441 ccttgtttct ctactcttag cttggctgtt attgcagttg ctctttaggg ttttccgaaa
     1501 ttatatccag caggtttctg aaattacgga tactgttgaa atggttgcag caggcgattt
     1561 gtctttaacc atcgacaaca gccacatgga attggaactg tatcatatct cagaagccat
     1621 taatcagatg ttggccagca ttaaagctta tattgacgaa gtttatgtgt tagaggtaga
     1681 gcaacgagat gcccaaatga gagctctgca gtctcaaatc aaccctcatt ttttatacaa
     1741 cacgttagag tatattcgga tgtacgccct tagttgtcaa caagaagaat tagcagatgt
     1801 catttatgcc tttgcgagtt tgcttcgcaa caatattagc caagataaga tgaccacctt
     1861 aaaagaagaa ctggcttttt gtgaaaagta catttacctc taccaaatgc ggtatccaga
     1921 tagcttcgct tatcatgtaa aaattgatga gagtattgct gatttagcca ttcctaaatt
     1981 tgtcattcaa cctctcgttg aaaattattt tgtgcatggg attgattata gtcgccatga
     2041 caatgcacta agcatcaagg cattagatga gacggatcat ctcttgattc aggtgcttga
     2101 taatggacgt ggtattagtc aagagcgctt agcagatatg gaaaaaaggc ttcaagagca
     2161 ccaaacaaca ggcaatagct ctattggttt gcaaaatgtt tacctccgtc ttttccatca
     2221 ttttcgagac agggtttctt ggtccatggc taaggagcca aatggtggct ttatcattca
     2281 aattaggatt agaaaggatg tttaatgtat aaggtattat tagttgacga tgaatacatg
     2341 attctacaag gattaaccat gattattgat tggcaggctc ttggttttga cgtcgtttca
     2401 acagcaagat caggccagga agccttagcc tatttgactc agtacccagt ggatgtcatg
     2461 atttcagatg ttaccatgcc tggaatgaca ggactggatt taatagaagc agctaaaact
     2521 aatcatcctc agctacaaac cttgatttta tctggttatc aagaattctc ctatgtgcaa
     2581 aaggctatgg aattagaaac caaggggtat ttgctaaaac ctgtggataa ggcagaattg
     2641 caggctaaaa tgaaacaatt taaagactgg ttggatgccc agcaggctga atcaatccgg
     2701 caagaaacct atcatgacag ccttctgaca ctgtggttaa cagatgaact tagtgaaaaa
     2761 gaatttcatc aacttagcca ggatctcccc gagcatgccc taacaggatt taccgtgctg
     2821 tatgtggagt gttccgagtg gatagaagag atggaggctt attttaagca agaagaacaa
     2881 ccctgttacc tcaaaaaaga agaagacaag aagatttact tggcgatttt acttgggaga
     2941 gcccagcaag ccaaagcctt taccagtaat ctacaaaagc aattttcaga gaacatccaa
     3001 caaatcatcc taggagaaac ggttgatgac tgggaaaatg tttatgagag ctacaatcag
     3061 gtgcgccaga gtctttttta caatagccaa atgtcacaac caactgacaa aggcaacagt
     3121 aagcttgaga tgccagaatc tcggttgcaa ttttttgcct ttaacaaagc attgatgatt
     3181 ggtgatgagc caaccatctt ggccaaatta gaagccattt ttcatgaaat gaaggccttg
     3241 aacttctcac cagaggatgt gaagcatgtg tcgttcttgt tgttctctga catttaccgt
     3301 caatttccca ttttagataa aatgacttat ctgtccatgg taaaaactat tcataacagc
     3361 caatccattg atcgcatttt gagggaacta aaaaaagtgt tggatgtcac caatcagaac
     3421 aattcccctg aaaaacgtta ttcagattta gttagtgaaa ccattgattg cattcgcaag
     3481 gaataccatc aagaattaac ccttaaagct atcgctgata ggttacatgt caatggggtt
     3541 tatttagggc aatgctttaa aaatgaaacc gagcgtagtt ttacccagta cttaaatcat
     3601 gttaggattc aaaaagcgca acagttattg ctttacacca accaatccat caatgaaatt
     3661 gcttatgaaa caggttacaa taccaatcat tattttatca agatgtttaa aaagttgaat
     3721 ggtttatcgc caaaagagtt cagagatcgt tacaaggaca attaccaagc catcaagggc
     3781 gaccagtaac gtcaaacagg agattgatat gacaccagat agcaaggagc agacagtgac
     3841 tggctaccaa taccactata ttgatcaaga aggaagaaag cagcccttta atcaaggctg
     3901 gcgttttctt atggcagatg tggcgtgtgc tcaggaccct tcttttgatg attcgaactg
     3961 gcaggtgatt catttaccac atgatttcag tcttacgcag ccttacacaa gaaatggtga
     4021 agctgaaagt gcttataaat taggtggtgt cggttggtat cggcattatc tcgtgttaga
     4081 tgaggtttta gctggttgtc atgttgccat tacttttgag ggttcctata tggaaacgga
     4141 aatttatgtt aacgggcaat tcattggcaa acacttaaat ggctatcagg aatttactta
     4201 tgatatctct gatgttgtga cttttggagc tgaaaatctt ctggctgtcc gggttgaaaa
     4261 caaggttccc agttctcgtt ggtattcagg cagtggtctt tatcgtgaag tgagtttgtc
     4321 tgttcttccc cagcttcatt ttgtggcaga ccaggtagca atgaccttgg ctgatactgc
     4381 tgtgcaggag aaagggcaac agaaagttga tttacgcttt gccctcaatc agtctattca
     4441 gacttgccac taccaattga gcctctgttt atgggaacaa tcacattgct caaaagacaa
     4501 gaaactcctt tatcaagaaa cagaagtgcc tctagctgat ttggccttcc aaagacagta
     4561 tggtcttaca ctgtctttgg aagaactcca attgtggtca ccagataatc ctcacctcta
     4621 cgatttagaa ctcactcttt actatcaagg tcaggtcatt gattgctttt gccttgaaac
     4681 tggttttcgt cagctgactt ttatggccaa tcaggggctt tttgtgaatg gcagagctgt
     4741 caaattaaag ggagtttgtt tgcatcatga ccaaggaggc ctaggagctt gtgcttacga
     4801 agatgcccta gccagacaat tagtcctgtt gaaagacatg ggggctaata ccattcgtag
     4861 cacccataac ccaagtagtc ccaaacttag gcaactagcc aatcgtctcg gcttttttgt
     4921 gattgaagaa gcctttgaca cctggaccta tgccaaaaat ggaaatgtta atgacttttc
     4981 aaactatttc catcagacca tcggaactga aaatgcaagc tatcttcaaa gagtgaggtc
     5041 acctgaaacg agctgggccc agtatagtat tgaggccatg gtctggtctg ccaagaatga
     5101 cccgtcagtg ttgatgtggt cgattgggaa tgaattgatg gaaggttttt cagccgatgt
     5161 cagtcattac ccagagctaa caagacaaat gtgccaatgg attgctgcta ttgacacctc
     5221 acgtcccatt acctttgggg acaataaatt aaaagaagct gacttttgct ggcatgagga
     5281 agtgtcgcaa atggcaactc ttcttagtca attagaccat ccccagggac tcattgggtt
     5341 aaactatgct aatggaaaag actatgataa gcttcatgag gagcactctg attggctgtt
     5401 gtatggttct gaaacagcct cagccattac cagcagggct tattataaag agacaaaaaa
     5461 agttcttgac tccggttatc acctaacctc ttatgatcat gctaaggtcg attggggtgc
     5521 attcgctagc caggcttggt atgataccat tacccgtgat tttgttgctg gcgaatgcgt
     5581 ttggacaggc tttgattatt taggagaacc tacaccctgg aacaagatcg acagcggtgt
     5641 tgtaggcctt tggccttctc ctaaaaatgc ctactttggc attttagaca cggcaggctt
     5701 tccaaaagat agctattatt tttaccaaag tcagtgggcg caggggcaga ccaccttgca
     5761 cctgttacca gtctggcaga aggaccagct ttgtttcgat gagcagggat tggtggaggt
     5821 tgtggtttat agcaatgctg ccagtgttca actgatgttt gaagatgagc agggcaacgt
     5881 aacagattat ggaaggaagg catttcatac ttatagcacg ccaacgggac acacttatca
     5941 gctttaccaa ggagctgacg ctgctaagaa tcctcacgag aatctttacc tgacttggcg
     6001 agtgccctat caaaaagggt tgctaagggc agtggcctac gacatttctg gtaaaagcat
     6061 tccaaaaacc agtggacgtt ctcaggttag aacctatggt tcagtggcaa agctatcttg
     6121 gaaagccttt gaagcaccta ttgacgctcc ttgggaattg ctttatctgg atttatcact
     6181 cttggatagc cgaggtgaat tggtcagcca tgctcaagat tggctaaagg ttcaagtaga
     6241 aggaccagcc catttgttgg ctcttgataa tgggaatccg actgaccaca ccccttatca
     6301 ggagcctcta cgccaggcat atgggggaaa attattagcc attttagccc tcactggaga
     6361 ggcgggacac attaaggtca cggcaaaagt ctcgcagcaa cttagcagtg tttttcagac
     6421 acaagtagcc tgccgtgccc ccaaagaaca cttagtgaca ctacctttag agaaatatca
     6481 gtttaaaaag agccgactga caagtcaaca agaaattggt caatcttgtc taaagaccac
     6541 acaattcctt tctgttttgg agaacaaaga cacccagcaa aaagcaggta aggttagcac
     6601 acctcacatt cttatcacca gttaccagca ctatttatct ctgcccttgc aggtttgttc
     6661 tcaggcattt gattttaaaa aaggcgacga tatcttcttg cctcagtggc tccaagtcaa
     6721 agatgcccaa ggttcttttg tgaaaaaagc ttttccaatg acatggtacc taaaagagaa
     6781 aatctttgcc aagcctattc ttgtcacggg ctatgtttct tgttttgggc aatcaatacc
     6841 ggttgaggca aggcttaacc tagcacaagc tagcaaaaga cggcaccagg atctcagttt
     6901 agtcgccaag caatccctgg ccacaagtca aaaaaggcta acttattgct accaatatga
     6961 tacggtgcaa gccataggag ccattagctt gcaaaatcac acagacacat ctcttagtgt
     7021 ttctgtggca atcagttatg gtagtcagag ctctattaag caagaaacct tgatgctagc
     7081 tgatagccac ttgagccagt tagaagaggt tattttcttc gatgatattt tgactgtttt
     7141 gtatctagta gtgacccttg aagtcattga aggacctgac ctaactgaag acagtattca
     7201 aattggcctt tgggatttag catgaagcat aagtaaaaga agacttcttt ggcaccaggt
     7261 ctgataaatt ttgtaaaata aggtataata aaaaacaaat aggcgtcctt tattacgtga
     7321 aaggcgcctt tacaaggttg aaatggaaag tgagatttct aaatgtcaga acgtttatca
     7381 ggccacaccc tgctggtctc tttgcttgct actcctattc gtcatagttt gtctcccaaa
     7441 atgcacaatg aagcctatgc taaactgggg ctggattatg cctaccttgc ttttgaagta
     7501 gggacagagc aattagcaga tgccgtgcaa gggattcgtg ccttaggaat tcgtggctcc
     7561 aatgtgtcta tgccaaataa agaagctatt ttacccttat tggatgactt gtccccagct
     7621 gctgagttgg tcggtgcagt caatacggtt gtcaataagg atggtaaagg acatctcgtg
     7681 gggcatatta cagacggcat aggagcgctc agagccttgg ctgacgaagg agtgtcggta
     7741 aagaacaaaa tcattaccct agcaggagtt ggtggggctg ggaaagctat cgctgtccag
     7801 ctcgcttttg acggcgccaa agagattcgt ttgtttaacc gacaagctac acgtctaagt
     7861 agcgttcaaa agcttgtgac aaaactgaac cagctaacaa ggactaaagt aacccttcaa
     7921 gacttggaag atcagactgc ctttaaggag gccatcaggg aatctcatct atttattgat
     7981 gctacaagtg taggaatgaa gcctttagaa aatctaagtt tgattaccga ccccgagctc
     8041 attcgcccag acttggtggt ctttgacatc gtgtacagcc cagcagaaac caagttacta
     8101 gcctttgccc gtcaacatgg cgcccaaaaa gtcattaatg ggctagggat ggtgctttat
     8161 caaggagcag aagcttttaa attaatcact ggtcaagaca tgccagttga cgctattaaa
     8221 cctcttttag gtgatgagta actcaacctt ttttaaagaa ctttttaagt aagcttaatt
     8281 atgatatatt aataagagat ggagcagtca tcatgggctg ctaaaattct ttttgcgaga
     8341 gctgttttta agagtaaaaa gcttatttga agagccttat ccctaacgtg atacaagtca
     8401 tagttccttt gaggtctgcc agataaccta ccgttttttt agaaaagaag ctcttataaa
     8461 aggaaaagtt caatgaataa aggagaatag caatggtaac aaaggaagtt gggcatgact
     8521 ttttagcacg tctaggaaaa aaacgcttaa gacctggcgg tgttgaggcg acaaactggt
     8581 tgattgaaca aggacaattc tctcaggatt gtcacgtttt agaggttgcc tgcaatcgct
     8641 gcacgacagc aatagagtta gccaaaacct tcggttgtca gattgatgcc gttgacttaa
     8701 accccaaggt tgtcaaagaa gcacaagaaa gggttactgt tgaggggtta acccgtcaca
     8761 ttacagtatc ccaagccaat gctttatcac taccatttcc agataaccat tttgatattg
     8821 tgattaatga agccatgtta accatgttga acgccaaagc caaggagaag gcactcaatg
     8881 aatattggcg tgtgttaaaa ccaggcgggc gacttttgac acatgacgta gcttatgaag
     8941 atccaaaaac aagacaagta cttgacgagt tacgtcaaac gattcatgtc aatgttgctc
     9001 ccttgtcact aacaggttgg caagccttat atcaagaaca cggtttttct caagtgactt
     9061 atcaccatgg gaaaatgtct ttgatgacac ctcgtggaat gattaaggat gaaggtctta
     9121 tgaacaccct gaacattatg agacgaggcc ttaagaaaga gaacagacaa cagtttttaa
     9181 agatgcgtcg tttttttacc agaacaggca aaaatctctg ctatattgct gtaaaaagtg
     9241 taaaataaac cttagaccaa cctgtccata caactaaaca gaccatagag gagttcgaag
     9301 atgtcttata tcaataatat tgagcacgcg aaggttcttg atttgacgca agaagtaatg
     9361 attgagcaag atcaaatgtt aagtagaaca ctagtccaaa ggcaagacct aggaataaca
     9421 gttttttcct tagataaggg ccaagaaatt gggcgacact catcaccagg agatgccatg
     9481 gttactatct taagtggctt agcagaaatc accattgatc aagagaccta ccgggttgct
     9541 gaaggtcaaa ccattgtgat gccagctgct attcctcatg ccttatatgc agtagaagcc
     9601 tttcaaatgc ttttagtagt agtaaaaccg gaggcttaac atgattgaaa atctctctca
     9661 actggtgcaa tatcatgatc atcaggtggc tagccgatcc ttgagccgtc tactgggatt
     9721 atctcaatct attgttttat atgccatggc ccaaggagaa agcattagtc aagagactag
     9781 tccaagagat aaagtaatac ttgttttaga aggtcagctc attttcgatt tagaggatca
     9841 aaaacaggtc ctaactcaag aaagtctcat agccatacct gcccaaaaag cccatcattt
     9901 agaagctaaa accgattgta aactattaca agtgctttta gattaagggc ttattgtcaa
     9961 ctgtattggg tggcctatgg aaaagcaaaa gagaagatga cgctcatctt ctctttttac
    10021 gtattcaaag cgatgacaat acgaatttta aagcttggcc ttctcatctc cagattaagc
    10081 cctttagctt ggtttatttt agaattcact aaaaaacctt aatgagtttc ctatctcctt
    10141 ataggtttct cattaaggct ttttagacga aataattgaa cgacaggcaa aagaaagatc
    10201 tttttacgag tgataccgtt ctcctatagt tccataaatc ctctttttga tgttgctgtt
    10261 agttattctt agctaaaagt tatattgtta tcaagacttg tcttgtagta acatgtttta
    10321 ggagttccag gataccgtta ttctcttagc aagcttaact agctagagtg gagtcactat
    10381 ttataataaa ctgtcgagct tctcgacaaa ttggtgatcg tctttgttaa tggctagggt
    10441 ggtaaaaccg gtttctgtgg gtaatataaa gataatatgt tgctcagaat tcttcttatc
    10501 tgttttaaag acgtcaaaga gggatgtcgc agagacatca gctctttcaa gtgttgttgg
    10561 taactggtaa cgttcaaaaa cttgaaggag gctatccaag tcctcttttg gcaagagtcc
    10621 tttggaaaca gccagtttag catcaaccac cataccgata gcaatggctt caccgtgatg
    10681 gtaaaaatca tggtgacaga gcgattcaat cgcgtggccc aaggtatgcc caaaatttaa
    10741 agacatgcgc aaaccatttt caaattcatc ttgttcaaca atccgttttt tattggaaat
    10801 ggattggtaa atcagacgtt caagtccctg cttatcggtt tctttttgaa aatcacgtaa
    10861 ctgctgaaaa agcggtctat catgaatgaa gccgattttg agcatctcac tgatgccgca
    10921 ggagaattcc cgttgcggta aggtctccaa aaaggtggtt gaaataatga tagcttctgg
    10981 agggtaaatg ctgccaatca tattggttag acctttaaaa tggactccaa ctttgccccc
    11041 gatactgcta tccacctgac taagtaaagt cgttgggatt tgaatgtagg gaataccacg
    11101 gtaataggtt gcagcgacaa aaccacccag atccccaaca acaccaccac caatagtaac
    11161 aatcatatct tttttggaaa aattttcagc gatgagctgg tcgtagatag cactgacacg
    11221 ttccaaggat ttggattgac ctcccggagg gcagacatgg acaaaagcgt tgtattgctg
    11281 agcaaattct tcaaagagag tctgatacaa gtggtagacc gtttgatcgg tgataaacaa
    11341 gagtttacgc tgcttgcgct caccaaggca atctgctaag gtttttaaaa catggtcggt
    11401 aaacaggata tcataatcct tgacccgaga atggacatga agtgtttgtg gcataagcac
    11461 tcctttcatc agtactaggt aacagagata acgcagtgat atttagagct taatcttgcc
    11521 aaagttcctg ccccaactgt tctaattgct tataatcaat ttgctgggca gcatctgaca
    11581 gcgcatggtc gggatcagga tgaacctcca tcatgatgcc attggcaccg acagccttcg
    11641 ctatcttagc agcaggtaag agtaaatctc gtctgcctgt cgaatgagac acgtccacaa
    11701 taatcggtaa atctgtcttt tgttgaataa tagggactgc cataatatca agcatatttc
    11761 tagtctccac atcgtagcca cgaacgccac gttcgcataa gataatgtta gattttccag
    11821 tgtcttgtaa gtaggaaagg gcgcccaaat attcctcgat agtagccatc aagcctcgtt
    11881 taaacaaaat aggcttatcg atgtgtgaca aggtttttaa aaattcaaaa ttttgcatgt
    11941 tgcgggcacc aacctgaatg acatcgagat agtcataggc ttcttctaat tggcgctcgg
    12001 acatgatttc actgaccgat agtaagccaa attcctgaca aacctcatgc aagtaacgaa
    12061 ttccttgcag gccaaggcct tgaaaagaag cagcagatgt tcttggttta taggcgcctc
    12121 ctctaaaata gttgtaacca agttttttgg cacttgaagc tgctaatcta atgtgatcat
    12181 aagattcaat ggaacaaggt ccaacaataa aattattctt agaacaagtc ttattttgaa
    12241 agtcagacat aatgtcaatc attcgtttga ttcctttact aattctgtat ggcgtgctct
    12301 atgggataac gtcttattta ggaatacaaa atctcgttgt tatccatgaa gcacccttag
    12361 cctgtgaaat gataccataa acaagatgaa atagcaagta aaccctagat tgtctatgtt
    12421 cttagtcatt tttattaaaa aaggcttgct agctataaaa aaatactgcc ccaaatctta
    12481 ttaggacagt attttttata gatgccacca ccgggaataa ttagaaattt tcttatagtt
    12541 tttaaacata aaataaccaa taatatcaag ggatttatca ttttttattg taaaaactaa
    12601 tagagtgatt tctattttta agaacaattt tagaacaaaa attgacttat tcagttagta
    12661 tattttgtaa agtaattaat aagttttgat tgttatataa agtatatttg ctataatggt
    12721 aacttctaaa atagtctatt atgaagggaa tattaagcaa aatgaatatt tttcgattac
    12781 tatattgtct taagcttttg cttatattct agtgggctaa ttcccactat ttttttaaat
    12841 gtccttgaaa aattagaaga agtggaaaat ccaagtaaat tggaaacttc ttctactttc
    12901 ttattttcgt ctagtaactg cttagcagct tcgattttta agtttataaa atattgttga
    12961 atagtgatgt gtttttcttg cttaaatagt tttcgtaact tcgattcact catgtgacat
    13021 tctttagcta tttcttctgt tttaataaag cgatttagat tattttcaag atatcgaatc
    13081 actgcgacaa tagtttgaga atgaggggtt tcgagatgtc tttttaaaga atcaacttgt
    13141 tgagtataga ataagatagc agtatcacgt aattttaaaa cttcatttag acttatagtt
    13201 gactcggttt cttttataaa acgatctcgt gctcgataag ctgtttcgat atcaagtcct
    13261 gcttggatgg tagcctgtga gagacgatca taaataatga tggagtagtt tttttcagat
    13321 cgcaattcat ctccactgat tataggtacg atagaattgc taaagtgttc cactgtttct
    13381 ttcaaaagaa attcactacc tgatttaaca gcttttagaa tattattttc aaaatgatac
    13441 tgatatttgt aagaactcat attctgttta gaaaattgct taattttatc tagatcaaag
    13501 gagttgctcc accctttcgt gtagtaatgt aagggctctg ataatttatc tttcacaacc
    13561 ccagttagac aataattggt tactattagt aattctcgga tttgactaag tgagaagaaa
    13621 ggtaattggg atagtctatc aataaagtag tcttgttcac tagcattgat ttgtgtttca
    13681 gtcagctttt tcttaagaag taaaggatca atagcattgc tacgccaggg accgatgata
    13741 atataatatt gttgaatatg gtggacaaga aaaagttctc ctaaagagcc ataatgaaat
    13801 aaaaaatcat gttgagtttt actaaagtta cttaaaataa ggtaatgatt gtaaaaaagt
    13861 gaaatagttt tatcggattt atactctctg ataacagaaa aattttgatc acaaacagta
    13921 atagttagac ctagaagact gtgtaatgat ttcaacaata aagaatcaat catatgagtt
    13981 accacctttg atttaatatg ttatctattt tagagaagat ttctttaaag tcaacagaat
    14041 aattaggtag tcggtatttt aaccaaatat gatttaaaaa atcgaaaaag tcaaattata
    14101 tataaaaaaa aaagcagatt agattagata aaaaagtata gatattctaa tatttgttgt
    14161 ttaagcctat aatttaatct gtatagagtt atacagagta aaggagaata ttatgtttag
    14221 aaggtctaaa aataacagtt atgatacttc acagacgaaa caacggtttt caattaagaa
    14281 gttcaagttt ggtgcagctt ctgtactaat tggtcttagt tttttgggtg gggttacaca
    14341 aggtaatctt aatatttttg aagagtcaat agttgctgca tctacaattc cagggagtgc
    14401 agcgacctta aatacaagca tcactaaaaa tatacaaaac ggaaatgctt acatagattt
    14461 atatgatgta aagaatggat tgattgatcc tcaaaacctc attgtattaa atccatcaag
    14521 ctattcagca aattattata tcaaacaagg tgctaaatat tatagtaatc cgagtgaaat
    14581 tacaacaact ggttcagcaa ctattacttt taatatactt gatgaaactg gaaatccaca
    14641 taaaaaagct gatggacaaa ttgatatagt tagtgtgaat ttaactatat atgattctac
    14701 agctttaaga aataggatag atgaagtaat aaataatgca aatgatccta agtggagtga
    14761 tgggagtcgt gatgaagtct taactggatt agaaaaaata aaaaaagata ttgataataa
    14821 tccaaaaaca caaatagata ttgataataa aattaatgaa gtcaatgaaa tagagaaatt
    14881 gttagttgta tcgctaccag ataaaattaa gtattcgcca gaggctaagc ataggactgt
    14941 tgaacaacac gcggaattag atgcaaaaga tagcattgca aatacagatg aattgccatc
    15001 aaattcaacg tataactgga aaaatggtca taaaccagac acctcaacat caggtgaaaa
    15061 agacggaatt gttgaagttc actatccaga tggtactgtt gatgatgtga atgttaaagt
    15121 aaccgttaca tcgaaaaaaa ctgataatac agctccaaca ttaaccgtca ctccagagca
    15181 acagactgtt aaagtggatg aagatattac ctttacggtt acagttgaag acgaaaatga
    15241 agttgaacta ggtttagatg atcttaaagc taagtatgaa aatgatatca ttggagctcg
    15301 tgttaaaatt aagtatctta ctaaagaacc taataagaaa gtcatggaag tgacaattat
    15361 gaaagctact ttagcagata agggcgcaat tacctttact gcaaaagata aagcaggtaa
    15421 tcaagcagaa cctaagacag ttaccatcaa tgttcttccg cttaaggata gcaacgaacc
    15481 aa
//
LOCUS       DAUVUY010000039        15430 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.039, whole genome shotgun sequence.
ACCESSION   DAUVUY010000039 DAUVUY010000000
VERSION     DAUVUY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15430
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.039"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..486
                     /locus_tag="VOX58_001066"
     CDS             <1..486
                     /locus_tag="VOX58_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5878298.1"
                     /translation="ALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKPFSL
                     KELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFLMLN
                     QGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYCLLE
                     K"
     gene            495..1727
                     /locus_tag="VOX58_001067"
     CDS             495..1727
                     /locus_tag="VOX58_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HES5878299.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPGTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSVMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
     gene            2020..2898
                     /gene="mvk"
                     /locus_tag="VOX58_001068"
     CDS             2020..2898
                     /gene="mvk"
                     /locus_tag="VOX58_001068"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HES5878300.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLVIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            2880..3824
                     /gene="mvaD"
                     /locus_tag="VOX58_001069"
     CDS             2880..3824
                     /gene="mvaD"
                     /locus_tag="VOX58_001069"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HES5878301.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGVLQNDEEHTKISTIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFDTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDEWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            3817..4830
                     /locus_tag="VOX58_001070"
     CDS             3817..4830
                     /locus_tag="VOX58_001070"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HES5878302.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFSHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHSAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQNARDTLISKWQEADIALLYQERWEEN
                     DQP"
     gene            4817..5806
                     /locus_tag="VOX58_001071"
     CDS             4817..5806
                     /locus_tag="VOX58_001071"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HES5878303.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(6036..7313)
                     /locus_tag="VOX58_001072"
     CDS             complement(6036..7313)
                     /locus_tag="VOX58_001072"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HES5878304.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDHDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQTGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(7300..8475)
                     /locus_tag="VOX58_001073"
     CDS             complement(7300..8475)
                     /locus_tag="VOX58_001073"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HES5878305.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRSKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHRRIYHTNDKN"
     gene            8684..9523
                     /locus_tag="VOX58_001074"
     CDS             8684..9523
                     /locus_tag="VOX58_001074"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HES5878306.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
     gene            9603..10100
                     /locus_tag="VOX58_001075"
     CDS             9603..10100
                     /locus_tag="VOX58_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HES5878307.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAVL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            10420..11649
                     /gene="clpX"
                     /locus_tag="VOX58_001076"
     CDS             10420..11649
                     /gene="clpX"
                     /locus_tag="VOX58_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HES5878308.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIQILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            11659..12258
                     /locus_tag="VOX58_001077"
     CDS             11659..12258
                     /locus_tag="VOX58_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HES5878309.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPKDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTSRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDNSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            12406..13155
                     /locus_tag="VOX58_001078"
     CDS             12406..13155
                     /locus_tag="VOX58_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878310.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKKSVKCIT"
     gene            complement(13208..15307)
                     /locus_tag="VOX58_001079"
     CDS             complement(13208..15307)
                     /locus_tag="VOX58_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HES5878311.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATTSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKERQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGFNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILEEGQLVIRQKDQ"
BASE COUNT         4819 a         2720 c         3108 g         4783 t
ORIGIN      
        1 gctttaaagg agattcgcgc ttcaggaaat tgctcacata ttattatgct gacggctatg
       61 gcagaaatta atgaccgtgt tactggacta gatgcgggag ctgatgatta tctgactaaa
      121 ccgttctcat taaaagaact tttagctaga ttaaggtcga tggaaagacg agtagaaagt
      181 tttactcctc aggtattaca atttgctggt gtcacattaa atattaatga gcaagaattg
      241 agtgccggta atgctatacg attagcttct aaagaaggta aattaatggc ctttttaatg
      301 ttaaatcaag gaaagtactt ggatactaaa acactttacc aacatgtttg gtcagaccaa
      361 gaagattatg atacttctat tgtttgggtt tatatttctt atcttcgtca aaaattatta
      421 gctattcaag caaatgtcat tattgcaggt gataaagata ctagctattg tttattagaa
      481 aaataggagg tgacatgttt aatcgtatcc gtattcgttt tatcatgatt gcttctattg
      541 ccatttttat tattttaagt tcaattgttg gtattattaa cacggcacgc tgttaccaaa
      601 gtcaacaaga aattaaccgt attttacatt taatttcctc aaataaaggt aaattacctg
      661 gcactacgga aagctctaaa cgacttggaa cgaaactgtc tgaagatagc ctaagccagt
      721 ttcggtatta tagtgtcatt tttaatgcta atggacatct actttcttct aatactgcta
      781 atatttcagc tttagataga gaagaagccc aatattttgc tagacttttt gctaagtctg
      841 gggaagaaaa aggcagttac cgtcaccaag atagtgttta ttcgtacttg attactcagc
      901 tgccaaatga agaaaaactg gttgtaattt tagatactac cttttatttt cgtagtgtag
      961 gagatttatt agctgtttca gtgatgttgg cctttggagg atttatcttt tttgttgttt
     1021 tagtgagtct tttttccggt atggtcatca aaccttttgt ccaaaattat gagaagcagc
     1081 gtcgctttat tactaatgca ggccacgagt taaaaacacc actagccatt atctcagcca
     1141 ataatgaatt ggtcgaatta atgactggag aatcagaatg gaccaagagt acaagtgatc
     1201 aggtgaagcg attgacaggc ttgattaatc aaatgattac tttggctcgt ttggaggaac
     1261 agccagatgt ggtattacat atggtagact tttcagctat tgctcaagat gcagctgaag
     1321 attttaagag tctggttttg aaagatggta aacgttttga tttgacgatt cagccgaata
     1381 ttatgattaa agcggaggaa aagtcattat ttgaacttgt gacaatttta gttgataatg
     1441 ccaataaata ttgtgatcct aaaggcttgg tcaaggtatc tttaaccact attggaagaa
     1501 gacgaaaacg agcgaaatta gaagtttcta atacctattt ggaaggaaaa tccattgatt
     1561 acagccgctt ttttgaacgc ttttatcgtg aagacgaatc ccataatagc aaagaaaaag
     1621 gctatggaat tggtttatct atggcagaaa gtatggttaa gttatttaaa ggaacgataa
     1681 ctgtaaatta taaaaacgat gctattgttt ttacagtggt gatttaactt aaacattttt
     1741 cagtaccttt agctagtaat aatgtctagg caaaggtaat aaatgatgct taaccagccc
     1801 atgaggctgg ttttaacgtt taatttagta gaagagtgat taaagaaggt agtttgaaat
     1861 aagaaaattt ttgttatgat cctaaaaaga gtagatagtc tttattgtta atttcagtga
     1921 agaaatgatt ttaaaaagat atatcatcgc attttctaat catttagtgt aaaatagtaa
     1981 ggtaaaagtt tagaagaatt agtaatgaaa agataaaaaa tgaatgaaaa cattggatat
     2041 ggtaaggcac acagtaagat cattttgata ggtgagcatg ctgttgtgta tggctaccca
     2101 gctattgctt tgcctttaac agatattgag gtggtttgtc atatttttcc agctgataag
     2161 ccattggtct ttgattttta tgatactcta tcaacagcca tttatgctgc cttggattat
     2221 ttgcagcgac tacaagaacc aattgcttat gagattgttt cacaagtgcc acaaaagcgt
     2281 ggtatggggt cttcggcagc cgtttctatt gcagctatta gagccgtttt ttcttattgt
     2341 caagaacccc tctcggatga tttgttggaa attttagtca acaaggcaga aattattgcc
     2401 cacaccaatc ctagtggttt agatgccaag acatgcctta gtgaccatgc cattaagttt
     2461 atccgaaaca ttggctttga aactatcgaa atcgccttaa atggttatct tgtcattgca
     2521 gatacaggga ttcacggtca tacacgtgag gccgtcaata aggtagcaca gtttgaggaa
     2581 acgaatttgc cctatctggc taaactagga gctttgacac aagcccttga aagagcgatt
     2641 aaccaaaaaa ataaggtggc tatcggtcag ctcatgacac aagcgcactc tgctttaaag
     2701 gccattggcg ttagcatcag taaagctgac caacttgtgg aggctgccct aagagcaggt
     2761 gctttaggcg ctaagatgac aggcggtggc ttgggtggct gtatgattgc cctagcagat
     2821 accaaagaca tggccgaaaa aatcagtcac agattaaaag aagaaggagc cgtaaacacg
     2881 tggatccaaa tgttataacc gtaacatcct atgctaacat tgctattatc aaatattggg
     2941 gaaaagagaa tcaagctaag atgattcctt cgacctctag catttctttg actttggaaa
     3001 acatgttcac cacaaccagc gtttccttct taccagatac tgcaaccagt gatcagtttt
     3061 acattaacgg tgtcttgcaa aatgacgaag aacataccaa aatttctact atcattgacc
     3121 aatttcgcca acctggtcag gcttttgtaa agatggaaac tcaaaataat atgccaacag
     3181 ctgcaggttt atcatcaagt tccagtggct tatcagcctt ggttaaagcc tgtgatcaat
     3241 tatttgacac tcagctagat cagaaagctt tagctcaaaa ggctaagttc gcctcaggat
     3301 catcttctcg ttcttttttt ggcccagttg ctgcttggga caaagatagt ggtgctattt
     3361 acaaggttga gactgacttg aaaatggcca tgattatgct ggtcttaaat gctgcgaaaa
     3421 agccaatttc tagccgagag ggcatgaagt tatgccgcga tacctcaact acatttgatg
     3481 aatgggtaga acaatcggca atcgattacc aacatatgct tacctatctc aaaactaata
     3541 attttgagaa agttggtcag ttaacagaag ctaatgcctt ggccatgcat gccacgacaa
     3601 agacagccaa tcctcccttt tcttacctaa caaaagagtc ttaccaggct atggaggctg
     3661 tgaaagaact gcgtcaagaa ggttttgctt gttattttac catggacgca ggtccaaatg
     3721 tgaaggtcct atgtttagaa aaagacttgg ctcaactagc agaacgactt ggcaagaact
     3781 accgtattat cgtttcaaaa acaaaggatt taccagatgt ctaattattg tgtgcaaaca
     3841 ggtgggaaac tatacctcac aggcgaatat gctatcttaa caccaggaca aaaagcctta
     3901 attcacttta ttccactgat gatgacagca gaaattagcc cagcagccca tattcaatta
     3961 gcttcagata tgttttccca taaagcgggc atgacacccg atgcctctta tgcactgatt
     4021 caagcaacgg ttaaaacctt tgctgattat ctaggacagt caattgacca actggagcca
     4081 ttttccctaa ttataacggg aaaaatggag cgcgatggca aaaaatttgg cattggttca
     4141 agtggtagtg tcaccctctt aaccttaaag gccttatcgg cctattatca gatcacttta
     4201 accccagagt tactctttaa actagcggct tataccttgc ttaagcaagg agataatggg
     4261 tctatggggg atattgcctg tatcgcttac cagactttag tcgcctatac ctcctttgac
     4321 cgagaacagg tcagtaactg gctgcagacc atgcccttaa aaaaacttct cgtcaaagat
     4381 tggggttacc atatccaagt cattcaacca gccctgcctt gtgacttttt ggttggctgg
     4441 actaaaatac ctgctatttc aaggcagatg attcaacaag tgacagcgag cattacccca
     4501 gctttcttaa gaacaagtta ccagctaacg caatcagcta tggtagcttt gcaagaaggt
     4561 cataaggaag aactcaagaa aagtttagca ggagcaagtc atctcctaaa agagcttcat
     4621 tcagctatct accatcctaa gctagtaacc ttggtagctg cttgtcagaa gcaagatgct
     4681 gttgctaaat cttcaggttc tggtggtgga gattgtggca tcgcgcttgc ctttaatcag
     4741 aatgccagag atacccttat ttccaaatgg caagaagctg atatcgcatt actttatcaa
     4801 gaaaggtggg aagagaatga ccaaccgtaa agatgatcac atcaaatatg ccctcaagta
     4861 ccaatcgcct tataatgctt ttgatgacat agaactcata catcattcct tacctagcta
     4921 tgatttgtct gatattgatc tcagtactca ttttgccggg caagacttcg actttccctt
     4981 ttacatcaat gccatgacag gaggaagtca aaaaggcaaa gctgtcaatg aaaaattggc
     5041 caaagtagca gcagcaacag ggattgtcat ggtgacaggg tcttatagcg ctgctttaaa
     5101 aaatcctaac gacgattcct atcgtttaca tgaggtggca gataacttga aactagccac
     5161 gaatattggt ctagataaac ctgtggcgct aggacaacaa acggttcaag aaatgcagcc
     5221 cctcttttta caggttcatg tgaatgtgat gcaagagttg ctgatgccag agggtgagcg
     5281 cgtctttcat acctggaaaa aacacctcgc tgaatacgct agtcaaatac cagttcctgt
     5341 cattctcaaa gaagttggtt ttggcatgga tgtcaatagt atcaagctag cacatgacct
     5401 aggcattcaa acctttgata tttcaggaag aggagggacc tcatttgcct atattgaaaa
     5461 tcaaagagga ggagaccgct cttacttaaa cgattgggga caaaccaccg ttcagtgctt
     5521 gctaaatgca caaggactga tggaccaagt ggaaatctta gcttcgggtg gtgtcagaca
     5581 ccccttggac atgattaaat gtttcgtcct aggagcacgt gcagtgggac tctcacgcac
     5641 cgttttagaa ttggtcgaaa aatacccaac cgagcgtgtg attgctatcg ttaacggctg
     5701 gaaagaagaa ttaaaaatca ttatgtgtgc tcttgactgt aaaactatta aagaattaaa
     5761 gggagtcgac tacttactat atggacgctt gcagcaggtt aattaggctt catcacctct
     5821 acaatagtgc cacctactac aaagatgata gagctttttt agaacataat ataaaatatt
     5881 tataaatacc tcatcaaact cagaatagag tagatgagta aatagagtat gttatggaga
     5941 ctatttctaa gacttgtatg tatagaaaca aagattaaac acccatgtaa accaattgtt
     6001 tacatgggtg tttgccttat attagttgtt taaatctaag actgtcttag ctgggataag
     6061 taaaaatgtg ctttttctag atttattggt ttatcagcta gtaaagcatt aactaaagga
     6121 gcaatttcgt cttgggttgc ccccgccaaa agagctaatg atttcgcatg tagtttcatg
     6181 tgaccagttt ggatgccaga tgaggtcaga gcttttagtg cagcaaagtt ttgagctaac
     6241 cccacagatg caatcaattg ggctaatgtt ttggcatcag gttgattaag aagatcatgt
     6301 gcgatagaaa cagtagggtt aagcccgata gatcccccct tactagcaat aggcataggg
     6361 agggtcattt ggccaagcag aacttgttta tcttggtcaa aatgccactg actaagtcct
     6421 tgatagctac catttcttga ggcataagca tgggcccctg cttcaatagc acgccagtca
     6481 tttcctgtgg ctattaccac tgcatcaatg ccattaaaaa tacctttatt atgagtagta
     6541 gcccggtaag gatctacttg agctagtcgg ctggctagct ctattttttg agctaactgt
     6601 agggattttg ttttatcgtg atctaaaagg cgcacgggaa tctcacaact agtagtaaca
     6661 agcgactctg ttgcgtaatt agataaaatc gccattagac aatgcccctt agacagtctt
     6721 gttaaatcag gaacaagagc ttccatcata gtattgacca tatttgctcc catagcttct
     6781 tgggtatcaa cagtcagata gaaaatcaaa aactctcctt tttcttccaa atgaatggtt
     6841 ctagcgcctc cacctctttt aacaatggaa ggataagctt tatttgccaa tgcaattagc
     6901 ttttttgttt tatgaaggat ggcggcttta gcgttatcta tttggtcgat atcgtaaaga
     6961 acaatttgac caatcatttg tcggtttagg gtttgagctt taaaaccacc tgagcgtttg
     7021 attagttttg ctgcgaaaga tgctgcagca acaacagaag gttcttccgt gacaaaaggc
     7081 atctgataag ttgaaccgtt gacaagaaaa tcaggagcta tgctaaaggg caaagccaat
     7141 cgtcctaaga cattttcagt catttgatta gctgtttgga taggcaaaag aacgtctgtt
     7201 ttgagttgat ttaagttttc agcattaagt agtttaaatt tttcgataag ttggaggcgt
     7261 tcttcgaatg ttttctttga aaaaccgctc cagttaagat tagtttttgt cattggtatg
     7321 atagatcctt cgatgctcct tgatggctgt tagagcaaat tttccagtta agtaaatgtc
     7381 aaaattggca ttaccattat catctaattg gacttgttcg tagaaaaggt cttcgtattt
     7441 agaaacggaa agacgtgttc gttgatccag taaagcttgt cgattagtca ttaacatttt
     7501 atcgtaccca gcaactaact gaccggaata aaattcactt acggcaccgg aaccatagct
     7561 aaaaaaacca attttatctc cagattgtaa tactttacta ttttcaagta aagataacaa
     7621 tcctagataa agtgagccag tataaatatt tccaatttgt ttgctataag taatagaagc
     7681 ttgaaaggct tctattagtt tttccctgtg ttcaggaggt actgtgttat ccataatgtt
     7741 atttagacct tttagggcta acttgggaaa tggaatatgg aaacaaactg cagcaaaatc
     7801 agataactga aggttttctc ttttctgata agcttgccac gtggtttcta agcaattgag
     7861 atattgcttg gtagagtaaa taccatcaac ataaggtgtg aaactatagt taggcctcca
     7921 aaagtccata atatccctag tttgagccac attatcttca tttagagcaa gaatacgagg
     7981 gttagcagtt accaaaagag caatactacc agagccttga gttgattcgc caggagatcc
     8041 tacaccgtat ctagcaatat cactagcaat aacaaggaca cgagaatctg gcttagaagc
     8101 cacatgcagt ttagcatagt ctagagcagc agttgcgcta tagcaggctt gttttacttc
     8161 aaaggaacgt gcaaaaggct ggataccaac taagtgatgt acatagatag ctgatgcctt
     8221 tgactgatca gtactcgatt cagttgccaa aataaccata tcaatcttag atcgatcttc
     8281 gtcggttaag atttgatctg ctgcagaagc agctaaagta ataatgtctt cagtgattgg
     8341 tgccacacta aaagactcaa tgagtagccc ttgactaaat tttgctggat ccacttggcg
     8401 tgcaagcgct aaatcttcca atttcaagac atattgactg gttgcaaaac caatcttatc
     8461 aattccaatt gtcatatttg tccttctttt agttattttt atagtcttac ccattctatc
     8521 acattttact gcaaaattag actgataaag ttatttgttt tatcctttaa tagttaaaat
     8581 gttcatgacc acatgaagtt tttcaaatgt ttatttattc tggtattttt tggtaaaatt
     8641 agttagaata gtatgaagta tgcttacaga aaggttatta gagatgacaa aagctgacca
     8701 gatttttaaa gctaatattc aaaaaattat aaacgaagga tcattgagtg agcaagcacg
     8761 tcctaaatat aaagatggtc gaacagctca ttctaagtat attactgggg cgtttgctga
     8821 atatgattta gctaaaggag agtttcccat cacaacttta cgacccattc caattaaatc
     8881 tgctataaag gaattatttt ggatatatca agatcagtcc aacagtttag atgtgctaga
     8941 agctaaatat aatgttcatt attggaatga atgggaagtt gaccagacac gtactattgg
     9001 gcaacgttat ggtgctgtag tcaaaaaaca tgatatcatc tcaaaaatac taaagcaatt
     9061 agcagaaaat ccttggaatc gtcgtaatgt catttctctc tgggactatg aagcatttga
     9121 ggagacaaaa gggttgttgc catgtgcctt tcaaatcatg tttgatgtta gacgtgtagg
     9181 ggaagatctt tatttagatg ctagtttgac gcaacgttct aatgacatat tagttgctca
     9241 tcacatcaat gccatgcaat acgttgcttt gcagatgatg attgctaaac attttggatg
     9301 gaaaataggt aagttctttt attttgttaa caatttacat atttatgaca accaatttga
     9361 tcaggctcaa gaattactaa agcgacagcc agttgctagt cagcctaagc ttgttttaaa
     9421 tgttccagat gggactaatt tctttgatat taaaccagat gattttgaat tacaaaatta
     9481 tgatccagtg aagcctcagt tgcactttga ccttgctatt taaactttat ttagattttt
     9541 tgtgagcatt ttgttcataa atttggtaaa ataaatagag tttaaaatga aggaaataat
     9601 taatgacaaa ggaaattatt gctatttggg cagaggatga agcagggctt attggtgttg
     9661 cgggtaagtt accatggtat ttaccaaaag agttagaaca ttttaaaaag acaacgctac
     9721 atcaagcagt cttaatgggc cgtgtgactt ttgaaggaat gaattgcaaa agacttcctc
     9781 aacgtcaaac ccttgtcatg acaagaaata gagattatca agtagatgag gtgttgacaa
     9841 tgactagtat tgaaaaagtc ttggagtggt accatgcgca ggataaaaca ctttatatca
     9901 ttggtgggaa taaggtttta gaagcgttta atggttattt cgatcgtatc ataaaaacag
     9961 ttatccatca tcgttttaaa ggagatacct accgaccaaa tctagatttt agtcatttca
    10021 cgcaagaaag ccaaacgttc tatgctcgag acgctaaaaa tccttatgat tttaccgtta
    10081 ctgctttaaa acacaaatag tctgaggaga tgataagcga tgcaacgtag tatttttgga
    10141 gtatttacgg cttttttgtg cgtgatttgt gttctttgtg ccatcccagc ttttaaaaag
    10201 aatcgttatg gcttaggaag tctgttctta ttaaatgcgt ttactaatct ggttaatagt
    10261 attcacgcct tctatggtac attgttttga gatgtgactt gcgaagacag cactggtgaa
    10321 gatttagcta tcaagcaaga atcactaaaa accaatacaa cacattgact aatattaaaa
    10381 ttcagtaaaa gataagagaa agtaagagaa ttataagaaa tggcaggaag tagaactaac
    10441 gatattaaag tatactgttc attttgtggt aaaagccagg atgacgttaa aaaaattatc
    10501 gcaggaaata atgttttcat ctgcaatgaa tgcgtagctt tatcacaaga aattataaaa
    10561 gaagaattgg ctgaagaagt ccttgccgat ttgacagaag ttccaaaacc aaaagagctc
    10621 cttgatgttt taaatcaata tgtggttggt caagatcgtg ctaaacgtgc tttatcagtt
    10681 gctgtgtaca atcattataa gcgtgtctcg tttacagaaa gtcgtgatga cgatgatgtg
    10741 gatttacaaa agtccaacat tttgatgatt ggaccaacag gttcaggaaa aaccttctta
    10801 gcacaaactc tggctaaaag cctcaatgta ccatttgcta ttgcagatgc aacttctttg
    10861 actgaagcag ggtatgttgg agaagatgtg gaaaatattt tactcaaact gattcaggct
    10921 gctgactata atgtcgaacg agcagagcgt ggtattattt atgttgatga aatagataag
    10981 attgctaaaa aaggtgagaa cgtatctatc acacgtgatg tatctggtga aggtgtacag
    11041 caagcccttc ttaaaattat tgaaggtacg gtagcaagtg ttcccccaca aggtgggcgg
    11101 aagcacccta atcaagaaat gatccaaatt gatacaaaaa atatcttgtt tattgttggc
    11161 ggggcttttg atggcatcga ggagattgtt aaacaacgtt taggtgaaaa agttattggt
    11221 tttggtcaaa acagccgtaa aattgatgac aatgcttctt atatgcaaga aattatttca
    11281 gaagatattc aaaagtttgg gcttattcca gaatttattg gccgcttacc agttgtggca
    11341 gcccttgaac aactaaacac atcagatttg attcaaattt tgacagaacc aagaaatgcc
    11401 ctggtcaagc aatatcaggc ccttctttct tacgatggcg tcgaattggc atttgataag
    11461 gaagccttag aagccattgc caataaagcc attgagcgta agacaggagc acgtggtctt
    11521 cgttctatta ttgaagaaac gatgttagac attatgtttg aaattccaag ccaagaagat
    11581 gtcacaaaag tgcgtatcac caaggctgct gtcgaaggca agtcaaaacc cgttttagag
    11641 acagcttaag gagaaataat ggctgaagaa caagtcttaa atacccataa cgcctcaatt
    11701 ttattgagtg ctgccaataa atctcattac cccaaagatg atttgccaga aattgccctc
    11761 gctggacgtt caaatgttgg taaatcaagt tttattaata ccatattggg gcgcaaaaat
    11821 ttggcaagaa cctcaagcaa gccaggaaaa acccaactat taaatttttt caatattgat
    11881 gacaagctta gatttgtgga tgtccctggt tatggctatg ccaaggtttc taaaagtgaa
    11941 cgggccaaat ggggaaagat gatcgaagaa tacttaacca gcagagacaa tctgcgtgca
    12001 gtcgtgagtt tggttgattt acgtcatgcc ccttcaaaag aagatattca gatgtatgac
    12061 tttttgaagt actatgacat tcctgttatt gttgtggcaa ccaaagcaga taagattcct
    12121 cgtggcaaat ggaacaaaca tgagtctgtg gtcaagaaag ctttgaattt tgacaactct
    12181 gatactttta tagtattttc ttcggtagaa cgtattggta tcgatgattc ttgggatgct
    12241 attttagagc aggtctagcg ataggtgttg atttatctat gctaaaacat tctcagtaga
    12301 gggtgttttt cttatcgaaa acataaaaaa tcatagttag gtttaccgat tttacttgac
    12361 gccttaatga tgacatgcta gtattttata ttaaggagat gagtcatgac acaagtagcc
    12421 caagggcgct ctaaaggaaa gtcattgtta tgccatcaat gcaatgccct ttttgtattt
    12481 attatttttc ttatcttgct tgctgtactt tttggtgttc gatatcgtaa tagctctatt
    12541 gaaggtatct ggcgaacaac aagcattgat cagaagttag gcgatgattt tgctaaacgt
    12601 cttactggtt tgcatcagag ccctcttatt gatgatagtt tattaacgtc atctcagatg
    12661 atactaacag ttaaaaataa caacgttgac ttgagtttta gtgttcaagt tgaacgagac
    12721 atttttgtca aacgtttagc tgcttatcac caaaatgaat tattaaagac attaaaggaa
    12781 aatcatttag ttgttggtga cttgagtagt aaagaacgcc aaattattga gaatagtatg
    12841 ccagctagcc atgaattaga aatgatttta gatcaagctt ttgagaaatt agcttctcag
    12901 atcggaggca aatataatca aaaaacagga catttatctg ctgttgttct caaaggaaaa
    12961 gttaatcgta ttttgcacac cattgatatc aaagaagaag tagcagcggg acatacttct
    13021 ttttcaaaag gattgctcac acctaatggt tattttgatt acaccagatt tggaaagaaa
    13081 cttgagttat taggcgatga aaaaattatt tttaaaaaag cgctaaaaaa aaagtccgtc
    13141 aagtgtataa cgtagtcaca agacggattt tttcatgttt tgaaaattat tcaattgaag
    13201 aagtttatta ctgatctttt tgcctaatca ctagttgtcc ttcttctagg attgcctgaa
    13261 gttttttgac ttctggatgg tcaagataat aatcagttat acggtcacga atttcctgtt
    13321 cgataatacg tcgtaatgga cgagctccca tagcgtgatt gtaccctaaa tctatcaaat
    13381 gagctttagc gtcatcagca atcgttaaac taataccttt ttttgcggtg gtttgattta
    13441 cttcagctag cattaaatca acaatttctt ctaaattttc tttttgcaga tgattaaatt
    13501 taatgatact attaaaacgg ttcaaaaact ctggtctgaa ataaggtgca atgcgatcca
    13561 taatgttaaa cccagaagtt tccgtatctt gttggccaaa accggcattt gatgtagcaa
    13621 taataacggt atttttaaag ttaatagtat ttccttgtcc atctgtcaat cgtccatcat
    13681 ctaacacttg tagaagaagt gtaatgattt gaggatcagc tttttcaatt tcatctagta
    13741 agacaatagc ataagggtta cgacgaacac gctcagttag ggtattatta ttatcatcgt
    13801 agccaacata accagccgtt gtcccaatca attttgacac agcagtgcga tcgttatatt
    13861 cggacatatc aagtcgaata atagcatctt tggaaccaaa taaatcaagt gctaattgtt
    13921 tagctaactc tgttttacca acaccagttg gacccacaaa aaggaaagaa cctattggac
    13981 ggttaccatc atcaaaacct gctcgatttc gtcgaatagc acgtgctact gctgcaacag
    14041 cctcatcttg tccgatgaca tggcttttta agcggctgct aattcctttg agcctttcta
    14101 aatcattagt ccccatgtta gagacgggaa taccagttaa gcgctctaca gactctgcga
    14161 tatcattgac tgtagcagta acattttggc tttggttatg ttgctcaatt tgcttttgta
    14221 atgtttcaat ccgtgttttt gctgctaaag ctttttcaaa gtcttctttg gcgacagctt
    14281 tttcttgcct ctctttttgt ttagctattt cagtttcgag tgtttttaag tcagtgaccg
    14341 ggtgttgcgc agctaaatga gcagctgtca tatccagtaa gtcaattgct ttatctggca
    14401 ggctacgctg tgggatgtat tgaatagaat aatcaacagc agcttttaga acattatcag
    14461 gaagggtaat atggtggtgt tgttcataaa gattacgaat acccattaaa atgtgaaaag
    14521 tatcttcagc tgatggtgca ttcactttaa cttcattaaa gcgacgagcg agtgcagcat
    14581 tttttaaaat agtattgcgg tattcgtctt gagtagtagc accaatcagt gttaattctc
    14641 ctcgtgacaa ggctggttta agaatatctg caagtccctt tgagcctgaa tcactgcttg
    14701 tggcaccagc accaactatt tgatgaattt catcaaaaaa taagatgata ttcccagctt
    14761 cttttacttc ttgaatcaag ttttgaatgg tctcttcaaa ggaaccacga tactgtgtac
    14821 cagcttcaag actagaaata tcaattgata cgatttcctt gtttttaata gcagctggga
    14881 catctccatt aacaatggct tgagctaaac cttcaatgac tgcagtttta ccgacaccag
    14941 catctccgac aaggacagga ttatttttag ttcgtcgtgc caagatttca gcggtatctt
    15001 gaatttcttt gttacgacca atgacggggt caagatgccc ttgacgagct tcttgtgtta
    15061 agttagttcc gagttgagtc aatacactat cagctttagg tgttaacatt tgagaagttg
    15121 ttgctttggt tgtcgctaca gggagttgtc ctgtttgacg atagtgttgg aattcttcgg
    15181 gagttatttc gcgaccgtta acgagatagc ggggattttc gctacgataa ccacccatgt
    15241 ttgccattaa ttggttgaaa atatcatcca tatttacaaa aggatcttta ccagaaaaat
    15301 gtgtcatatc agttacctcg caataaaaaa tagactataa gatgtttgta agcagtcgca
    15361 gaatcgttgt taggatttca catgatagga aaccctttgc taataaaatg attcagacat
    15421 tatttttact
//
LOCUS       DAUVUY010000040        15205 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.040, whole genome shotgun sequence.
ACCESSION   DAUVUY010000040 DAUVUY010000000
VERSION     DAUVUY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15205
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.040"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            68..1726
                     /locus_tag="VOX58_001080"
     CDS             68..1726
                     /locus_tag="VOX58_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HES5878312.1"
                     /translation="MLIEMWSPVFKNHGEIRESICFHYGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSRDTANPDIILVCDKSYSP
                     TGETMDLGIFLNKLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYTFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTHQDKIDSDDIEKNQQKLQLRNTKLELESSLRDKQRRLKLLDISI
                     EFGLYPTESDLTELQQYFPDTNLKKLYEVEAYHKKLETILDSEFSTERESLIAEIDEL
                     ESQLTTLNQELQELGNIPNLSSEYLESYSKLTATINALKEQNEAYLKESGLSKEKSEA
                     DADLKRSTEDILLDLEVKINSKMREFNNILYPDIRKAPQINLNAHNSYSFYTPDDDGT
                     GTKFKGMIVFDLVMLYLTNLPALTHDSLLLSNVSYQATEALLKLYDQSRSLNKQVFLA
                     FDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGENSDEI"
     gene            1716..1925
                     /locus_tag="VOX58_001081"
     CDS             1716..1925
                     /locus_tag="VOX58_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5878313.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
     gene            complement(2028..3203)
                     /locus_tag="VOX58_001082"
     CDS             complement(2028..3203)
                     /locus_tag="VOX58_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HES5878314.1"
                     /translation="MYSKVIYRIGDVMFDFANNTFLAGLNPASLSLVAVYQSLESVIG
                     VLFNLFGGVIADSFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAIVLTNVILAFMS
                     AFSSPSYKAFTKEIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLD
                     GLSFLIAALLISFILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHKSIFIITVLSAL
                     VNFFLAAYNLLLPYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGFVNKELSSMRLI
                     LFLSLSGLMLMLAPPFYIMFHNAIILALSPALFSLFLSIFNIQFFSLVQKDVDNDFLG
                     RVFGIIFTITILFMPIGTGFFSVALNPNNSFNLFIIGSCITILSLVFRILFKKYNIR"
     gene            complement(3320..4477)
                     /locus_tag="VOX58_001083"
     CDS             complement(3320..4477)
                     /locus_tag="VOX58_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HES5878315.1"
                     /translation="MKITVVGIGYVGLSIGLLLAKEHDVTFFDIDNKKIDLINKRQSP
                     LKEAAINKLLCKAKNINATSSEELAYKDATFIILSLPTNLKFNKLDTSIIEISVSNIL
                     KINKKATIVIKSTVPIGFTEYLRNRFHYNDIIFSPEFLREGSTIHDQLYPSRTIVGNE
                     SRNSQLFLDILTDISVEKDSPSLLVGSSEAEAIKLFSNAYLAQKIAFFNELDTFAEMQ
                     NLDSKKIIEAMGYDQRIGNSHNNPSFGFGGYCLPKDIKQLEYHFKEIPAPIITSISES
                     NLLRKIHIAKMILNSSAKTIGIYRINSKKDSDNCRKSSTIDVAKLLKSSGKDVIIFEP
                     LINQKKFLGCPLINDFNEFIKYSDIIVANRIDDALRKCNSKVFTRDIFQYD"
     gene            complement(5170..6159)
                     /locus_tag="VOX58_001084"
     CDS             complement(5170..6159)
                     /locus_tag="VOX58_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HES5878316.1"
                     /translation="MFVIGIPTLNEADNISRLVKQIDEYAVNLGKEIIIINSDSKSTD
                     GTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAINHVPNFSGLILIDGDVVSMKK
                     MWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKFFKRDMPYQCISGDFGF
                     SRGLIKDLTLKCNWHKYTLGYGIDIFLTLTAILKSYKIKEIDLQSKIHKKSFEKIEKI
                     FLEVSQSFFETINDNSLNQDKLRLNINFESHSRQFIKSSDILSSNDIENLKLRALFLL
                     QEEKQYPHGLSEVEWDGILSNTINNIYRYSSEEHSLYLLPLYLLRVYNYLKYN"
     gene            complement(6214..7182)
                     /locus_tag="VOX58_001085"
     CDS             complement(6214..7182)
                     /locus_tag="VOX58_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HES5878317.1"
                     /translation="MFMMDKTVLYFGLVEISFDVPDFIFQKIKEQNNKIRYYSKSINI
                     LGSVKFINSNQQLIEYDGNGNILVYGRLKYFKEGISLYYIGEYLAECLLIEHSDTLLI
                     HAAAVYNPLSGHSILLLGDKGAGKTTVAIRLCIEHGYHLIGNDQVIFGSNSGILLTYA
                     GTSFFKIRRTAVLSDNLLFKLFSKFFNRSLQFNKASWDDKISIFPKELGIRTCNFSTE
                     ISKIYYIKTDKKEKQIYHSIWSDSLASLYLNELLGRHISGQVACFQSDNGKYFSTMPL
                     INYEKNNRVREQIVKGIFAKQKLFKITAATPAKIAEAIIENVKKNS"
     gene            complement(7166..8365)
                     /locus_tag="VOX58_001086"
     CDS             complement(7166..8365)
                     /locus_tag="VOX58_001086"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HES5878318.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSSIPLF
                     KIQKKAATKYLNRILPNLDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKAN
                     DTATMISYWPLTISEELALMIEGYFYKLDKNELPTPRFTKMGGDIKVMVVRNDLEYSI
                     RINFPLISKFFNNDIESQLYVDKHVEKIKKYIEQKYKNISFSIDYHYYLTTTGSCIDF
                     GEEGAVGRGNKTHGIISSFRPNTMEAPAGKNCTYFVGKVWGFLSDTIAKEIYEAFNTP
                     CQIIMQLNIGSKLYRPTHLFIQTEESVDQERVLEIVNRHLNNGKQNTNLILSTQHFIP
                     KTNVYDG"
     gene            complement(8375..9361)
                     /locus_tag="VOX58_001087"
     CDS             complement(8375..9361)
                     /locus_tag="VOX58_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HES5878319.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKMFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKKGIFVNAIYIKDLEKHDYSIEILS
                     CINQTVIKIISNLGSFAIREGKIEGGDAKTVRRVTIDDKTILFDPHPKLERYKSELVT
                     GTNTFQIHDDMMDTYISLLEGNAIPEDCYINNAIEITKLLINLYSECEKIYL"
     gene            complement(9354..10088)
                     /locus_tag="VOX58_001088"
     CDS             complement(9354..10088)
                     /locus_tag="VOX58_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HES5878320.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSGFFIEDNDVLCIA
                     SKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIKGLDYKWSENTSIKEALNE"
     gene            complement(10090..10926)
                     /locus_tag="VOX58_001089"
     CDS             complement(10090..10926)
                     /locus_tag="VOX58_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HES5878321.1"
                     /translation="MTFHITVNLDEISDKLDDSLKFLNRLSIKSCELRMINTKNIIQL
                     SDAELINLKERLQKYNITPISIASPLFKWNIEENHLESKLDLYGISANVTLNEQWLYV
                     RKIIFIAKFLNIKYVRIFGGIGCSVDDFLNNELFLNLLKETEIIFLIENELGTSVSTA
                     DDLLKIGHFIDEKSIKNLKIWFDIANYYIVEPQVDKVIPKLKKYIYYIHCKNYVNNDL
                     GIHYVELGKGIIDYKTIIHEIHEHFIDLIFSLEVHEKEIDKKAEVVRKSYLTLNEYIN
                     GG"
     gene            complement(10923..11849)
                     /locus_tag="VOX58_001090"
     CDS             complement(10923..11849)
                     /locus_tag="VOX58_001090"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HES5878322.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVDSVCLEDKIKHLINDPKCIGLNITLPYKREVMRYIDNLDRVAEIT
                     GSINNIYKSGNIITGTNTDWKGIYNTLVFFNISHITKCCVLGTGGTARAAIFTLQKLQ
                     ISPTVVYREPISENTRSLIKSFPDLKYITYKELADQQILNDIEIIINTTPVGMINYEE
                     SLPINFDHLDNVNYKDKYFMDVVFNPLHTKLHEYFGKRGAKIIDGLWVLIFQGIEAFS
                     LWSDKINGGKYFFSKDDVVLIHTFLEGKIKGK"
     gene            complement(11918..11989)
                     /gene="shp3"
                     /locus_tag="VOX58_001091"
     CDS             complement(11918..11989)
                     /gene="shp3"
                     /locus_tag="VOX58_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HES5878323.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            12069..12920
                     /gene="rgg3"
                     /locus_tag="VOX58_001092"
     CDS             12069..12920
                     /gene="rgg3"
                     /locus_tag="VOX58_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HES5878324.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     SLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYADHF
                     DKLVNKD"
     gene            complement(13173..>15205)
                     /gene="smc"
                     /locus_tag="VOX58_001093"
     CDS             complement(13173..>15205)
                     /gene="smc"
                     /locus_tag="VOX58_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HES5878325.1"
                     /translation="YAGVRAVLQSQKKLGGIIGAVSEHLSFDSDYQTALEVALGANSQ
                     HIIVTDEAAAKRAIAYLKKNRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAES
                     LVRYDDSLSAIIQNLLSSTAIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGG
                     TNRQSNTTFIKPELEQISEELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQ
                     ARLAEQRAQMAYQQLQEKQEDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKK
                     KSALTCDIDDIKENKDLVRQKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLK
                     QCQQNILKLESILNNNVSQDSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEA
                     RLEETAEKITKESEKNDTFIRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEV
                     TNSIDHLESAKEKLHHLQKTIRALGPINSDAINQYEEVHERLTFLTSQKTDLTKAKNL
                     LLETINSMDSEVKARFKVTFEAIRKSFKETFTQMFGGGSADLVLTETDLLSAGIEISV
                     QPPGKKIQSLNLMSGGEKALSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDF
                     LNRFDKDSQFIVVTHRKGTMAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
BASE COUNT         5097 a         2718 c         2053 g         5337 t
ORIGIN      
        1 tatctataat ggattgcttg tatgcattga acgctattga aatgtctaat gaaggagaag
       61 taaaattatg cttatagaaa tgtggtctcc agtatttaag aatcacggag aaattcggga
      121 atcaatctgc ttccactatg ggttaaacgt gattatgggg atggatttag ctgataactc
      181 tatcggcaaa tcctcctcac tattagccat tgatttcatc tttggaggta atagctatct
      241 aaaatcaatt gcggttaaaa aattaggtga tcatcctatc tatttctgct ttcaatttga
      301 gaaaaagttt tatttttcaa gagatacggc caacccagat atcattctag tgtgtgacaa
      361 gagctactct ccaaccggtg aaacaatgga cctaggaatt tttcttaaca aacttaagaa
      421 acgatatcat ttagattctc cagagctatc attccgatta gctatgagtg gcttctttag
      481 gattgcagga aaaagtaatc aaaataccga tttcccttta caagtttatt cgagccaaaa
      541 atcttctgaa tctattacta ctctcatcca actgtttaat ttgtatgata acattgcacg
      601 atacaaagaa cgtctgaaag ataagagcga ccagttaaca acttttcgta atgctcgaaa
      661 gtataccttc atctccaacc tggttggcgg taaaaagcaa tttgaagcaa atgtctcaga
      721 aataaaaaga ttggaatatg atttagcaca tctccaagac actcatcaag ataaaattga
      781 ctctgatgat attgagaaaa atcaacaaaa actccagctt agaaatacta agctggagtt
      841 agaaagcagt cttcgggaca agcaaagacg tcttaagcta ttggacatca gtatcgaatt
      901 tggcttatat cctactgaat ctgacttaac agagcttcaa caatacttcc cagatactaa
      961 tctcaaaaaa ttatatgaag ttgaagctta ccacaaaaaa ttggaaacta ttttagattc
     1021 cgaattctca actgaacgtg aatccctaat agctgagatc gacgaattag agagtcaatt
     1081 aacaactcta aaccaagaac ttcaagaact aggaaacatc ccaaatcttt caagtgagta
     1141 cttagaaagc tactctaaac taacagctac aatcaatgca ctaaaagaac aaaacgaggc
     1201 ctacttaaag gaatctggtc tatccaaaga aaaatcagaa gcggacgctg acttgaaacg
     1261 cagtacagaa gatatccttc tagatctaga agtaaaaatc aacagtaaga tgcgtgaatt
     1321 caataacatt ctatatccag atattcgcaa agctcctcaa attaacctca atgctcacaa
     1381 tagctactcc ttctatacac cagatgatga tggtacagga actaagttta aagggatgat
     1441 tgtattcgac ttagtaatgc tctaccttac taatctgcca gcactcacac acgattcatt
     1501 actcttgagt aatgttagct accaagcgac tgaagcactt ttgaagctgt atgaccagtc
     1561 aaggtcattg aacaaacaag tctttttagc cttcgataaa gccagctctt attctccaga
     1621 tgctaatcaa ctcttgtcag aaaatacggt attgagactc tctagtaacg gaaatgaact
     1681 ctacggtatt tcatggaaca aaggagaaaa ctcagatgag atttagttac aataaattat
     1741 ggaaacttct tattgataaa ggttggacca aaacaaagct caggcaagaa gctgggatta
     1801 gttcttccac gatcgcaaaa ttgggaaaag gggaaaatat cactactgat attttattgg
     1861 aaatttgtat tgcacttgat tgtaagattg aagatatagt agaaattgta gataataata
     1921 tctagaaata aaaaaagcac taagttgata agatgtgtaa gtcagatcaa cttagtgcct
     1981 ttactttgaa aaaaaataat tttctcaata atacttagta ctaactgtta acgaatatta
     2041 tactttttaa ataatattct aaatactaat gacaatatag taatgcacga accaataata
     2101 aaaagattaa agctattgtt aggatttaat gccaccgaga aaaagccagt cccaataggc
     2161 ataaaaagaa tagttattgt aaagataata ccaaacactc tccctaaaaa atcgttatca
     2221 acatctttct ggacaagaga aaaaaattga atattaaaga tggaaagaaa taaactgaat
     2281 aaagctggtg acaaagctaa aataattgca ttatgaaaca taatataaaa tgggggagct
     2341 aacattaaca tcagtcctga taaagataga aacaatatca atctcatact tgataattct
     2401 ttattcacaa aaccacttaa tattgcccca ataaatccac caattgcttc tgctgttaaa
     2461 aaagtgccat aaagtccagt tgaaatttct ccaaacattt gattactata gggcaacaat
     2521 aaattataag cggctagaaa aaaattaaca agtgcagaaa gaacggtaat aataaagatg
     2581 gacttatggc tgtaaacata tttaaatcct atttttaaat cgttaaatat ttctctgatt
     2641 gtcaccttct cctttatcac cacttcgtca ttaacaggta aaataaagga aattagtaaa
     2701 gcagctatta gaaatgatag tccatccaat agtaatacac cgtgtatacc aagtagctta
     2761 tataagaaaa ttgctaccat aggaactgtt actttgatta cagtacttgt tgtctctagc
     2821 aatgaattaa gttgtgatat actatctttt ttaacaattt cttttgtaaa tgctttatag
     2881 gatggactag aaaaagcact cataaatgcc aagataacat tagttagtac aatggcataa
     2941 accaaccatt gctcttttgt taggaacgag agtactaagc aagctgtgcc acataagata
     3001 tttgtagtaa taataatttt tttccgcttg aaactgtctg caatgactcc accaaataaa
     3061 ttaaatagaa cacctatgac actttctaga gactgatata ctgcaactaa tgatagagac
     3121 gctggattaa gcccagcaag aaaagtattg ttagcaaaat caaacataac atcgccaatt
     3181 cgatatataa ctttactata cactaaatat tgaatatttt tttcctattg ttcattttaa
     3241 agacctttaa aattttcaga tataataact tttaactgaa tttattactt aaaaatattt
     3301 ttttgcaaaa atgtttttgt taatcatact gaaaaatatc acgtgtaaaa acttttgaat
     3361 tacattttct cagagcatca tctattctat tggcaactat aatatccgaa tatttaataa
     3421 attcattaaa atcattaatt aaagggcacc ccaaaaactt tttttggtta attaagggct
     3481 caaagatgat aacatcctta ccactgcttt ttagaagttt agcaacatca attgtagaag
     3541 atttcctaca attatccgaa tcttttttgg aattaattct ataaattcct attgttttag
     3601 ctgagctgtt caaaatcatt tttgctatat gaatttttct taataaatta gactcactta
     3661 tactggtaat aattggtgct ggaatttctt taaaatgata ctctaattgc ttaatatcct
     3721 taggaagaca gtacccacca aaaccgaaag aaggattatt gtgcgaattt cctattctct
     3781 ggtcatatcc catagcctca ataatttttt ttgagtccaa attttgcatt tcagcaaacg
     3841 tatccaactc attaaaaaaa gcaatttttt gtgccaagta tgcattcgaa aataacttta
     3901 tcgcttctgc ttcagaagag ccaactaata aagatggcga gtctttttca accgatatat
     3961 ctgttagtat gtctaagaat aattgagaat ttctagattc atttccaact atagttctcg
     4021 aaggatacaa ttgatcatga atagttgatc cttccctaag gaactcaggt gaaaaaatga
     4081 tatcgttgta gtgaaatcga ttccttaaat attctgtaaa accaattgga actgtcgact
     4141 ttattacaat tgtagccttt ttgtttatct ttaaaatatt acttacagaa atttcgataa
     4201 tggaagtatc aagcttatta aattttaggt tggttggcaa agacaagatt atgaaagtcg
     4261 catccttata tgctaattct tcagaagaag ttgcattaat attttttgcc ttacataaaa
     4321 gtttgtttat agctgcttct ttaagagggg attgcctttt atttattaaa tcaatttttt
     4381 tattatcaat atcaaaaaag gtgacgtcgt gttcctttgc aagtaggagc cctatcgata
     4441 atccaacgta tccaatgcct acaactgtaa ttttcatgtt aacctctatt ctaaaacttt
     4501 ttataaatca atttttttgt tagaaagaat ataaaattga tttttatacc taaatgagat
     4561 gatataaatt tttggatgtc tacttaagaa ttcagatgta tcagaaaaag aacctagata
     4621 ttcccccttt ttttgttcgt caacaacacc aataaaatgt aaagaaatta aagaaatact
     4681 agtaagctta gtctttagtt cttgtctcca gcctgaaata ttgagataga attcattaaa
     4741 aatgaaccct agtctataat agatcacact atcatcttca ctgacaaaga ttggtagatt
     4801 tctcttttta tattcctcaa agtcatcttc gattgcagtc ctactagaat tttttttcta
     4861 accatttcca acgtactcat tagcgtctca atcatactgg tcccctttct aagaaaaaac
     4921 agattttcat taagtgtttt tatcttttaa actcaacaca aatatcaaaa atccaaactg
     4981 gcatctagcc cactaaagtc aattaaaata atccatcaaa aaattaaata ttatcgttac
     5041 tactcaaagt atcaaaaata ttataaatag atttacttac atactgtatt tattatttag
     5101 ataagctaaa catattgaaa tatattccac caatcacact tcttctcaag attaataaaa
     5161 attgaaaact tagttgtatt ttagataatt gtatactctc aataaataaa gtggtaataa
     5221 atataacgaa tgttcctcac tactatatct ataaatattg ttaattgtat tagacaaaat
     5281 tccgtcccat tctacctctg acagcccatg tggatactgt ttttcttcct gtaataaaaa
     5341 caacgctctt aactttaaat tctcgatatc attgctggat aggatatcac tggacttaat
     5401 aaactgtctt gaatgacttt caaaatttat atttaaacgt aacttatctt gattcaaaga
     5461 attatcatta atagtttcga agaaactttg tgaaacttct aaaaaaattt tttcaatctt
     5521 ctcaaagctt tttttatgaa tctttgattg taaatcgatt tccttaattt tgtatgactt
     5581 taatatagct gttaaggtta aaaaaatatc tatcccgtaa cctaatgtat atttgtgcca
     5641 attacacttc aaagttaaat cttttatcaa tcctcttgaa aatccaaaat caccagagat
     5701 gcattgataa ggcatatcac gtttgaaaaa tttcactaac atgggataaa taaaatggtt
     5761 tgtcgcattt ccttcaaaag attttctagc ataatttggg attataagat catttccaga
     5821 ttcaattgct ataaacatct tttctaacca catttttttc attgagacaa cgtctccatc
     5881 aatcaaaatt aaaccggaga aattcggcac atgatttatt gcatattcga aaatatttct
     5941 cacattataa ccttttcctt tagcttctga tactatcgaa actttggtgt tataagtttt
     6001 ggtttctaaa aaaatctgcg gagttccatc tgtactttta ctatccgaat taataataat
     6061 aatttctttc cctaaattta cagcatactc atcaatttgt ttaactaatc tactgatatt
     6121 atctgcctca tttaaagttg gaatgcctat cacaaacata attactctta ctcctttttc
     6181 aatacttcat attaatcccc aaattcacac acactatgag tttttcttaa cattttctat
     6241 aatcgcttct gcaatcttag ctggcgtagc tgctgtaatt ttaaataatt tttgtttagc
     6301 gaaaataccc ttcactatct gttctcgtac ccgattattt ttttcataat ttatcaaagg
     6361 cattgtagaa aaatattttc cattatctga ttgaaaacat gcaacctgtc cagaaatatg
     6421 ccttcccaat agctcattta gatatagact tgctaaacta tcactccaaa ttgaatgata
     6481 aatttgcttt tctttcttgt ctgtctttat ataataaatt tttgagattt cagtagaaaa
     6541 attacaagtt cgtattccca actcctttgg gaaaattgaa attttatcat cccaacttgc
     6601 cttattaaat tgtaatgaac ggttaaaaaa tttgctaaat aatttaaaca gtaaattgtc
     6661 cgacaaaaca gctgtccttc ttattttaaa gaaagatgtc cctgcataag ttagtagtat
     6721 tccagagttc gaaccaaata ttacttggtc attaccaata aggtgatacc catgttctat
     6781 acacagcctt attgcaaccg tagtttttcc agcaccttta tctcctaata gtaaaattga
     6841 atggccagat aaaggattat aaactgctgc tgcatgaatt aataaggtgt cactatgttc
     6901 aatcaagaga cattcagcta agtactctcc aatgtagtat aatgaaattc cttctttaaa
     6961 atactttaat cttccataaa ctaaaatatt tccattgcca tcatattcta taagttgctg
     7021 attgctgtta ataaatttta cactacctaa tatatttatt gatttgctat aatatctaat
     7081 tttattattc tgctctttta ttttttgaaa tataaagtca ggcacatcaa aagaaatttc
     7141 aaccaaccca aaatataaaa cagttttatc catcataaac attggtttta ggaataaaat
     7201 gttgagttga caagattaaa ttagtatttt gtttaccatt atttaaatgc ctgttaacaa
     7261 tttctaatac tctttcttga tcaacacttt cttctgtctg aataaataaa tgggttggtc
     7321 tatacaattt tgatccaata tttaattgca taatgatttg acagggagta ttgaatgctt
     7381 cataaatttc tttggcaata gtatcagaca aaaatcccca aacctttcct acaaaataag
     7441 tacaattttt tccagctggt gcttccatag tatttggcct aaaacttgat atgataccat
     7501 gagttttatt acctcgacca actgcacctt cttctccgaa atcaatacaa gaacctgtag
     7561 tagttaaata ataatggtaa tcaatagaaa agcttatgtt tttatacttt tgttcaatat
     7621 atttcttaat tttttctacg tgtttatcaa catacaactg actttcaata tcattgttaa
     7681 aaaatttact tattaaagga aaatttattc taatagaata ttctaaatcg ttccttacga
     7741 ccattacttt aatatcacca cccattttag taaatcttgg cgttggtaat tcattcttat
     7801 ctaatttata aaaataccct tcaatcatta atgctaattc ttcagaaatt gttaatggcc
     7861 aataacttat cattgtagct gtatcatttg cctttggtac atctagatac tccggtaaat
     7921 cttctattgc ttcaggcgag aaccaattag tttttgtagt aaaatctgag gtcaatgtct
     7981 caaattctac ataattttca acatctaaat taggtaaaat tctatttaaa tatttagttg
     8041 cagccttttt ctgaatttta aaaagtggaa ttgaactatt aaaacatttt ttgctcgccc
     8101 tacctagaaa aataatttta attggttcaa taaagtcact tccaccaaaa tcctgaactg
     8161 aatgtccccc tcggataata atcttatcaa aattatgatg tggaataaca ccaaacttgt
     8221 ccaaacaata aagtgagtaa tcaatttcaa tttgctcagc aattccatct gctaaggtat
     8281 caggatgccc taatccttta cgttctacta cttcgtatga taaatgactt gggtcataaa
     8341 agccgtttgt aatttttaaa tccatagtta cacctcataa ataaattttt tcacattctg
     8401 aatataagtt aattaataat tttgttatct caattgcatt attgatatag caatcttcag
     8461 gaatagcatt tccttctaac aaactgatat acgtgtccat catatcatcg tgtatttgaa
     8521 acgtatttgt cccagtcacc aattcactct tatacctctc gagtttagga tgtggatcaa
     8581 atagtattgt tttgtcatca atggtaactc ttcttactgt tttagcatca cctccttcaa
     8641 tcttgccctc tcttattgca aaacttccca aattactaat aattttaatc actgtttgat
     8701 taatacaaga taatatttct attgaataat cgtgtttctc taagtctttt atataaatag
     8761 cattcacaaa gatccctttt ttgaattctt ctaatgaaat tcctaaataa gaaatgacag
     8821 caatgatatg aggaatctct attccatagc ccaatatctc tttatctaaa aatctcccat
     8881 tctcgttatc ctcaatccta tctttagaaa aattaactgt tatttccttg attttatcta
     8941 gagagaaaca atctacttgt aacagttttt ccaaaatttt cgaaaagaag tattgttcac
     9001 tggcaaacat tttttttgct agatgtgggt gactttctac tagcgacttc atttcttcta
     9061 aatcttctaa cctgttacac agtggttttt caatgctaat ataagaatag acataccttg
     9121 ttataacggt tttcaatagc gtaaggtggt gatttgtcgg agtacaaatc tcaatagtaa
     9181 aatattgtcg tgataaatca tccaacacat cactatttgt attaaggtat ttcatgctgg
     9241 agaatttcaa atctttgaca tatacctcat atccgcgtga tagccacttg tacgctttaa
     9301 ttttcccaat ttttccaaaa ccaatgatta gaatattctt acagtcctga tttctactca
     9361 tttaacgctt ccttaatact agtattttca gaccatttat aatccaagcc cttaatgagg
     9421 acagctggaa ttccagttcc acgttgcccc attactaagc cagcacaagc tgcaatcata
     9481 tcacacaaag tctcttcttg gaattttatt gtctctcctt gtgaatctat tacttctgtt
     9541 tttctcaaag gatgaatacc ataaatacca attgctactt gagtagcacc tcttttatcc
     9601 tctctaccat cactatctgt tataacaact gcaacatttt ttccaagtga ttttttaaga
     9661 tattccgaaa ttctttttgc agaagcatcc ggattattag gtaaaagaaa tacttcatct
     9721 actgattttc tatcgattcc accacttgtc aactttaaac cattttccaa gcgacaacca
     9781 atgtaatttt ttttgatatc taaacgtgac agatctctat tgactaaatt tagcataatt
     9841 tcaattattc taggatcttt acgaggaata ttcctgtgga tttcttttgc tacatcactc
     9901 acttgtatct cgtttaaaga catcacctgg ccttctgcga tagatacaac tttagaagct
     9961 atgcaaagca catcattgtc ttcgataaaa aatcctgaag ttttaatgct ttcttcaata
    10021 agcttttcta tactatcccc ttctttcaaa taaggaatat tctttaaacc aaatatactc
    10081 aaattcattt tagcctccat taatatactc atttagtgtc agataagact tcctcactac
    10141 ttctgccttt ttgtctattt ctttttcatg tacttctaaa ctaaaaatta agtctataaa
    10201 atgttcatgg atttcatgaa tgattgtttt ataatcgata attcctttac ctagttctac
    10261 ataatgtatt cctaaatcat tgttaacata gttcttacag tgaatatagt aaatatattt
    10321 tttcaatttt ggaataacct tgtctacctg aggttcaact atgtaataat ttgcaatatc
    10381 aaaccagatt tttagatttt ttatagattt ttcatctata aaatgtccta tttttaacaa
    10441 atcgtctgca gtgctcactg aagttcctaa ctcgttctca attaaaaaga taatttcagt
    10501 ctcttttaac aaattcaaaa atagttcatt attcaagaag tcatctactg agcacccaat
    10561 cccaccaaat attcttacat atttgatatt taaaaatttt gcaataaaaa taattttcct
    10621 tacataaagc cattgttcat ttaacgtaac attagctgaa ataccgtata aatcaagttt
    10681 actctctaaa tgattttctt ctatattcca tttaaaaagt gggcttgcaa ttgaaattgg
    10741 agtgatatta tacttttgta accgttcttt taaattaatt aactctgcat cactaagttg
    10801 tataatattt ttagtgttta tcattcgtaa ttcacaactt ttaattgata atctatttaa
    10861 aaatttcaat gaatcatcta gtttgtctga tatttcatca agatttactg taatatgaaa
    10921 agtcattttc cttttatctt cccttctaga aatgtatgaa ttaggacaac atcatctttt
    10981 gaaaaaaaat atttaccacc attaatttta tcactccaca atgaaaatgc ttcaattccc
    11041 tgaaaaatca aaacccataa accatcaatt atttttgctc ctcttttacc aaaatattca
    11101 tgaagtttcg tatgtaaagg attgaaaact acatccataa agtatttatc tttataatta
    11161 acattgtcca aatgatcaaa atttataggt aaactttctt cataatttat cataccaact
    11221 ggtgtagtat ttattatgat ttctatatca tttaaaattt gttgatcagc caattcctta
    11281 tatgtaatat actttaaatc agggaaactc tttatcaaac ttcttgtatt ctcactaatt
    11341 ggctccctgt atacaactgt tggtgagatt tgtagttttt gtaaagtgaa aatagcagct
    11401 ctagcagtac cgccagtacc taaaacacaa cattttgtta tatggctaat attaaagaaa
    11461 actaatgtat tataaatacc tttccaatca gtattagttc ctgtaattat atttccagat
    11521 ttataaatat tgttaataga tccagttatc tctgctactc tgtctaaatt gtcaatatac
    11581 ctcattacct ctcttttata aggtaaagtt atatttaatc caatacattt tggatcattt
    11641 atcagatgtt tgattttatc ttctaaacac actgagtcta ccaaaatttt tttatgttta
    11701 tattcatcca gaactcctat gttgtgattt agatattcaa ataattcatc tgatatagaa
    11761 tgttcaactg gattaccaag caatgcagaa tacataaatt tcatcctcct acgtttaatt
    11821 agtttactac taacataaag tacaagcatt acacagagct aaccattacc tataagtctt
    11881 tagagtatct aaagacttag gtgtacacct aagtaactta cccaccaaca ataatgataa
    11941 tatccattgc taaaattaaa atcggcaaaa attttgaaat tttcttcata agcggacttc
    12001 ctttcagttt ttatttattg tacaataaaa aaaaacggaa ttgatttttg ttgggaaagt
    12061 gggaaaatat gaaatcaaaa cttggtgcca cacttagaaa agttcgaaaa ggaaagcaaa
    12121 ttagtctgtg cttcgtggca gatgaacacc tttcaaaatc acaaatctct cgttttgagc
    12181 gcggggagtc agagatatcg tgtatccgac tgatcaatat tctagacaaa ttacacatta
    12241 ctttggatga gtttcttatt ttacatgatg aagactatac caattccgaa acatttgcta
    12301 acctggtaca gtatattcgt aagcaatatt catcacaaaa tatcaataat atagcatgtt
    12361 tactttccga cacttctgat tacactctga attcttttga gaaaacaatg gtaaagtcca
    12421 ttttacacac aatggattca agcatcattc catcagatga tgaattactt caactaacag
    12481 attatctctt taaagttgaa aaatggggct actatgaaat ccttttgttg ggtaactgtg
    12541 tacgaacaat taactacaac tcctacttcc tattgacaaa ggagatgtta aataattaca
    12601 tttactcttc acggaacaaa actaataaac gaattgtcac tcagcttgct ataaattgtt
    12661 tcattcttag tatcaataag gaggaatttt ctaattgctc ctatttaatt gatgagataa
    12721 aatcattatt agataatgaa ttaaacttct atgaacagac agtttttctc tatgcaacag
    12781 gttactttga atttaagcgc caattagaaa ctggagttga aaagatgaaa caagccatac
    12841 aagttcttga tattttaaat gaagacaagt taaaattaca ttacgctgac cattttgata
    12901 aactagtcaa caaagattaa gtgaaatcat tctttacctt ttaacgaaat gccaatcaag
    12961 agtgtctcaa taaaaaatca tctactctga caacttttct cttttaccta tcgacagcta
    13021 aaaagccttg gaaactgcta tttccaaggc tttctttgta ctctattgta tcaattagct
    13081 tattccgatt tctgttgcta ttagtttaat catgaaatct cctaacttat gaccgaacat
    13141 ccaaaaaaac agaaggaact cccttctgtt ttttaattgg tcatctcttg agcttctttt
    13201 aattttacag atacaatttt agagaccccc gattcctgca tggtaattcc ataaatacta
    13261 tctgctgcag ccattgttcc tttacgatgg gtcacgacaa taaattgact atctttgtca
    13321 aagcgattga ggaaatcccc aaagcgctta acatttgctt catcaagagc cgcttctacc
    13381 tcatctaaaa tcacaaacgg aatcgtttta acacgtataa tagcaaacaa taaagccagt
    13441 gctgacaaag ctttctctcc gcctgacatc aagttaagag attggatttt cttaccagga
    13501 ggctgcacag aaatctctat accagcagac aagagatctg tttccgttaa aaccaaatca
    13561 gcagaacccc caccaaacat ctgagtaaag gtttccttaa aactttttcg aattgcctca
    13621 aaagtcactt tgaaacgagc cttcacttcg ctatccatac tattaatagt ctctagcaaa
    13681 agatttttag cttttgtcag atctgtcttt tgacttgtta aaaaagtcaa acgctcatgc
    13741 acttcctcat actgattaat agcatcacta ttaattggtc ctagtgcacg aatagtcttt
    13801 tgtaaatggt gtaatttttc tttggcactt tctaagtgat caatactatt tgttacctcc
    13861 tttgcgtctg ctagtgtcat ttgaaaatct tctgataaac ttttagcata ggctcgtaag
    13921 cgattagcca cttgctctaa atgtgtctct aacttggttt gacgacgaat aaatgtgtca
    13981 tttttttcgc tttctttggt tattttttct gctgtttctt ctaagcgggc ctcatagtcc
    14041 tctatttcaa atcttaattg gactaaacgt ttttgagcac cagacttatg ctctgtggca
    14101 tcttgtaact gtttttgcca ctggggtagt ctttgaatgc tgtcttgact tacgttgttg
    14161 tttagtatgc tttcaagttt gagaatattt tgctggcatt gcttcagctg agttcttaga
    14221 cgagattgat tagcttgctc aaatttcttt tcattaagta aatctctttc ttgcaaacga
    14281 gcttgagata aagcttggtg aatgttttgt gttttttgcc taaccaaatc tttgttttct
    14341 ttgatatcat caatatcaca agttaaggca ctttttttct tagcaatagc tgttaatgct
    14401 tcttcaatac gagcttgctc tgccaataaa gattcatcag aatgagtcgt ctgactttga
    14461 tcaagggcag ctaacaaagc ctttgaatct tcttgttttt cttgcaattg ctgataagcc
    14521 atttgagctc tttgttcagc taagcgtgcc tggtcaccgg ctaatttcaa ttgtgtcagt
    14581 tcttcttttt ttgctatcaa atcgctttgc aaggcagcta cttctttttc agttattttt
    14641 aattgctcaa ctaatcgcgt taattcctca ctgatttgtt ctaactctgg tttgataaaa
    14701 gttgtattgc tttgtctatt agttcctcct gaaaaagaac cacctggtcg taattctgtt
    14761 ccatcaagag taacaattct taccttatat cctaataagc gcgctgcaat attagcttga
    14821 tcaatagttt caaagatagc agtactagat aaaaggtttt ggataatagc tgaaagacta
    14881 tcatcatagc gaaccaatga ttctgcagtt cccagataac cttcacaagt cgctaattga
    14941 cgatgatagt gttctgataa tgatctagcc tttatagtag ttaaaggtaa aaaagttgct
    15001 cgtccttggc gatttttttt caaataagca attgctcttt ttgctgcagc ttcgtctgtt
    15061 acaataatat gctgactatt agcacctaat gcaacttcaa gagctgtttg gtaatcagaa
    15121 tcaaaagaaa ggtgttcact gactgcacct atgatccctc caagcttttt ttgtgattga
    15181 aggacagctc gtactcccgc ataga
//
LOCUS       DAUVUY010000041        15035 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.041, whole genome shotgun sequence.
ACCESSION   DAUVUY010000041 DAUVUY010000000
VERSION     DAUVUY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.041"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            564..653
                     /locus_tag="VOX58_001094"
     CDS             564..653
                     /locus_tag="VOX58_001094"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HES5878326.1"
                     /translation="MIIDYCNNKRIKIKRKGLSPLQYKTKSFT"
     gene            complement(691..1614)
                     /locus_tag="VOX58_001095"
     CDS             complement(691..1614)
                     /locus_tag="VOX58_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878327.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1607..2677)
                     /locus_tag="VOX58_001096"
     CDS             complement(1607..2677)
                     /locus_tag="VOX58_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878328.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2686..3612)
                     /locus_tag="VOX58_001097"
     CDS             complement(2686..3612)
                     /locus_tag="VOX58_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878329.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3612..5114)
                     /locus_tag="VOX58_001098"
     CDS             complement(3612..5114)
                     /locus_tag="VOX58_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878330.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYEKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMAGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5179..7149)
                     /locus_tag="VOX58_001099"
     CDS             complement(5179..7149)
                     /locus_tag="VOX58_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HES5878331.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTVGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKVEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDSAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYENAKEKWMKAKAKSNAKYAEKLADHVEK"
     gene            7480..8712
                     /locus_tag="VOX58_001100"
     CDS             7480..8712
                     /locus_tag="VOX58_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HES5878332.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDTKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFDGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            8880..10061
                     /locus_tag="VOX58_001101"
     CDS             8880..10061
                     /locus_tag="VOX58_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HES5878333.1"
                     /translation="MTKRIILTIFTFICFSVMPLVQAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(10213..11631)
                     /gene="sufB"
                     /locus_tag="VOX58_001102"
     CDS             complement(10213..11631)
                     /gene="sufB"
                     /locus_tag="VOX58_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HES5878334.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(11624..12103)
                     /locus_tag="VOX58_001103"
     CDS             complement(11624..12103)
                     /locus_tag="VOX58_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HES5878335.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(12090..13316)
                     /locus_tag="VOX58_001104"
     CDS             complement(12090..13316)
                     /locus_tag="VOX58_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HES5878336.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(13347..14609)
                     /gene="sufD"
                     /locus_tag="VOX58_001105"
     CDS             complement(13347..14609)
                     /gene="sufD"
                     /locus_tag="VOX58_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HES5878337.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPELELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKTSANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQETAERLVIRGF
                     LGAVIAEIPIPSVHQEIIKVLDEKLLNR"
     gene            complement(14704..>15035)
                     /locus_tag="VOX58_001106"
     CDS             complement(14704..>15035)
                     /locus_tag="VOX58_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878338.1"
                     /translation="GGEKKRNEILQLLMLEPKFALLDEIDSGLDIDALKVVSKGVNEM
                     RGKDFGAMIITHYQRLLNYITPDLVHVMMDGRIVLSGDAALATRLEKEGYAGIAQDLG
                     IEYKEES"
BASE COUNT         4606 a         3041 c         2764 g         4624 t
ORIGIN      
        1 ttgcccgcgt tgtaaaatga agtgcaacaa aaaagagaaa aagccttgaa ctacacccca
       61 aacgttagac aaaaaaatct aacgttcggg gtatttttta tgaaattaag tgatgaaagt
      121 acgaaataga catcgttaag aaagaaaaaa atacctacta tccaccagag ataaagcaag
      181 aaataatgga taacgttttg atagaaggcc cgccacaata ccaagtatca cttgtttatg
      241 gcttgttaca tgcaggaact ctcccaagtt gaatagtaca atataagaaa aacagttata
      301 ctgttgagaa aacaagagca agaccatcac aaacaggacg taaaccaaaa gaaacctgaa
      361 gaaatgacta aattagagcg gcttcaagaa gaaaatgaat atctcagaac tgaggttgct
      421 tacttaaaaa aaccgaggaa ctccgtttga aagaagcatc tctgtaaaga agaagacaaa
      481 aaaaccatta taaaaaatgg acaaagacga gttctgatta gattttcttt taagaacggc
      541 tgtattgact cgttctacct accattatca tcgattattg caacaataaa cgtatcaaaa
      601 taaaacgaaa aggacttagt cctctacaat acaaaactaa atcctttact taaaatagag
      661 tagccaactt ttggggtcag tgcatctctt ttacaattct ttttgatatt tttcaatttc
      721 tgcttggttt gcccaaacaa agtgatttgg tttgatttca accattgatg gcttatctaa
      781 tgtataatca tgttggtctg gatgatagac aacaagttct ttttgacgct ctaaaattgg
      841 atctgggata ggcacggctg ataacaaaga ttgggtgtag ggatgaattg ggttattaaa
      901 cagttcttct gtttctgcaa cttctacaat aaccccttta tggataaccg caatacgatc
      961 tgaaataaag cgaacgactg aaagatcatg ggcaatgaag agataagtca aacctttttc
     1021 ggcttgcatc cgtttgagaa ggtttaaaac ctgtgcgcga acggaaacgt ccaaagctga
     1081 aatcggttca tcagcaataa cgaattcagg gttcattact agggctctag cgataccgat
     1141 ccgctgacgt tgacctcctg aaaattcatg agggtagcgc gtcaaatgct ctgatagcaa
     1201 accaacttcg gccatcatgt tcttaatttt ttctttacgt tcttcctctg ttttaaacag
     1261 attaaagtta taaagacctt ctgaaatgat gtagtcaacg gttgcccgtt cattcaaact
     1321 agcagcggga tcttggaaaa tcatttgaat tttacgaatg agctcattgg cttctgattt
     1381 tgattttctg ccattaatta ctttcccatc gtataaaatt tgacctgagc tagtatcgtt
     1441 caaaccaata atagcacgac caattgttgt tttaccactc ccagattctc caactaaaga
     1501 aaaggtttct ccttttttaa taaagaaatt agcattttta actgcaacaa attttttctt
     1561 tccttctccg aaggaaattt ctaggtcttt tacttcgact aatttctcag acatttcctt
     1621 cctcctgttg tgacattttc cttaagattt tttgatgcaa atcttgaatg acttctggtt
     1681 tttgaacttt tggagcctct gggtgtaaaa gccatgtttt ggcccaatga gtctcagata
     1741 cgttaatcgc cggaggtgct ttttcaaagt ctaaaaccat agcatattct gagcgaagtg
     1801 caaaggcatc tccgataatt ggtgaataaa gtgatggagg cgttcctgga atagcgtaaa
     1861 gttcaccaga ttcatctgcc aactgcggta agctagacag caaactccat gtataggggt
     1921 gccttggatc atagaaaatc tcttcgactg ttccaaattc aacaatttct cctgcataca
     1981 tgacagccac tttatctgca atgcttgcca caacacctaa atcgtgcgta ataaagataa
     2041 tggtgaaatg atattctcgt tgaagcgact tcaataactc aacgatttga gcttgaatag
     2101 tcacatcaag ggctgttgtt ggctcatcac agataagaat atctgggcga caagctaaag
     2161 caatagcgat aacaatacgt tgacgcattc ctcctgaata ctcaaatggg taatcttcaa
     2221 agcgtttttt ggcatttggg atacccactt tattcatgta atcaagggcc atttctttag
     2281 ctttggcatg acttactttt tggtgcttaa taatcacttc tgtgatttgg ctaccaatag
     2341 ttttaatggg actaagactg gtcattgggt cttggaaaat cgttgcgatt tttgagccgc
     2401 gaatctttgc ccactcttta tttgttttta aatctgtcaa ttcttgccca cgatagacaa
     2461 ttgaaccatt agcaatgcgt ccattagact ccaacatccc tgtaaaggtc tttgttaaaa
     2521 cagatttacc tgagcctgat tcccctacaa aagcaaggac ttctccttca acaagttcca
     2581 gtgagacgtt acggatagct gttaaaacac gatcacgcac atcaaattct accaccacat
     2641 ctttggcagt taagattaca ttattttctt ttgtcatttt aactcctatc tatgtgaacg
     2701 tgggtcactg gcatcagcca agttttgtcc gacaatgtat agtggtaacg atactaaaat
     2761 caatgttact aagggaatcc aaaagaggta ggcatttgtt gttaagttgc ttgaataatt
     2821 agcaatcaaa cgtcctaaac ttggagtggt ggttggtaaa ccaatcccaa agaaggataa
     2881 gaaggcctca gaagatacat aaactggtag catttgtgac aacatagtca tgataactga
     2941 aaccaattga ggcaggaggt tcttaacagc aatcttgtac attggtgttc ccaaagtttg
     3001 actagcaagg ttgtattcta aatcacggta acgcaagatt tgaacacgga tggagtaggc
     3061 gacaccaatc catccagtga tacagaaagc tagaatcaaa ttccaaaatc ctgcacctaa
     3121 tgaataggtc aaaacaatga taataagcat agaagggata tttgagataa tgttataaat
     3181 ttcaatcata actttatcaa atgctttaga aactccccat atggctccta acactacccc
     3241 aatggtgata ttaattagtg tcgctataac tgagattaaa atagaattac gtgccccata
     3301 ccaaacacca tcaaacagag attgcccatt tttgtcggtt ccaaaccagt actctgcatt
     3361 tggccaaata taacgctttg aaaagtcatt gatattacta acgtcattaa agtcgtagtt
     3421 ggcaaacatt ggataaataa agctcatcat caagactgtc actaaaatca cgagcataaa
     3481 gactgtagat tttttagaaa aaaactgacg aaacactgat ttccagtaag aataagcagg
     3541 ggtatcaatc acttcggagg cttcactatc gcgctcaaca aatcgaaatt tagatttatc
     3601 aatcgattcc attacttacc tccttttttt gttgacaatt taatacgtgg atcgacaagg
     3661 gtcatgacaa tatctcctag taaaagtgaa acaattgaca agacagtgaa gataaaggtt
     3721 aatccaacaa tcatagaatt gttagcagat ttaatagaat caattaacat tttacccata
     3781 cccgggaagg caaagacagt ctctgttaag gttgcaccac caatagcaag gataatactt
     3841 gcaggaaccc ctgacacaat tgggaccatg gcatttttga agagatgccc tctgtaaatt
     3901 tcagattctg ataatccttt tgaacgcgca aaacgtaccc aatcactggc ctgaaggtca
     3961 acaagatagc gacggaacca gataaccgta gttggaatat ttaaaattcc taaaataagg
     4021 gctggtaaaa tataagattt aggatcagat gcccctagca ttgggaaact atctggtaaa
     4081 ccagccatgc cacctaggaa gcgaacaaca tagataacag caatacttgg taaagcaagc
     4141 atgaaggtca tggtcgctgt tgagaaacgg tcaaaatagg tatttttaaa ccgagccata
     4201 aataaaccaa gaggcaagcc aacgatatag gaaaacataa caccaaaaaa accaattaca
     4261 aaagaattgt gaatcattga tgggtcgaca taattattga tggttttagt gtaggaatct
     4321 cccttaccat agtttacctt ggtgatatca tccaatgatt ttggattttt gtagctacgg
     4381 ctatagatat ccactgacga tttcttagtg acacctgatg ggaaggtcac atcttgaaga
     4441 gctgttcgtc cttgcccttg tgaaataacc tgaagaacag gaatattgct gtatgttggg
     4501 taagatgttc ctaagtttag ggtaacaaag ttttggtgaa gataaggaaa ttttccgtta
     4561 gtgtagagga gatatttatg ttttgtcccc gaaccaacaa gtgaccagcc aactgattta
     4621 tctttttcta gacgaatata cctagctaat tttggattgt ctttatcctg aatcttccaa
     4681 gggtgatcaa taacaactag atttgagaaa aagttccaaa cacgttcgta aataggaata
     4741 ttgcgaattg cataaaactg tttgctagtg gggaagcgtt ttagctgcca gccattgcca
     4801 agagaatcaa tatacttctc atagatagct ttgttagcac tagttgcttc tgtcgttaca
     4861 ctactatcta ctttagaagc attatctttt aattctttgc ttgaaaaata attgacataa
     4921 cccatacgtt gaaaagttag attttcatac gctacttttt tatctggtgt tgttgtcatt
     4981 ttattataat tagggtcttg tttaaagata aggctggtag gtactagagt atatacgatg
     5041 gtgtatgtta aagccgttac caaaactacc gacactaacg atcgcatgat acggtttaaa
     5101 atgtattttt tcatagttat cctcaatagt tcaaaagaac ttcctcacat aaacgagaaa
     5161 gttcttttga acatcgtttt atttttcaac gtgatcagct aacttctcag catattttgc
     5221 atttgactta gcctttgctt tcatccattt ttcttttgca ttttcgtatt gttttgctgt
     5281 gacaggtttg tcttgaagtt tcattccttt atatgctaga ggacctttag accctgccca
     5341 agagaagccc ccgctaaatg gaacggcttt agtaactcgt ggagtgccac ccaatgccac
     5401 aactggaata tctacggcac tatctgtaag gtaggcttgt gcttttgcgt aagctttata
     5461 gcgcgcatcg ttgtcgtctg taattgctgc tgcttcatca aggagagttt ggtaagtatc
     5521 aaggcctgca gctgccacaa catccttatt ttgacctgct ttgattccaa gtttttggat
     5581 aacagatcca ccacctactg gactcatgat gtcaaggtag gtccgtggat cttggtagtc
     5641 tggtccccac catgatgaaa tgatatcgta gtcttgttgt tctggggtct cagcatagaa
     5701 gccttgggct tcgtgagttg atgtttctgt ttcaagaaca ttgacaatga cattttcttt
     5761 accaagagat gcttcaacag attgtttgaa agactgggct tcctgaacag ttgctgcgtt
     5821 tgcctggtca acagggtaat ctaattgaac tgggaaggtt acaccttcag ctgtcaaagc
     5881 ttctttggct tttgcaaatt caacttttgc tttttcagga ttatagaaac catcttgagc
     5941 atcagctaag ttaacgtctt tccattcatc accaagtttt gccatttcct tttcaacttc
     6001 tgaaccaaaa tcactttctc caacggttac aaaagttggt ggcacaagca tgttacgtaa
     6061 ggcttttgtt ttggcatctt gacctgcagt ttgtgcttgg aatgacgctc ggtcaaaagc
     6121 aaactgaata gcttgacgaa aatccttgtt gttaagagct ttcttaccgg catcttgttg
     6181 tgcagggtct ttcttagtgt ttttgaaaga agtacggttc aaattccatg ttaaatgacg
     6241 gatatctcca gtcaacattc cgtaagtaat attatcagca tagtttttct tagctgattt
     6301 gtaggtaggg tcatttgggt aaagtcgtgc aacgctgaac tcacccttgt caaagttctt
     6361 gtagaacgaa cctgggtctg aaccatctga gtaagtcaat ttaacagatt ctatcccaac
     6421 attcttagca tcccagtagt tttcattttt atggaattcc atagatgatt ttgaggtgaa
     6481 ggcgctcaag aagtaagcac cattaacaag gattgatgat ggatcggttg taccaaaatc
     6541 tttacctttt gacttcaaaa atttcgcatt aactgggaaa agcacactat aagttgtttt
     6601 tgaattccag tagctttcag gcttgttcaa agtatactga acagttttat cgtcaagggc
     6661 tttgacacca acttctttaa aatctacttc accattttgg taagccttta agttttttat
     6721 tgaatcttca acaacgtaaa gagcatctga tttatcgtca accgcgtgct tcaaaccagt
     6781 cacaaaatct tctgctgtta ctggggcata ttcttcacca tcagccgtat accaagagac
     6841 accatcgcga agagtataag tataagtcag accgtctttt gaaaccttcc aatctttagc
     6901 aagtgatggt actaaattac catactcatc gttttccaaa agaccatcaa ccatttgtgt
     6961 tatcacatca gtcgttccac cgccattagt caaaatataa tccaatgatt ttggatcgtt
     7021 aacaaaaacg tacttgtagg tttttgtagc ttctgagcca cctgaagcat ttttattacc
     7081 acaagctgcc aaagcggata ctgacaagat cgcaacactt acagctgcca accatttact
     7141 tttcttcata aaagtcaccc ctaaacttta tttttcattt tttggcaatt gctaaatgaa
     7201 attggttaat tgaagacatt atatcataaa actttcttaa agaaaataat gaaatgaaaa
     7261 attatctgaa tatttcgtag aattttcttt attttataca tttatctata tgaaagcgtt
     7321 atactccttt tttaatgagg tttcaattag tttaataaag attgtcattc tctcatggta
     7381 gcgttttatt gcatcactac tcttgtgagg taagtcatta ccttagacaa aaatatggta
     7441 taattaaaaa aataatactt gagtaaggat aatctataaa tgatcaaacg attaatttcc
     7501 ctagtggtca tcgccttatt ttttgcagca agcactgtta gcggtgaaga gtattcggta
     7561 actgctaagc atgcgattgc cgttgacctt gaaagtggca aagttttata cgaaaaagat
     7621 actaaagaag ttgtcccagt cgcctcagtc agtaagctct tgacaaccta tctggtttac
     7681 aaagaagttt ctaagggcaa gctaaattgg gatagtcctg taactatttc taactaccct
     7741 tatgaactca ctacaaacta tactattagt aacgttcctc ttgataagag aaaatatacc
     7801 gttaaagaac ttttaagtgc gttagttgtt aataacgcca atagccccgc tattgcttta
     7861 gctgaaaaaa taggcggaac cgaacccaaa tttgttgaca aaatgaaaaa acaattaagg
     7921 caatggggca tttccgatgc aaaggtcgtt aattcaactg gcttaactaa ccatttttta
     7981 ggagctaata cttatcctaa tacagaacca gatgacgaaa attgtttttg cgccactgat
     8041 ttagctatta ttgccaggca tctcttatta gaatttccag aagtactgaa attatctagc
     8101 aaatcctcca ctatttttga tggacaaacc atttacagtt ataattacat gcttaaaggc
     8161 atgccttgtt atcgagaagg cgtggatggt ctttttgttg gttattctaa aaaagccggt
     8221 gcttcttttg tagctactag tgtcgaaaat caaatgaggg ttattacagt agttttaaat
     8281 gctgatcaaa gccacgagga tgatttagct atatttaaaa caaccaatca attgttgcag
     8341 taccttttaa ttaattttca aaaagtccag ttaattgaaa ataataaacc agtaaaaacg
     8401 ttatctgtct tagacagtcc tgaaaaaact gtcaaacttg tagcccaaaa tagtttattt
     8461 tttatcaaac caatacatac aaagaccaaa aataccgtcc atattactaa gaaatcatcc
     8521 acaatgatcg cacctctatc aaagggacaa gtcttaggta gagcaaccct tcaagataaa
     8581 catcttattg gacagggtta tctggatact cctccttcta tcaatcttat ccttcaaaaa
     8641 aacatttcta aaagtttctt tttaaaggtc tggtggaacc gttttgtgag gtatgtcaat
     8701 acctctttat agaataacac aatgcttttt gtatgacatt gactgacaga gtacttctgc
     8761 agtctttttc atttttattt aagcaaacta ggccaatcct aacaattttt caaaaaaact
     8821 tcacattgta ttagcaaagt gatatactga aaatgcatta catttaaagg agtcggaaat
     8881 tgaccaaaag aattatacta actattttta cctttatttg cttttcggtg atgcctcttg
     8941 tccaagccga agatgtcatg gatattacaa ggcaagcagg ctacacagtt agtgaggtta
     9001 atcgaccaaa atcttctatt gtcgttgatg caaatagttc agatattctt tggcaagata
     9061 atattgatat tcctagggat cctgctagta tgtctaaaat gtttacgctt tacattttat
     9121 ttgaagagct cgctaaggga aaaattacga tggacactac tattacggca actccaaccg
     9181 atcaagctat cgctaacatt tatgagatta gcaataataa tatcgtcgct ggagtagctt
     9241 atcctattcg tgatcttatt actatgacag ctgtgccttc atcaaatgca gcaactgtta
     9301 tgattgccaa ttatttatca aacaatgatg cttctgcgtt tatcgatcgt attaatgcca
     9361 cagccaaaca attaggcatg actaacactc atttttcaaa cgctagtggt gcagcagcac
     9421 aagcttttca aggttattat aatcctacta aatatgattt atctgcttca aatattacga
     9481 ccgctcgaga tttgtccaag ttactctatg ccttcttaaa aaaataccct gaaattatct
     9541 cctttacaaa taaatctgtt gtgcacacta tggtaggaac gccatatgaa gaagaatttc
     9601 acacctataa ccactctctc ccagacaatc aatttggtat gaaaggagtt gatggattaa
     9661 aaacaggttc tagtcccagt gctgctttta acgccatgat aactgccaaa agaggcaaaa
     9721 ctcgactcat tactattgtc atgggggtcg gagattggtc agatcaaaac ggagagtttt
     9781 atagacatcc tttcgttaat gccttgactg agaaagggtt taaagatagt aagaccttat
     9841 ccaaaaaagc acgccaaaaa ctcgaaaaat tagtgccaca aaccaaaaaa gaaacatcac
     9901 ctaaacagca gcacttcaaa gccactaaaa agcaatcgta cttggaaaga gtcgaagatt
     9961 ttatgaatca caaccacact ttcttgctta tttgcttagc tatctttata attactatcc
    10021 ttttactaag cctcgttgtc tttgcaatgg gacgccaata atgaattatc aaaaacctct
    10081 gtcaaagcac agaggttttt gatatgttat cttacgtgtt caaggcaact aaaaactaac
    10141 cacttggggt tagtactaca actcataaaa tcctatctca aagatctttc agatggataa
    10201 gagaaaatgc aattaaccga cagaaccttc catctcatag gaaatcaatc gattgagttc
    10261 gaccgcatat tccattggca attctttggt aaaaggctcc acaaatccca tgacaatcat
    10321 ctctgtggct tcgctttctg ataagccacg gctcatgaga tagtagagtt gttcttcaga
    10381 aatcttagac actttagctt catgttctaa cgccacctgt gaattatgaa tttcgttaaa
    10441 tgggatcgta tctgatttag aaatatcgtc catcaaaata gtatcacatt caatatggga
    10501 aacggatttt ttagattgct tgttaaaggt cacttggcca cgataatcta ctttaccacc
    10561 agacttggca attgattttg acacaattga tgatgatgta tggggagcat tgtgaatcat
    10621 tttagcccct gtatcttggt gttggcctgc gttagcaaag gcaatagaca gcatggtgcc
    10681 acgcgcacct ggcccatcaa ggtaaacaga agggtacttc atggtggttt tagctcctag
    10741 attgccatca atccattcca ctgttgcatc cgtaagggcg cgtgcacgtt ttgttactag
    10801 attatacaca ttgtctgacc agttttgaat ggtggtataa cgcatgtatg caccgtcaag
    10861 cgcaaagatt tcaacgatag cagcatgtaa gctgttactt gaataagtgg gagctgtaca
    10921 tccttcaaca taatgaacac ttgctccttc atcaacaata atcaatgtcc gctcaaattg
    10981 accagtattt tcattgttaa tgcggaaata agtttgcaaa gggatatcta ccttgacccc
    11041 tttaggaaca taaataaagg taccaccaga ccaaactgct gaattgaggg ctgctaattt
    11101 gttgtctgtt ggcggaacca gtttggcaaa gtattgtttg aacaaatcag gatattcttt
    11161 gagggcggaa tcggtatctg taaagataat ccctagcttt tcaaattcac ccttcatgtt
    11221 atggtaaacc acttctgact catactgagc tgatgcccca gcaagataag cacgttctgc
    11281 ttctggaatt ccaatacgat caaaggtttc ttttattttt tctggaacat catcccaaga
    11341 acgagctggc ttatcagatg ctttttgata gtaaatgata tcatcaaagt taatatctga
    11401 taagtctgct ccccaggttt gcatcggcat tttattaaag gtttccaagg attttaaacg
    11461 aaactccaac atccactcag gttcattttt ggcagctgat agttcgcgaa ccactgcctc
    11521 actcaatcct tttcctgtag aataaatggg ctctacatca tcgtgaaatc caaattggta
    11581 gtcccctaag tcaattggct ttggctctac tttctcattt atatcagaca tttttccctt
    11641 cctttacatt ttggtccgtg aggtgctgag cattggcact tcgtttaatg gcttccttga
    11701 gagcattcca agctagggta gagcatttga tacgctgtgg aaattttgca actcctgcca
    11761 acaattctgc ttcacctagc tctttttgag caggattttc ctgtccttgt accatctctg
    11821 aaaaaatatc agctaacgcg agtgcttctt ctttactttt accaataaca gcatctgtca
    11881 tcatgcttga tgaagctgtg gaaatggtac agccgttgcc tgcaaaagca atatcttcaa
    11941 ttttatcttc gtcaaactta acggtcaaag aaatcacatc accacaagtc ggattattca
    12001 gttgaacagc ctctacgcca tctagttgcc catgatgatg tggacgtttc gaatggtccg
    12061 ctaccacagc catgtataga tggttcagtt tagagagtgc cattgaaaaa ctcctttgct
    12121 tttagaattg cttcgactag acggtcacaa tctgccttgg tgttatagat ataaaaactt
    12181 gctctaacag ttgctggtac acctaaataa ctaagaagag gttgggcgca gtggtgccct
    12241 gctcttactg caacaccttc atagtccaag gctgttgcca agtcatgagg atgcaaatca
    12301 tccagattaa aagaaatcag accagatctt gcactaggct ggcttggtcc atatatggta
    12361 agcccctcaa tagcttctaa tttcggcaag acataggcta ttagttctgc ttcatgcgca
    12421 tgtatatcag ccatgcctag tctctgaagg taagaaatgg ctgcgcttag cccaatagca
    12481 ccagctatgt gaggtgttcc tgcttcaaac ttccagggca attctttcca agtggcctct
    12541 tgttcgtaaa caaaatcaat catttctccg ccaaattcaa gaggctccac ttgattcaaa
    12601 agctcttctt tgccgtaaag aacacccaaa cctgttggcc ccaacatctt atgagctgaa
    12661 aaagcaaaga aatcacaatc caagtcttga acatcaatag ccaaatgtgg aaccgactgg
    12721 gcaccgtcaa caacaaggta ggctccttta gcatgtgcca gcttggcaat ttctttaatg
    12781 ggattgatgc aaccaagaac attggagaca tgtactaggc taacaaaacg tgtttttgtc
    12841 gtcaatttgt ttgccaaatc gtccatgtca agttggccat cttttaaata aacatagact
    12901 aaccttgctc ctgttttttg acaggcttgt tgccaaggaa tgatattggc atgatgctcc
    12961 ataatcgaaa tcaacacctc atcttctggc gtcaagacct gctctgcaaa ccgagcaacc
    13021 caattcaaac tggttgttgt tcctctggta aagagcactt ccttacttga tttagcatga
    13081 ataaagtcag caacctgctg gcgactggcc tcatatttgt gcgttgcacg ttcagccaag
    13141 gtatgaactc ctcggtggac attagcatta tcttcttgat aataggattg caaagcctca
    13201 agaacaagcg ccggtttttg tgtggtggcg gcattatcca aataaacaag gggttcatca
    13261 ttgacttgtt gatttaagat ttgaaagtct tgtttgatgt cttttgcgtc tagcaatact
    13321 aacctctttc tttttggcag taggtcttaa cgattaagca atttctcatc taaaacctta
    13381 ataatctctt ggtggactga tggaatagga atttcagcga taactgctcc taggaatcct
    13441 ctaataacca atcgttctgc tgtttcttga tccagtcctc gactcatcaa gtaatacata
    13501 tcttcagggt caacctgacc gatagaagct gcatgacctg ctgttacttc attttcatca
    13561 attaagagga ttggattggc atcggctctt gcttggtcag aaagcatcaa aacacggctt
    13621 tcttgttgag catcagctcc cttagcgcct tttagaatat gaccaatccc gttaaacgtt
    13681 aaggtgccac gttccaaaat cacaccatgc tgtaaaatat gaccgaccgt acgttgacca
    13741 tagttggtca cgcgcgtgtc aataccttgt acctgacgac cacttgaggc tgcaacaact
    13801 ttcaaatcag cttgtgagcc ctgaccaatc aaatcactgt caaaatcagc aatgacattg
    13861 ccttcattca tcacagctaa ggcccaatca atgttggcat ccttctctaa acgtcctcga
    13921 cggctaatat aggttgtcac tgaaggacct aagcggtcga tagccgagaa tttaatctgg
    13981 ctgcctgctt gggcaatcac ttctacactg atattagcgc tggtcttttg agtggcattg
    14041 ccaatagatt caaaacgctc taaataggtg aacttacttt cttttcctgc aatcactaga
    14101 acatgcttgt taaaaggaac gtcactgtca ctatcttgta agaaaatagc ttcaatagga
    14161 gttgtgattt ccaagtgatc aggaacgtag agcacggctg cgctattaaa ataagcagtg
    14221 tggtaggcag ctagtttgtc ttcatcaaaa gctaatgcct gaccaaaatg agcttcaatt
    14281 acttctggga tttcctcaag cgccgtataa aaatcactga aaacaactcc cttgtcaatt
    14341 aacgccattg gtaactgttc taagactgtt tgcgtgccta cctgaacaag ctttgggtta
    14401 tctccaatag ctataaaatc tggaacacta gctagacttt cattttctgt taaggtacca
    14461 tctcctagat tccaacggtg aaatttaacc ctttcgatgg ttggtaactc taattctggt
    14521 atggcttcta atgccgctaa acgccgttct tgcagccaag caggctcagc gtgggcttgc
    14581 gaaaaagcta ctagtttttc ttttgtcatc tgtcaatcct ttgtctctgg tttttaaatt
    14641 ggctgggtat atagaacatc acctagcttt ttgtagaaag ctaaagtaat tgtctccaaa
    14701 aacttaactt tcttccttat actcgattcc cagatcctgt gcaatacctg cgtagccttc
    14761 tttttcaaga cgagtagcca aggcagcatc accagataag acaatacgac catccatcat
    14821 aacatgaact aggtctgggg taatgtaatt caacaaacgt tgatagtggg tgataatcat
    14881 agctccaaag tctttcccac gcatctcatt aaccccttta gagactactt ttagggcatc
    14941 aatatcaaga ccagaatcaa tttcatctag caaagcaaat ttaggctcta acatgagcaa
    15001 ttgaagaatt tcattgcgtt ttttctcccc accag
//
LOCUS       DAUVUY010000042        15034 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.042, whole genome shotgun sequence.
ACCESSION   DAUVUY010000042 DAUVUY010000000
VERSION     DAUVUY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15034
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.042"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..143)
                     /locus_tag="VOX58_001107"
     CDS             complement(<1..143)
                     /locus_tag="VOX58_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878339.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFG"
     gene            complement(153..851)
                     /locus_tag="VOX58_001108"
     CDS             complement(153..851)
                     /locus_tag="VOX58_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878340.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(851..1222)
                     /locus_tag="VOX58_001109"
     CDS             complement(851..1222)
                     /locus_tag="VOX58_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HES5878341.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            1407..4517
                     /locus_tag="VOX58_001110"
     CDS             1407..4517
                     /locus_tag="VOX58_001110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HES5878342.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELADCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQDLTEAGLKEKGLWKEPYQSRLLRELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQIYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSNYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIVEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKIMDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            4597..5610
                     /gene="pfkA"
                     /locus_tag="VOX58_001111"
     CDS             4597..5610
                     /gene="pfkA"
                     /locus_tag="VOX58_001111"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HES5878343.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            5673..7175
                     /gene="pyk"
                     /locus_tag="VOX58_001112"
     CDS             5673..7175
                     /gene="pyk"
                     /locus_tag="VOX58_001112"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HES5878344.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICCETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            7393..7950
                     /gene="lepB"
                     /locus_tag="VOX58_001113"
     CDS             7393..7950
                     /gene="lepB"
                     /locus_tag="VOX58_001113"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HES5878345.1"
                     /translation="MVKRDFIRNILLLLIVIIGAILLRIFVFSTFKVSPETANTYLKS
                     GDLVTIKKNIQPKYKDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYL
                     EKMKAHYLNHAPFGTLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLI
                     NASQIKGLVTFRVLPLSDFGFVEVE"
     gene            8126..9940
                     /gene="glmS"
                     /locus_tag="VOX58_001114"
     CDS             8126..9940
                     /gene="glmS"
                     /locus_tag="VOX58_001114"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HES5878346.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLATTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            10136..10471
                     /locus_tag="VOX58_001115"
     CDS             10136..10471
                     /locus_tag="VOX58_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HES5878347.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            10578..11219
                     /locus_tag="VOX58_001116"
     CDS             10578..11219
                     /locus_tag="VOX58_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HES5878348.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            11229..11858
                     /locus_tag="VOX58_001117"
     CDS             11229..11858
                     /locus_tag="VOX58_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HES5878349.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKSDAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            11874..12710
                     /locus_tag="VOX58_001118"
     CDS             11874..12710
                     /locus_tag="VOX58_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HES5878350.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKGLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQEKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANI
                     KS"
     gene            13079..13852
                     /gene="cfa"
                     /locus_tag="VOX58_001119"
     CDS             13079..13852
                     /gene="cfa"
                     /locus_tag="VOX58_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HES5878351.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKSFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
     gene            complement(14222..>15034)
                     /locus_tag="VOX58_001120"
     CDS             complement(14222..>15034)
                     /locus_tag="VOX58_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HES5878352.1"
                     /translation="SKRVKSSALVAASKDNLADAVTSIGTSIAIIAASLHLPVIDHIA
                     AMIITFFILKTAFDIFMESSFSLSDGFDSRHLKKYEKAILEIPKIVAVKSQRARTYGS
                     NVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQFSIYDIDIHVEPAMILEEEIFDNVA
                     KKLYRYEKLILSKVPDYDHYIAKSFQLVDANGQTVNYEQFLNQEIYYPSNFNHFQIES
                     ISQKTMLVTYQLNGNQHTSIWRRHESWSLLFHQITPIAKRQLHHTHYRIVKM"
BASE COUNT         4757 a         2476 c         3068 g         4733 t
ORIGIN      
        1 ccaaatagtc catcactatt actttgagca aacagtttta tcgtaaatga caaaatggca
       61 gcgatggcaa taacgaaagc attaagcgca aagtaccatt ttccaataga tctaaattcg
      121 tattttaata attttccaaa catgatatcc tcctaaacct tataagtttc cctaaagagg
      181 gaatcaatgg attgctggta ttcttgtctt agatcatcag cattgccaga taagaaaagt
      241 cttccttgtt tcaagaaaat gacttcgtca aggataggtt caatgtccga aatcaagtgg
      301 gttgagataa taacagaagc attttcacaa taactattaa tgatggtttt taggatgtag
      361 tctcttgcgg cagggtcaac tccaccaata ggttcatcaa gtacatagag tctggcttta
      421 cgactcatca caagaattaa ttgtactttt tctttgtttc ctttggatag agttttgaag
      481 cggtcttcag ggtctagttc taaatctctt agaaggcttg ttgctttgga tttgtcaaag
      541 tcgctgtaaa aatcactaaa gaactccaac atgtccttaa ttctcatatt ttcattgaga
      601 taagtagtat ctggtaaata agaaataatt ttttttgtct caacacaggg acggtagcca
      661 tcaatgacaa tttctccttt atttggttgt aaaaggccat taatgagttt aattaaagtt
      721 gttttaccac ttccatttgg acctaataat ccgataattt taccgttcgg gatagtaatg
      781 gttaagtcat caatagcttt tttttcacgg taagattttg aaacatgatg tagttgcaat
      841 aggtgtgcca ttattgtgaa tcctccttta aaaagctcgt caaaacaggg ataatctcag
      901 tgtgactaaa tcccattttt gtcatattgg tgataaatga ttctaattca ctaattgcta
      961 actctcgtct tttacgagca attaattttt gatcgtcagt tacaaaacgg ccagcagtac
     1021 gttgcgaata aaccattcct tcccgttcaa gttctgtaaa cgctcgttgc atggtgttag
     1081 gattaacacc tgcgatttct gcatattcac gaacagttgg aagttgatca ccactcttga
     1141 tttcttggct aataatttgc atcatgacat gttgtgctat ctgcgcataa ataggagatt
     1201 tttcatcaaa tttccaagac atgtggtctc ctttctgagt aatatagata tttgtactaa
     1261 ttaaatggta cagaaaaaaa aagggaatgt caagttttat gccatttttc actctttgag
     1321 aagtagacgt agattagaga gaaaattgaa gcaagaaaac ctttttcata gtataatagg
     1381 gaacaagata accaaaggag gaacttatgt ttgctcaact tgatactaaa actgtatact
     1441 catttatgga tagtttaatt gacttaaatc attattttga acgagcaaag caatttggtt
     1501 accacaccat aggaatcatg gataaggata atctttatgg tgcttaccat tttattaaag
     1561 gttgtcaaaa aaatggactg cagccagttt taggtttgga agtagagatt ctctatcaag
     1621 agcggcaggt gctccttaac ttaatcgccc agaatacaca aggctatcat cagcttttaa
     1681 aaatttccac ggcaaaaatg tctggcaagc ttcatatgga ttacctctgc caacatttgg
     1741 aagggatagc ggttattatt cctagtaagg gttggagcga tacattagtg gtcccttttg
     1801 actactatat cggtgttgat cagtatactg atttatctca tatggattct aagaggcagc
     1861 ttatacccct aaggacagtt cgttattttg cgcaagatga tatggaaacc ttgcacatgt
     1921 tgcatgccat tcgagataac ctcagtctgg cagagacccc tgtggtagaa agtgatcaag
     1981 agttagcaga ttgtcaacaa ctaaccacct tctatcaaac acactgccct caagctctac
     2041 agaatttaga agacttagtg tcaggaatct attatgattt cgatacaaat ttaaaattgc
     2101 cacattttaa tagagataag tctgccaagc aagaattgca agacttgact gaggctggtt
     2161 tgaaggaaaa aggattgtgg aaagagcctt atcaatcgcg cttactgcgt gaattggtca
     2221 ttatttctga catgggcttt gatgattatt ttttgattgt gtgggattta cttcgctttg
     2281 gacgcagtaa aggctattat atgggtatgg gacgtggctc ggcggcaggt agtctagtgg
     2341 cttatgctct gaacattaca gggattgatc cagttcaaca tgatttgcta tttgagcgct
     2401 ttttaaacaa agaacgttat agcatgcctg atattgatat cgatcttcca gatatttacc
     2461 gttcagaatt tctacggtat gtccgaaatc gttatggtag cgaccattcg gcgcaaattg
     2521 tgaccttttc aacctttggc cctaaacagg ctattcgtga tgttttcaaa cggttcgggg
     2581 ttccagaata cgaactgact aatctcacta aaaaaattgg ttttaaagat agcttggcta
     2641 ctgtctatga aaagtcaatc tcttttaggc aggttattaa tagtagaact gaatttcaaa
     2701 aggcctttgc tattgccaag cgtatcgaag gaaatccaag acaaacgtcc attcacgcag
     2761 ctggtattgt gatgagtgat gataccttga ccaatcatat tcctctaaaa tcgggcgatg
     2821 acatgatgat cacccagtat gatgctcatg cggtcgaagc taatggcctg ttaaaaatgg
     2881 attttttggg gttaagaaat ttgacctttg ttcaaaaaat gcaagagaag gttgctaaag
     2941 actacgggtg tcagatcgat attgcagcca ttgatttaga agacccgcaa acgttggcac
     3001 tttttgctaa aggggatacc aagggaattt tccaatttga acaaaatggt gctattaatc
     3061 ttttaaaacg gattaagcca caacgttttg aagaaattgt tgccactacc agtctaaata
     3121 gaccaggggc aagtgactat accactaatt tcattaaacg aagagaagga caagaaaaaa
     3181 ttgatttgat tgatcctgtg attgctccca ttttagagcc aacttacggt attatgcttt
     3241 atcaagaaca agttatgcag attgcacaga tttatgctgg ttttacgtta ggcaaggccg
     3301 acttgttaag gcgtgccatg tctaaaaaaa atctacaaga aatgcaaaaa atggaagaag
     3361 actttattgc ttctgctaag cacctaggga gagctgaaga aacagctaga ggacttttta
     3421 aacggatgga aaaatttgca ggttatggtt ttaaccgcag ccatgccttt gcctattcag
     3481 ctttagcttt tcaattggct tatttcaaag cccattaccc ggctgttttt tacgatatca
     3541 tgatgaatta ttctagcagt aactatatca cagatgctct agaatcagat tttcaagtag
     3601 cgcaagttac cattaatagt attccttaca ctgataaaat tgaagctagc aagatttaca
     3661 tggggctgaa aaatattaag gggttgccaa gggattttgc ttattggatt gttgagcaaa
     3721 gaccatttaa tagcgtagag gattttctca ctagaactcc agaaaaatat caaaaaaagg
     3781 ttttccttga gcctctgata aaaataggtc tgtttgattg ctttgagcct aaccgtaaaa
     3841 aaattatgga caatttggat ggtttactgg tatttgttaa tgagcttggt tctctttttt
     3901 cagattcgtc ctttagttgg gtagatacga aagattactc agcaactgaa aaatattctt
     3961 tggaacagga gatcgttggg gttggcatga gcaagcatcc tttaattgat attgctgaga
     4021 aaagtaccca aacttttact cccatttcac agttagtcaa agaaagcgaa gcagtcgtac
     4081 tgattcaaat agatagcatt aggatcatta gaaccaaaac aagtgggcag caaatggctt
     4141 ttttaagtgt gaatgacact aagaaaaagc tcgatgtcac actttttcca caagagtatg
     4201 ctatttataa agaccaatta aaagaaggag aattctatta cttaaaaggt agaataaaag
     4261 aaagagacca tcgactgcag atggtgtgtc agcaagtgca aatggctatt agtcaaaaat
     4321 attggttatt agttgaaaac catcagtttg attcccaaat ttctgagatt ttaggtgcct
     4381 ttccaggaac gactccagtt gttattcact atcaaaaaaa taaggaaaca attgcattaa
     4441 ctaagattca ggttcatgta acagagaatt taaaggaaaa acttcgtcct tttgttctga
     4501 aaacggtttt tcgataaaaa tggacaatta gtgaagtatt ttgcaaatga atgtgctata
     4561 ataagaccgt taaattaatt aaaggagtac cagaaaatga aacgtattgc tgttttaacc
     4621 agtggcggcg acgctcctgg tatgaacgct gctattcgtg cagtcgttcg taaagcaatt
     4681 tcagaaggaa tggaagttta tggtatcaac cgtggctatg caggtatggt tgatggcgac
     4741 attttcccac tgggttcaaa agaagttggt gataaaatct cacgtggtgg aactttctta
     4801 tactcagctc gttatccaga gtttgctcaa cttgaaggtc aattggctgg tattgagcaa
     4861 cttaaaaaac atggtattga aggtgttgtt gttattggtg gggatggttc ttatcatggc
     4921 gctatgcgct tgacagagca cggcttccca gcggtaggta tcccaggaac aatcgataac
     4981 gatattgcgg gaactgacta cactatcgga tttgatacag cagttaacac agctgttgaa
     5041 gctattgata aacttcgtga cacctcatca agtcacggac gtacttttgt ggttgaagtg
     5101 atgggtcgca atgcgggtga tattgctctt tgggcaggta ttgctagtgg tgctgatcaa
     5161 atcattgttc cagaagaaga gtttgatatt gaaaaagtgg catcaacaat ccaatatgat
     5221 tttgaacaca aaggtaaaaa tcaccacatt atcgtcttag ctgaaggtgt tatgagcggt
     5281 gaagcttttg ctcaaaaact gaaagaagct ggcgataaga gtgaccttcg tgtgacaaat
     5341 cttggccaca ttcttcgtgg gggttcacca actgctcgtg accgtgtcat tgcttcttgg
     5401 atgggttcac atgctgttga gctacttaaa gaaggcaaag gtggtttggc tgttggtatt
     5461 cacaatgaag aacttgttga aagcccaatc cttggtactg cagaagaagg tgctttgttc
     5521 agcttgactg aagaaggcaa gattattgtt aataatccac acaaagctcg tttagacttt
     5581 gcagctttga accgttcatt gtcacaataa gtttagttaa aattgttaaa tcgtcaatta
     5641 ttgacaaaaa aataagggag tttcagagta aaatgaataa acgcgtaaaa atcgttgcaa
     5701 cacttggtcc tgcggttgaa atccgtggtg gtaaaaaata cggagaagat ggctactggg
     5761 ctggtcaact tgatgttgaa gaatctgcaa aaaaaatcgc tgaattaatt gaagctggtg
     5821 cgaatgtttt ccgtttcaat ttctcacatg gtgaccacaa agaacaaggt gaccgtatgg
     5881 caactgtccg ccttgcagaa gaaattgctc gtcaaaaagt tggtttcctt ttggatacta
     5941 aaggtcctga aatgcgtacg gaattgtttg cagatgatgc taaagagttc agctatgtta
     6001 ctggagaaaa aatccgtgta gcgactacac aaggtattca atcaactcgt gatgttattg
     6061 ctttgaacgt tgctggtagc cttgacatct acgatgaagt agaagtaggt catactattc
     6121 ttatcgatga tggtaaactt ggtttgaaag tgatcgacaa agatatcgca acacgtcaat
     6181 ttatcgttga agtagaaaac gacggtatta ttgctaaaca aaaaggtgtt aatatcccta
     6241 acactaaaat tcctttccca gcacttgcgg aacgcgacaa tgctgatatc cgttttggtc
     6301 ttgaacaagg acttaacttt attgcaatct catttgttcg tacagccaaa gacgttgagg
     6361 aagttcgtga aatctgttgc gaaactggta acgaccatgt gcaattattt gctaagattg
     6421 aaaaccaaca aggtatcgat aatcttgatg aaatcatcga agctgctgat ggtatcatga
     6481 ttgcacgtgg cgacatgggt atcgaagtac catttgaaat ggttccagtt ttccaaaaaa
     6541 tgattatcac taaagtgaat gcagctggta aagcagttat cacagcaaca aacatgcttg
     6601 aaacaatgac tgaaaaacca cgtgcgacac gttctgaagt atctgacgtc ttcaatgctg
     6661 ttatcgatgg tactgatgca acaatgcttt caggagagtc agctaacggt aaatacccgg
     6721 ttgaatctgt tcgtacaatg gcgactattg atcgtaatgc acaaactctt cttaacgaat
     6781 atggtcgttt agattcatct gcctttccac gtaccaataa aacagatgtt attgcttcag
     6841 ctgttaaaga tgccactcat tcaatggaca tcaaacttgt tgtgactatt actgagacag
     6901 gtaatacagc tcgtgcgatt tctaaattcc gtccagatgc ggacatctta gctgtaacat
     6961 ttgatgaaaa agtacaacgt gctttgatga ttaactgggg tgttattcct gttcttgctg
     7021 aaaaacctgc atctactgat gacatgtttg aagtagcaga gcgtgttgct gtggaggcag
     7081 gcttagttca atctggggat aatattgtta ttgttgctgg tgttccagtt ggtactggtg
     7141 gaacgaatac tatgcgtgtt cgtacagtaa aataaatgtg ttataactat ttacctaaag
     7201 gttagaaggc aagcttctaa ccttttttga tatcaaaaat atcatgttaa ttcactagtg
     7261 ttaatttact ttaatgagtc taagtttgta ttttatacag aatgagaaac gtttattggt
     7321 attgatcaaa aattgtttac tttttttcta gcttagaaca tggtataata agacaataga
     7381 ctaggagtta atatggtaaa gcgagatttt ataagaaata tattattgtt attaattgtg
     7441 attattgggg ctattttgtt gagaatcttt gttttttcaa cttttaaagt ttctccagaa
     7501 acagctaata cttatttaaa gagtggtgat ttagtcacaa tcaaaaaaaa tattcagccc
     7561 aaatataaag attttgtggt ttatagagtt ggaaaaaaag attatgtcag tcgagtcatt
     7621 gctgttgaag gcgatagcgt gacttatatg gacgatattt tttacctcaa taatatggta
     7681 gagtcacagg cttaccttga aaagatgaaa gcacattact tgaatcatgc accgtttggc
     7741 acattgtata cagatgattt tacagttgct accatcacag ctgataagta tcaaaaagtt
     7801 cctaagggga agtatcttct tttgaatgat aaccggaaaa atacgaatga cagtcgtcga
     7861 tttgggttaa taaatgcctc gcagattaaa ggtttagtga cctttagagt tttgcctctc
     7921 agcgattttg gatttgtaga agtagagtag ttgtcaggct actctacttt ttttataatt
     7981 agactatacc aattttatgt ttgacaagac attgtggata ttatatactg taaacatact
     8041 gtttttatcg gtataatatg actataccaa ttattgaaat tatgagagaa agtagcaagt
     8101 taacgaactt gttaaggtaa ttaagatgtg tggaattgtt ggagttgttg gaaatcgcaa
     8161 tgcaacggat attttaatgc aaggccttga aaagcttgaa taccggggtt atgattcagc
     8221 aggaattttt gtggctaatg ccaatcaaac aaacttgatt aaatcagtgg ggcggattgc
     8281 tgatttgcgt gccaagattg gcattgatgt tgctggttca acagggattg gtcacacccg
     8341 ttgggcaacg catggccaat caacagagga taatgcccat cctcacacgt cacaaactgg
     8401 acgttttgta cttgttcata atggtgtgat tgaaaattac cttcacatta aaacagagtt
     8461 cctagctgga catgatttta aggggcagac agatactgag attgcagtac acttgattgg
     8521 aaaatttgtg gaagaagaca agttgtcagt actggaagct tttaaaaaag ctttaagcat
     8581 tattgaaggt tcctacgcct ttgcgttaat ggatagccaa gcaactgata ctatttatgt
     8641 ggctaaaaac aagtctccat tgttgattgg acttggtgaa ggttacaaca tggtttgttc
     8701 agatgccatg gccatgattc gtgaaaccag tgaatttatg gaaattcatg ataaggagct
     8761 agttatttta accaaagata aggtaactgt tacagactac gatggtaaag agctgatacg
     8821 agattcctac actgctgaat tagacttatc tgatattggc aaagggactt atcctttcta
     8881 tatgctgaaa gaaattgatg agcaaccaac cgtaatgcgt caattaattt caacttatgc
     8941 agatgaaact ggtaacgtac aggttgatcc agctatcatt acctctatcc aagaggctga
     9001 ccgtctttat attttagcgg cagggacttc ctaccatgct ggttttgcaa caaaaaatat
     9061 gcttgagcaa ttgacagata caccagttga gttgggagtg gcttctgagt ggggttacca
     9121 catgcctctg cttagcaaga aaccaatgtt tattctacta agccaatcag gagaaaccgc
     9181 agatagtcgt caagttttag taaaggcaaa tgctatgggt attccgagtt tgacagtaac
     9241 taacgttcca ggatcaacct tatcacgtga agcaacatac accatgttga ttcatgctgg
     9301 acctgaaatt gctgttgcgt ctacaaaagc ttacactgca caaattgctg cccttgcctt
     9361 tttggctaag gcagttggtg aggcaaatgg taagcaagaa gctcttgact ttaacttggt
     9421 acatgagttg tcattggttg cccaatctat tgaggcgact ttgtctgaaa aagatctcgt
     9481 ggcagaaaag gttcaagctt tgctagctac tactcgtaat gctttttaca ttgggcgtgg
     9541 caatgattat tacgttgcga tggaagctgc tttgaaatta aaagagattt cttatattca
     9601 atgcgaaggc tttgcggctg gtgaattgaa acatggaacc atttcattga ttgaggagga
     9661 cacgccagta atcgctttaa tatcgtctag tcagttggtt gcctctcata cgcgtggtaa
     9721 tattcaagaa gttgctgccc gtggggctca tgttttaaca gttgtggaag aagggcttga
     9781 ccgtgaggga gatgacatta ttgtcaataa ggttcatcct ttcctagccc cgattgctat
     9841 ggtcattcca actcaactga ttgcttacta cgcttcatta caacgtggac ttgatgttga
     9901 taagccacgt aatttggcta aagctgtaac agtagaataa atgattataa tggataaggc
     9961 aaccatctgt taggtggttg ctttttgttt ttaggagaat agcagagctt agaaaatggc
    10021 cttgagaaaa gctatacaag aagaatagtc atcttctgat gagaacgtat tcatcttgtc
    10081 aaatcattga ttctaaagta aaatggtaac attaagaaat gtttaggagt gtaagatgtc
    10141 attaccaaat tgtttgcaat gccagtctga atatgtttac gaagatggtc ttttattaat
    10201 ctgtccaatg tgtgcttttg aatggacacc aggcgaagaa gcgactgaag aagaaggact
    10261 tgttgtttta gatagcaatg gtgtccgact gtctgatggt gataccatta ctgtggttaa
    10321 agatttgaaa gtaaaaggcg ctccaaaaga cctgaaacaa gggactcgtg ttaaaaatat
    10381 tcgcctggtg gaaggtgatc ataacattga ttgtaagatt gatgggtttg gcgcgatgaa
    10441 attgaaatca gaatttgtca agaaaatcta ataataatac gcaaccagct gatctggttg
    10501 tatttttata aaaaaacctc tccccaaata cgttcaaatt tggtataata gtaaaatatt
    10561 cagttttagg aggcaacatg acctatattc agcaagtttt acccagctta ttagatggtg
    10621 ccttggtgac cttacaagta ttctttattg ttatcattct ttctataccc ttaggggcta
    10681 ttttagcttt cttgatgaag attcccttta aaccgctaca gtggtttttg accctatacg
    10741 tgtggatgat gcgagggaca ccattactac ttcaattgat ttttttctat tatgttttgc
    10801 caagtgtggg gattagtttt gatcgaatgc cagctgctat tttggcgttt actttgaatt
    10861 atgctgccta ctttgctgaa atttttagag gtgggattga ggctattcca aaaggtcaat
    10921 atgaagcagc taaagtatta aagttaaaac ctcttcaaac cattcgttat attattttgc
    10981 ctcaagtgtt taaaattgtg ttaccaagtg ttttcaatga agtcattaat ttggtcaaag
    11041 attcttccct tgtctatgta ctcggtgtag gagatctttt attagcaagt aagacggcag
    11101 ccaataggga tgcaacctta gcccctatgt ttatcgctgg tcttatctat ttgcttttaa
    11161 ttggactggt cacgattatt tcaaaacaag ttgaaaaacg gtttaattat tatcagtaag
    11221 gaggcttcat gttagaactc aaaaatattt ccaaacagtt tggtcaaaaa accatttttg
    11281 atggctttaa tctaaccgtt caagacggag aagtgctctc tttggtaggc ccatcaggtg
    11341 gcggtaagac aacactctta cggatgttgg ctggtcttga atctattgat tcaggtcaag
    11401 tcttttacaa tggcgaagat gttggtattg atcatttgga aaatcgtaac ttgcttggat
    11461 ttgtttttca agattttcaa ttgtttcctc atcttaccgt tttagacaac ctaacgctgt
    11521 ctccaacaat tacaatgggc aagcaaaaat ctgacgctaa agaaaaggct ttggatttac
    11581 tggcacgttt aggattaaaa gaacatgccc aagtttatcc ctattcgtta tctggaggtc
    11641 aaaaacaacg ggttgctttg gcaagagcca tgatgattga ccctcaaatt atcggttacg
    11701 atgaaccgac cagtgctcta gatcctgaat tacgtcagac ggtagaagcc ttgattgttc
    11761 aaaaccgtga aatgggaata actcaaattg tagtcactca cgatcttgtt tttgccgaag
    11821 ccatctcaga tcgtattatt agggttaatc ctaaatagaa gaggacccaa aagatgatta
    11881 taaaaaaaag aaccgtagca attttagcca tagctagtag ctttttcttg gtagcttgtc
    11941 aagctactaa aggtcttaaa tcaggagatg cttggggagt ttaccaaaag caaaaaagta
    12001 ttacagttgg ttttgacaat acgtttgttc ctatgggcta taaggatgaa agcggcaaat
    12061 gcaaaggttt tgatattgat ttggctaaag aagtttttca ccaatatgga ctcaaggtta
    12121 actttcaagc tattaattgg gacatgaaag aagcagaact aaacaatggt aaaattgatg
    12181 taatctggaa tggttattca ataactaagg agcgtcagga aaaggttgcc tttactgatt
    12241 cttacatgag aaatgaacaa attattgttg tcaaaaaaag atctgatatt aaaacaatat
    12301 cagatatgaa acataaagtg ttaggagcac aatcagcttc atcaggttat gactccttgt
    12361 taagaactcc taaactgctg aaagatttta ttaaaaataa agacgctaat caatatgaaa
    12421 cctttacaca agcttttatt gatttaaaat cagatcgtat cgatggaata ttgattgaca
    12481 aagtatatgc caattactat ttagcaaaag aagggcaatt ggagaattat cggatgatcc
    12541 caacgacctt tgaaaatgaa gcattttcgg ttggacttag aaaagaagac aaaacgttgc
    12601 aagcaaaaat taatcgtgct ttcagggtgc tttatcaaaa tggcaaattt caagctattt
    12661 ctgagaaatg gtttggagat gatgttgcca ctgccaatat taaatcttaa aaaggacact
    12721 tgtccttttt ttgtttaaca ggtgagatct agcagacaaa gtcagctaga tgatttaata
    12781 aaaatcttag tttaaggttt tttattaaac tgtatttttt ctaaaaatta tagctttttt
    12841 agactgataa atgacaagaa aagttatttt aggtcaatta acgtccgttt aaaagtaata
    12901 ctagacattt aatgacatta aaaaatgatt atttttgcga aatcctctta ttttctggaa
    12961 attaaagaaa tattgacctt agtaaaaaca agtagtatga taacgttata agcgcttata
    13021 aattaattta acttcactta actatttaaa taggtttatt atattcagga ggaataatat
    13081 gaaacccaaa caccttctct gtctttcaac agttgttgct ggattagctt tattttcaac
    13141 tatgacgcac tcagttttag ctgatgatgc ttcaaatccc gatactattt taatgaataa
    13201 caaccaagcg aatcttcaaa gagacgctct agtgcaaaag cttgatgagg gacatcaaca
    13261 attagaagct attaaacatg aagctaaagg tactgatatt gaggctactg ttaacaaagc
    13321 tatcgatgct gttgatcaca tgaagagttc tatacgcttc aatactgaaa cgatctatga
    13381 ttttagttca attggggcaa gagtagaagc attatcagat gctatcaaag caatcgtatt
    13441 ttccacaacc caattaactc acaaagtaga aaaagcgcat actgatatgg ggtttgctat
    13501 tactaaatta gtcattcgta ttatagatcc atttgcatct gttgacgcta tcaaagctca
    13561 agtacaagaa attaaagcac ttgaggaaaa agttatcaat tatcctgatt tacagccaac
    13621 agaccgtgct actatctata ctaaagctaa actaaataaa gctatttgga acactcgttt
    13681 agaaagagat aaaaaggttt tagggatcaa atcatttgac gtttataata gacttaacaa
    13741 agctatcaca catgcggttg gtgtccaact aaatccgaca accactgttc aacaagttga
    13801 cgatgaagtt attgctgtgc aaaatgcttt ggaaacagct cttaagtcat agagtgtata
    13861 agtaatgtgt tgtataggta gatgattata ttaggatata gaataggtaa agattttaaa
    13921 tatcttacca ttgtagagtt tgaggtgatg ttctcataaa caatgagtca cctgtagccc
    13981 tttttgcctt taacatgatg taccaattta aggtattgtt caaccgtcac tattgaatct
    14041 tgtttaacct cctaaactaa taagattcaa tatgaaaacg tgtattctta aaaaaatccc
    14101 tatgatctgt ttaaaaacaa attgtaggga tttttttaac tatttaactt attattttgg
    14161 gactaggaat cgctttagga atatcatgaa gtatagttat gttataagat aagtaatgtt
    14221 tttacatttt tacaatacga tagtgtgtgt gatgtaattg tctcttagcg ataggggtaa
    14281 tttggtggaa gagtaaggac caagattcat gacgcctcca aatactggta tgttgattgc
    14341 catttaattg gtaagttacc aacatcgttt tttgactaat ggattcaatc tgaaaatggt
    14401 tgaagttact tggataataa atttcttggt tcaaaaattg ttcatagtta actgtttggc
    14461 cattcgcatc aaccagttgg aaagacttag caatgtagtg gtcatagtca ggaaccttac
    14521 tcaaaattaa tttttcgtag cggtagagct ttttggcgac attatcaaaa atctcttctt
    14581 cgagaatcat ggcaggttca acgtgaatat caatgtcata aatagaaaat tggtcactca
    14641 ataactgctc cactttctct gtaatagagt gactttcata gactgaaaga tcaggattca
    14701 tttcaagtac aatatcaaga tagacattgc taccataggt cctagctcgt tgagacttaa
    14761 cggcgacaat tttagggatt tctaaaatgg ctttttcgta ttttttcaaa tgacggctat
    14821 caaatccatc agataaacta aacgaacttt ccataaagat atcaaaagct gttttaagaa
    14881 taaagaacgt aatgatcata gcagctatat gatcgataac tggtaaatgc aaagaagctg
    14941 ctataatagc aattgatgtt ccgatagaag taacagcatc agctagatta tccttagaag
    15001 cagcgactaa ggcactggat tttacacgtt tgga
//
LOCUS       DAUVUY010000043        14976 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.043, whole genome shotgun sequence.
ACCESSION   DAUVUY010000043 DAUVUY010000000
VERSION     DAUVUY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.043"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            432..941
                     /locus_tag="VOX58_001121"
     CDS             432..941
                     /locus_tag="VOX58_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HES5878353.1"
                     /translation="MSTDHHLREVSYQLLAERGVSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGNSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            complement(1023..2111)
                     /locus_tag="VOX58_001122"
     CDS             complement(1023..2111)
                     /locus_tag="VOX58_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HES5878354.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGNSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(2168..2836)
                     /locus_tag="VOX58_001123"
     CDS             complement(2168..2836)
                     /locus_tag="VOX58_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HES5878355.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYEGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAINRENA
                     DSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            complement(2849..5266)
                     /locus_tag="VOX58_001124"
     CDS             complement(2849..5266)
                     /locus_tag="VOX58_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HES5878356.1"
                     /translation="MIETKSPYFGHLTDRMAHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGRLTPEELWHALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            complement(5477..6781)
                     /locus_tag="VOX58_001125"
     CDS             complement(5477..6781)
                     /locus_tag="VOX58_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HES5878357.1"
                     /translation="MAKMNMQNIIKPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSDVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGIIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            complement(6791..7099)
                     /locus_tag="VOX58_001126"
     CDS             complement(6791..7099)
                     /locus_tag="VOX58_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HES5878358.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDKSEAICKDNDIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            complement(7127..7447)
                     /locus_tag="VOX58_001127"
     CDS             complement(7127..7447)
                     /locus_tag="VOX58_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HES5878359.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            complement(7735..8715)
                     /locus_tag="VOX58_001128"
     CDS             complement(7735..8715)
                     /locus_tag="VOX58_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HES5878360.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRKQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            complement(8731..9477)
                     /locus_tag="VOX58_001129"
     CDS             complement(8731..9477)
                     /locus_tag="VOX58_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HES5878361.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLEVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKIITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSSGGLVLDFVS"
     gene            9603..10376
                     /locus_tag="VOX58_001130"
     CDS             9603..10376
                     /locus_tag="VOX58_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HES5878362.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            complement(10613..10789)
                     /gene="secE"
                     /locus_tag="VOX58_001131"
     CDS             complement(10613..10789)
                     /gene="secE"
                     /locus_tag="VOX58_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HES5878363.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(10803..10955)
                     /gene="rpmG"
                     /locus_tag="VOX58_001132"
     CDS             complement(10803..10955)
                     /gene="rpmG"
                     /locus_tag="VOX58_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HES5878364.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            complement(11004..13340)
                     /locus_tag="VOX58_001133"
     CDS             complement(11004..13340)
                     /locus_tag="VOX58_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HES5878365.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            complement(13379..13759)
                     /locus_tag="VOX58_001134"
     CDS             complement(13379..13759)
                     /locus_tag="VOX58_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HES5878366.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            14336..>14976
                     /locus_tag="VOX58_001135"
     CDS             14336..>14976
                     /locus_tag="VOX58_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HES5878367.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVT"
BASE COUNT         4225 a         3141 c         2953 g         4657 t
ORIGIN      
        1 gctaccattg aaaatttcac tagccgacat tttttagaaa aaacccgtct tgatccaagt
       61 agattcatat gcttgtcctc ttttctaatt tcgctcacta accatagtat acgcttactt
      121 aactagttta tcaatagttt ttttaaaaaa tatgaaaatt aaacgagata ataaggagaa
      181 aaaagaaggc gttgtctttt tgttttgcca tactaatatc atttttgaaa taggtgctgt
      241 gtgtcaccta ggggtggtga ttgtatttag gcctaggtaa gatggttact ttactatcgt
      301 ttctttgact tttaccaagt agggggtctc agtagtcaac atctcttaaa aaagaactat
      361 cccctcttta gctgttgtag aggtttctta aaatctttta tgttacaata aagaaaaaaa
      421 ggaggtctca catgtcaact gaccatcact tacgagaagt ttcttatcaa ttactggctg
      481 aacgaggggt ttctttagaa gctattgcag agctggtgct ttttttgcag aatgattata
      541 ttcccaattt gaccatggca gaatgtttag agagcgttga agcggtgctt gcaaaacgag
      601 aagtgcaaaa tgctattatc actggggtag aattggataa attggctgaa gccaaccagc
      661 tgtctgagcc actccttagt atcttgaaaa ccgaccaagg gctgtatggg attgatgaaa
      721 tcctggcctt atccattgtt aacctctatg ggtctatcgg ttttaccaat tatgggtatc
      781 tggacaagac aaaaccgggg attgttgaca aactcaacca caaagatggc aactcctgcc
      841 ataccttttt agatgatatt gttagcgcca ttgcggctgc agctgccagc cgtattgccc
      901 acaatgaccc tgctaaaagt gctattacca accaacaata agtaaaacaa gtctaggaaa
      961 tgactcccta gacttgtttt tttgaggtta tctggcaaat aagaaatctc tttttggtta
     1021 gcctatcgac tcgtaacata ggcatcaaca gcaaccaaag ccgctgctac atcttctgga
     1081 ctgataacaa atggcatttg atggatggtt tcgcctgcca tggttgcttg acggccaatt
     1141 tttaagaaat cctcttgagt agccgtgtct aaatgcattt ctttgagggt tgttggcatg
     1201 ccaatagctt ggtaaaagtc gatgtagcgg tcaatttctt cacgcgaacg attttccaag
     1261 aaaagttggg tcaacgtgcc ataggcgact ttttcaccat gggtaaggtg atgaatagca
     1321 cctgtcagcg ctgtaaagcc attgtgaatg gcatgtgcag cagccagacc cgcactttca
     1381 aaaccaagcc cactaagaag tgtattggct tcaatgacat tttccaaggc cggggtcacg
     1441 acttgtctgt cacagctagc catcgctttt aggccatctg caaacaaggt ctgctcacag
     1501 gctttggcaa tggcaactcc tgccaaggtt tgattgccac ctgccatggt gtctccattt
     1561 ttttgcataa cagcacgcgc ctctacccat gttgctaacc catctgcaat ccctgacgcc
     1621 aataagcgtt ttggtgcctg acaaattact tgtgtgtcaa ccaaaacaag atctggattt
     1681 tttgaataga aaatgtattt ttcaaaggct ccttcatcgg tataaataac cgataaggct
     1741 gaggttgggg catctgttga agcaatggtt ggggcaataa tcaccggaac agcaagtaag
     1801 tcagcgatag ccttagcgct gtcaatggtt tttccaccac caagtccaat aataacatca
     1861 ttgccatttt ccttggcaat ggcaaccacg cggctgattt cattatctga ggcttcgcca
     1921 ttaaaggcta cgtgaacagg agtcatgcca ttatctatca aatagctttc aaatctttct
     1981 ccgacgatgc catacaccac gtcatcacac agtaaaatgg ggctatttcc tagttgtttt
     2041 agggttttag cattggtaaa caaggcattt ttaccttgaa tgtaacgaga tggacttgca
     2101 aatactttca tcgttgaact cctttatctg tgattcctaa tgatgtgatt actactgatg
     2161 ctatttctta aatgctcttg cggtgatgaa tggcttccca atccttgcca aaatcgtcaa
     2221 ccgctttttg aatagatggc atggcaaatc ctgcttcaaa cacatctggg ccagcagtga
     2281 tggcttgtgc tcccagagca aaggatttat tgacctgagc cacattttta aagcttgctg
     2341 ccaaaatctt actatcagca ttctcgcggt taatagcttc tgctaattgt cctatgacag
     2401 cttctggatc aatattgaga ttttccatac ggttatagta aggagccaaa taatcagcac
     2461 cagcttcaat ggctaatagt ccttggaagg tcgtataaat agcggttgct gtgatgtgat
     2521 agccttcggc tttcaaggtt ttaatggctg caaggccttc tgtggttaca ggtactttga
     2581 catacacact atctccacat tgcctgcgga tttcagcagc atcttttaag atgccttcgt
     2641 aatcttgggc gatcacttga acatgaatag aagccttgtc accgataatg gcacgaactt
     2701 ctcgaatccg ttcaaagaaa tcaatgtcac cttctttttt ggcaatagaa gggtttgagg
     2761 tcacacctgc gagaggcaaa atgtgatgcc attttttaat agcttctaaa tctagagtgt
     2821 ctaacatgta ttccatactt attttctcct aaagtgtgtg ttctgtacgt tcaataatgt
     2881 catcttgggt tgcttttgag agcacattga agaaagctga gtagccggca acacggacaa
     2941 tgaggtcacg gtgcttttca ggatgttttt gggcatcaat caaggtttca cgtgacacga
     3001 cattgtactg gatatgataa ccgtgtaggc gattaaagaa ggtacgaagt aaggccatta
     3061 atttcagttt gtcttcttct ttggccaggg tttgtgggtt aactttttgg ttgagtagca
     3121 ctcctccgac aatctcatct gttggtaatt tagcaacaga tttcaatact gaagtcgggc
     3181 cttttttgtc catgctgtgt tctggtgaac agccttctgc tagtggtgtg cctgcatggc
     3241 gaccgtctgg tgtcgccaag gttccttttc cttgtccgac attggctgag attgatgatg
     3301 tcccagaata gcggatacct ccgattgggc cacggccata ccgggtattt gggtatttgg
     3361 caatttcatc aatgtaggtg tcgtaagctt cgacaactaa actatctgcg tagtcatcat
     3421 cattaccata ttttggtgcg tcattgatga gcatttgtcg gatctcttct ccgcgctctc
     3481 cagcaaagtc actttcaagg gcgtgccaga gttcttctgg agttaatcga ccttcttcaa
     3541 atactaattt tttcagcgct gctagggagt ctgataggtt ggcaataccg acttgaaggc
     3601 ctgagatata gtcgtaaaca gctccgcctt cttttaaggt cttgccacga ccgatacaat
     3661 catctgtcaa ggctgagcaa agaatatctg gtacttcacg ttcaagacct aggtcgatgg
     3721 cattttcaac aatgacgctc atgcgggtaa tttcgcgcag ggtggcatcc catgctgctt
     3781 ttaattcttc ataagatgtc atgtctttga agtgaccgtg tccttttgca aagcgcttgc
     3841 ctgatgctgg gtcaatcccg tcattcatgg taatcagcaa aattttaggg aagttgatgt
     3901 aactcatccc cgtgcaacgg tagccccatt tcccaggaac tgctgtttcc acacagccaa
     3961 tggctgagta atcataggcg tcttcttctg agacaccttt tttgatgaaa gatggaatga
     4021 tgatttcatc gttgttcatg gctggcatac caaaaccaag tttcatgacc tcgatacact
     4081 cgttcataaa ggtattgtct aggcctttat ggtaacgaac agtcaagtta ggttgtggca
     4141 atttggtttg agccacactc cttagaacaa ggtaagacag tgggttgacc gcatcttttt
     4201 tatcgcgtgt ttgaccgccg atcgtcacgt tttggtaaag tggactccct gctgatgaga
     4261 aggtgtgggc ttggctccgt actttattaa tggtcaaggt cttaatccag aggttggtca
     4321 aacgctcaac aattgtgtct tctgtttcac ggccagcctc caaatcagct ttgacataag
     4381 gatacatgta ctggtcaaag cggccataag atagggaatg cccatttgat tcaatttgca
     4441 aaatacactg aatgaaccag acagattgca cagcctctgc aaaggttttc gctggttcgt
     4501 aaggcacttt atcacaaatg cgcgcgatgt ctaacaattc tgcttgacgt tctggtttag
     4561 ccgtttttgc tagttctcta gccaacttgg catagcgttt ggcataagtt ttgactgcat
     4621 caatgacaat aaaaacagag tcgtaaaaat ggtatttgtc aatgttttca ggaatggtca
     4681 aatcaagcgc agctttggca gctcgtgccc gttcttcaaa tccctttaag ccgtgctcca
     4741 agagtttttg gtaattaacc gctaaatggg catccccaga gttcatcttg ccttccatgc
     4801 caaagaaacc tgtttccatg tacacttgca cttcttctgg tagcaagacc ccgcagcgag
     4861 cacgtaagtt attgttttcc cagaatgggg caatgtctcg gagctgttgc ttggtttctt
     4921 cggtaatgta gaagacatcc ccatctcgtt tttcaaaaag atcaagttcg ttgaggacaa
     4981 attccagagt gtattctggg aaaatcggcg catccttgtt cgaagaggct tggttaccag
     5041 caatgaggct ttcatcctca atatagatgg tcatgttttc caaaatagtt tgaagcatgt
     5101 aagcgcgttt gaggtttgct ggcttatttt ggtgtttttg ataggcttca gtggctaaaa
     5161 tagcacgctc agcatcaata taaggtttct tgtctaacac agcttcacga tagtgagcca
     5221 ttctgtctgt taaatgtcca aaataagggc tttttgtttc aatcatgcgg ggtctccttc
     5281 tttcttacga aattataaat gtttctttcg taactatttt atcatatatt gcgacttttg
     5341 cgtatcctta accataagaa gagatagtca ctttattttg cactattgtg caatataggc
     5401 ctgataaatc gttcttcttt ttctaaaaaa ccacctctaa ctggagatgg tctcaagaat
     5461 ttgtgtggac gctaaatcat gactcttcac tagcttgttc attttgataa gccatattat
     5521 cttggatttt aaagaatggg aagtacacca acgttgacat gatgagtata agaatttgca
     5581 caatagctcc ttgccagcca ccaaccatga accctgagat aatggctggt gttgaccacg
     5641 gaagggtcac tcctgcaaag ggctgcatga aaccaatagc aatggcgcca taaactgtga
     5701 gagctgccaa gacaggaacc aagataaatg gcaagaacat gactggattc atcacaattg
     5761 gaaaaccaaa gacaactggt tcattaacat taaagagggc tgggaaggct gcaaccttac
     5821 ctaaagcctt gtattgtttg gatttagccg caaagatcat agctaccact aaaccaaagg
     5881 taatgccaga acctgataga atcaagaaag agtctaaaaa ttgttgggtt acaatatggg
     5941 cacccttgtc tagggataac tcacctgctg ccatcaaggc cttattggca tctagatttg
     6001 ataagagaag agcagtgaca attccattga ccacagattg cccatgcacg ccaaaccacc
     6061 aaagaaagga aataaagaag gcaataccta gtgcgccata aagagaaccc gttaaccctt
     6121 gcagcggtac ctgaatgaca tcataaatca tttcaataaa ggtgccacca cctgttactg
     6181 acttggcaat aatatacaca agcattgaca aggtaaagat cacaaaggct ggaatcatgg
     6241 cttcaaactg tttggcgatg gcttgtggca cttgatctgg cattttaatg acgatatgac
     6301 gacggataaa tgttgtgtaa acagctccga ccgttaaacc aatcacaata gcaccaataa
     6361 tcccttgacc accaaaccaa accttactga tagcatctcc aatagcttca ccttctgctg
     6421 gaacataaga tgatctcaat aaaataaaga aggctgacaa ggatagaacc cctgaaggga
     6481 gaggttcaac atctgagttt ttagcataag aatagccaat tgcaaaacag gaaatcaacc
     6541 ccataatagc aaatgttcca tggtaaacct gcataaaggg ttctgtccag tcagcaccaa
     6601 acacactagc aatggcatca ttaacccctt ggaaaggaat ctgcccagca atcaggaaaa
     6661 gactccccac aacggtcaaa ggtaaaatgg ctaacatacc gtcttttaag gcaatgatgc
     6721 cgcgcatatt cacaaatgtc atgattggct tgatgatgtt ttgcatattc atttttgcca
     6781 tgattgactc ctattcttta acaaggctaa gggctaaatc aagcaccttg ttgccgtcta
     6841 acattccata atcagccatt gggataactg caatagggat atcattgtct ttacaaatag
     6901 cttctgattt atccagagta taggcaacct gtgggcctaa aagtgccaca tcaagtaatg
     6961 gtgcatagtc cgctaatttc ccttgagcat aggcttcaat ctgacagtca atgccttttt
     7021 tttcagcagc tactttcatg ttgttcacca acatgccggt tgaaaatcct gctgcacaaa
     7081 acaatccaat tttaatcatg tcaatctcct ttttagtcat gaaaacttat ttagttgcca
     7141 attctttacg aaggtcaata atctctttga tgagattaat ttctgtaatg gtcgtcatca
     7201 agtgatcctg tgagtgaaca aaaagagctg taatctctga cttattacca ctagcttctt
     7261 gggcaagaaa ctgggtctgt aaattgtggg cttctaataa ggcatcatct gctaaggcca
     7321 tttctttttc actagtggca taatcgtcct ctttagcaca tttcagagct tggtagatat
     7381 gttgtttggc atcgcctgca tttaaaatta agcccataat gatttggtct ggtacaataa
     7441 cttgcattgg tgtctccttt ttttcttttt catgtgatgt atcgacaatg agtctttttt
     7501 ttcgaaggtg atgttattat gtttgtaatc gtttacattt cgttggtaag tttattgtat
     7561 catttttgtt tttagggtca atatattttg tgctatattt tgtttaatgc acttttgtgc
     7621 caatacatta aatgcacatt tgagcaatct ctgttttatc aacacatcaa acaaaaaagc
     7681 cctgaccgtt tggtcttggg cctctataaa tatcatagta tctttgaatt tctattaaag
     7741 aagaacgtta ttaagatcaa tatcttcatc taacgctaaa acagctagca tggtctcttt
     7801 atccgtcact agataattga taaaacctgc ttttaaggct gccaatattg ccttagcctt
     7861 gtgtttacct gttgcaacag ccattgtttt agggacacgc ctcaactgtt ctagcgaaat
     7921 accaatagtt cgatcctgta ggccagtgta aactggctga cctgcctgat caaagaaacg
     7981 acaacaaact tcaccaacgg ctttttcata tttgagttgg tcatgatctg agctggttaa
     8041 taaatcacgc cattgactct gctcaaggct attaggctcc ccaccaattc ctactaaggc
     8101 aagatctaac tggtcccagc ttgttaaaat atcgttaaag tatttagatt gtaagattcc
     8161 cttagccaag tgtttgtctt cttgaatgac catagcgttc ataaaagcgc tattaccatg
     8221 aaagatgcga gccaagcggt aaaccagggt attgacatgg tatttggcat taatatggct
     8281 aggtcctcct gccaaaggat aaataaacac atctttcatc gcttttggat ttaactcatc
     8341 catcaagcaa gaaagagtcg ctccccagga cagaccgatc ttatcgccat ctttaacaac
     8401 atgtctaaaa acctcagctg ctgttttagc aacatttgtc aaggtatcca ttggggtatc
     8461 ttcaacctgg ttgggcacaa ggtctagttt ttccaagccg tactgctttc gcacgtattc
     8521 ttctaaagca aataaatcag catcatagtc ggcaatttca atccgaacaa tgccttcttc
     8581 cttggctttt gctaacatcc gacaaaccgt tgttcggtag atattcattt cctttgaaat
     8641 caaggtttgg ctcttgcctt gaacataatg caagtaagcc accttggcaa ggagccgtct
     8701 gcgttcttct ttcatctagc cacctatttt ctaagaaaca aaatcaagaa caagaccacc
     8761 agaacttaat aactcttgtg tttgcttgtc tagtgcttgg tctgtaatga cgcgattgac
     8821 atcagctaat gacaactggt gtaccagact ggtttttgaa aacttgctgg aatcggtcaa
     8881 aatcaccact tcctcagcag catctgccat gtaacgcacc ccttctgagc gcatcatgtc
     8941 cttgcccata aagccaagat ctttgttgaa accgtctgtt ccaacaaaga cacgattgac
     9001 atgaaaaagg gatatcattt ctttcaataa aggaccaaca gtcacctctg aattgggctg
     9061 gtaataaccg cccagtaaaa tgatttgaca gctactatat tgacgaatgt agttggcaat
     9121 gaagcacgaa ttggtaatga ttttaatatt tcgtttggtc tggcaaagca cttctgccaa
     9181 aagggcacag gttgagccag attcaatcat aatggtatcg ttgtcttgca ccaattcagc
     9241 tgctttttct gcaatccgtc gctttatgtt gtaattgtag gacaagcgta cattgaggtc
     9301 atcgccactg tttagcaccg catagccgtg ttcccgtcgc aacaagccct tactttcaag
     9361 tttatcaagg tcttttcgaa tggttacttt cgaaacttct aattgctctg ataaactgtt
     9421 aacatctatt tttttcttct gggaaaccag ttgaataata cgttctaatc gattcatcat
     9481 tcacccccct ttcattgtac cagttatttt cttaaaagaa aagagatatt tactttcatt
     9541 cgtaattgat ttatttacgt ttatctggta tgatggtttt acaacgaaag gaattcacac
     9601 ttatgactga tcgcggtatt gttttcaaca tccaacattt tagtattcat gacggccctg
     9661 gtattcggac aaccgttttt ttaaaaggtt gtcccttgag atgtccttgg tgtgccaatc
     9721 ccgaatccca acaaaaagca cctgagcaga tgttaacaag tgatgggctt aatactaaaa
     9781 tagttggaga ggaaaaaacc gtggacgagg tgatcgaaga agtccttaaa gaccttgatt
     9841 tctacgaaga gtcaggtggg ggcatgaccc tgtcaggggg tgaaatcttt gcccaatttg
     9901 actttgcctt agccctgtta aaagcagcca aagcagcagg tcttcacacc gcaattgaaa
     9961 ccaccgcctt tgccaaacac gagcaatttg tcacccttat tgactacgta gactttatct
    10021 ataccgattt gaaacattat aatcagctca gacaccaaaa agtgactggg gttcgtaatg
    10081 acttgattat caaaaatatt cattatgcct ttcaagctgg aaaagaaatc gtcttgcgca
    10141 tccctgttat tccccaattt aatgattcac tagacgatgc taaagccttc tcagagctct
    10201 ttaatcagct tgagattgac caagtgcaac tgttaccttt ccatcaattt ggagaaaaca
    10261 agtacaaact tctaggtagg gagtatgaga tggctgaggt caaggcttat catcccgaag
    10321 atttagcaga ctatcaggct gttttcctca accataatat tcattgctat ttttaatgtg
    10381 ctaagcaagt gaggccaggc tgtttatgcg ttgactctgt tacaagctaa tttagtgaac
    10441 ttggtttgga gatcgttgct acattgcctt tacctttatg cttttgaata gctcaaacca
    10501 gtatgggctt taaaaaagct tactaattag attattgata ctacaaaaaa ggctgggaat
    10561 actcccagcc taggcttttc caagcaagct tatcatttct accaatcttg acctaaaata
    10621 aattaatcag tgctaataca ctctttgcta agagctggtc aaagatgtag ataatcaccg
    10681 taaagaaagc tgtgtactca agaactgaga taaaatcttt ccaacgttgt ttacgatttg
    10741 gccatgttgt gtcttttaag actttaaaag tcccgctaat gaatcccatc aggctctcct
    10801 ttttaacgtg tttctttgtg taaggtatat gttttgcagt atttgcaaaa tttatttact
    10861 tctagacgtg ttggtttagg cgtgctactc actccgatag agtagttacg actgccacat
    10921 tcgacacacg ctagactcgc ttttttctgt gccataacgc ctccgtaact attattttaa
    10981 cacgatctct aaaatactgc aagctatcta aaatagtcaa caatctcttt ccaaatagat
    11041 tgggctttat cacgtaaccc tgactgatcg actgcttttg atagtgattg cgaagcttct
    11101 tgtgctgatt ttcttaaacc atcaataata gattgagtgt caactcctga ttgattacct
    11161 gtttcattga ctccataaac agctgaaata ccattttgag cgtaggcatt atctacatga
    11221 aattggctgc ccttggtata aggcaaaatg taagatgcct gagtgctaaa aatagctgaa
    11281 gccgtgcctg cacttgaatc cgttagataa tgattttcat cggtctgatt aaatcctacc
    11341 cattgactaa taacaacatc tggcgtataa ccaataaccc actgatcgcc tgctaagtcg
    11401 ggattgaagt tggtttctgt cgttcctgtt ttaccagcta gtgtatagcc atatacgtta
    11461 gcattgactg ctgttccatt tgaaaaggta cctagcatca tggctgtcat cttatctgca
    11521 acagactggc tgacaacacg tttagcctta tcagtaaagg ttttaagcac ttcacccctg
    11581 gcattttcaa tccggttaat caagtgcgca ggatggatta ctccattatt ggcaaaggca
    11641 gcatatgcct gagccatctc caatggattg gttgtcacgc tgccacctaa agctaccccc
    11701 aactcttttt tggcagagct catatctaac ccaaatgttt taccataggt aaaggcttta
    11761 tcgattccga tatcgttcaa tgtagaaact gctggaatat tataagagtt tgctaatgct
    11821 tgatacatag gaacatcttc tgattcataa tttccataat tatgtggctg atagccatca
    11881 aaatcttgaa cggtatttgg tagttctttt tcaattgacc atcctgaagc aacggctggc
    11941 gcgtaaacca cgagtggttt gattgttgat gcgggactac gttttgcttg agtcgcatag
    12001 ttaaaacttc tgaaagtcgg attttcacta ctattcacac gaccaatcag acctctaaca
    12061 cctcctgttt taggatctaa agcaacacta gctgcttgag cagagctacc gtcataagct
    12121 gaaacaggaa atagttcatc gttgttaaaa gtcgtctgca tgccagtttg gtaattttga
    12181 tctagctcgg tgtaaacttt gtatccatta ttaacaatgt ctttttctga aagaccataa
    12241 gttgctattg cttcactaat aacagcatca aagtaggatg ggtatttgta gtcatctgtc
    12301 ttaccaacat aagtatcagc taagcgattt tttagcccta ctgctctagc ttgttgagct
    12361 tttgtttggg taatcttttt ggcatcaacc atcgctccta aaacagtatc tctacggtga
    12421 gtagcatttt ttagagaatg gtaagggtta tatatttcag gtcctttgag catacctgct
    12481 aatgtggcag cttcatccag tgttaagtta gcagctgtgg ttccaaaata tttttgactg
    12541 gcatcttcaa ctccccaaac tccatttcca aagtaggagt tgttaaggta catagtaaga
    12601 atatcttttt tactgtattt tttggtcaac tctaacgcca aaaaaaactc tcgggccttt
    12661 cgtttaattg tctgatcttg tgagagataa gcatttttag ccagttgctg tgtaatcgtt
    12721 gagccacctc caaagcggcc cgccgtaact accgccaata agaagcgttt aagattaata
    12781 ccgctattac tgtaaaaagt cctatcctca gtggcaataa cagcattctc aagatcatct
    12841 gaaatagcgt tgagctcaac ataactccct ttttgaccag ataaactgcc tgcatactct
    12901 cctttgtgat cataaataac agttgtagcc ttcaaggcat cttgtaaatc agatacttta
    12961 gctgtttttg ataagtaaaa caagtatgat cctaagagta agacaagagt tccaatcaga
    13021 atcattagca gttttccgat gtgatagcgg cgccaaaaac gccgaattgg atgttgagga
    13081 gacggcaaaa cagcttccaa cttttgcaac caagcgggcc gacttttagg gcttccttca
    13141 taggctctac tgcgttgcag agctattgca gggtcttgtt ctgagttttt ggtagctgat
    13201 gagtccaaat gattggcatt tggcgaatac tgcttttgat tgtgttgtgg cgttaaacgc
    13261 gttttttgat gcattaagaa ttgtttttcc tgatatgctt taggaaaaaa tttcttttgt
    13321 aaaagttcta gaaatctcat aacgttatta taccactatg atcttaaagt aaccttgatt
    13381 aggtcatcga aatatgtttt gaataagcca caccatccac ttgaaagagt agtccttgag
    13441 gtccaccgtg atgtgcaaat tccgtttgaa tcgattttcg agcaggatat ctgccattaa
    13501 agcgctcttt aatcattttt tccatcacag gtatgttcca aacgtctcga aatagacaat
    13561 ccatttgaac aactgcatct aatgtgagcc ccaccaatgc taatcggcgc tccatctcgt
    13621 caaaggctcc attaatctga ctttcaatgt cttgtccaac attgccgaca caatagttta
    13681 aataatagaa atctcctgct tctacaaggc ctgtatgccc tctatcttcg ttaacatcat
    13741 aacgtctaat tgttttcatt gtttttcctc tattttacga ttatgttttt atcataatta
    13801 taacaatttt tcttaatatt ttatattaaa aacctttggt tgacttcttt tacgatagag
    13861 aaggacctat cataaggcca aactaatccg tatttttccg atacgtcgtt caacctcatg
    13921 gggttgtttt gaaagaacat acctgtttga ctcatcgtct taggtaactg tgtgttgata
    13981 agttttatct tgctattttt ttatgactgt tccaacttcg caacacgtta gttgttttct
    14041 atttctgcgt tagcacctag tttcatatca tttctgttac aaactctatc aagaggtctt
    14101 atttaagaca ctttcttatt agatggatta gcaaaataac gtttttacct ttttgagtgg
    14161 cacgttttga ttagaaacat gctataatag cattgaattc cttgcattaa tcaggagctc
    14221 acattaaaca tagaaggacg ggtttaacca atgaataaat tccaagcttt taaagaaacc
    14281 ttatccgcag aaagtctaaa agccatttac gatgagactc gcttggaggt agctaatgac
    14341 gagcgtgaag gtactgaagc tttctctgct gctcttgcca cccaaatggc tatcaacctt
    14401 gttgaaaaat accataactg gctcaatgaa gacaacaaat aaaccatcaa aggggtttac
    14461 tgtcacactg aaaaatccct ttgaagaatt gacggtcaaa gcattattag aggaacaatt
    14521 gttaattccc cgtaaaatta ggcattttct gagaactaaa aaacatgttc ttattaatgg
    14581 tcatcctgtt aactggcaaa gttgtgttaa atacggtgat caggtcaaac tttttttcga
    14641 tcacgaagat tatcctgaaa agattatcgt gatggggcag gccgaaaaag taacctgcct
    14701 ttatgaagat gagcacatca ttattgttaa caaacctgaa gggatgaaaa cacacggcaa
    14761 tgatccaaca gagttggcgc tgctcaacca cgtgtctgct tacacgggac agacctgcta
    14821 tgtggttcat cgtctggata aggaaacaag tggggccatt ttatttgcta aaacaccttt
    14881 tatcttacca atccttaatc gtcttttaga aaaaagagac atccatcgag aatatttggc
    14941 actagttcac ggatccttag acagtcctag ggtaac
//
LOCUS       DAUVUY010000044        14911 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.044, whole genome shotgun sequence.
ACCESSION   DAUVUY010000044 DAUVUY010000000
VERSION     DAUVUY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.044"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            79..333
                     /locus_tag="VOX58_001136"
     CDS             79..333
                     /locus_tag="VOX58_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HES5878368.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            354..749
                     /locus_tag="VOX58_001137"
     CDS             354..749
                     /locus_tag="VOX58_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HES5878369.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            1035..1354
                     /locus_tag="VOX58_001138"
                     /pseudo
     CDS             1035..1354
                     /locus_tag="VOX58_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1351..1914
                     /locus_tag="VOX58_001139"
     CDS             1351..1914
                     /locus_tag="VOX58_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HES5878370.1"
                     /translation="MNLIIIGAQASGKMTIEQEVARQTGMNLFHNHDSIDFVLRFMPW
                     SQESTALIERMRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTHLTAAETAQLIIQKMTHIKEN"
     gene            1916..4564
                     /locus_tag="VOX58_001140"
     CDS             1916..4564
                     /locus_tag="VOX58_001140"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HES5878371.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAVVEPRLSTQWFVKMDELAKQAMDNQETDNRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIV
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVERLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFVANAKPEVIQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            4718..7126
                     /locus_tag="VOX58_001141"
     CDS             4718..7126
                     /locus_tag="VOX58_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HES5878372.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQAMSSLNWQDKSEWDYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDITDRPVYQLSTNMCAQHRLDLIAKIKTELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKAKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGMSHFLS
                     AEDYQTIKSLLKELQPFTVNVRENDPLFETTKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            7259..7987
                     /gene="cas5c"
                     /locus_tag="VOX58_001142"
     CDS             7259..7987
                     /gene="cas5c"
                     /locus_tag="VOX58_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HES5878373.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            7987..9882
                     /gene="cas8c"
                     /locus_tag="VOX58_001143"
     CDS             7987..9882
                     /gene="cas8c"
                     /locus_tag="VOX58_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HES5878374.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRSNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANREKLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            9887..10735
                     /gene="cas7c"
                     /locus_tag="VOX58_001144"
     CDS             9887..10735
                     /gene="cas7c"
                     /locus_tag="VOX58_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HES5878375.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLERRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYHQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            10737..11411
                     /gene="cas4"
                     /locus_tag="VOX58_001145"
     CDS             10737..11411
                     /gene="cas4"
                     /locus_tag="VOX58_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HES5878376.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMQEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            11408..12433
                     /gene="cas1c"
                     /locus_tag="VOX58_001146"
     CDS             11408..12433
                     /gene="cas1c"
                     /locus_tag="VOX58_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HES5878377.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGCASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILMTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            12444..12737
                     /gene="cas2"
                     /locus_tag="VOX58_001147"
     CDS             12444..12737
                     /gene="cas2"
                     /locus_tag="VOX58_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HES5878378.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   12885..13047
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12886..12916
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaa"
     gene            13208..13918
                     /gene="ccdA2"
                     /locus_tag="VOX58_001148"
     CDS             13208..13918
                     /gene="ccdA2"
                     /locus_tag="VOX58_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HES5878379.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHIFLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            13931..14554
                     /locus_tag="VOX58_001149"
     CDS             13931..14554
                     /locus_tag="VOX58_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HES5878380.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMVKKEITQDKMSMAVKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFTKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            14597..>14911
                     /locus_tag="VOX58_001150"
     CDS             14597..>14911
                     /locus_tag="VOX58_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HES5878381.1"
                     /translation="MEHKWKLWLFIVGVVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKVIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTG"
BASE COUNT         4725 a         2600 c         3229 g         4357 t
ORIGIN      
        1 cagttattaa taacatggca caagaggcct gccttatggt ataatggtaa agatgatttc
       61 agaaagagga agctaagcat gtcttatgaa aaagaatttt taaaagattt tgaagactgg
      121 gttaaaactc agattcaagt caatcagcta gccatggcaa ccagtcaaga agttgcccaa
      181 gaagatggcg atgaaagggc taaagatgcc tttattcgtt atgaaagtaa gttagacgcc
      241 tacgaatttt tactaggtaa atttgacaat tataaaaatg gtaaagcctt tcacgatatt
      301 ccagatgagt tatttggagc aaggcattat taaggacaag gagacacgaa atagtggcaa
      361 aacaaaaaaa tcgtaaaaaa gcattgaagt tagaaatgaa gcgtaatggc ttgttaaaaa
      421 aagcaggtca agtttttgat aaggcagttg aaagtgttga aacagccgtg gataaaacca
      481 tttctgctgg taaaaatctt gtggaaaaag gcagccaaac ggttgaaaac ctgacagcaa
      541 gcaaggaaag acttactctt gatgagtttg cagggcagcc agttgtagct ggtatccgct
      601 ctgaccttgt cgaaacccta tatgcagaag gcatccattc agcgcaagct tttaaggagt
      661 ggacagaaaa agacctcctt gctcttaagg gcattggccc agcaacggtt aaaaaattgg
      721 tcgaaaatgg cgcaagcttt aaaaaatagt catcttctcg ttgcttaatg gctataaatt
      781 tgttagaata ttagcaatca gaggtgcatt gagttggtct gttacagaga gcggtggtgt
      841 tgagaaatcc gcacagatgt caaggctggt agctgaggat aagaaatcag atatgaaaag
      901 tagcagatcc attttatggt ctgaaacagt ggtggtaccg cggaaatcgt aacacgaaat
      961 tcgtccctgt ccagtgtaag ctggataggg attatttggt ttcatttgaa agaagatgat
     1021 ggcttattgt taaaatgttg ttagaagtat acaatagaaa aactttgccc aaagtggaaa
     1081 cagaacggct gctattacgt caacgaaaag gggacaaggc attattccag aagcggcttc
     1141 agctctgatt gaaattggtt ttaccttact tgatttgcat aaaattgagc tgggttgcta
     1201 cgactacaac aagcaaagtc aggcagtggc tcgcaaatta ggctttacct tagaagccaa
     1261 tgtccgtgag cgtaaggacg ttcaaggtcg ccgctgtggc ggcatgcgtt ttgggttact
     1321 gagaagtgag tgggaggagc agaaacaaaa atgaacctta tcatcattgg tgctcaagcg
     1381 tcgggcaaga tgaccattga gcaagaagtt gccaggcaga caggcatgaa tttgtttcat
     1441 aatcacgatt ctattgattt tgttttacgg tttatgcctt ggtctcaaga gagcactgcc
     1501 ttgattgaac gtatgcgttt tgcttttttt gaaacctttg ctaagacagg tcaagacatg
     1561 atttttacta ttgtaattga cttcaatgat ccaaatgatg tagcaatgct tgaaaagatt
     1621 caggctgttt ttcagtctta tgaccaagag gttctctttg tcgaattaaa aacagatata
     1681 gaggaacgtc ttaaacgcaa tcgcacagaa aatcgtctca aacataaacc tcttaaacgc
     1741 aacatcgagt ggtcagaaca agatattcaa tccacgatgg catatgctgt ttttaatcca
     1801 gaagaaccac ccaaaacgct cacgcattac caaaaaatca ataataccca tctcaccgct
     1861 gctgaaacag cgcagcttat tatccaaaaa atgactcata tcaaggagaa ctaagatgac
     1921 agaactatca cctaaataca atcctgccga ggttgaggca ggtcgctatc aaaaatggct
     1981 tgatgcagat gtttttaagc catcaggaga ccaaaaagca aaaccgtatt ctatcgtgat
     2041 tccaccgcca aatgtgactg gtaagttgca cttaggacac gcctgggata cgacgcttca
     2101 agatattatc attcgtcaaa aacgcatgca gggctttgat acgctttggc ttccagggat
     2161 ggaccatgca ggaattgcga cacaggctaa ggtagaggag cgtctgcgcg agcaggggat
     2221 ttctcgttac gaccttggtc gtgacaaatt cctagacaag gtctgggagt ggaaagacga
     2281 atatgccact acgattaaag agcagtgggg caaaatgggg ctttctgtgg actacagccg
     2341 tgagcgcttc acccttgatg agggcttgtc aaaagcagtt cgcaaggttt ttgttgacct
     2401 ctacaaaaaa ggctggattt accgtggtga attcatcatc aactgggatc ctgctgccag
     2461 aactgccctt tctgacatcg aggttatcca caaggatgtt gagggagctt tctatcacat
     2521 gaactatatg ttggaagatg gctcacgcgc ccttcaagtg gcaacaacgc gaccagaaac
     2581 catgtttgga gacgttgccg ttgccgtaaa cccagaagat cctcgttaca aagatttgat
     2641 tggcaaaaat gttattttac caatcgtgaa taaattgatt ccaatcgtgg gtgacgagca
     2701 tgctgaccct gaatttggga ctggagttgt gaaaatcacg cctgcccatg atcctaacga
     2761 tttcgaggtt ggtcaacgcc ataatctacc acaagtgaat gtcatgaacg atgacggtac
     2821 catgaatgag ttagctggcg actttgcagg catggaccgt tttgaagctc gtcaagctac
     2881 cgttgctaaa cttgaagaac ttggtgccct agtaaacatc gaaaaacgtg ttcactcagt
     2941 aggtcactca gaacgttctg gtgcagtcgt tgagcctcgt ctatccaccc aatggtttgt
     3001 aaaaatggat gagcttgcta aacaagctat ggacaaccaa gaaacagata accgagtaga
     3061 tttctaccca ccacgcttta atgatacctt ccttcaatgg atggaaaatg tccatgactg
     3121 ggtaatctcg cgtcagcttt ggtgggggca ccaaattcct gcttggtaca atgcagaagg
     3181 tgagatttac gttggcgaag aagcaccaga aggtgatggc tggactcaag atgaagatgt
     3241 tcttgacact tggttctcat ctgctttatg gccgttctca actatggggt ggcctgacac
     3301 agatgtagaa gatttcaaac gttatttccc aacctcaacc ttagtaacag gttatgacat
     3361 cattttcttc tgggtgtcac gtatgatttt ccaatcgctt gaatttaccg ggcgtcagcc
     3421 attccaaaat gtcctgattc acggtcttat tcgtgacgaa gaaggacgca aaatgtctaa
     3481 atcattgggg aacgggattg atccgatgga tgtcattgag aaatatggtg ccgatagctt
     3541 acgttggttc ctctcaaacg gctctgcccc aggacaagac gtgcgtttct catacgaaaa
     3601 aatggacgcc tcttggaatt tcattaataa aatttggaat atttctcgct atatcctcat
     3661 gaataatgaa gggttgacct tggaagaagc tgaaagtaat gtggctaagg tagctgcctc
     3721 agaagctgga aatgtgactg accaatggat tctccacaat ctcaatgaaa ccatcgctaa
     3781 agtgacagaa aactttgaca agtttgagtt tggtgtggca ggacacattc tttacaactt
     3841 catctgggaa gaatttgcca actggtatgt ggaattgacc aaggaagtgc tatatagcga
     3901 caacgaagct gaaaaagtta tcacgcgctc tgtccttctt tatactttgg acaagattgt
     3961 acgtctcctt cacccaatca tgccatttgt aacagaagaa atttacgctc aatatgccca
     4021 aggttctatt gtgacggtgg actatcctgt ggttcgccca gcttttgaaa atgaagctgc
     4081 gcataaaggt gtggaaaggt tgaaagacct gattcgtgca gttcgtaatg cgcgcgctga
     4141 agttaacgtg gcaccatcaa aaccaatcac cattttggtg aagacagcag atagtgagtt
     4201 ggaagacttc ttcaccagca acgtgaacta catcaaacgt ttcaccaatc ctgaaaaatt
     4261 agaaatcagc tcagccattg ctgcgccaga gctggctatg accagtatca tcacaggtgc
     4321 tgaaatctac ttgccactag cagatttgct taacgtggaa gaagaactcg ctcgcctaga
     4381 taaagaactg gctaaatggc aaaaagaatt ggacatggtc ggcaaaaaac tcggaaacga
     4441 acgctttgta gccaacgcca aaccagaagt catccaaaaa gaaaaagaca aacaagccga
     4501 ctaccaagcc aaatacgacg caacccaaga acggattgca gagatgaaga aaattaaatc
     4561 atagtgttta gaacgcagtt gaaaaactgt gttcttttct tactaataga gatattagat
     4621 tgaagtttaa ttatgagaaa aacgctttct ttatttaaag agttatgtta caatactatt
     4681 acaatcatcg tacaaatagt gttattttga ggggcatatg agaatgattt tagcacacta
     4741 tgactgtaaa aaagataaaa agcaatcttt agatgagcat ttatggcatg tggcctgttc
     4801 tagtcgacag gaagcatcta taattggtca aggagatgtg ctttttttaa ttggtcttta
     4861 ccacgacctg ggcaaagctg atcgaacctt tcaagataaa ttattaaata atccaaatcg
     4921 gcatgttgat cactcttatg caggggcaaa atacttatgt tctattattg ggcctcatct
     4981 aaaaaaccga ggggttgata aaaatgagag aatgacattc aacgaaatgg tggggtatgt
     5041 catctctgct catcatggga tgtatgattt atgctactat tttgacgatg ctgaatatta
     5101 tggctttaat aagtttaaaa atcgtatcaa tagagactta gatggttatc actatcatga
     5161 agatattaaa gggtacgctc taaaattaga aaaaaaatta tgtgattatg gctacaaaga
     5221 tttaagggag cttattgata aagcttttga taattaccaa caagccatgt cttccttaaa
     5281 ctggcaagat aagagtgagt gggattatta tcagtcttgt atggtgagac tttacttgtc
     5341 actcttaaaa aacgctgata ttttggacac agtaaatgcc tatggcctta agataagtcc
     5401 tatggataaa acagagcgat cctttctaaa acactcctat ttagcggcca ttgaacaaaa
     5461 atatgctagc tttggacagc caaacaatca gttgaacact attcggacag aaatcgctga
     5521 gcgtgttaaa gaaagaggta aacgagattc caaggggatt tatcgcttag atttaccgac
     5581 aggagctggc aagactaatc ttagtatgcg ttatgcgttt caccaattag ttcatcacga
     5641 caaatcaagg tttttttaca taacgccctt tctttcggtt cttgaacaaa atgcttccga
     5701 aattagaaaa gttacaggtg accttggcgt cctagaacac cattccaatg tggtgaaaca
     5761 ggctaatgaa gatgatgatg acaaggacag tttattgtca gcttatctta gtgatagctg
     5821 ggacagtcaa gtagtcttga cttctatggt tcaatttttc caaacacttt tcaaaacaaa
     5881 atcagctaat ctgagacgtt tttcaagttt gattaatagt gttgtgattc tagatgaagt
     5941 tcaatccctg cctattgaag tcaccacttt gtttaattta acgatgaatt ttttaaataa
     6001 agttatggat acaaccatcg ttctttgcac agcaacacaa cctgcttatg attcttcaga
     6061 gattgaccat cgtatctgtt atggagggaa cttgggagaa ttagctgaaa tcgttgagtt
     6121 aacgattgaa gaaaaacaga ttttttcaag gacagagctt agaaaatttg atgatagtga
     6181 tcagaaagtt cacttgactg atgttattaa tcttattcta ggtgaggaaa actcagttct
     6241 tgctattttt aatacgaaaa aaacggttca taactgctat actatgctaa aagacattac
     6301 tgatagaccg gtctatcagc tttcgacaaa tatgtgtgcg cagcatagac ttgacttgat
     6361 tgctaagatc aaaacggagt tacaaaataa tatccctatt atttgtatta gcacgcaatt
     6421 aattgaagca ggtgtagatg ttgattttca tcgcgtcatt cgttcctact cagggattga
     6481 ttctattgtt caggctgctg gacggtgtaa ccgagaaggc aaacgagata aagggcaagt
     6541 cactcttgtc aatctgacca atgaagagga aaatatttct aggctgacag aaataaaagc
     6601 taaaaaagaa gccacagaat ctattcttca taagattggg tctccaattg atatctcaac
     6661 tttaaaccgt gacttttttg agtattatta tgccaataat cagggactga tggattatcc
     6721 tttggaagac aacctatcaa tctacgacta tttaagcctt aatatttatc agacggcaaa
     6781 taaaaagttc aaaggtaagt taaaacaagc ttttaaaaca gcaggagcca aaatgaacct
     6841 catcaataat gatatgatag gaattctcgt accttatggc gaagctgaga aaaaattggc
     6901 ttatttagaa gaattgggta tgtctcactt tttatcagca gaagattatc aaacgataaa
     6961 atcattacta aaagagttac aaccttttac ggttaatgtc cgcgagaacg atcctctctt
     7021 tgagacaaca aaatcttatc taaatggtca gattctggtt ttgacgtcgg agtattatga
     7081 cacggaaaga ggagttaaat acgattcagc tagcttttac ttctaactca aaacgaaagc
     7141 agattaacaa aaggttgtta gaggaccttg ttaacctgcc aatcatcatt aataattatt
     7201 atcaatttag actatttaat aaaattagat tacaaaaaaa cagaaggagg aaagtagctt
     7261 gtacagatct agagacttct acgtgagagt aagtggtcag cgagctcttt ttacaaatcc
     7321 agccacaaaa gggggatcgg aacgctcatc ctattcggtt ccgactagac aggcactgaa
     7381 tggtatcgtt gatgccatct attataagcc gacctttact aatatcgtca cagaggttaa
     7441 ggttattaac cagattcaaa ccgaattaca gggtgtcagg gctctgttac atgattatag
     7501 tgcagattta agttatgtat cctatttgag tgatgttgtt tatctgatca agtttcattt
     7561 tgtttggaat gaagatagaa aagatttgaa ctcagataga cttccagcta aacatgaagc
     7621 cattatggag cgttctattc gtaaaggggg acgtcgagat gtgtttttgg gtacaagaga
     7681 atgtttaggg cttgtagatg atatcagcca agaagagtat gagactactg tgtcgtatta
     7741 taatggtgtc aatatcgact tgggaatcat gttccattcc tttgcctatc cgaaggacaa
     7801 aaagacacca ttaaaatcat actttacaaa gactgtgatg aaaaatggag tcattacgtt
     7861 taaagcacag tctgaatgcg atattgttaa cacgctttct agttatgctt ttaaagcacc
     7921 agaggagata aaatcggtta acgatgaatg catggagtat gatgccatgg agaaaggaga
     7981 aaactgatgg atttttttac ttctctcttg aagacttatg aaaaagcaga gctagcagac
     8041 ttggttgatc accaaaaaag aagtaatgag ccggttttac tgccgattta tcatacgagt
     8101 ttaaagtcaa atggcaaaaa tatcatttca gtgaaacttg acaaagatgg ccagtttcac
     8161 aaggcagaat ttatggcaga taagcaaatg attatttttc ctgtaacggc tgattctgtt
     8221 gctaggtcag gtagtcatcc tgcaccgcat cccctagtcg ataaatttgc ttattatagt
     8281 gctgaaatgg ggcagtctca gtatgattct ttccataagc aacttaataa ctggattgat
     8341 tattgtgagg agggtgacgt caagaggttt ttaaccattg ttcagcaatt tattttgaaa
     8401 ccagaatttc tgacactgat tctcgattct ttaattggtc ctgattatca aaataatcaa
     8461 ttaaaagtca cattttgtga tgccactgga aaagaaaaat taattgattt atcagcttgc
     8521 tttttagaat tttcaattga tcagttccag ggctttaaaa atgaatcggt ttcgacattt
     8581 aaaactttac accaatccta tatttctttt gttgaagcca atcgtgaaaa gcttggtatt
     8641 tgtaatatta gtggacgaga ggaacagctt accgataagc atagaggttt gatggggaat
     8701 gctaaaatca tctctgttag taataaaaga gaagcttata aaggacgttt tagagaacgt
     8761 gaagatgttt ttagtgttgg ctatgaaact tccgaaaaga ttcatttaat gctcaagtac
     8821 cttttagaaa ataaaaatac cagtacttgg ttagggtctt ctcaatattt aatcaactgg
     8881 tttagtgatg atttaacaaa tgatagtcgg ttggatatcg tatcaccaat ctttgatgac
     8941 ggacttgaag aagatgatga tgacgatacg cctcctgtta taacattagc aactgaagac
     9001 aataaaagaa ttggtaaatc attcatcaag ggacaaaaat tatttgctaa tgatgccact
     9061 tactacgttg ctattttgaa taaaaccagc aatgggcgga ttgctttaaa atattttcgt
     9121 cagcttcaag cgtcccaatt actcaccaat cttaacaagt ggcaggaaac atacagttgg
     9181 gagtcgcgat ctaagtttgg aaaaagtcgc ttaagaaccc ctacttttca tgacatcctt
     9241 aatgtgtcct acggggttga tagggatcgc ttccttgaat tagataatga taacttcaaa
     9301 agtgatcaaa ttcaaaagtt agtggcaagt ttgattgatg gtaaaccgat gccacagtcc
     9361 attgtcaaaa agttaggtaa caatgttaaa gaacgacatc gttaccgtaa gcactggtat
     9421 caagttgagc aggtctgctt agcaatttta cacaaacaaa atggggagga attttcaccg
     9481 atgctagatc ataccaatca aaatcgttcc tatctttttg gacgattatt agcaattttt
     9541 gaattaatcg agaccttgcg ttatggcttg gatggaaaca ataacgaccg tattaccaat
     9601 gctgaacgtt attggacagc ctatactgga caaccaacaa aattgatgat gttattggaa
     9661 aataaaatta agccttacga agaaccattg aaattaaatc gtcgtggcag ttggatgaaa
     9721 ttagaaaaag aaaaagaaga gattttagaa ctgttaaatc ctctgttaga aacagaaaca
     9781 atggaaaaac ccttagatta ccgctttatt tttgggtatt atgctgagaa aaactattac
     9841 tatacaaaac aaaacactga agtaacagaa agtgaggagt aaaaagatgt tggaacacaa
     9901 aattgatttt atggtaactc ttgaagtgaa agaagcaaat gcaaatggtg atcccttaaa
     9961 tggaaacatg cctcgtacag atgccaaagg atatggtgtg atgagtgatg tctccatcaa
    10021 acgtaagatt cgtaatcgtt tgcaagatat ggggaagtct atttttgtgc aagctaatga
    10081 acgtattgaa gatgactttc gttcactaga aagacgcttt tcacaacatt ttacagctaa
    10141 gacacctgac aaagaaattg aagaaaaagc aaatgcatta tggtttgatg ttcgtgcttt
    10201 tggacaagtt tttacttatc tgaaaaaatc aattggggtg cgtggaccag tttccatcag
    10261 tatggctaag tccttggagc caattgtcat ttccagcctt caaattacgc gtagtaccaa
    10321 tggtatggaa gctaagaata atagtggccg ctcttctgat acgatgggga caaaacattt
    10381 tgtagattat ggtgtgtatg tacttaaagg ttctatcaat gcttattttg ctgaaaagac
    10441 tggtttttct caggaagatg ctgaggctat taaagaagtt ttggttagct tgtttgaaaa
    10501 tgatgcgtcg tctgcacgtc cggaaggctc tatgcgagtt tgtgaagtct tttggtttac
    10561 gcattcaagc aaactgggga atgtttcaag tgcgcgtgtc tttgacttgt tagagtatca
    10621 tcaatcaata gaagaaaaaa gcacttatga cgcttatcag attcatctaa atcaagaaaa
    10681 attggctaaa tatgaagcga aagggttaac ggttgaaatc ctagaaggac tctagtatgg
    10741 tctatgccga agatgattat ttaatgctat caggtattca gcatttccaa ttttgtaaac
    10801 gtcaatgggc gttgatccat attgagcaac aatggcttga taatgaagcg acagcgcatg
    10861 gacaggtttt acatactaaa gcagataacc cttacattaa agaaaaacga aaagagcttt
    10921 tggtctcacg tgctatgccc atttcttctg cagaacttgg actttcagga attatggatg
    10981 ttgtggaatt ttataaagat gatcaaggtg tgtctttgag gggaaaacgt gggaaatggt
    11041 taccaaaagt tgtggaatac aagcgcggaa aacctaaaaa agataccaga gatattgtcc
    11101 agttggtggc tcagaccatg tgtttagaag aaacgctaga ctgcgacatt aacgaaggtt
    11161 gtctttatta ccatagtgtc aatcaaagag tgattgttcc tatgacatca gctttgcgtc
    11221 aagaagtgaa ggaattagcc gcagagatgc aagaggttta tcagagtcaa atgctaccta
    11281 aagcagctta ttttaaaaac tgtcagcttt gttctttagt cgatatttgt aagcccaggt
    11341 tgagtaaaaa aacaaggagt gtgtcgcgtt acatcaatga ggctatgacc agtgaggaga
    11401 tggacctatg aaaaagttgc taaatacctt gtatttgacg caagaagatt tttatgtcac
    11461 taaagagggc gataacattg ttatcaagca agaaggtaag gttctcaaac ggtttccgtt
    11521 tcggattatt gacggtattg tctgtttttc ttatttgggt gtgtcgcctg ctttggtgaa
    11581 gttatgtacg gagaatcaga ttaatttatc gtttcataca ccacaagggc gtttttgtgg
    11641 tcgctatatt ggttcaacca atgggaatgt gttgttgcgt agagaacatt atcgtttatc
    11701 tgatcgtgag gaatctttgg aatacgcaaa gcggtttatt ttggctaaaa tttccaactc
    11761 aaggaaatac ttgctacgct ttaaacgaga tcatcgtcaa cagattgata ccaagctttt
    11821 tgaggctgtt aatgacgaat tgatatgggc tttagagatg gttcaggcag cagataataa
    11881 agactcttta agagggattg aaggccaagc tgctaatcag tattttcgca tatttaatga
    11941 cctggtgttg acggacaaaa aaacgtttta cttccaaggt cggagtaaac gaccaccctt
    12001 agattgtgtt aatgccctct tgtcttttgg ttacagttta ctgacctttg aatgtcaatc
    12061 tgccttggaa gctgtcggat tagacagtta cgttggtttc tttcacacgg atcgtcctgg
    12121 gtgtgctagt ttagcgcttg atttagttga agagttccgc tcatatattg tagatcgttt
    12181 tgtcttttca ttaattaata aaggacaact tcagaaaaaa cactttgagg ttaaagaaaa
    12241 tggtagtatt ttaatgacgg aaaatggcag agctattttt attgatttgt ggcagaagcg
    12301 taagcatact gaggtagaac atccttttac aaaagagaaa gtaaaactta tgttattacc
    12361 ctatgtacaa gcgcagcttt tagctaaggc catacgagga gatttagaaa gctatccacc
    12421 ttttatggtt taggagatgt tatatgatgg ttttagtcac ttatgatgta aatacggaaa
    12481 cacctgctgg tagaaaaaga ttgcgtcatg ttgccaaact ctgtgtggac tatgggcaac
    12541 gtgttcaaaa ttctgttttt gaatgttctg tgacacccgc agaatttgtg gatataaagc
    12601 accgcttaac acaaatcatt gatgagaaaa ctgatagtat tcgcttttat ttattgggga
    12661 aaaattggca gaggcgtgtg gaaacacttg gtcgctcaga cagctatgac ccagataaag
    12721 gtgtcttatt attgtaaaaa tctcttgtgc gaagctagct ttcacagaaa caccttgctt
    12781 gctcgcgcaa aaataactta aaaaagaagc gaaatggaga taaaaaggct taaaacacct
    12841 ctatgtgtgc catccgtttc tctataaact gtgtcatttg gcgcagtctc acccttcatg
    12901 ggtgagtgga ttgaaataaa taattacatg actagccaat acacccacat agtctcaccc
    12961 ttcatgggtg agtggattga aatttcatga gcttctttac tctcaaagta agagtggtct
    13021 cacccttcat gggtgagtgg attgaaagat gaacccgacc tctcttttaa tgaggtcggg
    13081 tttttacatg cctttagtca accatttatc tgatagcgta tcaaattcaa gtttttgcat
    13141 gttctagtct atggtttgcg ccactagctt tcggtaaact aagggtaact taagagagga
    13201 gttatttatg acactatcac ttgtattaat ggtatcggtt tttggggcag gcttgttatc
    13261 ctttttctct ccgtgtatct ttccggtcct tcctgtttat ttaggcattt tactggatgc
    13321 agatgattca aagacgatta ctatatttgg taaaaaactc tattggtatg gcattgtcaa
    13381 aaccttagcc tttatttttg gtctatctac tatttttgtg attttaggtt acggggctgg
    13441 ttttttagga aatatccttt atgcggtatg gtttcggtac ttactgggag ccttggtcat
    13501 tatcttgggg attcatcaga tgggcctcat taccattaag agcttacaat ttcaaaaatc
    13561 actgactttc cataataaca agaatcgcaa cggtttgttc aatgcattta tccttggttt
    13621 aacctttagc tttggttgga cgccttgtgt gggacctgtt ttgagttctg ttctagcttt
    13681 ggtggcttca gggggaaatg gtgcctggca aggtggcgtt ttgatgatta tttatactct
    13741 tggattgggc attcctttcc tactcatctc ttttgcgtca ggcattgttt tgaaacagtt
    13801 taacaagctc aaaccccaca tatttttact gaaaaaagta ggaggcgttc tgattatagt
    13861 catgggaatc ttgcttatga caggaacctt aaataactta gcacaacttt ttggataaag
    13921 gagaaacaca atgaaaaaag gactattagt aacaactggt ttggcttgtc tcgggctact
    13981 aactgcttgc tcaacccaag acaatatggt taaaaaggaa ataactcagg acaagatgag
    14041 catggcagtt aaaaagaaag ataagatgtc aacatcaaag gacaagtcca tgatggcaga
    14101 taaatcatct gataagaaaa tgaccaatga tggtcctatg gcgcctgatt tcgaactcaa
    14161 aggcatcgat ggtaaaacct atcgcttatc agaattcaaa ggtaaaaaag tttatttgaa
    14221 attttgggcc tcttggtgtt caatctgttt atcaaccttg gcagataccg aagacctggc
    14281 taaaatgtca gataaagact atgtggtctt aaccgtcgtc tcgccaggtc atcaaggaga
    14341 aaaatcagaa gcagacttta caaaatggtt ccaaggaaca gactataagg acttaccagt
    14401 cttgttagat ccggatggca agctattgga agcttacggc gtcagatctt acccaacaga
    14461 agttttcatt ggaagtgatg gtgttcttgc taaaaaacac attggttatg ccaaaaaatc
    14521 agacatcaaa aagaccctta aaggtataca ttaggaagga ccagctgata ggcattagaa
    14581 aaaaggagtt gagtttatgg aacacaaatg gaaattatgg ctatttatcg ttggagtggt
    14641 cttgttaata ggcggagctt ctgttctcat tcagaaaggt caagccaagg cttttagtca
    14701 aggcaagcct caactaccag ctaaaaaagt caaatccact aaagtagatc ccaatgcccc
    14761 tgttgatcaa aaagtgattt acctggctgg aggctgtttc tggggcgttg aagaatattt
    14821 ttcacaagtg gatggcgttc tagatgcggt gtcaggctat gccaatggta gaggcgatac
    14881 caccaactac cagttaattc atcagactgg t
//
LOCUS       DAUVUY010000045        14898 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.045, whole genome shotgun sequence.
ACCESSION   DAUVUY010000045 DAUVUY010000000
VERSION     DAUVUY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14898
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.045"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..620
                     /locus_tag="VOX58_001151"
     CDS             <1..620
                     /locus_tag="VOX58_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HES5878382.1"
                     /translation="WLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVYRM
                     SEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQVF
                     VILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNMIS
                     PTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            637..1968
                     /locus_tag="VOX58_001152"
     CDS             637..1968
                     /locus_tag="VOX58_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HES5878383.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPEGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTDPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYKG
                     NKLEALQKELDKLGFEYVVKEGELTVYGLAQHTKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            1988..2938
                     /gene="arcC"
                     /locus_tag="VOX58_001153"
     CDS             1988..2938
                     /gene="arcC"
                     /locus_tag="VOX58_001153"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HES5878384.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALISTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKSLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYINFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
     gene            3251..4243
                     /locus_tag="VOX58_001154"
     CDS             3251..4243
                     /locus_tag="VOX58_001154"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HES5878385.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            4366..4905
                     /gene="rsmD"
                     /locus_tag="VOX58_001155"
     CDS             4366..4905
                     /gene="rsmD"
                     /locus_tag="VOX58_001155"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HES5878386.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            4895..5386
                     /gene="coaD"
                     /locus_tag="VOX58_001156"
     CDS             4895..5386
                     /gene="coaD"
                     /locus_tag="VOX58_001156"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HES5878387.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            5373..6410
                     /locus_tag="VOX58_001157"
     CDS             5373..6410
                     /locus_tag="VOX58_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HES5878388.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDTDFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVKNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            6825..7802
                     /locus_tag="VOX58_001158"
     CDS             6825..7802
                     /locus_tag="VOX58_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HES5878389.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDCVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTMYVCDSQNFDFESIQ
                     LEASYNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            8226..8870
                     /locus_tag="VOX58_001159"
     CDS             8226..8870
                     /locus_tag="VOX58_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HES5878390.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            8874..9953
                     /gene="rlmN"
                     /locus_tag="VOX58_001160"
     CDS             8874..9953
                     /gene="rlmN"
                     /locus_tag="VOX58_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HES5878391.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(10035..10661)
                     /locus_tag="VOX58_001161"
     CDS             complement(10035..10661)
                     /locus_tag="VOX58_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HES5878392.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQLQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            10889..11416
                     /locus_tag="VOX58_001162"
     CDS             10889..11416
                     /locus_tag="VOX58_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HES5878393.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            11558..11761
                     /locus_tag="VOX58_001163"
     CDS             11558..11761
                     /locus_tag="VOX58_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HES5878394.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            11766..12737
                     /locus_tag="VOX58_001164"
     CDS             11766..12737
                     /locus_tag="VOX58_001164"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HES5878395.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            12747..13136
                     /locus_tag="VOX58_001165"
     CDS             12747..13136
                     /locus_tag="VOX58_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HES5878396.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRAKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            13319..>14898
                     /gene="typA"
                     /locus_tag="VOX58_001166"
     CDS             13319..>14898
                     /gene="typA"
                     /locus_tag="VOX58_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HES5878397.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEG"
BASE COUNT         4778 a         2567 c         3128 g         4425 t
ORIGIN      
        1 cttggttaac cacaatgcct atcttagggg gagttattgg gaaaaccatg ggagcatttg
       61 gtacttggta tttcccagaa atcaccatgc tctttatcat gatgggtgtc ttagttgcta
      121 ttgtttatcg tatgagtgaa gaagactttt ttagctcttt tttgactggt gcaggtgaat
      181 ttttaggtgt tgccatgatc tgtgctattg cacgcggtat tcaagttatc atgaatggtg
      241 gtatgattac agccactatc ttacacttag gtgaaacaag tctttctggt ttatcttctc
      301 aagtttttgt gatattagct tatattttct acctcccaat gtccttcttg attccatcaa
      361 catcaggact tgctggggct acaatgggga ttatggcacc gcttggacaa ttctcaaatg
      421 tccctgctca ccttgttatt acagccttcc agtcagcttc tggaatctta aacatgattt
      481 ctccaacttc agcaatcgtt atgggagcac ttgcgcttgg tcgcgttgac cttggtactt
      541 ggtggaaatt cattggtaaa tttattgtaa tggtgatgct tgtaagcgtg ctattacttg
      601 tagttgcaac attcttttaa cagataggag gttcctatgg aatcctatat cacacctaaa
      661 catcaagagg cctgtgtagc tgccattaaa cagatcgttt cttacccttc tgtttgtcat
      721 gaaggagaaa atggcacacc ttttggacag gccattcaag atgttctcga agcaacctta
      781 gacttatgtc aaggtcttgg ttttcatact tatattgatc ctgaaggtta ctatggctat
      841 gctgagttag gtgaccaaaa agaggtcttg gctattcttt gccatttgga cgttgttcca
      901 gaaggagatc gtcagttatg gaagacagat ccttttgact gtgttgaggc agacggctgt
      961 ttatttggtc gaggcactca agacgataaa gggcctacta tgatggcgct atttgccacc
     1021 aaagccttga tagatgcagg tgttaccttt aataaacgca ttcgttttat tttcggaaca
     1081 gatgaagaaa ctttgtggcg gtgtatgaat cgctacaatg atgtagaaga acaggctacc
     1141 tttgggtttg ctcctgattc aagtttccct ttaacctatg ccgaaaaagg cctacttcag
     1201 gcaaaattag tggggaaagg ttctgataaa ctgtcacttg aaatcggaca agcctacaat
     1261 gttgttcctg caagagcttc ttataaaggc aataagcttg aggctttaca aaaagaacta
     1321 gataagcttg gctttgagta cgtcgtcaaa gagggagaat taacggttta tggtctcgcc
     1381 caacatacta aagatgctcc cgatggcatc aacgctatga ttaggcttgc caaggcttta
     1441 gtggtccttg aacctaacaa aacccttgat tttcttgcta atgtcattga tgaagatggc
     1501 agagcactta atatttttgg cagaatcgaa gatgaacctt caggcaaatt aagctttaat
     1561 gccgcagggc ttaccttaac caaagacaaa gcagaaattc gcctagatat tcggattcca
     1621 gttttggcag ataaggaaaa attagtccaa caattaagcc aaaaagcaca agaatacggc
     1681 ctaacctatg aagaatttga ttatttggcc ccattatatg tgccattaga cagtgaattg
     1741 gtaacaacct tgttatctgt ctatcggaaa aaaacaggtg atcaaagccc tgcccaatca
     1801 tcaggagggg caacctttgc tagaacaatg aagaattgtg ttgcctttgg tgcccttttc
     1861 cctgatgctg ttcaaacgga acaccaagaa aatgaacata ttgttttagc ggatgcttac
     1921 cgcgctatgg atatttatgc ggaagctatt taccgactaa cgcgataaca agatatgagg
     1981 agtaattatg acgaaacaaa aaatcgtagt cgcacttgga ggaaatgcta ttctttcaac
     2041 agatgcgtca gcaaaagcac aacaagaagc cttgatttcc acatcaaaat cattggttaa
     2101 attaattaaa gaaggtcatg aagtcatcgt gacccacggc aatggcccac aagttggtaa
     2161 tctcttatta caacaagcag cggcagattc tgaaaagaat ccggctatgc ctttggacac
     2221 ttgtgttgcc atgacagaag gtagcatcgg tttttggtta gttaatgccc ttgataatga
     2281 attgcaagcc caaggtattc aaaaagaagt ggcagctgtt gtcactcaag tgatcgtgga
     2341 tgccaaagac ccagcttttg aaaacccaac aaaaccaatt ggtccatttc ttaccgaaga
     2401 agatgccaaa aaacaaatgg cggagtctgg tgcaagtttc aaagaagatg caggtcgtgg
     2461 ctggcgtaag gttgtgccat caccaaaacc agttggtatt aaagaagcca atgtcatcag
     2521 aagtctcgtt gattctggcg ttgttgtggt tagtgctggt ggtggcggtg tgcctgttgt
     2581 ggaagatgca actagcaagt ccttgactgg tgtggaagct gttattgaca aggactttgc
     2641 tagccaaacc ctttcagaat tggtggatgc agacctcttt atcgttttga caggagttga
     2701 caatgtttac atcaacttca acaaacctga ccaagccaaa ttggaagaag tgactgtctc
     2761 tcaaatgaaa gaatacatca cacaagacca atttgcacca ggtagtatgt taccaaaagt
     2821 ggaagctgcc attgcttttg ttgaaaacaa accaaatgct aaagccatta tcacatcgct
     2881 agaaaatatt gacaatgtac tatcagcaaa cgctggaaca caaattatcg cagggtaatc
     2941 aagaggattt ttagttctgt ttaaaagact catttaatgg tattttctga agagtacgac
     3001 tgacataaag agttgatact gtttttaaag gaaaagagtg tgccataata aaaaacaata
     3061 gtagtattag aaaaggctaa ttttattgag aattagcctt ttcttttagc tttgataggt
     3121 gtataataac catactattt tttatttttc aaaatattag aataaacaaa agtggacaac
     3181 gtataaagag aactgctaag tcgttgagtt cattagtccc aaaaagatta ccaaaaatga
     3241 aggaggagct atgaagaaaa gttttattca tcaacaagag gagatttcct ttgttaagaa
     3301 tacgttcact cagtatttga ttgctaagtt agatgttgtt gaagtgcaag gacccatttt
     3361 aagtcgggtc ggggacggta tgcaagataa tttgtccggt actgaaaatc cagtatctgt
     3421 caatgtcttg aaaattccta atgcaacctt tgaagttgtg cattcattag caaaatggaa
     3481 acgtcacacc ttagcccgtt ttgggtttaa tgaaggtgaa ggccttgttg ttaatatgaa
     3541 ggctctccga cctgatgagg attcacttga ccaaacccat tctgtctatg ttgaccaatg
     3601 ggactgggaa aaagtcatcc ctgatggcaa acgtaatctc gcttacctca aagaaaccgt
     3661 tgaaaccatt tacaaggtca ttcggttaac tgaattagct gtagaagctc gttatgatat
     3721 tgaggctgtt ttacctaaga aaataacctt cattcatacc gaagaattgg tagctaaata
     3781 ccctgatttg acgcccaaag aacgtgaaaa tgccattact aaagaatttg gagcagtctt
     3841 tttaattggt attggcgggg ttttacctga cggtaaacca cacgatggcc gcgctcctga
     3901 ctatgatgac tggacaacag aaactgaaaa tggctatcat gggcttaatg gtgatatttt
     3961 ggtttggaat gaccaacttg gttcagcctt tgaattatcc tcaatgggga tccgtgtgga
     4021 tgaagaggcc ctaaaacgtc aggtggaaat gactggtgac caagaccgct tagcctttga
     4081 ttggcacaaa tctttgttga atggactttt ccctctaacg attggtggag gaatcggtca
     4141 atcacgtatg gtcatgtttt tgttgcgtaa aaaacatatt ggagaagttc agacctctgt
     4201 ttggccccaa gaagttcgtg atagctacga taatatttta taagtaatca catcattttg
     4261 ctaagttttc cacttgtata agagacttta aataaatgat cagaaatatt gaaaaaagaa
     4321 atcattttta aggtttagag ctagaaattt gctataataa atattatgag agttgtatca
     4381 ggtgaatttg gtgggcgtcc tttaaagacg ctcgatggaa agataacacg ccctacttca
     4441 gacaaagtta gaggtgcgat ttttaatatg ataggacctt actttaatgg tggtcgtgtt
     4501 ttagatttgt ttgcaggttc aggtggatta gccattgagg ctgtttctcg tgggatgtct
     4561 gctgcagttt tggttgagaa gaatcgtaaa gcgcaagcta ttattcaaga caatattata
     4621 atgactaaag ctgagaatcg gtttaccttg ttaaaaatgg aagctgagcg agctattgat
     4681 tgcttgactg gtcgatttga cttagtcttt ttagaccctc cttatgccaa agaaacaatt
     4741 gtggcaacta ttgaagcttt agcagctaaa aacttattga gtgaacaggt tatggttgtc
     4801 tgtgagacag ataaaacagt cttgttgcca aaagagattg caacgttagg tatttggaaa
     4861 gaaaaaattt atggaattag taaggtaaca gtttatgtta actaaaatag gactttatac
     4921 agggtcattt gatcctgtta caaacggtca tctcgatatt gttaaacggg caagtggcct
     4981 atttgatcaa atatatgttg ggatttttga taatcctacc aaaaaaagct attttaaact
     5041 agaagttcgt aaagcaatgc ttactcaagc tctggctgat tttacaaatg taattgtagt
     5101 aacttcgcac gaacgtttag ctattgatgt agctaaagaa ttacgagtaa ctcacttgat
     5161 tagaggcttg cgaaatgcaa ctgactttga gtatgaagaa aatttggaat attttaatca
     5221 ccttttagct ccaaatattg agaccgtcta tttgatatca agaaataaat ggcaggccct
     5281 tagctctagt cgtgtgagag agctaattca ttttcagtct tccttagaag gtttggttcc
     5341 tcagtcagtt attgctcaag tggagaaaat gaatgaaaag acttaaaaaa atcaaatggt
     5401 ggttagtggg tctgctagct ttaatctctt tgttgctagc gttatttttt ccgctacctt
     5461 attatattga aatgcctgga ggcgcttacg atattcggac tgtcttacaa gtcaatggca
     5521 aagaagacaa acgaaaagga gcttaccagt ttgttgcagt gggcattagt cgtgctagcc
     5581 tcgcgcagct attatatgct tggctgacac cgtttactga aattagtaca gcagaagata
     5641 caacaggcgg atacagcgat actgatttcc ttcgaattaa tcaattttac atggaaacat
     5701 cacaaaatgc agctatttat caagctttat ccttagctgg aaaaccagtt acattagatt
     5761 ataaaggcgt atatgtttta gacgtaaaga acgaatctac ttttaaagga acgttacact
     5821 tagcagatac tgtaacaggt gtaaatggta aacagtttac tagttcagca gaacttattg
     5881 actatgtttc tcacctaaaa ctaggggatg aagttacggt tcagtttacg agtgataata
     5941 agcctaaaaa aggagttggc cgtattatca aactgaaaaa tgggaaaaat gggattggca
     6001 ttgccttgac tgatcataca agtgtcaatt cagaagacac agtgatcttt agtactaaag
     6061 gagtaggagg acctagtgct ggtctaatgt ttactcttga tatatatgat caaataacta
     6121 aagaagattt acgcaagggc cgtacaattg caggtacagg aactattggc aaggatggcg
     6181 aagtaggaga tattggtggt gcaggtctta aagtagttgc agcagctgaa gctggtgcag
     6241 atatattttt tgttccgaat aatcctgttg ataaggaaat taaaaaagtt aatccaaatg
     6301 ctataagtaa ttacgaagaa gccaaacggg cagccaaacg actaaagacc aaaatgaaga
     6361 ttgttcctgt tacgactgtt caagaggcac tggtttatct tcgcaaataa aataactatg
     6421 aatgtattat attcaattat attcacgcta ttaactcata aaaaaacggt tgaaccagat
     6481 agacagatcc ttgaccagtc ttttgggttc aatcattttt tacataaaac atatctaact
     6541 tgacagcgta aaaaagatgt tataaaagtc tagtggtaaa aaggcaatag agaattgagt
     6601 aaactttagt cgattatctg gaaatagagc atactaaaaa cacctagtga aaattgattt
     6661 ttttcactag ctcatccaag aacttgcgca gataatcatg agtccattcc atgactctat
     6721 tattctggaa agccttgcaa tagacaagct aacgttataa caactaaatt aaaaacttga
     6781 gattgaaaaa tccccataaa cttattttac aaggaggtaa ggctatggta accattaaac
     6841 aagttgctga agaagcaggt gtttctagat caacagtctc caggtatatt tctcaaaaag
     6901 gatatgtatc tgatgatgca aggcataaaa tcaaagcagc gatagcaaaa ttacattaca
     6961 ctccaaatgt cttggcgcag tcattgaaga cgaaaaaaaa tcaactagtc ggtttattat
     7021 taccagacat ttccaatcct ttttttccga ggttagcaag aggagcagaa gaatatttaa
     7081 aagaaaaggg gtatcgggtt atgctaggaa atatttcaga cagcgaagct cttgaagaag
     7141 aatacgtcca tgtccttctt caaagcaatg ctgctggcat tattactacc catgacttta
     7201 caaagcggta tcctactctt gctattccag ttgtagtggt agattgtgtt gaccaagaga
     7261 cccagtacgg tgtcttttca gataataggg cagggggctt attggcggct cagactgtct
     7321 ggcaagcagg ggcgaaagaa gttctgttaa taagaggtcc gctggataat gctgaaaaca
     7381 ttaatgagcg gtttgaagct agtttttctt accttcaaaa acaagacgtg actatgtatg
     7441 tgtgtgatag tcagaatttt gattttgaaa gtattcagct agaagctagt tacaacctca
     7501 agtgttaccc aacaatagat agtattatag caccttctga tatccatgct attgcttata
     7561 ttcatgaact gcattctcaa ggtaaaaaga ttcctcaaga tgttcaaata ataggttatg
     7621 atgacatttt aatgagtcaa tttatttatc cttctttatc gactattcat caatcctctt
     7681 atttgatggg ccgttatgcc gccgaattag tttatactat tgctagtcaa ttaactgtga
     7741 aagcaaatcg gattaaattg cctgttcact acgttgagcg agaaacaata aggaaaagat
     7801 aagtaataat attagtccag attttagcag gtatgatgaa gatttattgg gatttacctt
     7861 ctactttaaa agatgttatc tgtcattact ttcatcgcta tcagtcaaaa cgcattctta
     7921 tgcaactctt aaccttaagc ttaatgtctt tttgataaca tagaggaatg ttacacgatg
     7981 atgacgcaat aaagcctaaa tatagtaatt gtcgtgattt tatagctagt gagtaatgag
     8041 atagcaagtc tctcatttac ttacaaaagt caaaaaatag tgtataatgg ttgagttaca
     8101 gataacactg caaaaaagat aacaagtgtc cataattatc taagactata aaggcgatat
     8161 agacagatat aaaaaagatg acgaatcagt tatcactgga caattaaccg atttagttac
     8221 aactaatgga tttttcagaa aggtgtcaaa tgaaaaaaga aatttcgcca gaaatgtaca
     8281 actataataa atttccaggt cccaaattta ttcattttga agagcaagtt aaggctgaag
     8341 gcattgattt gttactctta gaagatgtta agaacgcttt tgatacgact agctttggtc
     8401 aacgttatac agaagttctc ctaaagtatg actatattgt tggcgattgg ggaaatgaac
     8461 agcttcgtct taaagggttt tataaggata gtgatgatat caagaaaacg aatcgcatct
     8521 cacgtttaga agattatatt aaagaatttt gcaattttgg ttgtgcttat tttgttttag
     8581 aaaatcttca tccacaagat attaaatttg aagaggagcg ccaaccaaga cgaaagaaat
     8641 cacctaaatc aaaatcaaat cgtcgcaagc caaactattc aaatcagcag cccgcaacac
     8701 ctaagagtaa atcgaagcgg gtatcaaaag aaaagcaacc tgaaaaccaa gcgtttacca
     8761 gtcaaaaacg tcgaagtaat actaagcata aagaaaagtc aaaacgtaat cagactagtc
     8821 aacttaatac caaaattagt cattttatca tcagaaagaa agataaataa tccatgaaac
     8881 catcgattta tagcttaaca cgcgatgaac tgatcgcgtg ggcagttgaa cgcggccaaa
     8941 aacagtttag agctactcaa atttgggatt ggctctataa aaaaagagtt caatcttttg
     9001 aggagatgac gaatatttct aaagattttg tttctatttt aaatgatagt ttctgtgtca
     9061 atcctttaaa acagcgagtt gtacaagagt cagctgatgg tacagtaaaa tatttatttg
     9121 aactgcccga tggtatgtta attgaaacag ttttaatgcg tcaacattat ggtcattctg
     9181 tttgtgtgac gactcaagtt ggctgtaaca ttggctgtac tttctgtgct agtggattga
     9241 tcaaaaagca acgtgatcta aacagtgggg aaattacagc tcaaatcatg ttagtccaaa
     9301 aatattttga cgatagaaag caaggtgagc gggttagcca tgttgtcgta atgggcattg
     9361 gtgaaccttt tgataattac aaaaatgtca tgtgctttct tcgcgtcatc aacgatgata
     9421 atggcttagc aattggtgcc cgtcatatta cagtctctac ctcagggtta gctcacaaga
     9481 ttcgggattt tgcaaacgag ggagtacagg ttaatttagc agtctcgttg catgcaccaa
     9541 ataatgattt acgttcaagt attatgcgag taaatcgctc ttttccgctg gaaaagctct
     9601 tttcagctat agaatactat attgaaaaaa cgaatcgtcg tgtcactttt gaatacatta
     9661 tgctaaatga agttaacgat agcattaagc aagctcaaga attagcagac ttaactaaaa
     9721 ctattcgtaa gttatcttat gttaacttga ttccgtataa tcctgtttca gaacatgatc
     9781 agtatagtag aagtcctaaa gagcgagttc tagcgtttta tgatgtgcta aagaaaaatg
     9841 gagttaactg tgtggtgcgt caagagcacg gaacagatat tgatgcagcc tgtggccagt
     9901 tacgttctaa aacgatgaaa aaagatcgcg aaaaggttac agcgacaaaa taaagcccaa
     9961 aaaatatctt tgattgacaa tgcttgatga caaaatattc ctagtcacta aaatgactag
    10021 gttttttatc tggcctaaaa atgctctaga agcacaatcc aaaataatcc aaaactaaga
    10081 taaggaataa aaggtatgcg cttgatgcct aatagtaagc aagcagtaat acctgctaaa
    10141 ctagcaagtt gaattagcca aatcaacgac gttagatcca atactaaagc taagctagca
    10201 aggtagaaaa aatcgcctgc tccaattgaa aaaggtctta aatgagctaa taaacctagt
    10261 agcagtaata tcaatgataa ataattgatc gaataaaaca ggagtagact tacaaagcta
    10321 aaaagccaca agataaaagg gtattcttga gaacgccaat caaataaact aaataataaa
    10381 gaaaagagta acaagtaaca gtaactagca ctaacccaat gtaccatact agcccataag
    10441 ataattatta gacttatctc taagctaaag aagatttttt ggctagttat aaaatggttt
    10501 tggagctgcc gaaaaacgcg tttggcaaaa gttagttggt cacaaacaat gacagtctgt
    10561 ttacttagct gttcttgacc attatcagga aaataaagta gtaaacagta aaaaagatgg
    10621 gtcaataaga tgcccgaaca tatactaaaa aaagctatca tacttattta ttcgaaaaga
    10681 aatcaaatac ttgcaatctt tccttaatca ttgtagaata tagatataaa acgataatga
    10741 ttctaattaa gtgtctaatc atccaattag gattattccg ttattgtcga ataggattag
    10801 gtccttaacc ctttcttatg tttatcacgt tagggttagt tccccctcca ttcttgccct
    10861 ttaagctgaa aagtaaggag atgttattat gacaaacaca ctcgttgaaa atatttatgc
    10921 ttcagtaact cacaatattt ctaaaaaaga agcatcaaaa aatgaaaaaa ctaaagctgt
    10981 tttaaaccaa gctgtcgctg acttatctgt ggctgcatct attgtgcacc aagtacactg
    11041 gtatatgcgt ggtccaggtt tcttgtacct tcacccaaaa atggatgaat tacttgatag
    11101 cttgaatgct aatttagatg aaatgagcga gcgtttgatc actattggcg gagcgcctta
    11161 ttctacccta gctgagtttt ctaaacactc taaattggac gaagcaaaag gtacttacga
    11221 taaaacagtt gctcagcatt tagcaagatt ggtcgaagtt tacctttacc tcagcagcct
    11281 ttaccaagtt ggtttggata ttaccgatga agaaggcgat gctggaacaa acgatttgtt
    11341 tacagcagct aagacagagg ctgaaaaaac catttggatg ctccaagcag agcgtggaca
    11401 aggcccagca ctctaatgga ttatgcctta ggataaaaga agtcaatgat aaaattggct
    11461 tcttttttat ggaatcatcg attcattttc agaaacatag aaagcgattc catacttgtc
    11521 ttgaaatttg ccgtagaatt tgctaaaatc tacttatatg aaaacactat atgatgtgca
    11581 acaattgcta aaaaactttg gcatttttgt ttaccttggc aaacgtctct atgatattga
    11641 aatgatgaag attgaacttc agcgtctcta tgatagcggc ttattggata agagggatta
    11701 cttaaatgcg gagttaattt tgcgacgtga gcatagatta gaattggaaa aagaaggata
    11761 gacaaatgag tcaaaaatta ctggggattg atttaggtgg taccaccatt aaatttggaa
    11821 ttttaacagc agcaggagaa gtccaagaaa aatgggcaat tgagactaat attttagaag
    11881 gaggcaaaca tattgtccct gatatcatcg cttccatcaa acatcgctta gacttgtatg
    11941 gtctaagcag tgctgatttt gtgggaattg gtatgggatc accaggagcg gttgaccgtg
    12001 atactaatac agttactggg gcattcaacc ttaattggaa agaaacccaa gaagtcggtt
    12061 ccgttgttga aaaagaattg ggcattcctt ttgccattga caatgatgcc aatgtggctg
    12121 cccttggtga acgttgggta ggtgctggtg aaaataaccc agatgtcgtc ttcatgacac
    12181 ttggaacagg tgtcggtgga ggcattattg ctgatggtaa cttgattcat ggtgttgcag
    12241 gagcaggtgg tgaaatcggc cacatgattg ttgagccaga aaatggcttt gcttgtactt
    12301 gtggctcaca cggctgtttg gaaacagtag cttcagcaac aggagttgtc aaagtggcac
    12361 gtttattggc agaagcttat gaaggggatt cagccatcaa agcagctatt gacaatggtg
    12421 aaggtgttac cagcaaagat atttttatgg cggctgaagc aggggattcc tttgctgatt
    12481 ctgttgtgga aaaggttggt tactaccttg gccttgcttc agcaaatatt tccaatatct
    12541 tgaatccaga ctcagtggtt atcggtgggg gtgtgtcggc agcaggagaa tttctacgct
    12601 cacgtattga aaaatacttt gtgaccttca ctttcccaca agtgaggtat tcaactaaaa
    12661 ttaagattgc agagcttggc aatgatgctg gaattatcgg tgcagctagc cttgctcgac
    12721 aatttatcac aaaataggag ggggatatgt ctccaatcac acttatctta tggttacttc
    12781 ttgtcggcat tgttggttat tatacatgga actatttctc ttttcgaaaa atggcaaagc
    12841 aggttgataa cgagactttt aaagacgtga tgcgtcaagg tcaattgatt gatttacgag
    12901 agccagcggc ttttagagct aagcatattt taggagctcg aaattttcca gcgcaacaat
    12961 ttgatgctgc tatcaaaggt cttcgcaagg acaaacctgt tcttatttac gaaaatatgc
    13021 gtccccagta ccgtgtgcct gcggtcaaaa aacttaaaaa agctggcttt gaagacgttt
    13081 atgtcttaaa agacggtatt gattattggg atggcaaagt taaacaaaca acctagttaa
    13141 gagtaacgtg gagttaaggg gaataaaggt agtcactgtc tcaaaaacct taaatccttt
    13201 ttttgctgta tccagacttg ctgaaagtct gaaaacattt acaattgatt aaaaccagtt
    13261 ttttaaaaca ttttgtgtta tacttatcta gttaaaatat atttacttag aggaacaaat
    13321 gactaactta agaaacgata tccgtaacgt agcgattatt gcccacgttg accacggaaa
    13381 aacaacactt gtagatgaat tattaaaaca atcccatact cttgatgagc gtaaagagct
    13441 tcaagagcgt gccatggatt ccaatgacct tgaaaaagaa cgtgggatta caatccttgc
    13501 gaaaaatacg gcagtagcct ataacgatgt tcgtattaac atcatggata ccccaggaca
    13561 cgcggacttc ggtggtgaag ttgaacgtat catgaaaatg gttgacgggg ttgttcttgt
    13621 tgtggatgcc tacgaaggaa caatgcccca gacgcgtttc gtattgaaaa aagcacttga
    13681 gcaaaacctt atcccgatcg ttgtggtgaa caagattgac aaaccttcag ctcgtccagc
    13741 agaagttgta gatgaagtgc ttgaattatt catcgaactt ggtgccgatg atgagcaatt
    13801 ggaattccca gttgtttacg catcagctat taatggaaca tcatcattat cagatgaccc
    13861 tgctgaccaa gagcatacta tggcaccgat ctttgatacg attattgatc atattccagc
    13921 gccagttgat aattcagatg agcctttgca attccaagtg tcacttttgg actacaacga
    13981 tttcgtaggt cgtatcggta tcggtcgtgt tttccgtggt actgttaaag tgggtgacca
    14041 agtaactctt tcaaaacttg atggtaccac taaaaacttc cgtgttacaa aactgtttgg
    14101 tttcttcggt ttggaacgtc gtgaaattca agaagctaaa gcaggtgact tgattgctgt
    14161 ttcaggtatg gaagatatct ttgttggaga aaccattaca ccaactgact gtgtggaagc
    14221 tctgccaatt cttcgtattg atgagccaac acttcagatg actttcttgg tcaataactc
    14281 tccttttgca ggtcgtgaag gtaaatggat cacgtcacgt aaggttgaag aacgtctttt
    14341 agcagaattg caaacagacg tgtcacttcg tgttgaccca acagattcgc cagataaatg
    14401 gacggtttca gggcgtggag aattgcattt atctatcctc attgaaacca tgcgccgtga
    14461 aggctatgaa cttcaagtat cacgtccaga agttatcatc aaagaaattg atggtgtcaa
    14521 atgtgaaccg tttgagcgtg ttcaaattga tacaccagaa gaatatcagg gtgcaatcat
    14581 ccagtctctt tcagaacgta aaggggatat gcttgatatg cagatggttg gtaatggtca
    14641 aacgcgtttg attttcttga ttcctgcacg tggtttgatt ggttattcaa cagagtttct
    14701 ttcaatgaca cgtggatatg gtatcatgaa tcatactttt gatcagtatc taccggttgt
    14761 tcaaggagaa atcggtggcc gtcatcgtgg tgccttggtt tctattgaaa atgggaaagc
    14821 aacgacatat tcaatcatgc gtattgaaga acgtggaact atctttgtca acccaggtac
    14881 agaagtttac gaagggat
//
LOCUS       DAUVUY010000046        14771 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.046, whole genome shotgun sequence.
ACCESSION   DAUVUY010000046 DAUVUY010000000
VERSION     DAUVUY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14771
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.046"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(189..1595)
                     /gene="lacG"
                     /locus_tag="VOX58_001167"
     CDS             complement(189..1595)
                     /gene="lacG"
                     /locus_tag="VOX58_001167"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HES5878398.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFAALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(1668..3365)
                     /locus_tag="VOX58_001168"
     CDS             complement(1668..3365)
                     /locus_tag="VOX58_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HES5878399.1"
                     /translation="MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMQVILFSSIF
                     ILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKVYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKEQMEK"
     gene            complement(3365..3682)
                     /locus_tag="VOX58_001169"
     CDS             complement(3365..3682)
                     /locus_tag="VOX58_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HES5878400.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(3706..4689)
                     /gene="lacD"
                     /locus_tag="VOX58_001170"
     CDS             complement(3706..4689)
                     /gene="lacD"
                     /locus_tag="VOX58_001170"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HES5878401.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEAGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDVNEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKIADNASPEFAKVKAHKVNEAMKVFSEERFGVDVLKVEVPVNMKFVES
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(4692..5621)
                     /locus_tag="VOX58_001171"
     CDS             complement(4692..5621)
                     /locus_tag="VOX58_001171"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HES5878402.1"
                     /translation="MILTVTLNPAIDVSYPLNELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNDFGETVKATGCIGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPLLSVDEIDGFTHHFKYLLNDVDVVTLSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFEALKEVLQDELFEGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            complement(5667..6182)
                     /gene="lacB"
                     /locus_tag="VOX58_001172"
     CDS             complement(5667..6182)
                     /gene="lacB"
                     /locus_tag="VOX58_001172"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HES5878403.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHEIIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRAALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(6217..6645)
                     /gene="lacA"
                     /locus_tag="VOX58_001173"
     CDS             complement(6217..6645)
                     /gene="lacA"
                     /locus_tag="VOX58_001173"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HES5878404.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKYEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            7091..7864
                     /locus_tag="VOX58_001174"
     CDS             7091..7864
                     /locus_tag="VOX58_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HES5878405.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
     gene            8564..8800
                     /locus_tag="VOX58_001175"
     CDS             8564..8800
                     /locus_tag="VOX58_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878406.1"
                     /translation="MVSEAMTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKE
                     KLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            8790..9125
                     /locus_tag="VOX58_001176"
     CDS             8790..9125
                     /locus_tag="VOX58_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HES5878407.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFREVGFDADEKYGSKIIHYHSTKGYTLSKDYIVLYHIEGEENRVVIDYLLPT
                     QSDYIKLFK"
     gene            9253..9747
                     /locus_tag="VOX58_001177"
     CDS             9253..9747
                     /locus_tag="VOX58_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HES5878408.1"
                     /translation="MYLKASGNLEEFTKPTHKTYSEIFEEWYQAYQDTVEPTTASRTL
                     DLFQLHILPVMGELPINKTSPLDCQNFITDKAKTFKNIKQIKSYTEKIFDFAIKMKLL
                     KHNPMAEIIMPKRKKTRIENYWTVQELQEFLAIVLQKEPYKHYALFRLLAYSGLKERR
                     AMRS"
     gene            9710..10072
                     /locus_tag="VOX58_001178"
     CDS             9710..10072
                     /locus_tag="VOX58_001178"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HES5878409.1"
                     /translation="MAALRKGELCALKWANIDFQSETLSVNKSLGRLDGQAIEKSTKN
                     DFSVRKIKLDSETISILQEWKAISQKEKAQLAVAPLSIESDFIFTYCTREGSIEPLHT
                     DYINNILSKVIKQHSLKK"
     gene            10108..10257
                     /locus_tag="VOX58_001179"
     CDS             10108..10257
                     /locus_tag="VOX58_001179"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878410.1"
                     /translation="MIEIGVDPVNTAKRLGHASSQITLDTYSHATAVGEDKSIKQFAN
                     YLKNN"
     gene            complement(10390..10782)
                     /gene="rpsI"
                     /locus_tag="VOX58_001180"
     CDS             complement(10390..10782)
                     /gene="rpsI"
                     /locus_tag="VOX58_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HES5878411.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10803..11249)
                     /gene="rplM"
                     /locus_tag="VOX58_001181"
     CDS             complement(10803..11249)
                     /gene="rplM"
                     /locus_tag="VOX58_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HES5878412.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(11467..11673)
                     /locus_tag="VOX58_001182"
     CDS             complement(11467..11673)
                     /locus_tag="VOX58_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5878413.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEVDDSH"
     gene            complement(11670..12368)
                     /locus_tag="VOX58_001183"
     CDS             complement(11670..12368)
                     /locus_tag="VOX58_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878414.1"
                     /translation="MFDSKQNLAKSQWGFIVITAVMDLVVIVATLLAKTSFQRYLVGG
                     VAVILTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPRYDERQKQILLKGYT
                     IGFWFMIVAVWLSIFLHRFSERLVSASFLFTLALWGGLAVQTTYCNLNGVSPFVDRRF
                     GKQGILVGMIATGMSLITIGLAVREMMAGQLKMGSFFKAGGSGSLMVLGITLLSMGAS
                     IFYRHYLDAKEADE"
     gene            complement(12504..13364)
                     /locus_tag="VOX58_001184"
     CDS             complement(12504..13364)
                     /locus_tag="VOX58_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HES5878415.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHARNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(13461..13979)
                     /locus_tag="VOX58_001185"
     CDS             complement(13461..13979)
                     /locus_tag="VOX58_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HES5878416.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKINQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     IQLKDFMSQLDR"
     gene            complement(13983..14729)
                     /gene="rlmB"
                     /locus_tag="VOX58_001186"
     CDS             complement(13983..14729)
                     /gene="rlmB"
                     /locus_tag="VOX58_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HES5878417.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKARGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
BASE COUNT         4596 a         2971 c         2533 g         4671 t
ORIGIN      
        1 ttaaataaaa ctttcaaaac ccattttcat caactaaaaa cacttataac ccgatcttac
       61 aactaatgga ttaccatcaa cagcaaataa tactatgctt gaaaaatgtc aacatcatct
      121 actttttgtg actataattc agtaaacgaa catgaagtgt aaaaatgtat taaaaaagta
      181 tctcaaaatc attcaatcac ttgagtttct gctacttttt tataccagta ggcactcttc
      241 ttaggataac gttcttgagt ttcaaaatcg acatagaaga gaccgtaacg tttttcatag
      301 ccatttgacc atgaaaagac atccatcagt gaccacataa agtatccttt aacatttgca
      361 ccatcagaaa tagcatcaga aataacttct aagtgttttt tcacataatc gatacgtcca
      421 tcatcataga cagtattatc tacaaactca tctttgtagc caagaccatt ctctgtaatg
      481 taaattttct tgtagttagg ataatcggct ttgacacgca tgatttgatc atataagcct
      541 tgtgggaaga taatccagtc ccagtccgtt tttggaacat caacgggtgc ttttcttcga
      601 ccaacaccct tgatttggta tttagagctg cctttttccc ctttaccatt atgaatgatt
      661 tcagtctcac catcaaaagc ttgcatccaa tcactcatat aatagttaat accaaggaag
      721 tcattcaaat cttttgcagc gtctagtgca gcaaaatctt cctcacgaag atcaagttca
      781 ccaccattta cctcaaggat atggttaaca ccttccattg ttttatctga atacttacct
      841 agataagttg catcaagaat aaatttatta tggatgatat cttccagttc agctgctcta
      901 acatcatcag gattgttagc gtcaaatgga tacttagttg gaagagcatg gaccacgccg
      961 atttcacctg aatagccact atctttaaag agtttgactg cacgagcatg agagaccatc
     1021 atgttatggt gtgattggaa aactttagca agatcatatt ggatacctgg agggaattta
     1081 ccaaccaagt attggccatc accaataggc ccaatttcgt taaatgttgt ccaatagtta
     1141 acttctgaga attctttaaa acaaaattct gcataattta caaaatgttc aatgttctca
     1201 cgattgagga aatcaccatc cgagtggaga gcttctgggg tatcaaaatg gtgaagtgta
     1261 acaaaaggct caacatgacg cttatgacac tctgcaaaga gattgtggta gtattcaact
     1321 cctttagggt taacttctcc ttttcctgtt ggaaaaatac gagaccaggc aatagagata
     1381 cggatgccgt tgacaccaaa ttcttcacta agtttcaaat cgactggata acgattgtaa
     1441 aaatcacttg ctggctcagc tgtgtaccaa tagttgtctt ctaagtattt atcccaagct
     1501 actggtcctt taccatctgt gtgggtagcg ccttcagcct ggtaagcagc tgtagcacca
     1561 ccaaaaataa aatctttagg taatgtttta gtcatgatat acctcttcta aattttcatt
     1621 tcactataaa ggaagattga gtacccccaa ccttccctag cgacatatta tttttccatt
     1681 tgttctttaa cgaagtctaa tgcgccttga ccatcgcgag tcaatttgat gtattgagcc
     1741 ccttctgttt tagctaattt aatatcaagt ttatcagttt ccactttcat atcttcatag
     1801 ttagaagcta cttgaggtgc aaggataaca agttgatatt gtggtaaaat ctcacggtga
     1861 gcaccataac ttcctgctgc tgcagtgaca ggtgcaccat attctttagc tgctttattc
     1921 aaagcgtttg caagcaaacc acttgtacca ccaccggcac aaagaacgag aacattagtt
     1981 tgatcagtga tttctttggt ttctttagct gctgattttt caagaatagc atcagctttt
     2041 ttagtatcaa agtttgctgc tactttttct ttgagggcat ctgatgaatt cgtacctgct
     2101 gcttcttcag caagaatttg ctcatcatat acttttaaga atgggtagta gataatcaca
     2161 tcaacaacaa ttaacaaagc tgccaaaatg aatgctagca cttggaagtt tgtcccaagg
     2221 ataagaccca atggagctgg tgttgtccaa ggtaagtttg cagtgaaact gttcattcca
     2281 agtgtgtcaa tgaagaattt gaaaatccat acgttgacaa tcggtgccag gataaatgga
     2341 ataaagaaga ttgggttcaa aacaagtggg gcaccaaaca aaatgggttc gttaacacca
     2401 aagaatgttg gaacaactga tgcacgtcca atggctttgt tacgttttga ttttgttaac
     2461 cacatgaaca tgaatggaac gacgagagtt gcaccagtac cacccatggt aacgatgaac
     2521 atttgagtcc ctgaagtcaa gatcttatca gcatgttcac cagcttgaag taattgaagg
     2581 ttggtttcag catttgcgta agtaatcgct gcaatggctg gttcaacaat agatggtcca
     2641 tgaataccaa caaaccagaa gaaagcaaat gcaccgaaga taatagtgat tccaacatag
     2701 ccatctgctg cttggaacaa tggtgcaaga agtttaccaa ttgattctgc aacgttaacg
     2761 cctaaagtag tatgaacaat caattcaatc acataaagca gtacaactga taacgtaaat
     2821 ggaatcaaat ctttaaaaac ttgagatata tttggtggaa cctcttctgg catgcgaata
     2881 gtaacattgt ttttcacaca ggttttatag acatttacgg taataaaagc tgcgatgaag
     2941 gctgtcaaca aaccttttgt tcccataaat ccagttaaga aaccaccgtc ttttgctgga
     3001 ttggctgcca taagtaaaaa acctacaata gcagcgagca tagttgaaat aaagttgatt
     3061 tggtttgtgc ttggtaatga tcgattcatt gagtcagtta aagctttggc tgtcgtacca
     3121 gccacaaaca aacctaaaat acccattgag tagttgtatg gcgtcatcaa gagattctca
     3181 atatccttac tccaatggaa gccccatgca tttggaacat aggcaatcaa gataaagata
     3241 cttgaaaaca agataacttg cataccagcg ataaaaccat cacgaatagc gcgaagatag
     3301 atatttcgag aaattttctc gaagaaaggt ttccctttct caatctgagc aattaatcca
     3361 ttcattattt tgctcctctc ttataaagtt caatcatgtg tttcattaaa tcttgtaata
     3421 aaatggtcgt cattaaatga tcttgaccat gcattaaagt gacactataa gctaagtcat
     3481 ctccagctgc ttctttttgt agtagacttg tctgagcatt gtgagcatca ttcaagcagt
     3541 cactacctgc agcgattaat tcttctgctt tagcataatc tccttcttgc gctgctttaa
     3601 gcgcttccaa aaattttgaa cgggcatcac cagcataggc tacgatttca aaacctaaaa
     3661 gagtagtttc ttctctattc attgtagttt cctccgaaaa taatgttaca ttttttcagt
     3721 ccaaggactt gcagttttat ctagtacttt gtttaattcg tcaatatttt tgaaaccttc
     3781 tgtacgcaac cattcacgag ctgcttgtgg tccttcttcg atatagactt ttacagatcc
     3841 tgcccaggta gcacgtccac ataagacacc gttgaattta gcacctgatt ctgctgcaaa
     3901 caccaaagta tcttggaaca atttggcaga aacacctgca ctcaagtaaa tatatggtaa
     3961 atcagtagaa gcttcttggt cacggaaagc ttgagctgct tcttcttttg taaagagaac
     4021 ctcaccatct gcaaagcttt ctacaaattt catatttact ggaacttcga ctttcaagac
     4081 gtcaacacca aagcgttctt ctgagaagac tttcattgct tcattaactt tatgagcttt
     4141 aacttttgca aactcagggc ttgcattgtc agcaattttc tcatcataag ttagaatttc
     4201 taagtagaat ggaatatctt ctgcacggca ttcagaacca atacgttcaa tgtaagcttt
     4261 tttctgctcg ttaacatctt gatctccatc aatatcatag taaagaagga atttaactgc
     4321 ttctgctcca gcttctttaa tacgttttgc agaccaaaca tctaagcaat caggtaagcg
     4381 actagttgtt gttgcatcgt atcctgtttt ctcatatgcc aacaacaagc ctgcttcttc
     4441 tgaacgaaca cgacttgctg gcaagccata ttcaggatca agtaaaattg atgatgcaaa
     4501 tggtgtcagt tcttcagaaa ctaaactttt aagctcttca atttgttcta ctgttggctc
     4561 ttttgtttga tgttgagcca tcatacgttt gagagctcca cgttggtcaa aagctaaagc
     4621 agaaataaca ccatctatac ttagtttttc catgctttta cgtttgtttt cagttaaagt
     4681 aattgtcatt tttatacctc tttgacttta atagattgat ataacttatc gtaatttgcc
     4741 atgttaacat gcccagttgt tttttcttgc gcatttaaca tacctaagac atttgctttt
     4801 gttaataaag ctcgatcatc ttcatcattc gctaaacctg aagcaatacc tgccactgta
     4861 gaatcacccg aacctacagc gcttacaatt ttgattttag gaatgtcaac gttataaaac
     4921 gtatccttgt gctttgcaaa tacaccatca gcaccaagag aaacgattat ccattcaatt
     4981 ccttcaaata gttcatcttg taagacttct tttaaggctt caaaatcttt tgtcatttct
     5041 ttacctagaa gttgagaaag ttcttcaaga ttaggtttga taactgttgg tttactatca
     5101 cctttcaata ctgcttctaa agcatttcca gaacaatcca gaacagtctt cttaccatta
     5161 agattggcaa tcttgattaa cttctgatag taatcatctg gcataccagc aggcaaactg
     5221 cctgagagcg tcactacgtc aacatcattc agtaaatatt taaaatgatg agtaaatcca
     5281 tcaatttcat ctacacttaa cagcggtcct ttttctaaaa tctcagtttg attaccttcg
     5341 tgtaatatgg ctatacatgt tctggtatca cctgaaattt tatagaagcg acttaaaatc
     5401 gaatcaggaa gatgattaat gataaaatca ccgctttctc caccaataca accagtagct
     5461 ttgactgtct caccaaagtc atttagaact cgggaaacat tcaagccttt accaccgggt
     5521 gtctttgtaa cgtctacaac tcgattgaca gtatcgcatt ttagctcatt caaagggtag
     5581 gagacgtcaa tagctggatt taaagtaact gttaaaatca caatagctcc tttttctata
     5641 tatccttatc ataattcttt caaatattag tcgtgatatt caccacggtc ccatttttct
     5701 agaaattcat caaagaagtg tgggttgttt tgatcttggc tagcatgttg caagctatcc
     5761 attttttcaa tccatttttt attttcttca gttggtttgt attcagccgc taagaaagca
     5821 tcgataatat cattcatcag caaaccaccg atgatttttc caccaaaacc aataacgttt
     5881 gcgtttagct cttcttttga gtagatagca gatgtcatat ctctaacgag tgctgcacga
     5941 ataccgggaa ctttgtttac agcatttgtg ataccaacac cagtaccaca gataacaaca
     6001 cctaattctg cttcaccact tgctactgct tcaccaactt ttttaccaaa gattggatag
     6061 tgtgtgcgaa cattatcgta tgtaccgcaa tcaatgattt catgaccttg tgattttagg
     6121 taatccacaa cagcgatttt ttcataagta acaatatggt cacaacctac agcaattttc
     6181 atttttgttc tccttgtttt aacctatttc aatcaattag cacattttat taagcatgtc
     6241 gacacgaact tgatgacgtc caccatcata tttaccttcg acaaagcctt tgataatgtt
     6301 ctttgcaatt cctggagcac taatttcaga accaagcgtg atgattcgag cattgttgtg
     6361 tcctcttgtc atgtatgctg aacgctcatc tgatacttca gctgccacca ttcctttgat
     6421 tttagtagct accatgaaag aaccaacacc atatgcatca atgacaatac ctaagttatc
     6481 ttcggaggca ttaacttctt ttgcaacggc taaagttgta tctacaaagt cacgtacttc
     6541 gttttcacta acatcaacga cttcgtattt accttcttta aggtaatctt tgataacttc
     6601 tttaagttct tgacctgctt tgtcagcacc gataataatt gccattttat ttctcctatg
     6661 atttagaata agtaaatgtg gaaaacctac gtagatagtt aattaacgat ttgcaacatc
     6721 ttttgaaata acgctttcat tatttctatg acctaatcat acacccacca aacataaaag
     6781 tcaacatttt tttgtttatt tttataaaaa ataaacaaaa tatgtttatt tttttcattt
     6841 ttacgttatt atcctaatgt ttcttgtgtt tctttttcat ttcataaatc cctttgacag
     6901 ccactcgtaa cctatcatat attacgagac ccttatctct atttattgta aacgttttat
     6961 ttaaaacatt ttctattttg ttaatttttg ttttttccaa tgtttatgtt tgttttgttt
     7021 gattttttta ttgatattat gttatgatac ctttaatagt tataccctaa gacactgata
     7081 ggagataaga atgaaaaaga aagagcgtca tgaaaaaatt ctcgatatct taaaagttga
     7141 cggttttata aaagtaaaag atattattga tgaaatgaac atctcagata tgaccgctag
     7201 acgagatctc gatacattgg cagataaagg attacttatc aggacccatg gtggtgctca
     7261 atatttggat tattcttcag caaaggacga aggtcacgag aaaacacaca ctgaaaagaa
     7321 ggtcttacag actaccgaaa aaaaacaaat tgcacaaaga gctaaaaaca ttattaaaga
     7381 cggcgaaact gtctttatag gtccagggac aacattagag catctagctg tcgaattaaa
     7441 aaatagaaat atcagggtca ttacaaatag ccttcctgtt tttttaattt tgcacccgag
     7501 cactactatt gatttgattt taattggtgg tgaatgtcgt gaaattactg gagcttttgt
     7561 tggtgctatt gccattaata atctagaatc attaactttt tcaaaggctt ttgttagcgc
     7621 aaatgcagtt tacaataatt ctattgcgac gtacagtgat ctcgaaggag aagttcaaag
     7681 ggttgcattg aataatgctg ttgagaaaat ccttctagtt gatagtacca agtttgaaag
     7741 ttatgacttc tataattttt accaactaga tcaagtggac ttaattgtga ctgataaaaa
     7801 tgtgacaact gatatcatag aaaagtacaa gaaattctca aaaatcataa tcgctgacaa
     7861 gtaaaggacc tactcatatt aaaaagcact tcagggttta gatatatccc ctgtcaagta
     7921 aacagggagc atatctgaaa gtagtgtttg tgttataaag aagtttcttg ctaacttatt
     7981 ttctggtttt tttcagactt atcagtcttg tactgttcct ttttctgatg aataacgtgg
     8041 aaataggcag acagctatat atttcacctc tagaatggtt gtcattactg tcatcaagac
     8101 atgcttgttc tttatcgtat ggagtgaagc tttcttttag acatcaacat acctcttaag
     8161 tagattttgg ttcgttacca tgtctactta agaggtgtct tcaaagcgct ttaaaacgtt
     8221 gatataacag tatattaacg ttttgagaat tgtgaagctt tacgagcttt tttaagacct
     8281 ggtttttggt tcactccatt atttaaagtc aaatcccttt aaaatcaatg tttctaaaat
     8341 gaaaaatcac taaaaatctt atttatagac aaatatttaa acctaaacgg tatcaaaaat
     8401 aagtttcgga gcttatcaaa taatgaaaat ctgtctcaat taccgagtct tttttattga
     8461 cttttttgta tatatgatat aattaaataa attacataaa ggggttacat aaaatgacta
     8521 cagtaaaaaa aacagagcgg ttacctttca agctaataaa gaattggtaa gcgaagcaat
     8581 gacagtatta aacaaaaaaa atttaacctt atcatctgct ttaagattat tcctacaaaa
     8641 tgtcgttgtc acaaacgagg ttgacttatt gacggaagag gagttagaga aagaaaaact
     8701 tttcaagcaa tttcaagctg aaatcaacaa aaatattgag gatgttcgtc aagggaagtt
     8761 ttatacctct gaagaagtga gggctgaact tggactataa gaaatatcag attatctatg
     8821 ctcctgatgt tttagagaag ctaaaagaaa ttcgtgatta tatttctcaa aactattcct
     8881 caacatcagg tcagcgtaaa atggagcaaa tcattagcga catagaaaaa ctcgaggttt
     8941 tccgagaagt tggttttgat gccgatgaaa aatatggttc aaaaatcatc cattatcaca
     9001 gcactaaagg ttatacctta agtaaagatt atattgtcct ataccatatc gagggggaag
     9061 aaaatagggt cgttattgat tacttgcttc ctactcaaag cgactatata aagttattca
     9121 aataacacat taaaagcctt agcagtcttg ctagggcttt tttggtagat taccaattaa
     9181 ctaggagcaa aataagttcg ggctgtttag tagatattcc acaaagaagc caaagactac
     9241 gaagataggc taatctacct taaggcttca ggaaacttag aggagttcac caagccaact
     9301 cataaaactt atagcgagat attcgaggaa tggtatcaag cctatcaaga tacggtagag
     9361 ccaaccacgg cttctcgtac tcttgatctg tttcaacttc acattttgcc agtcatggga
     9421 gaattgccca ttaacaaaac ctccccttta gactgccaga acttcatcac cgataaagca
     9481 aagaccttta agaatatcaa gcaaatcaag tcctacactg aaaagatctt tgattttgcc
     9541 atcaagatga aactgctcaa gcataaccct atggctgaaa tcattatgcc aaagcggaaa
     9601 aagacacgaa tagaaaacta ttggacggtt caagaacttc aggagttttt agccatagta
     9661 cttcagaaag agccttacaa gcactacgca ctatttcggc tactagctta tagcggcctt
     9721 aaggaaaggc gagctatgcg ctcttaaatg ggctaatata gactttcaga gtgagacact
     9781 aagcgtcaat aagagcctag gacgattaga cggacaggca atcgaaaaaa gcactaaaaa
     9841 tgatttctca gtaagaaaga ttaagctaga cagcgaaact atttctattc tgcaagaatg
     9901 gaaagccatc agccaaaaag aaaaggcaca actagcagtt gcacctcttt caattgagtc
     9961 tgactttata tttacatact gtactagaga agggagtatt gaaccacttc atacagacta
    10021 tattaacaat atcttatcaa aggtcattaa gcaacatagt cttaaaaaat aagtcctcac
    10081 ggcttcaggc atacccatgc aaccctgatg attgagattg gagttgaccc agtaaataca
    10141 gccaaaaggt taggacatgc tagtagtcaa ataactcttg atacatacag tcatgctact
    10201 gcagtcggag aagacaaatc aatcaaacaa tttgctaact atctcaaaaa taactaaata
    10261 aacgtattaa accaagtcaa aagttttccg aaacggtatc aaaaaaaggt atcaaaagca
    10321 aaaaacgaca aaaaaagcac cttgcaagag acttgcaaaa tgcttgtaat cgttgatgta
    10381 aagctgttct taacgttttg agaattgtga agctttacga gctttcttaa gacctggttt
    10441 tttacgttca accatacgtg cgtcacgtgt aagaaggcca gcgcgtttca acgaatcgcg
    10501 gaagtctggg tctacttgaa gaagcgcacg agcgatacca tgacggatcg cacctgattg
    10561 tccaccgtaa ccaccaccaa caacgttaac gaaaacgtcg tatgaacctt cagttgatgt
    10621 aactgcaaaa ggttggttaa tgatcaaacg taggtcagca tgtgggatgt attcttctac
    10681 atcttttttg ttaacagtga ttttaccagt acctggaacc aaacgaacgc gtgcaacagc
    10741 gtttttacga cggccagtac ctgcatattg tgcttgtgcc attatttaat gctcctttcc
    10801 tcttagataa gtcctgagat gtcaagtact tctggttgtt gtgctgcgtg agtgtgctca
    10861 ccgccaacga agactttcaa tttcatacct tgtgcacgtc caagagtgtt atgtggaagc
    10921 atgcctttaa ctgatttttc aatcaagcga actgcgtttt tagagcgaag ttcaccagca
    10981 gtgattgatt tcaaaccacc tgggtacatt gagtgagtgt aataaacttt atcagtcgct
    11041 tttttaccag ttaatttaac tttttcagcg ttgataacga taacaaaatc acctgtatca
    11101 gtatgtggtg tgaaagttgg tttgtttttt ccgcgaagta cgctagcaac tactgcagaa
    11161 agacgtccaa gtggcacatc agttgcgtca acaacgtacc atttgcgttc aacttggcct
    11221 ggtttagcca tgaaagttgt tttgttcatg atttctccta tacgaattcg taatatttgt
    11281 ttacaaggtg atgggtgttc catcccatcg aaggtatttg gaaggttccg gggcctttca
    11341 aatggggtaa acaataccgc ctactatgat atcaaactta agaggctcca gtcaagtgct
    11401 ttagctgatt ttattggatt attttttctt tcgtttttga ggaatggcag agctgctctg
    11461 ctgatgttaa tgggaatcat caacctccca aaaaaggtca tctaaggtct tatcaagtac
    11521 tcgacaaatg gcaatgcaaa gattaatcgt tgggttgtaa tcaccttttt caacagccgc
    11581 aatggtttgt ctggagacgc ctaccaaatc tgctagagct tgttgagaca aatctttgcc
    11641 tgcacgcgct gcttttaatt tgaggttctt cattcatctg cctctttagc atccagataa
    11701 tgacgataga aaatactagc tcccatacta aggagcgtta tgcccaacac cattaaagat
    11761 ccactgccgc cagccttgaa aaagcttccc attttaagtt gccctgccat catctctctt
    11821 accgccaaac ctatagtgat taaactcatt cctgttgcta tcatccccac cagtatgcct
    11881 tgttttccaa agcgcctatc cacaaatggc gataccccgt tgaggttaca atatgtcgtt
    11941 tgaactgcca accctcccca aagagccaat gtaaagagga agcttgcaga taccagtctc
    12001 tctgagaagc gatgaagaaa gattgacagc caaacagcca caatcataaa ccaaaaccca
    12061 atagtgtatc ctttgagaag aatctgtttt tggcgttcgt catatctggg cagcttctga
    12121 tcgtccctta ccttagaatc tagtacttct ttgctatcta gtctcgtttg gagctttttc
    12181 gctgtcttta gcccccatac caacaatatg agaaagctag tcaatataac cgctacgcct
    12241 ccaactaagt aacgttgaaa actggtctta gccagtaatg ttgctactat cacaactaga
    12301 tccatcactg ctgttatgac aatgaaaccc cattgtgact tagcgagatt ttgtttgcta
    12361 tcaaacatga ttcgtttctc ctttaaatct ttatatgaca taatataaat tattttggat
    12421 attttgtcaa atatatttta cattcttgat tgatttaagc agcaaaaaag gattgaggat
    12481 ttctcttcaa tccttataag ggattaacga gaattctgcc caatcacaaa aactgccagg
    12541 gtgttaggac caacgtgagc tgagataact ggtcctagtg gcatcataag aacatcgcta
    12601 atattctcat gcgccagcag ctcttctcgt aacttttcgg cactaccctg atcgcttgta
    12661 taagagacaa taaccgtcga atccgcaata tctttttcca cttgagccac catttctttg
    12721 atggcttttt ggcgtccccg aatttttgca atgggcacca atttcccttc ctcatcaatc
    12781 cacaaaagag gtttgatact ggctaaactg cctagaaaag ctgaaccttt tgacagacgc
    12841 ccaccacgca tcaaatgaaa aagatcatct actaagaaat aggtgcgcag tcgaggaatc
    12901 actgcttcaa caatatcttt agtctccagt aaatttttgc cactatctct ggcttctgct
    12961 gctaaaatgg ttagatagcc ttctcctcct gcagcagcca aggtatcaac aatttcaatc
    13021 actgcatcgg gataatcttc tcgaacaaga tcgcgtgcca ttagagcgct ttgataggta
    13081 cccgataaaa ccgaagaaaa agcaagataa agcagtgctt tgttgttcct agcatgttca
    13141 cgaaaaacct tctcaaactc tcccacatta atctgactgg tttgaggatg actgccagct
    13201 ttcatctttt tcaaaaggta atcactacta atacggttag gaccgaccgt ttcataaacc
    13261 tctccatcgc acaaaatcgt taatcctata aggacaatat catgatcttc tgcccaggtt
    13321 tgattcaaat cagcggttga atctgtcatt attgtaaagg tcattcttac ttctcctcat
    13381 ccagtttttt agtttgataa tcaaataatt attgttttat tttatcaaaa aaacccgcac
    13441 ttggcgagtc gtaattctca ttatcgatct aactgagaca taaaatcctt caattgaata
    13501 agctgtcctt ggtcaaaagg ccgcagttta ggcgttttga gatcaatatc tctagacatt
    13561 ttatctaaat cagctttgac agtatgaact ctttgctcta attctcttgc agtgacacga
    13621 agagctcctt gagaaaaaat cgtccattgt tcatttaaat cactcgtagc tacttctacc
    13681 atatgtatag ccgtatttaa ctctgctgcc atgcgctcaa tgtagctatc tgctgtttcg
    13741 tcttcctctg taaataccac tgagatataa tactggtcat atcgttgcct taaccccggt
    13801 acatattggg catcaaaaac acaaataata ttaatattct caaaatgggc ataatgatta
    13861 agttttgtta aaagtgtgtt acgtgcctga tcaagctgat ttatcttaaa caactgacgg
    13921 gttgattgcc aaaaggcaat catattatac ccatctacta ataatatccg ttttttcata
    13981 atttagcgcc tatttcggaa aacttcatac atgagaatag ctgccgctac gctagcattt
    14041 aagctctgta cgtgaccatt cataggaatc gtaatcattt catcaacttg ctttttgata
    14101 ttggtagaga tgcctttgcc ttcgttacca atgactaaag caagtttacc attagtgttc
    14161 cagcaatcag acggtgttcc attcatgtct gtgccaaaaa tccagaatcc tcttgctttc
    14221 aatttatcta gagtttgact aaggttagtt actctcgcta tcggaatatg ttcaacagca
    14281 cctgtagacg tctttgagac cactggagtc acaccaacag aacgatgttt aggaataatc
    14341 actccacata catttgtagc atcagctgtc cgtaaaatcg atcctaaatt atgaggatct
    14401 gtcagaccat ctaaaataag aatcaaagga tttgcttctt gctctgctat ttcgaggatc
    14461 tcatcaacat cagtataggc aaaagctgat actctcagaa caaaaccttg atgcacagct
    14521 ccatcagtca tttgtgacaa ggttttttta ggcgtccatg aaatagcgac ctttttttgt
    14581 gtcgctaagc ttttgatgtt atccactttc tttcctctta aatcctcttg gatataaagc
    14641 ttatttcctg tatttgcttg aaggctttct gtaacggcat gaacaccata gacgatatcg
    14701 tttgtttcaa tagtatcttt atcttccatg agtttattat accatgaatg cttcaaaata
    14761 aatgcataga c
//
LOCUS       DAUVUY010000047        14687 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.047, whole genome shotgun sequence.
ACCESSION   DAUVUY010000047 DAUVUY010000000
VERSION     DAUVUY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14687
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.047"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1020
                     /locus_tag="VOX58_001187"
     CDS             <1..1020
                     /locus_tag="VOX58_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HES5878418.1"
                     /translation="SIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVKNALISYGI
                     DDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYLEKSLYESL
                     FPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNINGVVDATP
                     EITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLNKSNFVLLA
                     GIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEAVILTLLGG
                     VIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIKASKLDPIE
                     ALRFE"
     gene            1114..1767
                     /locus_tag="VOX58_001188"
     CDS             1114..1767
                     /locus_tag="VOX58_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5878419.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            1764..3152
                     /locus_tag="VOX58_001189"
     CDS             1764..3152
                     /locus_tag="VOX58_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HES5878420.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLM
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            3254..4819
                     /locus_tag="VOX58_001190"
     CDS             3254..4819
                     /locus_tag="VOX58_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HES5878421.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNTSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKSSHTHLTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEPNRDAP
                     KPIQPPLAAAPVFAPWRESDKDLSKLKPSSRSSAAYVRHWTGDSAYTHNLLSRRYGIT
                     AEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVRAIVAIAMAESSLGTQGVAKEK
                     GANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIANKNQTFERQDLKAKKWSLGQLD
                     TLIDGGVYFTDTSGSGQRRADIMIKLDQWIDDHGSTPEIPEHLKITSGTQFSEVPVGY
                     KRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVDRYMGNGGDWQRKPGFVTTHKP
                     KVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILISESNVMGLGTISYRTFTAEQAR
                     LLTYVVGDKLPRP"
     gene            4897..5148
                     /locus_tag="VOX58_001191"
     CDS             4897..5148
                     /locus_tag="VOX58_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878422.1"
                     /translation="MIKKVTTPSQKIKKRVRNGYLLKLGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
     gene            5824..7431
                     /locus_tag="VOX58_001192"
     CDS             5824..7431
                     /locus_tag="VOX58_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5878423.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKNCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLE
                     IGCAFEIINQDPISGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQKEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIEK
                     QYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSPS
                     KSHSDKEVQKPEKPDNSVKQATSATSS"
     gene            7621..8775
                     /locus_tag="VOX58_001193"
     CDS             7621..8775
                     /locus_tag="VOX58_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HES5878424.1"
                     /translation="MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLANTTDVKAETE
                     HVDVVLSAKEANKVFEERKALEKQARDLGDTINHMSQTISEQSRKIAALKSEAELKNQ
                     QALEALNNKNEQIAKLTNENAQLKEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAK
                     NAEIEALKQQDASKTEEIAKLQSEAATLENLLGSAKRELTDLQAKLDTANTEKEKLQS
                     QAATLENLLGSAKRELTDLQAKLDTANTEKEKLQSQAAALEKQLEATKKELADLQAKL
                     AATNQEKEKLEAEAKALKEQLAKQAEELAKLKADKASGAQKPDTKPGNKEVPTRPSQT
                     RTNTNKAPMAQTKRQLPSTGEETTNPFFTAAAATVIASAGVLALKRKEEN"
     gene            9000..10076
                     /locus_tag="VOX58_001194"
     CDS             9000..10076
                     /locus_tag="VOX58_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HES5878425.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFANQTEVKAAES
                     PKSTETSANGADKLADAYNTLLTEHEKLRDEYYTLIDAKEEEPRYKALRGENQDLREK
                     EGKYQDKIKKLEEKEKNLEKKSEDVERHYLKKLDQEHKEQEERQKNLEELERQSQREI
                     DKRYQEQLQKQQQLETEKQISEASRKSLSRDLEASRAAKKDLEAEHQKLKEEKQISDA
                     SRQGLSRDLEASREAKKKVEADLAEANSKLQALEKLNKELEEGKKLSEKEKAELQARL
                     EAEAKALKEQLAKQAEELAKLKGNQTPNAKVAPQANRSRSAMTQQKRTLPSTGEAANP
                     FFTAAAATVMVSAGMLALKRKEEN"
     gene            10287..11219
                     /locus_tag="VOX58_001195"
                     /pseudo
     CDS             10287..11219
                     /locus_tag="VOX58_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            11554..>14687
                     /gene="scpA/B"
                     /locus_tag="VOX58_001196"
     CDS             11554..>14687
                     /gene="scpA/B"
                     /locus_tag="VOX58_001196"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HES5878426.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPTAVSEEAPSSSKETNPPQIPDDAEETVADKANDPAPQAPAKTADTPATSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTDDHQDKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     PQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPKKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPIYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGGTVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAP"
BASE COUNT         5316 a         2875 c         2882 g         3614 t
ORIGIN      
        1 agtattgacc ctaaatttcc agacaagtca aatgctaaaa aaccagacta tcttcctttt
       61 atggctgaag aagagcttag caaaatacag caagtcaaag gtgtcaaaaa cgccctgatc
      121 tcatatggta ttgatgacaa agtctaccat ctaggccaaa aatcatctgc aaagatttct
      181 gccatcacaa aaaacgtagc tgaagtgagg cgcatgacat ttattaaagg cagtgacttt
      241 tcagacaaag actttattga ccaaaaacag gtgatttacc ttgaaaaaag cttgtatgag
      301 tcactatttc caaaagacga tggccttgga aaatttgtcg aagtgatggg aaatcccttc
      361 agggtcattg gggtgtttga atcaaaagaa caaagtggcc tcacaagtgg gactgagaaa
      421 attgcttata tcccgctaca tcagtggtat aatattaatg gtgtagtgga tgcgacacct
      481 gagatcacga tccagaccta tcgcgcagat gacctcaaac ctgtagcaaa gcgtgtcagt
      541 gatatgctca atcaaacgat ccctaaatca gattatatgt ttggtgtcat gaaccttaaa
      601 gaatttgagc gccaactaga caacctaaac aaatctaatt ttgtgttatt agctggtatt
      661 gctagcatct ctcttattgt aggtggtatt ggtgtgatga acattatgct agtatcagtt
      721 accgaaagaa cgagagaaat tgggatcaaa aaagcgcttg gcgctagacg taagcttatt
      781 ttaaaacaat ttttaattga agcggtcatt ctaaccttgc taggtggtgt gataggggtt
      841 atttcaggga tggtatcagg attgatcatc acaagatcac tcgagtatcc ctatatccta
      901 tccttgttct cagttgtctt gagtttagcg ttttgttgta ttatagggat tgtctttgga
      961 ctattaccag ccattaaagc gtctaagcta gacccaattg aagcattgcg atttgaataa
     1021 tcaaccaaac aagcataaaa gtcaaaaagg atgacccttt gatgtcatcc ttagctgctt
     1081 gatacactaa acgccaatct agaaagggaa gttatgttta aaatattagt agttgaagat
     1141 gatgatacca tcagtcaagt gatttgtgag tttttaaaag cgaataacta tgatccagat
     1201 tgtgtatttg atggccaagc tgccttagac aagtggcaga caacgtctta tgatctgatt
     1261 attttagaca tcatgctccc ttccttaagt ggcttagaag tgctaaaaac aattcgaaag
     1321 acatcagatg tccctattat tatgctaacg gcattagatg acgaatacac acagcttgta
     1381 agcttcaacc atttgattag tgattatgtg accaaaccat tttcaccact cattttaatc
     1441 aaacgtatcg aaaatgtatt acgcgtctct actccagatg aaaagcgcca aatcggagac
     1501 ttacttgttg atgagactga acatagcgtc tattggcaag gaacattggt caaactaacc
     1561 aaaaaagaat atgacattat tgattattta gcaaaacgtc accaaaaaat tgtcaccaga
     1621 gaccaattga tggatgacat atggggctat tctgaactgg atacgcgtgt ccttgacaac
     1681 cacattaaga accttcgtaa aaaaatgacg ggtattcccc tcaagacaat tacaggcatg
     1741 ggctatttat tgggagagcg tgagtgagac taatcaaaaa aacctttttg gtcatcaatg
     1801 gcttaattat tgtagtggtt acctctattt tgttagtgct ttattttgca atgccaattt
     1861 attacaccaa agtcaaagac aaagaagtta agcgtgaatt tgaccaaaca agcaagcaaa
     1921 tcaaaggcaa aacggtaacc gagattagag atatcctaac aaaaaaaatc aacaaagata
     1981 atatttggta cagcttagta gacagtgata accaactgct ctatccttcc ttgcagctgt
     2041 tagacggcgt cagtgaaagc aaagacagtc aaaatgttaa tatcgtcacc acctttgaca
     2101 actcttattc taatgttaag gtgatgagtc aaaaagtaac cctacgcgat ggaaaaaaaa
     2161 tgacattgct tgggcaatct tcacttcaac ccgtaacaga tgctagcaaa gttctccttg
     2221 acctctaccc gtcactattg atgttttcag tgaccgtagg gagtattgtc gcctacttat
     2281 atagtaggac ttctagccgg cgtatcctta gcatgtcaca aaccgccaaa aaaatggtca
     2341 acctagaacc aaacttaacg tgtaccattc acggcaaaga tgaaatagca atgttggcta
     2401 gtgatattaa ccgtttgtat gcgagtcttt caacgagtat caaatccttg caaaaagagt
     2461 atgaaaaagc ttctgattca gaaagagaga aatctgagtt tttacgcatg acatcgcacg
     2521 agctaaagac accaatcacc agtgttatcg gaatgattga tggaatgctc tataatgttg
     2581 gtgactttgc agatcgtgac aaatatttgc gaaaatgccg agacgtcctt gaagggcaag
     2641 cccaactggt tcaatccatt ttatctttat ctaagattga aaccctagct tctcaaaatc
     2701 aagaactgtt ttcactaaaa agcagcctag aagaagaaat ggaagtcttt ctcgtcttat
     2761 cagaactaaa acatctcaaa gtgactatca atctcgaaga acaattcgtc aaagccaata
     2821 aagtatacct actaaaggcg attaaaaata ttattgacaa tgcctttcac tataccaaat
     2881 caggcggcca agtgatgatt caactaaaag acaaccaact agtgattaaa aatgaagcag
     2941 agacattatt gacacaacag cagatgaaac agttattcca accgttttat cgaccagatt
     3001 atagtcgtaa caggaaagac ggtggtacag gattggggct gtttatcacc caccagattc
     3061 ttgatcagca tcatctggct tatcgctttg ttgttcttga tcaaagatgg atggtattta
     3121 cgattgattt tccatcccat catgacgatt gatccacact agtaggacga caaaagtcat
     3181 caatcactaa ccaagccttg acctttaccc atcattgttt aggcaagtat aaaaaagagg
     3241 agagaaatca tatatgaaga aaaggaaatt gttagcagta acactattaa gtaccatact
     3301 cttaaacagt gcagtgccat tagttgttgc tgacacctcc ttgcgtaata acacatcatc
     3361 cactgatcag cctactacag cagatactga tacggatgac gagagtgaaa cagcaaaaaa
     3421 agacaaaaaa agcaaggaaa cagcgtcgca gcacgacacc caaaaagacc ataagtcatc
     3481 acacactcac ctaacccccc cttcaaatga tactaagcag accgatcagg catcatctga
     3541 agctactgac aaaccaaata aagacaaaaa cgacaccaag caaccagaca gcagtgatca
     3601 atccacccca tctcccaaag accagtcgtc tcaaaaagag tcacaaaaca aagatggccg
     3661 acctacccca tcacctgatc agcaaaaaga tcagacacct gataaaacac cagaaccaaa
     3721 tcgtgacgct ccaaaaccca tccaacctcc tttagcagca gctcctgtct ttgcaccgtg
     3781 gagagaaagt gacaaagacc tgagcaagct aaaaccaagc agtcgctcat cagcggctta
     3841 cgtgagacac tggacaggtg actctgccta cactcacaac ctgttgtcac gccgttatgg
     3901 gattactgct gaacagctag atggtttttt gaacagtcta ggtattcact atgataaaga
     3961 acgcttaaac gggaagcgtt tattagaatg ggaaaaacta acaggactag acgttcgagc
     4021 tatcgtagct attgcaatgg cagaaagctc actaggtact cagggagttg ctaaagaaaa
     4081 aggagccaat atgtttggtt atggcgcctt tgacttcaac ccaaacaatg ccaaaaaata
     4141 cagcgatgag gttgctattc gtcatatggt agaagacacc atcattgcca acaaaaacca
     4201 aacctttgaa agacaagacc tcaaagcaaa aaaatggtca ctaggccaat tggacacctt
     4261 gattgatggt ggggtttact ttacagatac aagtggcagt gggcaaagac gagcagatat
     4321 catgatcaaa ctcgaccaat ggatagatga tcatggaagc acacctgaga ttccagaaca
     4381 tctcaagata acttccggga cacaatttag cgaagtgcct gtaggttata aaagaagtca
     4441 gccacaaaac gttttgacct acaagtcaga gacctacagc tttggccaat gcacttggta
     4501 cgcctataat cgtgtcaaag agttaggtta tcaagtcgac aggtacatgg gtaacggtgg
     4561 cgactggcag cgcaaaccag gctttgtgac cacccataaa cctaaagtgg gctatgtcgt
     4621 ctcatttgca ccaggccaag caggagcaga tgcaacctat ggtcacgttg ctgttgtaga
     4681 gcaaatcaaa gaagatggtt ctatcttaat ttcagagtca aatgttatgg gactaggcac
     4741 catttcctat cggacgttca cagctgagca ggctcgtttg ttgacctatg tcgtagggga
     4801 caaactccca agaccataac cccacaacca agaagctacc tgcctgttga ccaatctcaa
     4861 aaagagacag tgtcaactta gaatgaggtt catcagatga taaagaaagt gaccaccccg
     4921 agtcaaaaaa taaaaaaacg tgttcgtaat ggctatctcc taaaactagg tacagcctgt
     4981 ttactcttaa gtatactcag ttatggcatt ggtcttttgg gccagcctag catggaaaat
     5041 acgtttatgg gaatagcgag tgttgccatg ttaggaagtg tctgtttctt tattatcttt
     5101 gctctaaacc gtatctttga cgcactagag gacaatttgc gagattagag taataggtca
     5161 aataatcttc caataattta gagcacaagc taagttattg gaagtttttt tgtcttttga
     5221 gagccatgat gccatcaaaa agtcaaacac tccttatgat gtgagagtca catgactaaa
     5281 attaaaagca aaatagaccc caaagccctg ttttctcaaa acagccaacg aaaaaagaca
     5341 agttaaccag ttcataaaaa acatcgtaga aacgttttca agagctaatg ttggagtaaa
     5401 ttgactgaag tatgatagaa tttttaatga atgtgacaat aatgtcacag ataaaaaccg
     5461 aaaacagcaa aaataattaa attgaatata aaaaagttaa ttaagtgagt gataatcaag
     5521 aaatgaagtt caaaaaatat aaataaaata aaacacaaga actgctaaaa gcagtgaatg
     5581 acataaatgt cgctaaaaaa atagatagaa gagtctaaaa tttcagtttt aggtcacgtt
     5641 ttatgaatgg aaatcagaat gatgacctta agttgtgatt tgcgcgtcat tgaccgcttt
     5701 cttcgtttta atgaaagaat ttgaaagata ttttatggta tacttttcct taatatggtt
     5761 catacggact tgataaatct atttacaaaa ataataaaaa attaagataa ggacatgaag
     5821 ttaatgcatg taagtaaatt gtttactagc caacaatgga gagaattgaa actgatctca
     5881 tatttaacag aaaactccaa tgctatcgga gttaaagata aggagcttag caaggctctc
     5941 aatatttcca tgttaacatt acagtcatgc ctcactaata tgcagtttat gaaagaagta
     6001 ggtggaatta cctacaaaga cggctatatt aacatttggt atcaccagtg ttgtgggcta
     6061 caagaggtct atcaaaaagc tcttcgagaa tcgccttctt tgaaattact ggagcttttg
     6121 tttttcagag actttagttc cttagaagag ttagctgaag agctgtttgt cagcttatct
     6181 accctcaaac gcctcatcaa aaaaaccaac acttacttgt ctcatacctt tgctattagt
     6241 atcgtgacaa gtccagtgca ggtgtcagga gatgagaggc aaattcgcct gttttacctc
     6301 aagtattttt cagaagccta taaaatctca gaatggccat ttggggacat tttaaacctg
     6361 aaaaactgtg aacgcctatt gagcttattg atcaaagaag tagatgtcaa agttcatttt
     6421 acgctatttc agcacttaaa aatcttaagt ggcgtcaact tgatccgtta ctacaagggg
     6481 tattcttgca gttataacaa taaaaaaacc agtcaccgtt tttctcaact tatccaacat
     6541 tcctcagaaa tccaggatct ttctcgcttg ttctacctca aatttgggtt acatctcgac
     6601 gagtatacca tagctgaaat gttttctaac catcttaacg acaagctaga aattggctgt
     6661 gcttttgaga ttatcaatca agacccaata tcaggaggca gacaagtaac caactggatc
     6721 catctattag atgagatgga aatcaagctc aatctcagca tcaccaataa atacgaagtt
     6781 gccgtaaccc tccataacgc ttcggtgtta aacgaagaag acattacagc caattatttg
     6841 ctgtttgatt ataaaaagag ttacctgaac ttttaccaaa aagaacaccc tcgtatctat
     6901 gaggcctttg tgacaagtgt cgagaagctg atgcaggcag ataatgctca agttagcaaa
     6961 gaattgatca atcagctcac ttattgtttc tttattacct gggaaaatag tttcttaaaa
     7021 gtaaatcaaa aagaaaaagt acgactccta gtgatagaga gaagttacaa tagtgtcggt
     7081 aattttttga aaaagtacat cggtgagttt ttcagcatta ctaattttga tgagttagat
     7141 tgtctgacaa ttgatctagt agagattgaa aaacagtacg atgttatcgt gacagatgtt
     7201 atggtgggta aaagcgaaga gttagagatt ttctttttct acaagatgat cccagaagct
     7261 attattgata gattaaacga atttttgaac gttagtttca cagataacaa tgttatggtc
     7321 aaaaacctcg aagccccttc tccttcaaaa tctcatagcg acaaagaggt acaaaagcca
     7381 gaaaagccag acaattctgt taaacaagca acatcagcaa catcatcata ggatttcaga
     7441 cgtcatggta agaaaaaaag aatcatttac agcccataga ggtaaggtca aaagctgaaa
     7501 acagctcaaa aaaactgacc tttacctttt ggcttttttt atttagaata attttattgg
     7561 agagatgctt aatctaattt aagcacaatt cttagaaagt taagaaataa ggagtaaaca
     7621 atgtctaaaa gaaatccaaa caaacactac tcactgagaa agttaaaaac aggtactgca
     7681 tcagtagcag tagctttgac agttttgggg actggattag caaacacaac tgatgtaaag
     7741 gctgagactg agcatgttga tgttgtgctt tcagcaaaag aagctaacaa agtattcgaa
     7801 gagcgcaaag ccttggaaaa acaagcgcgt gatttgggtg acactattaa tcacatgtca
     7861 caaaccatta gtgagcaaag ccgcaagatt gcagcattaa agtctgaagc agaacttaaa
     7921 aaccaacaag ctcttgaagc tttaaataat aaaaatgagc agattgctaa attaaccaac
     7981 gaaaacgcac agttaaaaga agccattgaa ggttatgtgc aaactatcca aaacgctagc
     8041 cgtgaaatcg cagcaaaaca acaagaactt gcagctgcaa aaagccagtt agaggcaaaa
     8101 aatgctgaga ttgaggcatt gaaacaacaa gatgcctcta agactgagga aattgctaaa
     8161 ttgcaatcag aagcagcaac tttagaaaac ctcctaggtt cagctaagcg tgaattgact
     8221 gatctgcaag ctaagctaga tacagctaac actgaaaaag aaaagctcca atcacaagca
     8281 gcaactttag aaaacctcct aggttcagct aagcgtgaat tgactgatct gcaagctaag
     8341 ctagatacag ctaacactga aaaagaaaag ctccaatcac aagcagcagc cctagaaaaa
     8401 caactagaag caactaaaaa agagttagct gatttacagg ctaaattagc agcaaccaac
     8461 caagaaaaag aaaagttaga agctgaagca aaagctctta aagagcaatt agcaaaacaa
     8521 gctgaagagc ttgctaagct aaaagcagat aaagcttcag gagctcaaaa accagatact
     8581 aaacctggca ataaagaggt tccaacaaga ccgtcacaaa caagaacaaa cactaataaa
     8641 gctcctatgg cgcaaacaaa gagacaatta ccgtcaacag gcgaagaaac aaccaaccca
     8701 ttcttcactg cagcagctgc aacagtgatc gcatctgcag gcgtacttgc cctaaaacgc
     8761 aaagaagaaa actaagccca atccacacta tcttttctag cccaagaaaa aaacaaaaaa
     8821 agaggaagac cattcctctt tttttgaacg gttaaacagc aaaaaggtca aaaaggtact
     8881 aaagtctcaa aaacctggtc tttacctttt accgcttatt ctttagaata gaattattag
     8941 agagaagtct tagaaaaatg aggctaattc cctaaagatg aaaaaataag gagcaaataa
     9001 tggctagaaa agatacgaat aaacagtatt cgcttagaaa attaaaaaca ggtacagcat
     9061 ccgtagcggt cgctgtggct gttttaggag caggctttgc aaaccaaaca gaagttaagg
     9121 ctgcggagtc tccaaaaagt actgagactt ctgctaatgg agctgataaa ttagctgatg
     9181 catacaacac attgcttact gaacatgaga aactcagaga tgagtattat acattaattg
     9241 atgctaaaga agaagaacct aggtataaag cattgagagg cgaaaatcaa gatcttcggg
     9301 aaaaagaagg aaaataccag gataaaataa aaaaattaga agaaaaagag aaaaatttag
     9361 aaaaaaaatc agaagatgta gaacgtcact atcttaaaaa actagatcaa gaacataaag
     9421 aacaagaaga acgtcaaaaa aatctagaag aactcgaacg tcaaagtcaa cgagaaatag
     9481 acaagcgtta tcaagaacaa ctccaaaaac aacaacaatt agaaacagaa aagcaaatct
     9541 cagaagctag tcgtaagagc ttaagccgtg accttgaagc gtctcgtgca gctaaaaaag
     9601 accttgaagc tgagcaccaa aaactcaaag aggaaaaaca aatctcagac gcaagccgtc
     9661 aaggcctaag ccgtgacctt gaagcgtctc gcgaagctaa gaaaaaagta gaagcagact
     9721 tagcagaagc aaatagcaaa cttcaagccc ttgaaaaact aaacaaagag cttgaagaag
     9781 gtaagaaatt atcagaaaaa gaaaaagctg agttacaagc aagactagaa gctgaagcaa
     9841 aagctcttaa agagcaattg gctaaacaag ctgaagaact tgctaaacta aaaggcaacc
     9901 aaacaccaaa cgctaaagta gccccacaag ctaaccgttc tagatcagca atgacgcaac
     9961 aaaagagaac gttaccgtca acaggcgaag cagctaaccc attctttaca gcagcagctg
    10021 caacagtaat ggtatctgca ggtatgcttg ctctaaaacg caaagaagaa aactaagcta
    10081 ttagactgat gctaaagtta agagagaatc aaatgattct ctctttttga gtggctaagt
    10141 aactaacaat ctcaattaga ccaaaaaatg ggaatggttc aaaaagctgg cctttactcc
    10201 ttttgattaa ccatatataa gtaaaaacat taggaaaata atagtaatat taagtttgtt
    10261 tcctcaataa aatcaaggag tagataatgg ctagacaaca aaccaagaaa aattattcac
    10321 tacggaaact aaaaaccggt acggcttgag tagccgttgc tttgaccgtt ttgggtgcag
    10381 gttttgcaaa ccaaacggaa gtaagagctg aaggggtaaa cccgactacg aacttgccag
    10441 agaaggctaa atatgccgca gtgaaagatg agaatactgg tttacgtggt gatcagaaaa
    10501 aattagtaaa aaaacttgaa gaagaacaag agaagagcaa aaatctagaa aagcaaaaac
    10561 aggagttaga aaaccaagcc cttaactttc aagacgtaat tgaaactcag gaaaaagaag
    10621 atctcaaaac aactttagct aagactacta aagaaaatga gatttcagaa gctagccgta
    10681 aagggttaag ccgagactta gaagcttctc gtgcagctaa aaaagagcta gaagctaagc
    10741 accaaaaatt agcagaagaa catcaagttt cagagactag ccgtaagggt ctaagccgtg
    10801 accttgaagc atcacgtgaa gctaataaga aggttacatc tgagttaaca caagcaaaag
    10861 ctcaactctc agcgcttgaa gaaagtaaga aattatcaga aaaagaaaaa gctgagttac
    10921 aagcaaaact agatgcacaa ggaaaagccc tcaaagaaca attagcaaaa caaactgaag
    10981 agcttgcaaa actaagagct gaaaaagcgg caggttcaaa aacacctgct accaaaccag
    11041 ctaataaaga aagatcaggt agagctgctc aaacagctac aagacctagc caaaataaag
    11101 gaatgagatc acaattaccg tcaacaggcg aagcagctaa cccattcttt acagcagcag
    11161 ctgcaacagt gatggtatct gcaggtatgc ttgctctaaa acgcaaagaa gaaaactaag
    11221 cctttagaac ttggtttttg taacggtgca atagacaaaa gtaagcaagg ccaaaaactg
    11281 agaaagtcct aaaaagctgg cctttacccc tcaaaattaa tgttttataa taaagatgtt
    11341 agtaatataa ttgataaatg agatacattt aatcattatg gcaaaagcaa gaaaaatagc
    11401 tgtatcatat gcaaataacc cctgtttgct ctttaaaaaa gacgttatcc ttatttctcc
    11461 acgcacagat ggacagctag gagagaatcg tttgattctc tcttttctta atggtcataa
    11521 agacaaagtc tctcatcatg aaaggacgac acattgcgta aaaaacaaaa attaccattt
    11581 gataaacttg ccattgcgct catgtctacg agcatcttgc tcaatgcaca atcagacatt
    11641 aaagcaaata ctgtgacaga agacactcct gctaccgaac aagccgtaga acccccacaa
    11701 ccaacagcgg tttctgagga agcaccatca tcatcaaagg aaactaaccc tccacaaatt
    11761 cctgatgacg cagaagaaac agtagcagat aaggctaatg atccagcccc tcaagctcct
    11821 gctaaaactg ctgatacacc agcaacctca aaagcgacta ttagggattt gaacgaccct
    11881 tctcaggtca aaaccctgca ggaaaaagca ggcaagggag ctgggactgt tgttgcagtg
    11941 attgatgctg gttttgataa aaatcatgaa gcgtggcgtt taacagacaa aaccaaagca
    12001 cgttaccaat caaaagaaga tcttgaaaaa gctaaaaaag agcacggtat tacctacggc
    12061 gagtgggtca atgataaggt tgcttattac cacgactata gcaaagatgg taaaaccgct
    12121 gtcgatcaag agcacggcac acacgtgtca gggatcttgt cagggaatgc tccatctgaa
    12181 acgaaagaac cttaccgcct agaaggtgcg atgcctgagg ctcaattgct tttgatgcgt
    12241 gtcgaaattg taaatggact agcagactat gctcgtaact acgctcaagc tatcagagat
    12301 gctgtcaact tgggagctaa ggtgattaat atgagctttg gtaatgctgc actagcttac
    12361 gccaaccttc cagacgaaac caaaaaagct tttgattatg ccaaatcaaa aggtgttagc
    12421 attgtgacct cagctggtaa tgatagtagt tttgggggca aaacccgtct acctctagca
    12481 gatcatcctg attatggggt ggttgggaca cctgcagcgg cagattcaac attgacagtt
    12541 gcttcttaca gcccagataa acagctcact gaaactgcta cggtcaaaac agacgatcat
    12601 caagataaag aaatgcctgt tctttcaaca aaccgttttg agccaaacaa ggcttacgac
    12661 tatgcttatg ctaatcgtgg tacgaaagag gatgatttta aggatgtcga aggtaagatt
    12721 gcccttattg aacgtggcga tattgatttc aaagataaga ttgcaaacgc taaaaaagct
    12781 ggtgctgtag gggtcttgat ctatgacaat caggacaagg gcttcccgat tgaattgcca
    12841 aatgttgatc agatgcctgc ggcctttatc agtcgaaaag acggtctctt attaaaagac
    12901 aatccccaaa aaaccatcac cttcaatgcg acacctaagg tattgccaac agcaagtggc
    12961 accaaactaa gccgcttctc aagctggggt ctgacagctg acggcaatat taagccagat
    13021 attgcagcac ccggccaaga tattttgtcg tcagtggcta acaacaagta tgccaaactt
    13081 tctggaacta gtatgtctgc gccattggta gcgggtatca tgggactatt gcaaaagcaa
    13141 tatgagacac agtatcctga tatgacacca tcagagcgtc ttgatttagc taaaaaagta
    13201 ttgatgagct cagcaactgc cttatatgat gaagatgaaa aagcttattt ttctcctcgc
    13261 caacaaggag caggagcagt cgatgctaaa aaagcttcag cagcaacgat gtatgtgaca
    13321 gataaggata atacctcaag caaggttcac ctgaacaatg tttctgataa atttgaagta
    13381 acagtaacag ttcacaacaa atctgataaa cctcaagagt tgtattacca agcaactgtt
    13441 caaacagata aagtagatgg aaaacacttt gccttggctc ctaaagcatt gtatgagaca
    13501 tcatggcaaa aaatcacaat tccagccaat agcagcaaac aagtcaccgt tccaatcgat
    13561 gctagtcgat ttagcaagga cttgcttgcc caaatgaaaa atggctattt cttagaaggt
    13621 tttgttcgtt tcaaacaaga tcctaaaaaa gaagagctta tgagcattcc atatattggt
    13681 ttccgaggtg attttggcaa tctgtcagcc ttagaaaaac caatctatga tagcaaagac
    13741 ggtagcagct actatcatga agcaaatagt gatgccaaag accaattaga tggtgatgga
    13801 ttacagtttt acgctctgaa aaacaacttt acagcactta ccacagagtc taacccatgg
    13861 acgattatta aagctgtcaa agaaggggtt gaaaacatag aggatatcga atcttcagag
    13921 atcacagaaa ccatttttgc aggtactttt gcaaaacaag acgatgatag ccactactat
    13981 atccaccgtc acgctaatgg caagccatat gctgcgatct ctccaaatgg ggacggtaac
    14041 agagattatg tccaattcca aggtactttc ttgcgtaatg ctaaaaacct tgtggctgaa
    14101 gtcttggaca aagaaggaaa tgttgtttgg acaagtgagg taaccgagca agttgttaaa
    14161 aactacaaca atgacttggc aagcacactt ggttcaaccc gttttgaaaa aacgcgttgg
    14221 gacggtaaag ataaagacgg caaagttgtt gctaacggaa cctacaccta tcgtgttcgc
    14281 tacactccga ttagctcagg tgcaaaagaa caacacactg attttgatgt gattgtagac
    14341 aatacgacac ctgaagtcgc aacatcggca acattctcaa cagaagatcg tcgtttgaca
    14401 cttgcatcta aaccaaaaac cagccaaccg atttaccgtg agcgtattgc ttacacttat
    14461 atggatgagg atctgccaac aacagagtat atttctccaa atgaagatgg tacctttact
    14521 cttcctgaag aggctgaaac aatggaaggc ggtactgttc cattgaaaat gtcagacttt
    14581 acttatgttg ttgaagatat ggctggtaac atcacttata caccagtgac taagctattg
    14641 gagggtcact ctaataagcc agaacaagat ggttcagatc aagcacc
//
LOCUS       DAUVUY010000048        14634 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.048, whole genome shotgun sequence.
ACCESSION   DAUVUY010000048 DAUVUY010000000
VERSION     DAUVUY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14634
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.048"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            259..1101
                     /locus_tag="VOX58_001197"
     CDS             259..1101
                     /locus_tag="VOX58_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HES5878427.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(1230..1952)
                     /locus_tag="VOX58_001198"
     CDS             complement(1230..1952)
                     /locus_tag="VOX58_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878428.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            2154..3785
                     /locus_tag="VOX58_001199"
     CDS             2154..3785
                     /locus_tag="VOX58_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HES5878429.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKLFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADGKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            3902..5050
                     /gene="nagA"
                     /locus_tag="VOX58_001200"
     CDS             3902..5050
                     /gene="nagA"
                     /locus_tag="VOX58_001200"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HES5878430.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLGLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKTPRLEEFDAWQKAAK
                     GLIKKIALAPEREGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            5172..6011
                     /locus_tag="VOX58_001201"
     CDS             5172..6011
                     /locus_tag="VOX58_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HES5878431.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDRLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTQEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            6143..6676
                     /locus_tag="VOX58_001202"
     CDS             6143..6676
                     /locus_tag="VOX58_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HES5878432.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            7048..7965
                     /gene="glyQ"
                     /locus_tag="VOX58_001203"
     CDS             7048..7965
                     /gene="glyQ"
                     /locus_tag="VOX58_001203"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HES5878433.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDDE"
     gene            8343..10382
                     /locus_tag="VOX58_001204"
     CDS             8343..10382
                     /locus_tag="VOX58_001204"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HES5878434.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQCVIVDAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGNFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYTNKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAVAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRVAILSDLVSKAKTIAAFNQLNTK"
     gene            10536..10793
                     /locus_tag="VOX58_001205"
     CDS             10536..10793
                     /locus_tag="VOX58_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HES5878435.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            10920..11312
                     /locus_tag="VOX58_001206"
     CDS             10920..11312
                     /locus_tag="VOX58_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HES5878436.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            11672..13198
                     /gene="glpK"
                     /locus_tag="VOX58_001207"
     CDS             11672..13198
                     /gene="glpK"
                     /locus_tag="VOX58_001207"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HES5878437.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQLA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            13214..>14634
                     /gene="glpO"
                     /locus_tag="VOX58_001208"
     CDS             13214..>14634
                     /gene="glpO"
                     /locus_tag="VOX58_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HES5878438.1"
                     /translation="MEFSRETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDETRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGE"
BASE COUNT         4405 a         2645 c         3263 g         4321 t
ORIGIN      
        1 tatcagtagc aaaaaccgac ttaataatga tataatttta tgggaaaagt cttagtttgt
       61 caactaaagt tagttatcag gagcctcatt acctccttgt atagctctat tggatgactt
      121 tactaactat tgaaaaggct agtaataaaa cataggattt aatgattagt tattaccatt
      181 agcctagaca tcttagcgaa ggcttaataa tctggtcttt gttaccgaac acaaattaga
      241 gaaaaagaag gataaaaaat gacttggaaa attgtaacag attcaggatg tgatcttaga
      301 agtttgacta gacaatccaa agagttgcgc tttgaacgtg tgcctttgac cctacagatt
      361 ggcacagaaa tttttagaga tgatgatgga ttagatatcg ataatatgat gacaactatg
      421 taccagtcgt ctaaagcaac gacatctagc tgtccaagcc cagaagcctt tttgcaggcc
      481 tatagaggtg cagataatgt tatcgtgatg acaattacag ggaccttatc tggcagtcat
      541 aatagtgcac gactggctaa aaatgaactt cttgaagaaa atcctaatgt caatattcac
      601 ctaatcgatt ctttatcagc aggtggcgag atggatttgc tcgtactgga gcttgagcgg
      661 ttgatcaatc ttggattatc ctttgaagaa gttgtcaaac agatcacagc ttatcagcaa
      721 aaaacacgtt tgatttttgt gcttgccaag gttgataatt tggttaaaaa tggtcgactg
      781 agtaaattag taggtaaagt aattggctta ctcaatatta gaatggttgg gaaagctagc
      841 aataaaggaa cgcttgaact tcttcaaaaa gcgcgtggtc aaaaaaaagc agtttctgct
      901 ttgatagaag aaattcaaaa agagggttat gtaggcggaa aagtttacat tgctcatgct
      961 caaaatccaa aaatttgtga gcagatcagt gaaaaaatca aatctttata tcctgatgct
     1021 gtgattcaaa ctggaaggac ctcaggtttg tgtagctttt atgctgaaga cggaggactt
     1081 ttaatgggct acgaaattta atgctatcta acaaaacaaa aagctagact cttttgattt
     1141 gagctagctt ttttaagata tcaggaagac taatcactaa gatagccgtc taaaacgttt
     1201 attgattgat tttacgaaaa atttttgtat tactcttttg atgattgctt gtctcctgag
     1261 tcagaaggtt ctgaagaggt ctgaggtgga attggatcag gatgactagg ttgtggcgta
     1321 acatctgaag ctgaagattc tgagcttggg tctaaagtag gtgactggga attatcataa
     1381 gtatctgaga cgtctcctgg gaaaggtgtc cagtaagagt tatctgttat ttctccactt
     1441 tgtcttggaa attcatcaat aatgggtttt gctggtaaaa tagcgtctaa ccgagattgt
     1501 aaagcggctt ttttaccttt gttacttaag tcgttaactg ctttttgggc gatatcttta
     1561 agattttgat tgagagtctc ctcagcagct ttaactgctt gagtagctgc atcttcttgc
     1621 tcttgctttg ctttggcaac gtttagctgt tcttgtagcg tttttttctt agaagattct
     1681 gctaatttat caacagcttt ctgggcttct tggatagcag ctgcagtggc taaacgatca
     1741 gcgtcattaa ctgctttaac tgcttttttt agtaaactag cagcactttt agaatgttta
     1801 gctaccttat cttgcgtttg agggtgggat ccaaaaataa ggcaggagcc acctattaga
     1861 gtgatagtta gaacactcaa catccaataa agcgcctctt tttgtgattt agtcatggaa
     1921 tctcctttat gatattgttt catcgtagcc atattatacc tctaattaaa cgaaatgaaa
     1981 agatttgtgc aaaaacaaat gatgtaaaat atcggaaaag cctaacacct tatcaaacca
     2041 ataatatgca aatgttgaca tcggtataca cagaaacgct taaaaaaacc ttgtgataaa
     2101 gaccgtaata cattaaaata gaatagacta aaaaattgag cgaggacaat attgtgtcag
     2161 taaattggca agacattgca tttcatttct ttggaggctt aggtctcttt cttttcagta
     2221 tcaagtatat gggagatggt ctgcaacaag cagcaggaga taaactaagg tattacattg
     2281 ataaatacac cagtaatccc ttttttggta tcttagtcgg aattgccatg tctgccttaa
     2341 ttcagtccag ttcaggcgta actgtgatta cggttggttt ggtttctgcg ggtttgctaa
     2401 atttgcgaca agccattgga attgttatgg gggctaatat tggaacgact atcacttcgt
     2461 ttttaattgg ttttaaacta ggagattacg ccttgcccat gatttttatt ggtgcagcct
     2521 gtcttttctt tacctctaat aaaaagctca acaattttgg gcgtatcatt tttggtgtcg
     2581 gaggaatttt cttctcgctg aacctaatgg gagatgccat ggatcctttg aaatctgtgt
     2641 cagcctttca aaattattta gcgacattag gtgataaact cttccaaggt gtctttatcg
     2701 gaacggcttt gaccatgttg attcagtcat cggcagccat tatcgggatt ttacagggtc
     2761 tgttttcagg tggtcttttg actttacagg gagctattcc tattttgtta ggctcaaata
     2821 taggaacctg cattacagct gtcttagcag ctataggttc aaatattgct gctaaacgtg
     2881 tggcagcagc tcatgtgtta tttaatttga tcggtacaat tatttttatg attatcctag
     2941 tcccattcac atcattgatg ttatggctac aatcaaagtt gagtttaacg cctgagatga
     3001 ctatcgcttt ttctcacggt tcttttaata tcactaatac catcttatta attcctttca
     3061 ttagtctact agctatgatt gtgactagac tcattccagg agaagatgaa gtggtcaaat
     3121 acgaagccct ttatttagat cgcttactta ttacccaagc cccttctatc gcactaggaa
     3181 atgcccacaa ggaattggtt catttagcct cttatgcgat tcaagctttt gaagcttctt
     3241 acagttatat tatgacagct gacggtaaat ttggggaaaa ggtcaagcgt tatgaaagag
     3301 cagttgatac catcgatgag gagttaacca cttatttggt tgatatttct aacgaagccc
     3361 tcagcccaag tgaaaatgaa gtcttggctg gtattcttga ctcttcacgt gatttggaaa
     3421 gaattggaga tcattctgag tctttaggca ttctaattga aggcatcata tctaaacaaa
     3481 ttggtttttc aatatctgct cgtcaagaat tgacagagat gtatcaattg acgcattgtt
     3541 taacactgga tgccattaga gctatcgttg atagtgatac agacttggcg caaactattg
     3601 tgactcgcca taaagaaatt gaagaaaaag agcgacgatt acgtaaaacc cacattaagc
     3661 gcttgaattg tggagaatgt acggcacaag caggtattaa ttttattgat attatttccc
     3721 actatacccg catcacagat catgccttga atttagcaga gaaagtactt tcacaccagc
     3781 tttaaaaaag agaaaacgtg aatgatcatc cacgttttct tttttgaatc caaaaagtgg
     3841 tatataccat ttacaaagta aagaataagg cgtataataa ggaaaaaagg gaggaaaaaa
     3901 gatgacatgc tatcttaaag cagattgttt ttattaccca acagaggtga aaccggctgg
     3961 ctacctaggt cttcatgatg gtgtttttgg ggagtggaca gaaattgttc cggcagatgc
     4021 gcaaatcatt gactacaccg gttatcaaat tgcaccaggt cttgtggata cgcatatcca
     4081 tggctatgcg ggagctgatg tcatggataa ttctgcccaa gggattcatc aaatgagtga
     4141 aggactatta gcaacaggag taacgtcttt tttaccgacg accttaactt ctacctttga
     4201 gcagttagaa aaagtatcag ggactattgc ttcagttgct gatcaggtaa aaggtgctaa
     4261 aatacaaggt atctactttg aaggtcctta ttttaccgaa gaatataagg gtgctcaaaa
     4321 cccaagttat atgaaaaccc cacgcttgga ggaatttgat gcttggcaaa aagctgctaa
     4381 agggctcatc aaaaaaattg cccttgctcc tgagcgtgag ggtgtcaaag aatttgtgtc
     4441 tgcagtgaca aaacaaggcg taacagtggc cttagggcat tcaaatggta cttatcaaga
     4501 ggctaaagaa gctgttcaag ctggtgctag tgtttgggtt catgcttata atggcatgcg
     4561 aggattgacc caccgtgaac caggaatggt gggagcggtt tataatcttc caaatactta
     4621 cgcagagttg atttgtgatg gccaccatgt ctcaccaatt gcttgtgata ttttgatgca
     4681 gcaaaaaggg catgatcatg tagccatgat tacggactgt atgcgagcag gtggttcacc
     4741 agatggtgat tacctcttgg gggaattctc tgtggttgtg gcaaatggca ctgctcgtct
     4801 taaagagagt ggaaacttgg caggatctat tttgaaatta aaagacggcc ttagaaatgt
     4861 agtggcctgg ggaatcgcaa ctccggcaga agccattcat atggcaactt atgtgccagc
     4921 tgtttctgtc ggtattgacg acgtatgtgg tcaaatcaaa gctgggcatg cggctgactt
     4981 tattgttctt gataaggatt taacacttgt tgcaacctat ttaaatgggc aaaaagcttt
     5041 tgatgcctaa gagatacaaa aggattggag tagctatgtc tggcaggcca attctttttg
     5101 tattttcttt ctttatgata gaatagatta aagtaatcgc ttacagatat tgataaaagg
     5161 aggtttttgt gatggtaaca acagtaaaaa tgacaagtgg ctatgagatt cccgttttag
     5221 gatttggaac ttaccaagca gccgatggtg aggaagctta ccaatctacc ttagcagcca
     5281 tcaaggctgg ctatcgtcat attgatactg cagctattta taaaaatgaa gaaagtgtgg
     5341 gtcgtgccat caaggattct ggggttctac gagaagactt atttattaca actaaactct
     5401 ggaatgacgc ccatagttat gaaggggcaa aagacgcttt agctgcttct cttgaccgtc
     5461 ttggtctgga ttatgtggat ctctatctta tccattggcc aaacccaaaa gctctgcgta
     5521 acacttggaa agaggcaaat gcccaagcat ggcaatatat ggaagaggcg gttgaagcag
     5581 ggcttattaa aagcattggc gtcagcaatt ttatggtgca tcatctagaa gctcttcaag
     5641 agacagctaa aataactcca gcaattaatc agattcgatt agccccaggt tgctatcaaa
     5701 aggaagtggt tgattattgt aaggcaaatg agattttatt ggaagcttgg agtcctctag
     5761 gacaaggaga gatttttgat aatgagacta tgcagcagtt agctaataaa tatgacaaaa
     5821 cagtggcaca agttgcctta gcttggtcgc tggcagaagg ttttattccc ttgccaaaat
     5881 cggtgcatga tgagcgcatc aaggaaaata tggctatttt tgatgtgagc ttgacacaag
     5941 aagataaaaa aacaattcgc tatctctcag gaatgtcagc gattcctaac cctgatacta
     6001 caagttttta aggaactaca aagatttagc cataattcgc taaatctttt ttgaaaacgg
     6061 tttgacaaag gtttatcatt tagctataat ctttattagt gttttgtgaa agaaactatt
     6121 attgaaaata aggagccgta ttgtgaccat ctttttagtg atgttagcta tgtttactat
     6181 tcgtttagta tttttgaaga aatcgataac aaatgaaaaa gccatattag cccaaggagg
     6241 acaggaattt ggtgctcaga acacgaaatt tttgacgtta cttcacatta tgatttatgt
     6301 gtttgcggtg attgaagcgc tgcttaaaca aattaagttt gatggcatta gttttttagg
     6361 tcttttgttg atgttattat cagtagctgt tttatatgaa gtgacacgta tcttaggtga
     6421 catttggact gtaaaattga tgttagcaaa agaccacaaa tatgtggatc actggttgtt
     6481 caaaacgatt aaacatccta actacttttt aaatatcgcc cctgagctgg tgggcatagc
     6541 cttactttgc catgctaaaa ttacggctat gcttcttttc ccatgctaca ttgtagtaat
     6601 atatttacgt attcgagaag aaaacaagct attagcggaa gtgattattc caaacggtac
     6661 tcgaacaaaa ccttaagctc atgatggaca aactaatggc tttatgctaa actagactct
     6721 aggaaaaagg atgcaagtat cttatctaaa gcgagttcgg ggtagttaga gccgaatggt
     6781 aggactgcag attggcgctt ccgtttgggc agtgtgatta agtatatttg tcaatattgc
     6841 ccaaaaagat actatataaa tgaagtaata aattagggtg gaaccgcgtt ttgacgcccc
     6901 taggtcttat gatttaggag catcaaaatg cggttttttg gtgcctagac ctgtaataca
     6961 tgctagtaac atcgtcaggt attttactga tgacaaaaaa acgattgtca atgatatacc
     7021 attttactac tatcaaagga gtacgaaatg tctaaaaaac ttacctttca agaaatcatc
     7081 ttgaccttac aacaatattg gaatgatcag ggttgtatgc tcatgcaagc ctatgacaat
     7141 gaaaaaggag ctgggaccat gagtccttac acgttcttac gtgctatagg tcctgagcca
     7201 tggaatgcgg cttatgtaga gccatcacgt cgtcctgcag atggccgtta cggagaaaac
     7261 ccaaatcgtc tttaccaaca ccaccaattt caagtggtca tgaaaccatc accatcaaat
     7321 attcaagaac tttaccttgc ttctttggaa aaattgggca tcaatccttt agaacatgat
     7381 attcgttttg ttgaagataa ctgggaaaac ccatcaacag gatcagcagg gcttggttgg
     7441 gaagtttggc ttgatggaat ggaaatcaca caatttacgt atttccaaca agttggtggc
     7501 cttgcgacat ctccagtgac agcagaagta acttatggtc ttgagcgctt agcttcctat
     7561 attcaagaag tggattcggt ctatgatatt gagtgggcac caggtgttaa atatggtgaa
     7621 atcttcttac aacctgagta tgagcattca aaatattcct tcgaaatatc agaccaagac
     7681 atgctgcttg aaaactttga aaagtttgaa aaagaagcct cgcgtgccct agaagaaggt
     7741 ttggttcacc cagcttatga ttacgtgctc aaatgttctc atacgtttaa cctgctggat
     7801 gcccgtggcg ctgtttccgt cacagaacgc gcaggctaca ttgctcgtat ccgtaatttg
     7861 gcacgtgtcg ttgctaagac ctttgttgca gaacgcaaga agcttggatt tccactgttg
     7921 gatgaagcaa cacgcgcaat cttgctagct gaagatgacg aataagtcga tttaaagatg
     7981 atctagttgc gagctttgta atcttaagag cgagcaagaa gtgggcgacc gaaggtcgcc
     8041 gagtcaaggt tactcccgct gtagtgacaa ttcggtaagg ttacttatcg aacgagggag
     8101 ttggcgagac tttaggctgg ctaactagga atggaacact tttgttttct gaaattatcc
     8161 actggataat ttcactttgc gtccccccta cctaagggaa caatcaaaaa gatacttttt
     8221 agagaaagga actgaacatg ggctacggac tatgtcaaaa agaccaagac ttcctaggtg
     8281 caagcacctg cgtcgctttg ctattttgat ggtgtccgct taacgccctt ggtatcttaa
     8341 ttatgagtaa aaatctacta atcgaacttg gtctggaaga acttccagcc tacgtggtca
     8401 caccaagtga aaaacaactg ggagagcgct tggcgacttt cttaacagaa aatcgtctct
     8461 cttttgaaga tatacaaacc ttttcaactc cacgccgttt ggcagtgcgt gtgtctggat
     8521 tagctgatca gcaaacggat ttgactgagg actttaaagg gcctgctaag aaaattgccc
     8581 ttgatgctga tgggaatttc tcaaaagctg ctcaaggatt tgttcgtggc aaaggcttga
     8641 ccacagatgc tatcgaattc cgcgaagtca aaggagaaga atacgtttat gtgaccaaac
     8701 acgaagctgg aaaacctgct aaggaagtgc ttctaggcgt tactgaagtg ttgtcagcaa
     8761 tgaccttccc agtcagcatg cactgggcta acaatagttt tgaatatatt cgtcctgttc
     8821 acaccttgac agttctgttg aatgatgagg ccttggaact tgacttcttg gatattcatt
     8881 ctggacgcgt cagccgtggt caccgttttt taggtactga aaccactatc acaagtgctg
     8941 attcttatga ggctgatttg cgcagccaat gcgtcattgt tgacgctaaa gagcgtcaag
     9001 agatgattgt ggagcaaatc aagacgcttg aagtggagca gggtgtgcaa gttgacattg
     9061 acgaagactt gcttaatgaa gttttgaact tggtcgagtt tccaacagct ttcatgggaa
     9121 attttgaggc taaatacctt gatgtcccag aagaagtatt ggtaacatca atgaaaaacc
     9181 accaacgtta ttttgtggta cgtgatcagg ctggtcactt gatgccaaac tttgtatcag
     9241 ttcgtaatgg gaatgatcaa gccattgaaa atgttattaa agggaacgaa aaagttttgg
     9301 tagctcgctt agaagatggt gaattcttct ggcgtgagga tcagaaattg caaattgctg
     9361 atttagttgc caaattgacc aatgtgacct tccatgaaaa aatcggctct cttgctgaac
     9421 acatggatcg tacgcgtgtc attgccgcat cacttgctaa ggaagccaac ctatctgctg
     9481 aggaagtcac agctgttgac cgtgctgccc aaatttacaa atttgacctt ttaacaggta
     9541 tggttggtga atttgatgag ctgcaaggga ttatgggtga aaaatatgct cttctagctg
     9601 gtgaagatgc tgctgtggca acagcaatcc gtgaacacta cttacctgat gctgcaggag
     9661 gtgcgctacc tgaaaccaaa gttggcgctg tcctggcctt ggcagataaa ttggatactc
     9721 tcttgtcatt cttttctgtt ggattgattc catctggctc taatgatcct tatgctctac
     9781 gtcgtgcaac gcagggtatc gttcgtattc ttgatcattt tggatggcgc atcccaatgg
     9841 ataagctggt agatagctta tatgacttgt cctttgatag tttgacctat accaataagg
     9901 cggatgtgat gaactttatc cgtgctcgtg tggataagat gatgggcaaa gccgcaccaa
     9961 aagacattcg ggaagctatt ctagcaagtt caacctttgt ggttccagaa atgttagcgg
    10021 tagctgaagc tcttgtcaaa gccagccata cggaaaatta caaaccagca gttgaatctt
    10081 tgtcacgtgc ttttaatttg gcagaaaaag cggacgcttc tgttcaggtg gaccctagcc
    10141 tctttgaaaa tgagcaagaa aacactttgt ttgcagctat tcaaggctta actctagcag
    10201 gcagtgctgc ccagcagtta gaacaggtct ttgcccttag cccagtgatt aatgatttct
    10261 ttgataatac aatggttatg gcagaagatc aagcccttaa gaacaaccgt gtagctattt
    10321 tatcagattt agtaagcaaa gctaagacca ttgctgcttt taaccaacta aacaccaagt
    10381 aaatagaaag gaaagagccg ttggggactt agctagaaaa gtgagttcag tacccgagtg
    10441 gaaagaacaa ggcctttaaa cattgctttt cacagtgaga gtcaagtcag ttttttaagt
    10501 cttacacttt tttcccaggc tcttagtggc ttaacatgga tcctaaaaaa attgctcgta
    10561 tcaatgagct ggctaaaaag aaaaaaacgg taggcttgac tggacctgaa aaagtggaac
    10621 aggcaaaact ccgtgaagaa tacattgaag gctatcgtcg ctctgtcaga caccatatcg
    10681 aaggcatcaa attggtggac gaagaaggaa acgacgtgac ccctgaaaaa ttaagacagg
    10741 tgcaacgtga aaaaggcttg cacggccgtt cattagatga cccaaaatca taagacaaaa
    10801 agcagctctg aactgctttt tgtttatcgt gatgactgtt agcttttatt ttaaatggta
    10861 tgatacttca aagtgaccac acgtttttat attgtatagt caaaatattg ggaaaaacca
    10921 tgtgtttgag ttataataga agaaatcaaa gaaagaaggt agtacttatg acaattaaaa
    10981 aattctctct actagccata gccagcctct ctttactcag tcttgcagcc tgtgatatgg
    11041 atgataaaga cgatcatatg gataatcaac caaagacaag tcaaactagc aagaaagtta
    11101 aactttcaga agacaaagcc aaatcaattg cgttaaaaga tgcttctgtt acggaagctg
    11161 atgctcaaat gctatcagtt acacaagata atgaagatgg caaagccgtg tatgaaatcg
    11221 agtttcaaaa caaagatcaa gaatacagct ataccattga cgccaactca ggggatattg
    11281 tggaaaaatc ttctgaacca atcaatgact aatcaagaac tgtaatgaat aagacagcct
    11341 taactcttgt gtccaattgt ctttgcctct taggcgcggc tgtttgatac cgtgttgcct
    11401 ttgaggctta gttctcttta tgacccaatg atgactctgg attagcagag tgattgttgg
    11461 gtcttttgat tttctttgat aagaaagatt acctcgttag aaatgaggag acgtggttgt
    11521 catcagtgga tttttcaaga tcatctagtg ggataagcta cctatcttat cagctttttt
    11581 ttagctttaa aaattcttga taatatattt ctgataaaat tagctataat agaatcaaat
    11641 aggaaaacgc ttacttaaag gagggtaatg aatgtcgcaa gaaaagtata tcatggctat
    11701 tgatcaggga acaacaagtt cacgagcgat tattttcaat caaaaagggg aaaaggttag
    11761 cagtagccaa aaagaattcc ctcagatttt cccacacgct ggttgggtag agcacaatgc
    11821 taatcagatt tggaattcgg ttcagtctgt tattgcgggt gcttttattg agtccagcat
    11881 caaaccaagc caaattgaag caatcggaat taccaaccaa cgtgagacca cagtggtttg
    11941 ggacaaaaaa acgggtgtac ctatttataa tgctattgtg tggcaatcac gacagacggc
    12001 accaattgct gagcaactca aacaagatgg ccatacaaaa atgatccatg aaaagacagg
    12061 ccttgttatt gatgcctact tttctgcaac caagatccgt tggattttag atcatgttcc
    12121 tggtgctcaa gaacgagctg aaaaaggcga gttacttttt ggaaccattg atacttggct
    12181 ggtttggaaa ttgacagatg gtgctgttca tgtgacggat tattccaatg cggctcgtac
    12241 catgctctat aatattaaag acttaacatg ggatgatgag attttagagc ttttaaatat
    12301 tccaaaagat atgcttccag aggttaaatc caattctgaa atttatggca aaacagctgc
    12361 ctttcatttt tatgggggcg aagtgcctat ttcaggtatg gcgggtgacc aacaggcagc
    12421 tttgtttggc caactagctt ttgagcctgg tatggtgaaa aatacttatg gaaccggttc
    12481 tttcatcatc atgaatactg gtgatgagat gcagctatcg agtaataacc ttttgaccac
    12541 cattggttac gggattaatg ggaaggttca ctatgcttta gaaggctcta tttttattgc
    12601 aggctctgcc attcaatggt tgcgagatgg tcttaaaatg attgagacct caccagagtc
    12661 tgaacagctg gcactagcat ctaccagtga tgatgaagtc tacgttgtgc ctgcctttac
    12721 cggtttagga gctccttact gggattctaa tgcgcgtgga tctgtctttg gcttgacacg
    12781 tggtaccagc aaagaagact ttgtcaaagc aaccttacaa tctatcgcct atcaagtgcg
    12841 agatgttatt gataccatgc aggtggattc aggtattgac attcaacagc tccgtgtgga
    12901 tggcggtgca gccatgaata atatgctcat gcaatttcag gctgatattt taggaattga
    12961 tattgcccgt gctaaaaatt tagaaacaac agctctagga gcagctttct tagcaggact
    13021 tgctgtgggc tactgggaag acatggatgc tttgaaagag ttaaatgcca caggccagct
    13081 ctttaaggct agcatgaatg aatcgcgcaa agaaaaatta tacaagggtt ggaagcgcgc
    13141 tgttaaagca actcaagttt tcacccaaga agaagatgca gatgacgatg caaaataact
    13201 agaggaggat ggaatggaat tttcaagaga aacaagacgc ttagctctcc aaaaaatgca
    13261 agaaagagat ttagacctac tgattattgg gggaggtatt acgggtgctg gtgtggcact
    13321 tcaggcggca gctagtggcc tagatacggg tctgattgag atgcaagatt ttgcgcaagg
    13381 aacctctagc cgttcaacca aattggttca cggggggctt cgttacttga aacaatttga
    13441 tgtggaggtg gtttcagata cggtgtcaga gcgggctgtg gtgcaacaaa ttgcacccca
    13501 cattccaaaa ccagacccta tgttattacc tgtttatgac gaacctggta gtacctttag
    13561 catgttccgt ttgaaggtcg ctatggattt gtatgatctt ttagcaggcg tgtccaatac
    13621 gccagcggcc aacaaggtgt taaccaaaga agaagtctta aaacgagaac cagacttaaa
    13681 acaagaaggc ttgcttggtg gtggggttta ccttgatttc cgtaataatg acgcaaggct
    13741 tgttattgaa aatatcaaac gagctaatcg tgatggggct cttattgcta gtcatgtgaa
    13801 agcagaagat ttcttgctag atgacaatgg taagattatt ggtgtgaagg cgcgtgattt
    13861 gctgtcagat caagaaatca ttatcaaggc taaattagtc atcaacacca caggtccatg
    13921 gagtgatgag actcgtcaat tctctcataa gggacaaccg attcatcaaa tgcgccctac
    13981 aaaaggggtg catctggtag tggaccgtca aaaattacca gtgtctcaac ctgtctatgt
    14041 tgacacaggg ttaaatgatg gtcgtatggt ctttgtcttg ccacgtgaag aaaaaactta
    14101 ttttggaaca acggacacgg actacactgg agacttggag cacccacaag ttactcaaga
    14161 agatgtggat tatttgttag gcgttgtcaa taaccgcttt ccaaatgcca acgtgaccat
    14221 tgatgatatt gaaagcagtt gggctggtct tcgccccttg ttatcaggta atagtgcttc
    14281 tgactacaat ggtggcaaca gcggtaaagt cagtgatgat agttttgatc acttggttga
    14341 tactgtcaaa gcctatatta accacgaaga tagccgagaa gctgttgaaa aagctattaa
    14401 gcaggttgaa accagcacat ctgaaaaaga attggatccg tctgcagtgt cacgaggttc
    14461 aagttttgac cgtgatgaga atggactctt taccttggca ggtggtaaga ttaccgacta
    14521 tcgcaaaatg gctgaaggag cattgacagg gattattcaa atcctcaaag aagagtttgg
    14581 caaatccttc aagcttatca attcaaaaac ctatcctgtt tcaggaggtg aaat
//
LOCUS       DAUVUY010000049        14102 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.049, whole genome shotgun sequence.
ACCESSION   DAUVUY010000049 DAUVUY010000000
VERSION     DAUVUY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.049"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..663
                     /locus_tag="VOX58_001209"
     CDS             <1..663
                     /locus_tag="VOX58_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HES5878439.1"
                     /translation="NVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDYRPYHLLPLL
                     PFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPILIVGTILIC
                     STYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKSVSQFSSPDI
                     NTQKASHRKILEDELLENKAAYIVVNRYKNLPKIIQKVLSTNYKVDKQITTKSFIVYQ
                     KK"
     gene            760..2958
                     /gene="nrdD"
                     /locus_tag="VOX58_001210"
     CDS             760..2958
                     /gene="nrdD"
                     /locus_tag="VOX58_001210"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HES5878440.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIIKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            3140..3286
                     /locus_tag="VOX58_001211"
     CDS             3140..3286
                     /locus_tag="VOX58_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878441.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            3315..4250
                     /locus_tag="VOX58_001212"
     CDS             3315..4250
                     /locus_tag="VOX58_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HES5878442.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKASLDGVFIHAATSAHAELASLFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKKLSSLSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLSEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            4259..4768
                     /locus_tag="VOX58_001213"
     CDS             4259..4768
                     /locus_tag="VOX58_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HES5878443.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLSSQGVPAIQYVAFDERSQAIGFLNLRLRLNERLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSYAISKNITTILVTCDETNVASRAVIVANGGVLEDSRGGTERY
                     WIEEQRGDK"
     gene            4768..5382
                     /gene="nrdG"
                     /locus_tag="VOX58_001214"
     CDS             4768..5382
                     /gene="nrdG"
                     /locus_tag="VOX58_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HES5878444.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRRELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(5556..6287)
                     /gene="yaaA"
                     /locus_tag="VOX58_001215"
     CDS             complement(5556..6287)
                     /gene="yaaA"
                     /locus_tag="VOX58_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HES5878445.1"
                     /translation="MLTFLIPTAKEMMIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQNLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(6329..7186)
                     /locus_tag="VOX58_001216"
     CDS             complement(6329..7186)
                     /locus_tag="VOX58_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HES5878446.1"
                     /translation="MTYPYNSTISLGTVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTSFLVPDRHSLGLVLNHFLTRGISLEGSAD
                     HGYSEAIYLSGPEGNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKR
                     FLLAQDTRIGHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKKARLHGLAILQEDSSSFTTEDEEGI
                     CVNVILGQD"
     gene            7373..7710
                     /locus_tag="VOX58_001217"
                     /pseudo
     CDS             7373..7710
                     /locus_tag="VOX58_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(7989..8702)
                     /gene="treR"
                     /locus_tag="VOX58_001218"
     CDS             complement(7989..8702)
                     /gene="treR"
                     /locus_tag="VOX58_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HES5878447.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            8913..10937
                     /gene="treP"
                     /locus_tag="VOX58_001219"
     CDS             8913..10937
                     /gene="treP"
                     /locus_tag="VOX58_001219"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HES5878448.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QQVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGTVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANSIG
                     VGGLPGFMAINVKYMIPFFICMVVAIVVPMFLAFFFRKSHIMTKTEDEAKLPETPVSD
                     APVATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGSLPRQIKANDKLAVAMKK"
     gene            11005..12633
                     /gene="treC"
                     /locus_tag="VOX58_001220"
     CDS             11005..12633
                     /gene="treC"
                     /locus_tag="VOX58_001220"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HES5878449.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAE
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYAGEVELELPPR
                     YQHGQALISNYEKVSICEKVMLKPYQTLAILADN"
     gene            12841..>14102
                     /gene="pepO"
                     /locus_tag="VOX58_001221"
     CDS             12841..>14102
                     /gene="pepO"
                     /locus_tag="VOX58_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HES5878450.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGDHIPDDAILKNFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDESLSLVENAQNLAKITIAHTWSK"
BASE COUNT         4175 a         2382 c         3183 g         4362 t
ORIGIN      
        1 aatgtcatta ggcgatttaa aaacaattct gatagggtcg tcaagtggct atttgtcatg
       61 gtcattctag aatctatact tgtggctata ttttcacaag actatcgccc ctatcatctt
      121 ttacctcttt taccttttgg attaattttg actgctattc ctgttggcta tcagtatggt
      181 ataggattag gtcagagtag tcatcgcaga cgtcatggta aaaatggtgt tggtcgagta
      241 atgatgattt atcttaagag acacttttat ttgccaattt taattgtagg gacaatacta
      301 atctgttcta cttattgttt cattagtagt attcctctta atcaggagcg tgatcatatt
      361 gctagttatt tagaacagaa actaaataaa actcaatcta tttatgtttg ggatgatact
      421 tctaaaattt atttggacag taaagctaaa tctgtttctc aatttagttc tcctgacatc
      481 aatacgcaaa aagcgagtca tcgaaaaata ttagaagatg aactattaga aaataaggct
      541 gcttatatcg ttgttaatcg ctataaaaac ctgcctaaaa tcattcaaaa agtattatct
      601 actaattaca aagtagataa acagataacg acaaaaagtt ttattgttta tcagaaaaag
      661 taatctaata tcaatatatt gtgttcgagc aaaaaacttg acacaatata ttgatttttt
      721 tagttgtcgt gatataatag ttccgtaagg agatttgata tggtaagttt agaagaagac
      781 aaggtgactg ttcaacctga tattaaagtg attaaacgag atggtcgcct tgttaatttt
      841 gatagtacaa aaatctatag tgctttatta aaagcaagca tgaaagtaac tcggatgtcg
      901 ccacttgttg aggctaaatt agaggctatt tctgatcgca ttatagcaga aattattgag
      961 cgttttccaa ctaatatcaa aatttatgaa atccaaaata ttgtagagca taagcttctt
     1021 gcagctaatg aatatgctat tgcaaaagaa tacattaatt atcgtactca gcgtgacttt
     1081 gcacgttcac aagcaacaga tatcaatttt tctattgata aattaattaa taaagatcaa
     1141 acagttgtta atgaaaatgc taacaaagat agcgatgttt ttaatactca acgagattta
     1201 actgctggaa tcgtagggaa atcgattggt ttaaaaatgt taccttcgca tgttgctaat
     1261 gctcatcaaa aaggagatat ccattaccat gatttggatt acagtcctta tacaccgatg
     1321 acgaactgct gtttaattga ctttaagggc atgttagcca atggctttaa aattggtaat
     1381 gctgaagtgg aaagtcccaa gtctattcaa actgcaacag ctcagatctc acagattatt
     1441 gcgaatgtag catcaagtca gtacggcgga tgcacagctg atcgcattga cgagttttta
     1501 gccccatatg cggagcttaa ctttaaaaaa catatggctg atgctaagaa atggatcgtt
     1561 gagactaaga gagaaagcta tgcttttgaa aagactcaaa aagatattta tgatgcgatg
     1621 cagtctttgg agtatgaaat taatacgctc tttacgtcta atggtcaaac accatttact
     1681 tctttaggat ttggtttggg gacgtcttgg tttgaacgtg agattcaaaa agctattttg
     1741 accattcgga ttaatggtct tggtagtgaa catcgcacgg ctattttccc taaattaatt
     1801 ttcacggtta aacgtggctt gaatttagaa ccagattcac caaactatga tattaagact
     1861 ttggctttag aatgtgcgac taagcggatg tacccggata tgttatctta tgataaaatt
     1921 attgatttga caggatcttt caaatctcca atgggatgcc gctctttcct tcaaggctgg
     1981 aaagatgaaa atgggcaaga tgtgacctca ggccgtatga atcttggggt tgtcaccctc
     2041 aatttacctc gcattgccat ggaatcaaat ggcgatatgg ataagttttg ggagctgttt
     2101 aatgagagga tgctaattag taaggatgct ttaatttatc gtgtcgaacg tgtcacagaa
     2161 gcaaaaccag caaatgcccc tattctttat caatatggtg cttttggaaa gcgtttggag
     2221 aagacaggga atgtaaatga tctctttaag aatcgtcgtg caacagtctc tcttggctat
     2281 attggtcttt atgaagtggc gtctgttttt tatggtggtc aatgggaagg taatccagat
     2341 gctaaagctt ttaccttgtc aattatcaag gcaatgaaac aggcctgtga ggattggtca
     2401 gatgaatatg gttatcattt ctctgtttat tcgactccat cagaaagttt gacagatcgc
     2461 ttttgtcgtt tagatacgga aaaatttggc attgtgacag atattacgga taaagaatac
     2521 tatacaaatt cttttcacta tgatgtgcgt aagagtccga caccttttga aaaattagat
     2581 tttgaaaaag attatccaga agcaggtgct tcaggtggtt ttatccacta ctgtgagtat
     2641 cctgttttgc aacaaaatcc aaaggccttg gaagcggttt gggactatgc ttatgatcgt
     2701 gtggggtatt tgggaaccaa tacgcctatt gataaatgct ataattgcca atttgaaggc
     2761 gattttaccc caacagaacg tggttttact tgcccaaact gtggcaataa tgaccctaaa
     2821 acagttgatg tggtcaaacg tacatgtggt tacttgggga atcctcaggc ccgcccaatg
     2881 gttaacggtc gccataagga aatctctgcg cgtgtaaaac atatgaatgg ttctactata
     2941 aaatacccag gcctgtaaac tcaagctggg ggagagccct tttgatggaa atatgatatg
     3001 cttaaaaggt tgcttcatgc aagcaatctt ttattgttta atggtttctt agcaattgtg
     3061 agataagaat aactaatcca aaacgactaa ttagctgtta gcaatcggac tataagaaga
     3121 aagagataag gaagaaataa tgggaaaata tcaattagac tataaaggaa tgcagcaggt
     3181 agaacgcttt catgagaagc attctaaaaa aaagacggat aaaaaatccc gcgttcaaga
     3241 acttaaggct cggtttttag agaagtcaaa aaaacaggat aactaaagta tccaataaag
     3301 aaagaaggtt gagtatgctt aatattggga ttgtgggact aggtgccatt tcacaaaaag
     3361 cctatttacc atatatgaga cagctgagtg acattacttg gcatctatca acgcggaatg
     3421 cagcagtacg tcagcaggtc ggtcagttat ttggtcacgc tattctttac agtgatgtca
     3481 aggagttgtc aaaggcaagt ttagatggcg tctttattca tgcggccaca tcggcccacg
     3541 cagagttggc tagtttattt ttaaatcagg ggatcccagt ctttatggat aaacctatag
     3601 ctgacaatta cctgatgaca aagaacctct atgacttagc caaagaaaat cagacttttc
     3661 tgatggcagg atttaacaga cgttttacac ctcgtgtcaa gaagttgtca agtttgtcaa
     3721 ctaagcgtaa agtggctgtt gaaaaaaatg acttgaaccg gccaggagat atgactttta
     3781 agctctttga tttctttatt catcctttag atacagcctt attcttgtca gaagggacct
     3841 tactaaaagg gcattttcaa tatcatctgg aagcaggctt actgagtcaa gttatggtaa
     3901 ccttgatgac cgaaagcatg acgactacag cttctatgaa tttacaatca gggagtcgcc
     3961 gtgaggtaat ggaagttcaa cgggctgaag aaacttatca tttagagaac ttagacgagt
     4021 tatctattta taaaggtact gaaaaaaggg tgcttggctt tgcttcttgg gacactacct
     4081 tgcacaaaag aggttttgaa acaatgatcg atgccttttt agaagctatt agcacaggcg
     4141 tcaatcctgt cagtcctgag tctagtcttt taagccactg gatttgtcaa cagattgctg
     4201 actctcagct ttcttatgga gagttaacgg tagagttgcc taaggattag gaattgatat
     4261 ggaaataaga cgaccgactt taaaagataa agacgctgta ttgtcaatga ttaacgaatt
     4321 tttagagcag aaaagtgcta cagatgggtt atggcatttt aacgtcaatg actttaacta
     4381 tgaaacatgg ttggaagatt ccttgcgaca ggaaatgggt ttgtccagtc aaggagtgcc
     4441 tgctattcaa tacgtcgcat ttgatgagag aagtcaggct atcgggttct taaatcttcg
     4501 tttacggtta aatgagagat tacttgaaaa aggtgggcat attggttact ctgtccgtcc
     4561 cagccagcgt gggaaaggtt atgccaagga gatgcttaaa caagctgtca gctatgcgat
     4621 ttccaagaat atcacgacaa ttttggtaac ttgtgatgag actaatgttg caagccgagc
     4681 agttattgtg gctaacggtg gtgtcttgga agatagtcga ggtggaacgg aacgttattg
     4741 gattgaagaa caaagaggcg ataagtgatg gcagaaaagt gttggaataa tcctaagcca
     4801 aaggaatggc aggcagagga gttaagtcaa ggccggatta ttgactataa ggcttttaac
     4861 tttgttgatg gtgaaggagt tcgtaattcc ctttatgtgt ctggttgttt attccattgc
     4921 aaggggtgtt ataatgctgc gacttggtct tttaaggcgg gaatgcctta tacgcaagaa
     4981 cttgaagagc agataatgac ggatttggca cagccttatg ttcagggact gacactttta
     5041 ggtggagaac cctttttgaa tacgggtatc ttaattcctc tgattaagcg tattcggcgt
     5101 gaattgccag aaaaagatat ttggtcttgg acgggttata cttgggaaga aatgatgctt
     5161 gaaacaccag acaaactgga aatgctgtcc ttaattgata ttctggtaga tggtcgtttt
     5221 gatatcacaa aaaagaacct catgttgcaa tttagaggct cttctaacca gcgaattatc
     5281 gatgttcaaa agtctttggc tgctaaagaa gtgattatct gggataagtt aaacgatggg
     5341 gaccaaacct ttgaacaaat tagtcgggag gatttgcttt aagaagctga cttatccaca
     5401 tgattttttt gaaaaggtca tgtgaaaaca ggtcctttaa gggtgaattg gggagaaagt
     5461 aattgttatt tcctggtttt ccccacatta tcctcatagt tatccacagg ttgtgtataa
     5521 gttgtagaat tgtgaataaa gactaaaaaa gaggcctagg cctctttttt gatatagaca
     5581 aattcatgat ctgttgatag gtctggatgg tagtaaaaac cagcaaagac aaggctttta
     5641 aggctgtcaa cagtttggca attattttcc atacaggcag tgagaaatgc acctcgtgcc
     5701 ttttttgaaa tggtggaatg ggttttgaat tggccctctt tttctgccat aaacttggga
     5761 cttatccaca attgtttaca gtctttggaa aagacatcgt caaattcact ggataaaagg
     5821 gaaatcactt ggggatgctc tttggcaaac tgattataac agggccgcca gtaagacttg
     5881 aggctttgtc cttcaatttt tattctggtg tgaaaatcat ggcggtgctc tgcgatagga
     5941 tgattagcgg gaatgatacc gtaaaaggat gaggtgatgt aaacttgctg ggtgaggtaa
     6001 gcctgttctt gggtagtgag cttatcgcgc ttgatatggc ggtacataag gccattgaac
     6061 agttggtagg cagggtaagc caagttttgt tgagaggcca tgtcttgcca gcgttgctgt
     6121 tcttttttag ctgcctcttc tttgatacga taggatttgg ccaagtcctc cgtggtcata
     6181 gctgccatga tttttaaaat ggcttggctg tcttgtggaa ggaggtgtgg gtgagattcc
     6241 ttagggatca tcatttcttt tgcggttgga attaaaaagg ttagcatagt gttattgtag
     6301 taaagttact tgctttctgt caagaaaact agtcttgtcc aaggatgacg ttcacacaga
     6361 taccttcctc gtcttctgtc gtgaaagaag aagaatcttc ttgtaaaata gctaggccgt
     6421 gtaatcttgc ttttttcaga gtagctgaaa aaagcagggg cgtctcaatg tgaatggtca
     6481 agaaggctaa gcctggggca ccttcttggt gttttttgag gtaaggggct gaccagtgat
     6541 tgaatgcaag gtgatgatgg tagttccctg aagcaatcca gctagcactt ggaatggtca
     6601 ttttatcgcc taaatcaaaa accttttgat agagaagtga agaagccaga gcatttttga
     6661 cactcagatg gacatggcca atgcgtgtgt cttgagctag taaaaagcgc ttggggatat
     6721 ctgtcaactg ctccaatatg cttttggtat ccgtgggctc agtcacacca ataatttgac
     6781 cattgtcgcg gatatcccaa tgctcaacag ctttgtcatg ataaatttca atcccattgc
     6841 cctcaggacc gctgaggtag atagcttcgc tatagccgtg atcagctgat ccttctaaag
     6901 agatacctct ggttaagaaa tgattgagaa caagtcccaa gctatgacga tcaggaacta
     6961 aaaaggatgt gtgataaaga ccataagcct tatttgctgg taaaggtgtt tgtcgtaact
     7021 ctaagataac ggttttaccg tctgtagtca attgacgaga tgtcgtgtct tgtgagagaa
     7081 cctgtaaacc gataatactt gtgtaaaagg ttgtcatctt tgctagatcg gtaacattta
     7141 gagaaaccgt tcctaaagag atggttgagt tgtaagggta tgtcatccta ttacctccaa
     7201 aaactaatta aatctatcat aatcaatcag tttcaaaaag aaaagtaggc acaaataagt
     7261 ggtataggca ctaaaaagta actcatttgt cagccctatc ttgtttctgt tctctctaat
     7321 agtctataat gggttataag ttacaaaaag atactaagag gagctgctgc ctatgctgaa
     7381 aaaaagaccg gcttgtcccg tagaaacaac cttatcggta atcgggaata aatagaaatt
     7441 attaatatta agggacttgc taaagggacc ttgcgttttg ggcagttgaa gtcctcaata
     7501 ggtttggtca gtcaaaaggt acttactgca caattaaggg ctatggaagc agacggattg
     7561 gttcatcggg aagtgtatgc agaagtgcca cctcgggtgg agtattcctt aacagaaact
     7621 gggttgagct tagctcctgt tattgaagcg atgtcggatt ggggccaaac ttaccaagaa
     7681 aaacatgccg atttatttga agaaggctag aggaaagagt tgtccgagca tactgagact
     7741 tagagaagct gatagttttt aaaaaatgat attatcaaat tctttacagt ttgataaaat
     7801 tacttttttt ctacaaaaag acttgcgctc atctcacgaa tatgataata taatatcaag
     7861 tatgtttgag aaaacagaag ctcaggtcaa gtaagtgcac acttacaata acacagttga
     7921 taatggggaa actcagagtc aagaaaaaga aaagcaagcc ttgcattttg caaggctttt
     7981 ttctgtcttt attttggtct gcgccgagca aaatctaaaa atttaaattt ttccagttta
     8041 tggcgactct cggtgaattg gaattggtta ttattggaaa ggtagacttt ggatttgacg
     8101 gaaacaacat gttggtctga tccaaggtca agtaaaattt tatcacgatc ggttatttgg
     8161 tcaatggtaa cctctttgag ggcaaagtca atggcgagat ggagttcctt ttccaaatat
     8221 tgataaatag aatgctctgc gatttccctt gtcattattg gcaccaaagt tttactaagg
     8281 taatcaatgt ctaagacgga agccacaccc tcaacaacgc gttggcgtgt aacacgccag
     8341 accaagctgt ttttgctaaa accggtcaat tttgaaagtg tctcatccac aatcagttta
     8401 tcaatggcaa tgacgttggt cttagaatct agatttgagt aagagacgag ttcctgataa
     8461 ctagttagtt ctgagatagg gaacatgatc tgatgatgtt taatcacttg ggtgccacgt
     8521 ccttgttttt tgaggataag acctgccttt gtgagaagtg agagagcctt gcggacggta
     8581 tctcggctag cttggtattg ttgactcaat tctatttcag taggtaaaaa atccccttct
     8641 ttgtaaaagt ctttgtttat ttttgtttcg aggtctttat aaatacgttc atactttgtc
     8701 atagggtaaa ttacctttcc atttgtctat ttttttacag acaacacctt ttattttacc
     8761 aaaataatgg cttttttggc acaaacaact tgtagacaaa tttgcaatcg attacaataa
     8821 aggtgtcagt tagattctat ctaagaattt gaatatttca ttttgacgtt gcttgttaaa
     8881 agcaactaga acaaaggact aggggatgtc acatgggaaa atttgaacag gatgctaaga
     8941 gtcttctaac tgctattggc ggtaaagaaa acatcaaggc tgtcacacac tgtgcaacgc
     9001 gtatgcgttt tgttttgaat gataataata aggcaaatgt caaagagatt gaaaaaatct
     9061 ctgtagttaa agggacattt accaatgctg ggcagtttca ggtaatcatt ggtaatgatg
     9121 ttccagtttt ttataatgac tttacagctg tttctggtat tgaaggggtg tctaaagaag
     9181 ctgccaaatc agcagctaaa agtaatcaaa atgccttaca acaggtgatg accatgttgg
     9241 ctgagatttt cacacctatt attccggcga ttattgttgg ggggcttatt ttaggtttcc
     9301 gtaatatttt ggagagtgtg ccttttgaat ttcttgggca gcaggtcgaa aaagggaaat
     9361 tagtttttga tgcagctggg gatcctgttt ggaatacgat tgtgagggta tctcctttct
     9421 ggtcaggggt taaccatttc ttgtggttac caggggaagc tattttccac ttcttaccag
     9481 ttgggattac ttggtctgtg acgcgtaaga tgggaaccac tcaaatttta gggactgtcc
     9541 ttggtatctg tttggtgtca ccacaattat tgaatgctta tgcggtagca ggaacgcctg
     9601 ctgctgagat tgccaaaaac tgggtttggg attttggttt ctttaccatt aatcgtattg
     9661 ggtatcaggc acaggttatt ccagcccttt tagctggtct gtcccttgct tatcttgaaa
     9721 ttttctggcg taaacggatt ccagaagtgg tctcaatgat ttttgtgcca ttcctttctt
     9781 tgattccagc tttgatttta gcgcatacgg tattggggcc aatcggttgg actattggta
     9841 aagggatttc ctttgttgtg ttagctggtt tgactggtcc tgttaaatgg ctattcggtg
     9901 ctatctttgg tgccttgtat gctccgctag ttattactgg tttacatcac atgacaaatg
     9961 ccattgatac ccaattaatt gctgatactg caactcgtac aactggtttg tggccaatga
    10021 ttgctctttc aaatatcgct caagggtcag ccgtttttgc ttattattta atgaatcgtc
    10081 atgaagaacg tgaggctgaa atatcgcttc ctgcagcaat ttctgcttac cttggggtaa
    10141 ctgagcctgc cttatttggg gttaatgtta aatacgttta tccctttgta gccggaatga
    10201 tcggatcagg tattgcgggt ctcttatcaa caacctttaa tgttcaggca aattctattg
    10261 gtgttggtgg cttaccaggt ttcatggcta tcaatgtgaa gtacatgatt ccattcttca
    10321 tctgtatggt agtagccatt gtggtgccga tgtttttagc cttctttttc cgtaaatcac
    10381 atatcatgac taagacagaa gatgaagcta aactacctga gacacccgtt tcggatgctc
    10441 ctgtagcaac tgctccacat aagactatgc aaggaacagt tatcacttta acaagccctt
    10501 taacgggtga agttaaagcg ttgtctgaag ctgttgatcc tgtctttgca cagggagtta
    10561 tgggccaagg tgctcttctt caaccgacag aaggggtgtt agtagcgcct tgtgatgctg
    10621 aagtatcggt cttgttccca actaaacacg ctatttgttt ggtgacgact gaaggtttgg
    10681 aattattgat gcatattggc atggatacgg ttaacttaga tggtcaagga tttgaagctt
    10741 tggtgaagca aggtgatcag gttaaggctg gacaaacatt gattcaattt gatatagcag
    10801 ctatttctga agctggatac gccactgaaa cgcctcttgt ggtgactaat caagatgctt
    10861 ttacggtaac tgttgaaggt agtttaccgc gtcagattaa ggctaatgat aagttagcag
    10921 tagcgatgaa aaagtaggtt tactagggag aaaacgttca ggaactgaga tcagttcctg
    10981 aaactatttt agggaggaaa actcatgaca attgataaaa agaaagtcgt ctatcaaatt
    11041 tacccaaaat cctataagga cacgactgga aatggtgtgg gagacttgcg agggatcatt
    11101 gataaattgc cttacttaca agaactagga atagatatga tttggttgaa ccctttctac
    11161 cctagtccac aacgagacaa tggctatgat gtttcagatt atacggcggt caaccctgat
    11221 tttgggacaa tggctgattt tgaagatttg gtaaaagctg ctaaggagca tcagattgag
    11281 ttaatgttgg atatggtttt gaatcactgt tccacagacc acgagtggtt ccaaaaagct
    11341 ttagcaggag acccttatta tcaggatttc tttatcttga gagatcagcc gactgattgg
    11401 gtttccaaat ttggtgggaa tgcttgggcg ccttttggag atacaggcaa atactactta
    11461 cacttgtttg atgtgacaca ggctgacttg aattggcgga acccacatgt tcgtgaggaa
    11521 ttggctaaag tggttaattt ttggcgagat aaaggagtga agggattccg gtttgatgtg
    11581 attaatctga ttgggaaaga tgaagagctg gtggattgtc cggtcaatga tggtaagcca
    11641 gcttatacgg atcgtcctat tactcacact tatcttcatg atctcaatca agccagtttt
    11701 ggtcaggatg attcgtttat gacagtaggg gaaatgtctg ccacgactat tgataactgt
    11761 cttttataca cggctcccga acgtgaggag ctatccatgg cttttaattt ccaccatcta
    11821 aaagttgatt atgagaacgg tcagaaatgg actattatgg cttttgattt tgcagcgctg
    11881 cgagacttat tccatgcttg gggtgaaggc atgagtcagg gcaatgggtg gaatgccttg
    11941 ttctacaata accatgatca accacgtgcc ctgaatcgtt ttgttgatgt aacacatttc
    12001 cgaaacgaag gtgcgacgat gttagcggct tccatccatt tgtcacgagg aacgccttac
    12061 atttatatgg gtgaggagat tggcatgctt gatccagatt ttgatagtat ggatgattat
    12121 gtggatgtgg aaagtctcaa tgcttactca agcttattag tctcaggtaa aagtgcagaa
    12181 gaagcctttg ccattatcaa ggctaagtca agggacaatg ccagaacacc aatgcaatgg
    12241 gatgctagtg aacatgctgg ctttacgact ggtaagcctt ggttagaggt tggcaaatct
    12301 tatcgagaca tcaatgtcga aacagaaaaa gagggacgta ttttcccttt ctaccaacgc
    12361 ttgattgctt tgcggaagga actccctatt attgctgaag gggactatcg ggctgctttt
    12421 aaagatagtc aggctgtcta cgcctttgaa cgccatttag gtgaccagtg tttgcttgtt
    12481 ctcaatcatt tctatgctgg tgaggtcgaa ctggaattac ctccacgtta tcaacatgga
    12541 caggccttaa tcagcaacta tgagaaagtc tctatttgtg aaaaagtgat gctgaaacct
    12601 tatcagacac ttgctatttt agctgataac taactaaaag cctgaggtta ggcctcaggc
    12661 ttttggtgat tgtcacgaaa aaagcagttt tctcatatat ttatttttaa actcttattt
    12721 tttctaaata aaaacaattg agataagtta ttttagctaa attttggtac aatggaaaag
    12781 attataagta gagtttaaga taaagattct acaaatttaa aatgatataa ggagatacga
    12841 atgacaactt atcaagatga tttttaccag gctgtgaatg gaaagtgggc tgaaacagcg
    12901 gttattccag acgacaagcc tcgaacaggt ggattttcag acttagcaga tgaaattgag
    12961 gcattaatgc tagacactac agatgcttgg ttagcagggg atcatatccc tgatgatgct
    13021 attttaaaaa attttgtgaa attccatcgt ttggtggctg actacgctaa acgtgatgaa
    13081 gttggagtaa gtccaattct ccctttaatt gaagaatatc agtccttaaa atctttttca
    13141 gaatttgttg ctaatatagc gaaatatgaa ttggcaggtt taccaaatga atttcccttt
    13201 agtgtggcac cagattttat gaatgctcag ctgaatgtgc tttgggcaga agcaccaagc
    13261 attcttttgc cagatacaac atactacgaa gaaggcaacg aaaaagctga ggaattgcgt
    13321 ggtatttggc gtcagtcgca agaaaagctt ttacctcaat ttggtttttc aacagaagaa
    13381 atcaaggatc ttttggataa ggtaattgag ctagataagc aattggccaa gtatgtcttg
    13441 tctcgtgagg aaggctcaga atatgccaaa ttataccatc cttatgtgtg ggctgatttt
    13501 aaaaaactag ctccagagct tcctttggat agcatttttg aaaagatttt aggccaagta
    13561 cctgacaagg ttattgttcc tgaggaacgt ttttggacag aatttgcagc cacttattac
    13621 tctgaagcca attgggattt actcaaggct aatttgattg tggacgcggc taatgcttac
    13681 aatgcttact tgacggatga tattcgtgtg gaatcaggcg cttattcgcg cgctctttca
    13741 ggaacacctc aagctatgga caaacaaaaa gcagcctttt atttggcgca aggaccgttt
    13801 agccaagcgc ttggcttgtg gtatgctggt caaaaattct ccccagaagc taaggctgat
    13861 gtggaaagta aggtagcacg catgattgag gtttataagt ctcgtctaga aacggctgat
    13921 tggttagcac cagccacacg tgaaaaagcc attactaaat taaatgttat cacgccacat
    13981 attggttacc cagaaaaatt accagaaacc tatgctaaaa aagtgattga tgagagcttg
    14041 tcattggttg aaaatgcaca gaacttggct aaaatcacga ttgctcacac ttggagtaaa
    14101 tg
//
LOCUS       DAUVUY010000050        14070 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.050, whole genome shotgun sequence.
ACCESSION   DAUVUY010000050 DAUVUY010000000
VERSION     DAUVUY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14070
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.050"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(70..294)
                     /locus_tag="VOX58_001222"
     CDS             complement(70..294)
                     /locus_tag="VOX58_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HES5878451.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            complement(355..1329)
                     /locus_tag="VOX58_001223"
     CDS             complement(355..1329)
                     /locus_tag="VOX58_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HES5878452.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            complement(1330..1764)
                     /locus_tag="VOX58_001224"
     CDS             complement(1330..1764)
                     /locus_tag="VOX58_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HES5878453.1"
                     /translation="MEYDKINQYLVDIFNRILVIEEMSLKTSQFNDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            complement(1841..2632)
                     /locus_tag="VOX58_001225"
     CDS             complement(1841..2632)
                     /locus_tag="VOX58_001225"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HES5878454.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            complement(2852..3988)
                     /gene="dnaJ"
                     /locus_tag="VOX58_001226"
     CDS             complement(2852..3988)
                     /gene="dnaJ"
                     /locus_tag="VOX58_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HES5878455.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            complement(4269..6095)
                     /gene="dnaK"
                     /locus_tag="VOX58_001227"
     CDS             complement(4269..6095)
                     /gene="dnaK"
                     /locus_tag="VOX58_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HES5878456.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAVAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            complement(6276..6848)
                     /gene="grpE"
                     /locus_tag="VOX58_001228"
     CDS             complement(6276..6848)
                     /gene="grpE"
                     /locus_tag="VOX58_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HES5878457.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(6851..7885)
                     /gene="hrcA"
                     /locus_tag="VOX58_001229"
     CDS             complement(6851..7885)
                     /gene="hrcA"
                     /locus_tag="VOX58_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HES5878458.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKTFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDSSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(8020..8589)
                     /locus_tag="VOX58_001230"
     CDS             complement(8020..8589)
                     /locus_tag="VOX58_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HES5878459.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            complement(8586..9329)
                     /locus_tag="VOX58_001231"
     CDS             complement(8586..9329)
                     /locus_tag="VOX58_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HES5878460.1"
                     /translation="MKHNHFKLLLQTLVVLLFVGGIYLFIKPEKQATKTPSSQQASSQ
                     KVVKNSKESTGLPKVSPKDWELILVNRDHMTEELSPELVDINGISVDKRIEQATSDFL
                     AAAQAIDPQEHLISGYRSVAYQAELYQSYIKKEMANDPTLTQEAAEALVQTYLQPPGA
                     SEHHTGLAIDMSTVDTLNASDPSVSKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(9319..10026)
                     /locus_tag="VOX58_001232"
     CDS             complement(9319..10026)
                     /locus_tag="VOX58_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HES5878461.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEVDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFMWLIDPDREKQYIDNGSVTVVEFEDGQFTIKTIGDM
                     SYRYRGREIIEAMSDEA"
     gene            complement(10418..10657)
                     /locus_tag="VOX58_001233"
     CDS             complement(10418..10657)
                     /locus_tag="VOX58_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HES5878462.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(10876..11091)
                     /locus_tag="VOX58_001234"
     CDS             complement(10876..11091)
                     /locus_tag="VOX58_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878463.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTVYKKTIIYAKI
                     RLTGIALEPSGVSDVYLMNYHVCLHYY"
     gene            complement(11088..12527)
                     /gene="gatB"
                     /locus_tag="VOX58_001235"
     CDS             complement(11088..12527)
                     /gene="gatB"
                     /locus_tag="VOX58_001235"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HES5878464.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIRLTPDNLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            complement(12527..13993)
                     /gene="gatA"
                     /locus_tag="VOX58_001236"
     CDS             complement(12527..13993)
                     /gene="gatA"
                     /locus_tag="VOX58_001236"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HES5878465.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(13993..>14070)
                     /locus_tag="VOX58_001237"
     CDS             complement(13993..>14070)
                     /locus_tag="VOX58_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HES5878466.1"
                     /translation="LFKNVPEKENHFIKVPAILDDGGDA"
BASE COUNT         4113 a         3088 c         2467 g         4402 t
ORIGIN      
        1 agtgatgaac atgaacataa cagcagtaag cttttcatag acttctgatg ttacttgtca
       61 cttttatcct tattttgact tttcctcaac gtaagcaaca aggtcaccaa cagtatttaa
      121 tccttcttct gtttcaattt gaatatcaaa agcatcttca atttctgaaa taacttggaa
      181 aacatcaagt gaatctgcat ctaaatcatc aaatgtagtt tctagcgtta cttcttctgt
      241 ctccttacca agttcttcaa caataatttc ttgtactttt tcaaatactg ccatgtttat
      301 ttctccttaa aaaatataaa tttgttttta taaatgtgct aggcacatga ataactagat
      361 tttaacaatt agacttcccc atgtcaaacc tccaccaaaa ccggaaagta agattttttg
      421 ggtaccatct aacctaatct gtcccttttg aacagcctct gacaagagaa ttgggatact
      481 agcagcacta gtattaccat aacgcatcat gttttcaagg aatttttccc gaggaacatc
      541 aatcttgcgt gctattttat caagtatacg acgattagct tgatgcaata aacaatagtc
      601 tatgtcatct tttgtaatat ctgattgcgc cattagtgtg aggatgcttt ttgacacatc
      661 cctaatcgca aaatcaaata tggcacgtcc atccatctga atagtcgcta tggctttttt
      721 gccaattgag tacggagatg aaagactagt ttctcctgaa attaaactct gacatctcgc
      781 accatctgtg tgtaatgttt ccgctaaaac atgtttatct ttactggctt ccagtagcac
      841 gccaccagct ccgtctccaa aaagaacggc tgtcgctcga tcctgccagt ttactaactt
      901 agataaagtc tctgcaccta ttaccattcc gttttggtag gcacctgatg caataagctt
      961 atctgccata gccaaggcaa agacaaagcc actacaagca gcagtcatat caaaagcaaa
     1021 agcacttgta gccgcaatag ctgcttgaac tttcgcagct gttgatggca tagtggcatc
     1081 agggctgata gttgcaacaa taataaaatc aatagcatca gcttttaacc cagactgagt
     1141 taaaagctga tcagcaacct gaatagctag gtcactagtc atttcatctc tagaaatatg
     1201 gcgttctgct attcctgttc gagaaaagat ccactcatga ctagtgtcca ttatgctagc
     1261 taaatcatta ttagtcacta actgctgtgg aacatagtgg gctacctgac tgattttaga
     1321 aaaaatcatt taaaccaaat cctccaaaaa ttgatgtaga ttccctagtc cacgcaccaa
     1381 tgcctgcatc tcttcatcac tcatatcagc aatgacatgt ccaaccatat ttttatggaa
     1441 ctttgcatgg aggcggtcca aaagtcgacc cctttttgtt aatgacaggt aaacaactcg
     1501 cctatctgag cgtgatcgcg ttcttgcgat ataacctttt gcttctaatt tatttaagct
     1561 agtcgttact gtccctaacg ttaccattaa ttctctggcg atatcacttg gagtcacctg
     1621 atcatatttg ccgataattt caatagtgtg catctctttt aaagagacat cattaaactg
     1681 actcgtcttt aagctcattt cttcaataac caaaatccgg ttgaaaatat caactaagta
     1741 ttggttaatt ttgtcatatt ccaagtaaac accttctagc aataaacttt gacaatcaaa
     1801 ttatatcaaa ggtcaaagta ttttgcaagc aacagctgtc ttacttacct tgaaaatttg
     1861 gtcggcgcct ttctccaaaa gctatgacac cttctttaaa atcttccttg aaagctagct
     1921 cctcttgaat agcaagctct gccttagcat aatcttccca tcctgtgaaa aagctttgcc
     1981 agaccaatga tttcattcca gcataagaat ttgaagagcc acgtctcaat cgctttaaaa
     2041 gttgaagaca aactttgtct agtttatcag attctgccgt acggtagaca aacccataat
     2101 cgagaccttt atcagcagta attccttcac ctgtcatcac aaggtgagtt gcacgattaa
     2161 gtcctactgc acgagtcaat agaaataaac caccggcatc cggtgctagt ccaacattta
     2221 caaaagcttg gataaatttt gtttgagtac tagcgataca aaagtcaaca gctaaagcta
     2281 tattaaaagc cgctccagct acagctccat cggcgcaaag gatgactggt tttggcaaat
     2341 gcttgattgc aaaagaaatt tcttgtacca gttcagcaat cttgactagc gactgcacat
     2401 tatcttttgc aacagcttct tgcatttcaa ctaggtcacc accaactgaa aacacttttc
     2461 caacggcttt aattaataaa aaacgcacag atgtatcgcg tttaacttct gctaatgcta
     2521 ccagaatctc ttgacaaatt ggaatattaa aaccgtttga aacctctgga cggtttaaag
     2581 tcaatgtcgc aagatcatct acaacatcaa agatgatgtg tttaaactgc ataacaatcc
     2641 tcttttctga taatttattt gactaagaaa ttatttctct ttaaaatcat ttgataatca
     2701 aatcaattac ctaattatga tatactaaat aaaccatttt gacaagagaa aaaaacgata
     2761 tctataaaag aaaacgtttt ctctaactgt tttgacgtaa aaaaacgtat aacttcggga
     2821 aaattatcct gagattatac gtttgacatc tttaaatatc ctctaaggca tctttcactt
     2881 tatcaaaaaa accttttttc tttggatgta acattttttc accactcgct tctgcaaatg
     2941 cttgcaaggc ttccctttga gcgtcattga gtttggttgg ggtcacaata ttaacggtaa
     3001 catgttgatc cccttgacct ccaccacgga gttttggtgc tcctttgcct ttgaggcgga
     3061 aggttttacc tgtttgtgtc ccagcaggga tagccatctc aacatcgcca tgaaccgttg
     3121 gaatttcaac agtatctcct aaggctgctt gagtaaaaga aatatctaag ttgtagtaaa
     3181 tagtggaacc attgcgttca aattgcttac ttggtaagac attcaaaata acaaataaat
     3241 caccataagg tccgccatta aagccagctt ctccttgacc ttgtaaacgg atttgctgac
     3301 cagtctctac accagctgga atcttaacag acactttatg agcttgcttt tcatgaccag
     3361 taccgtgaca ggtttgacag ggttctttaa tttccttacc actaccatga caaatatcgc
     3421 aggtgacttg tcgacgcatc ataccaagtg gtgtttgggt gtcaattgtc attacacctg
     3481 aaccatggca cttacgacaa gttactggag cggtaccagg ttttgcacct gagcctaaac
     3541 aagtgccgca ggttgcttcg cgattatagc taacttcttt ttcaacacca aatacagcct
     3601 cttcaaaaga taaattaaca cgatattgca aatcgtcacc ttgacgaggg gcatttgggt
     3661 ttcggcttcc accgccacca aagaagcttg agaagatatc ttcaaaacca ccaaagccac
     3721 ctccgtcaaa accaccaaag ccgcctgctc cgccaccaaa gccgccttga gcacctgcag
     3781 cgccgtattg atcataagca gcacgctttt gtgaatcact taaggtttcg taagcttctt
     3841 gaacatcctt atacttttgc tccgctccag cttctttatt gatatctgga tgatatttct
     3901 tagacatttt tcgataagct tttttgatat cgtcctgaga agcgtcctta gaaaccccca
     3961 gacgatcgta atattctgta ttgttcataa tgtaagatac caagccgtaa aacgactcaa
     4021 tgaaaaatag gaaatccgac aaagatactc tcgtatctag gaggtatttc tcttttttcg
     4081 cagagtcgta ggcgtgttcg aatccgcaaa agagtcttta cacgctttag cctgtgttcg
     4141 aataatcgaa cttgtattcc tttcttaaaa tattttgacg tcagcatttt tacattttat
     4201 ttagccaaaa acagaggttt ggtacaacct ctgaattcgg atattaatgt tactagataa
     4261 ctaaatcatt acttttctgt aaattcgcca tctacaacat catcattatt tgctgaatca
     4321 ttagcttgtg caccttctgc accttgtgct gcttgttgag ctgctacagc ttgctcgtac
     4381 attttaacag ccaaagcttg cgctttttca tttaatgctt caagtttagc tttcatgtcg
     4441 tcaaggttgc cagattcttg cgcagctttt aactcgtcaa gagctgattg cgctgcatcg
     4501 cgttctgtat caaagccttt accttcagtt tctttgattg ttttttcagt agcaaagata
     4561 gcttggtcaa cttcgttttt aaggtcaact tcttctttac gtttcgcatc ggcttcggca
     4621 ttagcttcag cgtctttcat catgcgatca atttcttctt cagaaagtcc gtcgtttgat
     4681 ttgataacga tgtgttgttc cttttgcgta ccaaggtctt tagcttttac agaaacaata
     4741 ccgtttttat cgatatcaaa tgttacttca atttgtggga ttccacgagg tgcagctggg
     4801 atatcagtca attggaagcg accaagagtc ttgttatctg ctgccattgg gcgttcacct
     4861 tgaagaacat ggatatcaac agctggttgg ttgtctgctg ctgttgagaa gacttgtgat
     4921 ttagatgttg ggatagtggt gttgcggtcg atcaatttgg tgaagacacc acccattgtt
     4981 tcaataccaa gtgacaatgg tgttacgtca agaaggacaa cgtctttcac atccccagtg
     5041 ataacaccac cttggatagc agcacccata gcaaccactt catcagggtt tacagattta
     5101 tttggttctt taccagtttc agcttttaca gcttcgacaa ctgctgggat acgagttgat
     5161 ccaccaacaa ggataacttc atcaatttct gacaatgaca atcctgcatc tgaaagggct
     5221 tgacgaactg gagttttcgt acgttctaca aggtcgcgag tgagatcgtc aaatttagca
     5281 cgagataagc tcatctccaa gtgaagagga ccggcagaac cagcagtgat gaacggtaat
     5341 gaaatttgtg tttgtgtcac acctgaaaga tcttttttag ctttttcagc agcatctttc
     5401 aagcgttgaa gtgccatctt atcttgtgat aagtcaatgc cattttcttt cttaaattca
     5461 gccactaaga aatcaataat tttttggtca aagtcgtcac caccaagttt gttatcacct
     5521 gctgttgcaa gaacgtcgaa gacaccatca cctaattcaa ggattgatac gtcaaatgta
     5581 ccaccaccaa ggtcaaaaac taagattttt tcatccttgt cagtcttgtc cataccataa
     5641 gcaagtgcgg ctgctgttgg ttcgttaacg atacgttcta cttcaagacc tgcaatttta
     5701 ccagcgtctt tagttgcctg acgttgtgca tcgttgaagt aagctggaac agtaataact
     5761 gctttttcta ctttttctcc aagatagtct tcagcataac ctttaaggta ttgaagaatc
     5821 attgctgaaa tttcttgagg agtatattct ttaccatttg cagaaacttt ttcagaagtt
     5881 cccattttag atttaataga gattactgtt tctgggtttg tcactgcttg acgttttgca
     5941 gcatcaccca cgataatttc accatttttg aatgatacta ctgaaggagt tgtacgattg
     6001 ccttctgggt tagcaatgat ttttgattca gtcccttcaa gaactgctac tgctgagttt
     6061 gttgtaccta agtcaatacc aataatttta gacatgttaa tacctcttat tttataatac
     6121 tttattactt tttatttcaa tttatcttag taagcactcg aattatttta cttccaattc
     6181 taagcaatca ctatagttta atcagcaagc gaaataactt ctaacttgct atctagttta
     6241 ctgtcattac ggacaaaaac gaaaactcat ttaccttagt tatagacaac taccatcgct
     6301 ggtcttagca gacgctcgtg caatttatat cccttttgaa agacttctgc aatgctatcg
     6361 gctggatgtt catcatcggc tgggagggtc tgaactgcca tatgaaagtt gtggtcaaat
     6421 gagtcaactt ctacttcttc tacgccttct tcctttagtg cttgaataag gctatcacga
     6481 gtcatttcta aacctttttt aacatcatct gtcaaccctt caacagccaa ggcacgttct
     6541 agattgtcaa gcgatggtaa gattgctttt gccagatcct gagaacgata gcgttgaagt
     6601 tgttgacgtt cttcactaga gcgacgctgt atattctgca tttctgcatg agctcgcaag
     6661 tatttatttt caaactcatc agcacgttcg tttgcaagtt ctaactctga tttttcagga
     6721 gtttcttcaa taacttcctc aactgtctct tcagtttcag tggcttccac ttcttcttgt
     6781 aaatcgtctt ttttaatttc ttctgacact gagtgcctcc ttttaaataa cttattgaat
     6841 actgccatta tcagtggact tcataatggt tagaatttag gtatcgataa taatcaccta
     6901 atttagcagc caataccttt ccaataatgt ttaccaaact aacagaccta cgataatcca
     6961 tatcaatagg accaatcaaa ctcaggagtc caaacccacg gtaaggaatc aaaaatttgt
     7021 gagtcaaaac tgacacatct gcaagtgcgg cttcttgact atctgccact tggacagaag
     7081 ccatctcacc ttctttcaga ctttgcctca aagaaattgc tacttgctgt tcattatcca
     7141 aaaactgata cgttgataga tctgaatacg ttaaagaatt taccttacca gcaacaaata
     7201 ccgtttccaa aaacagctct gaaaaaacat aatcaaatag ttgtaaaaca ttatccgtag
     7261 tcacaaaata cttttgaacg atctgaggaa tttctgtcct caacttataa tgaatatcta
     7321 tgacactact atccagcaag cgctcctcta cgatagcttt aaatgctatc aagtcacgtg
     7381 tcaaaaagtt tcttggaata gcaaactgca ctgtcactgg cttagattca tctaaagtca
     7441 tgactgctag agcatcatgg ttgctaagct gaacaacatc aaaccctgtt aaacgttgac
     7501 gagctggctc cacatctaaa ataacggatg tatagcccgt catctccgct aaaatgtgac
     7561 tagctttttg aagcatgtct tctaatttga atgcttcaaa atcaaaagtt tttatgacat
     7621 gataaatatc ttgctcatca atactatcta gcctgaggga gtgctctaca aaatacttaa
     7681 agccagctgg acttggcatc cgtccactag aagtgtgcgc tttttccaac aatcccaact
     7741 tctctagttt tgccatatca ttacgaatag tggcgctact agaatcaatc gttctctgta
     7801 aagctttaga ccctactggt tcatgagttt gtgtaaatag ctcaacgatt aaattcaaaa
     7861 tatcattttg acgttgtgta atcacaaacc aaccactccc tttcgtttag cactctattt
     7921 agtagactgc taatttataa ttctattata ctctttatgg gacacatgtc aagaaaaaaa
     7981 ctcaaaaaaa ttagcactct tttataaaga gtgctaattt cagtcatagt ttgttaaatc
     8041 gttactttca atgacctgaa tcagtcgcct agcataggtt ttatcagtat aatgcaaata
     8101 cttttgtaaa cttttagctg gtgctttata ccctttttct gtagcaagat tacgatacaa
     8161 acgcttatcg acttcttttc cctgccgaag cattcgtaaa taatctctaa ttgcactagc
     8221 gtcatcttta taaatagcat atctcacgtt ttggttggtt tgtttagggg actttagggt
     8281 aatggccgct tgtcctggag ttgctttctt gctaaaaaga ttatgatact tagacgctag
     8341 taatgttttt ccatcatggg tttccaaaag aatttgagca atcaaaatag atggtctaat
     8401 gccataatat tttgccaatg gtttaacttc tttacccatt tttgtaataa attgtttttg
     8461 gctgtagttc acccttactt ctttatctgc actagctatc ctttggctac tcagtaaggg
     8521 acaaacaatc agcaataaaa agcaagctag aagcgttaaa aaatacggaa actttagtcg
     8581 ttttctcatc gtttctcctt taaagcagca atgtactctt ccaacgttag gttgtgctga
     8641 gccatataac gagcagacgc cttgcctaca tagcggtaat gccaatcctc ataatcaacc
     8701 cctgtactag tcttttttcc ttctggaaag cgcaagacaa acccataatc aggtgcaatt
     8761 ttttgaactg ctttagatac cgatgggtca ctggcattta aagtatctac agtgctcatg
     8821 tcaatagcaa gaccagtgtg atgttcacta gcccctggcg gctgtaaata ggtttgcacc
     8881 aaagcctccg cagcttcttg tgtcaatgtt ggatcgttag ccatctcttt tttaatataa
     8941 gattggtaaa gctctgcttg gtaagctacg gagcgatagc ctgaaataag gtgctcttgt
     9001 gggtcaatag cctgagctgc cgctaaaaaa tcactagtgg cttgctcaat gcgtttatcc
     9061 acagaaatac cattaatgtc aactaattct gggctcaatt cctcagtcat atggtcccga
     9121 ttcactaaaa tcaattccca atcttttgga gagactttag gaagccctgt ggactctttg
     9181 ctatttttga ccactttttg actgcttgcc tgttgacttg aaggcgtctt ggtggcctgc
     9241 ttctctggct taataaagag gtaaatacct ccgacaaaga gcagaacaac cagtgtttgc
     9301 aataataatt taaaatgatt atgcttcatc tgacattgct tctattattt cacggccacg
     9361 gtagcgataa ctcatatctc caatggtttt gatggtaaat tgcccatctt caaattcgac
     9421 aacagtgaca ctgccgttat caatatattg tttctcacgg tcaggatcga ttagccacat
     9481 aaatgttcca atagtcatcc catggctaac cacaagagca tttccgccgc cagcattttg
     9541 aatagacaaa gcaatagctt caaacccctc ccaaatgcgc ttacgaagaa cttcccatgg
     9601 ttctgcccaa ttagcggtat ctacttccac gatgctttct gccagttcag aatagggaac
     9661 atcacgtagg ttatcacgat tttcaaaggc ttttgttctt ggaaggacac ctagaaataa
     9721 ttctgaatca taagtccctt ctagactccc aaaacaccac tcacgaatac gattatcttt
     9781 agtataaggc aaaaattcgt tttctgattc tcttaaaata atttctattg tctgcatggt
     9841 tcgacctgaa tcacttgaaa acgcagcctt aaatggtata gttgcatctt ttaaacctaa
     9901 gccaagttct cgaatacctt cttctccttt ttttgtaaga ggtgtgtcac tccaaccctg
     9961 agcgcgacca atggtattaa acatcgtttt tccatgtctg gcaatataca accttgtttt
    10021 tgtcatttca acctcctaaa ctgtcttatt aattattata gcatttaaag aaaacacttg
    10081 caaaacactt cctgatagct atttgagatc aaatagacca ctgacttatt gtctatttta
    10141 ttaaaaataa tgctattgct acaattcaaa tcatcacata aatttccaaa cgattacatt
    10201 tgtgcgtttc gctcaaatat atataacaac agacccacac tacaactaaa ctgacattac
    10261 aaaatgatgt caaaacagta acaaaatcta actatttgtc aagaaaatga tattaaattt
    10321 tcacacaact atcttataat gaggatgcaa ccaataaaaa gataagacat tttatgttgc
    10381 aaaaaaggct gcttggaaac ttccatgcag ccttttttta ataccttttt ccaaaaatag
    10441 ctgaaacaag tacaattaca attgccgctc ccaaaatgga cgggaaaata gccatacctg
    10501 ccaactgaat tccccaatcc ccaaaaagcc tttgcccaac agctgaccct atgagacctg
    10561 cgataatatt agtaaaacaa cccatggact ggcttttttc agtcatttta cctgctatca
    10621 tgccgataaa ggcacctaca attaaagacc atatcatcgt taacacctcc tttcttgtaa
    10681 aatcctttta gaagaactgc tatccattaa atgttcttaa ctttagttga ttcctttacc
    10741 ttcttcttta gtaaatgttt ccgtgcctga gtaaaataga aaaaacaatt ccttctatta
    10801 aggtagatag accgagaacg actagaagtc ttcacacact aaataactta acaaacttta
    10861 tcacacagct aatcgctaat aatagtgtag acaaacgtga tagttcatta aatacacgtc
    10921 agaaactcct gaaggttcta aagcaatacc tgtcagtctt attttagcat agatgatggt
    10981 ctttttatag acggtcacta acatgacata caaaaaggca gcctgtgaaa gaccgaccgc
    11041 ctttttttca gagacaaaat actttaactt attgctatca gcaactatca atctcgcaat
    11101 ttttgcaatt cttgtgccaa taattgttga gcgacttgtg gattagcttg acctttggtt
    11161 gccttcatca agaagcctgt gaaagcctta tcagcattac gtttgcctga tttgaaatca
    11221 gccactgcag cttcattatc agcaaagact tggtgaataa ttggtaccaa aacagcggga
    11281 tcagaaatct gaacaagtcc tgccttttcg acgtaggcgc gagctgagcc accgttttta
    11341 gccaaatgca caaagacctt cttagccatt ttagaagaga ttgtaccatc agcaataata
    11401 gcaatcattt ccactaagtt gtcaggcgtc aatctaattt cttcaatggt tttgccttct
    11461 gcatttaaga attgggcaac ttccccttgc aaccagttag agacttgctt ggcatcgcca
    11521 ccaagactta ctgcagtttc aaagaaatct gacaaggctt tggtagctgt tagttggcta
    11581 gcatcataag ctgagaggcc aagttcctct tcgtatttag cacgacgctg agctgggaat
    11641 tgaggtaatt gagcacgcat ctcgtcaatc caagcatcat caatctcata taatggcagg
    11701 tctggttctg ggaaataacg atagtccgct gccccttctt tgacccgcat caaaatagtc
    11761 cccttattcg cttcgtcata gcggcgcgtt tcttggcgta tcacaccacc tgaacgcaag
    11821 agtttggctt gacgttccac ctcaaattca agccctttac ggacatttga gaaggagttc
    11881 aagtttttca attcggtctt tgttccaaat tgttcttgcc cgtaaggacg aagagaaata
    11941 ttggcatcca cacgcatgga gccttcttcc atcttgacat cagaaatacc cgtatactgg
    12001 atgatttctt tcagagctgt caaataagca taggcttctt caggagaacg catgtcggct
    12061 tctgacacaa tctcaatcaa aggaactcct tggcggttaa ggtccacata agaataacca
    12121 tctgtgccat gagtgttttt accagcatct tcttccaagt gtgcgcgttc aatacgaatt
    12181 ttcttagtcg agccgtcttc caatttaatc tcaatccatc cgttgtagcc aatcggctcg
    12241 tcaaactgtg aaatttggta agctttagga ttgtctgggt agaaataatt tttgcggtcg
    12301 aaatgcatct ctttatggat gtccatattg agggcaagcg cagcttttat gcccgcatca
    12361 atcacccctt tgttcatgac tggtaagacc cctgggaaag accaatcaat cacattcgtg
    12421 ttggcatttg ggtcctcacc aaagtgagct gacgaaggtg agaaaatttt ggaattggta
    12481 tttaattcca catggacttc aagcccaata atggtttcaa aattcattac ttgtcacctc
    12541 caaaaataat cggttgttgc ttgtgataat ctgtcaccgc ttcaaaggct gctgccgcct
    12601 gatagatggt ttcttcagcg tatttcggac caattaattg aagccctact ggcaagccat
    12661 cgacgaatcc tgctggaatg gaaattcctg gcagaccagc caagttaact ggaatggtca
    12721 ataagtcggc caaatacatg gcaacaggat cgtggttgag tgtgtctaag ccaaaagcaa
    12781 ccgtcggtgt tgttgggcct aaaataagat cataatccgc aaagaccttg tcaaaatctt
    12841 caataatcaa ggtacgcact tgaccggcct ttttgaaata agcgtcataa taacctgatg
    12901 acaaactaaa ggtcccaagc atgatacgac gtttaacctc atcaccaaag ccttggctac
    12961 gagtgttaac atagatttca tccaagtttt tagcatcgtc agcacggaaa ccatagcgaa
    13021 tgccatcaaa acgttgtaag tttgaggacg cttctgacga agcaataatg taatagacag
    13081 ccacaccata tttgctatgt ggaagactaa cttcctcaac agtagctcct aaggcttcaa
    13141 attgtttagc tgccgcaaga accgtttcct taatctcagg atcaatccct tcgccaagat
    13201 actctttagg aagggcaatc ttcatgcctt taatgtcacg accaatctta gatgtgtagt
    13261 ctgcaatgcg gacaggcgct gaagtggcat ccttgacatc gctacttgca atcacattta
    13321 ataattgggc gttttcttta actgtcggag caaaaggtcc aatctgatca agtgaacttc
    13381 caaaagcaat gagaccatag cgagaaacag tgccataggt tggtttcaaa ccgacaacac
    13441 cattaaatgc agctggctgc cgaatagaac caccagtgtc tgaaccaaga gataaacgga
    13501 cttgtcctga agcgactgct gttgccgaac cacctgatga gccaccaggt acttttgtgt
    13561 ggtcccaagc atttttcgtt ttcttgaaat aagaggtttc tgttgaacca cccatggcaa
    13621 attcgtccat gttggttttt ccaatgacaa tcatgtcttt agcgtaggca tttgctactg
    13681 atgtggcatc aaaaatcggc tcgtagttat acaacatttt tgaagcggct gtggttagga
    13741 tgcccttggt tgagatatta tccttaacag ctagtggaat acccgacatg aggttatcag
    13801 catcaatacc tttggcatca attgccgcag cttgtttaag agcagcttct tctgaaacag
    13861 tgataaacga cccgacagcc tcctcacgag acttgatgtc ttcaagtgtt ttttgagtca
    13921 attcggttgc ggaaatctcc ttagctacaa ggaggtcatg caactcttca atggttttat
    13981 gattaaaaga cattaggcat ctcctccgtc atccaaaata gcaggtacct tgataaagtg
    14041 attttctttt tcaggaacat ttttaaagag
//
LOCUS       DAUVUY010000051        13939 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.051, whole genome shotgun sequence.
ACCESSION   DAUVUY010000051 DAUVUY010000000
VERSION     DAUVUY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13939
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.051"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(1..1096)
                     /gene="hasA"
                     /locus_tag="VOX58_001238"
                     /pseudo
     CDS             complement(1..1096)
                     /gene="hasA"
                     /locus_tag="VOX58_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
     gene            1536..2780
                     /locus_tag="VOX58_001239"
     CDS             1536..2780
                     /locus_tag="VOX58_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HES5878467.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSMLLNNALLSEDYNKNIIERIYTSSYIDSSYSIKNWIKGVNEVN
                     KADIIKVANLLKLQTVYFLEGI"
     gene            2782..4071
                     /locus_tag="VOX58_001240"
     CDS             2782..4071
                     /locus_tag="VOX58_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HES5878468.1"
                     /translation="MTKLVKINYPNIDEDLYYVKLENGLTVYFIKKIGFLEKTAMLTV
                     GFGSLDNKLTVDDESRDAPAGIAHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQ
                     TSFFFSTASKFQENLELLQYFVLSANITDESVSREKKIIGQEIDMYQDDADYRAYSGI
                     LQNLFPKTSLANDIAGSKASIQKITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLA
                     IQRFQTTLSYPDRKRVTVDPLHYYPVIKSSSVDMDVTIAKLVVGFRGYLTLTQHSLLT
                     YRIALKLFLSMLIGWTSKIYHTLYEDGKIDDSFDVDVEIHHHFQFVLISLDTPEPIAM
                     SNYIRQKLATIKISKEFTNEHLNLLKKEMYGDFIQSLDSIEHLTHQFSLYLSDSDKET
                     YFDIPKIIERLTLKDVVTIGKAFFEKADASDFTVFPK"
     gene            4121..5146
                     /locus_tag="VOX58_001241"
     CDS             4121..5146
                     /locus_tag="VOX58_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5878469.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIAILGTVAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            5160..5702
                     /gene="pgsA"
                     /locus_tag="VOX58_001242"
     CDS             5160..5702
                     /gene="pgsA"
                     /locus_tag="VOX58_001242"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HES5878470.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            5704..6543
                     /locus_tag="VOX58_001243"
     CDS             5704..6543
                     /locus_tag="VOX58_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HES5878471.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            6519..7361
                     /locus_tag="VOX58_001244"
     CDS             6519..7361
                     /locus_tag="VOX58_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HES5878472.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELKEV
                     LKHG"
     gene            7354..8154
                     /locus_tag="VOX58_001245"
     CDS             7354..8154
                     /locus_tag="VOX58_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HES5878473.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
     gene            8526..9140
                     /locus_tag="VOX58_001246"
     CDS             8526..9140
                     /locus_tag="VOX58_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HES5878474.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKDKSKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
     gene            complement(9554..10426)
                     /gene="sdaAA"
                     /locus_tag="VOX58_001247"
     CDS             complement(9554..10426)
                     /gene="sdaAA"
                     /locus_tag="VOX58_001247"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HES5878475.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKEQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIDSQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(10438..11109)
                     /gene="sdaAB"
                     /locus_tag="VOX58_001248"
     CDS             complement(10438..11109)
                     /gene="sdaAB"
                     /locus_tag="VOX58_001248"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HES5878476.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGISIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
     gene            11407..12528
                     /gene="mnmA"
                     /locus_tag="VOX58_001249"
     CDS             11407..12528
                     /gene="mnmA"
                     /locus_tag="VOX58_001249"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HES5878477.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVAVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
     gene            13304..13621
                     /locus_tag="VOX58_001250"
                     /pseudo
     CDS             13304..13621
                     /locus_tag="VOX58_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
     gene            13745..>13939
                     /locus_tag="VOX58_001251"
     CDS             13745..>13939
                     /locus_tag="VOX58_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HES5878478.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREI"
BASE COUNT         4642 a         2225 c         2524 g         4548 t
ORIGIN      
        1 taaagcaacg ataaagatga tggataaaaa ggcaaaaagt ttaataaggt ctaattgaat
       61 agcttgatta aacaaaagat tcccaattgc gacaatcaac atcataaaca taacgacttc
      121 gaaaatagtc cataaggcaa cgatgggatt agaaagaatt tttttaacag aaataataga
      181 ttctctaaaa aaagatttat tccatcgatt ttgttgcttt aaataacttt ttaattggaa
      241 aggtacatca gtatcacatc tagctgttga ttggtagaca gtgcgtccta aatcaatagc
      301 ataatttgtt aaacatcgat catccccaat gctaacaggt aaacctagga atgtttgatt
      361 tttatagcgc tctaagttag gaataatcac ttcacgtcga taaatactca atggtcctga
      421 gcaaactaaa atattacctg ttaatgattg agcagcacgc tccaccccaa aggcattatc
      481 gtaacggata tctgtaagtc gcgttaatag attagtttgt ctgtttctag cattcaaatg
      541 tcctgttgca gcataaactg tcttatcatt gaagcttttt aggagttctt ctaaggcatt
      601 tggatagata taagtatctg aatctacggt taaaaaaacg tcagcgtcag atctttcaaa
      661 tgcccacgct tgagcatggc gttttccttt attgacaagg gaacggtgaa cgataacgtt
      721 tcgacaaata tccacttctc tatttacata ctcttcaatt aattgtattg catctgtgtt
      781 tgaactccca tcatcaacaa tataaatttc tgataacgga taggtctgtg ctaacacact
      841 tttaagagtt tctaataatg actcggcatc ttcattatta agaaggaatt acggcagcaa
      901 ctttatagtc atgtggattt cctttaaatg gctcataaag gaaagataat ccaagtttaa
      961 taactagata ggttattaat attactccat aaattcctac agttgatgtt ccaaatagat
     1021 acatatttag ataaatcaag atagatatca aaaaaataaa ggataaaaca attaaagttt
     1081 ttttaaaaat aggcacaatt acacctcttt cttttttaat ttccttgaaa gacagggacc
     1141 tcgatattct aatataaata agggataatg tcaactagtc atagaaatat ttttaataaa
     1201 aatgtattta aattaaaaaa tttcccccaa aaaaagaaaa atcaggataa tttatttaat
     1261 ataaattaaa taaattatct caaaataatt gaattgttat aaattatttt agcaaacttt
     1321 tcaaatagtt ttatctaatt ttgtgaaaaa aatagatata ttttgatttt taaaaaatat
     1381 caagaaatat gataactggt aaaggatata ttgtagaaaa ctatatttta ctaacactag
     1441 aatcccgaca attaagtcaa aaaaagttat cactcaccta acctgaataa aaatggtata
     1501 atcgtaagga tcacactatt tattaaggaa acattatgaa aattgttcag ggagtacaac
     1561 ttcatctgat taagaccaaa cagtttaaaa ccaatcatat tacctttcgt ttttcaggtg
     1621 atttgaacca aaaaacagta gcaaaaagag tcttagtggc acagatgtta gcaacagcta
     1681 atgagtgtta tccaactgtt aggcagttta gagaaaaatt agcacgactt tatggagcta
     1741 gcttgtcaac taacgtgtta acaaaagggt tagttcatat tgtggatatc gatattactt
     1801 ttattcaaga tagatatgct tgcaatgggg aaaaaatact agatgaaatg attcagtttt
     1861 taaaagatat cttgttctca cccctattat ccatcgccca gtatcagcct aaagtttttg
     1921 agactgaaaa aaataatttg attaactaca tcgagtcaga tagagaggat tctttttact
     1981 acagctctct taaggtaaaa gaattgtttt attgtaataa gaatctacaa atgtcagaat
     2041 atggtagccc agaactaatt gcaaaagaga ctgcctatac tagctatcaa gaatttcata
     2101 aaatgctcaa tgaagatcag attgatattt tcattttagg agattttgat gattatcgcg
     2161 ttgttcagct aattcatcaa tttccacttg ataaccgaaa taaaaaccta aatttctttc
     2221 atttacaaaa ttctgtcaat attattaaag aatctattga aaaaagagca gttcatcaat
     2281 ctattttaca attagcttat cacttccctt cagtttttgg acaacgtgac tattatgctt
     2341 tagttctttt aaatgggttg ttaggctctt ttgcacattc ccgtctcttt ataaaaatta
     2401 gagaagaaga aggacttgct tatagtatag ggtgtcgttt tgatagttat acaggtttat
     2461 ttgaaattta tactggaatt gacagtcaac atcgcactaa aacgttacaa ctaatcatcc
     2521 aagaattaaa tgccattaaa atgggacgat tctcagaaca attgattaaa aagaccagat
     2581 cgatgttgtt gaataacgcc ttattatcag aagattataa taaaaatata attgaaagaa
     2641 tatacacatc ttcttacatt gattcttctt attctattaa aaattggatt aaaggggtta
     2701 atgaagttaa taaagcagat atcattaaag tagctaatct tttaaagctt caaactgtct
     2761 attttttaga aggaatataa aatgacaaaa ttagtaaaga taaactatcc taatattgac
     2821 gaagaccttt actatgtaaa attagaaaat ggtttaactg tttattttat aaaaaaaata
     2881 gggtttttag aaaaaacagc tatgttgaca gtaggtttcg gttctcttga caataagttg
     2941 acagtcgatg acgaatcacg agatgctcca gcaggtattg cacacttttt agaacataaa
     3001 ctctttgaag atgaatcagg tggagatatt tcattaaagt ttacacagct aggagcagag
     3061 acaaatgcct ttacaacttt caaccaaacg agtttcttct tttcaacagc tagtaaattt
     3121 caggaaaatt tggaactcct tcaatatttt gttctttctg ctaatataac agatgaatct
     3181 gttagtagag aaaaaaaaat aatcgggcaa gaaattgata tgtatcaaga tgatgctgac
     3241 taccgtgcat atagtggaat ccttcagaac ttatttccaa aaacaagttt agctaatgat
     3301 attgctggct caaaagcatc cattcaaaag atcacgaaaa tattattgga aacgcatcat
     3361 acttattttt accaacctac taatatgagt ctattcattg tcggtgatat tgatattgac
     3421 gaaacttttt tagctattca aaggtttcaa accacattat cctatccaga tagaaaaagg
     3481 gtaactgttg atcctttaca ctactatcct gtcattaaat catcttctgt tgacatggat
     3541 gttactattg ccaagctggt tgttggtttt cgagggtatc tgaccttaac tcagcattca
     3601 ctattaacgt atcgtattgc cttgaagcta tttttatcaa tgcttatagg ttggacttct
     3661 aaaatctatc atacgctata tgaagatggt aaaatagacg attcatttga tgtggatgtt
     3721 gaaattcatc atcattttca atttgtatta atatcacttg atacaccaga gcctatcgca
     3781 atgtctaact atatccgtca aaaattagcc actataaaga tatcaaaaga gtttacaaat
     3841 gagcacttaa atctattaaa aaaagaaatg tatggtgact ttattcagag tttagactct
     3901 atagaacatt taacccatca gttcagtcta tacctgtctg attctgataa agaaacatat
     3961 tttgatattc caaaaattat agaaagactc actctaaaag atgttgttac aattggaaaa
     4021 gcattttttg aaaaagctga tgcttcagac ttcacagtct tcccaaaata gatgaaaatc
     4081 tgttataata ggattactat attatttttg aggtacctgt atgagtgaaa aaagtcttgg
     4141 tgaagtgtta agagaatcgc gtgttggaaa aaatatcaca ctagatgata tagaattgaa
     4201 gacagggatt tcttcccatt acttgctggc aatggaattg gatcagttta aaattattcc
     4261 ggaagaaaaa tttgatcaat ttttaaaaga atatgctgac attgttgagt tagatttcaa
     4321 tactttaaaa agacgatacc gttatcaagt taattccaaa aaagacagtg atataaaatc
     4381 tgtaactgag attgttgaag agaagttaag taagaaacga ttacaagaga agttgttatc
     4441 acatcagagc atggggtatg gtcaaccgtt agaagtttct aaaaacaaag aaaccttagt
     4501 tatccctgta aagactgttg gtttagatag caaatcgcca ctaaaaacga tatcgcctgt
     4561 tagccaattt aaatcagata acaggttaat tcctaaaaaa aatgtgtcac gtatgaggcg
     4621 aaatagcact gacagtcgtt ctaaaacgtc actatttccc atagctatat taggaacggt
     4681 tgcttttgca attatcagtg ttatcatttt aggtgtttgg caacaattcg aaaagagtca
     4741 aagagctaaa gaagctgaag ttgctttgct taaagcctct gagcagtcta ctaacggttt
     4801 aaaaaataat tcaaatgatc gcaaaactca agtcacaact gaggggtcgg gtaattattt
     4861 aatagcgact gttactaaat ctaaagagac cgttgatatc agtgtgtctt taactgaggc
     4921 acaaagtagt tggatttctc taacaaattc agaaattggt gaaggtggta taacattgac
     4981 tcaagactcg ccaacttata cagcgacttt acctgcagaa ataacagaat cattgttgac
     5041 acttggtgtg acaaaaggtg tatctgtaac aattgatgga caacctgttg atctatcacc
     5101 tttaacaagt actgacttaa gttatatcac gtttaagatc caataattaa ggtagactaa
     5161 tgataaaaaa agaaaatatt cctaatctat taactcttgt tcgaatcgca atgattcctt
     5221 tctttctttt tattacttcc tcatctaata aggtggggtg gcatattttt gcagctgtga
     5281 tttttgcaat tgccagtttt accgattatc tagatgggta tcttgcgcgt aagtggcatg
     5341 tagccagtaa ttttggaaag tttgccgatc ctctagcaga taagatgctt gtcatgagtg
     5401 cctttatcat gttagttgga cttggcttag ttcctgcgtg ggtgtcagct gttattattt
     5461 gccgagaatt ggcagtaact ggtcttcgat tactacttgt tgaaaccgga ggaaaggtcc
     5521 ttgcagctgc tatgccggga aaaatcaaaa cagcgacaca aatgttgtct attattttat
     5581 tactttgcca ttggatattc ctaggaaacg tcttactcta tattgctctt tttttcacta
     5641 tttattctgg atatgattat tttaaaggag caagctttct ttttaaggat acgtttaaat
     5701 aacatgtcag ctattattga acttaaaaaa gttacattta attaccataa agaccaagaa
     5761 aaaccaacat tagatggcgt atcgtttcat gtgaaacaag gtgagtggct gtctatcatt
     5821 ggccataacg gatctggtaa atcaacgact attcgattaa ttgatggatt attggaacca
     5881 gaatcagggt ctattattgt tgatggagat ctactaacca taactaatgt ttgggaaatt
     5941 cgtcataaaa ttggtatggt ctttcaaaac cctgataatc agtttgttgg agcaactgtt
     6001 gaagatgatg ttgcttttgg gcttgaaaat aaaggtattg cacatgaaga tataaaagag
     6061 agggttaatc atgctttaga gttagtcggc atgcagaact ttaaagaaaa agaaccagcc
     6121 cgtttatctg gtggccaaaa acagcgcgta gctattgcag gtgcagttgc tatgaagcct
     6181 aaaattatta ttttagatga agctactagt atgcttgacc ctaaaggacg attagagtta
     6241 atcaaaacta taaaaaacat ccgtgacgac taccagctga ctgttatttc cattactcat
     6301 gacttagatg aagttgctct tagtgataga gttttagtga tgaaagatgg tcaagtggaa
     6361 tcaacatcaa caccagaaca attatttgca agaggggatg aactactcca attaggtctt
     6421 gatatccctt ttacaacatc tgttgtacag atgcttcaag aagaaggtta tcctgttgac
     6481 tatggatatc tcacagaaaa ggaattagaa aatcagttat gtcaattaat ctccaaaatg
     6541 taagttatac ttatcaagca gggactcctt ttgaagggcg tgcccttttt aacattaatt
     6601 tagatatttt agatggttct tatacggctt ttatcggaca tactggatcc gggaaatcga
     6661 ccattatgca attgctaaat ggtttacacg ttccgacaac gggaattgtc agcgtagata
     6721 aacaagatat aacgaatcac tctaaaaata aagagattaa atctattcga aaacatgtag
     6781 gattagtttt tcaatttcca gaaagtcaac tatttgaaga gacagttttg aaagatgttg
     6841 cttttggccc tcaaaatttt ggggtctctc cggaggaagc agaagcttta gcgcgtgaaa
     6901 aactagctct tgttgggatt tcagaaaatc tttttgaaaa gaatccattt gaactctcag
     6961 ggggtcaaat gcgtcgtgtt gctattgcgg gtatccttgc tatgcagcca aaggttttag
     7021 ttttagatga accgactgct ggtcttgatc ctaaagggcg taaagaatta atgactattt
     7081 ttaaaaagtt acaccaatct ggcatgacta ttgttttagt aacccatctg atggatgatg
     7141 tggccaatta cgcagatttt gtatatgttc ttgacaaagg taaaattatt ttatcaggaa
     7201 aacccaaaac aatttttcaa caagtcagtt tgcttgagaa aaaacagtta ggagttccta
     7261 aggtgactaa acttgctcaa aggcttgttg atagaggaat acctatctct tctttgccta
     7321 ttaccttaga ggagctaaaa gaggtactaa aacatggata agctgattct tggtcgttat
     7381 attcccggag attctctcat tcatcgtctt gacccaagaa gtaaattatt agcgatgatt
     7441 atttatattg tcattatctt ttgggctaac aatgttgtga caaatttgtt gatgttgact
     7501 tttactttag cagttgtttt tttgtcaaaa atcaagttgt ctttcttttt aaatggggtt
     7561 aaaccaatga ttggtattat cctcttcacg actttatttc agatgttttt ctcacaagga
     7621 ggaaaagtaa tttttagttg gtggttcatt agcataactg atttaggatt gagccaagct
     7681 attttaattt ttatgcgttt cgttctaatt attttctttt caacgctgtt aacgttgacg
     7741 acaacacctt taagcctatc tgatgcagtt gaatcattat taaaaccttt aacaagattt
     7801 aaagttccag ctcacgagat cggactaatg ctttctttaa gtttacgttt tgtgcctact
     7861 ttaatggatg ataccacgcg tattatgaat gcacaaaggg cccgtggagt tgattttgga
     7921 gagggaaatt taattcaaaa agttaaatct attattccta ttttaatccc tcttttcgct
     7981 tcaagcttta aaagagccga cgcattagct attgctatgg aagctagagg ttatcaggga
     8041 ggagaagggc gaactaaata tcgtcaatta gactggcagc taaaagatag tttagctatt
     8101 ggaatagttt ctttattggg tttgctactt tttttcttga aaacctcttt gtaatatttt
     8161 ttagcacaaa acttttcttt attagtaaga agtgaattgt acctcaaaag ttagatacaa
     8221 catctaatga ttgaggtact tttcttcatt tgaggaattg aaaaccgcta tctcaactat
     8281 attaatcctc ccaataataa caaatcgaac gaaatctaaa aggactgagt cctgttcaat
     8341 acagaactaa attattagtt taatgatatt atcccgcttt ttaatgttgc taagaagaag
     8401 agttttatga ttttttacaa cttcttataa tatcaatgta gttgtaatat ttttaatata
     8461 tcttcaataa cgaggtaata attaaaagat attatggata acggagaaaa gtgaggtatt
     8521 attgaatgtt taagaaagaa aatttaaaac aacgttattt taattttgga ttagtagcgt
     8581 tagctctaac aatattagcc atcatttttg ccttctcaag taaaaatgct gatactaagt
     8641 cttatgctaa gaagtcagaa agtaaaatgg taacaatcga caaggctcca aaaaataatc
     8701 atgctattac taaagaagaa agcaaagata aatcaaagag cattgcttcg gagcctattc
     8761 ccacagtaga aaactctgta gctccgacag taacagagga agcaccggtt gttcagcaag
     8821 aagtgactca aactgttcag caggtatctt cagtagccta taatccaaac aatgtggtac
     8881 tttccaatgg aaatactgct ggtattgtag gaagtcaagc ggcggcacag atggcagcag
     8941 caacaggtgt tccacaatca acttgggaac atataattgc gcgtgaatct aatggaaatc
     9001 ctaacgcagc taatgcttct ggggcatcag ggttgttcca gacaatgcca ggttggggtt
     9061 ctacagcaac ggttgaagat caagtcaatg cagccttgaa agcctatagt gcacaaggtt
     9121 tatcagcttg gggttactaa ataaacatag ctggaagaaa taaaacctat tgttagtatt
     9181 tactaacaat aggtttttgg tatttaattg tctttatgat ttgtagtgat gaattagtct
     9241 caaaacaaaa cattgcctta tcagggaata tagctcgtat tatttaggaa aactgaccag
     9301 cacttaagtg cctctgatat ttaaggcagt tatactgata acaagcagga aaaaaactct
     9361 taaatgttta taagatcatt agcaattttc agtattttac actactacac ttttgagttt
     9421 taacaattta ctagaagttg tttctaaggg gaaacaacat agcttgatag ctatctatta
     9481 gtcattttag tgaatatata gcagaaaaat aaaacgcttt caaatatttc taagaatttt
     9541 tctaactatc tatttattcg ccaaatattt ctacgctgta acgtcgtcca gttggagtag
     9601 cggctaaacc accttctgct gtttcacgga aagcagttgg catggcagag ccgacttgat
     9661 acatagcatc aataacttcg tcaacaggaa tttgagaatc aatatcagct aaagccatat
     9721 cagctgcgac aagggcgaaa cttgctccta gagcattgcg tttgacacaa ggcacttcta
     9781 ctaggccggc aacaggatca cagacaagtc ctaataaatt tttgataaca aacgcaatag
     9841 cttgactagc ttgatgagat gttccaccag ctgctttaac aagagcagct gcactcatag
     9901 cagctgcaga accaacttct gcttggcatc caccttctgc accagaaata gaggcgttat
     9961 ttccaataac aagaccaaaa gctcctgcag taaataaaaa ctctaattgt tctttttctg
    10021 ataagtcaag tttttcaata gctgttgcga gcactgctgg taagcatcct gcacttcctg
    10081 cagtgggtgt tgcacagaca agtcccattt tagcatttaa ttcgtttaca gctatagcat
    10141 ttctaacagc attaagaata gtagtatcag aaaggctgtt ccctttttta atgtagttat
    10201 ccattttgac cgcatcgccc cctgtcaaac cactgataga tttggttgag gttagtcctt
    10261 ctgttacagc tgctttcata acttgtaaat tgcgtgacat gattttgata atatcttctc
    10321 gatttcttcc agacatctcc acttccgtgg caatcatgag ttcagcaata tttccattaa
    10381 attgttggtc agcttgtttg acaagttctt caatagtata aaacatagct ttcctttcta
    10441 gtcaaagaag ttgacattat aaatatgagg aatttttgca atttggttgg ctgcttcttg
    10501 gcactctcta gaatcgactt ctataatcat agtagctttt tcaccagcgg attcacgtgt
    10561 aacattcatt gtggcgatat taatattatt ggaagatagg atatcagtta cctttgcgat
    10621 cattccggga atatctttgt gtacagtaac tatagtaggg gtattcatgg ataacgagac
    10681 agaaaaacca tttagttctg ttacttgaat gtttcctcct ccaattgaaa taccagtaac
    10741 actaagtgtt ttgtcagctt ttttgacact gattttaaca gtattgggat ggggagcatt
    10801 actatctttt aagatatccc aatagatttt tatccctttt tgatgagcaa tttctagaga
    10861 atttttaata tcaggattat cagtatccat acccataatt cctgcaacaa gagccttatc
    10921 agtaccatgt cctctgtagg ttttagcaaa tgaattataa aggtgaaacg tcacttcatc
    10981 aggaatatct ccaaagatgg aatggactac cttaccaatt ctgacggcac cagctgtgtg
    11041 actacttgat ggaccaatca ttactggccc aatgatgtca aaaactgatt gaaatttttg
    11101 tgtattcata ggcaactccg ttttttattt ctatgtttat tataacaaaa aaatatcaaa
    11161 aaacagcaat ataattataa aaaattattt ttataataaa aaattattga gcaataaaat
    11221 gattggcatt ccacgcagta attgttataa taaggtttgc gctgatggcg cttcaaaatc
    11281 gaccttcaaa tcgacttttg aaaacgacga ccttcaatcg tacgttaact gataagaaaa
    11341 agaatcataa tattcataat atgatgatta tctttttcta tcttattatt gatgggagaa
    11401 ttttttatga cagataactc aaagatacgt gttgtcgttg gtatgagtgg tggcgttgat
    11461 tcatctgtaa cagctctact tttaaaggag caaggttatg atgttattgg tgtcttcatg
    11521 aaaaactggg acgatacaga tgaatttggt gtttgcacag ctactgaaga ttacaaagat
    11581 gtagcagccg ttgctgatca aatcggtatt ccttactact cagtcaattt tgaaaaagaa
    11641 tattgggaca gagtatttga atatttcttg gccgaatacc gagcaggtcg tactccaaat
    11701 cccgatgtca tgtgtaataa ggaaataaaa tttaaagctt tccttgacta tgccatgact
    11761 ttaggtgcag attatgtagc aacgggacac tatgctcaag tcaagcgtga tgaaaatggt
    11821 acggttcaca tgttacgtgg agctgataat ggcaaagacc aaacctactt tttaagtcaa
    11881 ctatcacagg aacaactaca aaagacttta ttcccccttg gtcatttgca aaagtcagaa
    11941 gttagagaaa tcgctgagcg agcaggactt gcaacggcta aaaagaaaga ttcaacaggt
    12001 atttgtttta ttggtgaaaa gaatttcaaa cagtttttga gccagtactt accggcacaa
    12061 aaaggtcgca tgatgactat tgatggtcgc gatatgggag aacatgccgg tcttatgtat
    12121 tatactattg gtcagcgtgg tggtctgggc attggtggtc agcatggtgg ggataatcaa
    12181 ccttggtttg ttgtcggtaa agatttgtcg caaaatattt tatacgttgg tcaaggtttc
    12241 taccatgaag ccctaatgtc aaatagtctt gacgcctcag tcatccattt tacacgtgag
    12301 atgccagaag agttcacatt tgaatgtact gctaaatttc gctatcgtca acccgattca
    12361 cacgtggcgg tgcatgttcg aggagataag gcagaagttg tttttgcaga gccacagcgc
    12421 gcgattactc ctggtcaagc tgttgttttc tatgacggaa aagaatgcct tggtggtggt
    12481 atgattgaca tggcttataa aaatgggcag ccttgtcagt acatttaatg aatcttttat
    12541 taagataaaa agaaagggac attcaacaat tccttataca tcatatgata ttatttataa
    12601 aaaccaatca tgcgttaatg gattaattta tgatatctgt atattttaaa tataatcaca
    12661 ttatttttaa aaatagttcg taaaagttga gtcattactt tattagttac tgtagaaatc
    12721 atacgatcat gagactcact ttctctagta ttacaacaga tattttgatg attaggtatt
    12781 tcgtaaaaca gattatttca tgattattag tacctattat ctaatactaa atgtgtaaaa
    12841 aattattgga aatttctagt tatactttta tggttttgtt atcggcgttc ttacttgtag
    12901 acttagaaac aacggtatct gtattttact actttagaag atatcaaaaa atcgtatgat
    12961 aatactgatg acatttttat ttctcgcctc gctagattag acatagtgat acttttgtaa
    13021 tcattttaaa aacattacaa tagattgaca gagatgtaaa aacattgaca ttactgtaaa
    13081 ggtctgtgat gaaatcaata ttgacaatca ctaatcattt ttttaaaata gataataaca
    13141 atcggaatga tggtcaaagc tcatgaaagt tgtaggtttt tttgtcctgc aatcttctag
    13201 ggttttggcc ctttttatgg tttagctcta gaattgagat ggctaatagg actgggattt
    13261 agaatcacta gaggtcagat aactttagtt tacgagctag tgcttgattg ttagaaatgg
    13321 taagaatttt ttaactagag atgcagctga ctaatattac accattggtg gtacaagttt
    13381 ggtaggtgaa aaaacgcatg aaactgtttt acgtgaaacc ttagaggaag ttggtattcg
    13441 aactaaggtt aatcaactgg cttttgtggt tgagaatcat tttgatatcg atgatgtttt
    13501 ctggcataat attgaatttc attatttggt aagcccctta gaagacccta aactagaaat
    13561 gatagagaat gcaagcgaca ggtttgtgaa tggattccaa tcaatcaact ggatcaaatg
    13621 aatcttgttc ctgagttttt acaaaccgag ttggctaaat ggccaggaca tattgtccgc
    13681 attgaaggct aaactaaagc gtatgatttt aaatgaatag actgaaataa aggattttaa
    13741 aaaaatgaca catgaattta cagaaagcta tgacgtcatt gtcataggtg caggtcatgc
    13801 tggtgttgaa gccagtttgg ccactagtcg tatgggttgt aaaactttat tggcaacaat
    13861 caaccttgat atgttggctt ttatgccgtg taatccttct attggaggat ctgccaaagg
    13921 gatagtggtt agagagatt
//
LOCUS       DAUVUY010000052        13869 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.052, whole genome shotgun sequence.
ACCESSION   DAUVUY010000052 DAUVUY010000000
VERSION     DAUVUY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.052"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..207
                     /locus_tag="VOX58_001252"
     CDS             <1..207
                     /locus_tag="VOX58_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HES5878479.1"
                     /translation="IMPEIEAELTDMTFICVNRDQFIEVAQKWNIFGIPSFVVIEKGQ
                     EVGRLVNKMRKTKTEIMHFLAAYQ"
     gene            225..851
                     /locus_tag="VOX58_001253"
     CDS             225..851
                     /locus_tag="VOX58_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HES5878480.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEVSSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPTVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELVLPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            1003..1398
                     /locus_tag="VOX58_001254"
     CDS             1003..1398
                     /locus_tag="VOX58_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HES5878481.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISIVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(1659..2300)
                     /locus_tag="VOX58_001255"
     CDS             complement(1659..2300)
                     /locus_tag="VOX58_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HES5878482.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(2320..3297)
                     /gene="dusB"
                     /locus_tag="VOX58_001256"
     CDS             complement(2320..3297)
                     /gene="dusB"
                     /locus_tag="VOX58_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HES5878483.1"
                     /translation="MTKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFKTVR"
     gene            complement(3284..4156)
                     /gene="hslO"
                     /locus_tag="VOX58_001257"
     CDS             complement(3284..4156)
                     /gene="hslO"
                     /locus_tag="VOX58_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HES5878484.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISHLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNENDLEALINDKA"
     gene            complement(4303..5796)
                     /gene="rofA"
                     /locus_tag="VOX58_001258"
     CDS             complement(4303..5796)
                     /gene="rofA"
                     /locus_tag="VOX58_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HES5878485.1"
                     /translation="MIEKYLESSIESKCQLIILFFKTSYLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSHDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRDLNHKHFHLFCHYIEQSLRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVHHELTKGIAVAEISFDESILSIQELMYQVKEEKFQADLTKQLT"
     gene            6063..7805
                     /gene="sfbI"
                     /locus_tag="VOX58_001259"
     CDS             6063..7805
                     /gene="sfbI"
                     /locus_tag="VOX58_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein SfbI"
                     /protein_id="HES5878486.1"
                     /translation="MNNKIFLNKEAGFLAHTKRKRRFAVTLVGVFFMLLACAGAIGFG
                     QVAYAADEKTVPNFKSPDPDYPWYGYDAYTGAFLRYHDLRVNLNGSRSYQVYCFNIKK
                     NYPRPFTSSDKKWYKRLEGTAETFKVHAMAPRVGGEELTKKLRSVMYNGYPNDGNNIM
                     KGLEPSNAIEVTQYAVWYYSDKSQYEIETLWKSEAKEGKISESQVTLMREALRKLISS
                     DEDLVKQVPSNFKLSTFESSDKSYQNLLSAEFVPDDPPKPGDTSEHNPKTPELDGSPI
                     PEDPKHPDDNLEPTLPPVMLDGEEVPEVPSESLEPALPPLMPELDGEEVPEVPSESLE
                     PALPPLMPELDGEEVPEVPSESLEPALPPLMPELDGQEVPEKPSVDLPIEVPRYEFNN
                     KDQSPLAGESGETEYITEVYGNQQNPVDIDKKLPNETGFSGNMVETEDTKEPEVLMGG
                     QSESVEFTKDTQTGMSGQTTPQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSET
                     VTIVEDTRPKLVFHFDNNEPKVEENREKPTKNITPILPATGDIGNVLAFLGILILSVL
                     SIFSLLKNKQNNKV"
     gene            8093..8941
                     /locus_tag="VOX58_001260"
     CDS             8093..8941
                     /locus_tag="VOX58_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HES5878487.1"
                     /translation="MTERLKNLGILLLFLLGTAIFLYPTLSSQWNAYRDRQLLSTYHK
                     QVTQKKPSEMEEVWQKAKAYNARLGIQPVPDAFSFRDGIHDKNYESLLQIENNDIMGY
                     VEVPSIKVTLPIYHYTTDEVLTKGAGHLVGSALPVGGDGTHTVISAHRGLPSAEMFTN
                     LNLVKRGDTFYFRVLNKVLAYKVDQILTVEPDQVTSLSGVMGKDYATLVTCTPYGVNT
                     KRLLVRGHRIAYHYKKYQQAKKAMKLVDKSRMWTEVVCAAFGVVIAIILVFMYSKMTD
                     KKVIKN"
     gene            9227..11470
                     /locus_tag="VOX58_001261"
     CDS             9227..11470
                     /locus_tag="VOX58_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HES5878488.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMLLVLIGFEGKV
                     RAAHELVEVPVPIFHNPDPQSDYQWYGYEAYTGGYPKYDLFKTYYHDLRVNLHGSKSY
                     QVYCFNVHKHYPRSSQSFDRKWYKKLDGTAENFDSLAMEPRVRKEELTKKLRAVMYNA
                     YPNDANGIMKDLEPLNAIKVTQEAVWYYSDSAQINPDESFKTEAQSNGINDQQLGLMR
                     KALKELIDPNLGSKYSNKTPSGYRLNVFESHDKTFQNLLSAEYVPDTPPKPGEEPPAK
                     TEKTSVIIRKYAEGDYSKLLEGATLRLTGEDIPDFQEKVFQSNGTGEKIELSNGTYTL
                     TETSSPDGYKIAEPIKFRVVNKKVFIVQKDGSQVENPNKEVGSPYTIEAYNDFDEFGL
                     LSTQNYAKFYYGKNYDGSSQIVYCFNANLKSPPDSEDHGATINPDFTTGDIRYSHIAG
                     SDLIKYANTARDEDPQLFLKHVKKVIENGYHKKGQAIPYNGLTEAQFRAATQLAIYYF
                     TDSVDLTKDRLKDFHGFGDMNDQTLGVAKKIVEYALSDEDSKLTNLDFFVPNNSKYQS
                     LIGTEYHPDDLVDVIRMEDKKQEVIPVIHSLTVKKTVVGELGDKTKGFQFELELKDKT
                     GQPIVDALKTNNQDLVAKDGKYSFNLKHGDTIRIEGLPTGYSYTLKETEAKDYIVTVD
                     NKVSQEAQSASENVTADKEVTFENRKDLVPPTGFTTDGGTYLWLLLLVPFGLLVWFFG
                     RKGLKND"
     gene            11463..11984
                     /gene="lepB"
                     /locus_tag="VOX58_001262"
     CDS             11463..11984
                     /gene="lepB"
                     /locus_tag="VOX58_001262"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HES5878489.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYVFGVMIV
                     NTNQMSPAVSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVNFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            12006..13043
                     /locus_tag="VOX58_001263"
     CDS             12006..13043
                     /locus_tag="VOX58_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HES5878490.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKSETAGVSENAKLIVKKT
                     FDSYTDNEVLMPKADYTFKVEADSSATDKTKDGLEIKPGVTEGLTTEQTIAYDNSVKP
                     SDKSKTATFDFSTVKFPEVGVYRYTVSEIDSKVSGIKYDTKTWIVDVYVVNDGNGGFK
                     ARYIVSKEKGQNDKKPVVFENSFKTTSLKVEKQVTGNTGELKKDFNFTLTINPNDNFV
                     AGQVIKLEKGGIKADVKIGEPYKFALKNGEKVTLSKLPVGVTYSIIEDEADKDGYTTN
                     AKITDGTAAPVEYKLGNQQLADESADEIVVTNNRDTQVPTGVVGTLAPFAVLSIVAIG
                     GVIYITKRKKA"
     gene            13059..13784
                     /gene="srtB"
                     /locus_tag="VOX58_001264"
     CDS             13059..13784
                     /gene="srtB"
                     /locus_tag="VOX58_001264"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HES5878491.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNNFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
BASE COUNT         4578 a         2310 c         2880 g         4101 t
ORIGIN      
        1 attatgccag aaattgaagc agaacttaca gatatgactt ttatttgtgt taatcgtgat
       61 cagtttatag aggtagcaca aaagtggaat atttttggta ttcctagttt tgtcgtgatt
      121 gaaaaaggtc aggaagtagg gcgtctggtt aacaaaatga gaaagacaaa aactgagatt
      181 atgcattttt tagctgctta tcaataagaa aagaggaaga ataaatgatt tttgcataca
      241 ataaagaaca agttggcgat gtcttgatgg ttatcttaca agacaccaaa gatatcaaac
      301 gtcaagtaga acgaaaaggc aaagtagccc gtgtttttgc agaagaaagc ggcaaaaccc
      361 ttgcttggaa tatctttgaa gtgtcaagct tgattactat tgaaggcaat ggacagattt
      421 ttttgacaga cgaggacctt gcaagattaa atgcagagct tgccaaggaa ggattttcag
      481 aaaggcttga accgactgtg ggacctgttt ttgtggttgg tcaaattgtt gagatggtgg
      541 ctcatccaga tagcgaccat cttaatattt gtcaagtggc tattggtgaa gatcaaacgg
      601 ttcaaatcgt agcgggcgca ccaaatgctg cgcttggttt aaaaacgatt gttgccttac
      661 caggtgctat aatgccaaat ggtagtctga ttttcccagg aaaattgcgt ggtgaagaaa
      721 gttatggtat gatgtgctct cctcgtgagt tggtcttgcc aaatgcaccg caaaaacgtg
      781 gcattattga acttgatgag tcagctgtgg taggagaagc ttttgaccca gccaaacatt
      841 ggaaaggtta gaaaaaccag ctgaaataag actgtattta actaaatgac cagaaaaaaa
      901 ggattaggag cccttaatcc ttttttctta tgttttcaaa aacgtttaaa atgaaactta
      961 ttttaccaca atttttacga ataataagat aaggaggtca gtatgtataa taaagtgata
     1021 gcaatcggtc gtttggtagc taaaccagaa ttggtaaaaa ccgctacgga taagcatgta
     1081 gcacgtctct ctttagctgt taatcgaaga tttaaaaatg cttcgggaga gcgagaagcg
     1141 gattttattt caattgttgt ttggggaaag ttagcagaaa ctctggtttc ttatgctagc
     1201 aaaggtagtt tgatgtctat tgatggcgaa cttaggaccc gcaagtatga taaagatggg
     1261 caagtgcatt atgtgacaga agttctctgc caatcatttc aactgcttga aagtcgtgct
     1321 cagcgcgcta tgagagaaaa taatgttact aatgatctag ttgatttagt cttagaagaa
     1381 gatactcttc ccttttgata gaggctaatg cttctcatca gggttcagct gattactgaa
     1441 atatatgatg aggtgatatc cgagcaacaa aagtgtgact agatacttct gataaaaata
     1501 aaaaaaacac cttacgagta tgaacaaagt ttcaagtgct agatgttgga actttgttca
     1561 tcataggaat agtagggtgt tttttagtgt attacttctt tttgttgcta gtgttaaggt
     1621 ttacttgctt acaagcggtt atcaggccac tagaactctt ataataaatg gagctctttg
     1681 agtttggtgt caatcatttt taagacagtc gccaaatctt gtgggttttg aacgaaatca
     1741 agggaattgc catcaatttt catttttgga gagaccttgt agtcttcgta ccaggttggg
     1801 tattcaccgt ggacttgatg gtaatattgc tcaagactag gattgccgtc gacctgctca
     1861 aaacttcggc cacgtttttc aatgcgctca agcattttgt caaaagacac atcaatgtag
     1921 atgagaagat cagggcgttt tttaggcatg ccttctagtt cttctaacat gttggcgaga
     1981 agttcctgat agatgtcaag ttctgtctta gtgacattgc catttttgta gttaagtttt
     2041 aagaagagtt cgtcttcaaa aatagagcgg tcaagaatgt tgttatcggc ttgataagct
     2101 tctttgatgg acttaaaccg tttattcaaa aaataaattt gaagtaaaaa tgcgtacttt
     2161 ttagggtctt ggtagtagag gtctagcacg gggttattat caacagcttc ataaaaaacg
     2221 tcagttccta agtgctctcc aagtgcagca gcaagcgagc tcttgccggc cccaattgtt
     2281 ccagctagta caatcaacat gcgtctcctt ctaatatcat tagcgtactg ttttgaagat
     2341 ggcttcaacc tcagctagag tctcagcgcg tgaaacagca ccacgaactt tggcagcacc
     2401 tgctgttccg cgaagatagt gtggcgctaa gccccgaaat tcccgaacag caatggtttc
     2461 acctttgagg ttaatcaggc gtttgaggtg gtctttggcg atgtctaatt ttttggcaaa
     2521 gggaagatct ggcaattctt gaccagtttc aaagaaatga ttgatttggg taaaaagata
     2581 aggattgttc atggctgcgc ggccaatcat cacggcatct acaccgattt cttcaatcat
     2641 aaatttggcg tcttgcaccg aacgcacgtc cccatttcca ataaagggaa tcttagtgat
     2701 agccttagaa actcgggcta atgtctcatg gtcgcaggtt cctgtataca tttgttcacg
     2761 ggtacgaccg tgcatggcaa gagcagatac acctgccgac tcagcggcaa gggcattctc
     2821 aacggcaagt gaactatccg cccaacctgt acgcattttg acagtcagtg gaatatctaa
     2881 aacagacgtg acttccttga caatgtggta aatcttatca gggtcacgga gccacttggc
     2941 accagcctcg tttttgacaa ctttattaac agggcagccc atattaatat caacaatatc
     3001 ggccttggta ttggtttgaa tgaaatcagc tgcacgtttc aacccttctg cgtcaccacc
     3061 aaataattga atggacatag ggtgctcatt ctcgtcaata tggagcatgt gaagggtctt
     3121 ttcgttgttg tagagcagcc ctttttcgga aatcatttcc ataaccacca aaccagcacc
     3181 aaattctttg gcgatggtac gaaaggcaga attggtcacg ccagccatag gggcaagtac
     3241 agtgcggtgg gggatttcca catcaccaat cctaaaggaa gaattaagct ttgtcattga
     3301 tgagggcctc caaatcattt tcattaaatt ggtacttggt accacaaaat tggcaaacaa
     3361 tctcagcacc cttatcttcg tcaatcatgg cctgtaaatc tgcttttggc aaagtcatta
     3421 gggctgcttc aaaacgttct ctagaacagt cacactgaaa actcagaggt tcttcagaaa
     3481 gacgcttgta aggttcatct ccatagatgg cctccaaaag ggcatccaca tgatttttgg
     3541 aagctagtag gtgagaaatg gcaggcattt cttgcaggcg tttttcatag cgagcaatct
     3601 cttcttcaga agctcctggt agcacctgca ccataaagcc accagcaacc ttgaccttat
     3661 cattttcatc taaaagcaca ttaagtccga ttgcagatgg tgtttgctct gattcggtta
     3721 agtagtaggc aaaatcctca ccaatttccc cagtaataag aggcgttgtt gaggtgtaag
     3781 ggttacctgt gccataatca ataatcgtca caaaatgacc attccccata aaaggcccaa
     3841 caaggacttc acctgttgcg gtctttttaa tatcgacacc agtattttga atgtaacctt
     3901 tcacatgtcc tttggtatct gccacagaga taatgtgtcc aaaagaagaa tcaccaatca
     3961 ctttgacggt gattttacta tcgccttttt gattagccgc caaaatctgg ttggcgatca
     4021 aggtacggcc aagcgcaaca gtagacgatg acagcgtgtt gtgtttttct tgagctaatg
     4081 caaccgtctc agtgctatca aggacataag ccctaaaagc ccctgattgt gctattgatt
     4141 taattatttt atccataact tagattatag cataaagtgt aaaacttgta ttgacaggat
     4201 tgcttgaaaa atcaatacag atagacagta aaaaatccca gagaatgact cgggattaag
     4261 ttaaggtgtg gtctaaagtc gcgcaatgtg gtgacatggt gcttatgtta attgcttggt
     4321 taaatcagct tggaattttt cctctttaac ttgatacatc aattcttgga tagacagaat
     4381 cgattcatca aaagatattt cagcaacagc aattcctttt gtaagttcat ggtgaacaaa
     4441 aggaatcagt tgactgtgag tgatgaccaa atctggcttt aaatcaggaa tttgataaac
     4501 attatcttgc aatagataat aggaatgaaa atcaatgctt ttatccgaga aataccttgg
     4561 aaaagaatcc gttaggagat gagcattgat aaaattactg gctacgaaaa caacaactaa
     4621 aggaggttgg atatttctta gactttgctc gatatagtga caaaaaagat gaaaatgctt
     4681 atggttcaag tcacgcttac caggaagttt ggccatccac tcttcgacaa ttaactttaa
     4741 ggacgtatag agtttttggt ttcctttata gtacggagaa acgaataagt tggtctcagg
     4801 aataaaatgt tgcagattaa acaagaatga ttttgaaaaa aacataagag cttttactaa
     4861 actagccttt tgctctttta ggttaggtaa aagagtgatg ataggattta aaagcaggcg
     4921 aaaagtatca ttttcttcaa aaagttgaca acattgtctg atatgctcag gtgtccattg
     4981 taagctcgca aaagaattat tagcggtgat ataaattaaa tagaggtagt ccaaatctcc
     5041 tgctgaaaag tttagttggc agtaagtttc gataatatca tggctacttt ttttcaaaga
     5101 atcatagaca aaaagttttt ttaattgttg aaaaattctg gtttggggaa tagttaccga
     5161 aaattgatgc cgcttccacg ataaagccaa taaaatgtca tagaaagaaa acgattccga
     5221 taaccaagga gaggttttaa ggtgggtgga actatgggat aaaaagctat gaatagtgtt
     5281 tttgtcttgc tgcgtcaagt cataaacttt aatgccaaac ttactatata gcagagcgat
     5341 gaggtaacgg atgcgatatt cctcaccgac aatcttgttc ttagagagtt ttaattcaaa
     5401 gtttcttaat aaaggaatca atgcttcgcg catccgataa gctgaggagt ttgataaaaa
     5461 atgacttctt gcaaaatccg taaggggacg agagtgggaa ccatttttta ttaaaaacgc
     5521 tagtaattgt aagacattag atgatgcata gagttggtaa agataagttt ctttaaaagg
     5581 atgtgtaaat tggcaagata tcatcctttt ttggatggtc atagacagac taccagggaa
     5641 aaaggcattc agttcctcac aataatggtt tagttgtaaa aaggttaagc cagttttttc
     5701 tgctacctca gttattggca aataagatgt cttaaaaaac aagataatta actgacattt
     5761 tgattcgatt gatgattcca agtatttttc tatcaaggaa agggacctct cttcctaaac
     5821 ttagaaagcc aaataagtct ttgataagtg tattctccta tttcaattct atctaagcta
     5881 aaaatagaaa tcaaatctaa tggtttttgg tgaaaagtcg aaaccttttg aaatagcttt
     5941 aaagcaatat tttctcaaaa aatcattttc aaaaacatta atttggtgaa aaatataatc
     6001 aaaaaattgt ctttcttgac aataacgtgg taagctcata tatgtttttg agaggagaga
     6061 aaatgaataa caaaatattt ttgaataaag aagccggttt tttggcacac acaaaaagaa
     6121 aaaggcgatt tgctgtcact ttagtgggag tcttttttat gcttttggca tgcgcgggtg
     6181 ctatcggttt tggtcaagta gcctatgctg cggatgagaa gactgtgccg aattttaaaa
     6241 gcccagatcc agattatccc tggtatggtt atgatgctta tactggagcg tttttaagat
     6301 atcatgatct tagagtcaat ttaaacggaa gtcggagcta ccaagtttat tgttttaata
     6361 ttaaaaaaaa ctatcctcga ccttttacga gtagtgataa aaaatggtat aaaagattag
     6421 agggaactgc tgaaactttt aaagttcatg ctatggctcc cagagtaggg ggagaggaac
     6481 taactaaaaa attacggagc gttatgtata acggctaccc taatgacggc aataatatta
     6541 tgaaaggttt ggagccttca aatgctatag aagtaacaca atacgcagtt tggtactatt
     6601 cagataagtc acagtatgaa attgagacat tatggaagtc tgaagcgaaa gaaggtaaga
     6661 ttagtgagtc acaagttact ttaatgcgtg aagccctgag gaaactaatt tcctctgatg
     6721 aagatttggt taaacaagtt ccatcgaact ttaaacttag tacttttgag tcttctgata
     6781 agtcctacca aaatctttta agtgctgagt ttgtacctga tgatccccct aaacctggtg
     6841 atacgtcaga acataatcct aaaactcccg agttggatgg tagtcctatt cccgaagacc
     6901 caaaacatcc agatgataat ttagaaccta cattgccgcc agtgatgcta gatggtgaag
     6961 aagttccaga agttccaagc gagagcttag aacctgcgct tcccccattg atgccagagc
     7021 tagatggtga agaagtccca gaagttccaa gcgagagctt agaacctgcg cttcccccat
     7081 tgatgccaga gctagatggt gaagaagtcc cagaagttcc aagcgagagc ttagaacctg
     7141 cgcttccccc attgatgcca gagttagatg gtcaagaagt ccctgaaaaa cctagtgttg
     7201 acttacctat tgaagttcct cgttatgagt ttaacaataa agaccagtca cctctagcgg
     7261 gtgagtctgg tgagacggag tatattaccg aagtctatgg aaatcaacag aaccctgttg
     7321 atattgataa aaaacttccg aatgaaacag gtttttcagg aaatatggtt gagacagaag
     7381 atacgaaaga gccagaagtg ttgatgggag gtcaaagtga gtctgttgaa tttactaaag
     7441 acactcaaac aggcatgagt ggtcaaacaa ctcctcaggt tgagacagaa gacacgaaag
     7501 agccaggagt attgatggga ggtcaaagtg agtctgttga atttactaaa gacactcaaa
     7561 caggcatgag cggtttcagt gaaacagtga ccattgttga agatacgcgt ccgaagttag
     7621 tgttccattt tgacaataat gagcccaaag tggaagagaa tcgggaaaag cctacaaaaa
     7681 atataacacc tatccttcct gcaacaggag atattggaaa tgttttggcc tttcttggaa
     7741 tccttatttt gtcagtactt tctattttta gccttttaaa aaacaaacaa aacaataaag
     7801 tctgacgtaa aagtgttcca taagaaataa tgcgcaaata tgctgaatag tggataactg
     7861 ctacagtcaa gacaagtgtt aattcaaaag gtactactta tacatctgat aataataagg
     7921 tgacagataa ttcagagcaa gtaggatggc taaaaaatga tgtattctat gcataataga
     7981 cttacaagaa atcaagttat tgttaaacgt cgtaaagctt aacgctttca aaagtcgaaa
     8041 tcaatcgaga ctgtctttag gcagtctcga tttttattga taaggaactg caatgacaga
     8101 aagactaaaa aatctaggga tactcttatt atttttattg ggaacagcca tttttcttta
     8161 ccctacgcta agtagtcagt ggaatgccta ccgtgatcgt caattgttat caacttatca
     8221 caaacaagtg actcaaaaga agccctcaga aatggaggag gtgtggcaaa aggctaaggc
     8281 gtataacgct aggcttggga ttcagcccgt tccagatgct ttttcattta gggatggcat
     8341 tcatgataaa aactacgaaa gcctacttca aattgaaaac aatgatatta tgggatatgt
     8401 tgaagtgccc tctattaaag tgacgttacc catataccac tatacgacag acgaggtgct
     8461 aactaaggga gcaggtcact tagttggaag tgccttacca gttggtggcg acgggactca
     8521 cacggttatt tctgctcata gaggccttcc atcggcagaa atgtttacta atttgaattt
     8581 agtgaaaagg ggagatactt tttactttcg tgtgttaaat aaagtattgg cttataaagt
     8641 tgatcagata ctaactgttg aaccagatca agtcacatct ctttctgggg taatgggaaa
     8701 agattatgct actctggtta cctgcacacc atatggtgtt aataccaagc gtctattggt
     8761 cagaggtcat agaattgcat atcattacaa aaagtatcaa caggctaaaa aagcaatgaa
     8821 actcgtggat aaatcaagaa tgtggacaga agtagtatgt gctgctttcg gggtggttat
     8881 agctattatt ttagtattta tgtattcaaa gatgactgat aaaaaagtga ttaaaaacta
     8941 acttgccctg aaccttcttt atcgtattgc aactgtcata aaagtaaaat aatctggtag
     9001 ttactatccc ctttgatgaa gtttaattaa atctcatcaa gagaaaaata ttaggcgatt
     9061 acaaatgatt ttcccaaaaa ttattttttg gaacgcataa tatggaaaaa aagatgataa
     9121 gaacaactgt tttaaacatc aaataaatga tatagtagct atgaaatagc attgtgactg
     9181 tccggtaaag ggatgggact gaagatttta ttcagaagga gagggaatga ataacaaaaa
     9241 attgcaaaag aagcaagatg ctcctcgggt atcaaacaga aagccaaaac aattaactgt
     9301 cactttagtg ggagtatttt taatgctctt agtattgata ggatttgaag gaaaagttcg
     9361 agcagctcac gaattggttg aggtaccagt acctattttt cacaatccag atccacagtc
     9421 agactatcag tggtatggct atgaagccta tacagggggg tatccgaagt atgatttatt
     9481 caagacatac tatcatgatc tgagagtgaa tttgcatggg agtaaaagtt atcaagtata
     9541 ttgctttaac gttcacaaac actatccccg ttcctcccag agttttgatc ggaagtggta
     9601 taaaaaatta gatggaactg ctgagaattt tgattctctt gctatggaac caagagttcg
     9661 taaagaggag ttaaccaaaa aactccgagc tgttatgtat aatgcctatc ctaatgatgc
     9721 taatggcatt atgaaagatt tagagccttt aaatgctatt aaagtcactc aagaggctgt
     9781 atggtactat tctgattcag ctcaaattaa tccggatgaa agttttaaaa cagaagctca
     9841 aagtaatggt attaatgacc agcagttagg cttaatgcga aaagctttaa aagaactaat
     9901 tgatccaaac ttagggtcaa aatattcgaa taaaactcca tcaggttatc ggttaaatgt
     9961 atttgaatct catgataaga ctttccaaaa tcttttgagt gctgagtatg ttccggatac
    10021 tcccccaaaa ccaggagaag agcctccggc taaaactgaa aaaacatcag tcattatcag
    10081 aaaatatgcg gaaggtgact actctaaact tctagaggga gcaactttgc gtttaacagg
    10141 ggaagatatc ccagattttc aagaaaaagt cttccaaagt aatggaacag gagaaaagat
    10201 tgaattatca aatgggactt ataccttaac agaaacatca tctccagatg gatataaaat
    10261 tgcggagccg attaagttta gagtagtgaa taaaaaagta tttatcgtcc aaaaagatgg
    10321 ttctcaagtg gaaaatccaa acaaagaagt aggttctcca tatactatag aggcatacaa
    10381 tgattttgat gaatttggtt tactgtcaac acaaaattat gcgaaatttt attatggaaa
    10441 aaactatgat ggcagttcac aaattgttta ttgcttcaat gccaacttga aatccccacc
    10501 tgactcggaa gatcatggtg ctacaataaa tcctgacttt acgactggtg atattaggta
    10561 tagtcatatt gctggttcag atttgataaa atacgctaat acagctaggg atgaagatcc
    10621 tcaattattt ttaaaacacg taaaaaaagt aattgaaaat gggtatcata aaaaaggtca
    10681 agctattcca tataacggtc tgactgaggc acagtttcgc gcggctactc aactggcaat
    10741 ttattatttt acagatagtg ttgacttaac taaggataga ttgaaagact tccatggatt
    10801 tggagatatg aatgatcaaa ctttgggtgt agctaaaaaa attgtagaat acgctttgag
    10861 tgatgaagat tcaaaactaa caaatcttga tttcttcgta cctaataata gcaaatacca
    10921 atctcttatt gggacagaat accatccaga tgatttggtt gacgtgattc gtatggaaga
    10981 taaaaagcaa gaagttattc cagtaattca tagtttgacg gtgaaaaaaa cagtagtcgg
    11041 tgagttggga gataagacta aaggcttcca atttgaactt gagttgaaag ataaaactgg
    11101 acagcctatt gtcgacgctc taaaaactaa taatcaagat ttagtagcta aagatgggaa
    11161 atattcattt aatctaaagc atggtgacac cataagaata gaaggattac cgacgggata
    11221 ttcttatact ctgaaagaga ctgaagctaa ggattatata gtaaccgttg ataacaaagt
    11281 tagtcaagaa gctcaatcag caagtgagaa tgtcacagca gacaaagaag tcacttttga
    11341 aaaccgtaaa gatcttgtcc caccaactgg ttttactact gatggtggaa cctatctgtg
    11401 gttattattg cttgtcccat ttggtttgtt agtgtggttc tttggtcgta aaggactaaa
    11461 aaatgactaa ttacctaaat cgcttaaatg agaatccact atttaaagct ttcatacggt
    11521 tagtacttaa gatttctatt attggatttc taggttacat tctatttcag tatgtttttg
    11581 gcgtcatgat tgttaacaca aatcagatga gtcctgctgt aagtgctggt gatggagtct
    11641 tatattatcg tttgactgat cgctatcata ttaatgatgt ggtggtctat gaggttgata
    11701 acactttgaa agttggtcga attgccgctc aagctggcga tgaggttaat tttacgcaag
    11761 aaggaggact gttgattaat gggcatccac cagaaaaaga ggtcccttac ctgacgtatc
    11821 ctcactcaag tggcccaaat tttccctata aagttcctac gggtacgtat ttcatattga
    11881 atgattatcg tgaagaacgt ttggacagtc gttattatgg ggcgttaccc atcaatcaaa
    11941 tcaaagggaa aatctcaact ctattaagag tgagaggaat ttaaaaaaat ttggagagag
    12001 agaaaatgaa aaaaaataaa ttattacttg ctactgcaat cttagcaact gctttaggaa
    12061 cagcttcttt aaatcaaaac gtaaaatctg agacggcagg agtgtccgaa aatgcaaaat
    12121 taatagtaaa aaagacattt gactcttata cagacaatga agttttaatg ccaaaagctg
    12181 attatacttt taaagtagag gcagatagtt ctgcaactga taaaacgaaa gatggtttag
    12241 agattaagcc aggagtaact gaaggtttaa ctaccgagca gacaattgct tatgataact
    12301 cagttaaacc atcggataaa tctaagactg caacttttga tttttcaacc gtaaaatttc
    12361 cagaagttgg tgtttatcgt tatacggttt cagaaataga tagcaaagtt agtgggatta
    12421 aatacgatac caaaacatgg atagtggatg tttatgttgt taatgatggt aatggaggat
    12481 ttaaggcacg gtatattgta tctaaagaga agggacaaaa tgataaaaag cctgttgttt
    12541 ttgaaaactc ctttaagaca acttctttaa aggtagaaaa acaggtgact ggtaatacag
    12601 gagaactaaa gaaagacttc aatttcactt tgacaattaa tcctaatgat aatttcgtag
    12661 ctggacaagt tattaaatta gagaaaggtg gaattaaagc tgatgttaag attggtgagc
    12721 cgtataaatt tgctctaaag aatggtgaga aagtaacttt atcaaaatta ccagtaggtg
    12781 ttacttatag catcattgaa gatgaggcag ataaagacgg atacacaact aatgccaaaa
    12841 ttacagatgg tacggcagcg cctgtggaat ataagttagg aaatcaacaa ttggctgacg
    12901 aatctgctga cgaaatcgtt gtcacaaata accgtgacac tcaagttcca actggtgttg
    12961 taggcaccct tgctccattt gcagttctta gcattgtggc tattggtgga gttatctata
    13021 ttacaaaacg taaaaaagct taagtgatta gtcaaagaat gatgatgaca attgtacagg
    13081 ttatcaataa agccattgat actctcattc ttatcttttg tttagtcgta ctatttttag
    13141 ctggttttgg tttgtgggat tcttatcatc tctatcaaca agcagacgct tctaatttca
    13201 aaaaatttaa aacagctcaa caacagccta aatttgaaga cttgttagct ttgaatgagg
    13261 atgtcattgg ttggttaaat atcccaggga ctcatattga ttatcctcta gttcagggaa
    13321 aaacgaattt agagtatatt aataaagcag ttgatggcag tgttgccatg tctggtagtt
    13381 tatttttaga tacacggaat cataataatt ttacggacga ttactctctg atttatggcc
    13441 atcatatggc aggtaatgcc atgtttggcg aaattccaaa atttttaaaa aaggattttt
    13501 tcaacaaaca taataaagct atcattgaaa caaaagagag aaaaaaacta accgtcacta
    13561 tttttgcttg tctcaagaca gatgcctttg accagttagt ttttaatcct aatgctatta
    13621 ccaatcaaga ccaacaaagg cagcttgttg attatatcag taaaagatca aaacaattta
    13681 aacctgttaa attgaagcat catacaaagt tcgttgcttt ttcaacgtgt gaaaattttt
    13741 ctactgacaa tcgtgttatc gttgtcggta ctattcaaga ataacgaaag gaggagactt
    13801 ttgagaaaat attggaaaat gttattttct gtcgtaatga tattaaccat gctggccttt
    13861 aatcagact
//
LOCUS       DAUVUY010000053        13513 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.053, whole genome shotgun sequence.
ACCESSION   DAUVUY010000053 DAUVUY010000000
VERSION     DAUVUY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13513
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.053"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..354)
                     /gene="rplN"
                     /locus_tag="VOX58_001265"
     CDS             complement(<1..354)
                     /gene="rplN"
                     /locus_tag="VOX58_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HES5878492.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLA"
     gene            complement(379..639)
                     /gene="rpsQ"
                     /locus_tag="VOX58_001266"
     CDS             complement(379..639)
                     /gene="rpsQ"
                     /locus_tag="VOX58_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HES5878493.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(665..871)
                     /locus_tag="VOX58_001267"
     CDS             complement(665..871)
                     /locus_tag="VOX58_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HES5878494.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(881..1294)
                     /gene="rplP"
                     /locus_tag="VOX58_001268"
     CDS             complement(881..1294)
                     /gene="rplP"
                     /locus_tag="VOX58_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HES5878495.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(1298..1951)
                     /gene="rpsC"
                     /locus_tag="VOX58_001269"
     CDS             complement(1298..1951)
                     /gene="rpsC"
                     /locus_tag="VOX58_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HES5878496.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(1964..2308)
                     /gene="rplV"
                     /locus_tag="VOX58_001270"
     CDS             complement(1964..2308)
                     /gene="rplV"
                     /locus_tag="VOX58_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HES5878497.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(2324..2602)
                     /gene="rpsS"
                     /locus_tag="VOX58_001271"
     CDS             complement(2324..2602)
                     /gene="rpsS"
                     /locus_tag="VOX58_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HES5878498.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(2742..3575)
                     /gene="rplB"
                     /locus_tag="VOX58_001272"
     CDS             complement(2742..3575)
                     /gene="rplB"
                     /locus_tag="VOX58_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HES5878499.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(3593..3889)
                     /locus_tag="VOX58_001273"
     CDS             complement(3593..3889)
                     /locus_tag="VOX58_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HES5878500.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(3889..4512)
                     /gene="rplD"
                     /locus_tag="VOX58_001274"
     CDS             complement(3889..4512)
                     /gene="rplD"
                     /locus_tag="VOX58_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HES5878501.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(4536..5162)
                     /gene="rplC"
                     /locus_tag="VOX58_001275"
     CDS             complement(4536..5162)
                     /gene="rplC"
                     /locus_tag="VOX58_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HES5878502.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(5410..5718)
                     /gene="rpsJ"
                     /locus_tag="VOX58_001276"
     CDS             complement(5410..5718)
                     /gene="rpsJ"
                     /locus_tag="VOX58_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HES5878503.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(5917..7206)
                     /locus_tag="VOX58_001277"
     CDS             complement(5917..7206)
                     /locus_tag="VOX58_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HES5878504.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(7594..8610)
                     /gene="adhP"
                     /locus_tag="VOX58_001278"
     CDS             complement(7594..8610)
                     /gene="adhP"
                     /locus_tag="VOX58_001278"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HES5878505.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVAPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(8862..11504)
                     /gene="adhE"
                     /locus_tag="VOX58_001279"
                     /gene_synonym="adhC"
     CDS             complement(8862..11504)
                     /gene="adhE"
                     /locus_tag="VOX58_001279"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HES5878506.1"
                     /translation="MTEGHNTVETTSVSVTINALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKIADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
     gene            complement(11813..>13513)
                     /locus_tag="VOX58_001280"
     CDS             complement(11813..>13513)
                     /locus_tag="VOX58_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HES5878507.1"
                     /translation="VFPGGFIGVDIFFTFSGYLITALLIDEYTKKESIDIIGFLKRRF
                     YRIVPPLVLMILLTIPFTFLIKKDFIANIGSQITAVLGFTTNIYEILTGSSYESQFIP
                     HLFVHTWSLAIEVHFYLLWGVFVWLLARRKETQKQLRGLLFLISLGIFAISFLSMFIR
                     SFMTSNYSLIYFSSLSHSFPFFLGAMFATITGINETTVRFQKNVRLWPRQYVLAAMIG
                     AFTLLLVLTVTLDFNHITTYLFGFALASLFASIMIYAARVLHEQTPDVQEPKAITYIA
                     DISYGIYLFHWPFYIIFSQLMSHILAVILTVFFSILFATVSYYIVEPLVQGRKPNLLG
                     LEIDCSPYYKWIVGGSLALALLTLGTCMIAPKVGKFEKQLLVSSLQQAQSNMERTHTL
                     AAGDANALSDVGIIGDSVALRSSAAFSKIMPQAQLDAAVSRNFKEAFDLFNNQIKSKS
                     LSKTVVLAVGVNSLDNYSQAVQSFIEALPKGHRLVLVSPYNAKNASQVAEARDYGLKL
                     SKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSEDSQGAELYVSTIQTAVEKSAKKPAK
                     "
BASE COUNT         4009 a         2877 c         2453 g         4174 t
ORIGIN      
        1 tgcaagtgat acgatcttca tgtagccacc ctcacgcaat tcgcgtgcaa ctgggccaaa
       61 gatacgagtt ccgcgaggag ttttatcatc acggatgatt acagcagcat tgtcgtcaaa
      121 tttgatgtat gaaccgtctg gacggcgtgc accagttttt gtacgaacga taacagcttt
      181 aaccacatca ccttttttaa ctgctccacc aggagtagct tgttttacag aagcaacgat
      241 tacgtcaccg atgttagcga atttacgtcc tgaaccacca agtactttga tagtcaagat
      301 ctcacgagca ccgctattat cagcaacttt caagcgagtt tcttgttgaa tcatttcaat
      361 tttctccttt tagtttgatt agataataac agctttttcc actacttcca caagacggaa
      421 acgttttgta gctgaaagtg ggcgagtttc catgatacga acgatatcgc cttctttagc
      481 aacgttgttt tcatcatgtg ctttatattt tttagaatag ttgatacgtt taccatagac
      541 tgggtggtta cgtttagttt caactacaac tgtgattgtt ttgtccatct tgtcagacac
      601 tacgcgtccg taaagagttt tacgttgatt acgttccatt tagaatttct cctttcccaa
      661 tctattattt catttctgat tgcacagttt taacacgtgc aatttgtttc ttaacttcgt
      721 caaggcgagc agtcttttca agttgacctg ctgcagcttg gaaacgaaga tcaaacaatt
      781 cttttttgag ttcgttttct ttcttagcaa gttcttcttg agacaatcca cgaagctctt
      841 taacaaaatc tttaatttct tgaagtttca tgtcttctcc ttattctgct tcacgtttta
      901 cgaatttgca cttaactggt aatttgtggc tagcaagacg taatgcttcg cgagcaattt
      961 cttcagaaac accagcaatt tcaaacatca ctttaccacg tttaactggt gctacccaac
     1021 cttcaggtgc ccctttacca gaacccatac gtacaccgat agcttttgca gtgtatgatt
     1081 tgtgtgggaa gattttaatc caaactttac caccacgttt catgtaacga gtcatcgcaa
     1141 tacgggcagc ttcgatttga cggtttgtaa tccagtggct agttgtggct tgaagtccgt
     1201 attcaccgaa tgaaacttct ttaccacctt tagcctcacc gcgcattttt ccacggaatt
     1261 cgcgacggtg tttaacacgt ttaggtacta acatttgtta tttgcctcct ttagtgtttt
     1321 tacgagctgg aagaacttct ccacggtaaa tccaaacttt aacgccaagt ttaccataag
     1381 ttgtgtcagc ttcttcccaa gcatagtcga tatcagcgcg aagcgtgtga agtggaacag
     1441 ttccttctga ataaccttca gcacgagcga tatcagcacc gttcaaacga ccagaaactt
     1501 gagttttaat ccctttagct cctgcacgca ttgtacgttg gattgcttgt ttttgagcac
     1561 gacggaaagc aacacgttgc tcaagttgac gagcaatgtt ttcaccaaca aggtgagcat
     1621 ctaaatcagg ttgtttgatt tcgatgatgt tgatgtgtac ttgttttcca gttaatttgt
     1681 taagttgacc acgaagagcg tcaacgtttg cgccaccttt accgataacc atacctggtt
     1741 tagcagtgtg aagtgaaaca ataactttgt ttactgcacg ttcaatttca atagttgaaa
     1801 ctgatgcgtc agctaattct ttattaatga atttacggat tgcaagatct tcatgaaggt
     1861 aatccgcgta ttctttttca gcataccatt tcgcatccca gtcacggatg attccgacac
     1921 gcataccaat tggatgtact ttttgaccca cgattttacc tccttatttt tctgatacaa
     1981 ctacagttac gtgagttgta cgtttgttga ttggtgaagc tgaacctttc gcacgtggac
     2041 ggaaacgttt cattgttggt ccttcgttag cgaatgtttc agatactacc aagtttgctt
     2101 tttccaaacc aaagttgttt tctgcgttag caattgctga gttaagagtt ttctcaataa
     2161 cacgagctgc tttgtttgga gtgaatttta agattgcgat tgcgtcagca actttcttac
     2221 cacggataag atcaagtact aaacgtgttt tacgaggtga aacacgcact gtacgagcca
     2281 ttgctttagc tgaagtaatt tctgccattg tgtcctcccc ctattaacga cgtgttttct
     2341 tgtcgtcagc tgcgtgacct ttgtaagtac gcgttggtgc aaattcacca agtttgtgac
     2401 ctaccatgtc ttcttggatg taaacaggta catgtttacg tccgtcataa actgcgattg
     2461 tatatccgat gaaacttggg aaaatcgttg aacgacgtga ccaagttttg attacttttt
     2521 tcttttcgtc gtttgcttga gcttcaactt ttttcatcaa atgctcatcg acgaaaggtc
     2581 cttttttaag actacgtccc attttgtagt tttctccttt aaataatgta ccacagcggc
     2641 ttgagttcaa gagcaagact ccaaaactct accgagttgg cggaataaat agcaaagcta
     2701 ttgtaacgct atttcagcaa gttttaagca acttactaag attatttttc gttacgacga
     2761 cgaacgataa gtttgtctga tttagctttc ttgttacgag ttttaagacc aagcgctggt
     2821 ttaccccatg gagttgatgg cgctttacgt ccaactggtg ctttaccttc accaccaccg
     2881 tgtgggtgat cgttagggtt cattacagaa ccacgaactg ttgggcggat acctttccaa
     2941 cggctacgtc ctgccttacc aatgttaaca agtgattgtt gttcgttacc aacagtaccg
     3001 atagttgcac ggcatgtacc aagaatcata cgaacttcgc ctgattgaag acgaacaaga
     3061 acgtatttac cttcttgacc aagtacttga gcagaagctc cagctgcacg aacaagttct
     3121 ccacctttac caggtttcaa ctcaatattg tgaacaactg taccgacagg aatgtttgct
     3181 aatggaagtg cgttaccaac tttgatatct gcatctggac cagaaacaat acgttgacct
     3241 acttcaagac ctttaggtgc aatgatgtaa gctttcacac cgtcagtgta atgtacaagt
     3301 gcgatgtttg cagtacggtt tggatcatat tcgattgttt taacaactgc ttcaacgcca
     3361 tctttgttac gtttgaagtc gatcaaacgg taatgacgtt tgtgtccacc accttggtga
     3421 cgaactgtga tgcgaccatt gttgttacga ccagctttgc tcttaagaga aacaagcaat
     3481 gatttctcag gcgtgcttgt tgtgatttcc gcgaaatcca aagaagtcat gttacgacgg
     3541 ccatttgtcg ttggtttata aactttaata cccacgttat ttcctcctta gattattcag
     3601 cttcagctgc gaacaactcg attgctttag aatcagctgt aagagtgatg atagcttttt
     3661 tagtttttga agtgaaacct gtgtaacgac caacgcgttt tgcttttggt ttaacgttaa
     3721 cagtgtttac acttgcaact ttaactccgt caaaagcagc ttcaacagct tgtttgatca
     3781 aaagtttgtg tgcacgagta tcaacttcga aagtgtattt gcctgcttca agagcaatca
     3841 ttgacttctc agtgataact ggttttttga ttacgtcgta caaattcatt atgcaagaac
     3901 ctcctcaatt gtagagattg cttctttagt aacaagaagt ttgtctgcgt tcacgatatc
     3961 aagaacactt gcagttgctg cagttgcaac agttacgttt ggaaggttac gtgcagaaag
     4021 tgctgcaaat tcatttcctt cttcaacaag aacaagtact tttgtatcaa tgctaagagc
     4081 tgaaagcact tttgcaaatt cagcagtttt tggtgctgca aatgaaaggc cttctacagc
     4141 tacaaattta tcttcagcaa cttttgctga gtaaactgat ttcaacgcaa ggcgacgaac
     4201 tttttgtgga agtttgtatc cgtatgaacg gggagttggt ccaaagacaa caccaccacc
     4261 acgccattgt ggtgagcgga tagaaccttg acgagcgcgt ccagttcctt tttgacgcca
     4321 tggtttacgt ccaccacctg atactgctga acggttttta accgcatgag taccttggcg
     4381 aaggctagcg cgttggctaa ttacaacatc aaaaacaact gattcgtttg gttcgatacc
     4441 gaagatagcg tcgtttaatt caactgagct aacttcttta ccagtttggt caaatagttt
     4501 tacgtttgcc attttaactg ttttctcctt tcttattatt tagcagcttt aactgctgac
     4561 ttgatagtga taagagattt cttagcacct ggtacgttac ctttaataag gataacgttc
     4621 ttttctggga taacttgtac aatttcaagg ttttgaactg ttacacggtt accacccata
     4681 cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg caacaggtcc catagaacct
     4741 gggcgacgat ggtaacgaga accgtgagcc ataggaccac gtgattgacc atggcgtttg
     4801 ataacacctt ggaaaccttt accttttgat gtccctgtga cgtcaacaac atcaccagct
     4861 tcaaattgtt ctacagataa ttctgcacca acttctaagc cttcaatgtt tttgaattca
     4921 cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat ggcctttggc aggtttgtta
     4981 ctcaagactt cacgtttgtc atcaaaacca acctgaactg cttcataacc gtctgtttca
     5041 acagttttaa cttgaagcac aacgtttgga gttgcttcaa tgacagtaac agggatgaat
     5101 tcgcctgatt cagtgaagat ttgagtcatt cccacttttt tccctaagat tccttttgtc
     5161 atgagaaaat atttcctttt cttaataata tttcaaaaag ttttttatga gcgttttttg
     5221 tgctcaaaaa agttttttac gagcgttttt tgtgctcata gcaagataca aagagcgtca
     5281 aactcaaaat aaaaatagaa aatctaacac aaaaacttct gtttcgagga agatttatct
     5341 ttttcatgta gagtttagct cgtgttcaag ttccatcaaa cactaactgt acttgcaaaa
     5401 tctcaccgat taaagtttga tttctacgtt gacaccactt ggaagatcaa gtttcatcaa
     5461 agcatcaaca gttttttgtg ttggattgat gatgtctacc aaacgtttgt gtgtacgcat
     5521 ttcaaattgt tcgcgagaat ctttgtattt gtgagtcgca cgaataattg tgtaaagact
     5581 gcgttcagtt ggaagtggaa ctggtccagc aactgtagca cctgtacgtg ttgcagtttc
     5641 aacgattttt tctgccgctg tatcaagtgt acggtgttcg tacgctttca aacggatacg
     5701 gatttttttg tttgccatct tttctcctct cgtctattta cgataatagg ctagctcctc
     5761 aagaaaaccg acacctgttg cgtggcaatg caaccgagcg tgtcgcaacc tcttgtatca
     5821 ttgctaaagc tgtattttta cagcaccaca atataataac agaatcattt agtcattgca
     5881 agctttttag ggcatttttt caaaaaaact accttcctaa tgaaaggtaa tttcttggta
     5941 ctttttaaat tgtttagaga gaataaacca ccgactggta ttatctaaca ctgtcgccat
     6001 ccagacccct atgagtccat attgccaaac cacaccaatc acataaccaa ggccaatgcg
     6061 aatcacccac attcctatgg ttgttgcata gaagggaagc tttgcttttc ccaaaccctg
     6121 ccagacggct gtataaacta aagtgcctgc cgttgcggga gctcctagta aagaaaaaag
     6181 taaaacaacc atggcggacc tttgagcatc agtattctga gtaaatagcg gcaagagact
     6241 aggacctagc agataggtca aggctcccat aaccagcatc atgagggtgg ataaaataaa
     6301 agcttctcta ataatatacc ttatctcagt caccttaccg ccccccagtt gcctggccac
     6361 tagaataatt gttgccgttg ccatggctaa gccaggcatg taattaaact gagtcaaggt
     6421 ttctccaata gcattacctg ccaaggccgt tgtcccaaaa cgaacaacaa tgccaataat
     6481 taggacatct cctgctctca tcatcaaacg ttcaccagca gcaggtagtg ataaatcaaa
     6541 aataattttg tccaaaggtc tcatcaatcg tttcgcaacc tgtttaatag gaataaactg
     6601 acaaagtaaa aacacaccca ctaaacgaga taaaaccgtc gcccaagcaa caccaagaag
     6661 accaaatccc catacataga tagacaaggc agaaaaaatg gcattgagta cgtttattaa
     6721 taagctcact tgcataggaa tcttaggata cccttgagct cgaacaatag ctcctaagct
     6781 agttaaaagt cctagactaa caatcatacc gccgacaatg gatagatatt gccctccaac
     6841 caatgttaca gaagcttcag ctcctagcca ttctaacacc tgatgatgtc caacaactga
     6901 taacaaccct aagccgaccg aaaggaggag tgttacttgc aagacacctg ccatatagtt
     6961 taattgtttg ttctgattat tttctccaat acttcgagca atcagactag atacagcggc
     7021 tcccagcgcg ataaagaggg actgataaat actgataatg ttgttagcta tggagacacc
     7081 agagactgct acaagtccaa tttgagccac caggtaatta tcaaccattc ccattaacat
     7141 ctgtaaaata ttttctatca tagaaggcag agctaaagag aaaatttttc ttctattata
     7201 tatcatttat gtcccctaat aaaaaattct tgatcaaacg atttttttaa acacagtcac
     7261 tggactcatt cagttatttt gttccattaa tgaattcata aagtctacct taatagcaga
     7321 gtcccaagat acacgacact tagtgttaca gtaacaattg accacatgcc aatccttaac
     7381 atttacctaa cgtttataac taagaagacg aagaacaaaa ggactccaaa cgaatttttt
     7441 ctattatata atatattctt catttagcaa ctttcaatgt atcaaaatag cactctcgag
     7501 tcaaaaatgt tttctgatac tcgagagtgc tattttattt ggaatcaagt ttaagcaact
     7561 taggcttatc tcatatttaa tacatttgta tgtttagctg atgaaatcta agaccttgcg
     7621 cccttggatt aaaccacgtt ccatttcatc aaagacttct ggagcagtgt caactggaac
     7681 tttttcgaca accggagcaa ccagaccttc tgcaccaaag gcaaaggctt cttccaagtc
     7741 tttacgtgtc cctacaagtg atccgaccac tttgatacca tctaaaacag tttttacgat
     7801 agacagttcc atgtattctg atggaagacc tacggctaca actgttccac cggcacggac
     7861 actatcaatt gcttggttaa aagcaacttt agaaacagcc gttaccacaa caccatgtgc
     7921 tccaccggtt ttttcttgaa tatagcctgg aacatcttca atttctttgc cgttaactaa
     7981 aatatctgcg ccaacttctt tagctaactc aagtttatca ttattaatgt ccactgcgac
     8041 aacatgtgca ttaaagactt ttttagcgta ttgaacagcc agatttccaa ggccacctgc
     8101 cccaaagata acaatccatt ggcctggagc agcacctgcc tctttaatag ctttataagt
     8161 tgttacacca gcacaagtaa tggaagaagc ttgagccggg tctaaacctt ctggaacttt
     8221 aactgcataa tcagctgtta ctactgcata ttcactcatt cctccatcaa cactgtatcc
     8281 agcattttta acactgcgac aaagtgtctc acgtcctgta gtacagtatt cgcaatgacc
     8341 acatccttca aagaaccatg caatagagac acgatcacca acctttaagg atgttacgcc
     8401 ttctccaatt tcttctacta taccaatacc ttcgtgcccc aaaatacgtc ctggtacctg
     8461 accaaaatcg ccatgtgcaa cgtgtaaatc agtatgacaa acgccacagt attctacttt
     8521 aacaagcgct tcaccatgac caacatttgg aagatcatgc tcaacaactt cgacacctgt
     8581 actagcttga ttaacaacta cggctttcat atcagtcctc catctaataa cgacttattt
     8641 gtaatcggtt acaaataaat tgtacatctt ttcacaatta aagtcaacag aaacctgaaa
     8701 agtttctgaa acttaactgg ttaatcttat agctgtttgc ctatctgcaa aatacttaac
     8761 tacattttct gacactggat ataaatgata tatttagaaa ataaaatgat ttcttatgac
     8821 tttaatcata agaaatcatt tcctggtatc aacattaaaa atcacttacg tcgtcctgga
     8881 cgttcagcat aaccatagta agcatcttca ataatttctt gcatgtgatc aaccattgga
     8941 agacgtgggt tagcaggtga acattggtct tcataagcaa ggtaagccaa ttcacgagaa
     9001 tgttctttcc attcattttc gtcgatacct tgagctttga agttcatttg aataccaact
     9061 cggcaaccca gatcataaac agctttcgca taagattcca ctgcttcttc tggtgtagaa
     9121 gctggaagcc ctaacaattt cgcaatgtct tggtattttt catccgcacg gtagtaatta
     9181 tacttaggcc atgtcgccgt tttagctgga cgagttccat tatagcggat aacatacggc
     9241 aaaagaattg catttgtacg tccgtgtacg gtatggaatt gtgcgccaat tttatgcgcc
     9301 attgagtgag aaatacctaa gaatgcattg gcgaaagcca tacctgccat agttgacgca
     9361 ttgtgcatct tttcacgtgc ttcaaaatcg gctattttaa ctgatttttc aagattatca
     9421 aaaacaattt taatggcttg aagagctaaa ccatccgtaa agtcattggc catttgtgac
     9481 acgtaagctt cggttgcgtg tgtcaaaacg tccataccag tatcagcagc aataaagcct
     9541 ggaacagtca ataccaaagc tgggtcaaca atggctacag ttggtgttaa tgagtaatca
     9601 gcgattggat atttacgatt atttgctttg tcagagataa cggcaaatgg tgtcacctct
     9661 gaacctgttc cagaagttgt tgggatagca acaaattttg ttttcttacc aagttctggg
     9721 aatttgaacg cacgtttgcg gatatccatg aatttttgaa cgaggtcatg gaaatcaact
     9781 tctggttgct cgtagaagag ccacataact ttggctgcat ccataggaga tcccccgcca
     9841 agggcaataa tggtgtctgg tttgaaggta cgcattaatt cagtaccttt cataacagtt
     9901 gtaatgtctg gatctggttc tacttcagca aagatctgat aaacaacttt attgcgacga
     9961 agatcaagtt gttcaatgat acgatccaag aaaccaagct caaccatagc gtgatctgta
    10021 acaatcatga cacgttcaac gtcacgacat ttttgcaaat attgaattga atcacgttcg
    10081 aagtatgttt ttgaaggaac tttaaaccat tgcatattat ttctacgtct tcctactttt
    10141 ttgatgttca agagatttat agcactaaca ttatcaccaa ctgcgttacg tccatacgaa
    10201 ccacaaccaa gagtcaatga tggcaagaaa gcattgtaaa catccccgat accaccaaat
    10261 gtagaaggtg aattccagat gacgcggata gcacggattc tagttccaaa ttcttttgcc
    10321 aactctgcat ctgctgtatg aatggcagct gagtgaccga gtccattaaa ttcaaccatt
    10381 tgacgagctt tttcaacacc gtcttcacgt gattcagatt tcaaaacagc aatgactgga
    10441 gataattttt cacgtgttaa aggctcattt tctgaaactt ctttacattc tgcagcaaga
    10501 atattggttc cttctggaac agtaaatcca gcttgttctg caatccaagt agctggtttt
    10561 cctacaatat ttggatttaa ttttgcacca gcacagtttt tgctatttgc tttggcacca
    10621 aaacagaatt cttcaagaag ggctttttct tttttattta caaaataagt atggtaagat
    10681 ttgaattctg ctacaaaatc gtcgtagatt tctttatcga tgataacagc ttgttcagag
    10741 gcacagacca taccattatc aaatgattta gacatcacaa tatcatgagc ggcttgacgg
    10801 atattagctg atttttcaac ataagctgga acgttaccag cacctacccc aagggcaggt
    10861 ttcccacatg agtatgccgc tttaaccatc gcatttccac ctgtcgcaag gatagtggca
    10921 atgccgtcat gattcatcaa agcatttgtt gcttcaagtg atggtgtttc aacccactgc
    10981 acacaatttt caggagcacc agcggcaata gcagcatcac gaacaatacg agcggcatgt
    11041 gctgatgatt cttgtgctga tgggtgaaag gcaaaaataa ttggattacg tgtttttaac
    11101 gagatcaaag atttaaagat agctgttgaa gttgggttag ttgtgggtgt aatcccacag
    11161 ataacaccca ccggctcagc aattaatgtc aaacctgtca catcatcttc ttcaataatt
    11221 cctactgttt tttgatggcg catgttatta accacatgct cacaagcaaa tagatttttt
    11281 gttgctttat cttcaaagac accgcgacct gtttcttcat aagcatgttt agcaagttca
    11341 ccgtgagcat ccaaggcagc aacagaagct ttggctacga tataatcaac ttgttcttgg
    11401 tcaagcttac gcatctcttc aagagcagct aaaccttttt gaacaagagc attaattgtc
    11461 acagaaacag aggttgtttc aacagtatta tgtccttcag tcatttgaga ctcctttatc
    11521 ttgttaaaag tttcacaatt ttgtttacaa aattattata gcactttttg aaattttgta
    11581 aatcatttca caataaattt gtgaagtttt tttcaaatta tttctgaaat cctatggtat
    11641 caatattatt cattaactct ttttcagaaa tatatctaaa aaaagccgtt ttatttctga
    11701 aatcgctttc ttttaaaaac ctcaaagtac tactatttta ccatcatcat ttgttcacaa
    11761 gaaaaagcca gagcacaatg gatctgactt atctcttaga catttcttaa ctttatttag
    11821 ctggtttttt agctgacttc tcaacagcag tctggattgt ggagacatat agttctgctc
    11881 cttgactatc ttcactataa tgaacaccgt cactgccata ccaaatatct ggatgttcaa
    11941 cagcaacttt ataccagtct gcaatagtga catacttgta ttttttggac aatttaagac
    12001 catagtctct agcttctgca acctgactgg catttttggc attatatgga gaaaccaaaa
    12061 ctaaacgatg tccttttggc aatgcctcaa taaaagactg aactgcttga gaatagttgt
    12121 caagcgagtt aacacctact gctaagacaa cagtcttaga tagactctta gacttaattt
    12181 ggttattaaa taaatcgaaa gcttctttaa aattccgact gaccgctgca tccagttgcg
    12241 cttgtggcat aatttttgag aatgctgcac tagagcgtaa tgctacagaa tcaccaataa
    12301 tacctacgtc actcaaagca ttagcgtctc ctgcagctag tgtatgtgtc cgctccatat
    12361 tactttgagc ctgttgtagg gaactgacca acaattgttt ctcaaactta ccaactttag
    12421 gcgctatcat acaagttcct aaagttaata atgctaaggc gagagaccct cctacaatcc
    12481 acttatagta aggagaacag tcaatttcta aaccaagcag gtttggtttt ctcccttgga
    12541 ccaaaggttc cacaatatag taagacacag tggcaaacaa gattgaaaag aaaactgtta
    12601 gaataactgc caaaatatga gacattaatt ggctaaaaat aatgtaaaat ggccaatgga
    12661 agaggtaaat cccatagcta atatcagcta tataagtgat agctttaggc tcttgtacat
    12721 ctggtgtttg ctcatgaaga acacgcgctg cataaatcat aattgatgcg aataagctag
    12781 ccaaagcaaa gccaaataga taggtcgtga tatggttaaa atccaatgtg accgttagca
    12841 caagcaaaag tgtaaatgct ccaatcatag cagcaaggac atactggcgc ggccataaac
    12901 gtacattttt ttgaaatcga acagtagtct cgttaatacc tgttattgtg gcaaacatcg
    12961 cccctaaaaa gaagggaaaa ctgtgagaaa gactcgaaaa ataaatcagg gaataatttg
    13021 aggtcataaa agatcttata aacatactca agaaactaat agcaaatatt cccaaagaaa
    13081 tcaaaaacag tagtcctctc agttgtttct gagtctcctt gcgtctagct aataaccaaa
    13141 caaatacacc ccacaacaag taaaaatgaa cttcaatagc taagctccac gtatgaacaa
    13201 agagatgtgg aataaattgg ctttcataac tacttcccgt caatatttca taaatattag
    13261 tggtaaatcc taaaacagct gtaatctgac ttcctatatt agcaatgaaa tccttcttaa
    13321 ttaaaaacgt aaaaggaatg gtcaataaaa tcattaacac caaaggagga acaattcgat
    13381 aaaagcgtct ttttaaaaaa ccgataatat cgatagactc tttttttgtg tattcatcaa
    13441 tcaagagggc tgtgattaga tatcctgaaa aggtgaagaa aatatcaaca ccaataaaac
    13501 ctcctggaaa tac
//
LOCUS       DAUVUY010000054        12966 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.054, whole genome shotgun sequence.
ACCESSION   DAUVUY010000054 DAUVUY010000000
VERSION     DAUVUY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12966
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.054"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(42..1130)
                     /locus_tag="VOX58_001281"
     CDS             complement(42..1130)
                     /locus_tag="VOX58_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HES5878508.1"
                     /translation="MKIKSYKKENGETAYKFLLYAGYVNGKRKYIRREGFKTKQAARE
                     TLISLQAELDKPKSSMTFGVLTKQWLNEYEKTVQGSTYLKTERNINKHILPKLDKVKI
                     GDINPLLIQRLTEEWCNDLKYGGKILGLVRNILNLAVRYGYINNNPALPITPPKIKRK
                     RKMNNNFYTLDQLKQFLELVEKTDNIEKIALFRLLAFTGIRKGELLALTWDDLNGNTL
                     SINKAVTRTQVGLEIDVTKTKSSDRLISLDDETLEILQELHETFPTSTLMFQSESGGI
                     MTPSLPRKWLLQIIKGTDLPQITIHGFRHTHASLLFESGLSLKQVQHRLGHGDLQTTM
                     NVYTHITQSAIDDIGTKFNQFVTNKQLD"
     gene            complement(1380..2189)
                     /locus_tag="VOX58_001282"
     CDS             complement(1380..2189)
                     /locus_tag="VOX58_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HES5878509.1"
                     /translation="MDSKALNKQNLSRSKKILLNISDNPKNPKFYKLSRQELERAKIF
                     KTENLIKSQKYQRYKRGTIVFIHFGVNIGNEFSDSHFGIVLNKKDHPNNGKLTILPLT
                     SKNSKESLSINKEIFTSIMDDAEKTVQTIQNVLNLTTEVERIHHSLPVPPAFFQIKKE
                     NKYHDIWMKYYNRHDPKGIHVPVANITVRKWIQSDLDKINFLKKRYANYDKVSYAKLD
                     SITSVSKLKIAKPINDLDPVGKITLSKEIMDNIDKALARQLLSGQWGKLDN"
     gene            complement(2202..2945)
                     /locus_tag="VOX58_001283"
     CDS             complement(2202..2945)
                     /locus_tag="VOX58_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5878510.1"
                     /translation="MNILGNSIKEIRKSKKMTQKELAKLTGFKQNTISNHENGNRQLD
                     EVDIRKYAKALKIEPQQLFDYSSSPTNPQVELIPSTLQKINSTSSQLEHSRQIIVLDT
                     AETLLEQQKEIKNNEDTIAELFSYNYYDHAASAGTGQYLNDVQVEKIELPVDYDADFV
                     IPVYGDSMEPKYHSGDYVFVKLSVELTDGDIGVFEYYGDAYIKQLLINAEGAFLHSLN
                     QCGDYPDIPIDRDSDFRIIGEVMGSYRER"
     gene            3303..3452
                     /locus_tag="VOX58_001284"
     CDS             3303..3452
                     /locus_tag="VOX58_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878511.1"
                     /translation="MRERNEKFIGVLKDMHMQILRDFTNEEDPLTRCELAKGFLEIGD
                     YLSKQ"
     gene            complement(3438..3653)
                     /locus_tag="VOX58_001285"
     CDS             complement(3438..3653)
                     /locus_tag="VOX58_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878512.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSVEAIKKHNLPF
                     VEAEKQEQINNGKVIAEALERGESLFG"
     gene            3712..3870
                     /locus_tag="VOX58_001286"
     CDS             3712..3870
                     /locus_tag="VOX58_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878513.1"
                     /translation="MNWKKLMFGDLEYTFASRDGKEKTSIEFEGGLLPALLVLGGITW
                     LAAWFITK"
     gene            complement(3900..4499)
                     /locus_tag="VOX58_001287"
     CDS             complement(3900..4499)
                     /locus_tag="VOX58_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878514.1"
                     /translation="MDLLKNTNFLIPLISAIVSVSSIFISNWLGYRSQIRKLKFDEEK
                     EIYLTLYVPLIKWMNSQSFNNKSYYWLVAFPRYTTNAQDFLTGLLLKNFEKLPVSVAM
                     RYSEYTLNSATSLHFYRNTEYDYDYETFAKKASELFDLIIEQLLTEGTILSQKLSLPN
                     LSKSTLENFLADKKNYIGPRFLSLETHNKPLRPERPLPF"
     gene            4553..4762
                     /locus_tag="VOX58_001288"
     CDS             4553..4762
                     /locus_tag="VOX58_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878515.1"
                     /translation="MRPKKYPYSQKNYPAPKLHNVIETDNQFLIDDKRIHYVIEDSVK
                     TKTLGDGYVEVTLSIIAKSFTKSQG"
     gene            complement(4751..5137)
                     /locus_tag="VOX58_001289"
     CDS             complement(4751..5137)
                     /locus_tag="VOX58_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878516.1"
                     /translation="MFDYFKLYIIVLETFVETKPDDAYSLFDNLSLNSEFVKFSKDLD
                     KNIVISGTLEVIDNLLDDCLIKGKRRPTKDITFYFFDGVTTTGYLYLQSLKDNNFSSR
                     LKNVLKEEGIPLTPTSITRTIAKLTL"
     gene            5211..5438
                     /locus_tag="VOX58_001290"
     CDS             5211..5438
                     /locus_tag="VOX58_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HES5878517.1"
                     /translation="MTKKQRLKQEHLKPKKRLREERLKREYTKMYMADLIGLKNRKGY
                     SEKEDGLQPFKDYEMAIISQKFNVSESELFF"
     gene            complement(5546..6055)
                     /locus_tag="VOX58_001291"
     CDS             complement(5546..6055)
                     /locus_tag="VOX58_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878518.1"
                     /translation="MELFNTIIGVIALIVAVIALVHSIYYNMVKIKLSDCYISRVDKG
                     YDWMYDFSISNLSNVSVIIKKIELYNKDGKLISDNGFNPFQKYEADMQNEADDYYGLS
                     MPNYYMPLDYQWESSPFKSDTEVYPSSRENFSYYLDEKPVKIKITTDKRIHQFRKYQL
                     FFPHFDNNS"
     gene            complement(6314..6523)
                     /locus_tag="VOX58_001292"
     CDS             complement(6314..6523)
                     /locus_tag="VOX58_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878519.1"
                     /translation="MNLEELTPLLNSIDDFETVILHSLVGDFVIDHWIEPNRKNETLI
                     FMHNDQTTELKLSAILGTSTIPKSL"
     gene            complement(6673..6912)
                     /locus_tag="VOX58_001293"
     CDS             complement(6673..6912)
                     /locus_tag="VOX58_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878520.1"
                     /translation="MDTKEFMKVVSKHINQNFNVDNQLVEFVVAELNQMNAPITQKQA
                     QHIVNILEYVSKSTSKSTIAAMTNALLELGVLKGD"
     gene            7079..7264
                     /locus_tag="VOX58_001294"
     CDS             7079..7264
                     /locus_tag="VOX58_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5878521.1"
                     /translation="MWGKIEALLIEKKMTKYELSQKAGLNQNCLIDLKKGRKKSLKFD
                     DVVKIADVLGVSLDEFR"
     gene            7343..7639
                     /locus_tag="VOX58_001295"
     CDS             7343..7639
                     /locus_tag="VOX58_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878522.1"
                     /translation="MAIEIFGPEFRKKLLEDLIALNMEAIKIAQTKNAKSIEWITMKR
                     LEKETGWGRTKLTQWREQGKFNFKRSSENGKVLYDLADVNRFLRTSGYEKGETT"
     gene            7636..7773
                     /locus_tag="VOX58_001296"
     CDS             7636..7773
                     /locus_tag="VOX58_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878523.1"
                     /translation="MKLLDFIFAKPKKQEPIKHDTLRASSEKQWAEFDAYMRKQYGRQ
                     A"
     gene            7854..8183
                     /locus_tag="VOX58_001297"
     CDS             7854..8183
                     /locus_tag="VOX58_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878524.1"
                     /translation="MKTKIEIMRDFFKENPESTQRQASEKLGFTENTVKQYIWRDAKR
                     GYCIKDEEGRVTYLNIEDELSLINEWKSEIRRELIEQLLSANRHETSSEQIRMNAKTI
                     NQILGEI"
     gene            8183..8377
                     /locus_tag="VOX58_001298"
     CDS             8183..8377
                     /locus_tag="VOX58_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878525.1"
                     /translation="MEGMKKYINGQIKLIDTIEECVKEKWPEFDDKKVHKISLAIYQG
                     WSLTDINSTLEAIEMDLDRK"
     gene            8374..8658
                     /locus_tag="VOX58_001299"
     CDS             8374..8658
                     /locus_tag="VOX58_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878526.1"
                     /translation="MRIYVNKKGKPSVEFEFEDRRGGMFDTRLMLKESPLKEEFKADM
                     YKAIDDVLKKYEDIFDTPIFKELIIGKEEDIRKMIDYDDKFTELFGGIRH"
     gene            8655..9338
                     /locus_tag="VOX58_001300"
     CDS             8655..9338
                     /locus_tag="VOX58_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HES5878527.1"
                     /translation="MKITKATEITKTHNWRILIYGKPGNGKTYLTNYLKGKTLILDMD
                     HSSKTIAGNENIDIIQFDRTHPSDFMTEFLTELPELIKEYDNLVIDNITSWQSDWFIE
                     QGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPTNIFVTAWESTQDLTLESGQIITQ
                     YVPDIRKQVLSQTLGLTDVVGRIQVNPKTGGHGILLQGSDGLYAKNRLDNRTVCKAEE
                     LFNFEGSDA"
     gene            9374..10777
                     /locus_tag="VOX58_001301"
     CDS             9374..10777
                     /locus_tag="VOX58_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HES5878528.1"
                     /translation="MNEARRHILKHNVMIVSPPGSGKSVVISDIAKSATQKNGHVLFL
                     VHRKELIDQITNSFKFHGIDMNKVDLMTVGKAKNRLDKLTKPTLIITDEGHHGKASTY
                     QIIYEYFSDVPRIGFTATPWRLSGDGFTDTYDVMVLGKTVEWLINNNKLAPYDYYSVL
                     SIDTAKLKVQNGDYSNKSIDESFGKKIFGDVVQEYIKKANGQKAILYAHSVEASQAFA
                     KEFQSMGINAIHADAKTPKAKRDKIMKDFRDGKIQVICNVDLISEGFDVPDCTVTILC
                     RPTKSLVLFLQQSMRSMRYQPNKKAIILDHVGNWNIHGLPDTPHHWENYFRGGWKKKS
                     NKTNTVHAKECSVCSALWPLSQQLCELCNHDFGLKEKQEKERIEAELELIKRERFRIK
                     QLANKKFGKDLKTNWEIAQARVKDAGKGKPLYKLIYFYLKTDWVETNVNELAEVTGKS
                     EKEIYSAYNWLKKKLRG"
     gene            10782..11264
                     /locus_tag="VOX58_001302"
     CDS             10782..11264
                     /locus_tag="VOX58_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HES5878529.1"
                     /translation="MAGFTTDFSEVKEHAEFKEQPYEMIVYDAYEAVNERNGKKRVVI
                     DYVVRNDIKQEMQNYHLWDEQYPNSQTGKYHIGILMGKAKALGIKEGQHYDSFEAFLN
                     DFKGRTAKVTVKLDEYNGNKYPKVRYANQSDVPNSYHVWKEKATGFTQAEIEEDDLPF
                     "
     gene            11282..12835
                     /locus_tag="VOX58_001303"
     CDS             11282..12835
                     /locus_tag="VOX58_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HES5878530.1"
                     /translation="MTSNEFIEALSKLTTETDWGDPIFGESVLKAELRKHLFKIVPID
                     HNGYIHKLFYSEMVKDEDVMYFVSDGRKTYRFLFGDTILKTDKQGNEYLTYSVENNFP
                     PFAKLVIDYILGAYTFFENKLYDIRYKQFKLIDDFTLQTKYGFKDSGHILEILQGIHK
                     TLNIQPINYIEPYQIACKDFIIDLENSEIINQPPLQNVSYFKYYEVDYKTAINSKSIA
                     EEYLEYVIADSNSLNNAILQSYFIAQVACGVRPKTNFFISKSGVRTGKGLRHIALSGL
                     FNKIDVELDTLKSNGFEALQAWAMFSGGEMALATEQGDIQGNAMERVLKIIATEKTHV
                     ARAIGQNQSMVNLTSVLCIDTNRTVALSDEMNGRKVLIQFKDRPKTETDYERESIFRK
                     YWLAFTDCDKNPKIDGCIGFLLNSLERFQKIGKWYQWKDVEVFNDIDLDEFQVALINA
                     LQEVDFVQRTDNKEVIDLSLQVYGKSNHALSKAISEIGVRSRSKKVNGKTVRGYEIEN
                     KTRFDKYIL"
BASE COUNT         4499 a         2064 c         2421 g         3982 t
ORIGIN      
        1 acttggatgt tgttaaatta acgtttgttg agaattagtt gtcaatctag ttgcttgtta
       61 gtaacaaatt gattgaattt agttccaatg tcatcaattg ccgattgcgt gatgtgagta
      121 tatacgttca tagttgtctg taaatctcca tgccctaacc tatgttgcac ctgtttcaag
      181 gatagacctg attcgaaaag taagcttgca tgagtgtgcc tgaaaccatg aattgtgatt
      241 tgtggtaagt ctgtcccttt gataatttgc aatagccatt ttcgtggtaa acttggcgtc
      301 ataattccac ctgattcaga ttggaacata agagtagaag taggaaaagt ttcatgaagt
      361 tcttgtaaaa tttccaaagt ttcatcatct aagctgatta atctatcgct cgattttgtc
      421 ttcgtaacat ctatttctag tccaacttga gtacgtgtga cagctttatt aattgataga
      481 gtattaccat tcaaatcatc ccaagttagt gccagaagct ccccttttcg tattccagta
      541 aatgctaata atctaaacaa ggctattttt tcaatgttgt cagttttttc aactagttca
      601 aggaattgtt taagttgatc aagtgtataa aaattattat tcatttttct tttccttttt
      661 atttttggag gtgtaattgg caaagctgga ttgttattga tatatccgta tctaacagct
      721 agatttaaga tattcctaac aagcccaaga atttttcctc catattttaa atcgttgcac
      781 cattcttcag taagccgctg gataagtagt gggttgatgt ctccaatctt cactttatca
      841 agttttggca aaatatgttt attaatattt ctttctgttt ttaagtaggt actgccctgg
      901 acagtttttt catattcgtt tagccattgt ttagttaaca caccaaacgt catacttgat
      961 ttaggtttat caagttcagc ttgcaaacta attaaggttt cccttgcagc ctgcttagtt
     1021 ttgaaacctt ctcgcctaat atattttctc tttccattaa cataaccggc atacaaaaga
     1081 aatttataag cagtttcacc attttccttt ttatatgatt taattttcat tgcataccat
     1141 ctttcaattt gataaaatag gtacaagaaa atgacctttt taatggttgt ttcttatact
     1201 gtcttgcctc acgctcagag tcgccaaact ttgagagcgt ggggctcttt tattttttta
     1261 aaacaaaaaa gagcgcttat tcgaaaacag gcgctcacag tggagttgga cactccaaaa
     1321 taatgtttca gtggtagcga ggctaccaag attacacctt aattctaaca tagtgatatt
     1381 taattgtcaa gtttccccca ctggccagat aataactgcc ttgcgagagc tttatcaatg
     1441 ttgtccatga tttctttaga caatgttatt tttccgacag ggtcaagatc gttaattggt
     1501 ttagcgattt ttagtttact gacagaagtg attgagtcca gcttggcgta ggacaccttg
     1561 tcataatttg catatctttt tttgaggaag ttaattttgt ctaagtctga ttgtatccat
     1621 tttcggacgg taatgtttgc tacgggcaca tgaatccctt tagggtcatg cctattgtaa
     1681 tatttcatcc atatatcatg atatttattt tcttttttaa tctgaaaaaa cgctggggga
     1741 actggtaacg agtggtgtat ccgttctact tcagttgtta ggtttagcac attttgaatt
     1801 gtttgaactg ttttttcggc atcatccatt atgctcgtaa aaatctcttt gttaattgac
     1861 agactctctt tagagttttt tgatgttagc ggtaagatag ttagctttcc gttattggga
     1921 tgatcttttt tattcagcac aatcccaaag tgcgaatcgg aaaattcatt gccgatattt
     1981 actccaaaat gaataaagac gatagttcct cgtttgtatc tctgatattt ttgagattta
     2041 attaagtttt ctgttttaaa aattttagcg cgctcgagtt cttgtcttga taatttgtaa
     2101 aatttgggat ttttcggatt atcagagata ttcaacagaa ttttcttgct acggcttaaa
     2161 ttttgtttat tcaatgcttt actatccatt tctgctcctg cctatctctc cctataactc
     2221 cccataactt caccaataat cctaaagtcg ctatctctat ctatcggtat atctggataa
     2281 tcaccgcatt gatttaaact gtgtaaaaac gccccttcag cgtttataag caactgtttg
     2341 atataagcgt caccatagta ttcaaagacg cctatatcgc catctgtaag ctctacggat
     2401 agcttaacaa atacataatc cccagagtga tacttcggtt ccatggaatc accataaaca
     2461 gggataacaa agtctgcgtc ataatcgact ggtaactcaa ttttttctac ttgtacatca
     2521 tttagatact gacctgtacc agctgaagct gcgtggtcgt agtagttgta agaaaataat
     2581 tcggcaatag tatcttcgtt gtttttaatt tctttctgtt gttccaataa agtctcagct
     2641 gtatctaaaa caattatctg tctactgtgt tctaattgag aagaagtaga gtttattttt
     2701 tgtagggtag atgggatgag ttcaacttgg gggtttgtgg gagaagagga atagtcaaaa
     2761 agctgttgtg gctctatttt cagtgcttta gcatattttc gaatatctac ttcgtcaagt
     2821 tgtctattac cattttcgtg attagaaatt gtattttgtt taaatccagt tagttttgct
     2881 aactcttttt gggtcatttt tttggatttt cgaatttctt tgatagaatt tccgagaata
     2941 ttcataatat ttttcctttc tctactataa atataacatc aagaaacaaa aaagtaaata
     3001 aaaaaatctc aaaaagagat aaaaaatagt tgacaaaata tctcgttttg agatatacta
     3061 taatcaagct taagggatta agcaaaaatg aaagtaggta cagctgatga agtctaggct
     3121 aaacaaaaag cctaaacaca aagaactaga agtcgaaatc aagattcttt ggtttaagct
     3181 taagattcac tactcaatag agtggtgagg ataattagag ggctaagaag ccctctcccc
     3241 ctaacggggt aagtttagtt tagcacattg gctgtatctc cgcaagaatg aaaggagagg
     3301 ttatgagaga acgaaatgaa aagttcatcg gagtgctaaa agatatgcat atgcaaattt
     3361 tgcgagactt cacaaacgaa gaagacccct taacaaggtg cgagcttgct aaagggttcc
     3421 tcgaaattgg tgactattta tccaaacaat gactcgccac gctcaagagc ttcggctata
     3481 actttaccat tattgatttg ttcctgcttt tcggcttcaa cgaaaggtaa attatgtttc
     3541 ttaatagctt ccacagagtc ttcgtaaagt ttgattttat cttcgataga caaagtaggg
     3601 ctagaagaag cgacaatagc gagagctaaa tcttttgaat tagaaatttt cattagctta
     3661 tcctcctttc cgctaggata agttgattat aacattttta ggaggcacaa aatgaattgg
     3721 aaaaaactaa tgtttggcga tttagaatac acgtttgcca gtcgtgacgg caaagaaaaa
     3781 acaagcatcg aatttgaagg tggcttattg ccagcgctgt tggtgttagg tggtatcact
     3841 tggttagcag cttggtttat tacaaaataa aaagcccaca aggggcggaa aggggggagt
     3901 taaaatggca gtggtctttc aggtcttagg ggtttgttat gggtttctag tgataaaaat
     3961 ctgggaccga tatagttttt cttgtccgcc aaaaagttct ctaaagtgga tttggataag
     4021 tttggtaagc ttagcttttg ggataatatt gtcccttctg ttagcaattg ttcaataatg
     4081 agatcaaata attcagatgc ttttttggca aatgtttcat aatcataatc gtattcggtg
     4141 ttacgataaa aatgtaaaga ggttgcagaa tttagggtgt attcagagta tctcatagca
     4201 actgatactg gtaatttttc aaaatttttt aacaacaagc ctgtcagaaa atcttgtgca
     4261 tttgttgtat atctagggaa cgccactagc caataataac ttttgttatt gaatgattgc
     4321 gaattcatcc attttattaa cggtacatag agggttaggt atatttcttt ttcttcatca
     4381 aattttaatt tcctgatttg acttctataa ccaagccaat tgcttataaa aatagaagaa
     4441 acagagacta ttgctgaaat taacggaatt aaaaaattgg tatttttcaa taaatccatg
     4501 attttctcca atcattttat ttaaattata ccacagaaag ggggtggaat agatgcgccc
     4561 caaaaaatat ccgtatagcc aaaaaaatta tccagcgccg aagcttcata acgtcattga
     4621 aaccgataat cagttcttaa tagatgataa aaggattcat tatgttattg aagattctgt
     4681 aaagacaaaa acccttggcg atggctatgt tgaagtaaca ctttccataa ttgccaagag
     4741 ttttacaaaa tcacaaggtt agtttggcga tagtccttgt gatagaagtt ggtgtaagag
     4801 ggataccttc ttctttaagg acattcttta aacggctcga aaaattatta tctttaagac
     4861 tttgaagata taagtaacca gttgttgtta cgccatcgaa aaaatagaaa gtaatatctt
     4921 ttgttgggcg acgttttcct tttatcaagc aatcatcaag taaattatca attacttcta
     4981 aagtgcctga tattacaatg tttttatcta agtctttaga gaattttaca aattcactgt
     5041 taagacttag attatcaaat aagctataag cgtcgtctgg tttggtttcc acgaaggttt
     5101 ccaagacaat tatataaagt ttgaaatagt caaacatcac attcacctcc tttctgccca
     5161 tattatagca gattggagga actaaaaaca gatagaaagg aggtggggga atgacgaaaa
     5221 aacaacgttt aaaacaggaa catctcaaac ctaaaaagcg tttaagagaa gaacgtttaa
     5281 aacgtgagta cacaaaaatg tatatggctg atttaattgg tttaaaaaat agaaaaggtt
     5341 actctgagaa agaagatggt ttacagcctt ttaaagatta tgaaatggct atcatttctc
     5401 aaaaatttaa tgtttcagag agtgaattat ttttttaaac aaaaatatct caaaacgaga
     5461 taaagtgtac aacactagaa aggataaaag aaaaatgctc ctaacggcag cacttatctt
     5521 aattttatta acgaatatcg ctatcttaac tattattgtc aaaatgggga aagaataact
     5581 gatattttcg gaattgatga atacgcttat cagttgtgat tttgatttta actggctttt
     5641 catctaaata gtaagagaaa ttttctcgac tagatggata tacttcagta tctgacttaa
     5701 atggcgatga ctcccattgg taatctaatg gcatataata attcggcatt gacaatccat
     5761 aataatcatc agcttcattc tgcatgtcag cttcgtattt ttgaaaggga ttgaagccat
     5821 tatcgcttat tagttttcca tctttgttgt aaagttcaat ttttttaata atgactgaaa
     5881 cattcgataa gttactgata ctaaaatcat acatccaatc gtaaccttta tctactcttg
     5941 aaatgtaaca atcagataat tttatcttaa ccatgttgta gtagatagag tgaacaagag
     6001 caatcacagc aacaattagc gcaatgacgc cgataattgt attaaataat tccattttta
     6061 ttcttccaat cgtttttatt ttaattatac caaaatagaa aggacacata tgaacgaaat
     6121 agaaaacaaa ctagaagaac ttgaagaaat ggtaataaac atggatgaag tagatgtagt
     6181 gattccatgg aagatagcaa aaaacctgct acaaagagca ggttacttat cagaagagga
     6241 acatcgccta ctaagttgga gactcggaaa atcaaaatat gcaggcaaac gttccgaaaa
     6301 agtccgcaat ttgctagagg gacttaggaa tagtggaagt tccgagaata gctgataatt
     6361 ttaattctgt tgtttggtca ttgtgcatga aaatgagagt ttcatttttg cgattaggct
     6421 caatccaatg atcaataaca aagtctccga caagactatg taaaataaca gtttcaaaat
     6481 catcaatact attcaataga ggagttaatt cttctaaatt catttagtta ccctccttcc
     6541 taataggata actacattat acaacagaaa ggaagcacat gagaccaaaa cgatatccgt
     6601 atagcggaga gaaaaagaga gcctatcata agatagaccc tgaactgata aaaaccggta
     6661 aagttgattg catcaatctc ccttaagtac gccaagctct aacaatgcat ttgtcatagc
     6721 tgcgatagta gatttagagg ttgacttaga gacatactcc aaaatattaa cgatatgctg
     6781 agcttgcttt tgtgtaatag gtgcattcat ttgattaagt tccgcaacga caaattcaac
     6841 taattggttg tcaacattga aattttggtt gatatgtttt gagacaactt tcataaattc
     6901 ttttgtatcc ataattaatc tcctttcttg cttatttgac taaacaagat tagaagagta
     6961 gaggtctctc gagagatctt gttgaatcat acgaatatta tatcaagact atattgaaaa
     7021 atcaatatat tgtatataaa aagttatcaa caactacatg ttgtgcttag gaggaattat
     7081 gtggggtaaa atcgaagctc tattaattga gaaaaaaatg accaaatatg aactgtcaca
     7141 aaaggctgga ttgaaccaaa attgcttgat tgatctaaag aaaggaagaa aaaaatcatt
     7201 gaaatttgat gatgtcgtta aaattgccga tgtcttaggt gtcagcttag acgaatttag
     7261 ataacaaaaa aagtccgacg gcaatcggac tcaaaacaaa ttttaattta cttaattata
     7321 acataagaga gagggaaaaa ctatggccat tgaaatattt ggacctgagt ttagaaaaaa
     7381 actgcttgaa gatttaatcg ctctaaatat ggaagcgata aaaatagcgc agaccaaaaa
     7441 cgccaagtct attgaatgga taaccatgaa gcggctagaa aaagaaactg gatgggggcg
     7501 aactaaattg acccagtgga gagaacaagg gaaatttaac tttaaaaggt catcagaaaa
     7561 cgggaaagta ctatatgacc tagcagatgt taatagattt ttacggacca gtggatatga
     7621 aaaaggagaa acaacatgaa attattagat tttatttttg caaaaccaaa aaaacaggaa
     7681 ccgattaagc acgacacgct tagagcatca tctgaaaagc agtgggccga atttgatgcc
     7741 tatatgagga aacagtatgg acgacaagct taatcagata ttgatagcca tgcgaaacta
     7801 ccgcacaaat ggtgatgata gcaagtattt agaggatatg gaggtcattt taaatgaaaa
     7861 caaaaattga aatcatgcgt gattttttta aagaaaatcc tgaatcaact caacggcaag
     7921 cctcagaaaa gcttggattt acggaaaata cagttaaaca atatatttgg agagatgcta
     7981 agcgtggcta ctgtattaaa gacgaagaag gtcgtgtaac ttatctaaac attgaagatg
     8041 aattatcgct tattaatgaa tggaaaagtg aaattagaag agaattgatt gaacagttac
     8101 tatcagctaa tcgacatgaa acgtcaagcg aacaaattcg tatgaacgct aaaacaatca
     8161 atcagatttt aggagaaatt taatggaagg tatgaaaaag tatatcaatg gtcagattaa
     8221 actaattgat actattgaag aatgtgtcaa agaaaaatgg cctgaatttg atgataaaaa
     8281 agtacataag atatctttag ctatttacca aggatggtcc cttacagata ttaatagcac
     8341 actcgaagcg atagaaatgg acttggatag aaaatgagaa tttatgtaaa caaaaaaggt
     8401 aaaccatctg ttgaatttga gtttgaagat cgacgcggtg gcatgtttga tacaagatta
     8461 atgttaaaag aatcaccact caaagaagaa tttaaagccg atatgtacaa ggcaattgat
     8521 gatgttttaa aaaaatacga agacattttt gatactccta tttttaaaga attaataatt
     8581 gggaaagaag aagatattag aaaaatgatt gattacgatg ataaatttac cgaacttttt
     8641 ggagggatta gacattgaag attactaaag cgacagaaat tacaaaaacc cataattggc
     8701 gcatattaat ctatggtaaa cccggaaacg ggaaaactta cttaactaat tatttaaaag
     8761 gcaagacact gattttagac atggaccatt cgtctaaaac gattgccgga aacgaaaaca
     8821 ttgatattat ccaatttgac aggacgcacc ctagcgactt tatgaccgaa ttcttgacag
     8881 aactaccaga acttatcaaa gaatatgaca atttagtcat tgataatata acaagttggc
     8941 aatcagactg gtttatcgaa caaggtcgta aatccaaaaa tggaatcaca aacgaattgc
     9001 aacaatacaa catgtggacc aattactatt tacgagtatt gactaccatt tacagccaac
     9061 ctactaatat ttttgtgact gcttgggaat caacgcaaga tttaacgctc gagagcggac
     9121 aaattatcac gcaatacgtg ccagatatcc gcaaacaagt cctaagccaa acgctaggtt
     9181 taaccgatgt tgtcggacgt attcaagtaa atccaaaaac gggcgggcat ggaattttgt
     9241 tgcagggcag cgacggacta tatgcaaaaa ataggcttga taataggacc gtttgtaagg
     9301 cggaagagtt gtttaatttc gaagggagtg atgcgtaacg gtataccatt tacacgaata
     9361 tcaaacagaa cttataaatg aggcaagaag acatatttta aagcataatg tgatgattgt
     9421 tagtccacct ggaagtggta agtcagtagt catttctgat attgctaagt cagcgactca
     9481 aaaaaacgga cacgttttat tcttggtcca tcgcaaagaa ttaattgacc aaatcactaa
     9541 tagttttaaa ttccatggaa ttgacatgaa taaagtggat ttaatgacag ttggtaaagc
     9601 taaaaaccgc ttggataaat taacaaaacc aaccttaatt atcacagatg agggtcatca
     9661 tgggaaagct agtacctacc aaataatcta tgaatacttt tcagacgtgc cgaggattgg
     9721 ttttactgcc acaccttggc gtctatcagg cgacggtttt acagacactt acgatgttat
     9781 ggttcttggt aaaacggtcg agtggcttat caataataat aaacttgcac catatgatta
     9841 ttacagtgtt ctatcaattg atactgcgaa attaaaagta caaaacggag attactccaa
     9901 taaatcgatt gacgaatcat ttggtaaaaa gatttttggt gatgtagttc aagaatatat
     9961 aaaaaaagcg aacggtcaga aagctatttt atacgctcac tcggtagaag catcgcaggc
    10021 atttgctaaa gaattccaat ctatgggaat taatgcaata cacgcagatg caaaaacgcc
    10081 taaagccaaa cgggataaaa tcatgaaaga tttccgtgac ggtaagatac aagttatctg
    10141 taacgttgat ttgatatcag aaggtttcga tgtcccagat tgtacagtaa ctattctttg
    10201 tagacctaca aaatcattag tattattttt gcaacaatct atgcggtcga tgagatatca
    10261 acctaataaa aaagccatca ttttagatca cgtaggaaat tggaatattc acggcttacc
    10321 tgacacaccg catcattggg agaattattt ccgaggaggg tggaagaaga agtcgaataa
    10381 aactaacacg gtacacgcaa aagaatgttc tgtgtgttcg gctttgtggc cacttagtca
    10441 acagctctgt gaattatgca atcatgattt tggattgaaa gaaaaacaag agaaagaacg
    10501 catagaggca gaacttgaac tcataaaacg tgagcggttt agaatcaaac aacttgctaa
    10561 taagaagttt ggtaaagatt taaaaacaaa ctgggaaatt gcccaagcta gagttaaaga
    10621 cgctggtaaa ggaaaaccat tatataaact tatctatttc tacttaaaaa ctgattgggt
    10681 agaaacaaac gttaatgaac ttgccgaagt aacaggcaag tcagaaaaag aaatatatag
    10741 cgcctacaat tggctaaaaa agaaattaag aggataaaaa catggcagga tttacaacag
    10801 atttttcaga agttaaagaa cacgcagaat tcaaagaaca accatacgaa atgattgtct
    10861 atgatgcata tgaagcagta aatgaacgta acggcaaaaa acgtgttgtt attgactacg
    10921 ttgttcgaaa cgatatcaag caagaaatgc aaaactacca cttatgggat gagcaatatc
    10981 ccaactcaca aactgggaag tatcatatcg gcatcttaat gggtaaagca aaagcacttg
    11041 gtatcaaaga ggggcaacac tacgatagtt ttgaagcgtt tttaaacgac ttcaaaggac
    11101 gcactgcaaa agtaaccgtt aaacttgacg aatataacgg aaataaatac ccgaaagttc
    11161 gttacgcaaa tcaaagcgat gtgcctaaca gctaccacgt atggaaagaa aaagcaactg
    11221 gatttacaca agcggagatt gaagaagacg atctaccgtt ctaatttaga ctgggggata
    11281 aatgacatca aatgaattta ttgaagcttt atcaaaattg acaaccgaaa ccgattgggg
    11341 agacccaatc ttcggcgagt cagtgcttaa agctgaatta cgaaaacact tatttaaaat
    11401 tgtcccaatt gatcacaatg ggtatatcca caaactgttt tattcagaaa tggttaaaga
    11461 tgaagatgtc atgtattttg tgtcagatgg acgaaaaact tatcgctttt tatttggaga
    11521 tacaattcta aagactgata aacaaggcaa tgaatatctc acatactccg ttgaaaataa
    11581 ttttccacca tttgcaaaac tagttatcga ctacattcta ggtgcttaca cgttttttga
    11641 gaataaactt tatgacattc gatataagca atttaaattg attgatgatt ttacacttca
    11701 gactaagtat ggtttcaaag actctggtca cattttagag atactacaag gtatccacaa
    11761 aacattaaac atccaaccaa tcaattatat tgaaccatat cagattgctt gcaaggattt
    11821 cataatcgac cttgaaaatt ctgaaatcat taaccaaccg cctttgcaaa acgtatctta
    11881 ttttaagtat tacgaagtag actacaaaac agcaataaac agtaagtcta ttgcagaaga
    11941 atatcttgag tacgttattg cagatagcaa ttcgttaaac aatgcaatac tacaatctta
    12001 ttttatcgcc caagtagcgt gtggtgttag acctaaaacc aacttcttca tctcaaaatc
    12061 tggagtaagg actggtaaag ggttaaggca tatagcttta tctggtctat tcaataagat
    12121 tgatgttgag ctagatacat taaaaagtaa tggatttgaa gcgttacagg cgtgggcgat
    12181 gttctcgggt ggagaaatgg ctctagcgac ggaacaggga gatattcaag gcaatgcaat
    12241 ggagcgtgta cttaaaatta ttgcaacaga gaaaacacac gttgcacgag caattgggca
    12301 aaatcagtca atggttaatt taacgagtgt tctgtgtatt gacaccaacc gtactgttgc
    12361 gctgtcggat gaaatgaatg gacgaaaggt tttaattcag tttaaagata gaccaaaaac
    12421 tgaaactgat tatgaaagag agagcatatt caggaaatat tggctagcat ttactgactg
    12481 tgataaaaat ccaaaaatcg atggatgtat cggttttctg ctaaattcac tcgagcgttt
    12541 ccaaaaaatt ggtaaatggt atcaatggaa agatgttgaa gtatttaatg atatcgattt
    12601 agacgaattt caagttgctt taataaatgc attacaagaa gttgattttg tacagcgaac
    12661 tgataacaaa gaagttattg acttatcatt acaagtttat ggaaaaagca atcatgcatt
    12721 aagtaaagcc atatctgaaa ttggtgtacg tagcaggtcg aagaaagtta acgggaaaac
    12781 agtcagaggg tatgaaatcg aaaataaaac acgttttgat aaatatatcc tttaaaaagg
    12841 taacgcatgg tcacgcaaag ttacgcagaa ttttccatgt tgcgtaacct actcaatctc
    12901 tttagtacca atcgatttca gcatttattt aagaaaggtt acgcagtaac aaacaataag
    12961 tctaat
//
LOCUS       DAUVUY010000055        12565 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.055, whole genome shotgun sequence.
ACCESSION   DAUVUY010000055 DAUVUY010000000
VERSION     DAUVUY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.055"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..1442)
                     /locus_tag="VOX58_001304"
     CDS             complement(<1..1442)
                     /locus_tag="VOX58_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HES5878531.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVMVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGNWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVV"
     gene            complement(1620..2351)
                     /gene="aphA"
                     /locus_tag="VOX58_001305"
     CDS             complement(1620..2351)
                     /gene="aphA"
                     /locus_tag="VOX58_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HES5878532.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            2587..3525
                     /locus_tag="VOX58_001306"
     CDS             2587..3525
                     /locus_tag="VOX58_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HES5878533.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTYRPVGKNHSICGTDLHIL
                     GGDVPACKEGTILGPEGIGIVKEIGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSSH
                     CEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVLP
                     SHVKPGDNVCIVGAGPVGLAALLTVQLFSPANIIMVDLSQNRLEAAKTFGATHTICSG
                     SSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFNL
                     DELWIKNITLNTGLVNANTTEMLLNVLKVIIDNDIS"
     gene            complement(3607..4773)
                     /locus_tag="VOX58_001307"
     CDS             complement(3607..4773)
                     /locus_tag="VOX58_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HES5878534.1"
                     /translation="MKNQLGQLALEQAKTFGGKLEVQPKVDITNKHDLSIAYTPGVAG
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLQKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEADQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPGDALSTTNIIPDAFKEGVAEIVAKSVRSVVLKS"
     gene            complement(4805..6136)
                     /locus_tag="VOX58_001308"
     CDS             complement(4805..6136)
                     /locus_tag="VOX58_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HES5878535.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLPVYLVLATLILITG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHPSYSGQGQLVKIGHSEDMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKEVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMNPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            6297..7838
                     /locus_tag="VOX58_001309"
     CDS             6297..7838
                     /locus_tag="VOX58_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HES5878536.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTRPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLFLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKTGILQLCNQAGQELIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSVTKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGWQH
                     ERFNH"
     gene            7819..8484
                     /locus_tag="VOX58_001310"
     CDS             7819..8484
                     /locus_tag="VOX58_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HES5878537.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIIENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(8539..9612)
                     /locus_tag="VOX58_001311"
     CDS             complement(8539..9612)
                     /locus_tag="VOX58_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HES5878538.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(9605..10381)
                     /locus_tag="VOX58_001312"
     CDS             complement(9605..10381)
                     /locus_tag="VOX58_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878539.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            complement(10378..11172)
                     /locus_tag="VOX58_001313"
     CDS             complement(10378..11172)
                     /locus_tag="VOX58_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878540.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            complement(11156..12310)
                     /locus_tag="VOX58_001314"
     CDS             complement(11156..12310)
                     /locus_tag="VOX58_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878541.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWVFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            complement(12356..>12565)
                     /gene="murB"
                     /locus_tag="VOX58_001315"
     CDS             complement(12356..>12565)
                     /gene="murB"
                     /locus_tag="VOX58_001315"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="catalyzes the reduction of UDP-N-acetylglucosamine
                     enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HES5878542.1"
                     /translation="AGQLIMEANLKGHRIGGVEVSEKHAGFMINVSDGTAKDYEDLIA
                     YVIETVENHSGVRLEPEVRIIGENL"
BASE COUNT         4059 a         2620 c         2194 g         3692 t
ORIGIN      
        1 acaacttgag atttttgatg gtgaatttgg gttgtaatta agacattttc tggaagcttt
       61 aactctttaa catatttccc tgcaatctta tcaccaacag tcaattcaat taatgttggt
      121 tcaacaagat tagttggatg cttcattgcc atcttatcta gcatagcttc gtaaattggc
      181 tgtccattta acaaatccat cactaaatag gagacaaaag taactacagc tatggccatt
      241 aagggtttca aatcacctac catttcagtt accaaaatca tgcctgtcag tggtgctttt
      301 gaaattgccg caaaataccc tgccatccct aatatcaaaa aaagagaaag aaaagattgg
      361 ttcaataaac ctaattgaga agctatttgc ccaaataata ggcctgccaa agcacctaaa
      421 gttaaaatgg gtaaaaaaat acctccaggt aagccacttc cataactaaa catactaaca
      481 ataaatcgaa taataaaata aaagaagata gtcatcaaag gcaattgttg attggataaa
      541 gaaagtatta aaccatgccc tcctccaagt aactgtggta aataatagcc aatcggtaaa
      601 attagaaaaa ccataataat accgtaaaaa taagctggca gatgtaacca attccctaaa
      661 ataacataaa gtttattaaa attaaggata actatctcat ataaatagcc taagcagcct
      721 agaaataatc caagtaaaag caacaaccaa tattgattta gccctaaaaa aggcatcgcc
      781 tctgacattg ctaagactgg tttcaatcca aaaatattca gagaaataaa attagccact
      841 aagctagcca ctagcgctgt aatccaaatt aaacgcgaaa aatgatggta aatttcttca
      901 acaacaaaca agaggcctgc tattggagca ttaaaagctg ctgatagccc tgctgctgct
      961 ccgctggcaa ttagcactcg cttttccaaa cgactcgatt ttaaaaactt agctaatcct
     1021 ttggctgaca tagcacctaa ctgaatcgaa ggaccctcac gaccgagcat aaatcccata
     1081 gaaatagcca tgattcctcc taaaaatttt ttccaaagga cactccacca atctggggac
     1141 attagcccct ttagttctcc ttcgacatgg gggatccctg atccttttat atcagaatca
     1201 gactttacta aaaaacctac aaaaagaaca gctaacaaac tgacacttag tataggaagt
     1261 agcaacagcg aattgatatg agcgtatcgg taccattcaa tcacccagtc agctgtcact
     1321 tcaattaaca accggaataa actaacaata acaccagcaa taataccaac cataacacca
     1381 cgccaaacat aggcaataat tgatttgttg gaaaaggtga attcattttt atggttttcc
     1441 atgttatcct ctctttcttt taaatacact gttaccattg tatcaaagac aaccttaagt
     1501 taccagtgat tcttttttag aactgatttc tgtaacccaa aaaacccact gggctattgg
     1561 ttactcagtg gggtgatgaa agaaattact gttacttcct ttcttacaca aatatttgac
     1621 taataagccg aattttctaa aacctcttct ccgtatcctc cagcttttgg caaaggaaga
     1681 ttggttgaat taggagctct taaaatgcga ataggtcttg cacctgcctc tttggcagca
     1741 tttatatctt catcactatc tccgtaatga atttgtgagc cgtttttctt tatataatat
     1801 gttttatcat actgatacgg cttcacagct ttatctcctg tataattgat agctattggc
     1861 ttgtctaact taaaatcttt agccagtgac ttagctgttt tatcaatctc acctttttta
     1921 tacatcgatc ctcgagttcg gccagtaata aagacaattt tatctcctcg tttttgatgc
     1981 attgcgatta gttgctttgc atactctttt ggaatagaat cctgatctcc ccgtttagcg
     2041 actaagtccc aaaatttttg cttatgcaaa aaatcaaatg agcctggagt tatatattct
     2101 ttgccgtact gaaaatattg gctagtaaat aacaaagtat catcgatatc aaaactaacc
     2161 gttataggtt tttgtccttc taaactgctc gcgatatcgg caatagaaat caattttact
     2221 gttgcctgat ttgaaatggc cgttatacct tcttgtgtat aaggaacttt agggccacta
     2281 gcttcgacag gcaatgctat gattccacaa aaactaactg taaacaggat actggtaaat
     2341 tcttttttca tctaaaaacc tccttaatca ttattgtaca aaaatacacg gtttaagtaa
     2401 agcggtttca aattcttata atagtgccct ttaagttctg aataaggatg aagttattca
     2461 acaaagatga tctaaaaaat tatcataatc gatttaaaaa atattgaaaa ccctttctta
     2521 aactattata atatctgtgt aaaaatgaat tggttgatta cctctcaacc ttataaagag
     2581 ttaaatatga aagcagcaac ttacctttca accggaaatc tacagctcat agacaaacca
     2641 aaaccagtca ttataaagcc tacatatcgt ccagttggta aaaaccactc tatttgtgga
     2701 actgatttgc atatcttagg tggggatgtt cccgcatgta aagaaggaac aatccttggt
     2761 cctgaaggaa ttggaattgt taaggagatc ggtgatgcgg tcactaactt taaaatcgga
     2821 gataaagtga ttatttcttg cgtcacatca tgtcatacct gttattattg taaacgaggc
     2881 ctttcgtcac attgtgaaga tggcggttgg attcttggcc atttaattaa tggaacacaa
     2941 gcagaatatg tccatatccc acatgcagat ggtagtctct atcatgctcc tgatacgatt
     3001 gatgatgagg cactggtcat gctttctgat attttaccaa cttcatatga aattggtgtc
     3061 ttgccatcac atgtcaagcc tggagataat gtttgtattg ttggtgccgg tccagtagga
     3121 ctcgcagccc tactaaccgt tcaacttttc tctccagcca atattattat ggtcgactta
     3181 tctcaaaatc gccttgaagc agctaaaacg tttggagcca cacacacgat ttgttcagga
     3241 agtagtgaag aagtcaaggc tatcattgac gatataacca atggacgtgg tgtcgatatt
     3301 tccatggagt gtgttggtta tcccgcgaca tttgatattt gccaaaaaat tatatcagta
     3361 ggtgggcata ttgctaacgt tggggttcat gggaaaccag ttgatttcaa cctggatgaa
     3421 ttatggatta aaaatatcac cttaaacact ggacttgtta atgccaatac aactgaaatg
     3481 ctactaaatg tcttgaaagt tattattgat aatgatatta gctaatcaca aaataagtag
     3541 aagctagaaa tatgcttcta cttattttgt gatttaaagt ataactttct ctttttttca
     3601 tatattctat gatttaagga ctaccgacct gacgctttta gcgacaatct cagcaactcc
     3661 ttctttaaag gcatctggaa taatattagt agtgctcaaa gcgtcacctg gaacaagact
     3721 ggcaatacct tttgcggcag caatttgcat ctcaacagta atggtttttg cccgtgcatc
     3781 taatgctccc ctaaaaatac ctggaaaggc aaggacatta ttaatctggt taggaaaatc
     3841 actacggcca gtacctacaa tgtaagcccc cgcttcaagg gcttcatcag ggtaaatctc
     3901 tggaataggg ttggccatgg caaaaattac tgggcgcgct gccattttac taatccactc
     3961 tgcttttaag actcctggtg ctgatacgcc aatgaagata tctgctcctt caagagcatc
     4021 ttctaaggta cctgatttaa actcacgatt agtgactttc gctatatcca aatggtgtgg
     4081 tgctaactga tctgcctctt gttcattgat aatgccaaat ttatccacta ctgtcacttt
     4141 cgtagcacct gctgctaata atttacgggt aatggataat cccgcagatc caccaccgtt
     4201 caccacgatg gatacctcat caagggattt ttgaagaagt ttcaaactat taaagatggc
     4261 tgctaacacc acaatagctg ttccatgctg atcgtcatgg aaaacaggaa tatgacattc
     4321 cttaattaag cgttgctcaa tttcaaaaca gcggggcgca ctaatatcct ctaaattaat
     4381 accaccaaag gttggtgcaa gcgccttgac aatactaata atttcttcag tatctttggt
     4441 atctaataca atcggaatag catcgacacc tgcaaatgct ttaaaaaggg ctgcttttcc
     4501 ttccataact ggcatggctg cttcagggcc aatatccccc agaccaagaa ccgcagtacc
     4561 atcactgata acggctactg tatttttctt ggtggttaag tcatatgcta atgttttatc
     4621 ttttgcgatg gcagatgaca caccagctac acctggtgta taggcaatgc tcaaatcatg
     4681 tttatttgta atgtcaactt ttggttgaac ttccaattta cctccgaacg ttttagcttg
     4741 ttcaagggca agttgaccta attgattttt cataggaaat acctctcttt tttattggtt
     4801 tggattaact aaaaataatt cttaaaatag ctgtcatggt gataacggta atagccccac
     4861 ctagacgagt agcgacttgg gcaaacggca ttagattcat gcggtcagcc gtactcaaaa
     4921 tagccacgtc ccctgttcca cccataccac tttgacaagc tgaaataata gctgcttcaa
     4981 cagggttcat gttaaggaag cgagagacaa agaaaccgac actgacaacc gttaacacca
     5041 cactaatcac cacaataaaa tattgccagc taagggttgc cacaacctct tttaagggga
     5101 tataaagcag tccaagccct gccattaacg gaaaagtaaa gttgctggaa ataaatttat
     5161 aaagttgctt ggcaccattt tgggtttctt ggggaataac attcaggtat ttcaagaagg
     5221 ctgccagaat aatcatcaac accggtcctg gaaaacctgt taaatgctgt aacaagccac
     5281 cagcaataaa aagtgaacaa gctgttaaaa cgcctgcccc cattaattta acatctaggg
     5341 ctccagaatt gtcttttagg gcatcagaca tgtcttcgct gtgaccaatt ttaaccagtt
     5401 gcccttgacc agaataactt gggtgcttct ctccaaaacg attgagcaaa gccgtgcaca
     5461 agatagcaaa gaaattccca ataatggtgg ctggaatcag ttgagccact aattgctcac
     5521 ttccgactcc tgtaatggca ctgtaaccaa gagataaggg taaaatacct tcaccaattc
     5581 ctccagcaag gactggcgtc acaacataaa agagggtatg ttgccagtct aatcctaaaa
     5641 tgacgccaac caaagtccct acacccattg cacaaaccat tccaagtaac atgggaataa
     5701 tcatccgaaa aagaccttga atcaagattt ttcgattcat gcctaaaata ctaccgcaga
     5761 ccagacaggc aatataaaaa taaagaaaat tggcttgctt catcaagaca ttcgtcgctt
     5821 ctaagacatt ggggttgagg agattaaaaa aaaccaaaat cgaagggact aagagtgata
     5881 aaatagctgg gccaccaaaa tgtttgaggc ctggaatggt cgctccaatg gttcctagta
     5941 gccaaccaag ggttaaaata acagcaaagc cacccagcat attaacaggc aattgttgga
     6001 gccagcctgt gatcaaaatt aaggtagcta aaaccagata aacaggcaag ggaacggagc
     6061 ctattcgcct attttgccat gcttttgaat gctcagacaa gtctttttga ggcattttct
     6121 tgcttattgt ttccatgaaa caacctcctt tagtgataag actagtataa aagagaacat
     6181 gtcaaaagca tagtttattt aagttgttta actactttaa ttttttcaag ttatttaatt
     6241 aatttaagct agcaggtcat ttcttatcgt gttatactca ataaagagga gaccttatga
     6301 aaaaaccact acgtttatgg gccagtttat cgttgatttt ggtatctatg attgttgtca
     6361 caacctccct attttacggg attatgctac atgatacgca ccaatccatc aaaaatcagg
     6421 aaacccattt gctaacgtca actggaaaaa tgttagcaag tcatcaagca attaaagaat
     6481 tactcctaaa caaccaacca aatgctaaaa caacagccta taccaactcc attgcctcca
     6541 tctataacct tgattatgtg gtcgtgatga acatgaaagg gattcgactc acccgtccta
     6601 acccgaaaaa tatcggaaaa ccttttcaag gaggtgatga ggaggctgtt ctagcaggaa
     6661 aaaaggttat ctctaccgcc aagggcactt taggaaagtc acttcgctat ctagtacctg
     6721 tgtttgacgg tgacaaacag attggtgcta ttgcggttgg cattaaacta actaccctaa
     6781 atgatgtggc tttaacgtca aaacgcaatt ataccctgtc tttattcctt tgtcttctta
     6841 ttagtttact ggtcaccagt tttatttctt ttcgactcaa gcgtcaactg caccagttag
     6901 aaccaagtga aatttaccag ctctttgaag agcgtaatgc catgttggat caaatcgaag
     6961 ccgccgtatt tgtggtagat aaaactggta ttttacaatt atgcaatcag gctggacaag
     7021 agttaatcgc tcgaaaatgc cagttgggaa aacctactgg caacagtttc aatcacctct
     7081 ttcctgattt tccaaaactt agtttgcaag aggggcatga acaactcttt cggtatggtg
     7141 aggaagacta cttgttagcc atttcaccca tctgtgtcaa aaacgatcac cgaggccaca
     7201 ttattttcat gcgagaagca gttaaggcta ttgatacctt ggatcagcta gcctatacca
     7261 ccgcctatgc ttctgctcta caagcccaaa ctcataaatt catgaatcaa ttgcatgtta
     7321 tttatggctt agtagatatt gcttattatg accaattgaa aatttattta gacagtattt
     7381 tggagcccga aaatgaaatt ctcactagct tatctgtcct tgttaaggag cctcttctag
     7441 ctagttttct cattggagag caagagaaat accaagaatt aaatgtccat ctcaagattg
     7501 atgttttaag cgagattcct cattctgtta ccaagaatca acttaataat ggtttgatga
     7561 tttaccgctt tatccatact aatcttctaa ccacactgag acccaaatca ctcgttttga
     7621 gtattcaaca tgatcagaat catttaatca gccattacac cttgacagac aattggattg
     7681 atttggagcg ggtacagcct atttttgact taccttattt tcaacagctc ttgaccgata
     7741 caaattccca attccatcaa catcaaaccg taaccgagtt acagttctca gtgacaacac
     7801 cttatgtagg atggcaacat gaacgtttta atcattgaag atgatcctat ggtggatttt
     7861 atccatcgca attacctcga aaaactaaat ctttttgatc gcattatatc cagcgactct
     7921 atgaaagctg ttcaaagcat acttactgat tacgcaattg acctcatttt actggatatt
     7981 catattacag atggcaatgg gattcaattt ttggagaaat ggcgtgctca gcatattcct
     8041 tgcgaagtca ttattatttc agccgcaaat gatgggaata ttatccgaga tggttttcat
     8101 ttaggaatta tcgactacct catcaagccc tttacctttg aacgctttca agaaagcatc
     8161 caacagtttg ttacgcatcg agaacatttg gccaatcagc aactagagca ggcccaaatt
     8221 gaccaactga aatgtttgac tagcaaaaaa gacactaaaa acaaacaatt gttagaaaaa
     8281 ggcttatctg aatccacttt ccagtggatt atagaaaata tcaaagcctt tgatcaacct
     8341 tttactatcc aagaattagc ttccgcttgt cacttatccc atgtctctgt ccgtaaatac
     8401 attgcttatt tagaagaaaa taagcaactg aacagccaac aaatttttac caaggttggt
     8461 cgtccctatc gcgtgtactg gtgacgtcat ctgtcgatac attaaaaagg ctggagaaat
     8521 accagccttt ttgagtgctt atttgcgata cattttaaat tggaggtata aatcattata
     8581 aatccccaac catcttgacc ctaaattgtc ataaacttcc aattttttga taatgtcatc
     8641 tgttggataa aaagcaggat cattttttat ctcatctgga agtaaggcct tagctttttt
     8701 atttggtgtc gcataaccaa tatatgcagc attttgcgca gcattttcag gacgattgat
     8761 aaagttcaaa aaagcatacg cttctttttc gtgtttcatg gtttttggta gtaccaaatt
     8821 atcaaaccaa aggttagacc cttctgaagg cacgatgtag tgaaggtgtt cgttactatc
     8881 taacatctca ctggcttcac cagaaaaggt aattccaata gcagcgtcac cttgaatcat
     8941 gtagcctttc atctcatctg ctacaatagc tttaacattc ggcgtcaact gctgcagttt
     9001 tctctcggct gcctgcaact gctctagatt tttagaattc acactataac caaaagttgt
     9061 taaaccaacc cctagcattt cacgcgctcc atcaatcagc ataatactat ttttatattc
     9121 tggacgccac agatcttccc agtgcatagg tgccttatca actaattgat cattataaac
     9181 aatcccaacg gttccccaga aataaggcaa agaatagtcg ttttgtgggt caaagctttt
     9241 ccctaaaaat tctttaccga tattatccat gccaactaat tttgacttat caagcttatt
     9301 gagtaggttt tctttgatca ttttatcaat ggtgtaatca ctaggaacag caatgtcgta
     9361 agtggttccg ccctgcttga ttttagtgta catggcttca ttagaatcga aagtttcata
     9421 ctgcacttca atgcccgttt ctttggtgaa ttttttgagc aaagctggat caatgtaatc
     9481 tccccagtta taaataacta atttatccga ttgactacca gaacccgatt ttttctgcaa
     9541 gatgaaagaa aggcttgtta aaataacaat aacacccaaa actcctgcta gaaaggaata
     9601 aagtttacgc atgtttttcc tccttgtcct gtgacatata ataataaccg atcactaaga
     9661 ggatggagaa aaagaaaacg atggttgaca aagcattgat atccaaggaa attccctgac
     9721 gagcccgcga ataaatctca acagataaag tagtaaaacc atttccagtc aaaaagaaag
     9781 tcactgcaaa atcatctaag gaataggtaa aggccataaa ataacctgca ataatccctg
     9841 gtgttaagta tggcagcatg acttctttga gcatttggaa ataattagct cccaaatcat
     9901 aagcggcgtt gaccatgtct tgattcatct ctttcaagcg cggcaatacc atcaaaacca
     9961 caataggaat cgaaaaagca atatgactta ataaaactga agacatgccc agctgaaact
    10021 tcaatgatgt aaaaagaatt aaaaaggaag ccccaatcat gacatctggt gataccatca
    10081 agacattatt ggctgatagc atggcatttt ggtacttacc tctaacatgg tggataaaga
    10141 tagctccaaa ggtcccaata atcgttgcta gtagagcgct actaaaagca agaacaaagg
    10201 tttgcagtaa gattgtcatg agacgactat cctcaaacat ggtttggtaa tgctcaaggg
    10261 taaatcctgt aaaaccattc atatccccac ctttgttgaa agaatagaaa atcaaataaa
    10321 aaataggaat gtagagtaaa acaaagacac tcgctaaata aagattggca aattttttca
    10381 tttacttttc tcctttgtga gccacataat agcaaccatg gttaagatta acaccacacc
    10441 tatggttgat cccattcccc agttttgggt ggtcaaaaaa tgttgttcaa tggctgtacc
    10501 aagtgtaatc acgcggtttc caccaatcaa acgggttaac ataaagagac tcaaacttgg
    10561 gataaagaca gactgaacac ctgccctaac cccatttaaa gaaaggggaa aaataacttt
    10621 tgagaaggcc tgaaattcac tagctcctag gtcgcgactg gcattaatga cattatggtc
    10681 aatatcatcc aaagcgttaa aaatcggtaa catcataaaa gggagctcaa tgtaagaggc
    10741 tacaaaaatg aaggagaaat ccgtgaaaag gatttgctgc gggccaatcc ccataaaggt
    10801 taaaaagctg ttaattcctc cttgttgacc aaagattccc ataaaggcat aggcttttag
    10861 caataagttt acccaagttg gcaaaataat gagcataagc cacaactgct tgtgctttag
    10921 gcgcgttaaa aagagagccg taggatatga aatcaagagc gtgacgagtg tgataatacc
    10981 agcgtataaa atagaattca cactcattct caaataggtc caagagctaa aaaaggtttc
    11041 ataattggct aaggtcacgc gcccttctat gtcaaaaaag gacttgtaaa acaagagagt
    11101 gactggtgcc acaacaaaga agagaatcca taagaagtaa ggaatcgaaa aaagactaga
    11161 ggttttcttc attacgctcc tcctcaatgg cattaatcaa tccatcttca ggctcatcca
    11221 tttccacata ttcttcaata cgggcatcaa attcctcttc agtctcatta agacgcatga
    11281 tatgaagatc ttcaggggta aagtctaatc cgataacttc tccctcgata gctttgcggg
    11341 tagaatgaat catccattca ttacccaatt catcataggc aataatttcg tagtgaaccc
    11401 cgcggaataa ttgggtatca accttaactt gtaatttccc ttcttctggc aaagtaattt
    11461 gaagatcttc aggacgaata accacttcaa caggctcatt agggcgcatc ccaccgtcca
    11521 cagattcaaa ttctttcccg ttaaaggaga caagatagtc ttcaatcatg gtaccgttaa
    11581 taatattaga ttctccaata aaattagcaa caaaatgatt aattggctca tcataaatat
    11641 ccactggtgt tcccgactga acaatttcac cttcattcat gacaaaaacc caatcactca
    11701 tggccaaggc ttcttcttga tcgtgagtaa caaaaacaaa ggtaatgcct agacgttgtt
    11761 gcaattcacg taattcatat tgcatctctg ttcttaattt caaatcaagg gctgagagtg
    11821 gttcgtcaag taagaccaca cgcggttgat tgataatcgc acgcgcaatg gcaacacgtt
    11881 gacgttgacc accagataac ttctggatag aacgattttc atacccttct aattgaacca
    11941 ttttcaaggt ttctttcacg cgcttagcaa tctctttctt gtccactttc ttcagcttca
    12001 aagcaaaggc aacattctca aaaacagtca tatgagggaa gagggcataa ttttggaaaa
    12061 cggtatgaat gtcacgttta ttaatcggca aatcattaat acgttcccca tctaaataaa
    12121 tgtctccgct gctggcatct agtaggcccg ccataatatt taaaatagtt gattttccag
    12181 agccagaagc tccaagcagg gtgtaaaatt ttccttcttc aaggtcaaag ttaatgttct
    12241 ttagaacctg tgttccactg tcctcaaatg tttttgaaac attgttaaat gtgataattg
    12301 gcttagtcaa ttgtcataaa tcctccacgg tactttcttt aattatcaat aatggttaca
    12361 ggttttcccc aataatccga acttctggtt caagcctgac accagaatgg ttttcaactg
    12421 tctcaatgac ataagcaatc aaatcttcat aatctttagc tgtgccatct gacacattaa
    12481 tcataaaacc agcgtgtttt tcggaaactt ccacaccacc aatccgatgc cctttaagat
    12541 ttgcttccat gattaattga cctgc
//
LOCUS       DAUVUY010000056        12469 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.056, whole genome shotgun sequence.
ACCESSION   DAUVUY010000056 DAUVUY010000000
VERSION     DAUVUY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.056"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            159..428
                     /gene="rpsO"
                     /locus_tag="VOX58_001316"
     CDS             159..428
                     /gene="rpsO"
                     /locus_tag="VOX58_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HES5878543.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            781..2841
                     /locus_tag="VOX58_001317"
     CDS             781..2841
                     /locus_tag="VOX58_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HES5878544.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVTSGPWVFSYLLHELGLPDQTKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEVVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKPVNEDEIAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     VKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLFGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            2834..3118
                     /locus_tag="VOX58_001318"
     CDS             2834..3118
                     /locus_tag="VOX58_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HES5878545.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            3145..4524
                     /locus_tag="VOX58_001319"
     CDS             3145..4524
                     /locus_tag="VOX58_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HES5878546.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLAFARFTRFKYIFLTGHHSFFMACLLSAVLGAVDFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVLFYLVATVASVLRNTSVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            4534..5265
                     /locus_tag="VOX58_001320"
     CDS             4534..5265
                     /locus_tag="VOX58_001320"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HES5878547.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPVIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEEVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDDNH"
     gene            5545..7677
                     /gene="pnp"
                     /locus_tag="VOX58_001321"
     CDS             5545..7677
                     /gene="pnp"
                     /locus_tag="VOX58_001321"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HES5878548.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSILTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAINRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            7670..8422
                     /locus_tag="VOX58_001322"
     CDS             7670..8422
                     /locus_tag="VOX58_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HES5878549.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSVALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQESAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDNNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            8431..9012
                     /gene="cysE"
                     /locus_tag="VOX58_001323"
     CDS             8431..9012
                     /gene="cysE"
                     /locus_tag="VOX58_001323"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HES5878550.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTVRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            9491..9721
                     /locus_tag="VOX58_001324"
     CDS             9491..9721
                     /locus_tag="VOX58_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878551.1"
                     /translation="MNKIRVGLYVIMSLSILVSSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            9749..11092
                     /locus_tag="VOX58_001325"
     CDS             9749..11092
                     /locus_tag="VOX58_001325"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HES5878552.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKAIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFAIADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            11085..11498
                     /locus_tag="VOX58_001326"
     CDS             11085..11498
                     /locus_tag="VOX58_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HES5878553.1"
                     /translation="MINPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            11694..>12469
                     /locus_tag="VOX58_001327"
     CDS             11694..>12469
                     /locus_tag="VOX58_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878554.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASIRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAT
                     TNSVDVDTKAQTNQSANQEIDSTSNPFKSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLSGLVVSLAAGLFIAI"
BASE COUNT         3686 a         2336 c         2761 g         3686 t
ORIGIN      
        1 aggtggtttg gatgtgagag ctcagaataa taggataacg gttgacctaa tattatgaat
       61 ctgctagaat agagtggtcg aaagacaaac caactttttc ttggttttag gagtgccaat
      121 cctaagactc ggattaagca aaaaaaggag aatatattat ggcaatctca aaagagaaaa
      181 aaaatgaaat cattgctcaa tatgcacgtc acgaaggcga tactggttca gttgaagttc
      241 aagtagcagt tcttacatgg gaaatcaacc acttgaacag ccacatcaaa gaacacaaaa
      301 aagaccacgc tacttaccgt ggattgatga aaaaaattgg tcaccgtcgt aacttgttgg
      361 catacctacg tcgtacagat gttaaccgtt accgtgagtt gatccaatct cttggacttc
      421 gccgttaatt tcaagtttaa aaagtcctct gcttatgcag tgggcttttt catttttgac
      481 atgttgtttg ttaacttaca catttggcta gaatggtcta agactgtttt tttaagcagt
      541 tgatgatttt atttaacaac aaaaggtatc tcagtgatga ggtatctttt gttgtatcat
      601 gaagaaaaag ggaatcaatt cccagatttg cctatttttg gccacctttt gaggaacttg
      661 ttacaatgat atcacctgtt gagtccaatt gcagtcaaaa atgactacgg acaattggac
      721 tcaatttgtc ttgaaaatga aaacgcttca ttttatactg acattatagg aggagatgac
      781 atgctatctc atgagctcat cagaaactat caactctttt ctaaatataa aggacattca
      841 ctggaggcat ttgaatccat attaaagaca agcaaacgtc atatactggc agatattgct
      901 aaaatcaatg acacgttgtc actctatcag ttacccctta ttgctctgga caggcagctg
      961 gtttatccgc cagaccttac tgaaaaggac ttgttgaatc gcatgctacc taccttagac
     1021 gactatctct ttcaagatga gcgtctggac atgattatca tttacatcat gatggctaaa
     1081 gaatttatct ccattaacca cttggaaagc ttgttacggc tcagcagaaa ttccgttatt
     1141 gctgatttaa acttggtgcg tgatcgggta caagcttttc aggtaacttt agcttacaat
     1201 cgccaggatg gttatttttt tgaaggagaa cccttagcct tgcggcgtct cttagaatca
     1261 gcggtcagtt ctcttttgca agttacatca ggaccttggg tgttcagtta cttattgcat
     1321 gaacttggtt tgcccgacca gacaaaggtg atggcagcaa cgttggagga actgagcagg
     1381 gagaaccact taacttttat ttcggagaaa ttgagggatt tgatttattt tttctgcctt
     1441 ctcgctcatc gaccattttc acggaacgta agggctgaag tagtggatac ttttccttta
     1501 gcttcgccag ctgttgaaac gatggtggac cagttattgg tcaacttccc cagtctaacc
     1561 gaagagaaat atttggttca atctaggtta cttggttgta tccaaggtga cttagagtta
     1621 gtctttcagc aacctattta tgacattatg gaggaaatca tcaattcggt ggcggttaac
     1681 acgggattgt ctatcaccga taccccagaa cttcgtcaga acctatacag tcaccttttg
     1741 ccagcttatt accgacttta ctacgacatt aacttgacca atcctttgaa ggaacaaatc
     1801 aaacaagatt atgaatctct cttttacttg gtcaagcgca gcctttctcc tctagaaaaa
     1861 caattgggga aaccagttaa tgaagatgag attgcttatt ttaccattca ttttgggaga
     1921 tggttgcagg cccctaagaa gaggccgagc aatcagctgg tagccttatc tgtttgccct
     1981 aatggcatca gttcgtctct gatgttggag gcaaccttga aggaactttt tccacagcta
     2041 cagtttatta ggattcacca gctggacaaa ataaagttgt tggatccagc ttcctttgat
     2101 ttgatttttt caacagtagc ttttgactgt gttaaacctg tttatgtgac gcaagctttg
     2161 atgggacctg ttgaaaaaat gatgttgaaa aagatggtct gtgatgactt tcatcttccc
     2221 ttgtcagagc aattcgcttt ggatgacctg ttgagtatta ttcataaaca caccacgatt
     2281 actaataaag aaggacttgt tagtgattta tcccgttacc tgtttggtaa ccatttaacg
     2341 attgaaaaag gaggtctagg actattggac ttgttaacag cagattttat taggcaggct
     2401 gatgctgttt cggattggca ggaagcgatt cgtttggctg ctcagccttt actagaacac
     2461 cagatgattg aaacatctta tatcgacggt atgattgatt cggtcaatga gcttggagcc
     2521 tatattgttt tagcccctaa ggtggctgtt cctcatgcgg cgcctgaaaa aggaacgcgg
     2581 cagttaggca tgtctctctt acaactaaaa gaacctgtca gctttgattt gaagcaagaa
     2641 ggtgatccag acaagcaagt gcaattgatt tttgttttgt ctgctgtgga ttctagttca
     2701 catttgaagg ctttgcagga actgtcgttg attttagatg atgatgaaca tattgagcaa
     2761 ttaattgagg ctaagaatac tgaggaaata atgagtctga ttagccatat gattgaaaaa
     2821 ggagatgaat cacatgatta aaattgtaac ggtttgtgga aacggtattg gtagtagctt
     2881 gttacttcgc atgaaagtag aagctatcgc ctctagtttg ggtattgatg tggatgcaga
     2941 atcctgtgat tccaatgcgg ctgttggaaa gggtgcagat ttgtttgtta ccgttaaaga
     3001 atttaaagat atttttccag aggatgccaa ggtttgtatc gttaaaagct ataccaatcg
     3061 taaaaaaatt gaagaagatt tggttccggt tctcaaggaa atgagtggca aggaataagc
     3121 ctttaaagga gataggaaga tactatggaa gcattattat catttattcg agatatttta
     3181 aaagaacctg catttttaat gggcttgatt gcctttgcag ggttggtggc tttgaaaaca
     3241 cctgctcata aggtgttgac aggaaccttg gggccgattt tgggatacct tatgcttgct
     3301 gcaggggcgg gtgttattgt gaccaatctg gaccctcttg ccaaactcat tgagcacggt
     3361 tttagcatca ctggcgtagt gccaaataat gaagccgtca cttctgtcgc tcaaaagatt
     3421 ctgggtgtgg aaactatgtc catcttggtg gttgggttat tgcttaattt ggcttttgct
     3481 cgttttaccc gcttcaaata cattttctta acaggacatc acagcttctt tatggcttgt
     3541 ctcttgtccg ccgtgcttgg agctgttgat ttcaaaggaa gtcttttgat tatcttaggt
     3601 gggttcctct tgggagcttg gtcagctatt tcgccagcca ttggtcaaca gtatactctg
     3661 aaagtgactg acggagatga aatcgccatg ggacactttg gtagtttggg ctattacctt
     3721 tctgcctggg ttggtagcaa ggttggcaaa gacagtaaag ataccgagga ccttcagatt
     3781 tctgaaaaat ggagtttctt acgcaacacc accatttcaa caggacttat catggtgctt
     3841 ttctacttgg tggcaacagt ggcttctgtt ttgaggaata cttcagtaac agaagaatta
     3901 gcagcaggtc aaaacccatt tatctttgct attaagagtg gtcttacctt tgcggttggt
     3961 gtggcaattg tctacgccgg tgttcgcatg attttggctg acttgattcc agccttccaa
     4021 ggtattgcta acaaattaat tccaaatgct attccagccg tggattgtgc ggtattcttc
     4081 ccttatgcgc caaccgctgt tatcatcggt tttgcgtcaa gtttcgttgg tggcttactt
     4141 gggatgttga ttttaggagt tgcaggtggt gtcctgatta tcccaggtat ggtgcctcat
     4201 ttcttctgcg gtgcaacagc agggattttt ggaaattcaa caggtggtcg tcgcggtgca
     4261 atgattggtg ctttcgctaa tggcttatta ctcgccttct tgccagccat gcttctacct
     4321 gtacttggta aacttggttt ttcaaacacg acctttggag atgtggattt cggtgtttta
     4381 gggattttac tgggccgctt aggaacgtcg attggtcaaa tcggtattta cttggtggtt
     4441 gctgtgcttg ctattgtatt ggtgcttcca aactttctgt caaaatctca agaagccatc
     4501 aataatgttt cagaagaaat ctaaaaggag gatatgatga caaaaaaact aaacgttaaa
     4561 gtcttttcag acggtgcggt cttagagacc atgttaaagg atttacaaac aggtttggtc
     4621 acaggcttta caaccaatcc aagtttgatg aaaaaagcag gcatcagttc ttatattggc
     4681 tttgccaaag aagtgcttgc caaaattaca gactacccag tgtcctttga agtctttgcg
     4741 gatgatctcg caagcatgga aaaggaagct gagaaaattg ccagtctagg tgacaatgtc
     4801 tatgtgaaaa ttccagtcac cacttcaaca ggcgaatcaa cttgtcccgt tattcaaaag
     4861 ttgtcagcca aaggcattaa gttaaacgtc acagccattt ttacaattga gcagactcaa
     4921 gctgtggttg atcatttaac cgctggagtg ccagctattg tgtctgtttt tgctggtcgt
     4981 attgcagaca ccggcgttga cccaatgcca atcatggaag aagctctacg catttgtcgt
     5041 caaaaagaag aggtcgaatt gttgtgggct agtcctcgtg aaacttataa tatctatcaa
     5101 gctgatcagt taggcgtgga cattatcact tgtaccactg atttgattgc caagttgcct
     5161 cttcaaggaa aagatttaga agactattcc cttgaaaccg tgcaaatgtt tctgaaagac
     5221 agtaccagcc tcggctttaa aattttagag gatgacaatc attaatatta ggagacgaaa
     5281 gcaggcatac caacctgctt tttttgcttt gttaaagagt ataagtttcg tttaatattt
     5341 ttcaaggaca tttcaagaga ttgcgatctg acagtgaaat ttctgctatt ttatggtaaa
     5401 atgtagcaga tacgtcgttt gatacaaaga ggagcgtgtg acaagcgtgg caggttgctt
     5461 tttgtagcaa aacaacgtat aacaactgaa tctaagcaga gaacaggtac ctctttaccc
     5521 ctctgaaaat taaaaggaga atatatgtca aaacaaacct ttacaacaac atttgcaggg
     5581 aaaccccttg ttgttgaagt tggtcaagtc gctaagcaag ccaacggggc aaccgttgtt
     5641 cgttacggag aatcaaccgt cctgaccgca gctgttatgt ctaagaaaat ggcaacgggt
     5701 gatttcttcc cccttcaagt taattacgaa gaaaaaatgt atgccgctgg taaattccca
     5761 ggaggtttca tgaagcgtga gggacgccct tcaacagatg cgaccttgac agctcgcttg
     5821 attgaccgcc cgattcgtcc catgtttgcc gagggttttc gtaacgaagt acaagtgatt
     5881 aacactgtcc tttcttatga tgaaaatgct agcgctccaa tggcagcgat gtttggctca
     5941 tctctggcct tgtccatctc agatattcca tttaatggac cgattgcagg tgttcaagtt
     6001 ggctatattg atggtgagtt catcattaac ccagataaag aacagatgga agcgtcactc
     6061 ttagaattaa ccgttgctgg tagcaaagaa gccatcaaca tggttgaatc tggtgccaaa
     6121 gaattatcag aagacatcat gcttgaggct cttttaaaag gtcaccaagc cattcaagaa
     6181 ttaattgcct tccaagaaca aatcgtagca gttgttggta aagagaaagc tgaggttgaa
     6241 ctgttacagg ttgatgcaga cttacaagct gatattgtgg ctaagtacaa tgctcaactg
     6301 caaaaagctg ttcaagttga agagaaaaaa gcgcgtgaag cagccacaga agccgttaaa
     6361 gaaatggtta aggctgagta cgaagagcgt tacgcagaag acgaaaacct agcaaccatt
     6421 atgcgtgacg tggcagaaat ccttgaacaa atggagcatg ctgaagtgcg ccgcctcatt
     6481 acagaggaca agattcgtcc tgacggtcgt aagattgacg aaattcgtcc gcttgatgct
     6541 gtggttgact tcttgccaaa ggtccatgga tcaggtcttt ttacgcgcgg tcaaactcag
     6601 gcgctatcaa ttttgacctt ggcaccaatg ggagaaactc aaatcattga tggtctggcc
     6661 ccagagtaca aaaaacgctt cttgcatcac tacaatttcc cgcaatattc agttggtgaa
     6721 acgggtcgtt acggagctgc tggtcgtcgt gaaattggac acggtgcctt aggtgaacgc
     6781 gctcttgaac aagtcttgcc aagcttagaa gagtttcctt atgccattcg tttagttgcc
     6841 gaagttctag aatctaacgg ttcgtcttct caggcttcta tctgtgctgg tacccttgct
     6901 ctgatggctg gtggtgtacc aatcaaggct cctgtggcag ggattgccat gggtctcatt
     6961 tcagacggga cgaattatac agtcttgacc gatatccaag gtcttgaaga ccactttgga
     7021 gatatggact ttaaagtggc aggaactcgt gaagggatta cagcccttca aatggatatt
     7081 aagattgcag ggattacgcc tcaaatccta gaagaagctc ttgcacaagc taaaaaagct
     7141 cgctttgaaa tccttgatgt gattgaagca actatcgctg aaccacgtcc cgagttagcc
     7201 ccaactgcac cgaaaattga caccatcaag attgacgtag acaaaatcaa agtggttatc
     7261 ggtaaaggtg gcgaaaccat tgataagatt attgccgaaa cgggtgtcaa aattgatatt
     7321 gatgacgaag gaaatgtctc tatctactca agtgaccaag ctgccattaa ccgcactaaa
     7381 gaaatcatcg ctggcctagt acgtgaagct aaggtaggcg aagtttacca tgctaaggtt
     7441 gtccgtattg agaaatttgg tgcctttgtc aacctctttg acaagacaga tgcacttgtt
     7501 cacatttctg aaattgcttg gactagaacg accaacgtgt cagatgtcct tgaagtgggt
     7561 gaagacgttg atgttaaggt cattaagatt gatgagaagg gtcgtgtgga cgcctcaatg
     7621 aaagctttga ttccacgccc accaaaacca gagaaaaaag aagaaaaaca tgactaaatc
     7681 aaatgaatta gatattcgct taagagcttt tatcaatgcg cctgataact ttttggatag
     7741 tgttgccctt gtcaatgctt tccataattt tcctgtctgg gctgctaaag aaccctacgt
     7801 gatagaagtc gaaggtgtca aggttacccc tgtttttacc gataaggaag atatggctcg
     7861 ctttaaagaa gaacaagaga gtgctcaaag tcagtattgg ctggagcgtt cagctcttgc
     7921 agtgctagaa gaagtcatta catctggtgc agcaggtctt gtctttaacc ttaagaaaaa
     7981 gggagatttt gggaattcta ccattttcaa aagcagtgat atgattcaat ttatgaacca
     8041 ttacaccact gtgttaaata cgcttatgag tgatgacaat gtggcggcag atacgatgga
     8101 aaaggtttac cttgttcctg cctttgttta tcctaaagat aataaccatt acgaccgcct
     8161 ttttcctacc atgtcaacgc ctgaaggaaa aagctatgtt cccgcctttt caaatcttca
     8221 aagctttgcg aaatggtaca accaagacga ttttggaggc cttttcagga aagctgaagg
     8281 agtgattctg acttggacga ttgatgatat ttatcaacca agaaatggtg aaaacgagct
     8341 tgatgaaacg ttcggtgtcg ccatcaatcc ttttgatgac caacaaattc ttgttgattg
     8401 gtcagaatta gataagtcgt aggagttagt atgggttggt ggaaagaaag tatagctatt
     8461 gttaaagctc tagacccagc tgctcgtaat agtcttgagg ttatcctaac ttatccaggc
     8521 atcaaagccc tagctgctca tcgtttgtct cattttcttt ggcgacatca ttttaaatta
     8581 ctagccagaa tgcatagcca attttggcga ttttggacac aaattgaaat ccatcctgga
     8641 gcacaaatcg ctcctggagt cttcattgac catggtgctg gtcttgttat tggagagaca
     8701 gcaattgttg aaaaaggtgt gatgctttat catggggtga ccctaggtgg aaccggaaaa
     8761 gattgtggca agcgtcatcc aacggttcga caaggtgcct taatttcggc acatgcccaa
     8821 gtgattggac ctattgacat cggagcaaat gctaaagtag gggcagcagc tgttgtgtta
     8881 tcagatgttc ctgaagacgt gacagttgta ggtgtgccag ctaagatagt acgagtgcat
     8941 gggcaaaaag ataatcgtca aattcaaagt ttacaaaaac aacgagaggt ctcttatcag
     9001 ttgtcaaaat gatcggaaaa taacaaaaag aagcactgat gacttccgat aaaatatttt
     9061 gggactttct agtagagtaa tgttgaactt agctagccct atcagtttgg ttgctgagtg
     9121 tagtgatatg ggaatgttac aaaatacaaa aaacacgtaa aagagcatca actaagcaag
     9181 tcttagactg aagaaacagt ctattgtgat actttttctc aggtggtgct tacggcagcg
     9241 acttcctttg gaattccatg ccttagtttt gagcctaagg tctcaaaaat ctgtatgggc
     9301 atcactgtca gtattgatac cacttttacc acaacaaaaa gcagctgttt tggctcaccc
     9361 cacatgcaga aataggaaac ctgatgtcaa atcagcagat ctattcatgc tttttgagca
     9421 taaaagtggg tttgtctact ttctgaagcc ttcacaggtt gtgaaggttt ttcgttaata
     9481 aaggagcctc atgaataaaa taagagttgg gctgtatgtc attatgagtt tgtctattct
     9541 tgtttctagt attacactgg tggctagtct aatcttttta ctgagacaag acatggttaa
     9601 agcgattata tacttcattt tgaccctatt ttggttaata ggtttggtta ttaatattaa
     9661 attgttgaaa aggtggaata gcatttcaga aaaagactta ggatatcaag atgacaacta
     9721 atgctaggat attgaataag gagtttacat gattaaaatt tatgatacca tgacccgttc
     9781 gctccgcaag tttgtacctt tgactgaaaa tacagtcaat atgtacgttt gtggaccgac
     9841 ggtctataat tatattcata ttggaaatgc tagatcagcg gtcgcttttg acaccattcg
     9901 acgttatttt gagtatactg gctatcaggt caattacatt tccaatttta ccgatgtcga
     9961 tgataaaatt atcaaggctg ctactcaagc aggtgtttct cctaaagaat tgtcagatcg
    10021 ctttattgca gcttttatag aagataccaa ggcacttggt gttaagccag ccacacaaaa
    10081 tcctcgtgtt atggattata tagcagaaat catttcattt gttgaaagcc tcattgaaaa
    10141 agattttgct tacgaagcag atggagatgt gtatttccgc gtggaaaagt cagagcatta
    10201 tgccaagcta gctaataaaa ccctgtcaga acttgaagtt ggagccagtg gtcgaacaga
    10261 tgctgagaca gctttaaaag aaaatccact ggactttgct ctttggaaat cagctaaggc
    10321 aggtgaggtt tcttgggata gcccctgggg gtttggtcgt ccaggctggc acattgaatg
    10381 ttctgtgatg gctactgaaa ttcttggtga taccattgat attcatggtg gcggagctga
    10441 tttggaattt cctcatcata ccaatgaaat tgcccaatct gaggctaaaa caggcaagac
    10501 ttttgctaac tattggatgc acaatgggtt tgtcactgtt gataacgaaa aaatgtccaa
    10561 gtcactaggt aattttgtga ccgttcatga catgttacaa accgttgatg gtcaggtttt
    10621 gcgattcttt cttgcgacac aacagtaccg aaaaccaatt aacttcactg aaaaggctat
    10681 tcatgatgcg gaaatcaatc tgaagtatct taaaaacacc ttgcaacaac cgctgacaga
    10741 aactgcagat gagcaagagc taaaacagtt tgtaatagct ttccaagacg ctatggacga
    10801 tgatttcaat acagcaaatg ggattaccgt cgtatttgac atggctaagt ggattaattc
    10861 tggctcctat acagaacctg ttaaaagcgc ttttgagaaa atgttagctg tttttggcat
    10921 tatctttgaa gaagaggtgc ttgaggttga cattgaagcc ttgattgcta agcgacaaga
    10981 agcacgcgcg aatcgtgatt ttgccatagc tgatgccatt cgagatcagt tagcagctca
    11041 aggcatcaag ctgctagata ccaaagatgg tgtgaggtgg ctgcgtgatt aatccagttg
    11101 atgtgaattt gattaatggc attgccctag cctttgaagg agatgcggtt tattcctact
    11161 atgttcgtcg tcatctcatt tttcaaggta aaacgaaacc tagccagcta caccgtttag
    11221 caacgagata tgtttctgct aaggcacaag ccaacttgat tcaggctatg ttagaagcgc
    11281 agctattgac cgaaaaagaa gaagacatct ataagcgtgg tcgcaatacc aatagccata
    11341 ctaaagctaa gaatgccgat attattacct atcgtatgtc aacaggtttt gaagccatta
    11401 tgggttatct tgatatgatg ggccaaaaag agcggttaga agaattgatc agatggtgta
    11461 ttgagtatgt agaaaagcaa caattgatat cctcataaga gtcccctagg agactcttat
    11521 tttcataggt ctgaagtaaa ggtagagagc cattaactct ttttatgaca tcaaaaagac
    11581 ataatgatat aaaaaagata catttgtaac aaaaaagaca taaaaataat gaaatcattt
    11641 tcgaatagtt tataatgaga gggtaagact aatttaaaga ggagatattt attatgacaa
    11701 ctatgcaaaa aacagttagc ttattatcac tagctttact tattggtttg ctggggactt
    11761 ctggcaaagc catatctgtg tatgcacaag atcagcacac tgataatgtt atagctgaat
    11821 caactattag tcaggtcagt gttgaagcca gtatacgtgg aacagaacct tatattgatg
    11881 ctacagtcac cacagatcaa cctgtcagac aaccaactca ggcaacgata acacttaaag
    11941 acgctagtga taatactatt aatagttggg tatatactat ggcagcgcaa cagcgtcgtt
    12001 ttacagcttg gtttgattta actggacaaa agagtggtga ctatcatgta actgtcaccg
    12061 ttcatactca agaaaaggca gtaactggtc aatcaggaac tgttcatttt gatcaaaaca
    12121 aagctagaaa aacaccaact aatatgcaac aaaaggatac ttctaaagca acgacgaatt
    12181 cggtcgatgt agacacaaaa gctcaaacaa atcaatcagc taaccaagaa atagattcta
    12241 cttcaaatcc tttcaaatca gctactaatc atcgatcaac ttccttaaag cgatctacta
    12301 aaaatgagaa acttacacca actgctagta atagccaaaa aaacggtagc aacaagacaa
    12361 aaatgctagt ggacaaagag gaagtaaaac ctacttcaaa aagaggattc ccttgggtct
    12421 tatcaggtct agtagtcagt ttagctgcag gtttatttat agctattca
//
LOCUS       DAUVUY010000057        11532 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.057, whole genome shotgun sequence.
ACCESSION   DAUVUY010000057 DAUVUY010000000
VERSION     DAUVUY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.057"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(93..530)
                     /locus_tag="VOX58_001328"
     CDS             complement(93..530)
                     /locus_tag="VOX58_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HES5878555.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            complement(517..2649)
                     /locus_tag="VOX58_001329"
     CDS             complement(517..2649)
                     /locus_tag="VOX58_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HES5878556.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            2883..3038
                     /locus_tag="VOX58_001330"
     CDS             2883..3038
                     /locus_tag="VOX58_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878557.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            3186..4088
                     /locus_tag="VOX58_001331"
     CDS             3186..4088
                     /locus_tag="VOX58_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HES5878558.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKTFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            4088..4903
                     /locus_tag="VOX58_001332"
     CDS             4088..4903
                     /locus_tag="VOX58_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HES5878559.1"
                     /translation="MIRFLILAGYFELTMYLQLSGKLDQYINVRYSYLAYISMVLSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLTEVHQTKEPNNPYVYRVF"
     gene            complement(5002..6426)
                     /locus_tag="VOX58_001333"
     CDS             complement(5002..6426)
                     /locus_tag="VOX58_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HES5878560.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADTKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMDFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(6445..8307)
                     /locus_tag="VOX58_001334"
     CDS             complement(6445..8307)
                     /locus_tag="VOX58_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HES5878561.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLTLFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFINALIAGAATIVLAFSLTWFMGIDEE
                     SPKQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            complement(8309..9151)
                     /locus_tag="VOX58_001335"
     CDS             complement(8309..9151)
                     /locus_tag="VOX58_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HES5878562.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLLVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            9479..10846
                     /locus_tag="VOX58_001336"
     CDS             9479..10846
                     /locus_tag="VOX58_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HES5878563.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGAKLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            complement(10900..>11532)
                     /gene="ftsY"
                     /locus_tag="VOX58_001337"
     CDS             complement(10900..>11532)
                     /gene="ftsY"
                     /locus_tag="VOX58_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HES5878564.1"
                     /translation="NYQDGLTVMLFVGVNGVGKTTSIGKLAYRYKQEGKKVMLVAADT
                     FRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVAKGVDILLIDTAGRLQN
                     KENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQAKEFSKITPLTGLILTK
                     IDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFMKGLLEGIL"
BASE COUNT         3305 a         2531 c         2138 g         3558 t
ORIGIN      
        1 tttcgctgtt tatagaattt agtttccacg tttctttacc tcctcattct atgatactag
       61 tttacattgt ttttcgcaaa ataaaaccag tcttacgact gatttttttc cataagtttc
      121 ccgtggcaat ttccacaaac atatttagcc agattgatac gcctttttcg ttgataaacc
      181 tgcccacagg tttggcaact ataatgatga ttagtctttg atttagagct agttgggaca
      241 taacgaagcc cgtttacttg agctagcaga tctttgaaat ccctatcttt atgatggtac
      301 cctcttcctt caaaataaag atggtaatgg cagagctcat ggcgaacaat tttccgaaaa
      361 ataagctcac cgtgctcctc aagcatccta gggttaaaat ccaaatgacc atcttttgga
      421 aaaaagcgac cgccagttgt ttttaggcgc ttgttccaat aagccttgtg gtgaaatggt
      481 ttgccaaaat cagccaggga aacttcttgg acataattag tgagtgtcac gcggtggcaa
      541 taggctgaga ttgaccttgt ggcggtccaa gtcaatctta gagacccaaa cagttaccaa
      601 gtcacccact gaaacaactt gactagggtg gttgacaaag gttttgctca tttcagaaat
      661 gtgaataagc ccatcttcat gaacaccaat gtctacaaaa gcaccaaagt caacgacgtt
      721 tctcacagtt ccttcaagct tctggccaat ttccaaatct ttcaaatcaa ggatatcttg
      781 acgtaagatt ggtgcttcaa agtcatcacg aaggtcacga cctggtttaa ggagatcagc
      841 aatgatatct ttaagggttt cctgcccaat agccaatgtt tctgccattt ggggaacttg
      901 aactgctgct aaagttgcct tggcagcgtc gtccaagtcc tgaataccaa gtaccttaaa
      961 gagttctttg actgctgggt aggactcggg gtgaactcct gtgttatcta aaatattttt
     1021 ggctcctgga attctgagaa aacctgctgc ttgctcaaac gccttagctc ctaaacgcgg
     1081 cacttttttg atgtcagcgc gtgatgtcaa tgctccattt tcttcgcggt atttgacaat
     1141 attttctgaa atcgttttgt tcaaacctga cacatgtgct aataaagatg gactagccgt
     1201 gttcacatta acaccgactt ggttaaccac agtatccaca acaaaaccaa gattctcact
     1261 caactttttc tgactcacat cgtgctggta ctgaccgacg ccaatagatt taggatcaat
     1321 tttaaccaat tctgctagtg ggtcttgcaa gcgacgagca atagaaatag cagagcgctt
     1381 ctccactgtg aggtctggaa actcatgacg ggctaactct gacgctgaat aaaccgaagc
     1441 accgctttcg ttaacgatga cataggacgt atttgggaaa tctttcaaaa cgtctgctac
     1501 aaaggcttca ctttcccgac tggctgttcc gtttccaatg gcaataatat caatctggta
     1561 agtctcaatg agctgagtca gtgtttcttt agctgcttga atcttcgttt ggctagctgg
     1621 tgctacggga taaataacct gagtagtcaa caattttcca gtctgatcaa cgatagccaa
     1681 ttttgcacct gttcgaaagg cagggtcaaa cccaagaacc attttccctt ttaaaggaga
     1741 cactaacaaa agatgacgga gattttcaga aaagaggtgg atagccccat cttctgcggc
     1801 atcactgagt tctgaacgaa cccgtctttc catagctgga acaattttct ttttgatggt
     1861 ctgattgatg acctcttcaa tataagggtt ggtttctttg aaccgcacac tgaaaaagcg
     1921 ttgcattttc tccaggttat gctcaaaaga cacttttaag atgcctaact tttcgccacg
     1981 gttaagggct aaagtacgat atccttgcat gttagacact tggtcagaaa aatcgtaata
     2041 gatttggaaa acctttttct catccaactg ctcatcttta agcgttgata ctaaacggct
     2101 gtactgccaa atctcattgt aggtccaaga gcgtaattta gcatcttctg acatggcctc
     2161 cacaagaatg tccacagctc ctgctagagc ttcttgtggg ctggcaaatc cttctgtgac
     2221 aaagggttct gctgctgttt caaggttttg cgcattttgt aaaatcaaac gtgctaatgg
     2281 gaacaagcct gcttcacgcg caatcgtcgc ttttgtacgg cgtttttcct tataaggcag
     2341 gtacaactct tccaaatcag cgagtttttc ggttgcttca atactcgttc gtaactgatc
     2401 tgtcagtttt ccctgttcct caattttagc gagaatggtt gctttgcgct cattcagggt
     2461 agtgagagac ttatccatat caataatgga cttaatcacc acttcatcta gatttcctgt
     2521 tacttcttta cggtaacgtg caataaaggg aatggtattg ccttgtgctg ttagggcaag
     2581 cacctgttca atttgatgga gactaactga caaagcctct gcaatgttgt gattattgtt
     2641 attttccata atattgctat tataacacaa accatgttaa tgcaaaaaat gatagcgaaa
     2701 cattgtccta atataaagtt gcgtaaagaa tcaaaaaaag ctacttttag taaataactg
     2761 acgaaaccct aaataacaat acctaatttt aaaaaagaaa gaagtaaaca gcacttccgc
     2821 tttggcattg ttctcaaatg atagtataat agacttatct tgaatgaaag aaggtcatag
     2881 atatggcaat tacccataaa aaaaatgatg aacttgaaaa aatgctagca ggctttgcat
     2941 caatccctag ttttgataag cctttggaag tcaatactga tggtaaacta gcgaccaaag
     3001 aaaaaacgac agaacaaaca aaaccaagca aaccatagct agtttgtctc attctcttaa
     3061 catatcgtcc attcgtttaa actgagttga gagattttta gcgatcaggc taacaaaacg
     3121 tatcagtcga ggctgaacat tcgtgtttca gcctcctctt ttatcatcta gaaaggatat
     3181 ggtgaatgtc actcttttcc aaccttcctc ccagtgtctt gcaatggttt gcgattttta
     3241 tgtccattat cattgaagct cttccttttg tgctcctagg aactatttta tctggttgta
     3301 ttgaagtctt tgtaacgcca gaattggttc aaaaatacct acctaagcaa aaatgcttgc
     3361 gcattctgtt tggaaccttt gttggatttg tttttccttc ttgtgaatgc gggattattc
     3421 ccatcatcaa ccgctttttg gaaaaaaagg tgcctagtta tacggccgtc ccttttctgg
     3481 caacagcgcc tattattaat cctattgttt tgttcgctac ttattctgct tttgggaatt
     3541 ctttgcgctt tctgatactt cgcttggtcg gtgctgcact agtagccatt acacttgggg
     3601 taatgttagc ttttattgta gatgataaca ttctcaagga taatgctcaa cccgtgcatt
     3661 ttcatgatta ttctcatgaa agcttgccaa aacgcattta tctagcactg gtacatgcta
     3721 ttgatgaatt cttcgacacc ggtaggtatc tcgtatttgg aaccttgatt gcctctgcca
     3781 tgcaaatata tgtcccaact cgtgtgttga caacgattgg acacaatccc ttgacagcca
     3841 ttttgatcat gatgctgatg gctttcattc tatcattatg tagtgaagcc gatgccttta
     3901 tcggagcttc cttattatcc acctttggag tggctccagt tcttgccttt cttttaattg
     3961 gtcctatggt ggatattaaa aaccttatga tgatggtaaa gacttttaaa ggacgcttca
     4021 ttgtccaatt tatcggcgta tccgttctta tgattgccgt ttattgttta cttgtggggg
     4081 tgctctaatg attcgctttt taattttagc tggctatttt gaactaacca tgtatctcca
     4141 gctgtctggc aaactggatc agtacatcaa tgttcggtat tcttaccttg cttatatttc
     4201 catggtttta tctttcattt tagctcttgt tcaactctat acctggatga aaaacattaa
     4261 ggtacatagc catttaaccg gaaaaatcgc aaggctgacc agtcctttta tcttggtctt
     4321 tcctgttttg attggtctcc ttgtgccgac cgtaacctta gattccacca cggtttctgc
     4381 caagggttac acttttcctt tggctgcggg agcctctaaa acgggagtca gtgatgacgg
     4441 gaccaccatt caatacctca aacctgatac cagcctttac tttacaaagt ctgcctacca
     4501 aaaggagatg cgtcaagaac tccataaata taaaggcaag aaaccggtca ccattaccac
     4561 tgaaaattat atggaagtga tggaattgat ctatctctac ccagatgagt ttcttgatcg
     4621 tgacatccaa tacactggct ttgtttataa cgaaccgggc catgacaact accaattcct
     4681 tttccgcttc ggcattattc actgtattgc cgactcagga gtgtacggac ttttgaccac
     4741 aggtaatcaa accagctacc ctaacaacac atggctaaca gttaaaggtc gccttcatat
     4801 ggaatatgat aaaaacttag aacaacatct acctgtcctt caacttactg aggttcatca
     4861 aaccaaagag cctaataatc cttatgttta ccgcgttttt taaagactaa taaaaaagca
     4921 cttgtttttt atcaaaacaa atgctttttg agttttatca gttagcatag gaacagagtc
     4981 accatcttaa acaagaccta tttaggaaaa attatcctca aggtaacgtc cattggtttg
     5041 aatgacttgt cggtaccaat caaacgattt tttcttatag cgtgtgagtt gaccagtacc
     5101 atcatcattt cggtcaacat aaataaaacc gtaacgctta cttaattggg ctgttgacat
     5161 agagacacaa tcaatacagc cccaagcagt gtaacccatg acctcaacgc catcttcaat
     5221 cgctttggca acttgcacga gatgctgact catgtagtca atgcgataat catcgtgaac
     5281 cgtcatgctg ccatctgcgg tttgcaccag ctgatccttt gcacctaagc cattttcaac
     5341 aataaacaaa ggaatctggt agcggtcata gtactgattc aagaccaatc gcagaccaat
     5401 tgggtctatc tgccaacccc actccgaaga ttctaaatat ggattggaca aaccacctag
     5461 aatatttccc tgaccagatg tgtaagcttc aggattgtgg gcttgtgtga cactcatgta
     5521 atagctaaag gatagaaaat caaccgtatg ttccgctaat aattccttgt ctccgtcttc
     5581 aaaaacaatc tcaatcccat tttctttaaa atacgactta atatatgaag gatacttgcc
     5641 tctcacatga atatctgaaa ataagagatt aagattttca aatgcatgcg cagcaagtat
     5701 atcacgagga tcagaggtca ttgggtaagc aggcatcgcc aaaatcatac acccaacttt
     5761 aacatcagga ttgatttcat gcgctagctt tgtgacacta gcagatgcta ccaattcatg
     5821 atgaatagct tggtataaat cttgcaggct cagcttttct ttttctgtca tgatgccacc
     5881 actggtaaag ggcatgtgta aaatcgagtt gacttcgttg aaggtcaacc agtatttgac
     5941 tttgtcctta tagcgttcca tgactgtctg agcaaagcgc tcaaaaaagc caatcagacg
     6001 tctatctgtc caaccattat aggctttggc caaatgcaga ggtgtctcat aatgcgacaa
     6061 ggtcactaag ggttcgatac catagttcaa caattcatcg aataagtcat catagaattg
     6121 caaacctgct tcattgggct ggtcatcgtc accatttgga aaaatacgag accaagcaat
     6181 agatgttcga aagactttaa agtccatctc ggcaaaaaga gcaatgtctt ctttgtagcg
     6241 gtggtaaaaa tcaatcgcct ctaaagttaa attatcgctg gtaggactat ctgtccattc
     6301 tcctagtcca ccatttggta gaacatcttg tacggacaaa ccttttgtgt ctgcctcaaa
     6361 tgctccttcc acttggttgg cagctactgc gccgccccat aagaaatctt ttggaaaaat
     6421 tcccattctc cctctccttt taggttataa aatgactaac aaagcgtcct ctttagtcac
     6481 catttttttg tcatcttgaa taataatctc caactgctca gccgaattgg tcacgaccac
     6541 aggactgatg agtgaatagc cttttgaagt aatgaaatca atatccattt ttagaagtgc
     6601 ctggcctcgt ttcacgacat cgccaacaga cactaactgc tcgaaacctt gcccttttaa
     6661 ctcaaccgta tcaataccga catgaatcaa gacttccaca ccacgtgtgt tttttaaggc
     6721 aacagcatgc ttggttggaa agaaagtgat gacttcacca tcaaaggggg catagaccac
     6781 accttcgctt ggtaatatgg caaatccttc tcctaataac ttggaagaaa aggtttcatc
     6841 aggaacttct gatagaaaaa gcatctcacc tgtcattgga gcatacaacg tttgtttggt
     6901 tgacaagccg ctcttaactt gtgacatatc tgctgcaaca ctcacttgct ttggggactc
     6961 ttcatcaatt cccatgaacc aagtcaaact gaaggctaac acaatcgtcg cagctccggc
     7021 aatcaaagca tttataaaat tagctccgcc tgatgggttg atgtactgtg gtaaaccaat
     7081 aatagaaggt acgacaaagg tataagaagc aatattgaca aaaccaataa aggctccaac
     7141 cagaccacct gaaatcatgg ctgcatagag tggcttttta aatttcagtg tgaccccata
     7201 aagtgccggt tccgtgatac ctgccagcaa agctgaaatc cccgcggcaa gggcaacttg
     7261 acgtgtttgt ttctgctttg ttttaaaggc aacagccaaa gatgcggctc cttgagccaa
     7321 attagaagcc aacattgctg gtaaaatcaa gacatctggt gttgcgaccg aagcggccaa
     7381 aaagattggt gcaaaagccc agtgcatccc cgtcatgaca acaagcggca taatcgctcc
     7441 caaaatggca agtgctagcc acggagccac atgataaata gcgagcatgg tattagataa
     7501 cccttgacca ataatcacac caagaggacc tacaaccact aaagccaaaa agccagaaat
     7561 caaaatgatt aaggttggct gtaagaagct tttcatcacg cttggcacca agcgattgac
     7621 ccatttttca atgtattgca tcaagtaaac catcatcaaa atgggaataa cagatgagcc
     7681 atagctagca ggagtgactg gtgctccaaa taaagtcaat ggtttacctt ctgctaccat
     7741 tgcaacaaag gcaggatgca ataaaatccc tgcgatcgtc gctgctaaaa caggtgtcac
     7801 cttaaagcgc gccgctgctg aataggcaat catgacaggc ataaaataat aagccgcatc
     7861 gccaaagaag ttaataaagg caacggtttg agagtccgca ctcgctaagc caagcatagg
     7921 aagcaagatt cccaccactt tgagcattcc accaccaaga agggctggaa tcagcggtgt
     7981 aatcacgcca gcaatggttt caatcaagcg ctcaaggata cttcctttcg cttttgaaga
     8041 atgatcttca tcttgattgt caaaatgccc taggcttaaa aaggcttggt aataattatt
     8101 gacatcattg cccaagataa tttggtactg gccgtttttg cgcatcacgc caatcacatt
     8161 tgaaatggct ttgacttgct gatctttaac tttctcatca tttttcagta ctaagcgtag
     8221 acgtgttaca cagtgtgtca cacgttgaat atttgttttg ccaccaaccg ccgctaaaat
     8281 agccttggca gtttcttgat acgtcatatt aagtctcctt taaatgtttc actaaacgtt
     8341 taacatgaac cgtcaaatac aaaagttcgc ttgaattaag gtggtagttg taccgctttt
     8401 ttatctcctc actaatatct aagacgcacc ggtattcttt agggtatttc gttttcacca
     8461 ctaaaagcag ttccgcatca tcatcatcgt aagacatctc tgataacaca cgttgagcaa
     8521 ataatttaat atgggtcata aagcggtaat aatcgatgga tgtgtcatcc aattctgttt
     8581 tgaagtctgt tttgattttt tgctcaatat aagaaacaat ctccgtcagg cgatgtggct
     8641 ccttaaaagg agtgtccaaa ctagcattga caaaatgcat ggcaataaaa gctgcttcat
     8701 caatagctag agtgactcct agattacgct caattagctc taaggctttt acccctagac
     8761 tatattcatc aggataatag cgttgaattt cccaccgtag tgggttatga atcaacattc
     8821 cttgctcatg cctttcaata gcagagtgaa tatgatccgt taaattaata tagagaattt
     8881 catctaaggt tttgcctaat ttaattttac ctagattgat aattctctct gagcaagcaa
     8941 caacttcttg tggcaccgta ataaataact ctgtgaagcg attcatattg tcactatttt
     9001 ttaaaacaaa tgatgtttcg atagcattaa gctcaatact atctccaact ttttttccaa
     9061 aggcaatccc ttttcccatc aataaaatat ctaatccttg atggttggtc gaaatcgccg
     9121 cattatggtt taaaacgcgt ttaatcaaca taaaagtcct agtcctttag tatcacaata
     9181 aaaaatgacc tatccaaaag ctcgctaaaa agcacttttc gaataggtcc ctgctcacaa
     9241 aactgtgata acatccttta atattctgat tgttatgtct ctagcctgct tacttgaagt
     9301 aatcacatct gagaacgctt taatgttatc atctctttaa gattctgtca agcgttttca
     9361 aaaaattctc tgaaagagca actattgtta ttcgtatcta ttgatttagc aaaaaagagt
     9421 ttctgacaat tttaagatta tatgatacaa taacaatcga ttagataaag gagttatcat
     9481 gtcacatcat caacaaaccg tttcaaaaca aacaattatg gcgattatcg ccatagcact
     9541 cattggtttt tcaggaattt tgtctgaaac cagtatgaat gtcaccttcc cgacactgat
     9601 gtcagtctat cagttaccct taaatagctt gcaatggatg acgaccattt atttactagc
     9661 agtggcgatt atgatgacca cttcggctac actgaaaaaa aatgtgcggg aaagacccct
     9721 cttttttatg gccacaggtc tttttacatt tggcaccatc ttggccgttc tgacccagtc
     9781 ctttgcgatc atgttgctag cccgcatttt tcaaggcatt ggtactggtc tggtaatgcc
     9841 tcagatgttt aatattattt tagagcgtgt cccaatgcat aaggtaggtc tatttatggg
     9901 atttgctggt cttattatta gcttagcacc tgcttttggg cctacttatg ggggctttat
     9961 gattagccat tttagttggc aatggatttt tatctgtata ctccctgtac cactgattgc
    10021 aggtattcta gcttattatt acctcgaaga ttctccagta agcgaaaaag taccctttga
    10081 ctggttggca tttattgcac tatcgattag cttaacttct gccttattag ctattactag
    10141 tctagaaaac ggaagtgtta atttgtatta cttagggctt tttattctca gctttgtcct
    10201 cttcctctac aagaatctta cagctaagca accctttctt gatattcgca ttctcaaaat
    10261 tccctctcta acctttggcc tgattccctt ttttgtcttc cagctgatta atttaggcat
    10321 aaattttcta acgccaaact ttattgtcat ggaaaagatt gctaatagtt ctcaagctgg
    10381 tatggtgtta ctacctggta ccttactcgg agctctacta gcacctgctt ttggtaaact
    10441 ttatgatcaa aaaggagcaa aactttcgct ttatttagga aatgccttat ttagtttatc
    10501 attgattatt atgacacttc aaacaagaca ttttatgctt ttaccattca ctcttttata
    10561 tattttattc acgtttgggc gtaacatggg ctttaataat agcttagcca cggctattcg
    10621 agaattgcct gccgagaaaa atgccgatgc cacggccatt tttcagatga tgcagcaatt
    10681 tgctggcgct ctaggaactg ctatggcatc actgatagca aatagtcaac cagaattcac
    10741 aagcggtgtc cagtctgtct accttctctt tactattttt gctctacttg attttatctt
    10801 tttctttgct atgttttacc atttagggaa aaaaggactt gcctaagctg acttacttaa
    10861 aaaccagcct cgttctgttg aacaagactg gtttttttct tataaaatac cttctaaaag
    10921 acctttcata aagtcttctg agtggaattc tccaatatca tccacttttt caccaaatcc
    10981 gatgaacttg actggaatgt ctaattcttg acggatggct aggactacac cacctttggc
    11041 cgtgccatca attttcgtta aaatcaaacc tgttaatggt gttatttttg aaaattcctt
    11101 ggcttggcta agcgcatttt gacctgttga cgcatctaag gccaaaagcg tttcatgagg
    11161 ggcatctggt agcacgcgct taatgatacg acccattttt tccagctctg ccatgagatt
    11221 ttctttgttt tgcaggcgac cagccgtgtc aatcaggaga atatctacac cttttgccac
    11281 agctttttcc atcccgtcaa aaacaacaga cgctggatca gccttttcag ggccagtaat
    11341 gactggcaca tctactcggc gtccccattc gactagctga gccacagccc ctgctcggaa
    11401 ggtatctgct gccaccaaca tgactttttt accttcttgt ttgtaacggt aagccagctt
    11461 cccgattgaa gtcgttttac caaccccgtt aacacctaca aaaagcataa ccgtcaagcc
    11521 atcttgataa tt
//
LOCUS       DAUVUY010000058        11506 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.058, whole genome shotgun sequence.
ACCESSION   DAUVUY010000058 DAUVUY010000000
VERSION     DAUVUY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11506
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.058"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1137
                     /locus_tag="VOX58_001338"
     CDS             <1..1137
                     /locus_tag="VOX58_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HES5878565.1"
                     /translation="NLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVGDHASDY
                     SSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGAINILSA
                     TKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPKVFLEKV
                     EQIVRLIRSKGVGIFFVIQNPLDLPETVLAQLGNRIQHALRAYTPKEQKAVRVAADTF
                     RQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSEIESQQLV
                     QSSPFASKYSQSIDRESAYEKLAAKVLEDNRLAQEAIATAKREKEAKEAIKAQAATKK
                     ASRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            1259..2452
                     /locus_tag="VOX58_001339"
     CDS             1259..2452
                     /locus_tag="VOX58_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HES5878566.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPKELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVAPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            2449..2595
                     /gene="rpmG"
                     /locus_tag="VOX58_001340"
     CDS             2449..2595
                     /gene="rpmG"
                     /locus_tag="VOX58_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HES5878567.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            2641..2877
                     /gene="secG"
                     /locus_tag="VOX58_001341"
     CDS             2641..2877
                     /gene="secG"
                     /locus_tag="VOX58_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HES5878568.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            2971..5304
                     /gene="rnr"
                     /locus_tag="VOX58_001342"
     CDS             2971..5304
                     /gene="rnr"
                     /locus_tag="VOX58_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HES5878569.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINSQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILEAMRSKRGALNFDTQEAKVIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKAEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYSQPSQEKRDHFTQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGTK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            5307..5774
                     /gene="smpB"
                     /locus_tag="VOX58_001343"
     CDS             5307..5774
                     /gene="smpB"
                     /locus_tag="VOX58_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HES5878570.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            5780..6499
                     /locus_tag="VOX58_001344"
     CDS             5780..6499
                     /locus_tag="VOX58_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HES5878571.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(6616..7263)
                     /gene="pcp"
                     /locus_tag="VOX58_001345"
     CDS             complement(6616..7263)
                     /gene="pcp"
                     /locus_tag="VOX58_001345"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HES5878572.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(7313..8239)
                     /locus_tag="VOX58_001346"
     CDS             complement(7313..8239)
                     /locus_tag="VOX58_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HES5878573.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            complement(8239..8922)
                     /locus_tag="VOX58_001347"
     CDS             complement(8239..8922)
                     /locus_tag="VOX58_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HES5878574.1"
                     /translation="MEWIKLIGIVIIVLGFILKFDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            complement(9133..10059)
                     /locus_tag="VOX58_001348"
     CDS             complement(9133..10059)
                     /locus_tag="VOX58_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HES5878575.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPVMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            complement(10204..10581)
                     /locus_tag="VOX58_001349"
     CDS             complement(10204..10581)
                     /locus_tag="VOX58_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HES5878576.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            complement(10592..11257)
                     /locus_tag="VOX58_001350"
     CDS             complement(10592..11257)
                     /locus_tag="VOX58_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HES5878577.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            complement(11306..>11506)
                     /locus_tag="VOX58_001351"
     CDS             complement(11306..>11506)
                     /locus_tag="VOX58_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HES5878578.1"
                     /translation="GMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVRIEDCG
                     HVTKNGFEVFTHTPKELLYFEG"
BASE COUNT         3590 a         2125 c         2462 g         3329 t
ORIGIN      
        1 aatcttaatg atacccagac aggtgtctta aacatcgtct ttaaaatcgc cgacgagaaa
       61 ggttggcttt tgattgattt gaaagacttg caggctatct taaaagaagt tggtgaccat
      121 gcctcagact actccagcca ttacggtaat atcgccaagc aatctatcgg ggctatccaa
      181 agaagtctct tgacattaga acaagaaggg gcccaccaat tttttggaga gcctgcttta
      241 gatgtggctg atttgatgca attagatgtt gcttcgggtt atggagccat taacattttg
      301 tcagcgacta agctttttca atctcctacc ctttacacaa ccttcttact ttggctcttg
      361 tctgagttgt acgaattatt gccagaagtt ggggatttgg acaaacctaa gatggttttc
      421 ttttttgatg aagctcacct attgttcaag gacgctccta aggtcttttt ggaaaaagtt
      481 gaacagattg tgcgacttat tcgttcaaag ggagttggga ttttctttgt gattcaaaac
      541 ccgcttgatt tgccagaaac ggttttggct cagcttggaa atcgtattca gcatgccttg
      601 cgcgcttata caccgaaaga acaaaaggcg gttcgtgtcg ctgcggatac tttccgacaa
      661 aatcctgact tagatgtggc aagggtgatt acagaactag aagtcggtga ggccttgatt
      721 tctgttttga atgataaggg acaaccatcc atcgttgaac gtgcctatat catgccgcct
      781 aagagtagct ttgctgtttt gtcagagata gaaagtcagc agcttgtgca gtcttcacct
      841 tttgccagta aatatagtca gtccattgac cgtgaatctg cttatgaaaa attagctgct
      901 aaggttttag aagacaaccg actggcccaa gaagcgatag cgacagcaaa acgcgaaaaa
      961 gaagctaaag aagccatcaa ggcgcaagca gctaccaaaa aagctagtcg ccgttctgtt
     1021 gggcgtccac gaaaaacagt cgtcgaaaaa gcaacagatg cgtttattag tacgacggtt
     1081 agaacaattg ggcgagaact ggtcagaggt ttactgggta gcttacgtaa acgatgaagg
     1141 ctatcagatg agacttagca ctattatggg acactgtttt tggcagaaat gtgttataat
     1201 aatcaaaggc ccgatggaaa gaagatgatg atctattagt gataaagcgg taacgacagt
     1261 gaactggcgg caaaatctaa aagtggcttg gcttggtaac ttctttactg gggcaagctt
     1321 ttcacttgtt atgcctttta tggctctgta tgtggaaaat ttaggtactc cgaaagaatt
     1381 agtagaatac tatgctggtc tggcggttgc tgtgacggca cttgcttcag ctttatttgc
     1441 tcctgtttgg gggaagttgg ctgatcgtta tggccgaaaa cctatgatgc tgcgagcaag
     1501 ttttgtcatg acctttacca tgggtggttt agctattatt cctaatgtgt tttggttact
     1561 cattttgaga ttattaacag gtgtctttgc gggctatgtg ccaaatgcca ctgccttgat
     1621 tgccagccaa gccccaaaag aggagtcagg ttatgcttta ggcacattag caacaggagt
     1681 gacagcaggc gctttaattg gtcctttact aggggggatt ttggcagagc tattaggaat
     1741 tcgacaagtt ttcttattag ttggtgtgat attattttta tgttctttaa tgacagctgt
     1801 ttatgttaaa gaagagttta agccgattag gcgctttgag atgataccta caaaagtcat
     1861 tttaaaacag gtaaaaagtc cccagattat gcttggtttg tttgtgacta gtatgattat
     1921 tcaaatttca gctcaatcag tggcccccat tttatcattg tatattcgtc atcttggtca
     1981 aactcacaac ttaatgttta cttcaggcct ggtagtttcg gccatggggt tttctagctt
     2041 atttagcagt tcttacttag gaaaattagg agatcgtttt ggcaatcatc ggttattgtt
     2101 agccgctttg tgttatagct ttatcatgta ttttagcagt gccttagcgc agaccagttt
     2161 tcaattagga gtactacgct ttgcttatgg gtttggtgtg ggcgctttga tgccaagtat
     2221 taactcttta ctaactaagt taacaccaaa agaaggtatt tctagagtat ttgcctataa
     2281 tcaaatgttt agtaaccttg gtcaagtcat tggccctttt atcggatcaa atgtggctgt
     2341 agtgttaggc taccgttctg ttttttatgt gactagtttg attgtgtttg ttaatttaat
     2401 atggagtttg attatatttc gaaaatatat taaagttaag gatattgtgt gagagtaaaa
     2461 attaatttag aatgtagtga gtgtggcagt aacaattatc taaccagtaa aaataaaagc
     2521 agtcatccgg aaaaaataaa agttcccaaa tactgcccca aggagagaaa agtgaccctg
     2581 catgttgaaa cttgataaga attgtgttag aatggtcatg acaatagtgg aggaaataac
     2641 atgtacaact tactacttac gatattgcta gtactgtcgg tattgcttgt gattgctatt
     2701 tttatgcaac ctcaaaaaaa cccaagtagc aatgtttttg atagcagcgg ttcagaagcc
     2761 ttgtttgaac gtacaaaagc tcgtggcttt gaagcattca tgcaacgatt cacagcagtt
     2821 ctagtatttt tttggctagc gattgcactt gtgcttgcca ttttatcaag taaataagga
     2881 gtgagcttga cagagttagt ctagctcatt tttttatcag cgaaacggat tgatatcctc
     2941 caacaaacaa aacagaaata gaaattaggt atgatgaaag aaagaattat gacttacctc
     3001 aaggagcatg gcaagtccaa tgtgaacgac ttggctgcgg ccttggagat ggctggagct
     3061 aagcattttc ccagccttat taaaaccatt tccaaaatgg aaagtcagag cctgttacgg
     3121 ttttcagatg atggcagtct ggctcttaga aaagagcgtg aaaagaaaaa agaacctact
     3181 gttcagggtg tttttcgtgc caataaagca ggctttggtt tcttacatgt ggatgagaat
     3241 gaagatgaca tgtttatcgg tcgtaatgat gttgggtatg ctattgatgg tgatactgtc
     3301 gaagtagtgg ttaaaaagcc agcagaccgt ctcaaaggca cagcggctga agctaaggtc
     3361 gttgctattg ttgaccgttc tttaaaaaca gctgttggaa cctttatttt agatgatgac
     3421 aaaccaaaat atgctggtta tatcaggtcc aaaaaccaaa agattcaaca aaaaatctat
     3481 atcaaaaaag aacctgttgt gcttaaaggc actgaaatca tcaaggttga tattgataaa
     3541 tacccgacac gcgggcacga ttattttgta gccagtgtgc gtgatatcgt aggccatcaa
     3601 ggagacgttg gaattgatgt cctagaagtc cttgaatcga tggatatcgt gtcagaattt
     3661 ccagcagaag ttttagctga ggccaatgct atttcagaag caccaactgc taaagattta
     3721 ataggccgtg tggatttgcg tcaagaaacc accattacta ttgatggtgc ggacgccaaa
     3781 gacttagatg atgctatcca tattaagcta ttggataacg gtaactatga acttggggta
     3841 catattgcgg atgtctcata ttatgtgaca gaaggatctg cccttgataa ggaagctatc
     3901 gcccgtggca cttctgtcta tgtcaccgat cgtgtggttc cgatgttacc agagcgtttg
     3961 tcaaatggca tctgctcttt gaaccctaac gttgatcgtt tgacccagtc agccttgatg
     4021 gaaattaatt ctcaagggca tgtggtcaat tatcaaatct gtcaatcagt gattaagaca
     4081 acttaccgaa tgacgtatag tacggtcaat gacatgattg caggagatga agaagcgctc
     4141 caagaattcg ctagcattgc tgatgatgtg accctcatgg tggctttgca taggattttg
     4201 gaagctatgc ggtcaaaacg aggcgccttg aattttgata cgcaagaagc taaagttatt
     4261 gttaatgaca aaggaatgcc ggttgatgtg gtgcttcgtc aaaggggaat tgcagagcgg
     4321 atgattgagt cctttatgct agcagccaat gagtgtgtgg cagaacattt tgcaaaagct
     4381 aagttaccgt ttatctaccg tattcacgaa gaaccaaagg ccgaaaaact tcaacaattt
     4441 attgattacg ctagcacctt tggtattcac attcaaggaa cagccaacaa gatttctcag
     4501 gaagccttgc aagcctttat ggcaaaagtc gaaggtcaac caggagcaga agtgcttaac
     4561 atgatgctct tacgctctat gcaacaggca agatattctg aacataatca tgggcattac
     4621 ggtttggccg cagaatatta cactcacttt accagtccga ttcgtcgtta ccctgacttg
     4681 ttggttcacc gcatggttcg tgagtacagt caacctagtc aagaaaagcg cgaccatttt
     4741 actcagatca ttccagaatt agcaacatct tcttcacagt tagagcgtcg tgctattgat
     4801 gctgaacgcg tggttgaagc catgaaaaaa gctgagtata tggcagagta tgtgggtgaa
     4861 gaatttgacg gtatcgtggc cagtgtcgtt aaatttggtt tttttgtaga attgccaaat
     4921 acgattgaag gtttggttca tatcactagc ttaccggaat actatcactt taatgaaaga
     4981 acgctaagcc ttcaaggtga aaaatcaggt aaagtcttta aagttggtca acctattcgt
     5041 gtgaaattgg ttaaggctga caaggaaact ggtgacattg actttgagta tttacctagc
     5101 gactttgatg tggttgaaaa aatcaagatg tcggataaag ctagtcgacg agatcgccgt
     5161 aaatcctcaa aatcatctaa agggacaaag aaaaaagaac ctaaagaagt agccaaggca
     5221 aaaaccaagg gtaaaactaa aaaagggtct aaaaaaccat tttacaaaga acaagcaaaa
     5281 aagaagagtc gtaaaaggag ttaactatgg caaaaggtga aggtcatata ctggcgcaaa
     5341 ataaaaaggc tagacatgat tatcacattg ttgaaaccgt tgaagcaggg attgtcttaa
     5401 cgggaactga gattaaaagt gttcgtgcag ctcgtattca actgaaagat ggctttgccc
     5461 aaattaagaa tggggaagcg tggttggtca acgtgcatat tgcacctttt gaacaaggta
     5521 atatttggaa cgctgatcct gaacggaccc gcaaactgtt gctcaaaaaa cgtgagatta
     5581 cgcatctagc aaatgaactt aaaggatctg gtatgacttt ggttcctctc aaagtttacc
     5641 tcaaggatgg ctttgctaaa gttctgattg gtctagctaa agggaaacac gagtatgaca
     5701 aacgcgaaac cattaaacgc cgtgatcagg aaagagatat taaaaaacaa atgaaacact
     5761 acaatgcaag gtaaggtgaa tgagtattat gataactaaa ggacaagttc gtttgttacg
     5821 aacttattct tatgatagca acttatacac gcagggatta gaacaactca acaacaatca
     5881 tatattactt agtgccggtc gttatggttt ctcaaaagta ggggtgtatg accttactca
     5941 agagatcttt tcagaaaaaa tagcttttcc agatacagtt tttgcagaag ggctaacagt
     6001 tgttgaggat tatttttggt tactaaccta taaagagggt gtagcctaca agtttgataa
     6061 ggctacctgc aattgtttag gggcctatcc atttgaagga gatggctggg gattagccta
     6121 tgataaggaa aaccagtgtc tttggatgac tagtggaaat gcttttttgc aaaagcgaga
     6181 ccccaaagat tttgctttat tggataccgt actagttgct atagaaagtg tccccatctc
     6241 aatgcttaac gaattagagt atgtagatgg ttatctctat gccaatattt ggcaaaccaa
     6301 tactattgtc aaactccaac ctgattctgg aaaggtggta gccacctatg atattagtcc
     6361 tttgttaaaa gccttaaatc ttgataagtc ccattaccct gaccttaatg ttttgaatgg
     6421 gattgctcat ttagatcaac aacgtttctt aattactggc aaactatacc cgctgatgtt
     6481 agaagtagta ttagattaag atgacaattg agaaagaccg atgaaaagtt tccctaggtc
     6541 tttcattttt agcacaaaaa aaacagagtt agttcatatc tctgtttttt taaaataggt
     6601 ttagtagcgt cacggtcagt gagtagcgcc tcctacacgt tttaaatcgg aacgatcttt
     6661 gaaatcgaca atggcaaaaa tagcagcctc aattcctctt gtaatatcat cgaggttcat
     6721 ggcagctgta ttaggtttat caacaacctg ttccatcata aagggaatat gcataaaccc
     6781 agctttggca tttggacaat atttatccac taagtaaagg gcttgataca tcaaatgatt
     6841 gcaaacaaag gtaccagctg tattagaaac agaagcagga agcccagcct gatgaatggc
     6901 agcaaccatc gctttgattg gcaaggttga aaaataagct gctttaccat ctgcacgaat
     6961 aggtgtatca ataggctgat tcccttcgtt atcaggaatg cgagcatcgt cttgattaat
     7021 ggcaacgcgt tctggcgtta gtccagtccg gccaccagct tgcccaatac aaaggactgc
     7081 atcaggttga aagctttcga tatgctgctg gagcacatcg gcagattttt gaaaaaccgt
     7141 tggaacttca atacatttga tttctgctcc atgaatggtt gctggcaatt tcttgatagc
     7201 ttcaagggca ggattaatag cttcgccgcc aaagggatca aagcctgtta caagaatttt
     7261 cataataatg tcctttcaat gaataactga cgctactaac ttagcaaaaa agttaataat
     7321 aagcaagtaa atacattaag gcaatgtgac taataattaa aacaagggcg ataggagctt
     7381 gctttttgat aactgcattt cgatctttga tctccatcaa agcaacaggc aaggcattaa
     7441 agtttgctgc cattggtgtt aataaggtac cacaaaaacc agcagtcata gctaaagcac
     7501 cggcaataat agggtcagct cctaaagcaa aaacaaaagg aacaccgata ccggttgtaa
     7561 taacggtaaa ggcagcaaaa gcgtttccca taatcatagt aaaaataacc atgccaagca
     7621 cataagcgag aacaccaaag aagcggctat cagcaggtac aatctcccga atgagagaag
     7681 caatcacatc accaacacca gcagctgcaa aaatagctcc taaagctcct aagagctgtg
     7741 gcaaaatacc gcttgtggac acttgttggt tcatccgatt attttcagct aaaagagcag
     7801 atggtttttg cttagtgatt attaaaatag caatagtggc taagatacca gcaatcccaa
     7861 tggcactttt actgaaatca ggaaggatca aggcaagaag tagagaaatc atagccatta
     7921 acataactgg taagaaaatc cagttaccaa tgcggtgagc actttcttca gctttttgtt
     7981 cattaaaggc tggtaatgtt ccaatacgaa cttgcttaaa caaggttaaa agagctagga
     8041 caatcacgat aaaaccgata actttattag gcaaaaaggc accgccaacg aaggtaacac
     8101 ccagcaatcc ccagaacaga gctgtcccaa aatagacagg gttggtttta tctttaaagg
     8161 tgcagtaggc tgtgtgaaac agttgcaagc cgataaggat atatatcgtt tctaaaacaa
     8221 ggtttgcaat gttagccatt agttatctcc tttctgatgt tgacgttcca tttttttctc
     8281 aaagaggtaa ttgtaaatag caactataat aatagagata atagcaatta aaatagaaga
     8341 aaatgcaatt ttagcttggt taccatcata gccaagctgt tctaaagttc cagcgataag
     8401 taggacacct cctgcgccaa caaaggtatt ttgggcaaag aaattgccaa agttttcatt
     8461 ggcagctgcc atagctttga tgtcatcttt ttctgcatcg gtcaactctt ctccaatgtt
     8521 ggctttagca gcagcttctc ccataggttg aatcaagggg cgaacgaact gtggatgtcc
     8581 ccctagtcga atcgaaaaga gacctgctaa ttctcgaata ataaggtaga gagtcaataa
     8641 acggccgacg gtaagggctt gaacacgttg aatcaactga gtagctcggt gtttcagccc
     8701 ataagtttct gacaagccga taagcggtaa agtgatgaag aaaatggtta gcaaacgttg
     8761 attggtaaat tcttttccta aaatatctaa aaaatcaata aacgaaattc ctgaaaccag
     8821 cgcggtaaca agtcctgcaa caactacggt agcgatagca tcaaatttta aaataaaccc
     8881 taatacgata atcacaatac cgattaattt aatccactcc ataaagaact cctttttcaa
     8941 tagtatgacg attataacat tagtcgctag gatttgcaaa aaggctcaac ctaatagttc
     9001 cttttctact taaagtgtta aggaaagtga tactattttg gaaaggtcat tatcatttgg
     9061 tataacgggc ttaaaaagaa agcttcgcgc acagaaggag taggaaacga ttgatgagaa
     9121 aaatgtttta atttaatgtt tttctttgat aatatagaga ggcctttttt tagtttcaag
     9181 gaatattttt gaaatatatt ttccaataat acccatgcaa aagagctgaa tgcctcccat
     9241 gaataaaata atagagacag tgctggccca accagagaca ggatcaccaa agagtatctt
     9301 tctgataatg ataaataaga tggcgaaaat agaaatcaaa aagctgaaag tccctgtcca
     9361 ggtagcaatg gttaacggca tttctgaaaa gttgataaag ccatctaaag aatatcggag
     9421 caactcccaa aaatgccaac gactttttcc atatttgcgt ttttgatttt caaaactaag
     9481 ataggtgata cgataaccaa cccacgaaaa gatccctttt gaaaaacgat taacctctcc
     9541 caactcaaga atgctatcaa cgacttgtct ggtcattaac cgataatccc gaactccatt
     9601 gaccatttca gtatctgata agtgtttgat cagtccataa aatagattcg aacacattga
     9661 acgaattagt ggttctcctt gtctattttg gcgcctggtg ccaacgatgt cataaccctc
     9721 ttttagctta gcgtacataa ctggcaaaag ttcgggtgga tcttgcagat ccacatccat
     9781 cacagtaatg taattgcctt tggcttcttt tagtccagct aagagtccag cttccttgcc
     9841 aaaatggcgt gaaaaagaca gataatgcac atttggaaag cgagcagcta gctcacgcaa
     9901 aatacccaag gtgttatcct tagaaccatc atcaataaaa atgtattcaa aagcaagttg
     9961 gttggtcatg gacgtttcta gttgatgcat ttcttcaaaa taagggagaa tgtttgcttc
    10021 ttcattaaag caggggacaa tgatagatag tagtgtcata gacttttctt tcaaatggaa
    10081 ttttagggca attgtaacac aaaagctgag atatttcaaa actctgttaa taatgagatt
    10141 gtcccaatcg tcaggttaaa aaataaaaca acagaagctt taaagcttct gttgttaata
    10201 gtattagttg tttaaatcaa tgacttcaat tttgtagcca tctggatcct gaatgaaata
    10261 atagcgggca gatttgtcag ccagttcttt gatatcggta actggaaaac ctgcttgtcg
    10321 atgtttttta tggtctgctt caaaatgttc agagccaagg gcaatatgac catagccatt
    10381 tcctaagtcg tagtcgccat gaccataatt gtaggttaat tctaattcat aactttctcc
    10441 ttctaaagcc agataaacaa gagtaaattg actatctgga aaatctttac gatagttttc
    10501 cttaaaaggg aaggcgcttg tgtaaaaagc aaccgactgg tcaaggtctt tgacgcggat
    10561 acaggtatgt aatgctttca ttttaatctc cttatttaac gactgaaatc acttcttctt
    10621 taggcttacg aacttgagca tgtttgggat ctcgcaggcg atagcctagg gataacatgc
    10681 tagcaatgcc ttctttttct aaatcaatca cattatgctt agctaggata tgattgacct
    10741 tatcataatg aaagccttca atagggcaag tatcaatgcc caaaagagca gctgtcatca
    10801 tcatattacc aagagcgata taggtttgtt tagctgtcca atcaaagagt gcccgaggat
    10861 tatctgccat atccatgtcc tctttttgga aagattcata gagttttagg cggctgttga
    10921 gaccatcacc ttctttgata ccacgccgta aaaggctatt tttgatagca gggctatcgt
    10981 atcttgcatg tttttcggct attaaaagaa taaagtgact agctgtctcc agttgatact
    11041 gggcccccca ggcaaagggc ttgatttctt ctttaatagg cttgttgtct aaaacgacaa
    11101 agcgccagcc ttctaagcca acagaagaag gacttaacca agcagcatca aggataaggg
    11161 ctaaatcctc atcagaaatc ttttcttcct tataaacacg aacggctgtt ctaaagtgta
    11221 gtgcttgctg tatttggtga tgaatggttt gatccatacg aataccttcc tttcgcgagg
    11281 gtttgtttaa ccagatgata ggggcttagc cttcaaaata aagcaactct tttggagtat
    11341 gggtaaagac ttcaaagcca tttttagtga catggccaca gtcttcgatg cgcacaccga
    11401 cctttcctgg aatgtaaata ccaggttcaa cagagaagca catgccttct tcaaggacca
    11461 agtcattgcc agccatgatg gagggaaatt cgtggacatc catgcc
//
LOCUS       DAUVUY010000059        11430 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.059, whole genome shotgun sequence.
ACCESSION   DAUVUY010000059 DAUVUY010000000
VERSION     DAUVUY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.059"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..672)
                     /locus_tag="VOX58_001352"
     CDS             complement(<1..672)
                     /locus_tag="VOX58_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HES5878579.1"
                     /translation="MTRSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYGGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPESREAALKAADKLIQRYQDKGGFIQ
                     AWGVLGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYF"
     gene            complement(672..1109)
                     /locus_tag="VOX58_001353"
     CDS             complement(672..1109)
                     /locus_tag="VOX58_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HES5878580.1"
                     /translation="MIAIIVMGHGHFASGIVSSLELIAGPQEKVTAIDFTTEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGTTDDELGGI"
     gene            1444..2238
                     /locus_tag="VOX58_001354"
     CDS             1444..2238
                     /locus_tag="VOX58_001354"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HES5878581.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGVAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            2263..2904
                     /locus_tag="VOX58_001355"
     CDS             2263..2904
                     /locus_tag="VOX58_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HES5878582.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVAYLKSVLEQ"
     gene            2933..3934
                     /locus_tag="VOX58_001356"
     CDS             2933..3934
                     /locus_tag="VOX58_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HES5878583.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEANIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMSLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGISHFHFSGISLALGKKTQDLIEILAREARKRQV
                     CVSFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADTTMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLHNQRDVQR"
     gene            3939..4574
                     /locus_tag="VOX58_001357"
     CDS             3939..4574
                     /locus_tag="VOX58_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HES5878584.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHGYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGISFIRAIKSPLPQVEVMV
                     TGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            4870..5520
                     /locus_tag="VOX58_001358"
     CDS             4870..5520
                     /locus_tag="VOX58_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HES5878585.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITTDYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSEKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            6217..7341
                     /gene="queG"
                     /locus_tag="VOX58_001359"
     CDS             6217..7341
                     /gene="queG"
                     /locus_tag="VOX58_001359"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HES5878586.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVMEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNSPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGGWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEMLAFMSHLTLKDEDSQKELELIRRKWQV"
     gene            7410..8508
                     /gene="prfB"
                     /locus_tag="VOX58_001360"
     CDS             join(7410..7481,7483..8508)
                     /gene="prfB"
                     /locus_tag="VOX58_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HES5878587.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            8527..9219
                     /gene="ftsE"
                     /locus_tag="VOX58_001361"
     CDS             8527..9219
                     /gene="ftsE"
                     /locus_tag="VOX58_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HES5878588.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            9212..10141
                     /locus_tag="VOX58_001362"
     CDS             9212..10141
                     /locus_tag="VOX58_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878589.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(10451..11149)
                     /locus_tag="VOX58_001363"
     CDS             complement(10451..11149)
                     /locus_tag="VOX58_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HES5878590.1"
                     /translation="MPFIFRHSFFNKALIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIITVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            11317..>11430
                     /locus_tag="VOX58_001364"
     CDS             11317..>11430
                     /locus_tag="VOX58_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HES5878591.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGILDYNEET"
BASE COUNT         3527 a         1912 c         2492 g         3499 t
ORIGIN      
        1 aaagtagaag gtgtggtaag cagaggcgtc atctcggatg acatgattgg ctgaggcgta
       61 gaaatgattg atcgccatgt cacggtaacg gttgtctcca gtttcttggc tagcaaaaaa
      121 gagtaattgg atgtttagga gacaatcaat gattaaacgg taatcttctt ttttacccag
      181 caccccccag gcctgaataa agccgccttt gtcttgatag cgttggatta atttgtcggc
      241 tgctttcaaa gcagcttctc tagactcagg tgtttttaaa agtttccact cagccatgca
      301 agaaggggtg taaagaaagc ccaaatcatg gtgatctagc tcaatatctc ttgttactcg
      361 atctaggaaa gaaaggtcat ttgcttgagc cagagcttta atggcatcat ccccaccgta
      421 ttcgtaagct aaccaaaggc aacctgtcca aaaggcattg gtccattccg tgttgtccat
      481 aatggcgtat tggttatctt tggtagctgg cgttggaaag tcttctttaa aatagtccat
      541 attaagccga acttgtttga gggctaagtc aagggcctga gtaatgtctt cttggcttag
      601 aaagtcttct ttggtaaatc gttcaggctg cttgataggt tcaagagcaa tggtttttaa
      661 agatcgtgtc attaaatgcc tcctaactca tcgtctgtcg taccgtcttc ttgctgagaa
      721 agagttttcc agtcaacaat gccttcttta gaacatgtta taagtcctga tactaagtcg
      781 tcaaaagaag ctgctgtttg tcttgcaaaa ctagcttcaa tcagcatggc taagttaagg
      841 ccagaaagaa cattacacgt cgtgtttgga agagattcca ttaaggttgc agcgacttta
      901 aaaggggtac ctcctagaag gtcacaaaga actaaggttt cctcttctgg gattaaagca
      961 cgagataatt ggtcctgcac atcagcggcg gtcatctcag tagtaaaatc aattgcagtt
     1021 accttctctt gtggtccggc aattaactct agggatgaga caatcccact cgcaaaatgt
     1081 ccatgtccca taacgataat agcaatcata aataaatccc ctttggttta ccggtttact
     1141 caaaaatatt gtagcgcttt caaaattctt tgtcaatact ttttttaaaa gatttttgaa
     1201 aaagaaaaag tgaacggttg tgctaaaaaa gaatcaaaaa tggtcttttt tgtcactatt
     1261 taatgtagaa aaatcttaat agctattaag tataggacaa gactaaacaa gaatgacagt
     1321 ttggataaga gcactctttt aaattttgaa ccgttttcat taaaatgatt gacaaaacta
     1381 gtttactttg ttatagtctt ggtaaacagg tttacttaat ctaataacaa aaaggagaat
     1441 cctatggaaa atatgttttc gttacaaggt aagattgcct tgattaccgg agcatcttac
     1501 ggtattggtt ttgaaattgc caaagcctat gctcaagcgg gtgcaactat tgtgtttaat
     1561 gacatcaaac aagagttagt tgataagggg gtggctgcct atcgagagtt agggatagaa
     1621 gcgcatggct atgtttgtga cgtgactgat gaagctggta tccaacagat ggttagtcaa
     1681 attgaagatg aagtaggtgt tattgatatt ctggtcaaca atgcaggtat tattaggcgt
     1741 acgccgatgc ttgaaatggc cgcagaagat tttcgtcaag ttattgatat tgatttaaat
     1801 gctcctttta ttgtgtcaaa agctgtatta ccatcaatga ttgcaaaggg acatggtaaa
     1861 attataaata tctgttctat gatgagcgaa ttggggcgtg aaacagttag tgcctacgcg
     1921 gcggctaaag gtggcttgaa aatgttaacc aaaaatatag cctctgagtt tggagaagcc
     1981 aacattcaat gtaatgggat tggtcctggc tacattgcga cgccccaaac agctccgttg
     2041 agagagcgac aagcagatgg tagtcgtcat ccttttgatc aatttatcat tgctaaaaca
     2101 ccagcagcac gttgggggac aacagaagac ttagctggtc cagctgtttt tctagctagc
     2161 gatgccagta attttgtcaa cggccatatt ctttatgtcg acggtggcat tttagcttat
     2221 attggaaaac aaccttaatc acacactaga aagagatttt atatgaaaat tgcattaatt
     2281 aatgaaaata gccaagcggc taaaaatggt attatttacg atgcccttac ctcagtgaca
     2341 gataaacacg gctaccaagt ctttaactat ggcatgtatg gcacagaagg tgaaagccag
     2401 ttgacctatg ttcaaaatgg actcttagct tctattttat tgaccactaa ggcggccgat
     2461 tttgtggtga ctggttgtgg gacaggtgtg ggagctatgt tggccttgaa cagcttccca
     2521 ggggttacct gtggttttgc gagtgagcca acagaagcat atctcttttc tcaaatcaat
     2581 ggtggtaatg ccttgtccat tccttttgcc aaaggttttg gttggggagc agagttgaat
     2641 ttgaccttaa tatttgaacg cctctttgca gaaccaatgg gtggcggtta ccctaaagag
     2701 cgagctattc ctgaacagcg gaatgcacgt attttaagtg atctgaaaaa aattacttac
     2761 agagatttgt tggcaattgt caaagacatc gaccaagatt tcctcaaaga aacgatttct
     2821 ggcgctcatt ttaaagagta cttctttgcc aatgcggagc cgtcagaatt agtcgcttat
     2881 ttgaaatcag tacttgaaca gtaattccat tttgacgagg aaaggaggtc acatgtctaa
     2941 gttattattg gttggtgaac cgctgattcg cgttagcccc aatcagtttc agcctttgac
     3001 caatgcctgc gacgctcagc tttttttcgg cggttcagaa gctaatattg caagaactct
     3061 aggcggtttt ggcttagaag caaggctatt tacggcccta ccagataatc ctgttggtca
     3121 cgcttttcat cagtttttga agcaaagcgg tgtggacatg tccttgacgg cttggcaagg
     3181 tcatcgcgtt gggctatatt accttgaaaa tggctttggc tgtcgggcta gtcaggttta
     3241 ttatgaccgc tgtggctcta gcttttcggc cttagataag gatagtcttg atttagctgc
     3301 catttttgaa ggcattagtc actttcattt tagtgggatc agtttggcgc tgggcaaaaa
     3361 aactcaggat ctgattgaga tactagcgag agaagctagg aaaaggcaag tttgtgtttc
     3421 ttttgatctt aactttagat ctagtatgat tgcggtagct gatgctaaac gactcttttc
     3481 tcactttgct caatacgcgg atattatttt tggcatggaa cctttgctgt tagatagtga
     3541 cgattttgac atgtttgata ggaataaggc agatactacg atgattagag aacgtctggc
     3601 aggactttat cagcgctatc agttacaagc tatttaccat acggagcgtt caaatgatgc
     3661 ccaaggcagt aatcgtttca aggcttatgc ctatgatggg cagttttacg aatcctgtga
     3721 agtgacgacc ccagtcttgc aacgggttgg aagtggagat gcttttgtgg cgggtttact
     3781 ttatcagttg ttagaaggta acgaaaagca gcgcaacctt gattttgctg tggcaacagc
     3841 tagcctaaaa tgtacagtag ctgaagatca gctctatcac agcgtccaac aagtagaagc
     3901 agtcttacat aatcaacggg atgtccagag gtgatgatat gggaaaaata gaaatcttaa
     3961 ccaaacttaa ggctaatcgg cttgtcctgg ttgtacgagg agagtctagt gaggaagctt
     4021 tagcttgcag cctggccagt attgaaggtg gcattaagac aattgaagtc acctatacta
     4081 acccctttgc tagtgaggtt atcggtcaat tagctgaacg cttcaaagaa gatcctgagg
     4141 tattgatagg tgctgggaca gttttggatg atgtcactgc ccgccaagct attttagcag
     4201 gagcacagtt tattgttggt cctaatttca atcgagcagt ggctttgatt tgtcatggtt
     4261 atagcatacc ttatttacca ggttgtatga cggttaatga agtggttaca gcattagaat
     4321 caggggttga tatggtcaaa atttttcctg gttctactgt agggatatcc tttattcggg
     4381 caatcaaatc tcctttgcca caagtagagg ttatggttac aggcggagtt tcttcagata
     4441 atctcaaaga ctggctagct gctggagtag atgttctagg cataggaggc gagtttaatc
     4501 agttagcgag ccaaaaacaa tataacctaa tcactaagaa agccgctcat tatatcaaaa
     4561 gtttgcgaca ataatctaaa aaacgttttt accttggctt tagtgctagt aaaaacgttt
     4621 ttttatcatt cattcaatta atatgcttct gctcatgttt ttccagatag tatagatata
     4681 agagcagcta atttaacaag atgagcctgt tgtttgatat ataggatata aactttttat
     4741 gatcaaacaa aagtaatacc ttttgaattc gttagtattg atctgatcta aagtgacatc
     4801 acttagaatg gactagctgt acagctttta cagcaactta tgctaaaata gaaaaaagga
     4861 gaaatgttga tgattaaagg aattattttt gatatggacg gtgttttatt tgatacagaa
     4921 cctttttatc tgaggcgacg agaagatttt tttaagacaa agggaattcc cattgatcat
     4981 ttgaactcta aagattttat tgggggcaat cttcaagaat tatggaaaga gttgttaggt
     5041 aaaaataggg atgatgctat cgttaaggca attacaactg actatgacgc ttacaaacaa
     5101 gcgcataagc ctccttatca aaaactgttg attacagaag tgaactcttg tcttgaacag
     5161 ttgaaaaaac aaggtattaa actggctgtg gcatcaaact cgaagcgtca ggatgttttg
     5221 ttggcgttgg agacaacgca aataaaagat tattttgaaa taatcctagc gcgtgaagat
     5281 gtttctagag gcaaacctta tccagatatc tataataaag cagtacaaaa actaggatta
     5341 caaaaaaaac aactgcttgt agtagaagac agcgaaaaag gcattgctgc cgccaaagcg
     5401 gccaatctga cagtttttgc cattaccgac taccgatatg gcattgatca gagtcaagct
     5461 gatcacaaga tagatcattt aggacaactg tgtgtaaaaa tcggttgttt tggatcgtga
     5521 agtaacgcaa ctaactgatc attgatgata cttcaagaca cttaagaata cgtctaggac
     5581 tttctacaga ccaataacaa gaatacgttt atgtgttttt actgtttttt agctacctat
     5641 ttacaatatg acactgaaaa gagtactatg tttaagttat tatcaaaaaa aaccgtcgca
     5701 taagatcacc tagaaaagga ttatgatttt tgtgatttgg atgcgatcat tagtcgttga
     5761 ccatctttat gtggcaggag atatgtttaa ccgtggtcat tatttggatt tgtttttaga
     5821 ttggctagtg agtaaatata gaaggatcat aatactgctt tgacaaaggc agtatctgag
     5881 tccatgagtt gcacgggtaa tgtgactcgg ttttggttag gtataataat atttggaagt
     5941 gttatgctgc ttgtcttcgt aggtccattg cttatagtca gacttacttt agagctgagc
     6001 tgactgtcta gacaagacgt gatggagatg tgatttctga ccaagtagag gtaaccagaa
     6061 aagggacttt tacgatgtaa ttgaatttac ttgttgcatt cttgtgcatg ttaagagaga
     6121 aaacccagat aaatcaggaa gtaaaatggt tatgtttgat tagaattgtc cttctttttc
     6181 cttgaagtct taacttattt tgattatgat agtagtatga ctattaaggc agaaattaag
     6241 gcactcgcca aagaaattgg tatctctaaa attggtttta cgacggcaga taactttgat
     6301 tatttggaaa agtcattgcg tgcttctgtg gaagaggggc gcaattcagg atttgaacat
     6361 aaagtcatgg aggatcgtat ctaccctgaa aggcttttag aatcggctaa aacgattatt
     6421 tcaattggcg ttgcttatcc tcacaaactt cctcagcaac ctcaaaaaac accttataaa
     6481 cgtggaaaaa ttacacctag ttcgtggggg cttgattatc attatgtggt cggcgaaaag
     6541 ttagacagac tatcaaaagg cattgaagag ctgtgtcgag attttccttt gcaacaaaaa
     6601 gcaatggtcg atactggagc tttagttgat actgcagttg cccaaagagc gggtattggt
     6661 tttataggaa aaaatggttt agttatttct aaagaatatg gctcttatat gtttttggga
     6721 gaattaatta ccaatttaga gatcgagcca gacaagcctg tcgattatga ttgtggtgac
     6781 tgtcgtcgtt gtctagatgc ctgtccaact tcttgtttaa ttggtgatgg ctcaatgaat
     6841 gccaagcgtt gtctatcatt tcaaacccaa gataagggaa tgatggacat agagtttcga
     6901 aaaaagatta aaacagttat ctatggttgt gatatttgcc aaatttgctg tccttataac
     6961 aagggtataa acaattcacc agctacagag attgatcctg agttggctca accagaatta
     7021 ataccatttt tgagtttatc aaatggacaa tttaaagaaa aatttggtat gattgctgga
     7081 ggttggcgag gtaaaaatat tttacagcgt aatgccatta ttgcgctagc aaatgctcat
     7141 gataaaaccg cagtggtcaa attaatagag attattgata aaaataataa ccctattcat
     7201 actgcaacag ctatatgggc attgggagaa attgtcaaaa aaccaaacga tgagatgctg
     7261 gcgtttatga gtcatttaac cttaaaagat gaagatagcc aaaaagagtt ggaattaatc
     7321 cgtcgtaaat ggcaagttta agccttttat ggtattttta agaaccttat gataaaataa
     7381 aacagattaa caaagattgg agaaatctca tggaagtggc tgaaattcgc caaaaaatag
     7441 tagaaaataa agagaagttg actagcttca ggaggtctct ttgacttaga tcgtttggaa
     7501 gaggagattg ctcttcttga aaatcacatg actgagccag acttttggaa tgacaatatt
     7561 gcagcgcaaa agacatctca agaactaaat gagttaaaag gtaagtatga tactttccat
     7621 aatatgcaag aattatctga cgaaacagaa ctcttacttg agatgcttga cgaagatgat
     7681 tctttgaaag aagaattaga agaaaatcta atgcagctag ataagataat gggtgcttat
     7741 gaaatgacgc ttctcttatc tgaaccctat gaccacaata atgctatctt agaaatccat
     7801 cctggttcag gaggaactga agcgcaagat tggggagatt tactcttacg aatgtatacc
     7861 cgttttggca atgctaatgg tttcaaagta gaagtattgg attatcaagc aggagatgaa
     7921 gctggtatta aatcggttac cctctctttt gaaggcccca atgcttatgg tttgttgaaa
     7981 tcagaaatgg gagttcatcg cctcgttcgt atatcacctt ttgattcagc taaacgtcgt
     8041 catacttcat ttgcttcagt tgaagtgatg ccagagttag atgatacgat tgaagtagaa
     8101 gtacgtgatg atgacattaa aatggatact ttccgttccg gaggagctgg tggtcagaac
     8161 gtcaataaag tatcaacagg tgttcgtttg acgcatattc caactggaat tgtggtatcg
     8221 tctaccgttg accgtactca atatgggaac cgcgatcgcg ctatgaagat gttgcaggct
     8281 aagctatatc agttagaaca agaaaagaaa gcacaagaag tagatgctct caagggtgat
     8341 aaaaaagaaa tcacttgggg aagtcagatt cgttcttatg ttttcacccc ttatacgatg
     8401 gtgaaggatc atcgtactaa ctttgagctt gctcaggtag ataaggttat ggatggagaa
     8461 atcaatggct ttattgatgc ctaccttaag tggcgcattg aagactaaaa ataataaagg
     8521 agaaacatgg cactaataga aatgtctggt gttactaaaa aataccgccg ctcaacgacc
     8581 gccctcagag acgtcaatgt ttctgttaat caaggtgagt ttgtttatct cgtaggccca
     8641 tcaggtgctg gtaaatcaac ttttatcaaa ctcctttacc gtgaagaaca actgacaact
     8701 ggtaagctat atgtgggtga attcaattta actaaactaa aagcacgtga tgttccgatt
     8761 cttcgtcgtc acatcggagt agtttttcaa gattacaaac tgctaccaag aaaaacagtc
     8821 tttgaaaatg tagcttacgc catggaagtt attggtgaaa agcgccgcca cattaaaaaa
     8881 cgcgtgccag aggtactcga tttagttggc ttgaaacata agatgcgttc ttttccaagt
     8941 cagctatcag gaggggagca gcaacgtgtg gccattgcgc gtgcgattgt taacaaccct
     9001 aaattattga ttgcagatga accgacaggg aatttagatc ctgagatttc atgggaaatt
     9061 atgcagttac ttgaacgtat taatgttcag ggaacaacta tcttaatggc gacacacaac
     9121 agtcatattg tgaatacatt tcgtcatcgt gttgttgcta ttgaagatgg tcgcatagta
     9181 cgtgatgaag aaaaaggaga ttatggttac gatgattaga tactttttcc gtcatatttg
     9241 ggaatccatt aaaaatctta aacgcaactt ttggatgaca tttgcttcag ttagtatggt
     9301 ggctgttaca ttgactttag ttggtgtttt tgcggcgaca ttgttgaata tacagcgtgt
     9361 tgcttcgggt gttgaaaata atgtccatat caatacctac ttacaagttg attcaactga
     9421 tgctgcaaaa gttattcaaa atactgccgg tgaacctgtt aataacgata actaccacag
     9481 tgtttacgac aaaattgctc agataaaagg tgtcaaaaag attacatttt ctagcaagga
     9541 tgaacaattg aaaaaattgc aagaaacctt aggtgatgtt tggaatatgt atgaccaaga
     9601 cactaatccg ctccaagata tctatcttat tgagacccag acgcctaaac aagttaaggc
     9661 gatcactaaa aagatcagaa cgattgaagg cgtagaagcc gcagattatg gagggattaa
     9721 ctccgataaa ctatttaagt tttcaacatt aattcaaact tgggggttga tcggaacagc
     9781 aatgctatta tttgtagctg tctttctcat ttcaaacact attcgtatga ctattatgtc
     9841 tcgtaaacgc gacattgaaa ttatgcggtt agttggtgct aaaaattctt atattcgagg
     9901 gcctttcttt tttgaagggg cttgggttgg cttgttagga gctgttttac catctcttct
     9961 gatttattat ggatatgatc ttgtttataa gcattttgca caagaattac aacgtaataa
    10021 tctctcgatg tatccgcttg atccttatgt atattacctg attggtgctt tgtttgtcat
    10081 aggtattatg attggttcgc ttggttcagt gctttctatg agacgttatt taaaattcta
    10141 aaaaaagcgc tgctgacgaa acgtagcgac cctcaaaagt tggacctctt atgttattcc
    10201 aaggacttcg ttttgtattg cacaggactg gtccttttca ttttgttttg attcatttgt
    10261 ttatttgtaa taggcaatat agtttaaaat agcagtttct aaggtttcaa gagaagaaaa
    10321 gcaccccaaa agttagatgt ttgatgtatc taacttttgg ggtgtcattc aaaatttagt
    10381 ggctcttttt tagtaaaata ctatgatttc agttttagga taacctcaaa cagtatctaa
    10441 aatagctatg tcaatgaaaa aaggggttag catttttttc gtgacaaatg gtagtagaag
    10501 gaccatgccc aggataaact cggtaatgat taggtaaggt gaatagttct tggcgaatac
    10561 cagtaatgag gtcctcaaag ttactggtag gtaagtcagt acgaccgata gtttcacgaa
    10621 atagagcatc gccagtaacg actaattcat ctgagtggaa aacaaatgag actcctcccc
    10681 aagaatgacc aggtgttggt aaaactgtga attcaaaacc atttagttga tagggttgct
    10741 ttaatttaaa aaagttctct gccggtcttg ctataacagt aatgatatca tcatgacggc
    10801 ctaacccaga taaattatca tctggggaag atagccaagc ggcttctttt tctgaaacat
    10861 aaacaggtgg atgatcaaaa gcatctctga ccaaatctaa actaaaaata tgatcataat
    10921 gagtatgcgt taagaggata gccactaaag gtttttcaaa ggatttaatt ttagcaatga
    10981 tttcttggcc gttacttcct ggatcaatga ggataacagc ttgatcatta acgaggtaat
    11041 aagtattttc accagcaatg tggtttattg ttttatagat tttcataagg atagtgtacc
    11101 aaaaaatcaa ggctttgtta aaaaaagagt ggcggaaaat aaatggcaat tagtttcaga
    11161 aaatatgaga tctcacagca atttcttgac accgttttca taaaggtgta tcataaaagt
    11221 aatcaataag ccaagcctaa agctgaactt agtttaagaa cactgacttt ttgagagcaa
    11281 cctaatcggt tgactaaaaa atatgaggtg gaagatatgt caaaagttgc tattgttact
    11341 ggtgctggtc aaggaattgg atttgctatt gcgaaacgtc ttcatgcaga cggctttaaa
    11401 ataggcattt tagactataa tgaagagact
//
LOCUS       DAUVUY010000060        11297 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.060, whole genome shotgun sequence.
ACCESSION   DAUVUY010000060 DAUVUY010000000
VERSION     DAUVUY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.060"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1251
                     /locus_tag="VOX58_001365"
     CDS             <1..1251
                     /locus_tag="VOX58_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878592.1"
                     /translation="FSSTRNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFIVSLLK
                     GNKKRLYRPLSNIIYPKFNYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIE
                     RLTPSAIEYNVKSENGQVNVTTILENDQVSLVDVDLLRLNTNPEVTITESGQTIPYFD
                     IINYSDYKELMKAQGRINSIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVST
                     HNVFSFSTSVTTLVVSDKLYAKLSSCFPEKEMTIRTFNGNSIRSSEAFYNQFSMVPDV
                     ISSYSKEYTVKTANIATYIFITFLSILFIICTGSILYFTSLIEIMENKEEYGYLSKLG
                     YSKKMIHRIPRYETGILFLIPVFIGIVNGGMLLIYYKYLFMDTLVAGNIIMLSLLLCL
                     LFFLIIYGTFYVLTLRSVTSIIKN"
     gene            1291..2853
                     /locus_tag="VOX58_001366"
     CDS             1291..2853
                     /locus_tag="VOX58_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HES5878593.1"
                     /translation="MIRIKNITKSKFFGTAIILLQQLIALLILVYNRENLSLLFSEKV
                     ALVMTLIDTAFIWLATILRQKQGDIFKRIISIISLTIWQYLVSVLTRGTPLFLSSGLQ
                     IILLYCYTVEITNLILYGHKQFKDKLDKGLLIIIFVSITSLFVNSILFNFLFLMVFTI
                     LHLYPLLVIVLYYRSFRQQISVVRRSLILFSLLLLVILGSELYGEMLDVNQAFNNLGW
                     YLFPLIMSVIYYFKTIHDKLSFVTQRWLGDYKARIELLFLLLILCWIVLMKVLVKDFL
                     LFFIVVDASTLFCLVVISCIFYYLKDSKQNFDYENRRLNYFMKSEENMRVEFSNYLHD
                     DVLQNIIAIKNLLSLENSNITHGFIVNELNDLVSGIRDEMDTYYPIVPANQTMKENIQ
                     SLFDDIVKSRKSNTLLYFNCSDNMVVPSPYGDIVYRFIKELINNAIKYGDGKDIRLSL
                     TIQSDIIIIEESNQVVEKVHSISYGRGLKSFQETLAAFDGDLELQMDTKQFTIRILLP
                     IDWKLCYEDFIN"
     gene            2834..3439
                     /locus_tag="VOX58_001367"
     CDS             2834..3439
                     /locus_tag="VOX58_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5878594.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDITLLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(3991..5571)
                     /locus_tag="VOX58_001368"
     CDS             complement(3991..5571)
                     /locus_tag="VOX58_001368"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HES5878595.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLFAVCDNLEEHFNTFTDAQKIFENAYQDEGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGWEDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDSHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(5584..6771)
                     /locus_tag="VOX58_001369"
     CDS             complement(5584..6771)
                     /locus_tag="VOX58_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HES5878596.1"
                     /translation="MTKSKRPQYRFDSFEGEWEEKKLGEISNIVRGASPRPIQDPKWF
                     DAKSDIGWLRISDVTEQEGRITYLQQRISELGQEKTRVLKDPHLLLSIAATVGKPVIN
                     YVKTGVHDGFLVFLDPKFNREFMFQWLDMFRPYWNKYGQPGSQVNLNSEIIRNQVINL
                     PSLPEQEAIGELFQTVDQLIQLQRQKLAILKEQKQTFLRKMFPAQGQKVPEIRLQGFD
                     GEWEEKELGDIVQITMGQSPSSQNYTTNPSDYILVQGNADIKNGYVFPRVWTTQITKQ
                     ADKGDIILSVRAPVGDVGKTNYHVIIGRGVAAIKGNEFIFQILKYLKEIGYWKRISTG
                     STFDSISSSDIKYAKIQIPSLSEQEAIGNFFQTLDQQIAQSEEKLTELKALKQTLLNR
                     LFV"
     gene            complement(6784..9762)
                     /locus_tag="VOX58_001370"
     CDS             complement(6784..9762)
                     /locus_tag="VOX58_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HES5878597.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTASAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVATTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRYLTNTFRNSRWYGFTGTPIFVENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLDSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLMKKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDTALSLYSNGGQSEVLAPSWEEEKARFFEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAFQAFDKLYASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIAYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGISTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            10043..11185
                     /locus_tag="VOX58_001371"
     CDS             10043..11185
                     /locus_tag="VOX58_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HES5878598.1"
                     /translation="MTINRVLKDDNLLQYTEKEFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVTYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIASNFAKKVFYQKTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILVANLMLLIAQFNK"
BASE COUNT         3462 a         1880 c         2097 g         3858 t
ORIGIN      
        1 ttttcatcca cacgaaatat gctattaagc atagggatgg taccggtttc tctattgata
       61 attgtcttag ttgttttagg aacagtgttc accatcagat atggattggc ttttatagtt
      121 tcgttattaa aaggaaataa aaaaaggtta taccgtcctc tgtctaatat catctatccc
      181 aaatttaact atcgtattgc aacaaaaaat aaattattaa cagtcttggg aggtctttta
      241 acagtaaccg tttcagttgc tggaatgatg gtaatgctct atgcttattc tcttaatggg
      301 atagagaggt tgactccatc tgccatagaa tataatgtta aatcagaaaa cggtcaagtc
      361 aatgttacaa ctattttaga gaacgaccaa gtgagcttgg ttgatgttga cctattgaga
      421 ttgaacacta acccagaagt aactatcaca gaatctgggc aaacaattcc ttattttgat
      481 ataattaatt acagtgatta taaagagtta atgaaagctc aagggagaat aaattctatt
      541 gaaggtagta agtcactccc attgttaata aattattatc caacagaaat tagccttgga
      601 aaaaccttta acttaggaaa tgcatatgat gttactgtaa aacaagtatc aacgcataat
      661 gtttttagtt tttctacaag tgtcacgacc ttggttgttt ctgataaatt atatgctaaa
      721 cttagttctt gttttccaga gaaagaaatg acaattagga cttttaatgg aaattcgatt
      781 aggtcaagtg aagcatttta caatcagttt agtatggttc ctgatgttat cagtagttat
      841 agtaaggaat acacagtaaa gactgctaat attgcgactt atatctttat aactttccta
      901 tccatactct ttattatttg tacaggtagt attctgtact ttacaagcct catcgaaatc
      961 atggaaaata aagaagaata tggctatcta agtaagctag gttatagtaa aaaaatgatt
     1021 catcggattc ctcgatatga aacaggtata cttttcctta ttcctgtatt cattgggatt
     1081 gtaaatggtg gtatgttgct tatttactat aaatatttat tcatggatac attggtagca
     1141 ggcaatatca taatgttatc tttattgctt tgtctgcttt tcttcttgat aatatatggc
     1201 acattttatg tattgacatt gcggtcagtg acatccataa tcaaaaatta attttaaaaa
     1261 tcttttaact ttaacgatta agtaaataat atgataagaa taaaaaacat aacaaagagt
     1321 aagttctttg ggactgcaat aatcttgttg caacaattaa tagccttgtt aattttggtc
     1381 tataatcgag agaacctgtc tcttcttttc tctgaaaaag tagctcttgt aatgaccctt
     1441 attgatacag cttttatttg gctggcaaca atattacgcc aaaaacaagg tgatattttt
     1501 aaaaggatta tttctatcat ctccttaaca atttggcaat acttagtatc tgtgctgaca
     1561 agaggaactc ctctcttttt aagcagtgga ctacagatta tattgctcta ttgttatacc
     1621 gttgagatta ctaatttaat attatatggg cacaaacaat ttaaagataa gttagataag
     1681 ggcttgttaa taattatttt tgtttctata actagccttt ttgttaatag cattttattt
     1741 aattttttat tcctgatggt ttttactatt ttacacctgt atccgttact agttatagtg
     1801 ctatattata ggtcctttag gcaacaaata tcagttgtta gacgttcatt gatactattt
     1861 tctttacttt tgctggtaat acttggtagt gaactttatg gagaaatgct agatgttaat
     1921 caagccttta ataatttggg atggtatctg tttcccctta taatgagtgt aatatactat
     1981 tttaagacca tacatgataa attgagtttt gttacccaaa gatggttagg tgattacaaa
     2041 gctcgtatag aactattatt tctacttctt atcctttgtt ggattgtact catgaaggta
     2101 ttagttaaag attttttgct attttttata gttgttgacg caagcacatt gttttgtcta
     2161 gtggtaattt cttgcatctt ttattattta aaagactcca aacaaaattt tgactatgaa
     2221 aatagaagac tgaactattt tatgaaatca gaggaaaaca tgagagtgga gttctcaaat
     2281 tatttgcatg atgatgtttt acaaaatatc atagcgataa aaaacttgct ttctctagaa
     2341 aatagtaata taacacatgg ttttattgtt aatgagttga atgatttagt ttctggtatc
     2401 agagatgaaa tggatactta ctacccaata gttcccgcta atcaaacaat gaaagaaaac
     2461 atacagtctc tttttgatga tattgttaaa agtagaaaaa gtaataccct attgtatttt
     2521 aactgttcag ataatatggt agtgccatcc ccatatggag atattgttta taggtttata
     2581 aaagagctaa taaacaatgc tataaaatat ggagatggaa aagacattcg tttatcttta
     2641 acgattcaat ctgatattat tatcattgag gagagcaatc aagtagtgga aaaagtacac
     2701 tctataagtt atggcagagg attgaaatca tttcaagaaa cactagcagc ttttgatgga
     2761 gatctagaac tacaaatgga tactaaacaa tttactatca gaatattact tcccatagat
     2821 tggaaattgt gttatgaaga ttttattaat tgatgaccac agactatttg caaagagtat
     2881 ccaactgtta ttccaacagt atgatgaagt agatgtcata gatacgatta cttctcattt
     2941 taacgatgtg acgattgatt tgtctaagta cgatattaca ttgcttgata ttaatttaac
     3001 aaatatttct aaagaaaatg gattggagat agcaaaagaa ctcatacagt ctactcctca
     3061 tttgaaagtt gttatgttga caggatatgt taaatcaatc tatagagaga gggcaaaaaa
     3121 agtaggagca tacggttttg tagataaaaa tatagaccct aaacaactca tttctatctt
     3181 aaaaaaagtg gattctggaa aaaagtattt tgaacaaatc gaatctcagg attatgttga
     3241 atctttaact gaccaggaaa tagctatctt aaatttgagt aagaaaggat tctctataaa
     3301 agaaatagag gaaacgttac aaattagtag gagaacagta tttaatcatt tgacccacat
     3361 ctactcaaag cttttggtaa ataataagca agaggctatc tacaaagccg aacaattagg
     3421 gtattttatg gatttctgag ttgcagataa tcaaacgaca taagttttac ttgtgtcgtt
     3481 gctagggatt tcctgttaaa tacccagatt taaactcatt gtttttgagt tctttttgat
     3541 tgaaattgat tgagataacg cttttataaa aagtagggca atgcctgata tgaaaagctt
     3601 tttaaattta gtcaaacaaa agttgtttaa accaggtcta aaaaaactcg taaagcttca
     3661 caactcccag aacgttaata tatgcttata tatcaacgat tggaactact ttatggttcg
     3721 caccatggtt tttgtggaag gatcaaaagt tgtcctgaaa atttctctta accgtgttta
     3781 agctttctaa acgtttgaga aatcattgta acttcaaata actattttaa ataaaacaaa
     3841 gaaacggaca aagttagatg agatgattct attttgattt ataggtgaag aaggagaatc
     3901 ttgtttgata agtactcaat aaaggttcac tacccaattt taaataaaac gccacagtag
     3961 taatcatcta ctgtggtgtt ttttggtctt ttagtttcta aaagtatcca attcgtcttg
     4021 tgcttctttg gttgttccga ctagttgttt catgagttga tctagtttgg catttgtttt
     4081 ggcaatttct tgatcaatgt cactgagctg tttagctaat tctggcagtg gtttgacggg
     4141 gacttcctca aaggtatcga catagcgtgg gatatttaag ttatagtcat tttcaatgat
     4201 ttcatcaaat gaggctaaat atgaaaactt atcagaatta tcacgactct tataagcatc
     4261 aaggatcttt ttgatatggc tatctgtcat tgtgttttga tttttgcctt tatcaaactc
     4321 ttttgaagca tcgataaaga agacatcttt gttggttctg ttcttcttta atatgataat
     4381 agtcgtaggg atgcttgtat tataaaagat atttgatggg agtccgataa tggtatcgat
     4441 agccccttgt tcgagtagtt tttggcgaat cttgccttct gcagcaccac ggaaaaggac
     4501 accgtgtggt aagacaatcg ccatagttcc tgtatttttt aaatgataga aaccatgaag
     4561 taaaaaggca aagtcagctt ttgattttgg tgctaataca ccataacttg aaaaccgtgg
     4621 atctgttaag aaaccagcag tcgctgacca tttcaaagaa taaggtgggt tcattaacac
     4681 tccatcaaag tttgttggtt catctgttgg ccagtctgca tccaaggtat cagcgttact
     4741 taagtgttgg ttttcgatgg caacgccatg aagcatcatg ttcatacgag ccaagttata
     4801 agtagaagta ttgatttctt gaccatagta agagacggta tcggattggt tactatattt
     4861 cttagcattt aaaaggagtg atccagatcc catagcaggg tcataaaggg tcattccttt
     4921 ttgatcttcc caacctaaaa agacaatctg agtcatcaag tgagagacag cttgtggggt
     4981 atagaactca cctgcttttt ttcctgactc actggcaaat tcaccaatca agtattcata
     5041 agcatcacct aaagtatccc catctactgc ctcaaagtca atttcattta atgttttcat
     5101 gacattagaa atggtttgat tttgtttttg aggagtagag cctaattttt tagagtagag
     5161 gtcaatatct tcaaataagt tttcaaagtc ttcaccactt tgttcaatat cgcgaaaacc
     5221 ttgagctaat gattctaatt ggaaagtatt gtgataaaca tcttggatga gtttttcaaa
     5281 ggtcagtgta ggttcgataa agtaacctaa atcaccagta acaacagaaa tgaggtcatc
     5341 ttttaggcct tcatcttgat aggcattttc aaagattttt tgagcatctg taaaggtatt
     5401 aaagtgttct tctaaattat cacaaacagc aaagagtaat ttatcagata aatgtttgta
     5461 aaagatgagt ccaagaagat agtttttata gtcattggca tccatttggc cacgtaattg
     5521 atcggccgaa tgccatagtg cttgccgtag tgaagttgtt ttctctgcca tgtgcgtcct
     5581 ttcttatacg aataaacgat tgagtaatgt ttgtttgagt gctttgagtt ctgttagttt
     5641 ttcttcagat tgagcaattt gttggtctaa agtttggaag aaattaccaa tggcttcttg
     5701 ttctgagagg gaagggattt gaattttagc atatttgata tcactagagc taatgctatc
     5761 aaaagttgaa cctgtactaa ttcttttcca ataaccaatt tctttcaagt attttaatat
     5821 ttgaaagata aattcattac cttttatagc agcaactcca cgcccaataa taacatgata
     5881 atttgtcttt ccaacatcac caactggagc cctaacacta agaataatat cacctttgtc
     5941 agcttgtttt gtaatttgtg ttgtccagac acgcggaaaa acatatccat tttttatatc
     6001 ggcatttcct tgtactagaa tataatcaga agggtttgta gtataatttt gacttgatgg
     6061 agattggccc attgtaattt gaacaatatc acctagttcc ttctcttccc actcaccatc
     6121 aaaaccttgt aaacggattt cagggacttt ttgtccttga gctgggaaca tctttctgag
     6181 gaaggtttgc ttttgttctt taaggattgc tagcttttgc ctctgtaact gaattaactg
     6241 atcaaccgtt tggaagagtt cgccaatggc ttcttgttca gggagggagg ggagattgat
     6301 aacttgattt cgaattattt cagaattcag attcacttgg cttcctggtt gaccatattt
     6361 attccaatat ggtctgaaca tatctaacca ctgaaacata aattcacggt tgaatttagg
     6421 atctaaaaac acaagaaatc catcgtgtac tccagtttta acataattga taactggttt
     6481 acctactgta gctgcaatac ttaatagtaa atgtggatcc tttaatactc tagtcttttc
     6541 ttgaccaagc tcagatattc tttgttgtaa ataagttata cgaccctctt gttcagtaac
     6601 atcagaaatt cttaaccaac ctatatcact tttggcatca aaccattttg gatcttgtat
     6661 cggtctagga ctggcaccac gaactatatt agaaatttct cccagctttt tctcttccca
     6721 ctccccttca aaactgtcaa agcgatattg aggccgttta gattttgtca tctctttcct
     6781 ttcctagtgc ccttttaagg gttggatttg atctctaatg aattgaacta actcccgttc
     6841 aaattggctt ttgtatttga gtttattcat ggccgagtca ccttgttttt ctttataggc
     6901 ttggtagtct agggattctt ttaaggttga tatgcccact tgtttggtag agttagcact
     6961 atgataatta gtcgccacaa aggctaattt gtcaggatct gccttccact ggtccgcaaa
     7021 gtgtttgatg gccctttgga tcttatcacc aatgaggtta tccaactcat aagcaatgct
     7081 tttcccttca taggctttgg gttctttttg gatatcttgc cataactcag ctagtgagtc
     7141 agccatagca gggtttgatt ttgccaaatc ttggatgtat tgatccatgg gattgtcatt
     7201 gagcctttct tgaagagctt cttgttcttg atcgacaaag gcttgaatta aggtcaagat
     7261 atagtgatag ttgatatcat ccatctgaac actttctagc tcatagtcga tattgatctc
     7321 tgggatggct tcgtcatctt ctcttcgctt tcgtatttct gcaatgacat tttggtaagt
     7381 gcctttataa gtatcaatca cttcatctga taacccaacc tcactcaata gtgtttcatt
     7441 aaagtcacta tagacctgta cagatgcata gagcttatca aacgcttgaa aggctttggc
     7501 gtattgtttg agaaaagctg tctgtgcgct ctcaatagtt gggaaagcgt ctggatcagg
     7561 aactatattt tttaatactg ttactttttc aaagaatctt gctttttctt cttcccaaga
     7621 aggcgctaaa acttcacttt gaccaccatt tgagtatagg ctaagagccg tatcaacagc
     7681 ttctttaaaa cgaagtggtg tttggaaagt cacaacctga ccgtaatgtt ttctactttc
     7741 aaagatacgg ttggttcgtg agaaagcttg aataatgtgc tgaggtttca taggctggcg
     7801 atcgatgaag atggttgata aacaaggcgc atcaaagcct gttaagagac ggtcaaccac
     7861 aatcacgagg tctaattgtt catgacggtc tttgaattta tcttttttac gggctaagcg
     7921 atcatttaaa tctctgttat agccttgtaa gttatcgatg gtaaagttag tgccaaagag
     7981 gtgattatag tcttctagat ttttggtcat tttatcttgt cggctgatag acgcattgtc
     8041 attttcagtg atagaataag taatagctac ctttggaaag tctggtagtt tttctttgac
     8101 cttcttagaa atgactaaat ccgtttcacc agccttaact ttcttcatca gatcataata
     8161 ggcttgggca cgagcaatac tcttaacggt tagtaaacct tcgaatgtct gaccaattcc
     8221 attattaaaa ccaagtttct tgcgtgattg attaatgata ctatctaaga cagctaacat
     8281 atgttcttca tgatcataag cttcttccgg aatactgtct tcaggcatat caggaatggt
     8341 tgtcttatac tcaacttgaa agcctagaac cgctttatca tgaatggctt ctttaacggt
     8401 gtattgatgg agacatttgc cgtattgctg ttccgttgtt tgggctaaat cacctaattg
     8461 agcacgctta ttttccacaa agataggagt gccggtaaag ccataccagc gactgttacg
     8521 aaaagtattc gtgagataac gttgacgttc cggtgtgaca gcacggtggc attcgtccac
     8581 aacaaagaca acatttaagt gtgcaagacg ctcttttagt ttcttgaatt ttggagtgtc
     8641 ataactttcc atctgactga tcatggcatt gagtttttga atggtggtgg ccacaacacg
     8701 acgatcactg gattctaaat ttttgatgag ttgtctcgta tcttctgtct catcgacatc
     8761 aaagatatca ttttgcgcat aggattgaaa agcagaagcg gtctgattat ctaaatcctt
     8821 tcggtcaatg acaaagatgg atttttcaac ggccggtatt tgaaggatat tgcgagccac
     8881 tttataggaa gttaaggttt ttccagaacc tgttgtgtgc cagatatagc cagacttgcg
     8941 gtgacgactc gcttcagcga cggcttcgat tgcatgaatc tgataaggac gaaggagaat
     9001 tagtgctttt ttatcatcat caatgacaga gtaagtcatg accatttggt gagcacgagg
     9061 gatagatagg acttctttgg caaaggcaaa gagatctttt tgtggtttgt tgttttgatc
     9121 tacccattgg gtcaagaagt tagggtttaa tttattttct ttagctgcag cgatatacct
     9181 tgtatctgtt ttatttgaga ccacaaacat ttgtaaggtt gaaaagatac ctctaaactg
     9241 gccttcttta tcatactttt tgacttggtt aaaagcttca atgcattgat gattctgaga
     9301 ttttaattca atttggatca taggtaagcc attgatgaga agtgtcacat cacctcgtct
     9361 atcacgagaa ccagagaaag caacttgatt ggcaatctca taaacagaag ttccaccagc
     9421 cacattgtct gctttaacga cttctaaacg aatcgttcct agttttgcat cttcgcgttg
     9481 cacttgtact ttagcaatgc cgttttcacc agctaaccat ttagccgctt catagtaatt
     9541 gacaaagtta agttgattct taatctgctc tttttcacta gctgttaaag gctcctcatt
     9601 cagatattgc gtgttatttt gcgctaagat cttaaagaaa ttatcccata aagcatcttc
     9661 agtttttaat tctttacgat atgtccattg actttcacct gtttctaaga gatggatcaa
     9721 ctctttttca agttcaagtt ccgttttagt ttgatttccc atttttaatc cccattcgac
     9781 atgatatagt aatattatat cattggcgga tcctttttag aatggcaaag ctattgaaac
     9841 cttccaaaca gtcttttttt gattagttga atgcttaaga tcctagcaac taaacaaagc
     9901 tatgactatg taaatgcaat cgagaaagtt cttgtatttt tgagtttgct tgacaataat
     9961 ttaaggcgaa gaaagtaata agacaacact agtaaaaagg tcgagttctc aaaatagata
    10021 aaaaacttaa gacagatcta gcttgactat taatagggtt ttaaaggacg ataatctgct
    10081 acaatataca gaaaaggaat ttattgaaga tatgaaaatt ttggtagcca tagattcttt
    10141 taagggatcg gtaacgtctc cagaacttaa cactagtgta gctcaagccc ttctttctgt
    10201 cgataagcag ttagttattg aaacaagagc tatcgctgat ggaggagaag gaagtttagc
    10261 agccctctca caaaccgtgg cggggaggtg gcatcaagta aaaaccattg atctattaag
    10321 acgcccgatt aaagtcgctt attatcgtca cgcaaagcaa gcttttattg aatctgcgag
    10381 tattattggt attgataaga tcacccccaa ttcagtaacc tatgcacaag ccacttccta
    10441 tggtttgggt ttggcagtta aagatgccat tcaaaaagga gctactcaaa tcgaaatcat
    10501 gctaggagga acaggcactt ctgatggtgg caaagggttt ttggaaagtt taaactatga
    10561 tttcatgaca ggaagatctt atcttgatac tttagcatca cctgttacgc tattagggct
    10621 gactgatgtg accaatccat atcatggccc tcaaggattt gctgcggtct ttggccctca
    10681 aaaaggtggg agtcttagtc aaattgaaga aactgatcaa attgcaagta actttgctaa
    10741 aaaagttttt taccaaaaaa ccattgattt acagacaata ccaggaagtg gagcagctgg
    10801 aggattagga ggtgccattg tcctattagg aggcacctta actagtggtt ttagcaggat
    10861 tgcagagcta ctaaatctgg ataattccct tcagtcctgt gatttagtta ttactggaga
    10921 aggatgcctg gatacacaat cacaatctgg caaggtacct gttgcgattg caagaatggc
    10981 taaaaaatat caagttccaa cgattgctct atgtggttct gtaaagattg agacaggact
    11041 agcagcagaa gactttttgg ctgttttttc aatacaacag cagccaattt ccttagaagc
    11101 agcgatagat aaaacaacaa cactttccaa tatcaaaata ttagtagcta accttatgct
    11161 attgatagcc cagtttaata agtaaactca tctattttcg attgtgtagc attcatttgg
    11221 cggttaaaag taacctatga agaaggagac gtacgagcta actcattagc atagtatgaa
    11281 aaacagaaac attttgt
//
LOCUS       DAUVUY010000061        11228 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.061, whole genome shotgun sequence.
ACCESSION   DAUVUY010000061 DAUVUY010000000
VERSION     DAUVUY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.061"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..536
                     /gene="sdaB"
                     /locus_tag="VOX58_001372"
     CDS             <1..536
                     /gene="sdaB"
                     /locus_tag="VOX58_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HES5878599.1"
                     /translation="ILYSKLDELGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWT
                     GNPNHVKYKIEWLNGLSYVGDFWNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKG
                     GMRYTEQRAQEWLEANRDGYLYYEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYN
                     TANGYTINYHNGTPTQK"
     gene            complement(777..1619)
                     /locus_tag="VOX58_001373"
     CDS             complement(777..1619)
                     /locus_tag="VOX58_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HES5878600.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            2118..2288
                     /locus_tag="VOX58_001374"
     CDS             2118..2288
                     /locus_tag="VOX58_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878601.1"
                     /translation="MDNPKKDKGLSLGVVDPITILITESVINKMRTVPYHLKKLSSTT
                     YCLLADLAYQSA"
     gene            2560..3756
                     /gene="speB"
                     /locus_tag="VOX58_001375"
     CDS             2560..3756
                     /gene="speB"
                     /locus_tag="VOX58_001375"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HES5878602.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSVGSSRVQRALKENFGYNQSVHQINRSDFSKQDWESQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            3758..4063
                     /locus_tag="VOX58_001376"
     CDS             3758..4063
                     /locus_tag="VOX58_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HES5878603.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMIEAYQTQINSLD"
     gene            4541..5470
                     /gene="prsA"
                     /locus_tag="VOX58_001377"
     CDS             4541..5470
                     /gene="prsA"
                     /locus_tag="VOX58_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HES5878604.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTIAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(5746..5982)
                     /locus_tag="VOX58_001378"
     CDS             complement(5746..5982)
                     /locus_tag="VOX58_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878605.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPAAHGPCD
                     DIHQLLEDKSLSDDYLIATSAMRHLWYWGYSQIG"
     gene            6406..9486
                     /locus_tag="VOX58_001379"
     CDS             6406..9486
                     /locus_tag="VOX58_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HES5878606.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSASSSTESSTTT
                     ANTGTGTASGMTATTPSATTDTGEAAGSGARSEANGASSVVSSEESQSSGTTPASPQA
                     QTAPAATSTSSASSSNEKTPKTATTTTSSTPVASTSNNSNKVTSTEAETQTMDVERYT
                     VDKENSKLNIKDGKTPKTRSSVNKDTKLIRNRDDKQRDIVDVTRTVKTNEDGTIDVTV
                     TVKPKQIDEGADVMALLDVSQKMTKENFDKAKEQIKKMVTTLTGEPTDGKENHNRRNS
                     VRLMTFYRKVNEPIELTAENVDKTLDEVWKKAKEDWDWGVDLQGAIHKAREIFNKEKE
                     KKSGKRQHIVLFSQGESTFSYDIKNKSDITKTKITEKVTTSSPLFPWLPIFNHTNRKA
                     EIIGDLEKVLDMAEKVGISLPSSLKLAVKALGLTNSAIGSILGKGLTEYLGLTEYSSD
                     NLDGGGFDYSKRVGEGYYYHSFSDRKYENTMPLEEAIRTALASNFPKLTDNWFFDILN
                     SFVNKDTVEKAKLDVIMKVLNSIFYKREYRYYNHNLSAIAEAKMAQQEGITFYSVDVT
                     DLNSASKRVRRQAAVRKGTKEEENKKNEEERNTKFDTYLKKMSEGNNFLSNVEERDKF
                     KDTLTELTIKDEFTDKVTVEKDSWSKSITDGLKNSNNNNVKHQQANTSTWSFFSSSKE
                     SLTWIISKEQLKEAFEKNGSLTFKYKLRVNKDKLLDKDKKRTKRDTPTENKTSVTADI
                     ISNTVDYKINNQEVKGNKLDAVKLTYTKETVPVPDVEGEVVPIPEKPLVEPMTPLYPA
                     IPNYPTPQLPKDEDLEISGGHGPIVDIVEDTGTGVEGGAQNGVVSTQENKDPIVDITE
                     DTQPGMSGSNDATVVEEDTAPKRPDVLVGGQSDPIDITEDTQPSVSGSNDATVVEEDT
                     VPKRPDILVGGQSDPIDITEDTQPGMSGSNDATVIEEDTKPKRFFHFDNEPQAPEKPK
                     EQPSLSLPQAPVYKAAHHLPASGDKREASFTIVALTIIGAAGLLSKKRRDTEEN"
     gene            9666..>11228
                     /locus_tag="VOX58_001380"
     CDS             9666..>11228
                     /locus_tag="VOX58_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein"
                     /protein_id="HES5878607.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAISNSLEYQAQYERVKEE
                     LDALKEKGPKLGERSLYFRLVNKFLSWFGQVWDPLAIKETDYLLDLIGQVNYLVSNTE
                     FQAPEYKDAKNKLDLAVAGYDLYLAREKKRGDAPDYQVTQLTLEQLKTIKKKIREALE
                     TFAQEVKTIQQKNPDLFPHAYVLNAKSKDITDKAQPFLVNSYYDVFYKQFLFTNTNDG
                     YNYNKEADNLTSYQNLIKELLGLKAEIDQLTKGKTGDDRLEMLMWQSKFTLSDSRKKS
                     HLSAYYTQGEYVDKVVLSEEAPNLRTAKEKVKTLKGILHDYYVETNKPEKAKKYHVED
                     ETLPSQPEAPATPEAPAPVPAPVPVPGQKPAEDERSPQATEPANPSKEDSSTDASQGS
                     HDSENPATDSPSQPQAPDQGNHQSQVPNAKPQTDKTDTPNAPAIPQDTPKAPEAGGQS
                     GPAGNTEEKAPDSGPKESDQSSSKESPSVGEDTTPNQPDVLVGGQSEPIDITEDTITD
                     APPTVSGHNASTQ"
BASE COUNT         3947 a         2322 c         2156 g         2803 t
ORIGIN      
        1 atattctcta tagcaaatta gatgagttag gaaggacgcg tactgctaga ggtacattga
       61 cttatgccaa tgttgaaggt agctacggtg ttagacaatc tttcggtaaa aatcaaaacc
      121 ccgcaggatg gactggaaac cctaatcatg tcaaatataa aattgaatgg ttaaatggtc
      181 tatcttatgt cggagatttc tggaatagaa gtcatctcat tgcagatagt ctcggtggag
      241 atgcactcag agtcaatgcc gttacaggaa cacgtaccca aaatgtagga ggtcgtgacc
      301 aaaaaggcgg catgcgctat accgaacaaa gagctcaaga atggttagaa gcaaatcgtg
      361 atggctatct ttattatgaa gccgctccaa tctacaacgc agacgagttg attccaagag
      421 ctgtcgtggt atcaatgcaa tcttctgata ataccatcaa cgagaaagta ttagtttaca
      481 acacagctaa tggctacacc attaactacc ataacggtac acctactcaa aaataatacc
      541 aaaaggctag acctctgctc actaggccta gctttttaca tcaaaaaaag caatgactat
      601 agaaagtaaa aatactagaa aaagcaatga ttgccgtcat tgctttttat gatttgtcaa
      661 aaagtaaaaa gcaaattgaa aaaatcgccc tggagctgtt gagataaact acaccattta
      721 ttaagatacc aaatcaaaaa gctaacacca taagagcgtt agctttttaa agagcctcag
      781 gacagtttat gtttaatggc ttctaggtag gtttgaaaca tatgatggat cgttttgcaa
      841 ttgagtaatt ggaagatctc taagatctgc acacactttt gctcagcccc ctctttacca
      901 tttatgaggc caataatgcc atcaaaaaaa gacacacaac accgttcata taagcaatca
      961 tcatttagct gataatcttc aatttttgcc aaaatctcag aagctttatc atactcttgt
     1021 cgctgaataa acaaaatcaa catattgaca aacatccgaa tcgattcatt cccataatac
     1081 cttagggtat tgtatttatc gaggttcaaa ataacttttg aaaacaccat ctcaataaaa
     1141 caagactcaa aaataaacat acaattatta aaaagcacag tctcatagtg actccaagtt
     1201 tcaatattaa taagatagtt tgacaaataa gtccgctctg tcagacagtc ttcctcagta
     1261 agagttgcta ataacacctt gaccaaggca aaaaggttct tttcctttgt tgacttacta
     1321 tctttataag aatcaaggat atgatttaaa ccttctatgt ttcggcattc aaaataatgt
     1381 tttgccgtat ccatatcaat aaattcttta tattgtttaa agttattact aataaagaga
     1441 aactcatcaa cattgacatt caagttgtct aagatataaa gtaacttttt cgaagaaatg
     1501 tcaagattat ttttaataaa acgataatag gtttgcctag tgagataatc accacaaact
     1561 tgtttaatcg aaatgttttt tgaatgccta atgaattcaa cggtttcacc aatttccata
     1621 tgtcaagcct tcctagttga tgtcaaaaat acgttacgca tgtacctaat acgtaacaag
     1681 ttgaatgttt cggatgatag tcgcttatga taggtgcata aggtcaatag ccagatgcga
     1741 tagttttatc aactgtcata tgttaaacct ttctaatcta atagatgtta aaaatacgtt
     1801 acgtgtgtac ctaatacgta acaaaataat gggttagcaa aataagcagc tatgatatag
     1861 ccataaggtt aaaaggaggc gcctactatg tggttattgt tactattttt gtagttcctt
     1921 ttgcaaaatg gtagtagata aaaatgagaa gtcaataaaa gcattgacaa acaaaagaat
     1981 cattttgttt atattatcag tatttttaag tttcttgtca actgaaatga gcatctacta
     2041 gccacaatag taactaaaag gctaattgtc agtaaaaatg agataaatca gccaggaaaa
     2101 agcctacgag tcaactaatg gacaacccca aaaaagacaa aggactttca cttggtgttg
     2161 tggaccctat cacgatcctt atcacagaat cagttattaa caaaatgaga acggtcccat
     2221 atcatctaaa aaaactgtca agcacaacct attgtttgct agctgatctt gcctatcagt
     2281 ctgcctagta gatacggcag aagataagag atcaaccaaa agatgataag tgcaacgact
     2341 catagcgtct tttgaccaaa accaattaga ctcacatgag atgagaataa ttgggttggg
     2401 ttgtcagtgt caactaaccg tgttattgtc tattaccatt catggtatca gcgacatcgt
     2461 atgataacca tgcgattcag ctaagtaagg aggtgtgtcc aatgtactgt taaaagcaaa
     2521 tgcagtagat taacttattt tgaaagaggt ataaaaaaaa tgaataaaaa gaaattaggt
     2581 atcagattat taagtctttt agcattaggt ggatttgttc ttgctaaccc agtatttgcc
     2641 gatcaaaact ttgctcgtaa cgaaaaagaa gcaaaagata gcgctatcac atttatccaa
     2701 aaatcagcag ctatcaaagc aggtgcacga agcgcagaag atattaagct tgacaaagtt
     2761 aacttaggtg gagaactttc tggctctaat atgtatgttt acaatatttc tactggagga
     2821 tttgttatcg tttcaggaga taaacgttct ccagaaattc taggatactc taccagcgga
     2881 tcatttgacg ctaacggtaa agaaaacatt gcttccttca tggaaagtta tgtcgaacaa
     2941 atcaaagaaa ataaaaaatt agacactact tatgctggta ccgctgagat taaacaacca
     3001 gttgttaaat ctctccttga ttcaaaaggc attcattaca atcaaggtaa cccttacaac
     3061 ctattgacac ctgttattga aaaagtaaaa ccaggtgaac aatcttttgt aggtcaacat
     3121 gcagctacag gatgtgttgc tactgcaact gctcaaatta tgaaatatca taattaccct
     3181 aacaaagggt tgaaagacta cacttacaca ctaagctcaa ataacccata tttcaaccat
     3241 cctaagaact tgtttgcagc tatctctact agacaataca actggaacaa catcctacct
     3301 acttatagcg gaagagaatc taacgttcaa aaaatggcga tttcagaatt gatggctgat
     3361 gttggtattt cagtagacat ggattatggt ccatctagtg gttctgtagg tagctctcgt
     3421 gttcaaagag ccttgaaaga aaactttggc tacaaccaat ctgttcacca aatcaaccgt
     3481 agtgacttta gcaaacaaga ttgggaatca caaattgaca aagaattatc tcaaaaccaa
     3541 ccagtatact accaaggtgt cggtaaagta ggcggacatg cctttgttat cgatggtgct
     3601 gacggacgta acttctacca tgttaactgg ggttggggtg gagtctctga cggcttcttc
     3661 cgtcttgacg cactaaaccc ttcagctctt ggtactggtg gcggcgcagg cggcttcaac
     3721 ggttaccaaa gtgctgttgt aggcatcaaa ccttagtatg gaaatgcatt tcgttagaac
     3781 agaacctgag gcacgccgca tagctgaaac cttttgtgcc gaaaacacac aaacaaaaac
     3841 ccctatgcgc gtgcagcagt taagctatcc atcagacaca gatcactcag gtggtgagct
     3901 atacatctat gctttgtctc ctgctggatt tatcatcgta tcaggagaca ccagagcgca
     3961 caccatttta ggctattctt ttgataataa cctagacctc aaccatgata atgtcaggag
     4021 tatgatagaa gcttaccaaa cacaaatcaa ctctttggat tgatcggtca aagtagcttt
     4081 tgcccccctc accactcgtt taagacgacc gtgtttctaa aataccacta ccttttaagt
     4141 ttcacgccgg cctaagcgag tgtttgtctc atggagcaga ctgtttccat cgaaaagtca
     4201 cccattgata aaggaggtca tcaacgccta ctaatgatga cttccctaag atgccaaggt
     4261 aaaggactga gttctgtaga gatgggactc agtcctttta cgatttttgg gagttattct
     4321 agccataccg tggcaagaaa acaacactta aaaatagcca acgtcagttt ctgttcatac
     4381 atgactacag ccaaacacca caacctaacc tatcctaaac acagtggctt tctaacgaac
     4441 agtcacaacg agcagcaaca ccaaaaagca agattaagct acgtttaaga ctaatgatat
     4501 aagataacag taattattaa tgagaaaaga ggctcaggac atgaaacaaa tgaataaact
     4561 cattacagga gtggtaacgc tggcgacagt cgtgacctta tcagcttgtc aatcatcaca
     4621 caacaacacc aaactcgtct cgatgaaagg agacaccatc actgtcagtg acttctacaa
     4681 tgagaccaaa aacacagaac tcgcacaaaa agccatgtta agcttggtga ttagccgtgt
     4741 atttgagaca caatatgcca acaaagtctc tgacaaagag gttgaaaaag cctataaaca
     4801 aaccgcagac caatacggta catcctttaa gacagtccta gcacaatcag gcttaacgcc
     4861 agaaacctat aaaaaacaaa ttcgcctcac aaaattagtc gaatatgccg tcaaagaaca
     4921 agccaaaaac gaaaccatct caaaaaaaga ctaccgtcag gcctatgacg cttatacccc
     4981 aaccatgacc gcagaaatca tgcagtttga aaaagaagag gatgccaaag cagcccttga
     5041 agccgtcaaa gctgaagggg cagactttgc agctattgcc aaagaaaaaa ccattgcagc
     5101 cgataaaaaa acaacctaca cgtttgactc aggtgaaaca accctaccag cagaagtagt
     5161 tagagctgca tcaggcctca aagaagggaa cagatcagaa atcatcacag cgcttgatcc
     5221 agccacctca aaacgcacct accatatcat caaagtcacc aaaaaagcaa ctaaaaaagc
     5281 agactggaaa gcgtaccaaa aacgcttgaa agacatcatc gtgactggca aattaaaaga
     5341 ccctgacttc caaaacaaag tcatcgctaa agctcttgat aaagcaaatg tcaaaatcaa
     5401 agacaaagca tttgccaata tcttagccca gtttgcaaaa cctaaccaaa aacaacctgc
     5461 ccaaaaatag agggttgcca gtttatttcc ctaaggttgc ttaaaaagac agccctaacc
     5521 atcactcccc ttttcaaaac accgataatg gaaacgtgtc ctatactgat ggtctagggc
     5581 aatgtcataa agctaaagac tctatccaaa taaggataga gtctttaata tttaaaaacc
     5641 agtcagcaaa aaaaattcac gctaagccta gctagaatga ggcttagcgt gaatggtatc
     5701 agtgatagtc cgttaacata gctgattggg agtcagaaac tctaactacc caatctgact
     5761 atacccccag taccagaggt gtctcatcgc agaagtagca atcagataat cgtctgataa
     5821 gctcttgtcc tcaagcaact gatgaatatc gtcgcacggg ccatgagctg ccggtggctg
     5881 atagctagta ggctcatcag ccaaaacttt tttggagtaa cgctttggtc tcagacggcg
     5941 ccagagggtt gagagagggg tgagaaaaaa acgatgggtc atcttggcct ccttgtctaa
     6001 tgatagtcac cttaacagac aaaagtgtca ataatgtcat tttaaggaga aaaagattaa
     6061 aaatcaaggt cacaaacttt cctttgttaa aaaaacgcga ccctaacaaa gaatctaaat
     6121 acctctcgca cttgagcttc tccttgcgca gctatcttgt gcactcagtt gcctccaaga
     6181 taatgataac gataccatca ttatcctata ggctatcgcg acaacccctt ttttcgcctc
     6241 aacgagtctg acacagctgt catgacagaa aaaagacctc tgcctatcta agagaccatt
     6301 aatttttctc tcactaaaaa actgatttta gaaacgaaga agcatggtgt ataataaggt
     6361 tcagaacaat tatgacataa taatgaaagt aaggttaacg aaacattgac aaattgtaag
     6421 tataaactta gaaagttatc tgtagggctc gtctccgtcg gaacgatgct gatagccccg
     6481 acagttttag gacaggaggt tagtgctagt agtagtacgg agagcagtac cactacagct
     6541 aatactggta ccggtacggc aagtgggatg actgccacta ctcctagtgc tacgacagat
     6601 actggtgaag cagctgggag cggagctagg agtgaagcta atggtgcatc gtccgtagta
     6661 tctagcgaag aaagtcagag ttcaggcact actccagcct caccccaagc acagacagct
     6721 ccagcagcaa cgtcaacatc atcggcttct tctagtaatg agaaaactcc caagacagca
     6781 actacaacta catcatcgac tccagtagca agtaccagta ataatagcaa caaagtaact
     6841 agtactgaag ctgaaacaca gacgatggac gtggaacggt atacagttga taaggaaaat
     6901 tcaaagctaa atattaaaga cggtaagact ccaaaaacta ggagtagtgt taataaagac
     6961 acaaaactta ttagaaaccg cgatgacaaa cagcgtgata tcgttgatgt tactcggaca
     7021 gttaaaacta acgaagatgg cactattgat gttaccgtaa cggttaaacc gaagcaaatt
     7081 gacgaaggcg ccgatgttat ggcccttcta gatgtctctc aaaagatgac aaaagagaat
     7141 tttgataagg ctaaagaaca aataaaaaaa atggttacga ctttaacagg cgagccaact
     7201 gatggtaagg aaaatcataa taggcgtaat tctgtacgtc taatgacttt ttaccgtaag
     7261 gttaatgagc ctatcgaatt gactgctgaa aatgtagaca aaacattaga tgaagtttgg
     7321 aaaaaagcta aagaagattg ggactggggc gttgacttac agggagctat ccataaagct
     7381 cgagagattt ttaataagga aaaagaaaaa aaatcgggta aacgccagca tatcgtcttg
     7441 ttctctcaag gcgaatcaac ctttagttat gatattaaaa ataaaagtga tattacaaaa
     7501 acaaaaataa ctgaaaaagt tacgacctct agcccactgt ttccttggct ccctatcttt
     7561 aatcacacta accgaaaggc agagattatt ggtgatttag aaaaagtact agacatggcc
     7621 gaaaaagtgg gaataagttt acctagtagt ctgaagttag cagtgaaagc ccttggctta
     7681 actaatagtg caataggttc tattttaggg aaaggtttga cagagtacct tggtttgaca
     7741 gaatatagtt cagataactt agatggagga gggtttgatt atagtaaacg tgtaggggaa
     7801 ggttactact accacagttt ttcagatagg aaatatgaaa atacaatgcc ccttgaagaa
     7861 gctatcagga cggccttagc atctaatttt cccaaactca cagataattg gtttttcgat
     7921 atcttaaata gttttgtcaa taaagataca gttgagaaag ctaaattaga cgtaattatg
     7981 aaggtactta atagtatttt ttacaaacgt gaatatcgct attacaacca taacctgtca
     8041 gcaatagccg aagctaaaat ggctcaacaa gagggcatta ccttctattc cgttgatgtt
     8101 actgatttaa actcagcttc taaaagagtg aggcgacaag cagcagtgcg taagggcact
     8161 aaggaagaag aaaacaaaaa aaatgaagaa gagcgtaata caaagtttga cacctatctg
     8221 aaaaagatgt ctgaaggtaa taatttctta agtaatgttg aagagagaga taagttcaaa
     8281 gatactttaa cagagctgac aattaaagac gaatttacgg ataaagttac ggtcgagaag
     8341 gattcctgga gtaaatctat aactgatgga cttaaaaatt caaataacaa taatgttaaa
     8401 catcaacagg caaacacatc gacatggagt ttcttcagtt catccaaaga aagcctcacc
     8461 tggattattt ccaaagagca gctcaaagaa gcctttgaga aaaatggttc tctcactttt
     8521 aaatacaaat tacgggtcaa taaagacaaa ctattagata aagataagaa gagaacaaaa
     8581 cgtgatacac ctacggaaaa taagacttct gtaacggcag atatcatttc aaatactgtt
     8641 gactacaaaa ttaataatca agaagttaag ggtaacaaac ttgatgctgt caagttgact
     8701 tatactaaag agaccgttcc tgttccagat gtggaaggag aagttgtacc aataccagaa
     8761 aaaccactgg tagaaccaat gacgcctcta taccctgcaa ttcctaatta ccctacccct
     8821 caacttccaa aagatgaaga tctggagatt agtggaggtc atggaccgat tgtcgatatc
     8881 gtcgaagata ctggtacagg tgttgagggc ggcgctcaaa atggtgtggt ttcaactcag
     8941 gaaaataaag atccaatcgt tgacatcacc gaagataccc aaccaggtat gtcaggctca
     9001 aatgacgcga cagttgtcga ggaagacaca gcacctaaac gtccagatgt ccttgttggt
     9061 ggtcaaagtg atccaatcga tatcaccgaa gatacccaac caagtgtgtc aggctcaaat
     9121 gacgcgacag ttgtcgagga agacacagta cctaaacgtc cagatatcct tgttggcggt
     9181 caaagtgatc caatcgatat caccgaagat acccaaccag gcatgtcagg ttctaatgac
     9241 gctactgtta tcgaagaaga tacgaaacca aaacgcttct tccactttga taacgagcca
     9301 caagcaccag aaaaacctaa agagcaacca tctctcagct taccacaagc tccagtctat
     9361 aaggcagctc atcacttgcc tgcatctgga gacaaacgtg aagcatcctt tacaattgtt
     9421 gctctaacaa ttattggagc tgcaggtttg ctcagcaaaa aacgtcgcga caccgaagaa
     9481 aactaaccct cgttagtcta gcgaccctaa gcctttggct ttaagaattt ctcattatcc
     9541 ccctcaaaca ccggtacctc actgagatgg cgctttaaaa aggcgctaat ctcaaatcag
     9601 gtatccccta cggttgagag ggattaatgg gtatcacaac aagaaaaaag gtataatgat
     9661 gaaatatgac taaaaaattt attaaatcag cagtgattct aggcttagca agcataacca
     9721 tcagcatgac gcaacccgtt gaagccattt caaatagctt agaatatcaa gcacaatacg
     9781 aacgtgtcaa agaagaacta gacgcattaa aagaaaaggg cccaaaatta ggagaacgtt
     9841 ccctttattt tagattggtg aataaatttc taagttggtt tggccaagtc tgggacccac
     9901 tcgcaatcaa agaaaccgat tacctgttgg acctcatcgg acaagtcaat tacttggtga
     9961 gtaacaccga gtttcaagcc cctgagtaca aagatgctaa aaacaaacta gacttagctg
    10021 tagcaggcta tgacctctac ctagctagag agaaaaaacg tggtgatgct cctgattacc
    10081 aagtgaccca gctaacgcta gaacagctca aaaccatcaa aaagaagata cgagaggccc
    10141 tcgaaacatt tgcacaagag gtcaaaacaa tccagcaaaa aaacccagac ctatttcccc
    10201 atgcttatgt tttaaacgct aagagcaaag atatcacaga taaagcgcaa ccctttttag
    10261 taaatagtta ctacgatgtc ttttataagc agtttttatt cactaataca aatgacgggt
    10321 acaactataa taaagaagca gacaatctga cctcgtatca aaatctgatt aaagagctat
    10381 taggcttaaa agctgagatc gatcagttaa caaaaggcaa gactggcgat gaccgtttag
    10441 agatgttaat gtggcaatca aagttcacct tatctgattc acggaaaaaa tcacacctta
    10501 gcgcctacta tacacaaggc gagtatgtcg ataaggtggt cctatctgaa gaagctccca
    10561 acttgagaac agcaaaagaa aaggtcaaga ccttaaaagg gatcttgcat gattactatg
    10621 ttgaaactaa taagccagaa aaagctaaaa agtatcatgt cgaggacgag accttgccgt
    10681 ctcaacctga ggctcctgct acacctgaag ctcctgctcc tgttcctgct cctgttcctg
    10741 ttcctggtca aaaaccagca gaagatgaga gatcgccaca agctacggag ccagctaacc
    10801 cgtcaaaaga ggattcctca acggacgcat ctcagggatc tcacgatagt gaaaacccag
    10861 cgacagacag tccttctcaa cctcaggcac cagaccaagg caatcaccaa agtcaagtgc
    10921 ctaacgccaa gcctcaaacg gacaagacgg acactcctaa cgccccagcc atcccacagg
    10981 acaccccaaa agcgcctgag gctggtggtc agagtggccc cgctgggaac actgaggaga
    11041 aagctcctga ctctggacct aaagagtctg atcaaagctc atctaaagag tccccatccg
    11101 ttggcgaaga cacgacccct aatcagccag atgtccttgt aggtggtcaa agtgagccaa
    11161 ttgatatcac cgaagacaca atcactgacg ctcctccgac agtatctggg cacaacgcat
    11221 caacacag
//
LOCUS       DAUVUY010000062        11089 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.062, whole genome shotgun sequence.
ACCESSION   DAUVUY010000062 DAUVUY010000000
VERSION     DAUVUY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.062"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            11..640
                     /locus_tag="VOX58_001381"
     CDS             11..640
                     /locus_tag="VOX58_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HES5878608.1"
                     /translation="MTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRPVHFAA
                     SYLFMEIGFRWIVLMSVGFPFLMVLSGIKVMAGLSTLQVLASSCLYLVSLLLAFLINF
                     YFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKVVYIVLSFLPFSSLVY
                     TPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            642..1427
                     /locus_tag="VOX58_001382"
     CDS             642..1427
                     /locus_tag="VOX58_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878609.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFVIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            1513..1662
                     /locus_tag="VOX58_001383"
     CDS             1513..1662
                     /locus_tag="VOX58_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HES5878610.1"
                     /translation="MQLLDNVYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            2057..3205
                     /locus_tag="VOX58_001384"
     CDS             2057..3205
                     /locus_tag="VOX58_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HES5878611.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            3162..4409
                     /locus_tag="VOX58_001385"
     CDS             3162..4409
                     /locus_tag="VOX58_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HES5878612.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDSELLKKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            4465..5499
                     /locus_tag="VOX58_001386"
     CDS             4465..5499
                     /locus_tag="VOX58_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HES5878613.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            5661..6371
                     /locus_tag="VOX58_001387"
     CDS             5661..6371
                     /locus_tag="VOX58_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5878614.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            6364..7716
                     /gene="vicK"
                     /locus_tag="VOX58_001388"
     CDS             6364..7716
                     /gene="vicK"
                     /locus_tag="VOX58_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HES5878615.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWIEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            7720..8529
                     /locus_tag="VOX58_001389"
     CDS             7720..8529
                     /locus_tag="VOX58_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HES5878616.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            8962..9654
                     /locus_tag="VOX58_001390"
     CDS             8962..9654
                     /locus_tag="VOX58_001390"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HES5878617.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVRD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            9655..>11089
                     /locus_tag="VOX58_001391"
     CDS             9655..>11089
                     /locus_tag="VOX58_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HES5878618.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFYKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLKQERLFD"
BASE COUNT         3429 a         1880 c         2321 g         3459 t
ORIGIN      
        1 tctgtctgac atgacctttt acattatcat gagttttgtg accactttac tgaccaaatc
       61 agacagctcc tttatgattg gcgaagaagt gaaagatggt tctatcatca tgcgcctgct
      121 tcgaccagtt cattttgcag cctcttacct ctttatggaa attggttttc gctggattgt
      181 gctaatgagt gtcggctttc cttttttaat ggtcttatct ggtattaaag tcatggcggg
      241 gctgtctacc ttgcaagtcc tagccagcag ttgtctgtat ttagttagcc ttttattagc
      301 ttttttaatc aatttttatt tcaacatttg ttttggcttt tcagcatttg tctttaaaaa
      361 cctttggggg tctaatttat tgaaaaatgc cttggttgcc tttatgtcag gcagtttgat
      421 tccattggct ttttttccta aagtggttta tatagtgctt agttttctac cgttttcctc
      481 tttggtttac acgccagtta tgattgtcat tgggaaatac agccttagtc agataatggt
      541 ggccctaagt ttgcaaatct tttggttact ggtgatggta gtcttatcgc aagtcatctg
      601 gaaaaaggtt cagtaccatt taacgattca aggagggtga gatggcaaaa ttgagacgta
      661 tgcacgctat ttttatcaaa caatacctca aacaaattat ggaatacaag gttgattttg
      721 tggtgggagt gctaggtgtt tttctcactc agggacttaa tctgctcttt ttatcggtac
      781 ttttccaaca tatcccttct ttagaaggct ggacgtttga acaaattgcc tttatttatg
      841 gcttttcttt gattccaaag ggaattgatc acctcttttt tgacaatctt tgggctttag
      901 ggcaacggct tgtccgcaaa ggagaatttg acaagtacct caccagacca atcagtcctt
      961 tgtttcatgt tttggttgag acatttcaag tggatgccct gggtgaatta ttggttgggg
     1021 ttatcttatt agtgaccacg gctggtagta tcgtatggac tcttcctaag gtgcttttgt
     1081 ttattttggt tattcctttt gcgaccttga tttacacctc tttaaaaatt gcgaccgcca
     1141 gcatttcttt ttggaccaag cagtctgggg cagttattta tattttttac atgtttaatg
     1201 acttttccaa gtaccctatg tccatttacc attccttttt gcggtggtta atcagttttg
     1261 tcattccctt tgcttttaca gcttattacc ctgcttctta ttttttaaca ggtcagcacc
     1321 tgctctttaa tattggaggc ttggtagtgg tgtcgttact ggttttggcc ttgtcgctta
     1381 agctttggaa atggggccta gatgcttatg aaagtgcagg ttcctagagg cattgtttgg
     1441 aaccatcatc agcagattgg tctaaattaa gggcggtaaa ataacataag cagatatatt
     1501 tcaagtggtt gcatgcaatt gctagataac gtttatggaa ctttgagatc ttttgccttt
     1561 atgagacctg tcaaatctag taacgatacc agcttagcag atgccttgca ggctgataca
     1621 gacatggctt ttattttagt tagaggcatt aactatcaat aaatagtaaa gacaaggttt
     1681 gtcaatacca taaaaaagtt aagtgtcaca gcttagcttt tttatggtgt ctaattcttt
     1741 attctatata agattttctt ttcttaggaa tggttttcca gtataataaa gtcaccctgt
     1801 atcaaaaaca aaggttacag tttgaatgtt aaagcaacaa agtaagtgac ttctacgaca
     1861 atagttgtgg gacttgacat ttcaatgacg ttagagaatt ttatagattt tattgatggc
     1921 acactagcga gactaaagag attctctaaa ttaatttagg tgtattttta gcagttatta
     1981 gttcaaaaaa gaaaaccatc agaaataatt atataataac catttaagga catcgaaaat
     2041 aaaggaggtc aatataatga cggatgttta tattgctgca ggccttagaa cccctatcgg
     2101 tttagtaggg aaacaatttg ctaaagaaca accagaaatc cttggagcaa agctcatcaa
     2161 tgctttacaa aataagtatc cagttcccat tgaccaagtg atttgtggca atgccgtcgg
     2221 aacgggtggt aatattgggc gcctgatgac cctgtattct catttaggag aatctgtctc
     2281 tgctttgacg gttgatatgc agtgtgcatc agctggtgca gctctttccg tgggttatgc
     2341 taaaatcaag gcaggtatgg caagcaacct tttagtggga gggattgaaa gcagctcctt
     2401 acaacctgaa tcggtatatg cttcagctga ttggcgccaa ggagcctaca aggtagctca
     2461 gttttctcca gatagtatca gcccttttgc gatgattgaa ggggcagaac gagtggcaag
     2521 agagcatggc tttacaaagg agtatttgaa tcactggaca ttgagaagtc atcaaaaggc
     2581 tagttattgc caggagcaag ccctgttagc tgatcttatt ctagacctgc caggagccag
     2641 tgatcaaggt attcgaccgc gcttgtcaag taaggtgttg tcaaaggtac cccctatctt
     2701 aggagaagat cacgtgatta gtgcagccaa tgcctgccta acccatgatg ctgctgcctt
     2761 tttacagcta agtagtcagc cttcagcttt taaactgatt gatgtggtag aagtagcagg
     2821 agacccacag cgtagccctt taatggtgat taaggctagt caagtccttt tggaaaaaca
     2881 tggtctaggg atggcagata tgacagcgat tgaatggaac gaagcctttg ccgttattga
     2941 tggtctattt gaaacccatt atccagattt attggaccgc tataatatat ttggtggtgc
     3001 attagcttat ggtcatccct atggtgcctc agcagccatt ataatattgc acttaatgag
     3061 ggctttagag attaaaaatg gacgctatgg aatagctgct atagcagcag ctggtggcca
     3121 agggtttgct gttttactaa aatatcataa ggagtttagt catgctgaca aagctagaat
     3181 attgggcaaa gcaatgccca aataaaaaag ctatcgtagc cgatcagata agcttgactt
     3241 atcaagagct atggcaagcg gttttaataa aggatcagac aataaaagac agtgtaccat
     3301 atatcatcag tcatagtcgc tatcttaatc agttactctc ttttttacga ggattaaaag
     3361 aaggcagttg tcctatcatt ttacacccta atatctcagg tacatttcag cagcaaataa
     3421 aacacgtcga tagtgagctt cttaaaaaag ctgattttgc tgttttgagt tcgggaacaa
     3481 ctggcaaagc aaaattattt tggcgttgtc tatcaacttg gacgagactt tttgattatc
     3541 aaaataaggt gtttggtatg actggtaata gttgtctatt cttacatggt agttttagtt
     3601 ttactggcaa tcttaactta gcattagctc aactatgggc cgggggatgt ctggttttaa
     3661 gccaaaagct gtctttgaaa acgtggttaa gcttatggca agcaaaaaag gtgagtcacc
     3721 tttacctttt accgacctac ctaaatcgct tgctacctta tttgactaag aacaacatga
     3781 ctgcgactca cttattgacc tcttcgcaaa tgatatcaca agaattacta agacattatt
     3841 acaaaaaatt tcctcagctt gaaattgtca ttttctacgg ggctagtgaa ttatctttta
     3901 taacgtggtg taatggcaga gctgctgtca aaattaatgg tttggtagga cagccctttc
     3961 ctgatgtgtc tatcagtttc aaagataagg aaatttttgt agagactcct tatagtgtag
     4021 aaggaatgtc acaaccatac agtgtctcag acttaggaaa aatgagccca gcgggtttaa
     4081 ttttagaagg gcgacaagat gactgggtta atcaacgagg agtgaaatgc cacctgccta
     4141 gcctagttga actcgctcac caagcgccaa acgtcaaaga agcgcacgct ttaaagatag
     4201 gaaaaggaga aaacgaaacc ctaatattag tattagtttt gactaagaag gattgcctag
     4261 cacctattaa agacttttta gctctttatt taaactctgg acaacttccc aagtactatc
     4321 ttgtgattga ttgtttgcct ttaaaagata atggtaaaat taatcgagaa gttcttttaa
     4381 ataaaatacc caaacagtgg cttagttaac cacttcaggt ttcgttttgc ttatagtgag
     4441 atacaagtga cgattcagct ttagatgtgt tataatatgg tcaaaaaaga tgaggagaag
     4501 gccatgaaac aagctatctt agatcgctat caggccttga aatgctatca aaacgcaggt
     4561 ttatcaaacc aagcatttcg tgctatagct aaagagccta tcattgataa tcgacttggt
     4621 tctccaactt tctgggtaat atggcctatt gaaaaagaaa accaatcagc taagcagtta
     4681 ctaacgtttc ttcttgattt ggtagagatg ccatttgagc taagtggtca gttgcatgaa
     4741 actcaaacgc tactcacccg attccatcca tctctattgc cagatcatat gttttggaaa
     4801 gagttggcta gcttagttga tcaagctttt cctggcaaaa cactaagcca agcaggtgag
     4861 cttgaaaagc gtttgcatca atttcgttat gtcatctcta gtcaacaagc tcaatctatc
     4921 agaaaccatt ataaaatgat tgagatgaca gacgcccaag ctctggccct ttttttgagg
     4981 tctaaaaaag ggccttgttt gtggcggcaa gcaccagatt ataccttgat ggattctgcc
     5041 aggttacata ataaactaag atttgaagat aataaagtca tttttccaag tcaagaagtt
     5101 tcctataata taaaagtact tctttggttt cataccgaat ttattctaga tagcacaggc
     5161 ttttttctta atgaagttga tgcagaagta gtgaccgaaa aaggaattgt caatggtgca
     5221 tcttttaact atggcacaga tggtcctaga cattgggatt tagatgttga tccgattagc
     5281 cgtcacgatc ctcagtttag aagggacacc cttaaaggat ttcgatcgcc aaaaagagta
     5341 tttcgtcagt ggtttagagc tcaaaaagat gactttatgt tctcttattt taatgccaaa
     5401 ggactttttg cttaccacaa caagagtagt tttgccagag tcaaaaaatc tgctaagcaa
     5461 ttcaaacgtc aaattcatcc tataaagggt tggttttaga aagtactcta tttgtaacgg
     5521 tagtcgtcat tgtcaattag gctgaatttt tggtataatc aggtagaaat aatttagaaa
     5581 aatatagttt tgtgattttt tattaaccac aaaaatcaca aattgtaata tcaattacaa
     5641 gaactttctg aggtatagac atgaaaaaaa tacttattgt ggatgatgaa aaaccgattt
     5701 ctgacattat taagtttaat ttgacaaaag aaggttatga cattgttaca gcttttgatg
     5761 gacgcgaagc ggtaacaatt tttgaagaag aaaagccaga tttaattatt cttgatttga
     5821 tgctccctga gttggacggt cttgaagtag ccaaggaaat tcgtaaaacc agtcatgtcc
     5881 cgattattat gttgtcggct aaagatagtg agtttgacaa ggttattgga cttgaaattg
     5941 gggctgatga ttacgtgacc aagccctttt ctaatcggga attgctggca cgtgtcaagg
     6001 ctcatctgcg tcgtaccgaa actattgaaa cggctgttgc agaagaaaat gcttcttcag
     6061 gtacacagga actaaccatt ggtaatttac agattttacc agatgcgttt gttgctaaaa
     6121 aacatggtca agaggtagag ttgacccatc gtgaatttga actattgcat catctagcta
     6181 accatatggg tcaggtgatg acacgagaac acttattgga aattgtttgg ggatatgatt
     6241 attttggcga tgtgcgcacg gttgatgtga ctgttcgtcg tctccgtgaa aaaattgaag
     6301 acacaccaag tcgtcctgag tatattttaa caagacgtgg tgttgggtac tacatgaaat
     6361 cttatgacta gagatatcat tggaaactta tccacatttg aattagcgat ccttatctta
     6421 cttgtttttg ttgcttttta ctttatccat cttgcggtgc gtgattaccg aaatgcacgt
     6481 attattcgga tgatgagcca taaaatccga gacttgatta atggtcgcta tactgatata
     6541 atcgacgaaa aagcagacat tgagttaatg gagctttcag accagttaaa tgacctatca
     6601 gatgtttttc gcttgacgca tgaaaatctt gcccaagaaa aaaatcgctt ggcaagtatt
     6661 ttggcttata tgtcagatgg tgtacttgct acagaccggt ctggtaaaat catcatgatt
     6721 aacgagacag ctcgcaagca attaaattta agtaaagaag aggcactaaa gaaaaacatt
     6781 acagatttgt tagaaggtga tacttcatat acctaccgtg atttggtatc taaaacacca
     6841 gtggtaactg ttaatagccg aaatgatatg ggtgagtttg tctcattacg cttgcgcttt
     6901 gcgttgaata ggagagagag tggttttatt tcgggcttgg ttgtggtgct ccatgacacc
     6961 acagaacaag aaaaagaaga acgtgaacgc cgtctttttg tctctaatgt aagtcatgaa
     7021 ttaaggaccc ctttaacttc ggttaaatcc tacttggagg ctcttgatga aggtgcactt
     7081 aaagaagata ttgctccaag tttcataaaa gtttctcttg atgaaactaa tcggatgatg
     7141 cgtatgattt cagatctttt aaacctgtct cggattgata atcaagtaac ccaattagca
     7201 gtagagatga ctaattttac tgcttttata acttctattt taaacagatt tgatttggtt
     7261 aaaaatcagc atacaggtac aggaaaagtc tatgaaattg taagagatta ccctattacc
     7321 tctgtctgga ttgaaattga taatgataaa atgacacagg ttatcgagaa tattttgaac
     7381 aatgccatta agtattctcc agatggtgga aaaatcacag tccgtatgaa aacaacagat
     7441 acccaattaa ttatttccat ttcagaccaa ggactaggta tccctaaaac agatttgcct
     7501 cttatttttg atcggttcta tcgtgtagac aaggcaagaa gtcgtgccca aggagggacc
     7561 ggtctaggcc ttgccattgc taaagaaatc atcaagcagc accatggctt tatctgggct
     7621 aagagtgact atggtaaagg atcgaccttt actattgtct tgccttatga aaaagatgca
     7681 gccatctatg aagaatggga ggaagatgta gactaatcaa tgaatgagag tggttttaaa
     7741 tatagtattt tagcatctgg gtccacagga aattgttttt atttagaaac acctaaaaaa
     7801 cgcttattga ttgatgcagg gcttactggt aagaaaatta ccagtcttct tgctgaaatt
     7861 gaccgcaaac ctgaggattt ggatgctatt ttgattaccc atgagcactc tgaccatatt
     7921 aagggtgtag gcgttatggc ccgcaaatac catttggata tctatgctaa tgaaaaaaca
     7981 tggcagctga tggatgagtg caatatgcta ggtaagcttg atgcctcaca aaaacatatt
     8041 ttccaaaggg ataaagtttt gacctttgga gatgttgata tcgaaagttt tggagtcagc
     8101 catgacgcta ttgatcctca attttatcgg attatgaaag ataataagtc ttttgtgatg
     8161 ttgacagata caggatatgt cagtgatagg atgacaggta ttattgaaaa tgcagatggc
     8221 tacttgattg aatccaatca tgatattgaa attttgagat caggctctta tccttggagc
     8281 ttgaagcagc gtattttatc tgatttagga catttatcaa atgaagatgg agcgggagcc
     8341 atgattagaa gcctggggta caataccaaa aaaatatacc taggtcatct gagtaaagaa
     8401 aataacatca aagagttagc gcatatgacg atggtcaatc aactggctat ggcagattta
     8461 gcagtaggta cagactttac ggtccatgat acctctccag atactgcttg tccattaact
     8521 gatatttgag ataagaaact aaaagagaat atgcaaagta gcgctgattg gtgatatacc
     8581 agtcagggct atttttttga tgtcttaaag cgtattgtct tctgagttca atgaactctc
     8641 attggtcatc ctttagagca tcaatagact taaattagcc tgtagttttg gcttgtctga
     8701 tctatttatc aaaggtgttg aggattataa agataaggta gttttttagt tgttcagact
     8761 aaaaacaaaa tagctctata ctatctgtag tgggaaattc ccctcagata gtgtagagtc
     8821 ttttgattgt atgagaaagt ttcctttgac gggtatttaa gatgtttaat aaatcacgaa
     8881 aaatccactt agagtcggtt ttctaggtgg atttttggta tactaaataa ggtctactga
     8941 caaatatgaa agggattttt tatgaaacag cttgaagagt tactctcaac atcttttgat
     9001 attcaattta atgatttgac tttactagaa acagctttta cccacacctc ttacgctaac
     9061 gagcatcgtc tcctaaacgt ttcacataat gaacgcttgg aatttttagg agacgctgtt
     9121 ctacaattga ttatttcaga gtatttattt gcaaagtatc ctaaaaagac agaaggagat
     9181 atgtctaagt tacgttccat gattgtacgt gaggaaagct tggcaggttt ttcacgattt
     9241 tgttcttttg acgcttatat caaactagga aaaggtgagg aaaagtcagg tggtcgccgt
     9301 cgtgatacta ttttaggaga cttatttgaa gcctttttag gtgctctttt attagataag
     9361 gggattgatg cagtacgtcg cttcttgaaa caagtcatga tccctcaggt ggaaaaaggg
     9421 aattttgaaa gggttagaga ctataaaact tgtcttcaag agtttttgca aacaaaaggt
     9481 gatgtggcta ttgattatca agtcattagt gagaaaggac ctgcccacgc taaacaattt
     9541 gaggtatcga tagttgttaa tggtgctgtc cttagtaaag gtttaggtaa gtctaaaaaa
     9601 ttagcggagc aagatgctgc taaaaatgcc cttgcccaac ttagtgaggt ttagatgttt
     9661 ttaaaagaaa ttgagttgga agggttcaag tcctttgcgg acaagactaa aattgaattt
     9721 tataagggag taactgcagt cgtaggacca aatggttcag gtaaaagtaa tattacagaa
     9781 agtttacgtt gggctttagg agaatcaagt gctaaaaatc tgcgtggggg caagatgcca
     9841 gatgtcattt ttgctgggac acaaaaccgt aatcctctaa actatgctaa ggtagcggtt
     9901 gttcttgata attccgatca ttttataaaa actgccaaga aagaaatccg tgtcgaaagg
     9961 catatttacc gaaatggaga tagtgactat ctgattgatg gtcgcaaggt aaggctccgt
    10021 gatattcatg atttgtttat ggatacaggg cttggtcgtg attctttctc cattatttct
    10081 caaggtcgcg tagaggaaat tttcaatagt aaacctgaag aaaggcgcgc catttttgaa
    10141 gaagcagctg gtgttttaaa atataaaaca cgtaaaaaag aaactcagat taaattaaat
    10201 caaactcaag ataatttaga ccgtctagag gatattattt atgagctaga cacgcagttg
    10261 gccccacttg aaaagcaagc taaagtagct aaacaatttt tagaacttga tgccaatcgt
    10321 aagcaactac aactagatat tctagtcaaa gatattgata tagctcaaga aagacagact
    10381 aaagatactg aagctttagc tgctttacaa caagacttag catcttatta cgctaagcgt
    10441 caatctatgg aagaagatta ccaaaaattc aagcaaaaaa agcaggttct tagtcaagag
    10501 tcagatcaaa cccaaacaac attattagaa ttaaccaagc taatagctga cttggaaaag
    10561 caaattgaat tggttaagct agaaagtggc caagaagcag agaaaaaggc agaagccaaa
    10621 aaacacctcg aacagttaca agaacaattg gatggttttc aagcagaaga aaagcagcgt
    10681 acagaacaat tattgcatat tgatcaacaa ctttgtgatg tcaaacaaca gttaaacgaa
    10741 ttgagcaatg cattagagcg tttttcaagt gatcccgacc aattaatgga aactctaaga
    10801 gaagagtttg tattattgat gcaaaaagag gcggccttat caaatcagct gacagcttta
    10861 aaagcccatt tagacaaaga aaaacaggct cgtcaacata aggctcaaga atatcagctg
    10921 cttgttacta agcttgatca acttaatgac gagtctcaaa aagctcaagc tcattataaa
    10981 gctcaaaaag agcaagtcga aatgttgctc caaaattatc aagaaggtga taaacgcgtt
    11041 caggaattag aaagagatta ccaactgaag caagaaagac tatttgact
//
LOCUS       DAUVUY010000063        10883 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.063, whole genome shotgun sequence.
ACCESSION   DAUVUY010000063 DAUVUY010000000
VERSION     DAUVUY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.063"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..759
                     /locus_tag="VOX58_001392"
     CDS             <1..759
                     /locus_tag="VOX58_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HES5878619.1"
                     /translation="EDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITTCLHD
                     MTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLEAATR
                     NEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIETQQA
                     IEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNFQNNW
                     LPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            892..1548
                     /locus_tag="VOX58_001393"
     CDS             892..1548
                     /locus_tag="VOX58_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HES5878620.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQKAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGISLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(1681..1920)
                     /locus_tag="VOX58_001394"
     CDS             complement(1681..1920)
                     /locus_tag="VOX58_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HES5878621.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(2085..2576)
                     /locus_tag="VOX58_001395"
     CDS             complement(2085..2576)
                     /locus_tag="VOX58_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HES5878622.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            complement(2598..2888)
                     /locus_tag="VOX58_001396"
     CDS             complement(2598..2888)
                     /locus_tag="VOX58_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HES5878623.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            complement(3061..3354)
                     /locus_tag="VOX58_001397"
     CDS             complement(3061..3354)
                     /locus_tag="VOX58_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878624.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            3552..4676
                     /gene="mutY"
                     /locus_tag="VOX58_001398"
     CDS             3552..4676
                     /gene="mutY"
                     /locus_tag="VOX58_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HES5878625.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFALYPFATPQKKMLETYLKQKNA"
     gene            4752..5340
                     /locus_tag="VOX58_001399"
                     /pseudo
     CDS             4752..5340
                     /locus_tag="VOX58_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(5392..5706)
                     /gene="trxA"
                     /locus_tag="VOX58_001400"
     CDS             complement(5392..5706)
                     /gene="trxA"
                     /locus_tag="VOX58_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HES5878626.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(5787..6287)
                     /locus_tag="VOX58_001401"
     CDS             complement(5787..6287)
                     /locus_tag="VOX58_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HES5878627.1"
                     /translation="MESYEHFYAKLSQPFRKKPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            complement(6291..8630)
                     /locus_tag="VOX58_001402"
     CDS             complement(6291..8630)
                     /locus_tag="VOX58_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HES5878628.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADRNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFSLVRVQEEQ
                     KVKNKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRHKHVKHFAYASQNAGGSGATIVTLG"
     gene            complement(8779..9324)
                     /locus_tag="VOX58_001403"
     CDS             complement(8779..9324)
                     /locus_tag="VOX58_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HES5878629.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            complement(9327..9635)
                     /locus_tag="VOX58_001404"
     CDS             complement(9327..9635)
                     /locus_tag="VOX58_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878630.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            9792..10694
                     /locus_tag="VOX58_001405"
     CDS             9792..10694
                     /locus_tag="VOX58_001405"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HES5878631.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTISSQNIYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHPFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNTISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            10705..>10883
                     /locus_tag="VOX58_001406"
     CDS             10705..>10883
                     /locus_tag="VOX58_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HES5878632.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIV"
BASE COUNT         3483 a         2156 c         1827 g         3417 t
ORIGIN      
        1 gaagatgatt ataccctaat tatcgtagac gtcccgatat acgaagaacg taacaataaa
       61 agttactata ttactatgcc tttaggcatt attgtaacgg aaaatgctgt tatcacaact
      121 tgtctgcatg acatgaccct tttcgatcat tttcacaacc gccgggtcaa aaatttctat
      181 accttcatga agacacgttt tgtttttcag attctatatc gcaacgccga gctctttctg
      241 actgccttga ggaccattga tcgccaaagc gaacgtttgg aagctcagct tgaagcagct
      301 actcgaaatg aagaacttat cgacatgatg gaacttgaaa aatccattgt ctacttaaag
      361 gcttccttga aattcaacga acgcatcgtc aagaaattat ccagctcaac aagctctctc
      421 aagaaataca ttgaggacga ggatttgcta gaagatacct tgattgaaac ccaacaggcc
      481 attgagatgg ctggcattta tgaaaatgtc ttgaatgcca tgactgaaac cactgcttct
      541 attatcaaca acaaccagaa cactattatg aaaacactgg ccttgatgac catggcttta
      601 gatataccaa ctgtgatttt ctccgcctat ggtatgaatt tccaaaacaa ctggttacca
      661 ctaaatggcc tagaacatgc tttttggtat attaccttga ttgctatgct acttagttcc
      721 tttgttgtaa tttactttat tagaaaaaaa tggttctaga gtggagcaag ctgtagaatg
      781 tagttaacac ctgacaaaac tctgtgtcaa acggctggct ccaaagtctc atttgacact
      841 agctaatatc attgacttac tttaactaac ccatataaag aagggattcc catggaatta
      901 caagaactta ccaaaaaaaa tcaagaattt atccacaccg ctacgaacaa attaatccaa
      961 gacggcaagt ctgatgaaga cattaagctg attctcgaag aagccattcc agctattcta
     1021 gaaaaccaga aaaaaggtgt caccgctcga aatctattag gcactccgac tgcttgggca
     1081 gcttctttta gccaagaccc tagtcaaaaa gcagcagaaa ctgacaaaaa caccaatcct
     1141 tggttgatgt ggctagatac ctctttgcta ttcatcggaa ttgttgccct tttaaatggc
     1201 atcatgacct tcttcaacac aaacgctaca gttacaggat tgatttcctt actggcactt
     1261 ggttttggtg gaggagcatc catgtacgct acttattact ttatttaccg tcatctcgga
     1321 aaagataaga gccttcgtcc aagttggttc aaaatcatcg cagccttatc attagccatg
     1381 ttgatttgga ttgccttgta ctctgcgact gcctttttac caacctctct taacccacag
     1441 ttgcctcctc ttgccttgct cattattggt ggtatttccc ttgctttacg ctattaccta
     1501 caacgcaaat acaacattca aaacactatg tcacctgtta acaaataaca atcatttcat
     1561 ccttgctgtc tacgcaagga ttttcgttta ctatcattct ctcttttaga aaaaactcac
     1621 ctatacgcct agactagtcc tagtcaacta aaaaacagtc cgaaatggac tgtttgttta
     1681 ttagtcttcg tttacgtaag gcataagagc cataacgcga gcacgtttga ttgctgttgt
     1741 tactttacgt tggtttttag ctgaagttcc tgttacacga cgaggaagaa tttttccacg
     1801 ttctgaaacg aaacggctaa gaagctcagt atctttgtaa tcaacatatt caattttgtt
     1861 agctgcgatg aagtcaactt ttttacggcg tttgaatccg ccacgacgtt gttgagccat
     1921 gtattttctc ctttataaat tatgatgtta agggctctta aaaaacgact gaattttaga
     1981 aaactggcaa gaaatcttga tttcctaggg agttgatcta aattctaagt tctaaatttt
     2041 tagcccgaac tcaaataatt tcgtattcgt tcaatccatt ctgactagaa tggaagatcg
     2101 tcatctgaga tatccatcgg gtttgagttc ccaaatgggc tatcatctct tccaaagtta
     2161 ggcgtttgtt gtgcaggcgc tgagtaactg tttgatgatg aagtgttatt gttaaaacca
     2221 ccattaaatg agccagttga gccaccttca cgtgtagcac gactttccaa catttggaaa
     2281 ttatctgcaa caacttctgt tacatagaca cgttgtcctt gttggttttc gtagttacgt
     2341 gtctgaatac gacccgtaac tccgatcaag gcaccttttt tagcccagtt cgctaaattt
     2401 tcagccggtt gacgccagat cacacagtta atgaaatctg cctcgcgttc accattttgg
     2461 cttttaaagg tacggttaac agcaagtgtg aaggtagcca cagctacttg acttggtgtg
     2521 taacgaagtt ctgcatcctt ggtcatacga ccaactagta ctacattatt aatcataaac
     2581 taccctctta gttggaatta agcgtcaagt ttaacgatca tgtgacgaag aatgtcacca
     2641 ttgatttttg aaagacggtc aaactcgtta agagctgctg cgtcagtcgc ttcaaggtta
     2701 acgatgtggt aaagtccttc acggaaatcg ttgatttcgt atgcaagacg acgtttttcc
     2761 caatcttttg attcaacaac agttgcaccg ttgtcagtca agatagagtc aaagcgtgct
     2821 accaaagcgt ttttagcttc ttcttcaatg tttggacgaa tgatataaag aatttcgtat
     2881 ttagccattg atattttcct ccttttggtc taatgactca ctacttttgg agtgagtaag
     2941 tgaggtgtac tcacagaatc ttattgtaca ctaatagtca attaaaagca aggaactttt
     3001 tctacttgac taagttttgt caaacatttc ctttcacgag ttttaaaaat tggcacttag
     3061 tcagactcct aaaaaatagg tgaaaaaaga aaaagcaagg aaaagtttta accaaagtgc
     3121 aaaatataaa attagggcca aaacaaaaag gttaaacttt ttaaaaataa tagcaaccat
     3181 ggcacatacg atacctaatc caggaaaaac ttgcgtcatg atcagccatt ccaactgtag
     3241 tccgctcaaa gaaggatcaa atggataaat atagttaaga aataacacaa gaatcatcat
     3301 tataaaaacg aaccacgaat accgatcaat aagattcttt aatttctgga acatgtcccc
     3361 tccaaattaa aacctgtctg cttctatcgt taatttcagt tattctaaat caacatttag
     3421 atgaggtaaa cctaatatta ttataatatt tccttttctt tttcgcaaat acctaaaatc
     3481 tttttttact ctttttaagg ctcctgtgtt acaataacaa catgattgaa ttaaaagatt
     3541 atggtattaa tatgtgggat aatgagacaa tcgcttcttt tcgtcgcaca cttttagggt
     3601 ggtatgacca agaaaaacgt gatctccctt ggcgaagaac tactaatcca tattatattt
     3661 gggtttctga aatcatgttg caacaaacac aagttaatac ggtaatccct tattataaac
     3721 gttttctaga gtggtttcct caaataaaag atttggcaga cgctcctgaa gaacaattac
     3781 taaaggcatg ggagggacta ggctattatt ctcgggtgcg taatatgcaa aaggctgcac
     3841 agcaagtgat ggttgatttt ggtggcatat ttcctcacac ttatgatgat attgcttcat
     3901 taaaagggat tggtccttat actgcaggtg ctatcgccag catctcattc aacttaccag
     3961 agcctgctgt tgatggcaat gtgatgcgtg tcatggctcg tttgtttgaa gttaattatg
     4021 atatcggtga ccctaaaaat cgtaaaattt ttcaagctat tatggaaata ctaattgacc
     4081 ctgacagacc cggtgatttt aaccaggcac ttatggatct aggtacagac atcgaatccg
     4141 ctaagacacc taggcctgat gaaagcccta ttcgtttctt taacgctgct tatttgaatg
     4201 gtacttatgg taaatacccc ataaaaaatc caaagaaaaa gcctaaacca atgcgaattc
     4261 aggcttttgt cattcgtaac caaaatggcc aatatctact ggagaaaaat actaaaggga
     4321 gacttctagg aggtttttgg tcatttccta ttatcgaaac gtcacctctt tctcaacaat
     4381 tagatttatt tgatgacaat caatccaacc ccataatctg gcaaacccaa aatgaaactt
     4441 tcgaaagaga atatcaatta aagccccaat ggactgataa tcactttcca aatattaagc
     4501 acacttttag tcaccagaag tggacaatag aactcataga gggggtggtc aaagcaacag
     4561 atttacctaa tgctcctcac ctaaagtggg tagcaattga ggactttgct ctgtatccct
     4621 ttgcaacacc gcaaaagaaa atgctagaga cctacttaaa gcagaaaaat gcttaatcgc
     4681 aagtgaacta taacggttca ctttttgttt acaattcctt tacgccgctg taaagttttt
     4741 cggtgatatt tatggaaatt ggtcagcaaa ttattcgcta ccgtaaacaa caagcgctct
     4801 cgcaagaaaa attagccgag aaagtctatg taagccgtca gagcatatct aactgggaaa
     4861 atgacaaaac ttacccagac atccatagcc tcttactcct aagtcaaatt tttcaagtga
     4921 gcttagacca actcattaaa ggagacattg agaaaatgaa atataccatc actcaggttg
     4981 ataaaaagaa ttttaaacga gataccaaag taatggtaac attgatgata ctattaatga
     5041 tttcaagtta tccattaagt ctatttctta gagtggctag gacttggcat ttttgtttta
     5101 ttatcgataa ttactatgac ttatgctaat cgtgtggaac gttttaagaa aaaatatgat
     5161 gtgcaacctt ataaggaaat cctagctatt tcaaatggaa aattattaga cgaaatcgaa
     5221 aaacgagaag aaagagcaaa acttccttat cagaaacccc ttattgtcac tgtatttttc
     5281 ctcattactg ttgcttttgc ttttgcaagt cgttttatgt ttacatggtt attccattga
     5341 agttagtagg tcataaaata aagctcagcg tgacgctggg ctttatttta gttaactcaa
     5401 ttcagcaata atagctttca gttggtcttt agtatgaaca cctgcaactt gtttaaccac
     5461 ttctccgtct tttttaaaca tcaatgttgg aattgacata ataccaaact gacgagcagt
     5521 ttccggattt tcatcaacat ccattttcaa aattttgagt tcatcttcat cgatttcttg
     5581 agataactgt tctaagattg gtgcttgcat acgacatgga ccacaccaag ttgcccaaaa
     5641 atcaatgagt actaaacctt ctttagtttc ttctacaaat gtagcatctg ttacttctaa
     5701 tgccatcttt tttctccatt tcatcttaat gataagccta ttgtacaaga atttagttgt
     5761 ttttgaaact atttgctacg aactcgctaa atcaataaac aaagccctaa catcaagcca
     5821 ataaggtaac ctacaataac atccttagga tagtgaatac ctgctatcac acggcaaatt
     5881 gccaataaaa ccgataatat taaacaaact attccaaagt aaacataata tcgtaacaaa
     5941 cacatactaa tcatcgtagc tgaaaaaaca tgtctgctag gcattgacct tccctttgta
     6001 tccttatcaa ttaaaggttt aatattccat ttttcatacg gtcgtggaaa atcaaagcgt
     6061 ttcctaatgt aagacaaagc tataaagcca acagcaggaa taattaataa ccttaaccat
     6121 aattgaaatg tcatcccttg ccaaagcgta aatatcagaa aagatggata aagtatgtac
     6181 atcactcctg tgattatttt tagtaaaaca tttaaaagaa ttattaactg tggttttttt
     6241 ctaaaaggtt gagatagttt ggcataaaag tgttcatatg attccatcat ttaccctaac
     6301 gttacaattg tggcgccaga tccccctgca ttttgtgagg cataagcaaa atgcttaaca
     6361 tgcttatgac gacgaagata ttttgtcact ccctcacgaa taacgcctgt accaatacca
     6421 tgaatgatat caacttgtcc catattgtta agcaatgctt gatcaataaa atgatctaac
     6481 tcttgcattg cttcttcgta tcttttacct ctaagatcaa gtcgagctct tggtccagaa
     6541 ctatcagcct ttttaaccac attaatctgt ttatttttga ctttctgttc ttcttggact
     6601 ctaacgaggc taaattcatc ttgtgttaat gtcattttga taattcccac ttgtgcttcc
     6661 caacggccat cttttaattg actagttaag gtacctcgct gtccataact agtcactata
     6721 atatcatcac caattctagg ggcacgagct gctttgattt ttttagcctt atttaagact
     6781 ttattttttg ataaatcaac ttgaggtgct aattttttta tttgtgcctt agcatctata
     6841 atttcgtgag gttttaattg gctcttatca ttgagttttt ttaagatagt atcactctca
     6901 ttcaaagcca tatctacaat ttcttgagct tcttgataga ttttttctaa ctctttatcg
     6961 cgttcatgtg aaaattcatt atagagtttc ttaaccgcac gattgaattt gagattttct
     7021 tgttcaactt ctttaatatg atccagtctt ctacgtgtct caagtgtctg tgcctctaac
     7081 tgttcaataa tacggttaac atctgagtca gaatctgtca tctgcttagc ttgtttaaca
     7141 ataaatggag ctaaaccaag gcgagaagca atttcaaatg catttgatcg tccaggaact
     7201 ccttgcataa agcgatacgt ggggctaagc gtttcggcat caaattccat gctcgcattc
     7261 tctacaaaat ttgtctcaat cccataagct tttaattctg gatagtgcgt ggtcgccatc
     7321 gttttgatat gacttaacct aaggtgttct aaaatagcca tagccaaact agcaccttct
     7381 tgaggatccg ttcctgctcc tagttcatca aagagaacta aactattgcg gtcagcctcg
     7441 tttaaaatac tgactatgtg cgtcatatga ctagaaaaag ttgatagact ttgttcaata
     7501 gattgctcat cgccaatatc tgcaaagata ttgttaaata ctgcaatttt actaccttta
     7561 tccgctaata ctggcaaacc agactgtccc attaattgtg ctaaaccgag tgtttttagc
     7621 ataatcgtct taccaccagt attgggacca gtgatgacaa ttgcagttaa atcgtgatca
     7681 aaatgtaagt cattagccac aggattactt aaaagaggat gacgaacatt gattaatgct
     7741 aacgtgctgt catcagagat cttaggtatc gtcgccttat tatcagacat aaaaagatat
     7801 ttagccctta caaaatcaag atgcccaaga atccatgcat tatttctaat agtggcgaca
     7861 tgaggtctta acaagtctga aaatgcgtgt aaaatacgac cttcttcgtg gcgttcgtca
     7921 gctctaagct gagttatctc ttcgtttagt gtaactacag cacgaggctc aatataaaca
     7981 gtacttcctg aagaagagat gtcatgaacc acaccagaaa tacgattccg ataagtattt
     8041 tttactggta ggacacttcg tccactacga ctagcgatta gattctctga taaaagctct
     8101 gctttttcct taagcatatc ctgtaaaatc tgacgaaccc gtcgttcact atttgttaat
     8161 tgacgacgga tacgctctaa ttctggactc gcaaaatgtt ctaaaaaacc accatcattg
     8221 atagcttgaa aagacccttg cagattaggg aattgttcca aattttcaaa caaacgatcc
     8281 aaagattgga aagaaacatt atccaaatca gaataaaagt gaatcatatc cgaagaactc
     8341 tgtaaaactt ttttgatagc caaaagttct tgaatattaa gatcagctga aagctctaag
     8401 cgttttaaac tctcagagat tgaacttagg ctgactatgc caaatgagtg atgttcaaca
     8461 aaaatctgtt ccatgtcaga aatttcatta aaactttttt ctattttagg agcctccgtc
     8521 atcggctcca gctctaataa ttcttcttgt gattgttctg tcttgagata aggtaatagc
     8581 aattccttaa ctttgttaaa ttctaactgc tctaaaatct tgttattcat actcttcatt
     8641 atacctaaaa tgatggaaaa tgtggcaaga aagtcactaa gttcccatgc gttctcttta
     8701 tttctaaaca gtttctcgta aataaagaac ctaaaggtga aactcactcc aggttcttat
     8761 attagtcaca aaacgatatc aaataatggc ttgaatccac aatttttgaa taattattgt
     8821 caatggtgga aggtgttcaa tcaccaaacg agcaagaaaa ctagagtgca aatagtgctg
     8881 taaaaaaggc atagggaccg ttgcaaaaat agacagtaac atgttcaaaa aaagaagcga
     8941 tactagaagc gctagtccgc cactcaaaca ttttgtccac tggttatcaa aataatctag
     9001 caaaaggaaa tgaacaaaaa tcccaagaaa gcgactcagt gcatacccca ataagaaaat
     9061 gataaaaaag gcaaggcctg cataataaac cttatctaaa acaaagatat caactgattt
     9121 aaagaaaaag actgatgttc cttctaccgg gttagaataa ggaatccaaa gcgttagctt
     9181 atgggctaat ccaatataaa atcgattagc taccaaaaga gaaagtaggg ctcctaagac
     9241 ataaaacgat tgaagaataa tacctctact ataaccaata taaaaattcc aagttaaaac
     9301 aagcactatc aataaagata acatgattaa acctcattca catctatttg attagctttt
     9361 tcttgaagac caaccaaggt tttttgacgt aaatctaaaa tttcagcttc cattttttca
     9421 atggcaatct ctctgcttaa ttgtgttgac aaggtattga tagccattaa aatagcgatt
     9481 gtctcgtcat cagcttctgg taaatgattt ttaagagcct gatacttttc tttagctacc
     9541 cgctcaactt cttccataaa gagattgtct ttgtcagttg tcaatgttaa tgttttttca
     9601 ccaaaggtga atttatagcg attaatactt ttcatagggt cacctcatta gcattatacc
     9661 gtaaaatgaa actttcgtaa aatagtcaac accattgccc taacccgtct aaacattcga
     9721 gtctctgttt tctttgttaa ataaacttat gaattaacca tagtcaacta ttttatgtta
     9781 aaataattat tatgaatacc ttagttttga aaatagatgc tatactgagt aaacatttaa
     9841 aaaaacagct agccccctac accatcagta gccaaaatat ctatgtagcc tttgctgcta
     9901 aaaaaaatgg ggttactgtt ttgctgtaca aatccggaaa attagttctc cagggaaacg
     9961 gtgcaaatgc tttagctcaa gagcttaatc ttccagtagc taagacagtt tttgaagcaa
    10021 gcaacaatag ccaagatata cctataatcg gcagtgatga ggtgggtaat ggctcttatt
    10081 ttggtgggat tgctgttgtg gcaagttttg tcgatcctaa agatcatccc ttcctgaaaa
    10141 agcttggggt tgatgattct aaaaaattat cggataaaac cattcaacag attgcgcctc
    10201 tgttagagaa acaaatacct catcaatcac ttctcctatc acctaaaaaa tataacgaat
    10261 tggttggtaa aagtaaacca tataatacca tttctatcaa agttgctctt cataaccaag
    10321 ctattttttt gttgttacaa aaaggcattc aacctaaaca aattgttatt gatgctttca
    10381 ctagccaatc aaattatgaa aagcatctaa aaaaggaaaa aaaccatttc ccaaatcctc
    10441 taacctttca agaaaaggca gaaagtcact atttggctgt ggctgttagc tctatcattg
    10501 ctagaaatct atttttagac aatctcgatc aattaggtca agatttaggt taccaactcc
    10561 caagcggtgc tggttcagct tcagataaag ttgctagcca actcttagcc gcttatggta
    10621 tgtctagctt agaatatagt gctaaacttc acttcgccaa cactcacaaa gcgcaagcgc
    10681 ttctcactaa ataaaaggaa tcatatgaaa cagtttatta aagaatgggg cccattcact
    10741 ctctttttaa ttctctttgg tctatctcgt ctttttttgt ggcaggctgt taaagtagac
    10801 ggccattcta tggacccaac tctagctcat ggcgaacgcc ttatcgtttt taatcaagct
    10861 aggattgatc gctttgatat tgt
//
LOCUS       DAUVUY010000064        10741 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.064, whole genome shotgun sequence.
ACCESSION   DAUVUY010000064 DAUVUY010000000
VERSION     DAUVUY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.064"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(26..673)
                     /locus_tag="VOX58_001407"
     CDS             complement(26..673)
                     /locus_tag="VOX58_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HES5878633.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(676..1335)
                     /locus_tag="VOX58_001408"
     CDS             complement(676..1335)
                     /locus_tag="VOX58_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HES5878634.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVTLIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQVREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(1347..2531)
                     /locus_tag="VOX58_001409"
     CDS             complement(1347..2531)
                     /locus_tag="VOX58_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HES5878635.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLVDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            2652..3563
                     /locus_tag="VOX58_001410"
     CDS             2652..3563
                     /locus_tag="VOX58_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HES5878636.1"
                     /translation="MNISSLAYFIEIVNCDFNLTKAAQKSHISQPALSSYIKTIEKEE
                     GVVLFQRQKGRLVGLTSVGERFFENAKVVIASHEQLMADLRSESKKYKGEISIGIPQL
                     ISGIVFSELIPKLIEENPEAKFHIVETGAYELQKKLFLKEVDFAILLTPTTLDATIAD
                     IDILLRDQLCLYMSHKTFQETFDSNPNISIKALHRQNLVTFDKTFMINHQLTTLFEEA
                     NSLPVVKCYSASWDFLLNCTRYSSYLTILPRPITHFAHMDGLVEVQLTEHPKWEVVLA
                     SLKHNKTSHLKHYIKHTILDYFMNQRF"
     gene            complement(3633..5039)
                     /locus_tag="VOX58_001411"
     CDS             complement(3633..5039)
                     /locus_tag="VOX58_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HES5878637.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSIVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
     gene            5392..6057
                     /locus_tag="VOX58_001412"
                     /pseudo
     CDS             5392..6057
                     /locus_tag="VOX58_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6323..9817)
                     /locus_tag="VOX58_001413"
     CDS             complement(6323..9817)
                     /locus_tag="VOX58_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HES5878638.1"
                     /translation="MTQKNSYKLSFLLSLTGFILGLLLVFIGLSGVSVGHAETRNGAN
                     KQGSFEIKKVDQNNKPLSGATFSLTPKDGKGKPVQTFTSSEEGIIDAQNLQPGTYTLK
                     EDKAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISKAGSKDVSSSLQLEKSTMSV
                     FSKYGGTEVTSGAADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDKRLNPKGI
                     SQDIPKIIYDSENSPLAIGKYDAKTHQLTYTFTNYIAGLDKVQLSAELSLFLENKVVL
                     ENQTISDFKSTIGDQEIKYGGTVNVLYGNASGQDNNYVTNGLSNVGGSIESYNTQTGD
                     FTWYVYVNPNRTNIPYATMNLWGFGRLVKSNNSTSKSEDSATSSAQLEKVEIYEVKED
                     YTLPTSYGVDLTKLNLRKDLEAKLPQDSTQNSNKRLRIDFGDKLQNKAFVVKITGKTD
                     QSGKPLVVQSNLASFNNETAVTYGNPVNNVYFQNEIALSPSKGSGSGTSEFTKPSITV
                     ANLKRVAQLRFKKMSTDNVPLPEAAFELRSSNGNSQKVEAKSNTQGEVHFKDLTSGTY
                     DLYEKQAPKGYQQVTEKLATVTVDTTKPAEEMVTWGSPHSSVKVEDSKEVTIVNHKET
                     LTFSGKKIWENDRPDQRPAKIQVQLLQNGQKMSDKVQEVTKDNDWSYHFKDLPKYDDR
                     NQEYKYSVEEVKVPDGYKVSYLGNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGSKI
                     IDEDTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEFKN
                     LKKYNGTGNDIIYSVREVTVPTGYDVTYSANDIINTKREVITQQGPKLEIEETLPLES
                     GASGGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGATM
                     ELRDSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQVTV
                     NGKATKGDAHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDLIIGG
                     QGEVVDTTEDTQSGMTGHSGSTTEIEDSKSSDVIIGGQGQVVETTEDTQTGMHGDSGC
                     KTEVEDTKLVQSFHFDNKESESNSEIPKKDKPKSNTSLPATGEKQHNMFFWMVTSCSL
                     ISSVFVISLKTKKRLSSC"
     gene            10202..>10741
                     /locus_tag="VOX58_001414"
     CDS             10202..>10741
                     /locus_tag="VOX58_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HES5878639.1"
                     /translation="MVIFDLKYVQTLHSLSQLPISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQAAQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQ"
BASE COUNT         3238 a         2273 c         1792 g         3438 t
ORIGIN      
        1 tatggcaaga aaaggctact agtcattaac cttcagctat gcttagaggt acacctgtcg
       61 cagcttgaac ctcatcaaaa gtaaaccctt cactgatttc aattacttgg ataccatctg
      121 gcgttacttt aaacacaccc atttctgtga taataaggtc aaccacacct tttgctgtta
      181 aaggtaaggt acattctttt aggagcttat gctttccttt attggtatgc tccatagcta
      241 cgatgacttt tttagcacca actaataaat ccatcgctcc tcccatacca ggaaccattt
      301 tgcctggaat caggtaattg gcgatatctc cattttctgc aatttctaaa gcacccaaga
      361 cagtcaagtc aacatgacca ccacgtataa tgccaaaaga atctgcacta ttaaagaaag
      421 cggctcctgg ataaaccgtc acaggctgtc ctcctgcatt aacgatagtc gaattcggat
      481 catctgactt aggacctagt ccaataaacc cattttcaga ttgaaaatac actgtcattt
      541 cttcaggcac aaaatttgct actttggttg gtagaccaat acctaaattt accaaagtac
      601 catcttctaa ttctttggcc acacgctttg caatacgatt ttgaatctct tctttactta
      661 atgcaaccgc cataactata ctccttcttt aacaatgtaa tctacaaaaa tgtgtggtgt
      721 atgaacgcat tctggttgta tggtgccgac tggtactatt tctcgcacct gaacaattgt
      781 tgtttttgca caagctgcca tcaattgatt aaaattattt tcagaaccac tatactgtaa
      841 atttcccatt tcatcagctt tattagcaaa tatcaaggtg acatctgctt caaatgggta
      901 ttctaagaga taggtttttc ccttgatggt ttttgtttcc ttgccttctg ccactaaggt
      961 tcctacccct gttggcgtgt aaaagccacc aatgccataa gctccgatgc gaattttttc
     1021 ggcgaaggtt ccttgaggaa tgagctcaat cgtcgtttca ccggcagtca tttggcgccc
     1081 agcttcttta tttaatccaa tatgtgttgc ataaatggtt ttgaattgat gattagccac
     1141 catttttcca acaccgcgat caacaaaacc agcatcatta cagatcaagg ttaaatcttt
     1201 tgtgcctttg tcaaccagtg catcaattaa cgcttctggt gttccattag ccataaaacc
     1261 accgaccata atcgtgtcac catctttaat atgactgata gcttctgcta ttgctattct
     1321 tttattctct ttcatacggt taccttctat cttgttacga tgatactttg accttggcca
     1381 ccaccaatac aaagcgttgc taagccatgt ctcacatcac gcttagccat ttctgctaat
     1441 aaggtgacta aaatacgagc acctgatgct ccaatcgggt gacccaaagc aatagctcct
     1501 ccattgacat taactttttc attgtcaaaa ccaaggtctc gtgaaaccgc taaagcctga
     1561 gcagcaaaag cttcgtttgc ttcaatcaaa tcaatgtcat caattgtcaa ctgagccttt
     1621 gcaagagcct ttttcgtagc aggtattggt ccgcagccca taatacttgg gtctacacct
     1681 gcacttgcat aactagtgat tttagctaaa ataggaagcc ctaattcttc agctttttct
     1741 ttactcatca ataaaattgc tgctgctcca tcattgattc ctgaagcatt accagcagtt
     1801 actgtcccct catctttgat aaaagcaggg cgtaactttg ctaacttatc cactgtcgtt
     1861 ccaaatttag ggtattcatc ttgatcaacg agtaaaggtt cacctttacg ctgaggaata
     1921 gtgacaggtg caatctcttc tttgaaacga cctttttcta tggcaagttg ggctttacgt
     1981 tggctatcag ctgcaaaggc atcttgttct tctctggtca gtccatactg ttgcacaata
     2041 ttttcggctg ttatacccat gtgataacct gcaaaagcat cactcaaccc atctttaagc
     2101 atggtatcta ctactttact atctcccata cgagatcccc aacgttggcc ttgtaaaacg
     2161 taaggtgctt gactcatgtt ttcggtacca ccgactaaaa caatatcagc gttgcccgtt
     2221 tgaatctttt gagcacctaa ctcaactgct tttaagccag aaccacagac catatcaatc
     2281 gtaaaagcag gggtatcata agaaagcccc gcatttagcg ctacttgccg agcaacattt
     2341 tgacccagac ctgcatgtaa cacattccca aagatcactt catcaatggc atcactttta
     2401 agaccggtat cagctaaaat ttttgtaaca actgtcaccc ccaaatctac ggctgataaa
     2461 gatttaaaga ctcctccaaa atttcctatc ggggtacgat aagcagatgt gatcaccact
     2521 tctttagtca tagaaacttc tcctttttca ttattgaaac gcttacattt ttattatata
     2581 atgtgaacta taataaacaa atatcgtttt tttatatatg ctataactaa acgttatcga
     2641 aagaggaaaa tatgaatatc tcaagcttgg cttattttat tgagattgtt aattgtgact
     2701 ttaacttaac caaagcagcc caaaaaagtc atatttcgca acccgccctt agtagttata
     2761 tcaagactat cgaaaaagaa gaaggagtcg ttttattcca gcgtcaaaaa gggcgtctag
     2821 ttggactaac ctcagtcggc gaacgctttt ttgaaaatgc taaggtggtt atcgcatcac
     2881 atgagcaatt aatggctgat ttgcggagtg aatccaaaaa atacaaagga gaaatttcaa
     2941 tcggtattcc tcaactcatc tctggcattg ttttttctga gctcattcca aaactcatcg
     3001 aagaaaatcc cgaagctaaa tttcatattg tagaaacggg tgcttatgaa ctgcaaaaaa
     3061 aactgtttct aaaagaagtc gattttgcga ttttattaac acccactaca ctagatgcta
     3121 ctattgctga tatcgacatt ctcctaagag atcaactctg cctttatatg agtcacaaaa
     3181 cctttcaaga gacgtttgat agtaatccta acatctctat caaagcatta catcgtcaaa
     3241 acttagtaac ttttgataaa acctttatga ttaatcatca gcttaccacc ttatttgaag
     3301 aagcaaatag tcttcctgta gtcaaatgtt attctgcctc gtgggatttt ttattaaatt
     3361 gcacgcgcta ttcctcttac ctaacaatcc taccaaggcc tattactcat tttgctcata
     3421 tggacggttt agtggaggtc caactgaccg aacatccaaa atgggaagtt gttctagcct
     3481 ctctcaagca caataaaaca agccatttaa aacattatat caagcacacc atcttagatt
     3541 atttcatgaa ccagcgcttc taattgcttg actcagacct taaaaaaaaa cgcctgaagg
     3601 aaacaatccc aagcgtttca tgtagttatt tcctaaaata caaataggta aatgatggca
     3661 atacctgtga ccaccaagac acaaggaata gtataagcca caatttgatt gaattttagt
     3721 ttgaagtttg agagataaga taaggcccaa aaaggaacta tcaagtttgt tgtggcatct
     3781 cctaattgat aagcctgcaa aatcttacct agatcattac cgacttcgat agttgcaggg
     3841 acgatatatg gagcttcaat cacaaatttt gagcctcctg atggtaccgc aatattcagc
     3901 aaagccgagt aaagataagc aaagaccggc catgttgtcc catttgaaat agcgacaaag
     3961 aaatgggaaa tctctagtcc tagacctgta ctttgaatga ttccaaaaat accagcgtaa
     4021 aatggaaact gaagcaccaa gccccaagat gacatgaccc catctttgaa taaggaacca
     4081 taatattctg gtccttgttg accacaaaga agcaacccta agcctaaaaa taaaaagtta
     4141 aaactattga gatctagacc agacaagccc accaccgaaa aatgataaag gctatagcta
     4201 acaatggccc caccaacaag atacatcact aagcgagatg cgttcatctt ttcagcaggt
     4261 gtgtttcttg caataacaac atgtgaagcg gtatcaagac tatggtgata gatctcatca
     4321 tcaagttcca tgattttagt ttcatctttt gggtgcgcca ataaagcaaa gcaaagcggt
     4381 accagcatac acaccactaa aagactgata aatggaagat tcaaaactga ttctgtcaga
     4441 ggaacacttt caggaacaac ctgtttataa gcatctgcga cactatttcg caaataatct
     4501 ggagtagccg aataaagcac cgcagcacca gataaaccag aagtagctgg taaaaaggta
     4561 aaaaataaag tagcaacaaa caaaggtgta tgcaccttaa tccctttttg cctagcctga
     4621 acaagcaact ctttcccaaa cacaatggca accatcatac caagacccca gtgtaaaaag
     4681 ccaaagatag aacctactac aatagaaaaa ataaaaatct gagtcctggt ttgaggcagt
     4741 ttggcaaggg acttaaacat tttatgagca ggtggagaac tcgctacagc attccctgtc
     4801 gcaagtaaaa tagtcatctg catggcaaag gacaacagcc cccaaaaacc atttacccag
     4861 ccatagataa tcccgcccgt cttttccgta ccaataaagg gactatccgt catcaacaca
     4921 gacatagtaa cgactaaaaa ggttaaaata aaacagataa aaagcgattc tggcatccat
     4981 ttcataaagc catcaatata gcgatccata aacctttttt tggttttgat gttattcatg
     5041 ttgtcaattc ctttcacatt gtccacaagc acttttgttc ttttgatcag taacctatcg
     5101 ataatactaa taagcgtttc tttagttcat ttagttatca ataagtttaa acccacaaaa
     5161 aacacttttg ttcccttttt attatgacaa cgttttcata gacaaacaac tatgattttt
     5221 ttattgcttc cataaccctt atttatagca aaaagaatga cgcttcactc atcaacaatg
     5281 tctttgttat atttaaataa ttaaaaaatt atgataaaaa attaaaaaat aatttacttt
     5341 ttagtttttt tgatataata tattagtatt aaaagaaagg tttttcatga tatgaaacga
     5401 tgtaataaat atctcttcac ttcactacta gcagcttcga tgctgtttag ttcgtataaa
     5461 tcagtacatg cccatgataa cattgatgaa aaaggtaaag tgcaccttta ctggcaggga
     5521 aattactagg ttgataacta tgtcgactat actaaaacat tggtcgataa caataacagt
     5581 atcgaatgga ctgtaacctt taattcagct aaagaacaat gggtttaccc tgatttctca
     5641 gtgtttcttc ctaagggtgt aaaagctcct acggagataa cttatgagca tcattatggg
     5701 aacggtacag ttgattctaa aaaacacaat aatacacaat ggcattatga ttggggaagc
     5761 caacaaacaa attttaatca agaatttgat aaattccctg gttatactgg ctggagtcct
     5821 tctctagata aattttacaa actaaaaaac gacggaaaat tttctcacgt tttagtagac
     5881 acctacggcc gtcaatcgca cacttatttt tctcataaaa tggtttggaa gtttaaaact
     5941 gagcttgaag ataattacaa agataaatgg gataaattac cgtttatagc aggtattaaa
     6001 caaaacaacc cattagcagc atcattccca agttataaag gggagttcgg tgagtaaatg
     6061 aagctgccag tccatcatct tttgcataat tacagtcaga gtctgagaca acatgattgc
     6121 tcagactctt tttattatgc ttgaagtggc cgaaatactt tcgtaagtgc cccacaagat
     6181 taaaaaggca gaaaactttt taaggctctg cctttttgaa ctacttaaaa tgacttatct
     6241 agtgaaccga gacgatcgat tccagaaaaa gaggtccact aacaagtgga ccctttttcg
     6301 ctattgtcac caacagctta tcttaacatg atgataggcg ttttttggtt tttagtgata
     6361 ttacaaaaac actactaata agtgagcaag aagtaaccat ccaaaagaac atattatgtt
     6421 gcttctcacc agttgctggt aaactagtat tactctttgg cttatctttt ttaggaatct
     6481 cagagttact ttctgattcc ttgttatcaa agtggaagga ttgtactagt ttagtatctt
     6541 cgacttccgt tttacaacca gaatccccgt gcatgccagt ttgggtatcc tctgttgtct
     6601 cgacaacctg cccctgaccg ccaatgataa cgtctgaaga cttgctgtct tctatttcag
     6661 tagttgagcc agaatggcct gtcataccac tttgtgtgtc ttctgttgtg tcaacaactt
     6721 caccttgacc gccaatgata aggtctgaag atttactgtc ttctatttca gtagttgagc
     6781 cagaatggcc ttgctcgtct ggaagctttt cttcaatatc aataacctga cctgaaccct
     6841 tagttggctt gtaagcatca accatgacaa tatgagcgtc acctttagtt gctttgccat
     6901 ttacagtaac ctgaccttgc tcattaactg taaaggtaat agcagttgct atctcataac
     6961 cgtctggtgc tgcggtttcg acaaatgtat attttcctgg catcaggtag aaatctttta
     7021 cttgtccatc tgaaatccat gtactaatag ttttaccaga tgaatcacgc aactccatag
     7081 ttgcaccagc taactctttg ccgtcaatat cacgttttga gaatttaata tgggtagcac
     7141 tatcttcttc aattgtcata tcaccggact gaccttgctc acttgataaa cctgacaagg
     7201 tatcaactgg gcgtgagtct tcgacagtag tggtaccgcc tgaagcacct gattctagcg
     7261 gaagcgtttc ttcaatctct agtttcggtc cttgttgtgt aataacctca cgtttggtat
     7321 taataatatc attagctgag taagtcacat cataacctgt tggaacagtt acttctctaa
     7381 cagagtaaat gatgtcatta cctgttccat tatacttctt caagttttta aactcaaatg
     7441 accactcatt acctgagcca gaaataaatt tggtttgacc ttccacagcc actccatcag
     7501 cataaagctg cacttgaatg gcttggggac gattttctgc cgtatcattt ttccaaattt
     7561 tcttaccttt gaaagacgtt agcgtgtctt catcaatgat ttttgaccca gattgacctt
     7621 ttatttcagc atttccaaat tcaaggttaa agttattctg ttcaaacaca aattctgttt
     7681 ctctggtgtt aaatatatca tttcctaaat acgacacctt gtagccgtct ggaactttca
     7741 cttcttcaac tgagtactta tactcctgat tcctgtcatc gtacttaggc aagtctttga
     7801 agtgataaga ccaatcgtta tccttcgtta cttcttgaac tttatcggac attttttgac
     7861 cattttgcaa cagttgcact tgaatctttg caggacgttg atctggtctg tcattctccc
     7921 aaattttctt ccctgaaaac gtaagggttt ctttatggtt gacaatcgtg acttctttgc
     7981 tatcttctac ttttacagac gaatgtgggc ttccccaagt gaccatttcc tcagcaggtt
     8041 tggtagtatc aacagtaacg gtcgccaatt tctctgtcac ctgctgatag cctttaggtg
     8101 cttgtttttc atacaagtcg taggtgcccg aggtcaggtc cttaaaatga acctctcctt
     8161 gtgtgtttga ttttgcttct actttttgac tattaccatt tgatgaacgc agctcaaagg
     8221 ccgcttctgg caatggcaca ttgtcagttg acattttctt aaagcgaagc tgagccactc
     8281 gttttagatt tgctactgta atagagggct tagtaaattc acttgtccca gaaccactac
     8341 ccttagaagg agacaaggca atttcgtttt ggaagtagac gttattaaca ggattaccat
     8401 aagttacagc agtttcatta ttaaaactgg ccaaattgga ttgaacgact agtggcttac
     8461 cagattgatc cgttttccct gttattttaa caacaaaggc tttattttga agtttatctc
     8521 caaagtcaat tcgcaagcgc ttattagaat tttgagtact atcttgtggt aactttgcct
     8581 caaggtcttt tcttaaattc aattttgtta gatcaacccc ataactagtg ggtagcgtat
     8641 agtcctcttt aacttcataa atttctactt tttccaattg ggcactactt gtagcggaat
     8701 cctctgactt acttgttgag ttattagatt taaccaacct tccaaatccc cataaattca
     8761 tggtcgcata aggaatattg gtacgatttg ggttgacata aacataccaa gtaaagtcgc
     8821 ctgtttgtgt attgtagctt tcaatacttc cacccacatt gctcaatccg ttagtaacat
     8881 aattgttgtc ttgcccagat gcatttccat aaaggacatt aactgtccca ccatatttga
     8941 tttcctgatc acctatggta ctcttaaaat ctgatatcgt ttgattttct aaaacaacct
     9001 tattctctag gaataagctc aattctgcag acaactggac tttatctaaa ccagcgatat
     9061 aatttgtaaa agtataggtg agctgatgag tcttggcatc gtattttcca atcgcaagcg
     9121 gactattttc actgtcataa atgattttag ggatatcttg actgatacct ttaggattga
     9181 gacgtttgtc cagctgtaac acaaaggtat cgcctgggtt gattgtttca gatttatcct
     9241 tttgtttcaa ctcaaaagac attttaaaat aggcggcatg attgcggtag aaatccgctg
     9301 cgccactagt tacttctgtc cctccgtatt tagagaatac agacattgtt gatttttcca
     9361 actgtaaaga actactaaca tcttttgacc ctgctttact gatgatttcc ccattatagg
     9421 gattttcaac caacttggta tagccgttct cataaacagt cactgtccaa gtccgactgg
     9481 ttttatcata accatctggt gctttatctt cttttaaggt ataagtccct ggttggagat
     9541 tttgagcatc tataataccc tcttcgcttg aggtaaaggt ctgaacaggt tttcccttcc
     9601 catcctttgg tgtcagtgaa aaagttgctc ccgataaggg cttattgttt tggtcgactt
     9661 tcttgatttc aaaagatcct tgtttgtttg ctccatttct tgtttccgca tgtcctactg
     9721 atactccgga caatcctata aaaaccaata ataaacctaa aataaatcct gttaaggata
     9781 acaggaagct taacttatag ctattttttt gtgtcattta ttttctctct ccacattcat
     9841 aagcgacagc ctatgtccaa aactaaagac tacaggacaa ctgtcaaata gttattttac
     9901 cattttcagt tgtcaagtct acatcttatg gtcagagtat ttattttgat ggttttttag
     9961 cgttttttat gtcattatct gtaactcttt ctttttcaag cgcttaacgt acaaatcctt
    10021 tatctttatc tgcttagcta tttatagtat aattaaggta ggagatgatg gagagagagt
    10081 ttactattag gaaaaccttt tagtgtgcct accaaatagg aattttcatc attaacattt
    10141 actaaagctt ttacaagtct aaggaaaatg atacaactaa ggatgggggg caatctatca
    10201 aatggttata ttcgatttaa aatatgtgca aacattacac agcttgtctc aattacctat
    10261 ttcagtgatg tcacaagata aggcacttat tcaagtatat ggtaatgacg actatttatt
    10321 atgttactat caatttttaa agcatctagc tattcctcaa gccgcacaag atgttatttt
    10381 ttatgagggt ttatttgaag agtcctttat gatttttcct ctttgtcact acattattgc
    10441 cattggacct ttctaccctt attcacttaa taaagactat caggaacaat tagctaataa
    10501 ttttttaaaa cattcttctc atcgtagcaa agaagagctc ttgtcctata tggcacttgt
    10561 cccacatttt ccaattaata atgtgcggaa ccttttgata gctattgacg ctttttttga
    10621 cacacaattt gagacgactt gccaacaaac aattcatcaa ttgttgcagc attcaaaaca
    10681 gatgactgct gatcctgata tcattcatcg ccttaagcat attagcaaag catctagcca
    10741 a
//
LOCUS       DAUVUY010000065        10599 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.065, whole genome shotgun sequence.
ACCESSION   DAUVUY010000065 DAUVUY010000000
VERSION     DAUVUY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10599
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.065"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..582
                     /locus_tag="VOX58_001415"
     CDS             <1..582
                     /locus_tag="VOX58_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HES5878640.1"
                     /translation="VSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEISKIETGVNK
                     VEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASAIDPAKTNLS
                     VAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDIDEIIAEVNIS
                     DVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            636..1991
                     /locus_tag="VOX58_001416"
     CDS             636..1991
                     /locus_tag="VOX58_001416"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HES5878641.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            2115..2756
                     /locus_tag="VOX58_001417"
     CDS             2115..2756
                     /locus_tag="VOX58_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878642.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTPKQDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLAD
                     MQENLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSRP"
     gene            2819..3949
                     /locus_tag="VOX58_001418"
     CDS             2819..3949
                     /locus_tag="VOX58_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HES5878643.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FDSYGIRDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKTAGFHNISIDLIY
                     ALPGQTLDQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGVSFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            3959..4711
                     /locus_tag="VOX58_001419"
     CDS             3959..4711
                     /locus_tag="VOX58_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HES5878644.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGG
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKPHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCEFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQITSDGQLNAQAMIEWRAIKQTESETD"
     gene            4711..5475
                     /locus_tag="VOX58_001420"
     CDS             4711..5475
                     /locus_tag="VOX58_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HES5878645.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQERGIPYLLVTNN
                     TTRTPEMVQSMLANQFHVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            5475..6107
                     /locus_tag="VOX58_001421"
     CDS             5475..6107
                     /locus_tag="VOX58_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HES5878646.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
     gene            6552..>10599
                     /gene="cas9"
                     /locus_tag="VOX58_001422"
     CDS             6552..>10599
                     /gene="cas9"
                     /locus_tag="VOX58_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HES5878647.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKV
                     LTRSDKNRGKSNNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVRKDLIVKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATFIH"
BASE COUNT         3476 a         1744 c         2207 g         3172 t
ORIGIN      
        1 gtatctccag ataaaatatc agtcacaatt ggcaaaaaag aatctaaggt atttcctgtt
       61 agaggaagcg ttgatgctaa acaaattgca aatggttatg aaattagcaa aattgaaacg
      121 ggggttaata aggttgaagt gaccagtgat gaatctacca ttgctttaat tgatcacgtt
      181 gtggcaaagt tacctgatga tcaagtgtta gataggaatt atagtagtcg agtgacctta
      241 caagctgtct cagctgatgg aactatttta gcaagtgcaa ttgatcctgc aaaaacaaat
      301 ttatcagttg ctgtaaaaaa aataacgaaa tcagtcccga ttagagttga ggcagtgggg
      361 atgatggatg atagcttatc agatattcaa tacaaattgt caaaacaaac agctgttatc
      421 tcaggtagtc gagaggtctt agaagatatt gatgaaatta ttgcagaagt taacatttct
      481 gatgttacta aaaatactag taagaccgtg agtttgtctt ctagtcaggt gtcaattgag
      541 ccgtcagtag tgaccgttca gttgacaacc accaaaaaat aatggataat aactaggaaa
      601 attgaatgga ggaagccatt aggcttatat aaattatggg aaaatatttt ggaacagatg
      661 gtgttcgtgg cgaagcaaat gttgagctga cacctgagtt agcttttaaa ctgggccgat
      721 tcggtggtta tgttttaagt caacatgaga ccgaaagacc taaagttttt gtggcgcgtg
      781 acacccgtat ttcaggagaa atgttggagt cagccttgat tgctggtcta ctatctgttg
      841 gtattgaagt ttataaactt ggcgttttag cgacacctgg tgtctcttat ttagttagaa
      901 cagaaaaagc aagtgcaggt gtgatgattt ctgcaagtca taaccctgcg cttgacaatg
      961 gcatcaaatt ctttgggaat gatgggttta agttagctga tgaccaagaa ttggaaattg
     1021 aagctctctt agatgctccg gaggacacgc ttcctcgccc gagtgcagaa ggacttggaa
     1081 ccttggtaga ttatccagaa ggacttcgta aatatgaaaa gttcttggta acaactggta
     1141 ctgacttaag cggtatgaca gttgccttag acactgctaa tggagctgct tcagtgtcag
     1201 cacgtgatgt ctttttagat ttaaatgcag aaattgctgt cattggtgaa aaaccaaatg
     1261 gtcttaatat taatgacggt gttggttcaa cgcatccaga acaacttcaa gagttagtta
     1321 aggaaacagg agcagatctt ggtttagcct ttgatggcga tagcgaccgt ctgattgccg
     1381 tggacgaaac tggcgagatt gttgacggtg atcgtattat gtttatcatt ggtaaatact
     1441 tgtcagaaaa agggcttctt gctcacaata ccattgtcac aacggtgatg tctaacctcg
     1501 gttttcataa agctttagat aaacaaggta ttaataaagc aattacagca gttggagacc
     1561 gctatgtggt tgaagaaatg cgtagctcag gatacaatct tggtggtgaa caatcaggcc
     1621 atgtcattat catggactac aataccactg gagatggtca gttgacagct atccaattag
     1681 ccaaggtgat gaaggaaact gggaaatcac tttcggaatt agctgctgaa gtaaccattt
     1741 accctcaaaa actggtgaat attcgtgtgg aaaacagcat gaaagaccgt gctatggaag
     1801 ttccagctat tgcgaacatc attgcaaaaa tggaggatga aatggctggt aatggtcgta
     1861 ttctcgtgag accaagtggt acagaacctc ttcttcgtgt catggcagag gcgccaacag
     1921 atgccgaagt tgattattat gtcgatacta ttgctgatgt cgtccgtaca gaaatcggat
     1981 gcgataacta acgagttgag tacaaacaag aaaccttatt tctaaggctt ctgtcaacaa
     2041 ttggaaagag gcttggggcg tttagcccaa gtctcttttt ataaattgct aaagtagaag
     2101 agaaaggata tcggatgaca aaacagcaaa tcgaaaaact agcggactta ctgcctcctt
     2161 ttgcagccac actttactta gatatgattc ctgaatttca ggaaggttat gcttcatatg
     2221 tttatcaaac accaaaacaa gatattcaaa agcgccgcat caagcagtta actaaaaatt
     2281 ttgaacgatt actagatacc ccatcttttt atgtgatgcc tgtgacaaga aaaacagcag
     2341 agcaattgct agcagataaa ggcctagctt atcatttgtt atcgacagtt gcagctaatc
     2401 aagtgtttgc ggctgctgtt aacgaggatc agacgctttt tcaagtgctt cgaaatggag
     2461 caagagtagc ctttgtgatt cttcaagaaa agggggaatt tctagaaatg actttaggga
     2521 ttcttgaaac agtgacagaa aacgaggttc cagcggctat tgtggaggct gtccagacat
     2581 atcttctggc agatatgcaa gagaacctcc cttctttaag cattatggtc agtcaagaat
     2641 caagcaagct tttttaccaa gaacaaggat ttgttttgct agctaagggt tgcaagccag
     2701 gaaacgaaaa tgattctgac gtttgtcagc tcgtaaaata tttttccagg ccatgaagaa
     2761 tcaagggaaa tggcttattc atagaaacat tttatcatca aagtggtata ataagaccat
     2821 gtcaaaaaaa cctacttcag cctatgtgca cattcctttt tgcacacaaa tttgttatta
     2881 ttgcgatttt tcaaaagtct ttatccaaaa tcaacctgtt gatgcttatt tgaaagcctt
     2941 gattcaagaa tttgactctt atggcattcg agacctaaaa accctttata ttggaggagg
     3001 tacgcctaca gctatcactg ctaaacagtt ggagtatctc ctaaatcacc ttgaacgaaa
     3061 cctcaattta gatgatcttg aagaattcac aattgaggca aatcctggtg atttgacgcc
     3121 agaaaaaatt gcggtgcttc agaggtcagc tgtcaatcgg atttccttgg gggtgcagac
     3181 ttttaataac aagcaattaa agcagattgg ccgtagccat aatgaagaac aaatttatag
     3241 caccattgcc aatttaaaaa ctgcaggctt tcacaatatt tccattgatt taatttatgc
     3301 cttgccaggt caaaccttag atcaagtcaa ggaaaatgtt gctaaagctc tggcacttga
     3361 tattcctcac cttagtcttt atagccttat tttggaacat cacactgttt ttatgaacaa
     3421 aatgcgacgt ggtaaactta acttaccaac ggaagacttg gaagcggaaa tgtttgagta
     3481 cattatttca gaaatggaag ctaatggttt tgagcattat gaaatttcca attttaccaa
     3541 accaggtttt gaaagtcgcc ataacttgat gtattgggat aatgtggagt attttggatg
     3601 tggtgcaggg gcttcaggtt accttaatgg cattcgttat cagaatcggg tgcccatcca
     3661 gcattatctt aaagcggttg aagcagggaa tgctagacta aatgaagaag tgctgcgcaa
     3721 agaggaaatg atggaagagg aactcttttt gggcttacgt aaaaagacag gagtctccat
     3781 ccagcggttc caagaaaaat ttggtgtttc ttttgaggag cgttatggaa atattgttag
     3841 ggagcttcaa aaccaaggac tgcttgtcaa agatgacgct tttgtcagaa tgactaaaaa
     3901 gggtcttttc ttaggtgatt ctgttgctga gcggtttata ttagattagg agagtattat
     3961 gggattaagt tatcaggagg agttgacact tccttttgaa ttatgtgatg tcaaatcaga
     4021 tataaaattg ccccttttat tagactattg tttgatggtt tctggtagac agtctgcgca
     4081 attaggacga agcaacaaca accttttagt cgattacaag cttgtttgga ttgtaacgga
     4141 ttatgagatc actattcatc gcttgccaca ttttcaagaa accatcacca ttgaaacaaa
     4201 agccctctcc tacaataaat ttttttgtta tcgccaattt tatatttatg atcaagaggg
     4261 gggtctttta gtggatatct tagcctattt tgctttgtta aacccagata cgcgaaaagt
     4321 ggcaactatt ccagaagatt tagtagcgcc ttttaagact gattttgtta aaaagccaca
     4381 ccgtgttcct aaaatgcctc ttttagaaca atcaattgat cgtgattatt atgtgcgtta
     4441 ttttgatatt gatatgaatg gtcatgtcaa caacagtaaa tatttagatt ggatgtatga
     4501 tgtgttgggg tgtgagtttt taaaaacgca tcagcctctt aagatgactt tgaaatatgt
     4561 taaagaagtc tcaccaggcg gtcaaattac ttccagttac catttggatc aattaacttc
     4621 ttaccatcaa atcacctcag atgggcagct gaatgcccaa gccatgattg aatggcgagc
     4681 gattaaacaa acagaaagcg agacagacta gtgccttata agggttattt aattgattta
     4741 gacggaacga tttaccaagg gaaaaaccgt attccagcag gggagcggtt tatcaaacgc
     4801 ctacaagaaa gaggcattcc ctatttatta gtgactaata ataccacaag aacacccgaa
     4861 atggtgcaaa gcatgctggc taatcagttt catgttgaga caggcattga gaccatttat
     4921 acggctacca tggcaaccgt cgattacatg aatgacatga accgtggcaa gacagcttac
     4981 gttattggag aaacagggct caaatcagct attgcagcag caggttacgt agaagaactt
     5041 gagaatcccg cttacgtggt ggttggttta gacagtcagg tgacctatga gatgttggct
     5101 atagccacct tggctattca aaaaggtgcc ctctttattg ggacaaatcc tgatttaaac
     5161 attccaaccg aacgtggttt gatgccagga gcaggtgcat tgaatgcctt actagaagct
     5221 gccacacgtg taaaacctgt ttttattggc aagcccaacg ctatcattat gaataaatca
     5281 ctagaggtat tgggcattca gcgctctgag gctgtcatgg ttggcgataa ttacttgact
     5341 gatatcatgg caggtattca aaatgatatt gcaacgatcc tcgtgacaac aggctttacc
     5401 agacctgaag aggtaccaac cttaccaatt cagccagatc atgtcctatc tagccttgat
     5461 gagtggaggt tgtgatggta gaaaacacta aattactatg tagctgggta tggttattag
     5521 cgttagctat tctcataacg atctattcaa cgtggctgtg gtatcctctt gaggtggacc
     5581 atctaaagtt agaacaagtt gtttttatga gcaaggatgc tatcttgcat aactataacg
     5641 gactactaaa ttacctcacc aatccttttg tgacaaggtt ggaatttgct aattttcact
     5701 catcagcaga cggactcaag cattttgcag atgtgaagtg gctttttcac ctaactcaag
     5761 tcgtttttct tggcctcctt tacccaaccc ttaagacttt cacacaaagg ttaaaaacca
     5821 aacgtttctg gctccttcaa aaacctttaa tactagcagc tttgtttcct cttatgattg
     5881 gtctaatggc tagtttcatt gggtttgaac atttttttac tctttttcat caggtgcttt
     5941 ttgtaggaga tagcagctgg ctctttgatc ccttgaaaga tcctgtgatt tggatccttc
     6001 cagaagtctt tttcttgcac tgtttccttt tctttatgat agtttacgaa atcatcctgt
     6061 ggagcttagt aggtttagca agatggcagc gcctaaatgt agaatgataa aacaagcgtt
     6121 ttgaaagcgc ttgtttttta gtttgcagtc agagtagaat agaagtatca aaaaaaagca
     6181 ccgactcggt gccacttttt caagttgata acggactagc cttattttaa cttgctatgc
     6241 tgttttgaat ggttccaaca agattatttt ataactttta taacaaataa tcaaggagaa
     6301 attcaaagaa atttatcagc cataaaacaa tacttaatac tatagaatga taacaaaata
     6361 aactactttt taaaagaatt ttgtgttata atctatttat tattaagtat tgggtaatat
     6421 tttttgaaga gatattttga aaaagaaaaa ttaaagcata ttaaaccaat ttcggaggtc
     6481 attaaaacta ttagtgaaat catcaaactc attatggatt taatttaaac tttttatttt
     6541 aggaggcaaa aatggataag aaatactcaa taggcttaga tatcggcaca aatagcgtcg
     6601 gatgggcggt gatcactgat gattataagg ttccgtctaa aaagttcaag gttctgggaa
     6661 atacagaccg ccacagtatc aaaaaaaatc ttataggggc tcttttattt gacagtggag
     6721 agacagcgga agcgactcgt ctcaaacgga cagctcgtag aaggtataca cgtcggaaga
     6781 atcgtatttg ttatctacag gagatttttt caaatgagat ggcgaaagta gatgatagtt
     6841 tctttcatcg acttgaagag tcttttttgg tggaagaaga caagaagcat gaacgtcatc
     6901 ctatttttgg aaatatagta gatgaagttg cttatcatga gaaatatcca actatctatc
     6961 atctgcgaaa aaaattggta gattctactg ataaagcgga tttgcgctta atctatttgg
     7021 ccttagcgca tatgattaag tttcgtggtc attttttgat tgagggagat ttaaatcctg
     7081 ataatagtga tgtggacaaa ctatttatcc agttggtaca aacctacaat caattatttg
     7141 aagaaaaccc tattaacgca agtggagtag atgctaaagc gattctttct gcacgattga
     7201 gtaaatcaag acgattagaa aatctcattg ctcagctccc cggtgagaag aaaaatggct
     7261 tatttgggaa tctcattgct ttgtcattgg gtttgacccc taattttaaa tcaaattttg
     7321 atttggcaga agatgctaaa ttacagcttt caaaagatac ttacgatgat gatttagata
     7381 atttattggc gcaaattgga gatcaatatg ctgatttgtt tttggcagct aagaatttat
     7441 cagatgctat tttactttca gatatcctaa gattaaatag tgaaataact aaggctcccc
     7501 tatcagcttc aatgattaaa cgctacgatg aacatcatca agacttgact cttttaaaag
     7561 ctttagttcg acaacaactt ccagaaaagt ataaagaaat cttttttgat caatcaaaaa
     7621 acggatatgc aggttatatt gatgggggag ctagccaaga agaattttat aaatttatca
     7681 aaccaatttt agaaaaaatg gatggtactg aggaattatt ggcgaaacta aatcgtgaag
     7741 atttgctgcg caagcaacgg acctttgaca acggctctat tccccatcaa attcacttgg
     7801 gtgagctgca tgctattctg agaagacaag aagactttta tccattttta aaagacaatc
     7861 gtgagaagat tgaaaaaatc ttgacttttc gaattcctta ttatgttggt ccattggcgc
     7921 gtggcaatag tcgttttgca tggatgactc ggaagtctga agaaacaatt accccatgga
     7981 attttgaaga agttgtcgat aaaggtgctt cagctcaatc atttattgaa cgcatgacaa
     8041 actttgataa aaatcttcca aatgaaaaag tactaccaaa acatagtttg ctttatgagt
     8101 attttacggt ttataacgaa ttgacaaagg tcaaatatgt tactgaggga atgcgaaaac
     8161 cagcatttct ttcaggtgaa cagaagaaag ccattgttga tttactcttc aaaacaaatc
     8221 gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa tgttttgata
     8281 gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc attaggtacc taccatgatt
     8341 tgctaaaaat tattaaagat aaagattttt tggataatga agaaaatgaa gatatcttag
     8401 aggatattgt tttaacattg accttatttg aagataggga gatgattgag gaaagactta
     8461 aaacatatgc tcacctcttt gatgataagg tgatgaaaca gcttaaacgt cgccgttata
     8521 ctggttgggg acgtttgtct cgaaaattga ttaatggtat tagggataag caatctggca
     8581 aaacaatatt agattttttg aaatcagatg gttttgccaa tcgcaatttt atgcagctga
     8641 tccatgatga tagtttgaca tttaaagaag acattcaaaa agcacaagtg tctggacaag
     8701 gcgatagttt acatgaacat attgcaaatt tagctggtag ccctgctatt aaaaaaggta
     8761 ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg cataagccag
     8821 aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt
     8881 cgcgtgagcg tatgaaacgt attgaagaag gtatcaaaga attaggaagt cagattctta
     8941 aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa gctctatctc tattatctcc
     9001 aaaatggaag agacatgtat gtggaccaag aattagatat taatcgttta agtgattatg
     9061 atgtcgatca cattgttcca caaagtttca ttaaagacga ttcaatagac aataaggtct
     9121 taacgcgttc tgataaaaat cgtggtaaat cgaataacgt tccaagtgaa gaagtagtca
     9181 aaaagatgaa aaactattgg agacaacttc taaacgccaa gttaatcact caacgtaagt
     9241 ttgataattt aacgaaagct gaacgtggag gtttgagtga acttgataaa gctggtttta
     9301 tcaaacgcca attggttgaa actcgccaaa tcactaagca tgtggcacaa attttggata
     9361 gtcgcatgaa tactaaatac gatgaaaatg ataaacttat tcgagaggtt aaagtgatta
     9421 ccttaaaatc taaattagtt tctgacttcc gaaaagattt ccaattctat aaagtacgtg
     9481 agattaacaa ttaccatcat gcccatgatg cgtatcttaa tgccgtcgtt ggaactgctt
     9541 tgattaagaa atatccaaaa cttgaatcgg agtttgtcta tggtgattat aaagtttatg
     9601 atgttcgtaa aatgattgct aagtctgagc aggaaatagg caaagcaacc gcaaaatatt
     9661 tcttttactc taatatcatg aacttcttca aaacagaaat tacacttgca aatggagaga
     9721 ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg agaaattgtc tgggataaag
     9781 ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga
     9841 aaacagaagt acagacaggc ggattctcca aggagtcaat tttaccaaaa agaaattcgg
     9901 acaagcttat tgctcgtaaa aaagactggg atccaaaaaa atatggtggt tttgatagtc
     9961 caacggtagc ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt
    10021 taaaatccgt taaagagtta ctagggatca caattatgga aagaagttcc tttgaaaaaa
    10081 atccgattga ctttttagaa gctaaaggat ataaggaagt tagaaaagac ttaatcgtta
    10141 aactacctaa atatagtctt tttgagttag aaaacggtcg taaacggatg ctggctagtg
    10201 ccggagaatt acaaaaagga aatgagctgg ctctgccaag caaatatgtg aattttttat
    10261 atttagctag tcattatgaa aagttgaagg gtagtccaga agataacgaa caaaaacaat
    10321 tgtttgtgga gcagcataag cattatttag atgagattat tgagcaaatc agtgaatttt
    10381 ctaagcgtgt tattttagca gatgccaatt tagataaagt tcttagtgca tataacaaac
    10441 atagagacaa accaatacgt gaacaagcag aaaatattat tcatttattt acgttgacga
    10501 atcttggagc tcccgctgct tttaaatatt ttgatacaac aattgatcgt aaacgatata
    10561 cgtctacaaa agaagtttta gatgccactt ttatccatc
//
LOCUS       DAUVUY010000066        10112 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.066, whole genome shotgun sequence.
ACCESSION   DAUVUY010000066 DAUVUY010000000
VERSION     DAUVUY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.066"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            90..1010
                     /gene="lmb"
                     /locus_tag="VOX58_001423"
     CDS             90..1010
                     /gene="lmb"
                     /locus_tag="VOX58_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HES5878648.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            1023..3500
                     /locus_tag="VOX58_001424"
     CDS             1023..3500
                     /locus_tag="VOX58_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HES5878649.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGSATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPAPGRRKAPIPDVTP
                     NPGQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEED
                     GLIFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSDH
                     SKPSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHF
                     HYIGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGK
                     VGYMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSS
                     LPMHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPG
                     HEESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKE
                     TFVWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQ
                     ADKSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIF
                     QPEGVQFYNKNGELVTYDIKTLQQINP"
     gene            3669..3869
                     /locus_tag="VOX58_001425"
     CDS             3669..3869
                     /locus_tag="VOX58_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HES5878650.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(3951..4577)
                     /locus_tag="VOX58_001426"
     CDS             complement(3951..4577)
                     /locus_tag="VOX58_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878651.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIIISHEFDMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(4561..5364)
                     /locus_tag="VOX58_001427"
     CDS             complement(4561..5364)
                     /locus_tag="VOX58_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878652.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGNLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(5376..6197)
                     /locus_tag="VOX58_001428"
     CDS             complement(5376..6197)
                     /locus_tag="VOX58_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878653.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNASLRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVSLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVVFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(6194..7171)
                     /locus_tag="VOX58_001429"
     CDS             complement(6194..7171)
                     /locus_tag="VOX58_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878654.1"
                     /translation="MKRTTIIIIWKIIRCVTLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRVKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(7284..8912)
                     /locus_tag="VOX58_001430"
     CDS             complement(7284..8912)
                     /locus_tag="VOX58_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HES5878655.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQINIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELADKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINIGKQGVHSHGHDWSLLTNIAEWTWDESTK"
     gene            complement(9642..>10112)
                     /locus_tag="VOX58_001431"
     CDS             complement(9642..>10112)
                     /locus_tag="VOX58_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HES5878656.1"
                     /translation="EYAANDFKAGDRIAVFSVPFDWNYLSKGKVTAYTYGGITPYQKT
                     SIPKNIPVNLWINRKQISVPYNEISTNKTTVTAQEIDLKVRKFLISQHQLYSSGSSYK
                     SGKLVFHTNDNSDKYSLDLFYTGYRDKESIFKVYKDNKSFNIDKIGHLDIEIDS"
BASE COUNT         3337 a         1903 c         1971 g         2901 t
ORIGIN      
        1 aaaagatggt agttgccatt gacaaagcat tgataaagga gtaaaattaa ctggttaata
       61 actggttaaa ttataattga ggaggtacta tgaaaaaagg tttttttctc atggctatgg
      121 tcgtgagttt agtaatgata gcagggtgtg ataagtcagc aaaccccaaa cagcctacgc
      181 aaggcatgtc agttgtaacc agcttttacc caatgtatgc gatgacaaaa gaagtatctg
      241 gagacctcaa tgatgtgagg atgatccaat caggtgcagg cattcattcc tttgaaccgt
      301 ctgtaaatga tgtggcagct atttatgacg cggatttgtt tgtttaccat tcacatacct
      361 tagaagcttg ggcaagggat ctagacccta atttaaaaaa atcaaaggtt gatgtgtttg
      421 aagcgtcaaa acctttgaca ctagatagag tcaaagggct agaagatatg gaagtcacac
      481 aaggcattga ccctgcgaca ctttatgacc cacatacctg gacggatccc gttttagctg
      541 gtgaggaagc tgttaatatc gctaaagagc taggacgttt ggatcctaaa cacaaagaca
      601 gttacactaa aaaggctaag gctttcaaaa aagaagcaga gcaactaact gaagaataca
      661 ctcaaaaatt taaaaaggtg cgctcaaaaa cattcgtgac gcagcacacg gcattttctt
      721 atctggctaa acgattcggc ttgaaacaac ttggtatctc gggtatttct ccagagcaag
      781 agccctctcc tcgccaattg aaagaaattc aagactttgt caaagaatac aacgtcaaga
      841 ctatttttgc agaagacaat gtcaatccca aaattgctca tgctattgcg aaatcaacag
      901 gagctaaagt aaagacatta agtccacttg aagctgctcc aagcggaaac aagacatatc
      961 tagaaaatct tagagcaaat ttggaagtgc tctatcaaca gttgaagtaa aggagattat
     1021 tagtgaagaa aacatatggt tatatcggct cagttgctgc cattttacta gctactcata
     1081 ttggaagtta ccaacttggt aagcatcata tgggttcagc aacaaaggac aatcaaattg
     1141 cctatattga tgatagcaaa ggtaaggcaa aagcccctaa aacaaacaaa acgatggatc
     1201 aaatcagtgc tgaagaaggc atctctgctg aacagatcgt ggtcaaaatt actgaccaag
     1261 gctatgtgac ctcacacggt gaccattatc atttttacaa tgggaaagtt ccttatgatg
     1321 cgattattag tgaagagttg ttgatgacgg atcctaatta ccattttaaa caatcagacg
     1381 ttatcaatga aatcttagac ggttacgtta ttaaagtcaa tggcaactat tatgtttacc
     1441 tcaagccagg tagtaagcgc aaaaacattc gaaccaaaca acaaattgct gagcaagtag
     1501 ccaaaggaac taaagaagct aaagaaaaag gtttagctca agtggcccat ctcagtaaag
     1561 aagaagttgc ggcagtcaat gaagcaaaaa gacaaggacg ctatactaca gacgatggct
     1621 atatttttag tccgacagat atcattgatg atttaggaga tgcttattta gtacctcatg
     1681 gtaatcacta tcattatatt cctaaaaagg atttgtctcc aagtgagcta gctgctgcac
     1741 aagcctactg gagtcaaaaa caaggtcgag gtgctagacc gtctgattac cgcccgacac
     1801 cagccccagc cccaggtcgt aggaaagccc caattcctga tgtgacgcct aaccctggac
     1861 aaggtcatca gccagataac ggtggctatc atccagcgcc tcctaggcca aatgatgcgt
     1921 cacaaaacaa acaccaaaga gatgagttta aaggaaaaac ctttaaggaa cttttagatc
     1981 aactacaccg tcttgatttg aaataccgtc atgtggaaga agatgggttg atttttgaac
     2041 cgactcaagt gatcaaatca aacgcttttg ggtatgtggt gcctcatgga gatcattatc
     2101 atattatccc aagaagtcag ttatcacctc ttgaaatgga attagcagat cggtacttag
     2161 ccggccaaac tgaggacaat gactcaggtt cagatcactc aaaaccatca gataaagaag
     2221 tgacacatac ctttcttggt catcgcatca aagcttacgg aaaaggctta gatggtaaac
     2281 catatgatac gagtgatgct tatgttttta gtaaagaatc cattcattca gtggataaat
     2341 caggagttac agctaaacac ggagatcatt tccactatat aggatttgga gaacttgaac
     2401 aatatgagtt ggatgaggtc gctaactggg tgaaagcaaa aggtcaagct gatgagcttg
     2461 ctgctgcttt ggatcaggaa caaggcaaag aaaaaccact ctttgacact aaaaaagtga
     2521 gtcgcaaagt aacaaaagat ggtaaagtgg gctatatgat gccaaaagat ggcaaggact
     2581 atttctatgc tcgtgatcaa cttgatttga ctcagattgc ctttgccgaa caagaactaa
     2641 tgcttaaaga taagaagcat taccgttatg acattgttga cacaggtatt gagccacgac
     2701 ttgctgtaga tgtgtcaagt ctgccgatgc atgctggtaa tgctacttac gatactggaa
     2761 gttcgtttgt tatccctcat attgatcata tccatgtcgt tccgtattca tggttgacgc
     2821 gcgatcagat tgcaacaatc aagtatgtga tgcaacaccc cgaagttcgt ccggatgtat
     2881 ggtctaagcc agggcatgaa gagtcaggtt cggtcattcc aaatgttacg cctcttgata
     2941 aacgtgctgg tatgccaaac tggcaaatta tccattctgc tgaagaagtt caaaaagccc
     3001 tagcagaagg tcgttttgca acaccagacg gctatatttt cgatccacga gatgttttgg
     3061 ccaaagaaac ttttgtatgg aaagatggct cctttagcat cccaagagca gatggcagtt
     3121 cattgagaac cattaataaa tctgatctat cccaagctga gtggcaacaa gctcaagagt
     3181 tattggcaaa gaaaaatgct ggtgatgcta ctgatacgga taaacccaaa gaaaagcaac
     3241 aggcagataa gagcaatgaa aaccaacagc caagtgaagc cagtaaagaa gaagaaaaag
     3301 aatcagatga ctttatagac agtttaccag actatggtct agatagagca accctagaag
     3361 atcatatcaa tcaattagca caaaaagcta atattgatcc taagtatctc attttccaac
     3421 cagaaggtgt ccaattttat aataaaaatg gtgaattggt aacttatgat atcaagacac
     3481 ttcaacaaat aaacccttaa ccaaaagaag atctcattgt taaagcactg ctttgtcaaa
     3541 gcaagttacc gtgattttga agtcatttta tgtaacgagt agtgataaaa gttggataat
     3601 agcggttttc ttttgcaaag aaatggtatc catgttagaa taataaaaaa aagaggagga
     3661 ttctaatcat gtcagaagaa aaattaaaat caaaaattga gcaagcatct ggtggcctaa
     3721 aagaaggtgc tggtaagttg acaggtgata aagagctaga agcaaaagga tttgttgaaa
     3781 aaacaattgc taaaggcaaa gaacttgcag acgatgcaaa agaagcggtt gaaggtgctg
     3841 tagatgccgt taaggaaaaa ttaaaataat taaaaaaacc agctgtaagc tggttttttt
     3901 aatggtgtga ttttgttagg aaaagactca ataggtaata accgtaggtg ttaggtatgc
     3961 ttgaacagtt gagtacgatt ctcttcaatc atatagcacg cgtcacaaag tttttctaac
     4021 atatcaaact catgactaat aatgatgaga cctaaatttc tatcctttac aatctccaac
     4081 aggcttttcc atacactagc ttgtgtaatg ctatccaaca tagtagtcat ttcatctgca
     4141 ataaggtatt tcgtctctgg atgtaatgaa cgcacaatcg aaaagcgttg caattcccct
     4201 cccgagagtt cactagggcg acgctttagc catttttctt gaatgccaaa agcatctcgc
     4261 aaatcttgac ttggatagta ggcttcttcc aaactttttt tcataggcca taaaggattc
     4321 atggtttgtt caggatgttg ttggataagt tgtacaggtc taaatgcttt gttaggcaaa
     4381 tgactgccat caactaatac ttcaccagat ttaggctgca aaaaaccggc aagcactctg
     4441 gataaacttg tttttccaca cccactttgt ccaaatatcc ctaagatttg accaggtgct
     4501 acctctaaat caatctcctt aaaaagccat tgatcttttt tatgataaaa gccaagcttt
     4561 ttagcctcta aggtcatgag taactccttt caaaaagtct tgctgaggga gtgtgcgcca
     4621 taaacgtctc gcaaattctg tttgtaattg ctccccacct ccgctaaaaa agctagctgg
     4681 ggctgtttca atagcttttc cctctttaaa aatagttata cgatccgcaa tttgactagc
     4741 tgctacaata tcatgagtga taaatatgac gcttattcct ttatctgcaa aggagcgtag
     4801 ttgatctaaa accatttgca gagcatctgg atgtaatcca ggggtgggct catccgcaat
     4861 aatcaaagaa accgtatcac taatacacgt tgtaaacaaa acacgtcgga gcattccgcc
     4921 agaaagttgg aaaggataca agttaccatc actttctttt aaaccaaact gttgaaacaa
     4981 tccttcttga gtagccttag cattttctga gatacccaag cgcacctgat gcttgacttt
     5041 catagatgga tctaaataat taacggattg tggaatcaac gtcatttctt ttcctcgcaa
     5101 ctgtttgatg cgtttagaag ttagtgattg accacgataa atcatatctc ctgttacagc
     5161 tgcattttta ggaagaatat ccataatagc atgtgctaat aaactcttgc ctgaaccact
     5221 agcacctata atagctaata actcaccttt tttaacttct aaattcagcg cttggatcgg
     5281 tgtggattga aatggtttta aaaatcttcc gtattgagtg aaggtgatgg agaggtcttt
     5341 aatgcttaat aatgtttctg tcatatctct acctcctaaa aatgatccgt ttgagggtaa
     5401 aagagtttct ttaaagattc tccgatagta tcaaaggcat tgacaactaa aataagataa
     5461 aggcctggaa aaaccaccaa ccaccaattg ccaagagaga tatgcttagc tgcttctgac
     5521 agaatgatac caaccgaagg ttgttcggca gaaaggccaa atcctaagaa agtcatggat
     5581 gcttcatgca agatgacgtg cggaaataag aggataaacc caatgaaaat ttgagaagca
     5641 atcaaaggca ggatatgatg cctcacaata taataaggcg tttttcccat gcttttagag
     5701 agctggacaa aggctttatt ctttagatca tagacttcat tgcggataag ccttgctaga
     5761 gagggccaat gggtaacagc cgttgcaatg ataacccctt gagccccttt accaacaaca
     5821 aaagaaatga gaatcataaa aatcaaatga ggcataccaa taaacaaatc aaccaaccag
     5881 gctattattt tatcaataag gctatttcct agacctgcaa gcactccaaa aacggtcgca
     5941 agagagactc ccataagggc acctaataag ccgacttgta aagagaaata aagcccttta
     6001 atcgttctga cgaacatatc cctacctaaa ccatctgtcc caaaaagatg gtttaaggaa
     6061 ggagcgaggt tgcgtaaaga tgcattggtt tccaaaggcg tcctatagaa ataaaggttt
     6121 aaggctagaa tactaagaat gagagaaatg gcgataccta gttgccataa aaccatcgtt
     6181 cgacgtttca atatcatact tttctcctta actgtggatt gatgatgcta tttaaaatat
     6241 ccgcaataag attgcccgca aaaacaaata atgtccctat catcacgata gctagaagga
     6301 gcggtgtatc acttttaagt cctgcttcag tgagggtaga ccctaagcca ggatatgaga
     6361 aaacttgctc agcaagaaca gatcctccaa acaattctcc aaaataggaa aaatgcagtg
     6421 taatagctgg tacgatagca tttctaaggc aatgatgttt aaaaatttgc cactgtgttt
     6481 ccccacgcgc tctggcaaat aagacatatt cactagaaag caccgacatc attttagttc
     6541 tcgtatgaag ggtgacattg gcaatgccta gaatacttaa cgtgaaaaca ggtaacataa
     6601 ggtgcttaac ccgatcagct aacgtaatat cttgactcaa agtgcctatc ggggaagaaa
     6661 taccaatcgg gaaccacccc agctggacag aaaagattaa taaaaaaatg aggccaatcc
     6721 aaaacgttgg tactgatatc tgaaggtaag aaaaccacct gacaactcga tcaagtaatt
     6781 tcccttgatg gaaagcggat aacgttccta agataaatcc aataagaccc gataagatcc
     6841 aagagagtcc cataagtatg aaagaagcac ctgcccgtga tctaataata tcactaacag
     6901 gttgccgata aacgagcgag gtccctaaat gtccctgtat cacatttttc aaccaaataa
     6961 aatactggac tagagctggc ttatccaagc catagtggtg agcaatcgct ttgtactgag
     7021 caggggttag tgatgtgtca taattgacac ttgccatgac tggatctact ggagattgtt
     7081 ttaacaaaac gaaggtcaaa acagataccc caaaaataag cgtgacacat ctgatgattt
     7141 tccagatgat aataatggta gtacgtttca caatgaaatc tccttaatcg ataagaaaag
     7201 gagccgagga aacgatcagc acagttttgt gtatcttgtt ttctcagact ccaaacttaa
     7261 agtcgctgta ttaactaaac gcgttactta gttgattcat cccaagtcca ctcggcaatg
     7321 ttagtcaata atgaccaatc atgaccatga ctgtggacgc cctgtttacc tatattgata
     7381 cgtttatcac caatataagt atggttaagg ctcaccaacc atacatttgg caaatctcca
     7441 agagtagaag cacctgtttt gccatcccac tgcgctaact tccaatattc gttagctttg
     7501 tcaaggtcag aagatgtcat tgctttgtca aggtacttag tcacggtagg attgttataa
     7561 aacgtaatat tggtccaacc tttacctgct aggcttggat gatgtgattc ataaaattgc
     7621 tgcgcgtgat gacgtcctcc ggcataaagt aaggctgagt catgtgactt cgttgccatt
     7681 tcatcccagt tactagcttt gagtttaata gtaatcccta gggctttggc ttgctctgct
     7741 acttcaacgg ctaagttcgc tcgcaattga tcattagtag ggtagtacag atcaaacgct
     7801 gcatcaaggt cacctttttt acggctaccg tctgcttgtt ctttccatcc agcttttgtc
     7861 aacaattgct tagctttagc tactttatta tctttaatgg ctgtttttgg attccaaaat
     7921 ggtgttttat caataattga ataagctggt ttaccataac catttaaaac cgtatcgaga
     7981 actttttgcc tatttaaacc aatagtcaag gcttttcgga ttgctggatc actagtgaca
     8041 tcatttccta caggataacc atcaggagaa tcagtgatga cgcctttttt cacataaggt
     8101 aatgataagc cgcgcacatc atttgatgga atatcaagga ggcgggtgcc tttgactttt
     8161 ttatcagcaa gttctggcgt tgcgtagatc atatcaacat caccagattc taaagctgct
     8221 agtgctgtgt tttcatcaag taagacccaa gtccattttt taaagtatgg ttttttccca
     8281 tgccaataag ggttacgaac aaaaatagct tgttctccag ccttatattc ttttaccatg
     8341 taaggtcctg aaccgatagg attgctctta tacttatcat tgtaatgttt tttagggacg
     8401 attgggattt cagtcaactg tgctgtaaat gtcgaatgcg cctcagtcaa atggatattg
     8461 atctgatttt tcccaactac ttcaacgttc ttgatgaagg ttagatccca agcctttcca
     8521 tctgctttca acatatcata agtaaactta acatcatcag cagtaacagg ctcaccattt
     8581 gagaatttaa aatcatcatg caagtcaaac gaccaagtca gcccatcttc agagagatgg
     8641 tatgttttag caagctctcc ttttatatct agttcaggag aacgtttcaa tagagtgcta
     8701 tgagtgatat tcccttcatt gtggactcca taacggtcct ttggatcgaa ttcatgaggg
     8761 agctttgccc ccatagaaac gacaagttcg tcttttggac gttgtttgcg ctgacgttct
     8821 tttgtttgag gcttttgttg ttgacatgca actaaaataa gcccagtcaa aaataacgtg
     8881 ataatagaga agtattttag gtattttgac acaatcaaat ctccttattt ctaattaata
     8941 aataataata gtaacgactc catctcatta tagcaataaa caaataggag ggcaattgtt
     9001 taactggtta attagaattt ttgttatttt taggaagata ctcaggactt ttttataagt
     9061 tgatgtatta acaaaagata aagaggtttc atgacttatt ttagattaaa aaaagaatta
     9121 aactgatcac taagaaataa aggaattgta gtttacgaag ggaatcaaaa acttcagata
     9181 gccattaata caggtaaaaa taacagtgaa aaaagtcttt tttgatactt tttttgaggt
     9241 ggttcgggcg gcagcgactt tctccacagg aaaaaacatc tgttttggtc ttggcttact
     9301 tcgttcgcag aaataggaaa tcgggtatca cattagcaga gtagttcatg ttttttgagc
     9361 atcacactgg atctgtctac gttctaaaag ctttcttcat ttttgtatca taccccctac
     9421 aagtagaaaa agtataacaa agaaaattta taaacagaaa tggctagtat tatcaaggaa
     9481 tttatcaatt tttgtttttt aaaattaata tagaaagtat agcattctag aacaaattta
     9541 gaacaaaacc taagtgatat aaaaaaagac atacttactt tttagaggat tcaatctatt
     9601 tggaatagct tattgtataa aagttatatt gaaaaattga attaggagtc aatttctata
     9661 tctaaatgcc caattttatc tatattgaaa gatttattgt ctttgtatac tttaaaaata
     9721 ctttctttat ctctatatcc tgtatagaaa agatcgagag aatatttatc tgaattatca
     9781 tttgtatgaa aaactaattt accacttttg tagctagaac cagaagaata taattgatgt
     9841 tgtgatatta aaaattttct aacctttaga tcaatttctt gagctgtaac tgttgttttg
     9901 ttagttgata tttcgttgta aggaacagag atctgctttc tattaatcca taaattaaca
     9961 gggatatttt taggtattga agttttttgg tagggtgtta ttccgccgta cgtatatgct
    10021 gtgacttttc cttttgataa atagttccaa tcaaatggga cggagaacac agctattcta
    10081 tctccagctt taaaatcatt ggctgcatat tc
//
LOCUS       DAUVUY010000067         9757 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.067, whole genome shotgun sequence.
ACCESSION   DAUVUY010000067 DAUVUY010000000
VERSION     DAUVUY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.067"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            1..388
                     /gene="ipk"
                     /locus_tag="VOX58_001432"
                     /pseudo
     CDS             1..388
                     /gene="ipk"
                     /locus_tag="VOX58_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514767.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; frameshifted; internal stop; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
     gene            457..900
                     /locus_tag="VOX58_001433"
     CDS             457..900
                     /locus_tag="VOX58_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HES5878657.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            904..1623
                     /locus_tag="VOX58_001434"
     CDS             904..1623
                     /locus_tag="VOX58_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HES5878658.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            1616..2431
                     /locus_tag="VOX58_001435"
     CDS             1616..2431
                     /locus_tag="VOX58_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HES5878659.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVLGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(2471..2854)
                     /locus_tag="VOX58_001436"
     CDS             complement(2471..2854)
                     /locus_tag="VOX58_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HES5878660.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWESITINQENWDLTPFLKHL"
     gene            complement(2905..4161)
                     /locus_tag="VOX58_001437"
     CDS             complement(2905..4161)
                     /locus_tag="VOX58_001437"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HES5878661.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLFLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            4253..6565
                     /locus_tag="VOX58_001438"
     CDS             4253..6565
                     /locus_tag="VOX58_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HES5878662.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTIRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADSNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            6829..>9757
                     /locus_tag="VOX58_001439"
     CDS             6829..>9757
                     /locus_tag="VOX58_001439"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HES5878663.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEII
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAA"
BASE COUNT         2851 a         1807 c         2129 g         2970 t
ORIGIN      
        1 gtgaagtggt ttgtcgcatt ttggggctgt tatcgtcttg ggtggttttg gttaaaccag
       61 attttggtat ctcgacttga acattttttc tagatattaa ttgtaaaatc atttcaagag
      121 tgtctactac tcatttggtt gcggcgattg aagcgggtaa ctataatgat gggatattga
      181 ctgaaatgaa caatttgctg gaagatattt tcattgccaa gcgacccttt attcaaaaaa
      241 taaaagagaa aacactgcaa gctggtgctg ctactacttt gatgacagga agtggaccga
      301 ctgtttttgc tttgtgccag accgaaaaac aaactataat agtttaaaag gactttgcaa
      361 ggaagttttt aaggtgagaa tgttgtaata ttttgttggg attgcttctt tggaagcttc
      421 atgttataat taactggtta atgaaagagg agatttatgg ggactttaga aaaaaaactt
      481 gataacttag taaatactat tttattaaaa gcagaaaatc agcatgagtt attatttgga
      541 gcttgtcaaa gtgacgttaa gcttactaat acacaagaac atattttaat gttactatct
      601 cagcaacgtc tcactaatac agatttggct aaggcattaa atattagtca ggcggcagta
      661 actaaggcta tcaagagttt ggtcaaacaa gacatgctag caggaactaa ggatacggtt
      721 gatgctaggg tgacttattt tgaattaacc gagttagcta agcctattgc gtcagaacat
      781 acccatcatc atgatgaaac cttaaatgtt tacaaccgtt tattacaaaa attctccgcg
      841 aaagaattag agattgtaga taagtttgta acagtttttg ctgaggaatt agaagggtaa
      901 tccatgagat acatatcagt gaaaaatctc tcctttcaat atgaaagtga gccagtttta
      961 gaagggatca cttatcattt agatagtgga gaatttgtca ccatgaccgg tgaaaatggc
     1021 gctgcaaagt caaccttaat aaaagcaacc ttaggaattt tacaaccaaa ggctggacga
     1081 gttactattg ctaaaaaaaa taaagacggt aaacaattaa ggattgctta cttgccgcag
     1141 caagtagcta gctttaacgc tggttttcca tccaccgttt acgagtttgt caaatcaggt
     1201 cgctacccac gtagtggttg gtttagacat ttgaacaaac acgatgaaga gcatgtgcaa
     1261 gcaagcttag aagcagtcgg catgtgggaa aaccgtcata agagaattgg tagtttatca
     1321 ggtggtcaaa aacaacgtgt ggttattgcc cgtatgtttg cttctgaccc tgatattttt
     1381 gtgctagacg agccaacaac gggaatggat agcggtacta ctgatacctt ttatgaactg
     1441 atgcaccaca gtgcacatca acacgggaaa tccgttctga tgattaccca tgacccagaa
     1501 gaagtgaagg cttatgctga tcggaacatt catttagtca gaaaccaaaa acttccttgg
     1561 cgttgtttca acattcatga agttgaaaca gatgacgaaa aaggaggtca tggtcatgct
     1621 tgatatttta tcctatgatt tcatgcaacg ggcggtaatg gcggtagttg ccattagtat
     1681 ttttgctccg attttaggta ttttccttat tttacgtcgt caaagtttga tgagcgatac
     1741 ccttagtcat gtttctttgg ctggggtagc gcttggggta gtccttggta tttcaccaac
     1801 catcactact attattgttg tggttttagc tgctattttg ttagaatacc tgcgtgtagt
     1861 ttacaaacac tacatggaga tttcaacggc gattttgatg tcacttggct tggccctatc
     1921 tctgattatt atgagtaagt cgcatagttc atcaagcatg agtttagaac aatacctttt
     1981 tggatcgatc atcacgatta gtatggaaca agttgtcgcc ttgtttgcta ttgctgcgat
     2041 tattttaatc ttgaccgttc tcttcattag accgatgtac attctgacct ttgatgaaga
     2101 tactgctttt gtagatggtt tgcccgttcg cttgatgtct gttctattca atatcgtcac
     2161 tggggttgct attgctttga ccattccagc agcaggagca cttttggttt ctaccattat
     2221 ggtcttgcca gcaagtatcg caatgagatt gggtaaaaac tttaaaacag ttatcttact
     2281 gggaattgtc atcggtttta gcggtatgtt atctggtatt ttcttatctt atttctttga
     2341 aacgccagct agtgccacta ttaccatgat tttcattagt attttcctct tagttagtct
     2401 aggtggaatg cttaaaaaac ggttatttta ataagaaaaa tcctcacatg tttaacatgt
     2461 gaggatttat ttataaatgc tttaaaaagg gggtaaggtc ccaattttct tgattaatgg
     2521 tgatgctctc ccaaaagcta tcattagtta aacgaggaat caaatgaagg tgaaaatgag
     2581 tcccctcatc catcaactcc ttgtcattgc tgacgctagt gacgcctgaa atgggaaggt
     2641 gctgctctag cttagcaact agagcggctt gtaagtcgat gagatcatag cgcacttcct
     2701 ttggtagttg tgtcagacta gtgtaatggt ctttggaaat cagtaaaagg tgcccctttt
     2761 ggataggatc aatatcaaaa acaaccttga aatattgact ttgagtcagt agctgatttg
     2821 ctttgagctg atggcaaaag atacattctg tcataggtac atctccttat gaaaaaacga
     2881 gccagagctc gtttaatcgg ttatctagta agtgagaaca gcgtattttt tcttaccgcg
     2941 gcgaataacg gttaattgat catcaatttt atcatcatta cttaattggt aatctaaatc
     3001 ttgaacgcgg tctccgttaa tgtagattgc tccattttgc acgtcttcac gcgcttggcg
     3061 ttttgatggg gaaatacctg cggtgacaag catgtcaaca aggttaaggc tgtcttctga
     3121 ttgaacgtga taatttggta cattgctcaa tccttgtttt aattcattag cagaaagatt
     3181 tttgatgttt ccagcaaata actgctcggt aatgttaaga gcttgtttat aagcttcttc
     3241 gccatgaacc aaggtaacca cttcgcgtgc caaggttttt tgagcgagac gctcgtgacg
     3301 agcggcatta aattgagttt caatttctgc aatctcatct agaaataaga aggtgaagat
     3361 tttcaagaaa cgcactgcat catcatccat gacatttaac cagaattggt acatctcgta
     3421 aggagacgtc ttatcggcat caagccagac ggcattacct tctgatttac cgaatttttt
     3481 cccggttgag tcagtgatga gtggtacagt catgacgtgc ccagttttat cagccttttt
     3541 gcggagtaat tcagtaccag ctgtcatatt gccccactgg tcagagccgc caatttgtaa
     3601 ggttacatta tgcttgtcat tgagttcgta gaagtcgtag ccctgcatga tttggtaagc
     3661 aaactcagtg taagaaatgc ctgtttcaat gcgtttttta acagagtctt tactcatcat
     3721 gtagttaacg gtgaagtatt taccgacatc acggaggaag tcaataaagc tgatttgcga
     3781 gaaccagtca tagttgttga caagctctgc tttattatca ccattttcaa aatcaaggaa
     3841 agtagacaac tgccctttaa tcttgtcact ccactccaaa actgtttctt tggtttgaag
     3901 gctgcgctct gcatctttaa aggaaggatc accaattaaa cctgtggcac cacccacaag
     3961 agcgtaaggt ttgtgccctg ctagttgcaa gcgacgagat gttaagatag ccactagatg
     4021 gcccagatga aggctgtcag cggttggatc ataaccggta taataggata cttgcccttc
     4081 tgttaatgct ttgacaaggg cttgttcatc agtcgtttga aagaccaagc cacgagcttt
     4141 gagttcttca aaaatattca taggctttct ccattttcat tatagtttcg aatcattata
     4201 tcaaaaaacg ctactgctat gcaagcttta tcaaagtttg gtataatgat aaatgaggta
     4261 ttttatggtg aaatggaaca cgaaacaaaa gcgtataagt catcaaagat taggtctctt
     4321 ggatttgggg ccggtgctat tacgtacgtt gagactactg tctaactttt tttatattgt
     4381 tatcttcctt tttggaatga tgggatttgg tatggcattt gggtatttgg ctagtcagat
     4441 tgaatctgtt aaggtaccaa gtaaagaaag tttagtcaaa caagttgaat cattaacgat
     4501 gatttcgcaa atgaactatt ctgataatag tttaatttct actttagata cggatttact
     4561 tcgaacacca gtagctaatg atgcgatttc agagaatatc aaaaaagcta ttgtatcaac
     4621 agaagacgaa cattttcaag aacataaagg tgtcgtgcca aaagctgttt ttcgggcaac
     4681 attggcttct gtattgggat ttggagaagc tagtggaggt tcgaccttaa cacagcaatt
     4741 ggtcaaacaa caagttttgg gagacgatcc cacatttaag cgcaagtcta aggagatcgt
     4801 ttacgctctt gccttagagc gttatatgtc caaagacaat atcttatgtg attatcttaa
     4861 tgtttcacct tttggtcgta ataacaaggg ccaaaatatt gctggtgttg aagaagctgc
     4921 gcgtggcatt tttggcgttt ctgccaaaga tttaacggtg ccacaggcag catttttggc
     4981 ggggcttccg cagagtccta ttgtttactc tccttatttg tcaacgggac aactgaagtc
     5041 agaaaaggac atggcttatg gcatcaagcg tcagcaaaat gttctctata acatgtaccg
     5101 tacaggtgtt ctgtctaaaa aagaatacga ggactataag gcttatccga ttcaaaagga
     5161 ttttattcaa ccgggaagcg caatagtaaa taatcacgat tacctttatt acacggtgtt
     5221 agcggatgct aaaaaagcca tgtatagcta tttgattaag cgagataagg tgtctagtcg
     5281 tgacttgaaa aatgacgaga ctaaggctgc ttatgaagag agagccttaa cagaattgca
     5341 acagggtggc tataccatca ccacaaccat taataagcct atttacaatg cgatgcagac
     5401 agcggcagct cagtttggtg gcctgttaga tgatggcact ggtacagttc aaatgggaaa
     5461 tgtcttgaca gacaatgcga ctggtgctgt gttaggtttt gttggtggta gagattatgc
     5521 tctgaatcaa aataatcatg ctttcaatac aattagatcg ccaggttcta gcattaaacc
     5581 gataatcgct tatggtcctg ctattgatca aggtttaatg gggagtgcta gcgttttgtc
     5641 taattaccca acaacttact cgagtggcca aaaaatcatg catgctgata gtgaaggaac
     5701 agccatgatg ccacttcaag aggccctaaa tacttcttgg aacatcccag ctttttggac
     5761 acagaaatta ctgcgtgaaa aaggtgtcga tgtcgaaaat tatatgacaa aaatgggtta
     5821 taagattgca gactactcga ttgaaagttt acctctaggg ggcggtattg aagtctcggt
     5881 tgctcaacaa accaatgctt accaaatgct ttcaaacaat ggcttatatc aaaagcaata
     5941 tattgtagat aagatcactg ctagcgatgg tacagtcgtt tacaaacatg aaaataagcc
     6001 aattcgtatt ttttctgcag caacagctac gattttacaa gaattgttga gaggtccgat
     6061 tacttcaggc gctacgacta ctttcaagaa ccgtttggcg gctattaatc cgtggcttgc
     6121 taatgctgat tggattggta agaccggaac aactgagaat tatacggatg tttggctagt
     6181 cctgtctact ccaaaagtta ccttaggcgg ttgggcagga catgatgaca atacctcatt
     6241 agcgccatta acaggatata acaataattc taattacctt gcctatttag ctaatgccat
     6301 taatcaggcc gattccaatg ttattggagt agggcaacgt ttcaacttag atccaggagt
     6361 cattaaggcg aatgtcttga agtcaacagg tttacaacca ggaactgtta atgtcaatgg
     6421 acatactttt tctgttggtg gagaaatgac caccagtcta tggtcccaaa aaggaccggg
     6481 ggctatgact taccgatttg ctattggtgg cacggatgcc gattatcaaa aagcttgggg
     6541 gaactttggg ttcagaaaaa attaggttgc catttatcga taaaaagggt ataatagcaa
     6601 taactatttg tcgtccaatc tagctgaaat attgtccagt taggaagaac agcagttaaa
     6661 tcacactgat aaagtcagat ttagctgctc tttttgtgtc tatttttaga aaagataggg
     6721 tttgtaacct atatttaaat attctaaaaa ttaacattta gggcaaatga taacttagtt
     6781 gcgatttgct gaaacagcaa agcttaaata gaagaaggag ctaaaaactt ggcaggacat
     6841 gaagttcgat acggaaaaca ccgtacacgt cgtagctttt caagaatcaa agaagttctt
     6901 gatttaccaa atttgattga aattcaaact gactcattcc aagatttcct cgattcaggt
     6961 ttgaaagaag tatttgaaga tgtacttcct atttcaaact ttacggatac tatggaactt
     7021 gaatttgttg gttacgaatt taaagaacct aaatataccc ttgaagaagc tcgtatccac
     7081 gatgcaagtt attctgcacc aatctttgtt accttccgtt tggtcaataa agaaactggt
     7141 gagattaaaa ctcaagaagt cttctttggt gatttcccaa tcatgacgga aatgggtacc
     7201 tttattatca atggtggtga acgtatcatc gtttctcagt tggtgcgttc tccaggtgtt
     7261 tatttcaacg ataaagttga taaaaacggt aaagttggtt acggatcaac agtgattcct
     7321 aaccgtgggg cttggttgga attagagact gactcaaaag acattgccta cactcgtatt
     7381 gaccgtacgc gtaagattcc attcacaacc ttggttcgtg cccttggttt ctcaggtgat
     7441 gatgaaattg ttgatatctt tggtgaaagc gaccttgttc gtaataccat tgaaaaagac
     7501 attcacaaaa atccaagtga ttctcgtaca gacgaagccc ttaaagaaat ttacgagcgt
     7561 cttcgtccag gtgagccaaa aactgcagat agctctcgta gtctcttgat tgcgcgtttc
     7621 tttgatgcac gtcgttacga tttggctgcg gttggtcgtt acaaagtgaa caaaaaactt
     7681 aatatcaaga ctcgtctttt gaaccaaatc attgctgaaa accttgtgga tgctgaaact
     7741 ggcgaaattt tggtagaagc tggaactgag atgactcgta gcgtcatcga atccatcgaa
     7801 gaacaccttg acggtgattt gaacaagttt gtttacacac caaatgatta cgctgtggtt
     7861 actgaacctg ttgttcttca aaagttcaag gttgtgtcac caattgatcc tgatcgtgta
     7921 gtaactattg ttggtaatgc caatccagat gataaggttc gtgcattgac acctgctgat
     7981 attttggcag aaatgtcata cttcttgaac cttgctgaag gtcttggaaa agttgacgat
     8041 attgaccact taggtaaccg tcgtatccgt gccgttggtg aattgcttgc caaccaattc
     8101 cgcatcggtc ttgctcgtat ggagcgtaac gttcgtgagc gtatgtctgt tcaagacaac
     8161 gatgtgttaa caccacaaca aatcatcaat atccgtcctg tcacagcagc tgtcaaagaa
     8221 ttcttcggtt cgtctcagtt gtcacagttc atggaccaac acaacccatt gtcagagttg
     8281 tctcacaaac gtcgtttatc tgccttagga cctggtggtt tgacacgtga ccgtgctggt
     8341 tatgaggttc gtgacgtgca ttacacgcac tatggccgta tgtgtccgat tgaaacacct
     8401 gaaggaccaa atattggttt gattaacaac ttgtcttcat ttggacatct taataaatat
     8461 ggtttcattc aaacacctta ccgtaaggtt gaccgtgcga ctggtagggt aactaacgag
     8521 attatttggt tgactgccga cgaagaagac gaatacacag ttgcacaggc caattcgaaa
     8581 ctaaacgaag atggcacttt tgctgaagaa atcgttatgg gtcgtcacca aggtaataac
     8641 caagaatttt ctgcaagtgt tgttgatttc gttgacgttt cccctaaaca ggtagttgct
     8701 gttgcgacag catgtattcc tttcttggaa aatgatgact ccaaccgtgc tctcatgggt
     8761 gccaacatgc aacgtcaggc tgtgccattg attgatccaa aagcaccata tgttggtact
     8821 ggtatggaat atcaagctgc ccatgactca ggcgctgcgg tgattgctca gcacaatggt
     8881 aaagttgtct tttctgatgc tgaaaaagtg gaaatccgtc gtcaagatgg ctcacttgat
     8941 gtttaccaca ttaccaaatt ccgtcgttca aactcaggaa cagcctataa ccaacgcacc
     9001 cttgttaaag taggagacat tgttgaaaaa ggtgatttca tcgctgatgg accttctatg
     9061 gaaaatggtg aaatggctct tggacaaaac ccagtcgttg cttacatgac ttgggaaggt
     9121 tataactttg aggacgctgt tatcatgagt gagcgccttg tgaaagaaga tgtctacaca
     9181 tctgttcact tggaagaatt cgaatctgaa acgcgtgata caaaacttgg ccctgaagaa
     9241 atcactcggg aaatcccaaa tgttggtgaa gaagccctca aagaccttga cgaaatggga
     9301 attatccgta tcggtgctga ggttaaagaa ggcgacatct tagtaggtaa agtcacacct
     9361 aaaggtgaaa aagacctctc tgctgaagaa cgtttacttc acgccatctt tggggataaa
     9421 tcgcgtgaag tgcgtgatac ctcacttcgt gtccctcacg gtggtgatgg tatcgttcgt
     9481 gatgtgaaaa tctttacacg cgctaacggc gatgaattgc aatcaggtgt taatatgctt
     9541 gtgcgtgttt acatcgctca aaaacgtaaa atcaaggtcg gagataaaat ggccggtcgt
     9601 cacggaaaca agggtgtcgt ttcacgtatt gtaccagttg aagacatgcc ataccttcca
     9661 gacggaacac cagttgacat catgttgaac cctcttgggg tgccatcacg gatgaatatt
     9721 ggtcaggtta tggaacttca ccttggtatg gctgctc
//
LOCUS       DAUVUY010000068         9694 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.068, whole genome shotgun sequence.
ACCESSION   DAUVUY010000068 DAUVUY010000000
VERSION     DAUVUY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.068"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(24..734)
                     /locus_tag="VOX58_001440"
     CDS             complement(24..734)
                     /locus_tag="VOX58_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HES5878664.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(865..1362)
                     /locus_tag="VOX58_001441"
     CDS             complement(865..1362)
                     /locus_tag="VOX58_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HES5878665.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVRKDMAKLRASSLIPDDVVINGAVYDVATGKM
                     TQVME"
     gene            complement(1551..2912)
                     /gene="radA"
                     /locus_tag="VOX58_001442"
     CDS             complement(1551..2912)
                     /gene="radA"
                     /locus_tag="VOX58_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HES5878666.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            complement(2993..3439)
                     /locus_tag="VOX58_001443"
     CDS             complement(2993..3439)
                     /locus_tag="VOX58_001443"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HES5878667.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(3549..4316)
                     /locus_tag="VOX58_001444"
     CDS             complement(3549..4316)
                     /locus_tag="VOX58_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HES5878668.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWAKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(4434..6218)
                     /locus_tag="VOX58_001445"
     CDS             complement(4434..6218)
                     /locus_tag="VOX58_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878669.1"
                     /translation="MKTARFFWFYFKCYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTVTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAIVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(6221..7927)
                     /locus_tag="VOX58_001446"
     CDS             complement(6221..7927)
                     /locus_tag="VOX58_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878670.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPILFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIESGQMVGIVGATGSGKSTLAQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(7920..8369)
                     /locus_tag="VOX58_001447"
     CDS             complement(7920..8369)
                     /locus_tag="VOX58_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HES5878671.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            8667..9683
                     /locus_tag="VOX58_001448"
     CDS             8667..9683
                     /locus_tag="VOX58_001448"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HES5878672.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     HNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
BASE COUNT         2897 a         2136 c         1797 g         2864 t
ORIGIN      
        1 aagctgattt tatgttattt tgactaattt gaactagttg gtagataacg tttaagagtc
       61 ccagcaaatt gctcaaggtt aagactttgg atataaatct caccatgacc tctaaaggta
      121 ttaacaactc cttcacctgt accaattgac tgcataaagc cattttctaa atgaatatca
      181 taatcaagtt ctctgctcca tgctacaaca tgggcattat caatagtcat tgtgccacca
      241 tcaagtgtga ttttcttgat agatccaaac gagttagcaa gtaaggtacc aaggccttca
      301 gttgtcatga caaaaagtcc gccttgacca ccaaaaagtg cttttccaat attttgacgt
      361 tccattttgt actgtgctga accatctagg gctagaaagg caccatcatt tagacgatat
      421 tgtttttcac ctagttctaa agcaacaatc tgtcctggtg tattgggtgc taatgctaat
      481 ttaccatctc catttgacat agcttgtgta atgaacattg attcaccaga aaccatggaa
      541 cggccgatgg ctccaactaa ttttcctaaa ccagatccct taccatttaa tttggtattc
      601 aaggttacat tctcagtatg ataaaccata cttccttgtt gtaagtaaac tgatccccca
      661 tgttccagat caatctcaac tagtggaaat tgcatgtttt gatcaatcgt aaatctcatt
      721 ctattgtcga ccatcttact ctccttgttt gtatgaataa cttgatacaa ttattataac
      781 gcttccctat ttctttgtct aatattttca ataattttta aaaataaaaa agagctgaat
      841 tcagctctac aacttggcta ctaactattc cattacttgt gtcatctttc cagtagccac
      901 atcgtagacg gcaccgttaa taacgacatc atcagggata agagaggagg ctcttagctt
      961 agccatatcc ttgcggacac tgtcttccac atcttggaag ggaaggaaat cttgccctga
     1021 aacatccaca cctaagtgct catgaatatg cttggcaaag ccttcattag taaaagtctg
     1081 cgcgccacaa tctgtatggt ggagaaccac aatctctcgc gttcccattt gctgctgaga
     1141 aataaccagc gaccgaatca tgtcttcggt gacacgtccg ccagcattcc ttaaaatatg
     1201 agcatctcct agagccagcc ccagagcttg ggccacatgt agccgtgaat ccatacaagt
     1261 cacaatcgct actttagtct tgggttttaa gggcagatga gctgtcccat gcaaggccac
     1321 ataagcctga ttggcagcca taaaatgttc aaaataagac atcaataaac tccttttgag
     1381 aaacagcaga gctatttttg ctgttagttt gtcttatctt aacatctttt atggaaaata
     1441 gctggattaa cctcttagac ggtcggtatc gttcgaaaag ttaaaaaaac aaatgattgg
     1501 gatactttct tgacaaattt cctcctcatt tgctcttagt ttctgatcct ctagctaaaa
     1561 actgccttta aaacctgacc aaccgtagtc actccaacaa cctcaatacc ttgagggata
     1621 tcaatgcctt gcaaggcatt tttaggagca tagaccttgg taaagccaag ctttgctgct
     1681 tcattaatcc gttgctcaat acgggtcact cgccgaatct cacctgttaa cccgatctcg
     1741 cctaagaaag cttcttgagg gctggttggt ttttccttat aactggatgc gatagccaca
     1801 gcgactgcca aatcaatggc aggttcgtct agtttgacac caccagccga cttgaggtag
     1861 gcatcttgat tttgtaagag taaaccacaa cgcttttcta aaactgccat tattaggctt
     1921 acgcgattga aatcaagtcc cgtagtggtt cgtctggcat tgccaaaaac ggttggtgtc
     1981 actaaagatt gcacctctgc taagatagga cgacttcctt ccatcgttac aacgacagcc
     2041 gatcctgttg ccccatctaa gcgttcctct aaaaagactt gactaggatt taagacctct
     2101 accaagccgc cagactgcat ttcaaagatg ccaatctcgt tagtggagcc aaagcggttt
     2161 ttcacagccc gaagaatgcg aaaggtgtga tgcctttccc cttcaaagta gagaacggta
     2221 tccaccatgt gttctaacat acgaggccca gccaaggttc cttccttggt gacatgaccc
     2281 acaataaagg ttgcaatgtt gttcgtcttt gcaagctgca tcaattctgc agtgacttct
     2341 ctcacctgac tgactgatcc ttgaacacct gtaatatcag ggctcatgat ggtctgaatg
     2401 gagtcaataa ttaaaaaatc tggtttaata ttttcaattt ctgtgcgaat agcttgcata
     2461 ttggtttcgg catagaggta aaattcattg tcaatatcac ccagacgctc actccgcagt
     2521 ttaatctgct ctgctgattc ttccccagac gcatagagaa ccgttccctt attagcgagc
     2581 tgcgtagata cctgcaagag taaggtggat tttccaatac ctggatcacc accaattaaa
     2641 atcaagcttc caggaaccac accccctcca agaactctat taaattcaga catgtcagtc
     2701 tgtgttctgt gataactaat attatccaca tcttttagtt taaccggtct ggacttttct
     2761 ccagctagac taacacgggc atttttgact tctttgacct ctacttcttc cacaaaagaa
     2821 gaccaggccg agcaatttgg acagcgccct aaatactttg gcgactggta gccacactcc
     2881 tgacagataa aggttgcttt tttctttgcc atagtaatct ccctccccta gacaagacaa
     2941 acccactctt aaagaatagg tctactgtca taagcatttt tgttaaatcg ctttactgcc
     3001 ctgtcgaacc aaaaccacct gttcgttcac ctctggcttg gtcaccatca gcaattaaaa
     3061 aaggcataaa aaccccctga acgatcctct cacccacagc taatgtcaca gggtgatctg
     3121 taatgttttg catttgtgca aaaatatgtc cttcattagc ctcatttcca taataatctg
     3181 catcaatcac acctactgaa ttaatcagga taatcccttt ttttcgtgga tttgacgaac
     3241 gatcatagag gtaaagcacc tcaccatctt gcatgtaggc cttaacccct gttggtacca
     3301 acttaatctc ccctggggca atggttacag cctcagcaac agacaggtca taaccagctg
     3361 catgagtcgt ctcacgtttt ggtagtaaat caggattagt aaagcttgaa acaagctcga
     3421 aaccacgaat ttttgtcatt atgataggta tatcctttca agtcaatcat atcctcttat
     3481 tatagactat tcgtaaaaga atagcaaaaa ggcggtcaac tttaaacgga ccacttttaa
     3541 tcatgccatc acatctcatc cccacgataa ctaaagcgga tgtgggtgaa atcattatcc
     3601 aaatccttgt ctgccataaa agctttggca tcttttttca cttgaaccaa gtcaatacct
     3661 tgcattttgg cagcatagac acagccacaa tagcattggc ggtaaatgtc atactcctca
     3721 cacatttcaa cggatctgcg ataaccgttg tttttcttga aatcacttgg caggtatttg
     3781 gtggtgtaaa ccttttgcac atcgatgccc acatcattaa tggtttgcga atttttatga
     3841 ggactgatgg ttagggcact ggcaaagtag tcaaaaccta gttccactgc tttttgagcg
     3901 gtcttgtcta gtcggtaatc aaaacagaca cggcaacggt ccccaccttc tggttcttct
     3961 tctaaacctc gcacttgtcg aacgtactca ttggggacat agtccgcttc gagaaactgc
     4021 acggtgttgc ctgttttagc gttgaactcg gagacaaact gttgtgtcac ataggctctg
     4081 cgatggtatt cgtccttagg gtgaatatta gaattggcaa agtaaaccgt aatgtcagca
     4141 aactgcgtca ggtattctag ggtgtaagtg gagcatgggg cgcagcagac atgcattaaa
     4201 atactgggac gcacggattc cttagcccaa accttggcca tctgttgcat gaccctgtca
     4261 taatttatct tttgattggg attcatgtta gcgagaatct cttgtaaatc aatcattgtg
     4321 gctactttct aattgttttt tctcttgctt gctattatac catatcagga aagaaagctt
     4381 gactgcttta ttgttcacca aaaaactccg ctaagacgcg gagctgatac catctattca
     4441 aagacaaatt gattgtgata caattcggca taaaagcctt tttgatgcaa tagctcatga
     4501 tgatttcctt gctcaatgac cttaccatct ttcaacacaa taatgtgatc ggcatttaaa
     4561 atggttttga ggcggtgagc aatgacaaag ctggttcgac ctgccacgat agcttccatg
     4621 gcccgttgaa ttttactttc ggtaactgta tcaacatttg aagtggcctc atccaaaatc
     4681 aacacttcag ggtcagtcag tagtgtccta gcaatagaaa tcaactgctt ttgacctgtt
     4741 gaaaagacat tgtcatcatc tgagacatag gtattgtacc cttttggtaa ggacatgata
     4801 aagtcatgaa tatgggtcgc acgcgcagca gtttcaacca tgtcttgact aatggtctga
     4861 tcaccaaaac gaatattatc cgtaatggtt cctgaaaaaa gaactgactc ttgcaacaca
     4921 atccctacct tttgacgaag actatccaaa tcgtagtcac gaatatcacg gccatcaaag
     4981 gtaatcgaac ctgcatccac atcgtagaaa cggttaatca agttcataat agtggtcttt
     5041 ccagaacctg tcggtccaac cacggcaatc attttgccct tgggtgcaac gattgacaca
     5101 tctgataaaa ctttttgccc aggaagatac ccaaaatcga cgtggttaat tgccactgct
     5161 tcttttaagc tggtgaacgc tggtgcattt tgtggacgaa cttcttcggt ttcatcaaac
     5221 atttcttgaa tacgatgagc accggtaaag gccagctgca attctcccca actagacgcg
     5281 atttgcatca tgggttggta atattgctgg gaatattgta caaaagtaac cactaaacca
     5341 agcgctgccg ctgctggcat agatttgtca ctgaggacaa ttgttgaacc gacaaaaatc
     5401 acgatagccg tgttaataag gctcattcca ttcatgactg gaaataattg tcctgagaac
     5461 agacggcgtt tgaaggtggc ttgtcgaacc ctttcgttat gttttaaaaa ggctgtcacc
     5521 gtatcttctt ggacaccttg tacaataata gccttttgtc ctgaaatggt ttcatccata
     5581 aaagcattta aagcactgac ttcttgctgt tggatattgg tgtattttct tgccaaacgg
     5641 atgttaatca ctaaaaaaat gagagcaact ggggttgatg cgatggttaa caaagctaaa
     5701 cggctatctt gcctaaacat catccagacc aggccgatgt aaagggcaat attagtcacc
     5761 acttgaacca aggattggtt cagcgagttt tgaatattat ccaaatcact cgtgaaacga
     5821 gaaagaatct ccccatcttt atggcggtca aaaaaggcga cggttaaacg ttctaattta
     5881 ccaaacaagc ccttgcgcat gcggttggtc gaatgtgaga caacacgtgt gagtaagaaa
     5941 ctatatatta gattagctaa aacagtaaag aaataagtca tcaaaagctt aaacatcacg
     6001 gcattaaaag ctgataaatc agggctaaaa tgtgtctgac cactcatctt agcaacgtaa
     6061 taagcctgac cgatttttcc caactcagtc aaggactccc ctaagaagac aggagctttt
     6121 acttgtaaat aagttgctaa gataacggca acagcaatga cagtaaacga gaaacgatag
     6181 catttaaaat aaaaccagaa aaaacgtgct gttttcatca ctactcctcc cttccttttt
     6241 gtgtctcata aatttcacga taaatcgcat tattagccac caactctgcg tgtgttcctt
     6301 ctccaattaa ctgtccttgg tcaaggacta agatcttatc tgccttaact accgatgaaa
     6361 ttttttgagc aacaataact gtagttgtac cttctaattt atgacttaag gcttcttgaa
     6421 cgcgtttttc agattttgca tcaagagctg aagtggaatc gtctaatata agaattttgg
     6481 gatgattaat gacaccacgt gcaatagaca gacgttgctt ttgtccgcca gacaagttac
     6541 tacctcgctc ttcaacttgg ctctcataac ggctatccat acgatcaata aattcttttg
     6601 cttgcgcgat ttgagcggct ttttgcatag catcaatatc agcttttgct gagccttggc
     6661 gaaggttatc agctatcgtt cctgaaaaga gaatagcctt ttgcaacacg atggaaaccg
     6721 attggcgcag ggttgtttga cttagggttt taatcggctt tcctcctaac aaaatttggc
     6781 catcttgagg atcaaataag cgagggatga gctgtgcaag cgttgatttc ccagatcctg
     6841 ttgcaccaac gatgccgacc atttgcccag attcaatagc aaaagaaatg tgcttcaagg
     6901 ttggctcatc atcattagga tatgtaaaac taacatcgtc aaatacaatg cttcctgaga
     6961 tggtttcttc cttttcattt tcaaatgtca tggcaggttc tgtagacaat atttgcctaa
     7021 ttctagccat agaaacaaaa gcacgactaa cttgcatccc catggaacca accacaataa
     7081 ttgaaaacat aatttgcatc atataagtca tgaaagaggc aatattccca atcactgttg
     7141 gatctgtttc gaccatggtc gaaacaacat ttatagaaac ataaactgcc aaataagaca
     7201 ccaacattaa agccggctgc attagtgaaa aaccataacc aatagataaa tttaacgcca
     7261 aaagatcatt ggacgtttct ttaaatttag tgtattgttg ttgttcttgt acaaatgatt
     7321 taaccacgcg aacgccacgg agattttcct tagcgatgcg attaatctta tccattaacc
     7381 gttgaaattt accaaaacga ggacccattt gacgcatcac caaacccatg atgagggcaa
     7441 ttaagataac cattacaaca atgacccacc ataattgagg gaatgtctga actgccataa
     7501 taaacgcgcc aataaaaagg atcggcaaac gaaagagaat ttgaaacatc atcatgacta
     7561 aactttggat ttgattgata tcattagtta gcctaaccac aagattacca gcattaaaag
     7621 cttcaatatt ggcatatgaa aagtcttgga tttttcgaaa ggtcttctcc ctcatatcag
     7681 cagatacacc ttgagctatt ttagcagcta aaaccgtatt aatcgctcca gacattaacc
     7741 ctaaaagggc aataataagt aatggtttcc caacctcata aattttctct tgattatttt
     7801 gaatcacagc gcttagcaca gatttaagat aatgtggttg caagagacta cttgcaacaa
     7861 ccccaataac cataaaaaat gagcctaaag cataccattt ataacgtaag attgcgtctt
     7921 taagcatcgt tgtctccttt ataagttagc atattttgct tgagctgttc aattactttt
     7981 ttcgctgtca gcaactcttc ttttgtaatt tcttttaaaa aatagtgctc gatatcctta
     8041 cgacactccc gcaacaaagg caatttatct ctaccaacct gcgctaacac tacttgctta
     8101 taacgcttat ccacttggga aggcatcaca ttgataaacc catttttgac catacgtttc
     8161 actaaatgac tagcaactga ctttgagata cgaagttgtt tttcaatatc tttgacaaat
     8221 atttcttgat tttggtgttt agctaaaaaa acaagaacat aaccttgagg acctgctaga
     8281 tgttctacat catatttttt tgcaatctca tcactaattt gttcgatttg atgtatcaat
     8341 tcacgtaaat cacctatcac ttgtgacatc tcttccctcc ttattttctt taatagtttc
     8401 tatgataagt attttagttc tctagataac tattgtcaat taaaatatct ttatttttaa
     8461 aaataagttt ccgaacttaa ataactaaaa agaacagggc aaagccatga caacagctca
     8521 cctgatgaag cttattaaac aacattatta atatagctag ctctcttggg aacgccttat
     8581 tagccaagac catatacctt ttctaataga gctaatcaag gtgttatgtt ataatagagt
     8641 gactagctct tgaaggagaa tttagaatga caaaacaaaa agtagctatt ttaggacctg
     8701 gttcttgggg aactgccctt tctcaggttc ttaatgacaa tggccatgac gtccgtttat
     8761 ggggaaatat tcctgatcaa attgaagaga taaataccaa gcataccaat agacactact
     8821 tcaaagacat tgtgttagac aaaaacatta cggcaactct tgatttaggt caagccctat
     8881 cagatgttga tgcggtgctt tttgttgtgc caactaaagt aacacgatta gttgctagac
     8941 aagttgccgc cattttagat cataaagtag tggtcatgca tgcctcaaaa ggtctagaac
     9001 cagagaccca cgaacgttta tcaactatcc tagaagaaga aattccagct cattttcgta
     9061 gtgaagtggt cgttgtatca ggtcctagtc atgctgaaga aactatcgtt cgcgatatta
     9121 ctctcatcac ggcagcctca aaagatattg aagctgcaaa gtatgttcag tccctcttta
     9181 gtaaccacta cttccgactt tataccaata ccgacgtcat cggagttgaa acagctggtg
     9241 ccctcaaaaa cattattgct gttggagcag gagctctcca tggacttggg tatggagata
     9301 atgccaaagc agcggtaatt actcgtgggt tagctgaaat cacccgtcta ggcgtcaagc
     9361 ttggtgctga cccgttaacc tacagtgggt tatctggtgt tggtgatcta attgtaacag
     9421 gtacttctgt ccattcacgt aactggcggg ctggagctgc ccttggacgt ggtgaaaaat
     9481 tagaagatat tgaacacaac atgggtatgg ttatcgaagg catcgcaaca actaaggtag
     9541 cctatgaaat tgcccaagac ctcggagttt acatgccaat tacaacagct atttataagt
     9601 ccatctacga gggcgctgat atcaaagaaa gcatcctcgg catgatgtca aatgaatttc
     9661 gttctgaaaa tgaatggcat taagccattc gtca
//
LOCUS       DAUVUY010000069         9688 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.069, whole genome shotgun sequence.
ACCESSION   DAUVUY010000069 DAUVUY010000000
VERSION     DAUVUY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.069"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..452
                     /gene="pheS"
                     /locus_tag="VOX58_001449"
     CDS             <1..452
                     /gene="pheS"
                     /locus_tag="VOX58_001449"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HES5878673.1"
                     /translation="GRVFRRDTDDATHSHQFHQIEGLVVGKNISMGDLKGTLEMIIKK
                     MFGEERSIRLRPSYFPFTEPSVEVDVSCFKCGGKGCNVCKKTGWIEILGAGMVHPSVL
                     EMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRGFYQGDQRFSEQFN"
     gene            662..3067
                     /locus_tag="VOX58_001450"
     CDS             662..3067
                     /locus_tag="VOX58_001450"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HES5878674.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSATSIEVEGIEVPADGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANS
                     QTVVLEVAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNELNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLASMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALPSDQTFAEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTKIKLFDVYEGVTIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            3177..3554
                     /locus_tag="VOX58_001451"
     CDS             3177..3554
                     /locus_tag="VOX58_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HES5878675.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVMVTITLEGLSHV"
     gene            3547..3933
                     /locus_tag="VOX58_001452"
     CDS             3547..3933
                     /locus_tag="VOX58_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HES5878676.1"
                     /translation="MSDAGLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            4006..5082
                     /locus_tag="VOX58_001453"
     CDS             4006..5082
                     /locus_tag="VOX58_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878677.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSILLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GISNKTITTAVLMQTFFLSFLGSGLGLLGTWLTSLLLPTVVPFQSNWFLYLAIFVSMI
                     CFALLGTLFSVFNIIRIDPLKAIG"
     gene            5092..5760
                     /locus_tag="VOX58_001454"
     CDS             5092..5760
                     /locus_tag="VOX58_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878678.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGITGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYRMQDGEL
                     RQER"
     gene            complement(5865..6782)
                     /locus_tag="VOX58_001455"
     CDS             complement(5865..6782)
                     /locus_tag="VOX58_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HES5878679.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRTVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
     gene            6933..>9688
                     /gene="rexB"
                     /locus_tag="VOX58_001456"
     CDS             6933..>9688
                     /gene="rexB"
                     /locus_tag="VOX58_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HES5878680.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVIVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFFKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELISFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQ"
BASE COUNT         2983 a         1737 c         2009 g         2959 t
ORIGIN      
        1 caggtcgtgt tttccgtcgt gatactgatg acgctaccca ctcacatcaa ttccatcaaa
       61 ttgaaggttt ggttgtgggc aaaaatattt ccatgggaga cctcaaaggg actcttgaaa
      121 tgattatcaa gaagatgttc ggtgaagaac gtagcattcg tttgcgcccg tcatatttcc
      181 cgttcacaga accatctgtt gaagtggatg tttcttgctt taaatgtggt gggaaaggct
      241 gtaatgtctg caagaaaaca ggatggattg aaattcttgg tgctggtatg gttcacccaa
      301 gtgtcttgga aatgtcaggt gttgatgcaa aagagtattc aggctttgcc tttggtttag
      361 gtcaagagcg gattgccatg ttgcgttatg gcattaatga cattcgtggt ttctatcaag
      421 gcgaccaacg tttctctgaa caatttaact aaaatgagtc gtttgaagac ttctgtcagc
      481 tattttatct aagattgagt gaaagttgat aggtaatgat aataggttaa ttgtctaagg
      541 ggaaagtccc tctcgcttca acgtcataag ggttttgact tggaacttaa aatacgaaag
      601 gaaatgactc aaatcacggc ttgagagctt tctctagccg agtgatttgg gcactaaaat
      661 tatgttagta tcttataaat ggttaaaaga attagtagat atcgatgtga caccagcagc
      721 acttgctgag aaaatgtcgg caacgagtat cgaggtagaa ggaattgaag tccctgcaga
      781 tggtttatca aaactggttg tggggcatgt gctttcttgc gaagatgtgc cagagacaca
      841 tttgcatttg tgtcaggtag atacaggtga tgaaacgcca cgtcaaattg tgtgtggcgc
      901 accaaatgtg aaagcaggga ttaaggttat cgtggctgtg ccaggtgctc gtattgcgga
      961 taactataaa atcaaaaaag gcaaaatccg cggcatggaa tctcttggca tgatttgttc
     1021 cttgcaagaa cttggtttgt cagatagtat tattccaaaa gaattttcag atggtattca
     1081 aatcttgcca gaggaggctg tgccaggtga tgctattttt aaatacctgg acttggacga
     1141 tcatatcatt gaattgtcca ttacacctaa ccgtgctgat gcgctttcta tgcgaggtgt
     1201 ggcacatgaa gtcgcagcta tttatggcaa gtcagtatca ttcccccaaa aaaatctaca
     1261 agaatctgat aaggcaactt cagaagccat tgaagtggcc attgcctctg ataacgttct
     1321 aacttacgct agccgtgtcg ttgaaaatgt caaagttaag ccaagtccac aatggttgca
     1381 aaacctttta atgaatgctg gtattcgtcc tattaataat gtggttgatg tgaccaacta
     1441 tgtgctcttg tattttggtc aacctatgca tgcttttgat tatgataaat ttgaagacca
     1501 taagattgtg gcgcgtgcgg ctcgtcaagg ggaaagttta gtcacacttg atggtgaaaa
     1561 acgggatttg accactgagg atcttgttat taccgtggct gacaaaccag ttgctttggc
     1621 aggtgttatg ggtggtcaag ccactgaaat tgatgccaac tctcagacgg ttgttcttga
     1681 agttgctgtt tttgatggca aatcgattcg taaaaccagt ggtcgtttaa accttcgttc
     1741 ggaatcctct tcacgttttg aaaaaggggt taactatgca actgttttag aagcgcttga
     1801 ctttgcagcg gctatgttac aagaattagc agaaggtcaa gtcttatcag gccatgttca
     1861 agctggacaa ttgccaacag aacctgttga agtctcaact agccttgact atgtgaatgt
     1921 tcgtttagga acagaattaa cttttgctga tattcaaagg atttttgacc aacttggatt
     1981 tggtctaaca ggagatgaaa caagcttcac ggtagctgtt cctcggcgtc gttgggatgt
     2041 tagtattccg gctgatttgg tggaagaaat tgcgcgtatt tatggttatg ataagttgcc
     2101 aacaacctta ccagaggcag gaggaacggc tgcagaatta acgccaacac aggctcttcg
     2161 tcgtaaggtc cgtggtttag cggaaggatt gggcttgact gaaatcattt cgtatgcctt
     2221 gacaacacca gagaaagctg ttgagtttgc agtggcacca agtcacctaa cagaactcat
     2281 gtggccaatg tctgtggaac gttcagcgct tcgtcaaaat atggtttcag ggatgttaga
     2341 tacggtagct tataatgtgg ctcgtaaaca aagtaacttg gctctttatg aaattggaaa
     2401 aatctttgaa caagaagcta atccaaaaga agaccttcct aatgaattga accattttgc
     2461 ttttgctatt tgtggcttag tggcacaaaa agatttccaa acccaagctc aggcagtgga
     2521 tttttaccat gctaaaggta tcttggatac tttatttgca aaccttaacc tcaaggttca
     2581 atatgtgcca accaaagacc tagcaagcat gcacccagga cggacagcgc ttatcctatt
     2641 agatgagcaa gtgattggat ttgttggtca agttcatcca ggaacagcaa aggcttatag
     2701 tattccagaa acctatgtgg cagaattgga tatggctgct ttagaagcag cgttgccaag
     2761 tgatcaaact tttgcggaaa tcacaaaatt ccctgctatg acacgtgatg ttgccttgtt
     2821 acttgaccgt gaagtgagtc atcaggccat tgtaacagct attgaatcag caggtgtaaa
     2881 acgcttgact aagattaagc tctttgacgt gtatgaagga gtaactattc aggctggcaa
     2941 aaaatcaatg gcttatagcc ttactttcca aaatccaaat gacaatttaa cggatgaaga
     3001 agttgccaaa tacatggaga aaatcaccaa agcattgaca gaacaagtcg gtgctgaagt
     3061 acgctaagaa aaaaactggg cctctaaggc tccgtttttt tctgttataa tagaggaaaa
     3121 aagaactcca gttcaaatga ttggagaaaa acatcagaag gtggaggaaa atcagaatgt
     3181 atcaaacaaa aataacaggc aaccgccttt accacacggt ttcgaaaggc tacggagata
     3241 gtgtaacctt atttggaaaa actgatcaag gggaaacccc tatgagttta ctggttattg
     3301 ccttgtcttc ttgtgtgact atgtgtgtgc agggttttta ccatcgtcaa tttcagcaag
     3361 acacacttgc tgtttctgtt gaggctagtt atgaggaaga agtttttgaa ctacttgtta
     3421 ggattgatga tgttttagat gacaaaaaga aagctgattt gttggcttac attggaactt
     3481 attgtcgtgt gaaacaactc ctcaaacctg aagttatggt gaccattacg ttggaaggac
     3541 tgtctcatgt ctgatgctgg tttgaaaact taccaaaaac aatgggctta tcaaaagtat
     3601 tgggtcatgg cacactcaca gcagcattac aatgctttaa gagaattgtt taaaggaaat
     3661 cagtggtcag aagaaaaggt gttgactttt cattgtttga ttgaagaagc gcaagccatt
     3721 cctcccacag taaaaagctt acgaacagca tatcagcatg tttgggggta ttttaaaaag
     3781 gtagcgagtc aggaagaaaa agaccatttc aaagatttgg atgctcagtt ggaaactaaa
     3841 tctgaggaga tgctatgctt tttacaagag atgacggctc attatcagcc ctcttatctg
     3901 ctatcttgcc gtttgattac caaaggtcct taaatgctaa acagaaaagt agggacttct
     3961 gggaagttat gctaagctag tgatataaac aagggggaat gagagatgtt tttagcgctt
     4021 aatgaaatga aacaatccaa attgcggtat ggtttgattg ctggtttgct atgtttggtt
     4081 gcctatttga tgttcttttt gtcaggattg gcctttggtt tgatgcagga gaaccgctcg
     4141 gcggttgatc tttggaaagc tgacagtatt ttactagcca aggatgctga cgctaccttg
     4201 accttatcac aggtgtccag agctcaagaa aatcaaataa cagcagacaa ggtagctcct
     4261 cttgctcaac tcaataccgt ggcgtggtca gttaaaaatc ctaaggatgc cgacaaagtt
     4321 aaggttagcc ttttcgggat tgattctaat agctttattc gtcctaacat tgtaaaaggt
     4381 cgattattta agactaacaa agaggttgtt ttggatcaaa gccttgcaaa agaggaagct
     4441 tttgcgattg gcaaggactt ttacacatcg agttcgagtc aagcattaac tatcgttggt
     4501 tatactcaaa atgctagatt tagtgttgca ccagtggttt atatgaatct ggaagctttt
     4561 gaaacattaa aatatggaga accactacca aaagataagc aagttgttaa tgcttttatc
     4621 actaaaggaa gtttaacaga ttatcctaaa aaagacttcc aaaaattaga tattaaaacc
     4681 tttattacta aattacctgg ttatagcgct caacttttaa cgtttggctt tatgattagt
     4741 tttcttgtca ttatttcagc tattattatt ggtattttta tgtatatttt gactattcaa
     4801 aaggcaccta tttttgggat tatgaaagcg caaggaattt ctaacaaaac gattacgacc
     4861 gctgtgctta tgcagacatt ctttttgagt tttttaggta gtggtttagg gttgctaggt
     4921 acttggctga catcattatt attaccaaca gtagttcctt ttcaaagcaa ttggttttta
     4981 tatttggcta tattcgttag tatgatctgt tttgctctat taggcacctt attttctgtt
     5041 tttaacatta tacgaattga tcctttgaaa gcaattggat aggaggtagt gatgtcggtt
     5101 ttaacattta aacaagtgac caaaacattt caagatggtc atcacgaaat caacgcatta
     5161 aaggcaactg acttttccat tgaagctgga gaatttgtgg ctattattgg accatcaggt
     5221 tctggaaaat caaccttttt gaccattgct ggtggcttac agaccccaag ttctggtcag
     5281 ctaatcattg atggcactga ttatacgcat ttatcagaaa aagagcgctc acgcttgcgc
     5341 ttcaagtccg ttggttttat cttacaagct tccaatctga tacctttttt aacagtccag
     5401 cagcagttgg aattggtcga tcatttgaca ggaagcaaag aaaaagccaa agccaatcag
     5461 ttatttgacg atttaggcat tacagggcta aaacatcagt tacctcaaga attgtccggc
     5521 ggagaaaggc aaagagcggc gattgcccgt gctctctacc atgatccagc actgatattg
     5581 gcagacgaac ctactgcaag tttggatact gaaaaagcct atgaagtggt caaactgtta
     5641 gctaaggaaa gtaaagaaaa aaacaaggcc attattatgg tcactcatga tgatcggatg
     5701 ctaaaatatt gtgacaaagt ctatcggatg caagatggag agttacgtca agagagatag
     5761 tagactggta agagtttatg atattagaag aaaaaaacca aacaagcaca gcacttgttt
     5821 ggtttttttc taagtgtgac tattatcaat acgtcaccat ttccctaaag cttactatac
     5881 gggatgctaa aggtatcacc atgctcaaag tcgccatatt ggtaacctcg gtcaaaccaa
     5941 cgttggcgtt gcttagaagt gccatgtgta aaactatcag gaacggttct gccataggtt
     6001 tcttcttgca gagtatcatc cccaactgcg tgagctgctg ccattgcttc ctcaatatct
     6061 cctttttcca aaaggccttg cccttgaaca taattagccc aggcaccagc atagtaatca
     6121 gcttgtaatt ccaacttgac gttgagctga ttggctttag cctttgattt tccttgtcta
     6181 gcgctagcgt aattatccat aattcccaat tcattttgga tgtggtggcc aacttcatga
     6241 gcaatcacat aggccatagc aaaatcacct tttgcgccgt acttggttga tagttcatta
     6301 taaaaagaaa tatctaaata cactttttga tcgcctgggc aataaaaagg accagaagag
     6361 gcttgacccc tgccacaagc ggtttgagtt gctccagtat atagaactaa tgttggttta
     6421 tgataagtta accctttctc acgaaatgtc ttagtccaat agtcctcggt agaggcgaag
     6481 accttactga caaaagaaac ctgttcttga ctagccttgt caccatttgt acgcgttact
     6541 tggcttgatt gataggcatt gttgttggta gaagaaggct tgcctcctaa aacaccagat
     6601 aggccacctc ctcccataac taggagaagg agaagaataa ccagcttggt tttccaacct
     6661 cctcgtgaaa agagtaattg aagcaggaga ccgcccccta ggccgccgcc cccaaaagaa
     6721 ccagacgatt gaccacgtct atcttcaacc tgttgacttt ctctcaaatc atccgttttc
     6781 ataagtttct cccatctatt tcttgatttt attataaaat aattctatta gaattgctat
     6841 ttttatgatg agattataac taaaaatagg aaaagcaata ggtatttgct ataatataac
     6901 aagggtagat tcagtttgta taaaggagtc atatgaaatt aatttacaca gagatgagtt
     6961 actcgatgac agagattttg gtgaatgaag cacgaaaagc tgctgaccaa ggttatcgag
     7021 tgttctatat tgctccaaat tctctttcat ttgaaaagga aagagaagtc ttaaccttat
     7081 tgcctgaacg agggactttt tcgataatag taacccggtt cgttcaaatg tcaaggtatt
     7141 ttacagttga gagctctcct tctaagcaac atctagatga cacgactttg gctatgattt
     7201 tttatcgggc cttgatgcaa ttaaaaccag aagatttgcc gagttatggt cgtttgcaaa
     7261 ataattcggt ttttattgaa caactagttg agttatataa ggaattaaaa aacgcacaat
     7321 taagcgtaca tgatttaaca ggattagacc atcctcaaaa acaagaagat cttattaaaa
     7381 ttattgagct tgcagagacg atcatgattc agcaagatta taatcaagat agtcctctac
     7441 aatcttttgc aagagcaatt aagttaggac ttttaaataa ccaactatca aaaacagtta
     7501 ttgttattga tggtttttca cgtttttcag ctgaagagga ttatttatta tcacttttaa
     7561 ataacaactg tcaagaggtc atcattggca gttatgtgag tcaaaaagct tatcaaaaat
     7621 cttttattaa gggaaatatc tatgaggcta gccttcattt tttgcaagat ttggctcaaa
     7681 aataccatat caagccggta tttgcgacct ctaatcaagt gtttaaacca gccttttcac
     7741 ggctaacaca attatttgaa gcaactcatg atttttcaca agtagactgg caactccaaa
     7801 agaatgatct tgatcacttt agcctttggc aatgtcatca tcaaaaagag gaaattgaac
     7861 atgtagcaaa atccattcgg caaaagctat atgaaggtta ccgttataag gatatcttag
     7921 tgttgctagg agatatggat gcttatcaat tacaaattgg gcctatcttt gataagtttg
     7981 aaatccccta ctatttagga aaagcagagc ctatggctgc ccatccatta gtccaattta
     8041 tagaatcgct cgaacgaagt cagcgctata attggcgtcg tgaagacatt ttaaacatgt
     8101 taaaatcagg actttttggt tgttttgatg atagtgatat tgatcgcttt gaggaatata
     8161 ctcagtttgc agatattaaa ggatttacta aattttcaaa gccttttacc attaactctt
     8221 ctaggcagta tcctcttgac tttttaaatg aaatgagaca agacatcgtt ttacctttgc
     8281 aagaattgtt taagagtcaa aaacaacttg gtgcttcact tgttgacaaa ttaatacttt
     8341 tttttaaaaa gattagatta gctgagaata tgcaaggttt agcacagtcc caattagagg
     8401 tggaaaaaaa cgaagaagtt tggaaaaggt ttacagatat tttgacttct tttcatcata
     8461 tctttggcca agaaaaatta cggctatcag attgccttgc tttaatcaaa acgggaatga
     8521 aatcagctca gtatcgtgtt gttccagcga cattagatgt tgtcactatt aaatcttatg
     8581 accttgttca accacattca aaacctttcg tttatgcaat tggtctaact caatcacatt
     8641 ttcctaagca aattcaccat tcaggactac tttctgatca agagcgtgct agaattaatg
     8701 aaataagaaa ttatcgtcat tttgatattg ctagtgccga aaatagtaaa aaaaaccatc
     8761 aaactgcgtt atctctgttt aatgctgcta ctaaagagtt agttcttagt gttccaacgg
     8821 taatcaacga gacattcgat gatctatctc cttatcttaa agaattaata agctttgggc
     8881 tacctctttt ggataaaggc aaaaactatc tatcttatga taatagcgat attggtaact
     8941 ataaagctct cttgtcacaa attattgcta ttaaccgaca agatcttata gaaatgtcag
     9001 atcaagataa gatgttttgg actgttgtct tacgttatct caggaaacag ttaagaaagc
     9061 aacagctaga gctacctact tcagattatc gtttatcaac aaagcctttg tctaaagaag
     9121 ttatcgaggt atgttttcca aagggaatcc ctttaaagct atctgcaact gctttaaccg
     9181 ttttttataa taatcagtac aactattttt tgaaatatgt tttgaattta aacaaaacag
     9241 aatccattca tccagattcc agaattcatg gtcaatactt gcatcgtgtc tttgagcggt
     9301 taatgaaaga tcacacgcaa gaaccgtttg ataataaatt aaaacaagca atctaccata
     9361 ccaatcaaga aagttttttt cagcaggttt accaagataa tgccgaggct gagtacagtt
     9421 tagctatact agaagatatt gttagatcaa cagcacctat cttgcaactt aaccaaaaca
     9481 taaaggtcat tgatcaagaa aagaactttc atctggatat gggaaatgag atattagtcc
     9541 acgggatcat tgatcgtatt gaccagctga gcgacggtag cttgggcatt gttgactata
     9601 agtcaagcgc gaatcaattt gatattggga ccttttataa tgggttgagt ccacaactag
     9661 tgacttacct cgcagcctta aaacagat
//
LOCUS       DAUVUY010000070         9539 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.070, whole genome shotgun sequence.
ACCESSION   DAUVUY010000070 DAUVUY010000000
VERSION     DAUVUY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.070"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            247..726
                     /gene="rlmH"
                     /locus_tag="VOX58_001457"
     CDS             247..726
                     /gene="rlmH"
                     /locus_tag="VOX58_001457"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HES5878681.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(884..957)
                     /locus_tag="VOX58_001458"
     tRNA            complement(884..957)
                     /locus_tag="VOX58_001458"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(921..923),aa:Arg,seq:tct)
     gene            1122..1896
                     /locus_tag="VOX58_001459"
                     /pseudo
     CDS             1122..1896
                     /locus_tag="VOX58_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2091..2162
                     /locus_tag="VOX58_001460"
     tRNA            2091..2162
                     /locus_tag="VOX58_001460"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2124..2126,aa:Glu,seq:ttc)
     gene            2196..2269
                     /locus_tag="VOX58_001461"
     tRNA            2196..2269
                     /locus_tag="VOX58_001461"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2230..2232,aa:Asn,seq:gtt)
     gene            complement(2766..5342)
                     /locus_tag="VOX58_001462"
     CDS             complement(2766..5342)
                     /locus_tag="VOX58_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HES5878682.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLTSMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKINAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLITENKTRTYYLSISLLFIQNYYFGYMIALFCILYALVCLLSLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFSFRNSQIPPTFISAFILIFSLLES
                     GLNTYYQLQGINKEWGFPSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDS
                     MKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSE
                     GPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSG
                     KSLTYFNLQPAQLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIF
                     SNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHF
                     YSLSIESYLEAMNSIKQKNVHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGK
                     NLPVKKAQGGFLSVTIPKGKGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSK
                     LL"
     gene            complement(5425..7044)
                     /locus_tag="VOX58_001463"
     CDS             complement(5425..7044)
                     /locus_tag="VOX58_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HES5878683.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVIVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(7123..7995)
                     /locus_tag="VOX58_001464"
     CDS             complement(7123..7995)
                     /locus_tag="VOX58_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HES5878684.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    8118..8359
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8414..9436
                     /gene="trpS"
                     /locus_tag="VOX58_001465"
     CDS             8414..9436
                     /gene="trpS"
                     /locus_tag="VOX58_001465"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HES5878685.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
BASE COUNT         3303 a         1545 c         1717 g         2974 t
ORIGIN      
        1 cttaaggatt ttaagatata tttcatactt ggcatgtttt gtcctccgaa ttatttgtcc
       61 atagcttata gaactttgct taattatacc ttaaaaataa aaaaaagaaa agtaatccac
      121 aggctgtgga ttacttttct ttttactaat aaagaccttt gtacaaaggt ttttattagt
      181 agttgaagaa aaataacttg tggataaatt gtgtaaaaag taatgttctt gtgatacaat
      241 taagctatga aagtgaaact aatttgtgtt ggaaaactaa aagaaagata ccttaaagat
      301 ggtatttcag aatatcaaaa aagactttcc cgtttttgtc aattcgaaat gattgagtta
      361 actgatgaaa gaacaccaga taaagcaagt tttgctgata atcaattgat tatgagtaaa
      421 gaagctcagc gaatccataa aaaaattggg gaacgtgatt ttgtaattgc attagctatt
      481 gaaggaaaac agtttccttc agagacattt agtgaattaa tatcaggggt tactgttaag
      541 ggatattcaa ctatcacgtt tattattggt gggagtcttg gattagattc aattattaaa
      601 aaaagagcta atatgctaat gagttttgga ttgttaacct tacctcatca gttaatgcgt
      661 ctagttctta ctgagcaaat ttatcgtgct tttatgatta cacaaggcag tccttatcat
      721 aagtaagaga gtgtgaactg gaataactaa ctcttaccac tttattagat aatagttcgg
      781 tataattaaa agcaaatatt aaaaaaagac gtactaaatc ctatcttttc aatacactaa
      841 ggtaaataca aaaaaaagca ctaaaacagt gcttttttat ctttgatccc agcaggattc
      901 gaacctgcga ccgttcgctt agaaggcgaa tgctctatcc agctgagcta tgagacctta
      961 accacatcat tttaacaaaa attatctaaa actgtcaata tttattttga aaaaactaga
     1021 aaataacata atatcccgcg ttgtaaaatg aagtgcaaca aaaaagacat gttcatctga
     1081 tatactagaa ttccccaatt cagtatggaa agcagatgaa catgtctaat attaattcta
     1141 ccagaaagtc atcctattct catctttcag ccacagaacg gggtgaaatt gctgcttatc
     1201 ttaaaatggg aaagaaaccc gttgagattg ctcgacttct ggggcgtcac tgttctacaa
     1261 tctgccgaga aataaaacgt ggttcagtag atcaggtaaa ggataagaat ggaaaacaaa
     1321 ccttcttcaa cgcctatttc gctgatagtg ggcaacgtgt ctattaaacc aatcgatttg
     1381 cctaaggtgg ttagtatcag aaaacgttct aagaaagtca tgaagacgaa taagaagact
     1441 ttaggtaaat ctatcgaaga acgtccagaa tatattaatg atcgttctga atttgggcat
     1501 tgggagattg atttggttct cggcaagaaa accaaaagtg aagctgttat gttgactttg
     1561 gtagagcgcc agacacgcta tgcactagga gttaagctag aagacaagca gtcccaaacc
     1621 attaaccgtg ctgtcacgca tctcatcagt cagtatccta ttgcatccat cacagcggat
     1681 aatggttctg agtttagttt actctcagac ttaaaagctg ttgacgttta ctttgcacat
     1741 ccctattctt cacatgagag aggaacaagc gagaacttca atggcctcct cagagaatat
     1801 gtccctaaag gagtctcact taatccacta acctctgaag aacttgacaa tgatattact
     1861 gccatcaatg agcgcctaag acgacttctt caatagcaat cctcaaaatt cctgtttgag
     1921 ctatcccgaa cagcttaacc tctggaactc tagttatcta tgaactcgtt gcacttgact
     1981 tgacaacttg cggtttaaaa aaaatcaaat ttttttaaaa aaagtataga caaaaaaaat
     2041 caatctgcta taatagtaag agtgttaagg agatatcctc caaaacttct ggtccgttgg
     2101 tcaaggggtt aagacaccgc cttttcacgg cggtaacacg ggttcgaatc ccgtacggac
     2161 tatcgcaatt gtcattttgg caattttttt ctttttccgg catagctcag ttggtagtag
     2221 cgcatgactg ttaatcatga tgtcgtaggt tcgagtccta ctgccggagt gataagatat
     2281 ttcctaagat aatcttagga tttttttatt tagtaaaaca gatactcagg tcaaatactc
     2341 aatttactaa ttattaaacc actaaataca gcattgtgaa ctaacaggat tgtattttgc
     2401 ataaaaatag aagttctgtt ctttaattgt atagatgata acattaagtt taattacaaa
     2461 cattaactga agcttaattt tttacatttt gaaagcatgt ctcccattta atactgatga
     2521 atataagatt attgtaagga gtcaaaagat tgaaatcgta tcagcgatga ttattgtcat
     2581 ttcaatcttt gaaagagagt agtttaacat taaccctcac gaaaaatctg acctttatat
     2641 tatttgagtt tattagtaaa atcgataaaa gcatctcaag tattagaata tttgtctagc
     2701 tcttgagatg cttttagtat attgactcat ttgccaaatt gataaaaaaa tctgattttt
     2761 ttggactaaa gtaatttaga ctttatatct atgtacttat acaaaagcat ataagcgata
     2821 attcctacgc aagatagaga taaccctaat ttaaaaccat taggaataaa ggtaaggata
     2881 acacgtccct ttcctttagg aatagtaact gatagaaatc ctccttgtgc ttttttgact
     2941 ggaagatttt tcccatcttt ttgtgctgac caacctttat cgtaaggaag tgtaaaaata
     3001 agagaacctt tcgtttttga attataatca gtgattaccg tattactttt agcgtaagta
     3061 tgaacatttt tttgtttaat actattcatt gcttcaaggt aagattcaat agacaaacta
     3121 taaaaatgag gttccttaaa actaatttgt ttattttttg gaaaaacaaa cgaaaatgta
     3181 gcaactttgg catcggcgaa atatcctaaa tcaaaaaaag agtaagcgtt atctgtagta
     3241 tatatgaaat tatgattatc tgtctgaata cgcacctctt tagcatcagg atttgaaaat
     3301 ataatattgg gtatgctaac atagagttga ctatgtttag ggatgttaat ttgataatta
     3361 agggtaacgg agttttgata atcagaagct tgtgcagata tttgtccgtt aaattgatta
     3421 gcaccagaaa taagttgagc tggctgtaag ttaaaatagg ttaaagattt cccacttagt
     3481 tggttaagta atttggtttg gttatcaagg gtattgacat ttagattgac atctttgtaa
     3541 acattacgtg ttaatatagc taaagggcta ctatagtgat tttgataaag agtagtattc
     3601 ccgctagttt ttagttttgt aaatccaaat ttatttagag gtccttcgct aagattatat
     3661 tttataccaa gtagactgtc cataataata gtattgtttt ggtatctaag gtttaaattc
     3721 gtgcctttag actgaaatcc caatcgatcc aataaagaac tagatagtct atttcttaca
     3781 gaggaaaatt gtgaaatgcc gtaataatta aatttcatgc tatcgttccc tgtttgggga
     3841 agtagtcttt ccattctaaa aaaaggttga ctattttttg acacagagtt gacaaggttg
     3901 ttaatatcct ttaattgact attatatatc tgtcgtgatg ggaatcccca ctccttatta
     3961 attccttgaa gctggtagta ggtgtttaac cctgattcaa gaaggctaaa gataagtatg
     4021 aaagcagaaa taaaagtagg tgggatttga gaatttctaa acgaaaatag tgaaatagta
     4081 taacctaata ataaaaaaac actaagtaaa aaaagagtta aaggtagaaa attatattgt
     4141 tgagaaaagc tataaggaag agatgtcagt ataatgagga aaataaaagc aaaacctgct
     4201 tttatttgag tcacttcttt tagtcgagag agagtctcac atgcgagtaa taggataact
     4261 atggaaaaag accaagcgta gcgatgcaaa aacatatttg gtgagtgcat cccctgccaa
     4321 aaaagatcaa gtggctgtag gtaaaaactt attataataa aagttaacaa gcaggcattg
     4381 gctaatttta tttttaaagg gatactttct aaagtaaaat aaataacact aagcattagg
     4441 ggaaataatc ctacgtaaat cataggcaga gcattaaact tggtagtatc gtacactcct
     4501 attgagagct tagcaggtat atccaaaaac caagcattgt tcgtaactaa ctgttttacc
     4561 ggggatagat tctctccata agttgacaaa tctagatagg taggaagtat taccagagca
     4621 cttgttaaag cagcacatat tgacacagct gtaaacctaa caaaagcgat aaacattttg
     4681 ttaaaatcat tgagacttaa aagacaaact aaggcgtaaa gaatacaaaa aagagcaatc
     4741 atgtagccaa agtagtaatt ttgaatgaat aataatgata tcgaaagata ataagttctg
     4801 gttttatttt ctgtgataag tttatttaat ccaagtacaa caagtggaag aagaatgaaa
     4861 acatctaacc aagaatttag ttccatttga cttgtcaaga agctcattaa gctataaaaa
     4921 actgagatgg aaatcatcaa gaaagcattg atttttggat ataatctatg aaaagaatag
     4981 catgcagcta atcctattaa cccaaatttt atcaaggtaa acaaatagat agcatctggc
     5041 atagaggtta aattaaaaaa gaaaagtaat ggagaaaaga aactgccaag ataataacac
     5101 attaaagcat aaaaatttat ccctagtccg cttgtaaagg tataaaaaaa actatcagaa
     5161 ccgtgcatga tgttacgaaa gttctgcgca aaaataacat actgatgaaa agcatcacta
     5221 gctagaattg ttttatcact attataataa atgcccatcg atagaaggat gataaatata
     5281 atactaagag ggaacaaaaa actagcaagt ccagcaatta tccatttatt attatttttc
     5341 ataaattata tggtctttcg tttacaaatt ttaaaaaaga ggctaggaag agatcttctt
     5401 agccacctca tttatcataa ggttttattt ccaaagttca gcaactcggg cttggacttc
     5461 tggattgtca aggaattcgt cataagtttc atctatacgg tcgataacac cattttttga
     5521 aataacaacg atgtggttag caatcgtttg aataaattca tggtcatgac tggcaaagat
     5581 aacagattct ttgaagtctt tgatcccatc attaagactt gaaatagatt ccaaatccaa
     5641 gtgatttgtt ggatcatcaa gaataagaac gtttgatttc aatagcatca acttagaaag
     5701 catgacacgc actttttcgc caccggaaag aacgataaca gattttttaa cttcgtctcc
     5761 agagaaaagc atccgtccta ggaaaccacg taaaaaagta tcgtcatctt caccttttga
     5821 ggcaaattga cgtaaccatt caagaataga ttcttccgtg gcaaaatctt ttgaattgtc
     5881 tttaggcaag taagaacgac ttgttgtcac tccccattta atggttcctt cgtagtcaat
     5941 atcatctgct aaagcacgca taagagcggt tgtttgaata tcattttgac caataatagc
     6001 agctttgtca cctggacgta aaatgaaact gatattatca atgattttct ccccatcgat
     6061 cgttacagaa agattttcaa cagtgaggaa gtcattcccc atttcacgct ctgctttaaa
     6121 attaataaat gggtatttac ggcttgaggg aacaatttct tctaattcaa tcttatcaag
     6181 cattttttta cgagaggttg cttgttttga tttagatgcg tttgctgaga aacgggcaac
     6241 gaattcttgt aattctttga ttttttcttc ggctttagca ttgcggtcag cttgtaaacg
     6301 agcggctagc tcagaagatt gtttccagaa atcgtagtta cccacaaaga gtttgatttt
     6361 accaaagtca aggtcggcca tgtgagtaca tactttattc aagaagtgac ggtcatggga
     6421 aacaacgatg actgtatttt caaaatcaat caagaaatct tctaaccatg aaatagattg
     6481 aatatcaaga ccattggttg gctcgtcaag taataaaacg tcaggcttac caaagagcgc
     6541 tttagcaaga aggactttca ctttatctcc attagccagt tcactcatat tttggtagtg
     6601 aaggtcttct ggaatattta gattttggag aagttgagaa gcttcacttt ctgcctccca
     6661 gccacctaat tcagcaaaga taccttctag ctcagcggca cgaaccccat cttcttctga
     6721 aaaatctgct ttcatataga tagcgtcttt ttctttcatg atattgtaaa gttgctcatt
     6781 acccatgatt acaacatcaa tcgcacgttc ttcttcataa tcaaaatgat tttggcgaag
     6841 aacagataag cgttcgtcag gtcctaaaga gatatgacca gtacttggtt caatatctcc
     6901 tgcaaggatt ttaagaaaag ttgattttcc ggcaccattc gcaccgatta agccgtaagt
     6961 attgccggct gtgaatttga tattaacatc atcaaatagt tttcgatcac taaagcgtag
     7021 tgatacatca gaaactgtaa gcaatttgtc acctcatttt atttatctaa gcttcattgt
     7081 acctaaaata gtcccctttt tcaatcagaa aagggctaat cactacatag gcaataaaac
     7141 gtctttgtct tcacgtttgt attgtttagt caaactaaat cctcttccca taacttgagc
     7201 tgctggcatg acaacaataa aggctgtgtc gtcaatttcg aggatttttt cttggagagt
     7261 gggtaattcg tgacttgata caatagccat taacataatt ttatcagaag tagtataacc
     7321 accgcgaata ggaagtttag tcactccacg atccataaca gttgtgatat attctcgaat
     7381 agcttgataa tctttggaaa taatcataac atttttggat gagtcaaagc cattttccat
     7441 aacgctaatc acataaccaa ttacgaaaag tccaatcgta gaatacatca cgtcatctgc
     7501 agaaagtgca ataaatccca taaggacact aataccatct acaattgtca ttgcaactcc
     7561 aagagataaa ggagagtatt tatgaagtat ttgtgttaga attccagttc ctcctgtcga
     7621 agagtttccc caaaaaacca ttcctaaacc aatgccacag ataataccac caaaaatagc
     7681 tgctaacaac tgattgtgtg tcagagtagg taatgaattg gttgaacgga tggcaatagg
     7741 atatatccaa gaaccataaa gtgttttaat aaaattttgt tttccaagaa agaagtaaca
     7801 cattaataat aagggaatat tactagccat taaaaaaagt gacggactaa tgccaaatag
     7861 agcttttata acaactgcaa taccaaccat accccctgat gcaatatgat tgtcgacaaa
     7921 cattgtattg aaaccaatag ctgttatgaa tgagccaatt gtgacaagta gtaaatcaat
     7981 ccctcgtttt ttcatgcttt taatctcctt tataactggt taaaaatagt agactcatta
     8041 tctcttaaat gtactgagac cgtcaagtca tattttcaaa aaatctggtg aacttgacaa
     8101 gaagtttaaa atttcataga ataaatttga attagaagag tattcttttc atgtttttag
     8161 agagttagtg gttggtgcaa gctaacaaca gaaaaggtga aatggactaa tgattatttg
     8221 aaaggaaacc ataactttca ggttggcacc ccttaccgtg cagcttcttt cattgagaag
     8281 attgagatag tatctcgttt tgataaagca gatgctaatt gaggtggtac cgcgtattac
     8341 ttgtaataac gccctcacgt tttaatagcg tggggacttt ttgctatatc aagagatagt
     8401 tagggagaaa atgatgacaa aacctattat tttaactgga gatagaccaa ctggaaaatt
     8461 acatttaggt cattatgtcg gaagtcttaa aaatcgtgtc tttttacaaa atgaaaacaa
     8521 gtataagatg tttgttttct tggccgatca acaggcactg acggatcatg ctaaagaatc
     8581 cgaattaatt caagaatcta ttggaaatgt tgccttggat tacctctctg tcggtttgga
     8641 tccgaagcaa tcaacgattt ttattcaaag tcagattcca gagctagctg aattgagcat
     8701 gtattatatg aatctggtat cattagcacg tttggaaaga aatcccactg ttaaaaccga
     8761 aattgctcag aaaggttttg gcgaaagtat tccatctggt tttttggttt atccagtatc
     8821 acaggccgct gatattacag catttaaagc gaatttagta cctgtaggta acgatcagaa
     8881 accgatgatt gaacaaacac gtgaaattgt gagaagtttt aatcatactt accatacaga
     8941 ctgtttagta gaacctgaag gtatttatcc agaaaatgaa aaagctggac gcttacctgg
     9001 tcttgatggc aatgccaaaa tgtctaaatc attgggaaat ggaatctatc tttcagatga
     9061 tgcagatacc gttcgcaaaa aagtgatgag catgtatact gatccaaatc atattaaaat
     9121 agaagatcct ggtcaaattg aagggaatat ggtctttcat tatttggata tttttgctag
     9181 aaaagaagat caagctgata tcgaagcaat gaaagagcat tatcaaagag gtggtttagg
     9241 agatgtgaaa acgaaacgct accttctaga tattttagaa cgtgaattag cacctattcg
     9301 tgaaagacgt ttggagtacg ctaaagatat gggagaggtg ttccgtatgt tacaagaagg
     9361 tagtcaaaaa gcaagaactg tggcagccaa gactttatca gaagtgaagt cagcaatggg
     9421 tattaattat ttttaatgtt ttagagaaaa agctaagtta gtaatgataa gataattact
     9481 attatcaaag caggacctaa tttgtcattt ctttacagga tagtagagat tatgtttta
//
LOCUS       DAUVUY010000071         9533 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.071, whole genome shotgun sequence.
ACCESSION   DAUVUY010000071 DAUVUY010000000
VERSION     DAUVUY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.071"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            67..372
                     /gene="rplX"
                     /locus_tag="VOX58_001466"
     CDS             67..372
                     /gene="rplX"
                     /locus_tag="VOX58_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HES5878686.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            396..938
                     /gene="rplE"
                     /locus_tag="VOX58_001467"
     CDS             396..938
                     /gene="rplE"
                     /locus_tag="VOX58_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HES5878687.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            954..1139
                     /locus_tag="VOX58_001468"
     CDS             954..1139
                     /locus_tag="VOX58_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HES5878688.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            1290..1688
                     /gene="rpsH"
                     /locus_tag="VOX58_001469"
     CDS             1290..1688
                     /gene="rpsH"
                     /locus_tag="VOX58_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HES5878689.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            1891..2427
                     /gene="rplF"
                     /locus_tag="VOX58_001470"
     CDS             1891..2427
                     /gene="rplF"
                     /locus_tag="VOX58_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HES5878690.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            2532..2888
                     /gene="rplR"
                     /locus_tag="VOX58_001471"
     CDS             2532..2888
                     /gene="rplR"
                     /locus_tag="VOX58_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HES5878691.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            2907..3401
                     /gene="rpsE"
                     /locus_tag="VOX58_001472"
     CDS             2907..3401
                     /gene="rpsE"
                     /locus_tag="VOX58_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HES5878692.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            3416..3598
                     /gene="rpmD"
                     /locus_tag="VOX58_001473"
     CDS             3416..3598
                     /gene="rpmD"
                     /locus_tag="VOX58_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HES5878693.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            3813..4253
                     /gene="rplO"
                     /locus_tag="VOX58_001474"
     CDS             3813..4253
                     /gene="rplO"
                     /locus_tag="VOX58_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HES5878694.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            4270..5574
                     /gene="secY"
                     /locus_tag="VOX58_001475"
     CDS             4270..5574
                     /gene="secY"
                     /locus_tag="VOX58_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HES5878695.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            5724..6362
                     /locus_tag="VOX58_001476"
     CDS             5724..6362
                     /locus_tag="VOX58_001476"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HES5878696.1"
                     /translation="MNLLIIGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            6480..6698
                     /gene="infA"
                     /locus_tag="VOX58_001477"
     CDS             6480..6698
                     /gene="infA"
                     /locus_tag="VOX58_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HES5878697.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            6724..6840
                     /gene="rpmJ"
                     /locus_tag="VOX58_001478"
     CDS             6724..6840
                     /gene="rpmJ"
                     /locus_tag="VOX58_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HES5878698.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            6858..7223
                     /gene="rpsM"
                     /locus_tag="VOX58_001479"
     CDS             6858..7223
                     /gene="rpsM"
                     /locus_tag="VOX58_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HES5878699.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            7241..7624
                     /gene="rpsK"
                     /locus_tag="VOX58_001480"
     CDS             7241..7624
                     /gene="rpsK"
                     /locus_tag="VOX58_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HES5878700.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            7670..8608
                     /locus_tag="VOX58_001481"
     CDS             7670..8608
                     /locus_tag="VOX58_001481"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HES5878701.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDERVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            8623..9009
                     /gene="rplQ"
                     /locus_tag="VOX58_001482"
     CDS             8623..9009
                     /gene="rplQ"
                     /locus_tag="VOX58_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HES5878702.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
BASE COUNT         3126 a         1675 c         1979 g         2753 t
ORIGIN      
        1 aaacgtagtc ccctaggttt tcctagggtg cccatcgggg cgtaagaaat aataggagaa
       61 accagaatgt ttgtaaaaaa aggcgacaaa gttcgcgtaa ttgctggcaa ggacaaaggc
      121 actgaagctg tagttcttaa agcactccca aaagttaaca aagttattgt tgaaggtgtt
      181 ggaatgatta aaaaacacca aaaacctaac actgaaaatc ctcaaggagc tatcgtcgaa
      241 aaagaagcac ctatccatgt ctctaatgtt caagtattag ataaaaatgg tgtagctgga
      301 cgtgttggtt ataaagttgt tgacggtaaa aaagtacgtt acagcaaaaa atcaggcgaa
      361 gtgcttgatt aatcacgaag gaaaggagaa gcataatggc aaatcgttta aaagaaaaat
      421 atactaacga agtaattcct gcgttgacag agaaattcaa ttacacatca gttatggctg
      481 tgccaaaagt tgagaaaatt gttctcaata tgggtgttgg tgatgctgta tccaacgcaa
      541 aaaacctaga gaaagctgca gctgaattag cacttatctc aggtcaaaaa ccacttatta
      601 ctaaagctaa gaaatcaatc gctggcttcc gtcttcgtga aggtgtagcg atcggtgcaa
      661 aagttaccct ccgtggcgaa cgtatgtacg aattcctaga caaattggtt agcgtttcac
      721 ttcctcgtgt acgtgacttc catggcgttc cgacaaaatc gtttgatgga cgtggaaact
      781 acactctcgg tgtgaaagaa caacttatct tcccagaaat cagctttgat gatgttgata
      841 aagtacgtgg tcttgatatt gtaatcgtca ctactgcaaa tactgacgaa gaatcacgtg
      901 aattgcttaa gggccttgga atgccttttg caaaataata gggggtaaat aaattggcta
      961 aaaaatctat gattgctaag aacaaacgtc ctgcaaaaca ctctacacaa gcttatactc
     1021 gctgtgaaaa atgtggacgt ccacattcag tttaccgcaa gtttaaactt tgtcgtgttt
     1081 gcttccgtga attggcttac aaaggtcaaa ttccaggtgt tgtcaaagct tcttggtaaa
     1141 aatatgacac aaaacttaat ttgttcttca gattaagtct tgtgcttaat taaaacggtg
     1201 tgagcataag ctgataccaa agtcctttaa ggacaacatt aactagtaag tgatagcatc
     1261 aaactgctag taagaggaga atataaaaaa tggttatgac tgacccaatt gcagactttt
     1321 taacacgtat ccgtaatgct aatcaagtaa aacacgaagt gttagaagta cctgcttcaa
     1381 acattaaaaa agggattgct gagattctta aacgtgaagg ttttgtaaaa aatgttgaag
     1441 ttatcgaaga tgataaacaa ggcatcattc gtgtgttctt aaaatacgga aaaaatggtg
     1501 agcgtgttat cactaactta aaacgtattt caaaaccagg tcttcgcgtt tacgcaaaac
     1561 gtgatgacat gcctaaagtt cttaacggac ttggaattgc aatcatttca acatcagaag
     1621 gtcttttgac tgacaaagaa gctcgccaaa agaatgttgg tggagaagtg atcgcttacg
     1681 tttggtaatt taagatacta agagcgtcgt ggacaaagtg aaaataggaa atctgacgta
     1741 gcgtgtttac actcaaggaa gatttatctt tttcacacag accacagctc gtgttcaatt
     1801 ttgaaatata aattgaagta tatcattcta tcaaaccccc gtgaaaacta gtcgctatgc
     1861 ggcttgacaa tttaacagga gaaaataaac atgtcacgta ttggtaataa agtaattact
     1921 atgcctgcag gcgttgaatt aacaaataac aacaatgtta ttactgttaa aggccctaaa
     1981 ggcgaactca ctcgtgagtt caacaaaaat attgaaatca aagttgaagg gactgaaatc
     2041 acagttgtac gtcctaacga ctcaaaagaa atgaaaacaa tccatgggac aacccgtgct
     2101 aacttgaata acatggttgt aggtgtttct gaaggtttca aaaaagatct tgaaatgaag
     2161 ggtgtcggtt accgtgctca acttcaaggt actaaacttg tcctttcagt aggtaagtct
     2221 caccaagacg aagttgaagc tccagaagga attactttca ctgttgctaa cccaacttca
     2281 atctcagttg aaggaatcaa caaagaagtt gttggtcaaa cagctgctta catccgtagc
     2341 ttgcgttcac cagagcctta caaaggcaaa gggatccgtt acgttggtga atacgtacgc
     2401 cttaaagaag gtaaaacagg taaataatgt tcttagtcgg gtatgggaag tattgcacct
     2461 taacatgctc gataagttca atcgacaact tgtcaatgag acaaattgat taaaattaag
     2521 aggtgaaaat tgtgatttcg aaaccagata aaaacaaaat ccgccaaaaa cgccaccgtc
     2581 gcgtccgcgg taaactctct ggaactgctg atcgcccacg tttgaacgta ttccgttcta
     2641 atacaggcat ctacgctcaa gttattgatg acgtagcggg tgtaacgctc gcaagtgcat
     2701 caactcttga taaagacgtt tctaaaggaa caaaaacaga acaagccgtt gtagtcggca
     2761 aacttgttgc tgaacgtgca gtggctaaag gtatttctga agtggtgttt gaccgcggtg
     2821 gatatctcta tcacggacgt gttaaagcct tggctgatgc agctcgtgaa aacggattga
     2881 aattctaata gggaggacac aaaataatgg catttaaaga taatgcagtt gaacttgaag
     2941 aacgcgttgt tgcgattaac cgcgttacaa aagttgttaa aggtggacgt cgtcttcgct
     3001 ttgcagctct tgtagttgtt ggtgatggaa atggtcgtgt tggatttggt actggtaaag
     3061 ctcaagaagt tccagaagct atccgtaaag cagttgaagc tgctaagaaa aacatgatcg
     3121 aagtaccaat ggttggtaca acaatccctc acgaagttta cactaacttc ggtggagcta
     3181 aagtattatt gaaaccagct gtagaaggtt ctggagttgc tgctggtggt gcagttcgtg
     3241 ccgtcatcga attagcaggt gttgctgata ttacttcaaa atctcttggt tcaaacactc
     3301 caatcaacat tgttcgtgca actgttgaag gattgaaaca acttaaacgt gcagaagaag
     3361 ttgccgcact tcgtggcatc tcagtttctg acttagcata agaaagggga tcatcatggc
     3421 tcaaattaaa attactttga ctaagtctcc tatcggacgt aagccagaac aacgtaaaac
     3481 tgttgttgct cttggacttg gtaaattaaa ctcttcagta gtcaaagaag ataatgctgc
     3541 tatccgtggt atggtgacag ctatttctca cttggttacc gttgaagatg ttaaataagt
     3601 tataactgtg aaatctctga tttcataatt aaaaacgagc acaaaaccga ctcttgtagt
     3661 ttgctttttt ggcaacaaac agtagtcggt tagtgcgtat ctgtattaag ttattttgga
     3721 taacttaagt atcattaatg tataggcgag tttctatagg gagtcctttt ccctcataga
     3781 ggcgctagca ttttacaaaa aaaggagaaa aaatgaaact tcatgaatta aaagctgctg
     3841 aaggctcacg taaagtacgt aaccgtgttg gtcgtgggac atcatcaggt aatggtaaaa
     3901 catctggtcg cggtcaaaaa ggtcaaaaag ctcgtagcgg tggcggtgta cgtttaggtt
     3961 ttgaaggtgg acaaacacca ttgttccgtc gtattccaaa acgtggcttt acaaatatta
     4021 atactaaaga atatgcactt gtgaatcttg atcaactgaa tgtttttgat gatggtacag
     4081 aagtgactcc ggctatttta aaagatgctg gtattgtccg agctgaaaaa tcaggcgtta
     4141 aagtgcttgg taacggtgaa ttgactaaaa aattgactgt aaaagcagct aagttctcaa
     4201 aatctgctga agcagctatt attgctaaag gtggttcaat cgaagtcatc taatgagggg
     4261 atactcatta tgttcttaaa aatactaaaa gacgcgttaa agataaagac tgtacggaat
     4321 aaaattttct ttactatatt tatcattctt gtattccgaa ttgggacaca tattaccgta
     4381 cctggtgtaa atgccaagag cttagagcaa ttaagtgaac tccctttctt aaatatgtta
     4441 aacttagtta gtggtaatgc gatgagaaat ttctcagtat tttcaatggg ggttagccca
     4501 tatattactg cttctatcgt tgttcagtta ttgcagatgg atattttacc taagtttgtt
     4561 gagtggggca aacaaggtga ggttggacgt cgtaaattaa atcaagcgac gcgctatatt
     4621 tcacttgttt tggcttttgc ccaatccatt ggtatcactg cagggtttaa tactttgtcg
     4681 aacgttgcac ttgttaagac accagacatc aaaacatatt tactgatcgg ggcattactg
     4741 acaacaggta gcgttattgt tacctggctt ggagaacaaa tcaccgataa aggatttggt
     4801 aatggcgtat caatgattat ctttgcgggt attatttcgt ctattccaag tgcaattgca
     4861 acaattcgtg aagactattt tgtaaatgtt aaggcaagtg atttacactc gtcttatctc
     4921 attgttggga ttttaatcat agctgttctt gctattgttt tctttacaac atatgtccaa
     4981 caagcggaat ataaaattcc aatccaatac acaaagctaa tgcaaggtgc acctacaagt
     5041 tcatatcttc cattaaaagt aaatccagcc ggcgttattc ccgttatctt tgctagctcg
     5101 attacaacta tcccaagtac gattattcct tttgtccaaa atggtagaga tttaccgtgg
     5161 ttaaaccgtt tgcaagaaat ttttaattat caaactccag tcggaatgat agtttacgcc
     5221 ttgttgatta tattgttctc attcttctat acctttgtac aagttaatcc tgagaagaca
     5281 gcagaaaatc ttcagaagaa ttcctcatat ataccaagtg ttcgccctgg acgtgagaca
     5341 gaacaattta tgtccgcatt gctaaaaaaa ctagcaaccg taggagctat cttcttagca
     5401 tttatctctt tagcgccaat agcagcacaa caagctctca acctttcttc tagtattgct
     5461 ttaggtggaa ctagtttgct cattttgatt tcaactggta tcgaaggtat gaaacagctt
     5521 gaggggtatc ttctaaagag aaaatatgtc ggatttatga atacagcaga atagaataca
     5581 ggttgacatt gtcaaccttc tattttgttt ttaaatgaca ataagacttg gttatcatca
     5641 aacgatttct tgttgccatt tgaaaacaaa aaccaaggaa aataatttcc tactatgtat
     5701 ttattttaaa agaggaggaa aagatgaatc ttttaatcat aggtttgccg ggagctggta
     5761 aagggaccca agcagctaag attgttgaag aatttggtgt tgctcacatc tcaacagggg
     5821 atatgttccg cgccgcaatg gctaatcaaa ccgaaatggg acgtttagct aaaagttata
     5881 ttgataaagg tgaattggtt cctgatgaag tcacaaacgg gattgtaaaa gagcgcttag
     5941 ctgaggatga tatcgcagaa aaaggttttt tgcttgatgg gtatccacgt actattgaac
     6001 aagcacacgc cttagatgca acacttgaag aactaggact ccgcttagat ggtgttatta
     6061 atattaaagt ggatccatca tgtcttgtag agcgtttgag tggtcgtatt atcaatcgta
     6121 aaactggtga aacgttccat aaagtgttca acccaccagt agattataaa gaagaagatt
     6181 actatcaacg tgaagatgat aagcctgaaa ctgttaaacg tcgtttggac gtcaatatgg
     6241 ctcaaggaga acctattctt gaacactatc gtaagcttgg tcttgttaca gatattgaag
     6301 gtaatcaaga aataacagac gtttttgcag atgttgaaaa agcgttgcta gaactcaaat
     6361 aaattatact aatctacttg cataagtaga ttagtaatga tatactagtt tagtctgact
     6421 tataattgtt acctctgtgc tcagaggaca tcaaatcgaa atttaggggg tacttttgcg
     6481 tggcaaaaga agacgtgatt gaaattgaag gcaaagtagt agaaacgatg ccaaatgcaa
     6541 tgtttactgt tgaattggaa aatggacatc aaattctagc aactgtatca ggaaaaatcc
     6601 gcaaaaatta cattcgtatt ttagtaggtg accgtgttac agtagaaatg agtccttatg
     6661 acttgacacg tggacgcatc acataccgct ttaaataatc gaaataattg gagggattaa
     6721 aacatgaagg taagaccatc ggttaaacca atttgcgaat actgtaaagt tattcgccgt
     6781 aatggtcgtg ttatggtaat ttgtccgaca aatccaaaac acaaacaacg tcaaggataa
     6841 aatagaaagg agaaaaaatg gctcgtattg ctggagttga tattccaaat gataaacgcg
     6901 tagtaatttc acttacttat gtttacggaa ttggtcttgc aacatctaaa aaaatcttag
     6961 cagctgcagg tatttctgaa gatatccgtg ttaaagattt aacatcagat caagaagatg
     7021 ctatccgtcg tgaagtggat gcaatcaaag ttgaaggtga ccttcgacgt gaagtaaaca
     7081 tgaacattaa acgtttgatg gaaatcggtt cataccgtgg aatccgtcat cgtcgtggac
     7141 ttcctgtccg tggacaaaat actaaaaaca atgctcgcac tcgtaaaggg aaagctgttg
     7201 cgattgcagg taagaaaaaa taaaatagga ggtaaaacaa ttggctaaac caacacgtaa
     7261 acgtcgtgtg aaaaagaaca tcgaatctgg tgttgcacac attcacgcta catttaataa
     7321 cactattgtt atgattacag atgtgcatgg taatgctctt gcatggtcat cagctggtgc
     7381 tcttggtttc aaaggttctc gtaaatctac tccatttgct gctcaaatgg ctgcagaagc
     7441 tgctgcaaaa tctgcacaag aacacggact aaaaactgtt gaagtgactg taaaaggtcc
     7501 tggttcaggt cgtgaatcag ctattcgtgc tctagcagct gcaggtcttg aagtaactgc
     7561 aattcgtgat gtgacccctg tgccacataa tggtgctcgt cctccaaaac gtcgtcgtgt
     7621 ataatcatat aacaaatagt acacaagttt cgtttcgagg ggtgaaaaaa tgattgagtt
     7681 tgaaaaacca ataataacaa aaattgatga aaataaagat tacggtagat ttgtcatcga
     7741 accacttgaa cgtggctacg gaacaactct aggtaattct cttcgtcgtg tactcttgtc
     7801 ttcacttcca ggtgcagcag taacatcaat taaaattgat ggagtactac acgaatttga
     7861 tacaatccca ggtgtacgtg aagatgtcat gcaaattatc cttaatgtta agggacttgc
     7921 tgtgaaatca tacgtcgaag acgaaaagat tatcgaactt gaagtagaag gaccagcaga
     7981 ggtaacagct ggtgatattc ttactgacag tgatatcgaa cttgttaacc cggatcatta
     8041 tctttttaca atcgctgaag gacattctct acgtgcaaca atgactgttg ctaaaaaacg
     8101 tggttatgtg ccagcagaag gaaacaaaaa agatgatgca cctgtgggta cattggcagt
     8161 agattcaatc tatacgccag ttaaaaaagt taattatcaa gttgaacctg cccgtgtagg
     8221 tagcaatgat ggctttgata aattaacgat tgaaatcatg acaaacggaa caattattcc
     8281 tgaagatgct ttaggtctat ctgctcgagt tttgatcgaa cacttaaacc tgtttactga
     8341 tttaacagag gttgcaaaag ctaccgaagt aatgaaagaa actgaaaaag tgaacgatga
     8401 aagggtactt gaccgcacca tcgaggagct tgatttatcc gtacgctcat ataactgttt
     8461 aaaacgtgca ggcattaaca ctgtcttcga cttaacagaa aaatctgagc ctgaaatgat
     8521 gaaagtccgt aatcttggac gtaagagcct tgaagaagtt aaggttaaac ttgctgactt
     8581 aggtctcgga ctaaaaaacg ataaataata taggaggaca aaatggctta ccgtaaacta
     8641 ggacgcacta gctcacaacg taaagcaatg cttcgtgatt tgacgacaga tcttttgatc
     8701 aacgaatcaa ttgtaacgac agaagcacgt gcaaaagaaa tccgtaaaac agttgaaaaa
     8761 atgattactc ttggtaaacg tggtgatctt catgctcgtc gccaagcagc tgcatacgtt
     8821 cgtaacgaaa tcgcatcaga aaattatgat gaagctaccg ataaatacac atcaacgaca
     8881 gctcttcaaa aacttttctc agaaattgca cctcgttatg cagaacgtaa cggtggatac
     8941 actcgtattc ttaaaacaga accacgccgt ggagatgctg ctccaatggc aattattgaa
     9001 ttagtttaat ttttatcaat tttgttgagt gttatgatga tggagtaatc gagagattat
     9061 tcttagtcta gctctggtct accgctagga tttttcctag cgggaacact catcatattg
     9121 ttaatatgtg acgcttgttt atgaaaggta tctaagctat agaaactttt tataagcagg
     9181 cgttttgttg tattatgagg tgacactccc gcttttaagc tgagtaaaac aataacacag
     9241 aagcctcttc tgatgcgttt ataaagtttt ctcagagaca ataaggtcat cagaataaga
     9301 aaaccttatg caggtgttta aagacctctc atgggcaaat aaacggtgta atcccctcgt
     9361 ttaaatgtcc gaaaaaaact tgcatcgcaa acagatgctc aaactatatt gttagtttta
     9421 tcccctattc aatattttat taataagtat tttaaaatcc tcttttaatt tggaggtgaa
     9481 aatatctttt ttacttttac aatctgtcac tttaagccgt ttccaacaaa att
//
LOCUS       DAUVUY010000072         9509 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.072, whole genome shotgun sequence.
ACCESSION   DAUVUY010000072 DAUVUY010000000
VERSION     DAUVUY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.072"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..676
                     /locus_tag="VOX58_001483"
     CDS             <1..676
                     /locus_tag="VOX58_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878703.1"
                     /translation="QQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKR
                     TLYNLQTLITFDGYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYL
                     EQILESSIAPINITVIESQETVGNVIGNFITSTIPSYKVELSRVNILSDNIYPYDKPV
                     DLVVTSQKLLPFLKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEE
                     LWRVPS"
     gene            complement(1025..2374)
                     /locus_tag="VOX58_001484"
     CDS             complement(1025..2374)
                     /locus_tag="VOX58_001484"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HES5878704.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKLEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
     gene            complement(2830..3534)
                     /gene="speG"
                     /locus_tag="VOX58_001485"
     CDS             complement(2830..3534)
                     /gene="speG"
                     /locus_tag="VOX58_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
                     /protein_id="HES5878705.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEIAFVTTNSIHINTKQKRSECILYVDSIVSLGITDQFIKGDKVDVFGLPYNFS
                     PPYVDNIYGGIVKHSNQGNKSLQFVGILNQDGKETYLPSEAVRIKKKQFTLQEFDFKI
                     RKFLMEKYNIYDSESRYTSGSLFLATKDSKHYEVDLFNKDDKLLSRDSFFKRYKDNKI
                     FNSEEISHFDIYLKTH"
     gene            complement(3636..3734)
                     /gene="ffs"
                     /locus_tag="VOX58_001486"
     ncRNA           complement(3636..3734)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VOX58_001486"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3934..5166
                     /locus_tag="VOX58_001487"
     CDS             3934..5166
                     /locus_tag="VOX58_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HES5878706.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQAQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEQLCLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(5780..6295)
                     /locus_tag="VOX58_001488"
     CDS             complement(5780..6295)
                     /locus_tag="VOX58_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HES5878707.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(6295..7074)
                     /locus_tag="VOX58_001489"
     CDS             complement(6295..7074)
                     /locus_tag="VOX58_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HES5878708.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PEGIDPDNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(7217..7753)
                     /locus_tag="VOX58_001490"
     CDS             complement(7217..7753)
                     /locus_tag="VOX58_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HES5878709.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTK"
     gene            complement(7757..8068)
                     /locus_tag="VOX58_001491"
     CDS             complement(7757..8068)
                     /locus_tag="VOX58_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878710.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(8285..9427)
                     /gene="tgt"
                     /locus_tag="VOX58_001492"
     CDS             complement(8285..9427)
                     /gene="tgt"
                     /locus_tag="VOX58_001492"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HES5878711.1"
                     /translation="MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVIKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
BASE COUNT         2947 a         1967 c         1707 g         2888 t
ORIGIN      
        1 gcaacaacaa ctttaccatt taatcaccaa agatcccgat tatcatttac taatccatcg
       61 tcttcaggca ctagttggaa aatattccga tattcatgag catatcccta acttaattcc
      121 ttttttcaaa agaactctct acaatttaca aactttaatt acctttgatg gttattattt
      181 tgatcaatat caaggcaaca tgctactact agataagctt gaaaccgtta tcaaagattg
      241 gctttatgac acaggccgtc aaggcagtat tagttctggt cacttacact tgatgtgttt
      301 ataccttgag cagatcttgg aaagctctat tgctccaatc aatattactg ttattgagag
      361 ccaagaaact gttggtaacg ttataggtaa tttcatcacc tcaactatcc cttcttacaa
      421 agttgaactg tcacgggtca atatcttatc tgataatatt tatccctatg ataaacctgt
      481 agatctggtt gtgaccagtc agaagctctt gcctttctta aaagaactcg gtgtttttcc
      541 caaagagacc cgcttgtttg gtttgagttt ggactgtatc caacagcagc gcgaagattt
      601 aatcaaaacc atcttagcct tgcaccagaa ccactaccaa aaacgtttag aagaactttg
      661 gagagttcct tcttaatttt ttgacagtcc ctcctagata cttttacagt ctaggggggt
      721 ctttaaggtt gtcacttgca accttgataa gctctctttt taaccattgc tatttgctta
      781 gaaacagaac tggaactgtt ttagcggttc aactcctctt gtaggatctt cttccctttt
      841 ttgaaggttt tatctgaaat ttggcataat aaagtgcgct cccatgttct ggaagcgcac
      901 tttattatcg cttttactta accttgtatc cacttgatga tggaagtgcc gccccaattc
      961 ttagattgct tggctaactt ttaagctctt gtacaaggaa gaagtctttt tgactatctg
     1021 ttgattagag gcgagcatta agctctgctg ataatgcttc aaaacctggt tttccaagaa
     1081 gcgcaaacat attacgtttg taagcttcaa cacctggttg atcaaatggg ttaacagcat
     1141 tcatgtagcc tgaaacagcg atggcaagct caaagaagta aatagtgtaa ccaagggtaa
     1201 actcgtcttg agctggaaga gtgacaaaca tgtttggcac gccaccatct gtgtgggcaa
     1261 gaagcacacc atctgtggct ttcttattaa caaaatcaac gtctttacct tgaaggtaac
     1321 caagaccatc taggtcttct gctaattcag gaatgataac atttttacga gggttatcaa
     1381 cacgaataac tgtttcaaag aggttacggt aaccttcttg gataaattga ccaagtgagt
     1441 gaaggtctgt tgagaagtta gctgaagttg ggtaaatccc tttttgatct tttccttctg
     1501 attcaccagc taattgtttc caccattcgc caaagtactg aagagatggc tcgtagttag
     1561 ccaagatttc agtgatgtag cctttacggt aaagcacatt acgaacagca gcgtattggt
     1621 aagcaatgtt ttcagagatt ttgtctgaag aaaggtcttt acgagcagca tttgcccctt
     1681 ccataagagc agtaatatct gcacctgatg cagcaattgg aagcaaacca acagcagtca
     1741 agactgagaa acgaccacca acattatctg gcaccacaaa tgtttcccag ttgttagcgt
     1801 cagcttctaa cttaacagca cccttaactt tgtctgtggt agcgtagata cgtttgttag
     1861 cttcttcttg gccatatttt ttaaccaaaa gttctttgaa cacgcgaaaa gcaatagctg
     1921 gctcagtggt tgtacctgat ttagagatca cgtttactga gaattctttg tcttgaacgt
     1981 actctacaag gtcagctaag taagttgatg aaatagagtt tccagcgtaa agaatttgtg
     2041 gggctttgcg ctctttggca gtttgcaagt tagcaaaatg atgatttaaa aaatcaatcg
     2101 ctgctttggc accaaggtat gacccaccga taccgatcac cacaagaact tcgctgtctg
     2161 ccttaatctt ttcagcagca gtcaagatac gtgcaaattc ctctttgtca tagttttctg
     2221 gaagatcaag ccagcccaag aaatctgaac ctggtccagt tccttcacgc aataacttgt
     2281 cagcttctgt gacttggcct tgtaaaaaat caatttcatg ttgtcccgca aatgactcga
     2341 ggacttttga ataatcaaat gtaatatgtg acatgagttt cgctccctta aaattttaag
     2401 tttcattagt atgataacct tttctatcta atatttcaat ggtttttagt ggttttcacc
     2461 tgtgaaaaag ctaggttttt tgaaaaggtt ttcgtttttt cagacataaa aaatgtgtca
     2521 atgcaagcat attctcatca tagtggtttt ttaagagaaa ataggatttc atatttccta
     2581 aaaatatcat gaaaaaaggc cctgaggccg ctttaccgta ataacttatt ttaaggattt
     2641 tatcatttca acacgggtaa taaccccatc atgcatatga tcaatgcata aagccacacc
     2701 tacaaaggca attgaagccc caatcacctg taaatacttt ctcatgctat cttcttaaca
     2761 ttacttactc tcattatcta aaaatggcaa tcatttgaat acttaaaaag caaagggggg
     2821 agaatagcac tagtgcgttt ttaagtagat atcaaaatga ctaatttctt cactattaaa
     2881 aatcttatta tctttatacc ttttaaagaa actgtctcga cttaaaagct tatcatcctt
     2941 attaaataaa tcaacttcat aatgtttact atctttagta gcaaggaaaa ggctccccga
     3001 tgtataacgc gattccgaat catagatatt gtatttttcc attagaaatt ttcttatttt
     3061 aaaatcaaat tcctgtaaag taaactgttt cttttttatg cgaacagcct cagagggcaa
     3121 ataagtttct ttcccatctt gatttaaaat tcctacaaac tgtaatgatt tatttccttg
     3181 attcgaatgt tttacaatac caccataaat attatctaca taaggtgggg aaaaattata
     3241 agggagacca aaaacatcga ccttatcccc ttttataaac tgatcagtaa tgcctaaaga
     3301 tacaatagaa tcaacataaa gaatacattc cgatcttttt tgtttagtat taatatgtat
     3361 gctatttgta gtaacaaatg caatttctac attttcataa tcgcatgggg taatattata
     3421 ggcaaacctt aaacttcttt ttaaatcttt taaattttca tctgcataag ctaattgact
     3481 tccatagcta aaaacacatg ataatatgat aattgtcaaa atgtttgttt tcatttaaaa
     3541 ctttttatgt cctgttttat acctcttaac ctaaataaaa atatcaatcg gtttcaacaa
     3601 ctgatgacac tatagaagga ggagattact ttagtaaagt caacaaaaaa agagcacaca
     3661 cccgaaaatc gcttagggct gctggattcc tcccctgacc cacttcacgc acaagtgttg
     3721 ctccgcctat tattatatca tattacggac agtttgcaag ttttccaact aagtaaaatc
     3781 actttcaagt gagcatagct tcattttggg acaagatgga ctgaatacct agaaaaatca
     3841 tgaccctttc caaacacgat tttgcttttt gaaaatcttt ccctctcccc attccagcta
     3901 ttctctatgt tataataaga ttattagatt agaatgggag ttatgatgaa acaaaagatt
     3961 aaaatcttaa cagtaattgg ccttatgaca gttggtatgt ctgcgtgtca caacactagc
     4021 aaaccttcta ataccgattc tgttttttct ttaaccggaa aaaagcgcca acaaatcgtc
     4081 aaacaagtaa gacaacggta ttatttccaa caattatcca aaaccgaaca agaaaattac
     4141 ctcactttat acgatagtct agcacaattt cgtgaaatca tcagtttgac gccggcttct
     4201 aaaaaaagct taattaaaac gattgacgcc tttgtgatgg ataatcccga attttactgg
     4261 attacatctg ccgattatcg atttgagttt tctgatcaaa cggtctttgt cacctttcct
     4321 atcccagaag atgccaagaa cgtttaccaa gacttgcaag caatcggtaa tgatatcgtc
     4381 gcaaacacgc catcaaagga tcgctatgag caggtcaaat atttttatga ggtcatcatt
     4441 cgagacacag actacaataa aaaagccttt gaagcctatc aatcaggcag tcaagcccag
     4501 gtcgcttcta atcaagacat taaaagtgtt tttattgatc atttatctgt ttgtaatggc
     4561 tatgcccaag cctttcagtt tctttgtcaa aaagctggca ttccagtcgc ctatatccgt
     4621 ggaactggta catctcagca acctcagcaa tcttttgcac atgcatggaa tgctgttcaa
     4681 attaataaca cttattatgg cgttgatgtc acttggggtg accctgtttt tgataaccat
     4741 ctctcacatc aaaagcaagg aactatcaat tacagttttc tatgcctgcc agattatcta
     4801 atggccttat cacatcagcc aagcaaagac attgctttta atactaaaga acgttttgaa
     4861 aatgtttgga ctataccttc ttgtacagat gattctttgc tttattctaa acgtcatcaa
     4921 agctatatct caacgtttga cagtgacgcc atccttgcaa gccttgaaaa tcagttgtta
     4981 aatagacaag agcaactatg tctacaattt gctcatcaag atgattatca acagatggta
     5041 actgatttaa cgacgaatca aacaggttat cataaccttt tcaatcagta ttggaataat
     5101 tatacgggct ttacctatgg actcttacca gagactctta gtatttcatt tgctagtagg
     5161 aactaggttt agacacatga aacacaaagt taagatgatt atctttgtca aacattacaa
     5221 gaaattgata tactgacaca attgaaggcg attattattc taaccgtctt caatagatat
     5281 aaccttttca gattgagcac atctaatgct tgtaacatta gcaacagtga tgagctcttt
     5341 tttaatgctt tagcaaaata tatcagctaa gacaaaagat taaataactc aagccatgcc
     5401 tataagtcac ttgtttttaa tccctgtgtc agtgtcaatc tacgccacag ataactgaca
     5461 gctctgagcc ttagaagtct gagctggata agtttactaa aaacaaaaat aaggtgagcc
     5521 attactatct gtttttagat agtaaatctt tttaagaatg actaactcat ctagtcatca
     5581 cgtgttttat ttttcgattg ttttcacctc gattgggtct gaaaaagagc gcttctagta
     5641 cttggaaacg cccttttgta tattctatct tcgtatgttg aatctaacaa ccattggaaa
     5701 aatggtctaa cgtttcactc attattttga caggttaccc ttcaccaaaa atgcatgtga
     5761 taacaagctc cttaaaactc taatcaaagg gatctgactg ttccttgatc aaatgcttag
     5821 ctattttttt gcgctctcgc ccttggcgga aaaaggtttg catgatatta gcacagtctg
     5881 ctgctagcag gcctcgttca acttgtacgc ggtggtttaa gcgctcatct gtcaaaatct
     5941 ggtaaaggct gtctgctcca ccaaattttt gattgctggc tccataaata acatgcggaa
     6001 tccttgctag cccaatggca ccactacaca tcacacaagg ctcgatggtc acaaagagtg
     6061 tggtgtctaa taaacgccag ttgccttcat gagcgttagc ctcgttaatg gccatcattt
     6121 cagcgtgcat aatggcttgg ttactctctt cacgcgcatt gtgcccacga ccaataatct
     6181 caccatcctt gacaatgaca cagccaatgg ggatttcagc tttctgtaag gatttttcgg
     6241 cttcttttaa agcctcttgc atgaaataag tttgctcttc taaactatat ggcatcaaga
     6301 aagactcccc aagttccaag atttgtgtgg taaggcaagg tcaacaggaa tattacctgc
     6361 ataggcttgt gtttcttgcc tcaacacttc caagtgattg tcaggatcaa taccttctgg
     6421 aggaaccggt ggcatgtggg tcaagacaag cttcttaaca cctgcctggc tagccattag
     6481 tcctgcttcc ttactggtca aatgggccat atgattttca ttgccttcgt ataagtaaac
     6541 atctgctaag aaaaggtctg cttcttttgc gaagtctgcc aatccttcaa aataaccact
     6601 gtctccagta aagacaagta cctgaccagt cgctctttct acaattcgaa aggcataaca
     6661 cacaactgga tgaactgttt taagaaaggt aatgtcaaaa ggaccaatct gttcaactcc
     6721 agtgacatca taggctctac cttctgaaac atcaggaatg cttaacttag caaattcgtg
     6781 tcggtcctca tcatgcccat aaatgggcaa tactttaggc tgccacaggt gtttaggata
     6841 aagttgaaaa taatggcgca acacgcctaa atcggccacg tggtctggat ggtaatggct
     6901 aataatcact gcatccaaat cgagaggact gatttctttt tccagctcat tgagcgctcg
     6961 actaccagca tccatcaata actgaaagcc gtcatgtcct gtgaccaaat aagaggttgt
     7021 gcctgcatct tgatagggat aggctcccca agaccctaag gtggttaatt tcatctcaaa
     7081 cccttctttc ttgctttaac tcttattata acaaaaatta gtgtcaaaga ggattgagac
     7141 ccaccaaaaa caggtcctag cagataaatc tgccaagacc tgttcaacac aacttaacta
     7201 tttgatgatt agattactat tttgtattca aaaaactatc tttgtattta acagcgataa
     7261 tggtcgcaat gattgctttg atggtatctc ctggaataaa gaccaagttt gacaaaagcg
     7321 atgtcacaag agacatgcca gtgtaggctg ccaaccagat agcacccaga acatcaacca
     7381 gtacaactcc tgagattaaa agagctagga atgccaatgg cgtgcttttt gagaaccagt
     7441 tgcggtaaag taatgaaaag acaataggca caagaaggta ggcaataaca tagccagcag
     7501 atggccccat caatactgga atggttgttc gtgagcctga gaaaacaggt aagaaaaggc
     7561 caatcactaa aaataagaaa acagataagg ttcccttttt accacccagc atcaatccag
     7621 ccaacatgac acctaagttt tgtaaaacaa tagggactgg gataaagcct agcgggatag
     7681 caggaataaa gcctaaaata ataatcaaag tggtcatcat agccacctta acaagttctt
     7741 tggttgtaaa catctgttac gactccttta aaaaatagat atccttgtgc ctatgacagg
     7801 ctggattaaa gggactttga caataaggac agcatccaca accatactca gctaggctca
     7861 agagctttcg acaatgccca caaataacag gagatgtttc ttgatgtcct gttggcacaa
     7921 aggcatgatc tgttaactgg tcatggcaat ggtagcaagc ataataagtc tgacaaaaag
     7981 cacatttgag cccaacaata tcaagcgggg tatgataatg taggcaccga tactcttggt
     8041 caaggtcaat tccaaaacag tccgtcatat ctcatcctct tgtaacagtt taatcaaaag
     8101 catatgattt gtcaactcta tttttacaaa agggtaactg ttttctgaaa aagaacacgt
     8161 aaaacaagaa cttcccttta aagagggacc tatcagttga aagcaatagg acatccgtct
     8221 atacaaaaaa actgaaacca aagacgatag atcgccctca gttccaggat catagcattt
     8281 ttaattagaa attacggttt gatttattat aaccataccg ttcaagaaaa tcttggcgga
     8341 attcaagtaa gttatcgtcc ataatagcct gacgtacttt tttcatcaag ttaaccaaga
     8401 aataaaggtt gtggtagctt gtcaaacgaa taccaaaagt ttcgtccgct tttagcaagt
     8461 gacgaatgta ggcacggcta tagttttgac aagtgtagca gtcacagtca tgatcaagtg
     8521 gggtaaaatc ttcagcgaat ttggcatttt taatcacaag acgcccctca ctagtcatgc
     8581 aagttccgtt acgggcgata cgcgttggca agacacagtc aaacatatct acaccacgaa
     8641 tcacaccatc aatcaagcta tctggtgctc ccactcccat caggtaacga ggtttatttt
     8701 ctggtaagag gggagtcgta aagtcaagga cggcattcat ttcttcatgg ctctccccaa
     8761 cagctaaacc accaatagaa taacctggga aatccatagc caccaaatct gccgcagact
     8821 gacgacgaag gtcctcaaat ccagctcctt gtacaattcc aaataatcct tggtcatgag
     8881 gacgacgatg ggcttttaag ccacgttctg cccaacggct agtacgttca atggattttt
     8941 tcacgtaatc ataaggttgg taaaattgcg ggcactcgtc aaaactcatc atgatgtctg
     9001 agccaaggtt attctgaatg gaaatggctt tttcgggtga tagaaacatt tttgagccat
     9061 tcaagtgatt tttgaaagtc actccttctt cagtgatgtt tcgtgaatcg gctaaagagt
     9121 aaacttgaaa accacccgaa tcggttaaga ttggctgatc ccagttcata aacttgtgca
     9181 gaccaccaga gcgagcaatc aactcatctc ctggacgcaa ccaaaggtga taagtgtttg
     9241 ataaaataat ccctgatcca attgctttta actcttctgg agactgtgtt ttaacagtag
     9301 cctgagttcc cactggcata aacattggtg tcggaaaggt cccgtgaggg gtgataattt
     9361 cgcccaagcg tgcaccggtg tgcttttctg ttttgataag gcgatactta atcggataat
     9421 ctgtcatgtg ctttcttcct tgctatcaag ataaacagtc ttgagctttt ttgatctgcc
     9481 tgagtttcca tcataatgaa gggcttcaa
//
LOCUS       DAUVUY010000073         9481 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.073, whole genome shotgun sequence.
ACCESSION   DAUVUY010000073 DAUVUY010000000
VERSION     DAUVUY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.073"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..638)
                     /locus_tag="VOX58_001493"
     CDS             complement(<1..638)
                     /locus_tag="VOX58_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878712.1"
                     /translation="MERLGGADLDYCYHNHHDTAMISAEQLVFTYHDQKNPACQVSTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQADRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKT"
     gene            complement(575..1255)
                     /locus_tag="VOX58_001494"
     CDS             complement(575..1255)
                     /locus_tag="VOX58_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HES5878713.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            complement(1252..1845)
                     /locus_tag="VOX58_001495"
     CDS             complement(1252..1845)
                     /locus_tag="VOX58_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HES5878714.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPIVLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            complement(1842..3512)
                     /locus_tag="VOX58_001496"
     CDS             complement(1842..3512)
                     /locus_tag="VOX58_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878715.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     ETCGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGSLKEVNKK"
     gene            complement(3505..5268)
                     /locus_tag="VOX58_001497"
     CDS             complement(3505..5268)
                     /locus_tag="VOX58_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HES5878716.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLLLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQAIERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLLQETINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLNTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            complement(5265..6101)
                     /locus_tag="VOX58_001498"
     CDS             complement(5265..6101)
                     /locus_tag="VOX58_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878717.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDLQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            complement(6098..7120)
                     /locus_tag="VOX58_001499"
     CDS             complement(6098..7120)
                     /locus_tag="VOX58_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HES5878718.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYGAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            complement(7122..8006)
                     /gene="isdE"
                     /locus_tag="VOX58_001500"
     CDS             complement(7122..8006)
                     /gene="isdE"
                     /locus_tag="VOX58_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HES5878719.1"
                     /translation="MIKRCKGIGLALMAFFLVACVNQHHKTAKESEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSANPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHS"
     gene            complement(7990..8865)
                     /locus_tag="VOX58_001501"
     CDS             complement(7990..8865)
                     /locus_tag="VOX58_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HES5878720.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDNQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            complement(9062..>9481)
                     /locus_tag="VOX58_001502"
     CDS             complement(9062..>9481)
                     /locus_tag="VOX58_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HES5878721.1"
                     /translation="KETNNPQENSHLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLT
                     DLLAKKATGQSTQETSKTDDTDKAEKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSN
                     QQSNGEESSSRYHLIAGLSSFMIVALGFIIGRKTLFK"
BASE COUNT         3114 a         1977 c         1625 g         2765 t
ORIGIN      
        1 gtttttcctg ctgcttttaa ttgggtaaga taggctttaa cagcagctat gcctgcacta
       61 tctaagttag cagttggctc atcaaaaagc ataatattag taccttgcat aatggccgtt
      121 gcaatagcaa ctctttgttt ttgccctcct gacaatccaa acatatcctt gttaaggagc
      181 tctgcaaaag caaaatcttc tgctaaagtc caaagacgct tcatgataac ctttgcatct
      241 aaaccttgat tttcacaggg aaagaccagt tcgtgttgga cttcgcgata gaaaaactga
      301 ctggctggat tttgaaaaac agaggccaca gatctagaaa atgtttcaac actgtctctg
      361 tctgcttggc aatcagccac atctagtcta ccttcatatt ttccagcata ataatcagga
      421 attatgccat taagaagttt taaaaaggta gatttacctg aaccagaagg tccacacaaa
      481 acaatgaatt gtccagaggc aatttgacaa gtcgatacct gacaagctgg atttttttga
      541 tcatgatagg taaaaacaag ttgttctgct gaaatcattg cagtatcgtg atgattatga
      601 taacagtaat ccaaatctgc accccccaat ctttccatgt taagtgtgag ctaaagcatt
      661 cactggtgcc tttgttgact gctagccctt tagttagact agcgatagtt agttcttggc
      721 tacttcttat caaagataaa agcaatggaa ccattaaata ctccaagtat tgtttaggtc
      781 tgatcaaaat agaccatttt gttaaaataa tccccctgat tgttaaagag cgatgaataa
      841 cacaacattc ttgtctaatc attggtatga aacggcacat aacggctaag gtcaatagcc
      901 agacttctgg gaaatgccat ttgcgtaatc catgaaccaa ttcgtagatg gtagttgtct
      961 taatagtaat taaacctgct agtaaagaag ggaaaactaa ccttcctgct attgagacaa
     1021 aactcaataa gtgatcaaga taagaaggga aaatagatag cgggattaca gatagaccga
     1081 tcattaacag ataaacaacc gccaacttga atgctagctt tttcttattt aaagcagaca
     1141 aaagtaataa gagaaaaata acaattataa attctaaaaa gccatcaaca cggaaaaaga
     1201 agcaggcatt ggcaagtacc aacaaaagca gtttggtgcg aacatcaagc ctcatgataa
     1261 caatcctgat ttgataaaat gtttctttag taaataatgt gcaaaagctg accctaataa
     1321 ggctcctgca agaacaattc ctacaaacca aataacatgc cctaaactaa aatcatacat
     1381 aatgcgattg acataggtca tacttttacc gcgggctaac aaactttcaa cataggcatc
     1441 tttcaacaac cacatcagca caatagggcc actattgaca aaagcaaaaa ccatataaga
     1501 taggtcatta aaaaaccttg acttgtattt tcctaaagaa gcaataatat ccgctattaa
     1561 accgaaaata atgctgggaa acatcgcggc aaaaaagtga ccactaagga agaaaaaacc
     1621 tcccatcatc aatcctaata atgttattgg cccaaatgtt tgcaatttag caatcaaaag
     1681 aaaataaatt gttcccccca gtaaagctgc aaaggcaggg gcatataaca tatttcctga
     1741 gcgatcaaaa aagatgttaa taaacgtgcc tagaccaaca caaataaagt agagcgcggc
     1801 aaatgcgcct gttatcataa tatcttttat tgttaactgt ttcatttttt attcacctct
     1861 tttagggaac cattttttac gcaaaataga tgatcagcac aagctagggt tgatggcctg
     1921 tgagaaatta agaagaccaa tccttgacaa tgtgttttaa ttaaatcaag caagatagct
     1981 tcatttagag aatctaaatt tgacgtgggt tcatcaaaaa tatagcaact cgcatctttc
     2041 aataaagctc gcatcaactc taaacgttga ccttctccag ctgaaaagtc agaaggactc
     2101 ttgataatcg tatccaaacc ttccttacaa gctaacagtc tgtctttcat accacaagtc
     2161 tcagctaaat tccaaatgga ctcatctgaa atatcttgac ggccaaaggt aagattttca
     2221 cggatagact gttggaaaag ctgcgctgtt tgtggcacat aagcaatagt atcttgtaat
     2281 ttagcagcat ttagtaagca actattccta tcattaagaa aaatatctcc tgtcttccaa
     2341 ttataccatt tcataattaa tttcaccaaa gttgatttac cagatcctga ttcgccttta
     2401 atgccaataa tccccttttc ttgaaaggta actgataaat ccttaaaaac cagctcttgg
     2461 cgctttggat aagcaaaact gaggtcctca aaagaaacac tccttaaacg atctatctgt
     2521 tttgtgcctt cgtctactat gacattttcg tctagaaggt caaaaatatt tcgagccgca
     2581 ttcatagcac gcttaaagcc taatggcaaa cgtcctagtt ctaaaaaagg agaaaacgaa
     2641 gcagtgaaag caataaaagt taataagcct ttatcaaatg ataagctgtc atggcatatc
     2701 ccatcaaaaa ccatataaga aaatgctaag atcattaagc caagccaaaa aaaagtcaat
     2761 gccatttgca aaaattgggc ttgcgctacg ttacgatcaa gagcatttac atgattactt
     2821 ttttttatta atcgttcaaa ttgctcgtct aatacctgaa attgcaaaag atctttgact
     2881 gatcgtaaac tctctaaaaa ataagacagg taatcttttc gaccttcatt ttggcttttt
     2941 agtaaaacct gtaaaatatt agcaaaataa atgggtataa caatggctaa acaagcatag
     3001 gttgccaaag ctaataaggc taactgccaa ctactttgac aaaagtacca gaacattaaa
     3061 cctgcagaca atattgctgt acaaataggt gcgattgtgt gggcaaaaaa aacttctaat
     3121 gcctcaatat cttctccaat cattttaagc aaataaccac tgtcttgact atccaagcaa
     3181 gctggagaaa gagctcttag tttcttaaag atcaagttcc taaaagctgc taaactatga
     3241 aaagcaacaa aatgaccaaa ataatgctct ccataacgaa caaaacctct taaaaaggct
     3301 agagcaatca aaagatataa agctaatatt ggaatcactt cttgtctaat agctagtagt
     3361 cctaaataag ctattccagt tggaatactt accgtgatga caaaacccat aacagcaaag
     3421 ccaacagcca agataatcca aggtagcaga gttttcatta gggttaataa acgccaaacc
     3481 aggcttaagg tagatctgtt aggtttagcc ataaatactt gcctccagtc ttgcttgagt
     3541 atcaactagg tgacgataag ttggttggtc ccgataaagg tcgcaaggtt tgcctaaaca
     3601 agcaggctgc cctgtgttta aaaagagtac ttggttagct cctttcagtc gagacatctt
     3661 atgagtaatc acaataacta ttgctgtctt agctaatctc gttattaaat tgtcaataat
     3721 acgttcattt tctgcatcta gactagatgt tgcctcatca aagatataaa gagacctctt
     3781 acttaataga gctcttgcgc aaatcacctg ttgcttttgg cctggtgata acaggttacc
     3841 attttcacca actattgtgt caagaccatc tggtagccag ttgatgaagc ttaacaaacc
     3901 atgttgatct atccaatcta agatttcttt tttagagaga tttgctgcta accttaaatt
     3961 atcgtaaatg ctacggttca ataaggtaga ttggtccgat acataaagaa cctgctggtt
     4021 gatagtttct tgcaacaaat tgtcactgtc taaaccgtca aataaaatat gcccagtcgt
     4081 tgcagactgg cgtttcaaga ggagttgagc tagacttgtt tttccttgtc ctgacacccc
     4141 cgcgatagct gttagttggc ccttcgttaa tgtcatggta accccactaa gcactgtttt
     4201 tttctcataa gctaaagaaa tattttggat gtcaatctgg ttaaagttta ttgctgtttt
     4261 actcttgtta tctttccttg ctggaacact atccaagaaa ctaaagatac ggtcagccat
     4321 tttggtattc atcatgacca agtgcatgcc ataaccctgc tcacgaatcg gtgtaaaaaa
     4381 ttctgtagca atcaagagaa aaaagaggaa attaaaaaag gataactggc ctgttgccaa
     4441 agcttgaacg gctaaaaaac ctgacaaacc aattcccaga tacataactg catccatata
     4501 accaactgct tgtaactgaa agccaagcaa agacatggta gcctttcgaa actgttctgc
     4561 cttacccgaa aattcttgct cgtaacgttc tattgcttga taagaataca gtgtattaag
     4621 accttttaag tcatccataa ataaattacc aacatccata taagaagacc aataatgact
     4681 cataatttgc ttagaccgct tttgcatagc aacgattgaa agtggaatca gaagcagaac
     4741 tcctaaaaag acaaggcctg ccaatggata aattaaaaaa actagtatca taatagttgt
     4801 gcagttccac ttggttctca tagacaaaga caaatagtaa ccataataag tatccaaact
     4861 atcaatcccc tgtgaagcca acgttaaaat atcagcatca ctggcatgag catcaaattg
     4921 cccgtctaaa tcaataaaag cctcaaagaa agattgtttg agtgaatctc tagcaaattg
     4981 tgaagcgatc ccttgcagtt gcttgcctaa tatagcaaga acaaagccta ccacatttaa
     5041 aagtaggagt aaacctgcca agcgtcctaa ggcaatagca tgacccaaaa taaaggtcga
     5101 aaacgttttg gcaatgaggt aaaaactgat catcctcatc acaaattgta accaagataa
     5161 aaaggctgac acataaagca aatacctttt tggtgcaatt cgttcacgta atcgttttaa
     5221 cagtcttttc ctttttcccc ttgatattgt atcttctgtt tccatcatct gttcttgttc
     5281 ctatctctcc tttgagttct aatatcattg gtttcattga ccaatgaagt gaggacaaac
     5341 tgttgaccat ttctctcaac aaagggcaat tcaacaccaa agacatctct gataaactgt
     5401 tgatcaagga ggtctcttgg ttgcccttgg gcataaagtc gcccatcttt cagtccaata
     5461 agatggtctg aaagagacaa ggcctgattg atgtcatgta atactaacac aatcgttagg
     5521 cctagctctt ggttgatttt agtaatcagc ttgagaatgt caagctggta cttaatatcc
     5581 aaataagtcg ttggctcatc taaaaaaata atatctgtat cctgggcaag ggccatggca
     5641 atccaaaccc tctgctgttc accaccagaa aggtgttgga ggtcacgata aacataatca
     5701 ctcaaagccg ttacttctaa ggcccaagca aggcgttcct catcttcttt ggtagtagtt
     5761 ttaaaaaaag aacgacgggc aagcctagcc atggctacca attcacccac taacaaacct
     5821 tcgaccactt gatggtattg gtgtaccact gccacttttt tcgcaaaagc cttgagagaa
     5881 atcgttgcta attcttcctg ctcaagccaa acctgccctt gttttaacgg aaggtttttg
     5941 gttaataatt gtaataaggt tgacttgcca ctgccatttg cccccataat agtggtaatt
     6001 ttcccttttg gaatgctaaa ggtcaggtca tctaaaacaa gtttttcctc ataagcaaag
     6061 gaaaggtttt gcacctgaat atgactgctg ttcttactca tataatatca gacctcctta
     6121 gcaggtaaat aaagtaaggc ccgcctacaa tactcattat aatcgcaggg ctaatttcca
     6181 atgggtaagc aagacttctt cctaaagtat cggctagcaa aaagacaaag gctccaagca
     6241 aagctgaaaa aggaattaag atttggtgct tacttccaac cagcaagcgg ctcatatgag
     6301 ggacaatcaa gcctaggaaa gaaaccactc caacgatcga agtggctacc gaagccaata
     6361 aaacagcaac caaggaaatt cccaaccgta acgctgtagc atcgattcct aaatgacgaa
     6421 tcacctgatc atctaaaaga agaaggttac aggtttttga caataacaaa gctagtaaca
     6481 agccaatgaa tgtataaggg aataaaaccc ctacatcagc ccatgttttt tgactaatat
     6541 gacctgctaa aagagcattg accatgggac tagctgaggt gaaaaagctg gttaaagcag
     6601 ttgataaccc cataaataaa gcattaatag caatccctgt tagaataagc cgaataggat
     6661 taagcccctt cttccatgct aaaccataaa tcagcaggaa agacactccg cctcctaaaa
     6721 aggatacgat tggaccatac agcagcaact gcggtagaag taaactagag actaaaacca
     6781 taaaactagc accactacaa atccctataa tagcagggtc tgaaatagga tttttcaaca
     6841 ctgcttgaaa aagaactccc gaaaccgcta tccctgctcc tgctaataaa gcaatgacaa
     6901 ttcttggaaa gcgcaaatca taaatcaaag ccacttgggg gtcataagcc acaaacagtc
     6961 ccttgacaat tgccccataa gaaaccgcca aaccaccaag ggataaggca ataaccgcca
     7021 aaaacaggag actgagaatt aaaagaacta gataaagacc acttctgaaa cgttttttag
     7081 ccagtaggga ttgatccctt tgtaaatctg atgctgtcat gttacgaatg atctcccacg
     7141 tggtcaaaaa gctgtgttaa ggtgtccaag gcttctgggt agttcaattt agcactcatg
     7201 ccaaacaggg tattgtccaa atcatagact ttcccttcct tgactgccgt aaaatgtttc
     7261 caaatatcat tttcagcaaa ttctttgtca aacatcactt ttaccttgtc tggaatggca
     7321 tgagctgttc gtaaaatcaa gtcaggctcc ttagccagca tgtcttcagg attagctgat
     7381 agaaattctt tctcatctga ctgataaaca ttctcaccac ctgccaagtc caaaagattc
     7441 cctacataag attggttcgt cgccaccaaa taactacctg gcaagcccat aagaataagc
     7501 actttaggct ttttcttccc cttacgttta gcttggaaag cacgataata gtcctggtat
     7561 tgctggcgca attcttttgc ttcttgttga cgttggaaaa ggttccctaa atcatcaatg
     7621 gactggtaca tgccctcaac actccgtaag ttcaaaaaac catactcagt atccaatttt
     7681 tgatacttgg gttccaaatc ttcttgtaaa gaattgggac tcaaaatcca agttggtttc
     7741 aaagaagcaa tcaactctat atcaggattc atgggtaaac ccacacgctt aacagcatca
     7801 tagcgtttag gaagggtata taatttacta tcacaaaccc caacgaggtc taaatttaaa
     7861 cggtcacaga tgtcaaccac agcaaccgaa gtggctacaa ttctctgctg ttcagactct
     7921 ttagccgttt tatggtgctg attcacacaa gctaccaaaa agaaggccat taaggctaga
     7981 ccaattcctt tacatcgttt tatcattttt tttcctcttt ttccaaaccc atacagcaaa
     8041 ccctcctaac atcagaaaaa aagttggaaa gtagtaaatc atgattgata agtctttttt
     8101 agaccgaaac ttatcttctt tttttaactc taaagaagtc gttaagccca tcttagaagg
     8161 aagaattttt ttatctgata gactcttgtt atttgatgag ttggctttag gcttattctg
     8221 agttatcata gcacctgtgt gagactcatc ttgactttct cctagttttg tatcaacagg
     8281 cttttgattg tcttttactt cttgattttg agaattgtct gtttcagtaa caagctgggc
     8341 caacatatta cctgaatact tttgtattaa ctctgaagct gacaggtaaa agaccacttc
     8401 tctgcccata ggttcaacaa acatgcttcc tcgaatgata ctatttacag taggcaaact
     8461 aatagcaata tctagtgttg tcccattggt atcagtacct ttttgggtga tattgtaatc
     8521 aactgcctga aagcttccag ttcctccagg ttgtatccag aactgataat tgcccgaata
     8581 atcagctaag ctcatacgaa aagtaagaac gatctttcca gcatcagata cttctagcat
     8641 ggcatcacta tagacagtcc cttcaaccat accttgacca atatcaaagg aatgctcacc
     8701 tccgctatct tctatctgcc cagatatcgg atgacgataa tttctctgaa taatacatcc
     8761 ataaatttga cctttatctg catacacaac acctgtcatt gtcgataaac ttatcatgaa
     8821 cactactatt acctgcaaca attgctttat tactaccttt gtcatatgct cctcttgtct
     8881 atcttttctt aaacatctta tttcattttt taaatgattc ccattttata acatctccaa
     8941 atgtgaaaaa cacacctatc ttgcgactac ttcaacctgt atcaggagtc ctgtcggaaa
     9001 taggtatgtt tttagctctt gctgactagc ttttttgtta tttcacttgg tactaagttt
     9061 cttatttaaa taatgtcttt cgaccaataa tgaatcccag agctacgatc ataaagctag
     9121 atagacctgc aattaagtga taacgagagc tactttcttc accatttgat tgttgattgg
     9181 aacgatgttt cttatcagat tttttagtct tagtatcttt tgccgtttta ccttcaattg
     9241 atgtttgatg gtcacgcact aactgtttca atttctctgc cttatcagta tcatctgtct
     9301 tagaagtttc ttgagttgat tggccggttg cttttttagc caggagatca gttaagttag
     9361 caataccagt agctgctgaa ggaggacttg taggtgtttt ttcttgttga cgattttgag
     9421 gtcctttcaa ctgatctgtt gaagttagat ggctattttc ttgtggatta ttagtttctt
     9481 t
//
LOCUS       DAUVUY010000074         9419 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.074, whole genome shotgun sequence.
ACCESSION   DAUVUY010000074 DAUVUY010000000
VERSION     DAUVUY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.074"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..757)
                     /locus_tag="VOX58_001503"
     CDS             complement(<1..757)
                     /locus_tag="VOX58_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HES5878722.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYA"
     gene            complement(855..1181)
                     /locus_tag="VOX58_001504"
     CDS             complement(855..1181)
                     /locus_tag="VOX58_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HES5878723.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            complement(1165..1599)
                     /locus_tag="VOX58_001505"
     CDS             complement(1165..1599)
                     /locus_tag="VOX58_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HES5878724.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            complement(1592..1828)
                     /locus_tag="VOX58_001506"
     CDS             complement(1592..1828)
                     /locus_tag="VOX58_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HES5878725.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEITLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            complement(1833..2261)
                     /locus_tag="VOX58_001507"
     CDS             complement(1833..2261)
                     /locus_tag="VOX58_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HES5878726.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            complement(2236..2562)
                     /locus_tag="VOX58_001508"
     CDS             complement(2236..2562)
                     /locus_tag="VOX58_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HES5878727.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            complement(2564..3550)
                     /locus_tag="VOX58_001509"
     CDS             complement(2564..3550)
                     /locus_tag="VOX58_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HES5878728.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQDLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDI
                     MAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELE
                     IYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
     gene            complement(3534..4472)
                     /gene="tadA"
                     /locus_tag="VOX58_001510"
     CDS             complement(3534..4472)
                     /gene="tadA"
                     /locus_tag="VOX58_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HES5878729.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYVRLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            complement(4565..4930)
                     /locus_tag="VOX58_001511"
     CDS             complement(4565..4930)
                     /locus_tag="VOX58_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HES5878730.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            complement(5082..8723)
                     /gene="rpoC"
                     /locus_tag="VOX58_001512"
     CDS             complement(5082..8723)
                     /gene="rpoC"
                     /locus_tag="VOX58_001512"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HES5878731.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDRLDINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAVFTAEAE"
     gene            complement(8814..>9419)
                     /locus_tag="VOX58_001513"
     CDS             complement(8814..>9419)
                     /locus_tag="VOX58_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HES5878732.1"
                     /translation="FDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGVM
                     YMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVLQ
                     EILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDNE
                     VELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
BASE COUNT         2792 a         2000 c         1712 g         2915 t
ORIGIN      
        1 gagcataatc ttttaaccac tgtttcaata actggctctg agggctcgtc aataaattga
       61 ctggtgccag aaaaatcgca aaaccgtctt ttttcaagta ttttaaagac tgttccatta
      121 ataaatgatg ggcataggta tgcttatctg aactagccac cttgtaccgt ttggcaatat
      181 catcgttagg ataataacca actggtaagt cactgatgac aatgtcactt tcttttagta
      241 attgaggcct taccgcatct tcttgaataa aatgagctga agaatccatt atctcagcaa
      301 tactggctga cagatcaatc aagagatcat caagttcaat ccctacataa tcaaggctct
      361 tgctcgtgtt gttgagaagg gtttgggcta ggttccctgt tccacttcca atctcaagta
      421 cctctaagct atctttatca ctcaattgtt ccaaaagata cagcagaatg aagccaatag
      481 catctggtgt gaactgatgg ttggcttgga gttgctccgt ctgagctgcc ttaataaaaa
      541 gaaactggta ggccttacgc cattcttctt ttgtcaggca caaggctttc agtttatcac
      601 tattttgagc aacttgagga ctagctccct cagcccctaa ataaaaggag ttttgttcaa
      661 caatggcatc gtatatatgt gtttttagat cattttcaat tagttgacag ttctctaaga
      721 gcagctgata agcttcttca attttttcaa aagtcataac cttatcatac caaattacac
      781 ttgaaatgaa aagaagtgtc acctttaatt actatatatt ggatcatttt atggatttat
      841 gacaacaata ctatttattg actatattgt ttgggtttaa ggtattcaaa ctgatactgt
      901 cgttgctgat ctaacttaac aattaacatc catctctctt ttcgctctcg acaaatgact
      961 tgaccacctt taaaaaagac aggattagaa gctttatatc tatgatcttg ataaactatt
     1021 tctgctatca gtcgggcttt aacatagtca gtttgagctt ggtattcctt gtgtagagct
     1081 gtcacttgag aaaggtaaac ctgtaaaacc agcaaaaaaa tactagccat aaacatagca
     1141 tacaaaagga tgcctgcttt gagattagtc ttgaaaggca taataaaaag tcctttctaa
     1201 cccacttttc cactttaaag taatgcaaat ctgtgactgc tctatgtgaa tatgactacg
     1261 tgatattcca aataacatgg gttgataccc ttttccatta ctagctgatt ttcgaaaatc
     1321 atgactttta aattggccaa aagctagtac tttctttcct ttttcaacgt atagtttatt
     1381 atcagctact ttgtaaaatc ttgctccact taactcctct cgcaattgat gagaaaataa
     1441 gagccaattg tcttgatcat gacgggctag ataatggcta cgtttaagga gggtttgggt
     1501 caaaccttga taaaccaata aagaccctga tatcacgagt aaggctatta acgcctctag
     1561 aagggtaaaa gcttttatgt tacttaattg tttactcaat cttcatcact tctccttcgt
     1621 catcgtgaat actaatgtat ctatccgtac gtaaaatagt aatatgacaa ccattcaatg
     1681 ttatctcttt agttctagtt tgtactgcca tcaaggctag attaagcttt tcttgctgct
     1741 ttcgatagta cgttagagca gcctgactct gctgtaaact gcttaagacg agaatgacaa
     1801 tactaaagag gatgcctgtt gcgatcatac tttcaagggc tatgtaagct ttgacttgtt
     1861 ttttgataat tcccactccc tagataaaat tggtatttaa agcgtcgatc agtcatgtca
     1921 aaaataattt tagctaagga atggttgcct cccatctgat caataactaa ccgataagga
     1981 tgattcactg ttattgattt aggaactgtt aggctattac cttctgtaac aatttttgtt
     2041 gaagaaatat ccaagacgcg ctgtttttgc tgtaatatgg ctaacttttg ctgatgtcga
     2101 tacaaatgtt caaaatggct aaaaaacaga tgctcttcta ctttttgata gctttttgtc
     2161 actggtacag acaatcccaa tataatgaaa ctcatcactg acaaacttag taaagtttca
     2221 agcaaagtaa aagctttaat tgctaagacg ggctttttca tttttatgtt tgtcataata
     2281 gtcttgataa gctttttctt gtttctcagt gatactacca tctgccttta actggctcaa
     2341 acttggtttt gagccttgag ataattcata tagttctgct tgattctcca ctaatttaac
     2401 aacagcggca ttacctgttt ctgtaaccct gtctttttgc ttgcttaaat taggcacaaa
     2461 taatagcatc aaaacactga tgacaagaat caccaataac atttctagaa gagtaaatcc
     2521 ttttagcttc ttgtgtcgta agttgttcca ttgattaatc atcttaaaaa atacctccca
     2581 tattttggta aattggtaat aggattgccg cataaatcat gacgattgtc acagcaacta
     2641 ctaaaaaaat agctggttga ataagttgtg tcacttgata aagctgacta aaaaattgtt
     2701 cccagctttc ctgggcatag atttccaact cagcccccag tttcgatttg atttcaccgt
     2761 attcaatcat taaacttaat tcttttttaa aaaaagggta ggtagctact ttaatgtgaa
     2821 aggcttgccc ttcaagcaga ctcattctga tgtcctcagc taattctttc attagtgagg
     2881 atttctcaat ggccataatg tctaaaatag tcatcaaatc aagaccttga cctattaacg
     2941 taccccattc tctagcataa taactagtta agtattgttt taacagttta cctaaaaaag
     3001 gataacggct tagccgacta tagagtttca agcggctttg agatcgccat cgtagccata
     3061 ccatcccgaa caacaaaata agacctgagc aaaaacctat aaagaaggca ggaaaatggt
     3121 taagaaagta agttatctga ttttgagttt ctaattgagg gactaaatag cgtctcaacc
     3181 ctagcatcat cacaaaaaga aaaagtaaaa gaataagagg ataggtgata acttcaacag
     3241 tttttcgtct gatccttgcc atttggttca agtaatgttg aatcgctact aatgttgttt
     3301 caatattacc gtgtctatca gctaaactaa tttgagtaag aatagcatct gaaaaaccta
     3361 aacccgacaa catatctgct aagtcttgtc cttttaataa agactcttcc attttaagaa
     3421 cataatctag ctgaagcaac tggcttcttt ttaaaaaagc aataacctct gcgaagctaa
     3481 atcctgtaga taaaagatta gctagtaatt gaatgaattt atgctgatgt ttactagata
     3541 attttttcaa gcttagcttg cttgggattg agatgtcctg cctcaagaag ctgatcaatt
     3601 tgttgattcc agttcgatga ggaataatat tcaaattcgt tttgagtaga gtcaatcaat
     3661 gctcctccat taagtaacct ttggtaagca attaatgcta agcaattaga cagttctgct
     3721 ttcgttacac caagttctaa caatcttaca taaacacccg agatactttt agcgtgaacc
     3781 gttgaaaaaa ccatggcacc tgttagacta gccctaataa cggctcttgc tgtttgacta
     3841 tctcgaatct caccaataac taaaatatct ggtcgatggc gtaaagacag tttgatcaaa
     3901 ttgtcataag tcataccaat atcatcattc acttgtaatt gtaaaatctg gtgattttta
     3961 atttctacag gatcttctat actaataacc tgtgcttctt gatgataact cgaaatcagc
     4021 tggtacatca aggttgtctt cccagaccca actggtcctg caaataaata caaccctcta
     4081 ccgccaacct gattggctac ttttgtcaat ccatcaaacc aataatgtac acttttattt
     4141 tgatgatgaa gcagacgaat cactaagctt tcttgcccgc gataatcacc cacgctagat
     4201 aagcgcaaag ataactgctt atctttacta agcttataat cacatgaacc cacctgacaa
     4261 cgtctctttt caccaactat cattcctgca acgaatttaa agtgactaat aagaggagcc
     4321 atccgatcgc tctgataaac atcaactaat ctcctttcat ctcctattcg taaaaaaaga
     4381 tcatattgat ctgctcttgg caaaatataa atatcttgtg cctgaacctg ttcagctttt
     4441 gctagaatag cttttgctaa tgcttgtacc ataattcctc cttactcatt tattcgtaaa
     4501 caaaaaaaga aaatatcaga agaagtgata ttttctttta acttattttg tttttaacaa
     4561 acacttaaag gtttaacttg caaagataag ctacctgtgg tgaatcattt cgttgttcaa
     4621 attcagggat ataattattg cttggtacaa tatctttatt tttaaggagt aaaagagaat
     4681 gaaactgctg taaatcttcg tcacagtcct cgcaccatct tttatcttct ttttcccaaa
     4741 aagtagctaa caaattcttt tgactatgat aactaggaaa aatagctttc atgcgctgcc
     4801 attcttgatt aaaataatca agcgcttctt gccagtcact aaattgttgc tcatctataa
     4861 tatcgtcttg ccatccatcg atgaaccacc aaggctccca atcaccatac attttaatta
     4921 cttgatacat aacacattac ctattcctct tatgcattat tatagctaaa tcataagtat
     4981 tttgaaaaac atctgctcat tgggactaag actgattgtc aatgcttttt actgccaaca
     5041 aactcagaca aaaaagagcc cctaaggact ctttcatgag attattctgc ttctgctgta
     5101 aaaacggctt ctgctgagac ttctgtatga tcaatcactt caatctcatt catcgcttgt
     5161 ggttcaatgt tacggtagcg cgccatacca gtaccagctg gaatgatttt accaatgata
     5221 acattttctt taagaccaag taagtggtct ttcttaccac ggatagcagc atctgtaaga
     5281 acacgagttg tttcttggaa ggatgcagct gataagaagg aattggtttc aagggaagcc
     5341 ttagtaatac ccataagaac aggacgagat gtcgcaggaa taccaccaga gataacaata
     5401 tctttgttag catctgtgaa atcagaaata tccataagtg tacctggaag gaggtctgta
     5461 tcacctggat ccatgacacg aactttacga agcatttggc gaaccattac ctcaacgtgt
     5521 ttgtctccga tttctacccc ttggctacgg taaacttttt gtacttctgc aagaaggtac
     5581 gtttcaactg acaaggtatc acgcacttca aggagacgtt tcggttgaat tgacccttct
     5641 gtaagggcag ctccacgatt aacttcgtcg ccaacttcaa ctttcatacg tgctgtaaat
     5701 ggtacgacat attcgcccat gccagttttt ccttgaacgt agactttctt ggtacgagtt
     5761 gacgcatctt cttcaatctc gacgacattc cctttcactt cagtaatgac cgcttcccct
     5821 ttaggattac gtgcttcaaa gatctcttga atacgaggaa gaccctgtgt gatatcggta
     5881 tttgaggcta caccacccgt gtggaaggta cgcatggtaa gctgagtacc aggctcaccg
     5941 atagattggg cagcaatagt accaactgct tcacccactt caacagcatc accagttgcc
     6001 aagttgatac cataacagtg acggcagaca ccatgacgag tcgcacaggt aaagacagaa
     6061 cgaatggtca cttcttcaac acctgcatca acaatcttac gagccatgtc ttcagtgatt
     6121 aattggtcag caccaatcaa gacttcacca gtttcagggt gtttgactga tttacgtgtg
     6181 taacgacctt gaagacgctc ttcaagcgtt tcggtaactt ctttaccatc tgtaatagca
     6241 cggataagaa gaccacgatc agtgccacaa tcgtcctcac gaatgataac atcttgggca
     6301 acgtcaacca aacgacgagt aagataacct gagtcggctg ttttaagggc cgtatcggtc
     6361 atccctttac gtgcaccgtg ggttgagaag aacatttcca aaacgctcaa accttcacgg
     6421 aagtttgaca agataggaag ttccatgatg cgtccgttag gagcagccat caaaccacgc
     6481 ataccagcaa gctgtgagaa gttagagatg ttaccacgag ctcctgagtc catcatcata
     6541 acgattgggt tcttaggatc ttgtgtttca attagacgtt tttcaagggc ttctttagct
     6601 tcacgccatg ttgttgtaac ggcaacataa cggtcatcat ctgtcatcaa accacgacgg
     6661 aaggctttgt taatttcttc aacacgatgg tgagcagcat caatgatttc tgctttgtta
     6721 tcgataactg ggatgtcagc gatacccact gtcaaaccgg caagggttga atggtagtaa
     6781 ccaaggtctt tcaagcggtc aaggaaggct gatgtttctg ttgtacggaa acgtttgaag
     6841 gtttccgcaa tgatgttacc gaggtttttc ttcttaaatg gcacattgat gtctaagcga
     6901 tcaatcactt cttggatgtc ttgaccaggt tcaaggaagt atttatcagg tgttccttct
     6961 gtcaagttgg cattgtttgg ctcttggagg taaggaaggt cctctggcat gatatcgtta
     7021 aagagaatct taccaacagt tgtcaccatg attttatgtc tttgactgtc tttccaaggt
     7081 ttgtttggca tgctgtcaac agcgataccc acacgactat gaagatgagc ataaccatta
     7141 cgatatgcca tcacagcttc gtctttatcc ttgaaaatca taccttcgcc ttcacgacca
     7201 gcatcttcca tcgtaagata gtagttacca agaaccatat cctgagatgg agtaacaact
     7261 ggtttaccat ctttagggtt caagatgtgc tctgcagcaa gcatcaaaag acgagcttca
     7321 gcttgtgctt cttctgaaag tggcacgtgg atggccattt ggtctccatc gaagtcggca
     7381 ttgtaggcct cacacacaag tgggtgaaga cgaagtgcct taccgtcaat aagaaccggt
     7441 tcaaaagcct gaataccaag tctgtgaagg gtcggtgcgc ggttaagtag gactgggtgt
     7501 tctttgataa cttcttctaa gatatcccag atgcgttcgt cgccacgttc aaccatacgt
     7561 ttagcggctt taacgttacc agcatattct ttggcaacaa tttcgcgcat tacaaatggt
     7621 ttaaaaagct cgatagccat ttcacgtggg acaccacatt gatacatttt aagggttgga
     7681 ccaacagcga taacggaacg tccagagaag tctacacgtt taccaagcaa gttttgacgg
     7741 aaacgccctt gtttaccttt aagcatgtgg ctcaatgatt tcaatggacg acttcctggt
     7801 ccagtgattg gacgaccacg acgaccatta tcaatcaaag catcaacggc ttcttgaagc
     7861 attcgttttt cattttgaac aatgatgcca ggggcattaa gttctaacaa acgtgccaaa
     7921 cggttattac ggttaatcac acgacggtac aagtcattca agtctgatgc cgcaaaacgg
     7981 ccaccatcca attgaaccat cggacgaaga tctggtggaa taaccggcag gatgttaaga
     8041 accatccatt ctggtttatt tccagatttg ttaaaggcat caaggacgtc taaacgacga
     8101 actgctttaa tccgtttttg accagaagca gatttcaact cttcttttaa ttcagcgatt
     8161 tcagctgcca agtccacacg tttcaagaga tcttgaatgg cttcagcacc catcttagcg
     8221 acaaatgacc catgaccata ctcttgtagt ttttcacggt attcacgttc tgttaataat
     8281 gattttggtt caagcggtgt atcttttgga tcaatgacca cataagccgc aaaatagatc
     8341 acttcttcaa gggcacgagg actcatatca agagtcaatc ccatacgaga tggaatccct
     8401 ttgaaatacc agatatgtga tacaggagct tttaactcaa tgtgacccat acgttcacga
     8461 cgtactttag cacgagttac ctcaacccca cagcggtcac aaacgatccc tttataacgg
     8521 atacgtttgt acttaccaca cgcacattcc cagtcttttg ttggaccaaa gatgacttcg
     8581 tcaaagagtc cttcacgttc tggttttaat gtacggtagt tgattgtttc aggtttttta
     8641 acttcaccat aagaccatga acggacctta cttggtgagg ctaatgtgat ttgcatactt
     8701 ttaaaacgat ttacgtcaac cactagtttt acctttctgc ctttgatgac tttaccagtt
     8761 cctggggcct ttgccccaga ccagtaaata attatttaat aagaattgat tgcttatttt
     8821 tcgtcagttg tttcagaaac ttcttgggtt tcttgagctt gtttttcacg tgccttctcg
     8881 agatcgtcaa catgcataat gtcatcgtct tcaccttcat caagatcacg aagttccact
     8941 tcattatcat cctcgtcaag cacacgcata tcaagaccaa gcgattgcaa ttcttttaca
     9001 agaacacgga aggattctgg tacacctggt tttggaattg gtttaccttt agtaatcgct
     9061 tcataggctt tcaaacgtcc tgtgacgtca tctgacttgt aggtcaagat ttcttgaaga
     9121 acatttgatg caccataagc ttcaagggcc caaacctcca tctcaccaaa acgttgtcca
     9181 ccaaattgag ctttaccacc aagtggctgt tgggtaacaa gtgagtatgg tcctacagaa
     9241 cgggcatgaa gtttatcatc aaccatgtgg tgaagtttga tcatatacat gacaccaacg
     9301 gatacacggt tgtcaaatgg ttcaccggtg cgaccatcat aaaggaccgt cttagcatcg
     9361 ctatccatac cagcttcacg aacagtgtcc caaaggtctt ctgatgaagc cccgtcaaa
//
LOCUS       DAUVUY010000075         9355 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.075, whole genome shotgun sequence.
ACCESSION   DAUVUY010000075 DAUVUY010000000
VERSION     DAUVUY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.075"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(74..754)
                     /locus_tag="VOX58_001514"
     CDS             complement(74..754)
                     /locus_tag="VOX58_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HES5878733.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            928..1293
                     /locus_tag="VOX58_001515"
     CDS             928..1293
                     /locus_tag="VOX58_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HES5878734.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            1330..2349
                     /locus_tag="VOX58_001516"
     CDS             1330..2349
                     /locus_tag="VOX58_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HES5878735.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            2803..3123
                     /locus_tag="VOX58_001517"
     CDS             2803..3123
                     /locus_tag="VOX58_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878736.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            3113..5113
                     /locus_tag="VOX58_001518"
     CDS             3113..5113
                     /locus_tag="VOX58_001518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HES5878737.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEERHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKKHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVALWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            5115..5594
                     /locus_tag="VOX58_001519"
     CDS             5115..5594
                     /locus_tag="VOX58_001519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HES5878738.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            5662..6246
                     /locus_tag="VOX58_001520"
     CDS             5662..6246
                     /locus_tag="VOX58_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HES5878739.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            6262..7260
                     /locus_tag="VOX58_001521"
     CDS             6262..7260
                     /locus_tag="VOX58_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HES5878740.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTETQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            7257..7577
                     /locus_tag="VOX58_001522"
     CDS             7257..7577
                     /locus_tag="VOX58_001522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HES5878741.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            7778..>9355
                     /locus_tag="VOX58_001523"
     CDS             7778..>9355
                     /locus_tag="VOX58_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HES5878742.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGDFTVTDTVYEIKKEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     QSNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNI"
BASE COUNT         2783 a         1674 c         1986 g         2912 t
ORIGIN      
        1 gtcagtcatc ggtatatcag agattgtgat gcttatctga aatactttta actggcgttt
       61 tatggtgatt tggttattga tcggcttcaa tttttttaag gtagcggtaa acacttggtt
      121 cggaaataca tagaatatca gcgacttttg agacggctcc ttttagttga aatactcctt
      181 tttcataaag cttggcaaca atgtctattt tgacatcttg gctaagatgt acattgtgtc
      241 tcagtaacga gggatcaata atttgaccga taatatcttg gatgttagag gttaaaattt
      301 caacagcttc ttctggctgg ggtgtgactg ttttaggttc tttagcagtt gggatgaaat
      361 gttctaaatt gagattgaca agttttaaga ggtccctagc tactccttga taggctgaaa
      421 tatctaagtt aatacatagc attccctcga gattaccctt tttatcttta ataaaaaaag
      481 tagatccccg aacttctttg tgctgaggac taacgattgc tttgtaattg gtcacaaaat
      541 ctttttcctt atagacttta ttggtaatca aatcaagagc aaaggtggtg agaggagact
      601 gtttagaccg tccactgata tgactattaa cgagttctcc aatatagata tcatttttat
      661 caatcacatg taaaataatc tcatagttat cacctaagac atcgtgaaga aatgtgataa
      721 ccgttttcca gtagtttagc gtttctttat ccatgtcaaa cttcctatat ctatcttctt
      781 atttacaaat atagtaatac attttagact aaaaaacaat tgccaatcac cttaataata
      841 attttttatc aaaaagagaa tttattattg actgtgaatg cggtttcctg taaaatttaa
      901 ctatattatc aatgaggagg ttcgtatatg aagagctacc cagaaccaat gggtccttat
      961 tcaacttata cgattgaagg acattttcta tacaccgcag gccaattacc ccttaatcca
     1021 gtgacaggtc aattgtcaga tggttttgag gcacagtgtc gccaggtttt tgttaattta
     1081 caatctattt tggcagagca aaagttggac ttgaatcata tctataagct aaatgtgtat
     1141 ttaacagatg tgaccaatgt ggaaatatta aatcatgtca tgactgattt gtttgaagag
     1201 ccctaccctg ttcggacagc ggttcaagtg tcggcgcttc ctttgcaagc tttgattgaa
     1261 gtcgaagctg ttgccagagt gaagcagtta tagtgtctta tgtaatgaga tagattggtg
     1321 gaatagatta tggatattat tattggaaca agtcttttga ttcttgtttt agctattttt
     1381 agcttgttta attataaggc tcctcatggt gccaaggcaa tgggagcttt ggcttcggca
     1441 gcttgtgcct cttttttagt tgaagccttt caagattctt tttttggtaa ggttttaggc
     1501 tttcagtttc taagtgaagt aggcggagca aatggctcat tatcaggggt agctgctgct
     1561 atcttggttg ccattgcaat tggtgtgtct ccaggttatg ccgttttgat tggtttgtca
     1621 gtttcaggaa caggtattat cccaggtttt gtggctgggt atgtggtatc ttttttaatc
     1681 aagtggatgg aaaaaaatat tcctggtggg cttgatttaa tctcgattat tattgtcggt
     1741 gcaccattaa ctcgtttctt ggcgcagtta attacgccag tgatcaatag tactttattg
     1801 actattggag acatcctgac ctcaagtgct aatagcaacc ctattatcat gggaatgata
     1861 ctaggcggta cgattgttgt tgtagcaaca gctccgctgt catcaatggc tttgacagcc
     1921 atgcttggtc tgacaggtat tccgatggct ataggtgcct tatctgtctt tggctcttct
     1981 tttatgaatg gtgttctttt ttaccgatta aaattgggtg aaagaaaaga caatattgct
     2041 tttgctattg aaccactaac gcaggcagat gtgacgtcag ccaatcccat tccgatttat
     2101 gtgactaatt ttgttggtgg tgctgcatgt ggtgttttga tcgccctaat gaaattggtt
     2161 aatgatacgc cagggacagc tactccgatt gccggttttg cggtgatgtt tgcctataat
     2221 cctgtcgcta aagtgttgat aacagccctt ggatgtatta ttatcagctt aattgtgggt
     2281 tatattggag gttctgtctt taaaaattac cgtttagtaa caaaacaaga attacaagcg
     2341 agaaactaat aaacatcttt ttgacaggac ttttaacaag atagcagtta tactgttatc
     2401 acattggctt ggctattggc tggtaaaggt tccttaccgt caactagatc aaggttacta
     2461 aatgcctaaa gaagtataag ataacacacg aaagacagca atacaaagct ttagagttct
     2521 gtgtcatctt actgctttga caggttttta gctaaccttt ttctgaacta agccattgtt
     2581 tttatggata gaattgaaag cgcttaactt aaagatgctg ttcgcttaat caagcaatta
     2641 tcctatctca aaaaagctgt tagtagatag gttagagatt tagctctagc ttatagtcgc
     2701 tggttgatta acccccaaaa gtagcctttc ttgtagcttt gtttttaagt acctagtgag
     2761 tcaaaaaatt agcaatagat aaagataagg agttgatatg tgatgaatac cctattgtca
     2821 ctaatgaata gtatcgaaca agaggctaga acaatttatg acagttacga tgctaagaaa
     2881 aaagctctag ctgacaaaaa agaagaagag ttaaagcagc tgtttcttga gtacgaacaa
     2941 gaaactcagc tactattaga agctaaacgt gaggtatttt ttgaagctaa tcgtcaagaa
     3001 caaaaagctt caaaaaagag gttagcgacg caacagctag ctcttcatgc cattttagaa
     3061 gagcataagc taaccgttgt tagggagtta gtggataagg tggtggagac atatggccat
     3121 tagtcaaatg aaaaagctgg caatggtttt tgaaaaagat tatcttgatc ttgttttaaa
     3181 aacacttcaa caatctcaat tagttgaagt tcgtgacatg aagcaactaa aacattggca
     3241 agatgctttt aacaaaggca acgtcaagtt gccacaaatc gtccaatacg atctgacgca
     3301 tcaaaaacct ctactagatg atgaagcttt acaatacctt ttacaaagcc aacaagagtt
     3361 agaaaacggc cttgctagct tatcagcgtt tttaccacca ataggaaagt taacagcctt
     3421 gagacaaaaa acgccatctc tttcgtttaa acagttcgaa gaacgccatc gacaacaagc
     3481 agctcaaacg gctttgaaaa tgatgtcaca aaagatagag cgactagagc agctgcaaaa
     3541 caaaatagac cagctaacag aatactgtca ggagttagaa aagtggcgtt cattgactgt
     3601 tttgcctcag gacttagcgc aatttcattt tttgtcggca agagttggaa cgattcctag
     3661 tacagcaaat gatcattttt atcatcagtt aaagaagcac aaaggtcttt ttattgagga
     3721 agtataccat acagaatttg agtacggcct tgtcttattt tggcaagcac aagatacgat
     3781 tcatttacag aaatatcagt ttaaaccctt attatataaa gaacagctac tgccatcaga
     3841 acaacttcgt ataaacaaag agttactaac gaattggcta gctgaaaaag atagtctact
     3901 aaaagagctt aggcagtctc aaaagatact ggcacagtta caggttgaaa tagactatgt
     3961 ccttagtcag tatcaacggc agcaaactaa aaagcaatta ctcggtacaa gacatctaat
     4021 agccttagaa gggtggatag aagctgattc ggttaaccaa ttaaaagggc taatgactaa
     4081 gacactaggt gatatgtttt atctggatag ttacgatgtc acgccagacg attgggaaga
     4141 tgttcccatt aagttacgta atcaccgcta cattgcaccg tttgaattgg ttacagaaat
     4201 gtacgctttg ccaaaatatc aagaaaaaga tccgacacct tttttagctc ctttatacct
     4261 tacttttttt ggcatgatgg tagctgattt gggctatggt ttgttgctat atgctgtgac
     4321 actagctgca ttggtctttt ttaatctcca aaaaaccagt aaacggttag tgaccttctt
     4381 taatatttta gctatttcag tagccctatg ggggcttatt tacggttctt tctttggttt
     4441 tgacttacct gtggcattat tatcaactaa aacggatgtg attactattt tggttgtttc
     4501 tttgctattt gggtttgtta cacttatatt tgggcttttg ttaggagcat ggcaacaagt
     4561 aaggatgaaa gcatatgcta cggcctatac gtcaagtcta gcttggactt ttattttatt
     4621 aggcctgctg ttatttattc ttggtaagaa tgtatctggc cttgcttact tatcggttat
     4681 tggtaagtgg cttgcattgg gcaatgcctt tgggattttg gttgtttctt tactaaaaag
     4741 caaaagcctt ttaggtctag gtagtggatt atataattta tatggcatta gctcttactt
     4801 aagtgatttg gttagcttta ctcgtctaat ggcattaggg ctttctggag ctagtattgg
     4861 agcggctttt aacatgattg ttgggatttt tccaccagtc acgcgcttta cagtaggtat
     4921 atttattttt attcttttgc atgcgatcaa tattttctta tcaatgcttt caggatatgt
     4981 gcatggtgct cgtcttattt tcgtagagtt ttttggaaaa ttttatgaag gtggaggaaa
     5041 agctttcaat cctttaaagt tagctgataa ttatgtcaat gtcaatgaag aaacagattt
     5101 ggaggacaat taacatggaa catttagcac attattttac agcacatggt ggcgttttct
     5161 tcgcagcctt aggaattgtt ttagccgtag ctcttagtgg catgggctca gcttatggtg
     5221 tcggtaaagg tggtcaagcg gcagctgcgc tcttaaaaga agaacccgaa aaatttacgt
     5281 ctgcgcttat cttgcaactg ttacccggta gtcaaggaat ctacggcttt gctattggta
     5341 ttttaatttg gatgaaactg acaccagaac tttcagttaa ccaaggatta gcttactttt
     5401 tggtatctct tccaattgct attgttggtt atttttcggc caagcaccaa ggcaatgttt
     5461 ctgtagctgg tatgcaaatc ttggctaagc gccctaaaga ttttatgaag ggtgtcattt
     5521 tagcagctat ggttgaaacc tatgctattc ttgcctttgt cgtctccttt attctcttaa
     5581 ataaaattgg ataaggtatg gtccttatct agattttaaa aaacggtgac accactaaca
     5641 ctatcaaaag ggggaaatta ggtgaatgat ataacacagt taagacaaaa cgttcttgaa
     5701 aaagcacatc aagaaggtca acagtgccta aagatagcga cagatagtct tgatactgac
     5761 tttaaagagc gtcaacagca aggactacac gatttaaaag caaagcgtca aaaagagtta
     5821 aaagctcttg aacagcaatt tcaggttgcg cagcagcaac tcaaaaatca agaaagacaa
     5881 gctctactag cattaaaaca agacagtata aaggagctat ttgaagcttc tttggagaag
     5941 atgacgaact tttctaagga agaagaattg gcttttttga agcaagtctt gtctaaatat
     6001 ccagaacagc cattgcaagt tacttttggc gaaaaaacag ggcaaaagtt tagttcttat
     6061 gattgtgctg agctgagatt agcgtttcct caattaagct ataatcaaga gctaattcct
     6121 caggaagcag gatttttagt cagtttagac caagtcgatg acaattattt ataccgttac
     6181 cttttagaat cagttcttaa agaagaaagc tcccgcatca ttgatatgct tttttcagag
     6241 atataggagg tttgactagc gatgccaaca tttctagaat taaacactac tatcagtgtc
     6301 aaagaaaaag agttattgac taaagagcaa tttgataagc tattgcaggc tcccaataca
     6361 acaaccttag ctcgactgtt gcaccagtca gtctatcacc taactgttga cgatctcaac
     6421 gatttggatc ggctagaatc tatcttaatg gctgagctaa ccaagaccta tcgctgggct
     6481 tttgcagaaa cccctcaacc tgacatagta caattattta ccttgcgtta cacctatcat
     6541 aatgtcaaag tgttgttaaa agctaaagct agtcaagcag atctgagtca cttattacta
     6601 ccaattggtg acaagccttt ggtggctttg gaacatttga ttaggacgat gacttctgat
     6661 gagtttccaa aagaggtggt aacagaaacc cagtccattt gggccgaata tcaagattat
     6721 caagacatac gcgtcttaga aattggtacc gacttagctt attttaaggc attgaaacaa
     6781 atagcacaaa gattagagga tcctgttttc caacaagctg ttcttattgt gattgatttg
     6841 tataatctga ttaccgtcag acgggccaaa tcgcaaaata aaccaataag tttcatgatg
     6901 cagttattat ctgatgaagc aagtagacca agtaaaacat ttatcacact tgaagatgat
     6961 aaggatctga tgacgtggtt tgaaaatgta acacctgata gctatatgac agccctaaaa
     7021 ccttattcag agaaacttcg ccaaggcact cttcaaacaa ctgagttaga ataccttgtt
     7081 gatgaatgcc tttaccattt atttgcgaag gccaaatatc aagtcgatgg accttatgtg
     7141 cttgctcgtt ttttgctcgc taaatcgttt gaagtaaaaa atctacggct tttagcggct
     7201 gctttagcta atgatttacc caaagaacgt gtcatagaaa ggatgagacc aatagcatga
     7261 ccaaaacatt gtataaagtc ggggtaattg gtaacagaga tgttatttta ccttttcaga
     7321 tgattgggtt tcaaactttt ccagtaataa aaccacaaga cgctattaac cagttgcgtc
     7381 aactagcaat ggaagacttt ggtattattt atatcacaga agatattgct gcagctattc
     7441 cagaagccct cactcactat gacaatcaag ttctaccagc agtcatacca ttaccaactc
     7501 atcaaggagc gcaagggata ggcctgtcac gcatacaagc catggttgaa aaggctgtcg
     7561 gtcaaaatat tttataacac taagacgtta ggcaaagagg acttatactc tgtttgaggc
     7621 ttatgagtag ctaactgatg aaatgtaagg acaggaaaat tggtgaagtt agcagctgaa
     7681 cgcataggat agtttgctga ctattcaaaa tagtcacatt aaaccttgac agtatagatt
     7741 agttagtatg atttggaaaa aataaaggag gaaaaaggtg aaccaaggaa aaataataac
     7801 cgtttcggga cctcttgttg tggcttctgg gatgcaagaa gctaatattc aagatatttg
     7861 tcgtgtggga catcttggct tagtcggaga aattattgaa atgcgccgcg atcaagcgtc
     7921 tattcaggtt tatgaggaaa catcagggat cggtccagga gaaccagtag tgactactgg
     7981 ttgtcctttg tcggtcgagt taggcccagg cctgatttca gaaatgtttg acggtattca
     8041 gcgaccgctt gatcgttttc aaaaagcaac ggacagcgac tttttaatcc gtggtgtggc
     8101 tatcccaagt cttgatcgaa aggctaagtg ggcatttatt cccaagctaa gtgttggtca
     8161 agaagtagtt gcaggtgata ttttaggaac tgtgcaagaa acagctgtca ttgagcatcg
     8221 tatcatggtt ccttataaag tttcagggac cttggtggct attcatgcag gtgacttcac
     8281 agtaacagat acagtttatg aaattaagaa ggaagacggt tccatttacc aaggtagcct
     8341 catgcagact tggccagttc gtcaaagtcg ccctgttgct caaaagctta tcccagtcga
     8401 acctttggtt acaggtcaac gggttattga cacctttttc cctgttacaa aaggtggtgc
     8461 cgctgctgtt cctggaccat ttggggcagg aaaaacagtt gtgcagcatc aaatagctaa
     8521 atttgccaac gttgatattg ttatttatgt cggttgtggg gaacgcggca acgagatgac
     8581 cgacgttttg aatgagtttc cagagttaat tgacccaaat acaggccagt ccattatgga
     8641 gcgcacggtg ttaattgcaa acacctctaa tatgccagta gcagcgcgtg aagcgtcgat
     8701 ttacacaggc attaccattg ccgaatattt ccgtgatatg ggctattctg tggctatcat
     8761 ggcagactcg acatcacgtt gggcagaagc tctgcgcgag atgtcaggac gcctacaaga
     8821 aatgcctggt gatgaaggct acccggctta cttagggagt cgtattgccg aatattatga
     8881 acgggctggt cgtgttcgga ccttgggaag tcaagaacgt gagggaacca ttacagccat
     8941 cggcgcggtt tctcctcctg gaggggatat ttcagagcct gtcactcaaa acacccttcg
     9001 gattgtcaaa gttttctggg ggctcgacgc gcctcttgcg caacggcgtc acttcccagc
     9061 gattaactgg ctgacgtctt attcattgta tcaagatgat gtaggaagct atattgaccg
     9121 taaacagcaa tctaattggt ccaacaaggt aactcgtgcc atggctattt tgcagcgtga
     9181 agccagtcta gaagaaattg tacgcttggt ggggcttgat tcactgtctg aacaagatcg
     9241 tttgaccatg gctgttgccc ggcaaattcg ggaggattat ctccagcaaa atgcctttga
     9301 ttcggtggat acctttactt cctttccgaa acaagaggcc atgctaacca atatt
//
LOCUS       DAUVUY010000076         9219 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.076, whole genome shotgun sequence.
ACCESSION   DAUVUY010000076 DAUVUY010000000
VERSION     DAUVUY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.076"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..534)
                     /locus_tag="VOX58_001524"
     CDS             complement(<1..534)
                     /locus_tag="VOX58_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HES5878743.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGV"
     gene            complement(572..1894)
                     /gene="rsmB"
                     /locus_tag="VOX58_001525"
     CDS             complement(572..1894)
                     /gene="rsmB"
                     /locus_tag="VOX58_001525"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HES5878744.1"
                     /translation="MADNWKISIRGKALLTLEHIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSDRSVAIMESLFVRSKASIRVTDPLKLEEAAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            complement(1884..2819)
                     /locus_tag="VOX58_001526"
     CDS             complement(1884..2819)
                     /locus_tag="VOX58_001526"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HES5878745.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMELGADGIITAAFGQFLPTILLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSD
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIAAGQGA
                     LSLLVVQPAGKPKMAIADFLNGLGRKLEVGDFIGR"
     gene            complement(2881..5265)
                     /locus_tag="VOX58_001527"
     CDS             complement(2881..5265)
                     /locus_tag="VOX58_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HES5878746.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKGIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTKRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKQQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            complement(5330..5647)
                     /locus_tag="VOX58_001528"
     CDS             complement(5330..5647)
                     /locus_tag="VOX58_001528"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HES5878747.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            complement(5663..6298)
                     /gene="gmk"
                     /locus_tag="VOX58_001529"
     CDS             complement(5663..6298)
                     /gene="gmk"
                     /locus_tag="VOX58_001529"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HES5878748.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            complement(6408..8015)
                     /locus_tag="VOX58_001530"
     CDS             complement(6408..8015)
                     /locus_tag="VOX58_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HES5878749.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            complement(8145..9041)
                     /locus_tag="VOX58_001531"
     CDS             complement(8145..9041)
                     /locus_tag="VOX58_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HES5878750.1"
                     /translation="MDIKQIRYFIAIVENHFNLSQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSISIGIPPL
                     ILSVVFSEVMPKLILENPGIQFNVKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYEIQRSELSVCLSPRHRLASKKVIQWEDLTDEQLALFDPSFMVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQFKDIKCILMERPISWRVVLTRL
                     RKKSYSEIEAYIMDDLLQSFFK"
BASE COUNT         2694 a         1931 c         1610 g         2984 t
ORIGIN      
        1 cactcctaaa tcaggctcta ctggggaagc ttgaccaata gattgagtga taatatttct
       61 ttgtggatgg ctagcagctt cttcttctgt gatctgtcct gccttaacca actcattcac
      121 taaagaatga tcactggtca ataaggtata atgcccatca tggacaagac caatccgtga
      181 atcaccaata tgagcataaa tggcgctaga ttcaactaaa gctactgctt caacagtcgt
      241 tcccattcct ttaaagtctt ctgattggcc tagatcataa atccgttgat tttctgactg
      301 gatagtttca aataaccaat ctctaatctg acttaactct gtgaagtctg tttttaccca
      361 ttctcttccc aggtctgtga ctgtcatctc actggcaata tttccagctc tgtggccacc
      421 cataccgtct gctaaaatca ctaaagtgat gcctttttta ttatcaaact tattgataaa
      481 atcttgattg tttgagcgtt tttgcccaat gtctgttttt aatgaaatct tcatacgttt
      541 tttctgactc agtcagtttc ctcctaaaat actacaagac tcgtctgact tgtccaataa
      601 agaagccatc agtctggtat tgttctggtg tgataataag ataaccatcc ttgacaatat
      661 ctgcttgtgt atggttcagt tttacttgtt caaaattagg atgactttgt aaaaatgctt
      721 cgatgacttg acgattctcc tcatcaaaaa tagtgcaagt actgtatgtt attataccac
      781 ctttacgcaa ggtttgacaa acactagaca atatctctag ctggatagcc tgtagagcat
      841 tgaaatcttg actttcttta ttatatttga tatcgggttt tcgacgaatc aaaccaattc
      901 ctgagcaagg agcatccacc aaaatcttat caaagctatc cttttcaaaa tgacgatgaa
      961 cctctctggc atctagtttt cttgtctcaa tgttatcagc aactcctaag cggttagcat
     1021 tttccttcac caactctagt ttatgatcat agagatctaa ggcaataact ttacctgttt
     1081 ttaagtaaga tgctatatga gaggtcttgc cccctggagc gctacatgca tcaagaatta
     1141 tgtcgtcacc gtcaatgttt aaagtaggag ctacaagctg actgctttca tcttgaatag
     1201 tgatgtcccc atttgtgaaa taatcactgg ctgcaaaatg acctgacgct ttggttaaac
     1261 cagttgctga taaaagggaa cgttctgcat ctaaagcctc agcagcttcc tctagcttca
     1321 aagggtctgt cacgcgaata ctagccttac tacgcacaaa taaactttcc atgatggcca
     1381 ctgatctgtc agaaccaaat tggtcttcta actttttaac caaccaaaca ggcagtgaat
     1441 acttgaccga ataatactta tttcgacgct taattgtttc catatctggg agaggatggc
     1501 tggtgaattg acgtaaaata gcattgacaa acttctctgc acctttttta ttgccacgat
     1561 ttttggcaat accaaccgca tcgttaacaa tggcatgcgc tggtaactta tctaaataaa
     1621 tcagttgata aagactgagc ataaggagat aataaaccca tttgtccaat ttatctcggt
     1681 ctttgacata atgggctaaa taccattcca agctaatttt acgtgaaacg gtaccatata
     1741 caatctcagt taatagagct ctgtccttag cagataaagc tttattgctt agctgctgat
     1801 taagcgctat gtttgtgtaa gccccctgat caaaaatatg ttctaaggtt agaagtgctt
     1861 ttcctcgaat acttattttc caattatctg ccaatgaaat cacccacttc aagttttcta
     1921 ccaaggccat ttaagaaatc tgcaatagcc attttaggtt tccccgcagg ttgcacaacc
     1981 agcaatgaca gggctccttg accagcagct attaccaaag attttttggt tttgacaata
     2041 acttgtccag gtaatccttc tccttcagct aattgagctt cgtaaatttt taaacgttgc
     2101 ccctctaaaa acgtatgcgc taccggccaa gggttcatac ctcgaatatg attgaacact
     2161 tcttgatcag acattgtcca atctaatttt tcttgttctg gagagatatt aggggaaaaa
     2221 gtagcttggc tgtgatcttg aggaataggt tttaattctc cagaaagata agctggcaaa
     2281 ctatccaaaa gtaagtctcg accaatgatg gctaatttct caaaaagagt acctacatta
     2341 tcggtttcaa gaattggagt gcttgcttta gcgaccatgt cacctgcatc catttctttt
     2401 atcatttcca taatagtaac gccagcctct ttatctccat tcataatagc ataatggata
     2461 ggtgctccac ctcgatattt aggtaacaat gatgcatgta cattaatagc aaatgaaact
     2521 gaatcaagta atattgttgg taaaaattgg ccaaatgctg ctgtaataat accatctgca
     2581 ccaagttcca taatctcaat gagttcttga gagcctgata atttttccgg ctgatagatt
     2641 gatattccat gctcaagagc taattgcttt acaggtgtta ccttaatatc ttttttgcgt
     2701 ccaacagctc gatcaggctg agtaaccact cctaaaatct cataggcagg attatctagc
     2761 aagcccttca atacagttgc tgaaaattgg ggagttccca taaataataa ttttatcatg
     2821 actttcctct ttgtattttt attatatcat aactgatcat cctagattta gtgatttacc
     2881 ttataaaaat tgttgcggtt cacaatcaat aattagtttt aaatgacgat tatctgcttc
     2941 ttgactccaa tcaagaatac gattgagcgt ctcttctagg ttatcttcaa aacggtattt
     3001 taacaaaatt tgatagtggt ataaattgtg tgttctagca attggcttag gagtcggtcc
     3061 taatatttta atattatccg ataagtgact ctttagcagt tctgtaactt gataagcttt
     3121 ttttacaacc gatgcttcta aacgatgcga aagcgtaata cccactgtaa agtagtaagg
     3181 gggataagcc atctgacggc gaatcgccat ttcataacgg taaaaggctt caaaatcttg
     3241 ttgtttagct aattgaatag catagtgatc gggattataa gtttgaatca agacttcccc
     3301 tggcttatgg gcacggcctg cccgaccagc aacctgtgtt aacaattgaa acgttttttc
     3361 tgacgctcga aaatctggca aatttaagga ggtatctgca tttagtaccc caactaaagt
     3421 cacatttgga aaatccagtc ctttggcaat catttgcgtc cctaacaaaa tatctgcttc
     3481 ctggcgacca aaactatcta gtatggtttt gtgacttctt ttttttcgtg tggtgtcaac
     3541 atccattctc aagatcctag cttctgggat aaccccttgc aattcatcaa aagctttttg
     3601 agttccagtg ccataatagc ggatactgtt gctatgacac tcaggacaag taattgggat
     3661 aggtttttga aacccacagt aatggcaatt catagtttta gtatccatgt gaagggttaa
     3721 agagatatca cagttgggac atttgtccac ataaccacag tcccgacaca tcacaaaact
     3781 agaataacca cgtctattta acattaagac aacttgttct tttttaacca aacgttcttt
     3841 aatcttatca atcaaatatg gggtaaaatt actaactgct tgctgtccaa tataatcacg
     3901 aaaatcaaca atcgttactt ctggaatctt agcaagaggg tttgctcttt tcgttaattg
     3961 aataaaatga tagacaccct tactagccct tgctcggctt tcaatacttg gcgtagcaga
     4021 gcccataacc acaactgctt gatggtgttt agctctgagc agggcaacct ctctggcatg
     4081 gtatctaggg ttgctttctt gtttataggt tgattcatgc tcctcatcaa taataattgc
     4141 accaatccgc tctagtggag agaaaatagc agagcgggca ccaaccacaa ccttagcctg
     4201 tcctgttttt atcttgcgcc actcatcaaa cttttcacca tctgataagc ctgaatgcat
     4261 aatagccact tgctttccga aacgggaaat aaaacgagag gtcatctgag gagtcaacga
     4321 aatttctggt actaaaacaa tggctgtctt atcctgtttt aaaaccgctt cgatgatatg
     4381 aagataaact tctgtcttac ccgaacctgt aataccttca atcaaaaaag gtttactcgg
     4441 tttcccaatt tgctcaacaa tttcagtaac agctgatgcc tgctgttcat ttaaggttaa
     4501 aaaagaagat ggtttgatct ggtcaaaata gggctcactg cgatcaattt gacgttcgtc
     4561 aatacgaatt aagtgatttg tcacaaagta agccaccaca tctctagaaa aggcttgata
     4621 aagcgttgca agtttagctt ccttcgtatg ggttagcaga tagtctttta ataattgacg
     4681 ctttttggca cgactagaga tgggatgtac cgctaaggcg tctaagtcga cgtgataata
     4741 tttttctgtc tttaggtttt tcttgtcttt ggctaaataa ctaacagtaa tatgcccagc
     4801 ttgtatgccc ttaagagcta ttttttcctt ctcgcggtct aaggtagaat ataagacaga
     4861 tggttttcct tcaaaaagaa agtccctatc aggctttttt agacctgact ctggccttag
     4921 cactttatca tagttagagt taagaagatt aggaatcatt gcttttaaga gcgtgatttt
     4981 ataagaaaaa actgtttttc gaagctgatc agcaagggtc aattgttctt ggtttaggac
     5041 tggctcagga tctaaaacgg tctgaatcag tttaagagag cttgaatctt cctggccaaa
     5101 accaataaca aagccttgca acaaacgatt cccttttcca aaaggaacat gcacacgaga
     5161 accaagctgc acgagagaaa ctaactcttt tggaatacca taagaaaatg gcttgtccgt
     5221 ttgcatcaat ggaatatcta caatcacatg agctactttt tccatttgct tctccttttc
     5281 tgtcatggac atcgtactaa atatgaaaaa atctaagatt tctgaaacct tagatttttt
     5341 ctccttcttc ttctttttct ttggcaattt gttctttgat tttacgttcc tcttcctctt
     5401 tggccaagcg ttctgcctca atcttagcac ggacagcttc acgtttagct gaaggatctg
     5461 ggtgaatcac aacgtttcct gattcaattt cttcaagtgc ctgaagtgtt gacttcactg
     5521 atttaaattc ttgagttgga gtggcaccag cttctagttc atgggcacgt ttggcttgta
     5581 aaatcacaag tgaatactta gatggtactt tatctaataa ggtatcaatg gaaggtttta
     5641 acatcataat gcttactctt ttctatttag ctttaaaggg attttttgtt attttaatca
     5701 ttttatcata acgaccaatc acgcgctcca cacgaaagtg ttctgtctca atgatacgct
     5761 taacccgctc agctgccaag gcaacttcat cattaacaac tgcataatcg tattcgcgca
     5821 taagggcaat ttcttctttt gctctttcaa tacgttgagc tatgacttct tgactatctg
     5881 taccacgacc aaccaggcgg tcttctaatt catctaaatc tggtggtgtt aaaaaaacaa
     5941 aaacaccatc tggtactttt gacttaacct gaagagctcc ttgaacttca atttctaaaa
     6001 agacatcaat tcccttatca agagtctcat taacataagt taaaggggtg ccatagtaat
     6061 taccaacata ttcagcatac tctagcattt gaccagtttt aatgagttct tcaaattctt
     6121 cacgtgtgcg gaagaaataa tcaaccccat caacctcgcc cggtctttgc ggacgagtcg
     6181 tcattgaaac cgaatattca aatttatgat ctggcgttga aaaaatttct tgtcttaccg
     6241 ttccttttcc gacacctgaa gggccagaaa agacgattaa caaaccgcgt tcagacatta
     6301 tattctcctt attagttgct ttttaatagt ttatcaaaaa ggctagtctt tttcaagaac
     6361 attttcaaag gcaagtcata aagaaaaaga ggataattcc tctttttcta cttggcataa
     6421 tcaaccgctc tcatctcacg aataacagtt actttaatat ttcctgggta atctagattg
     6481 ttttcaattt tttctcttac tttatgcgac aaaatgacaa cctgatcatc tgaaattttt
     6541 tcaggttgaa ccataatacg aatctcacgt ccagcttgta gagcaaaact attttgtacc
     6601 ccatcaaaac ttgtcgcgat ttcttctaaa tcacgaagac gcttgatgta attctccatt
     6661 gactcattac gagcgcctgg gcgagccgaa ctgagagcgt ctgctgcagc tactagcaca
     6721 gcgatcacag aatctggctc cacatctccg tggtggctag caatagtgtt gacaacaact
     6781 ggatgttctt tgtatttacg tgcaaattcc attccaatct caacgtgact accttcaacc
     6841 tcacggtcaa tagctttacc catatcatgc aagaaaccag cgcggcgggc aagggcaaca
     6901 ttttcaccca gttcaccagc taaaatacca gctaacttac caacctcaac agagtgacgt
     6961 aggacatttt gaccaaacga ggtacggaat tgcaggcgtc ccatgatttt aatcaaatca
     7021 ggatgaaggt ttggtgcacc aatctcatag gctgcagctt caccgtactc acgaatacga
     7081 ttatccattt caagacgatt tttctcaacc aattcctcga tacgagctgg atggatgcga
     7141 ccatcagcaa tcagagattc caaggtcata cgagcaattt cacgtcgaat aggatcaaat
     7201 cctgataaga taacaacttc aggagtatcg tcaataataa cgtcaatgcc agtcaagctc
     7261 tctaaagtac gaatattacg gccttcacgt ccaataattc ggcccttcat gttgtcgtct
     7321 gggagatgga cactggtaat agtttgttca gtcacatact caccagcaag gcgttgcatg
     7381 gcttgcgcta acaagtcctt ggctgtttta actgttcggt ccttgatgtc acgttcggca
     7441 tctcgaatgc gcgtcgcaat ttcatgggtc agtttgtttt ccgtctccat taaaatcact
     7501 tcacgcgctt ctgcaatcgt catcgcagca actttttcca gttctgcttt tttctcctct
     7561 tcaagttttt ccacttgaag ttgccgctca tcaatgtgtt tagatttatc ggtcagactt
     7621 tgttctttac tatctagtac cttttcttta cttgataggt tttcatcttt acggtcaaga
     7681 gttaaggagc gctccgctaa gcgtgtctcg agttgtttaa gctcttgtct ttcagactta
     7741 aattcttgtt caatctcttc acgatatttt cttgcctctt cttttgcttc taaaagtaat
     7801 tctttacgat ttgctttact ttcacgttta gctgtttttt tgatgtgttc agcatctact
     7861 tctgctttgc cacgaatatc aacagcttct tgttcagcgt ttaaaagagt caactctgca
     7921 gcttccttcg cagacttgag tctaatcgaa ataagtgcat aacctaatat taaaccaatg
     7981 agggcagaaa caattaataa aataatatta accatgtttg tacctcaata tattctagat
     8041 ttcattaata gaaagccatc tcttagttta tcataaattg agcgtttaca caattccaat
     8101 tcatttttaa aaaaattaac atttgggcca agtagttgag aaccctactt aaaaaaagac
     8161 tgtagcaagt cgtccatgat gtaggcttct atttcagaat aacttttttt ccggaggcgt
     8221 gttaagacaa cacgccaaga aatgggacgt tccattagaa tacacttaat gtccttgaat
     8281 tggtataatt ctgtaatagg tttgggacaa atagttaaga cgttgtgatt aatcttggta
     8341 gaattaagca taaaatccca ggaagaggac gtcaaaataa tattaggtct gacttggtga
     8401 cgctcgcaag cttcaagcac taaatggtga accatgaaac taggatcaaa tagggcaagt
     8461 tgttcatctg ttaaatcttc ccactgaata acttttttgg aagctaaacg atggcgtgga
     8521 gacaggcata cagataattc tgatctctgg atttcataag tctcaaccaa attatcagcg
     8581 atgcctgttg gggataacaa gactgcgacg tcaacatttc ccacgagtag ttcatttttt
     8641 agttggtaag caccaatctc cttaacattg aattgaatcc ccggattttc taaaatcagt
     8701 tttggcatta cttctgagaa aacaactgat aaaataagag gaggaatacc aatactgata
     8761 cttcccttta accctttact gtgatcatgc aatttcaaaa acatgtcatc atagagactg
     8821 agaacctttt gagcgtcctt ataataatta tcaccaagat aggttaaccc aataatacga
     8881 cctctttttc gtttgaagag cttaacattt tctctttttt caaaatcgtt aatcatcata
     8941 ctcagcgtcg gttgagaaac atacaacagt tccgccgcct ggctcaagtt aaaatgattt
     9001 tcaacgattg cgataaaata acgaatttgt ttaatatcca aataggattc tccttgtctt
     9061 atcaattact ttttccggta taattaccta tattatgaca caagtagatg ttaaatgcta
     9121 ttgccaactc tatcaaaaaa aattatatgc ttataaaaat tctatatttt tatttttagc
     9181 gttttcttgt tatcttagag acatggaaag cgctttcta
//
LOCUS       DAUVUY010000077         8954 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.077, whole genome shotgun sequence.
ACCESSION   DAUVUY010000077 DAUVUY010000000
VERSION     DAUVUY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.077"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(22..177)
                     /locus_tag="VOX58_001532"
     CDS             complement(22..177)
                     /locus_tag="VOX58_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878751.1"
                     /translation="MTMLGYRLSAEVRGSAILPLLAGYLIRQAIAQVYSDLKTASWLF
                     GLASSVA"
     gene            303..560
                     /locus_tag="VOX58_001533"
     CDS             303..560
                     /locus_tag="VOX58_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HES5878752.1"
                     /translation="MTQNVCQQNITYTVKERFASYTLEAQANQEVHLNLTLLANRFGT
                     SDRHLKHVLKQPIFQRIIERICFKTCRIADLDALTQLSVNN"
     gene            686..1300
                     /locus_tag="VOX58_001534"
     CDS             686..1300
                     /locus_tag="VOX58_001534"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HES5878753.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(1333..1875)
                     /locus_tag="VOX58_001535"
     CDS             complement(1333..1875)
                     /locus_tag="VOX58_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HES5878754.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANVPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEALLAAI"
     gene            complement(2006..2434)
                     /locus_tag="VOX58_001536"
     CDS             complement(2006..2434)
                     /locus_tag="VOX58_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HES5878755.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            complement(2544..6941)
                     /locus_tag="VOX58_001537"
     CDS             complement(2544..6941)
                     /locus_tag="VOX58_001537"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HES5878756.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRNSHLPNLVKQFEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHDRKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQDAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLAKYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRANKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVENTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMGILGNMPEDNQLSLFDDFF"
     gene            complement(7195..>8954)
                     /locus_tag="VOX58_001538"
     CDS             complement(7195..>8954)
                     /locus_tag="VOX58_001538"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HES5878757.1"
                     /translation="QVSAGIYAYLPLANRTIEKFKTIMREEFEKIGAVEMLAPALLTA
                     DLWRESGRYETYGEDLYKLKNRDNSDFILGPTHEETFTTLVRDAVKSYKQLPLNLYQI
                     QSKYRDEKRPRNGLLRTREFIMKDGYSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFK
                     GIIGDGGAMGGKDSQEFMAVTPARTDLDRWVVLDKSIASMDDIPKEVLEDIKAELAAW
                     MISGEDTIAYSTESSYAANLEMATNEYKPSSKVAAEDALAEVETPHCKTIDEVAAFLS
                     VDETQTIKTLLFVADNEPVVALLVGNDHINTVKLKNYLAADFLEPASEEEARAFFGAG
                     FGSLGPVNLAQGSRIVADRKVQNLTNAVAGANKDGFHVTGVNPGRDFQAEYVDIREVK
                     EGEMSPDGHGVLQFARGIEVGHIFKLGTRYSDSMGATILDENGRTVPIVMGCYGIGVS
                     RILSAVIEQHARLFVNKTPKGDYRYAWGINFPKELAPFDVHLITVNVKDQVAQDLTAK
                     LEADLMAKGYDVLTDDRNERVGSKFSDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDS
                     IEVNAENLIETLEILTKEH"
BASE COUNT         2595 a         2047 c         1736 g         2576 t
ORIGIN      
        1 agctctcaca tccaaaccac cttaagcaac agaactagca aggccaaata gccaagaggc
       61 tgtttttaaa tcagaataaa cctgtgcgat tgcttgccta ataagataac cagctaaaag
      121 aggtaagatg gctgatcccc gcacttcagc tgataagcgg taacctagca tagtcattca
      181 tttctttcca acaaagtgtt cactgataat attaattgct ctagtattaa caaacccaat
      241 ccctaatcca tgaacacagc ttattaaccg atgcgacctg cctttatgac ataggtcaag
      301 agttgacaca aaatgtttgc caacaaaaca ttacctatac cgtcaaagag cgctttgctt
      361 cctataccct tgaagcgcaa gccaaccaag aggttcatct aaaccttaca ttattagcaa
      421 accgttttgg aacaagcgat cgccatctta agcacgtact taagcagccc atttttcaga
      481 ggattatcga acgcatctgc ttcaaaacct gtcgtattgc cgatttagac gctctaactc
      541 aattaagtgt taataactag acctctgagg tatgcagccc attaacttat aatagcaatc
      601 actctgacca ctacctgact tttctttaaa aaagaaatga tttgtgatat agtaaatcca
      661 gttaaaaaaa agtaaaggag tagacatgtc tgcacaagat aaattaatta aacctagtca
      721 tctgattacc atggatgaca ttattcgtga agggaatcca actttaagag ctgtcgccaa
      781 agaagtgagc ttacccttgt gtgatgagga tatcctatta ggagaaaaga tgatgcaatt
      841 tttgaagcat tctcaagatc ctgtaatggc ggaaaaactt ggacttaggg ccggtgttgg
      901 tctagcagct cctcagattg atgtgtcaaa acgtatcatc gctgtgctcg ttcctaatct
      961 tcctgataaa gaaggaaatc ctcctaaaga agcctatagc tggcaagaag tcctgtataa
     1021 tcctaaaatc gtttctcatt ctgttcaaga tgcagccctt tctgatggcg aaggttgttt
     1081 atctgtcgat cgtgttgttg aagggtatgt ggttcgccat gctcgtgtta cagtagacta
     1141 ctatgataaa gaaggacaac agcatcgcat caaattaaag ggatacaacg ctattgtggt
     1201 tcaacatgaa attgaccata tcaatggtgt tttgttctac gatcggatca atgctaaaaa
     1261 cccctttgaa accaaagaag agctcttgat tttagactaa caaaaaaggc cctttagcct
     1321 ttttttgtta gtctaaatag ccgctaaaag agcctctgcc tgagatagca agtttgcttt
     1381 tgtctctttt gaaatctcaa gccttcctgt tccccaggca tcaggattca cagttgcttt
     1441 tgtaaactct cctgctactg aagttcggat aaacggcaat agtgctttaa actgatcaaa
     1501 tacttgatca tgcccgccat ttgcaactga agagaccgta accaccttac cgccaatagc
     1561 agatgggccc gtcggatcag acaaatcaag agcacgagac aaccagtcta gcaggttttt
     1621 aacagaacct ggaatagaga agttgtaaac tggtgtaaaa atccagatag catccgctga
     1681 ctgaacagct tgacgagcgt caacaactgg taaaggtaca ttagcttcga tatcttgatt
     1741 caaaacagga acgtctttcc aatttaagta agatactact gcttgatgtt ccagagcttt
     1801 ttgtgcttga gccgctaatt gatggttaaa agacccttca cgaagcgagc caacaataaa
     1861 taaaatatgt ttcatctctt tgtcctcttt ttttaaagat cgttttaaag ctttctaaaa
     1921 gtggtatttc attgatactt ctttttgaaa tttatcacta tgtagtaatt atatcttctc
     1981 taagaagagc tgtaaagaga tttgtttata attgtttgaa ctttttgaga agttcaatca
     2041 gacaaagttg ttctttttca gtcaaaacag caaaggcttc ttccacccgt gcgacgtgtt
     2101 taggcaaaac agcttcaatc aagcgagtac ccttatctgt taaggtaacc acataggctc
     2161 ttttatccgt tttgtctttg cacttgctaa tccagccatt acgttccata ttatttagta
     2221 caacagtcat attccccgaa gtcgctaaca aagaatcaat caagtgattg attcgcatac
     2281 agcctttagt atatagtact tcaagcacac tgaactgcgt ggctgttaaa tctgcctttt
     2341 taaaaatatc agccccaaaa gcatctagcg ttcgctgagc tttacgaaac accaccatgg
     2401 ctttcaaagc ggtgttctta tctaaatgac tcatttgatt tcctcgcttt atacggtatg
     2461 tcacaattta gtatcagaga gattagaaag tgatcttgtc cctcttcttt tatgactttg
     2521 catatgacta ccatcatgat aatctagaaa aagtcatcaa aaagacttaa ttgattatct
     2581 tctggcatat ttcctaaaat acccatctca tccattttct caacgagcgt tgacgatgcc
     2641 ccgcctcgtt tacgcaattc cattttagag aggaattcgc cttcttgacg agctttaacg
     2701 atttgcttgg ccacgttttc acccagacct tctaacgcta taaatggagg gataagggta
     2761 tctcctttga tttggaattc tatagcatca cttttgtaaa ggtctaattt gccaaactta
     2821 aagccgcgtt ctaacatttc gttgacaatc tccaaggttg taaagaggtc attttccaca
     2881 ttggtggctt cattattttt acgttttata gtaatatctt ccattcttgc tttaacagca
     2941 tctaagccac cactcatggt ttttaattca aaagccttcg cacgaataga gaaataagca
     3001 caataataca taatggggtg gtgcaccttg aaataagcca cccgaagggc catcaagaca
     3061 taggccgccg catgggcttt agggaacatg tacttgattt ttccacacga ttcaatgtac
     3121 cagtcgggca cattgttttc tcgcatggca tcaatatagc cattacgttc ttcctcagaa
     3181 attttcagcc agaggccctt acgcacacgc tccataatgg taaaggccat tttcggttct
     3241 aagcctgcgt gcatgaggta aaccatgatg tcgtcacgac aaccgataac ggtttttagg
     3301 gtcgcaatgc cttctttaat caaatcttgt gcattaccaa gccaaacatc ggttccatga
     3361 gatagtccag acaactgcaa aagctccgca aaagtggtcg gatgcgtctc attaaccatg
     3421 ccgcgaacaa agttggttcc aaattctgga atgcctagca taccagtcgg tgtcccaatt
     3481 tgttccgggg taacgcccaa aatctctgtc ccagaaaaga gagccataac acctggatca
     3541 tcagcaggga tagtaatagg atcaatgccc gataaatcct gaagtttacg aatcatggtc
     3601 ggatcatcat gccctaggat atcaagtttc aagacgtttt catcaatatc atggaagtta
     3661 aagtgagttg tctgccaaga agccgttaca tcatctgctg gatattgcac gggggtaaaa
     3721 tcataaacat ccatgtaatt aggaataaca acaatccccc cagggtgctg cccagtcgtt
     3781 cgtttcacac cagcagcacc tgctgctaga cgatccacct cagcatcacg atagaacttg
     3841 ccatagtcgc gttcatagcc tttgacaaat ccataagctg ttttttctgc tacggtacca
     3901 actgttccag cacgaaaggc gtattcgtca ccaaaaatat ctcggacatc caaatgggca
     3961 ctgggctggt catcaccaga gaagttcaaa tcaatatcgg gcaccttatc cccatcaaac
     4021 ccaagaaagg tctcaaaggg aatgtcttgc ccatcttttt gataaggggt gccacatttc
     4081 ggacagggtt tattaggcaa atcatagcca gatccaactg acccatctgt gataaattca
     4141 gaatgttggc aggacgggca aacgtagtga ggcggcatag gattaacctc agtaatccca
     4201 atcatggtgg cgacaaagct agaccctaca gatcccctag agccaaccag atagcctcgc
     4261 tcatttgacc ggttaacaag catttgggaa gcgagataaa tcacagcaaa accgttcccc
     4321 aagatagagg ttaactcttt ttcaatgcgt aaatcaataa tatctgggag aggattacca
     4381 taaatttcaa aggctttttg ataggttaat tcggcaaccg tctcttcggc cttgtcaata
     4441 tacggggtgt aaagatcgcc cttaaccact tccacttcct caatacggtc cgcaaaatcc
     4501 tgagtatttt ctacaaccac ttgataagcg aggtcttttc caagaaaggc aaactcatcc
     4561 agcatttcat tggttgttct aaagtgcgct ttaggtaggg gagcaggttg ggccccttcc
     4621 ccacggccga ttgttctatt aatcatggca ccctgaccga gactacgcac aataatttca
     4681 cggtaaatct cttcttcagg ctctaggtaa tgcacatttc cagtggcaag tacaggttta
     4741 ttagctcgtt tccctacttc aatgaggtca cgaatcacct gctcaatacc tgcttggtct
     4801 ttgattaatt cacggacaac caatggctgg taaatggctg gtggcatgat ttcgataaaa
     4861 tcataatact tagccaaatc aaccgccgca tcaattcctt tagtcagaac ggcatcaaaa
     4921 acctcgccgt cagaacaagc tgtaccaagt aacaaaccct ctctgtgagc atctaagacg
     4981 gttcttggaa tacgcggcac cccttcaaaa tatttaatat tggaaaggct gaccaactta
     5041 aacatatttt taaggccaac ctgattttgc acatagatag tcgcatgctt aatccgcgct
     5101 tttttgtaag aatcctcagc taccaaatct gtattgagtt gcaaaagatt tttgatgcca
     5161 tgcttttctc tggcatctct tagaaaaata aacaaaagac gtcctgtggc ttccgcgtcg
     5221 taattggcca tatgatggtg gtctagactc acttggaaac gcttggtgag cggtcccaaa
     5281 ccgtgacgct tgtactcagg atacaagttt ctagcaaatt ctaaggtatc aatcacaggc
     5341 tgtgtgattt tgggcaagtc gtggcgttca taattggcgt tcataaagcc cacgtcaaaa
     5401 ctggcgttgt gggcaaccaa gatactatct ttgcaaaagt cctgaaaagc ttttaggacg
     5461 gtaaccaatg gcttggcgtc ctgcaaatgc ttatcggtaa tccctgtcaa ttcggtggta
     5521 aaggcagaaa gaggatgccc aggatcaatg aattcatcaa actgctctac aatattgcct
     5581 ttaaacattt tggaagccgc aatctgaatc aggtcattat tcatagcaga tagacctgtg
     5641 gtttccacgt caaagaccac ataggtagct tcgtgcaaat ccatatcaac aggatcataa
     5701 gaaataggca ccttgtcctc aacaatattg gcttctaggc caaaaatagc cttaattcca
     5761 gctttgcgag ccctatggta gccatgagga aaactttgca cattagcatg gtcggtaata
     5821 gcaaccgcct tgtgtcccca cttggctgcc gtatcaatca agctttctac tgtcggtaag
     5881 gcgtccatgg tagacatatt ggtgtgggca tgaagttcga cccgcttttg cccttctggc
     5941 atcaggtctt tgcggtcatg atggacaatt tctttgacct gctgcacatt catggtgaga
     6001 ctcttcgtaa aaggattggt ctcaatattc ccttgtaccc gtaaccaagc tcccttagca
     6061 atcatgtcaa atttacggag ctcctcatcg tctttggccc atttttgcag agcaaacgag
     6121 gaggtatagt ctgtcatttt aaagttgatg atatggcgac ctgttctagt cgttttacgc
     6181 tccacatcaa aaaccatacc ctcaaagaca atccggtttt cttcggtctc aatctcaatc
     6241 attggtgtga tggtcgcttt ttcaaaccct gcctgacgct tagctgctcg ttccttgtag
     6301 tcaaacttag gagcaggtgt ggcttcctca actggtggca tcatggcttc aagggatttt
     6361 tgggcttcca aattatcctg cacagccttt ttcacaagag cctgacgact ggaaacaaaa
     6421 tccttggtca aatgctcagt catctcctga tctgacacca tatctatggt caaggtgcca
     6481 aagccaaagg cttcaaattg cttgaccaga ttaggcagat gactgtttct aaaatgatcg
     6541 ttattcacaa aacctggcgc tgcaataatg agtttgtcat cctcataagt cactttgagc
     6601 tttgagaaag aagatttaaa actagcacta ttacacggtg catgctcaaa agcttcttgg
     6661 taataagctt gaagcagatc atctgaataa tccacctgag cagcttggat atcaaaggtt
     6721 accttaatgt cagccgcctc aaaagttctt atcaaacgat catgtaattc acgataagtt
     6781 gcaatcggta aaaccgccgc aaaggcaaaa tgaaattccc acaagcgtga caccgaatgc
     6841 acctttacct cgataatatc agcagatgaa aaggcacttg aacgtctcat gtcaagtggc
     6901 atttctatct ggtccatcaa tttagcgaat aaatctgaca taattttctc ctgattttac
     6961 taggctttat tttatcacaa ttcaagatga ttttcgagtt ttagatggaa agaaaagcag
     7021 ggctttcttc tgacgttctt tggctaattt attccaagtg atagctcagc tgaggtcatt
     7081 tttttgccaa tcttttgtcg ccaagcgctt ttggtaaagc aaaagccact ctgtatcaga
     7141 caagttcaca gcaaaaagag gccactctaa acaggcagcc tcttcatttc ttttttagtg
     7201 ttcttttgtt aaaatttcaa gggtttcgat gaggttttct gcattaactt caatgctgtc
     7261 acctgttgcc ttgattttaa tttccacgat accttcagcg gcttttttac caacagtgac
     7321 acgaattggc aaaccaatca aatcgctatc agagaatttt gaaccgacgc gttcattacg
     7381 gtcatctgtc aagacatcat accctttagc catcaagtca gcttctaact tcgccgtcaa
     7441 gtcttgggcc acttgatctt tgacattaac tgtaatcaaa tgcacgtcaa atggggctaa
     7501 ttctttaggg aagttaatac cccaagcata acggtaatcg ccttttggtg tcttgttcac
     7561 aaagagacgg gcatgctgtt caatgacagc agacaaaatg cggctaaccc cgataccata
     7621 acaacccatc acaattggaa ctgttctgcc attttcatca agaatcgttg ctcccatgct
     7681 gtctgaataa cgagtgccca acttgaagat atgaccgact tcgataccac gtgcaaactg
     7741 gagaacacca tgaccatccg gagacatttc cccttctttg acttcacgaa tatctacata
     7801 ttcagcctgg aagtcacgtc ctggattgac tcctgtcaca tggaaaccat ccttgttagc
     7861 tcctgcaacg gcattggtaa ggttttgcac tttgcggtca gccacaatgc gactaccttg
     7921 tgccaagttg acaggcccaa gtgagccaaa acctgcacca aagaaagcac gggcttcttc
     7981 ttcactagct ggctctaaaa aatcagcagc tagatagttt tttaatttaa cggtattgat
     8041 atggtcattt ccaactagca aagcaacaac aggttcatta tctgccacaa aaagcaaggt
     8101 tttgatggtt tgtgtttcat ctactgaaag aaaagcagcc acttcatcaa tcgttttgca
     8161 atgtggtgtc tcaacttctg ccaaggcatc ttcagcagct acttttgagg acggtttgta
     8221 ttcgttagtt gccatctcaa ggttggcagc gtagcttgat tctgttgaat aagcaatagt
     8281 atcttcacct gaaatcatcc aagcagccaa ttctgcctta atatcttcta agacctcttt
     8341 tggtatatca tccattgacg caatagactt gtcaagaacc acccagcgat caaggtccgt
     8401 acgagctggt gtaacggcca taaattcttg ggaatctttc ccccccatgg caccgccatc
     8461 tccaataatc cctttgaaat ctagaccagc tctggtaaaa atagcttcgt aagcttgacg
     8521 ataatcttcg taggtcacat ctaaatcttc atagttgtga tggaaactat agccgtcttt
     8581 catgataaac tcacgggtac gaagcaagcc attacgcgga cgtttttcat cacgatactt
     8641 agactgaatt tgataaaggt ttaagggcaa ttgcttgtaa gatttgaccg catcacgcac
     8701 caaagtcgta aaggtttctt cgtgggtcgg acccaagata aagtctgagt tatcacggtt
     8761 tttaagctta tagaggtcct ctccataggt ctcataacgg cctgattcac gccagagatc
     8821 agctgtcaaa agagctggtg ccaacatttc aacagcaccg atcttttcaa actcttcacg
     8881 catgatggtc ttgaatttct caatggtacg atttgccagt ggtaaataag catagatacc
     8941 agcagaaact tggc
//
LOCUS       DAUVUY010000078         8768 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.078, whole genome shotgun sequence.
ACCESSION   DAUVUY010000078 DAUVUY010000000
VERSION     DAUVUY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.078"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..774
                     /locus_tag="VOX58_001539"
     CDS             <1..774
                     /locus_tag="VOX58_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HES5878758.1"
                     /translation="ADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKP
                     VLAGKIESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQL
                     VYYIAGILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERH
                     PRTTEVIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQM
                     KALVNLLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            1149..3164
                     /gene="recG"
                     /locus_tag="VOX58_001540"
     CDS             1149..3164
                     /gene="recG"
                     /locus_tag="VOX58_001540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HES5878759.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENRSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAQNIRKKEVYD"
     gene            3457..4359
                     /locus_tag="VOX58_001541"
     CDS             3457..4359
                     /locus_tag="VOX58_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878760.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
     gene            4372..5076
                     /locus_tag="VOX58_001542"
     CDS             4372..5076
                     /locus_tag="VOX58_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878761.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(5425..6390)
                     /locus_tag="VOX58_001543"
     CDS             complement(5425..6390)
                     /locus_tag="VOX58_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HES5878762.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            6462..7850
                     /locus_tag="VOX58_001544"
     CDS             6462..7850
                     /locus_tag="VOX58_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HES5878763.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGIAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDIEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            complement(7973..8425)
                     /locus_tag="VOX58_001545"
     CDS             complement(7973..8425)
                     /locus_tag="VOX58_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HES5878764.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNIKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
BASE COUNT         2803 a         1435 c         1712 g         2818 t
ORIGIN      
        1 gcagataact ttttctactt taaaaatggt agattaacag ataagttaac aacacaagat
       61 ttgctagcct tgacagatga acaacggcaa gatatgggac tacgtcgctt agacttgtct
      121 gacctaaaac ctgtgttagc tggtaagatt gagagtcaac attatcgccc agatgatagc
      181 ctgtgtattg agcatctaac agttagagcg ggaagcaaaa tacttagatg tattgaacag
      241 cttagttttg ctgcaggaag tatttctggc attacaggat caaacgggct tggaaaatcg
      301 caattagttt attatatagc tggtatctta gacgataaaa aagcaactat taagtttcaa
      361 ggcatacctt tatctgccaa gcaacgttta tcaaagacta gtattgtctt acaagaagtt
      421 tctttgcaac tatttgcaga atcggtttca aaggaagtca acttgggcca tgaacgacat
      481 ccacggacga cagaagtaat agagcggtta tctttaacga ctcttttaga acgtcatcca
      541 gctagtttgt caggaggtga gcaacaaaga gtgatgattg ccgctagtct tttggctgat
      601 aaagatatct taatttttga tgagccaagt agcggacttg atttgttaca aatgaaggct
      661 ttagttaatc ttctcatgca gctgaagacc caacataagg ttgtcatcct catttcacat
      721 gatgaagaac ttctaagtag tgtctgcgat accatttatc aaatgctacc ttaacgagcc
      781 cctagaactg tttaaaagta ctaaaaggct tttatctgaa aataaatagg aactcttgta
      841 taatagatac aagagtcaga acgtagacaa ctccagtttt atgctcaaaa agcatgcata
      901 gctctgctga tttgacattc ggtttccgat ttttacatat aaagtgagcc aaaacagatg
      961 ctttttgctg tggtaaaaga gtggcgtcaa tacgagattg acgccactcg ggatttttga
     1021 gacctcaggc tcaaaaataa aggcatggaa ttccaaagaa cgtcgctgcc gtaagcacca
     1081 cctaaggaaa gtatcacgac aaactttgtc ttcaatctga caagagtttt tgtgaaggga
     1141 aaaagataat gatattaaca gctcctatgt ccaacttaaa gggatttgga ccaaaatcag
     1201 cagaaaaatt tcagaaatta gatatttata cagtagaaga tttactgctt tattatccgt
     1261 ttcgctatga agattttaaa tcaaaatctg tttttgattt agtggatggt gaaaaagcag
     1321 tcattacagg cttagtcgtt actccagcta atgtacaata ttatggtttt aaacgtaacc
     1381 gtttaagttt caaattgcgt caaggggaag ctgtcttaaa tgttagtttt tttaatcaac
     1441 cctatttagc tgataaaata gaacttggtc aagaggtagc tgtttttggt aaatgggatg
     1501 ccactaaatc ggctattact gggatgaagg ttttagctca agttgaagat gacatgcaac
     1561 ctgtttatcg cgtagctcag ggaatttcac agtctacttt gattaaagct attaagtcag
     1621 cttttgaaat cgatgcgcat ttggaattga aggaaaattt accagctact ttattggaaa
     1681 aataccgatt gatgggtcgt agtcaggctt gtttagctat gcatttccca aaagatatca
     1741 cagagtataa gcaagcgctc cgtcggatta aatttgaaga attattttac tttcaaatga
     1801 accttcaagt tttgaaagcc gaaaatagat ctgaaacaaa tggtttgcct attctttata
     1861 gtaaacatgc tatggagaca aagatttcct ctttaccctt tattctaaca aatgctcaaa
     1921 agcgctcttt agatgacata ttatctgata tgtcatcggg agctcatatg aatcgtttat
     1981 tgcaaggaga tgtaggatca ggaaagacag tcattgctgg tctatcaatg tatgcagctt
     2041 atacagcagg ttttcaatcg gctttgatgg ttccaacgga aatcctagct gaacaacact
     2101 acattagtct gcaagagtta tttccagatt tatcaatcgc tatattaact tcgggtatga
     2161 aagcagctgt caagcgtacg gttttagcag ctattgcaaa tggctcggtt gatatgattg
     2221 taggaactca tgctcttatc caagactcgg tacagtacca taaactgggg cttgtcatta
     2281 cagacgagca acatcgtttt ggtgttaaac agcgtagaat tttccgtgaa aagggagaaa
     2341 atcctgatgt tttaatgatg acagccaccc caattccccg aactctagca atcacagctt
     2401 ttggagaaat ggatgtttct attattgatg aattacctgc cggtcgtaaa cctattatga
     2461 cacgctgggt gaaacacgag cagctaggta ctgtgttgga atgggtaaaa ggtgaattgc
     2521 aaaaagatgc tcaagtgtat gtcatttcac cgttgattga agaatcagaa gctttagatt
     2581 taaagaatgc agtagcattg catgctgaat tatctactta ttttgaagga attgctaagg
     2641 ttgctcttgt acatggacgt atgaaaaatg atgaaaaaga tgctataatg caagatttca
     2701 aggataaaaa aagtcatatt ttagtatcca caacagttat tgaagtaggg gtaaatgtcc
     2761 caaatgcaac gatcatgatt attatggatg ccgatcgttt tggattaagt cagttacatc
     2821 aacttcgtgg gcgtgttggt cgtggatata aacaatcata cgctgtttta gtggctaatc
     2881 ccaaaactga ttcggggaaa aaacgaatga caatcatgac agaaacgaca gatggtttcg
     2941 ttttagctga gtcggattta aaaatgcgtg gttctggtga aatctttggt actcgtcagt
     3001 ctggaattcc agaatttcaa gtagctgata tcgttgagga ttatcctatt ttagaagaag
     3061 cacgcaaagt ttctgcagcg attgtttctg atcctaactg gatatatgaa aaacagtggc
     3121 aattagtggc acaaaatatt agaaaaaaag aagtttatga ttaacttaaa acaagtcgtt
     3181 gttttacaaa tatttagtta gttttgagat aaactgttat aaagtataaa gaggtcacta
     3241 tttctggcct tttttactta tgctttacga tgagactgct atgtgaagca gtattaaccc
     3301 attttcagta taactttttt attgctaaaa taatcatatt attaaaattc ttatttatat
     3361 ttttttaata aaaagaagaa cttgtcacaa ccgtttacag atgttattat gtgtaagata
     3421 ttgtattaat cgataagtac aaaaaaggag cggaaaatga cacagcaatc aatgttacta
     3481 agtattgagc aagtaaataa atcctatggt aaaaatcagg ttttaagtga tatctctttt
     3541 gatatttaca aaggagaaat ttgcggcctt gtgggtcaaa atggtgctgg aaagacaacg
     3601 ttaatgcgta ttttatctgg cctaattgga aaagattcag ggcaaatcaa acagttacaa
     3661 ccctatcgaa tgggatcaat tattgagtca ccgacacttt atcccaatat gacagctcat
     3721 gataatcttt actatgcagc gttgcaattg agattagctg atgcaaaaga gcgcatacat
     3781 gaggttttgg aacttattgg gttagaaaaa gtgagcaaaa agaaaaaggt gaaagattat
     3841 tctttaggga tgcgccaacg tttggctatt ggtctttcga tactggattt tccagaattt
     3901 ttaatcttgg atgaacccat taatggtctt gatccagcag gaatcaaaga aatgcgtcag
     3961 attattttga acctaagaga ttgctatggt attacgattt tgatttccag tcatatttta
     4021 tcagaattag acttagttgt tgatcgttac gtgattatgc ataaaggcaa agttattaag
     4081 agtatggaca aagcagagct taaagctcaa gtaaaagtac aaattgccct tcatacaagc
     4141 gatgatcagc tggtaaaaaa tagactttta gaattggggc ttagagttga gactgatggt
     4201 cagatgctac taataaaacc aactttatca gtaatggagc ttatcaaaat tgttctagat
     4261 ctgcctgttg agatttttga catttatcat catcaagtga gctttgaaca ttattacctc
     4321 gatttgttag gtaaagatgc agcatcacca ttaatttaga aagaggtttt tatgatttcc
     4381 tatcttaaag cagatttatt tcggatacgc caagagcgtt tagctctatt ttcttgtatg
     4441 ctacttgttg tcattgctgt tggtgtagcc tatagttttc gcaagacacc taaccaagtc
     4501 atcatgatac aaatcattaa tattattact gactttgtga cgcttttctt tttaacacca
     4561 gcccgtatct tttttagtga tgactataac taccgtacga ttaataatgt tgtggtcaaa
     4621 caacaaaatc gtactttaat tataatctat aaactgctag ttagttttgg cttatctctt
     4681 ttatttatta gtttaactta tcttcttagt gctttagcat ttcatttatt tactggtcaa
     4741 gcatactatg gaatagtcat agatcggatg attaaacaac tgccttttta tattgctatt
     4801 attgctttgt gtcaattttt gtttaatata cttcgtaaaa cttatcagtc aatcacagct
     4861 tatatacttt atgtccttct ttttgataaa gtatctgcta ctttagcaaa ccattttttt
     4921 aagttagata ttagcccagt tttgatgttt aaacacctat ccttgtctct tggtgataaa
     4981 cacctttcta gtactagcat attagtggcc tttggtttaa ctctttgtta tcttttaggc
     5041 aatgtctttc ttttttccaa acgagaacta aaataaaggg taatatgtgc tttgtttatt
     5101 ttaaaagaca aagaagttct tagaaaaagg tcgtcttcat cattactaaa aaaggataag
     5161 caagtagttt tgttattatt caagtaagct catacttaat gagacaaaat aaagacttgt
     5221 ttacccggta tctgattgct aagagttgga tttattggaa tactggtctc aaactgttat
     5281 agaaatgatt atgagtgcac aatctcatag tttgaaacat agttgtttat tctagacatt
     5341 taggagatgt cctaatttaa agcttaaagt actagaaatg tcccactaat atgttttagt
     5401 gggacatttc taaaggaagc tagattatcc ttcaatataa tcttttagct cttgaccagt
     5461 aagtccggcg ttgagagcaa ttaataactt tagacgggct tttggagcat tcaattcttt
     5521 tacaaacata acacctgctt cttgtagcat cgcaccacca ccttcataag catagacagg
     5581 ttctgccata ccattgaaac aacgggaaac aagaacgaca ggaataccta aagtaattaa
     5641 gtgctcgatc tccccaactg ctaatggtgg gacatttcct gcccctagag cttcgataac
     5701 tagaccttga atgctacctg gagttaatag actcaagata ctaccatcgc ccatacctgc
     5761 gtatgctttg ataatgggga tggttccaga aatgcatctc aagtcaaaac gaatacgagg
     5821 ttcagcagtt ttgaaaaaaa gtaggtcatt tttcatgatg ataccaagcg gtccgtgtgt
     5881 gggtgtttgg aaagttgaaa tattggttgt atgtgtttta gtaacatatt tagcagcatg
     5941 gatttcatcg ttcataacaa caagtacccc tttttcttta gctttatcac tacttgcaac
     6001 tctaagtgcg gtaaggtaat tatatatgcc atcactacca acttcattag aagacctcat
     6061 agcacctgtt aggacaacag gtatattagt aggcaatgcc atggtatcta aaaaataagc
     6121 tgtttcttct aaggtatctg ttccatgtgt gatgactata ccatcaaaga catcagttct
     6181 ttcttgaata tgatgataaa tagataacat gtggtgaggc gtgatgtgag gactcggaag
     6241 atttagaaaa tcactaacgg taagctgaat atcttgggct gcagcatgta ttttcgtcat
     6301 aggattatct tgatttggaa caaccctacc agagttatca gcttgcatag aaatggttcc
     6361 accagtgtgt aagactagga tttttttcat ttgattgtca aacttccttt tatttatgat
     6421 ataatctatt ctatcataaa cacaggaggc aaaagaaggt tttgacagta aaagcagtct
     6481 tttttgatat tgatgggacc cttctcaatg atcgtaaaaa tatccaaaaa acaacgcaaa
     6541 aagccatcca acaactcaaa aaacaaggca ttatggtcgg tttagcaact ggtcgtggac
     6601 cgggatttgt acaacctttt ctagaaaatt ttggattaga ttttgccgtt acttacaatg
     6661 ggcaatatat cttaacaaga gataaggtct tatatcagaa tcaattgcca aagtcgatga
     6721 tttacaaagt gattcgctat gccaacgaaa aaaagcgaga aatttctctt ggaacagctt
     6781 ctggattagc aggatcgcgt attattgata tggggactag cccatttgga caagtcatta
     6841 gtagttttgt gcccaaaagt tgggcaagaa cagttgaggg tagttttaag catttgattc
     6901 gccgaatcaa gcctcagagt tttagaaatt tagtaactat tatgcgggag cctatttatc
     6961 aggttgttct ggtagcgtca caagctgaaa ctaagaaaat acaagagaag ttccctcata
     7021 taaaaattac aagaagtagt ccttattcct tggatctgat ctctgttgat caatcaaaaa
     7081 taaaaggaat tgaacgtttg ggagagatgt ttggatttga cttgtctgaa gtgatggctt
     7141 ttggagattc agataatgac cttgaaatgt tatcaggagt tggtatcggt attgctatgg
     7201 gaaatgctga aactgtggta aaagatggag cccatttcac taccgactct aataataatg
     7261 atgggatttc caaagcctta gctcactacg gtctcatcca ttttgatata gagaagagct
     7321 ttaaaagccg tgatgaaaat tttaataaag tcaaagattt tcatcgtctg atggactcag
     7381 atactattga aacaccgcgt agttatacca tttcagaagc tggttatagg tcaggcttta
     7441 aagtagaaga aattgttgag tttctttacg cagcaagcaa gggagaccaa caacaattta
     7501 cacaggctat tttcgatttg catggtgctg ttgaccaagc agctaataag gtacaagcta
     7561 aaaaacatgt tgaaacgccc ctgattgggc aagttgatgc cttagcggat ttgctttatt
     7621 ttacttatgg ttcttttgta ctgatgggag tagatccaca acctatattt gaagcagtac
     7681 atgaagcaaa tatggctaaa atttttccag atggcaaggc acactttgac cccgttaccc
     7741 ataaaattca aaaacctgac tattggcaag agcgtcacgc acctgaagta gccataaaaa
     7801 aagagttaga caaacaatta caaaaatcac tacaacgatt cgataaatag aggtttactc
     7861 ttttaaatat caagaaataa aaagatattg actagactta gctcagcaca aaaaaacaag
     7921 caatttcgtt gtagatgctg cttgtttttt ggtgtttgaa aattgtaata gactagagtg
     7981 ttttagtgct atctcggact acaagtaagt caatcttagc gtgtcgcata atgtactcag
     8041 aagatgagcc aataaggagg cgttcaaagg tattaagacc agtagctcct accataatta
     8101 aatcagcgtt ctcacgatct ggaatatcgt gagctagtaa gtttttgggg ttgccaaatt
     8161 caataatttg tttgatgttt gtaattcctg ctatctgtgc ttgtttttca aaatcatcta
     8221 aaacatcctt agcttcttgt tctaattttt catagatgta agtatcaaaa gtagctacgc
     8281 tttgcaaggc acgggtatca ataacgtgta ctagtagtaa ggtagcgtcg ttacgtagtg
     8341 ctacgttaac gcctttattg aaagctaatt cagactcata agagccatca atagcaacga
     8401 ggatacgttt atattttagt gacatagaac tgtcctcctt taacagtttg acaagaatag
     8461 cttttattct ttttttcatt atactctttt ttgagtcatt ttgaaagcga tccattattt
     8521 ttgctaaact aaagactgta ttatcgtctc ataagacctg tgttaaaacg attagctgtc
     8581 ctaagctttg atggataaat tctttctatt ttaagtattt tggtttataa tatggaatac
     8641 gaggtgtatg agactatgaa aattattgaa aaatcatcaa aattagaaca tgttgcctat
     8701 gatattcgag ggcctgtttt ggatgaagct aatcgcatga ttgcgagtgg tgaaaaaatt
     8761 ctgcgttt
//
LOCUS       DAUVUY010000079         8588 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.079, whole genome shotgun sequence.
ACCESSION   DAUVUY010000079 DAUVUY010000000
VERSION     DAUVUY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.079"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1671
                     /locus_tag="VOX58_001546"
     CDS             <1..1671
                     /locus_tag="VOX58_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HES5878765.1"
                     /translation="ITTLGLLYYQTSSRNIRQLIERDTRQSIRQSSQFIDAYIKPLKE
                     TTSVLAKNTEIQAFASQIHQENDKQVLQLMKMVLATNSDLQAAVLVTKDGRTVSTNSQ
                     LTMKTSSDMMAEPWYKAAIDRQAMPVLTPARQLSLSSKKEWVVSVTQEVVDRAGHNLG
                     VLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVYHPKKSVYSSSKEMAAMKPYLA
                     IQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRVQRQIFWSFLGTGLLACLICGF
                     ATVLALRRWIRPIQQLQQVILAIQKGDRQLRAQETGSPELTNLAQQFNALLDQIDSLM
                     VAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWMAEFNDSKRVVEVTKSLAKYFR
                     LALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYEVQGLDVYADFVIPKLILQPLV
                     ENAIYHGIKEVDRKGMIKVTVSDTAQHLVLTVWDNGKGIEDSSLTNSQSLLARGGVGL
                     KNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLTLPKMHELMADDTQENE"
     gene            1744..2823
                     /locus_tag="VOX58_001547"
     CDS             1744..2823
                     /locus_tag="VOX58_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HES5878766.1"
                     /translation="MSLASDYFSRQTPIVEKLMAYGFEKRNNGYFYNERFMEEEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVEGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKAFPQLLQQEGIYPAYHMSKKNWISIILDDKVTDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYVCQVLETNIPNEGYRKNPNIDKLMRLRKRQQYKDGLLSFDLMKKHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            2837..3553
                     /locus_tag="VOX58_001548"
     CDS             2837..3553
                     /locus_tag="VOX58_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HES5878767.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     ITSVHVLDKDNPHVPLFTEN"
     gene            complement(3719..4192)
                     /locus_tag="VOX58_001549"
     CDS             complement(3719..4192)
                     /locus_tag="VOX58_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HES5878768.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATICGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            4334..5014
                     /locus_tag="VOX58_001550"
     CDS             4334..5014
                     /locus_tag="VOX58_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HES5878769.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYFLQYTK"
     gene            5288..6523
                     /gene="arcA"
                     /locus_tag="VOX58_001551"
     CDS             5288..6523
                     /gene="arcA"
                     /locus_tag="VOX58_001551"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HES5878770.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            6623..7051
                     /locus_tag="VOX58_001552"
     CDS             6623..7051
                     /locus_tag="VOX58_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HES5878771.1"
                     /translation="MTDKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQSYGPKCLEDG
                     EYCQSLAKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNN"
     gene            7091..8104
                     /gene="argF"
                     /locus_tag="VOX58_001553"
     CDS             7091..8104
                     /gene="argF"
                     /locus_tag="VOX58_001553"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HES5878772.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMNLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            8274..>8588
                     /locus_tag="VOX58_001554"
     CDS             8274..>8588
                     /locus_tag="VOX58_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HES5878773.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDT"
BASE COUNT         2642 a         1617 c         1821 g         2508 t
ORIGIN      
        1 attaccacgc ttggtttgct ttattaccag acgagttctc gtaatatcag gcaactaatt
       61 gaacgtgata cccggcagag cattcggcaa agttcccaat ttattgacgc ttacatcaag
      121 cctcttaaag aaacaacttc ggtgctggcg aaaaatacgg aaattcaagc ctttgctagt
      181 caaattcatc aagaaaatga caaacaggtt cttcagctca tgaagatggt tcttgcgacc
      241 aattccgatt tacaagcagc tgttctggtg actaaggatg gtcgaacggt gtctaccaat
      301 tctcagttga ccatgaaaac ctctagtgac atgatggcag aaccctggta taaagcagcc
      361 atagaccgtc aggccatgcc agtcttgacc ccagctcggc aattatccct ttcttctaaa
      421 aaagaatggg tagtttctgt gacccaagag gtagtagata gggctgggca taatttaggt
      481 gtgctaagac ttgatattgc atacccgacc attaaagcgt ctttagatca gcttcagcta
      541 ggccgccaag gctttgcctt tattgtgaat gataagcatg aatttgttta ccatcccaaa
      601 aagagtgttt acagttcttc taaggagatg gctgcgatga agccttattt agcgattcag
      661 aatggttaca ctaaggacaa gacatctttt gtttaccaaa aactcattcc taacagtcaa
      721 tggactttag tgggagtggc gtcactggat cagttgcacc gggtgcagcg ccaaattttt
      781 tggtcctttc ttggaacagg gcttctagcc tgtctgattt gtggctttgc aactgtctta
      841 gccttacgca gatggattcg tcccattcaa caattgcagc aagttattct tgctattcaa
      901 aaaggagatc gtcagttacg tgcccaagaa acgggttctc cagaattgac aaaccttgcc
      961 caacagttta atgcgctctt agatcaaatt gatagcttga tggttgccgt tgcggataag
     1021 gaaaaggcga ttgggcagta taggttacaa gccttggcta gtcagattaa cccgcatttt
     1081 ctctataaca ccttggacac tattatttgg atggcagaat ttaatgacag caagcgcgtg
     1141 gtagaagtga ccaagtctct agctaagtat tttcgtttgg cccttaatca ggggaacgaa
     1201 tacattcgtt tggcagatga actggatcac gttagccaat acctctttat tcaaaaacag
     1261 cgctatggag acaagctaag ttatgaagtg caaggcttag atgtctacgc agactttgtt
     1321 attcctaagc ttatcttaca gcccttagta gaaaatgcta tctaccatgg catcaaagaa
     1381 gtcgatcgca aaggcatgat caaggttacg gtatctgata cagctcagca tctggtgttg
     1441 actgtttggg ataatggtaa aggcattgaa gactcttcac tgaccaatag tcagagcttg
     1501 ttggctaggg gaggtgtggg ccttaaaaat gttgaccagc ggttaaaact tcactatggt
     1561 gaaggctacc acatgaccat tcatagccag tcagaccagt tcactgaaat acaattaacc
     1621 cttcctaaaa tgcatgaatt aatggcagac gacacacagg aaaatgagta aggttacagg
     1681 aagtgcatgt cagccatttc cctaagttag ggtattggag ataagaacct tagaaaggat
     1741 atcatgagtt tagcatctga ctattttagc cgacagaccc ccatagttga aaagctaatg
     1801 gcttacggtt ttgaaaaacg aaataatggt tacttctata acgaacggtt tatggaagaa
     1861 gaatttgagg cgcagcttag gattgatgag gctggcaata tctgggatcg ggttattgat
     1921 tgtgacttgg aggaggatta tttgccttta caacaagcag cctggcaagg aacctatact
     1981 gggcaggtta gagcagccta tttggaactc ttggagcgct tatcagtagc ctgttttgaa
     2041 gtcactcctt ttcagtcaat gcaagctaat agacttgcta agcatatcac caaagaatgg
     2101 tctgacccaa tggactatcc ttttgaaaag cacccagacc ttgcaaccta tcgggtagag
     2161 ggcaaatggt atgctatgat tttttctctc ttggctgata aattagacca gataccagag
     2221 agattagtag gacagacatg tgaagtgatg actgtcaaag ttaatcccaa ggctttccct
     2281 cagttactgc agcaagaagg catttaccca gcgtatcaca tgtcaaagaa aaactggatt
     2341 agtattattt tggatgataa ggtaacagat gataagcttt ggacgttagt aactcaaagt
     2401 cgtcaactag tcaacccaaa cggtctttct aatcctaatg gtcctgatta ttgggttatt
     2461 ccagctaatt tgaaatacta tgatattgat gcagaatttg cagccaatga tgtgatttta
     2521 tggacccaaa aagctggtat tgccgtggga gactatgtgc tgatttatat aacagcccca
     2581 gtcaaatcca ttcgctatgt ctgccaagtt ctagaaacca atatacctaa cgaaggctat
     2641 cgtaaaaatc ctaacattga caaactcatg cgtctccgaa aacgccaaca gtacaaggat
     2701 ggtctcttgt cttttgacct tatgaaaaaa catggcgtag ctgcagtgag agggccaaga
     2761 cgattaagcc ctcaattaat tgcattttta aaggaaaaag agtattttaa agagaacaac
     2821 taaaggagaa taagagatgg ttgaatttgt agcagatcaa agtttttggg aactttttcc
     2881 agatgccaaa ctaggtgtcg tcttattaaa ggatgttcag aatcaagcag agtcaccaga
     2941 agatgtcaaa cagctcttag cagacagcca tgagttagca aaagcttatt taacagcaga
     3001 caattttagt gataatcagg tggttcaagt ctaccgcaag gcctatcaac actttaaaac
     3061 caaaaaagga gctcgctcta gcattgaagc ccttctcaag cgggtgtcaa atggtcaaag
     3121 cattccgtcg atcaatccgc tagtggatat ttataatgca gctagtctac gttttggttt
     3181 acctgttgga gccgaagata gtgacagttt tatcggagat ttgcgtctga cgataacaga
     3241 cggtggtgac gacttttact tgattgggga tgctgacaat aacccaacct tgccaaatga
     3301 gctgtgttat aaagatgata ttggagcggt ttgtcgttgc cttaactggc gagatggcga
     3361 acgcaccatg gtgacagaac acaccaaaaa tgcatttttg attatcgaag ctcttgatca
     3421 agaaggtcaa aatagattgc aagaagctct tgacttcatt gaaggcagcg caaaaatgta
     3481 ccttcatgca atcacctctg tgcatgtgtt ggataaagac aatccacacg tgccactttt
     3541 tacggaaaac tagattagta ggatgaactg tgtaaaatag aaggttaaca aaaaagcgag
     3601 taaacgaatg tcctgtcatt taggtttttg tttactcgct ttttgatgag attttactgc
     3661 tgtcctagca aaaggtgagg atgaggtcgt atatcacata gaggttttct gttaagcctt
     3721 atttgttact aaagaagaat ggtggtgtgt aatgactaag ggtctcgtaa caagccttgg
     3781 cttgctcatt gtcttcacag acaatcaagc aagtatcatc accacagatc gttgctacaa
     3841 tttctggaat ttgcatggca tctaaaatgg agccaaagga ttgggctagt ccaggtaacg
     3901 tttttaaaat aatttgatgt tgaactatct ttaacatgac caaagcgtct tccatgtaaa
     3961 aacgaagacg atgttcccag cgggtttgtg aaatggctaa cgcttcatag tgtgtgtcgt
     4021 ttccggaagt tactttgacc aggttcaatt ccttcatgtc acgtgatagg gtagcctgag
     4081 tgatcgtgat gccatttttt tgcaaccgtt cttgtaattc ttgctgtgta tgtatagttg
     4141 tctcagagat gagggaacgt atgagttggt gacgcgtctc tttcttattc attgttataa
     4201 tcttccttta acgtaattga taacaatccg agtatagcaa aaagaggaga aggtgtaaag
     4261 tttttgatga accgcttaca aaatggcttt tttatggtaa aataaagatg atgtttagaa
     4321 aaaggagatt tgtgtgattc gaagagaaga ttatcaatac cttagaaaac taaacgattt
     4381 tcgctacttt tctattgaac aatttgacaa aattgtaggg cagatggaat tccgaaaagc
     4441 taaaaaggac catatccttt tttttgaggg agataaaaga gataagcttt ttctagtgac
     4501 ctctggttat ttcaaggtgg agcagtccga tcaatcagga accttcatgt atacggattt
     4561 catcagacac ggtaccattt ttccttacgg tggcctcttt acagatgatt attaccattt
     4621 ttcagtggta gccatgacag acgtgactta tttttacttt ccagtcgatt tatttgaaga
     4681 ttattcgctt gaaaatcgtc tacaaatgaa acacttatat agtaagatgt ctaaactgct
     4741 cgagttacat gagttgaggg ttcgaaattt gattacatca agtgctagtt ctcgtgtgat
     4801 tcagtctctg gcgattttgc tggttgaaat gggtaaagac agtgatacct taccttttca
     4861 attaacaaca acagatattg ctcaaataag tgggacgaca agagaaactg ttagccatgt
     4921 gcttagggat ttgaagaagc aagaattgat taccataaaa ggtaagtatt tgacttatct
     4981 agataaagac tattttttac aatacacaaa gtgaattaaa ggtcagaaca ctttatttta
     5041 aaagtttttc tgacttattt taatttataa gtataaattt ttacactaaa atgtaaaaaa
     5101 catacaagta aacgaataca aatctataaa aataaaaata acgccccgaa aatgtaagcg
     5161 attactcaaa atagctgtga gaaataccct atttttatct tttttaagga gtacactagg
     5221 aatataagca agagttaaga atcttgacgg tgaattcaca aaaaccaatt agaaagaggt
     5281 aattcttatg actgctcaaa caccaattca tgtttattct gaaattggaa aattaaaaaa
     5341 agtgttgctc cacagacccg gtaaagaaat tgaaaatctt atgccagact acttggaacg
     5401 cttgttgttt gatgatattc cattcttaga ggatgcccaa aaagaacatg atgcttttgc
     5461 ccaagctttg cgtgacgaag gtattgaagt cttgtacctt gagaccttag ctgcagaatc
     5521 tcttgtcact ccagaaattc gcgaagcttt catcgatgaa tacctaagtg aagctaatat
     5581 ccgtggaaga gctactaaaa aagccattcg tgaattgttg atggctatcg aagacaatca
     5641 agaattgatt gaaaaaacca tggcaggagt tcaaaaatct gaacttccag agatcccagc
     5701 atctgaaaaa ggcttgactg acttggttga atccaattac ccatttgcga tcgacccaat
     5761 gccaaacctt tatttcacac gggacccatt cgcaactatc ggtacaggtg tttctcttaa
     5821 ccacatgttc tcagaaacac gtaaccgtga aaccctttat ggtaaataca ttttcactca
     5881 tcacccaatt tatgggggtg gcaaagtgcc tatggtttat gaccgtaatg aaaccactcg
     5941 cattgaaggt ggggacgaac ttgttctttc aaaagatgtg cttgcggttg gtatttctca
     6001 acgtacagat gctgcttcta ttgaaaaatt gttggttaac atctttaaac aaaaccttgg
     6061 cttcaagaaa gtattggcct ttgaatttgc aaataaccgt aaattcatgc acttagacac
     6121 tgtctttacc atggttgact atgacaaatt taccattcac ccagaaattg aaggcgacct
     6181 tcgtgtttac tctgtcactt acgacaatga agaacttcat atcgttgaag aaaaaggtga
     6241 tttagcagaa cttcttgctg ctaaccttgg tgttgaaaaa gttgacctta tccgttgtgg
     6301 tggtgacaac ttagtagcag caggtcgtga acaatggaac gatggttcta acacccttac
     6361 tatcgcacca ggtgtggttg tggtttataa ccgtaacacc attaccaatg ctattcttga
     6421 atctaaaggc ttgaaattga tcaagattca cggaagtgaa ttggttcgcg gtcgtggtgg
     6481 acctcgttgt atgtcaatgc catttgaacg tgaagatatt taataagcta tggtaaaggt
     6541 ggttataggt cagaagcctt tttaaagggc agctagtgtt tatcttcgct tctgtgtctt
     6601 tgtccttcaa tgaaattttg tcatgacaga taaatttgat gccaatgacg aaacaagaac
     6661 ggtttatgca gtcgtttatg acaatgacca gcccgtttca acaggacaat ttttagctga
     6721 aacgaaaata gaagcacgat tgacacgcat tgtaacctta gcagattatt gtggttgcgg
     6781 ttatggtgcc aaagtcactg aagcgctaga aacttatacc agacgagaag gcttttacca
     6841 actaaccatt cacagtgaac tgacagcaca aaccttttat gaaaacctag gttatcagtc
     6901 ctatggtccc aagtgtttag aagatggtga gtattgtcag tcccttgcaa aaaccattct
     6961 taaatgggag aagaatatgg acatagcaat gctaattgcg attgttggtg gtctattagg
     7021 ctgctatctc tatctcacaa aaaataatta atcaaaacag attacagtta acactcgaag
     7081 gagacaatag atgacacaag tatttcaagg acgtagcttc ctagcagaaa aagattttac
     7141 acgcgctgaa ttagaatacc ttattgattt ttcagcacat ttgaaagatt tgaaaaaacg
     7201 tggtgtgcct catcactact tagaaggtaa aaacattgcc ctcttgtttg aaaaaacatc
     7261 aactcgtacg cgtgcagctt ttacaacagc agccattgac ctaggtgctc acccagaata
     7321 cctcggtgcc aatgacatcc aacttggtaa aaaagaatca acagaagaca ctgctaaagt
     7381 attgggtcgt atgtttgatg ggattgaatt ccgtggcttt agccaacgca tggttgaaga
     7441 attggcagaa ttctcaggtg tgccagtttg gaatggtttg actgatgaat ggcacccaac
     7501 ccaaatgctt gctgactatt taacagtgaa agaaaacttt ggcaaacttg aaggccttac
     7561 ccttgtttac tgtggtgacg ggcgtaataa cgttgccaat tcccttcttg tgactggtgc
     7621 tatccttggt gttaatgttc atatcttctc accaaaagaa cttttcccag aagaagagat
     7681 tgtgacattg gctgaaggct atgctaaaga aagtggcgct cgtatcctta tcacagaaga
     7741 tgctgacgaa gctgttaaag gcgcagacgt gctttacaca gatgtttggg tatcaatggg
     7801 tgaagaagac aaattcaaag aacgtgttga attgcttcaa ccatatcaag tcaacatgaa
     7861 ccttgttcaa aaagcaggaa atgacaagct tatcttcttg cactgcttgc cagctttcca
     7921 tgacacaaat accgtttatg gtaaagacgt tgctgaaaaa ttcggcgtga aagaaatgga
     7981 agtcactgat gaagtcttcc gcagcaaata cgctcgtcat tttgaccaag ctgaaaaccg
     8041 catgcacact atcaaagcgg ttatggcagc aacccttggt aacctcttta ttccaaaagt
     8101 gtaataagga ctggtactcc ttaactcatt tcacatatca atgtgacccc tttaatttaa
     8161 caatttaact acttaaaaac gtactttaac tacatgacaa gtcgttctgg ttttcgcgtc
     8221 cttgaccgga actagaacgg cttattttta tgaaaaggta agaggtaatc attatgacag
     8281 aagaaaaaaa acggggtttt agaattcctt cttcttacac cgttcttttt atcattatag
     8341 ccatcatggc agtgttaact tggtttattc cagctggcgc ctacgaaacc gctaagggcg
     8401 gtggtgttat ttcaggaacc tacaaaacag tagcatccaa tcctcaagga ttttttgaca
     8461 tcttgatggc tcccgttcga ggcatgttag gtgttgaagg cactgatggt gctattcaag
     8521 tgtctttctt tatcttaatg gttgggggct ttttaggagt tgtcaacaaa acaggtgctc
     8581 ttgacaca
//
LOCUS       DAUVUY010000080         8573 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.080, whole genome shotgun sequence.
ACCESSION   DAUVUY010000080 DAUVUY010000000
VERSION     DAUVUY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.080"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..539
                     /locus_tag="VOX58_001555"
     CDS             <1..539
                     /locus_tag="VOX58_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HES5878774.1"
                     /translation="PFKDLTEVNDIESLNYAKEAMENGVSAARVMDSIRKVGRDNART
                     PMQWSKDTHAGFSEAKETWLPVNPNYQDINVADALADPDSIFYTYQKLIALRKEQDWL
                     VEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFAKDLTGAQVIITNTDVDTV
                     FETKHLQPWDAFCLKLKA"
     gene            737..4234
                     /locus_tag="VOX58_001556"
     CDS             737..4234
                     /locus_tag="VOX58_001556"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HES5878775.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGLQSSSKEAKPVLDSSLANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQIFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADL
                     TKSDKGVWRAHLTSDSIKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSVNPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSIAYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYRTKVS
                     DDKVPNKATLIDAVAKYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            4349..4723
                     /locus_tag="VOX58_001557"
     CDS             4349..4723
                     /locus_tag="VOX58_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HES5878776.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            4839..5204
                     /gene="yajC"
                     /locus_tag="VOX58_001558"
     CDS             4839..5204
                     /gene="yajC"
                     /locus_tag="VOX58_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HES5878777.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            5423..6172
                     /locus_tag="VOX58_001559"
     CDS             5423..6172
                     /locus_tag="VOX58_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HES5878778.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            6185..6979
                     /locus_tag="VOX58_001560"
     CDS             6185..6979
                     /locus_tag="VOX58_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HES5878779.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            7052..8311
                     /gene="rseP"
                     /locus_tag="VOX58_001561"
     CDS             7052..8311
                     /gene="rseP"
                     /locus_tag="VOX58_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HES5878780.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLDETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMSDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
BASE COUNT         2562 a         1623 c         1938 g         2450 t
ORIGIN      
        1 acccttttaa agacttaacc gaggttaatg atattgagtc ccttaactat gctaaggaag
       61 ctatggaaaa tggagtgtca gctgcgcgtg tgatggatag catccgcaag gtaggacgag
      121 acaatgctag aacgccgatg cagtggtcta aggacacaca tgctggcttt tctgaagcaa
      181 aagagacttg gttgccggtc aaccccaact accaagacat caatgtggcc gatgcccttg
      241 ctgatccaga ctctattttt tacacctacc aaaaattgat tgccttgcgt aaggagcagg
      301 actggctagt tgaagcggat tatcacctct tgccaacagc tgataaagtg tttgcttatc
      361 agcgtcagct tggagaagag acgtatgtga ttgttgtcaa tgtgtctgat gaggagcaag
      421 tctttgccaa agacttaaca ggtgcacaag tgatcatcac caatacagat gtggacacgg
      481 tgttcgagac caagcactta caaccttggg atgccttttg tctcaaactg aaggcctagc
      541 agttgtatgt gatttgagtt tacccaacag ccaagcgagt ggaaactcgt tttggctgtt
      601 tgatttttga gatcctatta aggggtgata gtcattgttt gagctgtgtt ctaagttgaa
      661 gtgcggcatg tcttgcaagc gcttgcgcaa tgtgataaga tagtcttgtt tgaatgttat
      721 actaaatgga ggtcttatga aaaagaaagt caaccaagga tcaaagcgct atcaatatct
      781 gttaaaaaag tgggggatag gttttgtaat cgctgcaact gggactgtcg tgttagggtg
      841 cacccctagt atcttaacac atcaagttgc tgctaaaacc attgttggac tagcccgcga
      901 tgaagctcaa caaggagatg gcaatgctaa atctggtgat ggtcttcaat cgtctagcaa
      961 ggaggcaaaa ccagttttag acagctcgtt agctaatcct gctagtattg ctgagcatca
     1021 tttgcgtatg cattttaaaa cattgccagc tggtgagtcg ctaggaagct tgggactttg
     1081 ggtgtgggga gatgtggatc aaccttcaaa ggattggcca aatggtgcta tcaccatgac
     1141 aaaagcgaaa aaagatgact atggctatta tctagatgtg ccactagcag ctaaacaccg
     1201 ccagcaagtg tcttatctca ttaataataa agctggagag aatctttcaa aggaccagca
     1261 catctcgctt ctcacaccaa aaatgaatga agtttggata gacgagaatt accatgcgca
     1321 cgcttatcga cctttgaaaa aaggttacct tcgaatcaac taccacaatc aatcgggaca
     1381 ctacgataac ttagctgtct ggacctttaa agatgtcaaa accccaacga ccgactggcc
     1441 aaatggactt gacttgtcac ataaagggcc ttatggagct tatgttgatg tccccttaaa
     1501 agaaggagct aacgaaatcg gatttttaat ccttgataaa agtaagacag gagatgctat
     1561 taaagtgcaa ccaaaagatt atctgtttaa agagttagac aatcatactc agatttttgt
     1621 caaagacact gacccaaaag tctacaacaa tccttattat attgatcagg ttagtctcaa
     1681 aggagctgaa caaaccacgc caaatgagat taaagccatt tttacgacct tagatgggct
     1741 tgatgaagat gcggtgaaac aaaacatcaa gatcactgac aaagcaggga aaactgttgc
     1801 aattgatgag ttgacacttg acagggataa gtctgtaatg acattaaagg gtgattttaa
     1861 ggcgcaaggt gcagtctaca cggttacatt tggagaagtt agccaagtcg ctcgccaatc
     1921 ctggcaatta aaagataaac tctatgctta cgatggtgaa cttggagcta ccctagctaa
     1981 ggatggttct gttgatttag cgctatggtc tccaagtgct gatactgtta aggttgtcgt
     2041 ttatgataaa caagatcaga caagggtggt tggtcaagct gatttgacca agtcggacaa
     2101 gggtgtttgg agagctcatc tgacttctga tagtatcaag ggcattagtg attacacagg
     2161 ctactattac ctttatgaaa tcacgcgcgg tcaggaaaaa gtcatggttt tggatcctta
     2221 cgccaaatct ctcgctgcct ggaatgatgc gactgctact gatgacatca aaacagcaaa
     2281 agctgccttt attgatccaa gcaaactagg accaacaggc cttgattttg ccaaaattaa
     2341 caactttaaa aagcgtgaag acgctattat ctatgaagca catgtgcgag attttacgtc
     2401 agataaggct ctagaaggca agttaacaca cccttttggg actttttcag ctttcgttga
     2461 acagctagac tatctcaaag acttgggggt tacccacgtt caattgctac cggttttgag
     2521 ttatttttat gccaatgagc tggacaagag ccgctcaaca gcctacacgt cttcagacaa
     2581 taattacaac tggggttatg acccacaaca ctactttgcc ctttctggca tgtattcggt
     2641 aaatcctaac gaccctgctt tacgtatcgc agagcttaaa aaccttgtca atgagattca
     2701 caaacgtggt atgggtgtta tttttgatgt ggtttataac cacacggcta gaacctatct
     2761 ctttgaagat ttggaaccca actactatca ttttatgaat gctgatggca cagctagaga
     2821 gagttttggc ggaggtcgtc taggaacgac acatgccatg agtcgtcgta tcttggtgga
     2881 ttcgattgct tatctgactc gtgaattcaa ggtagatggt tttcgtttcg acatgatggg
     2941 tgaccatgat gcggcagcta ttgagcaagc ctttaaggca gccaaagcca ttaatccaaa
     3001 taccattatg attggcgaag gctggcgtac ctaccaaggt gatgagggga aaaaagaaat
     3061 tgcggcagat caagattgga tgaaagcaac caatacggtc ggtgttttct ctgatgatat
     3121 cagaaatacc ctcaagtcag gtttcccaaa tgaaggcaca gcagccttta ttactggtgg
     3181 cgcaaaaaat ctagaaggac tgttcaaaac gatcaaagca cagcctagta actttgaagc
     3241 agatgcccca ggagatgtag tgcagtatat tgcagcccat gacaacctga ccttacatga
     3301 tgtcattgcc aaatccatca ataaggatcc taaagtggct gaagaagaga ttcataagcg
     3361 tattcgtcta ggaaatacca tgattttaac tgctcaaggg actgccttta tccattctgg
     3421 tcaggaatat ggacgaacca agcagcttct aaatcccgac tacaggacaa aggtgtctga
     3481 tgacaaggtg ccaaataagg cgactctgat tgatgctgta gcgaaatacc cttacttcat
     3541 ccacgattct tatgattcgt ctgatgcggt caatcatttt gactgggcaa aggcaacaga
     3601 ttccatagct cacccgatta gcaaccaaac aaaagcctat acacagggcc taattgcgtt
     3661 gcgtcgctca acagatgcct ttacaaaagc aaccaaagct gaggtagatc gggatgtgac
     3721 cttgatcacc caagcaggac aagatggtat tcaacaagag gaccttatca tgggttacca
     3781 aacggtggca tcaaatggag atcgctatgc tgtctttgtc aatgcagaca acaagacccg
     3841 caaggtagtt ttacctcaag cctaccgcta tttgctagga gcccaagtgc ttgctgatgc
     3901 tgagcaggct ggtgttactg ccattgctaa gcctaaggga gtccagttta ccaaagaagg
     3961 cttgactatt gatggcctaa ctgccctggt cctcaaagta tcctcaaaaa cagctaatcc
     4021 ctctcagcaa aagagtcaga cagacaatca tcaaaccaaa acaccagatg gctcaaaaga
     4081 cctagataaa tcattaatga ctaggccaaa aagagctaaa acaaaccaaa agctcccaaa
     4141 aacgggtgaa gcctcctcaa aaggcttatt agcagctgga gtagctctgc ttttattggc
     4201 tattagcctg ttgatgaagc gccaaaaaga ttagggtggt gttaatagag acaaaaagat
     4261 tgctgtttgc ggattctttt tgacggctat aaaagctctg agctgtgaaa agaccttgtg
     4321 attgagaaaa ttgctacttt agtgtatcat gagaccaaaa gagaaggaga catccatgac
     4381 atttgaagaa atcgttgcga attttatacc gtcgagtgtt gcagaagtca catcagctat
     4441 tgctagtggc aaagacatga ttgtcttttt aggacgttcg agctgtcctt attgtcgccg
     4501 ctttgctcct aagttggcac aagtggcaac agataatcaa aaagaagtct attttgtgga
     4561 tagtgaaaac gctgctgatg ctgctgagct tgctgccttt cgtgagaact accaattagt
     4621 gacagttccg gctttgctgg ttagttacga tcagcaccaa agggctgtat gtgattcaag
     4681 tttgacacca gatgacatct tagctttttt gactcgtgag taaaggactg cgcttagcgt
     4741 ctcgtttaaa gaagaaatca ggtaacgtct agtcttgcta tacctcttga tttttgatat
     4801 agtaagacat gacttttata gaataaaaag gagacattat gggatttcca acaattttaa
     4861 tgtttgtagt gatgcttgga ttgatttggt ttatgcaacg tcaacaaaaa aaacaagcgc
     4921 aagaacgcca aaaccagtta aacgctattg aaaaaggaga cgaagtcgta acgattggtg
     4981 gcatgtttgc gattgttgac gaagtcgata caacagctaa aaagattgtg ttagatgtgg
     5041 atggcgtctt tttgacattt gagctatcag ctattaagcg tattgtaaca aaagctacca
     5101 cagaaacgac actggttgaa acagaagaaa ccagtgttgt taatgagcct gctgttgaga
     5161 cagtagatag cactgacact gacagtgcca ttgaaagcca ttaaggcggg gaaagtttgg
     5221 atgaaatatg ccaaactttt ttgttttgat ccctggactg aaacagttat tctgatgcta
     5281 acaggttcat gaaaagctga cacatctatc agaggatagg tgaacatttt gtgaagataa
     5341 gatctaattg ataaaaaaca agccaaaagg cgtgaaatat gataaaatat gagataagta
     5401 tgcttatcaa taaggaaaaa acatgtttgg attaaaagcg aaatcaacaa aaaaagtttt
     5461 ggggagtatc ccaaagcata ttggaattat catggatggt aatggacgtt gggccaaaaa
     5521 aagactaaaa cctagagtgt ttgggcataa agctgggatg gatgctttac aagaagtaac
     5581 gattactgca tctgagttgg gggttaaagt tctgacagtc tatgcctttt cgacagaaaa
     5641 ctggtccaga ccccaagatg aggtgtcttt tatcatgaat ttaccagtaa ccttctttga
     5701 caaatatgtg cctgtgcttc atgagaacaa tgtaaagatt caaatgattg gagagactag
     5761 tcgtttacct gaagatacct tggcagcgct aaatgcagcc atcgacaaaa caaaacgtaa
     5821 tacgggttta attttgaatt ttgctctcaa ttatggagga cgtgctgaga ttaccagtgc
     5881 tgttcgcttt attgctcaag atgtcttaga tgccaaatta aatccaggag atattacgga
     5941 ggatttgatt gctaattact tgatgacaga ccatctgcca tatctctacc gagaccccga
     6001 tttgattatt cgcaccagtg gtgagctgcg tttgagcaat ttcttgcctt ggcaatcagc
     6061 ttacagtgaa ttttatttca caccagtctt gtggcctgat tttaaaaagg cagagctact
     6121 aaaggccatt gcggattata accgccgcca acgtcgtttt ggcaaggtct aaaaggagat
     6181 agttatgaaa gaacgtgttg tttggggggg agtagcagta gctattttcc taccttttct
     6241 cataatagga aacctaccat tccaattatt tgtaggcgtt ttggccatga ttggggtatc
     6301 agaattactg aaaatgaaaa gattagaagt cttttctttt gaaggtgttt ttgccatgtt
     6361 agccgccttt gtattggcag ttccgatgga ccattacctc acttttttac caattgatgc
     6421 gaatgttgct ttttacagct taatggtttt tttcattttg gcaggtactg ttttaaatag
     6481 cagagcttat tcatttgatg atgcggcctt tccgattgca actagttttt atgttgggat
     6541 tggttttcaa catttgatca atgctcgcct gtcaggaatt gacaaggtct ttttggcctt
     6601 atttatcgtt tgggccactg atattggagc atatctgatt gggcgtcagt ttggtaggcg
     6661 caaactattg cctacagttt ctccaaacaa aaccattgaa ggtagtttag gagggatcgc
     6721 ttgtgcagtc cttgtgtctt ttatctttat ggtgattgat aggtctgttt atgcgccaca
     6781 tcactttttg accatgttgg ttttggtcgc tcttttttcc atttttgccc aatttggtga
     6841 tttggttgaa agtgctctca aacgtcactt tggtgtcaaa gattcaggta agcttatccc
     6901 gggacatggt ggtattttag accgctttga ttccatgatt tttgtttttc ccatcatgca
     6961 tctctttggt ttattttaat cttaagaggt aatgtttgga gtcgcaacaa cgcgacaaaa
     7021 ctcaaaaaag caatagactg aaaggaaatc tatgttagga ataataacct ttattattat
     7081 ttttggtatt ttagtgattg tccatgaatt tggacatttc tattttgcta aaaaatcagg
     7141 cattctagta agagaatttg ccattggaat gggccctaaa attttttctc atgttgacca
     7201 aggaggaact ctttatacct tgaggatgtt acccttgggt ggttatgtgc gaatggctgg
     7261 ttggggtgat gacaaaactg agatcaaaac aggtactcca gcaagtttaa cccttaatga
     7321 gcaaggtttt gttaagcgca tcaacttgtc tcaaagtaag ttagacccaa cgagtctccc
     7381 aatgcatgtt acaggctatg acttagaaga tcagctgagt attactggct tggttttaga
     7441 tgaaaccaag acatataagg tcgctcacga tgctactatt gttgaagaag acggtactga
     7501 gataagaatt gctccgcttg atgttcaata tcaaaatgct agtattggcg gacgtttaat
     7561 caccaatttt gcaggtccca tgaataattt tattttaggg attgtggttt ttatcctctt
     7621 ggtcttttta caaggtggga tgtcagattt tagtagcaat catgtccgtg ttcaagaaaa
     7681 tggagcagca gctaaggctg gtcttcgaga taatgaccaa attgtcgcaa ttaatggtta
     7741 taaggtgact agctggaatg atctcactga ggctgtagac cttgcaacac gggatctagg
     7801 cccgtcacag accattaagg tcacctacaa gtcacatcag cgcttaaaaa cggtggctgt
     7861 gaaaccacaa aagcatgcaa agacatacac gataggagtt aaggcgagtc tgaaaacagg
     7921 atttaaggat aagctcttag gcggtctaga attagcttgg agtggggcgt ttactatttt
     7981 gaatgctttg aaaggattga tcactggctt tagtctcaat aaattaggtg gacctgttgc
     8041 catgtatgac atgtccaatc aggctgctca aaacggctta gagtcagtct tatctcttat
     8101 ggcaatgctt tcgatcaatt tagggatctt taacctgatt ccgattcctg cacttgatgg
     8161 gggaaaaatc ttgatgaata tcattgaagc cattcgtcgc aagcctatca agcaagaaac
     8221 agaggcctat atcaccctag ctggggttgc tatcatggtc gtattgatga ttgctgtgac
     8281 atggaatgat atcatgcgcg tctttttcta atcaagatag gtcatcttaa gctgacctta
     8341 tcatgttaaa gatcgaaaaa gacctcaacc ctaagatttg caagggttga ggtcttactc
     8401 aaaatgaaag gaatacacat tcaaattatt gaataatccc gcttcactct tacttgatgt
     8461 ttccagaagg gtttgaggat aaggggatgt attaaaacgg aataatctat gaaacaaagt
     8521 aaactgctta tcccaacctt gcgcgaaatg ccaagtgatg cccaggttat cag
//
LOCUS       DAUVUY010000081         8462 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.081, whole genome shotgun sequence.
ACCESSION   DAUVUY010000081 DAUVUY010000000
VERSION     DAUVUY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.081"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            143..892
                     /gene="truA"
                     /locus_tag="VOX58_001562"
     CDS             143..892
                     /gene="truA"
                     /locus_tag="VOX58_001562"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HES5878781.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            882..1646
                     /locus_tag="VOX58_001563"
     CDS             882..1646
                     /locus_tag="VOX58_001563"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HES5878782.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHLGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            1636..2106
                     /locus_tag="VOX58_001564"
     CDS             1636..2106
                     /locus_tag="VOX58_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HES5878783.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            2120..2680
                     /locus_tag="VOX58_001565"
     CDS             2120..2680
                     /locus_tag="VOX58_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HES5878784.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQFMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(2745..3590)
                     /locus_tag="VOX58_001566"
     CDS             complement(2745..3590)
                     /locus_tag="VOX58_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HES5878785.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            3911..5194
                     /locus_tag="VOX58_001567"
     CDS             3911..5194
                     /locus_tag="VOX58_001567"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HES5878786.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            5406..5981
                     /locus_tag="VOX58_001568"
     CDS             5406..5981
                     /locus_tag="VOX58_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HES5878787.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            6238..7842
                     /locus_tag="VOX58_001569"
     CDS             6238..7842
                     /locus_tag="VOX58_001569"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HES5878788.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRNVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            8010..>8462
                     /locus_tag="VOX58_001570"
     CDS             8010..>8462
                     /locus_tag="VOX58_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HES5878789.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQL"
BASE COUNT         2770 a         1271 c         1823 g         2598 t
ORIGIN      
        1 agagagtttg gaggaaatag ctttcttata gcttttaaat cgctagtggc ttagagtaag
       61 cttcaatacg tctttcattt gtttgggtac agtttgatta aaaatagttt ctcttacgtt
      121 taagaaatgt taaaatagta acatggtaag atacaaagca acgatttcct atgatgggac
      181 tcttttttcg ggttttcaaa gacaaagaca tttacgtacg gttcaagaag agattgaaaa
      241 aacactctat aaattaaata acggtacaaa aattataatt catggtgcag gacgtacgga
      301 tgcaggagta catgcttatg gacaggtgat ccattttgat ttgcctcaag aacaagaagt
      361 tgaaaaatta cgatttgctc ttgatacaca gactcctgaa gatattgatg ttgttaatat
      421 agaaaaagtg gctgatgatt ttcattgtcg ttatcagaag catttaaaaa cttatgaatt
      481 tttagttgat aatggtcgac ctaaaaatcc gatgatgcga cactatacca ctcattaccc
      541 ttatactctt aatattaaac ttatgcaaga agctattaac ggtctggtag gtacacacga
      601 ttttactggt tttacagcag caggaacttc ggtacaaaat aaggtgcgaa cgattaccaa
      661 agcaacggta tctcgggatg aaaaaactga ttttttggtg tttacttttt caggaaatgg
      721 ttttctctat aagcaggtga ggaatatggt gggaactttg ctgaaaattg gtaatggtca
      781 aatgccagtt gaacaagtta aagtgatttt gagctccaaa aatcgtcagc tagctggacc
      841 aacgatatct ggaaatgggc tttacttgaa ggagatttgt tatgaaaact gattatatcg
      901 tgactatatc tgggaacgat attttaagtg gaggtggtct ctatgctgat ttagcgactt
      961 acataaggta tgacctacag gcctttgtag cggtaacttg tttaactact agatctgaag
     1021 agggctttag tctatttccg gtagctaaag aaattttccg tgatcagttg aatagcttta
     1081 cgaacgctcc aatttctgct attaaaattg gtttgttacc taatgcggaa atgtgtgaaa
     1141 tagttttgga ttttataaag gggcatctgg gaatcccagt ggttttagat cctgttctag
     1201 catgtaaaga aatagatgat gttaaaatag ttcctctaag acaggagatt ttacaattat
     1261 tgccttatgt aactgttgtt actccaaatt tggtagaggc tcagctatta tctcaaaaag
     1321 agattgtttc tttaaaagat atgcaagaag ctgctaaata tttttatcaa cttggggcaa
     1381 aacaggtggt tatcaagggt ggtaatcgat ttagtcagaa aaaggctatt gatctttttt
     1441 atgacggtaa agagattgtt accttggaat gtcctgtact tgagaaaaat aatattggcg
     1501 caggctgtac ttttgcatct agtatcgcta gtcagttagt aaagaaaaaa acgccactag
     1561 aagctgtgaa aaactcaaaa gagttagtct accaagctat tttacaatct gatagatacg
     1621 gagtaaaaca aagttatgca aaatagtaag atacgacaaa tgagtttaac agggatattg
     1681 acagcattgg ttgttgtttt aggaagattt gtcatgctac ctactccaac aggtttttta
     1741 acgctgttgg atgcaggaat ttatgctgtc agcttttcct ttggatcagc tcaaggagct
     1801 attgtgggag ggttatcagg ttttttaatc gatttagtgg caggataccc tcagtggatg
     1861 tttcatagtt tgattgctca cagtgtccaa gggtattttg cgggatggag agggcgcaag
     1921 cgttggcttg gtgttgtgat aggttctttt atcatgattt tttggtactt tttaggctct
     1981 cttatgcttg gttatggcct ttcgggatct ttagccggta tttggggaaa cgtcatgcaa
     2041 aatacattag ggctttttgt tggttttatt atctttaagg ccatattaag gcaaaaaaag
     2101 agataaggag agtgagaagt tgttgaataa tttagagaaa caaacaaggg aaatcgtcat
     2161 tgatgttgta gaacgctctg ctattcaacc aggtaatctt tttgttctag gtctttcatc
     2221 tagcgaaatt ttgggtagta gaattggaaa acaatctagt ctagaagtag gtcaaatagt
     2281 tgttgaagtt gtgttagatg aattaaataa gcgtggtgtt catttggctg tacaaggttg
     2341 tgaacacgtc aatcgtgcct tggtagtaga gaggcatgtg gcagaatcta agcagttaga
     2401 gattgtcaat gtggttccga atcttcatgc tggtggcagt gctcagatgg ctgcttttca
     2461 atttatgtca gaccctgtcg aagtagaaga agttattgca cacgctggac ttgatatagg
     2521 tgatactgct ataggaatgc atattaagcg tgttcagatc cctctgattc cttgtcaacg
     2581 agagctagga ggtgctcatg tgacagcttt ggctagtcga ccaaaattaa ttggtggagc
     2641 tagggctgat tacaatatgg atattatccg taaatcctaa accctttttt ctggaataaa
     2701 acaattggtg ttttttctag aaaaaaggat tttttaagat tatattaaga ttttgaggtg
     2761 atagaatagc tgattgcagt cggtaattga atgccttctt tgagtaaagc ttcttgataa
     2821 agtctataaa aagtatggta aatcttaaat tgttctccat tttctgtgaa aatagcaatt
     2881 ctaaaagtga actgtccatt agagttattt tgaggcccta aaatatttgg tttgccaacg
     2941 atttgaggat ggtttggcaa ttccttttga ttcacttcat caatgattcg agtgacttgg
     3001 ctcaaattaa cagtggaata aagaggaatt tcaattaagg cacgcatatt accacgagat
     3061 ttgttgctga caacggtgat gctacggttt gggatgaaat gtagtgttcc atcgaatcca
     3121 cgaatttgag tagttctaat tccaacacca aagacactgc cttctatgtc agagatagta
     3181 acgttatctc ctacttcaaa ttgattctca aataagataa aaaagccatt gacaacgtca
     3241 gataaaaatc cctgtgcacc taacccgatt gctactccag caattccagc tcctgctaga
     3301 agactagaga caggtatgcc aaataagcta agtatccaat aaatgagaag aaaatacaat
     3361 aagtaattta aaatattgtg agtgagctta gagagtgttt ttttgcgagc ttcactttgt
     3421 cttgaataag caaacgattt gttaatggtt ttttcaaaaa gatagttagt aacacgtttt
     3481 aaaatagcaa aaaagagtaa tagaataatg agagacacta gctttttaaa aatcgttaag
     3541 ccaatatttt cgatatgaga ctgctctagg taatgtaata taaatgtcat gagaaatcct
     3601 ttctttgata gtttatcaaa aaaaaaccaa tcttgaaaag agataaatta atattttaac
     3661 caaagttatg ataattgttt tatcataaag aatgccatag tcatccgttg tgataatgct
     3721 tgaaaatagt cagttagtaa gcgaaaaagt ctaacattaa cctttataaa ttgaaaataa
     3781 ctgttagtag actaatgaca ggtagatact cttaatagcg cgtttgattt cacgcataaa
     3841 aagctttaaa aacgctttaa attatgataa aatgaactgt atcgaaatta ttttaaggag
     3901 aaatgactta atgtctacat catttgaaaa caaagctaca aatcgtggcg tgattacatt
     3961 tacaattagt caagataaaa taaaaccagc tcttgataaa gcttttaata aaatcaaaaa
     4021 agacttaaat gcaccaggtt tccgtaaagg acacatgcct cgtccagtct tcaaccaaaa
     4081 atttggtgaa gaagttcttt atgaagatgc tttgaatatt gtattgccag aagcttatga
     4141 agcagctgtg acggaacttg gtcttgatgt ggttgcacaa ccaaaaatcg atgttgtgtc
     4201 aatggaaaaa gggaaagagt ggacactttc tgctgaagtt gtgacaaaac ctgaagtgaa
     4261 acttggtgat tacaaaaacc ttgttgtaga agttgatgct tcaaaagaag tttcagatga
     4321 agacgtggat gctaaaattg agcgcgaacg tcaaaacctt gcggaactca ttattaaaga
     4381 cggtgaagca gctcaaggtg acactgttgt gattgacttt gttggttcag ttgatggtgt
     4441 tgagtttgat ggcggtaaag gagataactt ctctcttgaa cttggttcag gacaatttat
     4501 cccaggtttt gaagatcaac tagttggtgc taaagctggc gatgaagtag aagttaatgt
     4561 cacattccca gaatcttacc aagcagaaga tcttgcaggt aaagccgcta aatttatgac
     4621 aactattcac gaagtcaaaa caaaagaagt accagagctt gatgatgagc ttgcaaaaga
     4681 tattgatgaa gatgttgaca cacttgaaga cttaaaagta aaatatcgta aagaacttga
     4741 agcagctcaa gaaactgctt atgatgatgc tgttgaagga gctgcgattg aattagcagt
     4801 tgcaaatgct gaaattgttg atttacccga agaaatgatt catgaagaag tcaaccgttc
     4861 agtgaatgaa tttatgggca acatgcaacg tcaaggaatc tcacctgaaa tgtacttcca
     4921 attgactggt acaactcaag aagatttaca taaccaatat tcagctgaag ctgacaagcg
     4981 tgttaaaaca aaccttgtta ttgaagcaat tgctaaagca gaaggttttg aagcaacaga
     5041 tagtgaaatt gaacaagaaa ttaatgacct tgcaacagaa tataacatgc cagctgacca
     5101 agttcgttct cttctttcag cagatatgtt gaaacatgat attgcaatga aaaaagcagt
     5161 tgaagtgatt acaagcacag caagcgttaa gtaatcttat taaggaacaa aacgtacaag
     5221 ccggattaga acgtctttct agtctggttt ttctttttct aaagataagg aattagttaa
     5281 acgttatttt aatgaattag ctttaaagat ttgtcaagaa gaagtgatat gagttgataa
     5341 aagttttgac gaattacttt ttttagcgta aaatggtaga gaatgataaa ataaggagaa
     5401 ctgccttgaa attagacgta tttgcaggac aagaaaaaag cgagctttcc atgattgaag
     5461 tggcgcgtgc cattttggaa gaacgcggtc gcgacaatga gatgtacttt agcgatttag
     5521 tcaatgagat tcaaaattac ttaggaaaat ctgatgcagg tattcgtcat gcattaccat
     5581 ttttctatac tgacttaaat acggatggct catttattcc tctaggtgaa aacaaatggg
     5641 gacttcgttc gtggtatgct attgatgaaa ttgatgaaga aattattact ctagaagaag
     5701 atgaagatgg cgcacaaaaa cgtaagaaaa aacgcgttaa tgctttcatg gatggagatg
     5761 aagacgctat tgattataga gatgatgatc cagaagatga agatttcaca gaagaatcag
     5821 ctgaggttga atatgatgaa gaagatccag atgatgaaaa atcagaagtg gagtcatatg
     5881 attcagaatt gaatgaaatt atccctgaag atgattttga agaagttgat atcaatgaag
     5941 aagacgaaga agatgaagaa gatgaagaac cagttctcta atgtctttag ttcagaaata
     6001 aaaatgtgat tatcatttga caaattgtct attatagttt atattattat tcgggcacct
     6061 ctttatgagg tcaggcaaaa gctctctagt taataggggg ctatttttgt tttctagaat
     6121 aatgccagtt tgcctctata tttagaaaac taaggtgata cctaaccaat gtggaaccaa
     6181 ctaataggga attaacaaga aagcaatgct tttattcagc ttatataaag gagtactatg
     6241 acgaaataca tttttgtaac tggtggtgtt gtatcatcaa ttggcaaagg aattgttgcg
     6301 gcaagtcttg gccgattgtt aaaaaatcgt ggacttaagg tgacgattca aaaatttgat
     6361 ccttatatta atattgatcc agggacgatg agcccctatc agcatggaga agtatatgtg
     6421 acagatgacg gtgctgaaac cgacttggat ttgggtcact atgaacgttt tattgatatc
     6481 aatcttaata aatattccaa tgttacaaca ggtaaaattt atagtgaagt ccttcgtaaa
     6541 gaacgtaaag gtgagtacct tggagcgact gttcaggtga ttcctcatat cacagatgcc
     6601 cttaaagaaa aaatcaaacg cgcagcttca acaacggact ctgatgtaat tattactgag
     6661 gtcgggggga ctgttggcga tattgaaagt ttaccgttct tagaagctct tcgtcagatg
     6721 aaagctgatg ttggttctga aaatgtgatg tatattcaca ccaccttgtt gccatattta
     6781 aaggcagcgg gtgaaatgaa aactaagcca acgcaacatt ccgttaaaga attgcgtggt
     6841 ttagggattc aaccgaatat gttggttatt cgtacggaag agccagttga gcaggggatt
     6901 aaaaataaat tggctcagtt ctgtgatgtg aattcagaag ctgttatcga gtcacgtaat
     6961 gtagaacact tgtatcaaat tccattgaac ttgcaagccc aatcaatgga tcagattgtt
     7021 tgtgatcatt tgaaattgaa tgcgccacaa gctgatatga ctgagtggtc agctatggtt
     7081 gacaaggtga tgaatttaag aaaaacaaca aaaattgcct tggttggtaa atatgtggaa
     7141 ttgccagatg cctacttatc tgttgtggaa gccttgaaac attctggtta tgccaatgat
     7201 accgcaattg acctaaaatg ggtcaatgct aacgatgtga cggtggataa tgcagctgac
     7261 ttgttgggag atgcggatgg gattattgta ccaggtggtt ttggtcaacg tggtacagaa
     7321 ggcaagatcc aagcgattcg ctatgcccgc gagaatgatg tgccaatgct tggtatctgt
     7381 cttggcatgc aactgacttg tgttgaattt gctcgccatg tcctcaatat ggaaggtgca
     7441 aactcttttg aattagagcc aagtactaaa taccccatca ttgatatcat gcgtgatcaa
     7501 attgatattg aagatatggg aggtacgctt cgtctgggat tgtacccatg taaattgaaa
     7561 ccaggttcaa aagcagcaat ggcttacaat aatcaagagg tggtgcaacg tcgtcatcgt
     7621 caccgttatg agttcaacaa caaattccgt ccagaatttg aagcagctgg ttttgttttc
     7681 tcaggggtat cacctgacaa ccgcttagtt gaaattgtag aattgaaaga gaaaaaattc
     7741 tttgtagcag ctcaatacca cccagaattg caaagccgtc caaatcgtcc tgaggagcta
     7801 tacaccgctt ttgttacagc tgctattaaa aatagcaatt agtggtttta agggtaagtt
     7861 tgtggcattc tacttggctg gcagatactg ataaagatta ctagctatta gagggatctt
     7921 ttctataaaa atagcattag aaaaagaaaa acgtgatatt tttaggttag cactttcttt
     7981 ttcagtcatt tttttgtaaa atacttggta tgaaaactat tcgaatagca aagtatttag
     8041 gaattctatt ccttttgata actcttatta gcgtaggtgc tagtttttat ttctttcatg
     8101 ttgcacaaat aagagaagag aaatcgttta ttaataataa gaaacggagt acaaataatc
     8161 cattataccc agctgaacag tcttttgacg ctttacctta cgaaaaacgt caactaacaa
     8221 atcgtgggtt aaaacaagtg gggtggtact taccagctgc tcaaaaaaca aaaaagacag
     8281 ctattgttgt tcatggtttt acgaatgaca aagaagatat gaagccatat gccatgcttt
     8341 ttcatgattt gggctataat gtcttaatgc cagacaatga ggcccatggg gaaagtgaag
     8401 ggaacttgat tggttatggc tggaatgacc gccttaatgt catggcttgg acagaccaac
     8461 tg
//
LOCUS       DAUVUY010000082         8338 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.082, whole genome shotgun sequence.
ACCESSION   DAUVUY010000082 DAUVUY010000000
VERSION     DAUVUY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.082"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..953
                     /locus_tag="VOX58_001571"
     CDS             <1..953
                     /locus_tag="VOX58_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/betaine ABC transporter permease"
                     /protein_id="HES5878790.1"
                     /translation="LALVILAIVLDRMTQLFNSKPQEKAKAGKTNKWIGLAALAVFLI
                     AALGRGIMAMTSGMADKGETVNIAYVQWDSEVASTHVIAEVLKNEGYHVTLTPLDNAV
                     MWQTVANGNADFSTSAWLPVTHGQQYQKYKSKLDDLGPNLKGTKLGLAVPKYMTDVNS
                     IEDLSKQADQKITGIEPGAGIMAAAQKTLKEYHNLSSWELVAASTGAMTTSLDQAIKK
                     KDPIVVTAWSPHWMFAKYDLKYLKDPKETFGSTENINTIARKGLKKDLPNVYKIIDKF
                     HWTQKDMEAVMLDINKGMSPEAAAKKWVEANQSKVSSWTK"
     gene            1084..3726
                     /gene="polA"
                     /locus_tag="VOX58_001572"
     CDS             1084..3726
                     /gene="polA"
                     /locus_tag="VOX58_001572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HES5878791.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKASKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAAISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     CEAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            3913..4368
                     /locus_tag="VOX58_001573"
     CDS             3913..4368
                     /locus_tag="VOX58_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HES5878792.1"
                     /translation="MIYSFQNPSEDILKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEVQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNL"
     gene            4420..4887
                     /gene="perR"
                     /locus_tag="VOX58_001574"
     CDS             4420..4887
                     /gene="perR"
                     /locus_tag="VOX58_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HES5878793.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQSDF"
     gene            5044..5343
                     /locus_tag="VOX58_001575"
     CDS             5044..5343
                     /locus_tag="VOX58_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878794.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     INFDDLRLTMVDMVSLSSEYDVIVTDMILEQTMDSEILFFNQMAPSVVANRLTDM"
     gene            5565..6899
                     /locus_tag="VOX58_001576"
     CDS             5565..6899
                     /locus_tag="VOX58_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HES5878795.1"
                     /translation="MKKYLNQNVYDALIERLHFLFNEFPIVYISFSGGKDSGLLLNIL
                     LDFRDKYYPDREIGVFHQDFEAQYSLTTKYVQETFTSLEGRKKVSLYWVCLPMATRTA
                     LSSYEMFWYPWDDKTEDIWVRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGRWYK
                     QIHGNQKTVCILGNRASESLHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDWSVE
                     DIWHAYYKFSYSYNELYDLFYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETWVKL
                     LGRVQGVNFSNIYGRTKAMGYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKFNKS
                     IDFWHKTGGGLAEETINELIEKGYRIARNGISNYTSFKHSRVIFLDQIPDDTDDIVTT
                     KDIPSWKRMCFCILKNDHICRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
     gene            6892..7422
                     /locus_tag="VOX58_001577"
     CDS             6892..7422
                     /locus_tag="VOX58_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HES5878796.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(7469..7624)
                     /locus_tag="VOX58_001578"
                     /pseudo
     CDS             complement(7469..7624)
                     /locus_tag="VOX58_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7751..7978)
                     /locus_tag="VOX58_001579"
                     /pseudo
     CDS             complement(7751..7978)
                     /locus_tag="VOX58_001579"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8006..8239)
                     /locus_tag="VOX58_001580"
                     /pseudo
     CDS             complement(8006..8239)
                     /locus_tag="VOX58_001580"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         2633 a         1435 c         1763 g         2507 t
ORIGIN      
        1 gtcttgcctt ggttatcttg gcgattgtgc ttgaccgtat gacacagttg ttcaatagca
       61 aacctcaaga aaaagccaaa gctggcaaaa ccaataaatg gattggtcta gcagcccttg
      121 ctgtcttttt gatagctgct ttaggacgcg gtattatggc tatgacttct ggtatggcag
      181 ataagggtga aactgttaac atcgcttacg tgcagtggga ttctgaggtt gcctcaactc
      241 atgtgattgc agaagttttg aaaaacgaag gttaccatgt gaccttgact ccgcttgaca
      301 atgccgttat gtggcaaacc gttgcaaatg ggaacgctga tttttcaact agtgcttggt
      361 tgccagtcac ccatggccaa caatatcaga aatacaagtc aaaattagat gatttaggac
      421 ctaacctaaa agggaccaaa cttggtttag ctgttccaaa atacatgaca gatgttaata
      481 gtattgagga cttaagcaaa caagccgatc aaaaaatcac agggatcgaa ccaggtgcag
      541 gtatcatggc agcagcccaa aagactctaa aagagtatca taatctatct agttgggagt
      601 tagtggcggc atcaacagga gccatgacga cttccttgga tcaagcgatc aagaaaaaag
      661 atcctattgt cgtgacagct tggtcaccac actggatgtt tgccaagtat gacttgaagt
      721 accttaaaga tcctaaagaa acctttggct cgactgagaa catcaatacc attgctcgta
      781 aaggcttgaa aaaagatctt ccaaatgtct acaaaatcat tgataagttc cactggaccc
      841 aaaaagatat ggaagcagtc atgcttgaca tcaacaaagg catgagccca gaagctgccg
      901 ccaaaaaatg ggtagaggca aaccaatcta aagtatcaag ttggacaaaa taagtgcttt
      961 tgtctaaata gcaaatagag aagtctagga attttcctag gctttttagg ttaactattt
     1021 acttatcagc aaaaccaatg gcttaaagga gaaaagcccg tccctctgtg ttataataag
     1081 gctatggaaa acaagaataa attactatta attgatggct catctgttgc ctttcgtgcc
     1141 ttttttgccc tctacaatca aattgaccgc tttaaaaatc atagcggtct tcataccaat
     1201 gccatttatg gctttcattt gatgctagac cacatgatga aaagagtcca gcctactcac
     1261 gtgctggtgg cttttgatgc gggcaaaacc accttccgaa cagaaatgta tgccgattat
     1321 aaggcgggtc gcgccaagac accagaggaa tttcgtgagc agtttcctta tattcgtgag
     1381 atgttgacag cccttgggat tgcttattat gagttggaac actacgaagc agacgatatt
     1441 atcgggacgc ttgataaaat ggcggagcgc acagaagtgc cttttgatgt gaccattgtc
     1501 agtggtgata aagatttgat ccagttaact gacgaaaata ccgtggttga aatttctaaa
     1561 aaaggggttg ccgaatttga agaattcaca ccggcttacc tcatggaaaa aatgggcttg
     1621 acgccaaacc agtttattga cctcaaagcc ttgatgggag ataaatcgga taatattcca
     1681 ggagtgacca aaatcggtga aaaaactggg ctcaagctcc ttcatgaatt tggtagtttg
     1741 gaaggcattt acgagcatat tgatggattc aaggcctcaa aaatgaaaga aaacttgatc
     1801 aatgaccgtg accaagcttt cctatctaaa acgctcgcca ctatcaatac agcatctcct
     1861 atcacaattg gcttagatga tattgtctac aatggtccag atgttgctag cctctctcaa
     1921 ttttacgatg agatggattt tgtgcaactc aagaaaggtc ttgctagcca gatgcctcaa
     1981 gagccagtag ctgccatttc ctatcaagaa gtgaccaatg tgtctgctga tttgtttagc
     2041 gcagaagaca tcttctactt tgaaacgctt cgggataatt accattgtga ggccatcatt
     2101 ggttttgctt ggggacatgg tgagcagatt tacgcatcca ctgaccttag cctattagcg
     2161 acggatagtt ttaagcaggt cttccaaaaa cccattgcca cttatgattt taaacgtagc
     2221 aaggtcttgt tgagccactt aggaattgag ttggtagcac ctagctatga tgctagatta
     2281 gctaattacc ttctgtcaac tgtagaagac aatgagctta gcaccattgc ccgcattttt
     2341 actgacatct ctttggaaga agacgataca gtctatggta aaggtgctaa acgggctgtt
     2401 cctgataaag acgttttact agaacactta gctcgtaagg tcaaagtatt attagatagc
     2461 aagtctcaaa tgctagacaa gcttactgcc catgagcaac tagacctcta tcagaatatt
     2521 gaattgcctt tggctaatgt ccttgctaaa atggaaattg aagggatcaa ggttaatagg
     2581 gcgactttgc aagatatggc agagcaaaac aaggtcatta ttgaagcgct cacccaagag
     2641 atttatgata tggctggcca agaatttaac attaattcgc ctaaacagct gggcagtatc
     2701 ttgtttgaga agatgcaact acctcttgag atgactaaga agaccaaaac aggttattct
     2761 acagctgttg atgttttgga acgtttggca ccgattgctc caattgtggc taaaattttg
     2821 gattaccgcc agattactaa attgcaatcc acctatgtga ttggtttaca ggattatatt
     2881 ctggctgatg gcaagattca tacccgctat gtgcaggatc tcacccagac agggcgtttg
     2941 tctagtgtgg accctaatct tcaaaacatt cccattcgtt tggaacaggg gcgtttgatt
     3001 cgtaaggcct ttaccccatc tcatgaagat gccgtcttgt tgagttcaga ttactcacag
     3061 attgaattgc gggtactggc acatatttca ggggatgagc atttgattgc tgcctttaat
     3121 gaaggggcag acattcatac ctcaacggct atgcgtgtgt ttgggattga caaggcagca
     3181 gatgttactg ccaatgaccg ccgtaatgcc aaggctgtta actttggaat tgtgtatggg
     3241 atttctgatt ttggtttgtc aaataacctt ggtatcaccc gtaaacaggc caagtcttac
     3301 attgatacct attttgaacg ctatcctggg attaaagcct atatggaaaa tgtggtgcgt
     3361 gaggctaaag acaagggcta tgtagagacc ctctttaagc gtcgtcgtga attaccggat
     3421 atcaactctc gtaatttcaa cgttcgctct tttgcggagc gtacagccat caactcgccc
     3481 attcaaggaa gcgcagctga tattctcaaa atcgctatga ttaatctgga taaggccttg
     3541 caagcaggtg gttttagagc taaaatgctg cttcaagtgc atgatgaaat cgttttggaa
     3601 gtgccaaatg atgagttgac tgctataaaa aaacttgtca aagacaccat ggaagctgct
     3661 gttgatttag ctgttccttt atgtgtcgat gaaagtactg ggcagagctg gtacgaagcc
     3721 aaataagttt tcttacttgt tatttctcat tttgacaact tatgttataa taaactaaaa
     3781 aattgtagga gaagtcatga agctgctttg agctttttga cggataaggt taaaagtgat
     3841 tgaagccatg ctttaggagt tatcctgcta tattgaacag ctgtagaggt tattaaatag
     3901 ataggggaac aagtgattta ttcgtttcaa aatccctcag aggatatcct aaaagcttac
     3961 ttggagtcag ccaaaaccat tgcggttgtt ggtctttctg accgcaaaga cacagcagct
     4021 tatggtgtcg ccaaatttat gcaggctatg gattatcgga tcattccagt caatcccaaa
     4081 ttagcaggtc agcttatttt gggagagaag gtctatgcca gtatcaaggc aattcccttt
     4141 gaggttgaca ttgtagatgt ctttcgcaga agtgagtttt taccagaagt tgccagagac
     4201 tttttagcag gtcaggccaa agtcttttgg gcgcaattag gccttgaaaa ccaagaagta
     4261 caaaccattt tacggtcggc aggcaaagag gctattgtga tgaatcgctg cctaaaaata
     4321 gactatttgc aattagtcgc taaaccagaa tgcatcacaa atctttgaaa ctagagacgt
     4381 gcgtgataat aaaggaaaaa aggataggag tagcagctta tggacattca ttcacatcag
     4441 caagccctag atgcctatga aaatgtccta gagcatctac gggaaaaaca tattcgaatt
     4501 acagagacac gtaaagccat tatttcttac atgattcagt ccacagaaca ccctagtgcc
     4561 gataagattt accgtgactt acagcctaac tttcctaaca tgagccttgc cacagtctat
     4621 aataatttga aagttttagt tgatgaaggt tttgtctcag agttgaaaat cagcaatgat
     4681 ttaaccacct actatgactt tatgggccat caacacgtca atgtggtttg tgaaatatgt
     4741 gggaagattg ctgactttat ggatgttgat gttatggata ttgccaaaga agcccatgag
     4801 caaacaggtt ataaggtcac gcgtatccca gttattgctt acgggatttg tccggattgc
     4861 caagcaaaag accaatctga tttttagagt taacccttaa ataaaggcat tataaatata
     4921 gtgcctttac aaatcaaaaa cccccagttc aataccagac ctttgttgct tatgttaaag
     4981 agttatttgc tggggatcat aaggaattaa aggaaagaac catcaatcat ctcctctatt
     5041 tgtttgttcc tcacctggga aaatctcttt cttacaaatc aatcgcgtcg tcgcttgaaa
     5101 ctattagtgg ttgagagaag ttataacaat gttggcaact tcttaaaaga gtattttgga
     5161 ggattttttg agattatcaa ctttgatgac ttgaggctga ccatggttga tatggtgtca
     5221 ctgtcttcag agtatgacgt aattgtgaca gatatgattc ttgagcaaac aatggactcc
     5281 gaaattttat ttttcaatca gatggcccct agtgtggtgg cgaatagatt gacagacatg
     5341 taactaaaca atgataatat ctgtgagagc atcggaattt aaaaaatgag cgataaataa
     5401 gattaagaat aagttagact tacaaggagt agagtctatt accttgaaaa agaaaagcaa
     5461 ggtttaatta ttcaacttga aaaattaaaa aacaaaaagc tcgaaaaaga gattcttagt
     5521 gaatctatgt attaatattt ttaaatgcat aatgaggaag tttagtgaaa aagtatttaa
     5581 atcaaaatgt ttatgatgca ttgatagaaa gattacattt tttgtttaat gaatttccta
     5641 ttgtctatat ttccttctca ggtggaaaag atagtgggtt acttcttaat attttactgg
     5701 attttagaga taaatattat ccggatagag aaataggtgt gtttcatcaa gattttgaag
     5761 cacagtacag tttaacaaca aaatatgttc aagaaacatt cacttcttta gaaggtagaa
     5821 aaaaagtttc tttgtattgg gtgtgcttac cgatggctac tagaactgca cttagtagtt
     5881 atgaaatgtt ttggtatccg tgggatgaca aaacagaaga tatctgggta agaccaatgc
     5941 catctcaaga ttatgttatt aatttggaaa ataattcaat tactacttat cgttataaaa
     6001 tgaatcagga ggatttagcg aaacaatttg gtaggtggta taaacaaatt cacggaaatc
     6061 aaaaaacagt ttgtatatta ggaaaccgtg cttcagaatc cttacacaga tatagtggtt
     6121 ttatcaataa aaagtatggt tatcaaaagg aatgctggat taccaaacaa ttcaaagatg
     6181 tctggacagc atctccctta tatgattggt cagtggaaga catatggcat gcttactata
     6241 agttttctta ttcttataac gaactttatg atctattcta taaagcgggt ttaaaaccat
     6301 cacaaatgag agttgcatct ccttttcaag actatgctgt tgatagccta aatctttata
     6361 gaataataga tcaagaaact tgggtaaagc ttttgggacg tgttcaagga gttaactttt
     6421 cgaatattta tggacgtact aaagcaatgg gatataaatc aattgcttta ccaaagggtc
     6481 attcgtggaa gtcgtacact cagtttcttt tatcaacgct accagtgagg ttaagaaata
     6541 attatgttag aaaatttaat aaatcaatag acttttggca taaaacaggc ggaggacttg
     6601 cggaggaaac aattaatgaa ctaattgaaa aaggatatcg tattgctaga aatggtattt
     6661 caaattatac ctcttttaaa cattcaaggg ttattttttt agatcaaatt cctgatgata
     6721 cagacgatat tgtaacaaca aaggatatac caagctggaa acgaatgtgt ttctgtattt
     6781 taaaaaacga tcatatttgt cgaactatgg ggtttggttt gacaagagag caacaaaaaa
     6841 tagtagcctt tttaaaagag aaatataaga cgagtttaaa ggagttagaa agtgagtaat
     6901 tttattagtc cagtctatga gattaaatca attccaattg aaaagatttc tccaaatgat
     6961 tataatccga attcagttgc tccgcctgaa atgaaattat tgtatgatag tataaaatct
     7021 gatggttata ctatgccgat tgtttgctat tacgataagg aagaggatcg gtatagtatt
     7081 gttgatggtt ttcatagata tcgtataatg ctagattatt cagatattta tgaaagggaa
     7141 agtgggagat tacctgtttc tgttattgat aagtcattag actatcgaat ggcttctacc
     7201 attcgacata atagagctag aggaagtcat gatgttgatt tgatgagtca gattgtaaag
     7261 gatcttcatg agtgtgggcg aagtgataac tggattgcaa aacatttagg aatggataaa
     7321 gatgaaattt taagactgaa acaaataaca ggattggctt ctctttttaa ggaccatgag
     7381 tttaatcaat cttgggaacc agaagagcta ttaaatgagt aaacaggaag taagagactc
     7441 attgtcaact gtagtgggtg acttatcatt aacaacgaga gagaatcaga ttggttctct
     7501 ctttttggat gttcaaagcg atgaggattt tggttttaaa gttgtcaaag ttctgaaatc
     7561 taaaggcatt gtacttaatg cccttgatga gtctgtttgt ggcttcaagt ttgtcgttga
     7621 atgaggcagc tggagggcag cggtgacgta tttttgtagc ggacagagga ttgagagctg
     7681 ttttgaaagt atgtgtataa ggggacttca taccctggac ttgtcaagaa aaaatagaca
     7741 taaagttaag ctaatgagta actatttttt caaattctac aggtgagaga taccctaaag
     7801 ctgaatgcag acgtttaggg ttatagtaag tctcactata tttaaaaatc tcaagctgag
     7861 cttgctcaat agttgcgaaa tgagcatcat tgacaagctc tctttttaat gttttataaa
     7921 aggattccat gagtgcatta tcgtatggat taccttttcg actcatcctg gacttacatt
     7981 ttttctgtct aagtaggtcc tgaaatcatg ctccggtata ttgagaaccc tgatctgtat
     8041 ggacgattac cccttcttta ggcttttctc tattataagc ttgattgaaa gcttctgtta
     8101 ccaacttgtc ttgcatacgt cggcccattg cccaaccaac aatctttcta ctataaacgt
     8161 ctatcaagac agacacataa agtattcctt cttgtgtagg aatgtaggtt aagtctccta
     8221 gccataattt attcttatca gtcgcttgga aacattgatt aacaagattg ggacgagttc
     8281 atgaagaacg tcgacggtta tagtatttat attgatatcg acttccttta gcatagag
//
LOCUS       DAUVUY010000083         8239 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.083, whole genome shotgun sequence.
ACCESSION   DAUVUY010000083 DAUVUY010000000
VERSION     DAUVUY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.083"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..189
                     /locus_tag="VOX58_001581"
     CDS             <1..189
                     /locus_tag="VOX58_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878797.1"
                     /translation="TQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEELML
                     FGERLSEIKIRKNNSVFK"
     gene            351..623
                     /locus_tag="VOX58_001582"
     CDS             351..623
                     /locus_tag="VOX58_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HES5878798.1"
                     /translation="MRLDKYLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNIKLN
                     DHIEISFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITLNEEA"
     gene            610..981
                     /locus_tag="VOX58_001583"
     CDS             610..981
                     /locus_tag="VOX58_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HES5878799.1"
                     /translation="MKKPSIVQLNNHYIKKENLKKKFEEEESQKRNRFMGWILVSMMF
                     LFILPTYNLVKSYVDFEKQNQQVVKLKKEYNELSKRTKKEKQLAERLKDDNFVKKYAR
                     AKYYLSREGEMIYPIPGLLPK"
     gene            1116..2402
                     /locus_tag="VOX58_001584"
     CDS             1116..2402
                     /locus_tag="VOX58_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HES5878800.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQLPSNPNLYQETCAYKDSDLTLPAGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFRSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYEDITAIADDVYGILK"
     gene            2399..3685
                     /gene="tilS"
                     /locus_tag="VOX58_001585"
     CDS             2399..3685
                     /gene="tilS"
                     /locus_tag="VOX58_001585"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HES5878801.1"
                     /translation="MMTYQEIFNEIKNKAYFKNHRHVLIAVSGGVDSMNLLHFLYLFQ
                     DKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYA
                     FFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPF
                     LTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNYLPLLKQENPRFIQGLNQLALENSLL
                     FQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQLLQIIQTA
                     KQGYYYLKKDYYIFIDKFSFKITKIVPKTELVKDEKMLEYDSNLCYRDYCFSFMPKSN
                     EDQGQVSIPLFSLSSIKLRSRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQ
                     DEQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG"
     gene            3690..4232
                     /gene="hpt"
                     /locus_tag="VOX58_001586"
     CDS             3690..4232
                     /gene="hpt"
                     /locus_tag="VOX58_001586"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HES5878802.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
     gene            4254..6233
                     /gene="ftsH"
                     /locus_tag="VOX58_001587"
     CDS             4254..6233
                     /gene="ftsH"
                     /locus_tag="VOX58_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HES5878803.1"
                     /translation="MKNNKNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYS
                     KLIKHLKAGDIKSLSYQPSGSIIEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSL
                     VLPSDTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGG
                     GGARGAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFV
                     GADLENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGR
                     VAEEIVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEK
                     SYSAQTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIY
                     ETGKMPVDLETDDNEAHALSYDEIKNKMTESESAE"
     gene            6560..7951
                     /locus_tag="VOX58_001588"
     CDS             6560..7951
                     /locus_tag="VOX58_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HES5878804.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLVFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLAYGYTHSIENK"
BASE COUNT         2945 a         1160 c         1464 g         2670 t
ORIGIN      
        1 actcaggatt atttcgaagc cttatcgaaa acccatctta aagctaaaat tagtgaacat
       61 caggggaaaa tcgatatcgt ctttgacgtt cgccatcaaa aagattatag aattttagaa
      121 gaattgatgt tatttggaga aaggcttagt gagataaaaa tcagaaaaaa caattcagtt
      181 tttaaataat aatttgaaac aacttttaga ctgaatagta ttaatgacaa tggaacgaac
      241 tagctcttta tacttttgtc aaaaatctaa gatctttgct gcgaaattag gatcttttct
      301 accatttaaa aaataaaaat gataaaatga agaggattgt aggagaaatt atgagactag
      361 ataaatatct aaaggtatcg cgccttatta aacgtcgttc agtagcaaaa gaagttgcgg
      421 ataagggacg aattaaagtt aatgggatac ttgctaaaag ttcaacgaat ataaaactaa
      481 atgatcacat tgaaattagt tttggaaata aattactgac agtaagagtc attgaaataa
      541 aagatagtac aaaaaaagaa gatgctctta agatgtatga gataatcagt gaaacaagga
      601 taacattaaa tgaagaagcc tagtattgtt caattaaata atcattatat taagaaagag
      661 aatctcaaaa aaaaatttga agaagaagaa tctcaaaaaa gaaatcgttt tatgggatgg
      721 atccttgtaa gtatgatgtt tttatttatt ttgccaactt ataatcttgt caaaagttat
      781 gttgattttg aaaagcaaaa tcaacaggtg gttaaattaa aaaaagagta taatgaattg
      841 tcaaagagga caaaaaaaga aaaacaatta gcagaacgac taaaagatga taattttgtc
      901 aaaaaatatg ctagggcaaa atactattta tcgcgtgaag gagaaatgat ttatcctatt
      961 ccaggactat taccaaaatg atgatggata atattgtaaa aaaaatagaa gcatttcttg
     1021 ctttttctga taaaaaatta gcagagctgc aacaggaaaa tcaaaaagtt aaagaagaaa
     1081 gtcagtatat tcaaaaaaac tagaaaggag tcaatatgag aaaattatta gcggctatgt
     1141 taatgacttt ttttctgact cctttaccag tgattagtac agaaaaaaaa cttatatttt
     1201 caaaaaatgc tgtttatcaa ttgaaacaag atgtcgttca atcaacacaa ttctataatc
     1261 agctaccctc taatccaaat ctttatcaag aaacgtgtgc ctataaagac agtgatttaa
     1321 ctctaccagc aggaagatta ggtgtaaatc aaccattact tattaaatcg cttgttctta
     1381 acaaagaatc tttaccggtt tttgagttag ctgatggtac ctatgttgag gctaatcgac
     1441 aattgattta tgacgatatt gtacttaatc aagtagatat agatagctat ttttggacac
     1501 aaaagaaact taggctttat tcagcccctt atgttttagg tacgcaaaca attccttctt
     1561 ctttttcatt tgctcaaaaa gttcatgcca ctcaaatggc acaaacaaac catggaactt
     1621 attatcttat tgatgataag ggctgggcat cacaagaaga tctagttcaa tttgataacc
     1681 gcatgttaaa agtccaggaa atgctcttac aaaaatataa taacccaaat tattcaattt
     1741 ttgtaaagca actcaacaca caaacaagtg ctggtattaa tgctgataaa aaaatgtatg
     1801 ctgcaagtat ctcgaagtta gcaccacttt atattgttca aaaacaatta caaaaaaaga
     1861 aattagcaga gaataaaact ttgacttata ctaaagatgt taatcatttt tatggagact
     1921 atgatccatt gggaagtggt aaaattagta aaatagctga taataaagat tatcgtgttg
     1981 aagacctact gaaagctgta gcacaacaat cggataatgt agcaactaat attttaggtt
     2041 attatctatg tcatcagtat gataaagctt tccgctcaga gataaaagct ttatcaggta
     2101 tcgattggga tatggagcag cgcttattaa cttctcgttc agctgcaaat atgatggaag
     2161 ctatttatca tcaaaaaggc caaattattt cttacctttc aaataccgaa tttgatcaac
     2221 aacgtatcac aaaaaatatt actgttccag ttgcacataa aattggtgat gcttatgatt
     2281 ataaacatga cgttgctatt gtttacggta atactccatt tattttgtct atttttacaa
     2341 ataaatcaac ctatgaagat attacggcta ttgcagatga cgtttatggt attttaaaat
     2401 gatgacctat caagaaattt ttaatgaaat aaaaaataaa gcctacttta aaaatcatag
     2461 acatgtgtta atagccgtat ctggtggagt agattcaatg aatttattgc actttttata
     2521 cctgtttcaa gacaaattga aaattcgtat tggtattgct catgttaatc acaaacaaag
     2581 atcagaatca gattcagaag aagcttattt aaagtgttgg gctaaaaaac atgacatacc
     2641 tatttatgtt tctaattttg aaggaatatt ttcagaaaaa gcagcacgtg attggcgcta
     2701 tgcttttttt aaaagtatta tgttaaaaaa taactattca gctttagtaa cagctcacca
     2761 ttcagatgat caagcagaaa ctattttgat gaggctaatt agaggaagcc gattgaggca
     2821 cttgtcgggt ataaaaagcg tgcaaccatt tgccaatggt caacttataa gacctttttt
     2881 aactttctca aaaaaggact tgccagaaat ttttcatttt gaagattctt caaataggga
     2941 attatcattt ttaagaaata gagttagaaa taattattta ccattgttaa agcaggaaaa
     3001 tcctaggttc attcagggat taaatcaatt agcacttgaa aatagcttac tttttcaagc
     3061 ttttaaggag ctaacaaatc atattactac cactgattta acagaattta atgagcaatc
     3121 taagtctatt caatactttt tgttacaaga ttatttagaa ggttttccag atttagattt
     3181 aaaaaaatct caatttactc aattattgca aattatccaa actgctaaac aaggctatta
     3241 ttatctcaaa aaagactatt atatattcat tgataaattc tcttttaaaa ttacaaaaat
     3301 cgttcctaag actgagttag ttaaagatga aaaaatgtta gaatacgata gtaacttatg
     3361 ctacagagat tattgttttt catttatgcc aaaaagtaac gaagatcaag gacaggtaag
     3421 tataccttta tttagtttat cgtctattaa attaagaagt cgccaatctg gagattatat
     3481 ttcttttggt cacttttcaa aaaaaataag acgtttattt atagatgaaa aattcacaat
     3541 agctgaacgc caaaatgcta ttattggaga gcaagatgag cagattatat ttgtattgat
     3601 aggtaataaa acttatttga gaaaagcttg taaacatgat ataatgttgg ctaaactgta
     3661 tattgataaa ttagaaaaag ggtgacagca tgctcgagca agatattcaa aaaattcttt
     3721 actctgaaaa tgatattatt cggaaaacta aaaaattagg agaacaatta acaaaagatt
     3781 atcaagaaaa aaatccgctt atgattggtg tattaaaagg atcagttcct tttatggcag
     3841 aactaatgaa acacatcgat actcatgttg aaattgattt tatggtagta tcaagttatc
     3901 atggcggtac atctagcagt ggtgaagtta aaattttaaa agatgtagat accaatattg
     3961 aaggaagaga tatcataatt gttgaagata ttattgacac cggtcgcacc ttaaaatatt
     4021 tacgagatat gtttaagtac cgtaaagcta atactatcaa aattgcaaca ctgtttgata
     4081 aacctgaagg tcgtgttgtt aaaattgaag cagattatgt ttgttacaat attccaaatg
     4141 aatttatcgt aggctttggt ttagattacg cagaaaatta tcgcaatctt ccttacgtag
     4201 gtgtattaaa agaagaagtc tattcaaaat agaaaaaggt caatgtttac tgtatgaaaa
     4261 ataataaaaa taatggtttt gttaaaaatt cttttatata tatattgatg attattgttg
     4321 tcatcacagg atttcaattt tatcttaagg gaaccagcac acaaagccaa cagattagtt
     4381 attccaaatt aattaaacat cttaaggctg gagatataaa atctttaagt tatcagccta
     4441 gcggtagtat tattgaagtt aaagggaaat atgagaaacc tcaaaaagtg acagttaatt
     4501 caggactatc atttttaggt ggaagagctt caacacaggt aacagagttc tcatctttag
     4561 tattacctag tgataccatc ttgaaagaaa tgacagcagc agctgataaa aatggaacag
     4621 agcttacagt taagcaggaa agttcaagtg gtacttggat taccttctta atgagctttt
     4681 tacctattgt tatttttgct gctttcatga tgatgatgat gaatcaaggt ggtggaggag
     4741 ctcgtggtgc tatgagtttc ggcaaaaata aggcaaaatc tcaatcaaaa ggcgatgtta
     4801 aagtgagatt tacagacgtt gctggtgccg aagaagaaaa acaagaatta gtagaagttg
     4861 ttgatttttt gaaaaatcct aaaaaatata aatcactagg agcacggatc ccagcgggag
     4921 ttcttctaga ggggcctcca ggtactggta aaacattact agctaaagct gtagcaggtg
     4981 aagctggagt gccatttttc agtatttcag gttcagattt tgtcgaaatg tttgtcggtg
     5041 taggtgctag tcgtgttcgt tctttatttg aggatgctaa aaaagctgag cgtgcaatca
     5101 tctttatcga tgaaattgac gctgtaggtc gtcgtcgtgg tgctggcatg ggaggcggta
     5161 acgatgaacg agaacagacc cttaaccaat tactcattga aatggatggt tttgagggta
     5221 atgagaacat tatcgttatt gctgcaacta accgtagtga tgttttagat ccagctttac
     5281 taagaccagg tcgttttgat cgtaaggttt tagttggtcg ccctgatgtt aaaggacgtg
     5341 aagccatttt acgtgttcat gctaaaaata aaccacttgc aaatgacgtt aatttgaaag
     5401 tagtagccca acagactcca ggttttgtag gtgctgatct tgaaaatgtt ttaaatgaag
     5461 ctgcgttggt tgctgctcgc agaaataaaa ttaaaattga cgctagtgat attgatgaag
     5521 ctgaagatcg tgttattgct ggaccatcaa agaaagatcg cactatttcg caaaaagagc
     5581 gagaaatggt tgcttatcac gaagctggtc atactattgt tggtttagtt ttatctaatg
     5641 cgcgtgtagt tcacaaggta actattgtac ctcgtggacg agcaggtggt tatatgatcg
     5701 ctcttccaaa agaagatcaa atgcttcttt ctaaagaaga tctgaaagag caattagcag
     5761 gattgatggg tggtcgtgtt gctgaagaaa ttgtttttaa tgctcaaaca tcaggtgcgt
     5821 caaatgactt tgagcaagct actcagatag caagggccat ggttacggaa tatggtatga
     5881 gtgaaaagct tggaccagtt caatatgaag gtaaccatgc gatgatgcca ggacagatct
     5941 ctcctgaaaa atcttactct gcccaaacag ctcaaatgat tgatgatgag gttcgtgaac
     6001 tcttaaatca agctcgtaat caagcagctg atatcatcaa tgagaatcga gatactcata
     6061 aattgattgc tgaagctctt ctcaaatatg aaactctaga cgctgcacaa attaaatcaa
     6121 tttatgaaac tggtaaaatg ccagttgatt tagaaactga tgataatgaa gctcatgcgt
     6181 tatcttatga cgaaattaaa aataaaatga cagaatctga atctgcagaa tgatataaaa
     6241 aagctgaact tatagattaa gttcagcttt tttatcaaca caatatcctt gtaaacgtta
     6301 tgaattgtaa taggtgccta attgctagaa atgagaagat gacatacttt tttttcttta
     6361 ttacttaaag tgagaaagac tttggtattt aaaactagaa aattgttaaa gccatttgaa
     6421 aaaatctatt agtgttttct tagaataata gaagaacaga agatagcaaa agtttcagtc
     6481 aaccaataag atgaaattta caatcattac taactatgat acacttagta gaaaatcatt
     6541 tactatagaa ggtgataaca tgactatttt tcgtaaaaaa aagaaatatt ccaataaaac
     6601 agaaatgcaa aggcacttca aagttattga tttagttttt ttaggcctag gttcaatggt
     6661 tggaacaggt atctttacag ttactggcat aggcgctgct aagtatgcag gtcctgcttt
     6721 aacaatatcg attatcattt ccgcgattgc tattggaatt ttagccttat tttatgcaga
     6781 atttgcgtct cgtatgcctt ctaacggtgg tgcttatagt tatgtttatg caaccttggg
     6841 agagtttcca gcatggcttg ttggctggta tattattatg gaatttttaa cagctatatc
     6901 tagtgttgct gttggctggg gaagttattt aaaaggatta cttgccaatt ataatatagt
     6961 attaccaaac gctttaaatg gcacgttcaa tcttaagaat ggaacttaca tagatattct
     7021 tcctgtttta gtgatgtttt tcgtgacagg gattgtccta atgaattcga aattagccct
     7081 gagattcaat agttttttag ttattttaaa attttcagca ctggctttat ttatctttgt
     7141 aggcattttt tttattgatc acaataattg gtctcatttt gctccatatg gtgtaggaca
     7201 aattactgga ggtaaaacag gtatctttgc tggtgcttct gttatgttct ttgcattttt
     7261 aggatttgaa tctatttcaa tggcagttga tgaagtgaaa gaaccacaaa aaacaattcc
     7321 taaaggtatc attttatctt tgattattgt aacagcactc tatatagttg tcaccactat
     7381 tttaacggga attgtacact acactaaatt aaatgttcca gacgcggtgg cttttgcttt
     7441 aagaaatatt agactttatt gggcagctga ctatgtgtcg atagttgcta ttttgactct
     7501 tattacggta tgtatttcta tgacctatgc attagctaga acaatttaca gtattagtag
     7561 agatggttta ttacctaaat cactttatac tctcactaaa aaaaataaag tgcctcaaaa
     7621 tgctactctt gtcactggac tacttgctat gatttgtgcg ggaatatttc ctttatcgag
     7681 tctagcagag tttgttaata tttgtacttt agcatattta attattctgt cgggtgcaat
     7741 tattaaactc agaagaatag aaggagaacc aaaagcaaat gaatttaaaa ctcctctagt
     7801 accattccta ccaatgttgg caattatcat ttgtctatca tttatgagcc aatataaagc
     7861 ttttacttgg attgcttttg ctattgccac aattattggg acattaatct acttagctta
     7921 tggttatact cattcaatag aaaataaata atgttttgat aataagattt gacattacat
     7981 tatagactat aatgtttaga agtaaatgag tagtaaaatc aatacctact tacttatcaa
     8041 agttgtaata tttaaaatta cccaacccct agagaattga ttagtctcta ggggttgatt
     8101 ggtacaatga catagactct aaaacgattt taatactaaa tatgatagat ataactttca
     8161 aaaaagaaaa ttagttcatt tttgttacac aataaaattt gttatttagc agtaatataa
     8221 ttgaaaaaca gaaacattt
//
LOCUS       DAUVUY010000084         8138 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.084, whole genome shotgun sequence.
ACCESSION   DAUVUY010000084 DAUVUY010000000
VERSION     DAUVUY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.084"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..1435)
                     /locus_tag="VOX58_001589"
     CDS             complement(<1..1435)
                     /locus_tag="VOX58_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HES5878805.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNTA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNFWIEDDSWLVPTDI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNK"
     gene            complement(1470..2963)
                     /gene="malQ"
                     /locus_tag="VOX58_001590"
     CDS             complement(1470..2963)
                     /gene="malQ"
                     /locus_tag="VOX58_001590"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HES5878806.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEAADLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            complement(3079..4098)
                     /locus_tag="VOX58_001591"
     CDS             complement(3079..4098)
                     /locus_tag="VOX58_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HES5878807.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDIKVPDDMSIITF
                     NNSNYAKLIHPYLTTFDINVENLGKMSFKQLLDIINSNEQNLSQTIFVPFSLKQRESV
                     RDISH"
     gene            4341..5588
                     /locus_tag="VOX58_001592"
     CDS             4341..5588
                     /locus_tag="VOX58_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HES5878808.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQILFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            5862..7223
                     /locus_tag="VOX58_001593"
     CDS             5862..7223
                     /locus_tag="VOX58_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HES5878809.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPNFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINAKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            7223..8059
                     /locus_tag="VOX58_001594"
     CDS             7223..8059
                     /locus_tag="VOX58_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HES5878810.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
BASE COUNT         2500 a         1601 c         1363 g         2674 t
ORIGIN      
        1 ctttattgtc tttaaggtaa cgtttaagag ctaatttgtt gttgaatttg atttcagcca
       61 atttagcatg aacagctttg tcatctgcaa aggccatcaa tttttctaat tttgttgcgt
      121 cagtcaagta ctcatcgcca ataagttctt taatgtaatc agccaagtct tggttagcaa
      181 attctagcca acgacggaaa gtaatcccgt tggttttgtt gttgaatttt tctgggtaaa
      241 ggtcatagaa agcttttaat tcgctgtttt tcaagatttc tgtgtggagt gctgctaccc
      301 cattgacact tgttgcaaaa tgaatatcca tatgggccat gtgcacacga cctgattcgt
      361 caataatttg aacagctgga tcagacactt ctgaacgaac aagagcatcc aatttttcaa
      421 tgatggttac caagtgtggc actacttcgt ttaagtaagc agttggccat ttttcaaggg
      481 cttctgcaag aatagtgtgg ttagtgtaac caaccatatt tttcacaaca gctaccgctt
      541 catcaaagtc aaagccatgt ttttcagtca agagacgaat taattctggg atgaccattg
      601 atggatgcgt gtcattgatt tgcacgtaag cgtagtctgc aaggtcatga aggtttgaac
      661 cacgttcgat agcttcatca atgattaatt gcgctgcatt tgacaccata aagtactgtt
      721 ggtaaatacg gagcaattcc ccatttttgt cagaatcgtc tgggtagagg aacaaggtca
      781 agtttttagc aatttgcgtt ttatcaaatg aaataccgtc tttgattaac ccgtaatcaa
      841 cgccctcgat atcaaacaag ttaaggtagt ttttagtgtc gcgtttgtaa cccaaaacat
      901 caatacgatc aagacgagat ttcaaggtga agttcttaaa aggcacatcg taagaaatgt
      961 ctgttggaac caaccatgag tcatcttcaa tccagaagtt tggctcagct tcttgctcat
     1021 tgtgtttgaa gacttgctta aacaacccac aatgatagtt taaaccaacc ccttcaccat
     1081 taatcccaag agatgaaata gagtcaatga aacatgaagc aaggcgtcct aaaccaccat
     1141 tacctagtga tggttctaat tcaacatctt cgacttctgc gatagatttg ccagcagctg
     1201 ccaattcttc tttgatatct ttataaattc ctaggttaat caggttgttt gatagtaatt
     1261 taccaatcaa aaattccgct gagatatagt aaactttgcg tttggcagta tttttagctt
     1321 tgtggctagc ttcttctttg acaaagttta ataatgatag ataaatctct tcattgctag
     1381 cctgcgtaag tgattttcct agtttagttt caacatattc tgtaaagcgt gtcatgtctt
     1441 cctcttttca ataatgtaac atttaaatat tatcttgcgc gaccataaag atctgttaaa
     1501 taaataaggt aatctttgtg atcttggttc aattcaccgt caagcatacg ccattgccaa
     1561 ttacctccaa ttgtatttgg catattcata cgactcttac catctttatc taataaatct
     1621 tggatgcaaa gaatggctgt atcacaaaca gaagcaaaga ttgttcttag catggctttt
     1681 gtaatacttt catctgcatg tttgtgaaga tagttattta caaagtgtac ttgttcttca
     1741 ctaagattgt cataccatcc gttaaccacc tcgttatcat gagtccctgt gtaagcgaca
     1801 caattacggt catagtaatg tggtaaatct atactttgac tcgttatatc aaataaacca
     1861 aattctaata tcttcatgcc aggaaagcct gttgatgcta atagctgctc tgctttttca
     1921 tcaatataac caaggttttc tgcgatgata gggagctctc ctaaggcctc acgaaccgca
     1981 gaaaataatg ctatacctgg ggctgaggcc caatggccat ttctagcagt tttatcacca
     2041 gcaggaattt cccaaaaatc tgagaagcct ttaaaatgat cgattcttag ctgatcatat
     2101 agtttgaaac tttcttgaat acgataaatc caccatgcaa aattagactt ttcatgagca
     2161 gaccaattgt aggttggatt tccccacagc tgaccatctt cactaaagcc atctgctggt
     2221 acacctgcaa taaacaatgg ctttttatca ctatctactt taaatagctc aggcatcgtc
     2281 caaacttcta cactgtccgc agaaacataa ataggcatat caccaataat ttctatccct
     2341 ttatggttag cgtaagtttt caaggctgac cattgttggt aaaagaagta ttgacagact
     2401 ttatgatagg taatcttttt tgcaagcaac tcacggtaat tattaagtga gtcttcttgg
     2461 cgtttaatga cagtttcgtc gtcccaatct tgtaaggctt tattgttaaa atattctttt
     2521 aaagccataa attcagcaaa atcagttaac cagctagaag ctgtttcaaa agcttctaat
     2581 ttacacacat tttcttgttc agcaacaaaa gctctcactg ctttttctaa aagtggacgc
     2641 ctaacttgaa aaagctgagc atagtctact gcttcaggat ttgtgccgaa tgtaatatca
     2701 cacaaatctg ctgcttctaa aagttcatca tctactagca attcaaaatc aatgaaatgt
     2761 gtgttcccag caatagctga aaatgactga taaggagaat ctccaaaact cgtcgttgtt
     2821 aaaggcagaa tttgccaata ggtttgtttc gtctctgcta gaaaatcaac aaattcaaaa
     2881 gctgattttc caaaagtgcc tatcccaaat ttcccaggca gggaactaat atgcattaag
     2941 ataccgcttg cacgtttgtt catgattttt ctccttgtga aatctattca tgatgagatt
     3001 ataacgcaag cgcttgcgta agtcaaccag tttcctaaaa aaagtaacat tccttaaaaa
     3061 tgctactttt ttttgctctt aatgactaat atcacggacg ctttctcttt gttttaaact
     3121 aaaaggaaca aaaatagtct gactaagatt ctgttcattg gaattgataa tatcaagtaa
     3181 ttgcttaaaa gacatttttc caagattttc aacatttata tcaaatgtcg ttaaataagg
     3241 gtgaattaat ttagcgtaat tagaattatt aaaagtgata attgacatat catctggcac
     3301 ttttatatca taaaaagata ataactgtac catacgaata gccagaacat caccaacaac
     3361 aaccaaggca gttgctttaa aggaagatat agtttccatc aatatttcta cgctaacagg
     3421 atccttacga tcaaataaca gcattggctt cgtttttaaa gatcgcttaa aacaaccctt
     3481 caaatagcca agataacgct cagaagttac ttctgatagt aaatcatctg ttataaataa
     3541 tatgttgtga tggccttttt gataaaggtg ttctactgct gttttagcca ttagctgatt
     3601 atcattatca atataagtgg ttttattagt gtcaccttgt ggagcaccta caatgacaaa
     3661 tggtaagtta tttgtcatca aatatttttt gacaggatca tcttgatcag aataaagaat
     3721 aataaaacca tcaactcgtt tttgaagatg catcagtttc acttgctcta tcaggtcatc
     3781 aagagatata cctgtagcaa tcgacacagt aaaatgagat tctttagctt cgttagtaat
     3841 agtcgacaaa atttccataa agaaaggttc acttaatcta tcgctagttg taataggagg
     3901 aaaaacaagt cctatattgt gtgtcaaacc actcgctaga atctgtgctg cgacatttgg
     3961 aacatatccc aaatctgcca tcgcttttct aaccttctct tttgttttca tagaaataga
     4021 tcgattatct tttaataccc tactcaccgt cgaagggtta acacctgcct tttgagcgac
     4081 atcttttatt gttaccatat taacctcgtt actatattta ccaaagataa accttatcat
     4141 gggtttgtca tgtatttcgt ttccattata caattatctt tcaaaaaatc aacttcaatc
     4201 aagattccct tgtcatttta actcaaattc tctaaactat ttttaaaaaa aacgaccttt
     4261 ttttaaaaaa aggcttgact ttttctgcaa gcggttgcat aatattaagt gtaagaaata
     4321 taccttagga ggaaatagct atgaaatcat ggcaaaaagt tatcgtcggc ggagcaagtt
     4381 tgacacttgc aagtaccttg ttagtgggat gtggatcagg ttcaaaagat aaaaaggaag
     4441 ctggcgctga ctcaaagact attaaacttt gggtaccgac tggctctaaa aaatcatatg
     4501 cagataccat tgcaaaattt gaaaaagatt ctggttacac cgttaaagtg gttgaatcag
     4561 aagatccaaa agcacaagaa aaaatcaaaa aagatgcctc aactgctgcc gatgtctttt
     4621 cacttcctca tgatcaactt ggtcaacttg tagaatctgg tactattcaa gaagttccag
     4681 aaaagtacaa taaagaaatt gctgctactt ctactgatca ggcgctcgtc ggtgcacagt
     4741 acaaagggaa aacatatgct ttcccatttg gtattgaatc acaaattctt ttctacaata
     4801 aatcaaaatt agctgctgaa gacgttactt catatgatac aattactaca aaagcaactt
     4861 ttggtggtac ctttaaacaa gctaacactt acgctactgg tccattattc atgtcagttg
     4921 gtaacacttt atttggggaa aacggtgaag atgtaaaagg tactaactgg ggcaatgaaa
     4981 aaggcgctgc tgttcttaaa tggatcgcag accaagcttc aaataaaggt tttgttagct
     5041 tagatgctaa caacgttatg tctaaatttg gtgacggttc agtagcttct tttgaatcag
     5101 gaccgtggga ctatgaagct gctcaaaaag ctatcggtaa agaaaacctt ggtgttgcta
     5161 tttatccaaa agtaactatt ggaggcgaaa cggttcaaca aaaagcattc ttgggtgtaa
     5221 aactttacgc agtcaaccaa gcaccagcta aaggtgatac aaaacgtatc gcagctagct
     5281 acaaattggc atcctacttg actaatgctg aaagccaaga aaaccaattc aagactcgta
     5341 acattgtccc agccaacaaa gaagttcaat cttcagaagc tgttcaatca aacgaacttg
     5401 ctaaaaccgt tatcactatg ggttcttctt cagactacac tgtcgtaatg ccaaaactta
     5461 gccaaatggg cacattctgg actgaaagtg ctgctattct tagtgatgcc ttcaacggta
     5521 aaatcaaaga aagcgactac cttactaagt tgcaacaatt cgacaaagat atcgctgcaa
     5581 caaaataata tgatctcatg tcatcactgc tatactaaaa caagcacaag gtaagtactt
     5641 ctaaaaagtt ctttccactt accttatgaa gagtggaact aggctccatc acattttatt
     5701 caagcttagt ctcactctta cttgtttttg ttttactagt agcttagtgc tacttaaatt
     5761 tttaactctt taaaagttta cgtaacactt agctatttca tcctaacaag agattgaatc
     5821 tgaactacca ctctagtatc ctaaaataaa ggagaatcta tatgactcag tcaaacttga
     5881 caccaaatat atctgtaata gaagctttaa aaagaggcag ttgggatatt aaactttcga
     5941 gtattatcat gggatttgcc aactttgcta acaaacagtt tatcaaagga atcttatttt
     6001 taataagtga acttatcttt ctagtagctt ttgtctccca aatcatccct gctatccgtg
     6061 gccttgtcac tcttggtact caaactcagg gaatgactac aaaaactatt gatgggatta
     6121 acatccaagt tgctgttgat ggcgataact ctatgctaat gcttattttt ggtttagcat
     6181 cactcatttt ctgcctagtc tttgcttaca tttactggtg taatctcaaa agtgctcgta
     6241 acctttatct tttcaaacaa aaaggtcaga agataccaaa cttcaaagaa gatcttgcca
     6301 cacttacaaa tggtcgcttt catatgactt taatggctat cccactgatt ggtgtgcttt
     6361 tattcaccat tcttcctttg atttacatga tctgtttggc ctttaccaat tttgatcata
     6421 atcacttgcc tccaaaatct ttatttgatt gggtgggact tgctaacttt ggaaatgttt
     6481 taagcggccg tatggcagga accttttttc ctatcttctc atggactttg atttgggctg
     6541 tttttgccac agtaactaat ttcttctttg gtattattct tgctttgtta atcaatgcca
     6601 aaggattaaa gtggaaaaaa atgtggcgaa ccatctttgt tatcaccatt gcagttccac
     6661 aattcatctc attattgatt atgcggaact tactcaatga cgaaggccca ctaaatgctc
     6721 ttctcaataa aatcggcttg attaatggct cactgccatt tttatctgat cctctttggg
     6781 caaaattctc aatcattttt gtcaacatgt ggatcggtat tccatttacc atgttgattg
     6841 cgactggtat tatcatgaac cttccaagtg aacaaattga ggctgctgaa attgatggcg
     6901 ctagcaaatt ccaagtcttc aagtctatca cattccctca aattctcttg atcatgacac
     6961 caaacttgat tcaacaattt atcggaaata tcaataactt taacgtcatt tacctcctta
     7021 ctggtggtgg tccaacaaat tcagaatact atcaggcagg aacaacagac ttgctggtca
     7081 cttggctcta taaattaacc gtcacagctg ctgactacaa tttagcttct gttatcggta
     7141 tcttaatctt tacagtttca gctatcttta gcttacttgc ttatacaagg acagcatcct
     7201 acaaggaagg agcggctaaa taatgaaaaa caaacgtcga tttcaacttg gacttgttta
     7261 tgctacttta atcatcctct caatcatttg gctcttccca atcgcttggg ttattcttac
     7321 tagcttccgt agcgaaggaa ctgcttacgt caattatttt attcctaaga cttttacatt
     7381 aaatcactac attaatcttt ttactaatga aacctttcct tttggaaaat ggtttatgaa
     7441 tactcttatt gtagccactt ttacatgtat tatttcaacc ttcataactg ttgccatagc
     7501 ctactctctt agccgaatta agtttaagtt tcgcaatggc tttttaaaac tagcccttat
     7561 tttaaatatg ttccctggtt tcatgagcat gattgccatc tactatatct taaaggcctt
     7621 aggtttgacg caaaccctaa cagcacttgt ccttgtttat tcctcaggtg cagcccttgg
     7681 tttttacatt gcaaaaggat tttttgatac cattccttat tcacttgatg aatcagctat
     7741 gattgatggc gctactcgta tggatatttt ctttaaaata acccttcctt tagcaaaacc
     7801 aatcattgtc tatactgccc ttcttgcttt tatggggcct tggattgact ttatctttgc
     7861 acaagttatc ttaggtgatg ctacaagtaa atataccgtt gctattggac tattttcaat
     7921 gcttcagcca gataccatta acaactggtt tatggccttc actgctggtt ctgtcttaat
     7981 tgccgtccca atcactctcc tcttcatgtt tatgcaaaaa tactatgttg aaggcatcac
     8041 aggtggttct gttaaataag ttacaacata ccaaaccttc taagggcaag ccctttagga
     8101 ggtttttgat aaataaggcc gtagataatc atattagt
//
LOCUS       DAUVUY010000085         7970 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.085, whole genome shotgun sequence.
ACCESSION   DAUVUY010000085 DAUVUY010000000
VERSION     DAUVUY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.085"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..701)
                     /gene="rgg2"
                     /locus_tag="VOX58_001595"
     CDS             complement(<1..701)
                     /gene="rgg2"
                     /locus_tag="VOX58_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HES5878811.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGY"
     gene            790..858
                     /gene="shp2"
                     /locus_tag="VOX58_001596"
     CDS             790..858
                     /gene="shp2"
                     /locus_tag="VOX58_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HES5878812.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            1772..2041
                     /gene="stcA"
                     /locus_tag="VOX58_001597"
     CDS             1772..2041
                     /gene="stcA"
                     /locus_tag="VOX58_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HES5878813.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQTPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQY"
     gene            complement(2389..2787)
                     /locus_tag="VOX58_001598"
     CDS             complement(2389..2787)
                     /locus_tag="VOX58_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HES5878814.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLTHHLLHSDGVIPSLNDKSA"
     gene            complement(2884..3954)
                     /locus_tag="VOX58_001599"
     CDS             complement(2884..3954)
                     /locus_tag="VOX58_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878815.1"
                     /translation="MMMLRDVKVKMSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGSSGAFPDNIK
                     VSINPRGGQATIIYGDGSTDIIPSAVLWEKGSVKEPTEADQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
     gene            complement(4205..4606)
                     /locus_tag="VOX58_001600"
     CDS             complement(4205..4606)
                     /locus_tag="VOX58_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878816.1"
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDL
                     FVFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEVSIYFYL
                     HLTVVCYTIGMILSLGRLIFPNHSIFNKLKK"
     gene            complement(5620..5820)
                     /locus_tag="VOX58_001601"
     CDS             complement(5620..5820)
                     /locus_tag="VOX58_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HES5878817.1"
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHP
                     VLAGGCVVGFTVGGAYLGYTAN"
     gene            complement(5833..6060)
                     /locus_tag="VOX58_001602"
     CDS             complement(5833..6060)
                     /locus_tag="VOX58_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HES5878818.1"
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            6379..7064
                     /locus_tag="VOX58_001603"
                     /pseudo
     CDS             6379..7064
                     /locus_tag="VOX58_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7255..7437)
                     /locus_tag="VOX58_001604"
     CDS             complement(7255..7437)
                     /locus_tag="VOX58_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HES5878819.1"
                     /translation="MDNFLELQFEELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITG
                     GAAAFVCVASGIAAYQ"
     gene            complement(7452..7685)
                     /locus_tag="VOX58_001605"
     CDS             complement(7452..7685)
                     /locus_tag="VOX58_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HES5878820.1"
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGGVGA
                     GMATLGVGGVAGAFAGAIAGGLTCVGGMLFNGK"
BASE COUNT         2646 a         1662 c         1199 g         2463 t
ORIGIN      
        1 taatacccat gcacatataa gaaaacagtc ttttcataaa aattgagctc gtctcgcagc
       61 aaaagatcaa ttttgttaat caaatagcga ctatgttcaa agcaagaatg gtcaatactg
      121 ataatgagac aattaatgga taactgagtc actaacattt tattggtttt attttgttcc
      181 gaataagcaa aagatgctac catttcctta gtcaataaaa atagggtatt gtaattcatg
      241 aagcgcgagc aattccccag aagaatgatt tcataatatc cccactgttc cactttaaat
      301 aaataatctg tgaggcgagt caattcttct tgactaggtg caattgagga gtcaatagaa
      361 aaaagaatag ctttaatcat cgttcgttca taatctttat gggcataatc cttcaacagt
      421 ttggtcaatt taaccacgtt tttctcagca taacattttc tcgcctgact taagagcgtg
      481 aaaaaatgcg tatgcgtctt ggaatgagcc gaaacaaatt cgtcaatcgt aatattcagt
      541 ttatccaaaa gattaagcaa gcgactgcag gtgatttctg attcccctcg ttcaaatctt
      601 gaaatttgtg acttggacag gtattcatct gctaaaaaag aaatgctaac ctgcttaccc
      661 ttacgtaatc gccttaaggt ttttccgagt tctttttcca tttttcccac tttcacaaca
      721 aaaaattaaa atctgctaaa tccattatat aatacaagta caaaatagaa aatcagaaag
      781 gagacagtca tgaaaaaagt taataaagct ttgcttttta cattgatcat ggatatcctg
      841 attatcgttg gtggataacc tttcgcttaa aaacaatcca cttttgggaa acccttatat
      901 ttagtttgta acaggaaaac taataaatat taaaaagagt gttagcgacc tagcggcagc
      961 tttactctta agtgcgtttg ctgccattga cagcaaggtc aaagctaatg aaacagcagg
     1021 agcataccgt tatgatgtgt ctgaacaaaa cacttactat caaactatca gctccattaa
     1081 ccagcaacga cctggtgcca ctctcgacga atgaggtgat tattatggaa cccttacttc
     1141 atttacttcc caaaatcgta ccagtactta tacacttggt agacaaagcc aacgtatcat
     1201 ggttaacagt gatattacag ttacctatac tcttaaagac aatttggtag cccttcataa
     1261 ggaatataaa acctttgtca aaaaaatatt acagataaac cgatcaccag tgactacgac
     1321 aaggctaagg ttatttataa ccatttactc aacgaataca cttacgccac aaaataactt
     1381 agcaccaatc acaaaaccgt aagcagcatc aacaatcata ctcctaaagc tctttatcgt
     1441 ggcaagtgtg gtgtttgtca agcttatgcc gtcatgttta aagatatggc agaagctgca
     1501 ggcttatcag cttggtatgt gataaaaaag gattgaatga gcaccatgct tgaaacatcg
     1561 ttaccattca tggtgttaat tcctacgttg atgctacctg gaatgattca gcatacacca
     1621 caaggtattt ccttgctgga aaagacgtga tgaatactga gtaccgttta gatagccagt
     1681 acaaccactt ggctaatact attttaacta ccagctacca aaaaggctaa actaacaaca
     1741 actacatttt tcaactagaa aggcctaaac aatgaccaaa aaacagtttt atcttatctt
     1801 agctattagc atactgtgtt ttattcttgc cgtcagccct aaaccaccaa ttcctaaaca
     1861 cactattgaa aggaaaagga ctgcgatgac cataggccac tcaaaacaaa cccctcacaa
     1921 aaaagaagcc cttaaacaca aaaaaaacaa aaataaggct ctatttcttc caagtgggga
     1981 cagcgctatc aaatctgtta ttagcatgac agttagactc ctgttagatg accaatatta
     2041 gctctcctaa aatcagtgga cactaccatt tcggtagtgt tcttttttaa gctaatctca
     2101 tcaccacgct ttcttcaagc taaaaaactg atcatgaaag agcaacccca tcaccctaac
     2161 agcccacttt gcccgcactt tcttaaaaag ttaggagaaa acgatatacc aattattttc
     2221 tgatcccagc tccctatcat taacaagatg tgtagcacgg acctacatac aggtaagcta
     2281 gagtcattct cgctacttcg gacaaaaaga ggccggtagt tccctgacct cttctttatt
     2341 tatcttgtaa cactcctctt cttagcgaga cttactagca cataattctt aagcactctt
     2401 atcatttaga ctaggaataa caccatctga atgaagcaaa tgatgcgtca gatctgccag
     2461 agtatcatct ccataagtaa tccctgtttg gtgctgtttc tgatgaaaat cagatgactc
     2521 cttaacttta gcagtcacct gctggtcact tggatgaaaa ttaatttctc tcttggcttc
     2581 agccgcaact ggctcgagga acaacttatt gacattacgg tcttcctcca aatgattaag
     2641 gatagcagca tcatcttcta acgaaagaac cttcttgggt aacaaaaagg ggttatttag
     2701 ccttggctca gtaacgactt ccttggctac tcgtgatagt ttattattgt cttcctcagg
     2761 tatcaaacgg ttaggcaatc gatgcaattc ttccggctat gaggcgctca cagctacttc
     2821 agaagaaacc tccaagccct ttcctgtttg cctgtctaac taaagcaacc ggcaaattat
     2881 taactaactt tttaacctgt cctcagcgct catttcaaca ctaggaagag ctgtcgctgg
     2941 ttctagccag ctaccaactt ctgctggtct taccttaaca tcgctggcag gcttctcctt
     3001 ctcatcttca ttaggtctaa aaggctcaaa ccgccctgtt gatttttcat ctatgacctt
     3061 tatattgtct tttactacat gagacagtgg ttcgacattt accatagctt catgctcgtt
     3121 aaggctctgg tctcgtttga atttaccagg aataccagga gtcggtgttc cgacagattg
     3181 atcggcttca gtaggctctt ttacggagcc tttttcccat aacaccgctg atggaatgat
     3241 atccgtagag ccatctccgt aaataatcgt ggcctgacca cctcttggat taatagatac
     3301 cttaatattg tctgggaaag ccccactact tcctgtggtt aaaagggcac ccgcattttt
     3361 aatgagagtt gtcattcgtt caataccagc aacgccaata tggttcaagt catctactgg
     3421 taactgatca acagatttca tggtgtattt ccaagcttgc ggttgaacaa gaaaaacagt
     3481 tcctttatac attgtgttat gcatatcatc aatgactttg aaataccttg tatatttggt
     3541 ttgtacatta gggttagcaa cgggaacagt gctttcagga actcctgaca tcgtaaaaat
     3601 ccctctagca gaagtttgaa agccctcata aacatgaccc ataccaggaa cagtctgagc
     3661 ggtcaaagac ttaccagaac catccaaagc aatgcgaata tctttcatca gatacatctg
     3721 attggtgtag gtaaacctaa ccgtgttagc ctcacctcga tacaaaacca cttgggaggg
     3781 ctgatccgaa gaaaactgag aggttgtttc ttccttatag ccaactccaa aacgtctatc
     3841 agacgaatgg actgaatctg ccgcaactgt actactcaaa ctgactagta aagtagccgc
     3901 acaaccaact aataaactgc tcattttaac tttaacatct cttaacatca tcatgttcct
     3961 ttcattattt tttatttagg aattcgtcat actatatttt ctcatattag tctaacaaaa
     4021 aacccgtaat tctatataaa tcatataaaa tgacgagctt tggcaaagat gagtatagca
     4081 ggatggaaaa cagattgttt ctgaaacttc aaaggtatct tcttgtcacg aactattctt
     4141 taattgaatc ggttacacta aactagtccc attatttaaa aacaaacttt aaatatgatt
     4201 catttcactt tttaagttta ttaaatatag agtgattagg aaaaataaga cgacctaatg
     4261 ataaaatcat tcctattgta tagcaaacaa cagtaaggtg taaataaaag taaattgata
     4321 cctctgatat actcgtcaaa catcctatta ttataataaa agcaattaag gtaggaataa
     4381 gaattgtaca cttaataaat aaccaaaatt gcttaacaac ttgataccat gtgagcactt
     4441 ctgtcgtttg tctttgtttg tagacaaaaa cgaataaatc aaggataaaa ctccaacaga
     4501 taagttgtat atttttcatt aatggtaaat cgtagaaact acctatacta aatagaatca
     4561 ctgcagaaat aataacaata cctaaaaatc ttaatatatt acgcatatct tcacctcctc
     4621 tactctatcg gtagcaaata taaccgaaca aattattatt cctgttatca gagacagtta
     4681 taactttttt aaccaatttt caatacgtat tttgctttta actctttagt ctctaaagtt
     4741 aaatatcata agtactgata ttgtataagt aatccatttt ccccttaaat gtgattgcat
     4801 gatagctcag tagaattaat cttaatttct ggtatttttt tccacaccaa gtatctataa
     4861 cgcaaatata gataaacaat aagtataaaa taaataaaga tcacaataag ggatatacct
     4921 cctagtacag cagcccaaat aggataaggg tgcctaaagt tgtaggctac actcaagatg
     4981 ataagggtaa aagggacaat gtaacctccc agcttttcaa cattttctag aaatatcatc
     5041 tttctatcat ataggttaaa aataatatct aaaattttca taccactcct ctactactta
     5101 tatatcagtg atagatagtc atttatatat ttagtgaggc cagataatat ttgtagcagc
     5161 accagctgct ccacacaaaa tataaccttg ccaagggata aatacgcctg tctgagcaca
     5221 aactgtcact ccctgcgcag cacctgccat ccctgtcaaa aagatatttg ctccgtcttt
     5281 tccgcctcca tttatgatag tcagaccatt tttttagcac ttccatattt ttgaagtctt
     5341 tttcattcta atttcactct tttgtatttt aaaaagctaa gagtgatatc taaattcatt
     5401 ggcataatca ctattttctt ttatcgataa aaacaaataa tgacgctacc aaagctacga
     5461 tagataacat gtttaccgat ttttccgaaa gggataattg attgaaaatt aaaaacacta
     5521 aatatggtat agatttatca acattttttt atgaatggca tcaacaatga ttaaaaacat
     5581 tatagacgca ttcataataa taaaaatcgt atagatcatt taatttgctg tatatcccaa
     5641 atatgctcca ccaactgtaa atcctacaac acatccacct gccaatactg gatgtggcag
     5701 tttacaaaat ttcaatccag aagctaagcc tccaagacca ccaattacag catctcctgg
     5761 agcgtttcct ccacttatac tagccatgtc attaattgtt aattccttca ttgttgtcat
     5821 cctttctttc ctctagaagc cgcctgtagc tcctgttaca ccagcccaaa gagtaatagc
     5881 tgttttagct ccaatataac cacaggctac accgcaaact ggaaaaccta tagcagctcc
     5941 agcactacct gcagcgataa ccccactgac atttgcctgc caattattct taccaccctc
     6001 aaccttggca agcttttcta tagtcatttg gtgaaatgtt tccagtttct ttatctccat
     6061 atcgaataac ttcttttcat aattcttaaa tttgtctcgt tgcaacctcg agctagtaca
     6121 gaatatcata aagaacagta tcatggtttc aaaatcttaa aaggctattt ttcatcatga
     6181 aatgtctttt tatcctctct acacttacgc tgtgacaata gttaattaat taagaagttt
     6241 aaatgatacc attcatgctt ttataaatac cttttaagat ttttgatggt agtgtaaaat
     6301 atcttgtgta aacacacaaa aaggaataaa tcttgtatag tagagttacg acacttcact
     6361 agaaagagat ttattcccat gactcagttt accacagaat tacttaactt cctagcacaa
     6421 aaacaagata ttgatgaatt ctttcgctcc tctttagaaa tagctatgaa tgacctcttg
     6481 caagttgaac tatcagcttt tcttggttat gaaccttatg aaaaagaagg ttacaacaca
     6541 ggtaatagcc gtaatgggac ttattctcgt cagtttgaaa caaagtatgg cttagtcaat
     6601 ttaatcatcc caagagatcg aaacggcgag ttttcaccag ttttattacc atcttatgct
     6661 agacgagatg accacttgga agagatggtg atcaaactct atcagactgg cgttacgaca
     6721 cgcgaaatca gtgacattat tgagcgcatg tatggtcacc acttgtttct gaatggaagc
     6781 ctcgttataa aaaggtcatg gcatcacttg aaacgacgga aaatctcctt actttctatc
     6841 aattcccaca tcacatttgg tctagcattt actctacaaa cttgattgaa tcactcaata
     6901 aagaaattaa gcgacaaaac aagaagagag tggttttccc aaatgaagaa gccttagaac
     6961 gatgtcttgt aagtattttc gaggactata atattaagtt cggagctcgt attcataaag
     7021 gattcggagc atgctttgac acacttgata gtttatttga ctaatattca aaaactctac
     7081 tcttgtgttt acacaacatt ttaatattat taatttgtaa tagtacttag taagacataa
     7141 acaaatatca caattaaaat tactttactt gcattttctc ttacctcttt atccttcaaa
     7201 aaaatttggg aaagtattag acaaattaaa ccgagtatta taactataat catatcattg
     7261 ataagctgca attccagatg ctacacatac aaatgctgcc gcacctccag ttattggacc
     7321 tcctgctgca gctataagca tcccacctaa gcaccctccg atagcagatc caatatttcc
     7381 ttttccacca gaaatattta ctaattcttc aaactgtaat tctaaaaaat tatccatttt
     7441 tcacctcaaa attattttcc attaaataac ataccaccaa cgcaagttaa tccacccgca
     7501 atagcacctg caaacgcacc tgcaactcct ccaacaccta aagtagccat acctgctcca
     7561 acaccacctg caatagcacc tgaagtaata ccaccaatta aatgattttt acaatctgta
     7621 gcactataac ctccaataat atggaacaac tcttcttttt gtatttcttc tgcaaatttt
     7681 atcataactt taaaaacctt tctaaaatgt tatattcatc ttgttgcaac tttgacaaac
     7741 taaatatagc atattattat aaaaattttt ttctatgtcg tgaatggttg tataaacatc
     7801 tcaaataaac tttttaacat attagagact gtcaataatg ttgtgtaaac acaagagtag
     7861 agtttttgaa tattagtcaa ataagctatc aagtgtgtca aaacatactc cgaatccttt
     7921 atgaatacga gctccgaact taatattata gtcttcgaaa atacttacaa
//
LOCUS       DAUVUY010000086         7718 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.086, whole genome shotgun sequence.
ACCESSION   DAUVUY010000086 DAUVUY010000000
VERSION     DAUVUY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.086"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..686
                     /locus_tag="VOX58_001606"
     CDS             <1..686
                     /locus_tag="VOX58_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HES5878821.1"
                     /translation="YANRRDVIIANRDLGPEIKAMIKRTIDRAVDAHARSNRKDAIDA
                     IVTFARTSLVPEESISAKELRGLKDDQIKEKLYQRALAIYDQQLSKLRDQEAIIEFQK
                     VLILMIVDNKWTEHIDALDQLRNAVGLRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDV
                     TRTMMKAQIHEQERERASQRATTAAPQNIQSQQSANTDDLPKVERNEACPCGSGKKFK
                     NCHGRKSFS"
     gene            802..1158
                     /locus_tag="VOX58_001607"
     CDS             802..1158
                     /locus_tag="VOX58_001607"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HES5878822.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWAGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            1155..2255
                     /locus_tag="VOX58_001608"
     CDS             1155..2255
                     /locus_tag="VOX58_001608"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HES5878823.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKAVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            2342..3853
                     /locus_tag="VOX58_001609"
     CDS             2342..3853
                     /locus_tag="VOX58_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HES5878824.1"
                     /translation="MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSNNKA
                     EAENQVDDKSTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEARTPIQSLSPY
                     VSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKSTGIAYD
                     STRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAFDLDPTQ
                     ASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFTDTTGSG
                     KRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLGYQASSY
                     AWGQCTWYVYNRAKELGYQFDPFMGNGGDWKYKAGYALSKTPKVGYAISFAPGQAGAD
                     GTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSKN"
     gene            4343..>7718
                     /gene="shr"
                     /locus_tag="VOX58_001610"
     CDS             4343..>7718
                     /gene="shr"
                     /locus_tag="VOX58_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HES5878825.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKTVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVTITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLKKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVEKGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDW"
BASE COUNT         2613 a         1318 c         1558 g         2229 t
ORIGIN      
        1 tctatgctaa ccgtcgtgat gtcataattg ctaatcgaga tcttggccca gaaattaaag
       61 ctatgatcaa gcgtactatc gaccgtgctg tagatgctca tgcacgtagc aatcgtaaag
      121 atgctataga tgctattgta acttttgcta ggacctcgct ggtaccagaa gagtctatta
      181 gtgctaaaga gttacgtggt ttaaaagatg atcaaatcaa agagaagctt tatcaaagag
      241 cgcttgctat ctacgaccag caactatcaa aacttcgtga tcaagaagct attattgaat
      301 tccaaaaagt actcattttg atgattgtgg ataacaaatg gactgaacac attgatgcct
      361 tagatcaact tcgtaatgct gttggacttc gggggtatgc tcaaaataac cctgtggtgg
      421 aataccaagc agaaggtttc aagatgttcc aagatatgat tggtgctatc gagtttgatg
      481 taacacgtac catgatgaaa gcacaaattc acgaacaaga acgtgaacgt gcaagtcaac
      541 gtgccacaac agcagcacct caaaacatcc aatcgcagca gtcagctaac accgatgatt
      601 tacccaaagt ggagcgtaac gaagcttgtc catgtggttc aggcaaaaaa ttcaaaaatt
      661 gtcacggtcg taaatccttc tcataagaac gagacacaac aggacaaagt taggtatata
      721 gaaagaagct gagaataatt ctcagcttct cactgtttaa atcttctaac attaagatga
      781 aataaggttt ggtggaaagt aatgattgtt ggacatggaa tagacttaca agagatttca
      841 gctattgaaa aagtttatca aagaaatcct cgctttgctc aaaaaatttt aactgagcag
      901 gaattggcta tttttgagag ttttccttat aaacgtcgac tcagctattt ggcgggtcgt
      961 tgggcaggca aagaggcttt tgctaaggct atagggacag gtattgggcg gttaaccttt
     1021 caagatattg agattttaaa tgatgttaga ggatgtccca ttctgacaaa atctccgttt
     1081 aaaggaaata gttttattag catctcacat agtggcaatt atgtacaggc tagtgttatt
     1141 ttggaggata aaaaatgatt tcaagtttcc atcgcccaac agttgcaagg gtcaacttac
     1201 aagctattaa ggagaatgtt gccagtgttc aaaagcatat tccactaggg gtaaaaacgt
     1261 atgcagttgt caaggctgat gcttatggtc atggtgctgt ccaggtgtca aaagcactcc
     1321 tacctcaagt ggatgggtac tgtgtgtcaa atcttgatga ggctttgcaa ttacgtcaag
     1381 caggcattga taaagagatt ttaattcttg gagtcctcct gccaaatgaa ttagagttag
     1441 cagttgctaa tgctattact gttacaatcg ctagtttaga ctggatagct ttagctagac
     1501 tggagaaaaa agaatgtcaa ggcttaaaag ttcatgtaaa agttgattct ggtatggggc
     1561 ggatcgggct tcgttcttca aaagcagtca atttattgat tgatagtcta aaagagttgg
     1621 gtgctgatgt agaaggtatt ttcactcatt ttgccacagc tgatgaggca gatgatacta
     1681 aatttaacca gcagttacag ttttttaaaa agctgatagc tggacttgag gataagcctc
     1741 gtttagtaca tgctagtaat tcagccacaa gtatctggca tagtgatacc atttttaatg
     1801 ctgttcgttt aggaattgtt agttatggtt tgaatccaag tggttctgat ctaagcttac
     1861 catttccact gcaagaggct ttatctctag aatctagctt agtgcatgtc aagatgattt
     1921 cagctggtga tacagtcggt tatggagcta cttatactgc caaaaagtct gaatatgtag
     1981 ggaccgtccc aatcggttat gcagatggct ggaccaggaa catgcaaggc ttttcggtgt
     2041 tagttgatgg acaattctgt gaaattatag ggcgtgtatc gatggatcaa ctgaccatac
     2101 gacttcccaa agcatatcct ttaggaacaa aagtcacttt gattggcagc aatcagcaaa
     2161 aaaatatttc tacaacagat atcgcaaatt accgtaatac aatcaattat gaagttctat
     2221 gccttttaag tgaccgtatt cctcggatat attaagaaaa tcacgtaaaa ataattgcat
     2281 tgtgcttatc tttaatgtta taataaagtg aggacattag aaagaagtga ctaattaata
     2341 tatgaataaa aacaaactat taagagttgc catgctacta agtctcttag ccccgacagc
     2401 agaaagcatg acagtgttgg ctcaagatgt aatgcttgag acgcataaag caactacaaa
     2461 tgaaaccagt gattcttctt caaaagagga aaataataaa aatgcagcac ctacaacatc
     2521 agataaaact gaccaaggtc cccttgatgc ttctgcagaa acaaactcta atagtcttgt
     2581 taacgcggat gataaaaaaa gaagcgattc tagtcagtct gctgtaggct cttcgaacaa
     2641 caaggcagaa gcagaaaacc aggtagatga taaatcaact gatcattcga aaccaactga
     2701 ccagccaaaa ccgactgatc agcccaaacc atcaccatct aaagttgata cggcacctgc
     2761 ttcttcattg tcgagacagc tgccagaggc aagaactcct attcagtcgt tgtcccctta
     2821 cgtatcagat ttagatttga gtgagataga tatcccttct gtcaacacat acgcggcata
     2881 tgtagagcat tggagtggta aaaatgccta tacccatcat cttttatctc gccgttatgg
     2941 tattaaagct gaccagattg atagttactt aaaatcaaca ggcattgcct atgacagcac
     3001 acgtattaat ggtgagaagc tattgcaatg ggaaaagaaa agtgggctgg atgttcgagc
     3061 tatcgtagct attgcgatgt ctgagagttc tttaggaact caagggattg cgactttgct
     3121 tggagctaat atgtttggct atgcagcttt tgatctagat ccgactcaag caagtaagtt
     3181 taatgatgat agtgctattg tcaaaatgac acaagatacc attattaaaa acaaaaatag
     3241 caattttgca cttcaagatt taaaagcggc taagttttca cgaggtcaat taaactttgc
     3301 aagtgacggg ggtgtttatt ttactgatac tactggtagt ggtaaacgtc gcgcacaaat
     3361 tatggaagac ctggataagt ggattgatga ccatggtggc acaccagcca ttccagccga
     3421 attgaaagtg cagtcatcag ctagttttgc atctgtgcca gcaggttata agctctctaa
     3481 gagttatgat gtcttgggtt atcaagcttc gagttatgct tggggacaat gcacttggta
     3541 tgtgtataat cgcgccaaag aattgggtta ccaatttgat ccttttatgg gaaatggtgg
     3601 agattggaag tataaagcag ggtatgccct gtcaaagact ccaaaagtag gttatgctat
     3661 ttcatttgca ccagggcaag caggcgctga tggcacttat ggccacgtat caattgtaga
     3721 agatgttaga aaagatgggt ctattcttat ttcagagtct aactgtatcg gcttaggtaa
     3781 gatttcttat cgtaccttta cagctcagca ggctgaacag ctaacatatg ttattggcaa
     3841 gagtaaaaac taataactaa tgacttttct taaggcttaa ttttcttagc gggagttagg
     3901 ccttttaaac tgttggtgta acacgataaa gtttcaagta aataaatgat tttgagacaa
     3961 tagcacgatt agggctattg tcttttttta ttgtgatgaa tatgcagtag tgacatctca
     4021 tgttgtctcc taacacaatt atggagctta taacgtgtta catgttttgt gaatgaagta
     4081 atgattgaat cagccctagt aataactatc ctaaggctag ctttatctca gtaatattgt
     4141 tgtagaggaa ttcttttatc agagatatca tcattatcaa tccaggctta ttgctagtgt
     4201 cttggctcac atagtggagc tttttaacag acaattggac cattggaaac aatcttgaca
     4261 aaataatcac aaagtgatag attaagttca gttaataaaa aagttagctt attcataaaa
     4321 atttttagaa agttgattga aaatgaaaaa aatcagcaaa tgtgcgtttg tggcaatatc
     4381 tgcccttgtt ctcattcagg ctactcaaac tgtaaaatca caagagcctt tagttcagtc
     4441 acaactcgtg acaacagtag ctttaactca ggataatcga cttttagttg aagagatagg
     4501 cccttacgct agtcaatcag ctggaaaaga gtattataaa catattgaaa aggttattgt
     4561 tgataatgat gtctatgaaa aaagcctgga aggcgagcga acctttgata ttaactacca
     4621 agggattaag atcaatgcta accttattaa agacggtaag catgaattga ctattgttaa
     4681 taaaaaagat ggtgatatcc taattacctt tattaaaaag ggcgataaag tgacctttat
     4741 ttcagctcaa aaattaggaa caacagatca tcaggattca ttaaaaaaag atgtgctcag
     4801 tgataaaaca gtgccacaaa accaaggcac acaaaaagtt gttaaatctg ggaaaaatac
     4861 tgctaacttg tcattaataa caaaattgag tcaagaagat ggtgcaattt tatttccaga
     4921 aattgatcgt tattctgata acaaacagat aaaagcattg actcagcaaa tcacaaaggt
     4981 tacagtcaat ggtacagttt ataaagatct tatttcagat tctgtaaaag atactaatgg
     5041 ctgggtctcg aatatgacag ggcttcatct tggaacaaaa gctttcaaag atggagaaaa
     5101 tacaatcgtg atatcctcaa aaggatttga agacgttacc attaccgtta ccaagaaaga
     5161 tggtcaaatc cattttgtat ctgccaaaca aaaacaacat gtgactgctg aagacagaca
     5221 atcaacaaag ttggatgtca ccactttgaa aaaagctatc aaagaagcgg atgcgattat
     5281 tgctaaagaa agcaacaaag acgcggtcaa agatctggct gagaaacttc aagtcatcaa
     5341 ggattcttac aaagaaatca aagatagtaa gctactcgcc gatactcatc gactgttaaa
     5401 agataccatc gagtcttatc aagcaggtga ggtttctatt aacaatctca cagaaggaac
     5461 ctatacgcta aactttaaag ctaataaaga aaactcagaa gagtcctcca tgcttcaagg
     5521 tgcttttgat aaaagagcca aattagtggt taaagcagat ggtacaatgg aaatttccat
     5581 gcttaatact gctttaggac aatttttgat tgacttttct attgaaagca aagggaccta
     5641 cccagcagca gtgcgtaaac aagttggcca aaaagatatc aatggtagct atattcgaag
     5701 cgaatttacc atgcctattg atgatttgga taaattacac aaaggtgctg ttttggtatc
     5761 agccatggga ggtcaagaaa gtgatttaaa ccactatgac aaatacacca aacttgacat
     5821 gacctttagt aagaccgtta ccaaaggctg gagtggttat caggtagaaa ctgatgataa
     5881 agaaaaaggg gttgggactg aacgtcttga aaaagtttta gttaaacttg gcaaagattt
     5941 agacggcgat ggtaaattat caaaaacgga attagaacag attcgaggcg agttgcgtct
     6001 agaccattac gagttaactg atatttcttt attgaaacat gctaaaaata ttacagaact
     6061 acatctggat ggaaaccaaa ttacggaaat tccaaaagag ttatttagtc aaatgaagca
     6121 acttcgattt cttaacttaa gaagtaatca tttaacttat ctagacaaag atacatttaa
     6181 aagcaacgct caattaagag aactctactt atcaagtaac tttattcact ctcttgaagg
     6241 aggactattc cagtcgcttc atcacctgga gcaacttgat ctttccaaga atcgtattgg
     6301 ccgactttgt gataacccat ttgaaggatt gtctcgcctg acttcattag gtttcgcaga
     6361 aaatagtctt gaggagatac ctgaaaaagc gctagagcct ctaacatcac ttaactttat
     6421 cgacttatct caaaataatt tagcactact gccaaaaaca atagaaaaat tgcgcgcctt
     6481 aagcactatt gtggcaagta gaaatcatat tactcgtatt gataatattt catttaaaaa
     6541 tcttcctaaa ttatctgtac tcgatttatc aactaatgaa atttcaaatc ttccaaatgg
     6601 tatatttaaa cagaataacc aattaacaaa acttgatttt ttcaataact tgcttactca
     6661 ggttgaagaa tcagtatttc cagatgttga aacgcttaat ttagatgtga agttcaatca
     6721 gataaaaagt gtgagtccaa aagtaagagc tcttatcgga caacacaaac tgactccaca
     6781 aaaacatatt gcaaaacttg aagcttcctt agatggcgaa aaaataaaat atcatcaagc
     6841 tttcagtctt ttagatttgt attattggga gcaaaaaaca aattctgcca ttgataaaga
     6901 actagtgtct attgaagaat atcaacaatt gttacaagaa aaaggttcag atactgtctc
     6961 tttacttaat gatatgcaag tcgattggag tattgtgatt cagttgcaaa aaaaagcttc
     7021 caacggacag tatgtgacgg ttgacgaaaa gcttctctca aatgatccga aagatgactt
     7081 aacgggagag ttttctttaa aagatccagg tacatatcgg attcgcaaag ctttaataac
     7141 aaagaaattt gctactcaaa aagaacatat ctatttgaca tctaatgata tccttgtggc
     7201 gaaaggacca cattcacatc aaaaagattt agttgagaaa ggccttagag cattaaatca
     7261 aaaacaattg cgtgatggta tttactattt aaatgccagc atgttaaaaa ctgacttagc
     7321 atctgagtcc atgtcaaaca aggctattaa tcatcgagtg actttggtag ttaaaaaagg
     7381 tgtttcctat ttagaagttg agtttagagg gataaaggtt ggtaaaatgt taggctacct
     7441 tggtgaattg agctatttcg tagatggtta ccaaagagat ttagctggta aaccagttgg
     7501 tcgaacaaaa aaggcagagg ttgtgtctta tttcacggat gtaactggcc taccattggc
     7561 agatcgttat ggaaaaaact atccaaaagt gctgcgtatg aaattgattg aacaagcgaa
     7621 gaaagacgga cttgtgccat tacaggtctt tgtgcctatc atggatgcca tttcaaaagg
     7681 gtctggcctt caaaccgttt ttatgcgttt agactggg
//
LOCUS       DAUVUY010000087         7413 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.087, whole genome shotgun sequence.
ACCESSION   DAUVUY010000087 DAUVUY010000000
VERSION     DAUVUY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.087"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(126..1019)
                     /locus_tag="VOX58_001611"
     CDS             complement(126..1019)
                     /locus_tag="VOX58_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878826.1"
                     /translation="MTTMLDFPFHHEFIEFLGRWTHILITLLKRHHGQADTAIPRYLL
                     VTIREKTGIVKSTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEPLNLIALCPDCFLTYQADSRKKIVTALKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHKATL
                     QDIYFCQTIVSYFIQKCEVLE"
     gene            1035..1211
                     /locus_tag="VOX58_001612"
     CDS             1035..1211
                     /locus_tag="VOX58_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HES5878827.1"
                     /translation="MGQNVVMRQAPGGNIKPDKEKSVEKIDGIVGLILGLDRCIRRQG
                     DEASVYDERGILSF"
     gene            1266..1568
                     /locus_tag="VOX58_001613"
     CDS             1266..1568
                     /locus_tag="VOX58_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HES5878828.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLIFSFEDAKTGNRKDVREFLSQLKENA"
     gene            1568..1873
                     /locus_tag="VOX58_001614"
     CDS             1568..1873
                     /locus_tag="VOX58_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HES5878829.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQEM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            1892..2164
                     /locus_tag="VOX58_001615"
                     /pseudo
     CDS             1892..2164
                     /locus_tag="VOX58_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2269..3042
                     /locus_tag="VOX58_001616"
     CDS             2269..3042
                     /locus_tag="VOX58_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HES5878830.1"
                     /translation="MGLLDLIGRKRARDKPQNSYEGQDFFYLFGRTTSGENVDEFKGI
                     KVEFIQLSNKNIFDVLEKDLSKKLRFEDISIEAILGKYLCNNDIEIIKYLYEKDKINM
                     ELLISLISKISNKLVKQEFIKVLVLYEYVKNFLPADCIYFSLSNLYGTGHYSSMNYKI
                     FIRTKSGDIFDIADGGRIDDMVSKFNKVNVLGVCMGIGTTVLSQEIEYEIEDRIMILV
                     EKIDVKIYKNCLELANKLSGYQSFFNMNYIRDIIILLFD"
     gene            complement(3484..4610)
                     /locus_tag="VOX58_001617"
     CDS             complement(join(3484..4376,4376..4610))
                     /locus_tag="VOX58_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HES5878831.1"
                     /translation="MSTVKRYDEEFKQSLVNLYQTGKTQSELCKDYGVSASALAKWIK
                     QYSQVKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHDTTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKI
                     EGTLLFHSDQGSQFKARKLRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KKYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEEN"
     gene            4914..5711
                     /locus_tag="VOX58_001618"
     CDS             4914..5711
                     /locus_tag="VOX58_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HES5878832.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYHGEILFADMMTKEQYTEVAKKVLANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            5715..6539
                     /locus_tag="VOX58_001619"
     CDS             5715..6539
                     /locus_tag="VOX58_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HES5878833.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            6539..>7413
                     /locus_tag="VOX58_001620"
     CDS             6539..>7413
                     /locus_tag="VOX58_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HES5878834.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQNK
                     IEVVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHPSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATHDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEK"
BASE COUNT         2348 a         1191 c         1575 g         2299 t
ORIGIN      
        1 ctcttctgag atagcgtgaa agatatcttt gacgtttgag tttccacttt tgatttcatt
       61 tagcactcta ggcaaataag caagggtgct tttttgatag gagtaaagtt tatttggtaa
      121 ttgcattatt caagtacctc gcatttttgg ataaagtaag agacaatcgt ctgacagaag
      181 tagatgtcct gtagtgtagc tttatggagt ttttccgaaa tagtattgaa aatagccaga
      241 ttattatgct tggctgcttt taacttttta tacatcgatc gcgtttgata ttggatttcc
      301 tcgtaatcaa tttttccttg tttgtccaaa tttacgatga ttttttgaat ggttaagtag
      361 ttatcaataa ctgggttttt aaccaattga taaagcgtac gattgttctc aggtgatatc
      421 ttatcagtca attgcttcga gtcataggag atatcatatt catcacattt tagtttattg
      481 agactagtca aaactgaaac aattccactg tctaacttca tatcagaaat cgcttgttgg
      541 ctgttatgtg cagagactag aatcttttta acatttttta gagctgttac aatttttttg
      601 cgactatctg cttgataggt taaaaaacaa tctgggcaga gggctatcag attcagtggt
      661 tcagcgtcct tatctttttc aatggctgaa acctcgtaat tttctgctgc taagttgcca
      721 ctggttagaa tgagtggtct atcacagcct gggcatgcac aacagttatt agattctgtt
      781 aacaagaact gcccgtattg ttttttgagc tgattcgact gggtagtgaa cgcagtattt
      841 tgcacagtgg ttgattttac aatccctgtc ttctctctta tggttactag aagatacctt
      901 ggaattgcgg tgtcggcttg accatggtgc cttttgagca gagttataag gatatgagtc
      961 caccgtccaa ggaactctat aaactcatga tggaagggaa aatctaacat ggtggtcatc
     1021 cagtccttaa atggatggga caaaacgtgg tgatgcgaca agcccctgga ggcaacatca
     1081 agccagataa ggaaaaatct gttgagaaga ttgacggtat tgtaggtctt atcctggggc
     1141 ttgaccgttg cattcgccgc caaggtgatg aagctagcgt ctatgatgag cgagggatat
     1201 tgagttttta gttgagtcaa ctctaaaaac gtgatacaat gtcgttataa aaaaggagga
     1261 taaccatggc taaaacggga actttgaatt tacgagttga tgattcagta aaaagtgcgg
     1321 cagatgatat tttgaaacgc ttaggtattc ctatgtcaac tgcgattgat atgtttttga
     1381 atcagattat tttgactggg ggtatcccat ttgatgtttc tctacctgaa gcgcctcaac
     1441 gagttaatgt tgattacatg agtcaggaga agttttatga taagcttatc ttcagctttg
     1501 aagatgcgaa aactggcaac cgcaaggatg tcagagaatt tctatctcaa ttgaaggaaa
     1561 atgcttaatg aaagaatatc aggtcaccat gtcggatgat gctaaagcag atttgctgag
     1621 catctatcac tatgttcgtg atgaactctg tgcaccacag gcagcggata atcttcttga
     1681 gaggttatcc caagaaatgt tatcactatc catttttcct gaacgttgtg ccatcattga
     1741 ggatctgatt ggaaaaggct ataccttcag acaactgatt gtcaaaaaat atcgcattgt
     1801 ataccatgtt ctggaagatg aagttataat tgttgcggtc gtctatggat cacgtcatat
     1861 gaataattgg taaagtaagg gagagtgatg tatggaactc gtaaagacga tacaaattgg
     1921 tgatgacatc tatcttccga tacctgaccc atttggcatt caagagaatt aggagtttaa
     1981 cctttataaa tcaactgatg gaactttggt attaagtcca tctgatagca aactatcagc
     2041 ggatcaccaa agtttaagca gtgatgagca gatgacaaca gttgttgaac aagcaaggtt
     2101 agatgagatt gcgacttcag tattatcacg acatcttgac gcatttaagg aattgacaga
     2161 atgagaatac taggttttgt aagaaagact gttgattact ttgaaagaaa tataccgaaa
     2221 gcacttcaaa ggaggtgctt tttttgtacc ccaaaataag gagggcttat gggactactt
     2281 gatttaattg gaagaaaaag ggctagagat aagcctcaaa acagttatga aggtcaggac
     2341 tttttctacc tctttggtcg cacgactagt ggcgagaatg tggatgagtt taaaggaatt
     2401 aaagtagaat ttattcaact gtctaacaaa aatatatttg atgttttgga gaaagattta
     2461 tctaaaaagt taagatttga agatatttca attgaagcaa tattgggtaa atatttgtgt
     2521 aataatgaca tagaaatcat aaaatatctt tatgaaaaag acaaaataaa tatggaactc
     2581 ttaattagtc ttatttcaaa aataagtaac aaactggtga aacaggaatt tataaaagtt
     2641 ttagtcttgt acgagtatgt aaaaaacttt ttaccagcag attgcattta cttttcatta
     2701 tctaatttat atggaacagg acattattca agtatgaact ataaaatctt catacgaact
     2761 aagtcaggtg acatatttga tattgctgat ggtggtagaa ttgatgatat ggtttctaaa
     2821 tttaataaag taaacgtatt aggagtttgc atgggaatag gaaccactgt tttatcacaa
     2881 gagattgagt atgaaattga agatagaata atgatattag ttgaaaaaat agatgtgaaa
     2941 atctataaga attgtcttga attagcaaat aaattaagtg ggtatcaaag tttttttaac
     3001 atgaattata ttcgagacat cattatatta ttgttcgatt agatggaagt atggaataca
     3061 gattttcatc tgtagcttta aaaaataaaa ttaaaagcat acttgatagt tgatgtatac
     3121 tgtcatttag aataataatc acatattgtt gtcaacgaaa cccaccatag tctataatga
     3181 aagcctagta gggatgagct atataccaac tatctatcaa tccagacgtc tctactaaaa
     3241 aatactccgt cttctatgaa actagaaacg gagtatttct gtttgtctat accactaaat
     3301 gaacagttat atagaaaggt gattggatgt ttggaataaa catcatgaaa aaaatttgag
     3361 accttcttat atcctaaact agaacgaatg tctagtgaaa taaaaaaaac gacaccatta
     3421 ggatgtattt tggactaatg tcaaataagt agacagaaaa ccgtgttatt ttattacgtt
     3481 aaactaattt tcttctttct gattaggggt tagtcctaga ttagccgtat gtgggttgta
     3541 attgttataa aaattctcaa tgtattcaaa gcagtctaat tgaacctgtt tgatattttg
     3601 atactttttt cggttgattt gtctatgctt taaatacttg aaaaatgctt cagttacggc
     3661 attatcataa ggatatccag gtttagaaaa agaatgcatg atagtgttgt catcaattat
     3721 ttttctcaat ttcctggcct taaattgtga cccttggtcg gaatgaaaga gaagtgttcc
     3781 ttcaatcttt cttttattaa gagctaattc tagagtgtca catgctaact ttgcatccat
     3841 atgatgactc agtttccaag caatacattt tctagaatag agatcaagta ttgcgcagag
     3901 atagacatat ttcttatatc caatagaaat ataggtgaag tcagtagacc atacttgatt
     3961 tggtttgtca ggattaaatt tctggttgag taagttttga ggatacgttt tttgagtcct
     4021 tttaagagct gtttttggct taacggtagc cattttaggg agcgccatat tcttcataag
     4081 acggtaaatt cttccttcag agatttttgt gtcgtagtct ctttgaagaa tgaccttgat
     4141 agacttcaca ccaagtcttt tgtttgcttt ggtataaatc tcaagtattt gttttctata
     4201 aatttgatta tccaattctc tttttgaggg cttatgtttt agaaatttat agtaagtgga
     4261 gcgattgaca cgtaaaacac gacataaggt tgttgtgtcg tgttcaaagc gtaaccgata
     4321 gacagcgagg agtcttactt taagttttgc atgaatatgg cacttgcttt ttttaggata
     4381 aggttctcct cttcaagctg cgcattacgt ttttgtagct cttgtatttg cttggcagta
     4441 agtacagaat tatcttcgag tttgacttga gaatactgtt tgatccattt tgcaagcgca
     4501 gaagcggata ccccatagtc tttacagagt tcagactgag tttttccagt ttgataaagg
     4561 ttgacaagag attgtttgaa ttcttcatcg tagcgtttaa ctgttgacat aggttgtcct
     4621 ttcgtttttg tgtcttttaa aacagattgt aacacacaaa gttttgtcta cttttatagt
     4681 atacatccag tggtcattta taaaaataaa aaagtagcag ttcacttcaa aaacggtcag
     4741 gttatcgaaa tataatctgg ctgtttttta cgaatggaat gaaagctgct ttttatgata
     4801 taataagaaa aattgaacag aatgcagaaa tgatggggct tatttaaatc gttaaaaagt
     4861 gcacacccta aatggcgttt gcacccccct agagtaaaaa aaggagacat tgtatgatta
     4921 aactaatagc aacagatatg gatggtacct tcctagctga agatggtact tataatcaag
     4981 agcaactagc tgcgctcttg ccaaagttag ccgaaaaagg cattttattt gcggtttcga
     5041 gtggccgctc gctcttagcc attgatcagc tctttgagcc ctttttggat cagattgctg
     5101 ttattgcaga aaatggttcc gtggttcaat accatggtga gattctattt gcagatatga
     5161 tgaccaaaga gcagtatacc gaagttgcaa aaaaggtttt agccaatcct cactatgtag
     5221 aaacaggtat ggttttttct gggcaaaagg cagcctatat tttaaaaggc gcatcagaag
     5281 agtacattca aaaaacaaaa cattactatg ctaatgttaa ggttattaat ggttttgaag
     5341 atatggagaa tgacgccatt tttaaagttt ctaccaactt cacaggccat accgtcttag
     5401 aaggaagcga ttggttgaat caagccctcc cttatgccac tgcagtgacc accggctttg
     5461 attccattga tattatctta aaagaggtga ataagggctt tggtatggag catctttgtc
     5521 aagccttggg cattaagaaa gcagaaacga ttgcttttgg ggataatttt aatgattacc
     5581 agatgttgga gtttgctggt agggctattg ctacggaaaa tgcaagacct gaaatcaaag
     5641 tgatttcaga ccaagtcatt ggccactgta atgacggagc agtactaacg tatttaaaag
     5701 gactagtgta actcatgaat attagaattt tggcgcttga tttagacggt accctctaca
     5761 acactgaaaa aattgtcaca gatgccaata aaaaagcctt agcagcggct agagaaaaag
     5821 gggttaaggt ggttattacc acaggtcgcc ctctaaaggc catcggtaac ttgctagaag
     5881 agttggactt gcttgaccac gatgactaca gtattacctt taatgggggt ttggttcagc
     5941 gaaatacagg ggaggttttg gataagagct ctctcagttt tgaccaggtt tgccagattc
     6001 agcaagcttt agaggcggtt ggcttaccaa cagatattat cagtggtggg gatgtgtata
     6061 gcattccaag taaggacggt aggcattcgc agtaccatct agctaaccca ctgttgacct
     6121 ttattgaggt gacatctgta gcggaactac caaaagacat tacctataac aaaattgtga
     6181 cagtcaccga tccagacttt ttggatcagc agattataaa gctatcccca agcttatttg
     6241 aggattttga agcctttaaa tcacgagata ttatttttga aatcatgcca aaaggcattg
     6301 ataaagcttt tggattaaac ttgctttgcc agcatcttgg ccttgacgct agacatgtca
     6361 tggctatggg agacgaagct aacgactttg cgatgttaga atgggcagga cttggagtag
     6421 ctatggctaa tggtgtttca ggagctaaag ccgttgccga tgccgtgacc accttaacca
     6481 atgatgagtc tggagtcgca gaagctgtga agacatttat tttagaagag gagagttaat
     6541 gggattattt gatcgtttat ttgggaaaaa agaaaccccg aaggtagctg aggaaaagtt
     6601 agaagaaaat cttttgacag aaacaactca gaaagaggag ctttctgaaa aagccaatga
     6661 gcaaaacaag attgaggtgg ttcaacaaga ggacgtttct tcagagggcg caggttcggt
     6721 ggaaaatggt cccgaagcag cttcagtaaa cgcacaggtt gaggaagaaa caggtgacaa
     6781 tagtaatcat ccatcagaag ataccaatga gtttgctgcc gacaaaacag accttaaggt
     6841 aagtgaactt agtcaatcta cggcatcaga accaaaagac cttgttgacc agccagttgt
     6901 ggaacagttt ccaacaaaac aagctcaagc tgatgcctca aatgattcgg cgaatgaaga
     6961 agcagtagac acttcaaaag agcaatccag tagccaacag gttatggaag actactatcg
     7021 tcgtaaggct gctcttgaaa aaagccttca agaaaaagca gcggcaacgg ttccagtgat
     7081 gccagaagaa gttccacaag aaaatcaggc ctctacctca gcagaagctt ctcaaaacaa
     7141 ggcaacccac gataccattc ctgagacaga tcaagaaaaa tacaagcgta gcttgaaaaa
     7201 aacgcggact ggcttctcag ctcgtctgaa cagtttcttt gccaatttca ggcgagtgga
     7261 tgaagaattc tttgaagact tggaagaaat gctgatctta tcagatgtcg gtgtacatgt
     7321 ggcgacaact ttgacagagg agttgcgtta tgaagccaaa ctggaaaatg cgaaaaaacc
     7381 agatgccttg aaacgtgtga ttgtggaaaa att
//
LOCUS       DAUVUY010000088         6965 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.088, whole genome shotgun sequence.
ACCESSION   DAUVUY010000088 DAUVUY010000000
VERSION     DAUVUY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.088"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            255..482
                     /locus_tag="VOX58_001621"
     CDS             255..482
                     /locus_tag="VOX58_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation repressor RelB"
                     /protein_id="HES5878835.1"
                     /translation="MAMVSLRLNSAEEELFRSYATHTGKTLSELFKTALAQQIEDQLD
                     YETGIQALKRFEENPITYSIDDLIEELENDL"
     gene            472..741
                     /locus_tag="VOX58_001622"
     CDS             472..741
                     /locus_tag="VOX58_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HES5878836.1"
                     /translation="MTYKLVVSDEVKKQLKKMDKHVGLMLAKDMKKRLDGLNNPRQFG
                     KALTGQYKGLWRYRVGNYRVICDIVDNEMIILALEVGHRKEIYKR"
     gene            complement(1115..1648)
                     /locus_tag="VOX58_001623"
     CDS             complement(1115..1648)
                     /locus_tag="VOX58_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HES5878837.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFSTEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
     gene            1783..4056
                     /locus_tag="VOX58_001624"
     CDS             1783..4056
                     /locus_tag="VOX58_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HES5878838.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEQLSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     GLQTLSQGNSLAQLDQLKAAVSQIASQSNQALPGASSALSELSMGLSKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTVIGSGVIKLSEGANALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(4173..5246)
                     /locus_tag="VOX58_001625"
     CDS             complement(4173..5246)
                     /locus_tag="VOX58_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HES5878839.1"
                     /translation="MKNISRKCFMTSVVCIVLGAILLGAGYATGGLQDIKHQTAPKKV
                     IQTFDHVTALDIDTNFSTITVETGPVKKPTVTYYTHSKFIDPLEIKIENETLHISEKP
                     RDVIITGGIELIGFGLNKSDRNHNYRTTTITLPKGTSLKELKGSSMPQATISDLTVQD
                     LEFSGGLTLLNTRIKKGKVFGTFNATDSLLANLELKADYSLSSLTNSSLENGTISLGH
                     GQLTTKNTNLKSVTIQSLSPGGVETEETSLENVTFTVTSEQENEETNDNGAIFTAHAL
                     TLKGTNTISGGNVAVDITLTDPKTTAYKTKTEDGKILLGSQLTPAKIGKESTSDVISY
                     VAENKAATGNLTVNLNKGDITIK"
     gene            complement(5243..5830)
                     /locus_tag="VOX58_001626"
     CDS             complement(5243..5830)
                     /locus_tag="VOX58_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HES5878840.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEALEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGLAGILVAGVTLFESLTLIGSSIPAQAMGIGSALLCFG
                     GGLLIWIIATALLRFSGRAFVAFIKWISHKKGAQA"
     gene            complement(5817..6143)
                     /locus_tag="VOX58_001627"
     CDS             complement(5817..6143)
                     /locus_tag="VOX58_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HES5878841.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
     gene            6350..>6965
                     /locus_tag="VOX58_001628"
     CDS             6350..>6965
                     /locus_tag="VOX58_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HES5878842.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWKSIQPNEEVDKVFIVPLRQLLFTDPVYYRLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWGMTAQFTKCLTDILNDESIAKKNPS"
BASE COUNT         2178 a         1241 c         1364 g         2182 t
ORIGIN      
        1 atagcagaac ttaacagaag taaaagctgg tattatgaga ctaagagacg tgctttactt
       61 gaatttgtgg aattttacag agacggtgtg ctaaaaaaat aatgttcgtt tatgataaca
      121 gagcaataga tataaatgtt aataatacag tgttagaaga gcttaacaag gtatatcaat
      181 acgggtaaat aattgactga ttcctcctta tgttatacaa tgtattacag ataaacaaac
      241 agaggtggaa gaatatggca atggtatcat taagactgaa cagcgcagag gaagaacttt
      301 tcagaagcta cgctacacac acaggcaaaa cattatctga attattcaag acagcactag
      361 cacaacaaat tgaagatcaa ttggactatg aaacaggtat tcaagcttta aaacgctttg
      421 aagaaaatcc cataacttat tctatcgatg atttaattga ggagttggaa aatgacttat
      481 aagctagtgg tgagtgatga agtcaagaag caactcaaaa agatggataa acatgtaggg
      541 cttatgttag ccaaagacat gaagaaacga cttgacgggt taaataatcc tagacaattt
      601 ggaaaagccc tcacaggtca atataaagga ctttggcgct atcgtgttgg taattatcgg
      661 gtgatttgtg acattgtgga taatgaaatg attatccttg ctttggaagt tggacacagg
      721 aaagagatat acaagcgcta gcaaatacaa gacatggtac gaggtataat aaaaagcgca
      781 gctaatgacc acgctatttc ttgcttactg aactcattat tttaattcct ttttagttac
      841 cctttagatt tctgatagtg tatctagttt acactgtttt tagagaaata agagtgataa
      901 ctcacttggt ttacagctac tttacactac taaatatgag tagtataaca aaatgcttta
      961 attttaagat taaacttaca aaaagcctat ttaataggct ttttttgtat ctcataacaa
     1021 atttatcctg atcccttcat tttactatgg cttctaaaaa agttatcact aaggctgatg
     1081 acttgtaaaa tgatactttc gtaagggtta ttatttaggt actcgtaagc attttgagaa
     1141 cgaattgtgt catttcttga ggtgattctt ttttaccttt agcaatccaa ctttgacaga
     1201 caccaaaaaa agcatgggct aagtagatac tttggtattc tttttccgtt tgagataact
     1261 cttcggtact gaacttgtcc tgtaaatcag tagtgatgag taggcgaact ttattgatga
     1321 taaaggcctg tatttcttta gtaccgttag cagacaaaag ggaagagaga agctgttctc
     1381 tttggagaaa ttcaaatact tcaaggaagg cttgttcttt attttgatat tttttctcaa
     1441 aaatatattc taatttgtga aaaaaggttt gctgataata gtcaatcatt tcatatttat
     1501 ctttatagtg tgtgtaaaaa ctagagcgac taattccagc tcttttagat aatttaactg
     1561 tggtaatatc gtcaaaactt tcggttttta gtaacataac cattgctttt aaaattgctt
     1621 gcttcgtatt ttctttacgg ttgaccatct accatctctc cttttgaaca cttttagacg
     1681 taatgtccaa aagtgaattt ttttctcttg taaatttaaa tttaaattgt ataatatatt
     1741 ttatcataat ttttgaacag tatgtccatt ttaggagaaa atatgttaga agaattgaaa
     1801 acacttatta aaaatccaaa attaatgatt acaatgattg gtgtggccct agtgcctgcc
     1861 ttatataatt tatcctttct aggctcaatg tgggatcctt atggtcgggt caatgacctt
     1921 cccattgctg ttgttaatca tgataagcct gcaaagagag ctgataagtc attgacaatt
     1981 gggaatgata tggtggacaa gatgtctaaa agtaaagatt tagagtatca ttttgtctca
     2041 gctaagcaag ctcaagaggg acttaaagaa ggtgattatt atatggttat tactttaccc
     2101 gaagatcttt ctcaacgggc agcaacctta ttaaatcccg aaccccaaaa attaactatc
     2161 cgttaccaaa cgagtaaagg acatggaatg gtcgctgcta agatggggga aacagcgatg
     2221 gctaagctga aagagtctgt ttcgcaaaac attacgaaga cttatacctc agcagttttt
     2281 agcagtatga cagacctcca atcaggatta aaagaagcct cagctggaag tcaagcactt
     2341 gcttcaggag cgaagaccgc tcaggcgggg agtcaaacac tttcgacgaa cttagcagcc
     2401 ttaacgggtg ctagccaaca gtttcaacaa ggtactggtc gattgacatc aggtttgact
     2461 acctatacag atggtgtcaa ccaagtcaag aatgggttag gaacattatc aacggacatc
     2521 cccaattatc tgaacggggt ttctaggtta tcccagggag cttctcagct taatcagggt
     2581 ctttcacagt tgacacaagc aacaacactt tctgacgaga aagctaaggg aattcaatcc
     2641 ttaattgtag gactaccagt cctaaatcaa ggcattcagc aactaaatac agagctatca
     2701 acattgcaac cccctaacct taatgctgat gagttaggta atagcttagg agctatcgct
     2761 caagctgcca aacaagttat tgctgaagag actgccgctc agaatgaaca actatcggct
     2821 ctccaagcca ctagtgttta tcaatcatta actgctgaac aacaaggaga gttagctgcg
     2881 gccctcagtc aatctgataa aagtcaaacc gtatctgcag cccaaactat tttaagttct
     2941 gttcaaacac tgtcaacagg tttacaaact ctgtcccaag gaaatagttt agctcaatta
     3001 gaccagctta aagcagctgt ttcacagatt gcgagtcagt ctaaccaggc tttacctgga
     3061 gcgagttctg cattatctga gttgtcaatg gggttatcaa aggtaaatgg tagcttaaat
     3121 caacaagttc taccaggaag taatcaattg acaacaggat tagcacaatt aaacaggtat
     3181 aatactgtca ttggttctgg ggtaataaaa ctctcagaag gtgccaatgc cttgtcatcc
     3241 aagtccggag aattactaga tggtagccat caattatcag agggtgctac taaactagct
     3301 gatagtagtt ctcaattgag tcagggtggt catcaattaa cgagcggatt gactgaatta
     3361 tcaacaggat tatcaacctt aaatggttcc ttagccaaag cctctcagca gttatcgctt
     3421 gtttctgtga ctgataaaaa tgctaaagct gtcgcaaaac ctcttgtgtt aaatgagaaa
     3481 gacaaagatg gtgttaagac gaatgggatc gggatggcac cttatatgat tgctgtttct
     3541 ctaatggttg tggccctttc aaccaacgtc atttttgcta attctttatc tggtcgtccg
     3601 gtcaaagata aatgggattg ggctaaacaa aaatttgtta ttaatggttt tatttcgact
     3661 atgggatcca ttgttctcta cttagctatt caattattag ggtttgaagc ccgttatggt
     3721 atggaaacct taggatttat tatgctaagt ggttggacgt ttatggctct tgtcacagct
     3781 ttggtcggtt gggatgatcg atatggctct tttgcttctt tggtcatgtt attacttcag
     3841 gttggctctt caggtggctc ttaccccatt gagttaagtg gagcattctt ccaaaagtta
     3901 catcctttct taccaatgac ttatgtggta tctggtttac gacaaaccat ttcattatca
     3961 ggtcatattg gagtagaagt gaaagtctta actggtttct tactggcatt tatggtatta
     4021 gcactactca tttatcgtcc caagaaaaca gtctaaataa aataacctca aaaaaaaaca
     4081 gcatctaagg cagttacact agatgctgtt ttttgtctta ttttgacaat aacttgacaa
     4141 tttctgtcaa gttttttatt gtcttgttaa gattatttga tagtaatgtc tcccttattg
     4201 agattaaccg ttaaatttcc agtagctgct ttattctcag ccacataaga aataacatct
     4261 gaagttgatt ccttaccaat cttagctggt gtcagctgtg atccaagtaa gattttacca
     4321 tcttcggttt ttgtcttata ggcggtcgtc tttggatcgg tcaaagttat gtcaacagca
     4381 acattaccac cactaatagt attagtgcct ttaagggtaa gagcatgagc agtgaatata
     4441 gcaccattat cattggtttc ctcgttttct tgctcactag taacagtgaa agtcacattt
     4501 tcaaggcttg tttcttcagt ttcaacccca ccaggagata gagattggat ggtgacagat
     4561 ttaagattag tattttttgt ggttaattgt ccatgcccta agctaatcgt cccattttct
     4621 aaactagaat tcgtaaggct tgacaacgaa tagtcggctt tcaactcaag attggctaac
     4681 aagctatcag tagcgttaaa tgttccaaaa accttccctt tctttattct agtatttaga
     4741 agtgtcagac caccactgaa ctcaaggtct tgaaccgtta aatccgatat agtggcttga
     4801 ggcatgcttg agcctttgag ctctttaaga ctggtacctt ttggaagcgt tatggtagtg
     4861 gttcggtagt tgtggttgcg gtcactttta tttaatccaa acccgatcag ttcaatacct
     4921 ccggtgatga tgacatctct gggtttttca gaaatatgaa gtgtctcgtt ctctatttta
     4981 atttctaaag gatcaataaa tttagaatgt gtgtaatagg taacggttgg ttttttgaca
     5041 ggccctgttt ctactgtaat agttgaaaaa ttggtatcaa tatcaagagc tgttacgtga
     5101 tcaaatgttt ggataacctt tttaggggct gtttggtgtt taatgtcttg aagcccgcca
     5161 gttgcataac cagctcctaa aagaatggct cctaaaacaa tacacacgac actggtcata
     5221 aaacatttgc gtgagatatt tttcatgctt gagctccttt cttgtgacta atccatttaa
     5281 taaaggcaac gaaggcacgt cctgaaaaac gcaggagtgc agtggcaata atccaaatga
     5341 gaagaccacc accaaaacac aacaaggcgc tgccgattcc catagcttgt gcaggaatag
     5401 atgagccaat caaggtcaag ctttcaaaca gggtcactcc tgcgacaaga atacctgcaa
     5461 gcccaaaaat gtaggcggca aagatagctg tgagcccgag cattaggcag gcaataataa
     5521 agagaatcaa cgccagcaat acgggtaaag caatcggagc agcaaagagg gacaaaatgg
     5581 ttaatccaat aattgttgcc cgatttttgg gagttttctc gggagttgcc aagtgttttc
     5641 ctagaacatt tgaaatgatt tcactggcag cttccttagg atttcctaaa tcgtcgataa
     5701 cctggctttc tttgtcagga ccagcttcat caaaatattc aatataatat tccaacgctt
     5761 catggtagtc ttctctaggt agtttgcgca agtatttatc tagttctgct agatactcag
     5821 tccttgtcat gtcttaagct cccttcaata atcccgtcta aggcaaattt atagctctgc
     5881 cattcttttt ttagaaaaat cagctgtgct ctccccgaag aagtgacagc ataatattta
     5941 cgctttcgtc cctgatgttc ttgactataa gttgtcaaaa aacctgcctt ctccaatttt
     6001 ttaagaatag gatagagggt ggattctttg atattagcca ctagtttaat ggtttgacta
     6061 atatcgtaac cgtaagaatc gtttttttcg acaatggcta aaatcagaaa ttcaatcaag
     6121 gtagccgata ctggaaaata cattttaggc tcctttcata tataaaaatt ttatatataa
     6181 ttattttaca tatttagtat aagcttagtt ttttgatatg tcaatatttt atatataaaa
     6241 aaataacata tatatcaaaa atcactaact aggagtaata aattgaccaa aaagctagga
     6301 ttggttatga tgaagataag attaacggaa agagaaatta gggaaggcta tgaaggactt
     6361 attaaaacag tatcaggcaa aaccactagg agaggagaag cggtacgccg tatttttacc
     6421 tcttattctt gtcaatgatg actggcatgt cttatatgaa gtcagaagtc agcatatttc
     6481 acaaccgggg gaagtttctt tcccaggggg acaagtagaa aatcaggaga cacttcaaga
     6541 agctgctatc cgagaaacag ttgaggaatt gactgttgat gcgtcccaga tccagctatg
     6601 gggtgaaatt gattatttag tacagtcttc tagaactatt cattgtttcg ttggtcaatt
     6661 agtcgttgat gactggaaaa gcatacagcc taacgaagaa gttgacaagg tctttatagt
     6721 acctttacgt cagctacttt tcactgatcc tgtctattat cgtttagaag ccactcctat
     6781 tgagacaacg gattttcctt ttgaccgtat tcgtaatggg aaatactacc aatttagtca
     6841 agagtaccgt agtattccgt tttatgaaaa tctagaagaa accatatggg gaatgactgc
     6901 ccagtttact aagtgtttaa ccgatattct aaatgatgaa tcaattgcaa agaaaaaccc
     6961 ctcag
//
LOCUS       DAUVUY010000089         6828 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.089, whole genome shotgun sequence.
ACCESSION   DAUVUY010000089 DAUVUY010000000
VERSION     DAUVUY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.089"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..393
                     /locus_tag="VOX58_001629"
     CDS             <1..393
                     /locus_tag="VOX58_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HES5878843.1"
                     /translation="ASFGLCLAGWAVNSKGTSLAHVLRSPKISMANVLSKPKIMLPML
                     CSAAVLGVIGAIFNIQGTPASAGFGISGLIGPINALNLAKGGWSPVNILLIIIIFVGA
                     PIVLNMIFNYLFIKVLKVIDPMDYKLNI"
     gene            408..1400
                     /locus_tag="VOX58_001630"
     CDS             408..1400
                     /locus_tag="VOX58_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HES5878844.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESPLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLREMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWGLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDAAKAVLDYKESVEEAIKDADFVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARGALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            1505..2170
                     /locus_tag="VOX58_001631"
     CDS             1505..2170
                     /locus_tag="VOX58_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HES5878845.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLAFRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     TDD"
     gene            2163..2879
                     /locus_tag="VOX58_001632"
     CDS             2163..2879
                     /locus_tag="VOX58_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HES5878846.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILLITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGVCMFMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            3025..4371
                     /gene="trmFO"
                     /locus_tag="VOX58_001633"
     CDS             3025..4371
                     /gene="trmFO"
                     /locus_tag="VOX58_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HES5878847.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAKSVTAELENHPLIEVIRDEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHDALTNAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRNNPNLFFAGQMTGVEGYVESAASGLVAGTNAARLFKREEALVFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            4524..5930
                     /locus_tag="VOX58_001634"
     CDS             4524..5930
                     /locus_tag="VOX58_001634"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HES5878848.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLAAVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQVRQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPV
                     NEDLKRSQIGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFL
                     TTKYQTDDVIKVTAFIKA"
     gene            5973..6287
                     /locus_tag="VOX58_001635"
     CDS             5973..6287
                     /locus_tag="VOX58_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878849.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKTL
                     SQQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVER
                     KS"
     gene            6287..6637
                     /locus_tag="VOX58_001636"
     CDS             6287..6637
                     /locus_tag="VOX58_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HES5878850.1"
                     /translation="MKRYEVTVNGQVYEVSLLELADGETVQVSRPAAPATEKEMNANA
                     AGGGLQVKAPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            6647..>6828
                     /locus_tag="VOX58_001637"
     CDS             6647..>6828
                     /locus_tag="VOX58_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HES5878851.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAG"
BASE COUNT         2051 a         1319 c         1415 g         2043 t
ORIGIN      
        1 gcaagctttg gactttgctt agcaggttgg gctgttaatt caaaaggaac ttcacttgct
       61 cacgtcttga gatcaccgaa aatttcaatg gccaacgttt tatcaaaacc aaaaatcatg
      121 ttgccaatgt tatgttctgc agctgtactt ggtgttattg gtgctatctt taatattcaa
      181 ggaacacctg ctagtgcagg ctttggaatt agtggtttga tcggacctat caatgccctt
      241 aatttagcta aaggcggatg gtctccagta aatattctac ttatcattat catttttgta
      301 ggggcaccca ttgtgcttaa tatgatcttt aactacctct ttattaaggt gcttaaagtg
      361 attgatccaa tggattacaa gcttaatatc taagaaagga acttttgatg aaattaaaat
      421 tatataatgt tagaggcgaa gaagcagtct tagcaaaaaa atgggctgat gacaatggga
      481 ttgagatttc cttgacagag tctcccttaa cccctgaaac ggttaaagaa gctgaaggct
      541 ttgacgggat tgccaatgcc caaattggtc ctttagatga tgcaatctat cctctcttaa
      601 gggaaatggg gattaaacaa atcgctcagc gcagtgctgg tgtcgatatg tataaccttg
      661 atttggccac cgaaaatgac attattatca caaatgttcc tagctattct ccagaatcca
      721 ttgcagaatt tacggttact attgccctta atttaattcg tcatgtggag ttgattcgtg
      781 aaaatgttaa aaaacaaaat ttcacttggg gactccctat ccgcggtcgt gttttaggtg
      841 atatgacagt tgcaattatc ggaactggac gtattggtct agctactgct aaaatcttta
      901 aaggttttgg ctgcaaggtc gttggatatg atatttacca aagcgatgct gccaaagctg
      961 tcttggacta taaggaatct gtagaagaag cgattaaaga tgctgatttt gtttccttac
     1021 acatgccacc aactgcagag aacacacacc ttttcaatgc tgatttattt aaatcattca
     1081 aaaaaggggc tattttgatg aatatggcgc gtggtgccct tattgagacg caagatttac
     1141 tggatgcttt ggatgcaggc ttattgagcg gagctggtat tgacacttac gaatttgaag
     1201 gaccttatat tcctaagaat tttgaaggtc aagaaattac agattcacta tttaaagctt
     1261 tgattaacca tcctaaggtt atttatacgc ctcatgcagc ttattatact gatgaagccg
     1321 ttaaaaacct agttgaaggt gcccttaatg ctacagtaga gattatcaaa actgggacaa
     1381 cgacaactcg tgttaactaa caactgattt gacgtccatt ataaaggaat aattccaaaa
     1441 attcctttat aatggacttt tttgcattag tccgttataa tagactgtaa gaaagggggc
     1501 tcttatgaca tatatagcgc ttattggaga tattatccag tccaaacaat tgacggatcg
     1561 ttccaaagtt caaaaaactt tagcagctta tttagacgac ctcaacaaaa cctttgctcc
     1621 ttatattatt tctaaattat ccctaacgtt gggagatgaa tttcaaggac tttttcaggt
     1681 ggatactcct attttccatc tgattgattt gattaatcat cacatggata tccctattcg
     1741 atttggagta ggagtaggtt ctattctaac agacatcaat cctgatatta gtattggggc
     1801 tgatggacct gcttattggc atgctcgtga ggctattcgt tatattcatc aaaaaaatga
     1861 ttatggtaat actacattag cttttcggac tggtcatcac aaccaggatg atgtattaaa
     1921 cagtttacta gcagctggag atgctattaa agccaactgg cgagctagtc aatgggaaat
     1981 ttttgacacg cttttagatt tgggcattta tgaggaatac tttgatcaac aacgactagg
     2041 aaagcaatta tctcttagct ctagcgcttt gtcaaaacgt ctaaaaagta gtcatgttaa
     2101 aatttatctg agaacacggc agagtgcgct aaactgtctc aaacaaatca aggaggaaac
     2161 tgatgattaa tggtgtctct cactacctag ctcaaacccc tacacttacc ctgtttctca
     2221 tttgtcatgt gttatcagac taccagttac aaagtcagca agtagctgat ttaaaagaaa
     2281 aacatttgac ttatttaggt taccacttga ttggagttag cattccccta atttgtttaa
     2341 ccctcattat cccacaagca tggttgatga gcctattggt tatgataagt cacgcgctta
     2401 ttgactggct aaagccaaag atggctaact ctctaaaatg gaaaagagag tggatttttc
     2461 tgctggatca atgcttacat atcgctatta gttcatttgc tgggctaagg ttagcagggg
     2521 tgaccttgcc taattggctt ccgatatcta ttttgatgac cgttttattt atcctactca
     2581 ttaccaaacc aactaatatt gtctttaaac tctttttcag caaataccag cctgaccaag
     2641 gagaaaaaat ggacacgatc ataggagcgg gggctaccat aggaattcta gagagaattg
     2701 ttattggggt gtgtatgttc atggggcaat ttgcttcaat aggtctggtc tttaccgcta
     2761 aatctattgc tcgctataat aaaatttcgg aaagtccagc ttttgcagaa tactacctaa
     2821 ttggttcttt atttagtatt ctcagtgttt ttatcgcagc atggatttgt ttcttctagt
     2881 tgaaaagggt tcccgtagaa agaggctaca catagcctct tttgcttttt tcccctttct
     2941 gttataataa gtcaggtaac tacattagaa agatttgtgg gtctgtcatc taagagatat
     3001 cattcccgaa gaggtattag tgacttgtct caatcaactg caacttatat taatgttatt
     3061 ggagctgggc tagctggttc tgaagctgcc tatcagattg ctaagcgcgg tatccccgtt
     3121 aaattgtatg aaatgcgtgg tgtcaaagca acaccgcaac ataaaaccac taattttgcc
     3181 gaattggtct gttccaactc atttcgtggt gatagcttaa ccaatgcagt cggtcttctc
     3241 aaagaagaaa tgcgtcgctt ggattccatt attatgcgta atggtgaagc taatcgtgta
     3301 cctgctggag gagcaatggc tgttgaccgt gaggggtatg caaagagtgt cactgcagag
     3361 ttggaaaatc atcctctcat tgaggtcatt cgtgatgaaa tcacagaaat ccctgacgat
     3421 gccattacgg ttatcgcgac gggtccattg acttcagatg ccttggcaga aaaaattcac
     3481 gcgctaaatg gtggcgacgg attctatttt tacgatgcag cagcgcctat cattgataaa
     3541 tctaccattg atatgagcaa ggtttacctt aaatctcgct acgataaagg cgaagctgct
     3601 tacctcaact gccctatgac caaggaagaa ttcatggctt tccatgatgc actaacaaac
     3661 gcagaagaag caccgctgaa tgcctttgaa aaagaaaagt attttgaagg ctgtatgccg
     3721 attgaagtta tggctaaacg tggcattaaa accatgcttt atggacctat gaaacccgtt
     3781 ggattggaat atccagatga ctatacaggt cctcgcgatg gagaatttaa aacgccatat
     3841 gccgtcgtgc aattgcgtca agataatgca gctggaagcc tttataatat cgttggtttc
     3901 caaacgcacc tcaaatgggg tgagcaaaag cgtgttttcc aaatgattcc agggcttgaa
     3961 aatgctgagt ttgtccgcta cggcgtcatg caccgcaatt cctatatgga ttcaccaaat
     4021 cttttaaccg aaaccttcca atctcgtaac aatccaaatc ttttcttcgc aggtcagatg
     4081 actggagttg aaggttatgt cgaatcagct gcttcaggtt tagtagcagg aaccaatgct
     4141 gctcgtttgt tcaaaagaga agaagcgctt gtttttcctc agacaacagc tattggtagc
     4201 ttgcctcatt atgtgactca tgccgacagt aagcatttcc aaccaatgaa cgtcaacttt
     4261 ggcattatca aagagttaga aggcccacgc attcgtgaca aaaaagaacg ttatgaagct
     4321 attgctagtc gtgctttggc agatttagac acctgcttag cgtcgcttta acactaaaaa
     4381 agagttcctt ttcagttgag ttgctgaaga ggactttttt ccttgctgat ttgagcgcta
     4441 gctatgcaaa agaatgcaca aaaaatacat gaatagacta tggtcactag aagcaatatt
     4501 atataatgaa tagtactcat tagttgattc agtttggagg ttttagcatg cagcagcaag
     4561 tcgcaataac agaaacagtt ttacgggacg gtcatcaaag tttgatggca acgcgtttat
     4621 ctattgagga tatgcttcct gtccttacca tcttagacaa gattggttat tattccctag
     4681 agtgttgggg aggagcaacc tttgatgcct gtattcgttt tttgaacgaa gacccttggg
     4741 aaagactgcg cacgttaaaa aaaggcttac ctaatacccg tttacaaatg ttattaagag
     4801 gccaaaacct tttaggctac cgtcattatg cagatgacat cgttgacaaa ttcattagtt
     4861 tatcagctca aaatgggatt gatgttttcc gcatttttga tgccctaaat gacccacgaa
     4921 atatccagca agccttaaga gctgtgaaaa agacgggtaa agaggctcaa ctgtgtattg
     4981 cctatacaac tagcccagtg cacacgctta actattacct ttctttagtg aaagaattgg
     5041 ttgagatggg agctgattct atctgcatca aagatatggc tggtattcta actcccaaag
     5101 cggctaaaga gttagtatct ggtatcaaag ccatgactaa cttacctctt attgtgcata
     5161 cccatgcaac tagtggcatt tctcagatga cctatctagc agcagtagaa gctggtgctg
     5221 atagaattga tactgctttg tcacctttta gcgaaggcac ttcacaacct gctacagaat
     5281 ccatgtattt agccttaaaa gaagcaggtt atgatatcac attagatgaa acgctactgg
     5341 aacaggctgc taatcacctt cgacaagtcc gtcaaaagta cttagcagat ggcattctgg
     5401 atcccagtct tctctttcca gacccaagga cactacaata ccaagttcct ggaggcatgt
     5461 tatctaatat gctatctcag ctgaaacaag ccaatgccga atcaaaatta gaagaagttt
     5521 tagcagaagt tcctcgggta agaaaagact tgggttatcc tcccttagta acaccgctta
     5581 gccaaatggt cgggactcaa gctgctatga atgtgatttt aggaaaacct taccagatgg
     5641 tgtctaagga aattaaacaa tacttggcag gcgactatgg caaaacacca gcaccagtca
     5701 acgaagactt aaaacgaagt caaattggct cagctcctgt caccacaaat cgtcctgcag
     5761 atcaactaag tcctgaattc gaggttttaa aagcagaagt agcagattta gcccaaacgg
     5821 acgaagatgt gctgacttat gccctcttcc caagcgtagc aaaaccgttt ttaacaacca
     5881 aataccaaac tgatgacgtc attaaagtga ctgcctttat caaagcataa taggctatct
     5941 taatcagata gaaacaaaaa tatggagatc aaatgaccta ttcctttatt gatatcttac
     6001 aagttacctt agtatccttg ttcttagtgt taacctgttt aacagggatt atgggattaa
     6061 tgatgctaag tggtaaatta ttgtcacaag aaaaaaaaac tctctctcaa caagcttcca
     6121 gcattcctca agagcaagaa gctgacaagt tgagtggaga agctctcgaa actcttttag
     6181 ccaaagacga attggctaga attgctgtta tgactagtct tgcttatgct agccaagaag
     6241 agtcaggaaa acggtttgaa gttattgccg tggaaaggaa atcctaatga aaagatatga
     6301 agtgactgtc aatggacagg tttatgaagt gagtttactt gaactagctg atggagaaac
     6361 tgttcaagtt agccggccag cagcacctgc tactgagaaa gaaatgaatg ccaacgctgc
     6421 tggcggtggc ctacaggtaa aggctccaat gtctggaaca gtcttgtcta tctttgctac
     6481 agaaggaaaa gctgttaaaa aaggagaagc tgttctggtc ttagaggcca tgaaaatgga
     6541 gaatgaaatt ctagcacctg cagatggtct ggtttcaaaa attcatgttg tggctaacca
     6601 aacggtcgag tctgaacaag ttttgatttc attctaggag gaaaccatgc tagacgtact
     6661 caatcaaatg gtccaatcgt caggattggc tcatctaact gttaataatc ttatcatgat
     6721 ttgcttggcc tgtttctttt tataccttgg tatcaaaaag gaatatgagc cttaccttat
     6781 ggtaccaatc gcctttggga ttttactcgt taatttgcct atggcggg
//
LOCUS       DAUVUY010000090         6517 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.090, whole genome shotgun sequence.
ACCESSION   DAUVUY010000090 DAUVUY010000000
VERSION     DAUVUY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.090"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(1..945)
                     /locus_tag="VOX58_001638"
                     /pseudo
     CDS             complement(1..945)
                     /locus_tag="VOX58_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1225..2766)
                     /locus_tag="VOX58_001639"
     CDS             complement(1225..2766)
                     /locus_tag="VOX58_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5878852.1"
                     /translation="MLHLHLETKLQDKLSLLNILLDVSEVSIDQLCQETELKKQRVYN
                     LLFEMIKDLEDTLTLTICDDTVSIPYKTYQLKMPYFKKLYQTSIFLKMLCFLIEPGEL
                     SLHDFIKREYISQATAYRIRTNCRKYLKKVGLNVRQNHIVGPEYRIRFLIALLHYQFG
                     MTIYDFDKTSMNKVVSLIINSNQAITLNDASKAPYEFSYFAILISLIWKRRHDNLGIP
                     QTDAFKHLKKLSIYRDIKMTSQEIIGKWYHPDLTDEDLDYIFLCFCTTNNSFHKDKWT
                     PKKVKELFELVMTKTNGKTLKASLRPLLGNNILNSLPFKRILVSFSRLFISNLQVLLP
                     DIHLFHYLRRQQKRNKSFYNTLKTIVEEWMSAEGIVGKLPSYHLLLFTIQLEELLKTY
                     LPPIPVYLLTNNTAALDLMTNALSIYFPPAIATVMPVNVEIIPFKDIVKEKQSVIIAD
                     RQYLNLIQHLYQNQGHLFLYFLFSFRDVSEAYIHKAFLDFRQKRYDEFIVTLLDTYHK
                     NLSSP"
     gene            4122..>6517
                     /locus_tag="VOX58_001640"
     CDS             4122..>6517
                     /locus_tag="VOX58_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HES5878853.1"
                     /translation="MLKKIEVKEGQKNPISITLSEDQAKSLKNKEKLKVSIKQKQSKK
                     TSKDFFFEVGIDPKVKAKQQEKLLELDKVKKQIEDSINGDAWLPEKSEGEKPVQNTNK
                     ELQLQELNKKYQMAKEAIESATTLDDVETQFDKYTKVGDKDKYPDSLRNQYTRGDKDK
                     EIEKAKKSLGDLSDKVNGKIEEDKWLSDEVKKKQQQELEARKQKVNDSLKGSDSLKSL
                     RETVEKASSKNQKKPESFEDVYVPGNEETEKTKVRDILQKTYQKTEQNIETDPWLSPE
                     QKKAQKENAKARLDAGLKAVETTESLDKLKEVESDFLDKEKNPDSIPNQHKAGTPEVA
                     RKTFLDNFEKEAKKEIESIDKDDTLTANAKQVAKDKVAQQLQEATAKVEKAQSFDDLK
                     NIQDKFNSELPHTPGKPLKDQQSDAIAELEKKQQEIEKAIEGDKTLPRDEKEKQIADS
                     KERLKSDTQKVKDAKNADAIKKAFEEGKVDIPQAHIPGDLNKDKEKLLAELKQKADDT
                     EKAIDSDKTLTAKEKEEQKAKTKEELQKATEAVGAIDNREELDKKVPELKKAIEDTHV
                     KGNLEGIKNKAIEDLKKAHTETVAKINGDDTLDKATKEAQVKEADKALAAGKEAITKA
                     DDADKVSTAVTEHTPKIKAAHKTGDLKKAQVDANTALDKAAEKEREEINNDITLTAKD
                     KEQQLKEVETALTKAKDNVKAAKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVA
                     KLEEAAKATKDKISADPTLTSKEKEEQSKAVNAELKKAIEAVNAADTADKVDDALGKG
                     V"
BASE COUNT         2383 a         1052 c         1313 g         1769 t
ORIGIN      
        1 gcaatcgtat gtggcttggt tgaagctgtt tgcgattgcc tttttcgttt ctcatagaag
       61 tctgcgatat gacaaggatt ggtatgacta gcaaaagcaa tgtcatggat acacttgaaa
      121 agaatctttg aagcataagg attcccacgt ttggtaatgt gctcttgagc tagatagttg
      181 ccagactcat aatgtctaaa gtcaatccca atgaaagcat taatttgatt agcagattga
      241 aaacgacgaa tagcaccgag ttcgccaata atacttgttg cagttgtttc tgcgatactt
      301 ggaatagagc gtagaatctt gtcttctggt aaaggttgag ccaacgccac catcttatca
      361 aaaatagctt gacgacgttc agaaagtcta agtaattctt tggcatagta gcgaacctct
      421 tctatcatcg gagaggtttt cgtaacagta aaataagact gcttggctaa tgagacaagt
      481 ttttccacta agtagatccc acgcttgtca gagatacgtt tagaggtaga ttgacggatg
      541 atagctgaca atgctttgtc agctagctcc agtacaaagc tattacatgg aaaagccgtt
      601 accacattcc agtattactc accagttggt gcaaataaga tgtgttccag ttcaggaaaa
      661 gtgacttgta agatcttgtg aaggcgattc ttaactctcg aacaatatta tcctcagtca
      721 atttttgata gaagcgactc aagtcacgta gcagctgata aacttcttcc tgaacgtaag
      781 ttggttttct aactagaata aattgagact gagccaattt ttcagtgtct aacttatccg
      841 ctttacgcac ccgtagacta tctagttttc taaaggattg agttgtgtat aagcgtagcc
      901 atttttctca aggaaggact gaagccgacg agaatagact cctgttgtga tggtctccct
      961 tttgcctttt aaacaatagt atagccgttt ttcaagatgt tattaatgat tttttccttt
     1021 attctagaag aataagtctg atactgtttc ttcttaataa tctcaatgcc ctaacagtcc
     1081 atcagtcgaa cggtatttct caaattactg cagttgactc ttgcgttcag ttccctcacc
     1141 ttgtggatgc cgtttagaac aatattagct tgctgaatta gcttgctgag ccttttggta
     1201 atgtgaacaa agcatcttta tcactcaagg agaagacagg ttcttgtggt aagtgtccaa
     1261 caaggtcacg ataaactcat cgtaccgttt ttggcggaag tctagaaatg ccttatgaat
     1321 gtaagcctca gaaacgtctc taaaactaaa caagaaatac aagaacaaat gcccttgatt
     1381 ctggtacaaa tgctgtatca ggttcaggta ttgcctatct gcgataatga cactctgctt
     1441 ttccttaaca atatctttga aaggaatgat ttcgacattg acaggcataa ccgttgcaat
     1501 agctggcggg aagtaaatcg agagtgcatt ggtcatcaaa tccagcgcag cagtgttatt
     1561 ggtcaaaaga tagacaggta tcggtggcaa atatgttttt aggagttcct ctaactgtat
     1621 cgtgaagagc agtaaatgat aggacggcag tttccccacg atgccttctg cgctcatcca
     1681 ttcctcaacg atggttttta gagtattgta gaagctcttg tttcgttttt gctgtcttct
     1741 gaggtaatgg aacaagtgta tatcaggtaa caaaacttga agattactga taaatagacg
     1801 tgagaaggaa accaaaatcc gtttgaaagg aagggagttt aggatattat tgcctagtag
     1861 tggacgcagg gatgctttca aggtttttcc atttgttttt gtcatcacca actcaaacaa
     1921 ttctttcact tttttaggtg tccatttatc cttatggaaa gaattattgg tggtgcaaaa
     1981 acataagaaa atataatcca aatcctcatc agttaaatca ggatggtacc attttccgat
     2041 gatttcctga ctggtcattt tgatatctcg atagatagac agttttttca agtgtttgaa
     2101 tgcatctgtc tgagggatcc ccaagttatc atgtcgccgt ttccatataa gggatatcag
     2161 aatggcaaag tatgaaaatt cataaggggc tttactggca tcattaagtg tgatggcttg
     2221 attggagttt ataatcagcg aaaccacttt attcattgat gttttatcaa aatcataaat
     2281 ggtcatacca aactgatagt gcaacaaggc gatcagaaag cggatacggt attctggtcc
     2341 aacaatatgg ttttgccgaa cgtttaaacc tacctttttc aagtatttcc gacaattggt
     2401 cctgatacgg taagcggtgg cttgggaaat atactctcgt ttaatgaagt cgtgtaggct
     2461 cagctctcct ggttcaatta aaaaacataa catctttaag aatatcgagg tttgatagag
     2521 tttcttaaaa taaggcattt taagttggta ggtcttatag gggatagaaa cagtgtcgtc
     2581 acaaatggtt aaggtgaggg tgtcttctaa atccttaatc atttcaaaga gtaagttgta
     2641 gacacgttgt tttttgagtt cggtttcctg acacaactgg tctatggaga cttcggaaac
     2701 gtctaacaaa atgttgagca gtgataattt atcttgtaat ttggtttcta gatgaaggtg
     2761 taacataact gaactccttt catttttata gcagaaactc gtatttattt aattatcaca
     2821 taattaaata aaacagtcaa ataattcaat ggtgacgcgt gtttaaggag tattgagaat
     2881 gtcagatata agcaaaatgc agctgaatat tcttaatttt aggtatgaaa aagcgtcggg
     2941 attgataaaa aatctattga attgggtaaa tttaagttga tataataagt tgattagaaa
     3001 aattaataat tcatttaaat tctaatcagc tgtaacgttc cggggcgtgt taaactgtct
     3061 gaacatagaa aatacaggtt tctacggtga ctaaaataag ctaaggagga ttccttaaat
     3121 tctgtgatag gaacgtaaac tctctacaag agtagagcac gtcatcagtc agtctgggtg
     3181 tctactggct gacaacgtca aataagatag gcatgaaaaa ataaggggga atacagaatg
     3241 tttttaaaac accaagatgt gaaacaaaaa aattggagaa tgcgtaaggt caaaaaagtc
     3301 tttgttagtt catgtatgct tttaacagtg ggcctcggag ttgccgtact actgcattca
     3361 gccaatctaa tggcgtgatg gtcgtaaagg ctgcggaagc tgaagaactg ccagacaatt
     3421 taattgattt taaaggaact tgggaagtat ctgccgatgg ctcttctggt cgcttctatt
     3481 cagacggtgc tacaggtcaa tataagtttc accttattcc agcttcagat gttaaaaatc
     3541 cgggatggca tgaacataat aaagttaaag atagctatgt caaaataacc aaagagtcta
     3601 ttgcagctag atatacaaat aaaacgaaac ctccatattc tgtagcgttt aaggtcaaca
     3661 ctaaatcttt aataaaagat catgactata agataacttt tgagcaaggt tcaattgcgt
     3721 cgggaataac agttgattat cgtataggca gtgcttttaa taaaactaaa gatgattctt
     3781 tcaacatttc agatgagagt aaatatgctt ctaaggtaac aatagaaggt gaggaaagag
     3841 gttttataaa taggaaacca ggaactaaaa caatatcttt tagagctgta gaaaaaggaa
     3901 ctaaatctct tgtattgtta tctaaagtta atgaaaaaac ccaagtagat cttgatgtcg
     3961 agtttaaaaa cttcaaaatt attgatgtta ccaacccaag ccaattagat aaaggagttg
     4021 cttacgttgg aaacaaaaat gtagaattga cattaaaatc agacgatgga agaacaaact
     4081 tcgaaggtga tgagataagc ctttttaagc ctaatggaga tttgttaaag aaaattgaag
     4141 ttaaagaagg acaaaaaaat cctatatcaa taactttatc tgaagatcaa gccaaatctt
     4201 taaaaaacaa agaaaagtta aaagtaagca ttaaacaaaa gcaatcaaaa aagacttcta
     4261 aagacttttt ctttgaggta ggaattgatc ctaaagttaa agcaaagcaa caagaaaaac
     4321 tcttagaact agacaaagta aaaaaacaaa ttgaagactc aatcaatggt gatgcatggc
     4381 taccagaaaa atcggaaggc gaaaaaccag tacaaaatac taataaagaa ttacagttac
     4441 aagaactgaa caagaagtat cagatggcca aagaggcaat tgaaagtgct acaactcttg
     4501 acgatgttga aactcagttt gataaataca caaaagttgg agataaagat aaatacccgg
     4561 attctttacg taaccaatat actcgaggag ataaagataa agaaatagaa aaagctaaaa
     4621 aatcactggg tgatttaagc gataaagtga atggaaagat tgaagaagat aagtggctat
     4681 ctgacgaagt taaaaaaaaa caacagcaag aattagaagc gcgtaaacaa aaagtaaacg
     4741 actctcttaa aggatctgat agtttaaaat cattgagaga aacagtggaa aaagcttctt
     4801 ctaaaaatca gaaaaaacca gaatcttttg aagatgttta tgttcctgga aatgaagaaa
     4861 ctgaaaaaac aaaagttcgt gatatacttc aaaaaactta tcaaaaaaca gagcaaaata
     4921 ttgaaacaga tccatggttg agtccggaac agaaaaaagc tcaaaaagaa aacgctaaag
     4981 caagattaga tgcaggtttg aaagctgtcg aaactacaga aagcttagat aaacttaaag
     5041 aagttgagag tgacttcctt gataaagaaa aaaatccaga ttcaattcca aatcaacata
     5101 aagctggaac tcctgaagtt gctcgcaaaa ctttcttaga taactttgaa aaagaagcca
     5161 aaaaagagat tgaatctatt gataaagatg ataccttaac ggcaaatgct aaacaagtag
     5221 ctaaagataa agtggcacaa cagctacaag aagctacagc taaagtagag aaagcacagt
     5281 cgtttgatga tttgaagaac attcaagata agttcaattc tgaattacca cacacaccag
     5341 gtaagccgct taaagatcaa caatctgatg ctattgcaga attagagaag aagcagcaag
     5401 aaattgaaaa agctattgag ggtgataaaa cattaccaag agacgaaaaa gagaaacaaa
     5461 ttgctgactc taaggaacgc ttaaaatctg acacgcaaaa agttaaagat gctaaaaatg
     5521 ctgatgctat taaaaaagca tttgaagaag ggaaagtgga tattcctcaa gcacatatcc
     5581 caggtgattt gaacaaggat aaagaaaaac ttcttgcaga gttgaagcaa aaagcagatg
     5641 atactgaaaa agcgattgat tccgataaaa ctttgacagc gaaagaaaaa gaagagcaaa
     5701 aagcgaaaac aaaagaagaa cttcaaaaag caacagaagc tgttggagca attgacaatc
     5761 gtgaagaact agataaaaaa gtcccagaac ttaagaaagc tattgaagac actcacgtta
     5821 aaggtaatct tgaaggtatt aagaataagg ctattgaaga tcttaaaaaa gctcatactg
     5881 aaacagttgc taaaataaat ggtgatgata cccttgacaa agctactaaa gaagctcaag
     5941 tgaaagaagc tgacaaagct ttggcagcag gtaaagaagc tatcactaaa gcagatgatg
     6001 ctgataaagt aagtacagct gttacagagc acacaccaaa aattaaagca gcacataaaa
     6061 ctggtgatct taaaaaagct caagtagatg ctaacacagc tcttgacaaa gcagctgaaa
     6121 aagaacgtga agaaatcaat aatgatatca cattaacggc aaaagacaaa gaacaacaac
     6181 tgaaagaagt tgagacagct cttactaaag ctaaagataa cgtgaaagct gctaagacag
     6241 cagacgctat caatgacgca cgtgataaag gcgtagcaac aattgatgcc gttcataaag
     6301 caggtcaaga cttaggtgct cgtaagtcag gtcaagtcgc taaacttgaa gaagcagcta
     6361 aagcaacgaa agacaagatt tcagctgatc caactttgac aagcaaagaa aaagaagagc
     6421 aatctaaagc tgttaacgct gaacttaaga aagcgataga agctgttaac gcagctgaca
     6481 cagctgacaa ggttgacgat gctcttggta aaggtgt
//
LOCUS       DAUVUY010000091         6481 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.091, whole genome shotgun sequence.
ACCESSION   DAUVUY010000091 DAUVUY010000000
VERSION     DAUVUY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.091"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1033
                     /locus_tag="VOX58_001641"
     CDS             <1..1033
                     /locus_tag="VOX58_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HES5878854.1"
                     /translation="TKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDL
                     SFLDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPEAREAALKAADKLVQRYQDKGGF
                     IQAWGELGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDA
                     SAYHTFYFDPETGDPIKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFK
                     GMTHYFMNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDPDKKT
                     YEAAMHSMLRALIKDYANKNLNPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLR
                     FYKDWNPYW"
     gene            1069..1557
                     /locus_tag="VOX58_001642"
     CDS             1069..1557
                     /locus_tag="VOX58_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HES5878855.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQSIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHHVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            1576..2358
                     /locus_tag="VOX58_001643"
     CDS             1576..2358
                     /locus_tag="VOX58_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HES5878856.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGMILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVVIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWAFAFMGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            2345..3166
                     /locus_tag="VOX58_001644"
     CDS             2345..3166
                     /locus_tag="VOX58_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HES5878857.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAIVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVILTVIIGVIGSWLGILA"
     gene            3252..5159
                     /locus_tag="VOX58_001645"
     CDS             3252..5159
                     /locus_tag="VOX58_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HES5878858.1"
                     /translation="MSLAFTRFKETVNPNFCRNYLLDYQADSYADRKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVTDDPEWNFMLNRQTYLQTLILVYLVERDERYL
                     LTAKGFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFDALTKQEERLIL
                     ASLEKQLHFLHANYLDKYSLSNWGILQTTAILLADAYFGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAACPTLDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSICLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQINSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGMFLIIDTIQAQGNHCLVSQFILDNHLEVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKFIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            5223..6218
                     /locus_tag="VOX58_001646"
     CDS             5223..6218
                     /locus_tag="VOX58_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HES5878859.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(6302..>6481)
                     /locus_tag="VOX58_001647"
     CDS             complement(6302..>6481)
                     /locus_tag="VOX58_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HES5878860.1"
                     /translation="AFVALMATVVVPGFNQFFHVTHLTITQWLVVIIGSLLMVVLVEL
                     VKAVQRSLGQDEKAI"
BASE COUNT         1963 a         1415 c         1190 g         1913 t
ORIGIN      
        1 taccaaagat aaccaatatg ccattatgga taatacagaa tggaccaatg ccttttggac
       61 aggttgcctt tggttagctt acgaatacag tggggatgat gctattaaag ctctggctca
      121 agcaaatgac ctttctttcc tagatcgagt aacaagagac attgagctag atcaccatga
      181 tttgggcttt ctttacaccc cttcttgcat ggctgagtgg aaacttttaa aaacacctga
      241 ggctagagaa gctgctttga aagcagccga caaattagtc caacgctatc aagacaaagg
      301 cggctttatt caggcctggg gagaactggg taaaaaagaa gattaccgtt taatcattga
      361 ttgtctccta aacatccaat tactcttttt tgctagccaa gaaactggag acaaccgtta
      421 ccgtgacatg gcgatcaatc atttctacgc ctcagccaat catgtcatcc gagatgacgc
      481 ctctgcttac cacaccttct actttgatcc agaaacaggc gatcctatta aaggggtcac
      541 ccgacaaggt tatagcgatg attctgcttg ggccagaggg caagcttggg gcatttatgg
      601 tattcctttg acctatcgtt tcctaaaaga acctgaactc attcaactct tcaagggcat
      661 gacccattat ttcatgaacc gtcttcctaa agaccaggtt tcctactggg atttaatttt
      721 tggagatggt agcgagcagt ctcgtgatag ttcagcaacc gccattgctg tatgtggtat
      781 tcatgaaatg ttaaaaacct taccagatca tgatcctgat aaaaaaacat atgaagctgc
      841 catgcacagc atgctaagag ccttgattaa ggattatgct aacaaaaatc tcaatcctgg
      901 cgctcctcta ctacttcacg gagtctattc ttggcactct ggaaaagggg ttgacgaggg
      961 aaatatctgg ggagactatt actatcttga agctctgctt cgtttctaca aagactggaa
     1021 cccttactgg taacaaacac cgataccgac atttaaaaag gagattttat gacacaacca
     1081 aacattatca tgacgcgagt tgatgagcgc ttgattcacg gacaggggca attatgggtc
     1141 aaattcctca actgcaacac cgttatcgtc gctaacgatg cggtttctga agataagatt
     1201 caacaatccc tcatgaaaac agttattcca tccagtattg ccattcgttt tttctccatt
     1261 caaaaagtga ttgacatcat tcataaagca agtcctgctc aaagtatctt catcgtggtc
     1321 aaagatttgc aagatgccaa acgcctagtt gaagggggtg ttcccatcac agaaatcaat
     1381 atcggtaata ttcataaaac tgatgataag gttgcgatta cccagtttat ttcgctggga
     1441 gaaacagaca agtctgctat tcgttgctta gcccatgacc accacgtggt ttttaacacc
     1501 aaaacaactc cagctggcaa tagcgcctct gacgttgaca tcttagatta tatttaatga
     1561 ataaaggaga ttgatatgga tatcaatttg ttacaggcac ttttaattgg tctttggaca
     1621 gccttttgtt tcagcggaat gttacttggc atctacacca accgttgtat tattctgtct
     1681 tttggtgtag ggatgatctt aggtgacttg ccaactgcac ttagcatggg agctatctcc
     1741 gaattagctt atatgggatt tggagtgggt gccggtggta ccgttcctcc taatcctatc
     1801 ggccctggta tctttggtac cttgatggct attaccagtg ctggtaaagt taccccagaa
     1861 gcggccctag ccttatcaac accaatcgca gttgtcatcc aatttcttca gacattcgcc
     1921 tataccgctt ttgctggggc tcctgaaacc gctaaaaaac aattgcaaaa aggcaatatt
     1981 agaggcttca aattcgctgc caacggcact atctgggctt tcgcttttat gggattaggc
     2041 cttggtttat taggtgcctt gtcaatggat accctccttc acttggttga ttatatccca
     2101 cctgtcttgc tcaatggatt gactgttgct ggtaaaatgt tacctgctat cggatttgcc
     2161 atgatcttat cagttatggc caagaaggag ttgattcctt ttgtactgat tggttatgtt
     2221 tgtgcagcct acctccaaat tccaaccatt ggtatcgcca ttattggtat cattttcgcc
     2281 ttgaatgaat tttacaacaa acctaaacaa gtcgatgcaa caactgtcca aggaggccaa
     2341 caagatgact ggatctaata aattaaccaa gaaagattac ctcaaaacag ctctgagagc
     2401 tttcttccta caaaatgggt ttaattacag taattaccaa ggtatcggat acgccaatgt
     2461 catctatcct gccttgaaaa aacattttgg cagtgataaa aagggcttat atcaagcttt
     2521 agaagataac tgtgagtttt acaatactaa ccctcacttc cttcctttca tcaccagtct
     2581 gcacttggtc atgttagaaa atgatcgtcc tgaagaagaa acccgaaaca tcaaaatggc
     2641 acttatgggg cctttagctg gtatcggtga ctccttatca caattttgct tggcgccact
     2701 cttttcaacg attgcagctt ccttagctag tgatggttta gtgttaggtc caattttctt
     2761 cttcctagcc atgaatatta tcctaactgc tattaaaatc ggttctggtc tctatggcta
     2821 caaggtcggc acaagcttta ttgataaact aagcgaacaa atggcgatag tatctcgtat
     2881 ggcaaatatt gtcggtgtta ccgttatcgc tggattagct gctacttctg ttaaaataac
     2941 tgttcccatt acttttgcgg ctggcaaggt ggatgcggcc aatacagcac aaaaatttgt
     3001 gactattcaa ggaatgcttg ataagattgc ccctgcactt ttaccagctc tctttacctt
     3061 actcatgtat taccttatta aaaacaaaaa atggacaacc tacaaattgg tcattttaac
     3121 tgttatcatt ggtgttattg gcagttggtt gggtatttta gcttagttgt cactgagatt
     3181 ctgttatggg tttaggaact atttcctagt cacattaaca ggtctctttt cttttggaaa
     3241 ggaaacgtcc tatgtctctt gccttcacac gctttaaaga aacagtcaat ccaaactttt
     3301 gcaggaatta cctgctagac tatcaggctg acagctatgc tgatcgaaaa aggatagctg
     3361 acctactctt aaccaatacc tttttatttg aagataattg ggatatggag ccctgccaca
     3421 tcccttacca cttagatccc attacttggc aagaggctgt cacagatgat cctgagtgga
     3481 attttatgtt aaatcgacag acctatctcc aaacattaat cctcgtctac cttgttgaaa
     3541 gagatgaacg gtatctctta acagctaaag gtttcattct caattggatt gaatcagcta
     3601 ttcctttaga tcctaagggg ttagctacta gaaccttaga tactggcatc cgttgttttg
     3661 cttgggtcaa atgtttgatt taccttaatc tttttgacgc cttaaccaag caagaggaaa
     3721 ggctcatcct agcttccctg gaaaaacaac ttcactttct ccatgccaac taccttgata
     3781 aatacagtct cagcaattgg ggaatcttgc aaaccactgc gattttatta gctgatgcct
     3841 actttggttc tgaccttgat attgctgcag caacagcttt cgctagaaaa gaattgacgc
     3901 aacaaatcgc actccaaatt ctcgaagacg gcagtcaatt tgaacaatca accatgtatc
     3961 atgtggaagt actcaaagcc cttctggaat taactgctct agtccctgat tatcttccac
     4021 agcttagacc tactttgctt gccatgagtg attatctcct caaaatgact ggccctgacc
     4081 acaaacagat ccccttagga gacagtgact ttaccgatac cagagatatt ctaacactag
     4141 cagccactat ccttgaagaa ccacacttaa aagctgctgc ttgtcccaca cttgatatcg
     4201 atagtcttct ccttctagga gaaaaaggtg tccacacttt tgaacagtta cccgtccaaa
     4261 ccttaccaac ttttgcgcat cactttgaac attccgggca cattaccatc aatcaagaaa
     4321 attattatct ttttttcaaa aatgggccca tcggtagttc ccatacccat agcgaccaaa
     4381 atagtatctg tctctattat aaaggacaac cacttttctg tgatgctggg cgatacactt
     4441 acaaagaaga accattacgc tatgccctaa aaagcgctag ccatcattct accgccttct
     4501 tagaagaaca attacctgag cagattaata gctcctgggc ttacctttct taccccaaat
     4561 ccaattattg tcaccttcga caaaatggac acgtttactt tattgaaggt agctaccaga
     4621 cgcaattctc tgataggaac aactaccaac atgaccgaca aattctcatc ttaccaccag
     4681 gaatgtttct tatcattgat acgatacagg ctcaaggtaa ccactgtctt gtcagtcaat
     4741 ttattcttga taatcactta gaagtaaaga cagatcacct tagtgacctc agactaatca
     4801 gtgactgccc tttcactatc gaagaaacga ttttgtcaaa aaaatacaac caatacctga
     4861 ctagtcacaa attcatcaaa cgaaaaccct ttaaagacaa gggttgcacc agtaccctgt
     4921 tagtccctga cgataccaaa gttactccct taacaccact tcaaacaggc aaacgcaacc
     4981 caattgagac cgccctttcc tggcatttaa aaggaaagca gtttgactac agtatctgcg
     5041 ttctccaaga ggacttaatt aaaggagaaa aactagttct cctaaatggt cataaaatca
     5101 gaggaaaggt ggttgttatc aaccatatca cgaatgagat tattcgattg aaacactaaa
     5161 tcttttacga gtccttatgt tataatatga ctaaccatta ctattttaaa ggagtgtatg
     5221 caatgcagcg caaagtaacc atcaaagata ttgctgaatt agcgaaaact tcaaaaacaa
     5281 cagtttcttt ctacttaaat ggtaggtttg acaagatgtc tgaagaaact aaaaaccgca
     5341 ttaaaagcac gattgaagct accaattata aaccaagcat tgctgccaga agtctaaacg
     5401 ccaaatctac taaactcatt ggcgtggtta ttggtgatat taccaatagt ttttccaatc
     5461 aaattgttaa gggaattgaa agcaaagccc aggaatttgg ttaccaaatt atcatcggaa
     5521 acagcaacta cgacccttct cgcgaagatg aactcattga aaagatgctg aacctcggag
     5581 tggatggctt tatcattcag cctacctcta atttcaggaa gtattctcgc attatcgata
     5641 ttaagaagaa aaaagtggtc ttttttgata gccaacttta cgaacatcgc acaaactggg
     5701 tcaaaactaa taattatgat gctgtttatg ataccattca acaatgtatc gataaaggtt
     5761 atgaacattt tatcatgatc acgggtaacc ccaatttact tagcactcgt attgaacgcg
     5821 cttcaggctt cattgatgtg ttagaagcta atcatcttac tcatcaagaa atgattattg
     5881 atgaaaatca gacttcttca gaggcaatcg ctcagttttt gcaaggatca ctgactaaaa
     5941 agagtttggt ttttgtcccc aattgttggg ctcttccaaa agtatttaca gctatgaagt
     6001 ctcttaaatt taatatacca gagattggac tagtaggatt tgataacatt gagtggacaa
     6061 aattttcttc accaacacta acaactatta tccaacccgc ctatgaagaa ggcgaacagg
     6121 ctactaagat tttaatcgat gatattgaag gtcacagtca agaagccaaa caacaaattt
     6181 ttgactgtca agtcaattgg caagaatcaa ctttttaatg tcaggcatac caatagtcct
     6241 tatcgttgta acgattataa aaaaaggttt gagacaattg tctcaaacct tttagcgtct
     6301 attaaattgc tttttcatct tgaccaagtg atcgttgaac agctttcacc aattcaacaa
     6361 gaacaaccat caagagactt ccgataataa ccactaacca ttgtgtgatc gtcaagtgtg
     6421 tcacatggaa gaattgatta aatccaggaa caacaactgt tgccatcaat gctacaaagg
     6481 c
//
LOCUS       DAUVUY010000092         6408 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.092, whole genome shotgun sequence.
ACCESSION   DAUVUY010000092 DAUVUY010000000
VERSION     DAUVUY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.092"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..219
                     /locus_tag="VOX58_001648"
     CDS             <1..219
                     /locus_tag="VOX58_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HES5878861.1"
                     /translation="ITTAKQMTNVRSATKDQTAVIKTPRILTLEESLEFLNDDEYMEV
                     TPESIRLRKQILNKAARDKANKKKKSAE"
     gene            341..592
                     /locus_tag="VOX58_001649"
     CDS             341..592
                     /locus_tag="VOX58_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HES5878862.1"
                     /translation="MFYLIMAVSIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            948..2306
                     /locus_tag="VOX58_001650"
     CDS             948..2306
                     /locus_tag="VOX58_001650"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HES5878863.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDEDFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            2306..3388
                     /locus_tag="VOX58_001651"
     CDS             2306..3388
                     /locus_tag="VOX58_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HES5878864.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIVAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            3389..4537
                     /locus_tag="VOX58_001652"
     CDS             3389..4537
                     /locus_tag="VOX58_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HES5878865.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMYDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPSPETVLEQAHG"
     gene            4749..6113
                     /gene="ftsA"
                     /locus_tag="VOX58_001653"
     CDS             4749..6113
                     /gene="ftsA"
                     /locus_tag="VOX58_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HES5878866.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            6137..>6408
                     /locus_tag="VOX58_001654"
     CDS             6137..>6408
                     /locus_tag="VOX58_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HES5878867.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILT"
BASE COUNT         2105 a         1094 c         1365 g         1844 t
ORIGIN      
        1 attacaacag ctaagcaaat gacaaatgtc cgttcagcga ctaaagatca aactgcagtt
       61 atcaagacgc ctcgtatcct aacgcttgaa gaatcattag agttcttgaa tgacgacgaa
      121 tacatggaag taacgccaga atctatccgc ttgcgtaaac aaatcttgaa taaagctgcg
      181 cgtgataaag ctaataaaaa gaaaaaatca gccgaatagc ttttgaagtt aaactaacta
      241 aataaaatag caaaaagata tttaagaaaa gtcatttttt aaaggtcttt ttgcgtttac
      301 aagttcctat ggaatataaa ccaaaataaa ggaggacgga atgttttacc ttattatggc
      361 tgtctcgatt atatcttatt atctatacat ggctcctaaa agtgtccgta acacccttgg
      421 gatgattggt ttagttggct tggttgctct gcttattgtt ttagcgggct tgagttttat
      481 caaaatcatg caaacccctc ctgaattttt tattgggatg ggaatggtgg cattgggcta
      541 ttttgcttta aaagatgtgc gtaaaatgac caaaaaaccg agagttaaat aaaaatactt
      601 aaagattgat gattttggtt tttatacgat aaaaggactg atttgtttta gacaatatca
      661 gtcctttttt aatagttcat tttttctgaa agaaagcttt tagccgattt tcattgagtg
      721 tgcaactaaa aaaagatgct tggtagtgac agttaagacc tctgactagt attttatctt
      781 actttactta aaaaaacgta ccaataatct cacaaatatg ggcatgatgt ttgtatattg
      841 gaaacggatt ctaattctaa actcaagaac ggtattttta aaatgttgaa aaagcatcta
      901 aataaaggta aaatagaaat caaatagaat ggatagtgtg agcttagatg aaagtgataa
      961 gtaattttca aaataaaaaa atattaatat tggggttagc caaatcgggc gaagcagcag
     1021 caaaattatt gaccaaactt ggtgctttag tgactgttaa tgatagtaaa ccatttgacc
     1081 aaaatccagc ggcacaagcc ttgttggaag aggggattaa ggtcatttgt ggtagccacc
     1141 cagtagaatt attagatgag gactttgagt acatggttaa aaaccctggg attccttatg
     1201 ataatcctat ggttaaacgc gcccttgcaa aggaaattcc catcttgact gaagtagaat
     1261 tggcttattt cgtatctgaa gcgcctatta tcgggattac aggatcaaac gggaagacaa
     1321 ccacaacgac aatgattgcc gatgttttga atgctggcgg gcaatctgca ctcttatctg
     1381 gaaacattgg ttatcctgct tcaaaagttg ttcaaaaagc aattgctggt gatactttgg
     1441 tgatggaatt gtcctctttt caattagtgg gagtgaatgc ttttcgccct catattgctg
     1501 tcatcactaa tttaatgccg actcacctgg actatcatgg cagttttgag gattatgttg
     1561 ctgctaaatg gatgattcaa gctcagatga cagaatcaga ctaccttatt ttaaatgcta
     1621 atcaagagat ttcagcaact ctagctaaga ccaccaaagc aacagtgatt cctttttcaa
     1681 ctcaaaaagt ggttgatgga gcttatctga aggatggaat actctatttt aaagaacaag
     1741 cgattatagc tgccactgac ttaggtgtcc caggtagcca caacattgaa aatgccctag
     1801 caactattgc agttgccaag ttatctggta ttgctgatga tattattgcc cagtgccttt
     1861 cacattttgg aggcgttaaa catcgtttgc aacgggttgg tcaaatcaaa gatattacct
     1921 tctacaatga cagtaagtca accaatattt tagccactca aaaggcttta tcaggttttg
     1981 ataacagtcg cttgattttg attgctggcg gtctagatcg tggcaatgaa tttgacgatt
     2041 tggtgccaga ccttttagga cttaagcaga tgattatttt gggagaatcc gcagagcgta
     2101 tgaagcgagc tgctaacaaa gcagaggtct cttatcttga agctagaaat gtggcagaag
     2161 caacagagct tgcttttaag ctggcccaaa caggcgatac tatcttgctt agcccagcca
     2221 atgctagctg ggatatgtat cctaattttg aggttcgcgg ggatgaattt ttggcaacct
     2281 ttgattgttt aagaggagat gcctaatgcc taagaagatt ttatttacag gtggtggaac
     2341 tgtaggtcat gtcaccttga acctcattct cataccaaaa tttatcaagg acggttggga
     2401 agtacattat attggtgata aaaatggcat tgaacataca gaaattgaaa agtcaggcct
     2461 tgacgtgacc tttcatgcta tcgcgacagg caagcttaga cgctattttt catggcaaaa
     2521 tctagctgat gtttttaagg ttgcacttgg cctcctacag tctctcttta ttgttgccaa
     2581 gcttcgccct caagcccttt tttccaaagg tggttttgtc tcagtaccgc cagttgtggc
     2641 tgctaaattg cttggtaaac cagtctttat tcatgaatca gatcggtcaa tgggactagc
     2701 aaacaagatt gcctacaaat ttgcaactac catgtatacc acttttgagc aggaagacca
     2761 gttgtctaaa gttaaacacc ttggagcggt gacaaaggtt ttcaaagatg ccaaccaaat
     2821 gcctgaatca actcagttag aggcggtgaa agagtatttt agtagagacc taaaaaccct
     2881 cttgtttatt ggtggttcgg caggggcgca tgtgtttaat cagtttatta gtgatcatcc
     2941 agaattgaag caacgttata atatcatcaa tattacagga gaccctcacc ttaatgaatt
     3001 gagttctcat ctgtatcgag tagattatgt taccgatctc taccaacctt tgatggcgat
     3061 ggctgacctt gtagtgacaa gagggggctc taatacactt tttgagctac tggcaatggc
     3121 taagctacac ctcatcgttc ctcttggtaa agaagctagc cgtggcgatc agttagaaaa
     3181 tgccacttat tttgagaaga ggggctacgc taaacaatta caggaacctg atttaacttt
     3241 gcataatttt gatcaggcaa tggctgattt gtttgaacat caggctgatt atgaggctac
     3301 tatgttggca actaaggaga ttcagtcacc ggacttcttt tatgaccttt tgagagctga
     3361 tattagctcc gcgattaagg agaagtaaat ggcaaaagat aaagagaaac aaagtgatga
     3421 caagctcgtt ttgacagagt ggcaaaagcg taacattgaa tttttaaaga aaaagaagca
     3481 gcaagctgag gaagaaaaaa aactcaaaga gaaattattg agtgataaaa aagcgcagca
     3541 gcaagctcaa aatgcttctg aagcagttga gcttaaaact gatgagaaaa ctgatagtca
     3601 ggaaattgag tcagaaacga cgtcaaaacc taaaaaaccc aaaaaagtta gacaacccaa
     3661 ggaaaaaagc gcgacacaaa tcgcttttca aaaatccttg cctgttcttt tgggggcgct
     3721 cttacttatg gcggtgtcta tttttatgat cactccttat agcaaaaaga aagagttttc
     3781 tgtaagagga aaccatcaaa cgaaccttga cgaattaatc aaagctagca aagtcaaagc
     3841 atctgactat tggttaacgt tgttaacttc gcctggtcag tatgaacgac cgattcttcg
     3901 tactattcca tgggtgaaat ctgtacatct ctcttaccaa tttcctaatc actttctatt
     3961 taacgttatt gaatttgaaa tcatcgctta tgcacaagtc gaaaacggtt ttcagcctat
     4021 tttggagaat ggaaaacgtg tggacaaggt cagggcatca gaactaccga aatctttctt
     4081 gattcttaat ttaaaagatg agaaagcgat ccaacagtta gttaagcaat taacgacatt
     4141 acctaaaaaa ttagtcaaga atatcaagtc agtgtctctt gcaaattcca aaacgacagc
     4201 ggatttacta cttattgaaa tgtatgacgg taatgtagtt agagtaccgc agtcacaact
     4261 cacattgaaa cttccctatt atcaaaaatt gaaaaaaaac cttgaaaatg atagtatagt
     4321 ggatatggaa gttggaattt acactacaac acaggagatt gaaaatcaac ctgaagttcc
     4381 tcttacgcca gaacaaaacg cagctgataa agaaggagat aagcctggtg agcatcagga
     4441 acagacagac aatgattcag aaacgccagc aaatcagagt agtcctcagc aagcaccacc
     4501 atccccagaa acggtcctcg aacaggccca tggctagcta atatctaagt tgaaaaagca
     4561 atgaaaacgt tagaaattca acgattctaa cccataatga attgcctaaa aaaaattaag
     4621 tttatataac aaaaaacgta aaatgataac attttacgtt tttttatggt ataatatttt
     4681 ctgaatgatt ctgtttttta gcagttttta gaatagcaaa agtttggaaa gtaagtgagg
     4741 tcaagtgaat ggctagaaat ggctttttta ctggtttgga cattggaaca agctcgataa
     4801 aagttttagt agcagaattt atttctggtg agatgaacgt cattggtgtt agtaatgttc
     4861 caagtaccgg cgtaaaagat ggcataataa tcgatataga ggcagctgcg actgccatca
     4921 aaactgcggt agaacaagca gaagaaaaag cagggatgac aattgaaaag gttaatgttg
     4981 ggctaccggc aaaccttctt caaattgaac caacacaagg aatgattcct gtcccaagtg
     5041 agtctaaaga gataaaagat gaggatgttg atagcgttgt taaatcggct ttaacaaaaa
     5101 gtatcacacc agaacgagag gttatctctt tagttccaga agagttcatt gtggatggct
     5161 ttcagggcat tcgagatcca cgtggtatga tggggattag attagagatg cgcgggctta
     5221 tttatactgg accaagcacc attttacata atctgcgtaa aacggtagaa agagcaggca
     5281 ttaaagttga aaacatcatt atttctccgt tagctatggc taaaaccatt ttaaacgaag
     5341 gtgagcgcga gtttggagct actgtaattg atatgggagg tggacagaca actgtcgctt
     5401 ctatgcgagc acaagaattg cagtatacca atatatatgc tgaaggcggc gaatacatta
     5461 ctaaagatat atcaaaagta ttaaaaacgt ctttggctat tgcagaagca cttaagttta
     5521 attttggtca agcggagata tcagaagcta gtataactga aacagtaaaa gttgatgtgg
     5581 taggtagtga agagcctgtt gaggtaactg aacgttattt atctgaaatt atttcagcgc
     5641 gtattcgtca tattttagat cgtgtgaagc aagatttgga aagaggtcgt ttactagact
     5701 taccaggagg cattgttttg attggtggcg gtgcaatcat gcctggagtg gtagaaattg
     5761 cacaagaaat ctttggagta actgtaaagc tccatgttcc aaatcaagtc ggtattagaa
     5821 atccaatgtt ttcaaacgtt atcagtttgg tagaatatgt tggtatgatg tctgaagtag
     5881 acgttttagc acaaactgca gtttcaggag aagaactttt gcgacgcaag cctatcgatt
     5941 tcagtggtca agaatcttat ttaccagatt atgatgattc aagaagacca gaatcgacca
     6001 ttggctatga acaacaagcg tcacaaacag catatgattc acaagttccg agtgatccta
     6061 aacaaaaaat ttcagaacgt gttcgtggca tatttgggag tatgtttgat taaaagtaat
     6121 aaagtgagga gataaaatgg cgttttcatt tgatactgca tcaattcaag gtgcaattat
     6181 aaaagtaatt ggagtcggcg gaggtggcgg aaatgccatt aatcgtatga ttgatgaagg
     6241 tgttgctggt gtcgagttca tcgcagcaaa tacagacatt caggcattaa gctcatcaaa
     6301 agctgaaacg gttattcaac taggccctaa attaactcgt ggacttggtg ctggaggaca
     6361 acctgaagta ggacgtaaag ctgctgaaga aagcgaagaa attttaac
//
LOCUS       DAUVUY010000093         6289 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.093, whole genome shotgun sequence.
ACCESSION   DAUVUY010000093 DAUVUY010000000
VERSION     DAUVUY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.093"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(54..113)
                     /gene="ermCL"
                     /locus_tag="VOX58_001655"
     CDS             complement(54..113)
                     /gene="ermCL"
                     /locus_tag="VOX58_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HES5878868.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            562..2064
                     /locus_tag="VOX58_001656"
     CDS             562..2064
                     /locus_tag="VOX58_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HES5878869.1"
                     /translation="MSYLVARMQKMKVGNLGGAYRHNERVFENHSNKDIDTSRSHLNY
                     ELTDRDRSVSYEKQIKDYVNEKKSSKRAIRKDAVLCDEWIITSDKDFFERLDQEQTRA
                     FFETAKNYFAERYGLENIAYASVHLDESTPHMHMGVVPMVDGKLSSKAVFTREELKAI
                     QEELPKYMNEQGFELSRGQLGSDKKHLSVADYKAKIGKEALNKELLGLGAPRYWHKEE
                     DRPATAEEIAGYESLASLFAGEEMKLREATLEERFTWLDSHRNDLKGDLSHLEELVDK
                     KIEEYTRIDSEASERLSELSELNSKVKDREKELRGLESDSERLSDKVVRLEKEHRETT
                     QLLVEQNRNLRKISFQDLDRRRIAEDLHEELEKATPKLFGGSFNFTADFVGRLKTFMS
                     EVVEKLEQVINQNEVLRKALEGMKQAKERAERELLQEEWKTQRLETENQNLRQENKEL
                     KVSKNLLEDIQEVITEKEVSSLNKRLDELREARMASKRGYEPKRSKGRSI"
     gene            2780..2926
                     /locus_tag="VOX58_001657"
     CDS             2780..2926
                     /locus_tag="VOX58_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HES5878870.1"
                     /translation="MAKKRMGISLSEEVAEMLEKQAKEAGLNKSALITTLIVKENKNQ
                     DRKV"
     gene            2981..3646
                     /locus_tag="VOX58_001658"
     CDS             2981..3646
                     /locus_tag="VOX58_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HES5878871.1"
                     /translation="MAKEKSRNFTFLLYPDGDGFPSDWEERLEKIGVPIAISPLHDKD
                     KNKNRTLKKPHYHGIYIANNPVTAESVKNKLRAVLSSEDVECKAVAKVQIIYESIESV
                     YLYLTHESKDAIKKNKYRYDKADIKHINNFDIDRYVVVDVETKNQILKILLQIIRAYS
                     IPNVLDLHDFIEENGSSYGIDMNLFLTTIESKSSILRLYFDGAYQRGQRGLKSGEEKN
                     GNI"
     gene            3633..3839
                     /locus_tag="VOX58_001659"
     CDS             3633..3839
                     /locus_tag="VOX58_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010010243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878872.1"
                     /translation="MEIFDKYYDEHNLEERSQYSGFSKKQLVIEAEHLENALSRVLVY
                     LENNGDDLDFIRTEVMDGLYESRI"
     gene            complement(4227..4961)
                     /locus_tag="VOX58_001660"
     CDS             complement(4227..4961)
                     /locus_tag="VOX58_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HES5878873.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
     gene            5069..6259
                     /locus_tag="VOX58_001661"
     CDS             5069..6259
                     /locus_tag="VOX58_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HES5878874.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFKGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGAFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT         2064 a         1016 c         1405 g         1804 t
ORIGIN      
        1 ccactcctat gaggttataa tgaatgttta tattaacaat ccattataac cacttatttt
       61 ttgtttggtt gataatgaac tgtgttgatt acaaaaatac taaaaatgcc catatattaa
      121 ttctcctttt tatgaaatta gaataattat agcacaagct ctgataaata ataacatgat
      181 gaacgactgt taaatttata ctgcaattgg gccctattgt tgaataaaga tacgagagat
      241 ttctcttgta tcttttttat ttttgagtgg taacgaaagc atcccccctt tgctgaggtg
      301 gcagaggtcg ggtttttttg tttctttttt tacgaaaaaa aaaggtctta aaaggtttta
      361 tggttttggt cggcactgcc gacagcctcg cagagtacac acttatatga atataaagta
      421 tagtgtgtat actttacatg gaagttgtgc cgaaaatgtg ctaaactact agctaagatg
      481 ttaggtattt aggcaagcaa tagcctgggc ggcttgcctg tcattcgcta ttcgaatgac
      541 cacctgggaa ggaactgaag gatgagttat ctcgtagcaa gaatgcaaaa aatgaaagtc
      601 ggaaatcttg gcggagctta taggcacaat gaacgtgttt ttgagaatca ctcgaacaaa
      661 gatatagaca cgagcaggtc gcacctcaac tatgagttga cggatcgcga ccgctctgtg
      721 tcgtatgaaa agcagattaa ggattacgtg aatgaaaaga agagctctaa gcgagctatt
      781 cggaaagatg cggttttatg tgatgagtgg attatcacgt ctgataaaga cttttttgag
      841 aggttagacc aggagcagac cagggcattt tttgagactg ccaaaaatta ctttgctgaa
      901 cgttatggct tagaaaatat tgcttatgct agcgtacatt tggacgagag tacccctcac
      961 atgcatatgg gagtagtacc tatggtggac ggaaaattgt cgtctaaagc tgtctttacc
     1021 cgtgaagaat taaaggctat tcaagaagaa ctgccaaaat atatgaatga gcagggtttt
     1081 gagttgtcac gtgggcaatt aggaagtgat aagaaacacc ttagtgtagc tgattacaag
     1141 gctaaaattg ggaaagaagc tctgaataaa gaattattag ggctgggtgc tcctcgttat
     1201 tggcacaaag aggaggatag accagcaact gcagaagaga tagcaggtta tgaaagttta
     1261 gcctcgttgt ttgctggaga ggaaatgaaa ctacgtgaag caacgttgga agagcgtttt
     1321 acatggctag atagccatag aaacgattta aagggcgatt taagccattt agaggagtta
     1381 gtggataaaa agattgaaga atatactaga atcgattctg aggcctctga gaggctctca
     1441 gagctatctg agttaaattc caaggtaaaa gaccgagaaa aagaattaag gggcttagag
     1501 agcgattctg agcgtctgag cgataaagtg gttcgtttgg aaaaggaaca tagggaaacg
     1561 acacaacttt tggtcgaaca aaacaggaat ttgcggaaga tttcgtttca agatttggat
     1621 agacgtagaa ttgcagaaga tttgcatgaa gagttagaga aagcgacccc taaattgttt
     1681 ggtggtagtt ttaactttac ggcagatttt gttggtcgac tcaagacttt tatgtctgaa
     1741 gttgttgaga aactggaaca ggtaatcaat caaaacgaag tgttgaggaa agcgttagaa
     1801 ggcatgaagc aagctaaaga gagggcggaa agagaattat tgcaggaaga atggaaaact
     1861 caaagactag agacagagaa tcaaaatttg agacaagaga acaaagagtt aaaggtttct
     1921 aaaaacctct tagaagacat tcaagaggtt atcacagaga aagaggttag tagtcttaat
     1981 aagcgtttag atgagcttag agaagctaga atggcttcta agagaggata tgaacctaaa
     2041 cgttctaaag gtcggagtat ctgagcgatt tatgccgtga aagctccttg atagcaagct
     2101 ggggcaaggt agacttaggg cgggcggttt ggtcagaact ttgttcgacc aaccggcaga
     2161 ccaagaacta ccgccacagg ttacttgctg tcaaggagaa catggaataa agtaagcgga
     2221 catgatataa taaccaatgc tgaagcaaaa ccttccgcaa gtctatgcga aaggaggtgg
     2281 agcgagtgca ttacattttc gacgtcttac ttgctattgt ggcagaagta acggcacatt
     2341 acatttgcaa gtggttagat agcaaggaca aacgctgaag ctagcccaac accgacaaaa
     2401 aagcccctag ggaactggca ttccttaggg gcttcggtgt gcttgtgcac tacattttcg
     2461 taaactcatt gtatcacgtg ggaaagcacg tgtcaaaaaa tgggcacgtg aaaaaaactt
     2521 gagggggatt tttcggcaga aaaatctaaa atctgggggg ctactacgac cccccttagg
     2581 tgccgagtgc caaaacgggg taaaattagg gttcaggctt agagcctcaa ggctttagag
     2641 gttgctctct aaattcggaa attcgtattt agagagcaaa aaatagggtt tagagagcaa
     2701 atccatgata aaaaaacaaa aaatgttgca aaaatttgta aaacgtgtta caatgtcaaa
     2761 aaaagaaaaa ggtgtaatta tggcaaaaaa acgaatgggg atttcgcttt ctgaagaagt
     2821 agcggaaatg cttgaaaaac aagcgaaaga agccggtttg aataaatctg ctttaataac
     2881 gactttgatt gttaaagaaa acaagaatca agacagaaag gtataaaaaa agctgtgttg
     2941 gggacacagc ggtatttttg agttaggtta attatagcat atggcaaaag aaaaatcgag
     3001 aaattttaca tttttgctct atcctgatgg ggatgggttt ccaagcgatt gggaagaacg
     3061 gttagaaaaa atcggcgtac cgattgcaat tagtccgctt catgacaaag ataagaataa
     3121 aaatagaacg ctgaaaaaac cgcattatca tgggatttat attgcaaata atccagtaac
     3181 cgctgaatca gtaaaaaaca aattgagggc tgttctcagt tctgaagatg tggagtgcaa
     3241 agcggtagcg aaagtgcaga ttatttacga atcaattgag agtgtctatt tgtatcttac
     3301 gcatgaaagt aaggatgcta tcaagaaaaa caaatatcgt tatgacaagg cagatattaa
     3361 gcatataaac aactttgata ttgaccgcta tgttgtggtt gatgttgaaa ccaaaaacca
     3421 aattttgaaa attttgctcc agattattcg tgcttattca attccaaacg tgcttgacct
     3481 ccatgatttc atcgaggaaa atggttcaag ttatgggatt gatatgaatt tgtttttgac
     3541 gacaattgaa agcaaatcga gtatcttgcg tttgtatttt gatggagcgt atcagcgtgg
     3601 tcaacgaggg ttgaagtcag gggaagagaa aaatggaaat atttgataag tattacgatg
     3661 agcataacct agaagaacgc agtcaatata gtggatttag caaaaagcaa ttggtgattg
     3721 aagcggaaca tttagaaaat gctttgtcaa gggttttagt ttatctagaa aataacggtg
     3781 atgatttaga tttcattcgc acagaagtaa tggatggact gtatgaaagt cgcatataaa
     3841 aagctgacct ttcggggtca gctttttagt ccgacaatag cgaagcggtc ggaccgtcat
     3901 tttgaaaaaa tgactttggg cgacttgtcg aagtcgtcca aacatgacaa aaatgtcctt
     3961 gtggttcgag ggcgtttttg ttatctcgcc acctttcctt tgtggcgagc tggccgtctg
     4021 caagaccctt cggagaacgc acatttacga agtaaagtat agtgagttat actttacttg
     4081 gaagtggttg ccggaaatag tgaaaatgcc tcacatttgt gccacctaaa aaggagcgat
     4141 ttacatatga gttatgcagt ttacgggcca agattgttta agcaaaataa gataagttaa
     4201 gggatgcagt ttatgcaccc cttaacttat ctattaaata atttatagct attgaaaaga
     4261 gacaagaatt gttcaaagct aatattgttt aaatcgtcaa ttcttgcata ttttaaggct
     4321 tgataaaatt ggtttttgga aaatagtttt atgtattctt tgttaaccca tttcataaca
     4381 aaattttcat attgttttct atctttgagt gatatttttg aagggtgtct ttttaataca
     4441 attaacgagc tattaactct aggttttgga tgaaagtatt ctctagggat tttacttaat
     4501 atggatatat caacttctgt cattaaaaat agtgctagtg aacgatttgt atttagcaac
     4561 cttttagcaa atccatattc cactattaaa taactttctg tagctgtgct ttcaaaaaca
     4621 atttttcgaa ttatatctgt acttatattg tagggtatat ttccatatat cttatatgac
     4681 ttatttttag gaaacttaaa ttgtaaaata tctttattga taacctggaa gttctcatat
     4741 tcaataagtt tgtttttagt tatcctacat aatttaggat ctatttcaat ggcggttaca
     4801 taattacacc ttttagctaa ttcaaacgtg aaatgacctt tccctgaacc aatctcaata
     4861 atattatcat ttgtatttaa atgtacattt ctcaaaattt catttatatg atgctttgaa
     4921 gtaataaagt tttgactatc ttttatattt tttttgttca ttataaccca ctccgagagt
     4981 gtaaaatatt ttgtgtaaat agaaaaaagg aagtcccttc tgtagaatag agttaccaca
     5041 acacattcac agaagagagg acttccctat gaacgatttt actacagaaa ttctaaagac
     5101 tctagcgaac aaaggcgatt tgaatgaatt attccgtgtc catttggaga aagcagtcaa
     5161 tacgcttctc aaaacggagt taacggcttt cctagattac gaaaagtatg atcgcattgg
     5221 ttttaacacg ggtaattctc gtaacggctc ctatgaccgt acggtcaaga ccgagtatgg
     5281 ggaacttcat ctccagattc cgcgcgaccg caatggtgag ttcaagcaac agactgttcc
     5341 tgcttataga cggacgaatg acacgttaga ggagaccgtc attcacctct tccgaaaagg
     5401 tattaccatg tcggaaatcg cagacttgat tgagaaaatg tatgggcatt actacacgcc
     5461 ccaaaccatg tccaatataa caaaatcatt tacagaagag gtaacggcgt ttaaagggcg
     5521 ggagcttcat gaccgttatg ctgctattta tatggacgca acgtatattc cgttaaagcg
     5581 gaaaaccgtc gccaaggaag ctattcatat cgcagttggc attcgcccgg acggatcaaa
     5641 ggaagtattg agctatgcga ttgcaccgac tgaatccatc acgatttggg aggaaatttt
     5701 attggacctt caagagcgcg gtttgaaaaa tgtcctcctg ttcatcacgg atggcttaaa
     5761 ggggatggta ggagcgatta gtcggttcta tcccaaagct cgttttcaac attgttgtgt
     5821 acacgtttcc cgtaatatca gtcacaaagt gcgtgtcgat gatcgtaagg aagtctgtga
     5881 tgattttaaa atggtgtatc aagcctcatc taaagaggtg gcgttggaag cacgtggtgc
     5941 ttttgcggaa aaatggaaaa ccagctatcc aaaagtggtt gaatcgattc tttcgaacga
     6001 tcacttgctc actttctatg atttcccctt ggccatacgc aagagtattt attctacgaa
     6061 cttgattgaa tcctttaata agcaaatcaa gaaatacagc caccgcaagg aacagttcca
     6121 aaacgaagag tcgatggaac gtttcttagt ctcgtctttt gatacttaca accaaaaatt
     6181 cctaggtcgc agtcataaag gctttcaaca ggccgaaggc gaacttgaac aaatgctaag
     6241 ccaactgatt gagaattaga aacaatctgc agtagggaag aatcattta
//
LOCUS       DAUVUY010000094         6008 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.094, whole genome shotgun sequence.
ACCESSION   DAUVUY010000094 DAUVUY010000000
VERSION     DAUVUY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.094"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..668
                     /locus_tag="VOX58_001662"
     CDS             <1..668
                     /locus_tag="VOX58_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HES5878875.1"
                     /translation="FAKDQLSQKLTQAQNERIAYLENYNQSIEQLYREIRTVKHDSEN
                     ILISLKDSIDSGDIDLITRVYDTVIQQSATSMMRTNYEISSLDNIKEAVIRSIMNSKL
                     LEAQYLGIELYIEIPDVIDHLPIKLIDLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQ
                     DNKQLFIIENSTKTNRVDIAKRFDAQQQNSAHFLTVLDSYPQITLSTKSDHYRLRQLL
                     EMR"
     gene            672..1412
                     /locus_tag="VOX58_001663"
     CDS             672..1412
                     /locus_tag="VOX58_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5878876.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            1952..2311
                     /gene="rnpA"
                     /locus_tag="VOX58_001664"
     CDS             1952..2311
                     /gene="rnpA"
                     /locus_tag="VOX58_001664"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HES5878877.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            2277..3104
                     /locus_tag="VOX58_001665"
     CDS             2277..3104
                     /locus_tag="VOX58_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HES5878878.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            3116..4030
                     /locus_tag="VOX58_001666"
     CDS             3116..4030
                     /locus_tag="VOX58_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HES5878879.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPAVNSADVEPEEIKATQKLEAED
                     TKVVPLMSEDSPAQTLSNLAETVTETKAQQPSIPVEESEVPQDPGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            4345..4479
                     /gene="rpmH"
                     /locus_tag="VOX58_001667"
     CDS             4345..4479
                     /gene="rpmH"
                     /locus_tag="VOX58_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HES5878880.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            4753..5457
                     /locus_tag="VOX58_001668"
     CDS             4753..5457
                     /locus_tag="VOX58_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HES5878881.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMAEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            5506..>6008
                     /locus_tag="VOX58_001669"
     CDS             5506..>6008
                     /locus_tag="VOX58_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HES5878882.1"
                     /translation="MNMKKLASLVMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTEEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALN"
BASE COUNT         1958 a         1045 c         1251 g         1754 t
ORIGIN      
        1 gatttgctaa agatcagctg tcacaaaaat taacacaagc acaaaatgaa cgtattgcgt
       61 atcttgaaaa ttacaatcaa tcgattgagc aactttaccg agaaatcaga acagttaaac
      121 atgattctga aaatatttta attagtctaa aagatagtat cgatagtggt gatatagatc
      181 tgattactag agtttatgac acggttattc agcaatcggc aacttctatg atgcgcacaa
      241 attatgaaat atcttcttta gacaatatca aagaggctgt gattcgcagt atcatgaatt
      301 ccaaattgct tgaagctcag tatttgggta ttgaacttta tattgagatt ccagatgtta
      361 tcgatcattt acctattaag ttaatcgact taattgtact ttttactgga ctagtggaca
      421 atgctattga gacagccaaa ggcagtagac gtcctttttt atctattgca tactttaaac
      481 aagataacaa gcaattattt attattgaaa atagcacaaa aaccaatcgt gtggatatag
      541 ccaaacgctt tgatgctcag cagcagaact cagcccactt tttgactgtt ttagatagtt
      601 acccccaaat aacgctttcg actaaaagtg accattatcg attaagacaa ttgttagaaa
      661 tgagataaag tatgatgaac atttttattc ttgaggatga ttttatccag cagacacgca
      721 tagaatctat cgtagtaggc atcttaaaag agactagaat accatgtaat cagcttgaag
      781 tgttctcgac tcctcaaaaa ttatttgaga gtatccaaga aagaggagac catcagctgt
      841 attttttaga tattgaaatt ggtgaatata ctcgttgtgg gcttgaatta gctgcagcta
      901 ttcgacaaaa agatcctaat gccgttattg tgtttgtcac cactcattca gaatttgctc
      961 ctatttcttt taaatataag gtatccgctc tagattttat tgataaagca ggtggtcaaa
     1021 aacagtttaa agaacaaatc gaagaatgta tccgatatac ttacgatatg atgtctagtc
     1081 gtgagtcaaa ggacatgttt ttatttgaga ctccacagac aagattaaaa ttgccctata
     1141 aggatatttt atactttgct acagccacaa ctcctcacaa agtttgttta tggactcaga
     1201 cagaaagatt agagttttat ggtaatctat ctgagattca agcagtagca ccgaaacttt
     1261 ttttgtgcca tagatcctac cttgtgaatc tagataaggt tgttcgaatt gataagagta
     1321 agcagctcct ttatttcgaa aatggtgatt cttgtatggt atcacgatta aaaatgaaat
     1381 gtctgtttga acggtgggag gctcttcgct aaagttaaga gagcaataac attttaggac
     1441 gaaaaagcaa catttcagga aatgatgctc ttattgaaac atttttcgct ataataatta
     1501 gtgtaaataa agatttacga agtcatgagt ttatcgagca atcaatcccc aattcaatca
     1561 aaatattgtt ttctctctct ccataaacaa atagtatttt tgatatgatg gctcggcaga
     1621 caaaatgacg atgtcagttg tctttgtttg gatatcacat tagctgattg taaaatcagt
     1681 atcaaagccc ggcttgccgg gttttttgat aggtaaatac agggataagt agctgacgtc
     1741 ttgtggattc tgacttttta tggttgtttt ctgaaggtta ttcctttgtc gaaagaagaa
     1801 ctatactacg aaaagatgga gtgacaagag gcagttgaag cacaaaaact agaaatagta
     1861 cagaaagagg cttaatctga aagggttagg gctttttaat tttttagggg atgtggtata
     1921 atggtaataa tttagatatt ggagttaaat tttgaagaag acctatcgtg tcaagcgtga
     1981 gaaagatttc caagccatat ttaaggatgg aaaaagtaca gcaaatcgaa aatttgtcat
     2041 ttatcattta aatagaggcc aagaccactt ccgtgtgggt atttctgtcg gtaaaaaaat
     2101 aggaaatgca gtcaccagaa atgcagtcaa acgaaagata cgtcatgtta tcatggcatt
     2161 agggcatcag ctgaagtcag aggatttcgt ggtgattgcc cgtaagggtg tcgagtcttt
     2221 ggagtatcaa gagcttcaac aaaatttaca tcatgtttta aagttagcac aattgcttga
     2281 gaaaggtttt gagagtgaag aaaaacatta aaattgcaag aatagtcccc ttagttctat
     2341 tattagtggc ctgtggtcgt ggtgaggtaa cggcacaatc atctagtggt tgggaccagt
     2401 tggtttactt atttgccaga gcaattcaat ggctttcctt tgatggttca attggtgttg
     2461 gcattattct ttttaccctt actatccgtc tcatgctcat gcctttgttt aatatgcaaa
     2521 tcaaatcaag ccagaagatg caagacatcc aacctgagct tagagaattg caaaagaaat
     2581 acgctggtaa agacacacaa acgcggatga agttggctga agaaagtcaa gccctttaca
     2641 aaaaatatgg ggttaaccct tatgctagcc tcttacccct cttaattcag atgccagtta
     2701 tgattgccct attccaagcc ttgacacggg tatctttctt aaaaacaggg actttcttgt
     2761 gggtggaatt ggcacaacat gatcatttgt accttttacc agttttggca gctgtcttca
     2821 ctttcttgtc cacttggttg accaatctag cggctaaaga aaaaaatgtc atgatgactg
     2881 ttatgattta tgtgatgccg ctaatgatct ttttcatggg ctttaacttg gctagtggag
     2941 tagtgctcta ttggacggtc tcaaacgcct ttcaagtggt acaactactg ctattaaaca
     3001 atccttttaa gattattgcc gaaagacagc gtcttgccaa tgaagaaaaa gaacgccgtc
     3061 ttcgtgaacg tcgtgctcgt aaaaaagcaa tgaagagaaa ataggagttt aggttatggt
     3121 attatttaca ggaaaaacag tcgaagaagc tatcgaaaca ggacttcaag agttagggtt
     3181 atcacgcctt aaagcgcata tcaaagtcat ttcaaaagag aaaaaaggtt tcctcgggtt
     3241 cggtaaaaag ccagcccaag ttgatatcga aggtatcagc gacaagacgg tttataaggc
     3301 tgataaaaaa gccactcgag gtgttccaga ggatattaat caaaaaaatg ctcccgcggt
     3361 caattctgct gatgtagaac ctgaagaaat aaaagccact caaaagttag aagcagaaga
     3421 taccaaagtg gttcctctca tgtctgaaga tagtccagcg caaacccttt ctaatcttgc
     3481 tgagacagtc actgaaacaa aagcacaaca accgtcaatc ccagtcgagg aatcagaagt
     3541 gccccaagat cctggtaatg atggtttcag caaagatatt gaaaaagctg ctcaggaagt
     3601 gtccgattat gtgactaaaa tcatctatga aatggatatc gaagccaccg ttgaaaccag
     3661 caacaatcgt cgccaaatca atttgcagat tgaaacgcca gaggcgggac gtgtgattgg
     3721 ttaccatggt aaggttttga aatccttgca gttgttagct caaaatttct tgcacgaccg
     3781 ctattcaaaa aatttttcag tatccttgaa tgtccatgat tatgtggaac atcgaacaga
     3841 aaccttgatt gactttacgc aaaaagttgc caaacgcgtc ttggaatctg gtcaagatta
     3901 caccatggat cccatgagta acagtgagcg taaaatcgtt cataaaaccg tttcatccat
     3961 tgaaggggtt gatagttatt ctgaaggcaa tgaccctaac cgctatgtgg tagtcagtct
     4021 tcaacgttag atcctaaaaa gaatctttgg tagttagttt agctatcgaa gattttttgt
     4081 atggccagct taaccgtttg ttttactgat ctcattgatt attgttgcta ttgataaggt
     4141 ttcaacggta aggcaaagga acagtgtcaa agaagagaca ctttatgaaa tgagcggcct
     4201 cttaaatgag agagaaactg ttgaaaaacg aaaataaatc ttgacagtag accctcttta
     4261 agatagaata ttatagtatg tttagtaact gatatcacaa atagccggct aaacgaatac
     4321 aaaatctatg aggaggtatt cactgtgaaa cgtacttatc aaccaagtaa aatccgtcgt
     4381 caacgtaaac acggattccg tcaccgtatg tcaactaaaa acggacgtcg cgtgcttgct
     4441 gcacgtcgtc gtaaaggacg taaagtatta tcagcttaat tgcaaaaaat acaactaaag
     4501 taaaccaaga gtgctcgagg ctcttggttt tttaacgttt tctaatcggg aatttgcaat
     4561 gtataagtat ggtgtcaact ccttaccttt ttatttgttg tatgaaagag cattccaaat
     4621 aatagaaaat aatataaaaa atgaaagtag attctaaaag tctcattgct attgaaagcg
     4681 ctttattttt tgttataata actttgtgaa cgttattttt attaaaacta agaaacacaa
     4741 ggagtagaaa ctatgcctga taaaccaacc aaagaaaagc tcatggagca gttaaagggt
     4801 gggattatcg tttcttgtca ggctttgcca ggtgagcctc tctattcaga gactgggggt
     4861 attatgccac tgctggcaaa agctgctcaa gaagctggtg ctgtcggcat cagagctaat
     4921 tcggttagag acattaaaga aattcaagcc ataacagacc taccaattat cggtatcatc
     4981 aaaaaagatt acccgccgca agagcctttt attacggcaa cgatggccga agtggatcaa
     5041 ttagctgcct taaatattgc agtgattgct atggattgta ccaagcgtga ccgacatgat
     5101 ggcttggata ttgctagctt tattcgacaa gttaaggaaa agtaccccaa tcagcttttg
     5161 atggcagata ttagtacctt tgatgaagga ctagtggccc atcaggctgg cattgatttt
     5221 gttggaacca ccttgtcagg ctacaccccg tatagccgtc aggaagcagg tcctgatgtg
     5281 gcattgatag aagctctttg caaagcaggc attgctgtca ttgccgaagg aaagattcat
     5341 tccccagaag aagccaagaa aattaatgac ttaggtgtgg caggtattgt tgttggaggg
     5401 gccattacaa ggcctaagga aattgcagag cgttttatcg aagcgctcaa atcctaatag
     5461 tggttgtcaa aatagaaaca cacatcaaaa aaaggagatt ttacgatgaa catgaagaag
     5521 ttagcttcat tagtgatgct tggtgcatct gttttaggac tagcagcttg tggcggaaag
     5581 agccagaaag aggctggtgc aagtaaatct gatactgcta agacagaaat cacatggtgg
     5641 gctttcccgg tcttcacgca agaaaaagct gaagatggtg tgggaaccta tgagaagaaa
     5701 ttgattgctg cctttgagaa agctaatcca gaaatcaagg taaaattgga aaccattgat
     5761 tttacatctg gtcctgagaa aatcacaact gctattgaag ctggaacagc tcctgatgtc
     5821 ctctttgatg ctccaggtcg tatcattcaa tatggtaaaa atgggaaatt ggctgacttg
     5881 aatgacttgt tcacagaaga gtttaccaaa gatgttaaca atgacaaatt gattcaagcc
     5941 tcaaaagcag gagatacagc ttacatgtat cctatcagct ctgctccatt ctacatggcg
     6001 ttgaataa
//
LOCUS       DAUVUY010000095         5965 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.095, whole genome shotgun sequence.
ACCESSION   DAUVUY010000095 DAUVUY010000000
VERSION     DAUVUY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.095"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..917
                     /locus_tag="VOX58_001670"
     CDS             <1..917
                     /locus_tag="VOX58_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HES5878883.1"
                     /translation="GLLYYLYKGTSLGIYPPLIFLCLGASTDFGPLIANPKTILLGGA
                     AQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLAPDLLSTIALAAYSY
                     MALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVTIFVSLLVPSATTLV
                     GCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANGDLFLSATTLKIIAL
                     GLIAFAAGTAGGVLMGKVMYYLSGNKVNPMIGAAGVSAVPMAARVVQKVGQEEDPSNF
                     LLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(1118..2002)
                     /gene="citG"
                     /locus_tag="VOX58_001671"
     CDS             complement(1118..2002)
                     /gene="citG"
                     /locus_tag="VOX58_001671"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HES5878884.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGCFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(1995..2690)
                     /locus_tag="VOX58_001672"
     CDS             complement(1995..2690)
                     /locus_tag="VOX58_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HES5878885.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKTTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(2848..4251)
                     /locus_tag="VOX58_001673"
     CDS             complement(2848..4251)
                     /locus_tag="VOX58_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HES5878886.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
     gene            4631..4954
                     /locus_tag="VOX58_001674"
     CDS             4631..4954
                     /locus_tag="VOX58_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878887.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSAIAAGDRPNSQFKVKRILKRNPEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
     gene            5011..5406
                     /locus_tag="VOX58_001675"
     CDS             5011..5406
                     /locus_tag="VOX58_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HES5878888.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVPVPTEA
                     SPQVEEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VASSAGTITAIHVGPGQVVNPGDGLITIG"
     gene            5451..>5965
                     /locus_tag="VOX58_001676"
     CDS             5451..>5965
                     /locus_tag="VOX58_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HES5878889.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIGTELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMA"
BASE COUNT         1889 a         1182 c         1182 g         1712 t
ORIGIN      
        1 gtggccttct ttattaccta tataagggaa ctagcctagg gatttaccca cctttaattt
       61 ttctttgttt gggggcatca actgactttg gtcccttgat tgccaatcct aaaaccattt
      121 tactaggagg cgcagcacaa gttgggattt tcttagcctt tttccttgct attatgttag
      181 gcatgacacc tcaagaagcg gcttctgtcg ggattattgg tggtgccgat ggaccaacag
      241 ctatttatgt gacgacaaag ttggcgcctg atttactctc tactatcgcc ctagcagctt
      301 attcttacat ggctctggta cctattattc aacctcctat cattaaactc ttgactacca
      361 aggcggaacg ccaagtgaaa atgactcaag caagaactgt ttcacaaaaa gaaaaaatca
      421 ttttcccgat tatggtaaca atctttgtca gccttttggt tccaagtgct acaaccttgg
      481 ttggctgttt gatgctggga aatctggtca gagaaattaa aattgttcct aaaattgtag
      541 agaatctcca acaagtggtt atgttttgca ttactatcat tttaggatta accgttggtg
      601 ctaaggccaa tggagatctc tttttatcag ccacaacctt gaaaattatt gcccttgggc
      661 taatcgcctt tgctgctggt acagcagggg gtgtcttgat ggggaaggtc atgtactatt
      721 tatctggtaa caaagtgaac cctatgatag gagcagcagg tgtttcagct gttccgatgg
      781 cagcgcgagt ggtccaaaag gtcggacaag aagaagatcc aagtaatttc ttactcatgc
      841 acgccatggg acccaatgta gctggcgtta ttggctcagc cattgcttca ggtgccttgc
      901 tggccttctt tggctaattg tgaaaaagga ttagtaaatt aatcttaata aatcattatc
      961 aggagttgga agcaatctag ctcctttttt agtagaaagc tcgagtgata aactattcca
     1021 aaataattat aaaaaaagac tgtcctgatt gtacggacta tatcaattga cttttatagt
     1081 ctagctgaaa tagcagtgca aaactaagac actcgtttta aagtagtttt tctaaaaaag
     1141 caaaataaaa ggtcagagcc aataaatctg ccgcgccccc agggcttaaa tgctgattaa
     1201 tgaggcattg attgtaggag gacaaagcca atcttaggtc agttgttgat aaatcttgtt
     1261 gcaggagtag tcgcatatct gctttaacgc ctttccatgc ttcaatccct ccacgatgaa
     1321 tcagatttcc atcttcaaca atagacatca aataaacaag aagacgaagc tgactggttt
     1381 cggggtcatt ttgacttatc atctgacgaa agtagggcaa ggcatggtct gttaaggtcg
     1441 tgaatccctc gcttgcctca ccccgaggtc ctttgatgcc ataagttaca aagagttgct
     1501 ccccataagt gaactctttt ttagtgttca aatgaccaag gtctgtttga atcaaatgtg
     1561 ccgtcatagg ttttactaaa cgacagatag ctagagtatc ttccttgcta aaacacccta
     1621 aatcagtcat taagtgaggt gttcttgcta gataggcccc agttgcacca agaagtaaag
     1681 ccatggaaaa attcaggcct ttatgagtgt taattccctg agtagcacaa aacatggttt
     1741 cctcggcctt ttgacctagt tgtctaagac ggttaaacag aagaagtggc tcttcttttg
     1801 cataagcaaa acctgtttcg atataagcct gaaaaaaagg agagagtgct atcatactgt
     1861 cgataaaggt cataaaggac atgtcatcat gggcaccgtt atcaaatcga tcaactaaac
     1921 caggttttgg agatagtgag acttcataaa gcaaagcttt taaggccagt tgactaatac
     1981 ttgttagaac tgccttagtc attttgctca ggcctcattt cttttaagat aaattcaagt
     2041 gagctattca agtgttcatg tgtaatcaac gtaatttgct catgatcttt gttcttcata
     2101 cctcggtaaa tcatccagtg ttcagataga gctcgtttac ggcgttcaac tgaagaaatc
     2161 gaaatttttc taaagtagac caaataagct tgcaattctg tcacaatttc ttttaagcgg
     2221 accatttggc tatagctgta aatcaaattg ttaaaggcat taaaattatc caaaatacga
     2281 ttggtgtcat cctctgcgat aaaggtttca cagtcttcca acaagttatg catgacttta
     2341 aaatcttctt ctgtcattaa atgcatcgct tgcactgttg cgagagtctc tagagctttt
     2401 cgaatttcaa aaatttcaca ggcatccttg atactaactc ctttgacaat aatgcctttt
     2461 ttaggaatgt gctctacaag gtgttcttca gacaataatc ccaaagcgta gcgaataggt
     2521 gtcctgctaa tattaagcgc aatggaaaac tctttttcat taatgcggct tccaacagga
     2581 atttgtcgca aaataatggt cgttttaaga gcgttataaa aggctatttt caaagggata
     2641 tttcttgata ggtcaagatt ttgtttaacg gcttcgataa taggattcat gtcatctcct
     2701 taatgctagt gtcctcactt tattatactt aaatttgtga aacattccaa taggactaga
     2761 tgagttctga aaaattaaag ccatgctcac aaaaggacat gactttaatc atctcataag
     2821 gaataaggta gtagtgttat cgttggttta ttgtggaatg taaagtggca tttgccccat
     2881 ggcaataatg gttaccacaa agataacaaa gatgataagg gcatatttag cagcctcttt
     2941 ttgccattcc cccatgtcaa gacctgtcaa gcgaagaaga agataaatga aggcaactaa
     3001 tggactaagt aagtggaagg cttgtcccat gagggaagca agtgccatag ccatgttact
     3061 gaaaccatat tcagcaccag cttctgcaag aacaggcaag ataccatagt aaaaaccatc
     3121 attggagata aagaaggtac caggtgcaga aactaaggca ataacaagac cccagaaacc
     3181 tgccagttgt tttggaataa tggttgcaaa actttgtgca agagcgctag ccataccaga
     3241 accttggaaa agacccataa agataccagc agcaaagact aagataacca cttgaaccgc
     3301 atcaccagca ttgtcaccaa tgcgttttga ttgatcttta agtactgggt aattgaccat
     3361 caaggcgata caagttccca ccgcaaaaag aagaagcggt gccatagcaa tagatttgtt
     3421 gaaagagcca gcaactaacc aagtgattaa tccaatggtt aggatagcat tgaaaacaaa
     3481 gtttttagga cgacgaataa aaagggtatc tgggtcagta atgtctgtga gttgacgcat
     3541 gtcttcttca gacaagtcaa taacaccaag tcttgcacgt tctttacgcc ccatgcttgg
     3601 agcaacccaa caaatcacat aaagaagaga taaaatcata cccggtgcaa gatagccaag
     3661 aatttcagga ccgacaccaa gaacagacat cgcacgagca gtagggccac cccatggcag
     3721 taagttcata atagtatttt gaagaataat caagacacct aggttcatga ttttcatgtc
     3781 caatttttta tagataggta agaaagcaga gcaacaaatt aaagtagtgg ttgttccatc
     3841 accattaaga gatactgcag cagcaacgac agctgttgcg ataagcacct tcattggatc
     3901 acctttagca aagcgaatca tcttttcagt aatcggatca aacaatcctg catctaacat
     3961 gacagagaag aacaaaatgg caaatagcaa catgatacct gttttagcag tattgttgat
     4021 accatacata accattggtc cgatagctgt taggtcttta gcaattccac cgtcaccaac
     4081 aaaggcaaca aatttggcat ctgcattgaa gtcagctgaa ccagtcagta taacagcaat
     4141 cgtcatgatt aatggaatca tgaccaaagc agtaaaaggg gtcatttttt tcttcattac
     4201 cacgtacata aagacaataa tcatggcata agccagcatt gttaataaca tcattgttat
     4261 ctcctttttc aagttatgat ttcagtatag atgaaagcgc ttataaaata aatacttctt
     4321 tgtgcagaaa gacacaaaaa aaacaatttg aaaattaatt ttgaaaacca tttttattac
     4381 atcagatgac tttaaatttt ctataataat gatgaaccta gttagatcaa gcttttcttg
     4441 ctatcagtat cttgatctca gtttttcaac agttacctaa aaataaaata aaaggtaact
     4501 gttgaaaaac tttatgaaac attgaaagcg ctcttagaaa gaacacaaaa aagccatctc
     4561 aaaaaagatt ggtagtatat aataaactta ctagttccct aaataggtaa atgtgaaaaa
     4621 gaggtagctc atgcagtttt tacaaaaatg gtttaaacac tctaaaaaag agaagttgaa
     4681 agaatcctcg ccccttagta cagaaataga accttctgaa aactgggaaa agattcccgc
     4741 ttatattcct gcagacaaaa gtgattacaa aaaggttact ttaattacca gtgctattgc
     4801 tgctggcgat agacctaata gtcaattcaa ggttaaacgc attctcaagc gcaatcctga
     4861 ggcaatcact gtttctttaa ttgcttcaag tatcgctgca ggtgtttacc cagaaagtca
     4921 gtttcgggtg acatccatct attgcaaaag gtagtcgttg aacgatactt tagacgaact
     4981 catgacacaa taacatatag gagaaaaatg atgttacgaa aatttaaaat taccattgac
     5041 ggtaaagaat atcttgtaga aatggaagaa atcggtgctc ctgcccaagc agctgccccc
     5101 gctcaaccaa tatccactcc agtacctgtc cctactgaag caagcccaca agtggaggaa
     5161 gcgcaagcac cacagccagt agctgcagcg ggagcggatg ccattccatc accaatgcct
     5221 ggaaccatct taaaagtctt agtagcagtg ggagaccaag taactgaaaa tcagccacta
     5281 ttgattttag aagccatgaa gatggaaaat gagattgtgg cctcatcagc aggaaccatt
     5341 acagctatcc acgtcggtcc aggtcaagtg gtcaatcctg gtgatggtct cattacaatt
     5401 ggttaatagt actataacta gaataaatag acagaaaagg aagtgaattt gtggacactt
     5461 taatcgcagg aataacctcc atcaccattc ctcaaattgt tatgatggtc atcggtgccc
     5521 tcttaatgta tctaggaatt aaaaaagaat acgaaccaac cttacttgtc cccatgggac
     5581 ttggaaccat tctcgttaac ttccctggtt caggagtttt aacccaagtg gttaacggag
     5641 tcgagcaaga aggggttttt gaggctctct tcaattttgg gattggaaca gaacttttcc
     5701 cactgttgat tttcattggt attggcgcca tgattgactt tggtcccttg cttcaaaacc
     5761 cattcatgct cttgtttggt gctgcagcgc aatttgggat tttctttgtt gttgttgttg
     5821 ctgtactggc tggctttgac attaaagaag cagcttcaat cggcattatc ggagcagcag
     5881 atggaccgac ttctatcttt gttgccaacc aactggccaa ggatttactg ggtccaatta
     5941 cggttgcagc ctattcttac atggc
//
LOCUS       DAUVUY010000096         5828 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.096, whole genome shotgun sequence.
ACCESSION   DAUVUY010000096 DAUVUY010000000
VERSION     DAUVUY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.096"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..266
                     /locus_tag="VOX58_001677"
     CDS             <1..266
                     /locus_tag="VOX58_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HES5878890.1"
                     /translation="TLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLLNYF
                     DINGNYHQKAVKHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            263..1249
                     /locus_tag="VOX58_001678"
     CDS             263..1249
                     /locus_tag="VOX58_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HES5878891.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQRKIKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDIILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            1228..1749
                     /locus_tag="VOX58_001679"
     CDS             1228..1749
                     /locus_tag="VOX58_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HES5878892.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            1746..2207
                     /locus_tag="VOX58_001680"
     CDS             1746..2207
                     /locus_tag="VOX58_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HES5878893.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            2204..2950
                     /gene="xerD"
                     /locus_tag="VOX58_001681"
     CDS             2204..2950
                     /gene="xerD"
                     /locus_tag="VOX58_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HES5878894.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLIGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEAYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPVTLEKYYKS"
     gene            2950..3654
                     /locus_tag="VOX58_001682"
     CDS             2950..3654
                     /locus_tag="VOX58_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HES5878895.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKTNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            3651..4202
                     /locus_tag="VOX58_001683"
     CDS             3651..4202
                     /locus_tag="VOX58_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HES5878896.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            4324..5046
                     /locus_tag="VOX58_001684"
     CDS             4324..5046
                     /locus_tag="VOX58_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HES5878897.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            5043..5306
                     /gene="yidD"
                     /locus_tag="VOX58_001685"
     CDS             5043..5306
                     /gene="yidD"
                     /locus_tag="VOX58_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HES5878898.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            5484..>5828
                     /locus_tag="VOX58_001686"
     CDS             5484..>5828
                     /locus_tag="VOX58_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HES5878899.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFL"
BASE COUNT         1949 a         1003 c         1180 g         1696 t
ORIGIN      
        1 atacccttgt actaggatgt actcactatc ccttgttacg accaattatc caaaatgtta
       61 tggggccatc tgttaagctg attgacagtg gagcagaatg cgtccgagat atctctgtct
      121 tattaaacta ttttgacatt aacggtaatt atcatcaaaa agctgtcaaa caccgctttt
      181 ttacaacagc aaatcctgag atttttcaag aaattgcctc gatttggctg aaacaaaaaa
      241 taaatgtgga gcatgtcacc ttatgagtga aaaaatttac gaatacaaag atgaaaataa
      301 ctggtttatc ggaaagatga ctggtcataa tttaatatcg ggctggggag tcaaacacac
      361 gacaatcaaa aaaatagatg accttttaga cggtattgcc gcaacgttag attgggaaaa
      421 tcctaaaggt tacgatgttt cggtggtgcg ttatcagtct cctttatctc tcatcacctt
      481 tattatcgac atgataaatc aagagaccca gcgtaagatt aaggtaactc cccatgcagg
      541 cacgatttta ttaatggaaa atgcgaaact gcttgcagtt tatctcccag aaggtggagt
      601 ttcaacggct acattttttg cgactagtga acagggattt ggggatatta ttttaatcgc
      661 tacacgaaac gagggaaaaa ccaaagagtt ccgcaatctg tttggccagc taggttatcg
      721 tgtggaaaat ctcaatgatt atccagaatt gccagaagta gcagaaactg gtactacttt
      781 tgaagaaaat gctcgcttga aagcagaaac gatttcacgc ctcacaggaa aaatggtatt
      841 agcagacgat tcaggactaa aagttgatgc cttgggtggt ttacctgggg tttggtcagc
      901 tcgtttctca ggaccagatg ccacagatgc caagaataat gccaaactct tacacgaatt
      961 agctatggtg tttgaccaaa agaagcgctc agctcaattt catacgactt tggtagtggc
     1021 tgctcccaat aaagatagtt tggttgtgga agccgagtgg ccaggctata ttgctacgca
     1081 gcctaaggga gaaaatggtt ttgggtatga ccctgtcttt atcgtcggag agactgggcg
     1141 tcatgctgca gaattagagg ctgaccaaaa gaatcagttg tcgcatagag gtcaagcagt
     1201 tcgaaaatta atggaggtct ttccagcatg gcaagcaaaa caatcatagt aatgagtgac
     1261 tctcatggtg accgagatat cgttcaagcc attaaagaca agtatcttgg tcaagttgat
     1321 gctattttcc ataatgggga ttctgaatta aacagctcag atcctatttg ggctggtatt
     1381 tatgtcgttg gcggtaattg tgactatgat acaggttacc ctgatcgttt agtaactcaa
     1441 ttaggcacag ttacgattgc ccagacacat ggtcacttgt accatattaa tttcacttgg
     1501 gataaactgg actactttgc tcaagaagta gcagcagata tttgtctata tggacaccta
     1561 caccgtccag ctgcttggca agttgggcaa accctcttta tgaatcccgg atcagtaaca
     1621 caacctcgcg gagaaattaa cgagaaatta tatgctcgtg ttgaactaac tgatactcaa
     1681 attaaagttg attactttac aagagatcat aaactttacc catcactgtc taaggaattt
     1741 aaacgatgat cgcaaaagaa tttgaaacgt ttttaatgag tcatttagat aattatctta
     1801 tcccggaaca agacttggct atttttatcg acacacataa tgcagaccat gtgatgttac
     1861 ttttggtgag caatggtttt tctagagttc ctgttatcac acgggagaaa aaatatgtgg
     1921 gaaccattag catctcagat attatgatgt accaatctaa aaggcagctg acagattggg
     1981 aaatgagtca aacagatatt ggcgagatgg ttaataccaa aattgaaacc attagcatta
     2041 cttccagcct aacagaaatt atgcacaagt taattgattt cccattctta ccggttgtag
     2101 atagagcgaa tcgctttgtt gggattatta cgcgaaaatc tatattaaag gctgtcaaca
     2161 gtcttttaca tgattttaca gatgattaca cgattataaa aaaatgaaga gttatattga
     2221 accttttatt gctagcaagg ctttatctca aaatagtcaa aaagcttatc gatacgattt
     2281 gcaacagttt tgtcaactga ttggtgaacg tgtcaatcaa gacaaactat tactttatca
     2341 aaatagtata gctaacctca gtttatctgc aaaaaaacgc aaattatcaa cagctaatca
     2401 attcttatac tacttgtatc aaataaagta tttaaatagc tattttcgat taacagacac
     2461 catgaaagtg atgcggactg agaagcagca agctgctatt attaatactg atattttcta
     2521 tcaaaaaacc ccttttgtgt gggggcaatt aatcagctta ttaatattgg agctgggttt
     2581 aactccgagt gaagtagctg gtattgaagt agctaacttg gatttaaatt ttcaaatgtt
     2641 gacgctaaag actaaaaaag gtgtgcgtgt attacctttg tcgcaaatac taatcccttt
     2701 tttagaacaa caactcgttg gcaaagaagc ctatcttttt gaacatcgag gcatcccttt
     2761 ttcaagacaa tggtttttta atcacctaaa aacctttgtt cgaagtattg gttatgaagg
     2821 attaactgct caaaaattac gagagcaatt tattttgaaa gaaaaactag ctggtaaatc
     2881 gataatagaa cttagcgata ttcttgggct gaaaagccct gtgaccttag aaaaatacta
     2941 caaatcataa tggatataaa acttaaagat tttgaaggtc ctcttgattt actcttgcac
     3001 ttggtatcac agtacaaagt tgacatctac gaggttccta ttgttgaggt tattgagcag
     3061 taccttaatt atattgaaac cttacaggta atgaaacttg aagtggctgg tgactacatg
     3121 ctgatggcaa gtcagctcat gttaatcaaa agtagaaggc tattaccaaa ggtggtagaa
     3181 cacatcgaag aagaagattt agaacaagat ctgttagaaa agatcgaaga atatagtcgc
     3241 tttaaagcag ttagtcaagc gttagcgaag caacatgacc aaagggcaaa atggtattca
     3301 aaacctaaac aagaattaat ttttgaagat gctatcttac aagaagataa aacggtgatg
     3361 gatctatttc tagctttttc aaacataatg gcagctaaaa gagcagtttt aaaaaccaac
     3421 catacagtca tcgagcgtga tgattataag attgaagaca tgatggcttc tatcaaacag
     3481 cgactggaaa aagaaaacgt tattagctta tcagcgattt ttgaggaatg ccaaacatta
     3541 aatgaagtta tcagtatatt tttagctagt ctagaattga ttaagcttca cgtggtattt
     3601 gtagagcagc tctctaattt tggggctatc atactacgaa aggagaagaa atgacgtatt
     3661 tgtcacagat agaagcgctg ctatttgttg caggtgaaga agggttaagt ttgcgacatc
     3721 tagcgtctat gttatcatta acgccaacag ctcttcaaca gcaattagaa aaactctctc
     3781 aaaagtatga aaaagatcaa cattctagtc tttgtttgat agaaacagct aatacctaca
     3841 ggctagtaac taaagaaggt tttgcagaac tcttgcgtgc ttatgccaaa acaccaatga
     3901 accaaagtct atctcgtgcg agtttagagg tcttgtctat tgttgcatac aaacaaccta
     3961 ttacacgaat agaaattgat gacattcgag gggtaaactc cagtggagct ttgagtaaat
     4021 tattggcatt tgatctcatt agagaagctg gcaaaaaaga tgtggttggg cgtcctcatt
     4081 tgtatgcgac aacagattat tttctggact atatgggaat taatcattta gacgaattga
     4141 ttgaagtatc tgccgttgaa ccagcagatg aagagattgc tctttttagg acacaagact
     4201 gagttttgct tgtcatcaaa tagcaaatgg cgtttacaaa aggatttgtt ttaaggcgtt
     4261 ttagttgaaa aaaagcctta aactagggac aacaaaaagt tgctcaaaag aaagaagaat
     4321 ataatgcgaa ttaacaaata cattgcccat gcgggtattg ccagtcgacg taaggctgaa
     4381 gaattgatta agcaaggctt agtaacctta aatggtcagg ttattacgga tttagcaacc
     4441 actgtaaaat cgggagacgt ggttgaaata gaaggaagtc ctatttacaa cgaggaaaag
     4501 gtttactatt tgcttaataa acctcgtggc gctatctcaa gtgtgtcaga tgacaaaggc
     4561 cgtaaaacgg tactggattt actgcctcag gttaaagaac gcatataccc tgttggacga
     4621 ttggattggg acacatcagg ggtccttatt ttaacgaatg atggtgattt tacagatact
     4681 atgattcatc ctagaaatga aatagacaag gtttatctgg cgcgtgtcaa aggaattgca
     4741 acgaaagaaa atcttcgtcc cttaactcga ggaattgtta ttgatggtaa aaagaccaaa
     4801 ccagctcgct acaatattgt tcgtgtagaa gctgataaga atcgatcgat cgtagaattg
     4861 actatccatg aagggcgaaa ccatcaggtt aaaaaaatgt ttgagtctgt ggggcttttg
     4921 gttgataaat tatctcgtac acgttttggg accgtcgact taaagggact aagaccagga
     4981 gaagcaagac gcttaaataa aaaagaaata agtcaactgc ataacttagc taatacgaaa
     5041 aaatgatgaa aaaactatta atagtatctg ttaaagctta tcaaaaatac atttctcctt
     5101 tatcgcctcc tagttgtcgc tacaagccaa cctgttcagc ttatatgtta actgctattg
     5161 aaaaacatgg tactaaggga atactaatgg ggatagcaag aatattaaga tgtcaccctt
     5221 ttgttgctgg tggtgtcgat cctgttccag aagattttag cctaatgaga aataaaaaca
     5281 cgtctaaaaa cgctgaaaag gcatgacttt atcaaaaaat ggcttgacat tgatagctga
     5341 agtgttataa tggaaaacaa gcattcgttg gtttaaatca aacctgttat gatttaagtt
     5401 agcgagtcac catcaaccac attgtttgct gagcatgact ccgagcagtg tggtttttta
     5461 aattaaaaag agtaaatgag attatgacga caaaagaact tatcaacaaa aacgacaaag
     5521 taaaaaaggc tcgcaatcat attgtcctgt ttcaacccca aattccacaa aatactggta
     5581 atattgctag aacatgtgct gcaactaatg cacctttaca cattattaaa cccatgggat
     5641 ttcctattga tgaccgcaaa atgaagcgtg ccggcttgga ttactgggac aagttagaac
     5701 tgcattttta tgatcattta gaacagttta tcaatcaatg tcacggacag cttcacctga
     5761 ttagcaagtt tgcagttaat aattattctc aagctactta tgctgatggc gattcccatt
     5821 atttcttg
//
LOCUS       DAUVUY010000097         5649 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.097, whole genome shotgun sequence.
ACCESSION   DAUVUY010000097 DAUVUY010000000
VERSION     DAUVUY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.097"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..162)
                     /locus_tag="VOX58_001687"
     CDS             complement(<1..162)
                     /locus_tag="VOX58_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HES5878900.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAIN"
     gene            complement(215..400)
                     /locus_tag="VOX58_001688"
     CDS             complement(215..400)
                     /locus_tag="VOX58_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HES5878901.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            538..1107
                     /locus_tag="VOX58_001689"
     CDS             538..1107
                     /locus_tag="VOX58_001689"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HES5878902.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            1142..2221
                     /gene="prfA"
                     /locus_tag="VOX58_001690"
     CDS             1142..2221
                     /gene="prfA"
                     /locus_tag="VOX58_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HES5878903.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            2221..3060
                     /gene="prmC"
                     /locus_tag="VOX58_001691"
     CDS             2221..3060
                     /gene="prmC"
                     /locus_tag="VOX58_001691"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HES5878904.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLSYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            3044..3634
                     /locus_tag="VOX58_001692"
     CDS             3044..3634
                     /locus_tag="VOX58_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HES5878905.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITTALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            3652..4104
                     /locus_tag="VOX58_001693"
     CDS             3652..4104
                     /locus_tag="VOX58_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HES5878906.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            4094..5350
                     /locus_tag="VOX58_001694"
     CDS             4094..5350
                     /locus_tag="VOX58_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HES5878907.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     HYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIAHLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            5357..>5649
                     /locus_tag="VOX58_001695"
     CDS             5357..>5649
                     /locus_tag="VOX58_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HES5878908.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFV"
BASE COUNT         1755 a          981 c         1173 g         1740 t
ORIGIN      
        1 attaatagcc atcaattcag agtttgaatc attagctgaa aaacgatttt tgtaagtata
       61 taataagtca atgactctac ttagttgttg gcaagctttg tcagattcga tctgaatatc
      121 aataactgtt cccattagct ttaattgttg cgtaacaaac ataattagtc ctctttttag
      181 cagaataccc ccactaagga gtgggggaag gtcattatga tttttgtttc atttcacctt
      241 gaggatagta agttccttca ggcatatcgt tgataaaaac atggatattt tctttaggtg
      301 cttttgcaat gcgtgagact acttcggtaa cctcgcgagc taattgattt ttttgttctt
      361 gtgagcgtcc ctcaaacaag tcaattgtta caaatggcat aatgttactc ctttatgtta
      421 ttgggcatca ttgtaacata atgctgtgaa aaagtataca ctgttttcaa tggctgggaa
      481 aacttcagcc tttgtggtaa aataggtaga atactataga aagtaggatt ttccgctttg
      541 gctcaactct attataaata tgggacaatg aattctggta aaaccattga aattttaaaa
      601 gttgctcata actatgaaga acaaggcaaa cctgttgtca ttatgacaag tgctcttgat
      661 acacgtgatg gttttggcat cgtctcaagc cgtattggta tgcgacgaga agctatcccc
      721 atttcaaatg acatggatat tttcacattt attgcccaat tggaggaaaa accttattgt
      781 gtcctgattg acgaaagcca atttttaagc aagcaaaatg tttatgattt ggcgcgtgtg
      841 gtggatgagc taaatgttcc tgtcatggct tttggtttga aaaatgattt ccaaaacaat
      901 ctttttgaag gttcaaaaca tttgctatta ttggccgata agattgatga aatcaaaaca
      961 atttgccaat actgtagtaa gaaagcaacc atggttttaa ggacagaaaa cggaaaacct
     1021 gtctatgaag gtgatcagat acaaattggt ggtaatgaaa catatatccc agtttgtcga
     1081 aaacattatt ttaatccaga gatttaaaag gtaatcggtt ttaaaggaga tacaaagaaa
     1141 aatgaatatt tatgatcaat tgcaggcagt tgaagatcgt tatgaagagt taggtgagtt
     1201 acttagtgat ccagatgttg tttcagatac aaagcgattt atggaattat cacgtgaaga
     1261 agctaatact cgagaaactg tcacagctta tagagaatat aagcaagtca ttcaaaccat
     1321 ttcagatgct gaagaaatga tcaaagatgc cagtggtgat cctgaattag aagaaatggc
     1381 taaagaagaa cttaaagaat caaaagcagc taaagaagag tatgaagaaa aattaaaaat
     1441 ccttctatta cctaaagatc ctaacgatga taaaaacatt attttagaaa ttcgtggtgc
     1501 tgctggtggc gatgaagcag cgctttttgc tggtgatctt ttaacaatgt atcaaaaata
     1561 tgctgaaact caaggatggc gttttgaagt tatggaatca tccgttaatg gtgttggtgg
     1621 tattaaagaa gtggttgcca tggtttctgg tcagtcagtt tattctaagc ttaaatatga
     1681 atcaggagct catcgtgttc aacgtgtccc agtgacagag agtcagggtc gtgttcatac
     1741 ttcaactgca actgttttgg taatgccaga agttgaagaa gtagaatacg acattgatcc
     1801 taaagacctt cgcgtcgata tttaccacgc gtctggtgct ggtggtcaga atgtcaataa
     1861 agttgctact gcagttcgta tggtacacat tcctacagga atcaaagttg aaatgcaaga
     1921 agagcgtact cagcaaaaaa accgtgataa agcgatgaaa atcattcgtg ctcgtgttgc
     1981 tgaccatttt gcgcaaattg ctcaagatga acaagatgct gaacgtaaat caacagttgg
     2041 tactggtgat cgttcagaac gtattcgtac ttataatttc ccacaaaatc gtgtaacaga
     2101 tcatcgtatt ggtttgacac ttcaaaaatt agatactatt ttatctggaa aaatggatga
     2161 agttattgat gcactcgtta tgtatgatca gacaaagaaa ttagaatctc tcaataacta
     2221 atgaactatg ctacattaat cagaacttat gaagataaac tagaacaaat agatgaggat
     2281 cgcgaaaatc tctcctatgt ttttagagaa atcaaagaat ggtcaagtct agatatgttg
     2341 attcaccaaa atcaagctgt gactcctgaa gatgctgttc ttttggaaca tattttctgt
     2401 tctctaagtc agcatttatc tcctcaatat attacaggta atgcttattt tcgtgatttg
     2461 aagcttgcag tcgacaagcg tgtattaatt ccaaggccag aaacagaaga attagttgac
     2521 atgattttag ctgaaaatct tgatgcaccg ttgaatgttt tagatattgg tacaggtagt
     2581 ggagcgattg ctatttctct caaaaaagaa aggcctaact ggcaagtaac ggcatctgat
     2641 atttctagag ctgctcttga cttggcaaaa gccaatgctg atgcttacca gcttgatata
     2701 acgtttattg aatcagatgt ttttagtctc atttcaggaa catttgatat tattgtgtca
     2761 aatcctccct acatatccta cgaggataag gaagaagtca gtctaaatgt tttgcaatca
     2821 gagcctcatt tggcactttt tgctaaagaa aatggctatg ctatttaccg taagattatt
     2881 gaacaagctg acaattactt aactaaggag gggaagcttt actttgaaat tggttataag
     2941 caagctgaag gtataaaaga tatgttacaa gcctattttc ctcaaagaca tatacgagtc
     3001 gtaacggata tatttggcaa agaaagaatg gtggttgttg attatggagt atctagctag
     3061 tatcattgaa tcaggtgatg cccttgtttt gccaacagaa acggtttatg gtctttttgc
     3121 caaggcttta gatgaaaaag cagtaaatgc tgtttatgac ttaaaacaaa gaccccgaga
     3181 taaagcaatg aaccttaatg tggctgattt taatagtatc ttagcctttt caaaagaaca
     3241 gccgagatac ctcaagaaat tataccaagc ctttttacca ggtccgttaa cgattatttt
     3301 aaaagctaat gaccaggttc cttattggat taattcgggt ctttcaacgg ttggttttcg
     3361 cttacccagt catccaatta ccacagcttt aattcaaaaa acaggccctt taattggtcc
     3421 ttctgcaaac ttatctggca aagctagtgg tcgagttttt gatcatatta tgcaagattt
     3481 tgactttcaa gtgtttggtt atgcagatga tccttttcta acaggtaagg attcaaccat
     3541 cttagactta tcaggtgagc gtgctgtgat tttacgtcaa ggtgctatca ccaaagaaga
     3601 attattagcc aacgtgcctg aacttcgctt ttaagagagg agccgcttgg aatgttgaga
     3661 ccattaagta aaacggactg tcctatgcta caagaaatta acgccaaagc ccttggctat
     3721 ttagtttcgc ttgacttgtt agaaaggcaa tatgaacgat tgattgagga ttgccatcac
     3781 tattttttag cttatgccga caaggatacc aatcaattat taggctatgt tcacgctgaa
     3841 cggtatgaga ccctttatgc ttctgatggt cttaatctct tgggcttggc tgttttacca
     3901 gcctatcaga gaaggggaat tgggagtgcc ttactaaggg ccctggaatc ccaagctagg
     3961 caggaaggca ttgcctttat tcgcctcaat tctgccagtc atcgtaaaga agcgcatgct
     4021 ttttaccgaa acctcgacta tgcagatgat aaaacacaat tacgattcat caaaaactta
     4081 caaggagatg acaatgattt ttgataaagg taatgttgaa gactttgata aagagctttg
     4141 ggatgccatt catgctgaag aagaaagaca agaacatcat atcgaattga ttgcatctga
     4201 aaacatggtt tcaaaagctg tcatggcagc tcaaggttct gttttaacca ataagtatgc
     4261 agaaggctat ccaggtaatc gctattatgg tggaacagaa tgtgtggata ttgtcgaaac
     4321 gcttgctatt gaacgtgcta agaaactttt cggtgccgca tttgccaatg ttcaagccca
     4381 ttccggaagt caagccaatg cagcagctta tatggccttg attgaggctg gtgacaccgt
     4441 attaggaatg gatttggctg caggtggtca tctcacgcat ggctctccag taaatttttc
     4501 tggaaaaacc taccattttg tgggttactc agtagatgct gatactgaaa tgttaaatta
     4561 tgaggcgatt ttagaacaag caaaggcggt ccagccaaag cttatcgttg caggggcttc
     4621 ggcctactct agaagcattg attttgaaaa gtttcgtgcc attgctgatc acgtaggtgc
     4681 ttacttaatg gtggacatgg ctcacatcgc tggccttgta gcagcaggtg tgcacccgag
     4741 tcctgtccat tatgcgcata ttgtgacatc aacaacccat aaaaccttac gtggtcctcg
     4801 tggaggcttg atattaacca atgatgaagc tcttgctaaa aagattaatt cggccgtttt
     4861 ccctggttta caaggaggac ctctggaaca cgtgattgct gccaaagcgg ttgccttcaa
     4921 agaagccttg gatccagcct ttaaagacta tgcacaagct attattgata atactgcggc
     4981 tatggcagca gtttttgcac aagatgatcg tttccgtctg atctcaggtg gtacagataa
     5041 tcatgtcttt ttagtagatg tcactaaggt tattgctaat ggtaaactag cgcaaaatct
     5101 tctggatgag gttaatatta ctctaaataa aaatgccatt ccttttgaaa ccttatctcc
     5161 ttttaaaaca tcaggcattc gtattggttg tgcagctata accagtcgtg gcatgggagt
     5221 taaagaaagc caaactatcg ctcacctcat catcaaagcc ttggttaatc atgatcaaga
     5281 aactattctt gaagaggttc gtcaggaagt gcgtcagtta accgatgcct tcccactcta
     5341 taaaaagtaa ggaatcatgt tagatagtta tataaaacgt attgtcattc atcaattttc
     5401 tcccaatgac actgagttac tgttatcaga tcgtttagtg agcattacac cacgaattga
     5461 tgaatatttc cggaaaaaat tggctaaagt gttctcggat gaagccaaac ggggtcagtt
     5521 tgaggctaat aatacctttt ttacaaccat tggtgatgat ttgttggaaa cgtctgttac
     5581 cattgcccag ttatggaagg aagcctttgt tatttcagag gatcaaaaaa ccaatgattt
     5641 agtctttgt
//
LOCUS       DAUVUY010000098         5516 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.098, whole genome shotgun sequence.
ACCESSION   DAUVUY010000098 DAUVUY010000000
VERSION     DAUVUY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.098"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..136)
                     /locus_tag="VOX58_001696"
     CDS             complement(<1..136)
                     /locus_tag="VOX58_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878909.1"
                     /translation="MWSFWKRYRPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     K"
     gene            complement(138..1130)
                     /locus_tag="VOX58_001697"
     CDS             complement(138..1130)
                     /locus_tag="VOX58_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HES5878910.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNEVLDLNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEKTTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQSFGKMKSLSFELKPGQEQVVSQFMGLPDIT
                     VERHELSLDIQYDSSRYQTADIIQKTMADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            complement(1271..3214)
                     /gene="thrS"
                     /locus_tag="VOX58_001698"
     CDS             complement(1271..3214)
                     /gene="thrS"
                     /locus_tag="VOX58_001698"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HES5878911.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTRKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSCPDAQA"
     gene            complement(3636..4970)
                     /locus_tag="VOX58_001699"
     CDS             complement(3636..4970)
                     /locus_tag="VOX58_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HES5878912.1"
                     /translation="MRVGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(4972..>5516)
                     /locus_tag="VOX58_001700"
     CDS             complement(4972..>5516)
                     /locus_tag="VOX58_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HES5878913.1"
                     /translation="RYFDIKEGEKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGE
                     TNKWVIPAQVRQMVNGNHPKNLIFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLE
                     ALASRQHLVLRDIPVYYGWVDQSSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQS
                     RRLETVGHALVDVYKKVMEL"
BASE COUNT         1630 a         1229 c         1022 g         1635 t
ORIGIN      
        1 ccttccagag gtaataagcc acaaaagccc ccatgacatc tccaatacga tacaaaaaga
       61 aattaacgcg ataggttatt aattcctgaa ttcctgcact gagaaagggt ctataacgtt
      121 tccaaaatga ccacatctta aagctccttt cggtagaaac gacgaacaat atcctcaata
      181 tctacatcag tcatcttcaa atccctgacc gcaaaatcag ccatggtttt ttgaataatg
      241 tccgctgttt gatagcgcga actgtcatat tgaatgtcta aactaagctc atgccgttcc
      301 actgtgatgt caggtaagcc cataaactgt gagaccacct gctcctgccc tggcttcaac
      361 tcaaaagaca gactcttcat ttttccaaag ctttgcttta attgggtcac cgttccatca
      421 aagatttctt gccctttatc aatcatgata atccgatcac agagctgttc aatatcactc
      481 aaatcatggg tcgtcagtaa aatagttgtc ttttcttctt gattaatttg agtaatggca
      541 cgccgaatgt tgtccttcac agaaacatca agtccaatcg ttggttcatc taaaaacaat
      601 actttaggat tatgcaacaa ggaagcagca atatcagctc gcatccgctg accgagtgat
      661 aaggtccgga caggatcctt aataaactca ttcaggtcca aaacctcatt taaaaaatcc
      721 atgcgcttgc gaaaggcttt ttcaggcaca tcgtaaattt cttttaaaac gacataggtc
      781 tcttgcaagg ccaaatccca ccatagttgt gttcgttgac caaaaacagc tccaatgtct
      841 ctcacataat cttgacgatt atcttgtgga atctttccgt taatgcgaca gtaacctgat
      901 gtaggcttta aaatacctgt taacatctta atcgttgttg attttcctgc accattggct
      961 ccaataaaac ctaaaatttg ccctttgggt acctcaaaag ataagtcctt aaccgcctca
     1021 aagatttctc tttggggatg aacaaaggac ttcaaggctc cctttaatcc tggctcttta
     1081 atggttttgg aaaaattttt ttgaaggtgg gacacttcta tcatcaccat ttaccattcc
     1141 tcgtttctgt tatcgtctta tcaccgttac taaacaattt attataacac catgaaaaag
     1201 actgtgcaac cgtttgctta attttttaag aaagaaaaag aagcccataa ggacctcttt
     1261 ttgccattga ttaagcttgt gcatctggac atgacttgcg agcaatgtct gccaagatgt
     1321 tttccacaaa ttcttccaca gattcagtgt gagtcgcctt actaccatag cggcgaacat
     1381 tgactgattt gtcttccatt tctttgtccc caacaattaa ttggtaagga attttgctgg
     1441 tttgagacgc acgaatcttg tactgcattt tttcattgcg gtcatcgaca tctgcacgaa
     1501 caccacggtc acgaagggtt tttgctactt cccaagcata gtcaatgtgg gcttcatttg
     1561 aaattggaat cacggttact tggtgaggtg ccaaccaagt tgggaaagca cccttgtaag
     1621 tttcaataag gatagcagtg aaacgttcca tagttgagat aacaccacgg tgaatcatga
     1681 ctgggcggtg ttcttcgcca tctgcaccaa tgtatttaag gtcaaagcgt tctggcaaca
     1741 agaagtccaa ctgaatggtt gatagggttt cttcgttacc aagagctgtt ttcacctgaa
     1801 tatcaagctt aggcccataa aaggctgctt caccttctgc ttcaaagtaa tctacaccca
     1861 tctcatcaag tgcagcttta agcatacttt gagcattttc ccacatctca tcattgtcat
     1921 aatatttacg agtatcgtta gggtctcggt atgacaaacg aaaacgataa tcagtcaagt
     1981 tgaagtcagc ataaacatca atgattaatt gaagggcacg ttgaaattct tcctggattt
     2041 gctctggtgt tacgaaaatg tggccatcat tcaaggtcat ttcacgtacc cgttgaagtc
     2101 ctgatagggc acctgatttt tcgtaacggt gcatcatgcc aagctcggca atacgaatag
     2161 gcaattcacg ataagaacgc acatgatttt tatacacttg aatgtggtgt gggcagttca
     2221 ttggacgaag aacaaactcc tcaccatctc ccatatccat tacagggaac atatcttctt
     2281 gataatgatc ccaatgacct gatgtcttgt acaattctac agaagcaagt ggtggtgtgt
     2341 aaacgtgttg gtagcctgat gctaattctt tatctgtgat gtagcgctca agtgtacgac
     2401 gaatagtcgc tccatctggt aaccaaaacg gcaagccttg accaacttct tggctaatca
     2461 tgaacaagtc aagctcttta ccaagcttac ggtgatcacg ttctttggct tcttctagac
     2521 gagtcaagta agctttgagg tcttttttat caaaccaagc tgttccatag atacgttgca
     2581 tcatgttatt gtcgctgtta ccacgccagt aagcacccgc cacatttagc aagtggaaga
     2641 cttggatacg gcctgttgat ggcacatgag ggccacggca aagatccaca aattcacctt
     2701 gacggtaaac cgtcaagcca gcaccagcat gctcattaat caattctacc ttgtaaggat
     2761 catctttgaa caattccaaa gcttcttctt tagtcacttc ttcacggata catgggtaat
     2821 tttctgtcac gattttttgc atttcagctt cgatacgtgg caaatcttcg tttgaaattt
     2881 ggccttcagc attatctgta tcatagtaaa agccttctgc aatggcaggg cccacaccca
     2941 aatgaagatt tgggaacaag cgttttgcag cttgggcaaa taaatgggct gcagagtggc
     3001 gaagcacctc ataagcatct ttgtggtcag gtgtcacgat ttcaatactg ccatcttctt
     3061 caatggcacg agtggtgtca atgagttgat cattgaactt accagctaaa gcttttttag
     3121 ccaatgattt gctgatggac tcagcaatgt caaacgttgt tacaccagat tcaaattcac
     3181 gcacggcgcc atctggaaaa gtaatcttaa tcatttgctt tctcctttaa gtcagttaag
     3241 atttccacta aaacatggat agtaagacaa attagggaca agaggttagt gaatagctca
     3301 ataccaccac gtagaagaac aataccttat tcacgaatct accctcccta gtccttaaaa
     3361 acaaggctaa tcccctccaa aacaaaaaaa ctgaccgcat cccaaaggac gcaatcagcg
     3421 tggttccacc ttaatttatg cagggcaaaa aacatgcctt gcacctctag tggcgataag
     3481 ggggccaccc ttttatgttt gcataaaaac caggaaggag tatcagatta gctatccttg
     3541 tgctctttcc accaaccagc actcgcttaa aagattctgt ccaatctaac ttgtcttcaa
     3601 gataattata gcaagttttc aaaaatttac aagtcttaat ccagaaaatc tttgatagca
     3661 tggaccagct tgataggagc cttgactact tttactgagg tgatagctgt gaccttagca
     3721 gcacgtttag gcaagtgaat agcaccttgc actaatttta gagaactact tttaccagaa
     3781 tgattaagat aaagactcag cttttgagct tctttgctat ttctagcaat taacgtatct
     3841 aaatagaacg tgtaaataga ttttccaaag tgctgtgctg agatttcata acgttttttt
     3901 gctaataacc gtttatccat aactggtgtt tttagtatgg catcaataat agcatcagtt
     3961 aaatctgttt cagcgtaata aagagtgcca aacattttat cagtcaccac atcatctaaa
     4021 taaggattgc catgagcaat aataggagtg ccactagcca aactttcaat ataggtcaag
     4081 ccctgagtct cactggtcga tgctgagata aagaaatcac aagccttata gtacagagca
     4141 accttatcat gaggcaccat gcctgtaaag gtcacgtgtt tgtcaacctc taactgcatc
     4201 gccaagtgtt tcaaatcttg caaatagggg ccatctccta caataataag ctttatcttg
     4261 gcattttcag ccaaaatagc tggcatctga ttgatgatag cttgaatatt tttttcataa
     4321 gaaatccgtg acaaactcaa taacatggtt tcatcaccag caatgcccaa ttctgctttt
     4381 aagttggtta cttcttctgc tgtgatgtca tcacgaatat atttttccaa aggaatgcct
     4441 gttgggataa cccgcttagg gatagtaact tcgtaacctt ctagaagatt gaggacaatc
     4501 ctacttgggc agataacccc atccaaatcc ttcaaatagc ccctaagaag aggtttgacc
     4561 atacttggtc gaatgatttt tccgttggca atataactca cgtagtcctc atattgggta
     4621 tggtaagtat ggacaacagg aattcgcaaa gctttgccta tcattttccc taacaagcct
     4681 aagctaaact cagtttgcgt atgaataata tcaagattat agtgttttgc aattttgtat
     4741 gacgaaatga ggccacgata aaccacacgt ctatccgtaa atgacacaaa aggaacactt
     4801 ggcagtcgaa taatggtcgg gtcttcaaag cgtttgacat ctctatcagt agtggtgaaa
     4861 atataaactt cgtgaccttc tttttctagc tcttctttta acgtacgaat actagtagcg
     4921 actcctgaaa cttgtggaaa ataggtatct gtgaatagac ctacacgcat cttataactc
     4981 cattactttt ttatagacat ctactaaggc atggccaacc gtttctaggc gacgactctg
     5041 ggcaaccttg taaccagctt caactttgtt gctggcacca gaaaagactt ttttcagagc
     5101 ttctataaaa cctggtatat cggttgctaa ttccgcacta ctttgatcaa cccatccgta
     5161 gtaaactggt atatcacgta aaacaaggtg ctgacgactg gccaaggctt ctaagacaac
     5221 aatgccttct gtttcttcac gacttggaaa gaaaaaggca tctgcaccag tcatggcacc
     5281 ttcataaaca tcccctttaa tgtatcctgg gaaaataaga tttttcgggt ggttaccatt
     5341 gaccatttgg cgaacttgag caggaatgac ccatttgttg gtctcgccaa accagataaa
     5401 acgaacatct ggcatagctt gggcaacttt gacaaagtca tcaattcctt tcctcagaaa
     5461 aaataatcct gctcccataa ccactttttc accctcttta atgtcaaagt agcgac
//
LOCUS       DAUVUY010000099         5300 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.099, whole genome shotgun sequence.
ACCESSION   DAUVUY010000099 DAUVUY010000000
VERSION     DAUVUY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.099"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1189
                     /locus_tag="VOX58_001701"
     CDS             <1..1189
                     /locus_tag="VOX58_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HES5878914.1"
                     /translation="NEDSQRIVLAEKKKNTSDLVIKAVKHIKKDQKDYYYFSPIKQAD
                     DFFVDNLPVSLYKKKNSDKELILVKPKLQSSHLRSVNTLTISKIVYQKKFFHLAKKSE
                     KVISTYHVTDDLKPFQVRDLVSGHLERIQEEVEKKYPNAGFNSDKYNGLKESNSLLSD
                     GFEVKSGNLIFDKKLTIPLTTLFDVINPDFLANSDRAAYDNYRTYKEQHPKKLVALTF
                     DDGPDPTTTPQVLDILAKYQAKGTFFMIGSKVVNNENLTKRVSDAGHEIANHTWDHPN
                     LTNLSVSEIQHQVNMTNQAIEKACGKKPRYLRPPYGATNATVQQSSGLTQMLWTVDTR
                     DWENHSTDGIMTNVKNQLQPGGVVLMHDIHQTTINALPAVMEYLKAEGYECVTVSELY
                     AHQ"
     gene            complement(1299..2384)
                     /locus_tag="VOX58_001702"
     CDS             complement(1299..2384)
                     /locus_tag="VOX58_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HES5878915.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2482..3108
                     /gene="udk"
                     /locus_tag="VOX58_001703"
     CDS             2482..3108
                     /gene="udk"
                     /locus_tag="VOX58_001703"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HES5878916.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            3188..3451
                     /locus_tag="VOX58_001704"
     CDS             3188..3451
                     /locus_tag="VOX58_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HES5878917.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERNLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3504..4313)
                     /locus_tag="VOX58_001705"
     CDS             complement(3504..4313)
                     /locus_tag="VOX58_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HES5878918.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     AEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4458..4955
                     /locus_tag="VOX58_001706"
     CDS             4458..4955
                     /locus_tag="VOX58_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HES5878919.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLEAFVDLFLEKTTFTFNM
                     FGVKN"
     gene            4955..>5300
                     /locus_tag="VOX58_001707"
     CDS             4955..>5300
                     /locus_tag="VOX58_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HES5878920.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPS"
BASE COUNT         1763 a          847 c         1059 g         1631 t
ORIGIN      
        1 aaacgaagat agtcagcgta tagttttggc tgaaaagaaa aaaaacacgt cagatttagt
       61 gatcaaagct gtaaaacata ttaaaaaaga tcaaaaagac tattattatt tttccccgat
      121 aaaacaagca gatgattttt ttgtagataa tttacctgtt tcattataca aaaaaaagaa
      181 ttcagataaa gaattgattt tagtaaagcc taaactgcaa tcttctcacc taagatcagt
      241 taacactttg actatttcta aaatagttta tcagaaaaaa ttttttcatt tggctaaaaa
      301 atcagaaaaa gttataagta catatcacgt tacagacgac ttgaaaccgt ttcaggtaag
      361 ggatctagta tcaggacatt tagaaagaat acaagaagaa gttgaaaaaa aatatccaaa
      421 tgctggtttt aatagcgata agtacaatgg cttaaaagaa tctaattctt tattaagcga
      481 tggctttgag gtaaaatcgg gaaaccttat ttttgataaa aagctaacga tacctttgac
      541 gacattattt gatgttatta atccagattt tttagcaaat agcgatagag ctgcgtatga
      601 taattatagg acctacaaag aacagcatcc caaaaaacta gttgcattaa cgtttgatga
      661 tggtccagat ccgacgacga ctcctcaagt tttagatatt ttggcaaaat accaggctaa
      721 gggaactttc tttatgatag gttcaaaagt tgtgaataat gaaaacctta ctaaacgtgt
      781 tagcgacgct ggccatgaaa ttgctaatca tacttgggat catcccaatc tgactaatct
      841 ttcagtcagc gagattcaac atcaagttaa tatgacgaac caggctattg aaaaagcttg
      901 tggcaagaaa cctcgctatt tacgccctcc atacggagct acaaatgcaa ctgttcagca
      961 atcctcagga ttaacacaaa tgctttggac ggttgataca agagattggg aaaatcatag
     1021 cactgatggc attatgacta atgtcaaaaa ccaattgcaa ccgggaggag ttgttttaat
     1081 gcatgatatt catcaaacaa ctatcaatgc tttgccggca gttatggaat atctaaaagc
     1141 agaaggatac gaatgtgtga ctgtatcaga actctatgcg catcagtaag taatatcgtt
     1201 atgtttcttt agaattgata tactaaacaa tccaattact ttgaagtaat tggattgttg
     1261 tttttagtag cccttgatta agttttgttt tttaacaact agttgatgaa atgaagttgt
     1321 tgatttttga gggagatttc agagacttta gcaaattttt tcaatttttt gaggtcttca
     1381 gggtggctaa caaaggtaat aacgatacca gattttccca tacggccggt tcgtccagct
     1441 ctgtgagtat aattttcctt atcacgagca acatcaaaat gaatgacata gtcaagatta
     1501 tcaatatcaa tgccccgagc aacaagatcc gtagctaaca ataaggagag ttggtgggat
     1561 ttgaattttt cgagaatggt tttacggaat ttgacattga tgtcactcgc taaggaaacc
     1621 gcagcagctc cattatattg gagacgttct tccgtagcac ccaaatcaga taaactgttg
     1681 aaaaagacta aagctctaaa atgaggaata ttagcaaatt ttcgtaataa atcggtacgt
     1741 tcgcgcttgt caaccatgag gtaaaaatgc tgaatagcat cgttggtttg ctctgaaaga
     1801 tcgatgcaga atgtatttgg agctaaggaa gtttgatcca ccttattagt agcactcatg
     1861 tataccattt gatgatctcg aggaacatag tggctaattt tttgaacaaa atcatattgg
     1921 gaatcaccta atagttcgtc atattcatct aaaacaatgg tgttgacaga catcatcttg
     1981 atttttttca atttaatcag ttcaaaaatg cgtccagggg tcccaatcag gatttcagga
     2041 ccttttttga ggcgttcgat ttgacgtttt tgactggttc cagaaataaa gagttgtgcc
     2101 gttaagccta gtggctgtgc ccaatccttt gtgacttcaa aaatttggcc agctaattcg
     2161 gtattagggg ctaaaatgag tagttgttgg gactttttag gcgtcagtgc caatagacta
     2221 ggaaagacat aagctaaggt cttgcccgtc ccagtagggc taataccaag gaaattttta
     2281 ccatcaacaa taggttgaaa ggcctgttct tggattggcg ttaaatgggt aaatgcaact
     2341 tggtcaagtt tttcttgcca ttgaggggga aatttagtga tcatgcgtct tccttttact
     2401 attgtttcta tattcattat agcattatag tctcaatttt ggtaaaatag tgagaaagac
     2461 tttttagatt tggagtttaa aatgcttaaa aaaccgatta ttattggtgt aactggtggt
     2521 tcaggtggcg gtaaaacaag tgtgtcacgc gctattttgg acagttttcc aaatgctcgt
     2581 attgcgatga ttcaacacga ttcttactat aaagatcagt ctcacatgag ttttgaagaa
     2641 agggtcaaaa ccaattatga ccatcctcta gcttttgata cagattttat gattcagcaa
     2701 ctaaaagaat tattagctgg tagacctgta gatattccta tctatgatta caaaaagcat
     2761 acccgcagta atacgacctt tcgtcaggat cctcaggatg ttattattgt tgaaggtatt
     2821 cttgtcctag aagatgagcg tttgcgagat ttgatggata tcaagctctt tgtagacaca
     2881 gatgacgata ttcgtattat tcgtcgtatt aagcgtgaca tgatggaacg tggcagaagc
     2941 ttagaaagta ttattgatca gtacacaagc gtggtaaagc ctatgtatca tcagtttatt
     3001 gagccaagta aacgctatgc tgatattgtt attccagagg gagttagtaa tgtcgttgct
     3061 attgatgtta ttaatagtaa aattgcgagt attcttggag aagtatagtg gaagtggctg
     3121 agtagattat ttaaatctgc tcttttattg ttgtgctctt catagagaaa gggatcataa
     3181 aatagaaatg ttattaattt ggcttggatt actatttgtt tggaatgaac tcgtgtttgt
     3241 catatatgcc tttgataaaa gaaaagccat aaaaaagaaa cggcgtatta gtgaaagaaa
     3301 cttattagtg ataacagttt tgtttggagg ttttggagca ttacttgcag ctaaaaagta
     3361 ccaccataaa accagaaagt ggtactttgt catcacttgg tatactagta ttttgttgac
     3421 attactagta acttatatct ttatgaagtg agtcacttaa aagaccctta atcagtatta
     3481 tttagtcaag gctcttttat agtttaaaaa agcacatcat cgtcaagggt gttgattttt
     3541 tgaaaaagat caccaaagat taaaaggatt aatgttccta gtaaaactgt catgccagga
     3601 atgcttgtta gaggagagtt ccagatgaaa tgtagcacca gaggacttaa taaccagaaa
     3661 atttgttgct gttttgaaaa aaggttagaa ccctttaata ataagtatgc tccaatggta
     3721 aaaatagctg tatgtaccca atgtgaagtc acagcagtgc taatacgttc aatagcagtc
     3781 ggtacgagac tatctataga ttttgcttga atcaaataac ggatatcttc tgctagttga
     3841 aagcccatgc cagaaagtaa agcaacaact aatttttctt taagagtaag tctaggaaat
     3901 aaagtaatta ctaaaatagc cgaagaagcc ttaatgggct cttcgacaat aggggcagtt
     3961 aaagcatctc cccaaagctt taaagcgtct ttagaaggga tgatgtatga ccaaaattgt
     4021 cctacgagat ggttcccaca agaagctaaa aaacctgaaa tataaagtcc gctaagacag
     4081 ctaacaataa taacattcag gttcatatga aattttttac ttaaataaaa aatagctgct
     4141 gcaaatggaa ttaagtaaat gcctaataga ctaatggtta gtattagagc aaaagcttct
     4201 ttaaaggaaa ggttgttagc tgtcagtcct aacaattcaa gttcaatacc atttaaaact
     4261 aaagcaataa aaagaccgta tcgtaaccat ttctgttgag tgatttgttt cacgaaaacc
     4321 tcctaatatt aagatgatat taataatatt gaaaattata tcataaacaa cctcaaaaac
     4381 cattttaaaa attgactgaa gcgaaagata gtcgtaaaaa atactcgaat agacccagat
     4441 atgctataat aaaaggtatg aataagtcta aaaaaattga gcaataccaa ctaatgatag
     4501 cacaggcaaa agagctgttt gcgaatgaat caaatgcttt ggctaatttg tcaaatgcca
     4561 gcgccctgtt aaacatgaca ctacccaatt ctgtttttgc gggattttac ctttttgatg
     4621 ggcaggaatt aattcttggc ccttttcaag gtagggtttc ttgcgtccat atcaagcttg
     4681 gtaaaggtgt ttgtggcgaa tcagctcagt ctcgtcgaac cattattata aatgatgtga
     4741 agcagcatgc caactatatt tcctgtgatg cggcagccat gagtgaaatt gttgtgccta
     4801 tggtgaaaga aggccatttg attggggtgc tggacttgga ttctagcctt gtagctgatt
     4861 atgatgaggt tgatcaagaa tacttagagg cctttgtgga cctatttctt gaaaagacca
     4921 cgtttacctt taacatgttt ggagttaaaa actgatgtat caagctcttt atcggaaata
     4981 ccggagccaa acgtttgacg aaatggtggg acaatcggtt atttccacaa ctttaaagca
     5041 ggcagttgaa tctggcaaga ttagccatgc ttatcttttt tcaggtccta gagggactgg
     5101 gaaaacaagt gcggcaaaga tttttgcaaa ggccatgaat tgtcctaacc aagtcgatgg
     5161 tgaaccctgt aatcaatgcg atatttgccg agatatcacg aatggaagct tggaagatgt
     5221 gattgaaatt gatgctgcct cgaataatgg tgttgatgaa attcgtgaca ttcgagacaa
     5281 atcaacctat gcgccaagtc
//
LOCUS       DAUVUY010000100         5205 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.100, whole genome shotgun sequence.
ACCESSION   DAUVUY010000100 DAUVUY010000000
VERSION     DAUVUY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.100"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..504
                     /locus_tag="VOX58_001708"
     CDS             <1..504
                     /locus_tag="VOX58_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HES5878921.1"
                     /translation="KRPSVCNAAEGLVIHEAVAARFIPMLEKAINQVQPVEWRADDKA
                     LPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQHTSHHSEAIITRDIKAA
                     ETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHARGPMGLEALTSTKFYIN
                     GDGHIRE"
     gene            524..745
                     /locus_tag="VOX58_001709"
     CDS             524..745
                     /locus_tag="VOX58_001709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878922.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFDLFGDGM
                     LVSWIKRRKMSKVNQLLIDLQVTLQNCRR"
     gene            788..952
                     /locus_tag="VOX58_001710"
     CDS             788..952
                     /locus_tag="VOX58_001710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878923.1"
                     /translation="MSWDKIFDIWLDNLLTDCCVIKEAKNFLQVVEILEAAAKSVLAK
                     LADSEQKEQD"
     gene            976..1989
                     /gene="rsmH"
                     /locus_tag="VOX58_001711"
     CDS             976..1989
                     /gene="rsmH"
                     /locus_tag="VOX58_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HES5878924.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            1994..2317
                     /gene="ftsL"
                     /locus_tag="VOX58_001712"
     CDS             1994..2317
                     /gene="ftsL"
                     /locus_tag="VOX58_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HES5878925.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            2321..4576
                     /locus_tag="VOX58_001713"
     CDS             2321..4576
                     /locus_tag="VOX58_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HES5878926.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            4578..>5205
                     /locus_tag="VOX58_001714"
     CDS             4578..>5205
                     /locus_tag="VOX58_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HES5878927.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLVGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGAM"
BASE COUNT         1677 a          883 c         1089 g         1556 t
ORIGIN      
        1 aagcgcccaa gtgtttgcaa tgcagctgag ggattggtaa ttcacgaagc tgtagcagca
       61 aggtttattc ctatgcttga aaaagcgatc aatcaggttc agccagtaga atggcgggca
      121 gatgacaagg ctttgccgtt atttgagcag gcagtgcctg caaaagcaga ggattttgag
      181 accgaatttt tagattatat catgtctgtt aaggttgttt cctctttgga agaagctatt
      241 tcttggataa atcagcatac tagtcaccat tccgaagcta tcatcacaag ggatattaag
      301 gcagcagaga cctttcaaga tttagtggat gctgctgctg tttatgtcaa tgcttcaaca
      361 cgatttaccg atggttttgt ttttgggctt ggtgctgaaa ttgggatttc tactcaaaaa
      421 atgcacgccc gtgggccaat gggcttagaa gccttgacca gcactaaatt ttatattaat
      481 ggggatggac atatcagaga ataagctact agaaggtccc ttaatgacta atgaaagaga
      541 aaaagaagaa gcaaaggttg ctttagaaga acttttacat cagattcagg tgattcattt
      601 ggaattgaaa attgtccgaa attggggcta ttttgatctt tttggagacg gtatgcttgt
      661 cagctggatc aaacgtcgaa aaatgagcaa ggtcaaccaa ttattaatag acttacaagt
      721 tactcttcaa aactgccgaa gatagctggc agacgtcgat aggctattat tagctgactt
      781 accgaatatg agttgggata aaatctttga tatttggtta gacaacctgc tgacagattg
      841 ttgtgtgatt aaagaagcta aaaatttttt acaagtagtt gaaattctag aggcagcggc
      901 taagtcagtg cttgctaagt tggctgatag cgaacaaaaa gaacaagatt agagaagagg
      961 aagattccca cgagaatgaa atccttttct cttacttttt catttctaaa ccttttgaag
     1021 tatggtacaa ttaaagttat gacaaaagaa tttcatcacg tgaccgtact ccttcacgaa
     1081 acagtggaca tgcttgacat aaagcctgat gggatttatg ttgatgcgac gctaggtggc
     1141 tcaggccact cagcttattt gttgtccaaa cttggtgaag aagggcacct ctattgtttt
     1201 gaccaagacc aaaaggctat tgacaatgca caagttaccc tcaaatctta tattgacaaa
     1261 ggacaggtaa cttttattaa agataatttt agacacctca aagcacgttt aacagcgctt
     1321 ggagttgatg aaattgatgg tatcttatac gaccttggtg tttccagccc gcaattggat
     1381 gaaagagaac gagggttttc ttataaacaa gatgctccat tggatatgcg catggatcgt
     1441 cagtcgctct taacagctta cgaagtggtg aatacctatc cattcaatga tttggttaag
     1501 attttcttca aatatggtga agataaattc tccaagcaga ttgctcgaaa aattgaacaa
     1561 gcaagagcta ttaagcctat tgagacaaca acagagttgg cagaattgat taaggcagca
     1621 aagccagcta aagagttgaa gaaaaaaggc caccctgcta aacagatttt tcaagctatt
     1681 cgcattgaag tcaatgatga attgggagcg gccgatgaat ctattcagga cgctatggaa
     1741 ttattagccc ttgatggtcg tatctcagtc attaccttcc attctctgga agatcgccta
     1801 accaagcagt tgtttaaaga agctagtacg gtggatgtgc caaaagggct tcctctaatt
     1861 cctgaagata tgaaacctaa gtttgaactt gtttcacgta agccgatctt acctagtcat
     1921 tcagagttaa cagctaataa aagggcacac tcagccaagc tacgtgttgc caaaaaaatt
     1981 cggaaataag cctatgacaa atgaaaaaag aactcaggca gttacaaatg ccttgcaaaa
     2041 acgaataaaa actttctcta gaattgagaa ggctttttat actgctatca ttgtaacagc
     2101 gattacgatg gcagtaagta ttatttacct gcaaagtcgt aagttgcagt tacaacaaga
     2161 aatcactagt ttaaatagtc atatttcgga tcaaaaacta gagcttaata atgccaagca
     2221 agaagtaaat gagttatctc gccgtgatcg tattatagat attgctggta aagcaggctt
     2281 aagcaaccgt aataataata tcaagaaagt cgagtaatct atgaaaaaat ggcaaaaata
     2341 tgttttagat tatgttgtgc gcgataggag aactccagtc gaaaatcgcg ttcgagttgg
     2401 acaaaatatg atgctcttaa ctatctttat tttctttatt ttcattatta atttcatgat
     2461 tattattgga acagatcaaa agtttggagt tagtttgtca gaaggggcga agaaggttta
     2521 tcaagaaacc gttacgatcc aagctaagcg tgggaccatt tatgatcgaa atggtacagc
     2581 tattgcagtg gattctacga cttatagcat atacgcaatt ttggataaat catttgtctc
     2641 ggcttcagat gaaaagttat atgtacaacc ttctcagtat gaaacagtag ctgatatttt
     2701 aaaaaagcat ctgggaatga aaaaaacaga tgtaattaaa cagcttaagc gtaaaggact
     2761 tttccaagtc tcgtttggac cgtcagggtc tggcatttca tatagtacta tgtctactat
     2821 tcaaaaggct atggaagatg ccaaaatcaa gggaattgct ttcacaacta gtcctggtcg
     2881 tatgtatcca aacgggacat ttgcttcaga atttataggc ttggcatctc taacagaaga
     2941 taaaaagaca ggtgttaaga gtttagttgg aaaaacaggt ctagaagctt cttttgataa
     3001 aattttatca ggtcaagatg gtgttattac ttatcaaaaa gatcgaaatg ggaccacact
     3061 cttgggtaca ggtaagactg tcaagaaagc tattgatggc aaagatattt acacaacgct
     3121 atctgagcct atccagacct tcttagaaac ccagatggat gtttttcaag ccaaatcaaa
     3181 tggtcagttg gccagtgcaa cacttgttaa tgctaaaact ggtgaaattt tggcaacaac
     3241 acaacgccct acttataatg ctgatactct gaaaggactt gaaaatacga actacaaatg
     3301 gtacagtgca cttcatcaag gaaattttga gccaggctca accatgaaag tgatgactct
     3361 ggcagcggct attgatgata aagttttcaa cccaaacgaa acctttagca atgctaatgg
     3421 tttaacaatt gcagatgcta ctattcaaga ctggtctatt aatgaaggca tttccacagg
     3481 acagtacatg aattatgcac aaggatttgc cttctcaagt aacgttggga tgactaaact
     3541 tgaacaaaaa atgggtaatg caaaatggat gaattatttg acgaagttcc gctttggttt
     3601 tcctactcgt tttggtttaa aagatgaaga cgcaggtata tttccttctg ataatatcgt
     3661 gactcaagct atgagcgctt ttggtcaagg gatttctgta acccagattc aaatgcttag
     3721 agcctttact gctatttcta ataatggtga gatgttagag ccacaattta tcagtcaaat
     3781 ttatgatcct aacacagcaa gttttagaac ggcaaataaa gaaattgttg gaaaacctgt
     3841 atcaaaaaaa gccgctagtg aaacaagaca atacatgatt ggtgtaggaa cagaccctga
     3901 gtttggaaca ctctattcaa aaacatttgg accaattatt aaagtgggtg atttacctgt
     3961 tgctgttaaa tcaggaacag cacaaattgg ttcagaagat ggaagtggtt atcaagatgg
     4021 tggattgact aactatgtct attcggttgt ggcaatggtg ccagctgata aaccagactt
     4081 tttgatgtat gttactatga ctaaaccaca acattttggt cccctttttt ggcaagatgt
     4141 ggttaaccca gtattggaag aagcatactt aatgcaagat acactaacta agccagtagt
     4201 atcagatgct aatcgtcaaa caacttataa attaccaaac tttgtaggaa agaatcctgg
     4261 tgagacatca agcgagttgc gtcgaaatct tgtccagcca gttgtccttg gtactggcag
     4321 caagatcaaa aaagtatcgc atcagcccgg tcaaacgtta acagaaaacc aacaagttct
     4381 catattatca gaccgttttg tggaggtacc agacatgtat ggctggacaa aatccaatgt
     4441 taaaaccttt gctaaatgga ctggaataga catcagcttt aaaggaacag attctggtcg
     4501 tgttatgaaa caaagtgttg atgttggtaa gtccttgaaa aaaataaaaa aaatgaccat
     4561 tactttagga gattaacatg tttttaaccc ttattgcagc aatcatatcc tttatggtat
     4621 ctgctttcac aatgccttat tttatcaaat tctatcaatt gaaaaaaatt ggtgggcaac
     4681 aaatgcatga agatgtcaag caacatttag ctaaagctgg aacaccgact atggggggaa
     4741 ctgtttttct tttagtagct acggcagtat cgttattggt tagtctattt tcgatcaaaa
     4801 acactcaaag tttagctttg atttcaggca ttttatcaat tgttgtcatt tacgggatta
     4861 tcggattttt ggatgatttt ttaaaaattt ttaaacaaat caatgaagga ttgacagcca
     4921 aacaaaagtt agccttgcag ttggttggag gattgatgtt ctatttttta catgttagtc
     4981 caagtggcat ttcctctatt aatgtgtttg gttatcagct gccgctggga attttctacc
     5041 tattctttgt tttgttttgg gtggttggtt tttcaaatgc cgttaatttg acagatggta
     5101 tcgatggctt agcttctatc tcagtggtga ttagtctagt gacttacggc gttattgcct
     5161 acgttcagag tcaatttgat gttttgttac tgattggagc aatga
//
LOCUS       DAUVUY010000101         5092 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.101, whole genome shotgun sequence.
ACCESSION   DAUVUY010000101 DAUVUY010000000
VERSION     DAUVUY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.101"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..391)
                     /locus_tag="VOX58_001715"
     CDS             complement(<1..391)
                     /locus_tag="VOX58_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HES5878928.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLLLSAAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKRIFSGQQVDIDPLGAIVGIVSAFV"
     gene            complement(511..1833)
                     /locus_tag="VOX58_001716"
     CDS             complement(511..1833)
                     /locus_tag="VOX58_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HES5878929.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNESVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(1961..2047)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2362..4680
                     /gene="pcrA"
                     /locus_tag="VOX58_001717"
     CDS             2362..4680
                     /gene="pcrA"
                     /locus_tag="VOX58_001717"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HES5878930.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALSLNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT         1703 a          983 c         1026 g         1380 t
ORIGIN      
        1 taacaaaagc tgaaacgata ccgacaatag ccccaagagg gtcaatatca acttgctgac
       61 cactaaagat acgtttaatt gtgtgaatca gtacttggaa acctacaaga aacataataa
      121 aagaagtgac aaggctggat aaatcttcaa ttttccaatg accaaattta tgattggcat
      181 cggctggttg gctagctaag tgaagaccaa taagcagggc tacatttccc acaatatccg
      241 ataaattgtt aaatccatca gcaataaggg aacttgcatt taacaaataa ccagctaata
      301 atttggcagc actaagcaac aagtagacaa taatactaac gattgggccc tttctggcta
      361 gttttaaatt tgcaatcgga tcctgtgtca taataactcc tttgatttca actttatatt
      421 ataccataaa aaggggctta agaaaaaagc ctagcttctt gaacttggca gaagaagact
      481 aggtataact gggtagtact aacgtcatgt ttacttaata aagtaatgct tggtttctaa
      541 aataacaact ggcgagagag ctaggagggc aatcaggtta ggaagtgcca ttaaaccatt
      601 gacaatatca gccaaaaccc aaatcagttc taacttaagg aagcctccta ggcctaccat
      661 taagataaag acaatacgga agtaggtgag atgtgtaatg ccaaagagga attcaaagca
      721 gcgttctcca taataactcc atcctaaaat cgttgtaaag gcgaagagaa ctagggaaaa
      781 ggttaggcca aaagtcccta aatttccaaa cacagtggca aaggcagatt gggtcagagg
      841 agccccttcg agctgcccag tccattggcc tgttactaaa atagataggc cggttagggt
      901 acagataata atcgtatcaa taaaagttcc tgtcatggag atcaggccct gctccacgga
      961 ttcattggtt ttagctgctg ctgcagcaat aggtgcagat cctagtccag actcattgga
     1021 gaagacaccg cgtgcaattc ctttttgaat ggcatccttc atgagacttc cagcaaagcc
     1081 tccaatagca gctgtcggag tgaaggctga ttgaaagact aaacgaataa caggaagtag
     1141 ctgctggtag tgactaaaaa taactgctaa actagataga atataaaaaa tagccataaa
     1201 aggaacgact ttttcagcca ctttggaaat ggaatgaatg ccaccaaaaa taatggcagc
     1261 cacaaagata gctaaaacaa tactgaccat ttgtggggat aagccaaagc tatgacctaa
     1321 agacgaggtg atcgagttca cttgagcaaa ggttcctatt ccaaataggg caactaggat
     1381 ccctgaaccc gcaaaaagaa tggctaaggg tttccatttt gtccccatgc cattaacaat
     1441 atagtacata ggaccaccag aaatatgacc gttggcatcc tttgtacggt atttaatcgc
     1501 tagtacccct tcagcgtact tagtggccat tccaaaaaaa gcggcaaccc acatccaaaa
     1561 gagcgctcca ggaccaccag acttgatagc agtggcaacc ccaacaatgt taccagttcc
     1621 gacagtagcg gcaagggcag ttgcaagagc agcaaaggat gaaatatctc catgtccttc
     1681 gtcatctgaa aaaatgagtt taaaggctct tggtagtttt aagatttgaa ttaatcctaa
     1741 acgactggta aggtaaatcc ccgtcccaac caataaaatt aagaggggcg gtccccaaac
     1801 aaggttatca attaatttaa cgagtgctat catctttctc tcctaaaagt catcgaagac
     1861 gccaaaagaa aaaagctatc tctttctttg agacaacttc tgaagcaatg ataaaacagc
     1921 aaacacaaaa aagactaaga ctgttcttaa tggtgtttgt attttacctt ctgtcctttt
     1981 gcctgagaga ttgagcagta tgccttgccc cttcggcgcc taataaggtc tctccagaag
     2041 tccgtccatc aaacagtccc gttcaaaaac tgaacacctg agagtataac tccttcggcg
     2101 aaaaaaattc ttctcctgca tgacatcatt cggtttatta aattaggact atcatacctc
     2161 aaaaaacgaa tgaagtcaac caaaatagca aaaaaataag gaatgcttta agaaatcgct
     2221 attattttta aatatctgat cctaaactag aaaaaaggaa tctctttact tttggggtct
     2281 tctttgctat ttttcttgga aacatgatat aattttaagg ttgataaaag agatgtcact
     2341 gaacgagatt aaggagaaag catgaatcct ttattaaatg gaatgaatga tcggcaggca
     2401 caagctgttc aaacaactga aggcccctta ttgattatgg cgggggctgg ttctggtaaa
     2461 acaagggtac tgacccatcg tattgcctat ttaattgatg aaaaatttgt caatccttgg
     2521 aatattttag ctattacctt tactaataag gctgcgcgtg aaatgaaaga aagagccttg
     2581 tccttaaatc cagctactaa agatacctta attgctacct ttcactccat gtgtgtacgg
     2641 attctgcgtc gtgaggctga tcacattggt tacaaccgaa acttcacaat cgtggatcca
     2701 ggtgagcaac gaacgttgat gaagcgcatt ttaaaacaat taaatataga ccctaaaaag
     2761 tggaatgaac gttctatttt aggaactatt tcaaacgcta aaaatgattt gcttgatgaa
     2821 aagggatacg aggcacaggc tgctgatatg tatagtcaaa tcgtagcgag gtgttacaaa
     2881 gcttatcagg aagaattacg acgtagcgaa gcacttgact ttgatgattt aatcatgatg
     2941 acgctacgcc tgtttgacgc taatcctgac gtgttagctt attaccagca gcgttaccaa
     3001 tacattcatg tggatgagta ccaagatacc aaccatgctc aatatcagct aattaaattg
     3061 ctagcttctc gctttaaaaa tatttgcgtt gttggagacg ctgatcagtc tatctatggt
     3121 tggcggggag ctgacatgca aaatattctt gattttgaaa aagattaccc agatgctaag
     3181 gtagtgcttt tagaagaaaa ctatcgctct accaagaaaa ttctgcaagc agctaacgac
     3241 gtgattaaca ataaccgtaa tcgccgtcct aagaaacttt ggacacaaaa tgcggatggt
     3301 gaacaactgg tttattaccg ggcaaatgat gagcgagatg aggcagtatt tgtggcctca
     3361 accattagca atatgagtca agagcttgga aaaaacttca aagatttcgc tgttttatac
     3421 cgtaccaatg cccaatctcg gaccattgaa gaagcactct taaaatccaa tattccttac
     3481 accatggttg gaggcaccaa gttttacagc cgtaaagaaa ttcgggattt gattgcctat
     3541 ttaaccattg ttgctaaccc tgctgataat atttcttttg agcggattgt gaatgagcct
     3601 aaacgaggtg ttggtccagg aacgcttgat aaactgcgtc agtttgccta tgagagtgac
     3661 cagtctttat tagaagcagc ttcaaatcta ttgatgtccc ccttgaaagg taaagcagca
     3721 caagctatca tggacttggc caatattctc ggtcagctac gtcaagacct agatcagatg
     3781 agtatcaccg acttagcaga agctttgtta gaaaaaacag gttatttaga tagcttacgc
     3841 ctgcaaaata cattggaaag ccaagcccgt atcgaaaata tcgaagaatt cttatctgtt
     3901 accaaaaatt ttgacgagtc aagtgcctct caagaagaag acgaaaccgg tgttgatcgc
     3961 ttgggacgtt ttttaaacga tttagcactg attgctgata cagatgattc ccaagcagaa
     4021 gctgctgaag taaccttaat gaccctacat gctgccaaag gcttggagtt ccctgttgtt
     4081 ttcttaattg gtatggaaga aggtgtcttc cccttatcac gtgcctctga agatccagat
     4141 gaattggaag aagaaagacg tttagcttat gtcggaatca caagagctga agaagttctc
     4201 tttatgacca atgccaacac cagaaccctc tttggtaaaa gtagttataa ccgtccaaca
     4261 cgcttcttga aagaaatttc agaggaacta ctctcttata aaggattggc aagaccagca
     4321 caatcttcat ttggtgtgcg tttttctact gaaacacaca cacagtttgg acaagggatg
     4381 agcctatcag aagccttaca agctcgaaag gcccaggctc aagtaagaca atcagcacag
     4441 ccaatgcagg cacatacgat accatcagct tcaactagct cagttttacc gtttggttct
     4501 aatagctctg tagaagaggt cacttggcag attggcgata tcgctcacca taaaaaatgg
     4561 ggagacggga ctgtgcttga ggtttcaggc tccggaaaaa cgatggaatt gaaaatcaaa
     4621 tttccagaag ttggtctaaa gaaattacta gcaagtgttg ctcctattga gaaaaaataa
     4681 gaaaaaaatg agcttcctac tatataagtg agtaggaagc tcattttttt agatttctgt
     4741 atcatataaa agttaaaaaa ttttaagttt taggagcata tcagttgcat tttaaattta
     4801 aaggtgtata ataacaatat aaattaaata acgagtggtc actttataaa atattatcaa
     4861 aataacattt tataggattt tgaaatatcg gtattcaaac aaaaaataaa ctaacacttt
     4921 attatttagt ttatatagtc actttttgaa agtcgactat taaaagacta ttgcttgaag
     4981 aatgacagct gttattcttc cttggacaaa aggtacagga gtatctaaaa aggagaatta
     5041 tcatgggact tatttggact ttaatcgtcg gtgcgcttat tggtgtgatt gc
//
LOCUS       DAUVUY010000102         5030 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.103, whole genome shotgun sequence.
ACCESSION   DAUVUY010000102 DAUVUY010000000
VERSION     DAUVUY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.103"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(56..1390)
                     /locus_tag="VOX58_001718"
     CDS             complement(56..1390)
                     /locus_tag="VOX58_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HES5878931.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            complement(1523..2077)
                     /locus_tag="VOX58_001719"
     CDS             complement(1523..2077)
                     /locus_tag="VOX58_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HES5878932.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            complement(2116..3036)
                     /locus_tag="VOX58_001720"
     CDS             complement(2116..3036)
                     /locus_tag="VOX58_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HES5878933.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            complement(3329..3982)
                     /locus_tag="VOX58_001721"
     CDS             complement(3329..3982)
                     /locus_tag="VOX58_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HES5878934.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILLIGLSRIYLGVHYPSDILAGFVLGFGILHFIYPFYKQKRFEWRFLLKQD"
     gene            complement(3984..4547)
                     /locus_tag="VOX58_001722"
     CDS             complement(3984..4547)
                     /locus_tag="VOX58_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HES5878935.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     regulatory      complement(4626..4750)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4859..>5030)
                     /locus_tag="VOX58_001723"
     CDS             complement(4859..>5030)
                     /locus_tag="VOX58_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HES5878936.1"
                     /translation="FMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYEALRQQGFQGL
                     ELKHTYEHDKLK"
BASE COUNT         1673 a         1050 c          812 g         1495 t
ORIGIN      
        1 aacgttttta cattttcatt ataacacttt tcatcaaaaa gactaggcat ggagcctagt
       61 cttcctcaat aatctgttct atattagaca ggattaattt taatttcgta atacggccat
      121 ctttgacttt atcgttgatt agaactaaat gtttgtcttt gttatctatt tcgtaggctt
      181 ctttttgctc ctggcttgga atggttccga tacctgtcaa ataaaaacca gcaatggtat
      241 ctacatcatc tgattctagt tcggtatcaa aatagtcatt aaactcattt aaagtcatag
      301 taccaacaac aatataggta ttgtctccaa tctcatgaac aaattgttct gctttatcgg
      361 tttcatcatc gatttcaccg acgatttctt caagcaagtc ttccaaagtc acaagtcctg
      421 ccacaccacc atattcatct agcaaaatag ccatctgatt ttgggtattg cgcagctggc
      481 gtaagagatc atctacaaaa atggtttcgg gaacaaaaag aggttcttgt aacatttttc
      541 gcatgttaat ctgatcaaat ccctggcgga aacctgactc caagagacgc ttagtgtgga
      601 tgagaccgat aattttatct ttatccacat cataaacagg aatgcgtgaa aaactttgtt
      661 ttaagatttc ctgaatattt tcaagcggat catcgttaat gtcaatcatg aaagcatcgg
      721 tccttgggac catgacttca cgcgccatca tttcatcaag tgagaaaact ccttgcaaca
      781 tctcaatttc ttcagcatca agggtcgcct cactttttga taacatatac tcgatttcat
      841 cacgtgtcat ttgctcatct gcatcatcaa aggtcatagg ggtaagtcgg ctcagtaaat
      901 tagtagaagc tgataagagc catacaaagg gactaaccag tctccctaat ccaatgataa
      961 taggggctga aacaatcgcc aacttgtctt tgaggttcat ggcaatccgt tttggataca
     1021 attctcctaa aacaatagag acataagtca agaaaaccaa ggagatgata gtaccagctg
     1081 tccttgcggt cgctgaatta cctagccaac ctgagatcac cttacccaaa gaagctgata
     1141 aacttgctcc tgatagtaaa ctaataaagg taatcccaac ttgaatcgtt gataaaaaat
     1201 gattaggttc ctctaaaacc cgcaacaaac gagcgtattt tttatcaccg tctgctgctt
     1261 tttgttccac ccgagaacga ttgagagaaa ctaaggccat ttcactggct gaaaaaaaag
     1321 catttaacaa ggttaaaaca actaacaata aaaattgaat cactaagggc tgactcacag
     1381 ggtcttccat gttaatgtaa ctcctaaaaa aataatatag tgacttcatt atatcacatt
     1441 tatagccaaa gcctatggga tttcttcttt ttccaccgaa ataatcgcag aacttctagt
     1501 agacttgaca cagggataag agctaaacgt ctgctaattt ctccaacatg attaaaaaag
     1561 gtggggtatt cacctgatta agaggctgat aaagcattgc agaaaccttt ctttggtcta
     1621 attgcttgac aaaatctaat aaagcatctt tttccaaact gcctccatca tgcccataat
     1681 agaccataat agcaatacgc ccacctacca ttaataacgt tagtagtttt gataaagctt
     1741 ggagtgtggt atttggtaag gtaataatag atttatctgc gctgggcaaa taaccaaggt
     1801 taaaaatcgc tgcgcgcacc ggctctgtca catactgatc aaccgcttca tgacccgcta
     1861 aaatcagctc agcgttcgat aatcctagct gcgctaaccg ctcacttgtt tttctaatag
     1921 cttgttcttg cacatcaaaa gcataaacct ttttagctaa ttgtgccaaa aaggcagtat
     1981 catttccatt acccatcgtg gcatcaacaa ccacactaga cttatccaca acctccgcta
     2041 aaaaatcatg cgagagatga atggggcgtt ttagcatcac ttttctccta tccaaatccg
     2101 tcaaaatgag acaaattagt caactttaca gccctgaaag cttcctcgtc tttccatttc
     2161 tttatcaatg gcattcaata cttcccactt atttagactc cacatgggcc caatcaacat
     2221 atcgcgtggg gcatctcctg taatacggtg aatcacaata tgcttaggga taatctctag
     2281 ctggtcacaa ataatcgaaa catagtcctt ttgactcaac aactttaagc gtccttcatg
     2341 ataatctctt tgcattctag tattggtcat caaatgcagc aaatgcaatt taattccttg
     2401 aatgtcattg tctgttacac agcgtctcac attttcaagc atcatgtcat gagtttcttt
     2461 tggcaatcca ttaatcaagt gcgagacaat attaatgttg gggtaatgcc ttagtcgcct
     2521 caccgtttct ttatacaaat cataagaatg tgctctgtta attagcctag aagtttcttc
     2581 ataagttgtc tgtaagccta gctccactgt cacatgcatg cgttctgata attctgctaa
     2641 gtaagcaatg gtatcatctg gtagacagtc aggccgtgtg ccaatattaa taccaacaac
     2701 tccaggttcg ttaatagctt gttcgtaacg gtcgcgaatc acatcaactg tatcatgagt
     2761 attggtaaag ttttgaaaat aaaccaaata gcgattgaca tcaggccatt tacggtgcat
     2821 aaaatcaatt tctttataaa attgctcttt aattggagca tctggtgcta caatagcatc
     2881 tccagaacct gatactgtgc aaaaagtaca tcctccatga gctaccgttc catcacgatt
     2941 gggacaatca aagccggcat caatcggaac tttaaacatt tttgcgccaa atagttgacg
     3001 gtaatgctca tttaatgtct gatatctttt tttcatgttt ctattgtagc acaaatcatt
     3061 actagcaaga tagtcatcta tgcaaaagat agctcagtca ttagaattca ctacttctac
     3121 atcagcttac ttaatgatta cagatcaaca aaaatcacgt cttttctgac gttacatagc
     3181 actttaatcg ttttttatct aaacatattt actaatcatc agctcacatc atctaactaa
     3241 aactaaaata acactggcaa tcaagggggt gatgcttgcc aatgttattc taaatgattt
     3301 aaataagcct atgtcttctc aagaaaaatt agtcttgttt caaaagaaat cgccattcaa
     3361 atcgtttttg cttataaaaa ggataaataa agtgcaaaat accaaatcct aatacaaagc
     3421 ctgctaaaat atcacttggg tagtgtactc ctagataaat acgagataac ccaattaata
     3481 aaattagtat tataaagatc atgctagtca caaattgtaa tagttttgaa tgtagcctct
     3541 gataacaaat aatcaatagg ctaccaaaaa tcagcatgct acccatcgca tggccactgg
     3601 gaaacgaata gccaccagca taaaccaaat gctcaatagc aggtcttggt ctttggtaaa
     3661 agagcttgag agtcgtaatc aaaaatgctg caatggcacc atttgacaag ataaacaagg
     3721 cttcaatttt ccacttcata aagaataaaa ctaggaccga cacaataact aacaaaactt
     3781 gtgtcatgac atttccaaat acagtaacac ccctaaagaa ttgcgtcagc actataggaa
     3841 ggttgcctct aatctctgct tgaatagtat tatctagaag tgccaaacgt tcaggaaaaa
     3901 atttgaccgt atagcctatt ataacaaaga ttagcaatgc aaaagaagct attaaaaagt
     3961 gagtttgttt gtttgtcatc atattagtgt aagtatcttt ctaaaattgg tttacttgca
     4021 atatacacaa aataaaaggt aattgcaaat atcaacccct caacaagatt aaacggaata
     4081 accatagtca tcatatactt agtaacacca atataagctc tgatatcaat atttgcaaat
     4141 atagcatata aaggaatggc aaaagtataa ttgagaacaa ccatgccaaa cgttaataac
     4201 cctgtgccta gtaagctagc aaatacatat tgactaagcg ttttttgacg gttccaaact
     4261 aaggcaaaag ccgtcacaaa taaagcgata gcgataatat tcattgggag accaataaaa
     4321 tcattaaccc cacgattgtt caaaaagagt ttcaataagg acctcaataa caaaatcaag
     4381 taagcactct ttaagtccat aatcattaaa ccaaataaga caggaataat actaaattca
     4441 atttttaaaa aagctgcgcc tggaataatg gcaaagctaa ctagcatcaa tagaaatgat
     4501 atcgcagaaa gtataccaat cataatcatt ttatgtgttt ttgacattaa aaaattcctc
     4561 caattttttc aaattgaaag aagttccgtc gttattgtgt agctaaaata agcctacaca
     4621 aaaagcccgg tcttctctca tccagactat actgtcggtt gtggagttac accacatcag
     4681 cttgcgctcg cggacttcta gttacctagt caccgccggt cgggaatcac accctgccct
     4741 gaagacactt ctagcatagc agatttaaac ctagatagca atagctttga ctaaattttt
     4801 aaaagcgtca gctactgaca acgcatgttg ccaaaaactg acgcttttaa aaagtccatt
     4861 attttagttt atcgtgttcg taagtatgct ttaactctaa cccttgaaag ccctgttgtc
     4921 taagggcttc ataaatcacc atacaaaccg tatttgagac atttaaactc cgtacatgct
     4981 catcattcat cggaatacga agtgcttttt cagcatgttc tcgcataaaa
//
LOCUS       DAUVUY010000103         4960 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.104, whole genome shotgun sequence.
ACCESSION   DAUVUY010000103 DAUVUY010000000
VERSION     DAUVUY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.104"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            446..1324
                     /locus_tag="VOX58_001724"
     CDS             446..1324
                     /locus_tag="VOX58_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878937.1"
                     /translation="MIRKYDRTSTKNKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKIAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            1337..2599
                     /locus_tag="VOX58_001725"
     CDS             1337..2599
                     /locus_tag="VOX58_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HES5878938.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDNM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQNPTLSIKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTRA"
     gene            complement(2719..3345)
                     /locus_tag="VOX58_001726"
     CDS             complement(2719..3345)
                     /locus_tag="VOX58_001726"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HES5878939.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(3390..4805)
                     /locus_tag="VOX58_001727"
     CDS             complement(3390..4805)
                     /locus_tag="VOX58_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HES5878940.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGHPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGLYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(4806..>4960)
                     /locus_tag="VOX58_001728"
     CDS             complement(4806..>4960)
                     /locus_tag="VOX58_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878941.1"
                     /translation="YFNEIMEGTAQVRDRIARSKFIPEENLEQIKGLTQKVTKEIHHV
                     LAKGGI"
BASE COUNT         1567 a         1058 c          888 g         1447 t
ORIGIN      
        1 gatgtcatat cattgtccta actttctgaa acacttattc tcaataaaaa tcataggcaa
       61 gctaaccact gcttaaaaac caaaaagaag gtcagatctg taagatcagc aatgtagctc
      121 ttgatattta aagaaaacag gcaggaatct cacctgctag catatatctt acaacttatt
      181 atagcaaaaa atgatttttt ctgctagatt tcttagcatt attaaggaaa aagagaaact
      241 ctttttttaa ccatgataaa caatcctaaa aaaacgaaca ttataagaga aaagctttta
      301 acctcgtttc tcctaagctt tagcagggtt aaacgattct cttaaaacat tagctctttt
      361 ctttcagaaa tagtggtttt aaggtaaaat aatagtaagt tacctctatg agatagccag
      421 taaaaacata tcgaggaaat atcacatgat tagaaagtat gaccgcacga gcacaaaaaa
      481 taaatcacta aactggattt ggctaatcat tgcttttttt atgatttcaa gctttatagg
      541 tggctcttct tttactgagt cacttctaga cattttgcct gccatagcca ttggtggtac
      601 aggttatgct atttttcgtg ttagatctca tcaaaagcgg ctagcaaagg ccaagattgc
      661 caaacaatta gaagatctaa aagcaaaaat tcagctagct gatcgtaaag ttcggctgtt
      721 agacacttat ctggcagacc atgatgactt ccaatataac gtacttgctc agcaattatt
      781 accacaactt agtgacatca aagcaaaagc tattacgtta aaagaccagt tagatcccaa
      841 aatttatcgt cgtatcacca aaaaagccaa tgacgttgaa agtgatatta cccttcaact
      901 cgaaacactg cagattgcca caactcttaa ccctcagcct ttgaaaaccc catctcctaa
      961 tcttattaac aaagcacctg agctcaaacc gtattatgat aatattcaga cagatcatca
     1021 agctatcctt gctaaaatcc aaggagccga caatcaagaa gaattgcttg cgctgcatga
     1081 agctaacatg aggaggtttg aggatatctt aacaggctac ttaaaaatca aagaagagcc
     1141 taaaaattac tataacgctg ctgctcgttt agaacaagcc aaacaagcta tccaacaatt
     1201 tgatgaagat cttgacgaaa cccttcgtcg actcaatgaa agtgatctta aagattttga
     1261 tattagcctt cgaatcatgc aaggtgctac tcaaagacga acaacgcatc accaaaaaga
     1321 ctaaaggagt ccctttatgg cagattttaa ttttgacatt gaccaaattg ctgacaatgc
     1381 tgttgtcaaa accgataaaa ccaccgatat tatctcagac ttaccaactg acacaaatgg
     1441 ccaaatctct ttttttgaga aactaagtgc tgaccagcag acagctatta ctgccaaagc
     1501 cccggcattg gtagatacct ttttggcaga ccaaaatgcg ctacttgatt ttggtcagtc
     1561 tgctgttgaa ggggtcaatg caacggttaa tcatatcttg gcagagcaaa aaaaattaca
     1621 gattcctcag gttgatgatc ttttaaaatc aaccaatcgt gaactcaatg gttttatcgc
     1681 taaatacaag gatgccacac ctgtggacct tgataaaaag ccaaactttc ttcaaaaact
     1741 ctttaaacaa agccgtgata ccctgcaaga gttttatttt gattctcaaa acattgagca
     1801 aaaaatggat aatatggctg cagcggttgt caagcaagaa gataccttag ctcgcaatat
     1861 tgtctcagca gaattgttaa ttgaagataa tactaagtcc attgaacatt tggtaggcgt
     1921 cattgctttt atcgaagcta gtcaaaaaga agctagtcag cgcgctgcgg ctttacaaaa
     1981 agacctaaaa accaaagaca gtgctacacc agactatcaa ataaaagctg atcttttggc
     2041 aagaactacc gaggtcatca acactctcga acaacaacac accgaatact tgagtcgcct
     2101 ctatgttgct tgggcgacaa ctccgcaaat gcgtaatcta gtcaaggtct cttctgacat
     2161 gcgtcaaaaa ctaggcatgt tacgccgcaa taccattcca acgatgaaac tatctattgc
     2221 tcagttaggc atgatgcaac aatcggttaa atctggaatg actgctgatg ccattattaa
     2281 tgctaataat gctgccctcc aaatgctagc agaaacaagt aaagaagcca tcccagcatt
     2341 ggaacaatca gcacaaaacc ctactttgtc tataaaatct gtgacaagtc tggcagaaag
     2401 tttagtagca caaaataatg ggatcattgc ggcgattgat catggacgca aagaacgagc
     2461 acaactagaa agtgctatta tcagatctgc cgaaaccatt aacgattctg tcaaactacg
     2521 tgaccaaaat attgtgcaag cacttcttag tgaaggcaag gaaactcaaa aaacaattga
     2581 taaaacaact cgcgcataag ctaaacatca cctgtcacgc tacttaaaaa gcaagtcatc
     2641 agttaggtta atccctttga tggcttgctt ttttactact taacgaataa tcgccttgtt
     2701 tgatataaca cttatctttt atgatgttat tttcataata cgaacaaggc tagcacgctc
     2761 ggcctcttct aatttcaact caatatagtg aatcgtctct tccagttgcg ggataataga
     2821 atactctaag ccattgacgc gacgtctagt cttttcaatg tcatcagcca ttagttgaca
     2881 agttttttca acttcagcta accgtaagag cttttctaat agttcttttg tcttttggat
     2941 agtattgtcc atctcactat ttgagctgag gtaactataa gcaaactcac cttgttcttg
     3001 ctctcttgcc gtattagact ggacatggaa tttaggcaca ttgacactca tgatattttc
     3061 aatatcaatc cataaggtca cttcatggac tggaacagca aagagttctt ctaccattag
     3121 actattttca gatgcttttg ccaagacaaa ctccttcata ttggcagcaa gttctttttc
     3181 gatagtttga cgaagctcat tgttttcacg aattaaatca acgaagcggc gcatcaactc
     3241 atcccgcttg tcctttaata atttatgacc tcttgtagct gtcttcaagc gatttttcaa
     3301 gttactaagt tccatgcgtg ttggtttaac atttaatcgt gccattgata taactccttt
     3361 cgtcaaggac ttgaaattgc atggtcaact caatcatttg caacaaaaac ctttgtcatc
     3421 gtggtatctg ctttaggcaa ataacgatct aacatatcat ctttaatgcg ttttaattcg
     3481 gtacgtggca aaatcgacaa caattcccaa cccaagtcaa gactttcttc aatgctacgg
     3541 ttggtgtaca atccttggtt aatgtattct tcttcaaacc ggttggtaaa ggccacatat
     3601 agtttatccg tttcagaaag ggctgattca ccgagtacaa cagccaattc cttagcttgt
     3661 tttccttggg cataggctgc aaagagctga ttcattgtag cagcatggtc ttgtcgagtc
     3721 ttaccttcac ctgagccttt atcttttagg cgagatagcg atggcaagac attaataggc
     3781 ggtctaaaac catttttata tagttcttgc gacaaaataa tctgaccttc ggtaatataa
     3841 cctgtcaaat cggggatagg gtgggtaatg tcatcttctg gcatggttaa aataggaatt
     3901 tgagtcactg accctttttt cccgatcaag cgaccagccc gttcatataa ggtggagaga
     3961 ttagtataga gatagccagg ataacctcgt ctgcctggaa cttctctgcg agcggctgat
     4021 acttctcgta aggcttcaca ataatttgtc atatctgtca tgatgactaa aacatgcata
     4081 cctttttcat aagccaagta ttcggctgtt gtcagagcta tacgtggtgt tgcaatgcgt
     4141 tcaatagcag ggtcattagc taaattgata aataaaacag atcgatcaat ggcacccgtt
     4201 tcacgcaagt cattcataaa gaattctgcc tcttcaaaag taatacccat agctgcaaac
     4261 acaaccgcaa agttatcatc agagtttaga acagttgcct gacgagcaat ttgtgcggcc
     4321 aattcattat gaggtaaccc tgagcccgaa aaaactggta acttttgacc acgaacgagg
     4381 gtattgaggt gatcaatagc tgagatacct gtctggataa attcgtctgg gtaatcacga
     4441 gcgacaggat tgatcgcttg accatcgata tcaaggtatt tttcaggaat aagttctgga
     4501 ccgccgtcta tgggatgccc cattccatca aagatgcgtc ctaccatatc ttctgatacg
     4561 gctaattcca aaggatgccc tgtaaatcga accttggttt tggctaggtt gatgccagtt
     4621 gatccttcaa atagctgtac catagccttg tcaccttgga cttctaagac ttgaccttta
     4681 cgcctttctc cattatgcaa agtaatatct accaattcgt tgtagtgcac tcctgcaact
     4741 tgatcaacaa tcatcaaagg gccaaccact tcgctaaccg tccgatattc tttgagaacg
     4801 ctcatctaaa ttcctccctt tgctaaaacg tggtgaatct ctttggtaac cttctgagta
     4861 agccctttaa tctgctctaa gttttcttct gggataaatt tgctgcgtgc gatgcgatcg
     4921 cgtacctgag cagtgccttc cataatctca ttaaaataag
//
LOCUS       DAUVUY010000104         4928 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.105, whole genome shotgun sequence.
ACCESSION   DAUVUY010000104 DAUVUY010000000
VERSION     DAUVUY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.105"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..821)
                     /gene="cysK"
                     /locus_tag="VOX58_001729"
     CDS             complement(<1..821)
                     /gene="cysK"
                     /locus_tag="VOX58_001729"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HES5878942.1"
                     /translation="MTKIYKTITELVGQTPIIKLNCLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETVIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYA"
     gene            complement(949..1581)
                     /locus_tag="VOX58_001730"
     CDS             complement(949..1581)
                     /locus_tag="VOX58_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HES5878943.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            1637..2962
                     /locus_tag="VOX58_001731"
     CDS             1637..2962
                     /locus_tag="VOX58_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HES5878944.1"
                     /translation="MESIENSYGRLLLESQLPDSAKQLAQPLKSVVISRGKMICQRCH
                     YQLDEEARLPNGAYYCRFCLVFGRNQSDKLLYAIPPMHFPKGNYLVWSGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFNNRPLVIPKFIRSFN
                     LFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN"
     gene            2934..3599
                     /locus_tag="VOX58_001732"
     CDS             2934..3599
                     /locus_tag="VOX58_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HES5878945.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            3679..4227
                     /gene="raiA"
                     /locus_tag="VOX58_001733"
     CDS             3679..4227
                     /gene="raiA"
                     /locus_tag="VOX58_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HES5878946.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT         1707 a          836 c          869 g         1516 t
ORIGIN      
        1 gcgtaaagag cagctccaga agaaatccca accaggaagc cttccttagc tcctgttagt
       61 cgagcagttt ctaaagcatc tttcgattta acacggataa tttggtcata ggctttggta
      121 tctaacgtgt ttgggataaa tccagctgat ataccttgaa ttttatgtgg tccaggctct
      181 tgaccagata agacagcaga ttcttcagct tcaacagcat agataacagt ttcagggcta
      241 gcttttttca agacatgtga aacaccagaa agtgttcctc cagtaccaac accagatacg
      301 aatgcatcta aagaaatctc cttaaaagct tccaaaattt cttgagctgt tgttttttca
      361 tggatgcttg gattggcagg gttattaaat tgcataggca tccaagcacc tagttctatt
      421 gctaaagttt cagcttttgc aatagcccct ttcatacctt ctgctccagg tgttaagaca
      481 agctctgcac cataagcctg aatgatttgt cgtctttcca agctcatagt ttcgggcata
      541 acaataatga ctcgataccc tttagcagca cctacccatg caagaccaat acctgtatta
      601 ccgcttgttg gttcgataat aacgtcacca ggacttatca gaccttcagc ttcagcagct
      661 tcaatcatcg ataaagcaat acgatcttta acagaagatc ctgggttaaa agcttctaat
      721 tttacataaa cgtcagcagc ttcgtttgga attaaacagt taagtttgat aataggtgtt
      781 tgacctacta attctgttat agttttgtaa attttagtca tagtttagcc tcttttcttg
      841 atacaaaata aatacgctgt gtatttaagt ataaggagag atattattaa agtaaaatac
      901 agatttacta tcactctgat aataaagcca tatcactttt gttgatgatt aatcacaggg
      961 gaattcaata actttggatc cgattttctc acagcttatc tgaccatgat aataatttgt
     1021 taaagtatca atgagatttt ccacacgttc agtatcacaa taaagtgttg tctttatcgt
     1081 gtctgaaaac ttagtttctg tttcggttaa agccaattga tctagcaagt tagagtatag
     1141 ttggtattgg ggataagaaa gtgtgatttc tagtcctatc tgctgtttaa cttcaatgat
     1201 gccaaatcgt ttaatagctg ttgcggtcat gttagagtat gctctgatta gcccacctgt
     1261 tcctaattta ataccaccaa agtaacgcgt taccacaact actacatttg ttaaacactg
     1321 cttttctaac acagaaagga taggtattcc agcagttcct gaaggttcac catcatcact
     1381 tgagcgcttg atctgactgt tgttaccgat aatcatcgca aagcaggaat gattagcttt
     1441 gtaatgttct tttttgatag cattaacaaa attcttacca tcttcttcag tgcttactcg
     1501 tttgatatga caaatgaaac gagatttttt aatactttct tcgaaaaaac cactggcttt
     1561 gattgtctta aaatgttcca taaaaaaatt gtacaagaaa agataaaaat tagcaaaaaa
     1621 aagcgaataa taaagtatgg agtctattga aaatagttat ggacgattgt tgctagaaag
     1681 ccagttacca gattcagcaa agcagcttgc tcaaccttta aaaagtgtag taatatcgag
     1741 aggaaaaatg atttgtcaac gttgtcacta tcagcttgat gaagaagcac gattgccaaa
     1801 tggagcctat tattgccgat tttgtcttgt ctttggacga aatcaatcag ataaattgct
     1861 ctatgctatc ccacctatgc attttccaaa aggtaattat ctggtttgga gcggtcaact
     1921 aactgcttat caagagatga tttcccaaca attattaatc aatatgcaaa atcaaaaaac
     1981 aacattagtt catgctgtaa ctggtgcagg aaaaacagaa atgatttatg ctgcgattga
     2041 ggcggtaatt aacacaggtg gctgggtttg cattgccagc ccaagagtag atgtttgtgt
     2101 agaggtggct actcgtttat cccaagcctt ctcctgcagc atctgtttaa tgcacgcaga
     2161 gtctcttcct tatcaaagag ctcctattat agtagccaca acgcatcagc tgctaaaatt
     2221 tcacaaggct tttgatctct taattattga tgaagtagac gcattccctt ttgtaaataa
     2281 tattcaactt cattatgctg caagtcaggc tttaaaagaa ggtggcgcaa agattttatt
     2341 aactgcaact tcaactagaa ctttagaaag aaaggtaaac aagggagaag tagtcaagct
     2401 gacattggcg agacgcttta ataataggcc attagttatt ccgaaattta taagaagttt
     2461 taatctattt aaaatgatac atcggcaaaa acttcctcct aaaattttaa aatacttaaa
     2521 aaaacaaaga aaaacaggct atcccctttt aatttttcta cccactatta taatggcaga
     2581 atctgttaca gcaatcttaa aagagttatt gcccgccgaa caaatagctt gtgtttctag
     2641 tcaatcacaa aacaggaaag aagatatcac agcttttcgt caagggaaaa aaacgatttt
     2701 aattacaaca acaattttag aaaggggtgt gacatttccc caaattgatg tttttgttct
     2761 tgggtctcat catcgtgtct atagttccca aagtcttgtt caaattgctg gacgagttgg
     2821 aagatctata gacagaccag acggaacgtt atatttcttt catgaaggta tcagtaaagc
     2881 aatgctacta gcaagaaaag agattaaaga aatgaattat aaagggtatt cgcatgatct
     2941 gtctactatg tcaacaaatt agtcaaacac caataagtat tacagaaatc atctttttaa
     3001 ggcgtatctc ttcaccgatt tgtcaacaat gtcaaaaaag ctttcaaaag ataggaaaaa
     3061 gtgtttgtgc gacatgttgt gcaaactcag atataatagc ttgtcgagat tgtctaaaat
     3121 gggaaaacaa aggatacaat gtaaatcata gaagcttata ttgttataat gctgctatga
     3181 aagcatactt cagtcaatat aagtttcaag gagactattt attaagaaaa gtttttgcag
     3241 tagaacttgc cgatgttatt accaagtact ataaaggcta tatcccagtc ccggttcctg
     3301 taagtcccgg ttgttttcga gaaagacaat ttaatcaagt gagcgctatt cttgaggcag
     3361 ctaatgttag ctacctttct ctttttgaaa agctagataa tactcaccaa tcttccagaa
     3421 caaaaaaaga gagattatta gtagaaaaat cttatcgact actaaaagta tcaaacattc
     3481 ctgataaaat ccttatagta gatgatattt atactactgg tagtacaatt atcgctctta
     3541 gaaaacaatt ggctaaagta gcaaatagtg acattaaaag tttgtcaatt gcacgttaaa
     3601 acttgcaaat tttcatgaaa gcagtataat agtattaaag ataaagtaag cgcttcaagc
     3661 gcgagaaaga ggtatcctat gattaagttc agtattcgtg gagaaaatat cgaagtgaca
     3721 gaagctattc gcgattatgt agaatcaaag cttactaaaa tcgaaaaata ttttgctaaa
     3781 gaccaagaaa tagatgcacg tgttaattta aaagtttatc gggagagatc ttcaaaagtt
     3841 gaggtaacga tcccattaga ttcagtaacc cttcgtgctg aagatgtttc acaggatatg
     3901 tacggctcta ttgatttagt tgttgataag atcgaacgcc aaattagaaa aaataaaacc
     3961 aaaattgcta aaaaacatcg cgaaaaggtt ccaactggtc aagtctttac aactgagttt
     4021 gaagctgaag aagtcgatga gataccagaa gtacaagttg tacgtactaa aaatgtgaca
     4081 ttaaaaccaa tggatgttga agaagcgcgt ttacagatgg aactattggg acatgatttc
     4141 tttatctaca ctgattctga agatggtgca acaaacattt tataccgtcg cgaagatgga
     4201 aacctaggat taatagaagc taaatagttc acaactaaaa cctacaggga tgattatacg
     4261 tattttaaaa gtgggaaaaa gctattcatg tttcgaggtg acatgatgaa aatgagatta
     4321 agtgatagat acgacagtca ttatataaca aaagtatcta ttacagttcc tctttaagta
     4381 aatcttacgt aaaattagtt tgaaaaaaag attagtccca attctgggct agaaattctc
     4441 aaaaaatatg aaaaagcgcg ttttcagaat ataaagcgcg tttttttctt tttccattta
     4501 acaataaaaa actcatgacg aacatggtat cataagagaa tttttacttt ttaaaaaaag
     4561 gtcaaagact ttctgacacc aaataaaaat agaatttttt ctaactaaaa ataatagcac
     4621 aatctttggc agcaatctga attgtttttc cataaaagat ttaagcactt acgtttgctt
     4681 aaaatatagg ttatgtgacg tgcgtgcaag atcagattat aacaaaatag ttccaaataa
     4741 gcctatcact gagaaagcat cacagaaggg ttaaactttt tttgtattag tgttttatca
     4801 gtacaaccta acacttattt ttataaaaaa ataaaaaagc tattgacaat cttatacaag
     4861 ataacgtata ctaataaagc tgttgtaaat atggtaaagc aatagatgct atcaggaaaa
     4921 aatgtgaa
//
LOCUS       DAUVUY010000105         4819 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.106, whole genome shotgun sequence.
ACCESSION   DAUVUY010000105 DAUVUY010000000
VERSION     DAUVUY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.106"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            17..283
                     /locus_tag="VOX58_001734"
     CDS             17..283
                     /locus_tag="VOX58_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5878947.1"
                     /translation="MNHFGEIFKTFRESKGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMSIDEFMYAVHDFHRDDLNELLSKSEGFRNNSR"
     gene            complement(1006..2505)
                     /locus_tag="VOX58_001735"
     CDS             complement(1006..2505)
                     /locus_tag="VOX58_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HES5878948.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDPSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVTKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVEEKPQNKKGLNRS"
     gene            2752..4392
                     /locus_tag="VOX58_001736"
     CDS             2752..4392
                     /locus_tag="VOX58_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878949.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDRKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALAPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(4481..>4819)
                     /locus_tag="VOX58_001737"
     CDS             complement(4481..>4819)
                     /locus_tag="VOX58_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HES5878950.1"
                     /translation="HRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRT
                     HQIRVHLAHQGHVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTQEDICVQAKSATFD
                     AVLNAQKARK"
BASE COUNT         1469 a          894 c          964 g         1492 t
ORIGIN      
        1 gtggtggagg tctgtgatga atcattttgg agagattttt aaaaccttta gagaatcaaa
       61 gggattacga ttaaaagatg tcgcaaaggc tggtatatca acctctcagc tgtcgcgttt
      121 cgaaaaaggg gagacggatt taaccatatc gacatttatg ctgattttgg atgaaagtaa
      181 catgtccatt gatgagttta tgtatgctgt ccatgatttt catcgtgacg acttaaatga
      241 actcttatca aaaagtgagg gatttcgtaa caattcaaga tagtgatggc ttgaagcagt
      301 tacttcatgc tcaactagca tcaactgata agaaggaaat gttcacggat atcttttggc
      361 atatctagca tgtctaagtc tgctatggca tgccattttg ggagtccctc acgcgactct
      421 gtcagtagct ctccttcaaa ggattcacat agaaagtcgt aatagacata gcactctttt
      481 tttgtcaggc ttgataaagc tggaaatacc ctttaattga aggtttaaag ctgtcagacc
      541 tatttcttct tttagttctc ataaagccgc ttcaaagaaa gattcacagg gctcgacttt
      601 accttctggc tggacccatc ctggtaagtt atcgtgttgt ctatggagtc ataaaatcat
      661 atcgttatgt ttgacacaca tattgcccca gtctctaacc gttgctatca ccagattcac
      721 caccttttag tataagaaaa aaggggtgaa agaggtttct tgttgttttt ttggcttaag
      781 atttctttaa gtcttatcta gtatactaat gtcattcctt aataggataa gtatttggtt
      841 gtagaagtat cgtcttaaac taacgctatc tcaaccgatt cccaacttat tcacccctcc
      901 tataccgata aggtctcaca aagtgtgaga ccttattttt tgacttcagc tctagttatg
      961 gagaaacttg ttgatacaag cttttttggg ttatgggtta tggtattatg atctattcaa
     1021 gcctttcttg ttttggggtt tttcttcaac caacttggct tcaatagctt ttaagcgttt
     1081 gaaggttttt tcagcgcgat ctagtgaaat cttatcagct ttttcttgag cagctttggg
     1141 gtcactgacg actaagtcac tgatttcttt ggtgcttttg tcttgttcgg ctatccaggt
     1201 tttttccaag cctttcatct catcaatcac tttagttcca aatggttttg gatgagcagc
     1261 cacgagatca ttaatgtgtg acaccgtcca ataccatgac ttatcgttat attgggtgct
     1321 tttttcttga taggcttcat aggttctact gatattgcca aggtatggca agtaaggggc
     1381 attacgagga ctgccaatag atagccacat gacaccacca cccaattcag caggaatgtc
     1441 ttttttcagt tggaaaatat gagcttccat gacattagga tttgagatgg ggtaagcata
     1501 acctttaaca gcttttttgg attttggttt acccttaccg tctaatgcca tctgatcaag
     1561 tggtttaaga tccagcccct caaaacgatt acgttgcagt ttcatcgcat cttctagact
     1621 aaaggtctta tcagtgcttt ggagcaactc ataattgctg tctttataag ttacttttga
     1681 atcagggtca agtgatttaa tccctgagaa agagcgagag cggttggcat cgtttagtgg
     1741 tggattgtaa gatttggcaa tatgaaattt accatcaact tcggtataag atttggcttt
     1801 tttagtgact ttttccacat cttctgaggc aatcgtattt tccttatcgt tgaaatcgac
     1861 atgtccgaga tagaaggtat ttggaaaaac agcgtattta tcatctggga atttgatggc
     1921 aacgtattgg tgaccagaga gaatttccat gtaccagatg ccatctttgt cagccaaggt
     1981 aacaatattt ccttcagcag ctcctttttc agtgactatt ttagcaatca atgcgacacc
     2041 ttcacgggct gttttgacac ttggtaaaat gacactggcc atagatgatt ccgctaagcc
     2101 atttttgacg taaggatcaa ttttttggat ggcatcatta gctgaagctg atactgttgc
     2161 agacatagag acgccaaact cattaaaacc agcttcgtca taaacgcctt tattaggtgt
     2221 tacatcagga atcgcactat agcgataaga atgctcaggc aatgggtact caaaaccatt
     2281 agaggggtct ttccattttt cgccagcagg attgtccttt gctagacgta caatgaagtt
     2341 tttattatga tgtggctcaa gatcttctgt tcgaccataa agaagagaac cgtctttggt
     2401 taaatctttg ccaataataa aaccagtaca agcataacta acactttgaa gcgaaaaggc
     2461 tgtcagaata cttaaaactc caagtgatat ttttttgttt atcatgcgaa tcctcctagt
     2521 aaacgataac gctttcacag acattataaa atgctctttt taaaaattca agttttttat
     2581 gaagtgtttt gaaattgtta gctatctaaa tattatgaga attctctctt gaattttggc
     2641 ttaacggccc ctaaaaacgt ataggcagtg aactatctag gtttcttcga aaatccatta
     2701 gccatttatg atataatagt aaagaaaaac atagaaagtt ggggtctgtt gatgaccaaa
     2761 ccatttcatc ataagaaact aaaacaaata actataatag ctgcaactag ccttttttta
     2821 ttcctgatcg gtggtgcctt ttactactct aaaaatcatt gtattaatgc ctatcttaaa
     2881 gctcgatcag cccaatcagg accagttttt gaaaatatca aagcatatct agtctgggat
     2941 gatactaatg agcagatcac aaatgacgag gcgatgtata ctaagtttag aaggtatagt
     3001 caaaaagaat tgaggcaaaa aaagcaggat ttaaaagccg ctagtcaaga tagtgccgtt
     3061 caagtaaagt ctgttggtcg tcgtttttgg atttttcctg attatcggat tgccataaaa
     3121 cccatggatt taacgataaa aacgaatgtg cctcaagcag acgttctttt aaatcaaaaa
     3181 aaagttgctg tttctgattc agaacagttc tcagtaaagc ttgatcggct accaacggca
     3241 gaatataccg caagtatcag aggcaaacac aacgggcgaa acattaaagt caacaaatca
     3301 tatgatggtg ataatcccgt gctagatttg agtgtgtctt ttagaacttt tttagtaaca
     3361 agcaatgcta agcaaggaga tctttacttc gatgataacc atattggcac attaaaagat
     3421 ggtcaattac aagtagaaga ttaccctgtt acagaaaacg cacaagctta tatgaaaaca
     3481 acattcccag atggtgagtt aagatcacaa aaatatgctc tagctgatgt tgaagaagga
     3541 gcaaccctgg agattttagt tacagatctt ttagaagagg acaaagcagg ggagctatta
     3601 gtatcagctt ttgatcagct gatgcactac ctcagtacag gtcaagactc atctaactta
     3661 cgtagcgtct ttgaggcagg gtctagcaac gcattttata ggggattgaa agagtccata
     3721 aaagcgaaat ttcagacaga tacaagaaaa gccagccgtc tcaatattcc atctatcctt
     3781 ttgacaacaa tgactcaagt gggtaaaaca acttacgtgc ttgatttcac agctacctac
     3841 gaatttttgt atgacaactc aacagatcct gagcagcata cctctggaca tattaatcaa
     3901 gacttgactg gaaaagtgac tgtaaaaaaa gttggacagc attaccttat cagccagtcc
     3961 ggctctaaaa atattactgt tgttaaagag gacaatcaac tcaaagcgcc atctgtcttt
     4021 cctgagtcta ttttgggaac gtggacaggc caagccaatg gtttaagcat ccatatgtct
     4081 ctagcatcag atggaacaat tacgactaaa gttgaagatc gaaaaggcaa ccgttctaaa
     4141 gaaactcgga cagctaaaat tagtaaagtt gaagacaaag gcaatggttt ttatctttat
     4201 acgccagatc ctggaagtga cataagcgcc ttagctccag aaggaggatt ggggggtgca
     4261 aatgtcaaat atgcttatgg tttcaaaata tctggtaaaa cagcctctcc agtggtgtgg
     4321 caggcagcat taacacatga atttgattat accaagccac tttcgggagt aactttgcaa
     4381 aagcaaccat aaaggatgca aagccacaaa gaacagtaga aacaatgtta tcactcaaat
     4441 acagtgctat gaaaacagat aaactcaaaa ggctaggaga ttatttccta gccttttgag
     4501 cgtttaagac tgcgtcaaat gtagctgatt tagcttgcac gcaaatgtct tcttgggtta
     4561 gagggtgttt taaacgtaat tgataagcat ggagcatcag tcgagcacaa tctttttttc
     4621 cattagaata caaaggatca ccaaataaaa catgaccttg atgggctaaa tggactcgaa
     4681 tttgatgagt ccgtccagtt tgtaattgac aattgagtaa gcttgccgtt ttgtggaagt
     4741 ttttgactag agttacctca gtaatagctt tcttgccatt tatcggatcg acaacacgtt
     4801 ttctgcggtc atgtcgatg
//
LOCUS       DAUVUY010000106         4719 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.107, whole genome shotgun sequence.
ACCESSION   DAUVUY010000106 DAUVUY010000000
VERSION     DAUVUY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.107"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..89)
                     /locus_tag="VOX58_001738"
     CDS             complement(<1..89)
                     /locus_tag="VOX58_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HES5878951.1"
                     /translation="MIRPTSYESLAALIEKEDKLVLFFTADWCP"
     gene            complement(86..379)
                     /locus_tag="VOX58_001739"
     CDS             complement(86..379)
                     /locus_tag="VOX58_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HES5878952.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            835..1902
                     /gene="pepA"
                     /locus_tag="VOX58_001740"
     CDS             835..1902
                     /gene="pepA"
                     /locus_tag="VOX58_001740"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HES5878953.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPNIENIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            1950..2720
                     /locus_tag="VOX58_001741"
     CDS             1950..2720
                     /locus_tag="VOX58_001741"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HES5878954.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRNLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(2803..3111)
                     /locus_tag="VOX58_001742"
     CDS             complement(2803..3111)
                     /locus_tag="VOX58_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878955.1"
                     /translation="MYVFIEPNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLALIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(3298..4494)
                     /locus_tag="VOX58_001743"
     CDS             complement(3298..4494)
                     /locus_tag="VOX58_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HES5878956.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEEVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGEISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            complement(4553..>4719)
                     /locus_tag="VOX58_001744"
     CDS             complement(4553..>4719)
                     /locus_tag="VOX58_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HES5878957.1"
                     /translation="IFGHPSNAKSIIVLQKQTDHPMETFVYPIRDLKLAENIHDFMEN
                     FKKWKLSNVN"
BASE COUNT         1469 a         1023 c          859 g         1368 t
ORIGIN      
        1 ggacaccagt ctgccgtaaa aaataacacc agcttatctt ctttttcaat aagagccgct
       61 aatgactcat aagaagtcgg tctgatcatc ccttgtcctc ctcaaaacta atttgtcgac
      121 tgtgataggt gaattggtac tgtttgccgt ttgacataac aaccccacct gaaatataat
      181 cttgtttgac ctgacaagga ttgatatata aaacttcgat ttgtcctaaa ggtgaaaaaa
      241 aggtgcgtaa atcccttgtc atttgtcggc gctgcctgtc ttggcaataa tctttgataa
      301 ctattcccag gccaatagct aaactaaatc caaagatacc tgaaatcata cctattttct
      361 ttttactcat atccctcatt ttatcataaa aagatcaaat tggacgatct gaaataagaa
      421 aactaaagat ttctactgtt atgtattgac aaaaatagta aaaacaacta aactaaaagt
      481 acattgatta ggtgtggttt attattaatt taaccacgct ataaaaaata ctgattaacc
      541 taaaatggaa ggttctcctt atgactccat tatccttact tttcgctagt gaagagtctt
      601 ttcgcatcct aagcgcccta ctattcttat taaccagctt gtgctttatc attttaatga
      661 tttccttgct gcctaaaaaa acaggttatc gccaaaagac aaacacttct tccagagatc
      721 tctagtctct gggatttttc ttttctgtga aacccattct tacttaaaaa gtggtagaat
      781 ataaggagat gaccgcgttg aactggtctg aaacctttta aatggagatg aaaaatgaca
      841 gacttattct caaaaatcaa agaagttacc gaactggatg gcattgcggg ctatgaacat
      901 agcgttcgtg actacctacg caccaaaata accccgctgg ttgaccgtgt tgaaacagac
      961 gggcttggtg gcatttttgg tatcagagat agtaaagctg aaaaagcccc ccgtatttta
     1021 gtagctgcgc acatggacga agtcggtttt atggtcagtg atatcaaagt tgacggaacg
     1081 ctacgcgtgg ttggtatcgg tggttggaac ccacttgttg tcagttcaca acggtttacc
     1141 ctttacacac gcactggcca agttattccc cttatttcag gatcggtacc tccccatttt
     1201 ttacgtgggg caaatggctc tgctagtcta ccaaatatcg aaaatattgt gtttgatggt
     1261 ggctttacgg ataaggcaga agctgaaagc tttggcatta caccgggtga tattattgtc
     1321 cctcaatctg aaacgatcct aacagccaat caaaaaaata ttatttcaaa agcttgggac
     1381 aatcgctatg gcgttctcat gataacagaa atgcttgaag cgttaaaagg acaagacctt
     1441 aacaacaccc taattgcagg tgctaacgtt caagaagaag ttggtctgcg cggagcccac
     1501 gtctcaacca ccaagttcga ccctgaactc tttttcgcag tagattgttc gcctgctggt
     1561 gatatttatg gcaatcctgg aacaatcgga gatggtacct tattgcgctt ttacgaccca
     1621 ggccatgtca tgctcaaaga tatgcgcgac ttcttactga ctactgctga ggacgctggt
     1681 gtcaatttcc aatactattg tggcaaggga ggcacagatg caggtgctgc acaccttcaa
     1741 aatggtggtg tcccatcaac aaccatcgga gtctgtgcac gctacattca ctctcatcaa
     1801 accctctacg ctatggatga tttcgtagaa gcccaagcct tcttacaagc cattatcaaa
     1861 aaactggatc gctcaaccgt tgacttgatt aaacgttact aaactagcgt tagcctgatt
     1921 aaccgtaata tcccacaagg agcaacagaa tgaaaattgg cattattggt gttggcaaaa
     1981 tggctagcgc tatcatcaaa ggccttaaac aaacacccca tgaacttatc atttcaggat
     2041 catctttaga acggtccaag gaaattgcag agcagttaac actgccttat gctatgtccc
     2101 accaagacct tattgaccag gttgatcttg ttattttagg catcaagcct caactatttg
     2161 aaacggtact caaaccgctt cacttcaaac agcctattat atctatggca gcaggcattt
     2221 cccttcaacg actagcaaca ttcgtaggac aagaccttcc gctgctacgt atcatgccaa
     2281 atatgaatgc acaaattctc caaagcagta ccgctttaac gggaaatgct ttggtgtccc
     2341 aggaattaca agcacgtgtt cgaaacttaa cagatagctt tggtagcaca tttgatatta
     2401 gtgaaaagga ttttgacacc tttaccgctt tagcagggtc aagtcctgcc tatatttatc
     2461 tctttattga ggctttggct aaggctggcg tcaagaatgg catgcctaaa gcaaaggcgc
     2521 tggagattgt tactcaaaca gtcttggcta gcgccagcaa tctcaagacc agttctcaaa
     2581 gtccgcacga tttcattgac gctatttgta gccccggtgg cacaactatt gctggtctaa
     2641 tggagttaga acgccttggc ctcacaacta ctgtcagctc tgccattgac aaaaccatcg
     2701 ataaggctaa aagcttgtaa aataagtgcc ccaatacaaa tcagacaaca atacaaacac
     2761 ctccgttgac cagacaggaa actcaaccgt tagacaagtc atctaacaaa gaacctgact
     2821 cgtatcaaac ggaggttttt gactattagt tgttattttc cctagtgcct ccattttctt
     2881 agccaccaga cttctgataa tcaagtaaag gtaagcaaaa atcaaagcta gcttataaga
     2941 aacaaggtct cgaatggtta agcaagggaa gaaaaagaat aaggcaacaa atagaacaat
     3001 catcaggtaa aagcagtaaa cttgtgtctg ttttcgaatc gcacgattac gctcatcata
     3061 aaccgttatc aaatagtctt ttagatcatt gggctctata aacacataca agaaaagcgt
     3121 tgaagcaatt aaaaacttct aaataaggct gacatccaaa tcgcctagca caagattaca
     3181 aaaagcaagg agactgatca gaactaatac ctaggcacca taactaacag ataaacctca
     3241 taaaaaacca agtaaagtgt tgccttactt ggttaatcta tgaaaactta gtctctttta
     3301 ttttgtattt ttcaaacgct caacgtcacg cgcgatgcac aattcctcgt ctgttgagat
     3361 aacaagaacc ttcacttttg actcaggagt tgaaatttca ccacggtatc caaaaacatt
     3421 tttctcagga tcaatgtcca ttccgaacca tgttaagcca ccaataacat cttgacgcat
     3481 caatggtgca ttttcaccca taccagctgt aaagaccaaa gcatctgctc cattcaaaac
     3541 agcgaaatat tgaccaatgc attttttaat gcgatcaata aagatattgt aagctaaaac
     3601 agcatctgga ttatcttctt gtaaaccagc ctcgatatca cgcatatcac tagaaatgcc
     3661 tgacacccca ctaagacctg attttttgtt taacatattc acaacgtcag cagcgtcttt
     3721 taattctggg tcttgctcaa ttaaataagg aatgatagct gggtcaatgt caccagaacg
     3781 tgttcccatc ataggaccag caagtggtgt aaagcccatc gaggtatcaa ctgatttacc
     3841 gtggtaatta gctgtaattg agacaccatt tccaatatga gcagtaatta gttttaattc
     3901 ttctaatgga cgaccaagca ttttagcagc ttcttgcgct acatatttat gacttgtccc
     3961 atgcgcccca tatttacgaa ccttgtagtc cgtataatat ttttgaggaa ttgggtaaag
     4021 gtaagtatgc tttgccatgc tagtgtgaaa tgacgtgtca aacacacaaa cactggtaat
     4081 atctggcaaa atatcacgga aggcacgaat ccctgccgcc gctccaggat tgtgaagtgg
     4141 agctaaaacg gataattctt caatctgttc aagaactttg tcattaacca caacggattc
     4201 tttgaaaagt tcaccaccag caacaacacg gtgtccaact cctgtaattt catcataagc
     4261 tgcaatgatt ccaaagtgaa tcaaatcgtt caataagatt ttaacagctt ctgtatgatc
     4321 gtgaatatca agaatttgct cttctttttt gccatcgtac ttaactgttg aaatagaatc
     4381 cttgagaccg atacgctcaa taattccttg tgctaacact tcttcttctg gcatctgata
     4441 aagttgccat tttaaactag aactacctgc attaattgca attgtctttg acatttttaa
     4501 cctctcttga aatcgttttc tgtgatgctt ttttattata acaaaaaatc aattaattga
     4561 cattactcag tttccatttt ttgaaatttt ccataaaatc atgaatattc tctgcaagct
     4621 tcaaatcccg aattggatag acaaaggttt ccattgggtg gtctgtttgt ttttgtaaga
     4681 caataatgga cttggcattt gagggatgac caaaaatag
//
LOCUS       DAUVUY010000107         4615 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.108, whole genome shotgun sequence.
ACCESSION   DAUVUY010000107 DAUVUY010000000
VERSION     DAUVUY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.108"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            308..721
                     /gene="yaaA"
                     /locus_tag="VOX58_001745"
     CDS             308..721
                     /gene="yaaA"
                     /locus_tag="VOX58_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HES5878958.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRIGDKISLPDQDLIITIVEPSQEEKEQFAEEIAEKTRVAALVKQMNQANKK
                     TSSKHKNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            723..1829
                     /gene="recF"
                     /locus_tag="VOX58_001746"
     CDS             723..1829
                     /gene="recF"
                     /locus_tag="VOX58_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HES5878959.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLAGYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDNLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQVDSDIH"
     gene            complement(1886..2749)
                     /locus_tag="VOX58_001747"
     CDS             complement(1886..2749)
                     /locus_tag="VOX58_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HES5878960.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIIGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
     gene            complement(2952..4433)
                     /gene="guaB"
                     /locus_tag="VOX58_001748"
     CDS             complement(2952..4433)
                     /gene="guaB"
                     /locus_tag="VOX58_001748"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HES5878961.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT         1574 a          923 c          760 g         1358 t
ORIGIN      
        1 tgtgttggta ctttactttt ttccttgttt atagcaactt aagtttatat cttgaaaatt
       61 gtcaacgttc caaaatataa aagttttgtg ttatacaact ttactagagg gacatattaa
      121 taataggatt tatacgtaga aaaatgataa acataagtac agcttttgtt aaaactaatt
      181 aacaagaact tcaaaactaa atagttattt ggacgagtat gatactttat aaatagctct
      241 tattcagcta ttattattta tttctgtttt atgctataat gacttgaatg acaaaatgga
      301 gatgacaatg atatacaaat tatttacaga atttatcacc ctacaagccc ttcttaaaga
      361 attaggaatt atccaaagtg gtggcgctat caagggattt ctcgctgaaa caaccgttct
      421 atttaatggt gaagatgaga aacgtcgagg caaaaaaatc agaatagggg ataagatttc
      481 tctacctgac caagacttaa ttatcactat tgttgagcct agtcaagaag aaaaagaaca
      541 atttgccgaa gaaatagctg aaaagacacg tgtagcggct ctagtgaaac aaatgaacca
      601 agctaacaaa aagacttctt ctaaacataa aaatcgtcaa tctactacca aaaaatcact
      661 aagagcaact aagaaaacta aaggtaaacc aactgcacct gtccgcttcc caggtatcta
      721 gtatgtggat taaagagtta gagttaaaac attatcgcaa ttatgaccac ctgcttgcct
      781 cattttcgtc gggtttaaat gtctttatcg gtaataatgc tcaaggaaaa accaattttc
      841 tcgaggcaat ctattttcta tcacttacta gaagtcatcg cacgagggct gataaagaat
      901 tgattcattt tgatcactca actgtttctc ttactggtaa gattcagcgt attagtggta
      961 ctgttgattt agaaatcaat ctatcagata aaggacgtgt gacaaagatt aatgcgctaa
     1021 agcaagcaaa attatctgat tatattggaa cgatgatggt tgtgcttttt gcaccagaag
     1081 atttgcaact agttaaggga gcacctagcc ttcgtcgcaa atttatcgac attgaccttg
     1141 gtcaaattaa gcctgtttac ttatctgagt tatcccatta taaccacgtg cttaaacagc
     1201 gtaatagcta tttaaaaagt gcccaacaaa ttgatgccgc ttttctagct gttctagatg
     1261 agcaattagc tggctatgga gcccgtgtca tggaacatcg tattgatttt atcaatgcgt
     1321 tagagaaaga agcaaatact catcaccaag ccatctcaaa tggtttagaa agtctctctc
     1381 tctcctatca gtcgtcagtt gtctttgaca aaaaaactaa tatttaccaa caatttttac
     1441 accaacttga aaaaaatcat cagaaagatt tcttccgtaa aaacactagc gttggtccac
     1501 atcgcgataa cttagccttt tacattaacg ggatgaatgc taactttgct agtcaaggtc
     1561 agcaccgcag ccttattctt tccttaaaaa tggctgaagt tagtttaatg aaggccttga
     1621 ctggagataa ccccattctt ttactagacg atgtcatgag cgagctagac aatacacgac
     1681 aaacaaagtt acttgaaaca gtaattaaag aaaatgtcca aacctttatc acaacaacaa
     1741 gtttggatca cctgtcacag ttgccagaag gtatccgtat ttttcacgta acaaaaggaa
     1801 ccgttcaagt tgattctgat attcactaat aacatcaaaa aaagggttca gttaaactga
     1861 gccttttttt gatattatgt ggcgattaag atttgaccac acctaataaa atagcaccta
     1921 cgataaaaca aataattcct gtgacaaccc aacgcatttc ttttttggtt ttggtttcgc
     1981 ctaggaataa aatgcctcca acaatcgaaa tgatagcacc taattgtgag aaactaaagg
     2041 caatagcaag tcctgctttt gatgcagcta aaagcatgaa aatattccca atgccccaaa
     2101 gtaatccaac aacgctattt ttaatcacat actggtcaat ggaaatttta aatgacatga
     2161 aagttatagc tcctaaaacc atacctactg ccatgggcaa aatgactgac aaaacttcaa
     2221 atttcatgat gttattaaat aatacagcat acatcacgta accaattgtt gaataagtga
     2281 gcgccctaaa tccttttgaa aaattatgaa ggtgattgac ttgtgcatta gcatcatctt
     2341 gtttactaga aaagtaaaaa ccaataatca gaagtaatag tgctagactt cctactacaa
     2401 attgcatagg tcttgtccat tcatgaaaaa cgaggacacc aatcaaactt cccagaacaa
     2461 gttgagagcc acttgacaaa ggattggcaa cggagactcc catgtattgc atggcatgaa
     2521 attgaccagt ttgaccaatt gaccagataa aaccacccaa tattccaaaa agccaaagtt
     2581 gcagtgtcat ctcaggtcta acaattaacc aaacagctaa tgaaaataat aaagccccaa
     2641 aagtcattcc tagagtttgt tgcgaaggtt ttcctccaat tttgttacta acaaatccaa
     2701 tgcttcccca tgtaaacatg ggaataagtg cataaaaaat accttccaaa atgttatctc
     2761 ctttatagta aatctttaaa gattataata attctatcat aaaatggttt catgtaaaga
     2821 ctaattatag cttatttact aagaaaaaac aataaaagtt atccgaaaat tgttattata
     2881 ttttatcatt ttttaatata aaaaaagagc tggcacaata tgtcagccct taaagttact
     2941 aaattaaatt atcagtgcac agaatagttt ggtgcttcat ttgtgatttg gacatcatgc
     3001 ggatgacttt caatcaagcc tgcacctgac atttcaacaa attgagcatt ttcatgtaat
     3061 tcttgaatgt cacctgcgcc aacatagccc atacctgagc ggataccacc taacatttgg
     3121 aagacaatat ctgaagcagc tcctttataa gcaacacggc cttcaatacc ttctggaact
     3181 agtttattag cttcatttac ggaaccttgg aagtaacgat cgcttgatcc tttcttcata
     3241 gctgcaatgg aacccattcc acgataagtt ttgaacttac ggccttggta gatttcagtt
     3301 tcacctggag cttcatcagt acctgcaaac attgatccaa gcataacagc attaccgccg
     3361 gctgcaagag ccttaacaat atcgccagaa tacttgatac caccatcagc aataatcgtt
     3421 ttgccatatt cacgagcaac agcagcagca tcgtaaatag cagtcacttg aggaacaccg
     3481 acacctgcaa ccacacgagt ggtacagata gatccgggac caatacccac tttaacaaca
     3541 tcaacacctg catcataaag agcacgcgca ccttcagcag tggcaatatt tccagcaatc
     3601 aaagtacgat ttgggaaatg agcacgaatc tcagcaattt tacgaagaac accagccgaa
     3661 tgaccatgag ctgtgtcaat aacaatcgca tctgcaccag cttcaaacaa ggcctcggca
     3721 cgctcaaaag tatctgaggt aacacctacc gcagcagcga ccagaagacg accaaattca
     3781 tctttagcag catgtgggaa ttcgattact ttttcaatat ctttgatagt gattaaacca
     3841 gacagacgac cgctattatc aaccaaagga agtttttcaa tacggtgttc atgaagaata
     3901 cgttctgcag tttcaagatc tgtaccaaca gcagccgtaa cgaggtgctc actagtcata
     3961 tgttcagaga ttggtgcatt gtaatcagaa ataaaacgca tatcacgatt agtaatgata
     4021 ccgaccaact tacggtttgc taaagtttca acaattggca caccactgat acggtagcgc
     4081 tgcattaatt cttcagcttc tgaaaccttg tgttcaggtg ttaagaagaa tggatcaatg
     4141 atgacaccat tttcagaacg ttttaccttg cgaacttctt ctgcttgctc agtaatagac
     4201 atgtttttat gaataacacc taaaccaccc gcacgcgcaa ttgcaatagc cattttacta
     4261 ccagtaaccg tatccatagc tgctgtaata ataggaatat ttaatgtcaa attgtctgcg
     4321 agttttgtct ttaaatcaac ttcatttgga agaacatgac tctctgccgg aataagcaag
     4381 acatcgtcaa aagtgtagcc ttttttcaaa aatttagtgt cccaatttga catccagaca
     4441 atcctctttt cttttatttt tagggaatat taccctggtc tattttttat tgttactatc
     4501 atatcacgct aaaatcattt gtcaataaaa tattcctaca gctgaagtca ctatcagcat
     4561 aatatattag tattatccgt tttttattaa tcaaaaaatt catttgggtc ttatt
//
LOCUS       DAUVUY010000108         4499 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.109, whole genome shotgun sequence.
ACCESSION   DAUVUY010000108 DAUVUY010000000
VERSION     DAUVUY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.109"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..931)
                     /locus_tag="VOX58_001749"
     CDS             complement(<1..931)
                     /locus_tag="VOX58_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HES5878962.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVNHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQ"
     gene            complement(1149..1793)
                     /gene="fsa"
                     /locus_tag="VOX58_001750"
     CDS             complement(1149..1793)
                     /gene="fsa"
                     /locus_tag="VOX58_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HES5878963.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            complement(1919..3418)
                     /locus_tag="VOX58_001751"
     CDS             complement(1919..3418)
                     /locus_tag="VOX58_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5878964.1"
                     /translation="MKIEDLMDKERKAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYLEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMGQETDFSQQLSDDILALLVFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVYLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            complement(3408..>4499)
                     /locus_tag="VOX58_001752"
     CDS             complement(3408..>4499)
                     /locus_tag="VOX58_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HES5878965.1"
                     /translation="LAIEAPSNTLLLKDSQGRVFEEGYETLVCAMGASPQSHYIETSQ
                     TNKVLVTKYYEESQASLKLIEASQEVLVIGAGLIGLDLAYSLSLQGKRVKLIEAAERP
                     DFYQTDAELIAPVMAEMSTHHVTFINNKRVTAIHEIEGKVVAHTEQGDTFQGDLAILA
                     INFRPNTHLLQGQVACALDKTILVNENLQTSQANIYAIGDMVSLYFGILGMDYYTPLI
                     NQAMKTGQALALHLAGYPIPPLQTVKVLGSSHFGYYRASVGVTEEEAELYMDTCSYLY
                     QNGDSKNLFWLKLIARKTDGILIGAQLLSKTNALVIANQLGQALALKVTDGDLAFQDF
                     LFLQGHSDLAYHLHEACLKLFEKRLRHED"
BASE COUNT         1287 a          950 c          890 g         1372 t
ORIGIN      
        1 cttgttctgc tttttcacca cgcgcttgaa gaccttgagc aaagcgctct gttacttcag
       61 caggcacttc aaaatcttga tgagtccatt ggtaagcttt tttggcaaaa gcaatgcctt
      121 ctgctccaag aggagcgccg tgaacagctg aagttccttg tttttcggca ccaaaaccaa
      181 taatggtttt gacttcgatg atggttggtt tctcagtttc agctttagct gcttcaatag
      241 cagctgcaat ttcttctaaa tcatttccat ctttcacaag gatatgttgc caaccgtaag
      301 cttcgaaacg tcctttcaca tcttctgtga aagccataga ggttggacca tcaagagaga
      361 tgtcgtttga atcatatagc aagaccaatt tcccaagttt taaatgtcct gccatacttg
      421 ctgcttcttg gctgacccct tccataaggt caccgtcacc attcaaagca aaggtgtagt
      481 gattaacgat gtcaaagcct ggtttgttaa atttagctgc taggtgagct tctgccatgg
      541 ccataccaac ggcatttgcg atcccttgac caagaggtcc tgtggttgct tcgacaccgt
      601 ctgtgtgatt cacttctggg tgacctggtg ttttagaacc ccattgacgg aagttcttta
      661 aatcttctac agataaatca taaccagcta agtgtaacaa gctataaagc atggcacttc
      721 catgacctgc tgataggata aaacggtctc tgtttgacca attacggctt gttttgggat
      781 tgatgttcat gaagtgattc caaagaacat aggccatagg tgctgccccc attggaagtc
      841 ctgggtgtcc agaatttgcc gcttgaatgg catccattga tagggtgcgg acagtgttta
      901 ctgccaactg gtcaattgca tcaaatgtca taagtaaaac ctctctgttt attacaacta
      961 aaattaaacg ttttctattg tctattgtat cacaaaaata gacgcttttt tcagaaaatc
     1021 ctccaaagtc cctaaattct ttttctctat tttgccagaa aaaaactagt ttggacatca
     1081 ttggtgaatt cttttggacg atgaatataa gagagcctgt tgacacagta gccacagact
     1141 ctttagcctt attttttaaa ggaagcccaa tcttccataa aggttttcaa accatttgtc
     1201 gttaagggat gttgcgtcat cttagcaaac aaggggtctg gaatcgtcgc aatgtgtgcg
     1261 cctaatttag caacagcttc cacatgggct gttgtacgaa tacttgctgc gatgatttct
     1321 gcttggaaat cgtacaagtc aatgatttca cgaaggtcgc tgattaattg ataagcatct
     1381 gtgccaatat cttccaaacg accgataaat gggctaatgt aagttgcgcc tgctttcatt
     1441 gccattaaac cttggcttac tgtgaaaatc aaggtcacat tagttttaat cccttctttt
     1501 gaaagaatgt ttgttgcttt taacccttct gtggtcattg gaattttaac gacgacattg
     1561 tcatgccact tagcaatgct tctagcttct tctaccatag catctgctgt taaccctgtc
     1621 acttcagcac tgattgggcc atcgacgatg tcacagattt ctttgataac tgtttcaaaa
     1681 tcacgtcctt cacgtgagat aatggttgga ttggtggtga caccatctac aacgccaagt
     1741 tcattaatcg ctttaatagc ggctacatta gctgtatcta ggaaaaattt catttcttac
     1801 ctccaaaaat tttcgatttt gttaagctct atttgataag ctttttcaaa tcatctacct
     1861 ttagactatc ctactttatt attataaaag tgtaacagtc cttagaaaag accaagtatc
     1921 acacagtttt gtgtgcaaaa ctagacttag ctgctgggct gagtgagagt tcctcttctc
     1981 gtgtattttt ttgctggaaa aggtctttga gaaaagcttc taaggcagtg agttcatagg
     2041 gggagctata ctgttttaac cggtaatagg gtaaatcttg tctcggtaaa tagacagtct
     2101 gataggtaat cagacagtca taaacttttt gatgatcata gggcattaat tgaaaatccc
     2161 tacgatgccc caaatgacgt ctaatggcca attccgcaat ggctgcttcc accttatttt
     2221 gccccaaggc aagccctact aaaagaggct catggtggcg ctcaatggaa aaaaccaaca
     2281 atgctaaaat gtcgtcactc aactgctgac tgaaatccgt ttcctgaccc atttgcagca
     2341 ctaaatggtt tatgataacc cttaaaagcg ctcttttatc ttcgcctact aattgataaa
     2401 ggtattggct attggttggt tggctaagga tagctccttt aaaaaagtaa gctttggaaa
     2461 aaaatatccc caagccataa atgatttcct cctttgtctg atggataatc acatgagcct
     2521 tctttaaaag ccataaggct tctgaaatgt gatcagcaat aggacctcca aaacccaaac
     2581 tatacttcat gctggcaata ggaagtagag gataagcatg caaaaagaca aagaggctct
     2641 tagcttcaaa agcatcaaat tcaagggcat agcgtcttaa gtaatgtaaa agcatgcttt
     2701 ctaagcgctt aaagaaaata ttagatgtca taaagtcact atctcttaaa aaatgatcat
     2761 tgtagccttt ttgaaaggac attcgcgctt gcgaaatcgc caaccaaatc aataattgtt
     2821 ctaaggcttc tgccgataat gactggccaa tcagccgctc aattaaggta gccaaatgcg
     2881 aagcgtctaa ctgattgacc aaggcgtcta tgccttgtct ggtcaaggta tgacggaaca
     2941 attccaaata gaaataacgc cactgaagct cacttcccag ttgtctgcct tgcgagagcg
     3001 ccagatcaaa ttctgataaa agctggttga ggtgagctag ttgtctgttt agagtagctt
     3061 cactaaccat cagttcaacg gataagttgg tgatgttaaa gtgttcatgg cagtacatgt
     3121 aggtcaatat ctgataggca accgattctt tgagaagcag agctaccaaa tcctcccaat
     3181 ggaactggcc attttctttt aagctcagac tgtctttttc taaaagtaat tgacaggcta
     3241 aaccttgttc tcgacacaaa tgatttaggt tgtcaatgta ttttaatagg gtcactttag
     3301 acaagtttga tagcctcatt aggtctttta gccttaacgt ttctttagca tgataaaggg
     3361 tcaccaagag acgatattga gcttttcgtt ctttatccat taagtcttca atcttcatgg
     3421 cggagcctct tttcaaataa tttgagacaa gcttcatgaa gatgataagc caaatcactg
     3481 tgaccttgta aaaagagaaa atcttgaaat gctaagtcac cgtctgtaac tttcagagcc
     3541 agagcctgac ctaattgatt ggcgatgaca agagcattgg ttttggatag gagctgagcg
     3601 cctattaaaa tcccatccgt ttttctagct attaatttca gccaaaagag gtttttagaa
     3661 tccccatttt ggtacaaata gctacacgta tccatataaa gctctgcttc ttcttctgtc
     3721 acaccaacac tcgcgcgata ataaccaaag tgagaacttc ctaatacctt gaccgtctgt
     3781 aatggaggaa taggatatcc agctaaatgc agagccagag cctgacctgt cttcattgct
     3841 tggttaatca aaggtgtata gtaatccatt cccaatatac caaaatacaa tgaaaccatg
     3901 tctccaatgg catagatatt agcctggctg gtctgtaagt tttcattgac caaaatggtc
     3961 ttatccagag cacaagccac ttgtccttgc aaaaggtgtg tatttggcct aaagttaatg
     4021 gctaaaatag ccaaatcacc ttggaaggtg tccccttgct cggtatgagc gacaactttt
     4081 ccctctatct catggatagc cgtaacacgc ttattattaa taaaggtgac atggtgagtg
     4141 gacatttctg ccattacagg ggctatcagt tctgcgtcag tctgatagaa atctggacgt
     4201 tcggctgctt caatcagttt gacccgtttg ccttgtaagg acaaactgta ggcaaggtct
     4261 aagccaatca agccggcacc aataaccaag acctcttggc tagcctcaat cagttttaaa
     4321 ctagcttggc tttcttcata atatttggtt accaaaactt tgttggtctg tgacgtctca
     4381 atataatgag actgggggct agcacccatg gcacaaacaa gcgtttcata accttcttca
     4441 aaaactcgtc cttgactgtc tttgagaagc aaggtgtttg agggagcttc gatagctaa
//
LOCUS       DAUVUY010000109         4444 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.110, whole genome shotgun sequence.
ACCESSION   DAUVUY010000109 DAUVUY010000000
VERSION     DAUVUY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.110"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1336
                     /gene="glnA"
                     /locus_tag="VOX58_001753"
     CDS             <1..1336
                     /gene="glnA"
                     /locus_tag="VOX58_001753"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HES5878966.1"
                     /translation="VADIRHEVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLDKVLS
                     NKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEGKPFA
                     GDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDLAPID
                     LADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLVVKTI
                     AREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAYYFLG
                     GLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGTRLEL
                     RSVDPTANPYLALAVLLEAGLDGIINKIEAPEPIEANIYTMTMEERNEAGIIDLPSTL
                     HNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFISQWEIDHYIHNY"
     gene            complement(1564..3246)
                     /locus_tag="VOX58_001754"
     CDS             complement(1564..3246)
                     /locus_tag="VOX58_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HES5878967.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PNGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(3248..3478)
                     /locus_tag="VOX58_001755"
     CDS             complement(3248..3478)
                     /locus_tag="VOX58_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HES5878968.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            3762..>4444
                     /gene="tsaB"
                     /locus_tag="VOX58_001756"
     CDS             3762..>4444
                     /gene="tsaB"
                     /locus_tag="VOX58_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HES5878969.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHAPLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSH"
BASE COUNT         1378 a          800 c          886 g         1380 t
ORIGIN      
        1 agtagctgac attcgtcatg aagtcaaaga aaaaaatgta acgtttcttc gcttgatgtt
       61 cactgatatc atgggcgtta tgaaaaatgt ggagattcct gcaacgaaag aacagttaga
      121 caaagtattg tctaacaagg ttatgtttga tggttcatct atcgaaggtt ttgtacggat
      181 caatgagtca gatatgtacc tttaccccga tttagacact tggattgttt ttccctgggg
      241 agatgaaaat ggagcagttg caggtttaat ttgtgatatt tatacagcag aaggaaagcc
      301 ttttgcagga gatcctagag gaaatttaaa aagagccctg aaacacatga acgagatcgg
      361 ctacaaatca tttaatcttg ggccagaacc agaatttttc ctttttaaga tggatgataa
      421 aggtaatccg acacttgaag ttaacgataa tggtggttat tttgatttag cgccaattga
      481 cttagcagac aacacgcgcc gtgaaattgt gaatatttta acgaaaatgg gttttgaagt
      541 ggaagctagt catcatgaag tggctgttgg tcaacatgag attgatttta aatatgcaga
      601 tgttttgaaa gcttgtgata atattcaaat ttttaagcta gttgtaaaaa cgattgcccg
      661 tgaacatgga ctttatgcta cttttatggc taaaccaaaa tttggaatag ctggatcagg
      721 gatgcactgt aacatgtctt tgtttgataa ccaaggtaat aatgcttttt atgatgaaac
      781 tgataagcga ggaatgcagc tatcagaaga tgcttattat ttcttgggag gactaatgaa
      841 gcatgcttat aactacactg ctatcactaa ccctacagtg aattcttaca aacgattagt
      901 tccgggttat gaggcacctg tttatgtcgc ttgggctgga agtaatcgtt caccgcttat
      961 ccgtgttcca gcatcacgtg gtatgggaac gcgtttggag ttacgttcgg ttgatccgac
     1021 agctaatcct tatttagcct tggctgttct cttggaagct ggattagatg gtatcattaa
     1081 caaaattgaa gctccagaac ccattgaagc taacatttat accatgacaa tggaagaacg
     1141 aaatgaagca ggcattattg atttgccatc aacgcttcat aatgccttaa aagctcttca
     1201 aaaagatgat gtggtacaaa aggcactagg ttaccatatc tacactaatt tcttagaagc
     1261 aaaacgaatt gaatggtctt cctatgcaac ttttatttct caatgggaaa ttgaccatta
     1321 tattcataat tattagactt atcaggacct gctcaagttg agtgggtctt ttggatgagc
     1381 tattgtattt gagctagcaa gctattatta gctgatcgga caccaattga ttttccatta
     1441 tagaaaacta aaaaccagtc catgttctat gaggactggt ttttagtttg ataattatct
     1501 gaactagaac tgatatgtta aagatataga cagatagtat taagaaatga gtcttatttg
     1561 attttagtgc ttatcaggtg ttaagaccat cggaattata attggttcac gctcagtctt
     1621 ttcatacaag aatggacgaa gagcattgac gatagcaccg ttaactgatt ggatgctagc
     1681 atccttattt tttaaagcaa tacggatagc gttaaagagc acacgttggc tttcgcggat
     1741 caagtcacct gactcacgca tataaatgaa accacggctc agaatatctg gtccagctaa
     1801 aatcatttgt gtgttaaaat caacagttgc aacagctaag acaacaccat cttctgacaa
     1861 atcacggcgg tctcgtaaaa cggctgctcc aatatccccg atgccattac catccacgta
     1921 gatgtcttgg gcattaaagt gaccagcaat acgcgcactg tcactggtta aagctagaac
     1981 gtctccattt tccataataa agatattttc ttttggaata ccaatatcca tcgccagtcc
     2041 tgcatgaact ttttgcatgc gatactcacc atgtactggc atgaaatatt ttggtttaat
     2101 caaactaagc atgagctttt gttcttgctg tcctccatga ccagaagtat gaatattatt
     2161 gacttttcca tgaataacat caacaccagc ttcttggatg gtattgatta atttattgac
     2221 acttgtggta ttaccaggga ttggacttga tgagaaaatc acagtatcac caggctgtaa
     2281 agttacttga cgatgagtgc cattagcgat acgagcaagt gctgccatag actcaccttg
     2341 actacctgta cacataatta aaacttcact agcatgaaga tttttaagtt cgcttggctc
     2401 aatgaaggta ccatttggaa ctttaatata gccaagttca ataccgttaa caattgcttt
     2461 ttccatagaa cgaccaaaaa cggcaatttt acgtcccgtt ttaacagcag cttctgcagc
     2521 ctgttgcaaa cggtagatgt ttgaagcgaa ggaagcaaaa ataatacgac catgaatacc
     2581 ttcgataatt tttaaaatgg attggccaac aactttttca gagttagtaa aggttgggat
     2641 ttcagcatta gttgagtctg ataataagca aaggacacct tcttcaccaa gcgctgccat
     2701 acgttgtaaa tctgcaggat ctcctactgg cgtgaagtca aatttaaaat caccagtaca
     2761 gataatctta ccttgagggg tgtggataac aataccgacc ggttctggaa tggaatgagt
     2821 agtcttgaag aaagtgacgc tcatgttttt aaaagttaac tcggtgttgt gattgatttc
     2881 gtaaacagtt gcttcacgcc aaagaccatg ttcttccaat ttaccacgga taagagcaag
     2941 tgctaaaggt cctgcgtaga tagggatatt agcctgtttg agaagaaatg gaataccacc
     3001 aatgtggtct tcgtgaccat gtgtaataac aagagctttt acccggtcta agttatcgac
     3061 aatgtaagaa tagtctggaa taacgtaatc aattcccaaa agatcatctt ctgggaattt
     3121 gataccggca tcaacgataa tgatctcgtc ttgatactca ataccataag tattcttccc
     3181 aatctctccc agaccaccaa tggcaaacac cccaacttca ttaggtttta aactgatatt
     3241 tgtcatctta gaactccgtt aattcaaaaa caccggtctc tttttcgtaa tcgaggtgtt
     3301 tgtcagaaag tagctcgata aattccacgt tataataggt gttatcttca acgagacgac
     3361 gagccttgat acggccatcg agttcatcag tagcatcaat attaagatag agtgctttag
     3421 tactttcacg acgtgggctt tgatcctttg tttcttgata gaaaacttta taaatcattt
     3481 gttttttatt cctttcgtta tgaatcaatt atccaaacaa taataaatag gtcaaaaggt
     3541 aggtgttcca ttttgacggt tttatatgtt gtcattattg tgtagtagat taattcctga
     3601 ttctgtgcaa ttattataat tataccataa acttagcttt tagtaaaaaa tttaggtaca
     3661 tttatggtat agtctaaatc aaattaagag tcagcctttt tgtttatgat tcttaatttg
     3721 tgttataatt gatactagaa cttgcagtag aaaggcataa aatgaagaca cttgcatttg
     3781 atacctcaaa taaaaccttg tcccttgcta tacttgatga tgagacactt ctagcagata
     3841 tgacccttaa cattcagaaa aatcatagtg ttagccttat gcctgctatt gattttttga
     3901 tgacttgtac tgatcttaaa cctcaagatt tagaaagaat agtggttgca aaaggccctg
     3961 gatcttacac aggtttacga gtggcagttg ctactgcaaa aacgttagcg tacagtttaa
     4021 atattgcatt ggtcgggatt tccagtctat atgctttggc tgcgtctact tgtaaacagt
     4081 atccaaatac tttggtggtg ccattgattg atgctagaag gcaaaatgcg tatgtaggtt
     4141 attatcggca aggaaaatca gtgatgccac aagcccatgc tccactagaa gttattatag
     4201 aacaattagt agaagaaggg cagctgattt ttgttgggga gactgctcct tttgctgagc
     4261 aaattcaaaa gaaactacct caggcgatac tacttccaac cctgccttct gcttacgaat
     4321 gtggtctttt ggggcaaagt ttggcaccag aaaatgtaga cgcctttgtc cctcaatatc
     4381 tcaagagagt ggaagctgaa gaaaactggc tcaaagataa tgagataaaa gatgatagtc
     4441 acta
//
LOCUS       DAUVUY010000110         4382 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.111, whole genome shotgun sequence.
ACCESSION   DAUVUY010000110 DAUVUY010000000
VERSION     DAUVUY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.111"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..515)
                     /locus_tag="VOX58_001757"
     CDS             complement(<1..515)
                     /locus_tag="VOX58_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HES5878970.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVTLT
                     SLWSQRCNLSKA"
     gene            complement(490..1449)
                     /gene="nrdF"
                     /locus_tag="VOX58_001758"
     CDS             complement(490..1449)
                     /gene="nrdF"
                     /locus_tag="VOX58_001758"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HES5878971.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINNIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            complement(1782..3941)
                     /gene="nrdE"
                     /locus_tag="VOX58_001759"
     CDS             complement(1782..3941)
                     /gene="nrdE"
                     /locus_tag="VOX58_001759"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HES5878972.1"
                     /translation="MSLKDIGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     EHQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(3961..4179)
                     /locus_tag="VOX58_001760"
     CDS             complement(3961..4179)
                     /locus_tag="VOX58_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HES5878973.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
BASE COUNT         1407 a          766 c          866 g         1343 t
ORIGIN      
        1 gcttttgaca agttgcatct ttggctccaa agagacgtca aggtaacagc gacttcccgt
       61 gaagctcctt ctcggccgac aggagctccc atagatacgg tcgttaattg gagccaacca
      121 tgccaaaact gtgtccaggg actaaatgag atgtcatctt gaatgatttg ctgaatagat
      181 tgaatgtcct ttcctttttt agccaatagg tgccacccaa gacctgcaag aagtccagca
      241 aagagaagtg aaagagctcg tctttgtggt ggaacgctag caatcatagt gctaaaagaa
      301 ccttgactga atccaaaact gagggactga atcaggtgaa tggcttttgt taagataaaa
      361 gcaacaaggc cagctgttaa acctgttaaa aagaggtaaa aggtcaagag taaaaagtgt
      421 cgtttcataa gactattata acaaaagtta agaaaagaga ctgatagatt atcagtctcg
      481 tgagcactat tagagtccat aattataatc gtcatcagac atagcttcta cagaaccaag
      541 aagatagcca ttccctacct gtgagaaaaa gtcatggttt gatgttcctg ttgaaatacc
      601 attcataaca attgggttaa catcgtttgc agtatctggg aataaaggat cttgtcctaa
      661 attcataaga gctttgttag cattgtagcg taaaaaggtc ataacttcct cagtccaccc
      721 tacgccatca taaagcgttt tggtgtattt ttcttcattt tcatacagct gataaaggag
      781 gtcatacatc caatcacgaa aattctcttg ctcatcttct ggtagttcgt taaaaccaag
      841 ctggaatttg taaccgatat aagtaccatg tacagattca tcacgaataa ttagtttaat
      901 gatttcagca acatttgcta gcttattatt tcccaaatag taaagagggg tgaaaaagcc
      961 agaataaaaa aggaaagttt cgagatatgt tgaggcaact ttcttttgta aggcatctcc
     1021 attagcgtaa atgttattga taatacgtgc tttttcttga aggaactcat tattattagt
     1081 ccactcaaag atctcttcaa tttctttttt ggtgtttaag gttgaaaaga tagacgagta
     1141 tgatttagca tgaactgatt ccataaattg aatattgttt aagacagctt cttcgtgagg
     1201 agtgcgaaca tcagcacgaa tagcttcgac accagtttct gattgcatgg tatcaagtag
     1261 ggttaatcct ccaaaaacct tgccaacaag atctttttct tgaagagaaa gtttgcgcca
     1321 atcgtctaag tcatttgata aagggatacg ggtatcaagc caaaattgtt cggtcaactt
     1381 ttcccaagtt gacttgtcaa tgacgtcttc gatttcattc cagttaattg cttcataata
     1441 tgtagtcata gaaaaatgat ctcctaaaga gtagtaaagt gcgttaagga tatcgagata
     1501 aaccagtaga accgaccaaa gttctaccgg taataataag tttaaataga ttcaaaaggg
     1561 taacgaatga gtcagtgagt ggtttaggcg gaacgcctaa agcgattgtc caagaagtaa
     1621 tagatgcttt agcatcatta gggcatatcg aggaattacg acacggagta aggaaagtcg
     1681 ataatacttt ccttacgtag ttagtgagtg gtttaggcag atcgcctaaa gcgactgccg
     1741 tagaagtaat agatgcttta gcatcattac ttcttaacca attaaatgac acaagattca
     1801 cattggtttg cgcccacttc ttccccatca tccgtaaagg tacggatata gtagatagat
     1861 ttaatgcctt tattgaaagc gtagtttcgt aagatggata aatcacgagt ggtttgtttg
     1921 ctttgtgttt tccactcata aagctccata ggcaactcac tacgaaggaa tagagttaat
     1981 gacaagcctt ggtccacatg ttcggtcgca gcggcataga catcaataac tttgcgcatg
     2041 tccatatcgt aagcagatgt atagtaagga atggtatccg tagacaaacc atttgcagga
     2101 tagtagattt taccaatttt cttttcttga cgttcttcaa ttctttgtgt gattgggtga
     2161 atagaagcag agcagtcatt gatataagaa atagagccat ttggagcaac tgctagacgg
     2221 ttttgatgat aaagaccatc tttttgaacg gcgtcgcgaa gagcctccca atctgaagct
     2281 tgcggaataa agtgatcttt aaagagactt ttgaccaaat cagattttgg aacaaagtgt
     2341 cctgtaacgt atttatcaaa gtaactacca ttagcgtact tagagttctc aaagccaaca
     2401 aaggtagttt ggtgctcacg agcgatgtta ttggattcga ccaaggtcca ataattcagg
     2461 agcataaagt aaatatcagt aaactcgatg gattctggac tgccatattc aatatgatgt
     2521 tgagcaaggt aagaatgtag tcccatagct ccaaggccaa aagtatgagc ttggctattg
     2581 ccatgtttaa tggttggaac agcttcaatg cttgatgaat cagtaacaaa agttagggca
     2641 cgtgtcatgg tcttaataga acggccaaag tctggtgagg tcatcatgtt caggatatta
     2701 gtggaaccta agttacatga aatatcagtt cccatttcta caaactcttg cgcatcattg
     2761 ataaggctag gtgtttgaac ttgtaaaatt tcagaacaca agttgctcat gatgattttt
     2821 ccatcgatag gattagcttt attagctgta tcaatattga tgatataagg gtaaccagat
     2881 tcttgttgta attttgaaat ctctgtttca agatcacgag ctttaatttt agtcttagta
     2941 attttagggt tcgccactaa ctcatcgtac atattggtaa tgtcgagata gttaaaggga
     3001 atgccatatt ctttttcaac attgtaagga ctaaagagat acatgtcctc gtttttacga
     3061 gctaattcgt agaatttatc aggaacggta atccctagtg acaaggtttt aacacgcacc
     3121 ttttcatcgg cattttcttt tttagtagat aagaaagcaa tgatatcagg atgaaaaaca
     3181 tttaggtaaa caacaccagc tccttgacgt tgcccaagtt gatttgaata agaaaaacta
     3241 tcttcaaata atttcataac aggaacaact cctgaggctg caccagcata gcctttgatt
     3301 ggtgcgccag cttcacggag gttagacaag gtaatcccaa cacctccacc aatacgggat
     3361 aattgcaagg cagagttaat ggaacgtccg atagaattca tgtcatcagt tacttgaatc
     3421 aagaaacaag agaccaattc accacgacgg cttcgaccag catttaaaaa ggaaggagta
     3481 gccggttggt aacgttggtt aatcatttca acggctaaat cttttgctaa gtcttcttga
     3541 ccatctgcaa aatacaaagc attaaacaag acacggtctt caaggttttc taaataatgc
     3601 tctccatcat ttgtttttaa ggcgtattgc tggtagaact tgtaggctgc cataaatgat
     3661 ttaaagcgaa aattttctga tttgattatg ctatctaatt cagtaataaa ttcaggacgg
     3721 tatttctgaa taaaagctga ttcaatgtaa tcatttgaga ttaaatactc aattttttcc
     3781 gtaatggaag taaaagacat ggtgtttggc agcacatttt cagcgaaaaa agcttttaaa
     3841 gcttctttgt ctttatgaag tggaatttta ccattaacag gacggttaat ttcattattt
     3901 aggcgaaaat atgaaatatc gccaatatct ttgagactca tattattttt ccttgtctta
     3961 ttaaatcaat tcttttaact ttgcaggttg gaagccagaa aatacgaggt tatctgcttc
     4021 gataacagga gcagatgtga agccaagact tttcacataa tccacttttt caggatgttc
     4081 atcgatatta atttcctgga agttggctcc gtgttgttct aaaaatttct tagtcatttt
     4141 gcactgcatg caattatttt tggaatatac tgtaatcatg tcttgtcctt ctaaaattag
     4201 taatggttcc atcttaaatg gaattactat aatagcatat aaaaaataaa gctaaaaagc
     4261 aagctataaa cttatctaaa acacaacata ttgtgttgaa ataaaaaaca gaaacctaaa
     4321 tattgtgtca atctggattc ttatggttag agctctagcg ttttggtgat agaagctaag
     4381 ca
//
LOCUS       DAUVUY010000111         4236 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.112, whole genome shotgun sequence.
ACCESSION   DAUVUY010000111 DAUVUY010000000
VERSION     DAUVUY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.112"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..884)
                     /locus_tag="VOX58_001761"
     CDS             complement(<1..884)
                     /locus_tag="VOX58_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5878974.1"
                     /translation="MKLKLSVIGLFFCLIAAIGVISISDTQKPVPLPIDGAFSIQGKS
                     SLSSDEVYKKISNLSKTKNVNIYKPIVRNSGQLTYINFKNTNYEQLKPAPITGMYYTL
                     GKIDSSSLKVLTASGLKLVYAAYPWYIGGILQFDGTLRLLLMLSIYLTLLVVLFVVRT
                     RQIKEGVIRHSLGLPIYNLRRDYVISFTFELVMAGLLMIAYSYFWGSGLFTYSSKLFF
                     SLILTNLIIFQIVDIITFILFWLTIRVEKPIEIIKNKAKNSFLFIVWLVIISIIIIVS
                     GIFLQETKASQSRINRQI"
     gene            1552..2721
                     /locus_tag="VOX58_001762"
     CDS             1552..2721
                     /locus_tag="VOX58_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HES5878975.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGQVLTSTEVVSVKKQDDLFYLKSTDQTFTCQKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTISY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            complement(2816..3487)
                     /gene="deoC"
                     /locus_tag="VOX58_001763"
     CDS             complement(2816..3487)
                     /gene="deoC"
                     /locus_tag="VOX58_001763"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HES5878976.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIQNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAKTFIALGASRLGTSRIIKIVKNEAT
                     KTDSY"
     gene            complement(3517..>4236)
                     /locus_tag="VOX58_001764"
     CDS             complement(3517..>4236)
                     /locus_tag="VOX58_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HES5878977.1"
                     /translation="RMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVP
                     IGSILIAKILLPQTEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIA
                     FVGLVSLINMMLSGLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFV
                     SFQQLGHLIKSLDYRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRA
                     MIGGIAVSMLSAFIVGIVTLF"
BASE COUNT         1409 a          838 c          683 g         1306 t
ORIGIN      
        1 cttatctgtc tatttatcct tgattgacta gcctttgttt cttgtaaaaa gattcctgag
       61 acgattatta taatagaaat aattacaagc catacaataa aaagaaaact attttttgct
      121 ttatttttta taatttcaat aggtttctca accctaatag ttagccaaaa taaaataaaa
      181 gtgataatgt caacaatctg gaatattata agattagtta aaattagaga gaaaaataac
      241 ttagaactgt aagtaaagag gccacttccc caaaaatagc tgtaggcaat cattaataaa
      301 ccagccataa ccaattcaaa agtaaaagaa atgacataat ctcttcttaa gttatatata
      361 ggcaatccta aagaatgtct tatcactcct tctttaatct gcctcgttct aacaacaaat
      421 aaaacaacta ataatgttaa gtaaattgat agcatcaata acaaccttaa agtcccatca
      481 aattgtaaaa tcccccctat ataccaagga taggctgcat aaacaagttt caaacctgaa
      541 gctgtaagta cctttaagct acttgaatct attttaccta aggtataata catgcctgtt
      601 atcggtgcag gttttaattg ttcataattt gtatttttaa aattgatata agtcaactga
      661 cctgagttac gaactatagg cttgtaaata ttaacgtttt tcgtttttga tagattacta
      721 atctttttgt aaacttcgtc actagaaagg ctactttttc cttggattga aaaggcacca
      781 tcaattggta aaggtaccgg tttttgagta tcactaatac tgataacacc tatcgcagca
      841 attaagcaaa agaataatcc aatgacagat aattttaatt tcataaacaa cttcctatat
      901 tataaaagac cctataataa ttattgggtc tttagcttgc atataccatt tctaaaaatt
      961 aaagtaattc caatgtgcat tcaaccgaat atcattccaa gctttttttg aacttgacag
     1021 tgcccacccg tattttttga gaatcagatg ccttaatgga tccccaataa ttagttatcg
     1081 aagattttga accaatattg tttggagatt taacataata attagaataa ccatagttgt
     1141 cacttactcc ataattccat tcatcaataa ttggagatgg agctggatgt gctgttgccg
     1201 cactgacaac actagttacg gccgtaaatc ccaaacttaa agagcttaca gcaagcaaag
     1261 ttatcacttt gctattaaat ctttttttat actattctcc tttaataaaa ttatttaatt
     1321 tttaaaaaat ttcaaaataa cttaatgatt acttcattct ttagtaatac aatagcatga
     1381 attaaataaa aaagcaaccg ttttcataaa aagataattt taatcatctg gactattttt
     1441 ctagcttttt gatgtcatgg aggcattgtt tagatacgta aaaaaacggt atgccaggtt
     1501 agttcattta cctgactatt aaaccccccg cttttatgct aaaataaggc tatgactcaa
     1561 tacgatacga ttattattgg aggtggccct gctggtatga tggccgccat ctccagcagc
     1621 tactatggct ataaaactct cttaattgaa aaaaatcgcc gcttaggtaa aaaattagcg
     1681 ggaaccggtg gtggacgctg taatgtcact aatagtggta accttgatga ccttatggca
     1741 ggcattcctg ggaatggccg ctttttatat agtgtttttt cccagtttga caaccatgac
     1801 atcattgcct ttttcgagga aaacggcgtc aaactcaaag aagaagacca tggacggatg
     1861 ttccccacaa cagataaatc aaggacaatc attgatgctc tggagaaaaa aatcaaggct
     1921 cttgggggtc aggtgctaac cagcactgaa gtggtttccg ttaaaaaaca agatgacctc
     1981 ttctacctca aatctacaga ccagactttc acctgccaaa aattgattgt gaccacagga
     2041 ggcaagtcct acccttcaac gggttcaaca ggttttggac atgatattgc acggcatttt
     2101 aaactgaccg tgactgattt agaagctgct gagagccctc tgttaactga ttttcctcac
     2161 aaagtcttgc aaggtatttc cctagacgac gttactatta gttatgacaa acacgtcatc
     2221 acacacgact tactcttcac ccactttggc ctttcaggac ctgcagctct tcgtctgtcc
     2281 tcttttgtga aaggtggaga aattgcagag ctggactttt tgccacactt atcaacagat
     2341 gacttaaccg cttatctgag tgatcaacgt gataaaaaca tcaaaaatgc tcttaaaggc
     2401 ctactgcctg aacgagtagc tgatttttta tcagaagatt accccgaaaa ggtcaaacaa
     2461 ctctctccaa aacaagaaaa ggaattgctt gataaactca aacacctcca gatacccatt
     2521 acaggaaaaa tgtctttggc taaatcattt gtgaccaaag gaggcgtaga tttaaaagaa
     2581 atcaacccta aaaccttgga aagcaagaaa gttcctggcc tgtatttcgc tggagaggtc
     2641 ttagatatta atgctcacac cggagggttt aatattacct cagctctttg ttcaggatgg
     2701 atcgcaggga agtcttcata gctaaaaaag cctattatca ctaagatagt aggcttttta
     2761 actcctatta tatgagtttt ttgttttagt ctcaagctaa atcggtatga ttaagttaat
     2821 agctatcggt ttttgtagct tcgttcttaa caatcttgat gatacggcta gtacccaagc
     2881 gtgaagctcc taaagcaata aatgttttag catcttccaa tgatgagatt ccacctgctg
     2941 ccttaatttt aacaccttcg ccgacatatt ttgccatcac ttcaacatct tcaaatgtag
     3001 cacctgctgt cgaaaaacca gtagaggttt taataaagtc tgcacctgaa cgtgtgacaa
     3061 ctccacaaag ttcgataagt tcttctttag ttaattgaca tgtctcaacg ataactttta
     3121 agatatggtc ttgacatgcg gctttgattt gacgaatttc ttcttcaaca gtatcaaaat
     3181 ccccattttt aacgtctgtc aaattaatga ccatgtcaat ttcatcagca ccattttgaa
     3241 tagcatcttg acattcaaaa accttcgctg cagttgtact atagccattt gggaacccaa
     3301 taacagtaca aatagctaat ttacctgaaa cgtattctgc tgcttttttg acgtaagaag
     3361 ctggaataca tgctgaagct gtttcataag ccatggcatc atctaaaatt gtttggattt
     3421 ctggccacgt tgctgttgtt gctagcaaag tatggtctac cgtttttaaa atatctttta
     3481 cttccacgtt actatccttc tttcttttgt caacgtttag aatagagtta caataccgac
     3541 gataaaggcg ctaagcatac ttacagcaat accaccaatc attgcacgga aaacaagtcg
     3601 agctagggtg ctacgtttct ccgggcaaag aacagcaata cctgaaacac aaatacctaa
     3661 acttgataaa ttagcaaaac cacagagtga aatagttgct accaatgctg tacgataatc
     3721 taaagatttg attaggtgac ccaattgttg gaacgaaaca aactcattta aaatcaactt
     3781 acttccaaga aggtttcctt ctagaagaat gtttttgtgg tcaaatccca taagaaaacc
     3841 aaatggagca aaaacatatg agaagatttg ttctaagcga attcccaatc cacttaacat
     3901 catattaatc aaagaaacta aaccaacaaa ggcaatcaaa ctagccccaa ttgagaaagc
     3961 catttgtgca cctgtgcttg caccctcagc gattgcatca atcacattgg cgttattacc
     4021 tttattatcc atcttaatgt catcaatttt ttgaacaggt tctgtttgag gcaataagat
     4081 tttagcaatg agaatactgc caataggaac cattgttgaa gcaatcaaga gatattccat
     4141 tggaatgcct aatgcaatat agccaccaag aatagaaact gacatacttc ccatacctga
     4201 taccaacaca accattatct cactatcagt catacg
//
LOCUS       DAUVUY010000112         4201 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.113, whole genome shotgun sequence.
ACCESSION   DAUVUY010000112 DAUVUY010000000
VERSION     DAUVUY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.113"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            329..2254
                     /locus_tag="VOX58_001765"
     CDS             329..2254
                     /locus_tag="VOX58_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HES5878978.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRAGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGEEPV
                     DVSTSKKNKIVALETKISRRRALLQLLFGKVIPLVEVGFLLWQLIQFIRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLVLNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFLSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            2320..3159
                     /locus_tag="VOX58_001766"
     CDS             2320..3159
                     /locus_tag="VOX58_001766"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HES5878979.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKRNAQQEPAVTELARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTDYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            3305..4066
                     /gene="mecA"
                     /locus_tag="VOX58_001767"
     CDS             3305..4066
                     /gene="mecA"
                     /locus_tag="VOX58_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HES5878980.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            4073..>4201
                     /locus_tag="VOX58_001768"
     CDS             4073..>4201
                     /locus_tag="VOX58_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HES5878981.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARR"
BASE COUNT         1264 a          710 c          917 g         1310 t
ORIGIN      
        1 tcttgagtcc agttaaaagg agcataggct gcttccattc cgactcttaa atattggttt
       61 gcttgagccc ggctcattcc tccaaacaga agggccaagg cgaccaaaca gctaaggatt
      121 agctttttca tataggtctc ctgtttcaaa aatagtattc agacctattt tacagcaatt
      181 tgcatcctat ttcaatatat tatcagtgaa taggttgaaa tatggaaaaa tattcaaaat
      241 aatgattaaa aataagatga aggacttggt ttaggttttg tcatctcctt aaaatatggt
      301 aaaatgaggg cagaaagagg atgttgccat gaaaaaaatt ttgatgacgc ttgtgctctg
      361 cttcagtctt ttagggatac gaataaaagc agctgatgta gactatagca tcaccaacta
      421 tgaaggtcag ttactgttat caaaagaaaa tactgctcgg tttgagcaaa aagtaaccta
      481 tcaatttgac acttcttata acggccagta tatttcgctg ggccgggcag gtcatttgcc
      541 agcgggcttt gctattgacc aaaagcctaa agttgaggta tatcagaatg gtcaacaggt
      601 acctgttagt caagagttta gtgaccttgg agatggctat cgtttaaaac tttataatgc
      661 tggccaagct ggtgataagg ttgacgttaa ggttatctgg caactgcacc accttttaac
      721 agcttatcaa gacgtggcag agctgaattg gacaccgatt agtgattggg acaaaacatt
      781 agaaaaggtg agtttgaccg tcactacccc aactgatatt caggattcta atctatgggc
      841 tcacagagga tattaccaaa agaaacctca agtgttaaaa gaaggtaaca gtcgctatca
      901 gattaatgcc aaaaatgtct cagggcaatt ggaattgcat gcttattggg ataaaaaagc
      961 attattggga gaagaaccag ttgatgtttc aacgagtaaa aagaataaaa ttgtagcgtt
     1021 ggaaactaag atttccagac gaagggcctt gttacagctg ctatttggca aagtcattcc
     1081 tctggtagag gtaggttttc ttctgtggca gttaatacag tttataagat taaagaaaca
     1141 attcaatcgt tatcatttgg ccaaccatac agatcacagt tacgaagtcc ctgaagatct
     1201 ttcgcctctt gtgttgacac aagcgattta tggacagagt tttgcctatt tatcccctac
     1261 agcatcagaa agccagaaat tattgatccc taaaggagtg acctttgagg cccttgttca
     1321 agctaccttg ttggatctga tcgaccaaaa ggtgctttta ttaacgaaag aagaagggaa
     1381 agcttatctt gaaatcagtc agttagatcg tgtgacagat gaggaagctg ccttcttaga
     1441 tatggctttt ggaaacaagg tgacattgcc ggtggatcaa ttgtttagtc aataccatta
     1501 tgatgctgat accattaaac aattaaagaa aacgtacaaa ggtaaaaagc tcgagcaaga
     1561 agtccgtcaa tcgtctgagc aggtgattaa ggcgatgaaa aaagcgtcgg cagctattac
     1621 aaacaatgtc ctagaaacta tcaaaaagtt aaacttacca gatacttatc gtcaaatgac
     1681 cccggcagag aaacgaaaaa gcaatagtgt ccagggatta ggttgtttgt tgcttgttct
     1741 aaatagtggt ttattgattt acttggctat taaagaaagt gggttagccc tcatttacct
     1801 tgctttaatg gtgctaacga tgtgccttgg cttttacatt agcctgaagt tagatcaata
     1861 caagaaattg ggtattgaaa cacctgaggg gggtgttcgg ttacatcaat ggcagagctt
     1921 taaaaacatg atacgagaca ttgacaaatt tgaggatgtt gctattgaag gtttggttgt
     1981 ttggaaccgt gttttggttt atgccacctt atttggttac gctaagaaag tggaacgcta
     2041 tttgaaagtc catcgtattg ccttacctga ggtttaccaa gctgttcgac cgggtgaatt
     2101 atcaatggta atgtatgcta cgacaccaac ctttttgtct agcttgtctt cagctacaac
     2161 ttcctcaaat ttctcagtct cttccggagg cgggattagt ggtggtggcg gcttctctgg
     2221 cggtggcgga ggaggcggcg gcggtgcctt ttaaaaaagc tgcttggcat taatcacagt
     2281 ttagaaaatc acaataagag tggttaataa ggagaaaaca tgttaattat cgaattgcta
     2341 aaagccattt tctttggcat cattgaggga atcacagaat ggttaccagt ttcaagcact
     2401 ggtcacctca ttttggtgca ggaatttatc cgactaaatc aggataaggc ttttatagaa
     2461 atgtttaata tcgttattca attaggggcg attatcgctg ttatgttgat ttattttgaa
     2521 cgtctcaatc cctttcaacc tgggaaaaca gcaagagaag tacagcttac ctggcaatta
     2581 tggctaaaag tggtgattgc gtgtattcct tctattttaa tagcggttcc tcttgataat
     2641 tggtttgaag cacattttta ttttatggtt ccgattgcca tcgctttaat cgtctacggg
     2701 attgccttta tctggattga aaaacgaaat gctcaacaag aacctgccgt cactgaatta
     2761 gctagaatgt cttacaaaac agctttcttc attggttgtt ttcaagtctt aagtattgtg
     2821 cctggtacta gtcgatcagg ggctaccatc ttaggagcta ttattttagg aactagtcga
     2881 acagtggccg ctgatttcac cttctttttg gccattccta ccatgtttgg atatagtgga
     2941 ttaaaggctg tgaaattttt ccttgatggc catcaccttg attttgcaca agtccttatt
     3001 cttttagtgg ctagtttgac cgcttttgtg gttagtttat tggcgattag gttcttgaca
     3061 gattatgtta agaaacacga tttcaccatt tttgggaaat accgtattgt gttaggaagc
     3121 cttttactta tttatagctt ctttaaattt gttttttaaa cttacatatc gtttaggatg
     3181 ttgcgacaga ccttggggac tgtcgctttt taaatttcct tttggagaag aggagttagt
     3241 cataaattgc gaaattacag catttctagt ataataaaat gactacgcgt gcgaggtatt
     3301 gagaatggaa atgaaacaaa ttagcgagac aacgctaaaa ataacaatta gtatggacga
     3361 tttagaagaa agaggaatgg agttgaaaga tttcttgatt cctcaagaaa aaacagaaga
     3421 gtttttctat tctgtgatgg acgaactcga tcttccagat aattttaaag atagtggtat
     3481 gcttagtttt cgagtgactc cacgcaagga ccgtctagat gtttttgtta ccaaatctga
     3541 gataaataaa gatattaatc ttgaagattt agcagagttt ggtgatatgt cccaaatgac
     3601 tccggaagat tttttcaaga gcttggagca atccatgcgt gaaaagggag atgtcaaagc
     3661 tcatgaaaaa ttggaaaaaa ttgaagagat aatggaagat gttgttgaag ctactctagc
     3721 taatcagtct gaggcggctg atccatcaac taatcatgag tcagaacctt tagactatgt
     3781 ccattatgta ttggattttt caacgattac agaggcagtg gcttttgcta aaacgattga
     3841 tttttcgata gaagcttcag agttatataa ggggagcaac tgctaccata tgactatttt
     3901 attagatgtt cagcaacaac catcttattt tgcgaatgtc atgtatgcga gattgattga
     3961 gcatgcaaac cctggatcaa aaacaagagc ttatttacaa gagcatggct tacaattgat
     4021 gcttgatggt gctgttgagc aattacaaaa gattgagttg ggataaaata gtatgttttc
     4081 attcacaata gattatgtcc ttgttctgat aggggctttg ttaatgtcct tattcttaac
     4141 ccctcttgtt cgctttttag cctttcgtgt gggtgcagta gacaatccca atgccagacg
     4201 g
//
LOCUS       DAUVUY010000113         4190 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.114, whole genome shotgun sequence.
ACCESSION   DAUVUY010000113 DAUVUY010000000
VERSION     DAUVUY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.114"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            125..1096
                     /gene="fabK"
                     /locus_tag="VOX58_001769"
     CDS             125..1096
                     /gene="fabK"
                     /locus_tag="VOX58_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HES5878982.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            1121..2059
                     /gene="fabD"
                     /locus_tag="VOX58_001770"
     CDS             1121..2059
                     /gene="fabD"
                     /locus_tag="VOX58_001770"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HES5878983.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            2049..2783
                     /gene="fabG"
                     /locus_tag="VOX58_001771"
     CDS             2049..2783
                     /gene="fabG"
                     /locus_tag="VOX58_001771"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HES5878984.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            2798..4030
                     /gene="fabF"
                     /locus_tag="VOX58_001772"
     CDS             2798..4030
                     /gene="fabF"
                     /locus_tag="VOX58_001772"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HES5878985.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMVDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            4032..>4190
                     /locus_tag="VOX58_001773"
     CDS             4032..>4190
                     /locus_tag="VOX58_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HES5878986.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVP"
BASE COUNT         1270 a          711 c          974 g         1235 t
ORIGIN      
        1 caataagtat taatcaaacc attagggtgg gattagactc tttttaggat ctagctcgct
       61 ctttcttctt actataatag tttgattatc aaattattct ccttattttt tatagaaagt
      121 tattatgaaa acacgtatta cagaattact taatattgat taccccattt ttcaaggagg
      181 aatggcttgg gttgctgatg gtgatttagc aggtgcagtt tctaatgctg gtggtttagg
      241 cattataggt ggtggcaatg ctcccaaaga agtcgttaaa gctaatattg atcgtgtcaa
      301 agctattact gatagacctt ttggggttaa tatcatgctt ttatctcctt ttgctgatga
      361 tatcgttgat ctggtcattg aagaaggtgt taaagtagta acaacaggcg caggaaatcc
      421 aggaaagtat atggaaagac tgcaccaggc gggtataatc gttgttcctg ttgtcccaag
      481 cgttgcgcta gccaaacgta tggaaaagct tggggtagat gcagtgattg ctgagggtat
      541 ggaagctgga ggacatattg gcaagttaac gactatgtct ttagtaagac aagttgttga
      601 agcggtttcg attcctgtca ttgcggcagg tggtatagct gatggtcatg gtgcagcagc
      661 agcatttatg ttaggagcag aggctgttca aattggaact cgctttgttg ttgctaaaga
      721 atccaatgcc caccaaaatt ttaaagataa aatcttagca gcaaaagata ttgatacggt
      781 gatttctgcg caggttgtgg gccaccctgt ccgctctatt aaaaataaat tgacctcagc
      841 ttacgctaaa gcagaaaaag catttttaat tggtcaaaaa acagctactg atattgaaga
      901 aatgggagca ggatcgcttc gacatgctgt tattgaaggc gatgtagtca atggatctgt
      961 tatggctggc caaattgcag ggcttgtgag aaaagaagaa agctgtgaaa cgattttaaa
     1021 agatatttat tatggtgcag ctcgtgttat tcaaaatgaa gctaagcgct ggcaatctgt
     1081 ttcaatagaa aagtagataa acacatgaag gataatgata atgacaaaga cagccttttt
     1141 atttgccggt caaggtgccc aaaaattagg gatggcaagg gatttttatg ataactttgc
     1201 tattgtaaga aaaacctttg atcaagctag tcaagtattg ggatacgatt tgcgccgttt
     1261 gattgatagt gacgagttaa aacttaatca aactagctat acgcaaccag ctattttgac
     1321 atcatcaatt gctatttacc gtgttctggg cttacatcac gttaaaccgg atatggtagc
     1381 tggtctttcc ttaggagagt actcagcttt ggtagcatca ggggcactct cttttgaaga
     1441 taccctatcc ttagtagcta agagaggccg cttgatggag gaagcagcac cacaaggatc
     1501 tggaaagatg gttgccgtta tgaatacaga cgtgcaagtc atcgaagaag tctgtcaaat
     1561 agctgctaaa catggagtgg ttgctccagc aaactataat actcctagtc aaattgtaat
     1621 tggtggtcag acagatgctg tgaacgtggc agttgaactt ttaaaggaaa ggggagttaa
     1681 gcgtttaatc cctttaaacg tgtcaggtcc tttccacact gctttgttag aaccagctag
     1741 ccgcttgttg gctaaagagt tggaaagata caacttcagt gacttcaaga ttcctttggt
     1801 tggtaatacc gaagctaata ttatggaaaa agatcgtatc ccagaactat tagcccgtca
     1861 agtcatggag cctgttcgtt tttatgacag tgttgcgact ttagtagaaa gtggcataac
     1921 acaattcatt gaggtaggac caggtaaggt tttgacaggt tttgtgaaga aaattgataa
     1981 aaatttacta tgtactagtg ttgaaaacat ggtaagctta cgtttatttt tagatagaga
     2041 ggatattcat ggaaattaaa ggaaaaaata tatttatcac aggctcaacg agaggtatcg
     2101 gtttagcaat ggctcatcaa ttcgccagtt taggagctaa tattgtctta aatggtcgct
     2161 ctgctatctc tgaggagctt gttgctagtt ttactgacta tggtgttaca gttgttacca
     2221 tctctgggga tgtttcagaa gcatcagaag ctaaacgtat ggtcaatgaa gctatcgaaa
     2281 gtttaggttc gattgatgtt ttagttaaca atgctggcat tactaatgat aaattgatgc
     2341 ttaaaatgac ggaagaggat tttgagcgcg tcttgaaaat caatttgacc ggtgctttta
     2401 acatgactca atcagtgtta aaaccaatga tcaaggcccg ccaaggagca attattaatg
     2461 tctctagtgt ggttggttta acaggcaata ttgggcaagc aaactatgct gcttctaagg
     2521 caggaatgat tggttttacc aagtctgttg cccgtgaagt tgctgctcgt aacatttgcg
     2581 tgaatgctat tgctccagga tttatcgagt ccgacatgac aggtgtcctt cctgaaaaaa
     2641 tgcaagagca aatcttaagt caaatcccaa tgaaacgtat tggaaaagct caagaagttg
     2701 ctcacttagc aagcttcttg gtggaacaag attatattac gggacaagtt atcgctattg
     2761 atggcggtat gacaatgcaa taaggagacg tagtgaaatg acatttaaaa gagtagtggt
     2821 aacaggttat ggtctaacat ctccaatcgg gcatgatcct gaaacgtttt ggaacaatct
     2881 gaaagctggc caaattggta ttggtcctat tactaaattt gacacgactg attatgcagt
     2941 caagaatgct gctgaaattc aggattttcc atttgacaaa tactttgtga aaaaagattt
     3001 gaaccgtttc gatagatatt ctctttatgc tctttacgca gcgaaagagg ctattaatca
     3061 tgctgattta aatattgaga tggttgattc cgatcgattt ggcgttattg ttgcttcagg
     3121 tattggtgga attgcggaga ttgaagagca ggtgattcgc ttacatgaaa aagggccaaa
     3181 acgtgtgaag ccaatgacgt tgccaaaagc gctaccaaat atggctgcag gaaatgtcgc
     3241 catgtcttta aaagctcaag gagtatgtaa atcaattaat actgcctgcg cttcttcaaa
     3301 tgatgcgatt ggggatgcct ttcgtgccat taaatttggg actcaagatg tgatgatagt
     3361 tggtgggtca gaggcggcca taaccaagtt tgccattgct ggcttccaaa gtttaacagc
     3421 tctctcaaca actgaagatc caagcagaag ctctattcca tttgataaag accgaaatgg
     3481 ttttatcatg ggagaaggat caggaatgct tgttttagaa agcctagaac atgcgcaaga
     3541 acgcggggcg acaatcttag cagaaattgt gggttatgga aatacctgcg atgcctacca
     3601 tatgacatct ccaaatcctg agggattagg agctcgtaaa gccatccact tagccttaca
     3661 agaagctggt attgaagcca gtgctattaa ttatgtcaat gcgcacggaa cttcaactcc
     3721 agctaatgaa aaaggagaga gtcaagcaat tgtagccgtt cttggtaagg atgttccggt
     3781 gtcttcaaca aaatcgttta cgggtcacct tcttggagca gcaggcgcta ttgaagctat
     3841 tgccactatc gaggctatgc gtcataacta tgttccgatg acggctggga cacaagcttt
     3901 atcagaggat atcgaagcaa atgtcatctt tggagaagga aaagaaacag ctatcaacta
     3961 tgccatttca aatacttttg gatttggtgg tcataatgct gtattagctt tcaaatgttg
     4021 ggaggagtag gttgaatatt caagaaatca aagatttgat ggctcagttt gatacctcaa
     4081 gcctacgtga atttttattc aaaacaaatg aaggtgagct aatctttagt aaaaacgaac
     4141 aacacctcaa tgcatcgaca tcaaatcaag aacatgctgt gcctgtacca
//
LOCUS       DAUVUY010000114         4139 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.115, whole genome shotgun sequence.
ACCESSION   DAUVUY010000114 DAUVUY010000000
VERSION     DAUVUY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.115"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            294..422
                     /locus_tag="VOX58_001774"
     CDS             294..422
                     /locus_tag="VOX58_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HES5878987.1"
                     /translation="MAFGENGPRKKTTFEKVTMVVVILMVLVTVGGLIASALSVLM"
     gene            479..1792
                     /gene="obgE"
                     /locus_tag="VOX58_001775"
     CDS             479..1792
                     /gene="obgE"
                     /locus_tag="VOX58_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HES5878988.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVNEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDESDLVDEEAYYGFAEAEKEFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDLVRIGKFEFEFVD"
     gene            1861..2019
                     /locus_tag="VOX58_001776"
     CDS             1861..2019
                     /locus_tag="VOX58_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5878989.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(2024..2719)
                     /locus_tag="VOX58_001777"
     CDS             complement(2024..2719)
                     /locus_tag="VOX58_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HES5878990.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDASQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            2944..>4139
                     /locus_tag="VOX58_001778"
     CDS             2944..>4139
                     /locus_tag="VOX58_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HES5878991.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSDAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEVIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHL"
BASE COUNT         1285 a          705 c          956 g         1193 t
ORIGIN      
        1 ccatttgccg taactcataa atcttaactt tcgtttcttg attaaatttc ataaaaaaat
       61 agcaccccaa tcgttagatt ttgtgtctaa cttttggggt gcagttcacc agacgaggag
      121 gtttttatct gtaacattat tttgcaaatg ctaatgtatc atattcatat gaaattcaca
      181 agatacaata ctcagtaata gcctacatag cgtaaatatt catctggtat ttgcctaaaa
      241 cgatctttca tgttaagata taagaagaaa aatcaataga tgaggtattt aacgtggcat
      301 ttggagaaaa tggacctcgc aaaaaaacaa cctttgaaaa agtgacaatg gtcgttgtta
      361 tcctaatggt tcttgtaact gttggcgggc ttattgcgag tgccctatca gtattaatgt
      421 aaggaaacgc tgaaaggcgt ttttttaaag taaaagaaag ttagagaata aaaaaagtat
      481 gagtatgttt ttagatacgg caaaaatcag tgtccaagca ggccgtggtg gcgatggtat
      541 ggtagctttt cgtcgtgaaa agtacgtccc aaatggcggt ccttggggag gagacggcgg
      601 taaaggtggc tcagttatct tccgagtgaa cgaaggtttg cgtaccttga tggatttccg
      661 ctataatcgt aaattcaagg cgaaatctgg tgaaaaaggt atgaccaaag gcatgcacgg
      721 ccgtggtgca gaagatttga ttgtttttgt tcctcaaggg acaacggttc gtgatgctga
      781 aactggaaaa gtaattacag acttagtaga acatggtcaa gaagtggtca ttgctaaggg
      841 tggtcgtggt ggccgtggga atatccgttt tgcaactcct cgtaatcctg cgccagagat
      901 tgctgaaaat ggtgagccag gtgaggaacg tcagttagag ttagaactca aaatcttagc
      961 tgacgttggc ttggtcggat tcccatctgt tgggaaatca accttgttaa gtgttgtgtc
     1021 atcagctaaa ccgaaaattg gtgcctatca ttttaccact attgttccta atcttggaat
     1081 ggttcgtaca aagtcaggtg atagctttgc tatggcagat ctgccaggtt tgattgaagg
     1141 tgctagccaa ggggttggtt tgggaactca attcctccgt catatcgagc ggacacgggt
     1201 tattcttcat gtgattgaca tgtctgctag tgagggtcgt gatccttacg aagattatgt
     1261 ttctattaat aacgagctgg aaacctataa tttgcgattg atggagcgcc cacagattat
     1321 tgtggctaat aaaatggaca tgccagaggc tcaagaaaac ttgaaagcct tcaagaaaaa
     1381 attggctgcc caatatgacg agtttgatga tttgccaatg attttcccta tttcaagcct
     1441 agcacatcaa ggcttagaga acttgttaga agcaacggca gaattacttg ctaagacgga
     1501 tgaattctta ttgtatgatg aatctgatct ggtggacgag gaagcttact atggttttgc
     1561 tgaggctgag aaagaatttg agatcacacg tgacgatgac gctacttggg tcttgtctgg
     1621 tgagaagtta gaacgtctct ttgtcatgac caatatggaa cgtgacgaat ccatcatgaa
     1681 atttgcccgt caattgcgcg gtatgggggt tgacgaagcc cttcgtgaac gtggagcaaa
     1741 agacggggat cttgttcgta ttggcaaatt tgaatttgaa tttgtggatt aatgtggtct
     1801 ttagaaatgg cattgccata gcagccttgt gttttatctt taagatggag gtttctgatt
     1861 atgggtgata aaccgatatc ctttaaggat aaggatggaa attttgtatc ggcagcagat
     1921 gtttggaatg ctgagaaatt agaagaattg tttaatcttt taaatcccaa tagacgtttg
     1981 cgcttagaac gtgaaaaatt gaaaaaggat gaggcttagg ccctcatcct tttttgacct
     2041 ttttttcttg gtaacgttgg agcattagcc aactataaaa agtgtaacta tcattaataa
     2101 gaaaggtggc aaagcagatg accacagcat attggctggc atctgtttga gcagcatgca
     2161 tccataagag aatgaggact agatcattga gtccataagc caaagcaaaa taagggcttc
     2221 gcttatagct gagataaacg gctgagaaag aagtggcaat cgataaagtg gagacaagta
     2281 aataggctgt ttgaatagaa gccaagatgc cgtagaaaat aagagtgacc agtatagtaa
     2341 aaacgaaaag acatcttcga tcagtgggtg ttaatcgtga aatagtcact tgtgattttt
     2401 tcccttcaaa aggatggttt aaccaagtaa aaagtgaaaa aatagtcata gggagggtca
     2461 tgaaaagata ggtcattaac tctccatagt aactgtttcg taatgaaaga taggcataga
     2521 taatggaaaa aataatgacg agcccttgac caataggatt agctttagca ctaaaaatga
     2581 gagaggtcac accaatcaaa gaagctatta aagctaaggg agcttgctta ccgaataaaa
     2641 gggcgctaat taaaatggcc aagacagagc ttaaccaaag tgcccactcg gtttttgtaa
     2701 agtagtttaa ataagccatg cccctattca aacataggct aaggcatctg tcaatagggg
     2761 cttggttttg ataagaagta gcagggcagg ttagataggc tctttaagat aataaaatat
     2821 gtcaagcttt aaatcactta ttttccgtga tgataaggaa aaaacagatt ttttaaagcg
     2881 tttacatcac ctgtttttct tcttataata aaaagagaag tgagcaaaga aaggacaaag
     2941 gatatgaaac aaagaaaaca ccgttatcaa tttccagatg gctttttatg gggaagttcc
     3001 acatctgggc ctcaaagcga aggaacggtt cctggagatg gcaaaggacc tagcaactgg
     3061 gattactggt ttagtattga gtcagctaag tttcatcacc aaattggtcc agaaaaaacc
     3121 tcaacctttt atgaaaacta taagggtgac attgcccttt tgaaagaaac aggccacacg
     3181 atttttcgga cctcaatcca gtggtctcga ttgattccag aaggagtcgg ggaggttaat
     3241 ccaaaggcgg tgacatttta ccgagaagta tttcaagaca ttattgctca aggtattaaa
     3301 ttgattgtta acctctatca ttttgacctc ccctatgcct tgcaagaaaa aggtggctgg
     3361 gaaaacaaag ctactgtctg ggcttatgaa acctacgcaa aaacctgttt tgagcttttt
     3421 ggtgatttag taaacacttg gattaccttt aatgaaccga ttgttccagt cgaatgtggc
     3481 tatttaggtt attatcacta cccttgtaaa gtagatgcca aggcagcagt tcaagtggct
     3541 tacaacacac aactggccag ttcactggcg gttaaagctt gtcataagct tcatcccgac
     3601 cacaagatta gtattgtgct taacatgaca cctgcttatc ctcggagtga tgctcctgaa
     3661 gatgtcaagg ctgctagaat cgcagagctt ttccaaacca aatccttcct agatccatca
     3721 gttttgggtg tttacccagc agagttagtt tccattttag aggaagctga tttattacct
     3781 caatatagtg ctgatgaatt agaggttatc aaaaacaata ctgttgattt tttgggagtt
     3841 aattattatc agcctttgcg ggttcaggca cctagtaagt cacagcaaga gggggacccc
     3901 cttatcttag atatttattt tgaaccttat gatatgcctg gtaaaaaagt caatcctcac
     3961 cggggttggg aaatttatga accaggactt tatgatattg cccttaattt aaaagaacac
     4021 tatggtaaca tcgaatggct agtcacggaa aatggtatgg gtgtcgaagg tgaagaagct
     4081 tttttagcag atggccagat acaagatgac taccgcatca cctttatcga ggatcatct
//
LOCUS       DAUVUY010000115         4075 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.116, whole genome shotgun sequence.
ACCESSION   DAUVUY010000115 DAUVUY010000000
VERSION     DAUVUY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.116"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            141..1586
                     /locus_tag="VOX58_001779"
     CDS             141..1586
                     /locus_tag="VOX58_001779"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HES5878992.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            1981..3327
                     /locus_tag="VOX58_001780"
     CDS             1981..3327
                     /locus_tag="VOX58_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HES5878993.1"
                     /translation="MANSEYMFLLLSIIVYYMTKIYIFSFLSDITLPVWKQLTILALA
                     LFFNQFPYLSPLLIDPLLFLVVLRQETKQLFSLKALFLAVAPSVLVDLLSRFMGTIVI
                     PYLFLSSGIYLGHIIFDLLAYLLIFPSFAIINYMIGKDYKMICQSGYSKRSHNFYQTL
                     LIFVLVYYVDIFVILGFTDPFLHFHHSLFVPTPYKLLFLMFILLLVYLLSYFNHSSKE
                     YLKNELRREQQAYMTNLETYGKHLEKLYRDVRAFQSDYLSRIERLGQAIKSESITQIQ
                     DIYAQTVHEANDYWDDKHYNISKLRKINISSIKSLLSAKIISAEKSGIDLNVEVPDNI
                     KETYIPELDLLLLMSIFCDNAIEAALEAQQPHMSIAYFLLDDYQMFVVTNTTKKKVDI
                     NKIFEEGYSSKGSERGIGLSNAQRILKKYPYLSLRTKSFDKEFSQTLTMPKEEVDR"
     gene            3324..>4075
                     /locus_tag="VOX58_001781"
     CDS             3324..>4075
                     /locus_tag="VOX58_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HES5878994.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSEN"
BASE COUNT         1256 a          658 c          780 g         1381 t
ORIGIN      
        1 cgcctaagca agtaaaatat gctataataa gaaagatttt agaagaagtc tgaaaaggtc
       61 ttggcaagct caaatcatca tttgttgctt gtttttcgtg ccacaggttt ctgagtgata
      121 tatgtaacag gagaagaagc atgtctaaac caattcgtgt gcgttatgca ccaagtccaa
      181 cagggctttt acatatcggg aatgcccgca cagccctatt taattacctt tatgcccgtc
      241 gtcacggagg tacttttatc attcgtattg aagatactga ccgtaaacgt catgttgaag
      301 atggtgaacg ttctcaactt gaaaacctaa aatggttagg aatggattgg gatgaaagcc
      361 cagaaacgca tgaaaattac cgccagtctg aacgtctagc actttatcaa caatatattg
      421 atcaattgtt agctgaagga aaagcctata agtcttacgt gactgaggaa gaattggcag
      481 cagagcgtga acgccaagaa gcagctggcg aaacgcctcg ctatatcaat gaatttattg
      541 gtatgtcagc tgacgaaaaa gctaagtaca ttgctgagcg tgaagcagca ggtatcgttc
      601 caacagtccg tcttgctgtt aatgagtccg gtatttacaa atggactgac atggttaaag
      661 gtgatatcga gtttgaaggt ggtaacatcg gtggtgactg ggttattcaa aagaaagatg
      721 gctacccaac ttataacttt gccgttgttg ttgatgatca tgatatgcaa atttctcacg
      781 ttatccgtgg cgatgaccat attgccaata cgccaaaaca attgatggtc tatgaagctc
      841 ttggctggga agcgccagag tttggtcata tgactcttat catcaattct gaaacgggta
      901 aaaaattatc aaaacgcgac accaataccc tccaattcat tgaagattac cgcaaaaaag
      961 gttatatgcc tgaagctgtc tttaacttca tcgcccttct tggctggaat cctggcggag
     1021 aagaggaaat cttctctcgt gagcaactga ttgccctttt tgatgaaaat cgccttagca
     1081 agtcgccagc tgcctttgac cagaagaaaa tggattggat gagcaatgag tatcttaaac
     1141 atgcggattt tgaaacagtt tatgccctct gcaaaccttt ccttgaagag gcaggccgtt
     1201 tgacagaaaa agctgagaag ctagtagaat tatacaaacc tcaattaaaa tctgcagatg
     1261 agattattcc attgactgac cttttctttt ctgatttccc tgaattaaca gaagccgaaa
     1321 aagaagtgat ggcaggtgaa acagtttcga cagtcttgca agctttcaag gctaagttgg
     1381 aagctatgtc agatgaagac ttcaaaccag aaaatatttt cccacaaatt aaggctgttc
     1441 aaaaagaaac aggaatcaaa gggaaaaacc tctttatgcc tattcgtatt gcagtatctg
     1501 gtgaaatgca tggcccagaa ttaccaaata cgatttattt attgggacgt gataaatcca
     1561 ttgagcacat caaaaacatg ctataaggct gtatcgttca cctttatttt taaacgcttt
     1621 aaggagttaa aaagtcacga caacgaaaga ttgaagatta agttcttcag tcttttttat
     1681 cgttatctga gcttgctacc ttggtaaagt aaaaaatagt atagtttatt tctattttgg
     1741 agattggatt tgaaagagag gtggtgagat gcttacgtga taatgacagc aaacggtgag
     1801 gttttgatta gtgtgattta aagaaatggt ttttatgaat caattagtgt acaatatttt
     1861 tttggtatat gctgaaagag actagtttag tattcactta gctaatcaat tgtaatcaaa
     1921 taatattgta atcattgtca taaaatatgc taaaataata aagttggaaa ggagaaaaaa
     1981 atggctaata gcgaatatat gtttttatta ttatcaatta ttgtctatta tatgacaaaa
     2041 atttatattt tttcctttct atctgatatc acattaccag tttggaaaca actaacgatt
     2101 ttggctttag ccctattttt caatcagttc ccgtatttgt cgccattgct aattgatcct
     2161 ctattatttt tagttgtttt aaggcaagaa accaagcaat tattttcttt gaaagctctt
     2221 tttttggctg ttgcacctag tgttttggtg gatttgctat ctcggtttat gggtacaatc
     2281 gttatcccct atcttttttt gtcgagtggt atttatctgg gtcatattat ctttgacttg
     2341 cttgcttatc tgctgatttt tccaagtttt gctattatta attatatgat tggaaaagat
     2401 tacaagatga tatgccaatc cggatattcg aaacgatcgc ataattttta tcaaacattg
     2461 ctgatttttg ttttggttta ttatgtggac atttttgtca ttttaggatt tacggacccc
     2521 tttttacatt ttcatcattc tctttttgtt cccacacctt ataagttatt gtttttgatg
     2581 tttatacttc ttttagttta tcttctatct tattttaatc atagttctaa agaatacctt
     2641 aaaaatgagt tgagaagaga acaacaagct tatatgacta acttagagac ttatgggaaa
     2701 catcttgaga aactctatcg agatgtaaga gcttttcaaa gtgattacct gagccgtata
     2761 gagcgtttag gtcaagccat taagagtgaa tccattactc agattcaaga tatttatgcc
     2821 caaacggttc atgaagctaa cgactactgg gatgataaac actataatat ctcgaagctt
     2881 aggaaaatta atatctcatc gattaagagc ttattgtcag ctaaaattat cagtgcggaa
     2941 aaatcaggta ttgatttgaa tgtggaagtg ccagataata taaaagagac ttacattcct
     3001 gagttggact tactattgtt gatgtctatt ttttgtgata acgccattga ggcagctctt
     3061 gaagcacagc aacctcatat gtcaattgct tactttttgt tagatgacta tcagatgttt
     3121 gttgtgacaa atacgactaa aaaaaaggta gacattaaca agatctttga agaaggctat
     3181 tcttcaaaag gttcagaacg tggtatcggc ctttctaatg cgcagcgtat tctcaaaaag
     3241 tatccttatt tatccttgcg gactaaaagt tttgataagg aatttagcca aaccttgacc
     3301 atgccaaagg aggaggtgga tagatgatgg ttatatacag tatggcttta ttctatgcta
     3361 ccatctttat taacagctgg gtcatttttg caaaggtttc tgctattaaa ctaagctgga
     3421 aacgggttag cgttataggc attagttttg taattgctaa tatgatattt gacaaagtca
     3481 ttttgattga ccaactgttt tttattattg ttagtttgtt aagtgctccg aaaaagaaat
     3541 tatttgagca tatgtttaat ggtttcttta ctatcttgat tgtcgaatta ctgtttcgcg
     3601 tcatcggatc tttcttttta ccagctgtct taggatttag cattggtcaa attaataata
     3661 accttaagct tttagagtta tgttatcttt ttgtattacc aatattttat ctgtttagct
     3721 acattttcag tattgactta agcttaataa ggtttatcag tgaagataag atgaaaaagt
     3781 gggtattttg gatgaatact gccatgtttt cttactactt tttcgcccat tttttggtaa
     3841 ctgtgcaaag cggatttctt gcactatatt tccagtaccg gtctatttta gtatttattt
     3901 atttagctat ttttatttgg gttattgtaa aattagatcg atttgctaaa gatcagctgt
     3961 cacaaaaatt aacacaagca caaaatgaac gtattgcgta tctggaaaat tacaatcaat
     4021 cgattgagca actttaccga gaaatcagaa cagttaaaca tgattctgaa aatat
//
LOCUS       DAUVUY010000116         4060 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.117, whole genome shotgun sequence.
ACCESSION   DAUVUY010000116 DAUVUY010000000
VERSION     DAUVUY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.117"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            293..448
                     /locus_tag="VOX58_001782"
     CDS             293..448
                     /locus_tag="VOX58_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A2 lantipeptide"
                     /protein_id="HES5878995.1"
                     /translation="MSFMKNSKDILTNAIEEVSEKELMEVAGGKKGSGWFATITDDCP
                     NSVFVCC"
     gene            530..2155
                     /locus_tag="VOX58_001783"
     CDS             530..2155
                     /locus_tag="VOX58_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HES5878996.1"
                     /translation="MIKRNELKLEYQQHQFYEYFNSIFDYDILDTSISENIYLLREKT
                     HKLFYSEFENQLFETIMFLSMKTLVLDINHFSKEIENKSEAYEQCIQQIREENGINHF
                     FDRYPYLLKQINKEVGLIEESYSLLFDRFLEDLSEIRSCFNISEPLSNVAFSLGDSHS
                     KKQTVVKIAFKEKSVYYKPKSYHSHSILLELTSLLKSSNIPSFSLPKSLVKADYCWQL
                     GVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLHMENVIVSGGDLYLIDVETFFQRK
                     LNVQNQNFEGITVDTYQRIYETSLSNGLFPVQFEKNSAPNVSGISGKGGKRKKGKYEL
                     INKNRGDMKLVKVDYFQEDGFNIPTLNGKVVEPLDYANEIISGFRECYIFLLSQRSKI
                     KGIVEGFPELKSRALFRNTSDYGKFLQASTNPKYLFSEKKKKNLFSILYEAKHIEHFI
                     VDNEIKDLMNGDIPYFSMDTRGNVYNSVGTLIGNLGDTTSLFDSITILNDERLKFTCE
                     LLEIVLKKPIKHWEREKGKSYQFLSISSEHYLL"
     gene            2157..2690
                     /locus_tag="VOX58_001784"
     CDS             2157..2690
                     /locus_tag="VOX58_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878997.1"
                     /translation="MDKTDFRNISAFVSQDSPMFDGDVMYNISLGRESVSGEQVIETC
                     KRVSIYDDIRSMPMKFHTPLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALD
                     VKTERIIQKNVEALHCTRILVTHRLNTVEKADKILIMDNGKIIDYGNHHYLYKNNKDY
                     CDLYDSYMNKYQEEEVK"
     gene            2687..3424
                     /locus_tag="VOX58_001785"
     CDS             2687..3424
                     /locus_tag="VOX58_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5878998.1"
                     /translation="MKQLVLKDVCKKYPNQLNYALDHINLIVEKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGSIYCADKEISAFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNVDSSKIDDLINNYAKRFNLYEQLKKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEGTMRILSEINKIEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHMEIDKEDCGETFYDVISQALSDRGE"
     gene            3426..>4060
                     /locus_tag="VOX58_001786"
     CDS             3426..>4060
                     /locus_tag="VOX58_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HES5878999.1"
                     /translation="MIWSITKSNIKKNFSLYRIYFLATIGLLSIFIAFLNFISDKIIT
                     EKIGDSGQALVIANGSLVFLTVFLVIFLIYFNNFFVKKRSQELGVLAILGFSKKELTQ
                     LLTLENLVILVLSYLVSLLLGPTLYFLAVLVITHLLELTLEVQWFITANEIIESLGIL
                     VVVFLINVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAIIAITALLLS"
BASE COUNT         1453 a          511 c          741 g         1355 t
ORIGIN      
        1 ttctgtcatt caaactcctg acctgattaa ataagtaaag taaccatttt ctgattagag
       61 attgatgtta taacaagaag cgattagatt gtaaaatagc tgtaaagaac gagttttgtt
      121 ttttatatca gagggctatt tgttataaag tgacctctta tgtgacatct caaaaatctt
      181 actaaaaaat atgaaaaaag tcatttttgc actttttact cattgaaaat gaagacgtat
      241 tatttacaat aaaagtgtag aaaatagtca ctacatactg atagaaagga gaatgagttt
      301 tatgaaaaat tcaaaggata ttttgactaa tgctatcgaa gaagtttctg aaaaagaact
      361 tatggaagta gctggtggta aaaaaggttc cggttggttt gcaactatta ctgatgactg
      421 tccgaactca gtattcgttt gttgttaatt aaaacaatct ggataagcag actaaaattt
      481 cctgtttaga cttagtctag gcaggatttt taagtagtgg taggaaaaaa tgataaaaag
      541 aaatgaatta aagttagaat atcagcaaca tcagttttac gaatacttta actcaatttt
      601 tgattacgac atattagata cttctattag tgaaaatatt tatttgttaa gggaaaagac
      661 acataagtta ttttatagtg agtttgaaaa tcagttattt gaaacgatca tgttcctttc
      721 gatgaaaaca ctagttttag atataaatca tttttccaag gaaattgaaa ataagtcaga
      781 ggcatatgag cagtgtatac agcagataag agaagaaaac ggaataaatc atttttttga
      841 caggtatccg tatttgctaa aacaaattaa taaagaagta ggactgatag aggaatcata
      901 ttctttatta tttgatagat ttttagaaga cttatctgag ataaggtcgt gctttaacat
      961 tagtgagccc ctaagcaatg ttgccttttc acttggtgac agtcactcta aaaagcaaac
     1021 agttgttaaa attgcgttta aagagaaatc tgtttactac aagcctaaaa gttatcattc
     1081 acacagtatc ttgttagaac ttactagttt attgaaatct agtaatatac cttcattcag
     1141 tcttcctaaa agcttagtta aagcagatta ttgttggcaa cttggtgtag cttatacaag
     1201 ttccaataaa gatgaagtag cgaaaattta ctttaagtat ggtgttttag cagcattttc
     1261 tgaaatattt tctattacgg acttacatat ggaaaatgtg attgtttcag gcggagattt
     1321 atatttaatt gacgttgaaa cattttttca aagaaagttg aatgtacaaa atcagaattt
     1381 tgaagggatt actgtagaca cctatcagag aatttatgag acatcacttt caaacggttt
     1441 atttcctgta caatttgaga aaaacagcgc tcctaacgtt tctggaataa gtggaaaagg
     1501 tggaaaaaga aaaaaaggaa aatatgaact gataaataaa aatcgtggag atatgaagtt
     1561 agtaaaagtt gactattttc aagaagatgg gttcaatatt cccacattaa atgggaaagt
     1621 ggtagagcct cttgactatg ctaatgagat catttcagga tttagagagt gctacatatt
     1681 tcttctatca caacgttcaa agattaaagg aatagtagaa ggttttccag aattgaaaag
     1741 cagagcccta tttcgaaata cttccgatta tggaaaattt ttgcaagctt ctacgaatcc
     1801 aaagtatctt ttctcagaaa aaaagaagaa gaatttattt tcgatactat atgaggcaaa
     1861 acatattgaa cattttattg ttgataatga aattaaagat ttgatgaatg gtgatattcc
     1921 ttacttttca atggatacca gaggaaacgt ctataattct gtagggacat taattggaaa
     1981 tctaggagat acaacctcgt tatttgatag tataactatt ttgaacgatg aaagattgaa
     2041 gttcacttgt gaattgcttg aaatagtctt gaaaaaaccg attaagcatt gggagaggga
     2101 aaagggcaaa tcttatcagt ttttatcaat ctcctcagaa cattaccttc tctaaaattg
     2161 ataaaactga ttttaggaat atatcggctt ttgtatctca agattctccc atgtttgatg
     2221 gggatgtgat gtataacatt tcgctaggga gagaatctgt ttcaggagaa caggttattg
     2281 aaacttgtaa aagggtatca atatatgatg atatcaggag tatgccaatg aagtttcata
     2341 ccccactttt tcgagataat ccatcactat ctggggggca aaaacaacga atttctttag
     2401 caagagaact tgtaaccacc cctagaatct tagttcttga cgaacctaca tcagctttag
     2461 atgtaaaaac tgaaagaata atccaaaaaa atgttgaggc tttacattgt acgaggattt
     2521 tggttaccca tagacttaat acagttgaaa aagctgataa gattttaata atggataatg
     2581 gcaaaattat tgactatgga aaccatcatt atttgtacaa aaataataag gattattgtg
     2641 acttatatga ctcgtatatg aataaatatc aggaggaaga ggtaaaatga aacaattagt
     2701 attaaaagat gtttgcaaaa aatatcccaa tcaactgaat tatgctctag accatatcaa
     2761 tttgattgtg gaaaaaggag aatttgtagc tgtcatgggg cgtagtggta gtggaaaaac
     2821 aacactttta aatgttactt caattattga taaaattgat agtgggagta tttattgtgc
     2881 agacaaagaa attagtgcat tttctgataa cgaggcaact agttttagaa aaaatgatat
     2941 tggttttgtt tttcaagatt atatgctgtt agacagtcta accattaggg agaatatttc
     3001 tgttgctcta tctttaaaaa atgttgattc atcaaaaatt gatgatttga taaataacta
     3061 tgcaaaaaga tttaatttat atgagcagtt gaaaaaatat ccgtatcaac tttctggagg
     3121 tcaaagacag agggtcagta ttatcagagc aatcattaaa gaaccagaga ttatttttgc
     3181 tgacgaacca actggagcgc ttgacttaaa atcttctgaa ggaacaatga ggattttgag
     3241 tgaaatcaat aaaatagaaa aggtaactat tttaatggtt actcatgacg ttttatcagc
     3301 ttcttatgcg gatagagttg tacttctcaa agatggtaaa cttcacatgg agattgataa
     3361 agaggattgt ggagagacgt tttatgatgt aattagtcaa gccttatccg atagaggaga
     3421 gtaatatgat ttggtccatt acaaaatcta acatcaaaaa aaacttttcg ttatatcgta
     3481 tctattttct agctacgatt ggtttattaa gtatttttat agcttttcta aattttatct
     3541 cagataaaat cattacagaa aaaattgggg atagtggtca agctctagtt attgctaacg
     3601 ggtcattagt ttttttgact gtatttttgg tgatattttt aatttatttc aataatttct
     3661 ttgtaaaaaa acgtagtcaa gagcttggag tcttagcaat actagggttt tcaaaaaaag
     3721 aattaacaca attactgact ttagaaaatc ttgttattct agttttaagt tacttggtaa
     3781 gtttactatt gggaccgacc ttatattttt tagcggtact ggtaattact catctattgg
     3841 aattaacatt ggaagttcag tggtttatta cagctaatga gattatagag tctttaggaa
     3901 tattagtcgt agtttttctg attaatgtca tcacaaatgg acttatcatt agtaaacagt
     3961 ctttgattga atttgttaat ttctcaagaa aggctgagaa aaaaattaag ataagaaaag
     4021 tcagagctat tattgctatc actgcattgc tattgtcata
//
LOCUS       DAUVUY010000117         4044 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.118, whole genome shotgun sequence.
ACCESSION   DAUVUY010000117 DAUVUY010000000
VERSION     DAUVUY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.118"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..322)
                     /gene="prs"
                     /locus_tag="VOX58_001787"
     CDS             complement(<1..322)
                     /gene="prs"
                     /locus_tag="VOX58_001787"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HES5879000.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMDLQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSRE"
     gene            complement(575..1771)
                     /locus_tag="VOX58_001788"
     CDS             complement(575..1771)
                     /locus_tag="VOX58_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HES5879001.1"
                     /translation="MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTE
                     QKAAQNQVSALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPAAPSAGPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYR
                     TGSTPMVGAIAVWNDGGYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGV
                     TFIYPH"
     gene            2193..2474
                     /locus_tag="VOX58_001789"
     CDS             2193..2474
                     /locus_tag="VOX58_001789"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HES5879002.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(2951..3038)
                     /locus_tag="VOX58_001790"
     tRNA            complement(2951..3038)
                     /locus_tag="VOX58_001790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3002..3004),aa:Ser,seq:gct)
     gene            complement(3048..3121)
                     /locus_tag="VOX58_001791"
     tRNA            complement(3048..3121)
                     /locus_tag="VOX58_001791"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3085..3087),aa:Ile,seq:gat)
     gene            complement(3156..3226)
                     /locus_tag="VOX58_001792"
     tRNA            complement(3156..3226)
                     /locus_tag="VOX58_001792"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3192..3194),aa:Gly,seq:tcc)
     gene            complement(3239..3311)
                     /locus_tag="VOX58_001793"
     tRNA            complement(3239..3311)
                     /locus_tag="VOX58_001793"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3276..3278),aa:Phe,seq:gaa)
     gene            complement(3332..3405)
                     /locus_tag="VOX58_001794"
     tRNA            complement(3332..3405)
                     /locus_tag="VOX58_001794"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3369..3371),aa:Met,seq:cat)
     gene            complement(3415..3504)
                     /locus_tag="VOX58_001795"
     tRNA            complement(3415..3504)
                     /locus_tag="VOX58_001795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3466..3468),aa:Ser,seq:tga)
     gene            complement(3527..3600)
                     /locus_tag="VOX58_001796"
     tRNA            complement(3527..3600)
                     /locus_tag="VOX58_001796"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3564..3566),aa:Ile,seq:cat)
     gene            complement(3621..3694)
                     /locus_tag="VOX58_001797"
     tRNA            complement(3621..3694)
                     /locus_tag="VOX58_001797"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Met,seq:cat)
     gene            complement(3700..3773)
                     /locus_tag="VOX58_001798"
     tRNA            complement(3700..3773)
                     /locus_tag="VOX58_001798"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3737..3739),aa:Pro,seq:tgg)
     gene            complement(3780..3853)
                     /locus_tag="VOX58_001799"
     tRNA            complement(3780..3853)
                     /locus_tag="VOX58_001799"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3817..3819),aa:Arg,seq:acg)
     gene            complement(3870..3954)
                     /locus_tag="VOX58_001800"
     tRNA            complement(3870..3954)
                     /locus_tag="VOX58_001800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3918..3920),aa:Leu,seq:taa)
     gene            complement(3963..4034)
                     /locus_tag="VOX58_001801"
     tRNA            complement(3963..4034)
                     /locus_tag="VOX58_001801"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4000..4002),aa:Gly,seq:gcc)
BASE COUNT         1255 a          889 c          686 g         1214 t
ORIGIN      
        1 gttcacgaga acgcgcttta cggtcttggc gtgcatagcc gtagtaaggc atcacaactg
       61 aaattttttc agcactagca cgctttaaag catcgaccat aatcaaaatt tccattaaat
      121 tgtcattgac aggtgagcta gttgattgca aaataaagac atgatgacca cgaattgatt
      181 cttcaatatt tacttggatt tcaccatctg aaaactggcg aaccgtagac tttccaagtt
      241 gaagatccat tgcagacgca actttttctg ctaattcctt gtttgacgat aaagcaaaca
      301 attttaaatc agaataagac attttttcct ccaaagtttt aatcctatac cattttaacg
      361 cttttcaagt gaattatcaa atgaaattga aaaaccgtta aagaatgtat taaatatacc
      421 ttttatcgac tttatatact tctcttatat tatacataag cgattggcca aacaactctc
      481 ttataaatta actatatcgt caatactctc agaactattc cacaatagga gtaatcaaaa
      541 aggttagaac aactgttcta acctaaataa tatgttagtg tggataaata aatgtaacac
      601 ctgttggatt aaaccagcca cggtggtcag caatgtactg tctaccactg tagttagact
      661 ccatcacacg aatacttgag gcactttgaa cctcaactac aacggcgaca tgtccataac
      721 caccatcgtt ccaaacggca atcgcaccta ccatcggcgt tgaaccagta cgataaccag
      781 ctgcttgagc actataagcc cattgaccac catttcccca attatttcct gcccaaggag
      841 ctaaagattt agctccccat gtgcattgtc caactggata agtattagaa gaatcatatg
      901 atgtttgtgg tcctgcagaa ggagctgccg gagccgtgag tgcagctggt tcaatagcat
      961 tactactttg cgctgccgga gtagcttgtg gtgctggagt aatagcagat tttgctgctt
     1021 caacagatgc tgcttgttgt gcagcctgtt cttgtgcctt aactttagca gcctgttctt
     1081 gtgctaaggc ttcagcagca gctttttctg cagcttcttt ttgagctacc aaattagctt
     1141 tatcttcagt agcagatgct aattggagag ctaaatttgc agttgcagct accaaattag
     1201 cttgttgagt acgtaatgta ttttggtttt cttcagccat tgccatatta gcggcaatgg
     1261 tattaatagc tgtttggtta gcagcttgct tttcttcaag ggaaacttta tcagcttttt
     1321 gttgttctaa caatttagcg ttagcagaga cagctctatt aattgctact aaacggttta
     1381 caacatcaga aattgattta gaattcaaaa gtgcattaat ataaccagaa gtttcattgt
     1441 ttttataagc actacgagct tgatttttta atttttcatt acgagcaaca atttgacttg
     1501 ttagagcctt aatttcttgc tcaaatcgct ttgaaagcgc ctcaagttct gtatttcttg
     1561 cggtcagttt atcttgttca gattgtagtg aacttacttg agcctgtaac gctgaaactt
     1621 gattctgtgc agctttttgc tctgtagtca gatttgagat aatagaatct tgcttagcaa
     1681 tcttagtact taaatcctcc gctcctacag ttgtagctgc tccgagggta acaccactta
     1741 caagaactgc tgataaaatt ctttttttca taataaaaaa actactcctt ttcgataaga
     1801 catcttatat tttaccaaaa aaaaaagtat ttaatattac gcaattgtta cagttatatg
     1861 taataaacaa taaaaatatt ttagggatta tcttataaaa gtaaagcagc caaaaagata
     1921 tatctttcat ttaaaataga atacagtaaa atcaatttaa atacataaaa ttgtataaaa
     1981 ttacacaaaa agtttgaata ttatatctta ataacgctat aagtaataca aaattgttta
     2041 tcttcactca gacaccttat ccttcttaga atgaattttt agttacttat ccacaggtta
     2101 ataacttctt ttcgcctaaa atattatcca caatagtgac attttctatt atcagttata
     2161 gtataatttc attaggaatc taaaacctta gcatcacggt tttaaattat gtaaatgctg
     2221 gggacgcaaa aagcattcat aatcatctct ttcattatac cagacaggtg agaaggcatg
     2281 tcaaagttat taccataggc atactcagtg cccactacta tgagattact atgctttttc
     2341 tacaggctaa aattatcctc gattgttttc atatccccca acgtcttaga caagctataa
     2401 ctcgctttcg tatccgaatc ataaatactt ctgctataaa acctatcgcg tatagaactc
     2461 tcaaacttta ctgaaaactc atacagcatg attcacggaa gctcatcaat aatagcttta
     2521 ttgcccaatt tttcgtacgc ccataactaa catctagaaa agaaaagctc ttactcacaa
     2581 gatctctgaa tttcaataat tattcctaac tctatcaact cttgctcttc ccatttttca
     2641 gacaattttt aaaatttttc taaaagaaaa gaaaacaaga ttagcaatat acttaaactc
     2701 aactactcta aggccaaatt agaaacagtc aacaacctta tcaaagccat caagttaaac
     2761 gttttactcc tctacagtag tcaaagagcc tatcatttta gttatcgaaa taatgaacga
     2821 tttaaatatt ctatttagtt ggttttgaag attacaaaaa aaagacgata accgtcttct
     2881 ttccccctta tttataattc atcctttaat atgtttcatt taacatatta accaaaatat
     2941 ctttacaaaa cggagaataa gggattcgaa cccttgcgcc agttacccga cctaacgatt
     3001 tagcaaaccg tcctcttcag cctcttgagt aattctccaa atatatatgg gcacgagtgg
     3061 actcgaacca ccgacctcac gcttatcagg cgtgcgctct aaccacctga gctacgcgcc
     3121 caagctttta ttgatatagc ttgggaaaac tataaagcgg gtgacgagaa tcgaactcgc
     3181 gacaacagct tggaaggctg tagttttacc actaaactac acccgcaata tgataacatg
     3241 gcgcgagacg gaatcgaacc gccgacacat ggagcttcaa tccattgctc taccaactga
     3301 gctaccgagc caacaactac atacttatgt attgcgggag caggatttga acctacggcc
     3361 ttcgggttat gagcccgacg agctacctaa ctgctccatc ccgcgatagt taaaaggagg
     3421 atgtgggatt cgaacccacg cacgctttta cacgcctgac ggttttcaag accgttccct
     3481 tcagccggac ttgggtaatc ctccaaaata atgattatct atgatatgga ccttgtagga
     3541 ctcgaaccta cgaccgctcg gttatgagcc gagtgctcta accagttgag ctaaaggtcc
     3601 caagtcatcc ataaaaataa tagcggcgaa ggggatcgaa cccccgacct cccgggtatg
     3661 aaccggacgc tctagccagc tgagctacac cgccattaat cgggaagaca ggattcgaac
     3721 ctgcgacacc ttggtcccaa accaagtact ctaccaagct gagctacttc ccgaactgat
     3781 gcaccctaga ggagtcgaac ctctaaccgc ctgattcgta gtcaggtact ctatccagtt
     3841 gagctaaggg tgctatttca aaaaattaat gccgaggacc ggaatcgaac cggtacgatg
     3901 taaaccatcg caggatttta agtcctgtgc gtctgccagt tccgccaccc cggctcctca
     3961 aaagcgaacg acgggattcg aacccgcgac ccccaccttg gcaaggtgat gttctaccac
     4021 tgaactacgt tcgcaactaa tctc
//
LOCUS       DAUVUY010000118         3982 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.119, whole genome shotgun sequence.
ACCESSION   DAUVUY010000118 DAUVUY010000000
VERSION     DAUVUY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.119"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..384
                     /locus_tag="VOX58_001802"
     CDS             <1..384
                     /locus_tag="VOX58_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HES5879003.1"
                     /translation="HGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPDIML
                     FDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQFLED
                     GTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(532..684)
                     /locus_tag="VOX58_001803"
     CDS             complement(532..684)
                     /locus_tag="VOX58_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879004.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(681..2060)
                     /locus_tag="VOX58_001804"
     CDS             complement(681..2060)
                     /locus_tag="VOX58_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HES5879005.1"
                     /translation="MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGIFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(2238..2687)
                     /locus_tag="VOX58_001805"
     CDS             complement(2238..2687)
                     /locus_tag="VOX58_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HES5879006.1"
                     /translation="MIISAEGDVSVHHCFEAMLISLQEDYRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YHPLDIFQKANYFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(2684..3019)
                     /locus_tag="VOX58_001806"
     CDS             complement(2684..3019)
                     /locus_tag="VOX58_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HES5879007.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(3022..3333)
                     /locus_tag="VOX58_001807"
     CDS             complement(3022..3333)
                     /locus_tag="VOX58_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HES5879008.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(3356..>3982)
                     /locus_tag="VOX58_001808"
     CDS             complement(3356..>3982)
                     /locus_tag="VOX58_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HES5879009.1"
                     /translation="IISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKE
                     VSLPSQMSLAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFY
                     HQMVLRENYSPVIYGEVLAFPHPANPMTYSEQVVVAICREPLEWDKAHQAVHFVFLLS
                     PSKGHNHRLKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
BASE COUNT         1241 a          839 c          766 g         1136 t
ORIGIN      
        1 cacggcatgg cacttctaga aaaagtcgga ctttctgata aggccgacgc cttcccagga
       61 agtttatccg gcgggcaaaa acagcgtgtc gccatcgcca gaagtttagc aatgaaccca
      121 gatattatgc tctttgatga accaacttct gctcttgacc ccgaaatggt tggggacgtt
      181 ttgaacgtta tgaaagactt ggcagaacaa ggcatgacca tgttgattgt tactcacgaa
      241 atggggtttg ctcgtcaagt agctaaccgc gttatcttta cagatggtgg tcaatttttg
      301 gaagatggaa ctcctgaaga aatttttgac catcctaagc accctcgtct cattgaattt
      361 ttagacaagg tcttaaacgt ctaatagagc agacaccata ctaacttcca aaaaataata
      421 aggcaaggaa acaggtttcc ttgccttttt tggttactgt tattattcaa accagcaaaa
      481 tagggatact cttatgactc tctacttcat aagagtgtcc cctatgcttt tttatgggcg
      541 tttatgttta gtgaggtaat aacctaatcc cgtaagagct agtcccgtca ctatgtgtaa
      601 gtatttgtta ccaaccatcg gactaatggc aatgagaaaa aagccaacag ccattaaaaa
      661 agttgctcca tttttttctg tcatgcaagg tcactcgctt tttctttatt ggccaaaatg
      721 acaaatggtg cccagataag gaaggcaaca aagaggttaa acagtgaaac caaacctgcc
      781 ataatattac ctccagtagc taggtaagca taaagtccaa caggggtaat ccaaggtact
      841 gataagaata ccggtggaat caaacctatt gcagtagcaa aataagctat gctagcacaa
      901 attggtggca caattagcca tggaatcaca aaggttggat tcaaaacaat tggcatccca
      961 aatgtaattg gttcgttgat attgaaaata cccattggtg cggacaattt ggcaatggtt
     1021 ttatgctctt ctcgttttga gaatatgaag atagcaataa tcaatgccaa ggttactcct
     1081 gaaccaccca tttgagcata agcatcaaag gacccgcgtg tccaaaggta tggtaaatta
     1141 gcagcgctat gcgctgtatt gtaagcagct gtattttctg ttaaagctac catataaata
     1201 ccatccatca ctggggctaa gacattatga ccatgcaagc cgaagaacca gagcagttga
     1261 actaagaagg ttaacaagat aactgaacca ataccctgtg ataagcctag caatggtaat
     1321 tggatatagg ttgaaatgac atcacttagg gaagaacctg ttaatgcgaa gatgaggtag
     1381 gcgaaaattg ccgaagcgta aatcgcaact gttcctggaa taatggcagc aaaggcttta
     1441 ttaaccgcag gtggtacatt atctggtaag gtaatggtaa tattacgttt ggtcaaagcc
     1501 gcatacacaa aacttgataa aaagccaata attaaagctg tgaacaagcc tgtggcaccg
     1561 acatacttta gagctattgc tccccattga ctagtttcaa tacttgaagc tccacccgca
     1621 gtaacgactg aaagccccat ttctttcaaa ttgctaatta ggttagcaga ggcattttca
     1681 agtggtgtcg aaatggttaa agtctgaggt agggtggcaa taaatgagcc aaaagaaatc
     1741 aatcctcccg ccaatggatt aatcttatgc ataacagcta aattgtaacc gaaagcaaag
     1801 gcaaaaaaga gcgccatgat accaattgtc ccaaaataaa catagccatt gatgtcgata
     1861 acaggttgca tagcttttgc aaaccctgtc catcccatat tgtttggaat atcgcgtaaa
     1921 aaaacgttca ataagacagc aactgaacct gccatggtaa taggcatcat tgaaataaag
     1981 gaatcacgaa tagctactaa atgccgttgt gacccaattt ttcctgaaat ctccataaac
     2041 ttatcagaaa aatgcgacat aaatcttctc ctctttttat ttttttaatg taacaaaggt
     2101 aaccttaaaa gcagcggtgt ctgcctaaca caattatctc aaaatcatcc ttgttaaacg
     2161 tgatgcttag agataaccgg taatcggctc ttatccaaat gaacccttct ctctcattca
     2221 aggtctatga ataatcttca caaagacgat ggttgctgca actgtttagc atattggata
     2281 agggctgcta actgggcttg agattggcgt tctaggcttt ttttgaacag tttgccaaca
     2341 ataaaataat ttgctttttg aaaaatatct agaggatgat agtcataaga ataggtaatt
     2401 ctagtccgat tctcccccag agcctctaat cgataagcca attcctttat cccatgattg
     2461 gaactaaaac aagcagtata taattgcgga gcttctaggg ccgtaataag caccctcact
     2521 gtacgctctt tgtatttacc ataatgttta acatagttta tccccgcttt gatatcatct
     2581 cttgacaact gtctcaaggt attttgtcga taatcttctt ggagcgatat cagcatggct
     2641 tcaaaacaat gatggacgga aacatctcct tctgctgaaa tgatcatgac tcacctcttt
     2701 gtattgctga cattttgtcg taaacggtaa ccaattcctt ggctaaatca ataaaggcta
     2761 aggcagtcat catgtgatct tgcccatgca ccatcaataa agacaattca accgcatcac
     2821 cagctgcttc ttgggacagc aaatgtgtct gcgatttatg ggcaatattt aacgagtttg
     2881 tcgcttcttg aagtaagtgc tgagcctcct caaactgccc atctcgtgct gcatgaatag
     2941 cctctacggc cttgcccttg gcttcgcctc catacatgat taagcccata atagcttcta
     3001 agttggatgg ttccgtcatg cctactcctc catcatcgct agggctgttt caaggacttt
     3061 ttcaccgttc ataagaccat aatcagccat gttaatggca tctactttaa tatctggttc
     3121 aaacttatct ttaaaatcct ttagtaagaa tttgacttgt ggacctaaca agagaacgtc
     3181 aatgctatta gcagccactt cgttgtcagc ttcactcaca ggaactgccc aaatagaaac
     3241 ttcaaggtca cgcgcttggg ctgctttttg catcttagtc accaacatac tagttgacat
     3301 accggcatta caaactaaca taatagattt cataaggttt ctccttttaa tcgttttaat
     3361 catgaataag tggggtaaac actgtcatga atttggaata atttggttct tctagtaacg
     3421 cttgctgaag ctccacctga ttgacaaaag atgccaaacc tggtgataca tattttaacc
     3481 tatgattatg ccctttagac ggggataata gaaagacaaa atgaacagct tgatgcgcct
     3541 tgtcccactc aagaggctct cggcaaatag ccactactac ctgttcagaa taagtcatgg
     3601 gattagcagg atgcggaaag gcaagcactt ctccatagat gactgggctg taattttccc
     3661 tcagtaccat ttgatgataa aagtcctcta caaacgtttc tgttcccgcc tcgtcaagac
     3721 aagcaatcat ccgtaaaagc aagtcttcct ttgagatttt ctcgtctaaa taaagaaaac
     3781 gattagggct aaaaacacct gttagaagct gctctgcttt tgctaaactc atctgtgacg
     3841 gtaaactgac ctctttcttg ataccttctt gttctccaat aaattgtcga atagtttcaa
     3901 tatcctgatt tgacaaaaaa acactgacag taatcactgg tgttaaaaac atcagattgg
     3961 caaggctgat agaggaaata at
//
LOCUS       DAUVUY010000119         3920 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.120, whole genome shotgun sequence.
ACCESSION   DAUVUY010000119 DAUVUY010000000
VERSION     DAUVUY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.120"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            170..1051
                     /locus_tag="VOX58_001809"
     CDS             170..1051
                     /locus_tag="VOX58_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HES5879010.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            1433..1621
                     /locus_tag="VOX58_001810"
     CDS             1433..1621
                     /locus_tag="VOX58_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HES5879011.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            1773..2138
                     /locus_tag="VOX58_001811"
     CDS             1773..2138
                     /locus_tag="VOX58_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HES5879012.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            2138..3802
                     /locus_tag="VOX58_001812"
     CDS             2138..3802
                     /locus_tag="VOX58_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HES5879013.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKNKA
                     EVKEFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSDPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
BASE COUNT         1256 a          667 c          875 g         1122 t
ORIGIN      
        1 ttggttaaat gaaggataag aaggcataga tttactggag aaaacttttg ataatctgct
       61 tttggaaaac gctttaagct tgtgaaagac tagaaattta tggaaaattg tgctagaatg
      121 aacaatgtaa acggggagtt atcccgaaaa ataagaggag gccttacaaa tggcaatcgt
      181 ttcagcagaa aaatttgtcc aagcagctcg tgaaaacgga tatgctgttg gtggatttaa
      241 cacaaacaac ttagagtgga ctcaagcaat cttgcgtgca gcagaagcta aacaagctcc
      301 agttcttatc caaacatcaa tgggtgcagc aaaatacatg ggtggttata aagtatgtca
      361 atctcttatt actaaccttg ttgagtcaat gggtatcact gttcctgttg ctatccacct
      421 tgatcatggt cactacgaag atgctcttga gtgtatcgaa gtaggttaca cttcaatcat
      481 gtttgacggt tcacaccttc cagttgaaga aaaccttgct aaaactgcag aagttgttaa
      541 aattgcacat gctaaaggcg tttcagttga agctgaagtt ggtacaatcg gtggtgaaga
      601 agacggtatc atcggtaaag gtgagctcgc tccaatcgaa gatgctaaag caatggttga
      661 aactggaatt gacttcttgg cagctggtat cggtaacatc cacggtccat acccagaaaa
      721 ctgggaaggt cttgctcttg accacttaga aaaattaaca gcagctgtac caggtttccc
      781 aatcgtattg cacggtggtt ctggtattcc tgacgatcaa atcaaagaag caatccgtct
      841 tggtgttgct aaagttaacg ttaacactga aagtcaaatc gctttctcta acgcaactcg
      901 tgaatttgct cgtaactacg aagctaacga agcagaatat gatggtaaaa aattatttga
      961 cccacgtaaa ttcttggctc caggtatgaa agctgttcaa ggtgctgttg aagaacgtat
     1021 cgatgttttc ggatcagcta acaaagcata atttagagta ttaaataccc taaagccctc
     1081 ttgagggctt ttttagatac aattgacacc ctaaaatgga taccagtttc tgtgaaacaa
     1141 tctttgattg tgaacaaaca aagcataatt tagagtatta aaatctggac cctgctatct
     1201 atggtaagtc tttttgttat tgttaaagag gttaagcgga atgttaatat gttttagcta
     1261 aaaaatgtag tgaaaaatga gtacgtagac tattgtaata gtaattccgt aaaattgttg
     1321 aaaaagaaaa tgggaaatac cttgtcaaat taagcaccct atgatagaat agataggtat
     1381 gtggtgctag cacatccgta aacatattct acgtaggagg aaacagatca aaatggctaa
     1441 agtatgttat tttacaggac gtaaaaccgt ttctggaaac aaccgttcac acgcgatgaa
     1501 ccaaacgaaa cgtacagtta aaccaaacct tcaaaaagtt acgatccttg ttgatggtaa
     1561 acctaaaaaa gtttgggctt ctgctcgtgc gcttaaatct ggtaaagtag aacgcatcta
     1621 atagaaataa gccttagggc tttttttatt agcttatttc tatccataag ctagtcaggg
     1681 taataaacgc tatcgttaac tattctatct cttttacatt acttctaaaa tatgataaaa
     1741 taggatgata aaaacttttt gaggtagtaa ctatgactgt aaaaattaat acaaaagatg
     1801 gtctaatcga gctatccgac gatgtgattg caactgtcgt gggaggatca gcaacggaaa
     1861 tttttggtgt tgttggaatg gccagcaaaa gtgcaataaa agacaatttt caatcactac
     1921 ttcgcaaaga aaactacgct aagggtgttg tagtaaaatc aacagatctg ggtatttctg
     1981 ttgatgtcta cactgtgatg agttatggtg tgaaaatcag tgaagtctct aaaaatatcc
     2041 aagaacgcgt gaaatttaac cttgaaagtc agcttggtct cactgctgat atggtcaatg
     2101 tttacgtgca aaatattaag gttgtaggag aaaattagtg tcaaatatta caacaagctt
     2161 attccaagaa atggtccaag cggcagcgac tcgccttggc aaacaagcag aatatgttaa
     2221 ttccttaaat gtttttcctg ttccagatgg tgatacagga acaaacatga gtatgactat
     2281 ggacaatggt gccaaggaag ttgctgataa acctgcgtca actgtgggag aagttggaca
     2341 aatgttatca aaaggtcttt tgatgggagc gcgtggaaat tctggagtta ttacttcaca
     2401 actttttcgt ggttttggtc aaagtattaa gggaaaagat gagctgacag gtaaagattt
     2461 ggcacaagcc tttcaagtgg gtgtagaagt tgcctacaaa gcagtgatga aacctgttga
     2521 agggacaatc ttgacagtat ctcgtggagc agcaaccgct gccttaaaaa aagcggatct
     2581 tacagacgac gccgtggaag tcatgcaagc tgctttagac ggtgccaaag gtgcccttgc
     2641 taagacacct gatttattac cagtccttaa agaagttggt gtggttgatt caggcggtca
     2701 aggtcttgtc tttatctatg aaggtttcct atctgctttg aatggcgact atgtgacttc
     2761 agcagacttt aaagcgactc ctgctaacat gtctgaaatg attaatgctg agcatcacaa
     2821 atccgttgtt ggtcatgtgg ctactgaaga cattacctac ggttattgta ctgaaatcat
     2881 ggttgccctt aaacaaggcc caacctatgt gaaagaattc aactatgatg agtttcaagg
     2941 ctaccttagt ggccttggtg actcgctgtt agtggtcaat gatgatgaga ttgttaaagt
     3001 tcacgtccat actgaagacc ccggacttgt gatgcaagaa ggtcttaaat atggttcgct
     3061 catcaagatt aaagtggaca atatgcgtaa ccagcatgaa gcacaggttc aaaagactga
     3121 tgtcgaaaaa aacaaggctg aagtaaaaga atttggcttg attgcagtag tagctggaga
     3181 aggtttatct gaaattttca aggcgcaagg tgttgattac gtgatttctg gtggtcaaac
     3241 catgaatcca tctacagaag atattgttaa agctattgaa gcagttaatg ccaaacaagt
     3301 cattatcttg ccaaacaata aaaatatctt tatggctgcc caatctgctg cagaagtggt
     3361 ggatatcccg gctgctgttg ttgcaacacg tactgttccg caaggattta ccagcttgtt
     3421 ggcctttgat ccttcaaaat ctttagagga caatgtagca gatatgtcaa ctagtctttc
     3481 tgacgtggtc agtggaagcg ttactttggc agtccgtgac accaccattg atggtttaga
     3541 aatccatgaa aatgatttct tgggaatggt tgatggtaaa atcatcgttt cagacccaga
     3601 catggaagca actctaaaag ctgcctttga aaagatgatt gacgaagata gcgagattgt
     3661 gactatcttt gttggtgaag aaggtgatca agacttagca gaagaacttg ctgggtattt
     3721 aggagagact tacgaagatg tggaagttga gatccatcaa ggagatcagc ctgtttaccc
     3781 atatctcatg agtgtagaat aatagaatac aataaaaaaa gaggagttag gcaaaggtct
     3841 gactcttttt gtctatattg atgtcgtaat gagtggtttt agtgatggta agcccctaaa
     3901 aaatgagggt aatccatcac
//
LOCUS       DAUVUY010000120         3781 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.121, whole genome shotgun sequence.
ACCESSION   DAUVUY010000120 DAUVUY010000000
VERSION     DAUVUY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.121"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..359
                     /locus_tag="VOX58_001813"
     CDS             <1..359
                     /locus_tag="VOX58_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HES5879014.1"
                     /translation="APQRPDVLVGGQSEPIDITQDTQPGMSGSNDATVINEDTKPKRV
                     FHFDNKEPQASEKAAEQKLAPHDSHTTPQASDDTAAPHLPASGDKREASFTIVALTII
                     GAAGLLSKKRRNTDKN"
     gene            501..797
                     /locus_tag="VOX58_001814"
     CDS             501..797
                     /locus_tag="VOX58_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HES5879015.1"
                     /translation="MTKQDQLIVEKMEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADVLDLAPIMYKHM"
     gene            946..1230
                     /locus_tag="VOX58_001815"
     CDS             946..1230
                     /locus_tag="VOX58_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HES5879016.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            1664..2932
                     /locus_tag="VOX58_001816"
     CDS             1664..2932
                     /locus_tag="VOX58_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HES5879017.1"
                     /translation="MFQLRKKMTRKQLTLLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATTEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            2919..3593
                     /locus_tag="VOX58_001817"
     CDS             2919..3593
                     /locus_tag="VOX58_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HES5879018.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            3606..>3781
                     /locus_tag="VOX58_001818"
     CDS             3606..>3781
                     /locus_tag="VOX58_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5879019.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTI"
BASE COUNT         1387 a          736 c          768 g          890 t
ORIGIN      
        1 cagcacctca gcgcccagat gtccttgtag gtggtcaaag cgagccaatc gatatcaccc
       61 aagataccca accgggcatg tcaggctcta atgacgctac cgttatcaat gaagatacaa
      121 aaccaaaacg cgtcttccac tttgacaaca aagagccaca agcatcagaa aaagcggcag
      181 agcaaaaact tgctcctcat gatagccaca caacccctca agcttcagat gatacagcag
      241 cccctcactt gcctgcatct ggagacaaac gtgaagcatc ctttacaatt gttgctctaa
      301 caattattgg agctgcaggt ttgctcagca aaaaacgtcg caacaccgac aaaaactagt
      361 cgctgttagg cacaacgcat cactaaggcc agtgcatttg cactggcctt agttgggttt
      421 ttatcaaata gagggttttt catgacaatt gttagcactt cggtgtaaaa taaaagtaat
      481 aaccagcaaa ggagcaacct atgacaaagc aagatcagtt aatcgttgaa aaaatggaac
      541 aaacctatga ggcctttagc ccaaagctag caaatctcat agaagcccta gatgccttta
      601 aagagcacta tgaggaatat gccacactta gaaattttta cagtagtgac gaatggtttc
      661 ggcttgcaaa ccaaccttgg gatgatatcc cgtgcggtgt cctatctgaa gacctcctct
      721 ttgatatgat cggtgaccat aaccaactac tagctgatgt cttagacttg gcccctatca
      781 tgtacaagca tatgtaacca ttgttaacgt cacagatact acgatgacag gacaaaaaaa
      841 gcttgaaaat ctcaaaaaaa aacagataaa aaacagataa tcgttagaaa aacagtagat
      901 gcgtctgtta aactaaaggt atcaaaacaa aaggagcatt tcatcatgaa attcaaaaaa
      961 gtattagtca ttcctgcatt agcattagca gcaacatgct ttttaacagc ttgtggcaca
     1021 aaaaaagatt ctaaaaaaga agaagttaaa gaaatcaaaa tgtctgacat caaagacgac
     1081 gcagttagca aaaaaacaaa agtggttgat ggcgaagaag ttacagaata caccacaaaa
     1141 gatggaaacg tgattcagat tcctgcagga aacgaagaag gcatggaatc aaaagatgct
     1201 ggtggatcag gcgctcctgc taaaaactaa gggttacaac aaagagcata aggtgactcg
     1261 caaacgttac gcatgacatg aggcagtctg cctaagatgg taactcactt atcataatgc
     1321 ctaaatagcc aaacacacta actcgcaaat gtacaaaatc atatacccgt gcttatgtga
     1381 gcgaacggtt atatgatttt tgttttccaa aaacaagaaa agaacgctaa aatagccagt
     1441 ttgtcgtgtt tcaagctacc aaaaaggaag tgctaagaag tgctaaaatg aaaaaaagat
     1501 ccattatacc tgtcatctta ctgttggtca tcggattaat cgttggtgtt aagcgtcatc
     1561 atgactagta ggtcttgcta gtgaccgttc aaaagggata acgttggtat ggtataacca
     1621 taaaaatcgt ataaaaagaa aaaatagcta ttgaggagaa agtatgtttc agttaagaaa
     1681 aaaaatgacg cgcaaacaat taaccttgtt gagtgctgga gtgttgacct gtgtggttgg
     1741 tggtagctac ttgataatga accatcaaca acaagaaatt gtctctagtg tcaacaaagt
     1801 aaaagcctta accataaaag aagccatgga acaaggaaaa gatatcagct tgaccttagc
     1861 tggcgaagta acagctaaca acagcagcaa agtcaaaatc gactcaagta aaggagaagt
     1921 caaagatgtc tttgtcaaaa aaggcgatgt tgtcaaagta gggcaaccct tgtttagtta
     1981 tgaaacgtca caacggttaa cggctcaaag ttcagaattt gatgttcaaa ccaaagccaa
     2041 tcaactccaa gttgctaaaa ccaatgcagc attgaagtgg gaaacctaca atcgcaaggt
     2101 caatgaaatc aataccctaa aatctcgcta caacactgca ccagatgaga gcttactaga
     2161 gcaaattcgc agcgcagaag acagtgtatc ccaagcacta agcgatgcca aaacagcaga
     2221 tagcgatgtc aaaaccgctc aaatcgaact cgataaagct aatgctactg ccacaacgga
     2281 aaaaggtaaa ctagagtatg acactgttaa gtcagacact gcaggaacca ttgttagcct
     2341 aaatactgat ttgccaaatc aatcaaaatc caaaaaagaa aatgaaactt ttatggaaat
     2401 tatcgacaaa tcaaaaatgt tagtcaaagg taacatcagt gaatttgacc gtgacaagtt
     2461 aaaaatcggt caaaaagtcg aagtgattga ccgcaaagac aactctaaaa aatggactgg
     2521 aaaagtaacc caagttggca acctcaaagc agaggaaaaa ggccaaggtc aaggccaagg
     2581 tggcaatgac caacaagaca atccaaacca agcaaaattc ccttatgtta tcgaacttga
     2641 ccaatcagac aagcagccac tcattggttc acacacctat gttaatgtgc tcaacaatgt
     2701 tccagaagct ggcaagatcg tattgaaaga aacctttaca atggcagaaa atggaaaaac
     2761 ctatgtgtgg aaagttgata aaaacaaggt caaaaaacaa gaaatcaaga ctaagccctt
     2821 ctcaaaaggt tatgttgagg tgacaagtgg cttgactatg caagataaga ttgctcagcc
     2881 gcttcctggc atgaaagacg gtatggaggt aggaagtatt gttaaacctt aaagatattc
     2941 gaaaaagcta tcatcttggg actgaagaat ttgcgatttt aaaaggaatc gatttagaag
     3001 ttaacgaggg tgacttttta gccatcatgg gaccatcagg ttcgggaaag tcaacattga
     3061 tgaatatcat tgggtgttta gataagcctg gctctggctc atatgccatt gaaggcagag
     3121 acgtgtcatc cttatctgat aatgaacttg ctgatttgcg taatcaaaaa atcggctttg
     3181 tttttcaaaa ctttaacctg atgcccaagc taacagcttg tcaaaatgtc gaattgccct
     3241 tgacttatat gaatgttcct aaaaaagagc gtcgcaaacg agcgctagag atgttaaagc
     3301 tagtaggact agaagaacgc agtgaattta aaccgatgga gctatctggt gggcaaaaac
     3361 agcgtgtagc gattgcaaga gctttagtca ctaacccgag ttttatcctt ggtgatgagc
     3421 caacaggtgc actagacaca aaaaccagcg tccaaatcat ggacctattt aaacaattca
     3481 atgataacgg caaaacgatt attatcatca cacacgagcc tgaagtagct gccttatgca
     3541 aaaagacggt gatcctaaga gatggtaata tagaacattc cgatatagag taaaggggga
     3601 aaataatgga agatattatt gcagctttaa gctctatatt atcacacaag atgcggtcaa
     3661 ttttgacaat gctagggatt attatcggta ttggtgccat tatcgcaatc ttttcaatca
     3721 tcgaaggtaa taccgaaaat accaaaagac agttgattgg tggcagcaat aacactatta
     3781 a
//
LOCUS       DAUVUY010000121         3639 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.122, whole genome shotgun sequence.
ACCESSION   DAUVUY010000121 DAUVUY010000000
VERSION     DAUVUY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.122"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..83
                     /locus_tag="VOX58_001819"
     CDS             <1..83
                     /locus_tag="VOX58_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HES5879020.1"
                     /translation="SLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(191..1093)
                     /locus_tag="VOX58_001820"
     CDS             complement(191..1093)
                     /locus_tag="VOX58_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HES5879021.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDVNDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            1267..2760
                     /gene="lysS"
                     /locus_tag="VOX58_001821"
     CDS             1267..2760
                     /gene="lysS"
                     /locus_tag="VOX58_001821"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HES5879022.1"
                     /translation="MSNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            2896..3036
                     /locus_tag="VOX58_001822"
     CDS             2896..3036
                     /locus_tag="VOX58_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879023.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(3133..3345)
                     /locus_tag="VOX58_001823"
     CDS             complement(3133..3345)
                     /locus_tag="VOX58_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HES5879024.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
BASE COUNT         1073 a          757 c          662 g         1147 t
ORIGIN      
        1 ccagtcttac tattcttgaa actaaggatt atttaactta tgactgttta atttggaatg
       61 ataaatcctt tctagaggtg taaaaccact agacaataat aaaaaaagct aacacattga
      121 tttcttactg acaagacact cactttgcta gcttttttta ttattatcta gtgggctatt
      181 atggtttcat ctatctggca tattttaagc gcaaaacttt ttcttgctgc ttggttaacc
      241 gaagcaagat aaccaccttg tcaatgctca tgttactttc aagtaaacgc tctgctgcca
      301 tcatcaagcc attattgatt cccatctgta aaattggatt tttcttgtca ttgacatcaa
      361 aaataaattt attatcaggt aaatcaaaga gtactttaac ggcaccaaag agctgttcaa
      421 agttatcaat agccacatca taaacaggct tagtgcctgg ttttagctga cgacctctat
      481 gattaaacca catggaatgc caaccaccat taaaggcacc catgatatca ttgtcataag
      541 agtccccaac atatagtgtg gtttgtgggt tcatgtcaaa ttgttctgct gccaggttaa
      601 aaatttcttt ttctggtttt tgaaagcctg ttgcttggct cacaatgaca cgcttaggat
      661 caatataatc ataaagccct aatttacgaa cttttttcag ctgatgttct gtgggcccat
      721 tggtaataat ccccatagga acattctttg attttaaaaa gtcaagggtc atacgcatct
      781 catctaacat ggtaatgttc tcaagttcgt gctcatagac ttcttgaaaa tgtactcccg
      841 cagcttcgtc gatttctcga tagccaaact caagcagtgt ttccttgcaa cgccagaatc
      901 ggaaatattc tgtggtccac tcccctgcca ttactcttgg gaatccaaca tctgaatagt
      961 gacgaaagcg aatataagct tgattcatta ctgacatatc aaaatctggg aagcactttt
     1021 ccatggcgat tcgataaggc gcttgctggt cgtaaattgt gtcatccaca tcaaaaacaa
     1081 tagcagtaat catgagtctc ttttcttttc tagtttaata tcccaatcat tatactattt
     1141 tccattaggc ttttcaattt cattttcaaa aaaatgcccc gctgcagtcg cattttccaa
     1201 cgaaatatag gtgtattcta gcccctttta tgatacaatg gaaaggaaat cgtattggag
     1261 gaaaatatgt ctaatcaaca catcgaagaa ttaaatgacc aacagattgt ccgtcgtgaa
     1321 aaaatgacgg cgctggctga acaaggaatt gatcctttcg gtaaacgctt tgaccgcaca
     1381 gccaattctg ctgaattaaa agaaaagtat gctgataaaa caaaagaaga attacatgag
     1441 ttaaacgaaa cagctattgt tgctggccgc ttgatgacaa aacgtggcaa aggtaaggtt
     1501 ggttttgctc atcttcaaga ccgtgaaggc caaatccaac tttatgttcg caaggactct
     1561 gttggcgaag acaactatga aatcttcaaa aaagccgatt taggcgactt tatcggcgtt
     1621 gagggcgaag tcatgcgtac agacatgggc gagttgtcaa tcaaggcaac caagttgact
     1681 cacctttcta aatcacttcg tcctcttcct gaaaaattcc acggattaac tgatattgaa
     1741 accatttacc gtaagcgtca ccttgattta atttcaaatc gtgaaagctt tgaccgcttc
     1801 gtgacccgtt ccaaaatgat ttcagaaatc cgtcgttatt tagacggtct tgatttccta
     1861 gaagtagaaa cgcctgtgct tcataacgag gctggtggtg ctgctgctcg tcctttcgtt
     1921 actcaccata atgctcaaaa cattgatatg gttcttcgta tcgcaacaga acttcatttg
     1981 aaacgtttga ttgttggtgg tatggaacgt gtctatgaaa ttggtcgtat cttccgtaac
     2041 gagggaatgg atgctactca taatcctgag ttcacttcta ttgaggttta ccaagcctat
     2101 gcagactatc ttgatatcat gaacctgaca gaaggcatta tccaacatgc tgctaaagct
     2161 gttaaaggtg atggccctat tgattatcaa ggaactgaaa tccgtatcaa tgaacctttc
     2221 aaacgtgtcc acatggtaga tgctatcaag gaagtgacag gtgttgattt ctggcctgaa
     2281 atgactgtcg aagaagctat tgcacttgcc aaagaaaaac aggtcccact cgaaaaacac
     2341 ttcacaagtg tcggacatat catcaatgct ttcttcgaag aattcgttga agaaaccttg
     2401 gtccaaccaa cctttgtgtt tggtcatcct gttgaagtat cgcctttggc taagaagaat
     2461 ccagaagata cccgcttcac cgaccgcttc gaacttttca tcatgaccaa agaatacgcc
     2521 aatgctttca cagagttgaa tgatcccatt gaccaattat ctcgttttga agcacaagca
     2581 caagccaaag aacttggtga tgacgaagca actggcattg actacgactt cgttgaagct
     2641 cttgaatatg gtatgccgcc aaccggtgga ctcggaatcg ggattgatcg tctttgcatg
     2701 ttattaacaa acacaacaac aatcagagat gtgcttttat tcccaacgat gaaaccttaa
     2761 cttttttaag atttgtattc atatcttaaa accgataagt ggcaatataa cataacgttt
     2821 tattatctct aaccagtctt cattaccatg aagactggtt tattaacagt agcttataga
     2881 aagtgactta acaagatgac caaagaatat atctcctaca ttagtgctgc tatcttcttg
     2941 ctttatggcc ttttagctgc caaatggatt tttcttgggc taggcttaat tttggtactt
     3001 gtcggccttg ctgatcgcct caattcaaaa aaataatcct tctattcgcc cctgtccttt
     3061 taacctagcg atgtcatcac caggttaaaa ggacaagggc gaacgaggga ttatgtttcg
     3121 attaaggtaa cattacttac gaaaagcgtc taagatggtt ttaaattctt cattagtaag
     3181 agtaatgcct ttccccattt tcgtatggtc tggactccac gtgcggatat cccatttcgc
     3241 ttctgcgcca ttaaagctga cgcggttcaa ttctttcgtc caacctttat cattttctga
     3301 taaggtgagt aaatgttcct caatgttgaa tgtaaattct gccattagtt ttcctcctca
     3361 tctatactat tcgaaattaa agtggaaaaa atcatctttt tttcactttt tttatgactc
     3421 attctcctat tattctatca agttttgaga aaataagcta ttgtgatatc caattttcaa
     3481 aaaaaaacag gctctctttt cccaactatg gtcgtatacc atttggaaaa agagggcctc
     3541 tagtatttct tgttactatg ttctaacagt tttactggtg ccatcttttt gatagaggtt
     3601 gacaaggcct tccttgcaag ccctaatcat atcccctgg
//
LOCUS       DAUVUY010000122         3557 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.123, whole genome shotgun sequence.
ACCESSION   DAUVUY010000122 DAUVUY010000000
VERSION     DAUVUY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.123"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..357
                     /locus_tag="VOX58_001824"
     CDS             <1..357
                     /locus_tag="VOX58_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HES5879025.1"
                     /translation="ETEVVFNTQSLRHAMERAFLISNATQNGTVKLEITQNHISAHVN
                     SPEVGKVNEDLDIVSQSGSDLTISFNPTYLIESLKAIKSETVKIHFLSPVRPFTLTPG
                     DEEESFIQLITPVRTN"
     gene            432..629
                     /locus_tag="VOX58_001825"
     CDS             432..629
                     /locus_tag="VOX58_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HES5879026.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            959..2074
                     /gene="ychF"
                     /locus_tag="VOX58_001826"
     CDS             959..2074
                     /gene="ychF"
                     /locus_tag="VOX58_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HES5879027.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            2144..2713
                     /locus_tag="VOX58_001827"
     CDS             2144..2713
                     /locus_tag="VOX58_001827"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HES5879028.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            2716..>3557
                     /locus_tag="VOX58_001828"
     CDS             2716..>3557
                     /locus_tag="VOX58_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HES5879029.1"
                     /translation="MNILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLGNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GVFINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLF"
BASE COUNT         1225 a          523 c          696 g         1113 t
ORIGIN      
        1 gagacggagg ttgttttcaa tacccaatcc cttcgccacg ctatggaacg tgccttcttg
       61 atttctaatg ctactcaaaa tggtactgtt aagcttgaga ttactcaaaa tcatatttca
      121 gctcatgtta actcacctga ggttggtaag gtaaacgagg atttagatat tgttagtcag
      181 tctggtagtg atttaactat cagcttcaat ccaacttacc ttattgagtc tttaaaagct
      241 attaaaagtg aaacagtaaa aattcatttc ttatcaccag ttcgaccatt caccctaaca
      301 ccaggcgatg aggaagaaag ttttatccaa ttaattacac cagtacgaac aaactaagta
      361 agaaaaagct cccttttagg agtttttttg ttattataaa tattaacgat aatataagtg
      421 gagaaaaggg aatgtatcaa attggatcat ttgttgaaat gaaaaaacct catgcttgtg
      481 taattaaaga aactggtaaa aaggctaatc aatggaaggt actgagagta ggagctgata
      541 ttaaaattca gtgtactaac tgtcagcatg tgattatgat gagtcgttat gattttgagc
      601 gaaaattaaa aaaagtactg caaccatgaa agtcctttta aataaataat tctagctata
      661 tttgcaacca atactttcct aaaaaattgc tagtatgccg ttggaaaatt agctattcta
      721 atgttatcga aagaagaaag atggccattg acaatgacga aagttgtagc acagttactt
      781 agtttctcgg caaactatat caaaatgaga ataatacacc aaactttgat aatttaaatg
      841 aaggtgaaag agtggcataa tgataagcaa atctgaaatt ttcttcttat tcataccgct
      901 ttttcttcta ttttttggta taataatctt gattgaaatt tgaatggaga ttcgctaaat
      961 ggctttaaca gcaggtattg taggcttacc aaatgttggt aaatcaacct tatttaacgc
     1021 aattacaaaa gcaggagcag aagctgctaa ttatcctttt gcgactattg atcccaatgt
     1081 tggtatggta gaggttccag atgagcgtct gcaaaaattg acagaattga ttacacctaa
     1141 aaaaacagtt ccgacaacct ttgaatttac tgatattgca ggtatcgtta aaggtgcttc
     1201 tagaggggaa ggtctaggaa ataaattttt agctaatatt cgtgaagtcg atgctattgt
     1261 tcatgtggta cgtgcttttg atgatgaaaa tgtcatgcgt gaacaaggtc gtgaggatgc
     1321 tttcgttgat cctatagcag atattgacac tattaatctt gaattaatct tagctgattt
     1381 agagtcaatc aataaacgtt atgcgcgtgt tgaaaaaatg gcacgaactc aaaaagataa
     1441 agaatcagta gcagagttta atgttttgca aaagattaaa cctgttttgg aagatgggaa
     1501 atcagctagg acaattgagt ttacagaaga tgaagcaaaa gttgttaaag gtctcttttt
     1561 attaacaact aaacctgttt tatatgtagc taatgtcgat gaagataaag ttgctaatcc
     1621 agatggtatt gattatgtca aacaaattcg tgactttgca gctactgaaa atgctgaagt
     1681 agttgttatc tcagcgcgtg cagaagaaga aatttcagag cttgacgatg aggataaaga
     1741 agaatttttg gaagctatcg gtcttactga atcaggcgtt gataaattaa ccagagcagc
     1801 ttatcatctc ttaggccttg gaacctattt tacagcaggt gaaaaagagg ttcgtgcttg
     1861 gacgtttaag cgtggtataa aagctccaca agctgctggt attatccatt cagattttga
     1921 aagaggtttt attcgtgcag taaccatgtc ttatgatgat ctaatgacct acggttcaga
     1981 aaaagccgtc aaagaagctg gacgcttgcg tgaagaagga aaagaatatg ttgtccaaga
     2041 tggggacatc atggaattca gatttaatgt gtaattatat taaaacaata tcagaaggtt
     2101 ggaagaacat tccagccctt ttggcatttt agaaagagaa aatatggtaa aaatgattgt
     2161 tggtctggga aatccaggct ctaaatatga aaaaacaaag cacaatattg gttttatggc
     2221 tattgacaat attgtcaaga accttgacgt tacctttaca gatgataaaa attttaaagc
     2281 acagatagga agtactttta ttaatcatga aaaagtttac tttgtgaaac ctactacttt
     2341 tatgaataat agcggcatag cagtaaaagc attactaacc tactataata ttgacataac
     2401 agatttaatt gttatctatg atgatttaga catggaagtc agtaaattac gtttacgtag
     2461 taagggttca gcaggaggac ataatggcat taagtcaatc attgcccaca ttggaactca
     2521 ggaatttaac cgaatcaaag ttggtattgg acgaccttta aaaggtatga ctgttattaa
     2581 ccatgtgatg ggccaattca ataccgaaga taatattgct atttcgttaa ctcttgacag
     2641 agttgtcaat gctgtcaagt tttatttaca agaaaatgat tttgaaaaaa caatgcagaa
     2701 atttaatgga taatcatgaa tattttagaa ttatttagtc agaataagaa agtccaatcc
     2761 tggcactctg gattaaccac cttaggaaga caactggtaa tggggttatc gggttcaagt
     2821 aaagcattgg ctatagcttc cgcttattta gatgatcaaa aaaaaatagt tgtggttaca
     2881 tcaactcaaa atgaggttga aaaattagcc agcgatttat ctagtttact tgatgaagaa
     2941 cttgttttcc aattttttgc agacgatgtg gctgcagcgg aatttatctt tgcgtcaatg
     3001 gataaagctc tatcaagaat agaaaccctg caatttttag ggaatcctaa atctcagggc
     3061 gttttaattg ttagtttatc aggcttaaga actttattgc caaacccaga tgtttttaca
     3121 aagagtcaga ttcaactaac agttggagaa gattatgata gtgatactct tactaaacaa
     3181 ctgatgacaa ttggctatca gaaggtctca caggtcatta gtccgggaga atttagccgt
     3241 cgaggggata ttttagatat ctatgagatt acacaagaat tgccttatcg attggaattt
     3301 tttggcgatg atattgatag tattaggcaa tttcatccag aaactcaaaa atcttttgaa
     3361 caactagaag gtgtttttat taatccagca agtgatctta tttttgaggc tagtgatttt
     3421 caacgtggca ttgagcaatt agaaaaggct ctacaaacag cacaagatga taaaaaatct
     3481 tatttagaag atgtattagc tgtttcaaaa aacggtttta aacataagga tatccgtaaa
     3541 tttcaatcat tatttta
//
LOCUS       DAUVUY010000123         3445 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.124, whole genome shotgun sequence.
ACCESSION   DAUVUY010000123 DAUVUY010000000
VERSION     DAUVUY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.124"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(213..1844)
                     /gene="groL"
                     /locus_tag="VOX58_001829"
     CDS             complement(213..1844)
                     /gene="groL"
                     /locus_tag="VOX58_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HES5879030.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            complement(1880..2170)
                     /gene="groES"
                     /locus_tag="VOX58_001830"
     CDS             complement(1880..2170)
                     /gene="groES"
                     /locus_tag="VOX58_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HES5879031.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            complement(2348..>3445)
                     /locus_tag="VOX58_001831"
     CDS             complement(2348..>3445)
                     /locus_tag="VOX58_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HES5879032.1"
                     /translation="DQALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSG
                     IPLEKLTQADSKKYLNLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMF
                     LGPTGVGKTELAKALAEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGE
                     LTQKVRNKPYSVLLFDEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSN
                     LGATALRDDKTVGFGVKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQD
                     NMRDVVKIMVQPLITTLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIED
                     KLSELILSRELTSGHTLKIGLSYGKLTFHIA"
BASE COUNT          959 a          784 c          611 g         1091 t
ORIGIN      
        1 attggctcca tgatagcaca tgacaaagaa aaacaatata ttttccaata cattaaaagt
       61 ctgtaaccac aaagattggc attttatatc acaactaaaa aaacaaagat gggatatcat
      121 gagtcgaaat ccaatctttg ttcttttttc atagtaagaa gcagaccttt agtctcaact
      181 tctttttcat aatccttaat gctatagaga gcttagaagc cgcccatcat tcctggatcc
      241 atacctgctg gcattgctgg cgctggcgca gctggttcag gcttattagc aacaactgct
      301 tctgttgtca aaataagact agctacagaa gctgcatttt gaagcgctga tcgtgttact
      361 ttgacagggt caatgattcc tgttttaatc atatcaaccc actcacctgt tgcagcatta
      421 aatcctgttc ctgcagggct gtttttcaac ttgtcaataa ctacggagcc ttcgtaccca
      481 gcatttaaag caatttgacg tacaggctct tctagagcac gaagcacaat gttacgtcca
      541 gtagcgtcat cgccctcaag ctcaagagca gctacttttt cgatcaccgt aataagtgct
      601 gttccaccac cagcaacgat accttcttca acggctgcac gtgtggcatt tagagcatcc
      661 tcaatgcgaa gtttcatttc ttttaaagct gtctctgttg gagctcctac tttgataaca
      721 gctacaccac cagctaattt cgccaaacgt tcttgtagtt tttcacggtc aaagtcagaa
      781 gttgttgttt ctaattgcga tttaatcagt gcaatacggt tagcaatagc ttctgaactt
      841 cctgaacctt caacaattac tgtgctatct ttatcaactg taatcttagc agcctgtcca
      901 agggctgtca ttgtagcatc ttttaattca agtcctagat cctctgtaat cactgtacca
      961 cctgtcaaga tagcaatgtc ttcaagcata gctttacgac gatcaccaaa tcctggcgct
     1021 ttgacagcaa ccacattgaa agtaccacga atcttgttca agacaagggt tggaagggct
     1081 tcaccatcca catcatctgc aataatgagt aatggacggt tggttttaag aacttcctca
     1141 agtagtggca aaatgtcttg gatgtttgac acttttttat ccgtgattaa gataaatggg
     1201 ttttcaaggt ctgcaaccat tttttcattg tctgtgacca tgtattgaga caggtaacca
     1261 cggtcaaatt gcatgccttc gaccacttca agttctgttt ccatacctcg agattcttcg
     1321 atggtaatca caccatcgtt gcccacacgc tccatagctt ctgagatata ctctccaact
     1381 ttttcagagc gtgatgatac tgcagcgacc tgagcaatag cttccttgcc agatacaggt
     1441 tgagcaatgg ctttcaaggc ttcaacagct gttgctgttg ctgtttcaat gcctcgacgg
     1501 ataccaattg gattagcacc tgctgtcaca ttttttagtc cttcatgaac aatggcttgt
     1561 gtcaaaactg ttgcagtagt cgtcccatca ccagcaatat cattggtttt agaagccact
     1621 tcagacacca attttgctcc catgttttca aaatgatctt ctaattcgat ctctttagca
     1681 atggttaccc cgtcattagt aattaaggga gaaccaaaag ctttttcaag aacaacatta
     1741 cgccctttag gaccaagcgt tactttgacg gtatctgcta acatatcaac tccgcgcacc
     1801 atggcagcac gcgcatctgc tgaaaattta atatcttttg ccatattcaa tccttctttc
     1861 tatttagaga tttgtcgttt tattttgtaa caatagcaat gatgtcagat tcacggatga
     1921 tagaaacttt ctcatcatct acggttactt ctaaatcatg cccattttca actaaaacct
     1981 cttgacccac tgaaactgaa ggtagcacgc tatcacctgt aatagtgcga acacccgttt
     2041 cactgacagc gaggactgtc gcttttcgtg ttgactcttt atgagtgcct gcaaggacaa
     2101 aaccaccgac tgtctgctct ttttcatcat caaatcttac gaccacacgg tcacctaatg
     2161 gttttaacat gaaattttcc tccaataaaa gaatctttag taaagttagc actcatgttc
     2221 atcgagtgct aactttatat tctattttat cacttggtca aaaatagtca agaaaaaaga
     2281 agttattttt gtaagaataa cctctttttt tggtgacatc aaggatgatc caccttttat
     2341 atggaaatta agctatgtga aacgttaatt tgccatatga taatccaatt tttagcgtat
     2401 gcccacttgt caattctcga gaaaggataa gctctgatag cttatcttct atctcagttt
     2461 gcagcgttcg acgtaatggt cttgccccca tatgctcatc atagccgacc tcggacaaat
     2521 gtttcaaggc caaaggctga attttaaggg taataccttt ttctgccaat gtagtaatca
     2581 gaggctgtac catgatttta accacatcgc gcatgttatc ttgggtcaga ctatgaaaga
     2641 ccactttttc atcaatacga ttgataaatt ctgggcggta agtttttctt aattcttcta
     2701 aaatacgttt ctccatagct tgatggtctt ggtgaatgcc tttgacccca aaaccgaccg
     2761 ttttatcatc acgcagggct gttgcgccaa gattgctggt catgataata atagtatttg
     2821 aaaaatcgac cttacgccca cgactatcag tcaatatacc atcatctaat acttgaagga
     2881 gaacgttaaa aatatcagga tgtgcttttt ccacttcatc aaaaagcaag actgaataag
     2941 gtttatttct aactttctgt gtcagttcac ctccttcatc atagccgaca taaccaggag
     3001 gtgctccatt aagcctagac gctgcgaatt tttccatgta ctcagacata tcaaaacgaa
     3061 taagcgctgc ttcatcatca aagagaactt ctgcaagggc ctttgctagt tctgtcttac
     3121 ctactcctgt tgggccaaga aacataaatg atccaatagg acgttttcct gttcgaatac
     3181 ctgactgatt gcgacgaatg gctcttgaaa tagccgtaac agcagcatcc tgaccaatca
     3241 cacgcttatg cagttctttt tctaaattga ggtatttttt actatcagct tgcgtcagtt
     3301 tttcaagtgg aattcccgat aacttactca aggttgccaa aatatcatct tctgtcactg
     3361 gtgttggttt tctcatctga ccttttgctt ctttagctaa gaggcgagat acttttttca
     3421 tatcaccatt gataagagct tggtc
//
LOCUS       DAUVUY010000124         3380 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.125, whole genome shotgun sequence.
ACCESSION   DAUVUY010000124 DAUVUY010000000
VERSION     DAUVUY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.125"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..471
                     /locus_tag="VOX58_001832"
     CDS             <1..471
                     /locus_tag="VOX58_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HES5879033.1"
                     /translation="QLAHKGQDTYHFPRAMITEDHLEFSFSGLKSAFINLHHNAKQKG
                     NELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQGLRDRLAQEITH
                     IEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQFPDSFVIN"
     gene            complement(642..911)
                     /gene="rpsN"
                     /locus_tag="VOX58_001833"
     CDS             complement(642..911)
                     /gene="rpsN"
                     /locus_tag="VOX58_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HES5879034.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(1132..1890)
                     /locus_tag="VOX58_001834"
     CDS             complement(1132..1890)
                     /locus_tag="VOX58_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HES5879035.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSCQSDTH"
     gene            2130..2909
                     /gene="udp"
                     /locus_tag="VOX58_001835"
     CDS             2130..2909
                     /gene="udp"
                     /locus_tag="VOX58_001835"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HES5879036.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            2930..>3380
                     /locus_tag="VOX58_001836"
     CDS             2930..>3380
                     /locus_tag="VOX58_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HES5879037.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQT"
BASE COUNT         1025 a          542 c          710 g         1103 t
ORIGIN      
        1 cagttggctc ataaaggtca agatacttat catttccctc gtgctatgat aacagaagac
       61 cacttagaat tttctttttc aggactcaag tccgctttta tcaatcttca tcataatgct
      121 aagcaaaaag gcaatgaact gattctagaa gatttgtgtg catcttttca agcagctgtt
      181 ctagatattt tattggccaa aacaaaaaaa gctctgagca gataccctgc aaagatgctt
      241 gtagtggctg gtggtgtagc ggcaaatcaa ggattacgag atagattagc acaagaaatt
      301 acacatatag aagttgtgat tcctaaacta agactctgtg gagataacgc gggtatgatt
      361 gctttagcag cagctattga gtatgacaaa cagcattttg ctaatatgag cctaaacgcg
      421 aagcctagtt tagcttttga ccagtttcca gacagttttg taataaacta gtcacacttg
      481 ctagttagta agatttataa tggataagaa tctccctttg aaaaaacaac ccggtatctt
      541 ggcataccgg gttgtcatga tgataagtaa ttggcttcta tggttactaa taattcagat
      601 aattacctgc aaaataattg tctgcctaag gtgaagtaag cttaccagct agctttagtg
      661 acgccaggaa gctgtccttt gtgagctaag tcacggaaat tgatacggct aacaccaaac
      721 ttacgcatgt aagcatgagg acggccgtca attttgtcgc ggttcttcaa gcggtttgga
      781 tttgaatcac gaggcaattt gcgcagtgat tcataatcgc ccttcgcttt taagtcacga
      841 cgaaggtcag cgtactgttc aatcagttgg agctgctttt ggtatttagc aatttttgac
      901 ttttttgcca ttgctttctc cttcttaata tttaataacc tcagagtcgt tatcagcgct
      961 ctgaatccac ttaactggta tttaactggt taagtggata tataatcatt atacaataaa
     1021 taaaagcaaa agcaaataaa aaaaattaac tagttatctt ttttagccat aatatcaaaa
     1081 aactccacat ctaagtgtgg gagtcaggta ttaataactg tttaaaggtg tttaatgagt
     1141 gtcgctttgg caactttgag attgaacttt taagtcaaat aaatcagcag gaacgtagtg
     1201 tttggttgag accaagatgt ctttaccatt gtagagagtt tcttggatta aaataaaagc
     1261 actattttct gatagagtat acttagttgc tgtgtaattg ccagtcttgg tatagccgat
     1321 ttgattgcag gaatgagaag ctttaccaga ttcatagatt ttctcttcta agaaagtaag
     1381 aaggggctct tcttgattga gatttatagc atatttagaa ataacttcaa tagggataaa
     1441 agataaacta taagcaatag ctttattttg gctatgatac cagcgatcaa caataatcac
     1501 aacaggagtt tcttgtttta gggatgctgt aatggcaaca gtgggcactt caatccgata
     1561 ttccaattca acctttgtga tgtcagatga taggcttgca tatatggggt gttgaagata
     1621 ttcgaaacct tggtgatatt tagtttcagg agtcttgagt ataaaatttc cctttcctct
     1681 gatatttttt atgagatgat cctcttggag gagggctagg gatttacgca gtgtcatccg
     1741 actgacattg agctgtctag caagttcagg ttctgatggc agttgcatgc caggactata
     1801 tgtaccatct tgaataagtt gaaaaatagt attatatact tgcacatgtt taagtttttt
     1861 gagttttttg gttaagtcgt tagtagacat atcacttacc tcattaataa tctttagtgg
     1921 agtttaactt gtctaaaaac tattttagca ctaacagtat tccttgatta gaattgtacc
     1981 catctatagt gttagtatct cacactcagg tactttttgt caaaaaagag atgaaaaaca
     2041 ccttaaaaaa gcgtttacaa aatagagtgt gtgtgatact atacaagtgt atcaaaaatt
     2101 agacaagtaa aatacaggag gtttttaaca tgcaaaatta ttcaggtgaa gtcggattgc
     2161 aatatcacct acagattcgt ccaggtgatg taggtcgtta tgttatcatg ccaggtgatc
     2221 caaaacgttg tgcaaaaatt gcagaacatt ttgataatgc agttcttgta gcagacagcc
     2281 gtgaatatgt tacgtataca ggtacattaa acggtgaaaa agttagtgtt acttcaactg
     2341 gtattggtgg tccatcagcg tctattgcga tggaagaatt gaaactctgt ggcgctgata
     2401 cctttatccg tgttggaaca tgtggtggta ttgaccttga tgttaaaggc ggagacatcg
     2461 ttattgcaac tggagctatc cgtatggaag ggaccagtaa agaatatgct ccgattgaat
     2521 ttcctgctgt agctgacttg gaagtgacaa atgctctagt taatgcagct aaaaagcttg
     2581 gttatacaag tcacgcagga gtagttcaat gtaaagatgc attttacggg caacatgagc
     2641 cagaacgtat gccagttagt tatgaattat taaataaatg ggaagcatgg aaacgtctgg
     2701 gaacaaaagc gtctgaaatg gaatctgcag ctctttttgt agcagcaagt catctaggag
     2761 ttcgttgtgg atcagatttc cttgttgttg gtaatcaaga acgcaacgct ttgggtatgg
     2821 ataacccaat ggctcatgat acagaagcag ctattcaagt tgctgttgaa gcactccgca
     2881 cacttattga aaatgataaa tcacaataag cgaggttttg gaagtcatca tgcaatttat
     2941 ttatagtatt attggtattt tattggtatt aggaattgtg tatgcaattt ctttcaatcg
     3001 taagagtgtt tctctaagtt taattggaaa agctcttatc gttcaattca ttattgcgct
     3061 aatcttagta cgtatcccac taggccaaca aattgttagt gttgtttcaa ctggagttac
     3121 tagcgtaatc aactgtggtc aagctggttt aaattttgtg tttgggtcat tagcagatag
     3181 tggcgcaaaa actggtttta ttttcgctat tcaaacgctt ggtaatattg ttttcttatc
     3241 tgccctagtt agtctacttt attatgtagg aatccttgga tttgtagtaa aatggatagg
     3301 taagggcgtt ggtaaaatta tgaaatcctc agaggttgag agttttgttg ccgtagctaa
     3361 tatgtttctt ggtcaaacag
//
LOCUS       DAUVUY010000125         3379 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.126, whole genome shotgun sequence.
ACCESSION   DAUVUY010000125 DAUVUY010000000
VERSION     DAUVUY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.126"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(59..1369)
                     /locus_tag="VOX58_001837"
     CDS             complement(59..1369)
                     /locus_tag="VOX58_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HES5879038.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLIAT
                     LLSALGAGFFTTMAICCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISTQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            1593..2465
                     /locus_tag="VOX58_001838"
     CDS             1593..2465
                     /locus_tag="VOX58_001838"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HES5879039.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLASGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            complement(2765..>3379)
                     /locus_tag="VOX58_001839"
     CDS             complement(2765..>3379)
                     /locus_tag="VOX58_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HES5879040.1"
                     /translation="VQRLMKVMGLAARIRRKRKYSSYKGEVGKKADNLIKRHFEGSKP
                     YEKCYTDVTELALPEGKLYLLPVLDGYNCEIIDFTLSRSPNLKQVQTMLEKTFPADSY
                     SGTILHSDQGWQYQHQSYHDFLESKGILPSMSRKGNSPDNGMMDSFFGILKSEMFYGL
                     EKSYKTLDKLEEAITDYIFYYNNKRIKAKLKGLSPVQYRTKSFQ"
BASE COUNT         1020 a          600 c          781 g          978 t
ORIGIN      
        1 cacttaattt tgtgtctact ttagtatacc aagtccaacc aaatgttaga caataaatct
       61 aacttaagga agctagcagc atgagaatcc agatggtcaa gagtgccaaa cttaagctga
      121 tgggcacacc cagaagaatt tgttttttga aagcaagact gcgttcctct atatggggaa
      181 gggagagttg aatgagggaa ccagctgatg aaaaaggaga gatattagta gatagagcgc
      241 caataatagt ggctgttgtg agtaagcgaa tatcaatgtg aggactttga gtactgatgg
      301 tagcaatgat gggaaagaga gttggggcta caacggatag ggtggaacta aagagtgaca
      361 tcactccggc tatcacacaa aagaacagag gtaaccagaa atgaggaatg gttgttgtta
      421 tgaggtgccc tatcagtgta actaaacctg acttgaccgc tagagacatt agtaagctca
      481 tgccgcagag catgataatt atagcccagg gaaccttagc caaaatggct tcttgcttcc
      541 ctaatttgag ccttaaggcg aggcagacca tgagtattga gacaaagcca atatcaaatg
      601 tttgtcgata agtagctatc catgcgatgt ttgggaaaat gagatgcaac aagggaaaaa
      661 gccaaaccaa aaccatgctg ctgatcatga gcaaggtggt ttgtctttgg accttgctga
      721 ggactggtgg ttggtcaata gtcaaggatg agtttgttct tcccttactg tagcggatgt
      781 aacaagataa taaaagcaag acgatgagtg ggtagataat gctgacgata aagatatgat
      841 tgccaagtga aaaagcttgc tcttcccatc ccatttgctt aaacaggcct tgaaagacaa
      901 tgcctgagcc actggttatc aaattagccc ctcctgaagc tccccaattg acggcttgag
      961 ctccaatcaa agggtgtttg tccgcttttt gacagagggt aatcgctaga ggacagcaaa
     1021 tggccatagt agtgaaaaat ccagcaccta aagcagacaa aagggttgct atcaggtata
     1081 aaatcatgta gagggcgtta gggtgggtgc gtgtgcggta gagaatgtgt tgagccaaaa
     1141 catcaagagt gccgttagtt gttgcaacgt tataaaagag agagacgcta aaaatggtaa
     1201 aaaagagtga ggttggccaa aaatgaagaa gttctttggg gcttaatccc atgagagtgg
     1261 ttgcgatgag gtaagaaaaa gcaatagcca gtaggccaat attgattttg gtgcggtaac
     1321 caattccaat ggctagagca atggcgctaa ttattattaa atgaatcatt gaattgtcct
     1381 ttagttagaa tatgtaaata ggttggcaat tttggcgctc aaaagaccac ctgtttccta
     1441 ttggtacttg actattatac acaaaaaaat tacaattgtc ttgacacttg ttggaattta
     1501 aaacgaggta ttcaggtttg tgatttccta cggaagttaa tagcaattgc ctctagtgct
     1561 tacaagtgat attaaaaata gaggacttag tgatgtcaat catttcaact gatttaaccc
     1621 cttttcaaat agatgataca ttgaaagcag ccttgcgaga agatgttcat tccgaagatt
     1681 acagtaccaa tgccattttt gatcatcatg gccaagccaa ggtgtcgctt tttgccaagg
     1741 aagctggtgt tttagcgggg ctaaccgttt ttcaaagggt ttttacccta tttgatgccg
     1801 aggtgacctt ccagaatcct catcaattta aggatggaga tcgtttggct agtggcgatt
     1861 tggttttaga aatcataggc tcggtgagaa gcctcttaac atgtgaacgc gttgccttga
     1921 attttttaca acatttatca gggattgctt cgatgacagc tgcttatgta gaagccttag
     1981 gcgatgatcg cattaaggta tttgatactc gaaaaaccac tcctaattta cgtctttttg
     2041 agaaatatgc cgtgagagtt ggcggtggct ataatcatcg ctttaattta tcagatgcta
     2101 tcatgctaaa agacaatcac attgcggcag taggtagtgt tcaaaaggcg attgctcaag
     2161 cgcgtgccta tgcccctttt gtgaaaatgg tcgaggtgga agtggaaagt cttgctgctg
     2221 ccgaagaagc tgcggcggcg ggtgttgata ttatcatgtt ggataatatg tcattggaac
     2281 agattgaaca ggccattact ctaattgcag gacgttctcg gattgaatgt tctggaaata
     2341 ttgatatgac cactattagc cgttttcgtg gtttagcgat tgattacgtc tccagtggta
     2401 gtttaaccca tagtgctaag agtctcgatt tttccatgaa gggtttaacc taccttgatg
     2461 tctaagttgc aaaataaact aactttttaa aggatgtctt tcctctagaa cgagttttat
     2521 gtcagatagt ttaaacgcct cttcaaatac tttctctgtt ttcacctgat tcgtgtccgc
     2581 ttattatacc aattccaagc attcacctga aaaaacgata gggagaggtt agtgttttta
     2641 ttgtaaaagt tccacgccaa atccatgaag aaaattttgt tttcagaaat cacgattcat
     2701 cataataaaa tcaatcaaag tcgttaacta ggagtgtact gaccccaaaa agttggacaa
     2761 ataattattg aaaggattta gttctgtatt ggacaggact aagtcctttt agttttgctt
     2821 tgattcgttt gttgttgtag taaaaaatgt aatctgtaat agcttcttca agcttgtcaa
     2881 gggttttata agacttttcg agtccataaa acatttcaga tttgagaatg ccaaagaagg
     2941 aatccatcat gccgttatcc gggctattcc ctttgcggga catggatggc agaatacctt
     3001 tagactctaa aaaatcatgg taggactggt gttggtattg ccacccctgg tcactatgaa
     3061 gaatagttcc gctgtatgaa tccgctggaa aagtcttctc aagcatggtt tgaacctgtt
     3121 tcaagttagg agaccgagac agagtgaaat caataatctc acagttatag ccgtcaagaa
     3181 caggtaataa gtagagtttt ccttcaggta aggccaactc cgtcacatca gtgtaacatt
     3241 tttcgtaagg tttagagcct tcgaagtgac gtttaatcag attatcagcc ttcttaccaa
     3301 cctctccttt gtaagaagaa tacttgcgct tccgacgaat gcgggctgct aggcccatga
     3361 ctttcatgag acgttgaac
//
LOCUS       DAUVUY010000126         3357 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.127, whole genome shotgun sequence.
ACCESSION   DAUVUY010000126 DAUVUY010000000
VERSION     DAUVUY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.127"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..185
                     /locus_tag="VOX58_001840"
     CDS             <1..185
                     /locus_tag="VOX58_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HES5879041.1"
                     /translation="LVINSDGLTNMISNDEIVTILGSKVSLDEKNQEMIDLANLRGGL
                     DNITIALVHNESEDVE"
     gene            182..2080
                     /gene="pknB"
                     /locus_tag="VOX58_001841"
     CDS             182..2080
                     /gene="pknB"
                     /locus_tag="VOX58_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HES5879042.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIVFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTAQTP"
     gene            2203..2895
                     /locus_tag="VOX58_001842"
     CDS             2203..2895
                     /locus_tag="VOX58_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HES5879043.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            2892..>3357
                     /locus_tag="VOX58_001843"
     CDS             2892..>3357
                     /locus_tag="VOX58_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HES5879044.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLIL"
BASE COUNT         1082 a          589 c          643 g         1043 t
ORIGIN      
        1 atcttgtcat taacagtgat ggtctaacca atatgatttc aaatgatgaa attgtgacta
       61 tcttgggcag taaggttagt cttgatgaaa aaaaccaaga aatgattgac ttagctaacc
      121 taagaggagg cctagataat attacaatcg ctttggttca caacgaaagt gaggacgtcg
      181 aatgattcag attggcaaat tatttgctgg tcgttatcgc attctgaaat ctattggccg
      241 cggtggtatg gcggatgttt atttagcaaa tgatttgatc ttggataatg aagacgttgc
      301 aatcaaggtc ttgcgtacca attatcaaac agatcaggta gcagttgcgc gtttccaacg
      361 agaagcgcgg gccatggctg aattgaacca tcccaatatt gttgccatcc gggatatagg
      421 tgaagaagac ggacagcaat ttttagtaat ggaatatgtg gatggtgctg acctaaagag
      481 atacattcaa aatcatgctc cattatctaa taatgaagtg gttagaatta tggaagaagt
      541 cctttctgct atgactttag cccaccaaaa aggaattgta cacagagatt taaaacctca
      601 aaatatccta ctaactaagg agggtgttgt caaagtaact gatttcggca tcgcagtagc
      661 ctttgcagaa acaagcttga cacaaactaa ttcgatgtta ggcagtgttc attacttgtc
      721 tccagaacag gctcgcggct ccaaagcgac gattcaaagt gatatttatg cgatggggat
      781 tatgctcttt gagatgttga caggccatat cccttatgat ggcgatagtg ctgttacgat
      841 tgccttgcaa cattttcaaa agcctcttcc atctattatc gaggagaacc acaatgtgcc
      901 acaagctttg gagaatgttg ttattcgagc aacagccaag aaattaagtg atcgttacgg
      961 gtcaaccttt gaaatgagtc gtgacttaat gacggcgctt agttataatc gtagtcggga
     1021 gcgtaagatt gtctttgagg atgttgaaag taccaaaccc ctccccaaag tggcctcagg
     1081 tcccaccgct tctgtaaaat tgtctccccc taccccaaca gtgttaacac aggaaagtcg
     1141 attggatcaa actaatcaaa cagatgcttt acagcccccc accaaaaaga aaaaaagtgg
     1201 tcgtttttta ggtactttat tcaaaattct tttttctttc tttattgtag gtgtagcact
     1261 ctttacttat cttatactaa ctaaaccaac ttctgtgaaa gttcctaatg tagcaggcac
     1321 tagtcttaaa gttgccaaac aagaactgta tgatgttggg ctaaaagtgg gtaaaatcag
     1381 gcaaattgag agtgatacgg ttgctgaggg aaatgtagtt agaacagatc ctaaagcagg
     1441 aacagctaag aggcaaggct caagcattac gctttatgtg tcaattggaa acaaaggttt
     1501 tgacatggaa aactacaaag gactagatta tcaagaagct atgaatagtt tgatagaaac
     1561 ttatggtgtt ccaaaatcaa aaatcaaaat tgagcgcatt gtaactaatg aatatcctga
     1621 aaatacagtc atcagtcaat cgccaagtgc gggtgataaa tttaatccaa acggaaagtc
     1681 taaaattacg ctcagtgttg ctgttagtga tacgatcact atgcctatgg taacagaata
     1741 tagttatgca gatgcagtca ataccttaac agctttaggt atagatgcat ctagaataaa
     1801 agcttatgtg ccaagctcta gctcagcaac gggctttgtg ccaattcatt ctcctagttc
     1861 taaagctatt gtcagtggtc aatctcctta ctatggaacg tctttgagtc tgtctgataa
     1921 aggagagatt agtctttacc tttatccaga agaaacacac tcttctagta gctcatcgag
     1981 ttcaacgtca agttcaaaca gttcttcaac aaatgatagt actgcaccag gtagcaacac
     2041 tgaattaagc ccatcagaaa ctactgctca aacaccttaa caaagaatag ttagtctcat
     2101 gttttactag catgccacta actattcttt ttaactactc gtcatataaa gatgcaattt
     2161 tccaggaatt ctgataaaat acaaagaaat aaggggaaac taatgaaaaa atttcaattc
     2221 tttttactta tcgagtgtat actgctagct atgggtatca tgacaatctt ggataatgat
     2281 ttatcaagtt ttatcctaat tctagttctt atcttattag cattacgctt ttataaccaa
     2341 gatagccgca ataatttttt actaactgtc agtttattgt ttcttttttt aattttcatg
     2401 cttaatccct acattatcat ggctgttctt ttggggatag tttacatttt cattaaccat
     2461 ttttcacaag tcaaaaagaa gaatcgcttt gccttgattc gtttcaagga agaaaagatt
     2521 gaagtgaaca ataccaagca tcaatggatt ggtactgcta attatgaaag tgattattat
     2581 tgttttgatg acattaacat cattcgaatc tcaggaaatg acacggttga cttaaccaat
     2641 gttattgtga caggaatgga taatatcatt gtgatacgta aaatttttgg aaatacgact
     2701 atattggtac ctattgatgt cactgttact ttagatgtta gttccatata cggaagtgtt
     2761 gattttttta gatgtcagca atatgatttg cgcaacgaat ctattaagtt aaaagaaacg
     2821 gataaccaat cccttaaaaa agtcaaaatt atcgttacaa ctattgccgg aaatgttgag
     2881 gtggataggc gatgaaaaaa cgttactatg ctcttgtttg gctctactca accattacca
     2941 ttttatctat tgtttttgtg gtcatggata atttaggaat cacttttaac taccttcgca
     3001 atcatttatg gcaggtcgaa cgtctagggt tttcgatttt attgttgatt gtttctgtga
     3061 ctttattatt gttgttattg tggattatta tggatgataa cagtaagcgt aacatcaacc
     3121 aaaatctaaa atatattctc aataatcgac gactctatct agatgagaca tcagaaatca
     3181 atactaattt aagtcgactt tctaaaaaaa tgtctcacct gactgctaac atgcaaaaga
     3241 aagaaagtgc ttatattctt gatagccaag aagttgtaaa acaagagcgc aaacgaattg
     3301 caagagattt acatgacaca gtcagtcaag agctattcgc ttcgtcattg attttat
//
LOCUS       DAUVUY010000127         3163 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.128, whole genome shotgun sequence.
ACCESSION   DAUVUY010000127 DAUVUY010000000
VERSION     DAUVUY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.128"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..173
                     /locus_tag="VOX58_001844"
     CDS             <1..173
                     /locus_tag="VOX58_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HES5879045.1"
                     /translation="VAIADLSESLSDLNSQARHFGQKATNATGNVSKAGKLALVGKVA
                     SKVFGKKGEKHE"
     gene            166..594
                     /locus_tag="VOX58_001845"
     CDS             166..594
                     /locus_tag="VOX58_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HES5879046.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEEDIIIDYTEQDEPVSDTLDKH"
     gene            complement(754..1038)
                     /locus_tag="VOX58_001846"
     CDS             complement(754..1038)
                     /locus_tag="VOX58_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HES5879047.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLFAKVIKSLLM"
     gene            1237..2163
                     /locus_tag="VOX58_001847"
     CDS             1237..2163
                     /locus_tag="VOX58_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HES5879048.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            2265..>3163
                     /locus_tag="VOX58_001848"
     CDS             2265..>3163
                     /locus_tag="VOX58_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HES5879049.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIIICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQR"
BASE COUNT          995 a          545 c          701 g          922 t
ORIGIN      
        1 ttgttgctat tgctgatttg tcagaaagtc tttcagattt aaatagtcaa gcaaggcatt
       61 ttgggcaaaa agcaactaat gctacaggta atgtttcaaa agctggaaaa ttagcattag
      121 ttggtaaagt agcctcaaaa gtatttggaa aaaaaggaga aaagcatgaa taaaagtttc
      181 aaaaacttag tgattggagc tgtttcagga gtagcggcag cttacttctt atcaacagaa
      241 aaaggcaagg ctttgaaaaa tcgtgctgaa aaagcttatc aagcctacaa agaaagtcca
      301 gatgattacc accaatttgc taaagaaaaa ggtagtgaat acagccattt agcacgtgac
      361 actttctatg atgtgaaaga taagttggct tctggtgact taactaaaga ggacatgtta
      421 gacttgctca aagataaaac aacagctttc gtgcaaaaaa caaaagaaac ccttgccgaa
      481 gttgaagcga aagaaaagca agatgatgtt atcattgatt taaatgaaga agacatcatc
      541 attgactata ctgaacaaga tgagcctgtt agtgatacac tagataaaca ctaactaaaa
      601 cgacctaaaa catagaagaa gaaaagattg aaggatctga tcactagtcc cgatggttag
      661 ttttaggcta actgaagaag ggggggattg tggcttcaat ctttttttgt ttttgacaca
      721 aaaaaaagag tcacgtaaaa cggactctgg aagttacatc agtaatgatt tgataacctt
      781 ggcgaataag gaagtaatgc ctaagctgat gatgcttgca aataagaaag caaacattgg
      841 cgctaaattc attacgagca ggacaaatgc aaataaaacg gtaataaggc tgaaactagc
      901 gatacgagca aagaagacta attcttttag ctttgcactt tggccattga actcttgaaa
      961 atctcgaaat ttactttgag attcttggta agaatcatcc tgttcaaccg aattatgctt
     1021 ctttaacggt gcaggcatag tcctctcctt tccttaacat actaagtaat tctatcataa
     1081 aagtctcttc ttgtcatctt tttttaaaag atttgaaaag atttactaat gacaagtgac
     1141 ttatcatgct ataacagtaa ggggactagc ttaacgtttg ggcttttgca agaaatatgc
     1201 ttaaggtctt aaaatttttg gtataataaa gcttatatgg aaaaaataat tattacagcc
     1261 acagctgaaa gcattgagca agtcaaagct ttattagctg ctggtgttga tcgtatttat
     1321 gttggtgaag ccaattacgg gttacgcctt ccccataatt tttcttatga tgaattgaga
     1381 caaattgcca aactagtgca tgatgcaggc aaagagttga cagtagcttg caatgctttg
     1441 atgcatcaag atatgatgga tcagatcaaa ccctttttag acttaatgat agagattgct
     1501 gttgattatc ttgtggttgg tgatgcaggt gtgttttatg tcaataaaag ggatggctat
     1561 aactttaaac tgatttatga tacctctgtt tttgtgactt ctagccgtca ggtaaatttc
     1621 tgggggcaac atggtgctgt tgaaagtgtt ttagcaaggg aaatcccatc agcagagtta
     1681 ttcaccttag ctgaaaattt ggaatttcca gcagaggtgc tagtttacgg cgcctcggtg
     1741 attcatcact cgaaacgtcc cttgctagaa aattattacc attttacaaa gattgatgat
     1801 gaggttagtc gtgaacgagg acttttcttg gcagaaccag gagatgctag cagccattac
     1861 tccatttatg aagacaatca tggcactcat attttcatta ataacgatat cgacatgatg
     1921 tctaaattgg gtgagctgta tgcgcatggc ttaactcatt ggaagctaga tggcatttac
     1981 tgtccaggtg atgattttgt cgctattact aaattgttta ttcaggctaa aaccttatta
     2041 gaagctgggc aatttaccca agaagaagct gaaaagcttg atcaagcagt gcatgcccat
     2101 catccagctg gccgtggatt agatactggc ttttatgagt tcgatccaaa aaccgttaaa
     2161 taagtaatcc ttgtcttgag tgagtttact atttcagggc aagacagggt tatgacacca
     2221 ttacacaggt aatctaccta tcttgaaaat agaaagaata tcacatgtca catatgaaaa
     2281 aacgtcccga ggtcttatca cctgctggaa cacttgaaaa attaaaagtt gcgattgact
     2341 atggcgcaga tgctgttttt gttggaggac aggcctatgg tctaagaagc cgcgctggta
     2401 acttctctat ggaagaattg caagaaggca ttgattatgc acatgcgcgt ggagctaagg
     2461 tctatgttgc tgccaacatg gttacccacg aagggaacga aattggtgcg ggcgagtggt
     2521 ttcgtcaact gcgtgatatg gggcttgatg cggtcattgt ttcagatcca gccttgatta
     2581 ttatttgttc aacagaagcc ccaggtttgg aaattcattt gtcaacgcaa gcttcatcta
     2641 ccaattacga gacctttgaa ttttggaaag ccatgggttt gacccgagtt gttttagctc
     2701 gtgaggttaa tatggccgag ttagcagaaa tccgtaagcg gacagatgtg gaaattgaag
     2761 cctttgtcca tggagccatg tgtatttctt attcaggccg ctgtgttttg tcaaaccaca
     2821 tgagtcaccg tgatgccaac aggggcggct gctcacagtc ttgccgctgg aagtatgatt
     2881 tgtatgacat gccatttgga ggagagcgcc gctccttaaa aggggaaatt ccagaagact
     2941 attctatgtc ctctgttgac atgtgtatga ttgaccatat tcctgacctg attgaaaatg
     3001 gggttgatag cttaaaaatt gaaggccgaa tgaaatctat ccactacgtc tcaaccgtaa
     3061 ccaactgtta caaggcggct gtagatgctt acatggaaag cccagaagct ttttatgcta
     3121 tcaaagagga attgattgac gagttgtgga aggttgccca gcg
//
LOCUS       DAUVUY010000128         3147 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.129, whole genome shotgun sequence.
ACCESSION   DAUVUY010000128 DAUVUY010000000
VERSION     DAUVUY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.129"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..879
                     /gene="galU"
                     /locus_tag="VOX58_001849"
     CDS             <1..879
                     /gene="galU"
                     /locus_tag="VOX58_001849"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HES5879050.1"
                     /translation="IIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEALKSGIE
                     EILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIRQSHPRGL
                     GDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATIAVMRVPH
                     EDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIFSILEKQE
                     PGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKHPQVKDDL
                     KAYIIQLGKALEKTKP"
     gene            complement(978..1649)
                     /locus_tag="VOX58_001850"
     CDS             complement(978..1649)
                     /locus_tag="VOX58_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HES5879051.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLSPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGVLLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
     gene            2337..>3147
                     /locus_tag="VOX58_001851"
     CDS             2337..>3147
                     /locus_tag="VOX58_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5879052.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFL"
BASE COUNT         1048 a          626 c          502 g          971 t
ORIGIN      
        1 attattcccg cagcaggttt aggcacacgt tttttacctg caaccaaggc ccttgccaag
       61 gagatgctac ctattgttga taaaccaact attcaattta tcgttgaaga agctctaaaa
      121 tcaggtatcg aagaaattct tatcgttact ggaaaatcca aacgctccat agaagatcat
      181 tttgattcta attttgaatt agaatataat ttacaggcta aaggaaaaat cgaacttcta
      241 aagttagtag acgagaccac ttctattaac cttcacttta ttcgacagag tcatccaaga
      301 ggattaggag acgcagttct tcaagcaaag acctttgttg gcaatgaacc ttttgttgtc
      361 atgctaggtg atgatctgat ggatattaca aatccaaatg taaaaccatt aaccaaacaa
      421 ttgatcgatg actatgaaga aactcatgct gcaactattg ccgtcatgag ggttcctcac
      481 gaggatgtct ctaactacgg tatcattgct cctcaagcaa aagcagtaaa aggactctat
      541 agtgtggata cttttgttga aaaaccacaa cctcaagatg cacctagtga tttggcaatt
      601 attggacgtt acttgctcac accagaaatt tttagtattt tagaaaaaca agaacctggt
      661 gcaggcaatg aagttcaact gactgatgct attgataccc tcaataagac acagcgtgtc
      721 tttgctcgtg agtttaaagg gaaacgctat gatgttggcg ataaatttgg gtttatgaaa
      781 acttctttgg attatgccct gaaacaccct caagtcaaag atgacttgaa agcctacatc
      841 atccaactgg gcaaagcact agagaaaaca aaaccataaa aataaggagg gaaaatccct
      901 ccttattttt atggtttttg agtcgtttca tcctactagc catctgagaa gtaacagtgc
      961 taaccactca cttattttta taaaaaccca agcactaaca caccaaccaa aataatacca
     1021 tagctcacta aagccaacat tcgcttggtt ttcttcactg ttatcactct catcttagtt
     1081 ggaaatacta tactcagtaa cacaccaccc acgacaccgc caatatggcc tgccatactc
     1141 acgtttggca taaaaagatt catcagtaaa ttgactacaa ttaacgtctg gtatgacttg
     1201 cccaaatctt tcaacgcttg gttcttaccc aaaaaactca atactacaat agcagcaaaa
     1261 agtccaaata acgacgtcga tgctcctgca gctactgttt caggactcaa ccaaaaagta
     1321 aaggcattgc ccataacacc tgataaaaca taaagtaata aaaaacggcg tgaaccccag
     1381 aggtcttcga caatttgacc aacaaaatat agtgttaacc cattgacaaa aaaatgacca
     1441 aagccgatat gaataaatat cggagtcacc aatcgccaca actgatctgg cattgctttt
     1501 actagtaatc caaacatacc tccaacttga taaatagctt gagcaccagt tgctaaatgt
     1561 ccatatacca cttgcattgc tataaaaatt aagcctgtta accctaaaag gaaaatagta
     1621 ataggatatc gttttaataa ccgtgtcatc taagataaac ctcctaaaca gcaatatcat
     1681 aaatcgtttg atttggcaat cgtattggcc cgctgataag cttgattgct aacaacatcc
     1741 actaataaag cttgatcatt ttgacgtttt aagtctacat ccatagttcg taaagactag
     1801 aagagttctt tgagaatctc ttttttcatc atcttcttat cataccataa attaggaaaa
     1861 aggttcaaga ctgacaagaa attagctctc tcaaaaaaga ttaaaaataa aggcattcta
     1921 aagaatcatt taagaaaact ttaaggttac tatcgtagaa tgaaaacatc ctaaaaagcg
     1981 gtttacctaa accgttggga gaataggttt tccccttgtt ttcccttttc ataaatctcc
     2041 tgggcaccta tcctcgtagg tgccctccct tgtttaaacc tgaccagtta accggctggt
     2101 taggttttta aatttaaaaa gtcaacagca aaaatatgat atcacccgcg tgttcttagc
     2161 tctctcaatt atgggctaaa aacacgcatc aatcaatcta ttgatgaagt ttgctgcttt
     2221 ttgatgatct ttattgtgac tatactggta atcctattga ttttagttga cttttacggt
     2281 agattcgcta caatatatag gattatacaa tttaatttaa ggagagaaaa attacattgt
     2341 tagattatta tttggaatcc gatattattg accagaaaat cctactggct attttgttga
     2401 cagaaaaaga actgacgatg gcagcagctt gttctcaaac aactttgact gctttaaaaa
     2461 taaagcagta tcttcgacaa tttaacgcct tatttaaagg gtacttacgt attgagctta
     2521 ttaaatctcg tatttattgt gaggtattaa atgataagcg agaagcattt ttttatgata
     2581 tttttgcctt atcagatact ctaaaaatgt tgacattctt attactagat aatcccaaac
     2641 acaaatcaat cgctgtttat acacgaaaac aagggatatc ccagtcaaaa gcttatcgtc
     2701 ttattcataa attaaaacat tatttacaag acattggttt aaatattgta gacaatactg
     2761 ttatcggaga tgaacttaaa atacggtatt tgattgctct tttacataaa gagtacggca
     2821 ttatattata tgatatacaa cctgcagaca ttgaaacgat tcatgctttt atctttgcag
     2881 cacaaaaaaa cttacaacct tctgcctttt tagatagacg cttcttgttt tttgatgtgc
     2941 tgttgatgtt gacttggaag cgccatcgct atcctgttca tttgcctcat ttagctcttt
     3001 ttgaacatct caaatccttg cctatttttg acaatatcaa aaccattgcc gtggatgagc
     3061 tagcacctcg aactcgtgtc acattttcgt ctgatgattt tgactatctc tttttaattt
     3121 atttaacgac tgataactcc ttcttga
//
LOCUS       DAUVUY010000129         3070 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.130, whole genome shotgun sequence.
ACCESSION   DAUVUY010000129 DAUVUY010000000
VERSION     DAUVUY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.130"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..1694)
                     /locus_tag="VOX58_001852"
     CDS             complement(<1..1694)
                     /locus_tag="VOX58_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HES5879053.1"
                     /translation="MKTKKVIILVGLLLSSQLTLISCQSRGNGTYPIKTKQSRKGMTS
                     NKIKPIKKSKKTNKTHKGMAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQQAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSPVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAYVADQYDPAKKAEKPAETPHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPEPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTSFPSNEKDPNKM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKRLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADV"
     gene            1898..2839
                     /locus_tag="VOX58_001853"
     CDS             1898..2839
                     /locus_tag="VOX58_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HES5879054.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFTENSQEK
                     RAIAIDLTDQGHLIVQFENGDFQTLRSGEISLSSWEN"
     gene            complement(2814..3017)
                     /locus_tag="VOX58_001854"
     CDS             complement(2814..3017)
                     /locus_tag="VOX58_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HES5879055.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
BASE COUNT          930 a          615 c          557 g          968 t
ORIGIN      
        1 acatcagcat ttcttgataa atcaacaacc gtcaatgatt ctttatgaga aacagggctt
       61 aaatctgtaa tctgattatt atcaatgtgc aattcttgca attgatgtaa cgatgccagt
      121 gggcttaaat cagaaatctt attattactc aatacgagga atttgagatt tggtaattga
      181 ccaagcggcc taatatcttc aataccatta tcagcagccg ctactagagt taagttttta
      241 tatttgctca agaaactaat atctttgaga ttgttttgtg aaatatcaat gccttctaac
      301 tgtggcatat tatccaaaaa tctataatca gtcacccctg tttttgtcat tagtaaccgt
      361 ttcaattttt taaattgcaa aacaggtgag atatctttga ttggtgtaaa gccaattcct
      421 aaagtttcta agttgggtaa cagtgaaagt cctttacgtt gcaaaggatc tttacggctg
      481 cccaagtcaa gtttgataac cgttgctaac cattctttca tcttattcgg atctttttca
      541 tttgatggga atgaggttgg agcatcgtga gtgcgaacga tatccaaaat cacttcttca
      601 tcaaacccta aggcacgcaa ggtatccatt ccaaccttat gtttttccaa ttcacgggca
      661 tgctcaatag catgtggttc tggaatgtct tttccgattt caatatcaga taacatcaat
      721 acgtgtgcgt ggtcatggtg agggtattcc aaaccaagtt taccgtcttg tgtttccaca
      781 cgtttaatag ttgatggatc aatccccaat ttttctgcca aataagctaa tttttcttgg
      841 tattcctttt cacgttcaga tagctctggt gtgtgagggg tttctgctgg cttttctgct
      901 tttttagcgg gatcgtattg atctgccaca tatgcccagc caccctgacg aaggtccgca
      961 aaagaaatag gatgtaagtg accatcgtgg tccactaaaa tactgtcttt tgttacccca
     1021 acaatacctt gaccgttaaa ttgaaaacca tctgaggttg ggaaatgcaa gcctggaata
     1081 ccattagctg tagctgttga aacagggcta ctggtttgtg gcaagcgagt agcaacctgt
     1141 ttagcttgtg cctgtgtctg acctgataag cttgacttca aaatataatg gaagtgatca
     1201 tcgtggcgaa ccgtgtagcc taaagcatct tcagccacaa tatccgctgg gttaaattca
     1261 taatggtgat gaggatctgc tactttagaa gtcccttgac tagctgcttg ctgagcaaca
     1321 gctggcttag ctaaacttgc tccttttgga ataaggtatt caaatggact tccctttaaa
     1381 tcggcataaa aaataaaatg cgaatgacca tcatggtcaa caacgattcc ctgatctgtt
     1441 tttgataaga tttttgagtc tttggttaaa ataaacccat catctgtagg aaaatcgaca
     1501 cccgccatac ctttgtgagt cttgtttgtc tttttgcttt ttttaatcgg tttaattttg
     1561 tttgacgtca ttcccttacg tgattgtttc gttttaatgg gatatgtacc attacctcgt
     1621 gattgacaag atatcaaagt caactgagat gataacaata gaccaactaa aataataact
     1681 tttttcgttt tcattcaacc ctccttttta tttaactggt taagtatatc acatcacttg
     1741 tttttcctca agctcttttt aggataacta ttagttgttg acgagttgga gctctgaagc
     1801 ttcaacaaac aatagtttct gttgttagta gcattacaca aaaaaactaa cttttctttt
     1861 cctttgaaaa taagatcttt tataatatac tatacatatg aaaacatcag aaaaaattta
     1921 tcaattactg tcacaaaccg atgacttcgt cagtggggaa tatctagcag atcaactcag
     1981 tatctctcgt acatccgttt ggaaatccat caaaagtctc gaaaatcagg ggattcaaat
     2041 tgattcttta aaacacaagg gctaccgcat ggtacaaggt gatattttat tacccaaaac
     2101 cattagccaa gggcttggga tgccagtcac ctatacccct cacagccaat ctacccaatt
     2161 agatgccaaa caaggaatcg aagctcataa cagcgctcca aggctttacc tagctcctag
     2221 ccaagaagct gccaaaggac gcctcgatcg gcaatttttt tcagctagca ccggtggtat
     2281 ttacatgtcc atgtacctaa aacccaatgt cccttatgct gatatgcctc cttatacgat
     2341 gatggttgct tccagtattg tcaaagctat ctcgcgatta acaggcattg atacagaaat
     2401 caaatgggta aacgacatct atctaggtaa ccataaagtt gcagggattc tgacagaagc
     2461 cattacttct gttgaaactg gtttaatcac cgatgtcatt attggggttg gtctcaactt
     2521 ttttgtcacc gacttcccag aagcgattgc tcaaaaggca ggttctctct ttaccgaaaa
     2581 accgactatc acgcgcaatg accttatcat tgacatctgg aaattattct tatctattcc
     2641 tgtcaaggac catgtcaaag tttacaaaga aaaatcactt gttcttaata aacaagtgac
     2701 ctttacagaa aatagtcaag aaaaacgtgc tattgcgatt gatctcacag atcaaggaca
     2761 cctcattgta cagtttgaaa atggtgactt ccaaaccttg cgaagcggtg aaatcagtct
     2821 ttcttcttgg gagaattagc cgtttttaaa atggttttag tcaattggtt aatggtatgc
     2881 actcttctta aatccctaaa gagcaacaag ccccaaaata aggcgaagag ataatttcca
     2941 gagagcaaca ggtcattaaa aaaataggtt tcaaaagcac atacaaaggc cataaaaaga
     3001 aattgtcgaa tagtcatagc tttattataa cagaaaaaaa tgattctatg aagaatccac
     3061 acagattgat
//
LOCUS       DAUVUY010000130         2982 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.131, whole genome shotgun sequence.
ACCESSION   DAUVUY010000130 DAUVUY010000000
VERSION     DAUVUY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.131"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1157
                     /locus_tag="VOX58_001855"
     CDS             <1..1157
                     /locus_tag="VOX58_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HES5879056.1"
                     /translation="AIITFNNLYPHSPSKINSGKATTSNMVFNNTTNTTKAVKAVQNA
                     VVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAELSIFSEGSGVIYRKDGNSAYVVT
                     NNHVIDGAKRIEILMADGSKVVGELVGADTYSDLAVVKISSDKIKTVAEFADSTKLNV
                     GEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKNENGETVSTNAIQTDAAINPGNSG
                     GPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGIGFAIPSTDVIKIIKQLETNGEVI
                     RPALGISMVNLNDLSTNALSQINIPTSVTGGIVVAEVKEGMPASGKLAQYDVITEIDG
                     KTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKADIKLTKTTQDLTKTQ"
     gene            1216..2022
                     /locus_tag="VOX58_001856"
     CDS             1216..2022
                     /locus_tag="VOX58_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HES5879057.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLL"
     gene            2255..>2982
                     /locus_tag="VOX58_001857"
     CDS             2255..>2982
                     /locus_tag="VOX58_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HES5879058.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNN"
BASE COUNT         1046 a          517 c          503 g          916 t
ORIGIN      
        1 tagcaattat tacattcaat aatctctacc cacattctcc ttcaaaaata aattcaggta
       61 aggcaacaac tagtaatatg gtttttaaca acacaaccaa tactactaaa gctgtcaaag
      121 ctgttcaaaa tgcagttgta tcagttatta attatcaaga taacccttct tcatcccttt
      181 ctaaccctta tacaaaactc tttggagaag ggcgttcaaa agagaataag gatgctgaat
      241 tatctatttt tagtgaagga tctggggtca tttatcgaaa agatggcaac tccgcttacg
      301 ttgttactaa taaccatgtt atcgacggag ctaaacgaat tgaaattctt atggcagacg
      361 gatctaaagt tgttggtgaa ttagttggag ctgatactta ttcggattta gctgttgtta
      421 agatctcttc agataagata aaaacagtag ctgaatttgc agactctaca aaactaaatg
      481 ttggagaagt tgctattgct atcggcagcc cactaggaac acaatacgct aattctgtta
      541 ctcaaggaat cgtctctagt ttaagtcgta ctgtaacttt aaaaaacgag aatggtgaga
      601 ctgtctcaac aaatgctatt cagacagatg ctgctattaa ccctggaaac tctggtggac
      661 cactaattaa tattgagggg caagtaatcg gaattaattc aagtaaaatt tcttctaccc
      721 caactggtag caacggtaat agtggtgctg ttgaaggaat tggttttgct atcccatcta
      781 ctgacgttat taaaattatt aaacaactag aaactaatgg tgaagttatc agacctgctc
      841 ttggaatatc catggtcaat ttaaatgact tatccacaaa tgctcttagc caaattaata
      901 ttccaactag tgtaactggt ggtattgtag tagcagaagt taaggaagga atgccggcat
      961 ctggcaaact tgctcagtac gatgtgatta cagaaattga tggcaaaaca gttaattcaa
     1021 ttagtgattt acaaagtagt ctatacggac acgatattaa tgatactatt aaagtaactt
     1081 tttatagagg aactacaaag aaaaaagcag atatcaaatt aacaaaaact actcaagatt
     1141 tgactaaaac gcagtaaaca agctacctaa atactattac attttatcac ataaaacagt
     1201 ccaaaggact gttttatgtt tcttattcta tccaggaact cccttatgac aaaagaatta
     1261 ttgatagacc taccaattga agatattgtt acaaatccct atcaaccaag gatacaattt
     1321 aatcaaagag aactccaaga cctagcaact tctattaaat caaacggcct cattcaacct
     1381 attattgttc gaaaatctga catttttggc tatgagttag ttgctggaga acgacgactt
     1441 aaagcttcaa aaatggctgg tctaaaaaaa gttccagcta tcatcaagaa gatctctaca
     1501 ctcgagagta tgcaacaagc tatagttgaa aatttacaac gttctaacct taacgctatc
     1561 gaagaagcta aagcctatca gttattggtt gaaaaaaaac acatgactca cgatgagatt
     1621 gctaaatata tgggaaaatc aagaccttat attagcaata ccttacgtct gttacaactc
     1681 ccagcaccta tcattaaagc aattgaagaa ggaaaaatta gtgcgggaca cgcgcgtgct
     1741 cttttaactt tgagtgatga taagcaacaa ctgtacctca ctcataaaat acaaaatgaa
     1801 ggcctaagtg ttaggcaaat tgagcaactg gttacttcta ctccaagttc gaagctatct
     1861 aaaaaaacta aaaatatttt tgccacttct ttagagaaac aattggctaa atcattggga
     1921 ctctctgtca atatgaagct gacagcaaac catagtgggt accttcagat atctttttcc
     1981 aatgatgatg aattaaacag aattatcaac aagctacttt agcttgttga tattctgttt
     2041 tttctttttt agttttccac atgaaaaata gttgaaaaca atagcggtgt cacattaaaa
     2101 tggcttttcc acaggttgtg gagaacccaa attaacagtg ttaatttatt ttccacaggt
     2161 tgtggaaaaa ctaactatta tccatcgttc tgtggaaaac tagaatagtt tatggtagaa
     2221 tagtgctaga attatccaca agaaggaacc tagtatgact gaaaatgaac aaattttttg
     2281 gaacagggtc ttggaattag ctcagagtca attaaaacag gcaacttatg aattttttgt
     2341 tcatgatgcc cgtctattaa aggtcgataa gcatattgca actatttact tagatcaaat
     2401 gaaagaactc ttttgggaaa aaaatcttaa agatgttatt cttactgctg gttttgaagt
     2461 ttataacgct caaatttctg ttgactatgt tttcgaagaa gacctaatga ttgagcaaaa
     2521 tcagaccaaa atcaatcaaa aacctaagca gcaagcctta aattctttgc ctactgttac
     2581 ttcagattta aactcgaaat atagttttga aaactttatt caaggagatg aaaatcgttg
     2641 ggctgttgct gcttcaatag cagtagctaa tactcctgga actacctata atcctttgtt
     2701 tatttggggt ggccctgggc ttgggaaaac ccatttatta aatgctattg gtaattctgt
     2761 actattagaa aatccaaatg ctcgaattaa atatatcaca gctgaaaact ttattaatga
     2821 gtttgttatc catattcgcc ttgataccat ggatgaattg aaagaaaaat ttcgtaattt
     2881 agatttactc cttattgatg atatccaatc tttagctaaa aaaacgctct ctggaacaca
     2941 agaagagttc tttaatactt ttaatgcact tcataataat aa
//
LOCUS       DAUVUY010000131         2963 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.132, whole genome shotgun sequence.
ACCESSION   DAUVUY010000131 DAUVUY010000000
VERSION     DAUVUY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.132"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            233..916
                     /gene="rpiA"
                     /locus_tag="VOX58_001858"
     CDS             233..916
                     /gene="rpiA"
                     /locus_tag="VOX58_001858"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HES5879059.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEVLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEVPKAS"
     gene            993..2204
                     /locus_tag="VOX58_001859"
     CDS             993..2204
                     /locus_tag="VOX58_001859"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HES5879060.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTVPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGSRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIIANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDIA
                     MIGESFLGHLK"
     gene            2220..2660
                     /gene="arsC"
                     /locus_tag="VOX58_001860"
     CDS             2220..2660
                     /gene="arsC"
                     /locus_tag="VOX58_001860"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HES5879061.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLTDPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYIKEDGEIVNPIEVKGEKDVIDD"
     gene            2644..>2963
                     /locus_tag="VOX58_001861"
     CDS             2644..>2963
                     /locus_tag="VOX58_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HES5879062.1"
                     /translation="MSLMTKINETKDFLVAKGIEAPEFGLILGSGLGELAEEVENPVV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEG"
BASE COUNT          872 a          549 c          656 g          886 t
ORIGIN      
        1 caaaaggtca gtaaaggtca aacaaaaaca cttgattttc aaaaagctaa ataaacacga
       61 aaagtaagtg cttatatgat gactttagaa taaatcataa gttttaaaaa gatcatccta
      121 ttctatagca cttaggctgt caatgagaga aaaacaaatg ttttcattaa caagttggcg
      181 ctttcatggt ataataaagt gctaagacgc aattctatta taagggggaa tcatggaagc
      241 actcaaaaaa attgccggtg ttaccgctgc gcaatatgtg actgatggca tgaccattgg
      301 gcttggaact gggtcaacgg cttactattt tgttgaggaa attggccgtc gcgtaaagca
      361 agaaggactg caagtggttg gtgtgactac gtcaagtgtg actagcaagc aagcagaagt
      421 cttaggcatt cctttaaaat cgatagatga tattgatagc attgacctca ccgtagatgg
      481 tgctgatgaa gtggataagg atttcaatgg aatcaagggt ggcggcgcag ccttactaat
      541 ggaaaaaatt gttgcaacac caacaaaaga atacatctgg gttgttgatg cttctaaaat
      601 ggtagaacat ctgggtgcct ttaagttacc agtcgaagtc gttcaatatg gtgctgatcg
      661 tttgttccgt gtctttgaaa aagctggtta caaaccgtct tttcgtatga aaggtgatag
      721 tcgtttggtg acagacatgc aaaactatat tattgatctt gatttgggtt gtattaaaga
      781 tcctgttgct tttggtcacc tgttagatgg aacagttggt gttgtggaac acggtctctt
      841 taatggtatg gttgataagg tgattgttgc tagtaaagac ggcgttactg ttttagaagt
      901 gcctaaagca agctagtctt agcatataac agtgttaatg tattttatag gtattgggga
      961 gtcaaccgta acctccccta gattggagac ttatgtcaaa atttaatcgt attcacttgg
     1021 ttgttttaga ctctgtcggt attggtgcag caccagatgc tgacaaattc tttaatgctg
     1081 gtgtagctga tacagattct gacaccctcg gtcatatttc agaaacagca ggcttatcgg
     1141 ttccaaatat ggcaaaaatt ggacttggaa acatttctcg tccaatacca ctaaaaactg
     1201 ttccaacaga agataatcct actggttacg tgaccaaatt ggaagaagta tctcttggaa
     1261 aagataccat gacaggccat tgggaaatta tgggtttgaa cattacagaa ccttttgaca
     1321 ctttctggaa tggtttccca gaagagattt taactaagat tgaagaattc tcaggtcgca
     1381 aaattattcg tgaagctaat aaaccctatt caggaactgc ggttattgat gattttggtt
     1441 cgcgtcaaat ggaaacaggt gagttgattg tttacacctc agcagaccca gtgcttcaaa
     1501 ttgcagccca tgaagacatt attcctgtgg aagagcttta caagatttgt gaatatgctc
     1561 gctccatcac gcttgaacgc cctgcccttt taggccgtat tattgcgcgt ccttatgtag
     1621 gcgaaccagg aaactttaca cgtacggcta accgtcatga ctatgcagtg tctcctttcc
     1681 aagataccgt tttgaacaaa ctggctgacg caggtgtccc aacttatgcc gttggtaaga
     1741 ttaatgacat ctttaatggt tcaggtatta caaacgacat gggacacaat aagtcgaaca
     1801 gccatggtat tgacacgctt attaagactc ttcaattacc tgagtttact aagggattct
     1861 cctttactaa cctcgttgat tttgatgcca actttggtca tcgtcgtgac ccagagggtt
     1921 atcgcgactg cttgcatgaa tttgacaatc gcctacctga aatcatcgca aatatgaaag
     1981 aagacgattt gcttctcatc actgctgacc atggtaatga cccaacctat gcaggcaccg
     2041 accacactcg tgaatacatt ccattgcttg cctactctgc atcctttact ggaaatggtc
     2101 ttattccaca agggcatttt gcagatattt cagcgactgt tgcagaaaat tttggagttg
     2161 atatagccat gattggggag tcattccttg gtcacttaaa ataagaaaat tagttgataa
     2221 tggaagaaat cacgatttat cataatccaa actgtggcac ttcacgtaat gttcttgcca
     2281 tgattcgcca tgcaggtatt gagcctacta ttattgagta tttgcagacg ccacctaatc
     2341 gggagacttt aattgagctc ttacaatcga tgggaatctc tgcgcgagaa ctcttgcgaa
     2401 ccaatgttcc tgaatttgag gctcatggat tagcaaatca agcagtagct gagaaagacg
     2461 ttattaatgc catgttgacg gatccaattt tgatcaatcg tcctattgtt gtgactcgca
     2521 aaggagtaaa actttgccgt ccttctgaga ccttactgga tattcttcct gttcccctgc
     2581 ctagtcctta tattaaagag gatggcgaga ttgtgaaccc tattgaggta aaaggagaaa
     2641 aagatgtcat tgatgactaa aattaacgaa acaaaagact ttttagttgc taaaggaatt
     2701 gaagctccag aatttggatt aattctgggt tcaggacttg gtgaacttgc tgaagaagta
     2761 gagaatcctg ttgttattga ttatgcagat attccaaact ggggcaaatc aacagttgtt
     2821 ggccatgctg gaaaattagt ctacggtgat ttagctggtc gtaaagtact tgccctacaa
     2881 ggtcgtttcc atttttacga aggaaacccg cttgaagtgg tgactttccc tgttcgtgtc
     2941 atgaaagctc tgggatgtga agg
//
LOCUS       DAUVUY010000132         2960 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.133, whole genome shotgun sequence.
ACCESSION   DAUVUY010000132 DAUVUY010000000
VERSION     DAUVUY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.133"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..507
                     /locus_tag="VOX58_001862"
     CDS             <1..507
                     /locus_tag="VOX58_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HES5879063.1"
                     /translation="KTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLH
                     MILKELTDKSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQT
                     STELQLKMIDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLP
                     IVKGDDDE"
     gene            500..1141
                     /locus_tag="VOX58_001863"
     CDS             500..1141
                     /locus_tag="VOX58_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HES5879064.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            1178..2578
                     /locus_tag="VOX58_001864"
     CDS             1178..2578
                     /locus_tag="VOX58_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HES5879065.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPLLQEKADFITKKVKEDGILYALEELGLIDKELQFPQL
                     DLPNHKGPKATIKTNHGDMTLVLFPDHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPAPIAASYAAIGGTPHLDRRHTVFGQLVDETSFQVLDLIAGVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            2578..2955
                     /locus_tag="VOX58_001865"
     CDS             2578..2955
                     /locus_tag="VOX58_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HES5879066.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALENGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKPSLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
BASE COUNT          953 a          492 c          664 g          851 t
ORIGIN      
        1 aaaacacaat tacaaacaca gttaacaacg gttgaagcaa tgttgcaaaa tgctcaaaat
       61 gatctacgca ttctcctttt gcatcttaga cctaccgagc tagctaatcg gactttatct
      121 gagggccttc atatgattct taaggaatta acagataaaa gtgatattga agtcatttat
      181 aaggaaacca ttgctcagct tcctaaaaca atggaagata atctttttag aattgcccaa
      241 gaattcatta gcaacacgtt aaaacatgct aaagctagtc gaattgaagt ttatctcaat
      301 caaacctcaa cagaattaca attgaagatg attgatgatg gtgtaggatt tgatatggat
      361 caggtaaggg atttgagtta tggtctgaag aatattgaag accgtgtcaa tgatttagca
      421 ggaaacctac atttaattag tcaaaaaggc aaaggagttt ccatggatat tagactgccg
      481 atagtgaagg gagatgacga tgagtaagat aaaagtgata ttggtcgatg atcatgaaat
      541 ggtccgcatg ggactcaaga gttttttgaa tttacaagct gatattgatg tcgttggtga
      601 ggcctctaat ggacgtgaag gggttgattt ggcattggct ttgaagccag atgttttggt
      661 tatggatcta gtgatgccag agttaggcgg tgttgaggca actttagaag ttctaaaaaa
      721 atggaaagag gctaaggtac ttgtgttaac ttcctatcta gataatgaaa agatataccc
      781 tgtcattgat gcaggagcga agggttatat gttaaaaaca tcgagtgcgg ctgaaatttt
      841 aaatgccatt cgcaaggttt caaagggaga gttagctatt gaaacagaag ttgacaaaaa
      901 aattaaggcg catgatcaac accctgactt gcatgaggaa ctaacagcgc gtgagtatga
      961 tattttacac cttttagcta aagggtatga taatcagacc atcgctgatg aactctttat
     1021 ttccttaaaa accgtcaaaa cacatgtgtc caatattcta gccaagttag aagttgatga
     1081 ccgaacccaa gcggttgtct atgcttttcg acatcattta gtcccccaag acgataatta
     1141 atgctataat atagggtata gcaaattgga ggaaagtatg gacgcaaaac taaaatacaa
     1201 agctaaaaaa atcaaaatgg tgttctttga tattgatgat accttgaggg tcaaagatac
     1261 gggttatatg cctgagtcga ttcaaagagt atttaaggct ttgaaggcaa aaggcatctt
     1321 ggttggtatt gcttcaggtc gcgctcggta cggtgttcca caagaagttc aagatttaca
     1381 tgctgactat tgtgtcaaat taaatggagc ttatgtcaaa gacgatgcta aaactattat
     1441 tttccaagct ccgattccag cagatgttgt tgtggcctat aaaaaatggg cggatgatat
     1501 gggcattttt tatggaatgg cgggtcgtca tgaagcggtg ttgtccgctc gaaatgacat
     1561 gattagcaat gctattgata atgtctacgc tcaactagag gtctgtccgg attataatga
     1621 gtatcatgat gtttaccaga tgtggacttt cgaggacaag ggagatggct tgcagttgcc
     1681 tgctgaatta gcagagcacc ttcgtttggt cagatggcac gataactcat cagatgttgt
     1741 cttgaaaggt acgtcaaaag cgctaggtgt ttcaaaagta gttgaccatt taggattaaa
     1801 acctgaaaac attttggttt ttggagatga actcaatgac ctagagttat ttgactatgc
     1861 tggtatcagt attgcaatgg gagtatcaca tccattactg caagaaaaag cagattttat
     1921 cacaaaaaaa gttaaagaag atggcatttt atatgccttg gaggaattag gattgattga
     1981 caaagaatta cagtttccac aattggattt gccaaaccat aaaggaccaa aagcaaccat
     2041 taaaacaaac catggcgata tgaccctggt cttgttccca gatcatgctc ctaaaacagt
     2101 agccaatttc ttaggtttgg ctaaagaagg gtactatgat gggattatct tccaccgtat
     2161 tattcctgaa tttatgattc aagggggcga tccaacagga acaggaatgg gtggacaatc
     2221 catctatggt gaaagttttg aagatgaatt ttcggatgaa ctctataatc tccgtggggc
     2281 cttgtcgatg gctaatgcgg gaccaaatac taacggcagt cagttcttta ttgttcaaaa
     2341 tagtaagatt ccttatgcca aaaaagaact agaacgtggt ggttggccgg ctccaattgc
     2401 agcttcttac gctgcaattg gtggaactcc tcaccttgat cgtcgtcata cggtctttgg
     2461 tcagcttgtg gacgaaactt cattccaagt tttggatttg attgctggcg ttgaaactgg
     2521 cgcacaggat aaacctaaag aagacgttat cattgaaaca atcgaggtat ttgactaatg
     2581 aaaattggcg acaaactgca tggcaccatc actgggatta aaccatacgg agcctttgtt
     2641 gcccttgaaa atgggacaac aggtctcatt catatttcag aaataaaaac gggatttatt
     2701 gacgatattg atcaattatt ggctataggc aaccaggttt tagtacaagt aattgatatt
     2761 gatgaatata gtaaaaagcc aagtttatct atgcggacct tagcagaaga aaaacagcat
     2821 tttttccatc gtcatcgtta ttccaacagt cggcacaaga ttggctttag gccacttgaa
     2881 gagcagttac ctcagtggat tgaagaaagc ctgcagtttt taaaaaagaa cactaaaaag
     2941 agtctggaac cttagtagtt
//
LOCUS       DAUVUY010000133         2903 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.134, whole genome shotgun sequence.
ACCESSION   DAUVUY010000133 DAUVUY010000000
VERSION     DAUVUY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.134"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..312
                     /locus_tag="VOX58_001866"
     CDS             <1..312
                     /locus_tag="VOX58_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HES5879067.1"
                     /translation="NLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPEAVLEA
                     AVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVEAVKTV
                     L"
     gene            486..1745
                     /locus_tag="VOX58_001867"
     CDS             486..1745
                     /locus_tag="VOX58_001867"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HES5879068.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            2103..2840
                     /locus_tag="VOX58_001868"
     CDS             2103..2840
                     /locus_tag="VOX58_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HES5879069.1"
                     /translation="MGKEIKVKYFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALVDQA
                     DALQSEEAAVVQSDTAASDALEALADQTDALQSEEAAVVKADTAASDALEALADQTDA
                     VQSEEASVVKADTAASDAWEKAATPTALDVKKTKDTTPVVKKEERQNVNTLPPTGEES
                     NPFFTAAALAITVSTGALVVSSKCKEN"
BASE COUNT          947 a          472 c          625 g          859 t
ORIGIN      
        1 aaccttgtgg cagcaggtct tgcgacgaaa gctgaagtcc aactagctta tgcgattggg
       61 gtggctcagc ctgtgtctgt tcgcgtggat acatttggaa cctcaacggt tcctgaagct
      121 gttttagaag ctgctgttcg tcaagtcttt gatttacggc cagcaggcat tattcaaatg
      181 cttgatttaa aacgcccgat ttacaaacaa acagctgcct atggtcacat ggggagaaca
      241 gatattgatt tgccatggga gcgtttaaat aaggttgatg ctttagtaga agctgtaaaa
      301 acagtattat aaacgacaac tttattaaaa aagtagagtg agtgaaaaaa tcttactctg
      361 cttttttgtt attaaataag cctttaaaag ggatgattaa aaattaagac gtcaataatt
      421 gtcaggcttc atgatttatg ataaaatgaa aaggttgaat tctagtataa gaaagttttg
      481 aatgtatgcg taaaattatt attaatggcg gaaaagcctt atctggagaa gtggcagttt
      541 caggggcaaa aaacagtgtt gttgcgctta ttccagcaat catcttagcc gatgatattg
      601 tgattcttga tggtgtacct gctattagtg atgtagatag tctgattgaa atcatggagc
      661 ttatgggagc aacagtaaac tatcatggtg atactttaga aattgaccct agaggggtgc
      721 aagacatccc gatgccttac ggaaagatta atagtcttcg agcttcctat tatttctacg
      781 gtagtctttt aggtcgtttt ggacaagcag ttgttggctt acctggtggt tgcgatttag
      841 gaccaagacc aattgacttg catttgaagg ccttcgaggc tatgggagtt gaagtgtctt
      901 atgagggtga aaatatgaat ttatcgacaa atggtcaaaa aattcatggc gctcatatct
      961 atatggacac cgttagtgtc ggtgctacaa ttaatactat ggttgcggca acaaaagctc
     1021 aaggtaaaac ggttattgaa aacgctgcac gtgagccaga gattattgat gtggctactc
     1081 ttttgaataa catgggtgcg catattcgtg gagcaggtac agatattatt acgatacaag
     1141 gtgttcagaa attgcatggt actagacacc aagttattcc tgatcgtatt gaagcaggga
     1201 cttatatcgc tttggcggca gctattggaa aaggtgttaa gatcactaat gttctttatg
     1261 agcatttaga gagtttcatt gctaagttag aagaaatggg tgtccgtatg acggtagaag
     1321 aggatgctat ctttgttgaa aagcaagaat ccctcaaggc tattacaatt aaaacctctc
     1381 cttaccctgg ttttgccact gatttgcaac agccattaac gcccttgttg ttaaaagcag
     1441 atggtcgtgg aacgattatt gacactatat atgaaaagcg cattaaccat gtgccagagt
     1501 tgatgcgcat gggagcagat atctcagtta ttggtggtca gattgtttat caagggccgt
     1561 ctcgcctgac aggagctcag gtcaaagcca ctgatttacg tgccggagcg gctctagtga
     1621 ctgctggtct aatagctgaa ggcaagacag aaattactaa tattgaattt atcttacgtg
     1681 gctatgctag tatcattgca aaattaacag cactaggggc agatattcaa ttaatcgaag
     1741 actaaagaga agctaacgct tctctttttt atattacatg tgcttaggct aataatactt
     1801 aggtgcatac tttgttgcat tttatggctt tttaactgtc ataaaaaata taaaaatgat
     1861 atcactaact aatattatta attttaatgc taaaataatg agttgacggt agtaaagtga
     1921 taaataaaca ctccttaaaa tcttatttta ttaatttttt taaaaaatat tatataaatt
     1981 agataataaa ctttatttta tgaaattaat ctgtataata aaagatggaa ttattaatta
     2041 taatttccaa attatcggta atttaatatg ctaatgcata taaaaataaa aaaggagaaa
     2101 caatgggaaa agaaataaaa gtgaaatact ttttgcgtag atcagctttt ggattagttg
     2161 cggtgtcagc atcagtatta gtcggttcaa cagtatctgc tgttgactca cctatcgaac
     2221 agcctcgaat tattccaaat ggcggaacct taactaatct tcttggcaat gctccagaaa
     2281 aactggcatt acgtaatgaa gaaagagcca ttgatgaatt aaaaaaacaa gctattgagg
     2341 ataaagaagc tacgacagct atagaagcag caagttcaga tgccttagaa gcattagtgg
     2401 atcaagcaga cgctttacaa tcagaagaag ctgcagtagt tcaatcagat accgctgcta
     2461 gtgatgcctt agaagcattg gcggatcaaa cagacgcttt acaatcagaa gaagctgcgg
     2521 ttgttaaagc ggataccgct gctagtgacg ccttagaagc attggcggat caaacagacg
     2581 ctgtacaatc agaagaagct tcagttgtta aagcagatac cgctgctagt gacgcctggg
     2641 aaaaagcagc aactccaacc gctttagatg ttaagaaaac taaagataca acacctgtag
     2701 ttaaaaaaga agaaagacaa aacgttaata cccttcctcc aactggtgaa gagtctaacc
     2761 cattctttac agctgctgcg cttgcaataa cggtaagtac aggtgcgtta gttgtaagtt
     2821 caaagtgcaa agaaaattag ttgctatttg ttctagcaaa tgaaacaaga gaatcgaaag
     2881 attctctttt tctgatattt aaa
//
LOCUS       DAUVUY010000134         2774 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.135, whole genome shotgun sequence.
ACCESSION   DAUVUY010000134 DAUVUY010000000
VERSION     DAUVUY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.135"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..153
                     /locus_tag="VOX58_001869"
     CDS             <1..153
                     /locus_tag="VOX58_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HES5879070.1"
                     /translation="IPISILIGMILLLVADTVGRVYLVGTNIQTGILVSLIGAPYFLY
                     LMAKTK"
     gene            complement(198..851)
                     /locus_tag="VOX58_001870"
     CDS             complement(198..851)
                     /locus_tag="VOX58_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HES5879071.1"
                     /translation="MITVFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVMIKEKTNITRDDLTLANYFYRLKRGEKPSAEQLDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(927..1862)
                     /locus_tag="VOX58_001871"
     CDS             complement(927..1862)
                     /locus_tag="VOX58_001871"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HES5879072.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            complement(1993..>2774)
                     /gene="pflA"
                     /locus_tag="VOX58_001872"
     CDS             complement(1993..>2774)
                     /gene="pflA"
                     /locus_tag="VOX58_001872"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HES5879073.1"
                     /translation="DYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCHNPDT
                     WEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEAKKLG
                     IHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKNILQF
                     ARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGEFKWR
                     ELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
BASE COUNT          828 a          571 c          504 g          871 t
ORIGIN      
        1 atacccatta gtattttaat cggaatgatc ctacttcttg ttgcagatac agtcgggcga
       61 gtgtatctgg ttggcactaa tatccagaca gggattttag tgtcgcttat cggagcccct
      121 tattttcttt acttaatggc aaaaacaaaa taaaagcact caaaaagcta agaacagtcc
      181 ttgttcctag cttttttcta tggtgctata aaggttaaac tttctttatc caaggtcatt
      241 agcagctggt atgttgtagg ctccacttgt tttaggtaac caagtgtaac cagagcttcc
      301 acaaatatat caggacgttt ttgcatcaca aggtctttgc gcgtgaattt aagcaaaaaa
      361 gttgtcatat atttaagggc atattcctga ttcacatccc ccaagaggtc atacaagtcc
      421 agctgctcag ctgatggctt ctcacctctt ttgagacggt aaaaataatt agccagggtc
      481 aaatcgtctc tcgtgatgtt ggtcttttcc ttgatcatca cgtgattggt ttggtttgtt
      541 agctgagtct caaaaacaac cgctaaaata ttttcataca tagctgaaca ggtgtccaca
      601 aaaagcatag tgtctaaggc cagctgctga tcagaactca ccaaaggaag attgattcca
      661 taacgcttat tttcacggcg aatatagcca gcttgaacat agctttcgat ggccttgtca
      721 atacctgtca cattaggaaa ggcttttttg atctctcgta atataacgtg atcatgctgg
      781 tcgagataat tgataaggtc ttgaaagaaa ggttgtctgg tgagtttatc tgaatgaaat
      841 actgttatca taccttctat tataacatag aagagtgaca aaaggacttg gtcaaaaaaa
      901 tcaagtcctt ttaaagcgtc ttttcattaa gctccaaaac tttctgtcaa ttgtggaacc
      961 acttgttttt tacgagaaac ggcacctgct aagaaggcat gattgttgtc aagggtgaag
     1021 ttaaaggctg cttcaacctt gtccatgttt gctccaatag ctagaatttc agagtttgag
     1081 ttaacaatat ccgtgatcat caaaacaaaa tctgaataac cttctgcagc catggcatct
     1141 ttaatggctg cttcaatagc ttcttgacgt tctaagactt ctgcaatgtc aacagtgttc
     1201 acttgtgcca cgcgcacagc attgccattt aattcaaatg ttttggcatc aataccaatc
     1261 aattctactt ctgatttgct tgcaaggttt gtacctgctt ttaaaagagc cattccatag
     1321 tcttccaagt tcacttctgc tagctctgcc aattcttcag cgacaaggtg atcagacaca
     1381 tgagttgttg gagatttcag caacaaggta tctgaaatca aaccagacaa gagcatccct
     1441 gcaatcgctt ttggcacctc gatgccattt tctttaaaca tacggtaaac aatagatgag
     1501 gctgatccta ctggttccac acgcatgtat aaaggatttg cagtttcaaa attagccaca
     1561 cggtggtgat caaccacacc atatacctca acctcacgga tatctgcaat ggattgttgg
     1621 aattcattgt ggtctgtcaa aatcacttgt tcagaccctt gtgctttagc agactctacc
     1681 acacgtggtg cctcaacacc aaaataatca agggcaaaag ctgtttcttc gttaggtgtt
     1741 cctaaagcta cgacttcagt atccaaacca aacgcttttt gggataagta atcaaaagca
     1801 taagatgatg caatagcatc cgtatcagga ttttgatgac caaaaactaa aatttttgac
     1861 ataatcattc tacctcattt tgtttattca ttttatttta gcaaaattac cccctaaaat
     1921 caaaggaaga ttacaaaaga tctgcttata atactttaaa aagttgagga aacctcaacc
     1981 ctataacagc tcttatgact gatgaatccg attcatatat tctgtgtaag attcggtctg
     2041 catcagattt ttagcatttt gaacgcgttc cttagttggt ggtttaacac cttctaactg
     2101 ataaggaata cctagctcac gccacttgaa ttctcccatg gtatggtaag gcaaaacttc
     2161 aaatttatcg acatttttca aggttttgac aaactcaccc aatcgtgtca aatgatcatc
     2221 aatatcagtc aagcctggaa ccaagacatg tcgaatccag acaggaatct gcttgtcgga
     2281 taaataccta gcaaattgca atatgttttt attaggctga cgcgtgacaa ttttatgttg
     2341 tttttcatca atttctttta agtccaataa aatcaaatcg gtcactgcca ataagttatc
     2401 aagaacctga tggtattcag gtgtcggtct ataggtaaag ccacaggtgt ctagtgtggt
     2461 atggatacct aattttttag cttcgatgaa taaggcagtg ataaaatcaa tttgaagcat
     2521 agcttcacca ccagatacgg taattcctcc tttttttccc cagaaatgtt tgtactgaag
     2581 ggcttctttc aagacatcat tgactgttct gatttttgaa ttgttggttt ccatctccca
     2641 agtatccgga ttatggcaat attggcaacg taatttacag ccttgcaaga aaatgataaa
     2701 acgaatgcct ggaccatcta cagaaccaaa actttctgtc gaatgcacca tccctgttac
     2761 ttgtccataa tctt
//
LOCUS       DAUVUY010000135         2769 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.136, whole genome shotgun sequence.
ACCESSION   DAUVUY010000135 DAUVUY010000000
VERSION     DAUVUY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.136"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..570)
                     /gene="pheS"
                     /locus_tag="VOX58_001873"
     CDS             complement(<1..570)
                     /gene="pheS"
                     /locus_tag="VOX58_001873"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HES5879074.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSK"
     gene            complement(853..1717)
                     /locus_tag="VOX58_001874"
                     /pseudo
     CDS             complement(853..1717)
                     /locus_tag="VOX58_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            complement(1753..1941)
                     /locus_tag="VOX58_001875"
     CDS             complement(1753..1941)
                     /locus_tag="VOX58_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HES5879075.1"
                     /translation="MTNGLKYVLEQMLLLLVIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            complement(1945..>2769)
                     /locus_tag="VOX58_001876"
     CDS             complement(1945..>2769)
                     /locus_tag="VOX58_001876"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HES5879076.1"
                     /translation="AQADRLQGAMIYMDFPSVGATQNLMMAATLADGVTTIENAAREP
                     EIVDLAQFLNKMGARIRGAGTETLTITGVTSLHGVEHDVVQDRIEAGTFMVAAAMTSG
                     NVLIRDAVWEHNRPLISKLMEMGVSVTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTD
                     MQAQFTALMAVVNGESTMVETVFENRFQHLEEMRRMGLQSEILRETAMIHGGRQLQGA
                     PVMSTDLRASAALILTGIVAQGVTIVNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
BASE COUNT          785 a          627 c          508 g          849 t
ORIGIN      
        1 ttttgaaaaa tcatgttgat caagagtacg ggcctgcaca ggactggtgt gagttcgtaa
       61 caagatttct tctgtgatat aaaacgtatc ttgcatgtca cgggcagggt gatcctttgg
      121 caaattcatg cgctcaaagt tgtaataatc tttttctacc tcaaagccat caacaatctg
      181 aaaacccatt cctaagaaaa tatcttcgat ttcttcactg gtttgtgtta acacatgacg
      241 gtggcccaga gtcatttgac gacccggtaa agtcacatca atgctttcag catcaagctg
      301 ggcttgaatt ttagcagctt ctacaatttt agcctgttct tcaaaggctt ttgtcaataa
      361 gtcacgaact tcgttcacct gtttaccaac cacaggacgc aagtcattag acaagtcttt
      421 tagccccttt agtaattctg tcaaagatcc ctttttccca agaacagcca cgcgcaaatc
      481 ttgcagttct ttggtatgat tccccgtcaa agattggaga gtctccaatg tttttgtttt
      541 taattcctct aattgtgctt gtaaatccat agttttccct tcttttagcg atgaaaaaag
      601 cgtgccacta ctccacttca taagcggagc gttgacacgc ggtaccatcc gtttttatct
      661 gatgttatca gaccttaatt aattgtcttt agctgagtga attcctaata gttcccttaa
      721 gaaggcttcc agtcacggcc ctcactccct gttaatttct ctattagtac tagtctcata
      781 gacacttatt caattagttc tattctatca aaaaaagtgc ttagtgacaa tcttaaatca
      841 gtgacttttc gcttagagat tgatagctat ttgtcccgtc cgatagtcaa cggtcaatcc
      901 tggttggaca tttggaacaa acacgttaaa agtcaagctg tcatcagagg atttagcctc
      961 caaatggcta ccgctggcta gtagattatc accatcataa agtagggtga cacggtagcg
     1021 gacgcgatgc cccttatcca aagcacgacg tactaaacct tcatagtagt tctgacctgt
     1081 cgagtagggt ttatttactt gattagccca acttagttga gtggcaatat tatcaggatt
     1141 actggtaagc atcaaaacct ttcagtccac caactaaagc ataacctagc aaatgccctc
     1201 tatcaaccgc gtgatcataa cttcctttta agtggtgcaa tcggtgccaa cctgctggct
     1261 taaaggtacg ataagcattg ttcgtttgat agcgattttg atactgtcgt gtcgacttag
     1321 taagtaaggc attagctcta gtaggaacct tttgctcttg cacccatttg gtttcattgt
     1381 tggcataagg cggactggct accttagcag ttaaatctgt tttgttcctg ttgataatat
     1441 aggcgcctgc tccattccac tgaacatctg gacctagttg ctttttgatt gatggtgtca
     1501 ataccgatgc cgccagagct tctgtgggaa catcagcggg caaattatta tgtgtgagta
     1561 atgatttttt cttctcccca acaagagtct gataaagttg cttgataggg tgagtgctag
     1621 ctatgaaatc actcttagca aggctgttag accccaacac taacaaaatc aataagaagg
     1681 aattaagcat cctttttcgt tttttccttg ttaacatact tcctctcaaa tgctataaaa
     1741 agtaggcaag gcctactttc cagtaaattt attcactaac tctgcccact tatccataga
     1801 taaaatatgc catggactat ggccatctcc cataaaacta taaccaatca taagtccaat
     1861 ggctaaaaaa agacacgcca aggcagcaat tactaaaagt aataacattt gttctaagac
     1921 atattttaaa ccatttgtca taagctagac ctcactgcta cgactaatag tagctccaag
     1981 ctttgctaat ttttcatgaa attgatagta gccacgatct aaatgaacca aattattaac
     2041 tattgtcact ccttgagcaa caatccctgt caagataagg gcagcactgg cacgtaaatc
     2101 tgttgacatc acaggagcac cttgcagttg tcttccacca tgaatcatgg cagtttccct
     2161 aaggatttca gattgtaagc ccatgcgtcg catttcttcc agatgttgaa atcgattttc
     2221 aaagacagtt tcaaccatag tagactctcc attgaccact gccattaaag ctgtaaattg
     2281 agcttgcatg tctgttggaa aacctggatg aggcaaggtt ttaaccgtta caggtttgag
     2341 ctttggcgtg tttgcttgaa cgcgaatacc atattcttct tctgttacag aaacacccat
     2401 ttccattaat tttgaaatca atggacgatt atgttcccaa acagcatctc taatcaaaac
     2461 attgcctgaa gtcatcgccg ctgcaaccat aaaagtacct gcttcaatac ggtcttgaac
     2521 cacatcgtgc tccaccccat gaagacttgt gacgcctgtt atcgttaacg tctctgttcc
     2581 agcaccacga attctagctc ccattttatt caaaaattga gctaaatcaa caatttcagg
     2641 ctcacgagca gcattttcaa tagtggtcac accatcagct aaagtggcag ccatcatcaa
     2701 gttttgggta gctcctacag atgggaagtc catataaatc atggcacctt gcagtcgatc
     2761 tgcttgagc
//
LOCUS       DAUVUY010000136         2713 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.137, whole genome shotgun sequence.
ACCESSION   DAUVUY010000136 DAUVUY010000000
VERSION     DAUVUY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.137"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1557
                     /locus_tag="VOX58_001877"
     CDS             <1..1557
                     /locus_tag="VOX58_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879077.1"
                     /translation="IYVAYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKI
                     NDFSKDVSTASLNIGTKIVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVE
                     ALGEERVESLLSTSPDTNNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFN
                     IGISIFVFLLTGIMIFSQVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTR
                     AGITLIITVAFSISTMLYSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDS
                     QNMGRQVFRRPRQMMNRQTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQN
                     ERTTPKKETALSNVKKQQGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVK
                     YALHQGKENVTHNVTDFKQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRR
                     KENPSSPVQNNQQHVRKRQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLN
                     KRTDKRGQQT"
     gene            1554..2576
                     /locus_tag="VOX58_001878"
     CDS             1554..2576
                     /locus_tag="VOX58_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HES5879078.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            2593..>2713
                     /locus_tag="VOX58_001879"
     CDS             2593..>2713
                     /locus_tag="VOX58_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HES5879079.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFV"
BASE COUNT          909 a          511 c          561 g          732 t
ORIGIN      
        1 atctatgttg cctatacagg attggttaag cgagaaacga cgaaagtgat acgagctgtt
       61 ttaaactttg tcgtggtgtt tattctatcg gcttccttta ttgcgtatgc acccgattat
      121 attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg ccagtctgaa tattgggaca
      181 aaaattgttc ttcctaacac ggatagtcaa gggaaagaca gtgtggacat gatacgtgac
      241 agcttattct ctgttcaagt aaagcaacca tggctattgc ttcaatatgg ctccactgat
      301 gtagaagcgt taggggaaga acgtgtggaa agtttattgt ctaccagtcc agatacaaac
      361 aatggtgaag atagagaaaa tgtagtgatt gaagaaattg aagatcatga caatacctat
      421 ttaaccctac cgaagacgat ttccagatta ggaacagtct tctttttgtt tgtattcaat
      481 attggtatct cgatttttgt tttccttcta acaggaatca tgattttttc gcaagtgcta
      541 tttattattt tcgctatgtt tttgccaatc agtttcctat taagtatgtt gccgagtttt
      601 gatggcatgg gcaggcatgc cattaccaag ttatttaatg tgattatgac aagggctggt
      661 attaccttga ttattaccgt tgcctttagt atttccacta tgctttattc gctgtcagca
      721 agttctccct ttttcatgat tatgttttta caaattgtga cctttgcagg gatttatttt
      781 aaactgggcg atttaatgag cctattcact ttacaaagca acgactctca aaacatgggc
      841 agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac aaaccagaag atttcaacgc
      901 agtatgagta agatttttcg tggaaaagca gggtcaacga agcaaaagac aatgacaaca
      961 aagcagaatc aaaccaataa agctaaccat actcgtcaaa atgaacggac tacaccaaag
     1021 aaagaaacag cactttccaa tgtgaaaaag caacaaggcg gtatcgggca acgattaggt
     1081 gaaaagacag cccatgtatt ggacacgaaa aatcgtctag tcaataaaag aaagcaggca
     1141 aaacaacaga tacaggcgac accaacccat gtcaaatatg cccttcatca aggaaaagag
     1201 aatgtcactc ataatgtaac agactttaaa caatctatgg cagataccaa agctcaacgc
     1261 aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga acattgctca aaagcgtttg
     1321 gagctggata aagcaaaaga aaaaagacga aaagaaaacc catcatctcc tgttcagaat
     1381 aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc ctgttttgtc acaaaacact
     1441 aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa aacaaaagac accaccgaag
     1501 caattcaatc agaggaagct aaataaaaga acggataagc gaggacaaca aacatgaagc
     1561 taaaaataac agtgatttgt agtgtggtct tctctctctt cttttttctc ttattattcc
     1621 tagtcatttt ctttgcagat gatacggaca gtggcgaaaa caacaaggat tcttctattc
     1681 cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca ccgtccgttg attgaaaaat
     1741 atggtaagga atatgggatt gaagactatg tttcttatat cttagccatt atgcaagtgg
     1801 aatcgggcgg tacagcagaa gatgttatgc aaagttccga atctttgggt ttaccgccca
     1861 acagtttgag taccgaagag tccatcaaac aaggggttaa atatttcagt gaattattaa
     1921 ccagtgcaga acaacaagga gtggatatag attctgttat ccaatcttat aattatggcg
     1981 gtggcttttt aaattatgtg aggagtcatg ggaaaaaata tacctatgaa ttagccgaac
     2041 agttttctaa agagaagtcg ggtggtcaaa aagcagacta tcccaatcct atagccatac
     2101 ctgtgaatgg tggttggcga tataactatg gaaatcagtt ttatgttcag ttggtttcac
     2161 aatacctaac cgacacttcc ccaacagaat ttgatgatga aaccgtgcaa gtcattatgg
     2221 acgaagcctt gaaatacgaa gggtttccct atgtctttgg aggggcgtcg cctaccacct
     2281 cttttgattg tagtggctta atacaatggg tgtatgacaa agctggcatt tctctccctc
     2341 gtgtcgccca agatcagtat gacgctacgc aagaaatctc tatggaagaa gcccaagcag
     2401 gcgaccttat cttctttcat tcaacctata atgcaggcac atatgtgact catgtggcga
     2461 tatatttaga gggaaatcgc ttttatcatg caggcgaccc aatcggttat ggcgacttaa
     2521 gcagtcgtta ttggcaagac catctgattg gggctagacg tgttattcat aactaagaaa
     2581 ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa agaaggagaa accaaagaaa
     2641 tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta aagttggcac gcataagaaa
     2701 ttaacctttg ttt
//
LOCUS       DAUVUY010000137         2633 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.138, whole genome shotgun sequence.
ACCESSION   DAUVUY010000137 DAUVUY010000000
VERSION     DAUVUY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.138"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..863
                     /gene="ptsP"
                     /locus_tag="VOX58_001880"
     CDS             <1..863
                     /gene="ptsP"
                     /locus_tag="VOX58_001880"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HES5879080.1"
                     /translation="EAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRT
                     MDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRIM
                     FPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVD
                     FFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGEM
                     AGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEVL
                     ELSKEYVSED"
     gene            1048..2475
                     /locus_tag="VOX58_001881"
     CDS             1048..2475
                     /locus_tag="VOX58_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HES5879081.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALISGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLVLAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
BASE COUNT          785 a          477 c          577 g          794 t
ORIGIN      
        1 ctgaagctgt tggcctttac cgtactgagt tcttgtacat ggattctcaa gacttcccaa
       61 ctgaagacga acaatacgaa gcttacaaag cagtgcttga aggcatgaat ggcaaacctg
      121 ttgtggttcg tacaatggat attggtggag ataaggaact tccttacttt gaccttccaa
      181 aagaaatgaa cccattcctc ggtttccgtg ctcttcgtat ctccatctct gaaactgggg
      241 atgccatgtt ccgcacacaa atgcgtgcgc ttcttcgcgc ctctgttcac ggacaacttc
      301 gtatcatgtt cccaatggta gcacttctta aagaattccg tgctgcaaaa gcagtctttg
      361 acgaagaaaa agcaaacttg cttgcagaag gcgttgcggt tgctgatgac atccaagttg
      421 gtatcatgat tgagattcct gcagctgcta tgcttgcaga ccaatttgct aaggaagttg
      481 atttcttctc aattggaaca aacgacctta tccaatacac tatggcagca gaccgtatga
      541 acgaacaagt atcatacctt taccaaccat acaacccatc aatattacgt ttgatcaaca
      601 atgtgatcaa agcagcgcac gctgaaggta aatgggcagg tatgtgtggt gagatggcag
      661 gtgaccaaca agctgttcca cttcttgttg gaatgggctt ggatgagttt tctatgtcag
      721 caacttcagt acttcgtacg cgtagtttaa tgaagaaact tgactctgct aagatggaag
      781 aatatgcaaa tcgtgcgctt acagaatgtt caacagcaga agaagttctt gaactttcta
      841 aagaatacgt ttctgaagat taacgggtta aagagttggt ttaaaccagc tcttttttgc
      901 tagctaaaaa tacaattact ttttggtgtg aaatcatttg agttatcaaa taattatcag
      961 aattttcaga tatttagtgt agccaaatct tgaattatat cagaaaagtg atagaataag
     1021 attactatca taaaaggaga taaaacgttg gcaaaacaat ataaaaattt agtgaacggt
     1081 gaatggaaac tatcagaaaa cgagattacc atttacgcac cagcaacagg tgaagagtta
     1141 ggatcagttc cagcgatgac gcaggcagag gtagatgctg tttacgcttc agctaaaaag
     1201 gctctatcag attggcgcgc cttgtcttat gtggaacgtg cagcttacct tcataaagcg
     1261 gctgatattt tagtacgtga tgctgaaaag atcggcgcga ttctttcaaa agaagtagcc
     1321 aaaggtcaca aggcagctgt cagtgaagtt attcgtaccg ctgaaatcat taattatgca
     1381 gcagaagaag ggcttcgtat ggaaggtgaa gttcttgaag gtggtagctt cgaagccgca
     1441 agtaagaaga agattgctat tgttcgtcgt gaaccagttg gtttagttct tgccatctca
     1501 ccttttaatt atcccgttaa cttggcaggt tctaaaattg ctccagccct tatttcagga
     1561 aatgttgttg ctcttaaacc accaacacaa ggctctattt ctggtttgtt actagcagaa
     1621 gcttttgcag aagctggtat tccagcaggt gtctttaata ccattacagg gcgaggttct
     1681 gttattgggg attacatcgt tgagcacgaa gcggttaact ttatcaactt tacaggttct
     1741 actccaattg gggaaggaat cggtaaatta gcgggtatgc gaccaattat gcttgagctt
     1801 ggcggtaagg attctgctat cgttttggaa gatgcagatc ttgttttagc agcgaaaaat
     1861 attgtagccg gtgcttttgg ttactcagga caacgttgta cagcggttaa acgcgttctt
     1921 gtgatggaca aggtggcgga tcaattggcg gctgagatta aaacacttgt tgaaaaacta
     1981 agtgtcggaa tgcctgaaga cgatgctgat attacaccat taattgatac atcagctgct
     2041 gattttgttg aagggttgat taaagatgca actgataagg gagctactgc tttgacagcc
     2101 tttaatcgtg aaggcaatct tatttcaccc gttctctttg atcatgtgac aactgacatg
     2161 cgtttggcat gggaagagcc gttcggccca gtattaccaa ttattcgtgt aaccactgta
     2221 gaagaagcca tcaagatttc taatgagtct gaatatggtt tgcaagcttc tatttttaca
     2281 actaatttcc caaaagcttt tggcattgct gagcaattag aagttggaac tgttcacctt
     2341 aacaataaaa cacaacgtgg aacagataat ttcccattct taggcgctaa aaaatcaggt
     2401 gcaggggtac aaggagttaa atattctatc gaagctatga caactgttaa atctgttgta
     2461 tttgatatcc agtaataaaa aaaataaaca aaaagttagg ttaacttaac tttttgttta
     2521 tttttttaat gttttttgtt acaatagtct atacagatgg aggcaactat tgaaaaaatt
     2581 aaatcttatt cttgttggtt tattaagcat tctgattttg agtttagcta ttg
//
LOCUS       DAUVUY010000138         2603 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.139, whole genome shotgun sequence.
ACCESSION   DAUVUY010000138 DAUVUY010000000
VERSION     DAUVUY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.139"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            97..987
                     /locus_tag="VOX58_001882"
     CDS             97..987
                     /locus_tag="VOX58_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HES5879082.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFATKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            1217..2071
                     /locus_tag="VOX58_001883"
     CDS             1217..2071
                     /locus_tag="VOX58_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HES5879083.1"
                     /translation="MKSKKVVSVISLTLSLFLVIGCAKVDNNKSVNCKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
     gene            2264..>2603
                     /gene="pgk"
                     /locus_tag="VOX58_001884"
     CDS             2264..>2603
                     /gene="pgk"
                     /locus_tag="VOX58_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HES5879084.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVL"
BASE COUNT          901 a          424 c          517 g          761 t
ORIGIN      
        1 tttgacaaaa cctagaaaaa cattataatt gtattataac aataagtcaa gaactgaaaa
       61 cggttcagtt ctaagatatg acaaggagga gattttatgt taggaccatt tatctttatt
      121 gcttttgggg ttattgtaat cctagctatt gttgccagta ccctttatgt tgttcgtcaa
      181 caatctgttg ccatcgtgga acgttttggt aggtaccaaa aaacagccac gagtggtatt
      241 catgtccgcc ttccgtttgg gattgacaaa attgctgcgc gtgtgcagct gcgtctttta
      301 cagtcagaaa tcatcgttga aaccaaaacc aaggacaatg tgtttgtaac tttgaatgtt
      361 gccactcagt atcgtgttaa cgaacaaaat gtaacagatg cttactacaa attaatgaaa
      421 cctgaatctc aaatcaagtc ttatattgaa gatgccttgc ggtcatccgt gccgaagttg
      481 accttagacg aattgtttga gaaaaaagat gaaattgcct tggaagtaca acatcaagtg
      541 gcagaagaaa tgtcaaccta tggatatatt attgtcaaaa ccttgattac caaggtggag
      601 cctgatgctg aagtcaaaca gtcaatgaat gaaatcaatg cggctcaacg caaacgtgta
      661 gctgctcaag agttggcaaa cgcagataaa attaaaattg tgacagcagc agaagcagag
      721 gctgaaaaag atcgattgca cggggtaggg attgcccaac agcgtaaggc cattgtggat
      781 ggtttggcgg agtctattca agagcttaaa gaagcgaata tttccttaaa cgaagaacaa
      841 atcatgtcca ttttgttgac caaccaatac ctggataccc tcaatacctt tgcgacaaaa
      901 ggaaaccaaa ccctttttct accaaataca ccaagtgggg tagaggatat ccgtacacaa
      961 gttttatctg cccttaaaac aaaataacgt gtaaaagctt ggctaaaaag ttgctcaata
     1021 ctggatttag cagaaggcta tcataccttg tgaagtggca aacgacgaaa tgaatacatt
     1081 acttttattg gagaaatgaa agtaaaaaaa gcctttttca tgattaacaa catagtattg
     1141 attaaatgtt aagttgtatt gacttttaaa ttaaataagt ttaaaatcaa cttaataagc
     1201 aaaaaggaga ttttcaatga aatccaaaaa agttgttagt gttatatcac ttaccttatc
     1261 cctttttttg gtgataggtt gtgctaaagt tgataacaac aaatcagtta attgtaagcc
     1321 tgctactaaa caaacctata atagctatag tgatgaccaa ttaagatcgc gtgaaaatac
     1381 catgtctgtt ttatggtacc agcgagctgc ggaaactcaa gcgctttatt tacaggggta
     1441 tcagttagca acggatcgct taaaagaaca actcaataaa ccaacggata aaccttattc
     1501 aattgtatta gatattgacg aaacagttct tgataatagc ccttatcaag ctaaaaatgt
     1561 tttggaagga acaggattta cacctgaaag ctgggattat tgggtacaaa agaaagaagc
     1621 aaaaccggtt gctggtgcta aagacttttt gcaatttgca gaccaaaatg gtgttcaaat
     1681 ttactacatt tcagacagat caactactca agtagatgct acaatggaaa atctccaaaa
     1741 agaaggtatt ccagtacaag gtcgtgatca tcttctattc ttagaaaaag gcgtaaaatc
     1801 aaaggagagt cgtcgtcaaa aggtcaaaga aacaactaat gtaacgatgc tatttggtga
     1861 taatcttcta gattttgctg atttttcaaa aaaatctcaa gaagatagaa cagctttatt
     1921 atcagatttg caagaagagt ttggaagacg ctttatcatt ttccctaatc ctatgtatgg
     1981 ttcatgggaa ggtgccattt ataaaggtga aaagctggat gtgcttaagc aactagagga
     2041 acgccgtaaa agtttaaaaa gctttaaata aagtaattga aaaagaggtg caaaagaaaa
     2101 tgtaacctct ttttaagtac tattattttc tgaaaatagg gttaatgact gaaaaaaatg
     2161 ttgattttgt gaaatgatta ttttattatt ttcatatttt atctttatgc taggaattgt
     2221 ggtataatgg aggagtataa aaaatattaa ggagtctatt cacatggcta aattgactgt
     2281 taaagacgtt gatttgaaag gtaaaaaagt cctcgttcgt gttgacttta atgtgccttt
     2341 gaaagatggc gttatcacta acgacaaccg tatcactgcg gctcttccaa caatcaagta
     2401 tatcatcgaa caaggtggtc gtgctatcct cttctctcac cttggacgtg ttaaagaaga
     2461 agctgacaaa gaaggaaaat cacttgcacc ggtagctgct gatttagctg ctaaacttgg
     2521 tcaagatgtt gtattcccag gtgttactcg tggttcaaaa ttagaagaag caatcaatgc
     2581 tttggaagat ggacaagttc ttt
//
LOCUS       DAUVUY010000139         2600 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.140, whole genome shotgun sequence.
ACCESSION   DAUVUY010000139 DAUVUY010000000
VERSION     DAUVUY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.140"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            98..1768
                     /locus_tag="VOX58_001885"
     CDS             98..1768
                     /locus_tag="VOX58_001885"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HES5879085.1"
                     /translation="MKSDIEIAQSVALQPIIDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLLLVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFTEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            1853..>2600
                     /locus_tag="VOX58_001886"
     CDS             1853..>2600
                     /locus_tag="VOX58_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HES5879086.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKS"
BASE COUNT          799 a          445 c          566 g          790 t
ORIGIN      
        1 gattaaaaac gaacaataat tgttttaatt caaataaatg tatgtatgat atgaattgtg
       61 atataatgac gtcattacgt aaaattgagg agtcatcatg aaatcagata tcgaaattgc
      121 ccaaagtgtc gccctacaac caatcattga cattgttaaa aaggttggaa tagacggcga
      181 tgacattgag ctttatggca aatataaggc taaattgtct tttgagaaaa tgaaagctgt
      241 cgaagctaat gagcctggaa aattactctt ggtaactgct attaacccta ctccagcggg
      301 agaaggaaaa tcaaccatgt ctattggact ggcagatgcc ttaaatcaaa tgggaaaaaa
      361 aaccatgctt gccttacgtg aaccctccct tggtcctgtt atggggatta aaggcggggc
      421 agcaggtggt ggttatgccc aagtattgcc tatggaagac attaatcttc actttactgg
      481 tgacatgcat gccattacaa ccgctaataa tgcattatct gctttgattg acaatcattt
      541 gcagcaagga aacgatttag gaattgatcc tcgccgcatt atttggaagc gcgtgttgga
      601 ccttaatgat cgtgctcttc gtcaggtaat tgttggctta ggcagtccag tgaatggtgt
      661 gccacgcgaa gatgggtttg atatcacagt agcttctgaa atcatggcaa ttttatgttt
      721 ggcaactgac ttgaaagatc taaagaaacg tcttgctgat atcgtcgtgg cttataccta
      781 tgatcgtaag cctgtctatg tgcgtgactt gaaggttgaa ggagcattga cattaattct
      841 aaaagatgct atcaagccaa acttggtgca gactatttat ggaacaccag ctcttattca
      901 tggggggcca tttgctaata ttgctcatgg ttgtaattct gtccttgcta cctcaacagc
      961 tttgcgatta gcagattaca ctgttaccga agcaggattc ggggccgacc taggagctga
     1021 aaaattccta aatatcaaag tacctaatct tcctaaagca ccagatgcta tcgtcattgt
     1081 tgcaaccttg cgcgccctta aaatgcatgg aggtgtcgcc aaatcagatt tagccgctga
     1141 aaattgtgag gctgttcgct taggatttgc caacttaaaa cgtcacgtgg aaaacatgcg
     1201 ccaattcaaa gttccagttg tggtggctat caatgaattt gtagcagata ccgaggctga
     1261 aattgctacc ttaaaagcgc tttgtgaaga gattaaggtt ccagtagaat tagccagtgt
     1321 ttgggcaaat ggagctgagg gaggcttagc attagctaaa acagtggttc gtgtgattga
     1381 tcaagaagct gctgactaca aacgtctcta ttcagatgag gatactttgg aagaaaaagt
     1441 gatcaatatc gttacccaga tttatggtgg caaagctgtt caatttggcc ccaaggccaa
     1501 aacacaattg aagcagttca cagaattcgg ttgggataaa ttgcctgttt gtatggctaa
     1561 gacccaatac agtttttcag ataacccatc cttgcttgga gcgccaaccg attttgatat
     1621 taccattcgt gaatttgttc ctaaaacagg ggcaggtttt attgttggtt taacaggtga
     1681 tgtgatgacc atgcctggtt tgcctaaagt tccagctgcc atggcaatgg acgtagcaga
     1741 aaatggcaca gccctcggtc tcttttagga aaaattgctc gaataattcg agcaagcgct
     1801 cctttcttcc cgctttactg tgatataata aggtaactga gattttggag ggatgattat
     1861 caaaaagaaa gcaaaagtaa aatatttgct ccataaaggg aaacatggat ttttaagagg
     1921 aatttttagt agaacaacaa tcattgtatt attaattatt ttgcaattag ttttcttatt
     1981 tcaatcttat gcctggatgg agcagtaccg tgtttggata acgattcttg aaagtgtctt
     2041 tgctattact attgttttat atttagttaa tagtgatatg gatgctattt caagaatgac
     2101 gtggttaatt ttgattatga ttgctccatt actagggtca ctgtttttaa tttatacaaa
     2161 attggattgg ggttacagag ggctaaaaca aagaataaat catcttgtag atctgtctgc
     2221 accttacctt agcgatgatg acgctatatt agaggtatta aaggatagca catcaaccac
     2281 atatcattta gtacagtact tagaaagaag tcgtggcaac tttccgattt ataataatac
     2341 aagggtaact tacttcccaa caggtgaaac tttttttgat agtttaaaag aacagttatt
     2401 cttagctaaa aagtacattt ttcttgaatt ttttattatt gcagaaggcc aaatgtgggg
     2461 agaaatcctt agtattctag aaaaaaaagt cagtgaaggt gtagaagtta gggtgttgtt
     2521 tgatggcatg aacgaactat caacgctatc atcagattac gccaagagat tagaacaaat
     2581 agggattaaa gctaaatcat
//
LOCUS       DAUVUY010000140         2507 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.141, whole genome shotgun sequence.
ACCESSION   DAUVUY010000140 DAUVUY010000000
VERSION     DAUVUY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.141"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(42..596)
                     /locus_tag="VOX58_001887"
     CDS             complement(42..596)
                     /locus_tag="VOX58_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HES5879087.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(743..1525)
                     /gene="codY"
                     /locus_tag="VOX58_001888"
     CDS             complement(743..1525)
                     /gene="codY"
                     /locus_tag="VOX58_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HES5879088.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASIIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(1743..>2507)
                     /locus_tag="VOX58_001889"
     CDS             complement(1743..>2507)
                     /locus_tag="VOX58_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HES5879089.1"
                     /translation="EAGWYPDIADIKSKITSRTKAIVVINPNNPTGALYPKEILEDIV
                     ALAREHQLIIFADEIYDRLVMDGKEHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWM
                     VLSGPKDHVKGYIEGLNMLANMRLCSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQR
                     NFIYEAINAIPGLSAVKPEAGLYLFPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRG
                     FNWKDPDHFRIVYLPSVEELEDVQEKITRVLYQYRR"
BASE COUNT          799 a          521 c          401 g          786 t
ORIGIN      
        1 ccaaactctt agtcactgaa tcaatagttt ggtaggctct tttagtaatc gttaataagc
       61 ttagcaccaa gaacctgctc aaagtgagcc aaagaccact cataagattc ttttgtaagg
      121 ctagcgacag ccgatttaac aatctctaat tgataaccaa gatgataagc gtcaatagca
      181 gtatgtaaga cacagatatc agataaaact cctgttaaca ctaattgagt gatacctctt
      241 tctcgtagtc taatatctaa atcagtcccc gaaaaagctg aataataacg cttatcaatc
      301 caaaaaactc tgggatgttg cttcatataa ctgtaaactt cagctagtgg accataaaga
      361 tggcgtccag tagttccttt gatattatgt gctgcaaata acttactctc aggatgccaa
      421 ggatcatttt gatcatgaca atcaatagca aaaaaaatat agtctccttg atcaaatgcc
      481 gttttagtta cctctgctat tttagtggca atagcctgag cagatttacc tgcacttaat
      541 ttaccatcat ctgctacaaa atcatttgtg taatcaattg aaatcaatgc tctcatcact
      601 ctctcctaaa cagggaaacc tctcccctta tctcttatat atggcattgt atcagttttt
      661 attacaggta tcaagacatc tatttgcatc taccattaac agcaaaaaac atctcttttc
      721 aagagatgtt gtctttttct atttaaaatt cttttaattt agcaaaaata ccttcgttga
      781 taactttgag gtatgtccct ttcataccaa gagaacgact ttcaataatc cctgcacttt
      841 ctaatttacg cagagcattg acaataacag aacgggtaat accaatacga tcagcaataa
      901 tagaagctgt caatcgtcct tcgttaccat ctaactctcc aagaattgct gcaacagctt
      961 tcatttctga ataagaaagc gtattaattg ccatgttgac cgctgtttgt ttacggatgg
     1021 tgtcttctaa gttttctgtc tgaagattta atagttgaat ccccacaaca gtacttgaga
     1081 tctcaaccaa aatcaaatca tcatcactaa actcattgtc attacgccag ataatgagtg
     1141 atccaaggcg catccctcca ccataaatcg gcgcaatagt cgttagacct cctggataag
     1201 tgtctttaga ctcaacaggg aatatagtca attcattttc gacagaaagg ttagcttctg
     1261 tatcgtaaac tcggctagca gcctttacat aagtatctgg aaattgttta gcttcaaaaa
     1321 attcctcaac acgatcagtg ttagttttat atttcatggc ataaccaagc agtgtgccgc
     1381 cgccattgat aatacaagca ttgcagtcaa tgatatctgc taggcgagat gccattgtgt
     1441 tatacggtaa ttctgtttct aggctatcta cggaacgctg caaaatagat gtgattttac
     1501 gagttttttc taataagtta ggcatttttt tccttttcct ttgaattatt tgcgctttca
     1561 ttataacaaa gctaactcgc ttttgcaagg aattatcaga aaattttcta ttcttactac
     1621 aattccaact attgatgcct tattatcact tcatttaaag gagataagca ctagcttgtt
     1681 agagcatagg atcacattct tttaagaaac gctattttta ttaatcaaag atgagcattg
     1741 cattaacgtc tatactggta taagacacgt gtaatctttt cttggacatc ttctaattct
     1801 tctacacttg gtaggtaaac aatacggaag tgatctggat ctttccagtt aaaaccacgt
     1861 ccatgtacca acatcacttt ttcttgcttt aataactgta agacaaattc ttcatcatca
     1921 tcaatacgat acatctgacg atcaattttc ggaaataaat aaagacctgc ttcaggttta
     1981 actgccgata agccagggat agcattaata gcttcataaa taaaattacg ttgctcaaaa
     2041 atacgccctc ctggcagtaa cagtgcatca actgattgat gacctcctaa ggaagtttga
     2101 acaacttgtt gggctaaaac attggagcat aaccgcatat tggcaagcat gtttaaccct
     2161 tcaatgtaac ctttaacatg gtctttagga cctgataaga ccatccatcc aacacgaaaa
     2221 ccagcaatac gatgagattt ggaaagacca ttcatagata cacaaaaaac atcaggtgcc
     2281 aaacttgcaa tagcaatatg ctctttacca tccatcacca aacgatcata gatttcatcg
     2341 gcaaaaataa tcagctggtg ttctcttgcc aaagcaacga tgtcttctaa aatctcctta
     2401 ggataaaggg ctcctgttgg gttattagga ttgatgacca caatagcttt ggtgcgactc
     2461 gtgatttttg atttgatatc agcaatatca ggataccaac cagcttc
//
LOCUS       DAUVUY010000141         2505 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.142, whole genome shotgun sequence.
ACCESSION   DAUVUY010000141 DAUVUY010000000
VERSION     DAUVUY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.142"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     repeat_region   218..517
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=284..319
                     /rpt_unit_seq="gttttgggaccattcaaaacagcatagctctaaaac"
     gene            complement(620..1282)
                     /gene="csn2"
                     /locus_tag="VOX58_001890"
     CDS             complement(620..1282)
                     /gene="csn2"
                     /locus_tag="VOX58_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HES5879090.1"
                     /translation="MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYKYEEDSEL
                     KFFDRKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTK
                     KKLLIFVNSGAFLTKDEVASLQEYISLTNLTVLFLEPRELYDFPQYILDEDYFLITKN
                     MV"
     gene            complement(1272..1613)
                     /gene="cas2"
                     /locus_tag="VOX58_001891"
     CDS             complement(1272..1613)
                     /gene="cas2"
                     /locus_tag="VOX58_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HES5879091.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
     gene            complement(1610..2479)
                     /gene="cas1"
                     /locus_tag="VOX58_001892"
     CDS             complement(1610..2479)
                     /gene="cas1"
                     /locus_tag="VOX58_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HES5879092.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVK
                     SQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFSYNGKEMYLTNIISDYTKKVVKA
                     LNNEGKGVPEFRI"
BASE COUNT          839 a          493 c          361 g          812 t
ORIGIN      
        1 aattttccaa gattgacgac tatccaattc aactgcacaa tcatcaatag aaataataaa
       61 atcaatcatc gtagtcacct catctatagt gtactacttt gattgatttt gtcattatga
      121 atctagtttt ctgaaaacaa aagtttcttc atgcgtttgg cgtgggtatc cgactgctgg
      181 tattaaccct ctttctcaag ttatcatcgg caatgttgaa tggagaccat tcaaaacagc
      241 atagctctaa aacggaaagc aggagaaagg acgttaatat gtcgttttgg gaccattcaa
      301 aacagcatag ctctaaaacg tcaaactcaa ttttttgtct agctctattg ttttgggacc
      361 attcaaaaca gcatagctct aaaacagaca aaagaaaaag cattgtcaat ttctagtttt
      421 gggaccattc aaaacagcat agctctaaaa caataatcag tttaaaaatc catctggagc
      481 agttttggga ccattcaaaa cagcatagct ctaaaacctc gtagactatt tttgtctaaa
      541 aaatttcgta atcgcactat ttgtctcagc tagacttcag tcttgaaaag cccctgtatt
      601 actgcattta ttaagagtat tataccatat ttttagttat taagaaataa tcttcatcta
      661 aaatatactg cggaaaatca tatagttcac gtggttctaa aaagagaact gttaaatttg
      721 tcaatgatat atactcttgt aaactagcca cttcatcctt tgttagaaaa gctccgctat
      781 tgacaaaaat aagcaacttt ttcttagtga gatatttgaa aatttgaagt atctctagac
      841 atttttcaaa aatagtatca ctttgcgttt ctacttttac tcctaaggac ttaatcaatt
      901 ccaggattgt gatttcatca tactctaaat ctaattcatt ttctaagcat tcaaagacaa
      961 tcagttctgt aatcgtagca accaatttgt caatcatcga tttcacttcg ggtttctcat
     1021 taaattgaga ttctaaatct gcatgaatca attttaaaat ggttgaggag ttaacatcaa
     1081 atcctaaaat atctgttaca agcatgattt ctgattcttt gatggttttc atcttgcgat
     1141 caaaaaattt aagttcagaa tcttcctcat atttgtaaca atattgcact atttttgaaa
     1201 atacacagac atcttcgagc acaagaattg taccgcctct taatggaatc ggttcatcta
     1261 gtaaggaaaa attaagattc atcaaaagcc tccccaagaa atacaagtct ttcatcggag
     1321 tttgcaatac aattatttct ttcaccatgt aaataaatca ttcgtgcaaa ctgtttttct
     1381 gtgaccgtta gtaatgtaat atttccttta ttaggattat gctccctcag ccgaccaatc
     1441 atggcattgt tagctgtatt attcaacagc aacttactat aaatagaaaa ttgatgcatg
     1501 ataaaccctt cactaagtaa aaatttccga aattttcgat aggcttttcg ttcctcagcg
     1561 gtgtccgtcg gcatatcaaa cataagtatc attctcatat atctataact catatcctaa
     1621 attcaggaac tcctttccct tcattattca gagctttgac aactttttta gtataatcgc
     1681 taataatatt cgtgagatac atctctttac cattatatga aaatgtatct gaaaacaaag
     1741 taaataactc tctctttatt ttgggaaaag gctgatttcg attttcataa acaatcttat
     1801 ccaccaaagg cctaaatggt tccataatat cgctagcaaa attgaactga ttaaactgat
     1861 tagcgtgttt aagcccaaac tgagtcatac atccagacac aaccacttca cgcgcaaaca
     1921 tactcaataa taaagtataa ccataatcca gacctgcatt gattggatgc tccaaatctc
     1981 ttgagaaatc gttcccaaaa agtgtattaa aataaattct cgctgcatgc ccttctcgat
     2041 tactcggatc aaaattttcc aaaccatgat ataaatccat aatagattgg gatttttcaa
     2101 aataggagca tgctcctaga tagcaagatt gattcaaaat cttttgagca ataatcgtcg
     2161 tccaaacctg cgatttgact gtttctgacc aggacatttg tttcccaagc tgtaaactcg
     2221 aatcatgacg accataaaaa ggcatcagca tagctgttgg taatcgttta tcatcacaga
     2281 atatgacaag gacattctca tccactagcc gttttaccag catagtggac aagacaatat
     2341 cggtcgtttc taataacaaa atatcaattt ctgataaatg gatcagctcc gttttatagg
     2401 catccttaaa aatcagatga ttattcttat aggataattt cgagtgggta tttaccacaa
     2461 cagtacgcca accagccatc agtcacctcc tagctgactc aaatc
//
LOCUS       DAUVUY010000142         2449 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.143, whole genome shotgun sequence.
ACCESSION   DAUVUY010000142 DAUVUY010000000
VERSION     DAUVUY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.143"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..143
                     /locus_tag="VOX58_001893"
     CDS             <1..143
                     /locus_tag="VOX58_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hyaluronan synthase"
                     /protein_id="HES5879093.1"
                     /translation="KHPASFLLSPLYGILHLFVLQPLKLYSLCTIKNTEWGTRKKVTI
                     FK"
     gene            179..1387
                     /gene="hasB"
                     /locus_tag="VOX58_001894"
     CDS             179..1387
                     /gene="hasB"
                     /locus_tag="VOX58_001894"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HES5879094.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEDYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFDNTYLALRVAYF
                     NELDTYAESRKLNSHMIIQGISHDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEERESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILKSKDIKIIIYEPMLNKLESEDQSVLVNDLENFKKQANIIVTNRYDNELQDVKNK
                     VYSRDIFNRD"
     gene            1569..>2449
                     /gene="hasC"
                     /locus_tag="VOX58_001895"
     CDS             1569..>2449
                     /gene="hasC"
                     /locus_tag="VOX58_001895"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HES5879095.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     DILERQTPGAGNEVQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALEH
                     PEVKEDLKNYIIKLGKA"
BASE COUNT          863 a          426 c          441 g          719 t
ORIGIN      
        1 tcaaacatcc tgctagtttt ttgttatctc ctctgtatgg aatattacac ttgtttgtct
       61 tacagcccct aaaactttat tctttatgca ccattaaaaa tacggaatgg ggaacacgta
      121 aaaaggtcac tatttttaaa taatatatgc atcgagtagt tagagaagga gtaattttat
      181 gaaaatagca gttgctggat caggatatgt tggattatca ctaggagttc ttttatcact
      241 tcaaaacgaa gtcactattg ttgatattct tccttctaaa gttgataaga ttaataatgg
      301 cttatcacca attcaagatg aatatattga agattactta aaaagtaagc aattatctat
      361 taaagcaact ttagatagca aagcagctta taaagaagcg gaactggtca ttattgccac
      421 acctacaaat tacaacagta gaattaatta ttttgataca cagcatgttg aaacagttat
      481 caaagaggta ctaagcgtta atagccatgc aactcttatc atcaaatcaa caattccaat
      541 aggtttcatt actgaaatga gacagaaatt ccaaactgat cgtattatct tcagccctga
      601 atttttaaga gagtctaaag ctttatatga caacttatat ccaagccgaa ttattgtttc
      661 ttgtgaagaa aacgattctc caaaagtaaa ggcagacgca gaaaaatttg cacttttatt
      721 aaagtctgca gctaaaaaaa ataatgtacc agtacttatt atgggagctt cagaagctga
      781 agcagtaaaa ctatttgaca atacttattt agcgttaagg gtagcttatt ttaatgagtt
      841 agacacttac gcagaatcga gaaaattaaa tagtcacatg attattcaag gaatttctca
      901 tgatgatcga ataggaatgc attataataa cccatcattt ggttatggag gttattgtct
      961 acctaaagat acgaagcaat tattggcaaa ttacaataat attccccaaa cgctaattga
     1021 agctatcgtt tcatcaaata atgtgcgcaa gtcctatatt gctaagcaaa ttatcaacgt
     1081 cttagaagag cgggagtccc cagtaaaagt agtcggggtt taccgtttaa ttatgaaaag
     1141 taactcagat aattttagag aaagtgctat caaagatgtt attgacattc ttaaaagtaa
     1201 agacattaag ataattattt atgagccaat gttaaacaaa cttgaatctg aagatcaatc
     1261 tgtacttgta aatgatttag agaatttcaa gaaacaagca aatattatcg taactaatcg
     1321 ctatgataat gaattacaag atgttaaaaa taaagtttac agtagagata tttttaatag
     1381 agactagcca tgtcttcatc tataacaata atattcattt ttactttaaa agaactgcag
     1441 ttggtggtat gattgagcac tatgaaatag tcgaaaaatc atatgaaaac gtaacatgat
     1501 gtcaaatgaa ttccgttctg aaaacgaatg tcactaagcc atagattata aaaagtgagg
     1561 agttactgat gaccaaagtc agaaaagcca ttattcctgc tgcaggtcta ggaacacgtt
     1621 ttttacctgc taccaaagct cttgccaaag agatgttgcc catcgttgat aaaccaacca
     1681 tccagtttat cgtcgaagaa gcgctaaaat ctggcatcga ggaaatcctt gtggtgaccg
     1741 gaaaagctaa acgctctatc gaggaccatt ttgattcaaa ctttgaatta gaatacaacc
     1801 tccaagctaa ggggaaaaat gaactgttga aattagtgga tgaaaccact gccattaacc
     1861 ttcattttat ccgtcaaagc cacccaagag gactgggaga tgctgtctta caagccaaag
     1921 cctttgtggg caatgaaccc tttgtggtca tgcttggaga tgacttaatg gacattacaa
     1981 atgcatccgc taaacctctc accaaacaac tcatggagga ctatgacaag acgcatgcat
     2041 ccactatcgc tgtgatgaaa gttcctcatg aagatgtgtc tagctatggg gttatcgctc
     2101 ctcaaggcaa ggctgtcaag ggcctttaca gtgtagacac ctttgttgaa aaaccacaac
     2161 cagaagatgc gcctagtgat ttggctatta ttggtcgtta cctcctaacc cctgaaattt
     2221 ttgatatttt ggaaagacag acccctggag caggtaacga agtgcaactc acagatgcta
     2281 tcgataccct caataaaact cagcgtgtct ttgcacgaga atttaaaggt aatcgttacg
     2341 atgttgggga taaatttgga ttcatgaaaa catctatcga ctatgcctta gaacacccag
     2401 aggtcaaaga ggacttgaaa aattacatta tcaaactagg aaaagcttt
//
LOCUS       DAUVUY010000143         2440 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.144, whole genome shotgun sequence.
ACCESSION   DAUVUY010000143 DAUVUY010000000
VERSION     DAUVUY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.144"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>2440
                     /gene="mfd"
                     /locus_tag="VOX58_001896"
     CDS             <1..>2440
                     /gene="mfd"
                     /locus_tag="VOX58_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HES5879096.1"
                     /translation="DYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSN
                     LNYFADNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIK
                     RYQKNQTTVIVQVESQYAYKRLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFY
                     FADEKLALITEREIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLG
                     IETIQIQGIHRDYVTIQYQNSDRISLPIDQIGSLSKYVSADGKEPKINKLNDGRFQKT
                     KQKVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKE
                     IKADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENF
                     KARFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIV
                     IDEEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYP
                     VQTYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVSELQELVPEASIGFVHG
                     QMSEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRG
                     RVGRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNIL
                     GASQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYISDER
                     QKIDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEIALLKHYMDNAFAELVE
                     RKNNQVIVRFEVTSL"
BASE COUNT          791 a          399 c          496 g          754 t
ORIGIN      
        1 gattatattc ctaagggaac gccaatcttt tttgatgatt ttcaaaaact agttgataaa
       61 aatgcaagat ttgatttaga gattgctaat ctcttgacag aagatttaca gcaaggaaag
      121 gctctttcca atcttaacta ctttgcagat aattatcgag agcttaggca ctataagcca
      181 gcgaccttct tttcaaattt tcataaggga cttggaaata tcaaatttga tcagatgcat
      241 cagctaactc agtatgccat gcaggaattt tttaatcaat ttcctttgtt gattgatgag
      301 attaaacggt atcaaaaaaa tcaaacaacg gttattgtac aggtagagtc tcagtatgct
      361 tacaaacgac ttgaaaaatc ttttcaagat taccaatttc gccttccttt agtgagtgct
      421 aatcaaattg tttcacgtga atcacaaatt gtaattggag ctatctccag tggtttttat
      481 tttgctgatg aaaagttagc gttaatcaca gagcgtgaaa tttatcataa aaagatcaaa
      541 cgacgcgcta gacgatctaa tattagcaat gctgagcgtt tgaaagatta caatgagtta
      601 gcagtaggtg attacgtggt tcataatgtc catggtatcg gtcgctttct tggaattgaa
      661 acgattcaga ttcagggaat ccatcgagat tatgttacta ttcaatatca aaattcagac
      721 cgtatttctc ttccaattga ccaaattggt agcttatcaa agtacgtttc tgctgatgga
      781 aaagaaccta aaattaataa actcaatgat ggtcgtttcc aaaagacaaa gcaaaaggtc
      841 gctagacaag tagaggatat tgctgatgac cttctaaaat tatatgctga aagaagtcag
      901 caaaaaggat tttcattttc accagatgat gacttgcagc gcgcttttga tgatgatttt
      961 gcttttgtag aaacagaaga tcaacttagg tctataaagg aaattaaagc tgatatggag
     1021 agcatgcagc ctatggatcg tcttttagta ggcgatgtag gatttggtaa gacagaagta
     1081 gctatgaggg cagcctttaa agcggtgaac gatcacaaac aagtagctgt cttagttcca
     1141 accacagtct tggcccagca gcattatgaa aacttcaaag cacgctttga aaattaccct
     1201 gttgaggttg acgtcttaag tcgtttccgt agtaaaaaag agcaagctga aacactagaa
     1261 cgtgtacgaa aaggtcaaat tgacattatt atcggaaccc atcgactcct atcaaaagat
     1321 gtggtctttt ctgatttagg attgattgtg attgatgagg aacaacggtt tggtgttaag
     1381 cataaagaaa ctttaaagga attaaaaact aaggttgacg tcttaacctt aacagctact
     1441 ccgattccta gaaccttaca catgtctatg ttaggtatcc gagatttatc ggttattgag
     1501 accccaccaa ccaatcgtta tcctgttcaa acctatgttt tggaaaataa tccaggtctc
     1561 gttagagaag ctatcattcg tgaaatggat cgtggaggac aaatttttta cgtttacaat
     1621 aaagttgaca ctattgaaaa gaaagtttca gagctacaag aattagtccc agaagcttct
     1681 ataggttttg ttcatgggca aatgagtgaa attcaacttg aaaatacctt gattgacttt
     1741 ataaatggtg attatgatgt ccttgtggct acaacaatca tcgaaacagg agttgacatt
     1801 tctaatgtaa acactttgtt tattgagaat gctgatcata tgggattgtc aactttatat
     1861 caactacgag ggcgcgtcgg aagaagtaat cgtattgctt atgcttacct gatgtatcgt
     1921 cctgataagg tcctaacaga ggtttctgaa aagcgtttag aggctattaa aggctttact
     1981 gaattaggtt caggcttcaa gattgctatg cgagatttgt ctatacgagg agcaggtaat
     2041 attttagggg cttctcagag tggctttatt gattcggtcg gttttgaaat gtattctcag
     2101 ttattggaac aggctattgc tagcaagcaa ggaaaaacga ctgttcgcca aaaaggtaat
     2161 actgaaatca atcttcagat tgacgcctat ttgccagatg attacatatc agatgagcgt
     2221 caaaaaattg acatttacaa gcgtattcga gaaattcaat caagagaaga ttatctcaat
     2281 ttgcaggatg agctgataga tcgttttgga gagtatcctg atcaagttgc ctatttgtta
     2341 gagatagctc tgctaaaaca ttatatggac aatgcctttg cggaattagt tgaacgcaaa
     2401 aataatcagg tcattgttcg atttgaagta acttctttaa
//
LOCUS       DAUVUY010000144         2350 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.145, whole genome shotgun sequence.
ACCESSION   DAUVUY010000144 DAUVUY010000000
VERSION     DAUVUY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.145"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            207..>2350
                     /locus_tag="VOX58_001897"
     rRNA            207..>2350
                     /locus_tag="VOX58_001897"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          714 a          448 c          661 g          527 t
ORIGIN      
        1 cacatggtca gattcctaat tttctacaga agtttcgcta aagcgagcgt tgcttggtat
       61 cctatataat agtccattga aaattgaata tctatatcaa attccacgat ctagaaatag
      121 attgtagaaa agtaacaaga aaataaaccg aaaacgctgt gactatttga taagttttct
      181 agtttttaaa aaaactaggt taataaggtt aagttaataa gggcgcacgg tggatgcctt
      241 ggcactagaa gccgaagaag gacgtgacaa acgacgaaat gctttgggga gctgtaagta
      301 agcgctgatc cagagatgtc cgaatggggg aacccactaa ctaatggtta gtatccataa
      361 ctgttaaggt tatgagaagg aagacgcagt gaactgaaac atctaagtag ctgcaggaag
      421 agaaagcaaa cgcgattgcc ttagtagcgg cgagcgaaac ggcaggaggg caaaccgaag
      481 tgtttacact tcggggttgt aggactgcga cgtgggactt taaaaggata gaagaattac
      541 ctgggaaggt aagccaaaga gagtaaaagc ctcgtattta aaattctttt gagccctagc
      601 agtatcctga gtacggcgag acacgtgaaa tctcgttgga atctgggagg accatctccc
      661 aaccctaaat actctctagt gaccgatagt gaaccagtac cgtgagggaa aggtgaaaag
      721 caccccggga ggggagtgaa atagaacctg aaaccgtgtg cctacaacaa gttcgagccc
      781 gttaatgggt gagagcgtgc cttttgtaga atgaaccggc gagttacgat atgatgcgag
      841 gttaagttga agagacggag ccgtagggaa accgagtctt aatagggcga tttagtatca
      901 tgttgtagac ccgaaaccat gtgacctacc catgagcagg ttgaaggtga ggtaaaactc
      961 actggaggac cgaaccaggg cacgttgaaa agtgcttgga tgacttgtgg gtagcggaga
     1021 aattccaaac gaacttggag atagctggtt ctctccgaaa tagctttagg gctagcgtcg
     1081 atgttaagtc tcttggaggt agagcactgt ttgggtgagg ggtccatctc ggattaccaa
     1141 tctcagataa actccgaatg ccaacgagat atcatcggca gtcagactgc gagtgctaag
     1201 atccgtagtc gaaagggaaa cagcccagac caccagctaa ggtcccaaaa taactgttaa
     1261 gtggaaaagg atgtggggtt gcacagacaa ctaggatgtt agcttagaag cagctattca
     1321 ttcaaagagt gcgtaatagc tcactagtcg agtgaccctg cgccgaaaat gtaccggggc
     1381 taaaacagtt taccgaagct gtggatgaca caaaagtgtc atggtaggag agcgttctat
     1441 gtgtgaagaa ggtgtaccgt gaggagcgct ggaacgcata gaagtgagaa tgccggtatg
     1501 agtagcgaaa gacaggtgag aatcctgtcc accgtaagac taaggtttcc aggggaaggc
     1561 tcgtccgccc tgggttagtc gggacctaag gagagaccga aaggtgtatc cgatggccaa
     1621 caggttgata ttcctgtact agagtatata gtgatggagg gacgcagtag gctaactaaa
     1681 ccggacgatt ggaagagtcc ggctaagcag tgaggtgtaa gatgagtcaa atgcttatct
     1741 ttataacatt gagctgtgat ggggagcgaa ttttagtagc gaagttagtg atgtcacact
     1801 gccaagaaaa gcttctagcg tttaatgata ctctacccgt accgcaaacc gacacaggta
     1861 gtcgaggcga gtagcctcag gtgatcgaga gaactctcgt taaggaactc ggcaaaatga
     1921 ccccgtaact tcgggagaag gggtgctgac ttaggtcagc cgcagtgaat aggcccaagc
     1981 aactgtttat caaaaacaca gctctctgct aaatcgtaag atgatgtata gggggtgacg
     2041 cctgcccggt gctggaaggt taagaggagg gtttagcgca agcgaagatc tgaattgaag
     2101 ccccagtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt
     2161 aagttccgac ccgcacgaaa ggcgtaatga tttgggcact gtctcaacga gagactcggt
     2221 gaaattttag tacctgtgaa gatgcaggtt acccgcgaca ggacggaaag accccatgga
     2281 gctttactgc agtttgatat tgagtatctg taccacatgt acaggatagg taggagccat
     2341 tgacttcggg
//
LOCUS       DAUVUY010000145         2268 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.146, whole genome shotgun sequence.
ACCESSION   DAUVUY010000145 DAUVUY010000000
VERSION     DAUVUY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.146"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..626
                     /locus_tag="VOX58_001898"
     CDS             <1..626
                     /locus_tag="VOX58_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HES5879097.1"
                     /translation="DPKNADLADLGAVMNNVYYRKEDMLACINQFYEDMIDRSETMKQ
                     HPNYKTGENYAYLGLSANFLIFDEYVAMMDMLGRESTTVIHKLKQIVMLGRQAGFFLI
                     LACQRPDAKYFSDGIRDNFNFRVALGRMSEMGFGMMFGSDTQKQFFLKPIKGRGYVDT
                     GKSVISEFYTPLVPKRYDFLGEIGKVIQKKQSEPVPHEVKGTGSDLL"
     gene            808..1992
                     /locus_tag="VOX58_001899"
     CDS             808..1992
                     /locus_tag="VOX58_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HES5879098.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            1989..>2268
                     /locus_tag="VOX58_001900"
     CDS             1989..>2268
                     /locus_tag="VOX58_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879099.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERR"
BASE COUNT          760 a          388 c          471 g          649 t
ORIGIN      
        1 ttgaccctaa aaatgctgat ttggcagact taggggcggt catgaataat gtctattacc
       61 gtaaagaaga tatgttggca tgtatcaatc aattttacga agatatgatt gatcgcagtg
      121 aaaccatgaa acaacaccct aactataaaa caggagaaaa ttatgcttat cttggtttgt
      181 ctgccaattt tcttattttt gatgaatatg tggcaatgat ggatatgcta ggtcgtgaaa
      241 gtaccaccgt tattcataaa ctcaaacaaa ttgtaatgtt aggacgacaa gcaggcttct
      301 ttttaattct tgcctgtcaa cgtccagacg caaaatattt tagtgatggg attcgggaca
      361 actttaactt ccgtgtggct ttgggtcgta tgtctgaaat gggctttggt atgatgttcg
      421 gaagcgatac acaaaaacaa tttttcttaa aacccatcaa gggaagaggt tacgtggata
      481 caggaaaaag tgtgattagt gagttttata caccccttgt accaaaaagg tacgatttct
      541 taggagaaat aggaaaagtc attcaaaaaa agcaatctga gcctgtaccg cacgaagtga
      601 aaggtacagg ttcagacttg ctttgattgg tgtggcgtta gccacgccaa tcctttagcc
      661 ccctctatct aacagggggg cacaaataca actgaaaaca ggcaattctc atcaaccaat
      721 gttgatggta caaaggattg cggaaaatat gaaaggtgta aactcaaggg tcatagttta
      781 catctttttt agattggagg tttgcgaatg gaaaacgaaa aaatttggat taaggaattg
      841 aaagaaaagc gactggctta tggcgtttct caaaataaac tggcggttgc ttctcatatt
      901 accagacctt acctatcgga tattgaaaca ggcaaggcag tccctactca acaggtgaaa
      961 gaagatttac tgaacgcttt ggaacgcttc aatcccgata acccattgga gatgttattc
     1021 gactatgtac ggattcgatt ccctaccaat gatgtggctc aaattatcgg tgaggtttta
     1081 cgtttgaata tggattatat gctacacgaa gactttggct attattctta tcccgaacac
     1141 tatcgtttcg gtgatattgt tgtcctagtc tctcatgatg taagtaaagg tgttttatta
     1201 gaactgaaag gaaaaggctg tcggcaattt gaaaactttc tattggctca acatcggagc
     1261 tggtatgatt tctttcaaga ttgcatggaa cataaaggta ttttcaaacg cttggattta
     1321 gctatcaacg ataaaacagg gattttgaat attccagatt tggcaaagaa atgtaaacag
     1381 gaagaatgca tttccgtctt tcgaagcttt aaaaattatc gcagtggtga attggttcac
     1441 cgtgatgaaa aaccatacat gggaaacaca ctttatattg gctcattaaa aagtgaagtc
     1501 tatttctgta tatatgagaa agattatgag caatacgtga aaaatgatgt tcccttagaa
     1561 gacgcagaag taaagaatcg gtttgaaatt cggttaaaaa atgaccgagc cacccaagcc
     1621 atgaaagatt tattggcaca tcaacaggcg gaaaaaacag cctttgaaat tatcaatcgc
     1681 tatatccgct ttgcagataa agacgacact aaacgtcgga gtgattggaa aactaatgaa
     1741 cgttgggaat ggtttatcgg taaaaatcgt ggagcattac gcctaaccac acagccagag
     1801 ccttattctt ttgaacgaac attgaactgg ttacatcatc aagtcgcccc tacattaaaa
     1861 atcgcaagca ttttagatgt tctcaacggt acaaccatta tctccaccat gattcaggaa
     1921 gccaaactga cagaaaaaca tgagaaatta attgaacaac aacatctagc catggaggat
     1981 ttgattacat gagcctttta acaaaagaat taaagaagtt agggtttcag tgtggcattg
     2041 agtttcaagc gtatatacaa aatacaggga aatatacgtc tctcattatt gagggaaaac
     2101 ggcaagctgg tgataccatc tatacctatg atttttacaa ggtgagattt tataataatt
     2161 ataccaatcg agtaaccgtc tatggagagc atttaacccc ttttcaatta ttaaggcgtg
     2221 taaaaagcta tatctattat cgagaaaaat atcttaagga aaggagga
//
LOCUS       DAUVUY010000146         2261 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.147, whole genome shotgun sequence.
ACCESSION   DAUVUY010000146 DAUVUY010000000
VERSION     DAUVUY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.147"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1145
                     /locus_tag="VOX58_001901"
     CDS             <1..1145
                     /locus_tag="VOX58_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HES5879100.1"
                     /translation="QSNSMVSSAIIEDINGIETIKSLTSEENRYQKIDSEFVDYLDKS
                     FKLSKYSILQSSLKQGAQLILNVIVLWFGAKLVMGGKISVGQLITFNTLLSYFTNPLE
                     NIINLQTKLQSAKVANNRLNEVYLVDSEFQEAGTLVNQELLHGDIQFEELSYKYGFGR
                     DTLSNINLTIKQGDKVSLVGISGSGKTTLAKMIVNFFEPYNGRITINHNDLKMIDKKS
                     LRQHINYLPQQAYIFNGSILENLTLGANDCTSHEDILRACEVAEIRQDIEQMPMGYQT
                     ELSDGAGLSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKRVIDNLLAMTDKTI
                     IFVAHRLSISARTNQVIVLDEGKIIEIGSHQELMTKQGFYHHLFSS"
     gene            1156..>2261
                     /locus_tag="VOX58_001902"
     CDS             1156..>2261
                     /locus_tag="VOX58_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HES5879101.1"
                     /translation="MNPNLFKSAEFYHRRHHNFATFLILPSVLLVSFLVLFSLFAKKE
                     ITITSQGELTPTKVIASVQSTSDHTIVVNNLKNNKFIKKGDVIIQYSKTMENSQKKAL
                     EKRLATLNKQKNGLQILRTSIEQETNLFKGEDEFGYSDTFNHFLKQTQDIIIGIAKKN
                     AEINNQASLANHAISVITSQLDELHQKITDYEELKQAINNQEYKLSPENPHQTALKKY
                     QTQFQAQEDNSALKEEYLSQIPQNISELESSMANLSIQRASTGNFSLPDTSHRIKIDI
                     LKTQFLQNASQQLTTVENQITELKNQIEQANIQLKNNIIIAPETGTIHLNSEFEGKSL
                     IPNGSEVAQIYPDIQKIKEVFITYYVTSEHVSLL"
BASE COUNT          819 a          409 c          361 g          672 t
ORIGIN      
        1 tgcagagtaa ctctatggtt agctcagcca ttatcgaaga tatcaatggc attgaaacta
       61 ttaaatcact gaccagtgaa gaaaatcgtt atcagaaaat tgatagtgaa tttgtggact
      121 atctggataa atccttcaag ttaagtaagt actctatttt acaaagcagt ctcaaacaag
      181 gggcgcaact aatccttaac gtgatagtct tatggtttgg ggccaaatta gttatgggag
      241 gaaaaatttc tgttggtcag ttgattacgt ttaatactct cttatcttat tttaccaatc
      301 ctttagaaaa tatcattaat cttcaaacta aactgcaatc agccaaggta gctaataatc
      361 gtctcaatga agtttaccta gtcgattctg agtttcaaga ggctgggaca ttagtcaatc
      421 aagaattatt acacggagac attcaatttg aagagttatc ctataaatat ggctttggac
      481 gcgatactct atcaaatatt aatctcacta ttaagcaagg ggacaaagtt agccttgttg
      541 gtattagtgg atctggtaaa acgactctag ctaaaatgat tgttaatttt tttgagcctt
      601 ataacggccg aattaccatt aatcataatg acttaaaaat gattgataaa aaaagtcttc
      661 gccaacatat taattatctc ccccaacaag cctatatttt taatggttct attttagaaa
      721 atttaacctt aggtgctaat gactgtacta gtcatgaaga tattctaaga gcttgtgagg
      781 ttgcagaaat tcgacaagat attgaacaaa tgcctatggg ctatcaaaca gagctttctg
      841 atggtgccgg actctcagga ggccaaaagc aacgaattgc acttgccaga gctctcctaa
      901 ccaaggcgcc tgtgcttatt ctagatgagg ctacaagcgg tcttgacgta ctaactgaaa
      961 agagggttat tgataatctt ttggctatga cggataaaac tattattttc gtagctcatc
     1021 gtctcagcat ttcagcgcgg accaatcaag tcattgtgct tgatgagggt aaaattattg
     1081 aaataggatc tcatcaagaa ttaatgacta aacaaggttt ctatcatcat ttatttagta
     1141 gttaaggaga ataacatgaa tccaaacctt tttaaaagtg cggaatttta tcatcgacgt
     1201 catcataact ttgcaacttt cttgattttg ccatccgttt tgttagtgag cttcttagtt
     1261 cttttttctc tctttgctaa aaaagaaatt accattactt cacaaggaga attgacgcca
     1321 acaaaagtta ttgcttcagt ccaatcaact agtgaccata ctattgtcgt caacaattta
     1381 aaaaacaata aatttattaa gaaaggtgat gttattatcc aatactcaaa aacaatggaa
     1441 aattctcaaa aaaaagcttt agaaaaacga ttagccactc ttaacaaaca aaaaaatgga
     1501 ctccaaatac tcagaaccag tatagaacaa gaaactaact tatttaaggg agaagatgaa
     1561 ttcggctata gcgatacgtt taatcatttt ctcaaacaaa cccaagatat aatcattggg
     1621 attgctaaaa aaaatgctga aatcaataat caagcaagtc ttgctaatca tgccatttca
     1681 gttattacta gtcagcttga cgaacttcat caaaaaatta cagattatga agagctaaag
     1741 caagctatta ataatcaaga atataagctt tcacctgaga atccacatca aactgcccta
     1801 aaaaaatatc aaactcaatt tcaagcacaa gaagataact cagctttaaa agaagaatac
     1861 ctttctcaaa tcccacaaaa tatctcagaa cttgaatctt caatggcaaa ccttagtatc
     1921 caacgtgcta gtacaggaaa tttttctctt ccagacacta gtcataggat caaaatagat
     1981 attttaaaaa cacaatttct gcaaaatgct tcccaacaac ttaccacagt tgaaaatcaa
     2041 attactgaac ttaaaaatca aattgagcaa gccaatattc agctaaagaa caatataatt
     2101 atagctcctg aaacaggaac gatacatcta aacagtgaat ttgaagggaa atctctcatt
     2161 cctaatggaa gtgaggtggc tcaaatctac ccagatatcc aaaaaataaa ggaagtattc
     2221 attacctact acgtcacttc agagcatgtc tcattgttaa a
//
LOCUS       DAUVUY010000147         2229 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.148, whole genome shotgun sequence.
ACCESSION   DAUVUY010000147 DAUVUY010000000
VERSION     DAUVUY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.148"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..824
                     /locus_tag="VOX58_001903"
     CDS             <1..824
                     /locus_tag="VOX58_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HES5879102.1"
                     /translation="VKPTSFLASIGVKKVYNFSELQQAVSQMLNVKFPVYIASGVYEL
                     GELYNLEPRVLVEEFIDGEEYSLESVVRNGIYTPLGITKKIVDEKLFMDEIGHIFPSN
                     LNKEEKSRVYSWAEKLHQILQLNHITTHTEFRIGRNGDIILIEIGARIGGDCIPNLMK
                     NSFIVKGFDFYNTYLKARLGLQEELNTDTNKFSGIVFFGLNPENYGKIFNGVSYDAPK
                     TSLIFEEKIYKKEGTVLPNPINWSNERVGYVCLENKEYAVLEKEMKEILKSLSIE"
     gene            749..1768
                     /locus_tag="VOX58_001904"
     CDS             749..1768
                     /locus_tag="VOX58_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HES5879103.1"
                     /translation="MFGKQGIRCARKGDERNIKIIVNRVNSERNIKSIGICSPSYPGT
                     FHYQKRYKRGVEFLQNKGFTIVEGNLTGKEGYRGEISGGVQDRAEELNQLIPRVDVIM
                     TSIGGYNSNSVLKYIDYDLFKQKSPIFIGYSDTTALALALYKKTGCITYLSQSVISNF
                     GEFEPFNELNYFYFDFMLQSKCETLMVQIPDVWTDEWINWETYERTKKTNKNEWIIFN
                     KGEFNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDLGRLYRNFTTLALHGI
                     FDKIGGLIISKFETIGENSDVINDIINEFVGHRKIPILLNFDCGHTHPSCLMPIGGKI
                     TLSLS"
     gene            1781..>2229
                     /locus_tag="VOX58_001905"
     CDS             1781..>2229
                     /locus_tag="VOX58_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HES5879104.1"
                     /translation="MNNRESKLTGFYDVFEEEVEKINHIHNVFVEESEKFGFQQMKTA
                     MVEHRKKYLDATDVHYTKIFEVHRVKEESVYALQSDLALSMSRCIANLPQQIPVKWIQ
                     EGVLFRDRIPTYKGYRRQFNQILLGAWGIESAYVDAEIIVATWRGLQR"
BASE COUNT          840 a          265 c          447 g          677 t
ORIGIN      
        1 tagtgaaacc aacctctttt ttagcaagta taggagtgaa aaaagtttat aatttttcag
       61 aattacaaca agcagtttct caaatgctta atgtaaaatt tccggtttac atagcttcag
      121 gggtatatga attgggggaa ttatataatt tagaacctag agttttggtg gaagagttta
      181 ttgatggaga agaatatagc ttagagagcg tggtaagaaa tggaatctat acacctctag
      241 ggataactaa aaaaatagtg gatgaaaaat tatttatgga tgaaataggc catattttcc
      301 cttcaaattt aaacaaagaa gaaaaaagta gagtatattc ttgggcagaa aaattacatc
      361 aaattttgca attaaatcat attacgactc atacagaatt tagaattgga agaaatggag
      421 atattatttt aatagaaatt ggagcaagaa tcggtggaga ttgtattcca aacttaatga
      481 aaaacagctt tatagtcaag ggatttgatt tttataatac ttatttaaaa gctagactag
      541 gtttacaaga agagttaaat acagacacta ataagttttc aggaattgta tttttcggac
      601 tcaatccaga gaattacgga aaaatattta atggagtgag ttacgatgca cctaaaactt
      661 cactaatatt tgaagaaaag atatataaaa aggaaggaac tgtattgcca aatccgatta
      721 attggagtaa tgagagagta ggatatgtct gtttggaaaa caaggaatac gctgtgctag
      781 aaaaggagat gaaagaaata ttaaaatcat tgtcaataga gtaaatagtg aaagaaacat
      841 caaatctata ggtatttgtt caccctccta tccggggaca tttcattatc agaaacgata
      901 taagagagga gtagaatttt tacaaaataa aggatttact atagtagagg gaaatttaac
      961 aggaaaagaa ggttatagag gggaaatttc aggaggagtt caagacagag ctgaagaatt
     1021 aaatcaactt attccgcgtg tggatgtcat catgacttcc ataggtggct acaattctaa
     1081 ttctgtatta aagtatatag actacgatct atttaaacag aaatctccta tctttattgg
     1141 atattctgac acaacagctc ttgctttagc cttatataaa aagactggat gtataactta
     1201 cctgtctcag agcgttatct ctaattttgg agagtttgaa ccgtttaatg aattaaatta
     1261 tttctatttt gacttcatgc ttcagagtaa gtgcgaaacc ttaatggtac aaataccaga
     1321 tgtttggacg gatgagtgga taaattggga aacctatgaa agaaccaaaa aaacaaataa
     1381 aaatgagtgg attattttta acaaaggtga atttaatggt actttgatcg gaggaaatct
     1441 cgataccatt gtaggaataa taggaactga gtatatgcca aaaataacag aggataccat
     1501 actcttgtta gaagatgtat atacggatct aggaagacta tataggaatt tcacaacgct
     1561 tgccttacat ggtatttttg ataaaatagg aggacttatt atttcaaaat ttgaaacaat
     1621 aggggaaaat agcgatgtta ttaatgatat cattaatgaa tttgtagggc atagaaagat
     1681 tcctatttta ttgaattttg attgtggtca tactcatcct agctgtttaa tgcctattgg
     1741 tggtaaaatt acgttgagtt taagctaaaa gaggtggtat atgaacaata gagaaagtaa
     1801 attaacgggt ttctatgatg tttttgagga agaagtagaa aaaataaatc atattcataa
     1861 tgtttttgta gaggagtcag aaaaatttgg ctttcaacag atgaagactg ctatggtaga
     1921 gcatagaaaa aaatacttag atgcaaccga tgttcattat acaaaaatat ttgaagttca
     1981 tagagtaaaa gaagagtcag tatacgccct ccaatcagat ttggcactta gtatgtcgcg
     2041 ttgtatagct aacttaccac aacaaatacc tgtaaagtgg attcaagagg gagtgttatt
     2101 tagagataga attcctacat ataaaggtta tagaagacaa tttaatcaaa tattattagg
     2161 tgcatgggga atagaatcag cttatgtgga tgcagaaata atagttgcaa cttggcgagg
     2221 cttgcagcg
//
LOCUS       DAUVUY010000148         2227 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.149, whole genome shotgun sequence.
ACCESSION   DAUVUY010000148 DAUVUY010000000
VERSION     DAUVUY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.149"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..305)
                     /locus_tag="VOX58_001906"
     CDS             complement(<1..305)
                     /locus_tag="VOX58_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HES5879105.1"
                     /translation="MTTMTIAKGSQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQ"
     gene            complement(495..1088)
                     /locus_tag="VOX58_001907"
     CDS             complement(495..1088)
                     /locus_tag="VOX58_001907"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HES5879106.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPILMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNSGDIAALIKQVQSALLSLANPR"
     gene            complement(1085..1912)
                     /gene="mutM"
                     /locus_tag="VOX58_001908"
     CDS             complement(1085..1912)
                     /gene="mutM"
                     /locus_tag="VOX58_001908"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HES5879107.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPGTASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(2084..>2227)
                     /locus_tag="VOX58_001909"
     CDS             complement(2084..>2227)
                     /locus_tag="VOX58_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HES5879108.1"
                     /translation="SGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLGGKGDEDWSE
                     ADL"
BASE COUNT          619 a          538 c          391 g          679 t
ORIGIN      
        1 ttttgaccaa aaacatccca cattcttaca ggaaaggcct gaggctggta atcacttacc
       61 ccgattgtgg ctaaacgcgc agccaattta tcagtcacct ctcctgcttt agtaagatta
      121 gacaggtctc ctttgatatc tgccaaaaag actggcacac cagctaaact taattgctct
      181 gctagcactt ttaaggtcac agttttacct gttccagtcg ctccagcaat caaaccgtga
      241 cgatttagca tccctaattg taaggctatc ggttgactgc cttttgcaat tgtcattgta
      301 gtcataggct tcctcttttt tgatgatcat acttgtcgat atgtgtttac ccataagtta
      361 aagtgtttag aagggtctca ctcttaatac tattataaca tataaccctt gatgcgctaa
      421 tttaaaaggg accagctagt tttagacaga aatagcttga ttttgacagc tatgatacga
      481 atctctcagc ttagctacct cggattggct aatgacaaca acgcagattg tacttgcttt
      541 attaaggctg caatatcccc actattatca atcactagac tggcataggg ctttttttcc
      601 tctattggta actgactggc tattcgctgc cttgcttttc ctttgtctaa acgatttcta
      661 gccattaagc gctgcaattg tgtctgagca tctacataaa ccagccaaat cgcatcaaac
      721 cagtcttggt agcccaattc cattaaaatg ggaatatcca tgaaaaaaat ggcttggctc
      781 tgtgctaagt ggtcacgctt agccgccaat tcttccttaa tgatttgatt ttgaatggct
      841 gaggatgttg ccatattatc tgggtttgaa aaaagcattt ctgacagctt tgtgcgatct
      901 aactcaccat ctgcttttaa aatctgattc ccaaaagctt ctctaagagc ctcgtaaaga
      961 cgaccgcctt tttcttgcaa gtcatggacc acttgatccg catcaatcac ctgataacca
     1021 gcttttctaa tgaccttaac cacagtcgat tttcctgatg caatgcctcc tgtgatgcca
     1081 ataatcatgg tctttttttc tggcattttg gacaaatgtg cgtccccctt cctccaactt
     1141 ttaatttgac aatagcctgt ccacaacgag gacacggttt cccagtttga ccataaacct
     1201 gcaaatagtc ctgcatggtg ccatcagctc ccaaagcatt acggtaagta cggacggtcg
     1261 aaccgccctt ttcaattcct aaagctagga ttcgtatggt ttcatcatgc agccgtttta
     1321 tctccgcctt attcaaccta gaagacgcag ttcctggatg aaccttagca gcccaaagta
     1381 cctcgtccac ataaatattc cctaaaccag caaccaaggt ctggtctaac agatgtggtt
     1441 ttatgggttt ttgggaggat agcagagcag cctcaaaggt ttttaactta aaggtctctt
     1501 tctttggttc agggcctagt tttcgtttgg caaaaaacgc tgataattga gattttgcta
     1561 tcaaatcaaa ggttccaaat ttacgcacat cttggtaaac caaggtcgaa ccatttttca
     1621 attcaaaaaa gacatgaaag tgtttgttat cagggacctc atctggaaaa agcaaatatt
     1681 tgccttccat ccgcaaatgt gataccaaca ctaattgccc caaatcaatt aataaatatt
     1741 ttccccgacg gccaactgac tgaatgattt gaccaggtag ggtcagggca aacgtctcta
     1801 agtcggtttt aaccatcttt ggcactttaa gcgtaacagc cacaatttct tggccaagca
     1861 ctaaagtctc aagccctcgt cggaccgttt caacttctgg taattctggc ataatccgtt
     1921 atttaaagga cagctagacc ctttgctcct acaaagaata aactcagtat aaaaaagtca
     1981 ctcaatagac tcaggtctag gctattgact gcgtttcaaa aaacacagct gtccttctcc
     2041 tttcttattt cttattttac gaacacatct gatagaaagc aactcataaa tctgcctcag
     2101 accagtcctc atccccctta cctcccagca ctatttggcg gtaatgttcc ttataactat
     2161 aatacaatga gtcctctcca agatacttaa aaatctgcaa ggcctgccgc atgtcctctt
     2221 ctccaga
//
LOCUS       DAUVUY010000149         2122 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.150, whole genome shotgun sequence.
ACCESSION   DAUVUY010000149 DAUVUY010000000
VERSION     DAUVUY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.150"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..670
                     /locus_tag="VOX58_001910"
     CDS             <1..670
                     /locus_tag="VOX58_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HES5879109.1"
                     /translation="SKMKISEVTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDF
                     FESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAE
                     SFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPT
                     ELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISG
                     YLDK"
     gene            737..1231
                     /locus_tag="VOX58_001911"
     CDS             737..1231
                     /locus_tag="VOX58_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HES5879110.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1287..1925
                     /locus_tag="VOX58_001912"
     CDS             1287..1925
                     /locus_tag="VOX58_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879111.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1997..>2122
                     /locus_tag="VOX58_001913"
     CDS             1997..>2122
                     /locus_tag="VOX58_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HES5879112.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLF"
BASE COUNT          787 a          279 c          403 g          653 t
ORIGIN      
        1 ttctaaaatg aaaatatcag aggtaacttt agatgagact gacgatttta cttattataa
       61 aggatataaa aagggctgga atacgctttg cacatatctt aaaataagag ttccttttga
      121 tgatttagac ttttttgaaa gtcataaaga tttgattacc cagacagcaa gctccatata
      181 tgataaacaa ggtgataatg tattagtgga tactatttta gtaccacttc cagagaatta
      241 cgaagtaatt aactttagtc agttgaaaat tagtgatgtg gtttcacaag ctattgaaga
      301 cgctgagtct tttatgagta atggtgaata tcaacgtgcc tttgacagag ttcacactgc
      361 ttttcatgga tatttaattg aaatactaaa aaaatatgaa attacagttc ctagagacga
      421 aaatctatct aaattatata gtaggataca acaattaatt gaaaaagaaa ttcaacctac
      481 tgaacttgct gatatagtga agacaacgat aaggtcatca aatggaatga ttagctctct
      541 taatgaagca agaaataggc attcacttgc acaccctaat acaaatatta ttggaaagag
      601 agaagcaaaa ctaataatag gtatttcaag tacagttaca gattatatta gcggatacct
      661 tgataagtag cgtcattaac tgattgctac tttttttatg actattttta taaaaaggat
      721 gtgataagaa ttggaaatga aagtctatgt ggcaaattta ggacggtata atgaaggtga
      781 attagtcgga gcatggttta ctccaccaat tgatgaggaa gaaatggcag aaagaatcgg
      841 tttaaatgag gactacgaag aatatgcaat acatgatttt gagcttcctt ttgatgtgga
      901 cgaatacacg ccgattagtg aaatcaaccg tctatgtgaa gccattcaag aaatcgaagg
      961 tacacctatt tataatgaac tcaaagaaat tcaaggcatg tggtttagta gcttggaaga
     1021 attactggaa aacaaagagg atattcattg ttatagtgat tgtgattcga tggaagatgt
     1081 cgcccgttat tacgtggaag aaacaggaca gcttggtgaa gtcccttcca atttacaaaa
     1141 ttatattgac taccaatctt tgggacgtga tatggaaata gagggtaatt acctagttac
     1201 ctctcatggg gtatttgaat attgtcagta agtttaacaa tccgtgttat aattaaatta
     1261 ataaatagca cggagaggta taaattatgg taaatgtatt tgaaaatctt aaactattaa
     1321 aactagacat gaaagataaa ggctgggtga tagattcctt ttactttcgt tacaaacagc
     1381 aaaattatat tgtacttgtt aagctatttg aaaaagagga aaaagttcca gaatatgctc
     1441 tgttaaaact agaattttta aaggaaaacg atttttcaga tatgctagct gtctatgcta
     1501 attcggtaaa attatataca gacacgaaaa caataagaga atattttggt atcgaataca
     1561 gtagtaactt gggagatgtt ttatttcaat ttagccaaac tcttgctcga tttattccga
     1621 cagaagttag tgagaagaaa aatgaagacc aaaaagaagc catgtgtttc tcattaagtc
     1681 aaagtgattc agaagatccc agaaaaaaat attgtttttc agttagaaga aatcctttaa
     1741 agggaaatgg tgagctggga aaacgaagtc cttttaatga caataaaacg agattgtata
     1801 gacccagctt atatgaacga ttagggtcag ataccaattt aagtttcaga tattcaatga
     1861 atccagatga tgaagaaact gatgaaggta ttattgcaaa atggacaaaa aataaaattg
     1921 aatagttaaa gcattgaaag agcaatcacc tattttgatt gttctttttc tatttttacg
     1981 gaaaggacgt gaagacatga aaaaaataag aagttacacc agtatttggt cagtagaaaa
     2041 agtgatttat gcgattaatg atttacaact ccctttccca attaccttta cgcaaatgac
     2101 atggtttatc gtttctttgt tt
//
LOCUS       DAUVUY010000150         2103 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.151, whole genome shotgun sequence.
ACCESSION   DAUVUY010000150 DAUVUY010000000
VERSION     DAUVUY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.151"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            25..339
                     /locus_tag="VOX58_001914"
     CDS             25..339
                     /locus_tag="VOX58_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879113.1"
                     /translation="MTRLISQQILSEYVKSLEVEADKVVIPGQNTPVFSWDRDGNGNL
                     SIDTPLLKVRGESLATQADLTTISLTPGPKGELWQTSVERLDIKVDKHISLTFKNGVR
                     ID"
     gene            756..1094
                     /locus_tag="VOX58_001915"
     CDS             756..1094
                     /locus_tag="VOX58_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HES5879114.1"
                     /translation="MKPPFTVTNTMLNKVVEISKIIGNLELQVQKDLKLRKENRIQSI
                     HSSLAIEQNSLTVEQITAIIDGKRVLGNPREIREVKNAYEAYEEILTLTPYDESHFLK
                     MKEFQQYIYR"
     gene            1118..1989
                     /locus_tag="VOX58_001916"
                     /pseudo
     CDS             1118..1989
                     /locus_tag="VOX58_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528389.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine ligase"
BASE COUNT          754 a          260 c          443 g          646 t
ORIGIN      
        1 ttgaccgtct ctagttcagc atggatgaca aggcttatct cccaacagat tctatcagag
       61 tatgtgaaga gcctagaagt tgaagctgat aaggtcgtta ttccaggaca gaatactcca
      121 gtctttagtt gggataggga tgggaatggg aatctttcca ttgacacacc gctcctaaag
      181 gtgagagggg aaagtctagc gacacaagct gaccttacaa ccatctcttt aactcctggt
      241 ccaaagggag aactctggca gacaagtgtt gaaaggttgg atataaaagt agacaagcac
      301 atcagcctaa ccttcaaaaa tggcgtacgg attgattgat aaaaggcaag ggcgtatcaa
      361 ttcggcacgt ccttttttag tttatctgtg gtataatgaa agaagttata ttgggagttg
      421 agatagacga ttcatttgaa ttttgcactc cctaaaaagg gtaaaggtta gattgtatga
      481 taaaactaat agcaacagaa ataggaataa agtggataat acaataaaag ttctaaatag
      541 cttgaaacaa agggactagg agtttgagtg ggagttttaa tactctaggg taattttatg
      601 tagagtattt gaaaataata acgttaaaaa gttagtaatc gttaaagaat ttaaaataaa
      661 ttgtaatagt ttatcgcttg ttttatcgct tgaagaacga gcgataaatt tatttttatg
      721 gtataaataa gaaaaaagat tgtaaggaga aaatgatgaa accacctttt acagttacga
      781 ataccatgtt aaataaggta gtagagattt ctaaaattat tggtaattta gaattacaag
      841 tacagaaaga tttaaaatta agaaaagaaa atcgtattca gtctattcac tcatcactcg
      901 ctattgaaca aaattcttta acagtagaac aaataacagc cattattgat gggaaaagag
      961 tgttagggaa cccccgtgag ataagagaag taaaaaacgc ttatgaagct tatgaagaaa
     1021 ttttaacgct aactccctat gatgaatcac attttttaaa aatgaaggaa tttcaacagt
     1081 acatttaccg gtaacaacat cgtcaatcac aagtcctatg gggttgggga gtgattcgct
     1141 acctgtaaaa atttccttat ttgatgttga tacctatctt gcagattcga tgcagtttca
     1201 cttagagtat atacttagac tttttccaaa gtcaggagtt catcacttaa tgcctacttt
     1261 tagaggagaa gcagtagatc atcgacattt atcgcaattt tatcatttag aggagaagca
     1321 gtagatcatc gacatttatc gcaattttat catagtgagt gtgagataga gggagattta
     1381 cacgatgtga tgaatttagt ggatagatat attaagtttc tggttagtga aattcttaga
     1441 gagtgtcgag atagtatttt tgctataaca aaagatgtaa gtcatttgga aagttttatt
     1501 gatcgaaaga taccaattat tacgtttgaa gaggcttgta ctatattgga aaatgaaata
     1561 gattcattgg aatataatga tgaacatcat ttttatgtgt taacagatgt tggggaaaag
     1621 aaattaatgg aacattttga gggagtagtt tgggtaaaaa attatccctg taaaagtgtc
     1681 cctttttatc aaaggttctc aacagatcat ctatttgcct ataatgcaga tttgttgtta
     1741 ggaataggcg aaactgtagg ttgtggagaa agatgcgaaa catatgaaga agttgtagaa
     1801 aatatgaatt atttacaagt agatgcttct gaatatagtt ggtatttaga aatgaaaaaa
     1861 acaagaccta tgaaatcttc aggatttggt ttaggagtag agcgtttaat agcgtggata
     1921 ttgaagtgcg atgatgttcg aaaagttcaa ttaatcagta gagaaaatgg gattgattat
     1981 ttaccataaa ggaggaagaa aaatgaaaaa gaatatttta attgtaggtg gaggtccaga
     2041 gataactata tgttatgatt taattaacaa tagtacggag tataatttct tttcagtatc
     2101 aga
//
LOCUS       DAUVUY010000151         2101 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.152, whole genome shotgun sequence.
ACCESSION   DAUVUY010000151 DAUVUY010000000
VERSION     DAUVUY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.152"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(25..396)
                     /locus_tag="VOX58_001917"
     CDS             complement(25..396)
                     /locus_tag="VOX58_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HES5879115.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            complement(463..1014)
                     /locus_tag="VOX58_001918"
     CDS             complement(463..1014)
                     /locus_tag="VOX58_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HES5879116.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKHSDVR"
     gene            complement(1277..>2101)
                     /locus_tag="VOX58_001919"
     CDS             complement(1277..>2101)
                     /locus_tag="VOX58_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HES5879117.1"
                     /translation="GKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHASNVGISAN
                     VEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGG
                     GMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAFAGYTEVRD
                     TEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAGTIGVMDAI
                     VKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGLAALTEK"
BASE COUNT          686 a          432 c          323 g          660 t
ORIGIN      
        1 tttaaatttt atacaattca tatcttaaat acgaaaatta ccaatatgct gtgagggagt
       61 agaaaaaccg ctttgtgtca gcatttcatc atgtaagatc cgtctgacat ctgcatctgt
      121 tagagctttt tgtttttgca ttaacttacc ttgacgctcg acatattcgc gtttaatagc
      181 tgcaatattt aatccctcag acaaaaaatc tttaatttcc aaaagtcggt ccatgtcatt
      241 taaggaaaac atacgccgat tcccttgcgt tcgttcaggc ttaattaaac cttgatcttc
      301 ataatagcga atttgtctcg cagagagatc tgttaatgtc atcaccgttc caataggaaa
      361 aacagccatt gagcgtctaa gttctttttc tttcatgaca aactcctttc atcttcctta
      421 ttataggctg aaaaaaataa cctgtcaagt atatatgtta tattatctaa catcactgtg
      481 ttttttgtta aaccatttat ttttaataag ttcaacatca gtattaacta aaatagctac
      541 aaagacgcca caaaatgttt caaatacccg cgaagtcaca taaataaacg tttgtcctgt
      601 tggaatagac agtgtaataa tcaaaagagc agccactgcc ccaataatac ctgatttatt
      661 attacatgca acattaacca tgatacataa catggtacaa ataggaacaa ttaataaagt
      721 aacccaaaaa gcttgatgaa atatctcatt gaatgcaaaa aatactaagg agagtaggcc
      781 acctatacta ttaccaataa ttctagaaaa gccaaagtgc acgcttttat caaagtcttc
      841 tcttaaacta aatacagcag ttagagcacc aatttgaagc cctttccaac caaataaatg
      901 aaaaaccaat aaaactaaaa agaccgataa gcctgttttc aatgttctca ttcccaattt
      961 aaacttttta atatcaaact tgtaattaga taataatttt cttaacgttt tcatacctat
     1021 cccttcacta tctattttaa catatcaatc cataaaaccc ttttggtaaa cgttttattt
     1081 atgattagga tatcttttat aaaccaattg atatgaaatc gtcataaact tttgaaaatt
     1141 gcagtgtcag ccaaatacca ctatatctaa aaactagtaa atgataagtg aagttaggga
     1201 aagttcaata actgtacatg atcaactaaa aaaagacatg catcagcatg ccctttacta
     1261 tatattataa tgactattat ttttcagtca atgctgccaa acctggtaat actttaccct
     1321 cgagaagttc catgctagcg cctccgccgg tactgatcca tgagaatttg tcagcacggc
     1381 caaggttgat agcagctgct gctgaatcac caccaccgat gatggattta acgcctggtt
     1441 gtttaacgat agcgtccatg acaccgattg taccagcttg gaagtcaggg ttttcaaaga
     1501 cacccatagg tccgttccaa acaactgttt tagcaccagt aagtgcttca tcaaatttag
     1561 cgattgattt aggaccgatg tcaagaccaa ggaagccttc tgaaactgct tcaccttcag
     1621 tgtcgcgaac ttcagtgtaa ccagcaaatg cgtttgcttc tttcgagtca actggcaaga
     1681 tcaatttacc atttgatttt tcaaggaggt ctttagcaac atccaatttg tcttcttcta
     1741 caagtgagtt accgatttcg ataccttgag ctttgtagaa tgtgtaagtc ataccaccac
     1801 cgataagaac tttatcagct ttttcaagaa gattttcgat aacaccaatc ttatcagaaa
     1861 cttttgagcc accaagaata gctacgaatg gacgttctgg agtttcaact gcttcttgga
     1921 tgtaagcaat ttcgttttca agaaggaaac cagctacagc tttttcaacg tttgctgaaa
     1981 tacctacgtt tgatgcgtga gcacggtgtg ctgtaccaaa tgcatcgtta acgaagattc
     2041 catctccaag tgaagcccag tatttaccaa gttcttcgtc attcttagat tctttcttac
     2101 c
//
LOCUS       DAUVUY010000152         1936 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.153, whole genome shotgun sequence.
ACCESSION   DAUVUY010000152 DAUVUY010000000
VERSION     DAUVUY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.153"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..1336)
                     /locus_tag="VOX58_001920"
     CDS             complement(<1..1336)
                     /locus_tag="VOX58_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HES5879118.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVTQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNTFLEGINFAEIMIIVLDECRDAKLKLSDFVM
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLTTRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRHKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYE"
     gene            complement(1442..>1936)
                     /locus_tag="VOX58_001921"
     CDS             complement(1442..>1936)
                     /locus_tag="VOX58_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 family protein"
                     /protein_id="HES5879119.1"
                     /translation="HRHLPIGLQVELMKAIGTIDNILISNQFISEEELAACTQALARP
                     VTTIKVRPIIDLTEVEEKIIGYPHCYRGDVSDYVIRSTMPRLVYAQESIAPRDQSKEV
                     KRGSIIIDNDRYHRYKGELQIALKNFTVSSKANVVAEVREDYLSLLDDLRPWQEFCLE
                     IDPS"
BASE COUNT          596 a          418 c          326 g          596 t
ORIGIN      
        1 tttcatagta actgataaca tcgacaatac gcaaacgtcc ttcaaattcc ttttccaagc
       61 gatttttcaa catcatggca ctaccataac ctgtggcaca aactactaga accctcaatt
      121 tatgacggtt tgaataacgc tcaatggccg ccattaaatg caaactaata taagcccatt
      181 catcatctga gacatcattc ttttgacata caagcaaatc cgaaaaggtc tgcttggtaa
      241 gggtaaatat ttcaggatac tggcttttaa tttcctcagt taaaggatta gtcagctgaa
      301 tatggttttc aagacgtgtg gtcaagggca tcatgtgggc taataaacct tgaaataaat
      361 tggtatctgt ttctaaagtc atgcctgtca attgactaat cttggcaata catgctttta
      421 aatggtcctg caacttttca tcggtattat catcttgcca atgcttacca acagaatgtt
      481 tgacctttag atgcagtgca atataattag cctcctcttt aggaaaacgt atccccatca
      541 cttcttccac tcgatacaaa atgcgcagag ctacttgata ctcatcagac tgacgaatag
      601 ctattggaat ggaaaacagt tctaatggac accccgatcg aatacgctga accattaggg
      661 caatatgcaa gactagatta ttcatgacaa aatcagaaag tttcaactta gcatcccgac
      721 attcatccaa gacaataatc atgatttctg caaaattaat accttcaaga aaggtattac
      781 ctaccatggt ggacaaggtg ttatcaaaac tagtaacaaa aaaataatcc atgataaaat
      841 gcctaatgtc ctcttcttta cctcttattt gaatatgttg gtgagttatt tcaagctcta
      901 actggtaagg tattatgcgc tccttaatgc ctgctaaaac atgttttaaa gttgtccgac
      961 tgatatacaa ttcttgacaa agctcttcaa aatcctgaac ggcatcttca aaaaagaatt
     1021 tatgaagcac ataatcttct cgatcaaccg attcttccac ttgagtcaca tctgctaaac
     1081 gttttttaga cgtcacactt tcttgccaaa agatgtcaaa tgccatagag tggtcaattt
     1141 ctaagcagta acctcgacct tgtttagaaa tgatgtgagc tccgtgttca ggaaggctac
     1201 tgatcagttc tttgaggtat ttcctaaccg tacggtcact cataccaata acctcagcca
     1261 gagttgcact agagacaaac tgctctttgt gatggattag aaatgttatg atggccagtt
     1321 ctttttttga taacattagt cctccttact actttagaaa acgctctcta ccctctttta
     1381 gtgtaccaaa tatttcctga aaaaggtgac catcattttc cgcattgatt gtgaaaagaa
     1441 tctatgatgg gtcaatctcc aaacaaaatt cctgccaagg tcgcaaatca tctaacaaac
     1501 taagatagtc ctctctgacc tcagcaacca cattagcttt tgaactaacc gtaaaatttt
     1561 tcaaagcaat ttgcagttct cccttatatc gatgatagcg gtcattgtca ataataatgc
     1621 tacctcgttt gacctcctta gattggtctc ttggagcaat cgattcctga gcataaacta
     1681 gacgtggcat ggttgatcga atcacataat cagatacatc gccacgataa caatgcggat
     1741 agccaatgat tttctcctca acttctgtca aatcgatgat aggcctaacc ttgatcgttg
     1801 tgactggtct tgctaaagcc tgagtacatg ctgccaattc ttcttccgaa atgaattggt
     1861 tagatatgag gatattatca atggttccta tcgctttcat taattctact tgcaggccaa
     1921 tgggcaagtg cctatg
//
LOCUS       DAUVUY010000153         1908 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.154, whole genome shotgun sequence.
ACCESSION   DAUVUY010000153 DAUVUY010000000
VERSION     DAUVUY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.154"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            224..844
                     /locus_tag="VOX58_001922"
     CDS             224..844
                     /locus_tag="VOX58_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HES5879120.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
     gene            complement(1101..>1908)
                     /locus_tag="VOX58_001923"
     CDS             complement(1101..>1908)
                     /locus_tag="VOX58_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HES5879121.1"
                     /translation="IIDTQIKRFHEYKRQQMNALYVIHKYLEIKKGNLPKRKITVIFG
                     GKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNVHLVENYNVTVAEHLIPATDISE
                     QISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAELAGMENIYTFGKDSDTIINLY
                     ATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEERLDRLYKELISKDWFMTLIDLE
                     EYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSDRTIEQYNEDIWHSR"
BASE COUNT          623 a          355 c          317 g          613 t
ORIGIN      
        1 ccgttatccc tatagccata ccatcacgct taccgatact ccatcaatac cactcaaaac
       61 cttgtcatat cagatctttt tgaccatttt tttcatgaat atctaaaaaa aagaatcaaa
      121 atagtactaa attccccata gcgcatgcgc tatggggaat catagctata tcttgtttat
      181 aatttatgat ataatacagc aaaaatagtt taggaaataa catatgacaa aaaaacactt
      241 actaacactt cttctcatct ctttttttac tagctttttg gtagcttgtt caacaacgaa
      301 agataaagag cctcaaccgt ctgattcaga aatcattact ccccgactac accaagccgc
      361 tcatcaagat aaacgcgcta actttgaaaa aattaaactt gcgactgttg attcctcatt
      421 tacaggaggg acaagccttg aagaacttat ttcactcttt ggagagccta gccaacatga
      481 tccaaaaaca gcaggcgaag taacaatcga cgcttatact tggcagtttg atcaagttac
      541 tctcactgtt aatctttatc aaaatagtag tattgttaaa accatctcta attttacctt
      601 tgcaagagag ttaggcttat cgcaaaagga ataccaacaa ttacaaaaag gaatgtctta
      661 tgaagacgtt aaaaagatct taacagaacc tgataattat agccaagcgt catctagtga
      721 tcatcaaact ttgcaagcga tttgggttag tggcttaaag acagatacaa gcggagctaa
      781 tatttctctc gtttttgaaa ataatcagtt aacagaaatg tctcaggtag gacttgaaga
      841 ataaaaaatg agtaatatat tttgctaaaa agattgattt cgatgtacac cgaaatcaat
      901 ctttttatat cattttaata aaaaacttta gtgtaatttc ttataattgc cttcatagat
      961 cgataaacag acttgtttta cgcttaatgt aataattaat ctaagtctga agaaaaatca
     1021 taagacagca gaaactactg tcttatgatt tagtttaagc atgttacggt accgctaccc
     1081 cacattgcaa ttattatttt ttatcgtgaa tgccaaatat cttcgttata ctgctcaatg
     1141 gtacggtcag atgagaagaa tccagctttt gcgatgttat ggaccacttt tgtcatccat
     1201 aaatcttggt cttcatagtc tgctaacatt ttttctttca cttcaatgta ctcttcaagg
     1261 tcaatcaaag tcatgaacca gtcttttgaa atcaattctt tataaagacg atcaagacgt
     1321 tcttcgttgc caaatgctag caattctgga ctgataataa agttcactgc tgctttaata
     1381 gcaggatggt tatcatagta atcttttgct acataagaag cagtcgcata aaggttgatg
     1441 atggtgtcag aatctttacc aaaggtgtag atattctcca tgcctgcaag ctcagcgatt
     1501 tctacgttag caccgtccat tgtaccaagt gttaaagcac cgttaagcat gaatttcata
     1561 ttaccagttc cagaagcttc tttggatgct agtgaaattt gctcagaaat atcagttgct
     1621 ggaatcaagt gctctgctac tgtcacgttg taattttcaa ctagatgcac attaaggtat
     1681 gggcttactt cagggtcatt gttaatcaat tcagacaagc aaaggatcaa gtgaatgatg
     1741 tcttgagcaa tgatgtaagc aggcgctgct ttacctccaa agataacagt gattttacgt
     1801 tttggaaggt tgcctttttt aatttccaaa tatttgtgaa tcacgtaaag agcattcatc
     1861 tgttgacgtt tgtactcgtg gaaacgtttg atttgggtat caataata
//
LOCUS       DAUVUY010000154         1902 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.155, whole genome shotgun sequence.
ACCESSION   DAUVUY010000154 DAUVUY010000000
VERSION     DAUVUY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.155"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..842)
                     /locus_tag="VOX58_001924"
     CDS             complement(<1..842)
                     /locus_tag="VOX58_001924"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HES5879122.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTKAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDAT"
     gene            complement(1378..>1902)
                     /locus_tag="VOX58_001925"
     CDS             complement(1378..>1902)
                     /locus_tag="VOX58_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HES5879123.1"
                     /translation="IVRVEAEGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTA
                     LDILSVSKNQLTNVNLSKPNKTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYE
                     GSMVGNGTAEEKAAMATKAKESAQEASESHDYNHNHAYEDEEGHAHEHRDKDDHDHEH
                     EDENEAKDEQNHAD"
BASE COUNT          523 a          416 c          350 g          613 t
ORIGIN      
        1 tttgtcgcat cttttcctga aaaagcaccg ccaccatgac gtgaatagcc tccataggta
       61 tccacaataa tcttacgtcc ggtcaatcca gagtctcctt gcggacctcc aataacaaaa
      121 cgtcctgttg gattaatgaa gaactttgtg tcatcatcta aataatctgc tggaattaca
      181 gccttgatca ctttttcaat cacatcctga cgaatttggt cattagtagc ttctggatca
      241 tgctgagtag aaataacgac tgtatccaca cgaacaggtt tatcgtgttc atcgtactca
      301 actgtcactt gagatttagc atctggacgt agataactaa tctcacctga cttacgtaat
      361 tccgccaagc gacgcactaa ttggtgagat aaggaaattg gtaaaggcat caattctggt
      421 gtttcattga tggcaaaacc aaacatgagc ccttggtcac ccgctccaat atggctaagg
      481 tcatcagtat caccttctct gctttcgaga gcttcattaa ccccctgagc gatatctcca
      541 gattgttcta caagtgatgg atgtactccc actgattctg ctgaaaagcc atattctgct
      601 tctgtataac caatttctgc aatggtgtcg cggaccacac ggttaatatc aatgtatgct
      661 tttgttgaga tttctccaaa aacatgaaca gaacctgtgt aaacacaagt ttctgcagcc
      721 acatgggctt ctggatcttc tgctaaaatg gcatctaaaa tagcatctga aatttggtct
      781 gcaatcttat ctggatgccc ttcagatacg gactcagacg tgaaaagttt acgttctgac
      841 ataaaaatgt cccccctaat atgttgattc ttcctttttc agtcacattg accacaaagc
      901 aagaagttgc ctaccccttt cttcctagct gattgagcaa gtcaagtggc caaaaccaac
      961 ttagctaatc agttacggtt ttactagtaa ggcaactagc tagtccacct agtggctagc
     1021 gtccattaac cagacaaaga atgtctgaca atagcgttga gttacaaaga atccggtttc
     1081 cttgttgaga ggatgtcacc acaaaataag ccttagacga agtaatttag ctaaagagag
     1141 aacgactgtt ccccaaatga agtcaacaac gtctcaaaac atttcgttca acagaccgtc
     1201 ttatcgggac tcaataacaa tctattataa catgttttag ccatttaggt gccattcttt
     1261 tcactcatta tcctatttat ttcaaaaaaa cctcaaggtt attcttgagg ttttcttgat
     1321 agtcaacaat ggtgtcatta agacgattca gttggatcgg gttattctta gattagttta
     1381 gtcagcatgg ttttgctcat ctttagcttc attttcatcc tcatgttcat ggtcgtgatc
     1441 atctttgtct ctgtgctcgt gagcatgacc ttcttcatct tcataggcat gattatggtt
     1501 gtagtcatgt gattccgatg cttcttgagc actttctttc gccttagtag ccatagctgc
     1561 tttttcttca gctgttccat tacctaccat agaaccttcg taaaccgctg ggaagttttt
     1621 cgcaatggct tctggaatat gttgctcgtt caatttaagg tctgccaatg ggatattgtt
     1681 atgactaata tcaatgttag taactgtctt attgggttta gataggttga catttgttaa
     1741 ttggttttta gacacgctta aaatgtcaag tgctgtaaaa ttattaacac cttctagaga
     1801 agtcaactgg ttgcctgtca catccaagaa agtaagtgac ccttgcttgt ctttaagcac
     1861 aagcgattta atttggttac cttctgcttc tactctgaca at
//
LOCUS       DAUVUY010000155         1845 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.156, whole genome shotgun sequence.
ACCESSION   DAUVUY010000155 DAUVUY010000000
VERSION     DAUVUY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.156"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(73..145)
                     /locus_tag="VOX58_001926"
     tRNA            complement(73..145)
                     /locus_tag="VOX58_001926"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Ala,seq:tgc)
     gene            complement(197..1745)
                     /locus_tag="VOX58_001927"
     rRNA            complement(197..1745)
                     /locus_tag="VOX58_001927"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          396 a          544 c          405 g          500 t
ORIGIN      
        1 tgtctatttt cctaggaagt gagttgcgtg ttcaacttca cttgtattaa gtttagtagg
       61 attgtatcct gatggagcct agcgggatcg aaccgctgac ctcctgcgtg caaagcaggc
      121 gctctcccag ctgagctaag gccccacaag acctctcaaa actaaataag acgataaacg
      181 tgttccattt ccttagaaag gaggtgatcc agccgcacct tccgatacgg ctaccttgtt
      241 acgacttcac cccaatcatc tatcccacct taggcggctg gctcctaata ggttacctca
      301 ccgacttcgg gtgttacaaa ctctcgtggt gtgacgggcg gtgtgtacaa ggcccgggaa
      361 cgtattcacc gcggcgtgct gatccgcgat tactagcgat tccgacttca tgtaggcgag
      421 ttgcagccta caatccgaac tgagattggc tttaagagat tagcttgccg tcaccggctt
      481 gcgactcgtt gtaccaacca ttgtagcacg tgtgtagccc aggtcataag gggcatgatg
      541 atttgacgtc atccccacct tcctccggtt tattaccggc agtctcgcta gagtgcccaa
      601 cttaatgatg gcaactaaca ataggggttg cgctcgttgc gggacttaac ccaacatctc
      661 acgacacgag ctgacgacaa ccatgcacca cctgtcaccg atgtaccgaa gtaaaactct
      721 atctctagag cgggcatcgg gatgtcaaga cctggtaagg ttcttcgcgt tgcttcgaat
      781 taaaccacat gctccaccgc ttgtgcgggc ccccgtcaat tcctttgagt ttcaaccttg
      841 cggtcgtact ccccaggcgg agtgcttaat gcgttagctc cggcactaag ccccggaaag
      901 ggcctaacac ctagcactca tcgtttacgg cgtggactac cagggtatct aatcctgttt
      961 gctccccacg ctttcgagcc tcagcgtcag ttacagacca gagagccgct ttcgccaccg
     1021 gtgttcctcc atatatctac gcatttcacc gctacacatg gaattccact ctccccttct
     1081 gcactcaagt tctccagttt ccaaagcgta cattggttga gccaatgcct ttaacttcag
     1141 acttaaaaaa ccgcctgcgc tcgctttacg cccaataaat ccggacaacg ctcgggacct
     1201 acgtattacc gcggctgctg gcacgtagtt agccgtccct ttctggttag ttaccgtcac
     1261 ttggtggatt ttccactccc accatcattc ttctctaaca acagagcttt acgatccgaa
     1321 aaccttcttc actcacgcgg cgttgctcgg tcagggttgc ccccattgcc gaagattccc
     1381 tactgctgcc tcccgtagga gtctgggccg tgtctcagtc ccagtgtggc cgatcaccct
     1441 ctcaggtcgg ctatgtatcg tcgccttggt gagcctttac ctcaccaact agctaataca
     1501 acgcaggtcc atctcatagt ggagcaattg ccccttttaa attactaaca tgcgttagtc
     1561 tctcttatgc ggtattagct atcgtttcca atagttatcc cccgctatga ggtaggttac
     1621 ctacgcgtta ctcacccgtt cgcaactcct tgaaccggtg caagcaccag ttctcagcgt
     1681 tctacttgca tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctct
     1741 ttaatttgcg tttgaactta ctcgttcctt ctgtcgctga cagatttatt tctcgtttct
     1801 tgacgggtaa tatgtctcca tatcaccctc acgtttggtt cgtct
//
LOCUS       DAUVUY010000156         1842 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.157, whole genome shotgun sequence.
ACCESSION   DAUVUY010000156 DAUVUY010000000
VERSION     DAUVUY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.157"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..842
                     /locus_tag="VOX58_001928"
     CDS             <1..842
                     /locus_tag="VOX58_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HES5879124.1"
                     /translation="DISRPGLEMTGYFDYYAPERLQLFGMKEWSYLTQMTSHNRYSVL
                     KEMFKKDTPAVVVSRNLAIPKEMVQAAKEEGISLLSSRVSTSRLAGEMSYFLDASLAE
                     RTSVHGVLMDIYGMGVLIQGDSGIGKSETGLELVKRGHRLVADDRVDVYAKDEETLWG
                     EPAEILRHLLEIRGVGIIDVMSLYGASAVKDSSQVQLAIYLENFEAGKVFDRLGNGNE
                     EITFSGVRIPRIRIPVKTGRNVSVVIEAAAMNHRAKEMGFDATKTFEDRLTQLITKNE
                     VSQ"
     gene            839..1618
                     /locus_tag="VOX58_001929"
     CDS             839..1618
                     /locus_tag="VOX58_001929"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HES5879125.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            1640..>1842
                     /locus_tag="VOX58_001930"
     CDS             1640..>1842
                     /locus_tag="VOX58_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HES5879126.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDV"
BASE COUNT          529 a          300 c          400 g          613 t
ORIGIN      
        1 cagatatttc acgtccaggt cttgaaatga ctggttattt tgattattat gctccagaaa
       61 ggttgcagct atttggaatg aaagagtggt cttatcttac acagatgact tctcacaacc
      121 gttattctgt tttaaaagag atgttcaaaa aggacactcc tgcagtggtt gtctctagaa
      181 atttagccat tccaaaagaa atggttcaag ctgcaaaaga agaaggtatt tcacttttaa
      241 gtagccgtgt ttcaactagc cgcttagctg gagaaatgtc ttattttctt gacgcttctt
      301 tagctgaacg tactagtgtt cacggcgttt taatggatat ttatggtatg ggggttctta
      361 tccaagggga ttctggaatt ggtaaaagtg agactggttt agaattagtt aagcgagggc
      421 atcgtttggt tgcagatgac cgtgtggatg tctatgccaa agatgaagaa accctttggg
      481 gagagccagc agagatcttg cgccacttgc ttgaaattcg tggggtaggt attattgatg
      541 tgatgtcgct ttatggagct agtgctgtga aggactcatc acaagttcaa ttggccattt
      601 accttgaaaa ctttgaggct ggtaaagttt ttgatcgtct cggaaacggt aatgaggaga
      661 taactttctc aggagtgaga atccctcgta ttcgtattcc ggtaaaaaca ggtcgaaatg
      721 tttcagtggt tattgaagcc gctgcgatga atcatcgggc taaagaaatg ggctttgatg
      781 ctacaaaaac ttttgaagat cgtttaactc aactaatcac caaaaatgag gtgagtcaat
      841 gatcaatccc attgccttaa aatgtggtcc actagccatc cactggtatg cgctatgtat
      901 cttgtcagga cttgttttgg cggtttactt agcatcaaaa gaagcaccca aaaaaggaat
      961 atcatctgac gctattttcg attttattct gattgctttt ccacttgcta ttgttggtgc
     1021 aagaatttac tatgttattt ttgaatggtc ttattatgtc aagcatttag atgaaatcat
     1081 agccatttgg aatggcggta ttgctattta tggcggtctc attacaggag ctcttgtact
     1141 tttggcttac tgttataaca aggtgctcaa tcccattcat tttttagata ttgccgcacc
     1201 aagtgtcatg gtcgctcaag ctatcggtcg ctggggaaat tttatcaacc aagaagccta
     1261 tggtaaagct gtgagccagt taaattactt accaagcttt attcaaaagc aaatgtttat
     1321 agagggaagt taccgcattc cgacctttct ctatgaatca ctttggaatc ttttgggatt
     1381 tgtcattatt atgatgtggc gtcgtaagcc aaaaagtcta ttagatggag aaatctttgc
     1441 attttactta atttggtatg gtagtggaag actagtcatt gaaggtatgc gaacagatag
     1501 tcttatgttt ttaggtattc gcatctccca atatgtgtct gccttattaa ttattattgg
     1561 cttaatcttt gtcataaaaa gacgccgtca aaaaggaatt tcttattatc aagaataagg
     1621 atagaaaaag gagaaactta tggacttagt tggaatctca ctgatgatta ttgcactcgc
     1681 atttgttgct ttggtgattt tcttgattat tgtattaaag aaagtctcag aaacaattga
     1741 tgaagccaaa aaaacgattt ctgtattgac aagtgatgtt aatgtgacac ttcatcaaac
     1801 taatgatatt ttagcaaaag ctaatatcct tgttgaagat gt
//
LOCUS       DAUVUY010000157         1800 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.158, whole genome shotgun sequence.
ACCESSION   DAUVUY010000157 DAUVUY010000000
VERSION     DAUVUY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.158"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(217..1473)
                     /locus_tag="VOX58_001931"
     CDS             complement(217..1473)
                     /locus_tag="VOX58_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HES5879127.1"
                     /translation="MRRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDSMQPI
                     SSTQGNRANTSKKWYDINEYGLIPVDISFEAAKKDFKEEVEKSLLSEEQQKTYNQKIE
                     AARNKDDLLLTYHSEYSKDIKKHSTSTEPVEASVQETQASVSDSMVTDDSTSVTADSP
                     EKTPSTETPAPSIPEVPEAPASSPESEESSVASSEETSSPETPVAPEAPALSEAPAQP
                     AESEESSVAAPSEETPSPETPAAPETPEEPAAPSPSPESEEPSVAASSEETPSPETPA
                     APETPEEPAAPAQPAESEESSVAATTSPSPSTPAESETQTPPAVTKDSDKPSSAAEKP
                     AASSLVSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVRAHESGKYLPSTGEKAQP
                     LFIATMTLMSLFGSLLVTKRQKETKK"
     gene            complement(1570..>1800)
                     /locus_tag="VOX58_001932"
     CDS             complement(1570..>1800)
                     /locus_tag="VOX58_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879128.1"
                     /translation="DSSGQTPGKTPQKGQPSRALEKRSSKRALATKASTRDQLPTTND
                     KDTNRLHLLKLVMTTFFLGLVAHIFKTKRTED"
BASE COUNT          445 a          340 c          409 g          606 t
ORIGIN      
        1 aaaagtttct tcatgcgttt ggcgtgccat cttttatcaa tatatgataa aatcacagca
       61 actgaagaga taagcaaaag acaattgttg acagctattt gtcctaactt ctttttgatg
      121 aggcagcaca tctaaaaaaa tagagagaac caaattggtt ctctctatta aataagctct
      181 tatcagttta agtaatacta ttcagtttaa gtaatactat tttttagttt ctttttggcg
      241 ttttgtgact aaaagactgc caaatagaga catcaaagtc atagtagcta taaagagtgg
      301 ctgtgctttt tcacctgttg aaggcaagta cttacctgac tcatgggccc taacctgtct
      361 tgacaatgag cgatttggag agtaagactg ttcttgctct tcttttttat cagaagatct
      421 ctttgaagtt ggttgttgaa cggtttgttc tgaaacaagt gaagaggctg ctggtttttc
      481 agctgctgaa gatggcttat cagagtcttt agtaacagct ggtggcgtct gagtctctga
      541 ttcggctgga gttgatggag acgggcttgt cgtagctgct actgaagatt cttcactctc
      601 agctggttga gctggagctg ctggttcttc aggagtttct ggagccgctg gagtttctgg
      661 agatggggtt tcctcagaag aagctgctac tgaaggttcc tcactctcag gtgatggaga
      721 tggagctgct ggctcttcag gagtttctgg ggccgctgga gtttctggag atggggtttc
      781 ctcagaagga gctgctactg aagactcctc actctcagct ggttgagctg gagcctcaga
      841 taaagctggg gcttctgggg ccactggagt ttctggagat gaggtttcct cagaagatgc
      901 tactgaagac tcctcactct caggtgatga agctggggct tctgggacct ctggaattga
      961 tggagccggt gtttctgtag atggtgtttt ctcaggagaa tcagccgtaa ctgatgttga
     1021 atcatctgtc accatcgaat ctgaaactga tgcctgtgtc tcctgcacag atgcctcaac
     1081 tggctcagta gacgttgagt gtttcttaat atctttagag tactcactat gatacgttaa
     1141 caataggtcg tctttgtttc ttgcagcttc aattttttga ttatacgttt tctgttgttc
     1201 ttcagacaaa agtgattttt ctacttcttc tttaaaatct tttttggcag cttcaaagct
     1261 tatatctaca ggaataagac catactcatt aatatcatac cattttttgg atgtattggc
     1321 tctgtttcct tgagtcgaag agattggctg catgctatct acggcatagg caacttttcc
     1381 caaaaagaga ctcgcaattg ctatactcgt taccccaacg ctcagtttgc gaatggaata
     1441 gcggctgtgt ttgttatttt ctgctctacg catattgtcc tcacatatct atttttatac
     1501 tgctatgcct tcttttgaaa agaagactga tctaactcat catcaatagt agccatctga
     1561 tcagttgtac taatcttcag tgcgttttgt tttaaagata tgagctacta atcccaagaa
     1621 gaaagtggtc ataactaact taaggagatg taaacgattt gtatccttgt cattagtcgt
     1681 tggtaactga tctcttgttg atgcttttgt agctaaagca cgcttagaag atcgtttctc
     1741 tagagcacga gaaggttgac ctttttgagg agttttacct ggtgtttgac ctgaactatc
//
LOCUS       DAUVUY010000158         1798 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.159, whole genome shotgun sequence.
ACCESSION   DAUVUY010000158 DAUVUY010000000
VERSION     DAUVUY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.159"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..186)
                     /locus_tag="VOX58_001933"
     CDS             complement(<1..186)
                     /locus_tag="VOX58_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HES5879129.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSDV"
     gene            complement(824..1009)
                     /locus_tag="VOX58_001934"
     CDS             complement(824..1009)
                     /locus_tag="VOX58_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879130.1"
                     /translation="MSFEIFTLIIGWKLVGYRVMECGSSFKQGDYRLVVHICLLNVRD
                     EMLSQQGHEDKADWPEP"
     gene            complement(1269..1352)
                     /locus_tag="VOX58_001935"
     tRNA            complement(1269..1352)
                     /locus_tag="VOX58_001935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1316..1318),aa:Leu,seq:caa)
     gene            complement(1372..1443)
                     /locus_tag="VOX58_001936"
     tRNA            complement(1372..1443)
                     /locus_tag="VOX58_001936"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1409..1411),aa:Gln,seq:ttg)
     gene            complement(1452..1524)
                     /locus_tag="VOX58_001937"
     tRNA            complement(1452..1524)
                     /locus_tag="VOX58_001937"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1489..1491),aa:His,seq:gtg)
     gene            complement(1536..1606)
                     /locus_tag="VOX58_001938"
     tRNA            complement(1536..1606)
                     /locus_tag="VOX58_001938"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1572..1574),aa:Trp,seq:cca)
     gene            complement(1613..1693)
                     /locus_tag="VOX58_001939"
     tRNA            complement(1613..1693)
                     /locus_tag="VOX58_001939"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1657..1659),aa:Tyr,seq:gta)
     gene            complement(1713..1785)
                     /locus_tag="VOX58_001940"
     tRNA            complement(1713..1785)
                     /locus_tag="VOX58_001940"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1750..1752),aa:Phe,seq:gaa)
BASE COUNT          585 a          462 c          269 g          482 t
ORIGIN      
        1 cacatcacta ccaatcggta taccaatatc catcatatct tttcggctaa gagtaaggag
       61 ccataactga tttaatgctc tgatagtaac agcatcgcta gaacctcctc ccataccagc
      121 acacatggga atccgctttt ccaagtaaac ggaaactcct cttttaactt gaaatcgatg
      181 tttcattagc tgaactatct tataaacatc attatcttag ttaataggta ttttcggaca
      241 gtttgaggca aagacaatct tattgtcatc tatatggtca accgtcacat agtcacacag
      301 atcaatactc atcatgacca tcgataaatc gtggtaacca tctcatcgct tattcttagt
      361 atcaaggcct aacttgattt ttacagaagc tctctcattg attgccatca tagtactcct
      421 tacttcttac atcagtacac tatcaaatat acacaaacct cagaaaaaat tcaaaaatta
      481 ttacttctaa gattgatgga ctttatattg gccaaccatc tcaaagcaca aatttatttt
      541 atacttcaga taaggaatga acaaaccttc atcaatcata cctaaaatat cttcacgact
      601 gtctcaacga attacctgca cttcttcctc ataaagaacc aaattctcca actctacaaa
      661 gtcctgaact aaaaacgtat catcaaaccc atcatcgaaa tgaattgtaa agtgaggtca
      721 taccccagtc aagcctaatg aaagtcccaa ttcttcctca ggatctaata ttactatatg
      781 ctgtgccatc tcaccaaaaa ggacttccac caaacgtcac atctcaaggc tctggccagt
      841 cagctttatc ttcatggcct tgctgactca gcatctcatc cctaacattt aacaagcaaa
      901 tatgaaccac caaacgataa tctccttgct taaacgaaga accacactcc attacccgat
      961 acccaaccag tttccacccg attatcagtg taaatatctc aaaactcaac caccattaac
     1021 ctccaacatt agttacaata catcaatcca taactgtctt tctatagcta aaaaagcacc
     1081 tagtagtgac aaaccttttt caaaacctta ccggtaatcc cataaagcat caatactcaa
     1141 tcatattcat cataccacaa atcttgcgca ttaaatagac cttatcagta atataagaga
     1201 ttgtactacg tagctacagc ttaagatcaa aacaaaaaac aaggtttgta aaccttgttt
     1261 ttcaagtata ccggcggccg gggtcgaacc ggcacgtcct tgcggacact ggattttgag
     1321 tccagcgcgt ctgccaattc cgccacgccg gctttaaata taaaatttaa actggggtag
     1381 ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc
     1441 ccaataaaat aggcgagtga tgggaatcga acccacgaat gtcagagcca caatctgatg
     1501 tgttaaccac ttcaccacac ccgccatatt tctaacacgg gcagtaggaa tcgaacccac
     1561 actgaaggtt ttggagacct tagttctacc tttaaactat gcccgtttag gatggaaaga
     1621 gagggattcg aacccccgaa cccgaaggag cggatttaca gtccgccgcg tttagcctct
     1681 tcgctatctt tccgtaaaat attaaatttt aatggcgcga gacggaatcg aaccgccgac
     1741 acatggagct tcaatccatt gctctaccaa ctgagctacc gagccaacaa ctacatac
//
LOCUS       DAUVUY010000159         1794 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.160, whole genome shotgun sequence.
ACCESSION   DAUVUY010000159 DAUVUY010000000
VERSION     DAUVUY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.160"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..863)
                     /locus_tag="VOX58_001941"
     CDS             complement(<1..863)
                     /locus_tag="VOX58_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HES5879131.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYVEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLVKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYF"
     gene            1037..>1794
                     /locus_tag="VOX58_001942"
     CDS             1037..>1794
                     /locus_tag="VOX58_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HES5879132.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAA"
BASE COUNT          551 a          326 c          374 g          543 t
ORIGIN      
        1 ttgaagtact caccatatcc agccttttca atcacttgac gagcagcagc atctacttga
       61 gcagctgtta cacctggttt gataaagtca atagccgcta gttgtgcctc taggcagaga
      121 ttgtagatat cgattttaaa ttggtctggc tgaccaacag ccacagttct ggtcatatca
      181 ctggtgtagc ctaaggtctc gacacctaag tcaaaaagaa gaagggcatt gttttcaatg
      241 ttatttgttc caggaatgcc atggggattg gctgcattat ttccagttaa gaccatagtg
      301 tcaaaactca ttttatgaat gccttgtttt ttcatttcaa actcaatttg agcgatgacg
      361 tctgtttcgg tagcatctaa ggaaatattg tcaaaaccaa cttgaacagc cttatcagcg
      421 aactgcccgg caatcatcat tttattgatt tcatcagctg atttgacgag gcgcatgcct
      481 tggacataag gggttagatt gttaaattgt cctgaaaaaa tagtctgcaa gccatggaac
      541 ttattgacgt taaggtggtc aaattcaaca taaatggttt tggctgctgt atttggtaaa
      601 acggccttga tcttttccca aggattttca gaatcaacat aaccaaacac aggaaatgat
      661 atggcctggc tagctctggc cacctcaagg gcaggtacaa aaagcacagg agctaaatca
      721 tggtagacaa acaagaacag ttgtctttca tgtgggtcac agaagaatcc agttaaatag
      781 ttaatcgtaa caggatctga gaaaatcgcc aattcagctc ccttttggtc aagatacaaa
      841 cgtatttggt ctaattttgt cataggctaa ccttctttct acttactatt ttttcaaaaa
      901 aagcttaaaa aatcaagctt cgagttaatt tttgaaaact ttttcaaaaa ttaacctata
      961 acacttgaaa gtggttacaa atcatgctaa aatacttttt gaaagcgtca ttttcaaata
     1021 gaaaggaaga taaaaaatga atacagatga taccattaca atttatgatg ttgcccgtga
     1081 agctggtgtt tcaatggcaa ccgttagtcg tgttgttaat ggcaataaaa atgttaagga
     1141 aaatacacgt aagaaagttt tagaagttat tgatcgcctt gactatcgtc ccaatgctgt
     1201 cgcgcgtggt ctcgccagca aaaagacaac aaccgttggc gttgtgattc caaatattgc
     1261 aaatagttat ttttctatct tagctaaggg tattgacgat atcgccgcta tgtacaaata
     1321 taatattgtg cttgcctcca gtgatgaaga tgatgataag gaggttaatg ttgtcaatac
     1381 cctttttgcc aagcaagtgg atggtattat ttttatgggg catcacttga cagaaaaaat
     1441 ccgggccgag ttttcacgct ctcgtactcc agttgttttg gcaggaactg tagatcttga
     1501 tcatcaatta ccaagcgtca acattgacta tagagctgcg gtgtcaaacg ttgttgatat
     1561 tttagctgaa aatcataagt gtatcgcttt tgtgtcagga ccactcattg atgatatcaa
     1621 tggtaaagtg cgcttagcag gttacaaaga agggttgaag cacaataagc ttgacttcaa
     1681 agaaggtctt gtttttgaag cgaactattc ttataaagaa ggatttgaat tggcgcagcg
     1741 cgtcattaac tcaggagcaa cagcggctta tgtggctgaa gatgaattgg cagc
//
LOCUS       DAUVUY010000160         1788 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.161, whole genome shotgun sequence.
ACCESSION   DAUVUY010000160 DAUVUY010000000
VERSION     DAUVUY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.161"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..399
                     /locus_tag="VOX58_001943"
     CDS             <1..399
                     /locus_tag="VOX58_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HES5879133.1"
                     /translation="GVLDRLSQTAQNELVNIISILLGLTISIKMQADLFLNVQTLLII
                     VFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQKMATDEDPQ
                     NFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            587..895
                     /gene="citD"
                     /locus_tag="VOX58_001944"
     CDS             587..895
                     /gene="citD"
                     /locus_tag="VOX58_001944"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HES5879134.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEKTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGVDQYDWKEIDSWNV
                     "
     gene            883..1770
                     /gene="citE"
                     /locus_tag="VOX58_001945"
     CDS             883..1770
                     /gene="citE"
                     /locus_tag="VOX58_001945"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HES5879135.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDIV
                     DVEAVIERVERENSIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTEKEIDHSKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
BASE COUNT          515 a          342 c          396 g          535 t
ORIGIN      
        1 ggtgtgctcg atcgcctgtc acaaactgct caaaatgaat tggtaaacat tattagtatc
       61 ttgcttggat tgaccatctc tattaaaatg caggcagacc tgttcttaaa tgtacagaca
      121 ctcttaatta tcgtctttgg tttacttgct tttatcatgg attctatcgg tggggtcatg
      181 tttgccaaat ttctcaatct cttccgaaaa gagaagatta atccaatgat tggcgctgcg
      241 ggtatttcag cttttccaat gtcaagtcgt gtcattcaaa aaatggcaac agatgaagac
      301 ccgcaaaact ttatcctcat gtatgctgta ggtgccaatg tctctggcca aatcgcctct
      361 gttatcgcag gtggtttgtt gctagcttac tttagctaag aaaggaagga aagcttatga
      421 caattaatat ggaccatttg attagttcct ttgagctaat ggcgcttggt atggcaggtg
      481 tctttatcgt gttaggaatt ctctatcttg tcgcagagat cctgatcaaa cttttcccag
      541 ttagtaaata acaatatcat ttaagaaaat caaggaggta aacgcaatgg atattaaaca
      601 aactgccgtt gctggttcac ttgaatcaag tgacctaatg attacagtat cccctaatga
      661 cgagaaaacc attacaataa ccctagacag tagtgttgaa aaacaatttg gcaatcacat
      721 tcgtcaactc attcatcaaa ccctagtgaa tttgaaggtg acggctgcta aagtagaggc
      781 tgttgataaa ggcgctttag attgcaccat tcaagcgaga accatcgcag ctgttcatcg
      841 cgcagctggt gttgaccaat acgattggaa ggagattgac tcatggaacg tttaagaaga
      901 acaatgatgt ttgtgccagg tgccaatgct gctatgcttc gagatgctcc tttgtttggt
      961 gctgattcaa tcatgtttga cttggaagat tctgtttcac tcaaagaaaa agacacttct
     1021 agagctctcg tccattttgc gctaaaaact tttgattatt cgagcgttga aacggttgtt
     1081 cgtgtgaatg gtcttgattc ttgtggggct ttagacatcg aagctgttgt tttggcaggt
     1141 gtcaacgtga ttcgtcttcc aaaaacagag actgcacaag atatcgttga tgtggaggct
     1201 gttattgaac gtgtcgaacg tgagaacagc attgaagttg gtcgcacacg tatgatggca
     1261 gccattgaat cagccgaagg tgttctaaat gctcgtgaga ttgccaaagc ttctaagcgc
     1321 ttgattggta ttgctcttgg ggcagaagac tatgtcacga atatgaaaac gcgtcgttac
     1381 ccagatggtc aagaattatt ctttgctcgt agcatgattt tacatgctgc tcgtgctgct
     1441 ggaattgctg ccattgatac tgtttattct gatgtcaata atacagaagg attccaaaac
     1501 gaagttcgta tgatcaaaca gttaggattt gacggtaaat cggtcatcaa ccctcgccaa
     1561 attcctctgg tcaatgagat ttatacccca accgaaaaag aaattgacca ttccaaacaa
     1621 gtcatctggg caattcgtga agctgaaagc aaaggctctg gcgttatttc cttaaatgga
     1681 aaaatggttg ataaaccaat cgttgaacgt gcagaacgtg tcattgccct agcaacagca
     1741 gcaggtgttt tatctgagga ggacatttaa agatggtaac aaataaac
//
LOCUS       DAUVUY010000161         1710 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.162, whole genome shotgun sequence.
ACCESSION   DAUVUY010000161 DAUVUY010000000
VERSION     DAUVUY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.162"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1661
                     /gene="mnmG"
                     /locus_tag="VOX58_001946"
     CDS             <1..1661
                     /gene="mnmG"
                     /locus_tag="VOX58_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HES5879136.1"
                     /translation="IQMKMLNTGKGPAVRALRAQADKSLYAREMKHTVEKQANLTLRQ
                     TMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTALRGEIILGELKYSSGPNNSLAS
                     VTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTEIQPGDDKPNHFSFMSKDAEYLK
                     DQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVGPRYCPSIEDKIVRFADKERHQL
                     FLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGLEKAEMMRTGYAIEYDIVLPHQL
                     RATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGINAALKVQGKPELILKRSDAYIG
                     VMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMRLTEIGRDIGLVDDERWKAFEIK
                     KNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTDAMTAKEFMRRPEIDYATAVSFV
                     GPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMKRMEEKRIPANIDWDAIDSIATE
                     ARQKFKKINPETIGQASRISGVNPADISILMIYLEGNGKAHRKY"
BASE COUNT          552 a          299 c          376 g          483 t
ORIGIN      
        1 atatccagat gaaaatgctt aatacaggta agggaccagc tgtacgcgct ctccgagctc
       61 aagcagataa atccctgtat gcgcgtgaaa tgaaacatac ggttgaaaaa caggctaatt
      121 tgactcttcg ccaaacgatg attgatgata ttctagttga agatggacga gttgtaggtg
      181 ttttaacagc cacaggacaa aaatttgcag ctaaggcagt tgtagtgaca acaggaacag
      241 ctctgcgtgg cgaaattatt ttaggagaac tcaaatattc ttctgggcct aataatagtt
      301 tggcatcagt aactttagca gataatttaa aaaaactcgg cttagaaatt ggcagattca
      361 aaacaggaac gcctcctcgt gtcaaagcat cttctattaa ttacgatcaa acggaaattc
      421 agccaggtga tgacaaacct aatcattttt catttatgtc aaaagatgct gagtatctca
      481 aagatcaaat tccctgctgg ttgacttata cgaatcaaac tagtcatgat atcattaatc
      541 agaaccttta cagagcaccg atgttttctg gcattgttaa aggtgtaggc cctcgttact
      601 gcccatctat tgaagataag attgttcgct ttgctgataa agaacgtcat caacttttct
      661 tagagccaga ggggcgtgat acagaagaag tgtatgttca agggttgtca acgagtcttc
      721 cagaagatgt ccaaaaagat ttgattcatt cgatcaaagg acttgaaaag gctgaaatga
      781 tgcgcactgg ttatgctatt gaatatgata ttgttttacc tcaccagcta cgtgccacac
      841 ttgaaaccaa gctaatttca ggactcttta cggctgggca aactaatgga acttctgggt
      901 atgaagaggc tgctggccaa ggacttattg ctggaatcaa tgctgctttg aaagttcaag
      961 gtaagccaga acttattttg aaacggagcg atgcttatat tggcgttatg attgacgatt
     1021 tggttaccaa aggaaccttg gaaccttatc gattgttgac atcgcgagct gagtatcgtt
     1081 tgattttacg tcatgacaac gctgatatgc gtttgacaga gattggtcgc gacattgggt
     1141 tggttgatga tgagcgttgg aaggccttcg aaattaagaa aaaccaattt gataacgaat
     1201 taaaacgtct gaacagtatc aaattaaaac cagtaaaagc aaccaacgat cgtgtgcagg
     1261 aacttggttt taaaccattg acggatgcta tgacagctaa agaatttatg cgtcgcccag
     1321 aaattgatta tgcgactgca gtctcttttg ttggtcctgc ggctgaagat ttagatgcta
     1381 aaatcattga acttttagaa acagaaatca agtacgaagg ctatattcgc aaagctttag
     1441 atcaggttgc taagatgaaa cgcatggaag aaaaacgtat tcctgcaaat attgattggg
     1501 atgctattga ttctattgca actgaggcgc gtcagaaatt taagaaaatt aatcctgaaa
     1561 caattggaca agctagtcgt atttcaggag tcaatcctgc ggacatcagt atcttgatga
     1621 tttatttaga aggaaacgga aaagctcatc gaaaatatta aatggaaatg aaattgtaat
     1681 ctcaaagcaa gtggaagctc tgtatttcag
//
LOCUS       DAUVUY010000162         1635 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.163, whole genome shotgun sequence.
ACCESSION   DAUVUY010000162 DAUVUY010000000
VERSION     DAUVUY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.163"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..1012
                     /locus_tag="VOX58_001947"
     CDS             <1..1012
                     /locus_tag="VOX58_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HES5879137.1"
                     /translation="GLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVI
                     TLTGFLDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFL
                     TVLWIVSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISA
                     IAGFFPYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDT
                     IVAIIRRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSS
                     RIGGVLLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDL
                     KH"
     gene            1134..>1635
                     /gene="sufC"
                     /locus_tag="VOX58_001948"
     CDS             1134..>1635
                     /gene="sufC"
                     /locus_tag="VOX58_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HES5879138.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADKDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFA"
BASE COUNT          434 a          257 c          360 g          584 t
ORIGIN      
        1 tggattagct atttttatgt cttttttagt agcaagtttg ggacttattc cgattgcttc
       61 taaaggcgcc atgttttttg gtcagaccta cttcagttat attttacctg tcgtgattgg
      121 tgctacagta attaccctta ctggtttttt agatgatttg tatgagttaa gtcctaaatt
      181 aaagatgttt ggtattctaa ttggtgcagt gattgtctgg gcttttaccg actttaaatt
      241 tgatagcttc aaaattcctt ttggagggcc gttgttagtt tttggtcctt tcttaacctt
      301 attcttaaca gtcctgtgga ttgtttccat cactaatgct attaacttga ttgacggttt
      361 ggatggtttg gttagtgggg tctctattat tagtttagtg accatggcta ttgtatctta
      421 tttcttttta cctcaaaagg atttcttttt gacgttaacc attttggtct tgatttctgc
      481 tattgcagga ttttttcctt ataattacca tccagctatg atttatttgg gtgacacagg
      541 agccctcttt attggcttta tgataggggt tttgtctctt caaggattga aaaattcaac
      601 ggctgtggct gtggtgacac ctgttattat tcttggtgta cccattatgg ataccatcgt
      661 ggctattatt cgacgtagtt tatcaggtca aaaattctac gagccggata agatgcacct
      721 gcatcatcga ctcttatcaa tgggctttac tcatcgagga gctgtcttag tagtttatgg
      781 tattaccatg cttttttccc ttatttcttt acttttaaat gtttctagtc gaattggtgg
      841 cgtcctcttg atgcttggac ttttatttgg tttagaagtt tttattgaag gattggagat
      901 ctggggtgaa aagcgaacgc ctttgtttaa cttgttaaag tttattggta atagtgatta
      961 tcgtcaagca atgcttctga aatggaaaga aaagaaggat ttgaaacact aaaaaagccg
     1021 agtaaggctt tttttgctat aatagatgca gtgaccatta taaaaaaagc agtgcaaatg
     1081 acactgtctt ttcttgagat ggtatagatg acagagaaaa taaggggtgt atcatgtcaa
     1141 ttcttgagat taataattta catgtttcaa tagaaggtaa agagattttg aaaggggtta
     1201 acctaactct taaaactggg gaagtagctg ccattatggg gccaaatggg acaggaaagt
     1261 ctaccctttc agcagctatt atgggaaatc ctaactacga agtgacccaa gggcaaattt
     1321 tgcttgacgg ggtcaatatt ctcgatctag aagttgatga gcgtgcgaga ctgggacttt
     1381 ttctcgccat gcaataccct tcagaaattc cagggattac caacgctgaa tttatgcgtg
     1441 cagcaatgaa tgccggtaag gcagataagg ataagatttc cgtgcgagat tttatcacca
     1501 aacttgatga gaaaatggct ttgttaggta tgaaagaaga gatggcagag cgttacctca
     1561 atgaaggttt ttctggtggg gagaaaaaac gcaatgaaat tcttcaattg ctcatgttag
     1621 agcctaaatt tgctt
//
LOCUS       DAUVUY010000163         1625 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.164, whole genome shotgun sequence.
ACCESSION   DAUVUY010000163 DAUVUY010000000
VERSION     DAUVUY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.164"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..760
                     /gene="msrB"
                     /locus_tag="VOX58_001949"
     CDS             <1..760
                     /gene="msrB"
                     /locus_tag="VOX58_001949"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HES5879139.1"
                     /translation="NRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVVRYKEDKSFNMTRTEVRSRSGNSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            848..1588
                     /locus_tag="VOX58_001950"
     CDS             848..1588
                     /locus_tag="VOX58_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HES5879140.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLQQKLDLSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKDLGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
BASE COUNT          527 a          311 c          347 g          440 t
ORIGIN      
        1 gaaccgtatt agcttgaaag aattattact gcattttttc cgaattattg acccaaccag
       61 tcttaataag caaggaaatg atcgcggtag tcaataccga actgggattt actatactga
      121 caaagcagat ttagctatta ttgatgaggt cttcaaagaa aaagccaagg attacaagaa
      181 aaaaatagtg gttgaaaaag caccattgaa acactttatt aaggcagaag actaccacca
      241 agattacctc aagaaaaatc caaatggcta ctgccatatt gacatcaacc aagcgactta
      301 tcctgtgatt gatgaaagca agtacccaaa accaagtgcc actgagatca aagcaaaact
      361 atctgcagac gaataccgag tgacgcaaaa aaacgaaact gaaaaagcct tttctaatcg
      421 ctactgggat tcttttgatg caggaattta cgtagacgtt gtgacaggag aaccgctttt
      481 ctcttcaaaa gacaaatttg aatcaggttg tggttggcca agttttagcc gccccatcag
      541 cccagacgtt gtccgctaca aggaagataa gagtttcaat atgacgagaa cagaagtgcg
      601 cagccgttca gggaattctc atctggggca tgtctttact gacggaccta aagaccaagg
      661 cggccttcgt tactgcatca atagcttgtc tattaccttt attccaaaag ctgatatgga
      721 agctaagggc tatggttatt tattatcatc tgtagaatag ggttaggcct aaaaatctga
      781 tataatagaa gaaagactag tatgagaggg ccatgcaagg ccctttataa agataaggag
      841 acaccgtgtg tactcattgt taattgtaga agacgaatac cttgtgcgcc agggtattcg
      901 ttctttggtt gattttagcc agttcaagat tgatagggtc aacgaagcag aaaatggcca
      961 gttggcttgg gacttgtttc agaaagagcc ttatgatatt gttttgacgg atatcaatat
     1021 gcccaaatta aatgggattc aactagcaga actcattaaa caggaatccc cccaaactca
     1081 tctggtattt ttgacgggct acgatgattt taactatgcc ttatctgctt tgaagttagg
     1141 ggcagatgat tacttgctca aacccttttc caaggccgat gtggaagata tgttaggaaa
     1201 gctccagcaa aaattagacc tttccaagaa aacagaaacc atccaggaat tggttgagca
     1261 gcctcaaaaa gaagtatcag caatagcaat ggctattcat gagcgattgg cagattctga
     1321 tttgacccta aaaagcttgg ctcagcagct tggttttagc cctaattacc ttagcgtctt
     1381 gattaaaaaa gatttaggga tgccctttca agattatctg gtacaagaac ggttgaaaaa
     1441 agccaagctt ctcttgttaa ccagtaatct taaaatctat gaaattgcag agcaggtcgg
     1501 ttttgaggac atgaattatt tttctcagcg ctttaagcaa ctggtaggcg ttaccccaag
     1561 tcagtataaa aaaggaggcc aggcatgaag cgttatcccc tactggtcca gttgatttcg
     1621 tatgt
//
LOCUS       DAUVUY010000164         1562 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.165, whole genome shotgun sequence.
ACCESSION   DAUVUY010000164 DAUVUY010000000
VERSION     DAUVUY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.165"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(174..1544)
                     /gene="dnaX"
                     /locus_tag="VOX58_001951"
     CDS             complement(174..1544)
                     /gene="dnaX"
                     /locus_tag="VOX58_001951"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HES5879141.1"
                     /translation="MRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENV
                     VFILATTELHKIPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIAR
                     RAEGGMRDALSILDQALSLSPDNQVTIAIAEEITGSISMLALGDYVRYVSQEQATQAL
                     AALETIYDSGKSMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMI
                     TVVTSHLPEIKKGTHPRIYAEMMTIQLAKEEGVALPSGLSSDVTTEVEGLKRELNQLK
                     QALAQLQASPSPSHAPVKPAQAKPKLTTYKIDRVKILKIMEETVQDSQQSRQYLDALK
                     TAWNEILDSISAQDRALLMGSEPVLANSENAILAFEAAFNAEQAMKRTDLNAIFGNIM
                     SKAAGFSPNILAVPKADFHHIRSEFALQLKTQKASDPTEETIEETLEIPEGFDFLVDK
                     INAVED"
BASE COUNT          452 a          329 c          304 g          477 t
ORIGIN      
        1 agattgatgc tgacttggat acccaagaaa gcatcaatct tttttggttt cacttctact
       61 attagaggat tggctagaaa aactatcttg gtatattaga taaaaaaacg ctaaaggtaa
      121 gcccaatagg cttaccttta tagcgttact ggttcattaa cagctaataa tgtttaatcc
      181 tcaacggcat taattttatc aactagaaaa tcaaagcctt ctggaatctc aagggtttct
      241 tctatcgtct cttctgtagg gtcagaagcc ttttgagttt tcaattgtaa ggcaaactca
      301 gaacgaatgt gatgaaaatc agctttaggg actgccaaaa tattaggtga gaagcctgct
      361 gctttgctca taatgttgcc gaatatcgca ttcaaatcag tacgcttcat tgcttgctca
      421 gcattaaaag cagcttcaaa agctaaaata gcattctcac tattggccag aacaggctct
      481 gatcccatta gtaaggctct atcttgagct gagatactgt ctaaaatttc attccaagct
      541 gtttttaggg catccaagta ttggcgtgat tgctgactat cttgaaccgt ttcttccatg
      601 attttcagta ttttcacccg atcaatcttg taagtggtta gtttcggttt agcctgtgct
      661 ggttttacag gagcatgtga gggactggga ctggcttgca attgtgccaa ggcttgctta
      721 agctgattaa gttcacgttt aagtccctca acttcagtcg tcacatctga tgacaaacct
      781 gatggtaacg ctactccttc ttctttagct agctgaatag tcatcatttc ggcataaatc
      841 cgaggatggg ttcccttttt gatttcaggg agatgactag taacaactgt tatcatttgg
      901 aatatacgat ctatcgagag agacaaattg gtatcaaaaa cagctgactg acgctgattg
      961 tcaccgccag ctttaaccac caataaatca cgcagatagg tcaataaatc tgtcgcaaag
     1021 cggctcatgc tcttcccact atcataaatg gtctctaagg ctgccagagc ttgcgtagcc
     1081 tgttcttggg agacatatcg aacatagtca cccagagcaa gcatggaaat agaacctgta
     1141 atttcttcgg caattgcaat ggtgacctga ttatctggtg acaagctcaa agcctgatct
     1201 aaaatagata aagcatcacg catgcctcct tctgctcgcc ttgcaatgag atttaaagca
     1261 tccacctcat aggcaatacc ttctttgtcc aaaacccagg ctaaatgctc tcgaatagct
     1321 ttttgcttaa tagctttgaa ttcaaagcgt tgcacacgag ataaaatagt ggctggaatt
     1381 ttatgcaatt ccgttgttgc caagataaag acaacatttt ctgtcggttc ttccaaagtt
     1441 ttcaaaagcg cattaaaagc ccctgttgat aacatgtgaa cctcatcaat aatataaacc
     1501 ttgtaagtcg cacgacttgg cgcataggtt gatttgtctc gaatgtcacg aatttcatca
     1561 ac
//
LOCUS       DAUVUY010000165         1549 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.166, whole genome shotgun sequence.
ACCESSION   DAUVUY010000165 DAUVUY010000000
VERSION     DAUVUY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.166"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            68..310
                     /locus_tag="VOX58_001952"
                     /pseudo
     CDS             68..310
                     /locus_tag="VOX58_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
     gene            307..729
                     /gene="fabZ"
                     /locus_tag="VOX58_001953"
     CDS             307..729
                     /gene="fabZ"
                     /locus_tag="VOX58_001953"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HES5879142.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            761..>1549
                     /locus_tag="VOX58_001954"
     CDS             761..>1549
                     /locus_tag="VOX58_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HES5879143.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAH
                     GNIIHLGERDCSLQRNNQKVLEESPSIAIGNTLRHEMGQAAVRAA"
BASE COUNT          451 a          281 c          397 g          420 t
ORIGIN      
        1 ggaagcgtcc agtccagcat cagttaagga tgttcctgta gaggagcaac cacaagcaga
       61 atcttttgta gcagaaggtg atattgttga aagtccttta gtcggtgtag cctatcttgc
      121 agctagccct gacaagccac catttgtggc agtcggagat actgtaaaaa aaggacaaac
      181 ccttgtgatt atcgaagcca tgaaggtgat gaacgaagtg ccagcgccat gtgatggtgt
      241 gattacagaa attttggtat caaatgaaga tgtcattgaa tttggacaag gattggtgcg
      301 gatcaaatga tggatattag agaaattcaa gcggctttgc ctcatcgcta cccaatgtta
      361 ttggttgatc gtgtgttaga agtgtcggac gaccatatcg ttgccattaa aaatgtgact
      421 attaatgaac ctttctttaa cgggcatttt cctcactatc ctgtgatgcc aggtgttctg
      481 attatggaag ctttggctca aacagctggt gtcttagaat tgtcaaaaga agaaaataaa
      541 ggcaagttgg tcttttacgc tggaatggat aaagtgaagt tcaaaaaaca agtcgtccct
      601 ggtgatcaac ttgtgatgac agcaaccttt atcaagcgtc gcggtaccat tgctgtcgtt
      661 gaagccaggg cagaagttga tggcaagcta gcagcaagtg gaacattaac ctttgcctgt
      721 gggcaatgag aatgattgtt ctgcggagga agaaaccctt atgtttaaaa aaatcttaat
      781 tgctaatcgt ggtgaaattg cagtgcgtat cattcgtgct gcacgagaat tagggatttc
      841 cacagttgca gtttattcgg aagcggataa ggaagctctg cacacgatat tggcggacga
      901 ggctatctgt attggtcctg cccgttccaa ggaatcctat cttaatatga attccgtctt
      961 gtcagcagct attgtgacag gagcccaagc gattcaccca gggtttggct ttttaagtga
     1021 aaactcaaag tttgcgacca tgtgtgaaga gatgaatatt aaatttatcg gtccatccgc
     1081 atcggttatg gataaaatgg gtgataaaat caatgctcgt tctgaaatga ttaaggcagg
     1141 agtgcccgtt attccagggt cagatggtga agtttataac gctcaagaag ccttagctat
     1201 tgccaataag attggttatc ctgtcatgct aaaagcatcg gcaggtggtg gaggtaaagg
     1261 gattcgtaaa gtggagacag aagcagattt ggaggcggct ttcaatgctg ccagtcaaga
     1321 agctttggga gctttcggaa atggcgccat gtatttggaa aaagtaattt acccagctag
     1381 acatattgag gtgcaaattt taggagacgc tcacggtaac atcattcact tgggagaacg
     1441 ggattgctcc ttgcaacgga ataatcaaaa agtcttggaa gaaagtccat ccattgctat
     1501 tggcaatacc ctcagacacg aaatggggca agcagcggtt agagcagct
//
LOCUS       DAUVUY010000166         1498 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.167, whole genome shotgun sequence.
ACCESSION   DAUVUY010000166 DAUVUY010000000
VERSION     DAUVUY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.167"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(440..1352)
                     /locus_tag="VOX58_001955"
                     /pseudo
     CDS             complement(440..1352)
                     /locus_tag="VOX58_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811473.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
BASE COUNT          403 a          330 c          287 g          478 t
ORIGIN      
        1 gagcacggaa accgaggaat gggttcattt cttttggaag gtcaaagtaa ggaagttcct
       61 tatctccacc aatatccatt gtacgaacca caacaggttt gccattcatg ccttcaagca
      121 ctgccttgta agcttcgtat tgttcgtctt cagttgggaa gtcttgagaa tccatgtaca
      181 agaactcagt acggtaaagg ccaacagctt cagcaccatt gtcattaaca ccttcaacgt
      241 ctttaggcgt accgatatta gcagccaatt caaagtgttt gccatcagct gtttcagtgt
      301 gcgcatcttt aaggagagac cactctgctt tttgtttggc ataagccgca ccagcttctt
      361 taaaagcaag tacttgatct tcgcttggat cgataatcac ttcaccagtg ataccattaa
      421 cggcaatcac atcaccatct ttaacacgtt ttgtaatatc atttgttcca agtaccgcag
      481 cgatctcaag tgtacgtgcc atgatagctg agtgacttgt acgaccaccg atatttgtaa
      541 caaatgcttt tacaaattgt ttgttaagtt gagcagtatc tgaaggtgtc aaatcgtgtg
      601 cgataacgat tgattcttca ttgattgtag ctggatttgg aagttttaca cctaaaaggt
      661 gagccaacac acgttttgca acgtcgcgga tgtccgctgc acgttcttgc atgtatgggt
      721 tatcttccat gccttcaaag atggtgatga acatgtcagt cacttcttta agacctgttt
      781 ctgcattcgt ttgttttgcg cgaatcgttt ctttaacctg gctgatcatt tctggatcag
      841 caagaaccat caaatgggca tcaaaaacgg ctgctgcttc ttcacctaag ctttctactg
      901 cattttcacg gataacagaa agctcgtctt gtgcagcttg gagtgcaaca tcaaggcgag
      961 cttcttctgc atttgtatct gcgactgtaa cagtctcaaa tgacaaatcc ggctgaacta
     1021 gtagatatgc tttagcaaca gcaacgccgt ctgaggctgc aattccttta agcatttctg
     1081 tcatagtctt atgccaatcc ctctttagtc attgtttctt caattgctgc aatggcatct
     1141 tcagcatctg caccttcagc tgagatagta acatctgcac cttgaccaac accaagactc
     1201 ataacaccca tgattgattt tagatttact gctttacctt tgtagtcaag agtgatgtct
     1261 gaagcaaatt tgctagctgt ttgaacaagc aaagtcgctg gacgcgcatg aatacctgtt
     1321 tctgcaacaa tgtgaaagtc ttttgaagcc atagtatggt tctcctttta agttgtgtat
     1381 ttttgagcca tcacatgata acccttacaa caaccgatta tagcacaatg cttttttatt
     1441 ttcaagatta aatatttaaa cgacaaaaaa tgtgaaatta ttttctttta ttttcacg
//
LOCUS       DAUVUY010000167         1476 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.168, whole genome shotgun sequence.
ACCESSION   DAUVUY010000167 DAUVUY010000000
VERSION     DAUVUY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.168"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..737)
                     /gene="dnaN"
                     /locus_tag="VOX58_001956"
     CDS             complement(<1..737)
                     /gene="dnaN"
                     /locus_tag="VOX58_001956"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HES5879144.1"
                     /translation="MIQFSINRTLFIHALNATKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQH
                     QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
                     ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDD
                     IETVEVFFSPSQILFRSEHISFYTRLL"
     gene            complement(892..>1476)
                     /locus_tag="VOX58_001957"
     CDS             complement(892..>1476)
                     /locus_tag="VOX58_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HES5879145.1"
                     /translation="NDLEDRLVTRFKWGLTVNITPPDFETRVAILTNKIQEYNFIFPQ
                     DTIEYLAGQFDSNVRDLEGALKDISLVANFKQIDTITVDIAAEAIRARKQDGPKMTVI
                     PIEEIQAQVGKFYGVTVKEIKATKRTQNIVLARQVAMFLAREMTDNSLPKIGKEFGGR
                     DHSTVLHAYNKIKNMISQDESLRIEIETIKNKIK"
BASE COUNT          472 a          234 c          244 g          526 t
ORIGIN      
        1 tctaagaggc gtgtataaaa agaaatgtgt tcacttctga acaagatttg gcttggtgag
       61 aaaaatacct caacggtctc aatatcatct gtaaatactg ctgaaaattc tctcaaagat
      121 ttacttggaa taaccacatc aaaatctgct gaagtattgt ccaaagtgat taaacgttgg
      181 ctcatacgat gagagtcagt cgctactgct ttaaaatctt tatgattgct taatacaata
      241 tgaactcctg ttaaaatagg acgactttct tgtaaactgg ctgcaaaagc tgtttcagca
      301 ataatagact tcaataattt tgtttttaaa atcaaaggat tttctgttga tacttcttgt
      361 agacgaggat actggtcaac atcttttcct tttaaggtaa tctctgattt accactggtt
      421 aaaacaactt ggtgttgttc aatttcttta acatttatac taatatctgg caaacttgaa
      481 ataatattaa taaaaaaact agcttctaat aaaatagctc ctggagaggt aattagcaaa
      541 ccagcatttt cattacttac aggaatagtg ttttcaattg atatttgacc gttagaccct
      601 gttaaagtta ctcctgtaga agtgacttca atttttattg atgaaagaat aggaatggca
      661 tttttagtgc taatagcacg tttagttgca tttaaagcat gaataaataa tgtgcgatta
      721 attgaaaatt gaatcatgag aactccttta tttaataagt ataataataa gtagtagtag
      781 taggtcttgt gtatatagtg gataactcta aataaacgag agaatccaag actaaatggt
      841 tgttcacaac ttgtggataa ctatttcata aaagatattc ttttccacat gttatttaat
      901 tttgtttttt atggtttcaa tttcgatcct aaggctttcg tcctggctga tcatgttttt
      961 gattttatta taggcatgga gtactgttga atggtctctg ccaccaaatt cttttccaat
     1021 tttaggaaga ctgttatctg tcatttcacg tgctaaaaac atagctactt gtcttgctaa
     1081 aacaatattt tgtgttcgtt tagtagcttt aatttctttg acggtaacac cgtaaaattt
     1141 tccaacttgc gcttgaattt cttcgatggg aataactgtc attttaggtc catcttgctt
     1201 tctggcgcga atagcttcgg cagcaatgtc aacagtaatc gtgtcaattt gtttgaaatt
     1261 agcaaccaga ctaatatctt ttaaggcacc ttctaaatct ctgacattag aatcaaattg
     1321 accagccaaa tactcaatgg tatcttgagg aaaaataaag ttatattctt gaattttatt
     1381 tgtcaaaata gccactcgtg tttcaaaatc aggaggtgtg atattgactg ttaatcccca
     1441 tttaaaacga gtaactaatc gatcttctaa atcatt
//
LOCUS       DAUVUY010000168         1389 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.169, whole genome shotgun sequence.
ACCESSION   DAUVUY010000168 DAUVUY010000000
VERSION     DAUVUY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.169"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..345
                     /locus_tag="VOX58_001958"
     CDS             <1..345
                     /locus_tag="VOX58_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycerophosphate oxidase"
                     /protein_id="HES5879146.1"
                     /translation="DARYLANLYGSNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYE
                     MALKPTDYFLRRTNHLLFMRDSLDALIDPVINEMAKHFEWSDQERVAQEDDLRRVIAD
                     NDLSALKGQQEG"
     gene            347..1048
                     /locus_tag="VOX58_001959"
     CDS             347..1048
                     /locus_tag="VOX58_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HES5879147.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            1157..>1389
                     /locus_tag="VOX58_001960"
     CDS             1157..>1389
                     /locus_tag="VOX58_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HES5879148.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESN"
BASE COUNT          366 a          279 c          316 g          428 t
ORIGIN      
        1 gatgctaggt atttggcaaa cctttatggt tctaatgcgc caaaagtctt tgccttaact
       61 cgtcaattaa cagcagctga agggttaagt ttagctgaaa ccttgtcctt acattatgcg
      121 atggattatg aaatggctct taaaccgaca gattatttct tgagaagaac aaatcacctc
      181 ttatttatgc gagatagcct agatgctttg attgacccag tgattaatga aatggctaaa
      241 cattttgaat ggtctgatca ggaaagagtg gcacaagaag acgatcttcg tcgcgtgatt
      301 gcagacaatg atttgagtgc cctaaaaggc cagcaggagg gttaatatgg atatctttgg
      361 tgaatttttg ggtacagcct tattggtttt actaggaaat ggtgtggtag caggggttgt
      421 cttacccaaa acgaaaaccc atgcttctgg ctggatcgtg attgcgactg gctggggaat
      481 tgcagttgct gtggcagttt ttatcagtgg caaagttgcc cctgcccacc tcaatccagc
      541 tgttagcctt gcttttgcca tgagtggaac cattgcttgg tcaacagcta ttgcctatag
      601 ccttgcccaa ctattgggag ctatggttgg ttcaactctg gtattccttc agttcaggcc
      661 acattatctg gctgctgaga gtcaggctga tattttaggg acatttgcga caggtcctgc
      721 tattcgagat accagctcaa acctattgag tgaaatcttt gggacctttg tcttgatgct
      781 tggtattttg gcatttggct tatatgatat gccagcagga ctaggaaccc tctgtgtagg
      841 taccttggtt attgggattg ggttatcctt aggaggaacg acaggttacg ccattaaccc
      901 agcgcgtgat ttaggacctc gtctagttca tgctattcta ccactcaaca acaagggaga
      961 ttctgattgg tcttatgctt ggatacctgt tgtaggacca attattggag cggccttagc
     1021 agttttgctt ttccaagtca tgtcctaacc atcactaaag gggttggcta tcataggcca
     1081 gctcctttgc tttttcttta taggattagg taaaacgctt tcatatctta aaaacaactg
     1141 ctataataat actattatga cattaacgac aaagaccata catgttatcg gggcatcctt
     1201 tgctggtcta gcctttgttg ataaatataa ggacctcaat ccagattccc aaatcattct
     1261 cattgataag gaaagctgtc ccaattatat tccaaatggt atcaaccaat tattcagagg
     1321 agacattcaa gacctgtctg atgctatgtg gggaagggcc tgcctagcag cacaaataga
     1381 atctaacca
//
LOCUS       DAUVUY010000169         1344 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.170, whole genome shotgun sequence.
ACCESSION   DAUVUY010000169 DAUVUY010000000
VERSION     DAUVUY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.170"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..579
                     /locus_tag="VOX58_001961"
     CDS             <1..579
                     /locus_tag="VOX58_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HES5879149.1"
                     /translation="QYRFDGFEGEWEEKKLGEISRMFSGGTPNVGIPEYYNGNIPFIR
                     SAEINSDQTELSITDKGLSNSSAKLVEKNTLLYALYGATSGEVGLSRISGAINQAILA
                     IIPEKKYSSLFIKNWLYKQKSSIIEKYLQGGQGNLSGSIVKELTIHFPSLSEQEAIGD
                     FFQTLDQQMSQTEDKLIELKALKQTLLNRLFV"
     gene            592..>1344
                     /locus_tag="VOX58_001962"
     CDS             592..>1344
                     /locus_tag="VOX58_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HES5879150.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLIAVCDNLEEHFNTFTDAQKIFEDAYQDEELKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFESVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGREDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQ"
BASE COUNT          464 a          242 c          232 g          406 t
ORIGIN      
        1 caatatcgct ttgacggttt tgaaggggag tgggaagaga agaaattggg ggagatatct
       61 cggatgtttt caggtggcac tcctaacgta ggtatccctg aatactataa tggtaatatt
      121 cctttcatta gatctgctga gatcaacagt gatcaaacag aattatctat cacagacaaa
      181 ggtctatcaa attcatcagc aaaattagtg gaaaaaaata cattattata cgctctctac
      241 ggtgcaacta gtggagaggt tggactttca agaataagtg gagctattaa tcaagcaatt
      301 ttagccatta ttcctgagaa aaaatattct tctcttttta taaaaaattg gttatataaa
      361 caaaaatcct caattattga gaaatattta caaggtggcc aaggaaattt atctggttct
      421 attgttaaag aattaactat tcatttccct tctctctccg aacaagaagc tatcggtgac
      481 ttcttccaaa ccttagacca acaaatgagt caaacagaag ataaattaat agaacttaaa
      541 gcactcaaac aaacattact caatcgttta ttcgtataag aaaggactca catggcagag
      601 aaaacaactt cactccggca agcactatgg cattcggccg accaattacg tggtcaaatg
      661 gatgccaatg actataaaaa ctatcttctt ggactcatct tttacaagca cttatctgat
      721 aaattactca tagctgtttg tgataattta gaagaacact ttaatacttt tacagatgct
      781 caaaaaatct ttgaagatgc ctatcaagat gaagaactaa aagatgacct catttctgtt
      841 gttactggtg atttaggtta ctttatcgaa ccgacactga cctttgaaaa actcatccaa
      901 gatgtttatc acaatacttt ccaattagaa tcattagctc aaggttttcg cgatattgaa
      961 caaagtggcg aagactttga aaacttattt gaagatattg acctctactc taaaaaatta
     1021 ggctctactc ctcaaaaaca aaatcaaacc atttctaatg tcatgaaaac attaaatgaa
     1081 attgactttg agtcagtaga tggggatact ttaggtgatg cttatgagta cttgattggt
     1141 gaatttgcca gtgagtcagg aaaaaaagca ggtgagttct ataccccaca agctgtctct
     1201 cacttaatga ctcagattgt ctttttaggt cgtgaagatc aaaaaggaat gaccctctat
     1261 gaccctgcta tgggatctgg atcactcctt ttaaatgcta agaaatatag taaccaatcc
     1321 gataccgttt cttactatgg tcaa
//
LOCUS       DAUVUY010000170         1293 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.171, whole genome shotgun sequence.
ACCESSION   DAUVUY010000170 DAUVUY010000000
VERSION     DAUVUY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.171"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..491
                     /locus_tag="VOX58_001963"
     CDS             <1..491
                     /locus_tag="VOX58_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HES5879151.1"
                     /translation="SISVENVLERAGDSTPFSIALESIDAMKTIEEITIAGSGKASFS
                     PLTFTTVGQYTYRVYQKPSQNKDYQADTTVFDVLVYVTYDEDETLAAKVISRRAGDEE
                     KSAITFKPKRLVKPIPPRQPDIPKTPLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKS
                     RL"
     gene            complement(650..>1293)
                     /locus_tag="VOX58_001964"
     CDS             complement(650..>1293)
                     /locus_tag="VOX58_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HES5879152.1"
                     /translation="NHIMDLVKLGNPQLLKQEINRIPLSSITSSSISALRAEKNLTVI
                     YLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRCAAIIHFSESLTNKS
                     ISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFKKYSNVSLQHYILST
                     KIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKDYLAKYRDNI"
BASE COUNT          416 a          207 c          244 g          426 t
ORIGIN      
        1 ctagcattag tgtcgaaaat gtcttagaga gagcaggcga tagtacccca ttttcgattg
       61 cattagaatc aattgatgcg atgaaaacaa tagaagaaat aacaattgct ggttctggaa
      121 aagcaagctt ttcccctctg accttcacaa cagttgggca atatacttat cgtgtttatc
      181 agaagccttc acaaaataaa gattatcaag cagatactac tgtatttgac gttcttgtct
      241 atgtgaccta tgatgaagat gagactctag ccgcaaaagt tatttctcga agggctggag
      301 acgaagaaaa atcagcgatt acttttaagc ccaaacggtt agtaaaacca ataccgccta
      361 gacaacctga catccctaaa accccattac cattagctgg tgaagtaaaa agtttattgg
      421 gtatcttaag tatcgtatta ctgggattac tagttcttct ttatgttaaa aaactgaaga
      481 gtaggctata aaacccattt ttaagagtca cacattttta gttgctctat ttttataatg
      541 aaacttattg acatctccat agtcttgctt gactttggag atgttttcta tgctgatagg
      601 tttttgtacc atataagata atgattgatg aaaagagtgt cttgaattct taaatattat
      661 ctcggtattt agcaagatag tcttttgaag aaatacccgt gtatttttta aagattttat
      721 gaaaatgggt agtgtcataa aaatataagc ttttagccac ttctccaaca ggaattcctc
      781 gttttaagag tagttgagct tctttgattt ttgtacttag aatataatgt tgtaaggaaa
      841 catttgagta ttttttaaag actgaacgta agtgagattc ggaaacatat aggcgcttag
      901 cgatatcaga tacctttaat ttggaataca ggtgactatc gacataatga agcacactat
      961 tgtacatttg acgtttatca gaaatacttt tattggttaa tgattcggaa aaatggatga
     1021 tagctgcaca gcgaattctc aaaactttga gcaagtcact cgcttcttca tttaaggcca
     1081 tgtagtattt gacaaggcta taatgctttg ctacgtcagt attttctaca aaactgaatt
     1141 ccagtaacct agttaaatag ataacagtga ggttcttttc cgcccttaga gcagaaatag
     1201 aagatgaggt gatacttgat aaggggatgc gattgatttc ttgcttgagc aattgtggat
     1261 tgcctagctt taccagatcc ataatatgat tta
//
LOCUS       DAUVUY010000171         1281 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.172, whole genome shotgun sequence.
ACCESSION   DAUVUY010000171 DAUVUY010000000
VERSION     DAUVUY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.172"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            510..932
                     /locus_tag="VOX58_001965"
     CDS             510..932
                     /locus_tag="VOX58_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HES5879153.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            929..1159
                     /locus_tag="VOX58_001966"
     CDS             929..1159
                     /locus_tag="VOX58_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HES5879154.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
BASE COUNT          429 a          216 c          235 g          401 t
ORIGIN      
        1 ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag tttatgagtt
       61 cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata gaaaggtcgc
      121 tgattttatt tacctataca actttataaa atttagtcat actcctagaa agtagataaa
      181 attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca tctagttctc
      241 ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat gaccatcaat
      301 gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga aaacaatctg
      361 tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc atttatgcaa
      421 caaatccccc tttcgtgtcg gggcttagta tgaagggaga taaagaagca catgccactc
      481 cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa gaaaccatag
      541 aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa gattacttca
      601 aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat aactaccttg
      661 tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt aaattatttg
      721 gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat ctttcagaga
      781 aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg gagattgcgg
      841 agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga ctagccatga
      901 ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg ttcccttccc
      961 cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg ttaaacatta
     1021 tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg gaaattctca
     1081 tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct ctaagatttt
     1141 atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg tatccacgct
     1201 tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt aggttttgat
     1261 aggctaacaa gccaaacgtt c
//
LOCUS       DAUVUY010000172         1182 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.173, whole genome shotgun sequence.
ACCESSION   DAUVUY010000172 DAUVUY010000000
VERSION     DAUVUY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.173"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>1182
                     /locus_tag="VOX58_001967"
     CDS             <1..>1182
                     /locus_tag="VOX58_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HES5879155.1"
                     /translation="SSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAES
                     YFNLSNLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRR
                     FSDGLHQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYN
                     MRIIPIPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDL
                     ISRKLVEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVREL
                     GGLCVIGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDR
                     MKLDEEDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDV
                     I"
BASE COUNT          353 a          209 c          275 g          345 t
ORIGIN      
        1 ttagctctga aaccaatcaa ttgtatattc gtgctgacat gtttgtaaaa actctgacgt
       61 cagtggatta tgttattgat gtgcccacta aaacgattgg attaagcgat tcaggtattg
      121 ataaagctga gagttatttt aacttgagca atctttacga tattgaaaat gtagccttga
      181 ctcactttat agacaacgcc cttcgtgcaa actatattat gttgttagat atcgattatg
      241 tggtcagcga ggatggtgag attcttattg ttgaccaatt tacaggtcgt acgatggaag
      301 ggcgtcgctt ctcagacggc ttgcaccaag ctattgaggc aaaagaaggc gttcgtattc
      361 aggaagaatc aaaaaccagc gcctcaatca cctaccaaaa tatgtttcgt atgtacaaaa
      421 aattggcagg tatgactggt actgcgaaga ccgaggaaga agaatttcgc gaagtttata
      481 acatgcgtat tattccaatt ccaaccaatc gtccaattgc ccgtattgat cacactgatt
      541 tgctttaccc gaccttagaa tcaaagttta gagcagttgt ggaagacgtt aaaacgcgtc
      601 atgcaaaagg acaacctatc cttgtaggta cggttgctgt tgaaactagt gatttgattt
      661 cacgcaagct ggttgaagca gggattcctc atgaggtttt gaatgctaaa aatcacttta
      721 aagaagctca aatcattatg aatgctgggc aacgtggtgc tgttacaatc gcaactaaca
      781 tggctggtcg tggtactgat atcaagcttg gtgaaggtgt tcgtgaactt ggtgggttat
      841 gtgtcattgg tacagagcgt catgaaagcc gtcgtatcga taaccagctt cgtggacgtt
      901 caggtcgtca aggagatcca ggtgaatccc aattctatct ttcattagaa gatgatttga
      961 tgagacgttt cggctctgat cgtataaaag cctttttgga tcggatgaaa ttagatgaag
     1021 aagacactgt tattaaatca ggtatgttag ggcgtcaagt ggaatctgct caaaaacggg
     1081 ttgaaggaaa taactacgat acccgtaaac aggtcttaca atatgatgat gtgatgcgtg
     1141 aacagcgtga aattatctat gctaaccgtc gtgatgtcat aa
//
LOCUS       DAUVUY010000173         1133 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.174, whole genome shotgun sequence.
ACCESSION   DAUVUY010000173 DAUVUY010000000
VERSION     DAUVUY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.174"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..203)
                     /gene="gatC"
                     /locus_tag="VOX58_001968"
     CDS             complement(<1..203)
                     /gene="gatC"
                     /locus_tag="VOX58_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HES5879156.1"
                     /translation="MKISEEEVRHVAKLSKLSFLESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDIA"
     gene            complement(435..753)
                     /locus_tag="VOX58_001969"
                     /pseudo
     CDS             complement(435..753)
                     /locus_tag="VOX58_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
BASE COUNT          357 a          255 c          202 g          319 t
ORIGIN      
        1 tcagcaatat cttgacgcat cacatttttc ttatctgcca tggttgttgt aatggcgaca
       61 ccttcggtgt caacttcgtt cagcaattca accatatcaa cgattttgga taaggtcgtt
      121 gcaaaggttg ttgtctcact ttctaagaat gataattttg atagcttcgc gacatggcga
      181 acttcttctt cagaaatttt catgatttct cccatccaag atgatcagaa gtattccaat
      241 tccaaagaac agctctgcta ttctccaaag gtagcggaaa aactcctcac atcgttttta
      301 catagtctca attattatat cacaattgcg cccatttacc ataggtgact aatgcaattc
      361 tagaaagcta tacagcaaaa aacttcctga ctaaggtcag aaaaccttag tcaggaagtg
      421 tgacaaacta aactctattt gtcttgggta agactgtggc ctgaagcttt gatggcagcc
      481 tgagcagccg ccaagcgagt aagcggcact ctaaatggcg aacaagaaac gtaggtcagg
      541 ccttggctgt ggtaaaagtc aatggaagca ggatcgccac catgttcccc acaaatacca
      601 agtttcaact caggcttaac cgaacgggct aaagagacgg ccaatccctt tctgatcaaa
      661 ggtctggaac ggctccttat caaaaatacc cctgtcaacg tattcttcca agtattttcc
      721 tacaacatca cagaaaaagt aacgttcttt caagtcggta atatgtttac gaaggtagta
      781 aaggggtgct ttattctaat ttaccaaatc ctcaacgatt gtgacaacat gtgatgtgat
      841 aagcctaaga taaaaatggt acagactcta gcattcaaga gtttgaagct ttatttatat
      901 tctaatgctg aaaagactag agtttgtcta atggctgtga gacgatcctc taaaccacca
      961 actcaaatca tttctcaaac agctaaaatc ctgtctcctt caaaaaaaca tgtgttctat
     1021 ctgacacagt ccaaagttta gttgcctttt agacagcaca agaatcatca ggactaattc
     1081 tgaccagcat gtcacatacc tcatctacct aacccataca tacggtaaaa cca
//
LOCUS       DAUVUY010000174         1095 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.175, whole genome shotgun sequence.
ACCESSION   DAUVUY010000174 DAUVUY010000000
VERSION     DAUVUY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.175"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(1..284)
                     /locus_tag="VOX58_001970"
                     /pseudo
     CDS             complement(1..284)
                     /locus_tag="VOX58_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(481..>1095)
                     /locus_tag="VOX58_001971"
     CDS             complement(481..>1095)
                     /locus_tag="VOX58_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HES5879157.1"
                     /translation="FRQKVSFAESTTAFSKEFFGNLLVLAITKWLFFLIWSLIWFFGL
                     FIFLSGLSAFLVNAKSGGSTVISLIFLLFGAVLSLIGFGIYINRYYAYSLSEYLLYDK
                     VKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLSFTGWFLLNIVTFGLLNIYLLPYFTT
                     ANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKKPTPMMYDDQKD"
BASE COUNT          354 a          194 c          190 g          357 t
ORIGIN      
        1 ttccttctga agacgttcaa cctctgtcat ctcttccaaa tttttcttac gtttacgccc
       61 catcttggtt tttcaagaat agtataaccg tttttcttgt attgcgctat ccaccttgag
      121 agcatactag aagttagtaa agcataatct aaggatgttt gtttctgaga ttggccgtca
      181 atcaggactt tatttattat ctcttgcttc agttctggag aataataatt attcttacat
      241 ttttgaacaa tggttacccc atacctatca atcaagcgaa tcatatattt gagatcggat
      301 tctgccatat caaacttttt tgatatggat ttaatggact ctcccatttg ccgtaactca
      361 taaatcttaa ctttcgtttc ttgattaaat ttcataaaaa aatagcaccc caatcgttag
      421 attttgtgtc taacttttgg ggtgcagttc aagggctatt tttatggatg gaggatttat
      481 ttagtctttt tgatcgtcat acatcatagg agtaggcttt ttattaagct gatgaaggct
      541 taaatgttct ccttcaattg ggtcatcttt gtctttgaaa cgtttcttga gttgatcata
      601 aaaaatgaca ttggctgttg taaaatatgg taaaagatag atattaagta acccaaaagt
      661 aacaatattc agtagaaacc aaccggtgaa gctaagttgt aagaaaaaaa gcttccattt
      721 ataacctttc atcatagcta cacttgtttc aatgacggct atagctccta gataagtccc
      781 ctctttaacc ttatcgtaga gaaggtattc tgatagacta taagcgtaat aacggttgat
      841 ataaatacca aaaccaatta aggacaaaac tgctccaaat agtaaaaaga tcagtgaaat
      901 gactgttgag ccacctgatt tagcattgac caaaaacgct gaaagtccgg aaaggaaaat
      961 aaagaggcca aaaaaccaaa ttagtgacca gatgagaaag aatagccact tagtgatcgc
     1021 taaaaccagc aggttcccaa agaattcttt tgagaaggct gttgttgatt cggcaaaaga
     1081 aactttctga cgaaa
//
LOCUS       DAUVUY010000175         1092 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.176, whole genome shotgun sequence.
ACCESSION   DAUVUY010000175 DAUVUY010000000
VERSION     DAUVUY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.176"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..1044)
                     /locus_tag="VOX58_001972"
     CDS             complement(<1..1044)
                     /locus_tag="VOX58_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HES5879158.1"
                     /translation="MFPIAFWDVIHSFIVYFIVFNISFLKNNYHSNIIIMIFSIVAGT
                     MVLLLIQAFNYDFSHLRKKKLNQRTRTIISIANFSMILYFITIPMIDFIEEVENINFT
                     HYQEMITAIYFLLFICFVNLLDRNLRQELQKQVVLQKELQLQNMARYSKQIEGLYQNI
                     RSFRHDYANILTSLKIGIDCKDIEMISSIYHKVLKDSGKVLRGEKFNLARLRNINDLP
                     LKSLLFVKLSEAQRLSIPVSIEIGEPISIKNIELIDFLTLVSILIDNAIEGAAESGIV
                     LCFFEQFGKQILIVQNDTFEKQINLSTIFERGVSSKGNNRGVGLSNVREILESYPTVT
                     LKTVSNHFVFKQIL"
BASE COUNT          403 a          177 c          142 g          370 t
ORIGIN      
        1 tagaatctgt ttaaaaacaa aatgattact tactgttttg agggtaacag tgggatatga
       61 ttctaatatc tccctaacat ttgataaacc aactccccta ttatttcctt ttgaagaaac
      121 acctcgctca aaaatcgtgg aaagattaat ctgtttttca aaagtatcat tttgaacaat
      181 taatatctgt ttaccaaatt gttcaaaaaa acaaagaact attcccgact ctgctgcacc
      241 ttcgattgca ttatctatca agatagaaac aagggttaaa aaatcgatta actcaatgtt
      301 ttttatagaa atcggttccc caatttctat agatacaggt atggataagc gttgagcttc
      361 tgataattta acaaacagta aacttttcag aggaaggtcg tttatatttc tcagccttgc
      421 tagattgaat ttttctcccc ttaaaacctt ccctgaatct ttcaacacct tatgataaat
      481 gcttgaaatc atttcaatat ctttgcaatc aatgcctatt ttcaagcttg ttaaaatatt
      541 tgcataatca tgtctaaaac ttctgatatt ttgatacagt ccttcgattt gcttactata
      601 gcgagccata ttctgtaatt gtaactcttt ttgtagaaca acttgctttt gtaattcttg
      661 gcgtaagttc cgatccaata aatttacaaa acaaataaat aaaagaaaat aaattgctgt
      721 tatcatctct tgatagtgcg taaaattaat attttcaact tcttcgataa aatctatcat
      781 tggaatggta ataaagtaca atatcatgct aaagttggca atagatataa tcgttctagt
      841 acgttgattt agtttctttt ttcttaaatg actaaagtca taattaaaag cttgaatcag
      901 cagcaaaacc atagtaccag caacaataga aaaaatcata ataatgatat ttgaatggta
      961 gttatttttt agaaaagaaa tattaaaaac aataaagtaa acaataaaac tatgaattac
     1021 atcccaaaaa gcaataggaa acaaactata aaataaaaga agtgaaccat tgacctttcg
     1081 tcttgcaata ta
//
LOCUS       DAUVUY010000176         1078 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.177, whole genome shotgun sequence.
ACCESSION   DAUVUY010000176 DAUVUY010000000
VERSION     DAUVUY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.177"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            211..825
                     /locus_tag="VOX58_001973"
     CDS             211..825
                     /locus_tag="VOX58_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HES5879159.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQAYI"
BASE COUNT          359 a          154 c          194 g          371 t
ORIGIN      
        1 gtcgttaatg aagctgatac aggttttgtc cgtgaggtat tgatgtaggt attaatttat
       61 cacaattcgg aaagttcgag cttgtctttt agactaagtc ttcgaacgtt ttactgctag
      121 agtttcatct gaaccaaagt gaaacgcttt tgatgaagtc aagtaggctt tttgttatac
      181 taacaataaa gataagctaa ggaggctgag atgttagaaa ttgtttcaga agagctacgt
      241 cactatcaag aacaaaaatt gattgagtac cgtaacaaaa atcagttagc cccaaaagga
      301 ggcattgtat ttgcaggtga ttctctcatt gaattttttc ctttaaaaaa agcttttggt
      361 tcatgtttgc ctattattaa tcgaggtatt gctgggatag atagtcagtg gttattgcgc
      421 catttttctg tccaaattac tgatttagag cctaaacata tttttttatt aattggttgt
      481 aatgatatcg ggcttggata tgataaatgt catattgtca agactattgt agagcttatt
      541 agccagattc gtagtcattg tgtctacagt cagatttatc tcttatcttt attaccagtt
      601 tccaacaatc cccgatatca gaagactgtc aaaataagaa caaatgctat gattgatgct
      661 attaataaag atttagcaat gattccaact atagaattta ttaatcttaa tacttgtctt
      721 aaagacgaaa aaggtggttt atcagatgag aatactctag atggtttaca tcttaatttt
      781 ccagcttatg ctaaactagc tgaaattatt caagcttata tctagcaaat aaatgtacta
      841 atagcgatga ggcaagaagt cagatagcag atggttcatc aatcaattaa gaaaaaagtt
      901 atcaagatac cattaaaaat agttgctcaa agttctttca aatcaaaaga ttatgatatc
      961 gctatgttga gttttagtgt ataatgataa ttaaaggagt taccatgaaa tttgaaaaaa
     1021 aacaagtctt ctatttagtc cttacgttta tcttatgcta tgggatccta gcaaactg
//
LOCUS       DAUVUY010000177         1061 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.178, whole genome shotgun sequence.
ACCESSION   DAUVUY010000177 DAUVUY010000000
VERSION     DAUVUY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.178"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            31..432
                     /locus_tag="VOX58_001974"
                     /pseudo
     CDS             31..432
                     /locus_tag="VOX58_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
     gene            complement(490..936)
                     /locus_tag="VOX58_001975"
     CDS             complement(490..936)
                     /locus_tag="VOX58_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HES5879160.1"
                     /translation="MSYKYCFGRDALSDISLTIKEGEKVSIVGISGSGKTTLAKMIVN
                     FFEPYEGQISINHQNLKMIDKKVLRQHINYLPQQAHIFSGSILENLTLGANHSTSQED
                     ILINIRNDIHILKFDTYFQTGQNIKKEKRIKRKIFHSYFIYIYFSM"
BASE COUNT          342 a          176 c          171 g          372 t
ORIGIN      
        1 agacttgaat aactatcata attttttcca cgtattgaac aatcaattga ctatgtccat
       61 catcaacaag gtcaaacgtt agctgaagac tcctttattt ttaaagatgc caagactcag
      121 gttcagattc cattcagatt ccatttgcag acttacttta tattgaaacg tcatatattg
      181 ctcataaatt ggttctttat tctactagag aaaggattga attttatggg gagttatctg
      241 aaatcgttaa gcaagaacca agattatttc aatgccatcg atcatttgtt gttaatccct
      301 acaatatatc ttcaatacaa cgattagaac gtaaggttta tttaaaaaaa cggttcatct
      361 tgtctagtat caaggctaaa agttactgct ctaaaagaag cagttgaaaa tctacataat
      421 aaagagactt agatgagtag catcttcttt ctagcactta cttttactca tgtcattatt
      481 ttgataattt tacatgctaa agtatatgta tataaagtaa ctgtgaaaaa tctttcgctt
      541 tatacgcttt tcttttttta tattctgccc agtttggaaa taggtatcga atttcaagat
      601 atgaatatcg tttctgatat ttatcagaat gtcctcttga ctagtggagt gattggcacc
      661 tagggttaag ttttctaaga tagatccact aaagatatga gcttgttggg ggagatagtt
      721 gatatgctga cgaagcactt ttttatcaat cattttaaga ttttgatgat taatagaaat
      781 ctgtccctcg taaggttcaa agaaattaac aatcatttta gctaatgttg tcttgccaga
      841 accactaata ccaactatac tgactttttc accctcctta atggtcaaac tgatatcaga
      901 taaggcatca cgaccaaagc agtatttata agacaagtct gcaaattgga tatctccata
      961 tagacaatag gtatctgtta atgcctgttc tttttggaac tcagatttaa ccaggtaaac
     1021 ttcattgaga cgattattag ctaccttagc ggattgcagt t
//
LOCUS       DAUVUY010000178         1043 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.179, whole genome shotgun sequence.
ACCESSION   DAUVUY010000178 DAUVUY010000000
VERSION     DAUVUY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.179"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..128)
                     /locus_tag="VOX58_001976"
     CDS             complement(<1..128)
                     /locus_tag="VOX58_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HES5879161.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQA"
     gene            488..>1043
                     /locus_tag="VOX58_001977"
     CDS             488..>1043
                     /locus_tag="VOX58_001977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879162.1"
                     /translation="MKKTLTLLLALFAIGVTSSVRAEDITFKQVPGQQTIPGITVKSF
                     KVGDSTISVQLGSYEYVYGSIKHRDNSVDKINPRAKEVKVTCPTTSNGCIERKWMVSG
                     NRADGSGYWSVQLTKELQEGDIVTLSFADDGNLIFGKLVFEDEKTQNTRLQAEKAQEQ
                     EEEKKRDNELRKQLEESMLKQIREE"
BASE COUNT          376 a          200 c          204 g          263 t
ORIGIN      
        1 gcctgtagta ctttcccatc cacttcaagg gtatcaaata actgcacata attttcagcg
       61 ccaccccaac ctgtacgtac atattttttc ttggctttta aacctggtag aatttcttca
      121 aatgtcatat tgttctcctt tattgacgaa atggacactt taggcagtct ataaactcct
      181 aaaaataaat ttgtggactc acgcatcatt caccgaaaat gctatcttct tttatttagt
      241 ctatcataac gcatttttgt caaaaagcaa ctaaaagtat tggcaacctc aaaacacgac
      301 ctgatgccca aacggcttgc aaaggtaaaa gggcaacggc tagcaactgg ctagaaaaaa
      361 actgtctgcc cctaccaaaa atagtcgcgc catttttcaa ccacaagaca taaacgccct
      421 atctttttag cgcaagaatt ggtataataa gccttgtctt aaaaaatgag caaaaggaga
      481 ggaaaaaatg aaaaagacat taaccttgct actggcactc tttgccatcg gggtaactag
      541 tagcgtcaga gcggaggata tcaccttcaa gcaagtcccc ggacaacaaa ctataccagg
      601 gatcacagtt aaatctttta aagttggaga ttcgactatt agcgtacagc taggttctta
      661 tgaatatgta tatggtagta taaagcatag agataatagt gtagataaaa taaatcctcg
      721 agctaaggaa gttaaggtta cgtgtcctac aactagtaac ggctgtatag aacgaaaatg
      781 gatggttagt ggtaatcgcg ctgatggtag tgggtattgg agtgttcaac tcactaaaga
      841 attacaagaa ggagacattg tgactctctc cttcgcagac gatggcaact taatatttgg
      901 taaactagtc ttcgaagatg aaaaaacaca aaacacaagg ctgcaagcgg aaaaagcaca
      961 ggaacaagaa gaggaaaaaa aacgcgataa tgaattaaga aaacaactag aagaaagcat
     1021 gctaaagcag ataagagaag aag
//
LOCUS       DAUVUY010000179         1042 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.180, whole genome shotgun sequence.
ACCESSION   DAUVUY010000179 DAUVUY010000000
VERSION     DAUVUY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.180"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..372
                     /gene="lepB"
                     /locus_tag="VOX58_001978"
     CDS             <1..372
                     /gene="lepB"
                     /locus_tag="VOX58_001978"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HES5879163.1"
                     /translation="KRVIGLPGDTISYNDDTLYINGKKTVEPYLAEYLKQFKNDKLQK
                     TYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGDDRIVSRDSREVGSFKK
                     ENLIGEVKARFWPLNKMTVFN"
     gene            430..>1042
                     /locus_tag="VOX58_001979"
     CDS             430..>1042
                     /locus_tag="VOX58_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HES5879164.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMADALA"
BASE COUNT          342 a          192 c          200 g          308 t
ORIGIN      
        1 aaaagagtta ttggattgcc aggcgatacc atttcttata atgatgacac actttatatt
       61 aatggtaaaa aaacagttga gccgtatttg gctgagtatc taaaacaatt taaaaacgat
      121 aaactccaaa aaacttacgc ctataatacc ctattccaac agttagcaga aacatctgat
      181 gcttttacaa ctaattctga gggacaaaca cgctttgaga tgagtgttcc aaaaggagaa
      241 taccttcttc ttggtgatga tcgtattgtt tccagggata gtcgcgaagt tggtagtttc
      301 aaaaaagaaa accttatcgg tgaagtgaaa gctcgttttt ggccactcaa taaaatgacc
      361 gtctttaatt agaaaaaacc tgcctttggg caggttttca gttatcttgt ataaatagaa
      421 agagttacta tggaatacgt ttttacaggt actgttgatc gtatcatttt tgaaaaccag
      481 gctaatttct ttaagattct cctccttgcc attgaggata cagacagtga cattgacgac
      541 tttgaaatca ttatcacagg aacgatggct gacattattg aaggagatga ctacaccttt
      601 tggggagaat tgacccagca ccctaaatat ggacagcaac tcaaactaag ccgttaccaa
      661 aaaatcaaac ctagttcatc tggtttggtt aattatttct ctagcgacca ttttaagggg
      721 attggtaaaa aaacagcgga gaaaatcatt gcactatatg gtcataatac cattgaccat
      781 attttagaag acccaagcaa attagaaact atctctggcc tatctaaggc taatcgtcaa
      841 gcctttgtcg ctaaactaaa attgaattac ggcacagagc aactgattgc tggtctcgtc
      901 gaacttggcc ttagcaaccg ttttgccttt caagcgtttg aaaagtataa agaagaggct
      961 cttgaccttg ttaaagaaaa tccctatcag ttagtcgaag atctacaagg ttttgggttt
     1021 aaaatggcag atgctctcgc tg
//
LOCUS       DAUVUY010000180          946 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.182, whole genome shotgun sequence.
ACCESSION   DAUVUY010000180 DAUVUY010000000
VERSION     DAUVUY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.182"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..536)
                     /gene="tsaD"
                     /locus_tag="VOX58_001980"
     CDS             complement(<1..536)
                     /gene="tsaD"
                     /locus_tag="VOX58_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HES5879165.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLT"
     gene            complement(526..>946)
                     /gene="rimI"
                     /locus_tag="VOX58_001981"
     CDS             complement(526..>946)
                     /gene="rimI"
                     /locus_tag="VOX58_001981"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HES5879166.1"
                     /translation="TVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIRRDQTDYFLLYDH
                     DKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLDSIESDIFLEVRESNHRA
                     QGLYQKFGFKFIGKRPDYYRNPIETALLMKREGKHDR"
BASE COUNT          288 a          227 c          151 g          280 t
ORIGIN      
        1 taggttaagc ccatcacacg cccaacttta tcataagcct caccaacagc gtcgtcacgc
       61 gtctcgccaa taatatgata atcccctggc tctgggacat aaaccaattc tgtgtgtcct
      121 cctgaaacca atagtgctat taaaggataa actaaaggct tttgttcacg cgctgccatt
      181 aagtgacctg ccatatgatt aaccggaatc aaaggtaaat gatttgccca agcaaaagcc
      241 ttcgcagctg ctaatccaac taacaaggct ccaacaagtc cagggccata agttaccgca
      301 actgctgata aatctgaagc actgatgcca gcttcttgca aagcatcctc aaagcaagtc
      361 gtaatcactt cgacatggtg tctactagct acttctggca ccacaccacc aaagcgtttg
      421 tggctctcaa cctgacttgc aatgacatta cttaagaggg tactttcgtt ttttaaaatc
      481 gctacactgg tctcatcaca agaactctca actgctaaaa tatacctatc tgtcatgttt
      541 tccttcccgt ttcatcaata aagctgtttc tataggattt ctatagtagt ctggtctttt
      601 gccaataaat ttaaaaccaa acttctgata taaaccctgt gctctatgat tagattctct
      661 aacctctaaa aagatgtcac tttcaatgct atctaaatgc gtcatcaatt gagacgctag
      721 cccaagtcct tgatgactag gtagtatagc aatttgagtc atttcaactt caccagctaa
      781 atcttgaata gctaaaaaac ctagcaattt atcatgatca tatagtagga aataatctgt
      841 ttgatcccgc cgtatatcga ttaatacttg ttctaaagtc caaggagacg tgccataaac
      901 catttccaaa agctgataaa tattttttgc ttgttcttca actgtt
//
LOCUS       DAUVUY010000181          892 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.183, whole genome shotgun sequence.
ACCESSION   DAUVUY010000181 DAUVUY010000000
VERSION     DAUVUY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.183"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..93
                     /locus_tag="VOX58_001982"
     CDS             <1..93
                     /locus_tag="VOX58_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HES5879167.1"
                     /translation="GETANPFFTAAAATVMVSAGMLALKRKEEN"
     gene            302..>892
                     /locus_tag="VOX58_001983"
     CDS             302..>892
                     /locus_tag="VOX58_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HES5879168.1"
                     /translation="MARQQTKKNYSLRKLKTGTASVAVALTVLGAGFANQTEVRADEP
                     KKMEVKESEKESQYKTLALRGENADLRNVNAKYLEKINAEEEKNKKLEATNKELNENY
                     YKLQDGIDALEKENEDLKTTLAKTTKENEISEASRKGLSRDLEASRAAKKELEAKHQK
                     LEAENKKLTEANQISEASRKGLSNDLEASRAAKKELE"
BASE COUNT          374 a          138 c          176 g          204 t
ORIGIN      
        1 ggcgaaacag ctaacccatt ctttacagca gcagctgcaa cagtgatggt atctgcaggt
       61 atgcttgctc taaaacgcaa agaagaaaac taagcattag actgatgcta aagctaagag
      121 agaatcgaat gattctctct ttttgagtgg ttaagtaact aataatctca gttagaccaa
      181 aaaatgggaa tggctcaaaa agctggcctt tactcctttt gattaaccat atataataaa
      241 aacattagga aaataatagt aatattaagt ttgtttcctc aataaaatca aggagtagat
      301 aatggctaga caacaaacaa agaaaaatta ttcactacgg aaactaaaaa ccggtacggc
      361 ttcagtagcc gttgctttga ccgttttggg cgcaggtttt gcaaaccaaa cggaagtaag
      421 agctgatgaa cctaaaaaaa tggaagtaaa agaaagtgaa aaagagtccc agtataagac
      481 gttggcttta agaggtgaaa atgctgacct tagaaatgta aatgcaaaat atttagagaa
      541 aattaacgca gaagaagaaa aaaataaaaa gcttgaagca actaataaag agctaaatga
      601 gaattattac aaattacaag atggtattga tgctctagaa aaagaaaacg aagatctcaa
      661 aacaacttta gctaagacta ctaaagaaaa tgagatttca gaagctagcc gtaaagggtt
      721 gagccgagac ttagaagctt ctcgtgcagc taaaaaagag ctagaagcta agcatcaaaa
      781 attagaagca gaaaacaaaa aactaacaga agccaatcag atttcagaag ctagtcgtaa
      841 aggtctaagt aacgacttag aagcttctcg tgcagctaaa aaagaactag aa
//
LOCUS       DAUVUY010000182          884 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.184, whole genome shotgun sequence.
ACCESSION   DAUVUY010000182 DAUVUY010000000
VERSION     DAUVUY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.184"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            106..519
                     /locus_tag="VOX58_001984"
     CDS             106..519
                     /locus_tag="VOX58_001984"
                     /inference="COORDINATES: protein motif:HMM:NF013537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879169.1"
                     /translation="MGTAVAYAEEIPTTPILTLEVTERREAINKINRLSDQYPNDPEI
                     QATFEDTTNKILAFESADITEALIAEVNSEVTRLNEEKIARDAVDTAEKADAEKRHDF
                     LKQQDAVVIAIQELANIPAEKAFRQYANDNGVDGV"
     gene            640..831
                     /gene="grab"
                     /locus_tag="VOX58_001985"
                     /pseudo
     CDS             640..831
                     /gene="grab"
                     /locus_tag="VOX58_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G-related alpha2-macroglobulin-binding protein
                     GRAB"
BASE COUNT          323 a          143 c          165 g          253 t
ORIGIN      
        1 aattaaatta aggtagctat atactaatga tattattgta ataataattt gattaactaa
       61 tagtaaaagt taactgttta actttaatcg tctgctgcgt ttgtagtggg gacagcagta
      121 gcatacgcag aagaaatccc tactactcca atactcactt tagaggtgac tgagcgaaga
      181 gaagcgatta ataaaattaa cagattaagt gatcagtatc ctaacgaccc tgagattcag
      241 gcaacttttg aagatacgac taataaaatt ttagcatttg aatcagctga tattactgaa
      301 gcacttattg ctgaagtaaa ttctgaggtt actcgattga atgaagaaaa aatagcacgt
      361 gatgcagtag atacagctga aaaagcagat gcagaaaaac gtcacgactt cttgaaacaa
      421 caagacgctg ttgttattgc tattcaagaa ttggcaaata ttcctgctga aaaagccttc
      481 agacaatatg ctaatgataa tggtgttgac ggtgtttgat cttacgacga cgctactaag
      541 acgtttacag taacagaaat agttactgaa attcctggag atgctctagt tgaaccagaa
      601 aaactaaaag cagaaattcc ttttgttcca ttaacaccag caacttcaat cgctttagat
      661 gttaagaaaa ctaaagatac aaaacctgta gttaaaaaag aagaaagaca aaacgttaat
      721 acccttccta caactggtga agagtctaac ccattcttta cagctgctgc gcttgcaata
      781 atggtaagta caggtgtgtt agttgtaagt tcaaagtgca aagaaaatta gttgctattt
      841 gttctagcaa atgaaacaag ggaatcgaaa gattctcttt ttct
//
LOCUS       DAUVUY010000183          865 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.185, whole genome shotgun sequence.
ACCESSION   DAUVUY010000183 DAUVUY010000000
VERSION     DAUVUY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.185"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>865
                     /locus_tag="VOX58_001986"
     CDS             <1..>865
                     /locus_tag="VOX58_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HES5879170.1"
                     /translation="KAETAGVTNGTHLTIKKTIVNYNDSEVLMPKTTFTFVVKPLEVT
                     GKEKDTASGLKIQKGIDGVKSQEIKYDNAVKPKDKEKSVELDFSTVQFPNVGIYRYEV
                     SEQAGDVKGITYDSKKWTVDVYVVNKVGGGFEPRYIVSRESGKDEKKPILFENSFDTT
                     SLTVKKRVDGNTGDKQQEFEFKLLLEENDYFVKGQKVTLNKTTAEGVIENIQVPIGEE
                     YTFKLKDNEQVKLDKLPVGIKYKVDEVSKNQDNYQTTATIKQGDEVAEKYDLYSEKET
                     DESADEIVVTN"
BASE COUNT          354 a          107 c          179 g          225 t
ORIGIN      
        1 taaaagctga gacggcaggg gtgactaatg gaacacattt gactatcaaa aaaacaattg
       61 ttaattataa tgactcagaa gttctaatgc ctaaaactac atttactttt gtggttaaac
      121 cgcttgaggt aacaggaaaa gaaaaggata cagcttcagg cttaaagatt caaaaaggta
      181 ttgatggagt aaaatcgcag gaaatcaaat acgataatgc tgttaagcca aaggacaaag
      241 agaagtcagt agaacttgat ttttctacag tacaatttcc aaatgtaggg atttaccgat
      301 atgaagtttc agaacaagct ggtgacgtga aggggattac ttatgattct aaaaagtgga
      361 cagtagatgt ttatgtagtt aataaagttg gtggtggatt tgaaccaaga tatatcgtat
      421 ctagagagtc aggaaaagat gagaaaaaac caattttatt tgaaaattct tttgatacaa
      481 catctttaac tgttaaaaag agagtagatg gaaatactgg agataaacaa caggagtttg
      541 agtttaaatt attattggaa gaaaatgact atttcgtgaa aggtcaaaaa gtaacattga
      601 acaaaaccac tgctgaaggg gtaatagaaa atattcaagt accgattgga gaagagtata
      661 cattcaaatt gaaagacaat gaacaggtta agttagacaa actacctgtt ggtattaaat
      721 ataaagtaga tgaagttagc aaaaatcaag ataactatca aactacagct actatcaaac
      781 aaggggacga agtagcagaa aagtatgatt tatactcaga gaaagaaaca gacgaatctg
      841 ctgacgaaat cgttgtcaca aataa
//
LOCUS       DAUVUY010000184          828 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.186, whole genome shotgun sequence.
ACCESSION   DAUVUY010000184 DAUVUY010000000
VERSION     DAUVUY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.186"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(21..602)
                     /locus_tag="VOX58_001987"
     CDS             complement(21..602)
                     /locus_tag="VOX58_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HES5879171.1"
                     /translation="MHNEKSNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLCRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQER
                     MQFQALIRGERFKGRRALLRKISPYFKEFAQQL"
     gene            complement(598..671)
                     /locus_tag="VOX58_001988"
     tRNA            complement(598..671)
                     /locus_tag="VOX58_001988"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(635..637),aa:Arg,seq:acg)
     gene            complement(751..824)
                     /locus_tag="VOX58_001989"
     tRNA            complement(751..824)
                     /locus_tag="VOX58_001989"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Asn,seq:gtt)
BASE COUNT          235 a          200 c          128 g          265 t
ORIGIN      
        1 taattcctta taggtcttgc ttacaactgc tgtgcaaatt ccttaaaata gggactgatc
       61 ttcctaagta gagcacgtct tcccttgaaa cgttcgcccc tgataagtgc ttgaaactgc
      121 atacgctctt gcgaatttaa ttggttctct aagctagcta ttacctcttg ataagccaca
      181 tagtcgtcta gccataaccc tctcgatgga atggcatgtg caacctcccc tatctcttca
      241 tatgctaact tatggaactg acgcttttga ctttcttgac ggcataataa atctttcaaa
      301 taagatgaaa attttgtttt aaaatagcga tataaacgct cttcttcctc aattaactct
      361 gggtaccttt ctagtaactg taataagata atatgtcctt cttgtaacca gtcatctcta
      421 tcccacaatt gaatataata gtgtcgcttt aatttcaaaa taatgggctt aactttttca
      481 aacgctgctc ttgtctctat cgacattttg agtctccttt tctttttaac tatccctagt
      541 ataaagagaa ccacaaaaaa acaacaggac actaatgtcc tgttgctttt ttcattatgc
      601 accctagagg agtcgaacct ctaaccgcct gattcgtagt caggtactct atccagttga
      661 gctaagggtg ccaacgcata tcttgcctct atcttaccac actcttctct aaaatgcaaa
      721 aaaagatagg acgcttccta cctctctcta ctccgccagt aggactcgaa cctacgacat
      781 catgattaac agtcatgcgc tactaccaac tgagctatgg cggataaa
//
LOCUS       DAUVUY010000185          819 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.187, whole genome shotgun sequence.
ACCESSION   DAUVUY010000185 DAUVUY010000000
VERSION     DAUVUY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.187"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..>819)
                     /locus_tag="VOX58_001990"
     CDS             complement(<1..>819)
                     /locus_tag="VOX58_001990"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HES5879172.1"
                     /translation="GIVTAIYDVPVGQAWPLENILALKKMVEDAGLEISVIESIPVHE
                     DIKQGKPTRDALIENYKTSITNVGKAGIPVVCYNFMPVFDWTRSDLNHPLPDGSTSLA
                     FLKADLEGVDPVADDLNLPGWDSSYSKEEMKAIIENYRNNISEEDLWVNLDYFIKAIM
                     PTAEAAGVKMAIHPDDPPYGIFGLPRIITGQDAVERFLNLYDSPNNGITMCVGSYASD
                     PKNDVIAMTEYALKRNRINFMHTRNVTAGAWGFQETAHLSQAGDIDMNAVVKLL"
BASE COUNT          232 a          184 c          171 g          232 t
ORIGIN      
        1 ccaataattt gacaacagca ttcatgtcaa tatcaccagc ttgtgacagg tgagctgttt
       61 cttggaagcc ccaagcgccg gctgtcacgt tccgcgtgtg cataaagttg atacggttac
      121 gtttaagagc gtactcagtc atggcaatca catcattttt aggatcagaa gcatacgacc
      181 ctacacacat ggtaatccca ttgtttggag aatcatagag attcaagaag cgctcaacag
      241 catcttgacc tgttataata cgtggcaaac caaagatacc atatggtggg tcatcagggt
      301 ggatcgccat tttcacacca gctgcttctg cggttggcat aatagctttg atgaagtagt
      361 ctaggtttac ccataaatct tcttcagaga tattgttgcg gtaattctcg atgatggctt
      421 tcatttcttc cttagaataa gaagaatccc aacctggtag attcaaatcg tcagctactg
      481 gatcaacacc ctctaaatca gcttttaaga aagcaagcga tgtggatccg tccggtagtg
      541 gatggttaag gtctgaacgt gtccagtcaa aaactggcat aaagttgtaa caaaccaccg
      601 gaatacctgc tttacccacg ttggtaatag aggttttata gttttcaatc aatgcatcac
      661 gcgttggctt gccttgtttg atgtcttcat gaactgggat agattcaata actgaaatct
      721 ctaagccagc atcttcgacc attttcttca aggcgagaat attctcaagt ggccaagctt
      781 gaccaaccgg cacatcgtaa atcgccgtaa caattccct
//
LOCUS       DAUVUY010000186          808 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.188, whole genome shotgun sequence.
ACCESSION   DAUVUY010000186 DAUVUY010000000
VERSION     DAUVUY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.188"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..628
                     /locus_tag="VOX58_001991"
     rRNA            <1..628
                     /locus_tag="VOX58_001991"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            714..>808
                     /gene="rrf"
                     /locus_tag="VOX58_001992"
     rRNA            714..>808
                     /gene="rrf"
                     /locus_tag="VOX58_001992"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          217 a          172 c          233 g          186 t
ORIGIN      
        1 aacggaggcg cccaaaggtt ccctcagatt ggttggaaat caatcgcaga gtgtaaaggt
       61 ataagggagc ttgactgcga gagctacaac tcgagcaggg acgaaagtcg ggcttagtga
      121 tccggtggta ccgaatggaa gggccatcgc tcaacggata aaagctaccc tggggataac
      181 aggcttatct cccccaagag ttcacatcga cggggaggtt tggcacctcg atgtcggctc
      241 gtcgcatcct ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca
      301 cgcgagctgg gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgtagga
      361 aatttgagag gatctgctcc tagtacgaga ggaccagagt ggacttaccg ctggtgtacc
      421 agttgtcttg ccaaaggcat cgctgggtag ctatgtaggg aagggataag cgctgaaagc
      481 atctaagtgc gaagcccccc tcaagatgag atttcccatg attttatatc agtaagagcc
      541 ctgagagatg atcaggtaga taggttagga gtgtaagtgt agcgatacat gtagcggact
      601 aatactaata gctcgaggac ttatccaaaa agaaatattg acaacgttac ggattcttgt
      661 tagactatag atattcaatt ttgagtgggt tatgaaaata gcacatttac aagttaagtg
      721 acgatagcct aggagataca cctgtaccca tgccgaacac agcagttaag ccctagaacg
      781 cctgatgtag ttgggggttg ccccctgt
//
LOCUS       DAUVUY010000187          807 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.189, whole genome shotgun sequence.
ACCESSION   DAUVUY010000187 DAUVUY010000000
VERSION     DAUVUY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.189"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            165..>807
                     /locus_tag="VOX58_001993"
     CDS             165..>807
                     /locus_tag="VOX58_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HES5879173.1"
                     /translation="MISYRKTFVAQIDARDCGVAALASIAKYYGSDYSLAHLRELAKT
                     NKEGTTALGIVKAAKLMGFETRAIQADMTLFDIEDVPYPFIVHVNKEGKFQHYYVIYQ
                     NKKNYLIIGDPDSTVKVTKMTKERFTSEWTGVAIFLAPEPSYKPHKDKKNGLISFLPL
                     IFKQRSLIFYIILASLLVTLINIVGSYYLQGILDDYIPNQLKSTLGIISIGLII"
BASE COUNT          266 a          152 c          124 g          265 t
ORIGIN      
        1 tgtcttttta tcctctctac acttacgctg tgacaatagt taattaatta agaagtttaa
       61 ataataccat tcatgctttt ataaatacct tttaagattt ttgataaaaa ctccctatat
      121 atatgtcata atagcttagt atttaataga aaggtctttt acaaatgata tcctatcgaa
      181 aaacatttgt tgctcagatt gatgctagag actgtggtgt ggctgctctt gcttccatcg
      241 ctaaatatta cggttcagac tactctttag ctcatttaag agagttagct aaaacaaata
      301 aagagggaac aacagcgctt ggcatcgtca aagctgccaa attaatggga tttgaaacaa
      361 gagctattca agcagacatg accctctttg atatagaaga tgtcccttat ccttttattg
      421 ttcatgttaa taaagaaggt aaattccaac actactatgt catctatcaa aacaaaaaaa
      481 attatctgat aatcggcgac cctgactcga cagtaaaagt cactaagatg acaaaggagc
      541 gatttacttc tgaatggacg ggtgtcgcta tttttctagc acccgagccc agctacaagc
      601 ctcataagga caaaaagaac ggtctaataa gctttttacc tctcattttt aaacagcgat
      661 ctctgatttt ctatatcata ttagctagcc tactcgttac tctgattaat attgtaggtt
      721 cttattacct acaaggtata ttagatgact atattccaaa tcaactgaaa tcaacactcg
      781 gcattatttc aattggtctt atcatta
//
LOCUS       DAUVUY010000188          792 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.190, whole genome shotgun sequence.
ACCESSION   DAUVUY010000188 DAUVUY010000000
VERSION     DAUVUY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.190"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>792
                     /locus_tag="VOX58_001994"
     CDS             <1..>792
                     /locus_tag="VOX58_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin-binding protein"
                     /protein_id="HES5879174.1"
                     /translation="RVKKELDALKENSPKLGERSLYFRLVNKFLSWFGQVWDPLAIKE
                     TDHLLDLIGQVNYLVSNTEFQAPEYQKAKNKLDLAVAGYELYLAREKERGDAPDYQVT
                     QLTLAQLKTIKTKIQEALKTFAQEVKTIQQTHPDLFPHAYVLNATTSAIDGKAYTFLV
                     NSYYDVFYKQFLFTNTKDGYNYNKEADNLTSYQDLIKELLDLKAEIDQLTKDKPTNDR
                     LETLMWESKFTLSDSRRTSRLSAYYTQEDYVNKVVLSEEAPNLRTA"
BASE COUNT          283 a          172 c          148 g          189 t
ORIGIN      
        1 acgtgtcaaa aaagaactag acgcattaaa agaaaatagc ccaaaattag gagaacgttc
       61 cctttatttt agattggtga ataaatttct aagttggttt ggccaagtct gggacccact
      121 cgcaatcaaa gaaaccgatc acctgttgga cctcatcgga caagtcaatt acttggtgag
      181 taacaccgag tttcaagccc ctgagtacca aaaggctaaa aacaaactag acttagctgt
      241 agcaggctat gagctctacc tagctagaga aaaagaacgt ggtgatgctc ctgattacca
      301 agtgacccag ctaacgctag cacagctcaa aaccatcaaa acgaagatac aagaggccct
      361 gaaaacattt gcacaagagg tcaaaacaat ccaacaaaca cacccagacc tatttcccca
      421 tgcttatgtt ttaaacgcta caaccagtgc tatcgatggt aaagcgtaca cttttttagt
      481 gaatagctac tacgatgtct tttataagca gtttttattc actaatacaa aggacgggta
      541 caactataat aaagaagcag acaatctgac ctcgtatcaa gatctgatta aagagctatt
      601 agacttaaaa gctgagatcg atcagttaac aaaagacaag cctacaaacg atcgtttaga
      661 gacgttaatg tgggaatcaa agttcacctt atctgattca cggagaactt cacgccttag
      721 cgcctactat acacaagaag attatgtcaa taaggtggtc ctatctgaag aagctcccaa
      781 cttgagaaca gc
//
LOCUS       DAUVUY010000189          783 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.191, whole genome shotgun sequence.
ACCESSION   DAUVUY010000189 DAUVUY010000000
VERSION     DAUVUY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.191"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..409)
                     /locus_tag="VOX58_001995"
     CDS             complement(<1..409)
                     /locus_tag="VOX58_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HES5879175.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAIIVNLHLLFALTIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSTMLADPEAKITSLLGTQMIVKDYFT"
BASE COUNT          244 a          170 c          138 g          231 t
ORIGIN      
        1 tggtgaaata atctttaacg atcatttgag tgccaaggag gcttgtgatt tttgcttctg
       61 gatcagctag catagtacta gacacgccat aaaaagcgcc tgttatccga ttgatcaaga
      121 caaaagcaag acctgaagca aaggctcctc ctgcacgttc tttagcccaa ctaccaccaa
      181 tggtcaaggc aaatagcaag tgaaggttaa caatgatagc ccaaccaatc tgagcaataa
      241 tgtttcctag agatgccaaa aatgctgagt cgtggttaat cattggaata gagtttccga
      301 tactgatcat taaaccagct gctggcatga cagcaataac gaccataaga catttcccaa
      361 atttttgcca aaattcgaaa cgaaacagtt gcttgaatga tgttttcatt ctatctctcc
      421 tttattatcc ccttttagcc atagctgccc ttaaggcgct tttgtgacac aatgatatag
      481 actacaactc agcgatgcaa tcgcttgcat tgatctgata cctttattat actagtctcg
      541 caacaatttg taaagcgttt tcttagacag atgttagtcg tttttcttca ttaatatagt
      601 agtgtaactc accaaaaaga tgacaagagc ccatttcctt ttttggtgat gagcccatca
      661 acgatcttca aaaaaacaag aaacaataaa aaaaggccgt tgacacaatc gtgaactgcc
      721 tgttcgtttc tttagcctct tctttctaaa gtaagcaaca gcctacttat tttgatgcct
      781 gca
//
LOCUS       DAUVUY010000190          769 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.192, whole genome shotgun sequence.
ACCESSION   DAUVUY010000190 DAUVUY010000000
VERSION     DAUVUY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.192"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..183
                     /locus_tag="VOX58_001996"
     CDS             <1..183
                     /locus_tag="VOX58_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HES5879176.1"
                     /translation="GEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            192..>769
                     /locus_tag="VOX58_001997"
     CDS             192..>769
                     /locus_tag="VOX58_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 family protein"
                     /protein_id="HES5879177.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAEQSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLA"
BASE COUNT          216 a          139 c          174 g          240 t
ORIGIN      
        1 ggagaaggag ccaactgcaa aggctacctc ttgtggactt ttattgattg ctggtcttgg
       61 ttaaatgctt ataaaaaccg atatggtttg gtagctttgg atttagagag tcaaaagcga
      121 accataaaaa aatcaggtta ctggtttaag gcgttgagcg agtcaaatgg ttttgataaa
      181 taggaggcaa tatggttgat ttaggatttt ctctataccc tgagcggtat gatgttacca
      241 agagtaaagc ttatattgac ttatgtcata gctatggagc caagcgcttg tttatgagtc
      301 tcttacagct ggcaccagct gatcaccaga tgtttcattg ctacgcagag ctcattgctt
      361 atgcaaatca attaggcatt cgagtaattg ctgacgttag tccaagcttt attagccaag
      421 ctggttggtc agatcagctt attgaacgag ctcatgcctt cgggttagcg ggcttacgct
      481 tagacgaggc tctccctcta gcagagattg taactttaac aagaaatccc tttgggctta
      541 aaattgaact taatatgagc actgataaac aattattgat gtcattgcta gcgacagatg
      601 cagaacagag caatattatt ggttgccata atttttatcc acatgaattt accggtttgt
      661 catggcaaca ttttaaggat atgtcacgtt tttatcatga gcatgatatt gaaacggctg
      721 cttttattac cgctcagtca gcatctgaag gcccttggct actggcaga
//
LOCUS       DAUVUY010000191          763 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.193, whole genome shotgun sequence.
ACCESSION   DAUVUY010000191 DAUVUY010000000
VERSION     DAUVUY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.193"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..222
                     /locus_tag="VOX58_001998"
     CDS             <1..222
                     /locus_tag="VOX58_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HES5879178.1"
                     /translation="EAGKRVPEDFEIITSNDSPVVQYTRPNLSSISQPVYDLGAVSMR
                     MLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            353..>763
                     /locus_tag="VOX58_001999"
     CDS             353..>763
                     /locus_tag="VOX58_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HES5879179.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGID"
BASE COUNT          261 a          124 c          141 g          237 t
ORIGIN      
        1 gaagcaggta aacgcgtacc agaggacttt gaaatcatca ccagcaacga ttcaccagtg
       61 gttcaataca ctcgtcccaa tttgagttct atcagtcaac ctgtttatga cttaggtgct
      121 gttagcatgc ggatgttgac taaaatcatg aacaaagaag agttggaaga aaaagaaatt
      181 cttttgaatc atggtattaa aaaacgtggg acaactaagt aactgtgact tatgagcacc
      241 atctaacaaa aagtatcaca atagactgtt tcttcaatac taaaatcagc cttagggctg
      301 atttttgcat gtcttagtga aaaatactat aaaatgagct ataatagaac ttatgaaagt
      361 cttattgtat ttagaagcag aaaattatct aagaaaatca ggaattggtc gagcgattaa
      421 gcatcaggct aaagccttgt cacttgttgg tcaacatttt acgactaatc caagagaaac
      481 ttatgatttg gttcatctca atacctatgg tttaaaaagt tggctgctga tgataaaagc
      541 acaaaaagct ggtaagaagg ttatcatgca tgggcattct acagaagaag attttagaaa
      601 ttcttttatt ttttcaaatc tattatctcc ttggtttaaa aaataccttt gtcactttta
      661 caataaggca gatgctatca ttacccctac cctatattct aagtctttga ttgagagtta
      721 tggagtgaag tcacctattt ttgcagtgtc aaatgggatt gat
//
LOCUS       DAUVUY010000192          736 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.194, whole genome shotgun sequence.
ACCESSION   DAUVUY010000192 DAUVUY010000000
VERSION     DAUVUY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.194"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..399
                     /locus_tag="VOX58_002000"
     CDS             <1..399
                     /locus_tag="VOX58_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HES5879180.1"
                     /translation="YAWYVDHGDLAKGEADLRKELLEWQSKFPDKPILMTEYGADTLP
                     GLHSMWDIPYTEEFQCDFYAMTHGVFDEIPNLVGEQVWNFADFETNLMIFRVQGNHKG
                     LFSRNRQPKQVVREFKKRWTAIPNYNYKKK"
     gene            441..>736
                     /locus_tag="VOX58_002001"
     CDS             441..>736
                     /locus_tag="VOX58_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HES5879181.1"
                     /translation="MARPLVEKTAERLLQLILERGYDVGAKLPNEYELAQDLDVGRST
                     IREAVRSLATRNVLEVRQGSGTYVSSKKGVSEDPLGFSLVKDTAKLTADLFELR"
BASE COUNT          230 a          125 c          185 g          196 t
ORIGIN      
        1 tacgcttggt atgtggatca cggtgatctg gctaagggag aagctgacct acgcaaggaa
       61 ttgttagaat ggcagagcaa gttcccagac aaacccattt tgatgaccga gtatggggca
      121 gataccttgc ctggcttgca ttcgatgtgg gatattccgt atacggaaga attccaatgt
      181 gatttttatg cgatgacgca tggagttttt gatgagattc ctaaccttgt tggtgaacaa
      241 gtttggaact ttgcagactt tgagaccaat ttaatgattt tccgtgtgca aggtaaccat
      301 aaaggactct tctcgcggaa ccgtcagcct aaacaagtgg tcagagaatt taaaaaacgt
      361 tggacagcta ttcctaacta caattacaag aaaaaatagt gtaaaatagg agacagtaga
      421 aacaggaaga ggactatacg atggctagac cattagttga aaaaacagca gaacgtttat
      481 tacagcttat tttagaacgt ggatatgacg taggggctaa gctccctaat gagtacgaat
      541 tagctcaaga cttggatgta ggacgtagca ctatccgaga ggctgtcaga agtttagcaa
      601 cccgcaatgt cttagaagtg agacagggtt ctggaaccta tgtgagttct aaaaaagggg
      661 tatcagaaga cccgcttggt ttttcattgg tgaaagatac ggcaaaattg acagcagatt
      721 tgtttgaatt acggtt
//
LOCUS       DAUVUY010000193          727 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.195, whole genome shotgun sequence.
ACCESSION   DAUVUY010000193 DAUVUY010000000
VERSION     DAUVUY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.195"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>727
                     /locus_tag="VOX58_002002"
     CDS             <1..>727
                     /locus_tag="VOX58_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HES5879182.1"
                     /translation="EPTLLVPMGLGTILVNFPGSGVLTQVVNGVEQEGVFDALFNFGI
                     GTELFPLLIFIGIGAMIDFGPLLQNPFMLLFGAAAQFGIFFVVVAVLAGFDIKEAASI
                     GIIGAADGPTSIFVTNQLAKDLLGPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRM
                     TYKAENVSQMTKILFPIIITLVAGFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQN
                     ELVNIISILLGLTISIKMQADLF"
BASE COUNT          179 a          155 c          152 g          241 t
ORIGIN      
        1 acgaaccaac tctacttgtg ccaatggggc ttggaacaat ccttgtgaat ttccctggct
       61 caggggtttt aactcaagtg gttaacggag tcgagcaaga aggggttttt gatgccctct
      121 tcaattttgg gattggaaca gaacttttcc cactgttgat tttcattggt attggcgcca
      181 tgattgactt tggtccttta cttcaaaacc cattcatgct cttgtttggt gctgcagcgc
      241 aatttgggat tttctttgtt gttgttgccg tactggctgg atttgacatt aaagaagcag
      301 cttcaatcgg cattatcgga gcagcagatg gaccgacttc tatctttgtt accaaccaac
      361 tggccaagga tttactgggt ccaattacgg ttgcagccta ttcttatatg gccctggttc
      421 ctattatcca accatttgcc attaaattag tgacaacgaa aaaagagcgc cgtatccgta
      481 tgacttacaa agctgagaat gtatctcaaa tgactaaaat cttattccca atcatcatta
      541 ccttagtagc agggttcatc gccccaattt ctctaccttt agttggcttt ttgatgttcg
      601 gtaacttatt acgtgagtgt ggtgtgctcg atcgcctgtc acaaactgct caaaatgaat
      661 tggtaaacat tattagtatc ttgcttggat tgaccatctc tattaaaatg caggcagacc
      721 tgttctt
//
LOCUS       DAUVUY010000194          700 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.196, whole genome shotgun sequence.
ACCESSION   DAUVUY010000194 DAUVUY010000000
VERSION     DAUVUY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.196"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            55..>700
                     /locus_tag="VOX58_002003"
     CDS             55..>700
                     /locus_tag="VOX58_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HES5879183.1"
                     /translation="MKNISRKCFMTSVVCIVLGAILLGAGYATGGLQDIKRQTAPKKV
                     IKTFDQITALDIDSSASTITVETGPVQRPTVTYYTHPKFIDPIVTTVTGKTLSLSQKP
                     KDVVITGGIEILGFTLNNSRQEKNYRSITITVPEKTSLNEVKASNVPHTTLSNLTVQD
                     MQFDGNLTLLHTKVKKATITGMLEATKSQLTNLELKADYSFSNLTDSSVENGTIS"
BASE COUNT          228 a          150 c          123 g          199 t
ORIGIN      
        1 acgggctttt gttgccttta ttaagtggat tagtcacaag aaaggagctc aggcatgaaa
       61 aatatctcac gcaaatgttt tatgaccagt gtcgtgtgta ttgttttagg agccattctt
      121 ttaggagctg gttatgcaac tggcgggctt caagacatta aacgccaaac agcccccaaa
      181 aaggtcatca aaacatttga ccaaataact gctcttgata ttgacagttc tgcctcaact
      241 attacagtag agacaggacc cgttcaaaga ccaacagtga cctattacac acatcctaaa
      301 tttattgacc ctatcgtcac aacagtaaca ggtaagaccc tatctctttc gcaaaaacct
      361 aaagacgttg tcatcactgg tggaattgaa attttaggtt ttactctcaa taatagtcgt
      421 caagagaaga actaccgttc tattaccatt actgttcctg aaaaaactag cctcaatgaa
      481 gttaaggcaa gtaatgtccc acataccact ttgtcaaatc taactgtcca agatatgcaa
      541 ttcgatggca atcttactct tttacatacc aaagtcaaga aagctactat cactggtatg
      601 ttggaagcca ctaaaagtca gttaacaaat ctcgagttaa aagctgacta ttctttttca
      661 aacctgactg attctagtgt ggaaaatggg accatcagct
//
LOCUS       DAUVUY010000195          699 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.197, whole genome shotgun sequence.
ACCESSION   DAUVUY010000195 DAUVUY010000000
VERSION     DAUVUY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.197"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..246
                     /locus_tag="VOX58_002004"
     CDS             <1..246
                     /locus_tag="VOX58_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HES5879184.1"
                     /translation="EADVIVANILADILVLLTDDAYRLVKKEGYLILSGIISEKLDMV
                     LEAAFSAGFFLETHMVQGEWNALVFKKTDDISGVIGG"
     gene            246..>699
                     /locus_tag="VOX58_002005"
     CDS             246..>699
                     /locus_tag="VOX58_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HES5879185.1"
                     /translation="MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLTDEAEVVLVFDDG
                     VKYLAKVTSSMAHELEIIETLPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEQK"
BASE COUNT          215 a          111 c          182 g          191 t
ORIGIN      
        1 gaagcggatg tgatcgtagc taatattttg gcggatatct tagtcttatt gactgatgat
       61 gcttaccgtt tggtcaaaaa ggaaggttac cttatcttat caggcatcat ttctgagaaa
      121 ttggatatgg ttctggaagc tgcctttagc gcaggctttt tcttagaaac tcacatggtt
      181 caaggcgagt ggaatgccct tgtctttaaa aaaacagatg atatctcagg agtgattggc
      241 ggctaatgca acagtatttt attaaaggca aagcagaaaa aaaggtaaca attaccgata
      301 aagataccat caagcacatg tttcaagtga tgcgcttgac tgacgaggca gaagttgtct
      361 tggtctttga tgatggggtg aagtatttag ccaaggtaac cagtagtatg gctcatgagt
      421 tagagattat agagaccttg ccagatcagg tggaattgcc tgttaaggtg actattgctt
      481 ctggatttcc taaaggcgat aagttagata cgattgccca gaaagtgact gaattgggag
      541 ccagtgcgct atggggctac ccagcagact ggtcagtggt caaatgggat ggcaaaaaat
      601 tagctaaaaa agaagacaaa ttggctaaga ttgtattagg agcggctgag cagagcaaac
      661 gaaatagggt gcctgaagtt cacctttttg aacagaaag
//
LOCUS       DAUVUY010000196          654 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.198, whole genome shotgun sequence.
ACCESSION   DAUVUY010000196 DAUVUY010000000
VERSION     DAUVUY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.198"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(77..>654)
                     /locus_tag="VOX58_002006"
     CDS             complement(77..>654)
                     /locus_tag="VOX58_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HES5879186.1"
                     /translation="LGTLIWSVLAGLILSLFAHAYKHLLEHLPKADAKSQWFIPKVLI
                     AFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKAVAISLVFASGAKGG
                     KIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGVINKIPLAAPVFLVE
                     ITGQSLLMIIPLALANLIFYFSYQFYRFILK"
BASE COUNT          236 a          119 c          137 g          162 t
ORIGIN      
        1 cacttaattt tgtgtctact ttagtatacc aagtccaatt catccgaagc tttttattaa
       61 aaaagtaatt aactaatcat ttaaggatga aacgataaaa ctgatagcta aaataaaaaa
      121 tgaggttagc taatgctaaa gggataatca ttagtaacga ttggcctgta atttcaacca
      181 gaaaaactgg tgccgccaag ggtatcttat tgatcacacc taaaaagatt gttgcaccaa
      241 ccataatggc taacgtattt gatagagaca agggaataag gtactgacta aggatagcta
      301 gtagtaagcc actagcacct ccaagcatca tagaaggagc aattttaccc ccttttgctc
      361 cagaagcaaa aactaaagag atagcgacag ctttggcaac aagtagccag ctaatatagc
      421 tcagatgtgg ttcttcatgg agaaaaaaga gtaatccagc tttgccattt cctaaaatct
      481 ctgggaagaa aatgctgagt cctgcgataa ggctaaaagc gatgagaact ttcggaataa
      541 accactgact tttggcatca gccttaggaa ggtgttccaa aagatgtttg taagcgtgag
      601 caaagaggga aaggataaga ccagccagga cggaccaaat aagggttcct agcg
//
LOCUS       DAUVUY010000197          631 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.199, whole genome shotgun sequence.
ACCESSION   DAUVUY010000197 DAUVUY010000000
VERSION     DAUVUY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.199"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>631
                     /locus_tag="VOX58_002007"
     CDS             <1..>631
                     /locus_tag="VOX58_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HES5879187.1"
                     /translation="LNSFSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTIL
                     MRVKEGAADYRYFPEPDLPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDAS
                     QLTATKALSDFFETAVSLGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMI
                     AIIADGTISSKMAKKVFVHLAKNGGSARAYVEKAGLVQISDPAVLVPIIH"
BASE COUNT          176 a          136 c          152 g          167 t
ORIGIN      
        1 cttgaactcc ttctcaaatg tccgtaaagg gcttgaattt gaggtggaac gtcaagccaa
       61 actcttgcgt tcaggtgggg tgatacgcca agaaacgcgc cgctatgacg aagcgaataa
      121 ggggactatt ttgatgcggg tcaaagaagg ggcagcggac tatcgttatt tcccagaacc
      181 agacctaccc ttatatgaaa ttgatgatgc ttggattgac gagatgcgtg ctcaattacc
      241 tcaattccca gctcaacgtc gtgctaaata cgaagaggaa cttggcctct cagcttatga
      301 tgctagccag ctaacagcta ccaaagcctt gtcagatttc tttgaaactg cagtaagtct
      361 tggtggcgat gccaagcaag tctctaactg gttgcaagga gaagttgccc aattcttaaa
      421 tgcagaaggc aaaaccattg aagaaatcgc tctgacacca gaaaatttgg tggagatgat
      481 tgctatcatt gctgatggta caatctcttc taaaatggct aagaaggtct ttgtgcattt
      541 ggctaaaaat ggtggctcag ctcgcgccta cgtcgaaaag gcaggacttg ttcagatttc
      601 tgatcccgct gttttggtac caattattca c
//
LOCUS       DAUVUY010000198          606 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.200, whole genome shotgun sequence.
ACCESSION   DAUVUY010000198 DAUVUY010000000
VERSION     DAUVUY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.200"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..452
                     /locus_tag="VOX58_002008"
     CDS             <1..452
                     /locus_tag="VOX58_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HES5879188.1"
                     /translation="ITLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQ
                     AKAMYNLPAFPLLYEVSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTK
                     MVYDNYKATKGPKEILIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            506..591
                     /locus_tag="VOX58_002009"
     tRNA            506..591
                     /locus_tag="VOX58_002009"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Leu,seq:aag)
BASE COUNT          184 a          117 c          139 g          166 t
ORIGIN      
        1 aaatcacact ctttggctta tctatgggtg ctgcaacagt aatgatggca agtggtgagc
       61 gattgcctgc gcaagtcacc tccctcatcg aagattgcgg ttataccagt gtttgggacg
      121 aattgaagtt tcaggccaag gctatgtaca acttgcctgc ctttccttta ctctatgaag
      181 tctctgcctt atctaagatt cgagcaggtt ttagttacgg agaagcgagc tcagtgaaac
      241 agctggctaa aaataaacgt ccaactttat ttatccacgg tgataaggat gattttgttc
      301 ctacaaaaat ggtttatgac aattataagg ccacgaaagg tcctaaggaa atcttgattg
      361 ttaaaggggc aaaacacgcc aaatcctttg aaacaaaccc agaacaatac cagaaaaaaa
      421 ttgccgcttt tttgaaaaaa gttgagaaat aagtattgac agttaaactc acagttgcta
      481 taataattta tgttactgat gagtagcggg gatggcggaa ttggcagacg cgcaggacta
      541 aggatcctgt gaccgcttta ggtcgtgtgg gttcaagtcc cactctccgc ataatgtaag
      601 aggtga
//
LOCUS       DAUVUY010000199          604 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.201, whole genome shotgun sequence.
ACCESSION   DAUVUY010000199 DAUVUY010000000
VERSION     DAUVUY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.201"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            384..>604
                     /locus_tag="VOX58_002010"
     CDS             384..>604
                     /locus_tag="VOX58_002010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879189.1"
                     /translation="MFLKHQDVKQKNWRMRKVKKLFVSSCMLLTVGLGVAVPTGFSQS
                     NGVMVVKAAEAEELPDDLMNFKGTWEVSA"
BASE COUNT          212 a           84 c          138 g          170 t
ORIGIN      
        1 cgcgtgttta aggagtattg agaatgtcag atataagcaa aatgcagctg aatattctta
       61 attttaggta tgaaaaagcg tcgggattga taaaaaatct attgaattgg gtaaatttaa
      121 gttgatataa taagttgatt agaaaaatta ataattcatt taaattctaa tcatctgtaa
      181 cgttccgggg cgtgttaaac tgtctgaaca tagaaaatac aggtttctaa ggtgactaag
      241 ataagctaag gaggattcct taaattctgt gataggaacg taaactctct acaagagtag
      301 agcacgtcat cagtcagtct gggtgtctac tggctgacaa cgtcaaataa gataggcatg
      361 aaaaaaataa gggggaacac agaatgtttt taaaacacca agatgtgaaa caaaaaaatt
      421 ggagaatgcg taaggtcaaa aaactctttg ttagttcatg tatgctttta acagtcggcc
      481 tcggagttgc cgtacctact ggattcagcc aatctaatgg cgtgatggtc gtaaaggctg
      541 cggaagctga agaactgcca gatgatttga tgaattttaa aggaacttgg gaagtatctg
      601 ccga
//
LOCUS       DAUVUY010000200          576 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.203, whole genome shotgun sequence.
ACCESSION   DAUVUY010000200 DAUVUY010000000
VERSION     DAUVUY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.203"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..570
                     /locus_tag="VOX58_002011"
     CDS             <1..570
                     /locus_tag="VOX58_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HES5879190.1"
                     /translation="YNPLSFNNKSSIIFEMKDPSLAKYWRLFNDNSKGKDDYIKEAKL
                     EVFTGQLNAEADVKTSLEKSDDWQTVSTYSGQEQVFSHALDNISAKYWRITVDNKKNQ
                     YGYVSLPELQILGYPLPNADTIMKTVTAAKELSQQKDKFSQKMLDELKIKEMALETSL
                     NSKIFDVTAINANAGVLKDCIEKRQLLKK"
BASE COUNT          218 a           89 c          107 g          162 t
ORIGIN      
        1 tataatcctt tatcgtttaa caataaatct tcgattatct ttgaaatgaa agaccctagt
       61 ttagcaaaat attggcgcct tttcaatgat aacagtaaag gcaaagacga ctatatcaaa
      121 gaagcaaaac ttgaagtctt tacaggccag ctgaatgctg aggcagacgt gaaaacgagc
      181 ttggaaaaat cagatgattg gcaaactgtt tcaacctatt caggacaaga acaggtattt
      241 agccatgcac tggataatat tagtgctaag tactggagaa taacagttga caataagaaa
      301 aatcaatatg gatatgtttc actccctgaa cttcaaattt taggttatcc gttacctaac
      361 gccgacacta tcatgaaaac agtaactgct gctaaagagt tgtctcaaca aaaagataag
      421 ttttctcaaa agatgcttga tgagttaaaa ataaaagaga tggctttaga aacttctttg
      481 aacagtaaga tttttgatgt aactgctatt aatgctaatg ctggagtttt gaaagattgt
      541 attgagaaaa ggcagctgct aaaaaaataa acaaag
//
LOCUS       DAUVUY010000201          555 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.204, whole genome shotgun sequence.
ACCESSION   DAUVUY010000201 DAUVUY010000000
VERSION     DAUVUY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.204"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..248)
                     /locus_tag="VOX58_002012"
     CDS             complement(<1..248)
                     /locus_tag="VOX58_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HES5879191.1"
                     /translation="MNHKNAIRKLKEFHRWQRIANSLNLTYNECYQFDIEYYSFRRKH
                     LEISRKCALEELDAIKHAINQLSKIEYRKILIECYLIG"
     gene            complement(238..>555)
                     /locus_tag="VOX58_002013"
     CDS             complement(238..>555)
                     /locus_tag="VOX58_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HES5879192.1"
                     /translation="KPLFALMTLFEEQRDKDIKLMNAFRNPELLNGIEKGTAKQLLYL
                     AKERDKRLAMIATLQDERQIAVIKARYVDDLSWDEIPDKLGYSRNTVFKLHREALEVL
                     DEP"
BASE COUNT          158 a          109 c           89 g          199 t
ORIGIN      
        1 tcaccgatca agtaacactc aatcagtatc tttctatact ctatcttaga tagttgatta
       61 atggcatgct taatagcatc tagctcctct agggcgcatt ttcggcttat ttcaaggtgt
      121 ttacgtctaa aggaataata ttctatatca aactggtaac actcgttata ggttaaatta
      181 aggctattag cgatacgttg ccatctatga aactctttta gtttacgaat agcgttctta
      241 tggttcatct aatacctcta aagcttctct atgtaattta aaaacggtat tccttgaata
      301 acctagttta tcaggtatct catcccatga taagtcatcc acgtatctag ctttgataac
      361 ggcaatctgt ctctcatctt gcaatgtggc aatcatggct agtctcttat cacgttcctt
      421 agctaaatat aaaagttgtt tagctgtacc tttttcgatg ccatttagta actcaggatt
      481 acgaaaagca ttcattagct tgatgtcctt gtctcgttgt tcctcaaata aggtcattaa
      541 agcaaaaagt ggttt
//
LOCUS       DAUVUY010000202          536 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.205, whole genome shotgun sequence.
ACCESSION   DAUVUY010000202 DAUVUY010000000
VERSION     DAUVUY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.205"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..>536)
                     /locus_tag="VOX58_002014"
     CDS             complement(<1..>536)
                     /locus_tag="VOX58_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HES5879193.1"
                     /translation="DVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSV
                     FIVIDAVKTYAKRYAKLARELAETASPERQAELLEIARICDKVPYEPAATFAEAVQSV
                     WFIQCILQIESNGHSLSY"
BASE COUNT          140 a          123 c          112 g          161 t
ORIGIN      
        1 ataagatagg gaatgcccgt ttgattcaat ttgcaagata cactgaatga accagacgga
       61 ttgcacagct tctgcaaacg ttgctgctgg ttcataaggt actttgtcac aaatgcgtgc
      121 gatttctaac aattctgctt gacgttctgg actagcggtt tctgctagtt ctctagccaa
      181 cttggcatag cgtttagcat aagttttgac cgcatcaatg acaataaaaa ccgaatcgta
      241 aaaatggtat ttgtcaatat tttcaggaat agtcaaatca agcgcagctt tggcagctcg
      301 tgcccgctct tcaaatccct tcaagccgtg ttccaagagt ttttggtaat tgaccgctaa
      361 atgggcatct cccgaattca tcttgccttc catgccaaag aaacctgttt ccatgtacac
      421 ttgcacttct tctggtagca agactccgca gcgagcacgt aagttattgt tttcccagaa
      481 tggggcaata tctcggagct gttgcttggt ttcttcggta atgtagaaca catccc
//
LOCUS       DAUVUY010000203          534 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.206, whole genome shotgun sequence.
ACCESSION   DAUVUY010000203 DAUVUY010000000
VERSION     DAUVUY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.206"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>534
                     /locus_tag="VOX58_002015"
     CDS             <1..>534
                     /locus_tag="VOX58_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5879194.1"
                     /translation="FSFTRNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFVVSLLK
                     ENKKRLYRPLSNIIYPKFNYRIATKNKLLTVLGGLLTITVSVAGMMVMLYAYSLNGIE
                     RLTPSAIEYNVKSENGRVNVTTILENDQVSLVDVGLLRLNTIPEVTITDSGQTIPYFD
                     IINYSDYKELMKAQGRTN"
BASE COUNT          174 a           85 c           99 g          176 t
ORIGIN      
        1 ttttcattca cacgaaatat gctattaagc atagggatgg taccggtttc tctattgata
       61 attgtcttag ttgttttggg aacagtgttt accatcagat atggattggc ttttgtagtt
      121 tcattgttaa aagaaaataa aaaaaggtta taccgtcctc tctctaatat catctatcct
      181 aaatttaact atcgtattgc aacaaaaaat aaattattaa cagtcttggg aggtctttta
      241 acaataactg tttcagttgc cggaatgatg gtaatgctct atgcttattc tcttaatggg
      301 atagagaggt tgactccatc tgccatagaa tataatgtta aatcagaaaa cggtcgcgtt
      361 aatgttacaa ctattttaga gaacgaccag gtgagcttgg ttgatgttgg cctgttgcga
      421 ttgaatacta tcccagaagt aactatcaca gactctgggc aaacaatacc ttattttgat
      481 ataattaact acagtgacta caaagagtta atgaaagctc aaggcagaac aaat
//
LOCUS       DAUVUY010000204          521 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.207, whole genome shotgun sequence.
ACCESSION   DAUVUY010000204 DAUVUY010000000
VERSION     DAUVUY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.207"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(108..521)
                     /locus_tag="VOX58_002016"
                     /pseudo
     CDS             complement(108..521)
                     /locus_tag="VOX58_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572446.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          152 a          102 c           96 g          171 t
ORIGIN      
        1 agctatcaag tgtgtcaaaa catactccga atcctttatg aatacgagct ccgaacttaa
       61 tattatagtc ttcgaaaata cttacaagac atcgttctaa ggcttcttca tttggaaaaa
      121 ccaccctcct tcttgctttg tcgcttgatt tctttattga gtgattcaat caagtttgta
      181 gagtaaatac tagaccaaat gtaatgtgga aattgataga aagttaggag attttccgtt
      241 gtttcaagtg atgtcataac ctttttgtaa cgaggcttcc actcagaaac aagtggtgac
      301 catacatgcg cttaataatg tcactgattt cgcgtgtcgt aacgccagtc tgatagagtt
      361 tgatcactat ctcttccaag tggtcctctc gtctagcata agatggtaat aaaactggtg
      421 aaaactcgcc gtttcgatct cttgagatga ttaaattgac taagccatac tttgtttcaa
      481 actgacgaga ataagtccca ttacggctat tacctgtgtt g
//
LOCUS       DAUVUY010000205          511 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.208, whole genome shotgun sequence.
ACCESSION   DAUVUY010000205 DAUVUY010000000
VERSION     DAUVUY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.208"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>511
                     /locus_tag="VOX58_002017"
     CDS             <1..>511
                     /locus_tag="VOX58_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element IS1548 family transposase"
                     /protein_id="HES5879195.1"
                     /translation="VQFEQSLTSLDAVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGG
                     HHLSLGQVAVEEKSNEIVAIPQLLRTIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCL
                     AVKGNQETLYDDIALYFSDVNLLEELQENAQYYQTVEKSRGQIEVREYWVSSDIKWLC
                     QNHPKWHKLR"
BASE COUNT          169 a           82 c          118 g          142 t
ORIGIN      
        1 aagttcaatt tgagcaatca ttgacaagct tagatgccgt tcatcaactg atttcagtgg
       61 acggtaaaac gattcgaggc aatcgaggta aaaatcagaa gcctgttcat attgtaacgg
      121 cttatgatgg gggtcatcat cttagtttgg gacaggtagc ggttgaggag aaaagtaatg
      181 aaattgttgc cattcctcag ttattgcgga caattgatat ccgtaaaagc attgtaacga
      241 tagacgcaat gggcacgcag acggctatcg ttgatacgat tataaaaggt aaagcagact
      301 attgcttagc cgtcaaagga aatcaagaaa cactttatga tgatattgct ctttatttta
      361 gtgatgtcaa cttattggaa gaactccaag aaaatgcaca gtattatcag actgttgaaa
      421 aatctagggg acagattgaa gttagagaat actgggtgtc ttccgatatc aaatggttgt
      481 gtcaaaacca tcccaaatgg cataagttac g
//
LOCUS       DAUVUY010000206          509 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.209, whole genome shotgun sequence.
ACCESSION   DAUVUY010000206 DAUVUY010000000
VERSION     DAUVUY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.209"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
BASE COUNT          168 a          105 c           97 g          139 t
ORIGIN      
        1 gctgatagct agtaggctca tcagccaaaa cttttttgga gtaacgcttt ggtctcagac
       61 ggcgccagag ggttgagaga ggggtgagaa aaaaacgatg ggtcatcttg gcctccttgt
      121 ctaatgatag tcacgttaac agacaaaagt gttaataatg tcattttaag gagaaaaaga
      181 ttaaaaatca aggtcacaaa cttttctttg ttaaaaaaac gcgaccctaa taaagaacct
      241 aaatctccct cgcacttgag cttctccttg cgcagctatc ttgcgcacac agttgcctcc
      301 aaactaatga tagcgatacc attattgtcc tataggctat cgcgacaacc ccttttttcg
      361 cctcaatgag gctgatacat ctgtcatgac agaaaaaaga cccttgtcta tctaagggac
      421 cattaatttt tctctcacca aaaaactgat tttagaaacg aagaagcata gtgtataata
      481 aagttcggaa caattatgac ataataatg
//
LOCUS       DAUVUY010000207          503 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.210, whole genome shotgun sequence.
ACCESSION   DAUVUY010000207 DAUVUY010000000
VERSION     DAUVUY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.210"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>503
                     /locus_tag="VOX58_002018"
     CDS             <1..>503
                     /locus_tag="VOX58_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HES5879196.1"
                     /translation="NKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVG
                     FDSIVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSI
                     WFVYNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMI
                     LSLIPWE"
BASE COUNT          133 a           85 c          116 g          169 t
ORIGIN      
        1 aacaaaggta gagaaaaaat gctgattgct attttaatcc ctttgtttgc tctaggtgga
       61 acgacctatg gtatggggga agaaaccatg gccttttacc cacttttaat tcctgttatg
      121 attgcggttg gttttgacag tattgttgct gtagccatta ttttgattgg ttctcaaatt
      181 gggtgtctag cctccactat taacccattt gcaactggtg tagcagctga tgctgctggt
      241 gtcagcattg cagatggaat gatttggcgt gttatccaat gggttattct tgtcggcatg
      301 tctatttggt ttgtttacaa ttacgctagt aagattgaag aagacccaag taaatcattg
      361 gttgcagaca aggaagaaga gcataaggaa ctcttccagt tgcaaaattc tggagaagac
      421 ttaaacaagc gccaacgaaa cgttttgacc atttttactt tgacctttgt cattatgatt
      481 cttagtttga ttccgtggga aga
//
LOCUS       DAUVUY010000208          494 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.211, whole genome shotgun sequence.
ACCESSION   DAUVUY010000208 DAUVUY010000000
VERSION     DAUVUY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.211"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..211)
                     /locus_tag="VOX58_002019"
     CDS             complement(<1..211)
                     /locus_tag="VOX58_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HES5879197.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQLASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDI"
BASE COUNT          182 a           92 c           67 g          153 t
ORIGIN      
        1 aaatatccac tgacaagtgt ttgttcttca ccaaatccaa tatacccgtt tttgcagacg
       61 catatcctcc aatcaagaag gctgtaatga aaatagcgga agccagttgt ggaaatgagt
      121 gtaaaaaggc taggccaatt attattaata ccaaacacgc taaagtctcc atcaaatgaa
      181 gatggtctgc catccattgt ctgattgtca tttttagttc cctcacaatt aaaagttatt
      241 caatctttaa gatatattaa ttataataat tctaaataaa taagtcaagt aatttagaaa
      301 cattctaaat aaactttttt aaacttacct tttgctttca aaaatcctaa cttcaactat
      361 ttgcaaatat gaaaaaaact ccccctacct gcggaaatga tatactaata gaaggatgac
      421 tgaccattat agagtgcatg ctttagaact tggcttaact gaagagaaaa ctactaactt
      481 tatgccccaa atat
//
LOCUS       DAUVUY010000209          478 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.212, whole genome shotgun sequence.
ACCESSION   DAUVUY010000209 DAUVUY010000000
VERSION     DAUVUY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.212"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>478
                     /locus_tag="VOX58_002020"
     CDS             <1..>478
                     /locus_tag="VOX58_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HES5879198.1"
                     /translation="GEKGVTIGNYGLNEYEILPDTNTIAITLLRSVGEMGDWGYFPTP
                     EAQCLGKHSLSYSFESITKQTQFASYWRAQEGQVPVITTQTNQHEGTLAAEYSYLTGA
                     DDQVALTAFKRRLADNALITRNYNLSNDKTCDLSLSLPNYNAKVTNLLEKDSKQSIP"
BASE COUNT          154 a          122 c           93 g          109 t
ORIGIN      
        1 ggtgaaaagg gggtcactat tggtaactat ggcctcaacg aatatgagat cttaccagat
       61 accaacacca ttgccatcac tctcttacgt tctgttggtg aaatggggga ctggggttac
      121 ttcccaacac ctgaagccca gtgtcttggc aaacacagcc tttcttatag ttttgaaagc
      181 atcactaagc aaacacaatt tgccagctac tggcgagctc aagaaggcca agtccctgtt
      241 attaccacac aaacaaacca acacgaggga acattagccg cagaatatag ctatttgacg
      301 ggtgcagacg atcaagtcgc tctaacagct ttcaaacgtc gcttagcaga caatgccctt
      361 atcacgcgca actataatct ctcaaacgat aaaacttgtg accttagcct aagcctgcca
      421 aactacaatg ccaaggtcac taatttgtta gaaaaagaca gcaagcaaag cataccca
//
LOCUS       DAUVUY010000210          419 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.213, whole genome shotgun sequence.
ACCESSION   DAUVUY010000210 DAUVUY010000000
VERSION     DAUVUY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.213"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            127..419
                     /locus_tag="VOX58_002021"
                     /pseudo
     CDS             127..419
                     /locus_tag="VOX58_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
BASE COUNT          146 a           76 c           95 g          102 t
ORIGIN      
        1 ataactcagg acaagatgag catggcagct aaaaagaaag ataagatgtc aacatcaaag
       61 gacaagtcca tgatggcaga taaatcatct gataagaaaa tgaccaatga tggtcctatg
      121 gcgcctgatt tcgaactcaa aggcatcgat ggtaagacct atcgcttatc agagttcaaa
      181 ggtaaaaaag tttatttgaa attttgggcc tcttggtgtt caatctgtct atcaaccttg
      241 gcagataccg aagacctggc taaaatgtca gataaagact atgtggtcct aacagttgtc
      301 tcgccaggtc atcaagggga aaaatcagaa gcagacttta aaaaatggtt ccaaggaaca
      361 gactataagg acttaccagt cttgttagat ccggatggca agctattgga agcttacgg
//
LOCUS       DAUVUY010000211          411 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.214, whole genome shotgun sequence.
ACCESSION   DAUVUY010000211 DAUVUY010000000
VERSION     DAUVUY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.214"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(62..409)
                     /locus_tag="VOX58_002022"
     CDS             complement(62..409)
                     /locus_tag="VOX58_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HES5879199.1"
                     /translation="MTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRSSCPYCRRFA
                     PKLAQVATDNQKDVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQHQRAVCDSS
                     LTPDDILAFLTRE"
BASE COUNT          123 a           94 c           86 g          108 t
ORIGIN      
        1 ggtatagcaa gactagacgt tacctgattt cttctttaaa cgagacgcta agcgcagtgc
       61 tttactcacg agttaaaaaa gctaggatgt catctggtgt caaacttgaa tcacatacag
      121 ccctttggtg ctgatcgtaa ctaaccagca aagctggaac tgtcactaat tggtagttct
      181 cacgaaaggc agcaagctca gcagcgtcag cagcgttttc actatccaca aaatagacgt
      241 ctttttgatt atctgttgcc acttgtgcca acttaggagc aaagcggcga caataaggac
      301 agctcgaacg tcctaaaaag acaatcatgt ctttgccact agcgatagct gatgtgactt
      361 ctgcaacact cgacggtata aaattcgcaa cgatttcttc aaatgtcatg g
//
LOCUS       DAUVUY010000212          409 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.216, whole genome shotgun sequence.
ACCESSION   DAUVUY010000212 DAUVUY010000000
VERSION     DAUVUY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.216"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            229..354
                     /locus_tag="VOX58_002023"
     CDS             229..354
                     /locus_tag="VOX58_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="HES5879200.1"
                     /translation="MNNKKTKNNFSTLSESELLKVIGGDIFKLVIDHISMKARKK"
BASE COUNT          173 a           54 c           50 g          132 t
ORIGIN      
        1 gtaatctctt caaggttact gcactctcca aactttctaa taaagacagt gacgtcgtac
       61 agtatggatt gcaaggacgt gtaacaagtg tcattacacg gaaaagctat ttcaattact
      121 ataaagataa gatactcaac agttttaatt aaatataata aaattccaat ttattccaga
      181 aattattaaa aactaaaatt tcatataatt ttattaggag atattacaat gaacaataaa
      241 aaaacaaaaa ataacttttc aacattatct gaatctgaat tacttaaagt gattggtgga
      301 gatattttta aacttgtcat agatcatatt agtatgaaag cgagaaaaaa gtaagttcct
      361 tctaaaataa ttttaaaata taaactagta attcatcatt cataaaagc
//
LOCUS       DAUVUY010000213          401 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.217, whole genome shotgun sequence.
ACCESSION   DAUVUY010000213 DAUVUY010000000
VERSION     DAUVUY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.217"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..224
                     /locus_tag="VOX58_002024"
     CDS             <1..224
                     /locus_tag="VOX58_002024"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HES5879201.1"
                     /translation="VKMVSSTLEIHPNSLYRWIQEYEKYGESAFPGHGSALRHAQFET
                     KKLEKEHKLLQEELALLKKFQVFLKPNRK"
BASE COUNT          142 a           69 c           75 g          115 t
ORIGIN      
        1 ctgtaaaaat ggttagttca actttagaaa ttcatcccaa tagtctttat cgatggattc
       61 aagaatatga aaaatatgga gaaagtgcgt tcccaggaca tgggagcgca cttcgtcatg
      121 ctcaatttga gacaaaaaaa cttgaaaaag agcacaaact gctacaggag gaattagctc
      181 ttctaaaaaa gttccaggtc tttttgaagc caaaccggaa ataaaatttc agtttctgaa
      241 ggaaaatagt gttagcttga atagtcatca cacctgtcaa actcttcaag tctcccgttc
      301 aggtttctat gcttatttaa aacgtcgtct atcttcaaag catgttgaga atgaagcttt
      361 gaaggaaatg attaaagaca ttttctatga acataaagag c
//
LOCUS       DAUVUY010000214          390 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.218, whole genome shotgun sequence.
ACCESSION   DAUVUY010000214 DAUVUY010000000
VERSION     DAUVUY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.218"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..376)
                     /locus_tag="VOX58_002025"
     CDS             complement(<1..376)
                     /locus_tag="VOX58_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HES5879202.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAYLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPA"
BASE COUNT          121 a          105 c           84 g           80 t
ORIGIN      
        1 tagcaggacc tgtcgcaaat gtccctaaaa tatcagcctg actctcagca gccagataat
       61 gtggcctgaa ctgaaggaat accagagttg aaccaaccat agctcccaat agttgggcaa
      121 ggctataggc aatggctgtt gaccaggcaa tggttccact catggcaaaa gcaaggctaa
      181 cagctggatt gaggtaggca ggggcaactt tgccactgat aaagactgcc acagcaactg
      241 caattcccca gccagtcgca atcacgatcc agccagaagc atgggttttc gttttgggca
      301 agacaacccc tgctaccaca ccatttccta gtaaaaccaa taaggctgta cccaaaaatt
      361 cgccaaagat atccatatta accctcctga
//
LOCUS       DAUVUY010000215          389 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.219, whole genome shotgun sequence.
ACCESSION   DAUVUY010000215 DAUVUY010000000
VERSION     DAUVUY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.219"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>389
                     /locus_tag="VOX58_002026"
     CDS             <1..>389
                     /locus_tag="VOX58_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879203.1"
                     /translation="IKDHDYKITFEQGQIASGITVDYRIGSAFNKTTDDSFKISDESK
                     YASNVKIEGEEQGFKQREQGDKTISFRTLKEGPMSLVLLSKVEKKPQGDLDVEFKNFK
                     IIDVTNPSQLDKGVAYVGNKNVELTLKS"
BASE COUNT          156 a           47 c           73 g          113 t
ORIGIN      
        1 ataaaagatc atgactataa gataactttt gagcaaggtc agattgcgtc gggaataaca
       61 gttgattatc gtataggtag tgcttttaat aaaactacag atgattcttt caaaatttca
      121 gatgagagta aatatgcttc taacgtaaaa atagaaggtg aggaacaagg ttttaaacaa
      181 agggaacaag gagataaaac aatatctttt agaactttaa aagaaggtcc tatgtctctt
      241 gtattgttat ctaaagttga gaaaaaacct caaggagatc ttgatgtcga gtttaaaaac
      301 ttcaaaatta ttgatgttac aaacccaagc caattagata aaggagttgc ttacgttgga
      361 aacaaaaatg tagaattgac attaaaatc
//
LOCUS       DAUVUY010000216          382 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.220, whole genome shotgun sequence.
ACCESSION   DAUVUY010000216 DAUVUY010000000
VERSION     DAUVUY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.220"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>382
                     /locus_tag="VOX58_002027"
     CDS             <1..>382
                     /locus_tag="VOX58_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HES5879204.1"
                     /translation="FEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILRDFNESL
                     GVNDRVALATAETVMRQRITQKHMVNGVTFHNPETVYIESDVEIAPDVLIEGNVTLKG
                     RTHIGSGTVLTNGTYIVDSEIGQGS"
BASE COUNT          120 a           66 c           91 g          105 t
ORIGIN      
        1 tttgaagcgc tcaaatgcat cactaccaac aatgctcaag gagagtatta cctgactgat
       61 gttgtggcta tttttagagc aaacaaagaa aaggtaggag cctatatcct gagagacttc
      121 aatgaaagtc ttggtgttaa tgatcgtgtg gccttagcaa cagctgaaac agtgatgcgt
      181 cagcgcatca cccaaaaaca tatggtcaat ggggtgacct ttcacaatcc tgaaactgtt
      241 tatattgaaa gcgatgttga gattgcaccg gatgttttaa ttgaaggaaa tgtgacctta
      301 aaagggcgta cacatatcgg ctcaggaact gtcttgacga atggtactta tattgtggat
      361 tctgaaattg gtcaaggaag ta
//
LOCUS       DAUVUY010000217          380 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.221, whole genome shotgun sequence.
ACCESSION   DAUVUY010000217 DAUVUY010000000
VERSION     DAUVUY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.221"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            44..>380
                     /locus_tag="VOX58_002028"
     CDS             44..>380
                     /locus_tag="VOX58_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HES5879205.1"
                     /translation="MTELLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAKKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRL"
BASE COUNT          119 a           63 c           88 g          110 t
ORIGIN      
        1 acaactcttt tttgacatgc tatactgata gcgggagtaa atcatgacag aattattata
       61 caaaaagata tatcatttta ttaaagagca gatagcatca ggtcgtttgc aaattggtga
      121 ccgcctacct actgaaaaag aattgtcaga acagttctcg gttagccgta tcacttctaa
      181 gcgagcgctt gtagaactgg agcaagaagg cttgattacg agaagtcgtg ggaaaggaag
      241 cttcgtagct aaaaagcaag tgacgtcccc tgaagcgaat aaggatttat tgttgattct
      301 tccttttgct tctgattatg aattgggaga ttatgctaag ggaattatga cctccattgc
      361 tgaaacaggg tatcggttga
//
LOCUS       DAUVUY010000218          368 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.222, whole genome shotgun sequence.
ACCESSION   DAUVUY010000218 DAUVUY010000000
VERSION     DAUVUY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.222"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
BASE COUNT          157 a           51 c           48 g          112 t
ORIGIN      
        1 tctctctatt gctatcaatc aacaaataca aagcaaatta aactcactaa tcaaaaatac
       61 ttgtggaaag agcaactaac tttttttcgt accattaata taaaaaatag ggataaataa
      121 gtattataaa atctctaaaa agcatggttt tgaaattaaa aaaatataat tttagactac
      181 ttaaaaattg gaaactacta gctgtcactt cgaagtagta ttatcctaga ctgtttcatt
      241 aagactaagg atataattta tcaaataaaa gtttattcgc ctcaactaag aaaggagggg
      301 agtttcccaa aatgtagcaa aaaataaaca gaaagttaaa ctaaagcttg aatgcttaag
      361 ttctatgt
//
LOCUS       DAUVUY010000219          366 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.223, whole genome shotgun sequence.
ACCESSION   DAUVUY010000219 DAUVUY010000000
VERSION     DAUVUY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.223"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..101
                     /locus_tag="VOX58_002029"
     CDS             <1..101
                     /locus_tag="VOX58_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="role in replication"
                     /protein_id="HES5879206.1"
                     /translation="YVLGYPGDFLDVTNHRVISAKKFHKEYQIIDG"
BASE COUNT          121 a           52 c           69 g          124 t
ORIGIN      
        1 tgtatgtcct tggttatcca ggtgatttct tggatgtgac caaccatcga gttatttctg
       61 ccaaaaaatt ccataaagaa tatcagatta ttgatggata gaaaaagtag actatgaact
      121 tttatgactg gtttaatttt gagggtttac aacaaaaagg agctctttgg aagattgtag
      181 gaagtatttt ctttgtcggt tttcatttat tcatcatcta tttggtacaa agataattag
      241 ttcaacttta aaaattcatc ccaatagtct ttatcaatgg attcaagaat atgaaaaata
      301 tggagaaagt gcgttcccag gacatgggag cgcacttcgt catgctcaat ttgagataaa
      361 aaaact
//
LOCUS       DAUVUY010000220          364 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.224, whole genome shotgun sequence.
ACCESSION   DAUVUY010000220 DAUVUY010000000
VERSION     DAUVUY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.224"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
BASE COUNT          116 a           92 c           63 g           93 t
ORIGIN      
        1 tcaccaccat gcttaggacg ggcaacactt tcttttctgg catacatgct cctttctcaa
       61 cacacgggtc actaaacccc aaaaaggccc ccctatcaga ggtggcctga ctactaaagg
      121 gactatccca attatagcag attagtgggg gaatggcttg agctaagcaa agtatcctca
      181 agctcttcaa taagttacaa taactaaatt aggacaacaa atacgtcatt tactaagaat
      241 ttgcaccacc aaacaaagca ccaaatgcat atgttaagta gtaccaagcc atgtctagta
      301 gtgctgtgat tggatgacta tcgtaccatc cttctccata acctgtacta tagccatcac
      361 tgta
//
LOCUS       DAUVUY010000221          361 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.225, whole genome shotgun sequence.
ACCESSION   DAUVUY010000221 DAUVUY010000000
VERSION     DAUVUY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.225"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..203)
                     /gene="gatC"
                     /locus_tag="VOX58_002030"
     CDS             complement(<1..203)
                     /gene="gatC"
                     /locus_tag="VOX58_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HES5879207.1"
                     /translation="MKISEEEVRHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDVA"
BASE COUNT          100 a           80 c           58 g          123 t
ORIGIN      
        1 tcagcgacat cttgacgcat cacatttttc ttatctgcca tggttgttgt aatggccaca
       61 ccttcggtat caacttcgtt cagcaattca accatgtcaa cgattttaga tagggtcgtt
      121 gcaaaggttg ttgtctcact ttctgagaat gataattttg atagcttcgc gacatggcga
      181 acttcttctt cagaaatttt catgatttct cccatccaag atgacaagaa gtattccagt
      241 tccaaagaac agctctgcta ttctctaaag gtagcggaaa aacttctcac atcattttta
      301 catagtctca attattatat cacaattgtt cccatttacc ataggtgact aatgcaattc
      361 t
//
LOCUS       DAUVUY010000222          356 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.226, whole genome shotgun sequence.
ACCESSION   DAUVUY010000222 DAUVUY010000000
VERSION     DAUVUY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.226"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
BASE COUNT          154 a           41 c           54 g          107 t
ORIGIN      
        1 gtgacaataa tatcacagat aaaaaccaaa aacagcaaaa ataattaaat tgaatataaa
       61 aaagttaatt aagtgagtga taataaagaa atgaagttca aaaaatataa ataaaataaa
      121 ataccagaac tgctaaaagc agtgaatgac ataaatgtcg ctaaaaaaat agatagaaga
      181 gtctaaaatt tcagttttag gtcacgtttt atgaatgtaa atcagaatga tgaccttaag
      241 ttgtgatttg cgcgtcattg accgctttcc tcgttttaat gaaagaattt gaaagatatt
      301 ttatggtata cttttcctta atatggttca tacggacttg ataaatctat tcacaa
//
LOCUS       DAUVUY010000223          356 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.227, whole genome shotgun sequence.
ACCESSION   DAUVUY010000223 DAUVUY010000000
VERSION     DAUVUY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.227"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>356
                     /locus_tag="VOX58_002031"
     CDS             <1..>356
                     /locus_tag="VOX58_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HES5879208.1"
                     /translation="DPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVKNALISYGIDD
                     KVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYLEKSLYESLFP
                     KDDGLGKFVEVMGNPF"
BASE COUNT          130 a           69 c           65 g           92 t
ORIGIN      
        1 tgaccctaaa tttccagaca agtcaaatgc taaaaaacca gactatcttc cttttatggc
       61 tgaagaagag cttagcaaaa tacagcaagt caaaggcgtc aaaaatgccc tgatctcata
      121 tggtattgat gacaaagtct accatctagg ccaaaaatca tctgcaaaga tttctgccat
      181 cacaaaaaac gtagctgaag tgagacgcat gacatttatt aaaggcagtg acttttcaga
      241 caaagacttt attgaccaaa aacaggtgat ttaccttgaa aaaagcttgt atgagtcact
      301 atttccaaaa gacgatggtc ttggaaaatt tgtcgaagtg atgggaaatc ccttca
//
LOCUS       DAUVUY010000224          348 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.228, whole genome shotgun sequence.
ACCESSION   DAUVUY010000224 DAUVUY010000000
VERSION     DAUVUY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.228"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(76..345)
                     /locus_tag="VOX58_002032"
     CDS             complement(76..345)
                     /locus_tag="VOX58_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HES5879209.1"
                     /translation="MEGNFGEIFKTFRESKGLRLKDVAKAGISTSQLSRFEKGETDLT
                     ISTFMLILDESNMLIDEFMYAVHDFHRDDLNELLSKSEAFRNNSR"
BASE COUNT          107 a           78 c           52 g          111 t
ORIGIN      
        1 caaaagatat ccgtgaacat ctccttctta tcagttgatg ctagttgagc atgaagtaac
       61 tgcttcaagt catcactatc ttgagttgtt acgaaatgcc tcactttttg ataagagttc
      121 atttaagtcg tcacgatgaa aatcatggac agcatacata aactcatcaa tgagcatgtt
      181 actttcatcc aaaatcagca taaatgtcga tatggttaaa tccgtctccc ctttttcaaa
      241 acgcgacagc tgagaggttg atataccagc ctttgcgaca tcttttaatc gtaatccctt
      301 tgattctcta aaggttttaa aaatctctcc aaaatttccc tccaacac
//
LOCUS       DAUVUY010000225          347 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.229, whole genome shotgun sequence.
ACCESSION   DAUVUY010000225 DAUVUY010000000
VERSION     DAUVUY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.229"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(<1..>347)
                     /locus_tag="VOX58_002033"
     CDS             complement(<1..>347)
                     /locus_tag="VOX58_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HES5879210.1"
                     /translation="FASQAWYDTITRDFVAGECVWTGFDYLGEPTPWNKIDSGVVGLW
                     PSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHLLPVWQKDQLCFDEQGLVEVVV
                     YSNAASVQLMFED"
BASE COUNT          102 a           94 c           70 g           81 t
ORIGIN      
        1 tcatcttcaa acatcagttg cacactggca gcattgctat aaaccacaac ctccaccaat
       61 ccctgctcat caaaacaaag ctggtccttc tgccagactg gtaacaggtg caaggtggtc
      121 tgcccctgcg cccactgact ttggtaaaaa taatagctat cttttggaaa gcctgccgtg
      181 tctaaaatgc caaagtaggc atttttagga gaaggccaaa ggcctacaac accgctgtcg
      241 atcttgttcc agggtgtagg ttctcctaaa taatcaaagc ctgtccaaac gcattcgcca
      301 gcaacaaaat cacgggtaat ggtatcatac caagcctggc tagcgaa
//
LOCUS       DAUVUY010000226          343 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.230, whole genome shotgun sequence.
ACCESSION   DAUVUY010000226 DAUVUY010000000
VERSION     DAUVUY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.230"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(132..>343)
                     /locus_tag="VOX58_002034"
     CDS             complement(132..>343)
                     /locus_tag="VOX58_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HES5879211.1"
                     /translation="ADKYGWDLALARVLAALLIYGTGFGVLLYILLAIFLPYKEDLLE
                     ERYGRGPRRRKDADVLNEEEDGWFW"
BASE COUNT          118 a           82 c           52 g           91 t
ORIGIN      
        1 taagtcaaaa agcaaaaagc ctcatcatag ctgataagac ccctcgctta cttacatatc
       61 aaaacatttt attatcatca gttaatcaca tggttgctct tacgtgatga attatcattt
      121 taactgattg tttaccagaa ccagccatct tcttcttcat ttaagacatc agcgtcttta
      181 cgacgacgag gacctcggcc ataacgttct tcaagcaagt cttccttgta aggtaagaag
      241 atggccaaca aaatataaag caaaactcca aaaccagttc cgtaaataag aagagccgct
      301 aagacacgcg ctagagcaag atcccaacca tacttatctg cta
//
LOCUS       DAUVUY010000227          338 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.232, whole genome shotgun sequence.
ACCESSION   DAUVUY010000227 DAUVUY010000000
VERSION     DAUVUY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.232"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>338
                     /locus_tag="VOX58_002035"
     CDS             <1..>338
                     /locus_tag="VOX58_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HES5879212.1"
                     /translation="ENITEPLAAMAYEVTSTYPHDQLRYAWKTLMQNHPHDSICGCSV
                     DSVHREMITHFEKAYEVGHYLAKEAAKQIADAIDTRDFPMDSQPFVLFNTSGHSKTSV
                     AELSLTWKKY"
BASE COUNT          106 a           82 c           66 g           84 t
ORIGIN      
        1 tcgaaaacat caccgaaccc ttagcagcaa tggcttatga ggtaacaagt acctaccctc
       61 acgaccaact gcgttacgct tggaaaaccc tcatgcaaaa tcaccctcat gattctatct
      121 gtggttgtag tgttgatagc gttcatcggg aaatgataac gcactttgaa aaagcctatg
      181 aagtcggaca ctatttagca aaagaagctg ctaagcaaat tgctgacgcc attgatacca
      241 gggattttcc aatggatagc caacccttcg tcttatttaa taccagcggc cattccaaaa
      301 caagtgttgc tgagctcagc ctgacctgga aaaaatat
//
LOCUS       DAUVUY010000228          330 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.233, whole genome shotgun sequence.
ACCESSION   DAUVUY010000228 DAUVUY010000000
VERSION     DAUVUY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.233"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            complement(52..133)
                     /locus_tag="VOX58_002036"
     tRNA            complement(52..133)
                     /locus_tag="VOX58_002036"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Leu,seq:tag)
     gene            complement(139..211)
                     /locus_tag="VOX58_002037"
     tRNA            complement(139..211)
                     /locus_tag="VOX58_002037"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Lys,seq:ttt)
     gene            complement(243..315)
                     /locus_tag="VOX58_002038"
     tRNA            complement(243..315)
                     /locus_tag="VOX58_002038"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(280..282),aa:Asp,seq:gtc)
BASE COUNT           87 a           94 c           68 g           81 t
ORIGIN      
        1 tgattacaaa tcagatgctc taccaactga gctaagccgg ctaatctttt atgcgggtta
       61 agggacttga acccccacgc cgttaagcgc cagatcctaa atctggtgcg tctgccaatt
      121 ccgccaaacc cgcttttatg acccgtactg ggctcgaacc agtgacccat tgattaaaag
      181 tcaattgctc taccaactga gctaacgagt ctttccttca aaaaatatga ctactctttg
      241 aacggtcccg acgggaatcg aacccgcgat cttcgccgtg acagggcgac gtgataaccg
      301 ctacactacg ggacctgtat tttccaatac
//
LOCUS       DAUVUY010000229          293 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.235, whole genome shotgun sequence.
ACCESSION   DAUVUY010000229 DAUVUY010000000
VERSION     DAUVUY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.235"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>293
                     /locus_tag="VOX58_002039"
     CDS             <1..>293
                     /locus_tag="VOX58_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HES5879213.1"
                     /translation="QRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKN
                     ICGKANEYDVTKQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKK"
BASE COUNT           92 a           46 c           65 g           90 t
ORIGIN      
        1 caacgatttg acttaggcaa tttaccggct aatcctgttt tagtgaaatc cattgtaaca
       61 ggagacttgt cacgggctat tgctagccat tatggtattg aaaccgttga aacattgact
      121 ggttttaaga atatttgtgg caaggcaaac gaatatgatg tgacgaaaca aaaaaactac
      181 ctgtttggtt atgaagaaag tattggtttt tgctatggca cttttgttcg tgacaaagac
      241 gctgttagcg cctctatgat gattgtagaa atggcagctt actacaagaa aaa
//
LOCUS       DAUVUY010000230          282 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.denovo.237, whole genome shotgun sequence.
ACCESSION   DAUVUY010000230 DAUVUY010000000
VERSION     DAUVUY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.denovo.237"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            115..>282
                     /locus_tag="VOX58_002040"
     CDS             115..>282
                     /locus_tag="VOX58_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HES5879214.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAAS"
BASE COUNT           87 a           47 c           46 g          102 t
ORIGIN      
        1 tatcatttta tcaaaaaatc cataatttgt catgctttcc caaaaaagta tagtcaaaat
       61 aatggttagc tgtctcattt tgttttatgg tataatgaaa taaaggagtc tgttatgtca
      121 attaaagcaa tcaaaggcca ggctagagac acgttaaaaa atctctctgg caagtattta
      181 ctatttttaa ttccaaccct tctctttatg tttcatttcg gtattgagat tcatcaaggt
      241 tacgtcctat ctagtggtat tgaagtctcg ctagcggcta gt
//
LOCUS       DAUVUY010000231         5052 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.guided.102, whole genome shotgun sequence.
ACCESSION   DAUVUY010000231 DAUVUY010000000
VERSION     DAUVUY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.guided.102"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            53..787
                     /locus_tag="VOX58_002041"
     CDS             53..787
                     /locus_tag="VOX58_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HES5879215.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
     gene            complement(1175..1381)
                     /locus_tag="VOX58_002042"
     CDS             complement(1175..1381)
                     /locus_tag="VOX58_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010010243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HES5879216.1"
                     /translation="MEIFDKYYDEHNLEERSQYSGFSKKQLVIEAEHLENALSRVLVY
                     LENNGDDLDFIRTEVMDGLYESRI"
     gene            complement(1368..2033)
                     /locus_tag="VOX58_002043"
     CDS             complement(1368..2033)
                     /locus_tag="VOX58_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HES5879217.1"
                     /translation="MAKEKSRNFTFLLYPDGDGFPSDWEERLEKIGVPIAISPLHDKD
                     KNKNRTLKKPHYHGIYIANNPVTAESVKNKLRAVLSSEDVECKAVAKVQIIYESIESV
                     YLYLTHESKDAIKKNKYRYDKADIKHINNFDIDRYVVVDVETKNQILKILLQIIRAYS
                     IPNVLDLHDFIEENGSSYGIDMNLFLTTIESKSSILRLYFDGAYQRGQRGLKSGEEKN
                     GNI"
     gene            complement(2088..2234)
                     /locus_tag="VOX58_002044"
     CDS             complement(2088..2234)
                     /locus_tag="VOX58_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HES5879218.1"
                     /translation="MAKKRMGISLSEEVAEMLEKQAKEAGLNKSALITTLIVKENKNQ
                     DRKV"
     gene            complement(2950..4452)
                     /locus_tag="VOX58_002045"
     CDS             complement(2950..4452)
                     /locus_tag="VOX58_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HES5879219.1"
                     /translation="MSYLVARMQKMKVGNLGGAYRHNERVFENHSNKDIDTSRSHLNY
                     ELTDRDRSVSYEKQIKDYVNEKKSSKRAIRKDAVLCDEWIITSDKDFFERLDQEQTRA
                     FFETAKNYFAERYGLENIAYASVHLDESTPHMHMGVVPMVDGKLSSKAVFTREELKAI
                     QEELPKYMNEQGFELSRGQLGSDKKHLSVADYKAKIGKEALNKELLGLGAPRYWHKEE
                     DRPATAEEIAGYESLASLFAGEEMKLREATLEERFTWLDSHRNDLKGDLSHLEELVDK
                     KIEEYTRIDSEASERLSELSELNSKVKDREKELRGLESDSERLSDKVVRLEKEHRETT
                     QLLVEQNRNLRKISFQDLDRRRIAEDLHEELEKATPKLFGGSFNFTADFVGRLKTFMS
                     EVVEKLEQVINQNEVLRKALEGMKQAKERAERELLQEEWKTQRLETENQNLRQENKEL
                     KVSKNLLEDIQEVITEKEVSSLNKRLDELREARMASKRGYEPKRSKGRSI"
     gene            4901..4960
                     /gene="ermCL"
                     /locus_tag="VOX58_002046"
     CDS             4901..4960
                     /gene="ermCL"
                     /locus_tag="VOX58_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HES5879220.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
BASE COUNT         1479 a         1127 c          759 g         1687 t
ORIGIN      
        1 cttccttttt tctatttaca caaaatattt tacactctcg gagtgggtta taatgaacaa
       61 aaaaaatata aaagatagtc aaaactttat tacttcaaag catcatataa atgaaatttt
      121 gagaaatgta catttaaata caaatgataa tattattgag attggttcag ggaaaggtca
      181 tttcacgttt gaattagcta aaaggtgtaa ttatgtaacc gccattgaaa tagatcctaa
      241 attatgtagg ataactaaaa acaaacttat tgaatatgag aacttccagg ttatcaataa
      301 agatatttta caatttaagt ttcctaaaaa taagtcatat aagatatatg gaaatatacc
      361 ctacaatata agtacagata taattcgaaa aattgttttt gaaagcacag ctacagaaag
      421 ttatttaata gtggaatatg gatttgctaa aaggttgcta aatacaaatc gttcactagc
      481 actattttta atgacagaag ttgatatatc catattaagt aaaatcccta gagaatactt
      541 tcatccaaaa cctagagtta atagctcgtt aattgtatta aaaagacacc cttcaaaaat
      601 atcactcaaa gatagaaaac aatatgaaaa ttttgttatg aaatgggtta acaaagaata
      661 cataaaacta ttttccaaaa accaatttta tcaagcctta aaatatgcaa gaattgacga
      721 tttaaacaat attagctttg aacaattctt gtctcttttc aatagctata aattatttaa
      781 tagataagtt aaggggtgca taaactgcat cccttaactt atcttatttt gcttaaacaa
      841 tcttggcccg taaactgcat aactcatatg taaatcgctc ctttttaggt ggcacaaatg
      901 tgaggcattt tcactatttc cggcaaccac ttccaagtaa agtataactc actatacttt
      961 acttcgtaaa tgtgcgttct ccgaagggtc ttgcagacgg ccagctcgcc acaaaggaaa
     1021 ggtggcgaga taacaaaaac gccctcgaac cacaaggaca tttttgtcat gtttggacga
     1081 cttcgacaag tcgcccaaag tcattttttc aaaatgacgg tccgaccgct tcgctattgt
     1141 cggactaaaa agctgacccc gaaaggtcag ctttttatat gcgactttca tacagtccat
     1201 ccattacttc tgtgcgaatg aaatctaaat catcaccgtt attttctaga taaactaaaa
     1261 cccttgacaa agcattttct aaatgttccg cttcaatcac caattgcttt ttgctaaatc
     1321 cactatattg actgcgttct tctaggttat gctcatcgta atacttatca aatatttcca
     1381 tttttctctt cccctgactt caaccctcgt tgaccacgct gatacgctcc atcaaaatac
     1441 aaacgcaaga tactcgattt gctttcaatt gtcgtcaaaa acaaattcat atcaatccca
     1501 taacttgaac cattttcctc gatgaaatca tggaggtcaa gcacgtttgg aattgaataa
     1561 gcacgaataa tctggagcaa aattttcaaa atttggtttt tggtttcaac atcaaccaca
     1621 acatagcggt caatatcaaa gttgtttata tgcttaatat ctgccttgtc ataacgatat
     1681 ttgtttttct tgatagcatc cttactttca tgcgtaagat acaaatagac actctcaatt
     1741 gattcgtaaa taatctgcac tttcgctacc gctttgcact ccacatcttc agaactgaga
     1801 acagccctca atttgttttt tactgattca gcggttactg gattatttgc aatataaatc
     1861 ccatgataat gcggtttttt cagcgttcta tttttattct tatctttgtc atgaagcgga
     1921 ctaattgcaa tcggtacgcc gattttttct aaccgttctt cccaatcgct tggaaaccca
     1981 tccccatcag gatagagcaa aaatgtaaaa tttctcgatt tttcttttgc catatgctat
     2041 aattaaccta actcaaaaat accgctgtgt ccccaacaca gcttttttta tacctttctg
     2101 tcttgattct tgttttcttt aacaatcaaa gtcgttatta aagcagattt attcaaaccg
     2161 gcttctttcg cttgtttttc aagcatttcc gctacttctt cagaaagcga aatccccatt
     2221 cgtttttttg ccataattac acctttttct ttttttgaca ttgtaacacg ttttacaaat
     2281 ttttgcaaca ttttttgttt ttttatcatg gatttgctct ctaaacccta ttttttgctc
     2341 tctaaatacg aatttccgaa tttagagagc aacctctaaa gccttgaggc tctaagcctg
     2401 aaccctaatt ttaccccgtt ttggcactcg gcacctaagg ggggtcgtag tagcccccca
     2461 gattttagat ttttctgccg aaaaatcccc ctcaagtttt tttcacgtgc ccattttttg
     2521 acacgtgctt tcccacgtga tacaatgagt ttacgaaaat gtagtgcaca agcacaccga
     2581 agcccctaag gaatgccagt tccctagggg cttttttgtc ggtgttgggc tagcttcagc
     2641 gtttgtcctt gctatctaac cacttgcaaa tgtaatgtgc cgttacttct gccacaatag
     2701 caagtaagac gtcgaaaatg taatgcactc gctccacctc ctttcgcata gacttgcgga
     2761 aggttttgct tcagcattgg ttattatatc atgtccgctt actttattcc atgttctcct
     2821 tgacagcaag taacctgtgg cggtagttct tggtctgccg gttggtcgaa caaagttctg
     2881 accaaaccgc ccgccctaag tctaccttgc cccagcttgc tatcaaggag ctttcacggc
     2941 ataaatcgct cagatactcc gacctttaga acgtttaggt tcatatcctc tcttagaagc
     3001 cattctagct tctctaagct catctaaacg cttattaaga ctactaacct ctttctctgt
     3061 gataacctct tgaatgtctt ctaagaggtt tttagaaacc tttaactctt tgttctcttg
     3121 tctcaaattt tgattctctg tctctagtct ttgagttttc cattcttcct gcaataattc
     3181 tctttccgcc ctctctttag cttgcttcat gccttctaac gctttcctca acacttcgtt
     3241 ttgattgatt acctgttcca gtttctcaac aacttcagac ataaaagtct tgagtcgacc
     3301 aacaaaatct gccgtaaagt taaaactacc accaaacaat ttaggggtcg ctttctctaa
     3361 ctcttcatgc aaatcttctg caattctacg tctatccaaa tcttgaaacg aaatcttccg
     3421 caaattcctg ttttgttcga ccaaaagttg tgtcgtttcc ctatgttcct tttccaaacg
     3481 aaccacttta tcgctcagac gctcagaatc gctctctaag ccccttaatt ctttttctcg
     3541 gtcttttacc ttggaattta actcagatag ctctgagagc ctctcagagg cctcagaatc
     3601 gattctagta tattcttcaa tctttttatc cactaactcc tctaaatggc ttaaatcgcc
     3661 ctttaaatcg tttctatggc tatctagcca tgtaaaacgc tcttccaacg ttgcttcacg
     3721 tagtttcatt tcctctccag caaacaacga ggctaaactt tcataacctg ctatctcttc
     3781 tgcagttgct ggtctatcct cctctttgtg ccaataacga ggagcaccca gccctaataa
     3841 ttctttattc agagcttctt tcccaatttt agccttgtaa tcagctacac taaggtgttt
     3901 cttatcactt cctaattgcc cacgtgacaa ctcaaaaccc tgctcattca tatattttgg
     3961 cagttcttct tgaatagcct ttaattcttc acgggtaaag acagctttag acgacaattt
     4021 tccgtccacc ataggtacta ctcccatatg catgtgaggg gtactctcgt ccaaatgtac
     4081 gctagcataa gcaatatttt ctaagccata acgttcagca aagtaatttt tggcagtctc
     4141 aaaaaatgcc ctggtctgct cctggtctaa cctctcaaaa aagtctttat cagacgtgat
     4201 aatccactca tcacataaaa ccgcatcttt ccgaatagct cgcttagagc tcttcttttc
     4261 attcacgtaa tccttaatct gcttttcata cgacacagag cggtcgcgat ccgtcaactc
     4321 atagttgagg tgcgacctgc tcgtgtctat atctttgttc gagtgattct caaaaacacg
     4381 ttcattgtgc ctataagctc cgccaagatt tccgactttc attttttgca ttcttgctac
     4441 gagataactc atccttcagt tccttcccag gtggtcattc gaatagcgaa tgacaggcaa
     4501 gccgcccagg ctattgcttg cctaaatacc taacatctta gctagtagtt tagcacattt
     4561 tcggcacaac ttccatgtaa agtatacaca ctatacttta tattcatata agtgtgtact
     4621 ctgcgaggct gtcggcagtg ccgaccaaaa ccataaaacc ttttaagacc tttttttttc
     4681 gtaaaaaaag aaacaaaaaa acccgacctc tgccacctca gcaaaggggg gatgctttcg
     4741 ttaccactca aaaataaaaa agatacaaga gaaatctctc gtatctttat tcaacaatag
     4801 ggcccaattg cagtataaat ttaacagtcg ttcatcatgt tattatttat cagagcttgt
     4861 gctataatta ttctaatttc ataaaaagga gaattaatat atgggcattt ttagtatttt
     4921 tgtaatcaac acagttcatt atcaaccaaa caaaaaataa gtggttataa tggattgtta
     4981 atataaacat tcattataac ctcataggag tggggggagc gtcaataatt ttgtgtaaat
     5041 gattcttccc ta
//
LOCUS       DAUVUY010000232         1009 bp    DNA     linear   BCT 08-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain 116
            SAMN27862179-rid17134413.guided.181, whole genome shotgun sequence.
ACCESSION   DAUVUY010000232 DAUVUY010000000
VERSION     DAUVUY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27862179
            Sequence Read Archive: SRR18933625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001623159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001623159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/19/2023 06:06:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,047
            CDSs (total)                      :: 2,001
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 30 of 62
            Pseudo Genes (internal stop)      :: 11 of 62
            Pseudo Genes (multiple problems)  :: 15 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27862179-rid17134413.guided.181"
                     /strain="116"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA"
                     /collection_date="2019"
     gene            <1..>1009
                     /locus_tag="VOX58_002047"
     CDS             <1..>1009
                     /locus_tag="VOX58_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HES5879221.1"
                     /translation="QAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLS
                     AEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIR
                     FQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRL
                     AYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKL
                     NSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDS
                     TTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPN
                     PF"
BASE COUNT          373 a          154 c          189 g          293 t
ORIGIN      
        1 acaagcacaa actcgtatat tatttcatgc acttaggaaa atggggattc ccacaatctt
       61 ttttatcaat aagattgacc aaaatggaat tgatttatca acggtttatc aggatattaa
      121 agagaaactt tctgccgaaa ttgtaatcaa acagaaggta gaactgtatc ctaatatgtg
      181 tgtgacgaac tttaccgaat ctgaacaatg ggatacggta atagagggaa acgatgacct
      241 tttagagaaa tatatgtccg gtaaatcatt agaagcattg gaactcgaac aagaggaaag
      301 cataagattt cagaattgtt ctctgttccc tctttatcat ggaagtgcaa aaagtaatat
      361 agggattgat aaccttatag aagttattac taataaattt tattcatcaa cacatcgagg
      421 tccgtctgaa ctttgcggaa atgttttcaa aattgaatat acaaaaaaaa gacaacgtct
      481 tgcatatata cgcctttata gtggagtact acatttacga gattcggtta gagtatcaga
      541 aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat tatgtaagat
      601 tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga agttaaatag
      661 tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct
      721 actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct
      781 tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga
      841 aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga
      901 aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg agagaccgtt
      961 aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttc
//
